• English
  • Sign In
    Please Sign In
    Sign In
    New to GenScript?
    Register
  • Contact Us
×
Contact us now: +1-877-436-7274

NIP100 cDNA ORF clone, Saccharomyces cerevisiae S288C

Summary

Gene Symbol NIP100
Entrez Gene ID 855929
Full Name Nip100p
Synonyms PAC13
Gene Type protein-coding
Organism Saccharomyces cerevisiae S288C

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001183988.1 NP_015151.1 Nip100p

Pathways from BioSystems


Gene Ontology


Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Bussey H, Storms RK, Ahmed A, Albermann K, Allen E, Ansorge W, Araujo R, Aparicio A, Barrell B, Badcock K, Benes V, Botstein D, Bowman S, Br?ckner M, Carpenter J, Cherry JM, Chung E, Churcher C, Coster F, Davis K, Davis RW, Dietrich FS, Delius H, DiPaolo T, Hani J
Nature387(6632 Suppl)103-5(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

interactions of a yeast ortholog of N-IC (N-Pac11) with yeast LC8 (Dyn2) or with the intermediate chain-binding subunit of yeast dynactin (Nip100).
Title: Interactions of Yeast Dynein with Dynein Light Chain and Dynactin: GENERAL IMPLICATIONS FOR INTRINSICALLY DISORDERED DUPLEX SCAFFOLDS IN MULTIPROTEIN ASSEMBLIES.

Data suggest that She1p restricts dynein-dependent spindle positioning to anaphase by inhibiting the association of dynein with the complete dynactin complex.
Title: Dynein-driven mitotic spindle positioning restricted to anaphase by She1p inhibition of dynactin recruitment.

Enhancement of dynein processivity by dynactin does not require the microtubule binding domains of Nip100
Title: Regulation of the processivity and intracellular localization of Saccharomyces cerevisiae dynein by dynactin.

Dynein/dynactin helps to generate a spindle pole separating force in Saccharomyces cerevisiae.
Title: The Saccharomyces cerevisiae homolog of p24 is essential for maintaining the association of p150Glued with the dynactin complex.

The following NIP100 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NIP100 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi04800 NM_001183988.1
Latest version!		 Saccharomyces cerevisiae S288C Nip100p (NIP100), partial mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $510.30
$729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OSi04800
Clone ID Related Accession (Same CDS sequence) NM_001183988.1
Accession Version NM_001183988.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2607bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-10-31
Organism Saccharomyces cerevisiae S288C
Product Nip100p
Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001148). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
ATGCGCAATG CAGGTGTTCA AGTTGACACA AATATGCAGA AGATAAGCTT ACAGGACACA 
GTTCTAGTCA ACGAGATGAA GGGGCGGGTG AAATTCATTG GGGAAACTCA ATTTGCAAAA 
GGCATATGGT ACGGCATTGA ACTAGATAAA CCGTTAGGAA AGAATGATGG AAGCGCTAAC 
GGTATTCGTT ACTTTGATAT CGACCTGAAG AAAGCTAATT CAAATGGTGG TTATTATGGT 
CTTTTCTGCA AGAAAGATAC GTTGCAGTTT TATAAACCAG ATGACGATGA GCATAGTTTG 
CTAAATGGTA ATGCAGCGCA AGAAACAATT AAGAATCTAC AAGTAAAATG TGAATCATTG 
GCGTCCAAAT TAAATAAAAT TAAAATAGAA AATCATGAGC TGAAAACTTC CGTTGAAAAG 
CTGAGTACTA ATGAAACTGT TCTATTGTCT AAAATCTCAA GATTGGATAA ACTAGTCAAA 
GAACTGAAAG TGGAAAATGG TAATATGAAA ACTCATTTGG ATAATTTCAA TCACCTATTA 
GATGCTTCAG ATAGTGTAAT GGCACCGGAT CTCGACAAAG GCACACTGTT AGAAAGGAGC 
CATCTTCTTC AGGGACTACT CGACCAAACA AAGTTGTCAT ATGATAAAGC TATGAAGGTG 
CAAGAGGACC TGCTCGAGGA GAATACTCAG TTATTAGAAG AAAATGCAGT ATTATCTAAA 
AAAATATCGG ATTTAGGTCT GCAATTGCAA CAGACTAATA ATACAATCGG CGACCTTGCC 
TTGCAGATTG AAGCTCAAAG TAAATCATCT AATATTGTTG ATAAATTGAC TAATGATAAT 
ATCCTACTCA CAAGTAATAT AAAGGCTTTA AACAATGAGT TGGAGGAACT ACAGGCAAAA 
GAAAAATTGG ATGAAAATTT AAGAATTACC TATGAGCAAC TCGAGCAAGA ACTGAGACTG 
CAATTAAGTA ATTTGCAATC TGCATTGGAA AATGAAAAGG AGATAGCAGG CACATATATC 
GAAGAAAATT CAAGATTAAA AGCAACATTG GAATCTATTG AAGCCAAAAC ATCTCACAAA 
TTTCAAAGTC TTGAACTAAA AGTGAACACT TTACAGGAGG AATTATATCA AAATAAATTG 
CTAAAGAAAT TTTACCAAAT ATATGAGCCA TTTGCACAAC CACATTTGGC GGCTCTTTCC 
TCTCAATTGC AATATTTAGC CGAAGTAATC GAAAGTGAAA ATTTTGGAAA GTTGGAGAAT 
ATTGAAATCC ATATAATTCT AAAAGTGTTG AGCTCTATCA GTTATGCGCT TCATATTTAC 
ACCATAAAGA ATACTCCCGA TCACCTGGAA ACGACATTAC AATGTTTCAA AGTAAATATT 
GCTCCGATTT CGATGTGGCT AAGCGAGTTT TTGCAAAGGA AGTTTTCTTC TAAACAGGAA 
ACCGCCTTTT CCATTTGTCA GTTTTTAGAA GATAATAAGT TTCTAGATAA GGATGTGACC 
TTGATACTAA AAATACTTCA TCCGATATTG GAAACAACTG TTCCGAAGCT GTTGGCTTTT 
CTTAGAACGA ACAGCAATTT CAATGACAAT GATACCCTAT GTCTTATTGG TTCCCTTTAC 
GAGAGATCAC TTTCACTTAT TGCCAGAATA GATAAACTTA TCGGAAAGGA AGAAATTTCG 
AAACAAGACA ATAGGTTATT TTTATATCCT TCCTGTGATA TAACCCTGTC CTCTATCTTA 
ACGATACTTT TTTCAGATGC ACTGTTTTTG CGACAAGATT ACAAACGTAT TTCTTCTTTG 
AAAAAACTAG AGGTATTTTT TCAAGGAATA GAAAGTCTCT TAGAAAACAT AACTATATTT 
CCTGAGCAAC CCAGTCAACA AACAAGTGAT TCAGAAAGTC AATGTAATAT TAAGGAGGGC 
AATTTTTCAA ACTCGTTATT AAGCGATCGC TTGAATGAAG AGAACATTCG ACTCAAAGAA 
GTACTGGTCC AAAAGGAAAA TATGCTAACT GAATTGGAAA CAAAAATTAA AATTATTATT 
GGGAGGGATT TAGAGAGGAA AACCCTTGAA GAGAATATCA AAACACTCAA AGTAGAGTTA 
AATAACAAAA ATGAGGAAAA TTGTGGCAAA ACAGAAATTC TTAACAAGCT GAAAGAAGAA 
AATTTCAACC TAGTAAACCG TTTGAAGAAT ATGGAGCTCA AACTATATCA AATAAAGGAT 
AATAATACGT TGAACAAAAT TTATTTAGAC AGAGAGAAAG TAGATAGAGT AAATTTAGTT 
TCGGAAATCA TGGAATTGAG AGAAACCATA AGACGACAAA TAAAAGAGCA GAAACGAGTG 
AGCATCGATT TCAGCTGGTT AGACGAGCTT CCAGCTGTGG AAAATAAGCA ACCATTCAAA 
GAACATATCA ACCACAGTTT GGACACCTTG GGGATAGAAA TGTTCAACTT CGTGAGTACC 
TCACGGATTT TAGATTTAAA GCTTGATCAA CCTTTGGCAG AAGATGAACT CTGGCATGAA 
AGAGATCATT CTTACATTTC GTATTTAAAG CGCAAGAGGA AAAATATACG ACTCAAATCT 
CAAAATGTTG TTACATATTA CAAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_015151.1
CDS1..2607
Translation

Target ORF information:

RefSeq Version NM_001183988.1
Organism Saccharomyces cerevisiae S288C
Definition Saccharomyces cerevisiae S288C Nip100p (NIP100), partial mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001183988.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
ATGCGCAATG CAGGTGTTCA AGTTGACACA AATATGCAGA AGATAAGCTT ACAGGACACA 
GTTCTAGTCA ACGAGATGAA GGGGCGGGTG AAATTCATTG GGGAAACTCA ATTTGCAAAA 
GGCATATGGT ACGGCATTGA ACTAGATAAA CCGTTAGGAA AGAATGATGG AAGCGCTAAC 
GGTATTCGTT ACTTTGATAT CGACCTGAAG AAAGCTAATT CAAATGGTGG TTATTATGGT 
CTTTTCTGCA AGAAAGATAC GTTGCAGTTT TATAAACCAG ATGACGATGA GCATAGTTTG 
CTAAATGGTA ATGCAGCGCA AGAAACAATT AAGAATCTAC AAGTAAAATG TGAATCATTG 
GCGTCCAAAT TAAATAAAAT TAAAATAGAA AATCATGAGC TGAAAACTTC CGTTGAAAAG 
CTGAGTACTA ATGAAACTGT TCTATTGTCT AAAATCTCAA GATTGGATAA ACTAGTCAAA 
GAACTGAAAG TGGAAAATGG TAATATGAAA ACTCATTTGG ATAATTTCAA TCACCTATTA 
GATGCTTCAG ATAGTGTAAT GGCACCGGAT CTCGACAAAG GCACACTGTT AGAAAGGAGC 
CATCTTCTTC AGGGACTACT CGACCAAACA AAGTTGTCAT ATGATAAAGC TATGAAGGTG 
CAAGAGGACC TGCTCGAGGA GAATACTCAG TTATTAGAAG AAAATGCAGT ATTATCTAAA 
AAAATATCGG ATTTAGGTCT GCAATTGCAA CAGACTAATA ATACAATCGG CGACCTTGCC 
TTGCAGATTG AAGCTCAAAG TAAATCATCT AATATTGTTG ATAAATTGAC TAATGATAAT 
ATCCTACTCA CAAGTAATAT AAAGGCTTTA AACAATGAGT TGGAGGAACT ACAGGCAAAA 
GAAAAATTGG ATGAAAATTT AAGAATTACC TATGAGCAAC TCGAGCAAGA ACTGAGACTG 
CAATTAAGTA ATTTGCAATC TGCATTGGAA AATGAAAAGG AGATAGCAGG CACATATATC 
GAAGAAAATT CAAGATTAAA AGCAACATTG GAATCTATTG AAGCCAAAAC ATCTCACAAA 
TTTCAAAGTC TTGAACTAAA AGTGAACACT TTACAGGAGG AATTATATCA AAATAAATTG 
CTAAAGAAAT TTTACCAAAT ATATGAGCCA TTTGCACAAC CACATTTGGC GGCTCTTTCC 
TCTCAATTGC AATATTTAGC CGAAGTAATC GAAAGTGAAA ATTTTGGAAA GTTGGAGAAT 
ATTGAAATCC ATATAATTCT AAAAGTGTTG AGCTCTATCA GTTATGCGCT TCATATTTAC 
ACCATAAAGA ATACTCCCGA TCACCTGGAA ACGACATTAC AATGTTTCAA AGTAAATATT 
GCTCCGATTT CGATGTGGCT AAGCGAGTTT TTGCAAAGGA AGTTTTCTTC TAAACAGGAA 
ACCGCCTTTT CCATTTGTCA GTTTTTAGAA GATAATAAGT TTCTAGATAA GGATGTGACC 
TTGATACTAA AAATACTTCA TCCGATATTG GAAACAACTG TTCCGAAGCT GTTGGCTTTT 
CTTAGAACGA ACAGCAATTT CAATGACAAT GATACCCTAT GTCTTATTGG TTCCCTTTAC 
GAGAGATCAC TTTCACTTAT TGCCAGAATA GATAAACTTA TCGGAAAGGA AGAAATTTCG 
AAACAAGACA ATAGGTTATT TTTATATCCT TCCTGTGATA TAACCCTGTC CTCTATCTTA 
ACGATACTTT TTTCAGATGC ACTGTTTTTG CGACAAGATT ACAAACGTAT TTCTTCTTTG 
AAAAAACTAG AGGTATTTTT TCAAGGAATA GAAAGTCTCT TAGAAAACAT AACTATATTT 
CCTGAGCAAC CCAGTCAACA AACAAGTGAT TCAGAAAGTC AATGTAATAT TAAGGAGGGC 
AATTTTTCAA ACTCGTTATT AAGCGATCGC TTGAATGAAG AGAACATTCG ACTCAAAGAA 
GTACTGGTCC AAAAGGAAAA TATGCTAACT GAATTGGAAA CAAAAATTAA AATTATTATT 
GGGAGGGATT TAGAGAGGAA AACCCTTGAA GAGAATATCA AAACACTCAA AGTAGAGTTA 
AATAACAAAA ATGAGGAAAA TTGTGGCAAA ACAGAAATTC TTAACAAGCT GAAAGAAGAA 
AATTTCAACC TAGTAAACCG TTTGAAGAAT ATGGAGCTCA AACTATATCA AATAAAGGAT 
AATAATACGT TGAACAAAAT TTATTTAGAC AGAGAGAAAG TAGATAGAGT AAATTTAGTT 
TCGGAAATCA TGGAATTGAG AGAAACCATA AGACGACAAA TAAAAGAGCA GAAACGAGTG 
AGCATCGATT TCAGCTGGTT AGACGAGCTT CCAGCTGTGG AAAATAAGCA ACCATTCAAA 
GAACATATCA ACCACAGTTT GGACACCTTG GGGATAGAAA TGTTCAACTT CGTGAGTACC 
TCACGGATTT TAGATTTAAA GCTTGATCAA CCTTTGGCAG AAGATGAACT CTGGCATGAA 
AGAGATCATT CTTACATTTC GTATTTAAAG CGCAAGAGGA AAAATATACG ACTCAAATCT 
CAAAATGTTG TTACATATTA CAAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Nature387(6632 Suppl)103-5(1997 May)
Bussey H,Storms RK,Ahmed A,Albermann K,Allen E,Ansorge W,Araujo R,Aparicio A,Barrell B,Badcock K,Benes V,Botstein D,Bowman S,Br?ckner M,Carpenter J,Cherry JM,Chung E,Churcher C,Coster F,Davis K,Davis RW,Dietrich FS,Delius H,DiPaolo T,Hani J


book

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG