Nell1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nell1
Entrez Gene ID	81733
Full Name	neural EGFL like 1
General protein information	Preferred Names neural EGFL like 1 Names protein kinase C-binding protein NELL1 NEL-like 1 NEL-like protein 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q22
Summary	interacts with and phosphorylated by protein kinase C betaI (PKCbetaI) and is involved in modulation of osteoblast differentiation; overexpression of human homolog is associated with craniosynostosis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017589771.1	XP_017445260.1	protein kinase C-binding protein NELL1 isoform X1
NM_031069.1	NP_112331.1	protein kinase C-binding protein NELL1 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NELL1	XP_534090.3
Mus musculus (house mouse)	Nell1	NP_001032995.1
Xenopus tropicalis (tropical clawed frog)	nell1	XP_004914252.1
Rattus norvegicus (Norway rat)	Nell1	NP_112331.1
Gallus gallus (chicken)	NELL1	XP_001234167.2
Homo sapiens (human)	NELL1	NP_001275642.1
Pan troglodytes (chimpanzee)	NELL1	XP_001173867.3
	NELL1	XP_001092428.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010335	XP_311609.4

Gene Ontology
Bibliography

Related articles in PubMed

Novel Wnt Regulator NEL-Like Molecule-1 Antagonizes Adipogenesis and Augments Osteogenesis Induced by Bone Morphogenetic Protein 2.
Shen J, James AW, Zhang X, Pang S, Zara JN, Asatrian G, Chiang M, Lee M, Khadarian K, Nguyen A, Lee KS, Siu RK, Tetradis S, Ting K, Soo C
The American journal of pathology186(2)419-34(2016 Feb)

Proliferation and osteogenic differentiation of mesenchymal stem cells induced by a short isoform of NELL-1.
Pang S, Shen J, Liu Y, Chen F, Zheng Z, James AW, Hsu CY, Zhang H, Lee KS, Wang C, Li C, Chen X, Jia H, Zhang X, Soo C, Ting K
Stem cells (Dayton, Ohio)33(3)904-15(2015 Mar)

NELL-1 injection maintains long-bone quantity and quality in an ovariectomy-induced osteoporotic senile rat model.
Kwak J, Zara JN, Chiang M, Ngo R, Shen J, James AW, Le KM, Moon C, Zhang X, Gou Z, Ting K, Soo C
Tissue engineering. Part A19(3-4)426-36(2013 Feb)

NELL-1 binds to APR3 affecting human osteoblast proliferation and differentiation.
Zou X, Shen J, Chen F, Ting K, Zheng Z, Pang S, Zara JN, Adams JS, Soo C, Zhang X
FEBS letters585(15)2410-8(2011 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

establish the feasibility of combining NELL-1 with BMP2 to improve clinical bone regeneration and provide mechanistic insight into canonical Wnt pathway activity during NELL-1 and BMP2 osteogenesis
Title: Novel Wnt Regulator NEL-Like Molecule-1 Antagonizes Adipogenesis and Augments Osteogenesis Induced by Bone Morphogenetic Protein 2.

NELL-1570 has the potential to be used for cell-based or hormone-based therapy of bone regeneration.
Title: Proliferation and osteogenic differentiation of mesenchymal stem cells induced by a short isoform of NELL-1.

Thus, NELL-1 effectively enhances in situ osteogenesis in the bone marrow, making it potentially useful in the prevention and treatment of osteoporotic fractures.
Title: NELL-1 injection maintains long-bone quantity and quality in an ovariectomy-induced osteoporotic senile rat model.

Nell-1 can significantly improve bone regeneration in a femoral segmental defect in a dose-dependent manner and is a potent osteospecific growth factor.
Title: Nell-1 enhances bone regeneration in a rat critical-sized femoral segmental defect model.

Nell1 accelerates chondrocyte hypertrophy and endochondral bone formation.
Title: Nell-1 induced bone formation within the distracted intermaxillary suture.

The following Nell1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nell1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa07513	NM_031069.1 Latest version!	Rattus norvegicus neural EGFL like 1 (Nell1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa56866	XM_017589771.1 Latest version!	Rattus norvegicus neural EGFL like 1 (Nell1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nell1 ( NM_031069.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_112331.1 Rattus norvegicus neural EGFL like 1 (Nell1), mRNA.

