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Spag4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Summary

Gene Symbol Spag4
Entrez Gene ID 83623
Full Name sperm associated antigen 4
General protein information
Preferred Names

sperm associated antigen 4

Names

sperm-associated antigen 4 protein
SUN domain-containing protein 4
outer dense fiber-associated protein SPAG4
sperm antigen 4
Gene Type protein-coding
Organism Rattus norvegicus(Norway rat)
Genome

3

3q42
Summary spermatid-specific structural protein; interacts with an outer dense fiber (Odf1) localized to the sperm tail via a leucine zipper motif [RGD, Feb 2006].

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_008762386.2 XP_008760608.1 sperm-associated antigen 4 protein isoform X1
NM_031792.1 NP_113980.1 sperm-associated antigen 4 protein

Pathways from BioSystems


Homologies


Rattus norvegicus (Norway rat) LOC100911109 XP_003749659.1
Mus musculus (house mouse) Spag4 NP_631890.3
Homo sapiens (human) SPAG4 NP_003107.1
Pan troglodytes (chimpanzee) SPAG4 XP_514609.2
SPAG4 XP_001098382.1
Canis lupus familiaris (dog) SPAG4 XP_542978.2
Bos taurus (cattle) SPAG4 NP_001069975.1
Rattus norvegicus (Norway rat) Spag4 NP_113980.1

Gene Ontology


Bibliography

Related articles in PubMed

Spag4, a novel sperm protein, binds outer dense-fiber protein Odf1 and localizes to microtubules of manchette and axoneme.
Shao X, Tarnasky HA, Lee JP, Oko R, van der Hoorn FA
Developmental biology211(1)109-23(1999 Jul)

A novel testis-specific gene, SPAG4, whose product interacts specifically with outer dense fiber protein ODF27, maps to human chromosome 20q11.2.
Tarnasky H, Gill D, Murthy S, Shao X, Demetrick DJ, van der Hoorn FA
Cytogenetics and cell genetics81(1)65-7(1998)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Spag4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spag4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa08748 NM_031792.1
Latest version!		 Rattus norvegicus sperm associated antigen 4 (Spag4), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
ORa43770 XM_008762386.2
Latest version!		 Rattus norvegicus sperm associated antigen 4 (Spag4), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID ORa08748
Clone ID Related Accession (Same CDS sequence) NM_031792.1
Accession Version NM_031792.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1335bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-11-10
Organism Rattus norvegicus(Norway rat)
Product sperm-associated antigen 4 protein
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF043345.2. ##Evidence-Data-START## Transcript exon combination :: AF043345.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689604, SAMN00632250 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGCGGCGGA ACCCCCGCCC AGGCTCGGCC GCATCCTCCC ACAATCACAC ACCCAACTTC 
TACAGCGAGA ACAGCAATAG TTCCCACAGC GCGACTTCGG GGGACAGCAA TGGGCGCCGG 
TCCGCTGGGC CGGAGCTCGG GGAGCCCGAT GGCAGAATGG CCCGGGGCTC CAGCTGTGGT 
GAGCCCGCCT TAAGCTCAGG AGTGCCCGGA GGAGACACAT GGGCAGGAAG CTCTCGGCCG 
AAGCTGGCGC CTCGGAGCCA CAATGGGCAG ACCGCCTGTG GCGCGGCAAC CGTGAGGGGC 
GGGGCCTCGG AACCGTCTGG GTCTCCTGCA GTCTTGGAGG AACAACTCAA CCTTCTCCCG 
ATACTGGATC TGAGGCAGGA GATGCCTCCC CCGCCGGTGT CCAAGAGCTT CCTGAGCCTC 
TTCTTTCAGG TGCTCAGCGT GTTTTTATCC TTGGTAGCCG ACGGGCTGGT CTGTGTGTAC 
AGGGAAATCT GCTCCATCCG CTTCCTGTTC ACCGCTGTGT CGCTCCTGAG CATTTTTCTG 
GCAGCACTCT GGTGGGGGCT CCTGTACCTG ATCCCTCCTT TGGAGAATGA ACCTAAGGAG 
ATGCTGACTC TAAGCCAATA CCACCACCGC GTGCACTCCC AGGGCCAGCA ACTGCAGCAG 
CTCCAGGCAG AACTGAGTAA ACTCCACAAG GAGGTGACCA GTGTTCGTGC AGCCCACAGT 
GAGAGAGTGG CCAAGCTCGT GTTCCAGAGG CTGAATGAGG ACTTCGTGCG CAAGCCTGAC 
TATGCATTGA GCTCTGTGGG AGCCTCCATC GACCTGGAGA AGACATCCAG TGACTACGAG 
GACCGGAATA CTGCCTACTT CTGGAACCGA TTAAGCTTCT GGAACTACGC CCGCCCACCC 
TCTGTCATAT TGGAGCCAGA TGTGTTCCCT GGAAATTGCT GGGCTTTTGA AGGTGAGCAA 
GGCCAGGTGG TGATCCGACT GCCAGGTCAT GTGCAGTTAA GCGACATCAC CCTGCAGCAC 
CCTCCACCCA CTGTGGCACA CACCGGAGGA GCCAGCAGTG CGCCCCGGGA CTTCGCAGTC 
TTTGGGCTCC AAGCTGATGA TGACGAGACT GAAGTGTTCT TGGGAAAATT CATCTTTGAA 
GTGCAGAAAT CTGAAATTCA GACTTTCCAC CTCCAGAATG ACCCTCCATC TGCCTTCCCC 
AAGGTGAAGA TTCAGATTTT AAGCAACTGG GGCCACCCAC GCTTCACATG CTTATATCGA 
GTCCGTGCCC ATGGTGTGCG GATTTCAGAG TCGGCAGAGG ATAATGCCAT GGGGGTCACA 
GGGGGACCCC ATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_113980.1
CDS105..1439
Translation

