ALF1 cDNA ORF clone, Saccharomyces cerevisiae S288C

  • Gene

  • Clones

  • gRNAs

The following ALF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ALF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi04490 NM_001182986.1
Latest version!
Saccharomyces cerevisiae S288C Alf1p (ALF1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSi04490
    Clone ID Related Accession (Same CDS sequence) NM_001182986.1
    Accession Version NM_001182986.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 765bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product Alf1p
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001146). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGGTTAGAG TTGTCATAGA GAGTGAATTG GTCCGTACAG AAAAGGAATT ACCAAATTCG 
    CTTAAATTAA GGCAGTTCAA GGACCGATTG TACCATGTTA CCGGAGTGGA ACCAGAGGAT
    ATGGAAATAG TTGTGAAAAG ACAGTACGAC AACAAGGAGA TATATAGTAC TAAAAAGGGG
    GGTGCCTATT CAAATGAGGA TGAAGATGCC AACTTCCTAA AAGGAGAGGA GGAGCTTATT
    GTGGTTGTTA CAGACTCTAA TGCCCAATCA ATCTCTAACC AGCTGGCCAC GCAAGCGGAA
    GGAATCCCCT CAATGGAAGT AATTAGCGAG GAGGATTATT TGCGAAGGGA TCAATCCGTA
    TTACGATGGA AGATGGCTCA CGGCTATGGT AGGTTTAATG CTGCACAGCA GAGCCAGCGC
    GCTGCGCTGG CAAAGCAGGA CGAAGCATAT GCCCGAGAGC AATTAACGGC GGCTATCGGC
    CGCCACTGTC GTGTCACAGT AGACGGGAGC GCTCCACGTG AGGCCATTTT ACGGTACGTA
    GGACCACTAC CGTTGGACGT GATGGGCACA TGGTGCGGTG TGGAATTCCC GGAAGCCGCA
    GGCAAAAACG ATGGGCGCAT AAATGGGGTA ACACTGTTTG GGCCTGTGGC GCCGGGTCAC
    GGATCGTTTG TGCGTCCGCG GGCTGTGGAA ATTCTATCAA AAGACGAAGA ATCCGCCGAG
    GTGGAAGACG TCCATGATGA CGTGGAGAGC GATGATGAAA TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_014251.1
    CDS1..765
    Translation

    Target ORF information:

    RefSeq Version NM_001182986.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C Alf1p (ALF1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001182986.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGGTTAGAG TTGTCATAGA GAGTGAATTG GTCCGTACAG AAAAGGAATT ACCAAATTCG 
    CTTAAATTAA GGCAGTTCAA GGACCGATTG TACCATGTTA CCGGAGTGGA ACCAGAGGAT
    ATGGAAATAG TTGTGAAAAG ACAGTACGAC AACAAGGAGA TATATAGTAC TAAAAAGGGG
    GGTGCCTATT CAAATGAGGA TGAAGATGCC AACTTCCTAA AAGGAGAGGA GGAGCTTATT
    GTGGTTGTTA CAGACTCTAA TGCCCAATCA ATCTCTAACC AGCTGGCCAC GCAAGCGGAA
    GGAATCCCCT CAATGGAAGT AATTAGCGAG GAGGATTATT TGCGAAGGGA TCAATCCGTA
    TTACGATGGA AGATGGCTCA CGGCTATGGT AGGTTTAATG CTGCACAGCA GAGCCAGCGC
    GCTGCGCTGG CAAAGCAGGA CGAAGCATAT GCCCGAGAGC AATTAACGGC GGCTATCGGC
    CGCCACTGTC GTGTCACAGT AGACGGGAGC GCTCCACGTG AGGCCATTTT ACGGTACGTA
    GGACCACTAC CGTTGGACGT GATGGGCACA TGGTGCGGTG TGGAATTCCC GGAAGCCGCA
    GGCAAAAACG ATGGGCGCAT AAATGGGGTA ACACTGTTTG GGCCTGTGGC GCCGGGTCAC
    GGATCGTTTG TGCGTCCGCG GGCTGTGGAA ATTCTATCAA AAGACGAAGA ATCCGCCGAG
    GTGGAAGACG TCCATGATGA CGTGGAGAGC GATGATGAAA TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
    Nature387(6632 Suppl)93-8(1997 May)
    Philippsen P,Kleine K,P?hlmann R,D?sterh?ft A,Hamberg K,Hegemann JH,Obermaier B,Urrestarazu LA,Aert R,Albermann K,Altmann R,Andr? B,Baladron V,Ballesta JP,B?cam AM,Beinhauer J,Boskovic J,Buitrago MJ,Bussereau F,Coster F,Crouzet M,D'Angelo M,Dal Pero F,De Antoni A,Del Rey F,Doignon F,Domdey H,Dubois E,Fiedler T,Fleig U,Floeth M,Fritz C,Gaillardin C,Garcia-Cantalejo JM,Glansdorff NN,Goffeau A,Gueldener U,Herbert C,Heumann K,Heuss-Neitzel D,Hilbert H,Hinni K,Iraqui Houssaini I,Jacquet M,Jimenez A,Jonniaux JL,Karpfinger L,Lanfranchi G,Lepingle A,Levesque H,Lyck R,Maftahi M,Mallet L,Maurer KC,Messenguy F,Mewes HW,M?sti D,Nasr F,Nicaud JM,Niedenthal RK,Pandolfo D,Pi?rard A,Piravandi E,Planta RJ,Pohl TM,Purnelle B,Rebischung C,Remacha M,Revuelta JL,Rinke M,Saiz JE,Sartorello F,Scherens B,Sen-Gupta M,Soler-Mira A,Urbanus JH,Valle G,Van Dyck L,Verhasselt P,Vierendeels F,Vissers S,Voet M,Volckaert G,Wach A,Wambutt R,Wedler H,Zollner A,Hani J


    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


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