Price & Availability↑

CloneID	ORa07513
Clone ID Related Accession (Same CDS sequence)	NM_031069.1
Accession Version	NM_031069.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	protein kinase C-binding protein NELL1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U48246.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U48246.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCCGATGG ATGTGATTTT AGTTTTGTGG TTCTGTGTAT GCACCGCCAG GACAGTGTTG 
GGCTTTGGGA TGGACCCTGA CCTTCAGCTG GACATCATCT CAGAGCTCGA CCTGGTGAAC 
ACCACCCTGG GAGTCACGCA GGTGGCTGGA CTGCACAACG CCAGTAAAGC ATTTCTATTT 
CAAGATGTAC AGAGAGAGAT CCATTCGGCC CCTCACGTGA GTGAGAAGCT GATCCAGCTA 
TTCCGGAATA AGAGCGAGTT CACCTTTTTG GCTACAGTGC AGCAGAAACC ATCCACCTCA 
GGGGTGATAC TGTCCATCCG GGAGCTGGAG CACAGCTATT TTGAACTGGA GAGCAGTGGC 
CCAAGAGAAG AGATACGCTA CCATTACATA CATGGTGGAA AGCCCAGGAC TGAGGCCCTT 
CCCTACCGCA TGGCAGACGG ACAATGGCAC AAGGTCGCGC TGTCAGTGAG CGCCTCTCAC 
CTCCTGCTCC ACATCGACTG CAATAGGATT TACGAGCGTG TGATAGACCC TCCGGAGACC 
AACCTTCCTC CAGGAAGCAA TCTGTGGCTT GGGCAACGTA ACCAAAAGCA TGGCTTTTTC 
AAAGGAATCA TCCAAGATGG TAAGATCATC TTCATGCCGA ATGGTTTCAT CACACAGTGT 
CCCAACCTCA ATCGCACTTG CCCAACATGC AGTGACTTCC TGAGCCTGGT TCAAGGAATA 
ATGGATTTGC AAGAGCTTTT GGCCAAGATG ACTGCAAAAC TGAATTATGC AGAGACGAGA 
CTTGGTCAAC TGGAAAATTG CCACTGTGAG AAGACCTGCC AAGTGAGTGG GCTGCTCTAC 
AGGGACCAAG ACTCCTGGGT GGATGGTGAC AACTGTGGGA ACTGCACGTG CAAAAGTGGT 
GCCGTGGAGT GCCGCAGGAT GTCCTGTCCC CCGCTCAACT GTTCCCCGGA CTCACTTCCT 
GTGCACATTT CCGGCCAGTG TTGTAAAGTT TGCAGACCAA AATGTATCTA TGGAGGAAAA 
GTTCTTGCTG AGGGCCAGCG GATTTTAACC AAGACCTGCC GGGAATGTCG AGGTGGAGTC 
TTGGTAAAAA TCACAGAAGC TTGCCCTCCT TTGAACTGCT CAGCAAAGGA TCATATTCTT 
CCAGAGAATC AGTGCTGCAG GGTCTGCCCA GGTCATAACT TCTGTGCAGA AGCACCTAAG 
TGCGGAGAAA ACTCGGAATG CAAAAATTGG AATACAAAAG CAACCTGTGA GTGCAAGAAT 
GGATACATCT CTGTCCAGGG CAACTCTGCA TACTGTGAAG ATATTGATGA GTGTGCAGCT 
AAAATGCACT ATTGTCATGC CAACACCGTG TGTGTCAACT TGCCGGGGTT GTATCGCTGT 
GACTGCGTCC CAGGGTACAT CCGTGTGGAT GACTTCTCTT GTACGGAGCA TGATGATTGT 
GGCAGCGGAC AACACAACTG CGACAAAAAT GCCATCTGTA CCAACACAGT CCAGGGACAC 