Target ORF information:

RefSeq Version NM_031792.1
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus sperm associated antigen 4 (Spag4), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_031792.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGCGGCGGA ACCCCCGCCC AGGCTCGGCC GCATCCTCCC ACAATCACAC ACCCAACTTC 
TACAGCGAGA ACAGCAATAG TTCCCACAGC GCGACTTCGG GGGACAGCAA TGGGCGCCGG 
TCCGCTGGGC CGGAGCTCGG GGAGCCCGAT GGCAGAATGG CCCGGGGCTC CAGCTGTGGT 
GAGCCCGCCT TAAGCTCAGG AGTGCCCGGA GGAGACACAT GGGCAGGAAG CTCTCGGCCG 
AAGCTGGCGC CTCGGAGCCA CAATGGGCAG ACCGCCTGTG GCGCGGCAAC CGTGAGGGGC 
GGGGCCTCGG AACCGTCTGG GTCTCCTGCA GTCTTGGAGG AACAACTCAA CCTTCTCCCG 
ATACTGGATC TGAGGCAGGA GATGCCTCCC CCGCCGGTGT CCAAGAGCTT CCTGAGCCTC 
TTCTTTCAGG TGCTCAGCGT GTTTTTATCC TTGGTAGCCG ACGGGCTGGT CTGTGTGTAC 
AGGGAAATCT GCTCCATCCG CTTCCTGTTC ACCGCTGTGT CGCTCCTGAG CATTTTTCTG 
GCAGCACTCT GGTGGGGGCT CCTGTACCTG ATCCCTCCTT TGGAGAATGA ACCTAAGGAG 
ATGCTGACTC TAAGCCAATA CCACCACCGC GTGCACTCCC AGGGCCAGCA ACTGCAGCAG 
CTCCAGGCAG AACTGAGTAA ACTCCACAAG GAGGTGACCA GTGTTCGTGC AGCCCACAGT 
GAGAGAGTGG CCAAGCTCGT GTTCCAGAGG CTGAATGAGG ACTTCGTGCG CAAGCCTGAC 
TATGCATTGA GCTCTGTGGG AGCCTCCATC GACCTGGAGA AGACATCCAG TGACTACGAG 
GACCGGAATA CTGCCTACTT CTGGAACCGA TTAAGCTTCT GGAACTACGC CCGCCCACCC 
TCTGTCATAT TGGAGCCAGA TGTGTTCCCT GGAAATTGCT GGGCTTTTGA AGGTGAGCAA 
GGCCAGGTGG TGATCCGACT GCCAGGTCAT GTGCAGTTAA GCGACATCAC CCTGCAGCAC 
CCTCCACCCA CTGTGGCACA CACCGGAGGA GCCAGCAGTG CGCCCCGGGA CTTCGCAGTC 
TTTGGGCTCC AAGCTGATGA TGACGAGACT GAAGTGTTCT TGGGAAAATT CATCTTTGAA 
GTGCAGAAAT CTGAAATTCA GACTTTCCAC CTCCAGAATG ACCCTCCATC TGCCTTCCCC 
AAGGTGAAGA TTCAGATTTT AAGCAACTGG GGCCACCCAC GCTTCACATG CTTATATCGA 
GTCCGTGCCC ATGGTGTGCG GATTTCAGAG TCGGCAGAGG ATAATGCCAT GGGGGTCACA 
GGGGGACCCC ATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa43770
Clone ID Related Accession (Same CDS sequence) XM_008762386.2
Accession Version XM_008762386.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1098bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product sperm-associated antigen 4 protein isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008762386.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGGCAGGA AGCTCTCGGC CGAAGCTGGC GCCTCGGAGC CACAATGGGC AGACCGCCTG 
TGGCGCGGCA ACCAACCGTC TGGGTCTCCT GCAGTCTTGG AGGAACAACT CAACCTTCTC 
CCGATACTGG ATCTGAGGCA GGAGATGCCT CCCCCGCCGG TGTCCAAGAG CTTCCTGAGC 
CTCTTCTTTC AGGTGCTCAG CGTGTTTTTA TCCTTGGTAG CCGACGGGCT GGTCTGTGTG 
TACAGGGAAA TCTGCTCCAT CCGCTTCCTG TTCACCGCTG TGTCGCTCCT GAGCATTTTT 
CTGGCAGCAC TCTGGTGGGG GCTCCTGTAC CTGATCCCTC CTTTGGAGAA TGAACCTAAG 
GAGATGCTGA CTCTAAGCCA ATACCACCAC CGCGTGCACT CCCAGGGCCA GCAACTGCAG 
CAGCTCCAGG CAGAACTGAG TAAACTCCAC AAGGAGGTGA CCAGTGTTCG TGCAGCCCAC 
AGTGAGAGAG TGGCCAAGCT CGTGTTCCAG AGGCTGAATG AGGACTTCGT GCGCAAGCCT 
GACTATGCAT TGAGCTCTGT GGGAGCCTCC ATCGACCTGG AGAAGACATC CAGTGACTAC 
GAGGACCGGA ATACTGCCTA CTTCTGGAAC CGATTAAGCT TCTGGAACTA CGCCCGCCCA 
CCCTCTGTCA TATTGGAGCC AGATGTGTTC CCTGGAAATT GCTGGGCTTT TGAAGGTGAG 
CAAGGCCAGG TGGTGATCCG ACTGCCAGGT CATGTGCAGT TAAGCGACAT CACCCTGCAG 
CACCCTCCAC CCACTGTGGC ACACACCGGA GGAGCCAGCA GTGCGCCCCG GGACTTCGCA 
GTCTTTGGGC TCCAAGCTGA TGATGACGAG ACTGAAGTGT TCTTGGGAAA ATTCATCTTT 
GAAGTGCAGA AATCTGAAAT TCAGACTTTC CACCTCCAGA ATGACCCTCC ATCTGCCTTC 
CCCAAGGTGA AGATTCAGAT TCTAAGCAAC TGGGGCCACC CACGCTTCAC ATGCTTATAT 
CGAGTCCGTG CCCATGGTGT GCGGATCTCA GAGTCGGCAG AGGATAATGC CATGGGGGTC 
ACAGGGGGAC CCCATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_008760608.1
CDS104..1201
Translation

