THI12 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following THI12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the THI12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi04322 NM_001183170.1
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Saccharomyces cerevisiae S288C 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase (THI12), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSi04322
    Clone ID Related Accession (Same CDS sequence) NM_001183170.1
    Accession Version NM_001183170.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1023bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001146). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTCTACAG ACAAGATCAC ATTTTTGTTG AACTGGCAAC CAACCCCATA CCATATTCCA 
    ATTTTCTTGG CTCAAACCAA AGGTTACTTC AAGGAGCAAG GTCTAGACAT GGCCATCCTA
    GAACCAACCA ATCCTTCCGA TGTCACTGAG TTAATTGGAT CTGGTAAGGT CGACATGGGT
    TTGAAAGCCA TGATTCACAC CTTGGCTGCC AAGGCCCGTG GTTTCCCAGT GACCTCTGTT
    GCCTCTTTGT TGGACGAACC ATTTACCGGT GTCTTGTACT TAAAGGGCAG TGGTATCACT
    GAAGACTTCC AGTCCCTAAA GGGTAAGAAG ATCGGTTACG TTGGTGAATT CGGTAAGATC
    CAAATCGATG AATTGACCAA GCACTACGGT ATGAAGCCAG AAGACTACAC CGCAGTCAGA
    TGTGGTATGA ATGTCGCCAA GTACATCATC GAAGGTAAGA TTGATGCTGG TATTGGTATC
    GAATGTATGC AACAAGTCGA ATTGGAAGAG TACTTGGCTA AGCAAGGCAG ACCAGCTTCT
    GATGCTAAGA TGTTGAGAAT TGACAAGTTG GCTTGCTTGG GTTGCTGTTG CTTCTGTACC
    GTTCTTTACA TCTGCAACGA TGAATTTTTG AAGAAGAACC CTGAAAAGGT CAGAAAGTTC
    TTGAAAGCCA TCAAGAAGGC AACCGACTAC GTTCTAGCCG ACCCTGTGAA GGCTTGGAAA
    GAATACATCG ACTTCAAGCC TCGATTGAAC AACGATCTAT CTTACAAGCA ATACCAAAGA
    TGTTACGCTT ACTTCTCTTC ATCTTTGTAC AATGTTCACC GTGACTGGAA GAAGGTTACC
    GGTTACGGTA AGAGATTAGC CATCTTGCCA CCAGACTATG TCTCGAACTA CACTAATGAA
    TACTTGTCCT GGCCAGAACC AGAAGAGGTT TCTGATCCTT TGGAAGCTCA AAGATTGATG
    GCTATTCATC AAGAAAAATG CAGACAGGAA GGTACTTTCA AGAGATTGGC TCTTCCAGCT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_014067.1
    CDS1..1023
    Translation

    Target ORF information:

    RefSeq Version NM_001183170.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase (THI12), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001183170.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTCTACAG ACAAGATCAC ATTTTTGTTG AACTGGCAAC CAACCCCATA CCATATTCCA 
    ATTTTCTTGG CTCAAACCAA AGGTTACTTC AAGGAGCAAG GTCTAGACAT GGCCATCCTA
    GAACCAACCA ATCCTTCCGA TGTCACTGAG TTAATTGGAT CTGGTAAGGT CGACATGGGT
    TTGAAAGCCA TGATTCACAC CTTGGCTGCC AAGGCCCGTG GTTTCCCAGT GACCTCTGTT
    GCCTCTTTGT TGGACGAACC ATTTACCGGT GTCTTGTACT TAAAGGGCAG TGGTATCACT
    GAAGACTTCC AGTCCCTAAA GGGTAAGAAG ATCGGTTACG TTGGTGAATT CGGTAAGATC
    CAAATCGATG AATTGACCAA GCACTACGGT ATGAAGCCAG AAGACTACAC CGCAGTCAGA
    TGTGGTATGA ATGTCGCCAA GTACATCATC GAAGGTAAGA TTGATGCTGG TATTGGTATC
    GAATGTATGC AACAAGTCGA ATTGGAAGAG TACTTGGCTA AGCAAGGCAG ACCAGCTTCT
    GATGCTAAGA TGTTGAGAAT TGACAAGTTG GCTTGCTTGG GTTGCTGTTG CTTCTGTACC
    GTTCTTTACA TCTGCAACGA TGAATTTTTG AAGAAGAACC CTGAAAAGGT CAGAAAGTTC
    TTGAAAGCCA TCAAGAAGGC AACCGACTAC GTTCTAGCCG ACCCTGTGAA GGCTTGGAAA
    GAATACATCG ACTTCAAGCC TCGATTGAAC AACGATCTAT CTTACAAGCA ATACCAAAGA
    TGTTACGCTT ACTTCTCTTC ATCTTTGTAC AATGTTCACC GTGACTGGAA GAAGGTTACC
    GGTTACGGTA AGAGATTAGC CATCTTGCCA CCAGACTATG TCTCGAACTA CACTAATGAA
    TACTTGTCCT GGCCAGAACC AGAAGAGGTT TCTGATCCTT TGGAAGCTCA AAGATTGATG
    GCTATTCATC AAGAAAAATG CAGACAGGAA GGTACTTTCA AGAGATTGGC TCTTCCAGCT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
    Nature387(6632 Suppl)93-8(1997 May)
    Philippsen P,Kleine K,P?hlmann R,D?sterh?ft A,Hamberg K,Hegemann JH,Obermaier B,Urrestarazu LA,Aert R,Albermann K,Altmann R,Andr? B,Baladron V,Ballesta JP,B?cam AM,Beinhauer J,Boskovic J,Buitrago MJ,Bussereau F,Coster F,Crouzet M,D'Angelo M,Dal Pero F,De Antoni A,Del Rey F,Doignon F,Domdey H,Dubois E,Fiedler T,Fleig U,Floeth M,Fritz C,Gaillardin C,Garcia-Cantalejo JM,Glansdorff NN,Goffeau A,Gueldener U,Herbert C,Heumann K,Heuss-Neitzel D,Hilbert H,Hinni K,Iraqui Houssaini I,Jacquet M,Jimenez A,Jonniaux JL,Karpfinger L,Lanfranchi G,Lepingle A,Levesque H,Lyck R,Maftahi M,Mallet L,Maurer KC,Messenguy F,Mewes HW,M?sti D,Nasr F,Nicaud JM,Niedenthal RK,Pandolfo D,Pi?rard A,Piravandi E,Planta RJ,Pohl TM,Purnelle B,Rebischung C,Remacha M,Revuelta JL,Rinke M,Saiz JE,Sartorello F,Scherens B,Sen-Gupta M,Soler-Mira A,Urbanus JH,Valle G,Van Dyck L,Verhasselt P,Vierendeels F,Vissers S,Voet M,Volckaert G,Wach A,Wambutt R,Wedler H,Zollner A,Hani J


    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


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