AGCTGCACCT GCCAGCCGGG TTACGTGGGA AATGGCACCA TCTGCAAAGC ATTCTGTGAA 
GAGGGTTGCA GATACGGAGG TACCTGTGTG GCTCCTAACA AGTGTGTCTG TCCTTCTGGA 
TTCACGGGAA GCCACTGTGA GAAAGATATT GATGAATGCG CAGAGGGATT CGTTGAATGC 
CACAACTACT CCCGCTGTGT TAACCTGCCA GGGTGGTACC ACTGTGAGTG CAGAAGCGGT 
TTCCATGACG ATGGGACCTA CTCACTGTCC GGGGAGTCCT GCATTGATAT CGATGAATGT 
GCCTTAAGAA CTCACACTTG TTGGAATGAC TCTGCCTGCA TCAACTTAGC AGGAGGATTT 
GACTGCCTGT GTCCCTCTGG GCCCTCCTGC TCTGGTGACT GTCCCCACGA AGGAGGGCTG 
AAGCATAATG GGCAGGTGTG GATTCTGAGA GAAGACAGGT GTTCAGTCTG TTCCTGCAAG 
GATGGGAAGA TATTCTGCCG GCGGACAGCT TGTGATTGCC AGAATCCAAA TGTTGACCTT 
TTTTGCTGCC CAGAGTGCGA TACCAGGGTC ACCAGCCAAT GTTTAGATCA AAGTGGACAG 
AAGCTCTATC GAAGTGGAGA CAACTGGACC CACAGCTGCC AGCAGTGCCG ATGTCTGGAA 
GGAGAGGCAG ACTGCTGGCC TCTGGCTTGC CCTAGTTTGG GCTGTGAATA CACAGCCATG 
TTTGAAGGGG AGTGTTGTCC CCGATGTGTC AGTGACCCCT GCCTGGCTGG TAATATTGCC 
TATGACATCA GAAAAACTTG CCTGGACAGC TTTGGTGTTT CGAGGCTGAG CGGAGCCGTG 
TGGACAATGG CTGGATCTCC TTGTACAACC TGCAAATGCA AGAATGGGAG AGTCTGCTGC 
TCTGTGGATC TGGAGTGTAT TGAGAATAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112331.1
CDS	59..2491
Translation	MPMDVILVLWFCVCTARTVLGFGMDPDLQLDIISELDLVNTTLGVTQVAGLHNASKAFLFQDVQREIHSAPHVSEKLIQLFRNKSEFTFLATVQQKPSTSGVILSIRELEHSYFELESSGPREEIRYHYIHGGKPRTEALPYRMADGQWHKVALSVSASHLLLHIDCNRIYERVIDPPETNLPPGSNLWLGQRNQKHGFFKGIIQDGKIIFMPNGFITQCPNLNRTCPTCSDFLSLVQGIMDLQELLAKMTAKLNYAETRLGQLENCHCEKTCQVSGLLYRDQDSWVDGDNCGNCTCKSGAVECRRMSCPPLNCSPDSLPVHISGQCCKVCRPKCIYGGKVLAEGQRILTKTCRECRGGVLVKITEACPPLNCSAKDHILPENQCCRVCPGHNFCAEAPKCGENSECKNWNTKATCECKNGYISVQGNSAYCEDIDECAAKMHYCHANTVCVNLPGLYRCDCVPGYIRVDDFSCTEHDDCGSGQHNCDKNAICTNTVQGHSCTCQPGYVGNGTICKAFCEEGCRYGGTCVAPNKCVCPSGFTGSHCEKDIDECAEGFVECHNYSRCVNLPGWYHCECRSGFHDDGTYSLSGESCIDIDECALRTHTCWNDSACINLAGGFDCLCPSGPSCSGDCPHEGGLKHNGQVWILREDRCSVCSCKDGKIFCRRTACDCQNPNVDLFCCPECDTRVTSQCLDQSGQKLYRSGDNWTHSCQQCRCLEGEADCWPLACPSLGCEYTAMFEGECCPRCVSDPCLAGNIAYDIRKTCLDSFGVSRLSGAVWTMAGSPCTTCKCKNGRVCCSVDLECIENN

Target ORF information:

RefSeq Version	NM_031069.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neural EGFL like 1 (Nell1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031069.1

