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RAT1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Summary

Gene Symbol RAT1
Entrez Gene ID 854213
Full Name ssRNA exonuclease RAT1
Synonyms HKE1,TAP1,XRN2
Gene Type protein-coding
Organism Saccharomyces cerevisiae S288C

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001183467.1 NP_014691.1 ssRNA exonuclease RAT1

Pathways from BioSystems


Homologies


Eremothecium gossypii AGOS_AGR303W NP_986969.2
Pan troglodytes (chimpanzee) XRN2 XP_514546.2
Canis lupus familiaris (dog) XRN2 XP_534324.2
Rattus norvegicus (Norway rat) Xrn2 NP_001102066.1
Drosophila melanogaster (fruit fly) Rat1 NP_001260166.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP004090 XP_309641.5
Caenorhabditis elegans (roundworm) xrn-2 NP_496958.2
Saccharomyces cerevisiae (baker's yeast) RAT1 NP_014691.1
Xenopus tropicalis (tropical clawed frog) xrn2 XP_002939479.1
Homo sapiens (human) XRN2 NP_036387.2
Bos taurus (cattle) XRN2 NP_001179401.1
Mus musculus (house mouse) Xrn2 NP_036047.2
Schizosaccharomyces pombe (fission yeast) dhp1 NP_594155.1
Neurospora crassa NCU01643 XP_956265.1
XRN2 XP_001094734.2
Gallus gallus (chicken) XRN2 NP_001026204.1
Danio rerio (zebrafish) xrn2 NP_001001944.2
Magnaporthe oryzae (rice blast fungus) MGG_11426 XP_003717803.1
Kluyveromyces lactis KLLA0F07469g XP_455419.1
Arabidopsis thaliana (thale cress) XRN3 NP_565114.1
Oryza sativa (rice) Os01g0872700 NP_001044947.1

Gene Ontology


Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
Dujon B, Albermann K, Aldea M, Alexandraki D, Ansorge W, Arino J, Benes V, Bohn C, Bolotin-Fukuhara M, Bordonn? R, Boyer J, Camasses A, Casamayor A, Casas C, Ch?ret G, Cziepluch C, Daignan-Fornier B, Dang DV, de Haan M, Delius H, Durand P, Fairhead C, Feldmann H, Gaillon L, Kleine K
Nature387(6632 Suppl)98-102(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results indicate that multiple mechanistic features contribute to 5'-3' exoribonuclease Rat1-mediated termination of RNA polymerase II (RNAPII).
Title: Unraveling the mechanistic features of RNA polymerase II termination by the 5'-3' exoribonuclease Rat1.

Data indicate that Rat1 exonuclease (Rat1) acts catalytically in 5.8S and 25S rRNA processing but binds to the pre-rRNA prior to these activities.
Title: Kinetic analysis demonstrates a requirement for the Rat1 exonuclease in cotranscriptional pre-rRNA cleavage.

Degradation of the nascent transcript allows Rat1 to pursue Pol II in a guided fashion and arrive at the site of RNA exit from Pol II.
Title: Dismantling promoter-driven RNA polymerase II transcription complexes in vitro by the termination factor Rat1.

Rat1 exonuclease pre-rRNA folding and 5.8S rRNA maturation are regulated by a cluster of ribosome synthesis factors
Title: A cluster of ribosome synthesis factors regulate pre-rRNA folding and 5.8S rRNA maturation by the Rat1 exonuclease.

data suggest that Rat1p serves to terminate RNAPII molecules engaged in the production of uncapped RNA, regardless of their position on the gene locus.
Title: The yeast 5'-3' exonuclease Rat1p functions during transcription elongation by RNA polymerase II.

The following RAT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi03359 NM_001183467.1
Latest version!		 Saccharomyces cerevisiae S288C ssRNA exonuclease RAT1 (RAT1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OSi03359
Clone ID Related Accession (Same CDS sequence) NM_001183467.1
Accession Version NM_001183467.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3021bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-10-31
Organism Saccharomyces cerevisiae S288C
Product ssRNA exonuclease RAT1
Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001147). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
ATGGGTGTTC CGTCATTTTT CAGATGGCTA TCTCGAAAAT ATCCAAAGAT CATATCCCCA 
GTATTGGAAG AGCAACCTCA GATAGTCGAC GGTGTCATAT TGCCGTTGGA TTATTCCGCC 
TCAAATCCAA ACGGTGAGTT AGACAACTTG TATTTGGATA TGAATGGTAT TGTTCATCCA 
TGTTCGCACC CAGAAAACAA ACCGCCTCCA GAGACTGAAG ATGAAATGCT TTTGGCAGTT 
TTTGAATATA CCAATCGTGT GTTAAATATG GCTAGACCAC GCAAGGTACT TGTTATGGCT 
GTTGATGGTG TTGCTCCTCG TGCCAAAATG AACCAGCAGA GAGCGCGTAG ATTTAGGAGT 
GCTAGGGATG CTCAGATTGA AAACGAAGCC AGGGAAGAAA TTATGAGACA GCGAGAAGAA 
GTTGGTGAGA TAATCGATGA TGCCGTCAGG AACAAGAAAA CTTGGGATTC AAATGCGATC 
ACTCCAGGCA CCCCATTTAT GGATAAGCTT GCCGCGGCCC TCCGTTATTG GACAGCATTT 
AAACTGGCCA CTGACCCTGG TTGGAAAAAC CTGCAGGTGA TTATCAGTGA CGCCACCGTC 
CCAGGTGAAG GTGAGCACAA AATAATGAAT TTCATTAGGT CTCAAAGAGC TGATCCTGAA 
TATAATCCCA ACACAACGCA CTGTATATAC GGTTTGGATG CTGATTTAAT TTTTTTGGGC 
CTTGCCACTC ATGAACCACA TTTTAAAATT CTAAGAGAAG ATGTTTTTGC TCAAGACAAT 
CGGAAAAGAA ATAACTTAAA AGATACTATA AATATGACTG AAGAAGAAAA ACAATTTCTC 
CAAAAACAAA ATTCCGAACA ACCTTTCTTG TGGCTGCACA TAAATGTTTT GAGGGAATAC 
TTGTCCGCTG AGTTATGGGT TCCGGGTCTT CCATTTACAT TTGATTTAGA AAGGGCTATC 
GACGATTGGG TTTTCATGTG CTTTTTCTGT GGTAATGACT TTTTACCACA TTTACCATGC 
TTAGATGTCA GAGAAAATAG TATTGATATT CTCTTGGATA TTTGGAAGGT AGTACTACCG 
AAATTGAAAA CGTACATGAC CTGCGACGGT GTCTTGAACC TCCCTTCTGT TGAAACGCTA 
TTGCAACACC TTGGCTCCCG TGAGGGGGAT ATCTTCAAAA CTAGACATAT TCAAGAAGCC 
AGAAAGAAAG AAGCTTTTGA AAGAAGGAAA GCGCAGAAAA ATATGTCAAA AGGTCAAGAC 
AGGCATCCTA CAGTAGCTAC GGAGCAACTG CAAATGTATG ACACACAAGG AAATTTAGCA 
AAAGGTTCAT GGAACTTGAC CACCAGTGAT ATGGTAAGGT TGAAAAAGGA ACTGATGCTT 
GCGAACGAGG GGAACGAAGA AGCAATCGCC AAAGTTAAGC AACAGAGTGA TAAGAACAAT 
GAACTGATGA AAGATATCAG CAAGGAAGAA ATTGATGATG CTGTTAGTAA GGCTAACAAA 
ACTAATTTTA ACCTTGCAGA AGTTATGAAA CAGAAAATAA TAAATAAGAA ACACCGTCTT 
GAAAAGGATA ATGAAGAGGA AGAAATTGCT AAGGATAGCA AAAAAGTTAA GACAGAGAAA 
GCGGAATCAG AGTGCGATCT CGATGCAGAG ATCAAAGATG AGATTGTGGC TGACGTAAAC 
GACAGGGAAA ACTCTGAAAC TACAGAAGTA TCAAGAGATT CACCAGTCCA CAGCACAGTG 
AATGTCAGCG AAGGTCCCAA AAATGGGGTC TTTGATACAG ATGAATTTGT CAAACTATTC 
GAACCTGGTT ACCACGAAAG ATATTACACA GCTAAATTTC ACGTTACTCC TCAAGATATT 
GAACAGTTAA GAAAAGATAT GGTAAAATGC TATATCGAAG GGGTCGCCTG GGTACTAATG 
TATTACTATC AAGGTTGTGC CTCTTGGAAT TGGTTCTATC CGTACCATTA TGCTCCTCTG 
GCAACAGATT TCCATGGGTT TTCTCACTTG GAGATAAAGT TCGAAGAAGG TACTCCATTT 
TTACCTTACG AACAATTGAT GAGTGTTTTA CCAGCTGCAT CTGGCCATGC TTTGCCAAAA 
ATTTTCCGGT CTTTGATGTC TGAACCTGAC TCTGAAATCA TTGATTTTTA TCCTGAAGAA 
TTTCCCATAG ATATGAATGG TAAGAAGATG TCCTGGCAAG GCATAGCGTT GCTACCATTC 
ATCGATCAGG ATAGACTGTT AACAGCTGTT CGTGCACAAT ATCCTTTACT TTCTGATGCT 
GAGAGGGCAA GAAATATTCG CGGAGAACCT GTTTTATTAA TAAGTAACAA GAATGCTAAT 
TATGAGAGGT TTTCGAAGAA ATTATACTCA AAGGAAAACA ACAACAACAA TGTTGTTGTG 
AAGTTTCAAC ACTTCAAGAG TGGTTTAAGT GGTATTGTAT CAAAGGACGT TGAAGGTTTC 
GAATTAAATG GGAAAATTGT ATGTCCGATT CAAGGTGGGT CACTGCCAAA TTTATCTACC 
ACCTTGATTT TAAAGATGTC CTATAGATTA ATTCCGTTGC CATCGAGAAA TAAGTCTATT 
ATACTGAACG GGTTCATTCC GTCAGAACCG GTACTAACAG CATATGACCT TGATTCCATA 
ATGTACAAGT ATAATAACCA AAATTACTCC CGTCGCTGGA ACTTTGGTAA TGATTTGAAG 
CAAAATATTG TTCCAGTAGG ACCAAAGGGT ATTACTCAGT ACAAACCAAG AACTGGTGGC 
TATCGAGCAT TCTTTTATTT TGCTGAGCTC AGTAGAAATA ATGTCCAACC CGCCCACAAC 
TATGGTAGGA ATAGCTACAA CAGTCAACCC GGCTTCAACA ATAGCAGGTA TGATGGTGGA 
AACAATAATT ACAGACAAAA TTCAAATTAC AGGAACAATA ACTATTCCGG AAATAGAAAT 
AGTGGACAGT ACAGCGGCAA CAGCTACTCT CGGAATAACA AGCAAAGTCG GTATGACAAT 
TCAAGAGCAA ATAGGCGTTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_014691.1
CDS1..3021
Translation

Target ORF information:

RefSeq Version NM_001183467.1
Organism Saccharomyces cerevisiae S288C
Definition Saccharomyces cerevisiae S288C ssRNA exonuclease RAT1 (RAT1), partial mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001183467.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
ATGGGTGTTC CGTCATTTTT CAGATGGCTA TCTCGAAAAT ATCCAAAGAT CATATCCCCA 
GTATTGGAAG AGCAACCTCA GATAGTCGAC GGTGTCATAT TGCCGTTGGA TTATTCCGCC 
TCAAATCCAA ACGGTGAGTT AGACAACTTG TATTTGGATA TGAATGGTAT TGTTCATCCA 
TGTTCGCACC CAGAAAACAA ACCGCCTCCA GAGACTGAAG ATGAAATGCT TTTGGCAGTT 
TTTGAATATA CCAATCGTGT GTTAAATATG GCTAGACCAC GCAAGGTACT TGTTATGGCT 
GTTGATGGTG TTGCTCCTCG TGCCAAAATG AACCAGCAGA GAGCGCGTAG ATTTAGGAGT 
GCTAGGGATG CTCAGATTGA AAACGAAGCC AGGGAAGAAA TTATGAGACA GCGAGAAGAA 
GTTGGTGAGA TAATCGATGA TGCCGTCAGG AACAAGAAAA CTTGGGATTC AAATGCGATC 
ACTCCAGGCA CCCCATTTAT GGATAAGCTT GCCGCGGCCC TCCGTTATTG GACAGCATTT 
AAACTGGCCA CTGACCCTGG TTGGAAAAAC CTGCAGGTGA TTATCAGTGA CGCCACCGTC 
CCAGGTGAAG GTGAGCACAA AATAATGAAT TTCATTAGGT CTCAAAGAGC TGATCCTGAA 
TATAATCCCA ACACAACGCA CTGTATATAC GGTTTGGATG CTGATTTAAT TTTTTTGGGC 
CTTGCCACTC ATGAACCACA TTTTAAAATT CTAAGAGAAG ATGTTTTTGC TCAAGACAAT 
CGGAAAAGAA ATAACTTAAA AGATACTATA AATATGACTG AAGAAGAAAA ACAATTTCTC 
CAAAAACAAA ATTCCGAACA ACCTTTCTTG TGGCTGCACA TAAATGTTTT GAGGGAATAC 
TTGTCCGCTG AGTTATGGGT TCCGGGTCTT CCATTTACAT TTGATTTAGA AAGGGCTATC 
GACGATTGGG TTTTCATGTG CTTTTTCTGT GGTAATGACT TTTTACCACA TTTACCATGC 
TTAGATGTCA GAGAAAATAG TATTGATATT CTCTTGGATA TTTGGAAGGT AGTACTACCG 
AAATTGAAAA CGTACATGAC CTGCGACGGT GTCTTGAACC TCCCTTCTGT TGAAACGCTA 
TTGCAACACC TTGGCTCCCG TGAGGGGGAT ATCTTCAAAA CTAGACATAT TCAAGAAGCC 
AGAAAGAAAG AAGCTTTTGA AAGAAGGAAA GCGCAGAAAA ATATGTCAAA AGGTCAAGAC 
AGGCATCCTA CAGTAGCTAC GGAGCAACTG CAAATGTATG ACACACAAGG AAATTTAGCA 
AAAGGTTCAT GGAACTTGAC CACCAGTGAT ATGGTAAGGT TGAAAAAGGA ACTGATGCTT 
GCGAACGAGG GGAACGAAGA AGCAATCGCC AAAGTTAAGC AACAGAGTGA TAAGAACAAT 
GAACTGATGA AAGATATCAG CAAGGAAGAA ATTGATGATG CTGTTAGTAA GGCTAACAAA 
ACTAATTTTA ACCTTGCAGA AGTTATGAAA CAGAAAATAA TAAATAAGAA ACACCGTCTT 