Target ORF information:

RefSeq Version XM_008762386.2
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus sperm associated antigen 4 (Spag4), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_008762386.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGGCAGGA AGCTCTCGGC CGAAGCTGGC GCCTCGGAGC CACAATGGGC AGACCGCCTG 
TGGCGCGGCA ACCAACCGTC TGGGTCTCCT GCAGTCTTGG AGGAACAACT CAACCTTCTC 
CCGATACTGG ATCTGAGGCA GGAGATGCCT CCCCCGCCGG TGTCCAAGAG CTTCCTGAGC 
CTCTTCTTTC AGGTGCTCAG CGTGTTTTTA TCCTTGGTAG CCGACGGGCT GGTCTGTGTG 
TACAGGGAAA TCTGCTCCAT CCGCTTCCTG TTCACCGCTG TGTCGCTCCT GAGCATTTTT 
CTGGCAGCAC TCTGGTGGGG GCTCCTGTAC CTGATCCCTC CTTTGGAGAA TGAACCTAAG 
GAGATGCTGA CTCTAAGCCA ATACCACCAC CGCGTGCACT CCCAGGGCCA GCAACTGCAG 
CAGCTCCAGG CAGAACTGAG TAAACTCCAC AAGGAGGTGA CCAGTGTTCG TGCAGCCCAC 
AGTGAGAGAG TGGCCAAGCT CGTGTTCCAG AGGCTGAATG AGGACTTCGT GCGCAAGCCT 
GACTATGCAT TGAGCTCTGT GGGAGCCTCC ATCGACCTGG AGAAGACATC CAGTGACTAC 
GAGGACCGGA ATACTGCCTA CTTCTGGAAC CGATTAAGCT TCTGGAACTA CGCCCGCCCA 
CCCTCTGTCA TATTGGAGCC AGATGTGTTC CCTGGAAATT GCTGGGCTTT TGAAGGTGAG 
CAAGGCCAGG TGGTGATCCG ACTGCCAGGT CATGTGCAGT TAAGCGACAT CACCCTGCAG 
CACCCTCCAC CCACTGTGGC ACACACCGGA GGAGCCAGCA GTGCGCCCCG GGACTTCGCA 
GTCTTTGGGC TCCAAGCTGA TGATGACGAG ACTGAAGTGT TCTTGGGAAA ATTCATCTTT 
GAAGTGCAGA AATCTGAAAT TCAGACTTTC CACCTCCAGA ATGACCCTCC ATCTGCCTTC 
CCCAAGGTGA AGATTCAGAT TCTAAGCAAC TGGGGCCACC CACGCTTCAC ATGCTTATAT 
CGAGTCCGTG CCCATGGTGT GCGGATCTCA GAGTCGGCAG AGGATAATGC CATGGGGGTC 
ACAGGGGGAC CCCATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Spag4, a novel sperm protein, binds outer dense-fiber protein Odf1 and localizes to microtubules of manchette and axoneme.
Developmental biology211(1)109-23(1999 Jul)
Shao X,Tarnasky HA,Lee JP,Oko R,van der Hoorn FA


book

A novel testis-specific gene, SPAG4, whose product interacts specifically with outer dense fiber protein ODF27, maps to human chromosome 20q11.2.
Cytogenetics and cell genetics81(1)65-7(1998)
Tarnasky H,Gill D,Murthy S,Shao X,Demetrick DJ,van der Hoorn FA