ORF Insert Sequence:

ATGCCGATGG ATGTGATTTT AGTTTTGTGG TTCTGTGTAT GCACCGCCAG GACAGTGTTG 
GGCTTTGGGA TGGACCCTGA CCTTCAGCTG GACATCATCT CAGAGCTCGA CCTGGTGAAC 
ACCACCCTGG GAGTCACGCA GGTGGCTGGA CTGCACAACG CCAGTAAAGC ATTTCTATTT 
CAAGATGTAC AGAGAGAGAT CCATTCGGCC CCTCACGTGA GTGAGAAGCT GATCCAGCTA 
TTCCGGAATA AGAGCGAGTT CACCTTTTTG GCTACAGTGC AGCAGAAACC ATCCACCTCA 
GGGGTGATAC TGTCCATCCG GGAGCTGGAG CACAGCTATT TTGAACTGGA GAGCAGTGGC 
CCAAGAGAAG AGATACGCTA CCATTACATA CATGGTGGAA AGCCCAGGAC TGAGGCCCTT 
CCCTACCGCA TGGCAGACGG ACAATGGCAC AAGGTCGCGC TGTCAGTGAG CGCCTCTCAC 
CTCCTGCTCC ACATCGACTG CAATAGGATT TACGAGCGTG TGATAGACCC TCCGGAGACC 
AACCTTCCTC CAGGAAGCAA TCTGTGGCTT GGGCAACGTA ACCAAAAGCA TGGCTTTTTC 
AAAGGAATCA TCCAAGATGG TAAGATCATC TTCATGCCGA ATGGTTTCAT CACACAGTGT 
CCCAACCTCA ATCGCACTTG CCCAACATGC AGTGACTTCC TGAGCCTGGT TCAAGGAATA 
ATGGATTTGC AAGAGCTTTT GGCCAAGATG ACTGCAAAAC TGAATTATGC AGAGACGAGA 
CTTGGTCAAC TGGAAAATTG CCACTGTGAG AAGACCTGCC AAGTGAGTGG GCTGCTCTAC 
AGGGACCAAG ACTCCTGGGT GGATGGTGAC AACTGTGGGA ACTGCACGTG CAAAAGTGGT 
GCCGTGGAGT GCCGCAGGAT GTCCTGTCCC CCGCTCAACT GTTCCCCGGA CTCACTTCCT 
GTGCACATTT CCGGCCAGTG TTGTAAAGTT TGCAGACCAA AATGTATCTA TGGAGGAAAA 
GTTCTTGCTG AGGGCCAGCG GATTTTAACC AAGACCTGCC GGGAATGTCG AGGTGGAGTC 
TTGGTAAAAA TCACAGAAGC TTGCCCTCCT TTGAACTGCT CAGCAAAGGA TCATATTCTT 
CCAGAGAATC AGTGCTGCAG GGTCTGCCCA GGTCATAACT TCTGTGCAGA AGCACCTAAG 
TGCGGAGAAA ACTCGGAATG CAAAAATTGG AATACAAAAG CAACCTGTGA GTGCAAGAAT 
GGATACATCT CTGTCCAGGG CAACTCTGCA TACTGTGAAG ATATTGATGA GTGTGCAGCT 
AAAATGCACT ATTGTCATGC CAACACCGTG TGTGTCAACT TGCCGGGGTT GTATCGCTGT 
GACTGCGTCC CAGGGTACAT CCGTGTGGAT GACTTCTCTT GTACGGAGCA TGATGATTGT 
GGCAGCGGAC AACACAACTG CGACAAAAAT GCCATCTGTA CCAACACAGT CCAGGGACAC 
AGCTGCACCT GCCAGCCGGG TTACGTGGGA AATGGCACCA TCTGCAAAGC ATTCTGTGAA 
GAGGGTTGCA GATACGGAGG TACCTGTGTG GCTCCTAACA AGTGTGTCTG TCCTTCTGGA 
TTCACGGGAA GCCACTGTGA GAAAGATATT GATGAATGCG CAGAGGGATT CGTTGAATGC 
CACAACTACT CCCGCTGTGT TAACCTGCCA GGGTGGTACC ACTGTGAGTG CAGAAGCGGT 
TTCCATGACG ATGGGACCTA CTCACTGTCC GGGGAGTCCT GCATTGATAT CGATGAATGT 
GCCTTAAGAA CTCACACTTG TTGGAATGAC TCTGCCTGCA TCAACTTAGC AGGAGGATTT 
GACTGCCTGT GTCCCTCTGG GCCCTCCTGC TCTGGTGACT GTCCCCACGA AGGAGGGCTG 
AAGCATAATG GGCAGGTGTG GATTCTGAGA GAAGACAGGT GTTCAGTCTG TTCCTGCAAG 
GATGGGAAGA TATTCTGCCG GCGGACAGCT TGTGATTGCC AGAATCCAAA TGTTGACCTT 
TTTTGCTGCC CAGAGTGCGA TACCAGGGTC ACCAGCCAAT GTTTAGATCA AAGTGGACAG 
AAGCTCTATC GAAGTGGAGA CAACTGGACC CACAGCTGCC AGCAGTGCCG ATGTCTGGAA 
GGAGAGGCAG ACTGCTGGCC TCTGGCTTGC CCTAGTTTGG GCTGTGAATA CACAGCCATG 
TTTGAAGGGG AGTGTTGTCC CCGATGTGTC AGTGACCCCT GCCTGGCTGG TAATATTGCC 
TATGACATCA GAAAAACTTG CCTGGACAGC TTTGGTGTTT CGAGGCTGAG CGGAGCCGTG 
TGGACAATGG CTGGATCTCC TTGTACAACC TGCAAATGCA AGAATGGGAG AGTCTGCTGC 
TCTGTGGATC TGGAGTGTAT TGAGAATAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nell1 ( XM_017589771.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017445260.1 Rattus norvegicus neural EGFL like 1 (Nell1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa56866
Clone ID Related Accession (Same CDS sequence)	XM_017589771.1
Accession Version	XM_017589771.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein kinase C-binding protein NELL1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGATGG ATGTGATTTT AGTTTTGTGG TTCTGTGTAT GCACCGCCAG GACAGTGTTG 
GGCTTTGGGA TGGACCCTGA CCTTCAGCTG GACATCATCT CAGAGCTCGA CCTGGTGAAC 
ACCACCCTGG GAGTCACGCA GGTGGCTGGA CTGCACAACG CCAGTAAAGC ATTTCTATTT 
CAAGATGTAC AGAGAGAGAT CCATTCGGCC CCTCACGTGA GTGAGAAGCT GATCCAGCTA 
TTCCGAAATA AGAGCGAGTT CACCTTTTTG GCTACAGTGC AGCAGAAACC ATCCACCTCA 
GGGGTGATAC TGTCCATCCG GGAGCTGGAG CACAGCTATT TTGAACTGGA GAGCAGTGGC 
CCAAGAGAAG AGATACGCTA CCATTACATA CATGGTGGAA AGCCCAGGAC TGAGGCCCTT 
CCCTACCGCA TGGCAGACGG ACAATGGCAC AAGGTCGCGC TGTCAGTGAG CGCCTCTCAC 
CTCCTGCTCC ACATCGACTG CAATAGGATT TACGAGCGTG TGATAGACCC TCCGGAGACC 
AACCTTCCTC CAGGAAGCAA TCTGTGGCTT GGGCAACGTA ACCAAAAGCA TGGCTTTTTC 
AAAGGAATCA TCCAAGATGG TAAGATCATC TTCATGCCGA ATGGTTTCAT CACACAGTGT 
CCCAACCTCA ATCGCACTTG CCCAACATGC AGTGACTTCC TGAGCCTGGT TCAAGGAATA 
ATGGATTTGC AAGAGCTTTT GGCCAAGATG ACTGCAAAAC TGAATTATGC AGAGACGAGA 
CTTGGTCAAC TGGAAAATTG CCACTGTGAG AAGACCTGCC AAGTGAGTGG GCTGCTCTAC 
AGGGACCAAG ACTCCTGGGT GGATGGTGAC AACTGTAGGA ACTGCACGTG CAAAAGTGGT 
GCCGTGGAGT GCCGCAGGAT GTCCTGTCCC CCGCTCAACT GTTCCCCAGA CTCACTTCCT 
GTGCACATTT CCGGCCAGTG TTGTAAAGTT TGCAGACCAA AATGTATCTA TGGAGGAAAA 
GTTCTTGCTG AGGGCCAGCG GATTTTAACC AAGACCTGCC GGGAATGTCG AGGTGGAGTC 
TTGGTAAAAA TCACAGAAGC TTGCCCTCCT TTGAACTGCT CAGCAAAGGA TCATATTCTT 
CCAGAGAATC AGTGCTGCAG GGTCTGCCCA GGTCATAACT TCTGTGCAGA AGCACCTAAG 
TGCGGAGAAA ACTCGGAATG CAAAAATTGG AATACAAAAG CAACCTGTGA GTGCAAGAAT 
GGATACATCT CTGTCCAGGG CAACTCTGCA TACTGTGAAG ATATTGATGA GTGTGCAGCT 
AAAATGCACT ATTGTCATGC CAACACCGTG TGTGTCAACT TGCCGGGGTT GTATCGCTGT 
GACTGCGTCC CAGGATACAT CCGTGTGGAT GACTTCTCTT GTACGGAGCA TGATGATTGT 
GGCAGCGGAC AACACAACTG CGACAAAAAT GCCATCTGTA CCAACACAGT CCAGGGACAC 
AGCTGCACCT GCCAGCCGGG TTACGTGGGA AATGGCACCA TCTGCAAAGC ATTCTGTGAA 
GAGGGTTGCA GATACGGAGG TACCTGTGTG GCTCCTAACA AGTGTGTCTG TCCTTCTGGA 
TTCACGGGAA GCCACTGTGA GAAAGATATC GATGAATGTG CCTTAAGAAC TCACACTTGT 
TGGAATGACT CTGCCTGCAT CAACTTAGCA GGAGGATTTG ACTGCCTGTG TCCCTCTGGG 
CCCTCCTGCT CTGGTGACTG TCCCCACGAA GGAGGGCTGA AGCATAATGG GCAGGTGTGG 
ATTCTGAGAG AAGACAGGTG TTCAGTCTGT TCCTGCAAGG ATGGGAAGAT ATTCTGCCGG 
CGGACAGCTT GTGATTGCCA GAATCCAAAT GTTGACCTTT TTTGCTGCCC AGAGTGCGAT 
ACCAGGGTCA CCAGCCAATG TTTAGATCAA AGTGGACAGA AGCTCTATCG AAGTGGAGAC 
AACTGGACCC ACAGCTGCCA GCAGTGCCGA TGTCTGGAAG GAGAGGCAGA CTGCTGGCCT 
CTGGCTTGCC CTAGTTTGGG CTGTGAATAC ACAGCCATGT TTGAAGGGGA GTGTTGTCCC 
CGATGTGTCA GTGACCCCTG CCTGGCTGAT AATATTGCCT ATGACATCAG AAAAACTTGC 
CTGGACAGCT TTGGTGTTTC GAGGCTGAGC GGAGCCGTGT GGACAATGGC TGGATCTCCT 
TGTACAACCT GCAAATGCAA GAATGGGAGA GTCTGCTGCT CTGTGGATCT GGAGTGTATT 
GAGAATAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017445260.1
CDS	551..2842
Translation	MPMDVILVLWFCVCTARTVLGFGMDPDLQLDIISELDLVNTTLGVTQVAGLHNASKAFLFQDVQREIHSAPHVSEKLIQLFRNKSEFTFLATVQQKPSTSGVILSIRELEHSYFELESSGPREEIRYHYIHGGKPRTEALPYRMADGQWHKVALSVSASHLLLHIDCNRIYERVIDPPETNLPPGSNLWLGQRNQKHGFFKGIIQDGKIIFMPNGFITQCPNLNRTCPTCSDFLSLVQGIMDLQELLAKMTAKLNYAETRLGQLENCHCEKTCQVSGLLYRDQDSWVDGDNCRNCTCKSGAVECRRMSCPPLNCSPDSLPVHISGQCCKVCRPKCIYGGKVLAEGQRILTKTCRECRGGVLVKITEACPPLNCSAKDHILPENQCCRVCPGHNFCAEAPKCGENSECKNWNTKATCECKNGYISVQGNSAYCEDIDECAAKMHYCHANTVCVNLPGLYRCDCVPGYIRVDDFSCTEHDDCGSGQHNCDKNAICTNTVQGHSCTCQPGYVGNGTICKAFCEEGCRYGGTCVAPNKCVCPSGFTGSHCEKDIDECALRTHTCWNDSACINLAGGFDCLCPSGPSCSGDCPHEGGLKHNGQVWILREDRCSVCSCKDGKIFCRRTACDCQNPNVDLFCCPECDTRVTSQCLDQSGQKLYRSGDNWTHSCQQCRCLEGEADCWPLACPSLGCEYTAMFEGECCPRCVSDPCLADNIAYDIRKTCLDSFGVSRLSGAVWTMAGSPCTTCKCKNGRVCCSVDLECIENN

Target ORF information:

RefSeq Version	XM_017589771.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neural EGFL like 1 (Nell1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589771.1

ORF Insert Sequence:

ATGCCGATGG ATGTGATTTT AGTTTTGTGG TTCTGTGTAT GCACCGCCAG GACAGTGTTG 
GGCTTTGGGA TGGACCCTGA CCTTCAGCTG GACATCATCT CAGAGCTCGA CCTGGTGAAC 
ACCACCCTGG GAGTCACGCA GGTGGCTGGA CTGCACAACG CCAGTAAAGC ATTTCTATTT 
CAAGATGTAC AGAGAGAGAT CCATTCGGCC CCTCACGTGA GTGAGAAGCT GATCCAGCTA 
TTCCGAAATA AGAGCGAGTT CACCTTTTTG GCTACAGTGC AGCAGAAACC ATCCACCTCA 
GGGGTGATAC TGTCCATCCG GGAGCTGGAG CACAGCTATT TTGAACTGGA GAGCAGTGGC 
CCAAGAGAAG AGATACGCTA CCATTACATA CATGGTGGAA AGCCCAGGAC TGAGGCCCTT 
CCCTACCGCA TGGCAGACGG ACAATGGCAC AAGGTCGCGC TGTCAGTGAG CGCCTCTCAC 
CTCCTGCTCC ACATCGACTG CAATAGGATT TACGAGCGTG TGATAGACCC TCCGGAGACC 
AACCTTCCTC CAGGAAGCAA TCTGTGGCTT GGGCAACGTA ACCAAAAGCA TGGCTTTTTC 
AAAGGAATCA TCCAAGATGG TAAGATCATC TTCATGCCGA ATGGTTTCAT CACACAGTGT 
CCCAACCTCA ATCGCACTTG CCCAACATGC AGTGACTTCC TGAGCCTGGT TCAAGGAATA 
ATGGATTTGC AAGAGCTTTT GGCCAAGATG ACTGCAAAAC TGAATTATGC AGAGACGAGA 
CTTGGTCAAC TGGAAAATTG CCACTGTGAG AAGACCTGCC AAGTGAGTGG GCTGCTCTAC 
AGGGACCAAG ACTCCTGGGT GGATGGTGAC AACTGTAGGA ACTGCACGTG CAAAAGTGGT 
GCCGTGGAGT GCCGCAGGAT GTCCTGTCCC CCGCTCAACT GTTCCCCAGA CTCACTTCCT 
GTGCACATTT CCGGCCAGTG TTGTAAAGTT TGCAGACCAA AATGTATCTA TGGAGGAAAA 
GTTCTTGCTG AGGGCCAGCG GATTTTAACC AAGACCTGCC GGGAATGTCG AGGTGGAGTC 
TTGGTAAAAA TCACAGAAGC TTGCCCTCCT TTGAACTGCT CAGCAAAGGA TCATATTCTT 
CCAGAGAATC AGTGCTGCAG GGTCTGCCCA GGTCATAACT TCTGTGCAGA AGCACCTAAG 
TGCGGAGAAA ACTCGGAATG CAAAAATTGG AATACAAAAG CAACCTGTGA GTGCAAGAAT 
GGATACATCT CTGTCCAGGG CAACTCTGCA TACTGTGAAG ATATTGATGA GTGTGCAGCT 
AAAATGCACT ATTGTCATGC CAACACCGTG TGTGTCAACT TGCCGGGGTT GTATCGCTGT 
GACTGCGTCC CAGGATACAT CCGTGTGGAT GACTTCTCTT GTACGGAGCA TGATGATTGT 
GGCAGCGGAC AACACAACTG CGACAAAAAT GCCATCTGTA CCAACACAGT CCAGGGACAC 
AGCTGCACCT GCCAGCCGGG TTACGTGGGA AATGGCACCA TCTGCAAAGC ATTCTGTGAA 
GAGGGTTGCA GATACGGAGG TACCTGTGTG GCTCCTAACA AGTGTGTCTG TCCTTCTGGA 
TTCACGGGAA GCCACTGTGA GAAAGATATC GATGAATGTG CCTTAAGAAC TCACACTTGT 
TGGAATGACT CTGCCTGCAT CAACTTAGCA GGAGGATTTG ACTGCCTGTG TCCCTCTGGG 
CCCTCCTGCT CTGGTGACTG TCCCCACGAA GGAGGGCTGA AGCATAATGG GCAGGTGTGG 
ATTCTGAGAG AAGACAGGTG TTCAGTCTGT TCCTGCAAGG ATGGGAAGAT ATTCTGCCGG 
CGGACAGCTT GTGATTGCCA GAATCCAAAT GTTGACCTTT TTTGCTGCCC AGAGTGCGAT 
ACCAGGGTCA CCAGCCAATG TTTAGATCAA AGTGGACAGA AGCTCTATCG AAGTGGAGAC 
AACTGGACCC ACAGCTGCCA GCAGTGCCGA TGTCTGGAAG GAGAGGCAGA CTGCTGGCCT 
CTGGCTTGCC CTAGTTTGGG CTGTGAATAC ACAGCCATGT TTGAAGGGGA GTGTTGTCCC 
CGATGTGTCA GTGACCCCTG CCTGGCTGAT AATATTGCCT ATGACATCAG AAAAACTTGC 
CTGGACAGCT TTGGTGTTTC GAGGCTGAGC GGAGCCGTGT GGACAATGGC TGGATCTCCT 
TGTACAACCT GCAAATGCAA GAATGGGAGA GTCTGCTGCT CTGTGGATCT GGAGTGTATT 
GAGAATAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Novel Wnt Regulator NEL-Like Molecule-1 Antagonizes Adipogenesis and Augments Osteogenesis Induced by Bone Morphogenetic Protein 2.
The American journal of pathology186(2)419-34(2016 Feb)
Shen J,James AW,Zhang X,Pang S,Zara JN,Asatrian G,Chiang M,Lee M,Khadarian K,Nguyen A,Lee KS,Siu RK,Tetradis S,Ting K,Soo C

Proliferation and osteogenic differentiation of mesenchymal stem cells induced by a short isoform of NELL-1.
Stem cells (Dayton, Ohio)33(3)904-15(2015 Mar)
Pang S,Shen J,Liu Y,Chen F,Zheng Z,James AW,Hsu CY,Zhang H,Lee KS,Wang C,Li C,Chen X,Jia H,Zhang X,Soo C,Ting K

NELL-1 injection maintains long-bone quantity and quality in an ovariectomy-induced osteoporotic senile rat model.
Tissue engineering. Part A19(3-4)426-36(2013 Feb)
Kwak J,Zara JN,Chiang M,Ngo R,Shen J,James AW,Le KM,Moon C,Zhang X,Gou Z,Ting K,Soo C

NELL-1 binds to APR3 affecting human osteoblast proliferation and differentiation.
FEBS letters585(15)2410-8(2011 Aug)
Zou X,Shen J,Chen F,Ting K,Zheng Z,Pang S,Zara JN,Adams JS,Soo C,Zhang X

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Nell1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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