GAAAAGGATA ATGAAGAGGA AGAAATTGCT AAGGATAGCA AAAAAGTTAA GACAGAGAAA 
GCGGAATCAG AGTGCGATCT CGATGCAGAG ATCAAAGATG AGATTGTGGC TGACGTAAAC 
GACAGGGAAA ACTCTGAAAC TACAGAAGTA TCAAGAGATT CACCAGTCCA CAGCACAGTG 
AATGTCAGCG AAGGTCCCAA AAATGGGGTC TTTGATACAG ATGAATTTGT CAAACTATTC 
GAACCTGGTT ACCACGAAAG ATATTACACA GCTAAATTTC ACGTTACTCC TCAAGATATT 
GAACAGTTAA GAAAAGATAT GGTAAAATGC TATATCGAAG GGGTCGCCTG GGTACTAATG 
TATTACTATC AAGGTTGTGC CTCTTGGAAT TGGTTCTATC CGTACCATTA TGCTCCTCTG 
GCAACAGATT TCCATGGGTT TTCTCACTTG GAGATAAAGT TCGAAGAAGG TACTCCATTT 
TTACCTTACG AACAATTGAT GAGTGTTTTA CCAGCTGCAT CTGGCCATGC TTTGCCAAAA 
ATTTTCCGGT CTTTGATGTC TGAACCTGAC TCTGAAATCA TTGATTTTTA TCCTGAAGAA 
TTTCCCATAG ATATGAATGG TAAGAAGATG TCCTGGCAAG GCATAGCGTT GCTACCATTC 
ATCGATCAGG ATAGACTGTT AACAGCTGTT CGTGCACAAT ATCCTTTACT TTCTGATGCT 
GAGAGGGCAA GAAATATTCG CGGAGAACCT GTTTTATTAA TAAGTAACAA GAATGCTAAT 
TATGAGAGGT TTTCGAAGAA ATTATACTCA AAGGAAAACA ACAACAACAA TGTTGTTGTG 
AAGTTTCAAC ACTTCAAGAG TGGTTTAAGT GGTATTGTAT CAAAGGACGT TGAAGGTTTC 
GAATTAAATG GGAAAATTGT ATGTCCGATT CAAGGTGGGT CACTGCCAAA TTTATCTACC 
ACCTTGATTT TAAAGATGTC CTATAGATTA ATTCCGTTGC CATCGAGAAA TAAGTCTATT 
ATACTGAACG GGTTCATTCC GTCAGAACCG GTACTAACAG CATATGACCT TGATTCCATA 
ATGTACAAGT ATAATAACCA AAATTACTCC CGTCGCTGGA ACTTTGGTAA TGATTTGAAG 
CAAAATATTG TTCCAGTAGG ACCAAAGGGT ATTACTCAGT ACAAACCAAG AACTGGTGGC 
TATCGAGCAT TCTTTTATTT TGCTGAGCTC AGTAGAAATA ATGTCCAACC CGCCCACAAC 
TATGGTAGGA ATAGCTACAA CAGTCAACCC GGCTTCAACA ATAGCAGGTA TGATGGTGGA 
AACAATAATT ACAGACAAAA TTCAAATTAC AGGAACAATA ACTATTCCGG AAATAGAAAT 
AGTGGACAGT ACAGCGGCAA CAGCTACTCT CGGAATAACA AGCAAAGTCG GTATGACAAT 
TCAAGAGCAA ATAGGCGTTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
Nature387(6632 Suppl)98-102(1997 May)
Dujon B,Albermann K,Aldea M,Alexandraki D,Ansorge W,Arino J,Benes V,Bohn C,Bolotin-Fukuhara M,Bordonn? R,Boyer J,Camasses A,Casamayor A,Casas C,Ch?ret G,Cziepluch C,Daignan-Fornier B,Dang DV,de Haan M,Delius H,Durand P,Fairhead C,Feldmann H,Gaillon L,Kleine K


book

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG