doa10 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

  • Gene

  • Clones

  • gRNAs

The following doa10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the doa10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc100664 NM_001022659.2
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Schizosaccharomyces pombe putative ubiquitin ligase Doa10 (doa10), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSc100664
    Clone ID Related Accession (Same CDS sequence) NM_001022659.2
    Accession Version NM_001022659.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product putative ubiquitin ligase Doa10
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001022659.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAACGCAG ATGATGAAAT TTGCCGGGTG TGCCGGTGTG AGGGAGCACC AGATTCACCT 
    CTCTTCCATC CATGTAAATG TACTGGAAGT ATCAGATATG TTCATCAGGA ATGTCTAGTA
    GAGTGGTTAG GTCACAGTAA AAAAACACAT TGTGAACTTT GCAAAGCTAA GTTTGAGTTT
    ACAAAAGTAT ACTCAGAATC TATGCCACGT ACAATCCCTT TTACCATCTT GTGTAGAAAA
    CTTGCTAGTA CTTTAAAACA ACGAGTTATT TTTTTTACTC GTGTTTTACT TACGTTCTTC
    TGCTGGACAG TCCTTCTACC TCTTATATTT AAGCATGTAT GGAATTTGAA CTTTAAAATA
    GGTGACACGT ATACGATTCA TGCAAGAAAT AAAACCTTCA CGGCTCCCCA AAAGCCAGGA
    TATTTTGAAT CAATTTCCCA AATTACGTCC AGTCCTCGGT TGAATACTTT AATTGCCAAC
    ACTGCAGAAG GTCAGGTTCT AACTTTTGTG GTTACATTTA TCTTGATTAC GGCTTTCCTC
    GTTCGTGAAT GGGTTCTTCA AAATGCCGTC CAGGTCGCGG ATGAACTTCA GGGGCAACAG
    TTTGAGAATG TCAACCAGAA TAACCAAGCT CAAGCTGCAG CTGCAGCTGC TCAAAATCTT
    CGTGAAGTTC GTGAAGCTCG TCAACGACTA GCAATGGTGA TGGAGCATTT ACGCGAGCGA
    CAAGAGCAAA GAAATTTAGA ATTGCAAAGA AACGGATCTT TTGAAGAAAT CGAGAGAGCT
    CGTCAGCGAT TTGCTCTTCT GGGGGACAAT ATTCGAGAGC CGCAAGAGGA GGAGAATGAT
    GTTGATGTCG ATGAAATTTT TAATAGGCAA CAACTGAATC AGCCAGCCCT AGACCTTAAT
    GATGCAAATT CTAGTAATTC CGTACCTGTA GAATTTAATT CACTCCATTC TCAAAACGTC
    GATTATCGTG ATGAGGTAGA TTCTTTGCGA CCCCAGTTTA ATGTTGATGA GCAATCTTCA
    ATCTCTCATA GTTCAAATGC TTCGGAGAAT ATTGTAGATG GAGCCGTTAC CCAAGCCAAT
    GGAATTGAAT CTGATTTTAC TAGAGTGGAT CATGAGCCTA TTATTGTCAA TAATGATGAT
    GAAAACGGTA ACAATGAATC AGAGAATGAA GAGGTTATTG AAGAAGATAA TTTAAATAGG
    AATGTTATAG CTGAGGCGCA AAACCAAGTC GTCGCTGACG AAGAAAGAAA TGCCGTTGCT
    CGCGCCGCCC AAATAGCTGA AGCTGATGAT GCTGACGATT TTGACGGTAT ACTAGAATTT
    CTAGGTCTTC GAGGACCCAT TACTGGATTC TTGCAAAATT GCCTCGTTAT TGCTTTCGTC
    GTTTCCGTAT TCTTGACAAC GGCTGTGGGT ATTCCATACA TGTCTGGACG CCTTATGGTT
    GAGTGGATTT TGTTTATCAT TCATCGCCCG ACGTTTATAT TACGTTTTAT ACTATCTTTC
    GTTAACATTT TATTTGACTG GACTGTTGGT GGGGCTTTTA ATATTGTGAA AATATTAACT
    AAACTTCCTC TGTTATCAAC TGTTTTCGTA AAACTTAAGC TTCAAGGAAT TTTTTCTTCT
    TCTTTCCAAC AGGTTTCAAA TAATATGTAT AGTTGGATAT ATGATCATGT ATTTTCTTCT
    TCCGATCATG CCTATGAATC GCTGATATAT TATATGAAAA CTGGACATAA GCAAGTCGTT
    CAATCATTTT CTATTTTTCC GGTTTTCAGG GTTTGCCAAA TGTTTGCTGT CATACTAAAA
    GATTTTGTTG AAAATTACTC TAATAGACCA GTAGACCGAG TTTTTACTAC GCTTATCGGA
    TACTGCATGT TTACGTTTTT AGGGATTTCA TATCTTAATC GCAAACAATT TCTATTTAAT
    GACCCTCAAA TCAGGAATGT GGAATTAGCT TTTAGAGAAG TTTTGAGACA ATGCGGATCT
    ATAGCAAAAT TCGGAATAAT TTTTTCTATC GAATTGGTTG TTTTTCCTAT TTTCTGCGGG
    ATATTGCTAA GCATGTGCTT AATTGGCACT TTCAAAAAGC TTGCTGCCGA GAATCTTCTC
    AATGTAATGA CAGTTTATCC AGCACAAAGC ATTTTTTTGG CATGGTTCAT TGGGATAACA
    TTTATGTTTG AGTTTGCGGT TTTCATTTCC ATGGTACGTA AAATTGTTCG TCCTGGAGTC
    CTGTATTTTT TGCGTGATCC AAATGATCCT CAATTTCATC CAATTCGTGA AATTTTAGAG
    AAACCTATGC TCTTTCAATT GAAGAAAATT GGATTCAGTG CAATACTCTA TTTCGCTTTT
    ATTATTGGCT GTGTTGGTAG CGTTATCCAT CTTTTAAAGT CAACTGGAAT AATTTTTCCT
    ATTGAATTCA CTACTAAGCC TGCTGTGTTT GAAGCACCAA TTGACTTGTT AGCGTTAGAA
    ATTCTCATAT TTTTAAGTAT TAAATTATTT AAACCTCTCG AACTCACTAG AAGTTTTTGG
    CGAACTTTGG TGTCAACATT CTGCAGATGT TTACGTCTAT CATCATATGT TATGGGTCAA
    AGGTATAGTG ATGAAGAGGG TTATTATCCC AAGCAATATT TTTCTTTTCT TAGAAGGATT
    ATAAGCAAAC CTTCGGATAC CGAAAACCAA GATGATGGTG ATAAGCAGAA AGCAAAGAAA
    GACTTTGTCC AAGACGGTTT CTTTTTGTGG TGTCCTTCTA AGGATGTTGT GCCAGTTCGC
    CAAGGAGCCA TGCTTATACC TGTGACAGAA AATGGTTATG AAATTTTTGG TGAAAAAAAG
    AAGGTCGAAG AAAATGCGGA CTATACCATT ACTTATGCAC CTTCTAATTT TTATAAGCGT
    TTGATCGCTT TGTTGTTATT TTGTTGGATA TGCTCAACAC TTGTGACAGT ATTGTTAGTG
    TTTGTTCCAC TTTCATTAGG AAGAGCTATT TATGCTTGGT GCTTTCCTAA TGTGGTAAAG
    CACGATTTTT ATGCTTACGC CATTGGATTT TACTCTATTT CTTTTCCAAT GTATGCAATT
    CACGCGTCTG TTAAGTTTCT GAAACTTGAC TATTTACGAA GCCTTATGAA TAAACTAAAT
    TTAAAAATTG TTATGAGGAG CTTAGTAATG GCGTTAAAAT ATCTTTTATT AGCATTCCTT
    GGAATATTTA TCTTACCACT CTTACTTGGA GCTATATGGG AATTATACGT CGCTATACCT
    TTCCGAACGA TATTTAACAG AGGGACTCTT GCTCTCGATG CATTCCAAAA CTGGGTGATT
    GGTCTTTTTA TGCTTCGTAT GATATATTTT ACTGTGACTT CTAATGAAGA GCGTTTCGTT
    TCAAGGTTGT TTCAAGATGC TTTTCGTGAT AGATGGACGA ATCCTCAAAT TCTTCCATTA
    TTAAAAAATG TTCTCATCCC TTTTACATCA GCCTTAATAG CCGCCGTTGT GTTGCCAAGT
    GTTTTTACTT ACGTAACCTA TCCTTTTCTT TCATCCATTT TCCCTTCTGC ATCTAAAACC
    CTTATGTATC GGTTGATGCA TCCTATATTT TTAGCATTAC TTGGGCTAGC ACTCTTGGGG
    CGCCGTTTTG TAGAAACTAG TAGTAAATGG TCGCAAGGAA TACGTGATGA TTTATACTTA
    GTTGGTACGA GACTGCACAA CTTTGGCGAA TCTGCTCCTC CAGCCATTTC TGAGTCTGCC
    GAAAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_596733.1
    CDS111..3839
    Translation

    Target ORF information:

    RefSeq Version NM_001022659.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe putative ubiquitin ligase Doa10 (doa10), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001022659.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAACGCAG ATGATGAAAT TTGCCGGGTG TGCCGGTGTG AGGGAGCACC AGATTCACCT 
    CTCTTCCATC CATGTAAATG TACTGGAAGT ATCAGATATG TTCATCAGGA ATGTCTAGTA
    GAGTGGTTAG GTCACAGTAA AAAAACACAT TGTGAACTTT GCAAAGCTAA GTTTGAGTTT
    ACAAAAGTAT ACTCAGAATC TATGCCACGT ACAATCCCTT TTACCATCTT GTGTAGAAAA
    CTTGCTAGTA CTTTAAAACA ACGAGTTATT TTTTTTACTC GTGTTTTACT TACGTTCTTC
    TGCTGGACAG TCCTTCTACC TCTTATATTT AAGCATGTAT GGAATTTGAA CTTTAAAATA
    GGTGACACGT ATACGATTCA TGCAAGAAAT AAAACCTTCA CGGCTCCCCA AAAGCCAGGA
    TATTTTGAAT CAATTTCCCA AATTACGTCC AGTCCTCGGT TGAATACTTT AATTGCCAAC
    ACTGCAGAAG GTCAGGTTCT AACTTTTGTG GTTACATTTA TCTTGATTAC GGCTTTCCTC
    GTTCGTGAAT GGGTTCTTCA AAATGCCGTC CAGGTCGCGG ATGAACTTCA GGGGCAACAG
    TTTGAGAATG TCAACCAGAA TAACCAAGCT CAAGCTGCAG CTGCAGCTGC TCAAAATCTT
    CGTGAAGTTC GTGAAGCTCG TCAACGACTA GCAATGGTGA TGGAGCATTT ACGCGAGCGA
    CAAGAGCAAA GAAATTTAGA ATTGCAAAGA AACGGATCTT TTGAAGAAAT CGAGAGAGCT
    CGTCAGCGAT TTGCTCTTCT GGGGGACAAT ATTCGAGAGC CGCAAGAGGA GGAGAATGAT
    GTTGATGTCG ATGAAATTTT TAATAGGCAA CAACTGAATC AGCCAGCCCT AGACCTTAAT
    GATGCAAATT CTAGTAATTC CGTACCTGTA GAATTTAATT CACTCCATTC TCAAAACGTC
    GATTATCGTG ATGAGGTAGA TTCTTTGCGA CCCCAGTTTA ATGTTGATGA GCAATCTTCA
    ATCTCTCATA GTTCAAATGC TTCGGAGAAT ATTGTAGATG GAGCCGTTAC CCAAGCCAAT
    GGAATTGAAT CTGATTTTAC TAGAGTGGAT CATGAGCCTA TTATTGTCAA TAATGATGAT
    GAAAACGGTA ACAATGAATC AGAGAATGAA GAGGTTATTG AAGAAGATAA TTTAAATAGG
    AATGTTATAG CTGAGGCGCA AAACCAAGTC GTCGCTGACG AAGAAAGAAA TGCCGTTGCT
    CGCGCCGCCC AAATAGCTGA AGCTGATGAT GCTGACGATT TTGACGGTAT ACTAGAATTT
    CTAGGTCTTC GAGGACCCAT TACTGGATTC TTGCAAAATT GCCTCGTTAT TGCTTTCGTC
    GTTTCCGTAT TCTTGACAAC GGCTGTGGGT ATTCCATACA TGTCTGGACG CCTTATGGTT
    GAGTGGATTT TGTTTATCAT TCATCGCCCG ACGTTTATAT TACGTTTTAT ACTATCTTTC
    GTTAACATTT TATTTGACTG GACTGTTGGT GGGGCTTTTA ATATTGTGAA AATATTAACT
    AAACTTCCTC TGTTATCAAC TGTTTTCGTA AAACTTAAGC TTCAAGGAAT TTTTTCTTCT
    TCTTTCCAAC AGGTTTCAAA TAATATGTAT AGTTGGATAT ATGATCATGT ATTTTCTTCT
    TCCGATCATG CCTATGAATC GCTGATATAT TATATGAAAA CTGGACATAA GCAAGTCGTT
    CAATCATTTT CTATTTTTCC GGTTTTCAGG GTTTGCCAAA TGTTTGCTGT CATACTAAAA
    GATTTTGTTG AAAATTACTC TAATAGACCA GTAGACCGAG TTTTTACTAC GCTTATCGGA
    TACTGCATGT TTACGTTTTT AGGGATTTCA TATCTTAATC GCAAACAATT TCTATTTAAT
    GACCCTCAAA TCAGGAATGT GGAATTAGCT TTTAGAGAAG TTTTGAGACA ATGCGGATCT
    ATAGCAAAAT TCGGAATAAT TTTTTCTATC GAATTGGTTG TTTTTCCTAT TTTCTGCGGG
    ATATTGCTAA GCATGTGCTT AATTGGCACT TTCAAAAAGC TTGCTGCCGA GAATCTTCTC
    AATGTAATGA CAGTTTATCC AGCACAAAGC ATTTTTTTGG CATGGTTCAT TGGGATAACA
    TTTATGTTTG AGTTTGCGGT TTTCATTTCC ATGGTACGTA AAATTGTTCG TCCTGGAGTC
    CTGTATTTTT TGCGTGATCC AAATGATCCT CAATTTCATC CAATTCGTGA AATTTTAGAG
    AAACCTATGC TCTTTCAATT GAAGAAAATT GGATTCAGTG CAATACTCTA TTTCGCTTTT
    ATTATTGGCT GTGTTGGTAG CGTTATCCAT CTTTTAAAGT CAACTGGAAT AATTTTTCCT
    ATTGAATTCA CTACTAAGCC TGCTGTGTTT GAAGCACCAA TTGACTTGTT AGCGTTAGAA
    ATTCTCATAT TTTTAAGTAT TAAATTATTT AAACCTCTCG AACTCACTAG AAGTTTTTGG
    CGAACTTTGG TGTCAACATT CTGCAGATGT TTACGTCTAT CATCATATGT TATGGGTCAA
    AGGTATAGTG ATGAAGAGGG TTATTATCCC AAGCAATATT TTTCTTTTCT TAGAAGGATT
    ATAAGCAAAC CTTCGGATAC CGAAAACCAA GATGATGGTG ATAAGCAGAA AGCAAAGAAA
    GACTTTGTCC AAGACGGTTT CTTTTTGTGG TGTCCTTCTA AGGATGTTGT GCCAGTTCGC
    CAAGGAGCCA TGCTTATACC TGTGACAGAA AATGGTTATG AAATTTTTGG TGAAAAAAAG
    AAGGTCGAAG AAAATGCGGA CTATACCATT ACTTATGCAC CTTCTAATTT TTATAAGCGT
    TTGATCGCTT TGTTGTTATT TTGTTGGATA TGCTCAACAC TTGTGACAGT ATTGTTAGTG
    TTTGTTCCAC TTTCATTAGG AAGAGCTATT TATGCTTGGT GCTTTCCTAA TGTGGTAAAG
    CACGATTTTT ATGCTTACGC CATTGGATTT TACTCTATTT CTTTTCCAAT GTATGCAATT
    CACGCGTCTG TTAAGTTTCT GAAACTTGAC TATTTACGAA GCCTTATGAA TAAACTAAAT
    TTAAAAATTG TTATGAGGAG CTTAGTAATG GCGTTAAAAT ATCTTTTATT AGCATTCCTT
    GGAATATTTA TCTTACCACT CTTACTTGGA GCTATATGGG AATTATACGT CGCTATACCT
    TTCCGAACGA TATTTAACAG AGGGACTCTT GCTCTCGATG CATTCCAAAA CTGGGTGATT
    GGTCTTTTTA TGCTTCGTAT GATATATTTT ACTGTGACTT CTAATGAAGA GCGTTTCGTT
    TCAAGGTTGT TTCAAGATGC TTTTCGTGAT AGATGGACGA ATCCTCAAAT TCTTCCATTA
    TTAAAAAATG TTCTCATCCC TTTTACATCA GCCTTAATAG CCGCCGTTGT GTTGCCAAGT
    GTTTTTACTT ACGTAACCTA TCCTTTTCTT TCATCCATTT TCCCTTCTGC ATCTAAAACC
    CTTATGTATC GGTTGATGCA TCCTATATTT TTAGCATTAC TTGGGCTAGC ACTCTTGGGG
    CGCCGTTTTG TAGAAACTAG TAGTAAATGG TCGCAAGGAA TACGTGATGA TTTATACTTA
    GTTGGTACGA GACTGCACAA CTTTGGCGAA TCTGCTCCTC CAGCCATTTC TGAGTCTGCC
    GAAAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L


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