SPAC227.12 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

  • Gene

  • Clones

  • gRNAs

The following SPAC227.12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPAC227.12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc103238 NM_001018366.2
Latest version!
Schizosaccharomyces pombe putative Prp4 family U4/U5/U6 small nuclear ribonucleoprotein complex subunit (SPAC227.12), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSc103238
    Clone ID Related Accession (Same CDS sequence) NM_001018366.2
    Accession Version NM_001018366.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1389bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product putative Prp4 family U4/U5/U6 small nuclear ribonucleoprotein complex subunit
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001018366.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGAATGAAA ACGAGGGCAT TTCACTTGGT GAATTAGAAA CAAGACCGTT TTCAGAAGGG 
    ATAACGCGGT TTACAAAAGA ACAAGCAGCT TATTACGCGG AGCAAAAGGA AAAGGATCGC
    ATTAAGGCTC TTAAAATTCC TTATGAAGAT TCCAAAGTTC GTGAATATCT GCGTCGTTAT
    GGTGAACCCA TAACTTATTT TGGTGAAGAT GCCTTAGCCC GACGCCAAAG GCTTCAGCAA
    TTAATGATCG AAAAGTCCCT AGAAGGAGAC AATCCATTAG ATGTAGACCA AGGGGCTTCT
    GAAAATATTG AGAAGGAGAC GTATGTTCAA GGAAGTCACG AACTATTAGT TGCTCGTAAA
    AAAATAGCTC TTTACTCCTT GGAAAAGGCA AAGCTCAGAT TAAAAAAAGA ACGGGAAATT
    TCCGAGATTC CAGTACCTGA AATTGTACTT TCCGGAAAGT CTTCAATCGA ACATTTACAA
    AAGGCTGAAT TGATGGGCTC TCAGATAGGT GGTGAGCGAC CAATAGCCAT TGTTAGATTC
    AGTAATAATG GAAATCATTT TGCTAGCGGA AGCTGGGGTG GCCAGGTCAA GGTGTGGAAT
    TCTGATAACC TCTCAGAGGT TCAATTGTTT CGCGGCCATA CCGACCGAGT TTCTGGTTTG
    GATTGGTATC CTCTATGTCA GGCATGGGAT GCAGATTCTG AGCAGCTGAC TTTAGCGACT
    GGAGCCGCAG ACAATACTGT TTGTTTGTGG AAAGCAAGTC AATCTACTCC ACTGTTACGT
    TTGGAAGGAC ATTTGGCTCG TGTTGGTCGA GTTGCTTTTC ATCCAAGTGG TGATTACTTA
    GTTTCTGCCT CATTCGACAC TACATGGCGG TTATGGGATG TACATACAGG CGTTGAGTTG
    CTTATGCAAG AAGGTCATTC GGAAGGGATT TTTTCAATCG CCTGTCAGCC AGATGGGTCG
    TTAGTTAGCT CAGGTGGTAA TGATGCTATC GGTCGGATTT GGGATCTTCG ATCAGGCAAA
    TCAATAATGG TATTAGATGA ACATATTCGT CAAATTGTCG CGATGGCTTG GAGTCCAAAC
    GGCTACCAAC TTGCAACTTC TTCAGCCGAT GATACTGTGA AAATATGGGA CTTGCGGAAA
    GTGTCATTGG CACATACTAT TCCCGCACAT TCTTCTCTTG TTTCAGACGT TCGTTATATT
    GAATCAGGTG TTAACAGATT CATCGCTACG AGTGGTTATG ATGGCTGCGT AAAGCTTTGG
    AACCCGTTGA ACTGCTCATT GATTAAAAGT ATGGTGGGAC ACGAAGAAAA GGTTATGAGT
    GTTGACGGTT ACGGAGACAG ATTTATCTCA TCAGGATACG ATCGTACAAT CAAGTTATGG
    TACCCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_592966.1
    CDS137..1525
    Translation

    Target ORF information:

    RefSeq Version NM_001018366.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe putative Prp4 family U4/U5/U6 small nuclear ribonucleoprotein complex subunit (SPAC227.12), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001018366.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGAATGAAA ACGAGGGCAT TTCACTTGGT GAATTAGAAA CAAGACCGTT TTCAGAAGGG 
    ATAACGCGGT TTACAAAAGA ACAAGCAGCT TATTACGCGG AGCAAAAGGA AAAGGATCGC
    ATTAAGGCTC TTAAAATTCC TTATGAAGAT TCCAAAGTTC GTGAATATCT GCGTCGTTAT
    GGTGAACCCA TAACTTATTT TGGTGAAGAT GCCTTAGCCC GACGCCAAAG GCTTCAGCAA
    TTAATGATCG AAAAGTCCCT AGAAGGAGAC AATCCATTAG ATGTAGACCA AGGGGCTTCT
    GAAAATATTG AGAAGGAGAC GTATGTTCAA GGAAGTCACG AACTATTAGT TGCTCGTAAA
    AAAATAGCTC TTTACTCCTT GGAAAAGGCA AAGCTCAGAT TAAAAAAAGA ACGGGAAATT
    TCCGAGATTC CAGTACCTGA AATTGTACTT TCCGGAAAGT CTTCAATCGA ACATTTACAA
    AAGGCTGAAT TGATGGGCTC TCAGATAGGT GGTGAGCGAC CAATAGCCAT TGTTAGATTC
    AGTAATAATG GAAATCATTT TGCTAGCGGA AGCTGGGGTG GCCAGGTCAA GGTGTGGAAT
    TCTGATAACC TCTCAGAGGT TCAATTGTTT CGCGGCCATA CCGACCGAGT TTCTGGTTTG
    GATTGGTATC CTCTATGTCA GGCATGGGAT GCAGATTCTG AGCAGCTGAC TTTAGCGACT
    GGAGCCGCAG ACAATACTGT TTGTTTGTGG AAAGCAAGTC AATCTACTCC ACTGTTACGT
    TTGGAAGGAC ATTTGGCTCG TGTTGGTCGA GTTGCTTTTC ATCCAAGTGG TGATTACTTA
    GTTTCTGCCT CATTCGACAC TACATGGCGG TTATGGGATG TACATACAGG CGTTGAGTTG
    CTTATGCAAG AAGGTCATTC GGAAGGGATT TTTTCAATCG CCTGTCAGCC AGATGGGTCG
    TTAGTTAGCT CAGGTGGTAA TGATGCTATC GGTCGGATTT GGGATCTTCG ATCAGGCAAA
    TCAATAATGG TATTAGATGA ACATATTCGT CAAATTGTCG CGATGGCTTG GAGTCCAAAC
    GGCTACCAAC TTGCAACTTC TTCAGCCGAT GATACTGTGA AAATATGGGA CTTGCGGAAA
    GTGTCATTGG CACATACTAT TCCCGCACAT TCTTCTCTTG TTTCAGACGT TCGTTATATT
    GAATCAGGTG TTAACAGATT CATCGCTACG AGTGGTTATG ATGGCTGCGT AAAGCTTTGG
    AACCCGTTGA ACTGCTCATT GATTAAAAGT ATGGTGGGAC ACGAAGAAAA GGTTATGAGT
    GTTGACGGTT ACGGAGACAG ATTTATCTCA TCAGGATACG ATCGTACAAT CAAGTTATGG
    TACCCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L


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