HFM1 cDNA ORF clone, Sus scrofa(pig)

  • Gene

  • Clones

  • gRNAs

The following HFM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HFM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSe248242 XM_021090252.1
Latest version!
Sus scrofa HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OSe248242 XM_021090255.1
Latest version!
Sus scrofa HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OSe248242 XM_021090251.1
Latest version!
Sus scrofa HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OSe248242 XM_021090254.1
Latest version!
Sus scrofa HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OSe248242 XM_021090253.1
Latest version!
Sus scrofa HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OSe248243 XM_013997383.2
Latest version!
Sus scrofa HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OSe248244 XM_013997386.2
Latest version!
Sus scrofa HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OSe248245 XM_021090256.1
Latest version!
Sus scrofa HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OSe248245 XM_021090257.1
Latest version!
Sus scrofa HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OSe248246 XM_013997387.2
Latest version!
Sus scrofa HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OSe248247 XM_013997388.2
Latest version!
Sus scrofa HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OSe248248 XM_021090259.1
Latest version!
Sus scrofa HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OSe248248 XM_021090260.1
Latest version!
Sus scrofa HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OSe248248 XM_021090258.1
Latest version!
Sus scrofa HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OSe35333 XM_013997383.1
Latest version!
Sus scrofa HFM1, ATP-dependent DNA helicase homolog (HFM1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OSe35333 XM_013997384.1
Latest version!
Sus scrofa HFM1, ATP-dependent DNA helicase homolog (HFM1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OSe35333 XM_013997385.1
Latest version!
Sus scrofa HFM1, ATP-dependent DNA helicase homolog (HFM1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OSe35334 XM_013997387.1
Latest version!
Sus scrofa HFM1, ATP-dependent DNA helicase homolog (HFM1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OSe35335 XM_013997388.1
Latest version!
Sus scrofa HFM1, ATP-dependent DNA helicase homolog (HFM1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OSe35336 XM_013997389.1
Latest version!
Sus scrofa HFM1, ATP-dependent DNA helicase homolog (HFM1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OSe35336 XM_013997390.1
Latest version!
Sus scrofa HFM1, ATP-dependent DNA helicase homolog (HFM1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OSe09310 XM_013997386.1
Latest version!
Sus scrofa HFM1, ATP-dependent DNA helicase homolog (HFM1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSe248242
    Clone ID Related Accession (Same CDS sequence) XM_021090254.1 , XM_021090252.1 , XM_021090255.1 , XM_021090251.1 , XM_021090253.1
    Accession Version XM_021090254.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4458bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product probable ATP-dependent DNA helicase HFM1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010446.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCTGAGAT CACATGACTC CTTGTGTTCT TTGGATAATT TATTTTTTGA AAAAACAGAT 
    GAAGCTGAAA AATACCCAGA CAATGAAAAA TCATTAGATT GGTTTCTCCC ACCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAAGCA TTAGAGGAAG ACATGCAAAG TCATAAACTG
    TTAGGTCAGA AAAAGAGGTC AAAAATGTTA ACATCCAATT TAAAGATAAC TAATGAAGAC
    ACAAGTTATA CTTCACCAAC ACAAAAATTC CAGTGTGCTT TCCCTTCTAA TAAATACGAA
    GAAGGCGATC TAAATTTAGT AGGGGCAGGT AGCAATGACT TACATGTTGC TGGCAAGCTG
    ACTTATGGTT CTCCTCAGAA GTACAAAAGT CACATTGGCC CTGAGATAGC ACTTGAGAAA
    AATGTTCCAG ATGATGCGAA AGTAGTTAAT TTTGCATACG ATAAAGGAGA GAGCACATCG
    GCCTTCCGAA AAAGATTATT TAAAATATCT GATAGTATAC ATGGGAATGC TTGTTCTAAT
    GACATTGCAA ACTTGGACTC TGATATTGGC TCAGCGAAGA TTGCCCAAAT GGAAATGAGC
    GAAGGGAAAT CGTGGAAATG GAACAATAGT AATCAGAAAC CTCAATATTC AGAAATTAAC
    ATGTTTACAG CCAGTGATAC TTTTTCTGCT TCTAAAATCA GAGAAGGCAT ATTCAAAGCA
    CCATCTTTTT CAGTTGCATC CCAGCCTCAT GAAGTTCAAG GGATAATAGA AAATGGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATCCCG GCAAAATTTA GAAGTGTTTT CAAAGAATTC
    CCATATTTCA ACTATATCCA GTCTAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    GTTGTGGTTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAA TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGCAGTCA GCGTTTTGAT GACTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC GGGAGATACA GTCATGGATG ACCTGTTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCAACTGGT TCGACTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTT GTCAGCAGGA TGAAAACTGT GCAGTTTTTA
    TCTCATGCTG TGGAAAATCC CAGAAATATT ATTCCAATGA GATTTGTAGC TGTATCTGCA
    ACAATTCCAA ATGCTGAGGA TATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCCGCTATC
    TGTCTGAAAA TGGATGAGAA ACATAGACCA GTGAAGCTTC GGAAAGTGGT CCTTGGTTTT
    CCCTGCAGTA ATAACCAAAC TGAATTTAAG TTTGATTTAA CCCTCAACTA CAAAATTGCC
    AGTGTTGTAC AAACGTACTC CGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTACAAC AGGCTGCTTC CATTCTTGTG AAAGATGCCA AGTTTATTAT GACGGTGGAA
    CAGAAACAGA GGTTACATAA ATGTGCATAT TCCATAAGAG ATTCAAAATT GAGAGATCTC
    TTAATACACG GTGTTGCTTA TCATCATGCT GGCATGGAGC TGTCAGATAG AAAAGTAGTC
    GAAGGAGCTT TTACTGTTGG AGATCTACCA GTTCTTTTTA CTACTAGTAC CTTAGCTATG
    GGAGTAAATT TGCCTGCTCA TCTAGTAGTT ATCAAATCTA CAATGCATTA TGCTGGAGGG
    ATGTTTGAAG AGTACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCAAC TGCAGTTATT ATGACTCGGT TAAGTACAAA AGAGAAGTAT
    ATTCAGATGC TGGCTTGTAG TGACACTGTA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT GTTGCATACC ATCACAGATG TCAACATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTGTA TATCAGAGCC TTGAAGAATC CGTCTCACTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATTTG
    AATGATTTAT CATCTCTGGA CTTGATAAAG ATGGATGAAG ATATTAATTT CAAGCCAACT
    GAGGCAGGAA GATTGATGGC TTGGTACTAT GTTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATCAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG GATAAATGAA AAGAAAACAC TGAATACTTT GAACAAAGAT
    CCAAATCGGA TAACTATCAG ATTTCCAATG GAAGGAAAAA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCA TACAAGACTT TGCTTTGACA
    CAAGATACCT CAAAGATTTT CAGGAATGGC TCACGAATTA CGAGATGGTT ATCAGATTTT
    GTGGCTGTTC AAGAAAAGTT TGCTGTGCTT TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCTGCAT GTATCCAAAC AACTGGAAAA AATCGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AGCTTGAACT GATTTTAAAT AGACATCCCC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTGTCTGCC AAAATACGAA CTTGAAGTGG AACAGATTGC AAGATACAGT
    GATACCACGG CAGAAGTTTT AGTGACTGTT ATATTAAGAA ACTTCGAACC ACTGCAAACT
    AAAAGAACAG CACCAGATTC TCACTATGTT ACCTTAATCA TAGGCGATGC AGATAATCAG
    CTGATTTTTA AGCATAAAAT TATGGATTCT GTTTTGCTGA AATCTGGAAA TTGGGCTAAA
    AAGATCGATG TGAAGAGAGC TCTGAAATCT GAAGATCTTA GCATAAATCT CATTAGTTCT
    GAGTATGTTG GACTTGATAT TCAGCAGAAA TTTACAGTCT TTTACTTAGG ACCCAAGAGA
    TTTGGAAATC AAATAATTAT GCAAAAAAAA TCAGAAACAG AGATCTTCCA TTCTAAACGT
    TCAGATGGAT CTCCTGTAGC AGGTCCCATT AAAGGAATGA CTGCCTGCAG AAAGCCTGGG
    CACCGAGAGT GTAACCATCA CTGTAAAAAG AAACACACGT GTGGACATGA CTGCTGTAAA
    ACTGGAGTTG CCCAGAAGCC AGAAATGAAA GAGTCAACAA TTTCTTCTTA TTTATCTGAT
    TTAAGAAACA GGAATGCAGT TTCATCTCTT CCTCCAGTTA AACGTCTAAA GATGCAGATG
    AATAAATCTC AAAGTGTGGA CCTTAAAGAG TTTGGTTTTA CTCCACAACC TTCTCTTCCC
    AGTCTGTCAA GGTCAGAATG TTTAAACATA CCTAAATTGC CTGTAATGGA GCAGAGGGGT
    CAGCACGAGC TCTATGCAGA AGGTCAACAA CAAGAACCAC CGGGATATCA GGACAAAGGT
    TTTTCCAGAC AGACCTCTTC TACAACCTCA AATGAGAAAA CCCCTCAAAC AACACTGTTC
    AACAGAAATA TTAAGGCAAC TGGAAACCAG AATTTGACAG CTGTGGTTAA AAGTAATTCT
    TCCTATTCAG AAGTTTTGAA TGTGAACTTT GAATTGGGAA ATGAAGTTTG GGATGATTTT
    GATGATGAAA ACTTAATAGA AATTGCTAGC TTTTCAGCTG ATACTGAAAA GACAAAAGCA
    GGATTTGGAA ACACTTTGAG TTCAAGTGCC AGGGAAAATA AGCTACCCTT CCAAGAATCA
    AAAAGCAAAT TCCAAAGAAA AATGTCAAAC AGTTTTGTTT CATCACATGA GAAGCCAGAT
    ATTTATTTAC CAAATTCTGC TCTGTCCAGG ATCGGTGCAT CCTCCATGAC AAAAATACCT
    CAACCAGCTG GAAATGTCCC TTTCCAAGAA GTAAAACAAC AGAATCTGTC ACCAGAGATT
    GAAAAGCTAT GCTTTGCTCA CTCTGAAAAA ATATCAAGCT TTTCAAATTC TGTTACGCAA
    GTGGATTGCT TTATTAGAAA CAGTGAATAT AAAAAGCAAC CTGACTTCAG TAAGTTATAT
    TTCTTGCCTC GTCATAAGAA ACAATATGGA TTATACGTTA AATATACAAG CTGCCAGACT
    TCATCCCAGA GCTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020945913.1
    CDS1113..5570
    Translation

    Target ORF information:

    RefSeq Version XM_021090254.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021090254.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCTGAGAT CACATGACTC CTTGTGTTCT TTGGATAATT TATTTTTTGA AAAAACAGAT 
    GAAGCTGAAA AATACCCAGA CAATGAAAAA TCATTAGATT GGTTTCTCCC ACCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAAGCA TTAGAGGAAG ACATGCAAAG TCATAAACTG
    TTAGGTCAGA AAAAGAGGTC AAAAATGTTA ACATCCAATT TAAAGATAAC TAATGAAGAC
    ACAAGTTATA CTTCACCAAC ACAAAAATTC CAGTGTGCTT TCCCTTCTAA TAAATACGAA
    GAAGGCGATC TAAATTTAGT AGGGGCAGGT AGCAATGACT TACATGTTGC TGGCAAGCTG
    ACTTATGGTT CTCCTCAGAA GTACAAAAGT CACATTGGCC CTGAGATAGC ACTTGAGAAA
    AATGTTCCAG ATGATGCGAA AGTAGTTAAT TTTGCATACG ATAAAGGAGA GAGCACATCG
    GCCTTCCGAA AAAGATTATT TAAAATATCT GATAGTATAC ATGGGAATGC TTGTTCTAAT
    GACATTGCAA ACTTGGACTC TGATATTGGC TCAGCGAAGA TTGCCCAAAT GGAAATGAGC
    GAAGGGAAAT CGTGGAAATG GAACAATAGT AATCAGAAAC CTCAATATTC AGAAATTAAC
    ATGTTTACAG CCAGTGATAC TTTTTCTGCT TCTAAAATCA GAGAAGGCAT ATTCAAAGCA
    CCATCTTTTT CAGTTGCATC CCAGCCTCAT GAAGTTCAAG GGATAATAGA AAATGGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATCCCG GCAAAATTTA GAAGTGTTTT CAAAGAATTC
    CCATATTTCA ACTATATCCA GTCTAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    GTTGTGGTTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAA TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGCAGTCA GCGTTTTGAT GACTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC GGGAGATACA GTCATGGATG ACCTGTTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCAACTGGT TCGACTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTT GTCAGCAGGA TGAAAACTGT GCAGTTTTTA
    TCTCATGCTG TGGAAAATCC CAGAAATATT ATTCCAATGA GATTTGTAGC TGTATCTGCA
    ACAATTCCAA ATGCTGAGGA TATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCCGCTATC
    TGTCTGAAAA TGGATGAGAA ACATAGACCA GTGAAGCTTC GGAAAGTGGT CCTTGGTTTT
    CCCTGCAGTA ATAACCAAAC TGAATTTAAG TTTGATTTAA CCCTCAACTA CAAAATTGCC
    AGTGTTGTAC AAACGTACTC CGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTACAAC AGGCTGCTTC CATTCTTGTG AAAGATGCCA AGTTTATTAT GACGGTGGAA
    CAGAAACAGA GGTTACATAA ATGTGCATAT TCCATAAGAG ATTCAAAATT GAGAGATCTC
    TTAATACACG GTGTTGCTTA TCATCATGCT GGCATGGAGC TGTCAGATAG AAAAGTAGTC
    GAAGGAGCTT TTACTGTTGG AGATCTACCA GTTCTTTTTA CTACTAGTAC CTTAGCTATG
    GGAGTAAATT TGCCTGCTCA TCTAGTAGTT ATCAAATCTA CAATGCATTA TGCTGGAGGG
    ATGTTTGAAG AGTACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCAAC TGCAGTTATT ATGACTCGGT TAAGTACAAA AGAGAAGTAT
    ATTCAGATGC TGGCTTGTAG TGACACTGTA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT GTTGCATACC ATCACAGATG TCAACATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTGTA TATCAGAGCC TTGAAGAATC CGTCTCACTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATTTG
    AATGATTTAT CATCTCTGGA CTTGATAAAG ATGGATGAAG ATATTAATTT CAAGCCAACT
    GAGGCAGGAA GATTGATGGC TTGGTACTAT GTTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATCAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG GATAAATGAA AAGAAAACAC TGAATACTTT GAACAAAGAT
    CCAAATCGGA TAACTATCAG ATTTCCAATG GAAGGAAAAA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCA TACAAGACTT TGCTTTGACA
    CAAGATACCT CAAAGATTTT CAGGAATGGC TCACGAATTA CGAGATGGTT ATCAGATTTT
    GTGGCTGTTC AAGAAAAGTT TGCTGTGCTT TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCTGCAT GTATCCAAAC AACTGGAAAA AATCGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AGCTTGAACT GATTTTAAAT AGACATCCCC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTGTCTGCC AAAATACGAA CTTGAAGTGG AACAGATTGC AAGATACAGT
    GATACCACGG CAGAAGTTTT AGTGACTGTT ATATTAAGAA ACTTCGAACC ACTGCAAACT
    AAAAGAACAG CACCAGATTC TCACTATGTT ACCTTAATCA TAGGCGATGC AGATAATCAG
    CTGATTTTTA AGCATAAAAT TATGGATTCT GTTTTGCTGA AATCTGGAAA TTGGGCTAAA
    AAGATCGATG TGAAGAGAGC TCTGAAATCT GAAGATCTTA GCATAAATCT CATTAGTTCT
    GAGTATGTTG GACTTGATAT TCAGCAGAAA TTTACAGTCT TTTACTTAGG ACCCAAGAGA
    TTTGGAAATC AAATAATTAT GCAAAAAAAA TCAGAAACAG AGATCTTCCA TTCTAAACGT
    TCAGATGGAT CTCCTGTAGC AGGTCCCATT AAAGGAATGA CTGCCTGCAG AAAGCCTGGG
    CACCGAGAGT GTAACCATCA CTGTAAAAAG AAACACACGT GTGGACATGA CTGCTGTAAA
    ACTGGAGTTG CCCAGAAGCC AGAAATGAAA GAGTCAACAA TTTCTTCTTA TTTATCTGAT
    TTAAGAAACA GGAATGCAGT TTCATCTCTT CCTCCAGTTA AACGTCTAAA GATGCAGATG
    AATAAATCTC AAAGTGTGGA CCTTAAAGAG TTTGGTTTTA CTCCACAACC TTCTCTTCCC
    AGTCTGTCAA GGTCAGAATG TTTAAACATA CCTAAATTGC CTGTAATGGA GCAGAGGGGT
    CAGCACGAGC TCTATGCAGA AGGTCAACAA CAAGAACCAC CGGGATATCA GGACAAAGGT
    TTTTCCAGAC AGACCTCTTC TACAACCTCA AATGAGAAAA CCCCTCAAAC AACACTGTTC
    AACAGAAATA TTAAGGCAAC TGGAAACCAG AATTTGACAG CTGTGGTTAA AAGTAATTCT
    TCCTATTCAG AAGTTTTGAA TGTGAACTTT GAATTGGGAA ATGAAGTTTG GGATGATTTT
    GATGATGAAA ACTTAATAGA AATTGCTAGC TTTTCAGCTG ATACTGAAAA GACAAAAGCA
    GGATTTGGAA ACACTTTGAG TTCAAGTGCC AGGGAAAATA AGCTACCCTT CCAAGAATCA
    AAAAGCAAAT TCCAAAGAAA AATGTCAAAC AGTTTTGTTT CATCACATGA GAAGCCAGAT
    ATTTATTTAC CAAATTCTGC TCTGTCCAGG ATCGGTGCAT CCTCCATGAC AAAAATACCT
    CAACCAGCTG GAAATGTCCC TTTCCAAGAA GTAAAACAAC AGAATCTGTC ACCAGAGATT
    GAAAAGCTAT GCTTTGCTCA CTCTGAAAAA ATATCAAGCT TTTCAAATTC TGTTACGCAA
    GTGGATTGCT TTATTAGAAA CAGTGAATAT AAAAAGCAAC CTGACTTCAG TAAGTTATAT
    TTCTTGCCTC GTCATAAGAA ACAATATGGA TTATACGTTA AATATACAAG CTGCCAGACT
    TCATCCCAGA GCTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe248242
    Clone ID Related Accession (Same CDS sequence) XM_021090254.1 , XM_021090252.1 , XM_021090255.1 , XM_021090251.1 , XM_021090253.1
    Accession Version XM_021090252.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4458bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product probable ATP-dependent DNA helicase HFM1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010446.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCTGAGAT CACATGACTC CTTGTGTTCT TTGGATAATT TATTTTTTGA AAAAACAGAT 
    GAAGCTGAAA AATACCCAGA CAATGAAAAA TCATTAGATT GGTTTCTCCC ACCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAAGCA TTAGAGGAAG ACATGCAAAG TCATAAACTG
    TTAGGTCAGA AAAAGAGGTC AAAAATGTTA ACATCCAATT TAAAGATAAC TAATGAAGAC
    ACAAGTTATA CTTCACCAAC ACAAAAATTC CAGTGTGCTT TCCCTTCTAA TAAATACGAA
    GAAGGCGATC TAAATTTAGT AGGGGCAGGT AGCAATGACT TACATGTTGC TGGCAAGCTG
    ACTTATGGTT CTCCTCAGAA GTACAAAAGT CACATTGGCC CTGAGATAGC ACTTGAGAAA
    AATGTTCCAG ATGATGCGAA AGTAGTTAAT TTTGCATACG ATAAAGGAGA GAGCACATCG
    GCCTTCCGAA AAAGATTATT TAAAATATCT GATAGTATAC ATGGGAATGC TTGTTCTAAT
    GACATTGCAA ACTTGGACTC TGATATTGGC TCAGCGAAGA TTGCCCAAAT GGAAATGAGC
    GAAGGGAAAT CGTGGAAATG GAACAATAGT AATCAGAAAC CTCAATATTC AGAAATTAAC
    ATGTTTACAG CCAGTGATAC TTTTTCTGCT TCTAAAATCA GAGAAGGCAT ATTCAAAGCA
    CCATCTTTTT CAGTTGCATC CCAGCCTCAT GAAGTTCAAG GGATAATAGA AAATGGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATCCCG GCAAAATTTA GAAGTGTTTT CAAAGAATTC
    CCATATTTCA ACTATATCCA GTCTAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    GTTGTGGTTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAA TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGCAGTCA GCGTTTTGAT GACTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC GGGAGATACA GTCATGGATG ACCTGTTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCAACTGGT TCGACTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTT GTCAGCAGGA TGAAAACTGT GCAGTTTTTA
    TCTCATGCTG TGGAAAATCC CAGAAATATT ATTCCAATGA GATTTGTAGC TGTATCTGCA
    ACAATTCCAA ATGCTGAGGA TATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCCGCTATC
    TGTCTGAAAA TGGATGAGAA ACATAGACCA GTGAAGCTTC GGAAAGTGGT CCTTGGTTTT
    CCCTGCAGTA ATAACCAAAC TGAATTTAAG TTTGATTTAA CCCTCAACTA CAAAATTGCC
    AGTGTTGTAC AAACGTACTC CGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTACAAC AGGCTGCTTC CATTCTTGTG AAAGATGCCA AGTTTATTAT GACGGTGGAA
    CAGAAACAGA GGTTACATAA ATGTGCATAT TCCATAAGAG ATTCAAAATT GAGAGATCTC
    TTAATACACG GTGTTGCTTA TCATCATGCT GGCATGGAGC TGTCAGATAG AAAAGTAGTC
    GAAGGAGCTT TTACTGTTGG AGATCTACCA GTTCTTTTTA CTACTAGTAC CTTAGCTATG
    GGAGTAAATT TGCCTGCTCA TCTAGTAGTT ATCAAATCTA CAATGCATTA TGCTGGAGGG
    ATGTTTGAAG AGTACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCAAC TGCAGTTATT ATGACTCGGT TAAGTACAAA AGAGAAGTAT
    ATTCAGATGC TGGCTTGTAG TGACACTGTA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT GTTGCATACC ATCACAGATG TCAACATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTGTA TATCAGAGCC TTGAAGAATC CGTCTCACTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATTTG
    AATGATTTAT CATCTCTGGA CTTGATAAAG ATGGATGAAG ATATTAATTT CAAGCCAACT
    GAGGCAGGAA GATTGATGGC TTGGTACTAT GTTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATCAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG GATAAATGAA AAGAAAACAC TGAATACTTT GAACAAAGAT
    CCAAATCGGA TAACTATCAG ATTTCCAATG GAAGGAAAAA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCA TACAAGACTT TGCTTTGACA
    CAAGATACCT CAAAGATTTT CAGGAATGGC TCACGAATTA CGAGATGGTT ATCAGATTTT
    GTGGCTGTTC AAGAAAAGTT TGCTGTGCTT TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCTGCAT GTATCCAAAC AACTGGAAAA AATCGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AGCTTGAACT GATTTTAAAT AGACATCCCC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTGTCTGCC AAAATACGAA CTTGAAGTGG AACAGATTGC AAGATACAGT
    GATACCACGG CAGAAGTTTT AGTGACTGTT ATATTAAGAA ACTTCGAACC ACTGCAAACT
    AAAAGAACAG CACCAGATTC TCACTATGTT ACCTTAATCA TAGGCGATGC AGATAATCAG
    CTGATTTTTA AGCATAAAAT TATGGATTCT GTTTTGCTGA AATCTGGAAA TTGGGCTAAA
    AAGATCGATG TGAAGAGAGC TCTGAAATCT GAAGATCTTA GCATAAATCT CATTAGTTCT
    GAGTATGTTG GACTTGATAT TCAGCAGAAA TTTACAGTCT TTTACTTAGG ACCCAAGAGA
    TTTGGAAATC AAATAATTAT GCAAAAAAAA TCAGAAACAG AGATCTTCCA TTCTAAACGT
    TCAGATGGAT CTCCTGTAGC AGGTCCCATT AAAGGAATGA CTGCCTGCAG AAAGCCTGGG
    CACCGAGAGT GTAACCATCA CTGTAAAAAG AAACACACGT GTGGACATGA CTGCTGTAAA
    ACTGGAGTTG CCCAGAAGCC AGAAATGAAA GAGTCAACAA TTTCTTCTTA TTTATCTGAT
    TTAAGAAACA GGAATGCAGT TTCATCTCTT CCTCCAGTTA AACGTCTAAA GATGCAGATG
    AATAAATCTC AAAGTGTGGA CCTTAAAGAG TTTGGTTTTA CTCCACAACC TTCTCTTCCC
    AGTCTGTCAA GGTCAGAATG TTTAAACATA CCTAAATTGC CTGTAATGGA GCAGAGGGGT
    CAGCACGAGC TCTATGCAGA AGGTCAACAA CAAGAACCAC CGGGATATCA GGACAAAGGT
    TTTTCCAGAC AGACCTCTTC TACAACCTCA AATGAGAAAA CCCCTCAAAC AACACTGTTC
    AACAGAAATA TTAAGGCAAC TGGAAACCAG AATTTGACAG CTGTGGTTAA AAGTAATTCT
    TCCTATTCAG AAGTTTTGAA TGTGAACTTT GAATTGGGAA ATGAAGTTTG GGATGATTTT
    GATGATGAAA ACTTAATAGA AATTGCTAGC TTTTCAGCTG ATACTGAAAA GACAAAAGCA
    GGATTTGGAA ACACTTTGAG TTCAAGTGCC AGGGAAAATA AGCTACCCTT CCAAGAATCA
    AAAAGCAAAT TCCAAAGAAA AATGTCAAAC AGTTTTGTTT CATCACATGA GAAGCCAGAT
    ATTTATTTAC CAAATTCTGC TCTGTCCAGG ATCGGTGCAT CCTCCATGAC AAAAATACCT
    CAACCAGCTG GAAATGTCCC TTTCCAAGAA GTAAAACAAC AGAATCTGTC ACCAGAGATT
    GAAAAGCTAT GCTTTGCTCA CTCTGAAAAA ATATCAAGCT TTTCAAATTC TGTTACGCAA
    GTGGATTGCT TTATTAGAAA CAGTGAATAT AAAAAGCAAC CTGACTTCAG TAAGTTATAT
    TTCTTGCCTC GTCATAAGAA ACAATATGGA TTATACGTTA AATATACAAG CTGCCAGACT
    TCATCCCAGA GCTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020945911.1
    CDS103..4560
    Translation

    Target ORF information:

    RefSeq Version XM_021090252.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021090252.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCTGAGAT CACATGACTC CTTGTGTTCT TTGGATAATT TATTTTTTGA AAAAACAGAT 
    GAAGCTGAAA AATACCCAGA CAATGAAAAA TCATTAGATT GGTTTCTCCC ACCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAAGCA TTAGAGGAAG ACATGCAAAG TCATAAACTG
    TTAGGTCAGA AAAAGAGGTC AAAAATGTTA ACATCCAATT TAAAGATAAC TAATGAAGAC
    ACAAGTTATA CTTCACCAAC ACAAAAATTC CAGTGTGCTT TCCCTTCTAA TAAATACGAA
    GAAGGCGATC TAAATTTAGT AGGGGCAGGT AGCAATGACT TACATGTTGC TGGCAAGCTG
    ACTTATGGTT CTCCTCAGAA GTACAAAAGT CACATTGGCC CTGAGATAGC ACTTGAGAAA
    AATGTTCCAG ATGATGCGAA AGTAGTTAAT TTTGCATACG ATAAAGGAGA GAGCACATCG
    GCCTTCCGAA AAAGATTATT TAAAATATCT GATAGTATAC ATGGGAATGC TTGTTCTAAT
    GACATTGCAA ACTTGGACTC TGATATTGGC TCAGCGAAGA TTGCCCAAAT GGAAATGAGC
    GAAGGGAAAT CGTGGAAATG GAACAATAGT AATCAGAAAC CTCAATATTC AGAAATTAAC
    ATGTTTACAG CCAGTGATAC TTTTTCTGCT TCTAAAATCA GAGAAGGCAT ATTCAAAGCA
    CCATCTTTTT CAGTTGCATC CCAGCCTCAT GAAGTTCAAG GGATAATAGA AAATGGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATCCCG GCAAAATTTA GAAGTGTTTT CAAAGAATTC
    CCATATTTCA ACTATATCCA GTCTAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    GTTGTGGTTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAA TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGCAGTCA GCGTTTTGAT GACTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC GGGAGATACA GTCATGGATG ACCTGTTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCAACTGGT TCGACTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTT GTCAGCAGGA TGAAAACTGT GCAGTTTTTA
    TCTCATGCTG TGGAAAATCC CAGAAATATT ATTCCAATGA GATTTGTAGC TGTATCTGCA
    ACAATTCCAA ATGCTGAGGA TATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCCGCTATC
    TGTCTGAAAA TGGATGAGAA ACATAGACCA GTGAAGCTTC GGAAAGTGGT CCTTGGTTTT
    CCCTGCAGTA ATAACCAAAC TGAATTTAAG TTTGATTTAA CCCTCAACTA CAAAATTGCC
    AGTGTTGTAC AAACGTACTC CGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTACAAC AGGCTGCTTC CATTCTTGTG AAAGATGCCA AGTTTATTAT GACGGTGGAA
    CAGAAACAGA GGTTACATAA ATGTGCATAT TCCATAAGAG ATTCAAAATT GAGAGATCTC
    TTAATACACG GTGTTGCTTA TCATCATGCT GGCATGGAGC TGTCAGATAG AAAAGTAGTC
    GAAGGAGCTT TTACTGTTGG AGATCTACCA GTTCTTTTTA CTACTAGTAC CTTAGCTATG
    GGAGTAAATT TGCCTGCTCA TCTAGTAGTT ATCAAATCTA CAATGCATTA TGCTGGAGGG
    ATGTTTGAAG AGTACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCAAC TGCAGTTATT ATGACTCGGT TAAGTACAAA AGAGAAGTAT
    ATTCAGATGC TGGCTTGTAG TGACACTGTA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT GTTGCATACC ATCACAGATG TCAACATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTGTA TATCAGAGCC TTGAAGAATC CGTCTCACTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATTTG
    AATGATTTAT CATCTCTGGA CTTGATAAAG ATGGATGAAG ATATTAATTT CAAGCCAACT
    GAGGCAGGAA GATTGATGGC TTGGTACTAT GTTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATCAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG GATAAATGAA AAGAAAACAC TGAATACTTT GAACAAAGAT
    CCAAATCGGA TAACTATCAG ATTTCCAATG GAAGGAAAAA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCA TACAAGACTT TGCTTTGACA
    CAAGATACCT CAAAGATTTT CAGGAATGGC TCACGAATTA CGAGATGGTT ATCAGATTTT
    GTGGCTGTTC AAGAAAAGTT TGCTGTGCTT TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCTGCAT GTATCCAAAC AACTGGAAAA AATCGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AGCTTGAACT GATTTTAAAT AGACATCCCC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTGTCTGCC AAAATACGAA CTTGAAGTGG AACAGATTGC AAGATACAGT
    GATACCACGG CAGAAGTTTT AGTGACTGTT ATATTAAGAA ACTTCGAACC ACTGCAAACT
    AAAAGAACAG CACCAGATTC TCACTATGTT ACCTTAATCA TAGGCGATGC AGATAATCAG
    CTGATTTTTA AGCATAAAAT TATGGATTCT GTTTTGCTGA AATCTGGAAA TTGGGCTAAA
    AAGATCGATG TGAAGAGAGC TCTGAAATCT GAAGATCTTA GCATAAATCT CATTAGTTCT
    GAGTATGTTG GACTTGATAT TCAGCAGAAA TTTACAGTCT TTTACTTAGG ACCCAAGAGA
    TTTGGAAATC AAATAATTAT GCAAAAAAAA TCAGAAACAG AGATCTTCCA TTCTAAACGT
    TCAGATGGAT CTCCTGTAGC AGGTCCCATT AAAGGAATGA CTGCCTGCAG AAAGCCTGGG
    CACCGAGAGT GTAACCATCA CTGTAAAAAG AAACACACGT GTGGACATGA CTGCTGTAAA
    ACTGGAGTTG CCCAGAAGCC AGAAATGAAA GAGTCAACAA TTTCTTCTTA TTTATCTGAT
    TTAAGAAACA GGAATGCAGT TTCATCTCTT CCTCCAGTTA AACGTCTAAA GATGCAGATG
    AATAAATCTC AAAGTGTGGA CCTTAAAGAG TTTGGTTTTA CTCCACAACC TTCTCTTCCC
    AGTCTGTCAA GGTCAGAATG TTTAAACATA CCTAAATTGC CTGTAATGGA GCAGAGGGGT
    CAGCACGAGC TCTATGCAGA AGGTCAACAA CAAGAACCAC CGGGATATCA GGACAAAGGT
    TTTTCCAGAC AGACCTCTTC TACAACCTCA AATGAGAAAA CCCCTCAAAC AACACTGTTC
    AACAGAAATA TTAAGGCAAC TGGAAACCAG AATTTGACAG CTGTGGTTAA AAGTAATTCT
    TCCTATTCAG AAGTTTTGAA TGTGAACTTT GAATTGGGAA ATGAAGTTTG GGATGATTTT
    GATGATGAAA ACTTAATAGA AATTGCTAGC TTTTCAGCTG ATACTGAAAA GACAAAAGCA
    GGATTTGGAA ACACTTTGAG TTCAAGTGCC AGGGAAAATA AGCTACCCTT CCAAGAATCA
    AAAAGCAAAT TCCAAAGAAA AATGTCAAAC AGTTTTGTTT CATCACATGA GAAGCCAGAT
    ATTTATTTAC CAAATTCTGC TCTGTCCAGG ATCGGTGCAT CCTCCATGAC AAAAATACCT
    CAACCAGCTG GAAATGTCCC TTTCCAAGAA GTAAAACAAC AGAATCTGTC ACCAGAGATT
    GAAAAGCTAT GCTTTGCTCA CTCTGAAAAA ATATCAAGCT TTTCAAATTC TGTTACGCAA
    GTGGATTGCT TTATTAGAAA CAGTGAATAT AAAAAGCAAC CTGACTTCAG TAAGTTATAT
    TTCTTGCCTC GTCATAAGAA ACAATATGGA TTATACGTTA AATATACAAG CTGCCAGACT
    TCATCCCAGA GCTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe248242
    Clone ID Related Accession (Same CDS sequence) XM_021090254.1 , XM_021090252.1 , XM_021090255.1 , XM_021090251.1 , XM_021090253.1
    Accession Version XM_021090255.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4458bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product probable ATP-dependent DNA helicase HFM1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010446.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCTGAGAT CACATGACTC CTTGTGTTCT TTGGATAATT TATTTTTTGA AAAAACAGAT 
    GAAGCTGAAA AATACCCAGA CAATGAAAAA TCATTAGATT GGTTTCTCCC ACCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAAGCA TTAGAGGAAG ACATGCAAAG TCATAAACTG
    TTAGGTCAGA AAAAGAGGTC AAAAATGTTA ACATCCAATT TAAAGATAAC TAATGAAGAC
    ACAAGTTATA CTTCACCAAC ACAAAAATTC CAGTGTGCTT TCCCTTCTAA TAAATACGAA
    GAAGGCGATC TAAATTTAGT AGGGGCAGGT AGCAATGACT TACATGTTGC TGGCAAGCTG
    ACTTATGGTT CTCCTCAGAA GTACAAAAGT CACATTGGCC CTGAGATAGC ACTTGAGAAA
    AATGTTCCAG ATGATGCGAA AGTAGTTAAT TTTGCATACG ATAAAGGAGA GAGCACATCG
    GCCTTCCGAA AAAGATTATT TAAAATATCT GATAGTATAC ATGGGAATGC TTGTTCTAAT
    GACATTGCAA ACTTGGACTC TGATATTGGC TCAGCGAAGA TTGCCCAAAT GGAAATGAGC
    GAAGGGAAAT CGTGGAAATG GAACAATAGT AATCAGAAAC CTCAATATTC AGAAATTAAC
    ATGTTTACAG CCAGTGATAC TTTTTCTGCT TCTAAAATCA GAGAAGGCAT ATTCAAAGCA
    CCATCTTTTT CAGTTGCATC CCAGCCTCAT GAAGTTCAAG GGATAATAGA AAATGGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATCCCG GCAAAATTTA GAAGTGTTTT CAAAGAATTC
    CCATATTTCA ACTATATCCA GTCTAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    GTTGTGGTTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAA TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGCAGTCA GCGTTTTGAT GACTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC GGGAGATACA GTCATGGATG ACCTGTTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCAACTGGT TCGACTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTT GTCAGCAGGA TGAAAACTGT GCAGTTTTTA
    TCTCATGCTG TGGAAAATCC CAGAAATATT ATTCCAATGA GATTTGTAGC TGTATCTGCA
    ACAATTCCAA ATGCTGAGGA TATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCCGCTATC
    TGTCTGAAAA TGGATGAGAA ACATAGACCA GTGAAGCTTC GGAAAGTGGT CCTTGGTTTT
    CCCTGCAGTA ATAACCAAAC TGAATTTAAG TTTGATTTAA CCCTCAACTA CAAAATTGCC
    AGTGTTGTAC AAACGTACTC CGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTACAAC AGGCTGCTTC CATTCTTGTG AAAGATGCCA AGTTTATTAT GACGGTGGAA
    CAGAAACAGA GGTTACATAA ATGTGCATAT TCCATAAGAG ATTCAAAATT GAGAGATCTC
    TTAATACACG GTGTTGCTTA TCATCATGCT GGCATGGAGC TGTCAGATAG AAAAGTAGTC
    GAAGGAGCTT TTACTGTTGG AGATCTACCA GTTCTTTTTA CTACTAGTAC CTTAGCTATG
    GGAGTAAATT TGCCTGCTCA TCTAGTAGTT ATCAAATCTA CAATGCATTA TGCTGGAGGG
    ATGTTTGAAG AGTACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCAAC TGCAGTTATT ATGACTCGGT TAAGTACAAA AGAGAAGTAT
    ATTCAGATGC TGGCTTGTAG TGACACTGTA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT GTTGCATACC ATCACAGATG TCAACATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTGTA TATCAGAGCC TTGAAGAATC CGTCTCACTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATTTG
    AATGATTTAT CATCTCTGGA CTTGATAAAG ATGGATGAAG ATATTAATTT CAAGCCAACT
    GAGGCAGGAA GATTGATGGC TTGGTACTAT GTTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATCAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG GATAAATGAA AAGAAAACAC TGAATACTTT GAACAAAGAT
    CCAAATCGGA TAACTATCAG ATTTCCAATG GAAGGAAAAA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCA TACAAGACTT TGCTTTGACA
    CAAGATACCT CAAAGATTTT CAGGAATGGC TCACGAATTA CGAGATGGTT ATCAGATTTT
    GTGGCTGTTC AAGAAAAGTT TGCTGTGCTT TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCTGCAT GTATCCAAAC AACTGGAAAA AATCGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AGCTTGAACT GATTTTAAAT AGACATCCCC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTGTCTGCC AAAATACGAA CTTGAAGTGG AACAGATTGC AAGATACAGT
    GATACCACGG CAGAAGTTTT AGTGACTGTT ATATTAAGAA ACTTCGAACC ACTGCAAACT
    AAAAGAACAG CACCAGATTC TCACTATGTT ACCTTAATCA TAGGCGATGC AGATAATCAG
    CTGATTTTTA AGCATAAAAT TATGGATTCT GTTTTGCTGA AATCTGGAAA TTGGGCTAAA
    AAGATCGATG TGAAGAGAGC TCTGAAATCT GAAGATCTTA GCATAAATCT CATTAGTTCT
    GAGTATGTTG GACTTGATAT TCAGCAGAAA TTTACAGTCT TTTACTTAGG ACCCAAGAGA
    TTTGGAAATC AAATAATTAT GCAAAAAAAA TCAGAAACAG AGATCTTCCA TTCTAAACGT
    TCAGATGGAT CTCCTGTAGC AGGTCCCATT AAAGGAATGA CTGCCTGCAG AAAGCCTGGG
    CACCGAGAGT GTAACCATCA CTGTAAAAAG AAACACACGT GTGGACATGA CTGCTGTAAA
    ACTGGAGTTG CCCAGAAGCC AGAAATGAAA GAGTCAACAA TTTCTTCTTA TTTATCTGAT
    TTAAGAAACA GGAATGCAGT TTCATCTCTT CCTCCAGTTA AACGTCTAAA GATGCAGATG
    AATAAATCTC AAAGTGTGGA CCTTAAAGAG TTTGGTTTTA CTCCACAACC TTCTCTTCCC
    AGTCTGTCAA GGTCAGAATG TTTAAACATA CCTAAATTGC CTGTAATGGA GCAGAGGGGT
    CAGCACGAGC TCTATGCAGA AGGTCAACAA CAAGAACCAC CGGGATATCA GGACAAAGGT
    TTTTCCAGAC AGACCTCTTC TACAACCTCA AATGAGAAAA CCCCTCAAAC AACACTGTTC
    AACAGAAATA TTAAGGCAAC TGGAAACCAG AATTTGACAG CTGTGGTTAA AAGTAATTCT
    TCCTATTCAG AAGTTTTGAA TGTGAACTTT GAATTGGGAA ATGAAGTTTG GGATGATTTT
    GATGATGAAA ACTTAATAGA AATTGCTAGC TTTTCAGCTG ATACTGAAAA GACAAAAGCA
    GGATTTGGAA ACACTTTGAG TTCAAGTGCC AGGGAAAATA AGCTACCCTT CCAAGAATCA
    AAAAGCAAAT TCCAAAGAAA AATGTCAAAC AGTTTTGTTT CATCACATGA GAAGCCAGAT
    ATTTATTTAC CAAATTCTGC TCTGTCCAGG ATCGGTGCAT CCTCCATGAC AAAAATACCT
    CAACCAGCTG GAAATGTCCC TTTCCAAGAA GTAAAACAAC AGAATCTGTC ACCAGAGATT
    GAAAAGCTAT GCTTTGCTCA CTCTGAAAAA ATATCAAGCT TTTCAAATTC TGTTACGCAA
    GTGGATTGCT TTATTAGAAA CAGTGAATAT AAAAAGCAAC CTGACTTCAG TAAGTTATAT
    TTCTTGCCTC GTCATAAGAA ACAATATGGA TTATACGTTA AATATACAAG CTGCCAGACT
    TCATCCCAGA GCTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020945914.1
    CDS525..4982
    Translation

    Target ORF information:

    RefSeq Version XM_021090255.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021090255.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCTGAGAT CACATGACTC CTTGTGTTCT TTGGATAATT TATTTTTTGA AAAAACAGAT 
    GAAGCTGAAA AATACCCAGA CAATGAAAAA TCATTAGATT GGTTTCTCCC ACCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAAGCA TTAGAGGAAG ACATGCAAAG TCATAAACTG
    TTAGGTCAGA AAAAGAGGTC AAAAATGTTA ACATCCAATT TAAAGATAAC TAATGAAGAC
    ACAAGTTATA CTTCACCAAC ACAAAAATTC CAGTGTGCTT TCCCTTCTAA TAAATACGAA
    GAAGGCGATC TAAATTTAGT AGGGGCAGGT AGCAATGACT TACATGTTGC TGGCAAGCTG
    ACTTATGGTT CTCCTCAGAA GTACAAAAGT CACATTGGCC CTGAGATAGC ACTTGAGAAA
    AATGTTCCAG ATGATGCGAA AGTAGTTAAT TTTGCATACG ATAAAGGAGA GAGCACATCG
    GCCTTCCGAA AAAGATTATT TAAAATATCT GATAGTATAC ATGGGAATGC TTGTTCTAAT
    GACATTGCAA ACTTGGACTC TGATATTGGC TCAGCGAAGA TTGCCCAAAT GGAAATGAGC
    GAAGGGAAAT CGTGGAAATG GAACAATAGT AATCAGAAAC CTCAATATTC AGAAATTAAC
    ATGTTTACAG CCAGTGATAC TTTTTCTGCT TCTAAAATCA GAGAAGGCAT ATTCAAAGCA
    CCATCTTTTT CAGTTGCATC CCAGCCTCAT GAAGTTCAAG GGATAATAGA AAATGGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATCCCG GCAAAATTTA GAAGTGTTTT CAAAGAATTC
    CCATATTTCA ACTATATCCA GTCTAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    GTTGTGGTTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAA TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGCAGTCA GCGTTTTGAT GACTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC GGGAGATACA GTCATGGATG ACCTGTTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCAACTGGT TCGACTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTT GTCAGCAGGA TGAAAACTGT GCAGTTTTTA
    TCTCATGCTG TGGAAAATCC CAGAAATATT ATTCCAATGA GATTTGTAGC TGTATCTGCA
    ACAATTCCAA ATGCTGAGGA TATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCCGCTATC
    TGTCTGAAAA TGGATGAGAA ACATAGACCA GTGAAGCTTC GGAAAGTGGT CCTTGGTTTT
    CCCTGCAGTA ATAACCAAAC TGAATTTAAG TTTGATTTAA CCCTCAACTA CAAAATTGCC
    AGTGTTGTAC AAACGTACTC CGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTACAAC AGGCTGCTTC CATTCTTGTG AAAGATGCCA AGTTTATTAT GACGGTGGAA
    CAGAAACAGA GGTTACATAA ATGTGCATAT TCCATAAGAG ATTCAAAATT GAGAGATCTC
    TTAATACACG GTGTTGCTTA TCATCATGCT GGCATGGAGC TGTCAGATAG AAAAGTAGTC
    GAAGGAGCTT TTACTGTTGG AGATCTACCA GTTCTTTTTA CTACTAGTAC CTTAGCTATG
    GGAGTAAATT TGCCTGCTCA TCTAGTAGTT ATCAAATCTA CAATGCATTA TGCTGGAGGG
    ATGTTTGAAG AGTACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCAAC TGCAGTTATT ATGACTCGGT TAAGTACAAA AGAGAAGTAT
    ATTCAGATGC TGGCTTGTAG TGACACTGTA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT GTTGCATACC ATCACAGATG TCAACATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTGTA TATCAGAGCC TTGAAGAATC CGTCTCACTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATTTG
    AATGATTTAT CATCTCTGGA CTTGATAAAG ATGGATGAAG ATATTAATTT CAAGCCAACT
    GAGGCAGGAA GATTGATGGC TTGGTACTAT GTTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATCAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG GATAAATGAA AAGAAAACAC TGAATACTTT GAACAAAGAT
    CCAAATCGGA TAACTATCAG ATTTCCAATG GAAGGAAAAA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCA TACAAGACTT TGCTTTGACA
    CAAGATACCT CAAAGATTTT CAGGAATGGC TCACGAATTA CGAGATGGTT ATCAGATTTT
    GTGGCTGTTC AAGAAAAGTT TGCTGTGCTT TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCTGCAT GTATCCAAAC AACTGGAAAA AATCGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AGCTTGAACT GATTTTAAAT AGACATCCCC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTGTCTGCC AAAATACGAA CTTGAAGTGG AACAGATTGC AAGATACAGT
    GATACCACGG CAGAAGTTTT AGTGACTGTT ATATTAAGAA ACTTCGAACC ACTGCAAACT
    AAAAGAACAG CACCAGATTC TCACTATGTT ACCTTAATCA TAGGCGATGC AGATAATCAG
    CTGATTTTTA AGCATAAAAT TATGGATTCT GTTTTGCTGA AATCTGGAAA TTGGGCTAAA
    AAGATCGATG TGAAGAGAGC TCTGAAATCT GAAGATCTTA GCATAAATCT CATTAGTTCT
    GAGTATGTTG GACTTGATAT TCAGCAGAAA TTTACAGTCT TTTACTTAGG ACCCAAGAGA
    TTTGGAAATC AAATAATTAT GCAAAAAAAA TCAGAAACAG AGATCTTCCA TTCTAAACGT
    TCAGATGGAT CTCCTGTAGC AGGTCCCATT AAAGGAATGA CTGCCTGCAG AAAGCCTGGG
    CACCGAGAGT GTAACCATCA CTGTAAAAAG AAACACACGT GTGGACATGA CTGCTGTAAA
    ACTGGAGTTG CCCAGAAGCC AGAAATGAAA GAGTCAACAA TTTCTTCTTA TTTATCTGAT
    TTAAGAAACA GGAATGCAGT TTCATCTCTT CCTCCAGTTA AACGTCTAAA GATGCAGATG
    AATAAATCTC AAAGTGTGGA CCTTAAAGAG TTTGGTTTTA CTCCACAACC TTCTCTTCCC
    AGTCTGTCAA GGTCAGAATG TTTAAACATA CCTAAATTGC CTGTAATGGA GCAGAGGGGT
    CAGCACGAGC TCTATGCAGA AGGTCAACAA CAAGAACCAC CGGGATATCA GGACAAAGGT
    TTTTCCAGAC AGACCTCTTC TACAACCTCA AATGAGAAAA CCCCTCAAAC AACACTGTTC
    AACAGAAATA TTAAGGCAAC TGGAAACCAG AATTTGACAG CTGTGGTTAA AAGTAATTCT
    TCCTATTCAG AAGTTTTGAA TGTGAACTTT GAATTGGGAA ATGAAGTTTG GGATGATTTT
    GATGATGAAA ACTTAATAGA AATTGCTAGC TTTTCAGCTG ATACTGAAAA GACAAAAGCA
    GGATTTGGAA ACACTTTGAG TTCAAGTGCC AGGGAAAATA AGCTACCCTT CCAAGAATCA
    AAAAGCAAAT TCCAAAGAAA AATGTCAAAC AGTTTTGTTT CATCACATGA GAAGCCAGAT
    ATTTATTTAC CAAATTCTGC TCTGTCCAGG ATCGGTGCAT CCTCCATGAC AAAAATACCT
    CAACCAGCTG GAAATGTCCC TTTCCAAGAA GTAAAACAAC AGAATCTGTC ACCAGAGATT
    GAAAAGCTAT GCTTTGCTCA CTCTGAAAAA ATATCAAGCT TTTCAAATTC TGTTACGCAA
    GTGGATTGCT TTATTAGAAA CAGTGAATAT AAAAAGCAAC CTGACTTCAG TAAGTTATAT
    TTCTTGCCTC GTCATAAGAA ACAATATGGA TTATACGTTA AATATACAAG CTGCCAGACT
    TCATCCCAGA GCTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe248242
    Clone ID Related Accession (Same CDS sequence) XM_021090254.1 , XM_021090252.1 , XM_021090255.1 , XM_021090251.1 , XM_021090253.1
    Accession Version XM_021090251.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4458bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product probable ATP-dependent DNA helicase HFM1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010446.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCTGAGAT CACATGACTC CTTGTGTTCT TTGGATAATT TATTTTTTGA AAAAACAGAT 
    GAAGCTGAAA AATACCCAGA CAATGAAAAA TCATTAGATT GGTTTCTCCC ACCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAAGCA TTAGAGGAAG ACATGCAAAG TCATAAACTG
    TTAGGTCAGA AAAAGAGGTC AAAAATGTTA ACATCCAATT TAAAGATAAC TAATGAAGAC
    ACAAGTTATA CTTCACCAAC ACAAAAATTC CAGTGTGCTT TCCCTTCTAA TAAATACGAA
    GAAGGCGATC TAAATTTAGT AGGGGCAGGT AGCAATGACT TACATGTTGC TGGCAAGCTG
    ACTTATGGTT CTCCTCAGAA GTACAAAAGT CACATTGGCC CTGAGATAGC ACTTGAGAAA
    AATGTTCCAG ATGATGCGAA AGTAGTTAAT TTTGCATACG ATAAAGGAGA GAGCACATCG
    GCCTTCCGAA AAAGATTATT TAAAATATCT GATAGTATAC ATGGGAATGC TTGTTCTAAT
    GACATTGCAA ACTTGGACTC TGATATTGGC TCAGCGAAGA TTGCCCAAAT GGAAATGAGC
    GAAGGGAAAT CGTGGAAATG GAACAATAGT AATCAGAAAC CTCAATATTC AGAAATTAAC
    ATGTTTACAG CCAGTGATAC TTTTTCTGCT TCTAAAATCA GAGAAGGCAT ATTCAAAGCA
    CCATCTTTTT CAGTTGCATC CCAGCCTCAT GAAGTTCAAG GGATAATAGA AAATGGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATCCCG GCAAAATTTA GAAGTGTTTT CAAAGAATTC
    CCATATTTCA ACTATATCCA GTCTAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    GTTGTGGTTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAA TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGCAGTCA GCGTTTTGAT GACTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC GGGAGATACA GTCATGGATG ACCTGTTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCAACTGGT TCGACTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTT GTCAGCAGGA TGAAAACTGT GCAGTTTTTA
    TCTCATGCTG TGGAAAATCC CAGAAATATT ATTCCAATGA GATTTGTAGC TGTATCTGCA
    ACAATTCCAA ATGCTGAGGA TATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCCGCTATC
    TGTCTGAAAA TGGATGAGAA ACATAGACCA GTGAAGCTTC GGAAAGTGGT CCTTGGTTTT
    CCCTGCAGTA ATAACCAAAC TGAATTTAAG TTTGATTTAA CCCTCAACTA CAAAATTGCC
    AGTGTTGTAC AAACGTACTC CGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTACAAC AGGCTGCTTC CATTCTTGTG AAAGATGCCA AGTTTATTAT GACGGTGGAA
    CAGAAACAGA GGTTACATAA ATGTGCATAT TCCATAAGAG ATTCAAAATT GAGAGATCTC
    TTAATACACG GTGTTGCTTA TCATCATGCT GGCATGGAGC TGTCAGATAG AAAAGTAGTC
    GAAGGAGCTT TTACTGTTGG AGATCTACCA GTTCTTTTTA CTACTAGTAC CTTAGCTATG
    GGAGTAAATT TGCCTGCTCA TCTAGTAGTT ATCAAATCTA CAATGCATTA TGCTGGAGGG
    ATGTTTGAAG AGTACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCAAC TGCAGTTATT ATGACTCGGT TAAGTACAAA AGAGAAGTAT
    ATTCAGATGC TGGCTTGTAG TGACACTGTA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT GTTGCATACC ATCACAGATG TCAACATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTGTA TATCAGAGCC TTGAAGAATC CGTCTCACTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATTTG
    AATGATTTAT CATCTCTGGA CTTGATAAAG ATGGATGAAG ATATTAATTT CAAGCCAACT
    GAGGCAGGAA GATTGATGGC TTGGTACTAT GTTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATCAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG GATAAATGAA AAGAAAACAC TGAATACTTT GAACAAAGAT
    CCAAATCGGA TAACTATCAG ATTTCCAATG GAAGGAAAAA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCA TACAAGACTT TGCTTTGACA
    CAAGATACCT CAAAGATTTT CAGGAATGGC TCACGAATTA CGAGATGGTT ATCAGATTTT
    GTGGCTGTTC AAGAAAAGTT TGCTGTGCTT TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCTGCAT GTATCCAAAC AACTGGAAAA AATCGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AGCTTGAACT GATTTTAAAT AGACATCCCC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTGTCTGCC AAAATACGAA CTTGAAGTGG AACAGATTGC AAGATACAGT
    GATACCACGG CAGAAGTTTT AGTGACTGTT ATATTAAGAA ACTTCGAACC ACTGCAAACT
    AAAAGAACAG CACCAGATTC TCACTATGTT ACCTTAATCA TAGGCGATGC AGATAATCAG
    CTGATTTTTA AGCATAAAAT TATGGATTCT GTTTTGCTGA AATCTGGAAA TTGGGCTAAA
    AAGATCGATG TGAAGAGAGC TCTGAAATCT GAAGATCTTA GCATAAATCT CATTAGTTCT
    GAGTATGTTG GACTTGATAT TCAGCAGAAA TTTACAGTCT TTTACTTAGG ACCCAAGAGA
    TTTGGAAATC AAATAATTAT GCAAAAAAAA TCAGAAACAG AGATCTTCCA TTCTAAACGT
    TCAGATGGAT CTCCTGTAGC AGGTCCCATT AAAGGAATGA CTGCCTGCAG AAAGCCTGGG
    CACCGAGAGT GTAACCATCA CTGTAAAAAG AAACACACGT GTGGACATGA CTGCTGTAAA
    ACTGGAGTTG CCCAGAAGCC AGAAATGAAA GAGTCAACAA TTTCTTCTTA TTTATCTGAT
    TTAAGAAACA GGAATGCAGT TTCATCTCTT CCTCCAGTTA AACGTCTAAA GATGCAGATG
    AATAAATCTC AAAGTGTGGA CCTTAAAGAG TTTGGTTTTA CTCCACAACC TTCTCTTCCC
    AGTCTGTCAA GGTCAGAATG TTTAAACATA CCTAAATTGC CTGTAATGGA GCAGAGGGGT
    CAGCACGAGC TCTATGCAGA AGGTCAACAA CAAGAACCAC CGGGATATCA GGACAAAGGT
    TTTTCCAGAC AGACCTCTTC TACAACCTCA AATGAGAAAA CCCCTCAAAC AACACTGTTC
    AACAGAAATA TTAAGGCAAC TGGAAACCAG AATTTGACAG CTGTGGTTAA AAGTAATTCT
    TCCTATTCAG AAGTTTTGAA TGTGAACTTT GAATTGGGAA ATGAAGTTTG GGATGATTTT
    GATGATGAAA ACTTAATAGA AATTGCTAGC TTTTCAGCTG ATACTGAAAA GACAAAAGCA
    GGATTTGGAA ACACTTTGAG TTCAAGTGCC AGGGAAAATA AGCTACCCTT CCAAGAATCA
    AAAAGCAAAT TCCAAAGAAA AATGTCAAAC AGTTTTGTTT CATCACATGA GAAGCCAGAT
    ATTTATTTAC CAAATTCTGC TCTGTCCAGG ATCGGTGCAT CCTCCATGAC AAAAATACCT
    CAACCAGCTG GAAATGTCCC TTTCCAAGAA GTAAAACAAC AGAATCTGTC ACCAGAGATT
    GAAAAGCTAT GCTTTGCTCA CTCTGAAAAA ATATCAAGCT TTTCAAATTC TGTTACGCAA
    GTGGATTGCT TTATTAGAAA CAGTGAATAT AAAAAGCAAC CTGACTTCAG TAAGTTATAT
    TTCTTGCCTC GTCATAAGAA ACAATATGGA TTATACGTTA AATATACAAG CTGCCAGACT
    TCATCCCAGA GCTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020945910.1
    CDS103..4560
    Translation

    Target ORF information:

    RefSeq Version XM_021090251.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021090251.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCTGAGAT CACATGACTC CTTGTGTTCT TTGGATAATT TATTTTTTGA AAAAACAGAT 
    GAAGCTGAAA AATACCCAGA CAATGAAAAA TCATTAGATT GGTTTCTCCC ACCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAAGCA TTAGAGGAAG ACATGCAAAG TCATAAACTG
    TTAGGTCAGA AAAAGAGGTC AAAAATGTTA ACATCCAATT TAAAGATAAC TAATGAAGAC
    ACAAGTTATA CTTCACCAAC ACAAAAATTC CAGTGTGCTT TCCCTTCTAA TAAATACGAA
    GAAGGCGATC TAAATTTAGT AGGGGCAGGT AGCAATGACT TACATGTTGC TGGCAAGCTG
    ACTTATGGTT CTCCTCAGAA GTACAAAAGT CACATTGGCC CTGAGATAGC ACTTGAGAAA
    AATGTTCCAG ATGATGCGAA AGTAGTTAAT TTTGCATACG ATAAAGGAGA GAGCACATCG
    GCCTTCCGAA AAAGATTATT TAAAATATCT GATAGTATAC ATGGGAATGC TTGTTCTAAT
    GACATTGCAA ACTTGGACTC TGATATTGGC TCAGCGAAGA TTGCCCAAAT GGAAATGAGC
    GAAGGGAAAT CGTGGAAATG GAACAATAGT AATCAGAAAC CTCAATATTC AGAAATTAAC
    ATGTTTACAG CCAGTGATAC TTTTTCTGCT TCTAAAATCA GAGAAGGCAT ATTCAAAGCA
    CCATCTTTTT CAGTTGCATC CCAGCCTCAT GAAGTTCAAG GGATAATAGA AAATGGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATCCCG GCAAAATTTA GAAGTGTTTT CAAAGAATTC
    CCATATTTCA ACTATATCCA GTCTAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    GTTGTGGTTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAA TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGCAGTCA GCGTTTTGAT GACTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC GGGAGATACA GTCATGGATG ACCTGTTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCAACTGGT TCGACTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTT GTCAGCAGGA TGAAAACTGT GCAGTTTTTA
    TCTCATGCTG TGGAAAATCC CAGAAATATT ATTCCAATGA GATTTGTAGC TGTATCTGCA
    ACAATTCCAA ATGCTGAGGA TATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCCGCTATC
    TGTCTGAAAA TGGATGAGAA ACATAGACCA GTGAAGCTTC GGAAAGTGGT CCTTGGTTTT
    CCCTGCAGTA ATAACCAAAC TGAATTTAAG TTTGATTTAA CCCTCAACTA CAAAATTGCC
    AGTGTTGTAC AAACGTACTC CGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTACAAC AGGCTGCTTC CATTCTTGTG AAAGATGCCA AGTTTATTAT GACGGTGGAA
    CAGAAACAGA GGTTACATAA ATGTGCATAT TCCATAAGAG ATTCAAAATT GAGAGATCTC
    TTAATACACG GTGTTGCTTA TCATCATGCT GGCATGGAGC TGTCAGATAG AAAAGTAGTC
    GAAGGAGCTT TTACTGTTGG AGATCTACCA GTTCTTTTTA CTACTAGTAC CTTAGCTATG
    GGAGTAAATT TGCCTGCTCA TCTAGTAGTT ATCAAATCTA CAATGCATTA TGCTGGAGGG
    ATGTTTGAAG AGTACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCAAC TGCAGTTATT ATGACTCGGT TAAGTACAAA AGAGAAGTAT
    ATTCAGATGC TGGCTTGTAG TGACACTGTA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT GTTGCATACC ATCACAGATG TCAACATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTGTA TATCAGAGCC TTGAAGAATC CGTCTCACTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATTTG
    AATGATTTAT CATCTCTGGA CTTGATAAAG ATGGATGAAG ATATTAATTT CAAGCCAACT
    GAGGCAGGAA GATTGATGGC TTGGTACTAT GTTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATCAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG GATAAATGAA AAGAAAACAC TGAATACTTT GAACAAAGAT
    CCAAATCGGA TAACTATCAG ATTTCCAATG GAAGGAAAAA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCA TACAAGACTT TGCTTTGACA
    CAAGATACCT CAAAGATTTT CAGGAATGGC TCACGAATTA CGAGATGGTT ATCAGATTTT
    GTGGCTGTTC AAGAAAAGTT TGCTGTGCTT TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCTGCAT GTATCCAAAC AACTGGAAAA AATCGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AGCTTGAACT GATTTTAAAT AGACATCCCC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTGTCTGCC AAAATACGAA CTTGAAGTGG AACAGATTGC AAGATACAGT
    GATACCACGG CAGAAGTTTT AGTGACTGTT ATATTAAGAA ACTTCGAACC ACTGCAAACT
    AAAAGAACAG CACCAGATTC TCACTATGTT ACCTTAATCA TAGGCGATGC AGATAATCAG
    CTGATTTTTA AGCATAAAAT TATGGATTCT GTTTTGCTGA AATCTGGAAA TTGGGCTAAA
    AAGATCGATG TGAAGAGAGC TCTGAAATCT GAAGATCTTA GCATAAATCT CATTAGTTCT
    GAGTATGTTG GACTTGATAT TCAGCAGAAA TTTACAGTCT TTTACTTAGG ACCCAAGAGA
    TTTGGAAATC AAATAATTAT GCAAAAAAAA TCAGAAACAG AGATCTTCCA TTCTAAACGT
    TCAGATGGAT CTCCTGTAGC AGGTCCCATT AAAGGAATGA CTGCCTGCAG AAAGCCTGGG
    CACCGAGAGT GTAACCATCA CTGTAAAAAG AAACACACGT GTGGACATGA CTGCTGTAAA
    ACTGGAGTTG CCCAGAAGCC AGAAATGAAA GAGTCAACAA TTTCTTCTTA TTTATCTGAT
    TTAAGAAACA GGAATGCAGT TTCATCTCTT CCTCCAGTTA AACGTCTAAA GATGCAGATG
    AATAAATCTC AAAGTGTGGA CCTTAAAGAG TTTGGTTTTA CTCCACAACC TTCTCTTCCC
    AGTCTGTCAA GGTCAGAATG TTTAAACATA CCTAAATTGC CTGTAATGGA GCAGAGGGGT
    CAGCACGAGC TCTATGCAGA AGGTCAACAA CAAGAACCAC CGGGATATCA GGACAAAGGT
    TTTTCCAGAC AGACCTCTTC TACAACCTCA AATGAGAAAA CCCCTCAAAC AACACTGTTC
    AACAGAAATA TTAAGGCAAC TGGAAACCAG AATTTGACAG CTGTGGTTAA AAGTAATTCT
    TCCTATTCAG AAGTTTTGAA TGTGAACTTT GAATTGGGAA ATGAAGTTTG GGATGATTTT
    GATGATGAAA ACTTAATAGA AATTGCTAGC TTTTCAGCTG ATACTGAAAA GACAAAAGCA
    GGATTTGGAA ACACTTTGAG TTCAAGTGCC AGGGAAAATA AGCTACCCTT CCAAGAATCA
    AAAAGCAAAT TCCAAAGAAA AATGTCAAAC AGTTTTGTTT CATCACATGA GAAGCCAGAT
    ATTTATTTAC CAAATTCTGC TCTGTCCAGG ATCGGTGCAT CCTCCATGAC AAAAATACCT
    CAACCAGCTG GAAATGTCCC TTTCCAAGAA GTAAAACAAC AGAATCTGTC ACCAGAGATT
    GAAAAGCTAT GCTTTGCTCA CTCTGAAAAA ATATCAAGCT TTTCAAATTC TGTTACGCAA
    GTGGATTGCT TTATTAGAAA CAGTGAATAT AAAAAGCAAC CTGACTTCAG TAAGTTATAT
    TTCTTGCCTC GTCATAAGAA ACAATATGGA TTATACGTTA AATATACAAG CTGCCAGACT
    TCATCCCAGA GCTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe248242
    Clone ID Related Accession (Same CDS sequence) XM_021090254.1 , XM_021090252.1 , XM_021090255.1 , XM_021090251.1 , XM_021090253.1
    Accession Version XM_021090253.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4458bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product probable ATP-dependent DNA helicase HFM1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010446.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCTGAGAT CACATGACTC CTTGTGTTCT TTGGATAATT TATTTTTTGA AAAAACAGAT 
    GAAGCTGAAA AATACCCAGA CAATGAAAAA TCATTAGATT GGTTTCTCCC ACCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAAGCA TTAGAGGAAG ACATGCAAAG TCATAAACTG
    TTAGGTCAGA AAAAGAGGTC AAAAATGTTA ACATCCAATT TAAAGATAAC TAATGAAGAC
    ACAAGTTATA CTTCACCAAC ACAAAAATTC CAGTGTGCTT TCCCTTCTAA TAAATACGAA
    GAAGGCGATC TAAATTTAGT AGGGGCAGGT AGCAATGACT TACATGTTGC TGGCAAGCTG
    ACTTATGGTT CTCCTCAGAA GTACAAAAGT CACATTGGCC CTGAGATAGC ACTTGAGAAA
    AATGTTCCAG ATGATGCGAA AGTAGTTAAT TTTGCATACG ATAAAGGAGA GAGCACATCG
    GCCTTCCGAA AAAGATTATT TAAAATATCT GATAGTATAC ATGGGAATGC TTGTTCTAAT
    GACATTGCAA ACTTGGACTC TGATATTGGC TCAGCGAAGA TTGCCCAAAT GGAAATGAGC
    GAAGGGAAAT CGTGGAAATG GAACAATAGT AATCAGAAAC CTCAATATTC AGAAATTAAC
    ATGTTTACAG CCAGTGATAC TTTTTCTGCT TCTAAAATCA GAGAAGGCAT ATTCAAAGCA
    CCATCTTTTT CAGTTGCATC CCAGCCTCAT GAAGTTCAAG GGATAATAGA AAATGGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATCCCG GCAAAATTTA GAAGTGTTTT CAAAGAATTC
    CCATATTTCA ACTATATCCA GTCTAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    GTTGTGGTTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAA TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGCAGTCA GCGTTTTGAT GACTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC GGGAGATACA GTCATGGATG ACCTGTTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCAACTGGT TCGACTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTT GTCAGCAGGA TGAAAACTGT GCAGTTTTTA
    TCTCATGCTG TGGAAAATCC CAGAAATATT ATTCCAATGA GATTTGTAGC TGTATCTGCA
    ACAATTCCAA ATGCTGAGGA TATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCCGCTATC
    TGTCTGAAAA TGGATGAGAA ACATAGACCA GTGAAGCTTC GGAAAGTGGT CCTTGGTTTT
    CCCTGCAGTA ATAACCAAAC TGAATTTAAG TTTGATTTAA CCCTCAACTA CAAAATTGCC
    AGTGTTGTAC AAACGTACTC CGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTACAAC AGGCTGCTTC CATTCTTGTG AAAGATGCCA AGTTTATTAT GACGGTGGAA
    CAGAAACAGA GGTTACATAA ATGTGCATAT TCCATAAGAG ATTCAAAATT GAGAGATCTC
    TTAATACACG GTGTTGCTTA TCATCATGCT GGCATGGAGC TGTCAGATAG AAAAGTAGTC
    GAAGGAGCTT TTACTGTTGG AGATCTACCA GTTCTTTTTA CTACTAGTAC CTTAGCTATG
    GGAGTAAATT TGCCTGCTCA TCTAGTAGTT ATCAAATCTA CAATGCATTA TGCTGGAGGG
    ATGTTTGAAG AGTACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCAAC TGCAGTTATT ATGACTCGGT TAAGTACAAA AGAGAAGTAT
    ATTCAGATGC TGGCTTGTAG TGACACTGTA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT GTTGCATACC ATCACAGATG TCAACATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTGTA TATCAGAGCC TTGAAGAATC CGTCTCACTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATTTG
    AATGATTTAT CATCTCTGGA CTTGATAAAG ATGGATGAAG ATATTAATTT CAAGCCAACT
    GAGGCAGGAA GATTGATGGC TTGGTACTAT GTTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATCAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG GATAAATGAA AAGAAAACAC TGAATACTTT GAACAAAGAT
    CCAAATCGGA TAACTATCAG ATTTCCAATG GAAGGAAAAA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCA TACAAGACTT TGCTTTGACA
    CAAGATACCT CAAAGATTTT CAGGAATGGC TCACGAATTA CGAGATGGTT ATCAGATTTT
    GTGGCTGTTC AAGAAAAGTT TGCTGTGCTT TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCTGCAT GTATCCAAAC AACTGGAAAA AATCGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AGCTTGAACT GATTTTAAAT AGACATCCCC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTGTCTGCC AAAATACGAA CTTGAAGTGG AACAGATTGC AAGATACAGT
    GATACCACGG CAGAAGTTTT AGTGACTGTT ATATTAAGAA ACTTCGAACC ACTGCAAACT
    AAAAGAACAG CACCAGATTC TCACTATGTT ACCTTAATCA TAGGCGATGC AGATAATCAG
    CTGATTTTTA AGCATAAAAT TATGGATTCT GTTTTGCTGA AATCTGGAAA TTGGGCTAAA
    AAGATCGATG TGAAGAGAGC TCTGAAATCT GAAGATCTTA GCATAAATCT CATTAGTTCT
    GAGTATGTTG GACTTGATAT TCAGCAGAAA TTTACAGTCT TTTACTTAGG ACCCAAGAGA
    TTTGGAAATC AAATAATTAT GCAAAAAAAA TCAGAAACAG AGATCTTCCA TTCTAAACGT
    TCAGATGGAT CTCCTGTAGC AGGTCCCATT AAAGGAATGA CTGCCTGCAG AAAGCCTGGG
    CACCGAGAGT GTAACCATCA CTGTAAAAAG AAACACACGT GTGGACATGA CTGCTGTAAA
    ACTGGAGTTG CCCAGAAGCC AGAAATGAAA GAGTCAACAA TTTCTTCTTA TTTATCTGAT
    TTAAGAAACA GGAATGCAGT TTCATCTCTT CCTCCAGTTA AACGTCTAAA GATGCAGATG
    AATAAATCTC AAAGTGTGGA CCTTAAAGAG TTTGGTTTTA CTCCACAACC TTCTCTTCCC
    AGTCTGTCAA GGTCAGAATG TTTAAACATA CCTAAATTGC CTGTAATGGA GCAGAGGGGT
    CAGCACGAGC TCTATGCAGA AGGTCAACAA CAAGAACCAC CGGGATATCA GGACAAAGGT
    TTTTCCAGAC AGACCTCTTC TACAACCTCA AATGAGAAAA CCCCTCAAAC AACACTGTTC
    AACAGAAATA TTAAGGCAAC TGGAAACCAG AATTTGACAG CTGTGGTTAA AAGTAATTCT
    TCCTATTCAG AAGTTTTGAA TGTGAACTTT GAATTGGGAA ATGAAGTTTG GGATGATTTT
    GATGATGAAA ACTTAATAGA AATTGCTAGC TTTTCAGCTG ATACTGAAAA GACAAAAGCA
    GGATTTGGAA ACACTTTGAG TTCAAGTGCC AGGGAAAATA AGCTACCCTT CCAAGAATCA
    AAAAGCAAAT TCCAAAGAAA AATGTCAAAC AGTTTTGTTT CATCACATGA GAAGCCAGAT
    ATTTATTTAC CAAATTCTGC TCTGTCCAGG ATCGGTGCAT CCTCCATGAC AAAAATACCT
    CAACCAGCTG GAAATGTCCC TTTCCAAGAA GTAAAACAAC AGAATCTGTC ACCAGAGATT
    GAAAAGCTAT GCTTTGCTCA CTCTGAAAAA ATATCAAGCT TTTCAAATTC TGTTACGCAA
    GTGGATTGCT TTATTAGAAA CAGTGAATAT AAAAAGCAAC CTGACTTCAG TAAGTTATAT
    TTCTTGCCTC GTCATAAGAA ACAATATGGA TTATACGTTA AATATACAAG CTGCCAGACT
    TCATCCCAGA GCTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020945912.1
    CDS398..4855
    Translation

    Target ORF information:

    RefSeq Version XM_021090253.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021090253.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCTGAGAT CACATGACTC CTTGTGTTCT TTGGATAATT TATTTTTTGA AAAAACAGAT 
    GAAGCTGAAA AATACCCAGA CAATGAAAAA TCATTAGATT GGTTTCTCCC ACCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAAGCA TTAGAGGAAG ACATGCAAAG TCATAAACTG
    TTAGGTCAGA AAAAGAGGTC AAAAATGTTA ACATCCAATT TAAAGATAAC TAATGAAGAC
    ACAAGTTATA CTTCACCAAC ACAAAAATTC CAGTGTGCTT TCCCTTCTAA TAAATACGAA
    GAAGGCGATC TAAATTTAGT AGGGGCAGGT AGCAATGACT TACATGTTGC TGGCAAGCTG
    ACTTATGGTT CTCCTCAGAA GTACAAAAGT CACATTGGCC CTGAGATAGC ACTTGAGAAA
    AATGTTCCAG ATGATGCGAA AGTAGTTAAT TTTGCATACG ATAAAGGAGA GAGCACATCG
    GCCTTCCGAA AAAGATTATT TAAAATATCT GATAGTATAC ATGGGAATGC TTGTTCTAAT
    GACATTGCAA ACTTGGACTC TGATATTGGC TCAGCGAAGA TTGCCCAAAT GGAAATGAGC
    GAAGGGAAAT CGTGGAAATG GAACAATAGT AATCAGAAAC CTCAATATTC AGAAATTAAC
    ATGTTTACAG CCAGTGATAC TTTTTCTGCT TCTAAAATCA GAGAAGGCAT ATTCAAAGCA
    CCATCTTTTT CAGTTGCATC CCAGCCTCAT GAAGTTCAAG GGATAATAGA AAATGGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATCCCG GCAAAATTTA GAAGTGTTTT CAAAGAATTC
    CCATATTTCA ACTATATCCA GTCTAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    GTTGTGGTTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAA TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGCAGTCA GCGTTTTGAT GACTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC GGGAGATACA GTCATGGATG ACCTGTTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCAACTGGT TCGACTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTT GTCAGCAGGA TGAAAACTGT GCAGTTTTTA
    TCTCATGCTG TGGAAAATCC CAGAAATATT ATTCCAATGA GATTTGTAGC TGTATCTGCA
    ACAATTCCAA ATGCTGAGGA TATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCCGCTATC
    TGTCTGAAAA TGGATGAGAA ACATAGACCA GTGAAGCTTC GGAAAGTGGT CCTTGGTTTT
    CCCTGCAGTA ATAACCAAAC TGAATTTAAG TTTGATTTAA CCCTCAACTA CAAAATTGCC
    AGTGTTGTAC AAACGTACTC CGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTACAAC AGGCTGCTTC CATTCTTGTG AAAGATGCCA AGTTTATTAT GACGGTGGAA
    CAGAAACAGA GGTTACATAA ATGTGCATAT TCCATAAGAG ATTCAAAATT GAGAGATCTC
    TTAATACACG GTGTTGCTTA TCATCATGCT GGCATGGAGC TGTCAGATAG AAAAGTAGTC
    GAAGGAGCTT TTACTGTTGG AGATCTACCA GTTCTTTTTA CTACTAGTAC CTTAGCTATG
    GGAGTAAATT TGCCTGCTCA TCTAGTAGTT ATCAAATCTA CAATGCATTA TGCTGGAGGG
    ATGTTTGAAG AGTACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCAAC TGCAGTTATT ATGACTCGGT TAAGTACAAA AGAGAAGTAT
    ATTCAGATGC TGGCTTGTAG TGACACTGTA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT GTTGCATACC ATCACAGATG TCAACATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTGTA TATCAGAGCC TTGAAGAATC CGTCTCACTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATTTG
    AATGATTTAT CATCTCTGGA CTTGATAAAG ATGGATGAAG ATATTAATTT CAAGCCAACT
    GAGGCAGGAA GATTGATGGC TTGGTACTAT GTTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATCAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG GATAAATGAA AAGAAAACAC TGAATACTTT GAACAAAGAT
    CCAAATCGGA TAACTATCAG ATTTCCAATG GAAGGAAAAA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCA TACAAGACTT TGCTTTGACA
    CAAGATACCT CAAAGATTTT CAGGAATGGC TCACGAATTA CGAGATGGTT ATCAGATTTT
    GTGGCTGTTC AAGAAAAGTT TGCTGTGCTT TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCTGCAT GTATCCAAAC AACTGGAAAA AATCGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AGCTTGAACT GATTTTAAAT AGACATCCCC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTGTCTGCC AAAATACGAA CTTGAAGTGG AACAGATTGC AAGATACAGT
    GATACCACGG CAGAAGTTTT AGTGACTGTT ATATTAAGAA ACTTCGAACC ACTGCAAACT
    AAAAGAACAG CACCAGATTC TCACTATGTT ACCTTAATCA TAGGCGATGC AGATAATCAG
    CTGATTTTTA AGCATAAAAT TATGGATTCT GTTTTGCTGA AATCTGGAAA TTGGGCTAAA
    AAGATCGATG TGAAGAGAGC TCTGAAATCT GAAGATCTTA GCATAAATCT CATTAGTTCT
    GAGTATGTTG GACTTGATAT TCAGCAGAAA TTTACAGTCT TTTACTTAGG ACCCAAGAGA
    TTTGGAAATC AAATAATTAT GCAAAAAAAA TCAGAAACAG AGATCTTCCA TTCTAAACGT
    TCAGATGGAT CTCCTGTAGC AGGTCCCATT AAAGGAATGA CTGCCTGCAG AAAGCCTGGG
    CACCGAGAGT GTAACCATCA CTGTAAAAAG AAACACACGT GTGGACATGA CTGCTGTAAA
    ACTGGAGTTG CCCAGAAGCC AGAAATGAAA GAGTCAACAA TTTCTTCTTA TTTATCTGAT
    TTAAGAAACA GGAATGCAGT TTCATCTCTT CCTCCAGTTA AACGTCTAAA GATGCAGATG
    AATAAATCTC AAAGTGTGGA CCTTAAAGAG TTTGGTTTTA CTCCACAACC TTCTCTTCCC
    AGTCTGTCAA GGTCAGAATG TTTAAACATA CCTAAATTGC CTGTAATGGA GCAGAGGGGT
    CAGCACGAGC TCTATGCAGA AGGTCAACAA CAAGAACCAC CGGGATATCA GGACAAAGGT
    TTTTCCAGAC AGACCTCTTC TACAACCTCA AATGAGAAAA CCCCTCAAAC AACACTGTTC
    AACAGAAATA TTAAGGCAAC TGGAAACCAG AATTTGACAG CTGTGGTTAA AAGTAATTCT
    TCCTATTCAG AAGTTTTGAA TGTGAACTTT GAATTGGGAA ATGAAGTTTG GGATGATTTT
    GATGATGAAA ACTTAATAGA AATTGCTAGC TTTTCAGCTG ATACTGAAAA GACAAAAGCA
    GGATTTGGAA ACACTTTGAG TTCAAGTGCC AGGGAAAATA AGCTACCCTT CCAAGAATCA
    AAAAGCAAAT TCCAAAGAAA AATGTCAAAC AGTTTTGTTT CATCACATGA GAAGCCAGAT
    ATTTATTTAC CAAATTCTGC TCTGTCCAGG ATCGGTGCAT CCTCCATGAC AAAAATACCT
    CAACCAGCTG GAAATGTCCC TTTCCAAGAA GTAAAACAAC AGAATCTGTC ACCAGAGATT
    GAAAAGCTAT GCTTTGCTCA CTCTGAAAAA ATATCAAGCT TTTCAAATTC TGTTACGCAA
    GTGGATTGCT TTATTAGAAA CAGTGAATAT AAAAAGCAAC CTGACTTCAG TAAGTTATAT
    TTCTTGCCTC GTCATAAGAA ACAATATGGA TTATACGTTA AATATACAAG CTGCCAGACT
    TCATCCCAGA GCTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe248243
    Clone ID Related Accession (Same CDS sequence) XM_013997383.2
    Accession Version XM_013997383.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4440bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product probable ATP-dependent DNA helicase HFM1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010446.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_013997383.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCTGAGAT CACATGACTC CTTGTGTTCT TTGGATAATT TATTTTTTGA AAAAACAGAT 
    GAAGCTGAAA AATACCCAGA CAATGAAAAA TCATTAGATT GGTTTCTCCC ACCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAAGCA TTAGAGGAAG ACATGCAAAG TCATAAACTG
    TTAGGTCAGA AAAAGAGGTC AAAAATGTTA ACATCCAATT TAAAGATAAC TAATGAAGAC
    ACAAGTTATA CTTCACCAAC ACAAAAATTC CAGTGTGCTT TCCCTTCTAA TAAATACGAA
    GAAGGCGATC TAAATTTAGT AGGGGCAGGT AGCAATGACT TACATGTTGC TGGCAAGCTG
    ACTTATGGTT CTCCTCAGAA GTACAAAAGT CACATTGGCC CTGAGATAGC ACTTGAGAAA
    AATGTTCCAG ATGATGCGAA AGTAGTTAAT TTTGCATACG ATAAAGGAGA GAGCACATCG
    GCCTTCCGAA AAAGATTATT TAAAATATCT GATAGTATAC ATGGGAATGC TTGTTCTAAT
    GACATTGCAA ACTTGGACTC TGATATTGGC TCAGCGAAGA TTGCCCAAAT GGAAATGAGC
    GAAGGGAAAT CGTGGAAATG GAACAATAGT AATCAGAAAC CTCAATATTC AGAAATTAAC
    ATGTTTACAG CCAGTGATAC TTTTTCTGCT TCTAAAATCA GAGAAGGCAT ATTCAAAGCA
    CCATCTTTTT CAGTTGCATC CCAGCCTCAT GAAGTTCAAG GGATAATAGA AAATGGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATCCCG GCAAAATTTA GAAGTGTTTT CAAAGAATTC
    CCATATTTCA ACTATATCCA GTCTAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    GTTGTGGTTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAA TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGCAGTCA GCGTTTTGAT GACTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC GGGAGATACA GTCATGGATG ACCTGTTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCAACTGGT TCGACTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTT GTCAGCAGGA TGAAAACTGT GCAGTTTTTA
    TCTCATGCTG TGGAAAATCC CAGAAATATT ATTCCAATGA GATTTGTAGC TGTATCTGCA
    ACAATTCCAA ATGCTGAGGA TATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCCGCTATC
    TGTCTGAAAA TGGATGAGAA ACATAGACCA GTGAAGCTTC GGAAAGTGGT CCTTGGTTTT
    CCCTGCAGTA ATAACCAAAC TGAATTTAAG TTTGATTTAA CCCTCAACTA CAAAATTGCC
    AGTGTTGTAC AAACGTACTC CGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTACAAC AGGCTGCTTC CATTCTTGTG AAAGATGCCA AGTTTATTAT GACGGTGGAA
    CAGAAACAGA GGTTACATAA ATGTGCATAT TCCATAAGAG ATTCAAAATT GAGAGATCTC
    TTAATACACG GTGTTGCTTA TCATCATGCT GGCATGGAGC TGTCAGATAG AAAAGTAGTC
    GAAGGAGCTT TTACTGTTGG AGATCTACCA GTTCTTTTTA CTACTAGTAC CTTAGCTATG
    GGAGTAAATT TGCCTGCTCA TCTAGTAGTT ATCAAATCTA CAATGCATTA TGCTGGAGGG
    ATGTTTGAAG AGTACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCAAC TGCAGTTATT ATGACTCGGT TAAGTACAAA AGAGAAGTAT
    ATTCAGATGC TGGCTTGTAG TGACACTGTA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT GTTGCATACC ATCACAGATG TCAACATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTGTA TATCAGAGCC TTGAAGAATC CGTCTCACTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATTTG
    AATGATTTAT CATCTCTGGA CTTGATAAAG ATGGATGAAG ATATTAATTT CAAGCCAACT
    GAGGCAGGAA GATTGATGGC TTGGTACTAT GTTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATCAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG GATAAATGAA AAGAAAACAC TGAATACTTT GAACAAAGAT
    CCAAATCGGA TAACTATCAG ATTTCCAATG GAAGGAAAAA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCA TACAAGACTT TGCTTTGACA
    CAAGATACCT CAAAGATTTT CAGGAATGGC TCACGAATTA CGAGATGGTT ATCAGATTTT
    GTGGCTGTTC AAGAAAAGTT TGCTGTGCTT TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCTGCAT GTATCCAAAC AACTGGAAAA AATCGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AGCTTGAACT GATTTTAAAT AGACATCCCC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTGTCTGCC AAAATACGAA CTTGAAGTGG AACAGATTGC AAGATACAGT
    GATACCACGG CAGAAGTTTT AGTGACTGTT ATATTAAGAA ACTTCGAACC ACTGCAAACT
    AAAAGAACAG CACCAGATTC TCACTATGTT ACCTTAATCA TAGGCGATGC AGATAATCAG
    CTGATTTTTA AGCATAAAAT TATGGATTCT GTTTTGCTGA AATCTGGAAA TTGGGCTAAA
    AAGATCGATG TGAAGAGAGC TCTGAAATCT GAAGATCTTA GCATAAATCT CATTAGTTCT
    GAGTATGTTG GACTTGATAT TCAGCAGAAA TTTACAGTCT TTTACTTAGG ACCCAAGAGA
    TTTGGAAATC AAATAATTAT GCAAAAAAAA TCAGAAACAG AGATCTTCCA TTCTAAACGT
    TCAGATGGAT CTCCTGTAGC AGGTCCCATT AAAGGAATGA CTGCCTGCAG AAAGCCTGGG
    CACCGAGAGT GTAACCATCA CTGTAAAAAG AAACACACGT GTGGACATGA CTGCTGTAAA
    ACTGGAGTTG CCCAGAAGCC AGAAATGAAA GAGTCAACAA TTTCTTCTTA TTTATCTGAT
    TTAAGAAACA GGAATGCAGT TTCATCTCTT CCTCCAGTTA AACGTCTAAA GATGCAGATG
    AATAAATCTC AAAGTGTGGA CCTTAAAGAG TTTGGTTTTA CTCCACAACC TTCTCTTCCC
    AGTCTGTCAA GGTCAGAATG TTTAAACATA CCTAAATTGC CTGTAATGGA GCAGAGGGGT
    CAGCACGAGC TCTATGCAGA AGGTCAACAA CAAGAACCAC CGGGATATCA GGACAAAGGT
    TTTTCCAGAC AGACCTCTTC TACAACCTCA AATGAGAAAA CCCCTCAAAC AACACTGTTC
    AACAGAAATA TTAAGGCAAC TGGAAACCAG AATTTGACAG CTGTGGTTAA AAGTAATTCT
    TCCTATTCAG AAGTTTTGAA TGTGAACTTT GAATTGGGAA ATGAAGTTTG GGATGATTTT
    GATGATGAAA ACTTAATAGA AATTGCTAGC TTTTCAGCTG ATACTGAAAA GACAAAAGCA
    GGATTTGGAA ACACTTTGAG TTCAAGTGCC AGGGAAAATA AGCTACCCTT CCAAGAATCA
    AAAAGCAAAT TCCAAAGAAA AATGTCAAAC AGTTTTGTTT CATCACATGA GAAGCCAGAT
    ATTTATTTAC CAAATTCTGC TCTGTCCAGG ATCGGTGCAT CCTCCATGAC AAAAATACCT
    CAACCAGCTG GAAATGTCCC TTTCCAAGAA GTAAAACAAC AGAATCTGTC ACCAGAGATT
    GAAAAGCTAT GCTTTGCTCA CTCTGAAAAA ATATCAAGCT TTTCAAATTC TGTTACGCAA
    GTGGATTGCT TTATTAGAAA CAGTGAATAT AAAAAGCAAC CTGACTTCAG TAGGTATCAT
    CTTGATGATG AAGCTGATGA AATGAAGTCT TTTCTGGGAA TATTTGATGG TATTTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013852837.2
    CDS103..4542
    Translation

    Target ORF information:

    RefSeq Version XM_013997383.2
    Organism Sus scrofa(pig)
    Definition Sus scrofa HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_013997383.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCTGAGAT CACATGACTC CTTGTGTTCT TTGGATAATT TATTTTTTGA AAAAACAGAT 
    GAAGCTGAAA AATACCCAGA CAATGAAAAA TCATTAGATT GGTTTCTCCC ACCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAAGCA TTAGAGGAAG ACATGCAAAG TCATAAACTG
    TTAGGTCAGA AAAAGAGGTC AAAAATGTTA ACATCCAATT TAAAGATAAC TAATGAAGAC
    ACAAGTTATA CTTCACCAAC ACAAAAATTC CAGTGTGCTT TCCCTTCTAA TAAATACGAA
    GAAGGCGATC TAAATTTAGT AGGGGCAGGT AGCAATGACT TACATGTTGC TGGCAAGCTG
    ACTTATGGTT CTCCTCAGAA GTACAAAAGT CACATTGGCC CTGAGATAGC ACTTGAGAAA
    AATGTTCCAG ATGATGCGAA AGTAGTTAAT TTTGCATACG ATAAAGGAGA GAGCACATCG
    GCCTTCCGAA AAAGATTATT TAAAATATCT GATAGTATAC ATGGGAATGC TTGTTCTAAT
    GACATTGCAA ACTTGGACTC TGATATTGGC TCAGCGAAGA TTGCCCAAAT GGAAATGAGC
    GAAGGGAAAT CGTGGAAATG GAACAATAGT AATCAGAAAC CTCAATATTC AGAAATTAAC
    ATGTTTACAG CCAGTGATAC TTTTTCTGCT TCTAAAATCA GAGAAGGCAT ATTCAAAGCA
    CCATCTTTTT CAGTTGCATC CCAGCCTCAT GAAGTTCAAG GGATAATAGA AAATGGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATCCCG GCAAAATTTA GAAGTGTTTT CAAAGAATTC
    CCATATTTCA ACTATATCCA GTCTAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    GTTGTGGTTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAA TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGCAGTCA GCGTTTTGAT GACTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC GGGAGATACA GTCATGGATG ACCTGTTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCAACTGGT TCGACTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTT GTCAGCAGGA TGAAAACTGT GCAGTTTTTA
    TCTCATGCTG TGGAAAATCC CAGAAATATT ATTCCAATGA GATTTGTAGC TGTATCTGCA
    ACAATTCCAA ATGCTGAGGA TATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCCGCTATC
    TGTCTGAAAA TGGATGAGAA ACATAGACCA GTGAAGCTTC GGAAAGTGGT CCTTGGTTTT
    CCCTGCAGTA ATAACCAAAC TGAATTTAAG TTTGATTTAA CCCTCAACTA CAAAATTGCC
    AGTGTTGTAC AAACGTACTC CGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTACAAC AGGCTGCTTC CATTCTTGTG AAAGATGCCA AGTTTATTAT GACGGTGGAA
    CAGAAACAGA GGTTACATAA ATGTGCATAT TCCATAAGAG ATTCAAAATT GAGAGATCTC
    TTAATACACG GTGTTGCTTA TCATCATGCT GGCATGGAGC TGTCAGATAG AAAAGTAGTC
    GAAGGAGCTT TTACTGTTGG AGATCTACCA GTTCTTTTTA CTACTAGTAC CTTAGCTATG
    GGAGTAAATT TGCCTGCTCA TCTAGTAGTT ATCAAATCTA CAATGCATTA TGCTGGAGGG
    ATGTTTGAAG AGTACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCAAC TGCAGTTATT ATGACTCGGT TAAGTACAAA AGAGAAGTAT
    ATTCAGATGC TGGCTTGTAG TGACACTGTA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT GTTGCATACC ATCACAGATG TCAACATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTGTA TATCAGAGCC TTGAAGAATC CGTCTCACTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATTTG
    AATGATTTAT CATCTCTGGA CTTGATAAAG ATGGATGAAG ATATTAATTT CAAGCCAACT
    GAGGCAGGAA GATTGATGGC TTGGTACTAT GTTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATCAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG GATAAATGAA AAGAAAACAC TGAATACTTT GAACAAAGAT
    CCAAATCGGA TAACTATCAG ATTTCCAATG GAAGGAAAAA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCA TACAAGACTT TGCTTTGACA
    CAAGATACCT CAAAGATTTT CAGGAATGGC TCACGAATTA CGAGATGGTT ATCAGATTTT
    GTGGCTGTTC AAGAAAAGTT TGCTGTGCTT TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCTGCAT GTATCCAAAC AACTGGAAAA AATCGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AGCTTGAACT GATTTTAAAT AGACATCCCC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTGTCTGCC AAAATACGAA CTTGAAGTGG AACAGATTGC AAGATACAGT
    GATACCACGG CAGAAGTTTT AGTGACTGTT ATATTAAGAA ACTTCGAACC ACTGCAAACT
    AAAAGAACAG CACCAGATTC TCACTATGTT ACCTTAATCA TAGGCGATGC AGATAATCAG
    CTGATTTTTA AGCATAAAAT TATGGATTCT GTTTTGCTGA AATCTGGAAA TTGGGCTAAA
    AAGATCGATG TGAAGAGAGC TCTGAAATCT GAAGATCTTA GCATAAATCT CATTAGTTCT
    GAGTATGTTG GACTTGATAT TCAGCAGAAA TTTACAGTCT TTTACTTAGG ACCCAAGAGA
    TTTGGAAATC AAATAATTAT GCAAAAAAAA TCAGAAACAG AGATCTTCCA TTCTAAACGT
    TCAGATGGAT CTCCTGTAGC AGGTCCCATT AAAGGAATGA CTGCCTGCAG AAAGCCTGGG
    CACCGAGAGT GTAACCATCA CTGTAAAAAG AAACACACGT GTGGACATGA CTGCTGTAAA
    ACTGGAGTTG CCCAGAAGCC AGAAATGAAA GAGTCAACAA TTTCTTCTTA TTTATCTGAT
    TTAAGAAACA GGAATGCAGT TTCATCTCTT CCTCCAGTTA AACGTCTAAA GATGCAGATG
    AATAAATCTC AAAGTGTGGA CCTTAAAGAG TTTGGTTTTA CTCCACAACC TTCTCTTCCC
    AGTCTGTCAA GGTCAGAATG TTTAAACATA CCTAAATTGC CTGTAATGGA GCAGAGGGGT
    CAGCACGAGC TCTATGCAGA AGGTCAACAA CAAGAACCAC CGGGATATCA GGACAAAGGT
    TTTTCCAGAC AGACCTCTTC TACAACCTCA AATGAGAAAA CCCCTCAAAC AACACTGTTC
    AACAGAAATA TTAAGGCAAC TGGAAACCAG AATTTGACAG CTGTGGTTAA AAGTAATTCT
    TCCTATTCAG AAGTTTTGAA TGTGAACTTT GAATTGGGAA ATGAAGTTTG GGATGATTTT
    GATGATGAAA ACTTAATAGA AATTGCTAGC TTTTCAGCTG ATACTGAAAA GACAAAAGCA
    GGATTTGGAA ACACTTTGAG TTCAAGTGCC AGGGAAAATA AGCTACCCTT CCAAGAATCA
    AAAAGCAAAT TCCAAAGAAA AATGTCAAAC AGTTTTGTTT CATCACATGA GAAGCCAGAT
    ATTTATTTAC CAAATTCTGC TCTGTCCAGG ATCGGTGCAT CCTCCATGAC AAAAATACCT
    CAACCAGCTG GAAATGTCCC TTTCCAAGAA GTAAAACAAC AGAATCTGTC ACCAGAGATT
    GAAAAGCTAT GCTTTGCTCA CTCTGAAAAA ATATCAAGCT TTTCAAATTC TGTTACGCAA
    GTGGATTGCT TTATTAGAAA CAGTGAATAT AAAAAGCAAC CTGACTTCAG TAGGTATCAT
    CTTGATGATG AAGCTGATGA AATGAAGTCT TTTCTGGGAA TATTTGATGG TATTTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe248244
    Clone ID Related Accession (Same CDS sequence) XM_013997386.2
    Accession Version XM_013997386.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4437bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product probable ATP-dependent DNA helicase HFM1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010446.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_013997386.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCTGAGAT CACATGACTC CTTGTGTTCT TTGGATAATT TATTTTTTGA AAAAACAGAT 
    GAAGCTGAAA AATACCCAGA CAATGAAAAA TCATTAGATT GGTTTCTCCC ACCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAAGCA TTAGAGGAAG ACATGCAAAG TCATAAACTG
    TTAGGTCAGA AAAAGAGGTC AAAAATGTTA ACATCCAATT TAAAGATAAC TAATGAAGAC
    ACAAGTTATA CTTCACCAAC ACAAAAATTC CAGTGTGCTT TCCCTTCTAA TAAATACGAA
    GAAGGCGATC TAAATTTAGT AGGGGCAGGT AGCAATGACT TACATGTTGC TGGCAAGCTG
    ACTTATGGTT CTCCTCAGAA GTACAAAAGT CACATTGGCC CTGAGATAGC ACTTGAGAAA
    AATGTTCCAG ATGATGCGAA AGTAGTTAAT TTTGCATACG ATAAAGGAGA GAGCACATCG
    GCCTTCCGAA AAAGATTATT TAAAATATCT GATAGTATAC ATGGGAATGC TTGTTCTAAT
    GACATTGCAA ACTTGGACTC TGATATTGGC TCAGCGAAGA TTGCCCAAAT GGAAATGAGC
    GAAGGGAAAT CGTGGAAATG GAACAATAGT AATCAGAAAC CTCAATATTC AGAAATTAAC
    ATGTTTACAG CCAGTGATAC TTTTTCTGCT TCTAAAATCA GAGAAGGCAT ATTCAAAGCA
    CCATCTTTTT CAGTTGCATC CCAGCCTCAT GAAGTTCAAG GGATAATAGA AAATGGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATCCCG GCAAAATTTA GAAGTGTTTT CAAAGAATTC
    CCATATTTCA ACTATATCCA GTCTAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    GTTGTGGTTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAA TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGCAGTCA GCGTTTTGAT GACTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC GGGAGATACA GTCATGGATG ACCTGTTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCAACTGGT TCGACTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTT GTCAGCAGGA TGAAAACTGT GCAGTTTTTA
    TCTCATGCTG TGGAAAATCC CAGAAATATT ATTCCAATGA GATTTGTAGC TGTATCTGCA
    ACAATTCCAA ATGCTGAGGA TATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCCGCTATC
    TGTCTGAAAA TGGATGAGAA ACATAGACCA GTGAAGCTTC GGAAAGTGGT CCTTGGTTTT
    CCCTGCAGTA ATAACCAAAC TGAATTTAAG TTTGATTTAA CCCTCAACTA CAAAATTGCC
    AGTGTTGTAC AAACGTACTC CGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTACAAC AGGCTGCTTC CATTCTTGTG AAAGATGCCA AGTTTATTAT GACGGTGGAA
    CAGAAACAGA GGTTACATAA ATGTGCATAT TCCATAAGAG ATTCAAAATT GAGAGATCTC
    TTAATACACG GTGTTGCTTA TCATCATGCT GGCATGGAGC TGTCAGATAG AAAAGTAGTC
    GAAGGAGCTT TTACTGTTGG AGATCTACCA GTTCTTTTTA CTACTAGTAC CTTAGCTATG
    GGAGTAAATT TGCCTGCTCA TCTAGTAGTT ATCAAATCTA CAATGCATTA TGCTGGAGGG
    ATGTTTGAAG AGTACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCAAC TGCAGTTATT ATGACTCGGT TAAGTACAAA AGAGAAGTAT
    ATTCAGATGC TGGCTTGTAG TGACACTGTA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT GTTGCATACC ATCACAGATG TCAACATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTGTA TATCAGAGCC TTGAAGAATC CGTCTCACTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATTTG
    AATGATTTAT CATCTCTGGA CTTGATAAAG ATGGATGAAG ATATTAATTT CAAGCCAACT
    GAGGCAGGAA GATTGATGGC TTGGTACTAT GTTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATCAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG GATAAATGAA AAGAAAACAC TGAATACTTT GAACAAAGAT
    CCAAATCGGA TAACTATCAG ATTTCCAATG GAAGGAAAAA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCA TACAAGACTT TGCTTTGACA
    CAAGATACCT CAAAGATTTT CAGGAATGGC TCACGAATTA CGAGATGGTT ATCAGATTTT
    GTGGCTGTTC AAGAAAAGTT TGCTGTGCTT TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCTGCAT GTATCCAAAC AACTGGAAAA AATCGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AGCTTGAACT GATTTTAAAT AGACATCCCC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTGTCTGCC AAAATACGAA CTTGAAGTGG AACAGATTGC AAGATACAGT
    GATACCACGG CAGAAGTTTT AGTGACTGTT ATATTAAGAA ACTTCGAACC ACTGCAAACT
    AAAAGAACAG CACCAGATTC TCACTATGTT ACCTTAATCA TAGGCGATGC AGATAATCAG
    CTGATTTTTA AGCATAAAAT TATGGATTCT GTTTTGCTGA AATCTGGAAA TTGGGCTAAA
    AAGATCGATG TGAAGAGAGC TCTGAAATCT GAAGATCTTA GCATAAATCT CATTAGTTCT
    GAGTATGTTG GACTTGATAT TCAGCAGAAA TTTACAGTCT TTTACTTAGG ACCCAAGAGA
    TTTGGAAATC AAATAATTAT GCAAAAAAAA TCAGAAACAG AGATCTTCCA TTCTAAACGT
    TCAGATGGAT CTCCTGTAGC AGGTCCCATT AAAGGAATGA CTGCCTGCAG AAAGCCTGGG
    CACCGAGAGT GTAACCATCA CTGTAAAAAG AAACACACGT GTGGACATGA CTGCTGTAAA
    ACTGGAGTTG CCCAGAAGCC AGAAATGAAA GAGTCAACAA TTTCTTCTTA TTTATCTGAT
    TTAAGAAACA GGAATGCAGT TTCATCTCTT CCTCCAGTTA AACGTCTAAA GATGCAGATG
    AATAAATCTC AAAGTGTGGA CCTTAAAGAG TTTGGTTTTA CTCCACAACC TTCTCTTCCC
    AGTCTGTCAA GGTCAGAATG TTTAAACATA CCTAAATTGC CTGTAATGGA GCAGAGGGGT
    CAGCACGAGC TCTATGCAGA AGGTCAACAA CAAGAACCAC CGGGATATCA GGACAAAGGT
    TTTTCCAGAC AGACCTCTTC TACAACCTCA AATGAGAAAA CCCCTCAAAC AACACTGTTC
    AACAGAAATA TTAAGGCAAC TGGAAACCAG AATTTGACAG CTGTGGTTAA AAGTAATTCT
    TCCTATTCAG AAGTTTTGAA TGTGAACTTT GAATTGGGAA ATGAAGTTTG GGATGATTTT
    GATGATGAAA ACTTAATAGA AATTGCTAGC TTTTCAGCTG ATACTGAAAA GACAAAAGCA
    GGATTTGGAA ACACTTTGAG TTCAAGTGCC AGGGAAAATA AGCTACCCTT CCAAGAATCA
    AAAAGCAAAT TCCAAAGAAA AATGTCAAAC AGTTTTGTTT CATCACATGA GAAGCCAGAT
    ATTTATTTAC CAAATTCTGC TCTGTCCAGG ATCGGTGCAT CCTCCATGAC AAAAATACCT
    CAACCAGCTG GAAATGTCCC TTTCCAAGAA GTAAAACAAC AGAATCTGTC ACCAGAGATT
    GAAAAGCTAT GCTTTGCTCA CTCTGAAAAA ATATCAAGCT TTTCAAATTC TGTTACGCAA
    GTGGATTGCT TTATTAGAAA CAGTGAATAT AAAAAGCAAC CTGACTTCAG GTATCATCTT
    GATGATGAAG CTGATGAAAT GAAGTCTTTT CTGGGAATAT TTGATGGTAT TTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013852840.2
    CDS103..4539
    Translation

    Target ORF information:

    RefSeq Version XM_013997386.2
    Organism Sus scrofa(pig)
    Definition Sus scrofa HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_013997386.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCTGAGAT CACATGACTC CTTGTGTTCT TTGGATAATT TATTTTTTGA AAAAACAGAT 
    GAAGCTGAAA AATACCCAGA CAATGAAAAA TCATTAGATT GGTTTCTCCC ACCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAAGCA TTAGAGGAAG ACATGCAAAG TCATAAACTG
    TTAGGTCAGA AAAAGAGGTC AAAAATGTTA ACATCCAATT TAAAGATAAC TAATGAAGAC
    ACAAGTTATA CTTCACCAAC ACAAAAATTC CAGTGTGCTT TCCCTTCTAA TAAATACGAA
    GAAGGCGATC TAAATTTAGT AGGGGCAGGT AGCAATGACT TACATGTTGC TGGCAAGCTG
    ACTTATGGTT CTCCTCAGAA GTACAAAAGT CACATTGGCC CTGAGATAGC ACTTGAGAAA
    AATGTTCCAG ATGATGCGAA AGTAGTTAAT TTTGCATACG ATAAAGGAGA GAGCACATCG
    GCCTTCCGAA AAAGATTATT TAAAATATCT GATAGTATAC ATGGGAATGC TTGTTCTAAT
    GACATTGCAA ACTTGGACTC TGATATTGGC TCAGCGAAGA TTGCCCAAAT GGAAATGAGC
    GAAGGGAAAT CGTGGAAATG GAACAATAGT AATCAGAAAC CTCAATATTC AGAAATTAAC
    ATGTTTACAG CCAGTGATAC TTTTTCTGCT TCTAAAATCA GAGAAGGCAT ATTCAAAGCA
    CCATCTTTTT CAGTTGCATC CCAGCCTCAT GAAGTTCAAG GGATAATAGA AAATGGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATCCCG GCAAAATTTA GAAGTGTTTT CAAAGAATTC
    CCATATTTCA ACTATATCCA GTCTAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    GTTGTGGTTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAA TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGCAGTCA GCGTTTTGAT GACTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC GGGAGATACA GTCATGGATG ACCTGTTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCAACTGGT TCGACTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTT GTCAGCAGGA TGAAAACTGT GCAGTTTTTA
    TCTCATGCTG TGGAAAATCC CAGAAATATT ATTCCAATGA GATTTGTAGC TGTATCTGCA
    ACAATTCCAA ATGCTGAGGA TATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCCGCTATC
    TGTCTGAAAA TGGATGAGAA ACATAGACCA GTGAAGCTTC GGAAAGTGGT CCTTGGTTTT
    CCCTGCAGTA ATAACCAAAC TGAATTTAAG TTTGATTTAA CCCTCAACTA CAAAATTGCC
    AGTGTTGTAC AAACGTACTC CGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTACAAC AGGCTGCTTC CATTCTTGTG AAAGATGCCA AGTTTATTAT GACGGTGGAA
    CAGAAACAGA GGTTACATAA ATGTGCATAT TCCATAAGAG ATTCAAAATT GAGAGATCTC
    TTAATACACG GTGTTGCTTA TCATCATGCT GGCATGGAGC TGTCAGATAG AAAAGTAGTC
    GAAGGAGCTT TTACTGTTGG AGATCTACCA GTTCTTTTTA CTACTAGTAC CTTAGCTATG
    GGAGTAAATT TGCCTGCTCA TCTAGTAGTT ATCAAATCTA CAATGCATTA TGCTGGAGGG
    ATGTTTGAAG AGTACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCAAC TGCAGTTATT ATGACTCGGT TAAGTACAAA AGAGAAGTAT
    ATTCAGATGC TGGCTTGTAG TGACACTGTA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT GTTGCATACC ATCACAGATG TCAACATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTGTA TATCAGAGCC TTGAAGAATC CGTCTCACTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATTTG
    AATGATTTAT CATCTCTGGA CTTGATAAAG ATGGATGAAG ATATTAATTT CAAGCCAACT
    GAGGCAGGAA GATTGATGGC TTGGTACTAT GTTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATCAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG GATAAATGAA AAGAAAACAC TGAATACTTT GAACAAAGAT
    CCAAATCGGA TAACTATCAG ATTTCCAATG GAAGGAAAAA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCA TACAAGACTT TGCTTTGACA
    CAAGATACCT CAAAGATTTT CAGGAATGGC TCACGAATTA CGAGATGGTT ATCAGATTTT
    GTGGCTGTTC AAGAAAAGTT TGCTGTGCTT TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCTGCAT GTATCCAAAC AACTGGAAAA AATCGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AGCTTGAACT GATTTTAAAT AGACATCCCC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTGTCTGCC AAAATACGAA CTTGAAGTGG AACAGATTGC AAGATACAGT
    GATACCACGG CAGAAGTTTT AGTGACTGTT ATATTAAGAA ACTTCGAACC ACTGCAAACT
    AAAAGAACAG CACCAGATTC TCACTATGTT ACCTTAATCA TAGGCGATGC AGATAATCAG
    CTGATTTTTA AGCATAAAAT TATGGATTCT GTTTTGCTGA AATCTGGAAA TTGGGCTAAA
    AAGATCGATG TGAAGAGAGC TCTGAAATCT GAAGATCTTA GCATAAATCT CATTAGTTCT
    GAGTATGTTG GACTTGATAT TCAGCAGAAA TTTACAGTCT TTTACTTAGG ACCCAAGAGA
    TTTGGAAATC AAATAATTAT GCAAAAAAAA TCAGAAACAG AGATCTTCCA TTCTAAACGT
    TCAGATGGAT CTCCTGTAGC AGGTCCCATT AAAGGAATGA CTGCCTGCAG AAAGCCTGGG
    CACCGAGAGT GTAACCATCA CTGTAAAAAG AAACACACGT GTGGACATGA CTGCTGTAAA
    ACTGGAGTTG CCCAGAAGCC AGAAATGAAA GAGTCAACAA TTTCTTCTTA TTTATCTGAT
    TTAAGAAACA GGAATGCAGT TTCATCTCTT CCTCCAGTTA AACGTCTAAA GATGCAGATG
    AATAAATCTC AAAGTGTGGA CCTTAAAGAG TTTGGTTTTA CTCCACAACC TTCTCTTCCC
    AGTCTGTCAA GGTCAGAATG TTTAAACATA CCTAAATTGC CTGTAATGGA GCAGAGGGGT
    CAGCACGAGC TCTATGCAGA AGGTCAACAA CAAGAACCAC CGGGATATCA GGACAAAGGT
    TTTTCCAGAC AGACCTCTTC TACAACCTCA AATGAGAAAA CCCCTCAAAC AACACTGTTC
    AACAGAAATA TTAAGGCAAC TGGAAACCAG AATTTGACAG CTGTGGTTAA AAGTAATTCT
    TCCTATTCAG AAGTTTTGAA TGTGAACTTT GAATTGGGAA ATGAAGTTTG GGATGATTTT
    GATGATGAAA ACTTAATAGA AATTGCTAGC TTTTCAGCTG ATACTGAAAA GACAAAAGCA
    GGATTTGGAA ACACTTTGAG TTCAAGTGCC AGGGAAAATA AGCTACCCTT CCAAGAATCA
    AAAAGCAAAT TCCAAAGAAA AATGTCAAAC AGTTTTGTTT CATCACATGA GAAGCCAGAT
    ATTTATTTAC CAAATTCTGC TCTGTCCAGG ATCGGTGCAT CCTCCATGAC AAAAATACCT
    CAACCAGCTG GAAATGTCCC TTTCCAAGAA GTAAAACAAC AGAATCTGTC ACCAGAGATT
    GAAAAGCTAT GCTTTGCTCA CTCTGAAAAA ATATCAAGCT TTTCAAATTC TGTTACGCAA
    GTGGATTGCT TTATTAGAAA CAGTGAATAT AAAAAGCAAC CTGACTTCAG GTATCATCTT
    GATGATGAAG CTGATGAAAT GAAGTCTTTT CTGGGAATAT TTGATGGTAT TTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe248245
    Clone ID Related Accession (Same CDS sequence) XM_021090257.1 , XM_021090256.1
    Accession Version XM_021090257.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4332bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product probable ATP-dependent DNA helicase HFM1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010446.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGACTCCTT GTGTTCTTTG GATAATTTAT TTTTTGAAAA AACAGATGAA GCTGAAAAGT 
    CAGAAAAAGA GGTCAAAAAT GTTAACATCC AATTTAAAGA TAACTAATGA AGACACAAGT
    TATACTTCAC CAACACAAAA ATTCCAGTGT GCTTTCCCTT CTAATAAATA CGAAGAAGGC
    GATCTAAATT TAGTAGGGGC AGGTAGCAAT GACTTACATG TTGCTGGCAA GCTGACTTAT
    GGTTCTCCTC AGAAGTACAA AAGTCACATT GGCCCTGAGA TAGCACTTGA GAAAAATGTT
    CCAGATGATG CGAAAGTAGT TAATTTTGCA TACGATAAAG GAGAGAGCAC ATCGGCCTTC
    CGAAAAAGAT TATTTAAAAT ATCTGATAGT ATACATGGGA ATGCTTGTTC TAATGACATT
    GCAAACTTGG ACTCTGATAT TGGCTCAGCG AAGATTGCCC AAATGGAAAT GAGCGAAGGG
    AAATCGTGGA AATGGAACAA TAGTAATCAG AAACCTCAAT ATTCAGAAAT TAACATGTTT
    ACAGCCAGTG ATACTTTTTC TGCTTCTAAA ATCAGAGAAG GCATATTCAA AGCACCATCT
    TTTTCAGTTG CATCCCAGCC TCATGAAGTT CAAGGGATAA TAGAAAATGG TTTAGATTCC
    TTGAAGGCTG TCACAGAAAT CCCGGCAAAA TTTAGAAGTG TTTTCAAAGA ATTCCCATAT
    TTCAACTATA TCCAGTCTAA AGCCTTTGAT GATCTTCTTT ATACAGATAG GAATGTTGTG
    GTTTGTGCTC CAACTGGTTC TGGGAAAACT GTAATGTTTG AACTAGCTAT AACAAGATTG
    TTAATGGAAG TACCATTGCC ATGGTCAAAC ATTAAAATTG TTTACATGGC ACCAATAAAA
    GCCCTGTGCA GTCAGCGTTT TGATGACTGG AAAGAAAAAT TTGGACCAAT AGGATTGAAT
    TGTAAAGAAC TCACGGGAGA TACAGTCATG GATGACCTGT TTGAGATTCA GCATGCCCAT
    ATTATTATGA CAACTCCAGA AAAATGGGAT AGCATGACTA GAAAATGGAG AGACAACTCT
    TTGGTCCAAC TGGTTCGACT GTTTCTCATT GATGAGGTAC ATGTTGTAAA AGATGAAAAT
    CGTGGACCAA CTCTTGAAGT TGTTGTCAGC AGGATGAAAA CTGTGCAGTT TTTATCTCAT
    GCTGTGGAAA ATCCCAGAAA TATTATTCCA ATGAGATTTG TAGCTGTATC TGCAACAATT
    CCAAATGCTG AGGATATTGC AGAATGGCTT TCAGATGGTG AAAGATCCGC TATCTGTCTG
    AAAATGGATG AGAAACATAG ACCAGTGAAG CTTCGGAAAG TGGTCCTTGG TTTTCCCTGC
    AGTAATAACC AAACTGAATT TAAGTTTGAT TTAACCCTCA ACTACAAAAT TGCCAGTGTT
    GTACAAACGT ACTCCGATCA GAAACCCACA CTTGTGTTTT GTGCAACAAG GAAAGGTGTA
    CAACAGGCTG CTTCCATTCT TGTGAAAGAT GCCAAGTTTA TTATGACGGT GGAACAGAAA
    CAGAGGTTAC ATAAATGTGC ATATTCCATA AGAGATTCAA AATTGAGAGA TCTCTTAATA
    CACGGTGTTG CTTATCATCA TGCTGGCATG GAGCTGTCAG ATAGAAAAGT AGTCGAAGGA
    GCTTTTACTG TTGGAGATCT ACCAGTTCTT TTTACTACTA GTACCTTAGC TATGGGAGTA
    AATTTGCCTG CTCATCTAGT AGTTATCAAA TCTACAATGC ATTATGCTGG AGGGATGTTT
    GAAGAGTACA GTGAAACAGA TATTCTACAG ATGATTGGTA GAGCTGGTCG ACCTCAATTT
    GACACTACAG CAACTGCAGT TATTATGACT CGGTTAAGTA CAAAAGAGAA GTATATTCAG
    ATGCTGGCTT GTAGTGACAC TGTAGAAAGC AGTTTGCACA GACATCTTAT TGAGCATTTA
    AATGCAGAGA TAGTGTTGCA TACCATCACA GATGTCAACA TTGCTTTGGA ATGGATACGA
    TCAACCTTGC TGTATATCAG AGCCTTGAAG AATCCGTCTC ACTATGGTTT TGCATCTGGA
    TTGAACAAAG ATGGAATTGA ATCAAAATTA CAAGAATTGT GTTTGAAGAA TTTGAATGAT
    TTATCATCTC TGGACTTGAT AAAGATGGAT GAAGATATTA ATTTCAAGCC AACTGAGGCA
    GGAAGATTGA TGGCTTGGTA CTATGTTACA TTTGAGACAG TGAAGAAATT TTGTACAATC
    AGTGGAAAAG AAACTTTATC AGATCTGGTC ACAATGATAG CCAGCTGCAA GGAATTTCTA
    GATGTACAAT TAAGGATAAA TGAAAAGAAA ACACTGAATA CTTTGAACAA AGATCCAAAT
    CGGATAACTA TCAGATTTCC AATGGAAGGA AAAATTAAAA CAAGAGAAAT GAAAGTAAAT
    TGTCTTATTC AGGCTCAGCT AGGATGCATT CCCATACAAG ACTTTGCTTT GACACAAGAT
    ACCTCAAAGA TTTTCAGGAA TGGCTCACGA ATTACGAGAT GGTTATCAGA TTTTGTGGCT
    GTTCAAGAAA AGTTTGCTGT GCTTTTGAAC AGTTTGATTT TAGCTAAATG TTTTAGATGT
    AAACTTTGGG AAAACTCTCT GCATGTATCC AAACAACTGG AAAAAATCGG TATAACATTA
    TCAAATGCTA TGGTAAATGC AGGTTTGACT TCCTTTAAAA AAATAGAAGA AACAGATGCA
    AGGGAGCTTG AACTGATTTT AAATAGACAT CCCCCTTTTG GAACTCAGAT AAAAGAAACA
    GTGATGTGTC TGCCAAAATA CGAACTTGAA GTGGAACAGA TTGCAAGATA CAGTGATACC
    ACGGCAGAAG TTTTAGTGAC TGTTATATTA AGAAACTTCG AACCACTGCA AACTAAAAGA
    ACAGCACCAG ATTCTCACTA TGTTACCTTA ATCATAGGCG ATGCAGATAA TCAGCTGATT
    TTTAAGCATA AAATTATGGA TTCTGTTTTG CTGAAATCTG GAAATTGGGC TAAAAAGATC
    GATGTGAAGA GAGCTCTGAA ATCTGAAGAT CTTAGCATAA ATCTCATTAG TTCTGAGTAT
    GTTGGACTTG ATATTCAGCA GAAATTTACA GTCTTTTACT TAGGACCCAA GAGATTTGGA
    AATCAAATAA TTATGCAAAA AAAATCAGAA ACAGAGATCT TCCATTCTAA ACGTTCAGAT
    GGATCTCCTG TAGCAGGTCC CATTAAAGGA ATGACTGCCT GCAGAAAGCC TGGGCACCGA
    GAGTGTAACC ATCACTGTAA AAAGAAACAC ACGTGTGGAC ATGACTGCTG TAAAACTGGA
    GTTGCCCAGA AGCCAGAAAT GAAAGAGTCA ACAATTTCTT CTTATTTATC TGATTTAAGA
    AACAGGAATG CAGTTTCATC TCTTCCTCCA GTTAAACGTC TAAAGATGCA GATGAATAAA
    TCTCAAAGTG TGGACCTTAA AGAGTTTGGT TTTACTCCAC AACCTTCTCT TCCCAGTCTG
    TCAAGGTCAG AATGTTTAAA CATACCTAAA TTGCCTGTAA TGGAGCAGAG GGGTCAGCAC
    GAGCTCTATG CAGAAGGTCA ACAACAAGAA CCACCGGGAT ATCAGGACAA AGGTTTTTCC
    AGACAGACCT CTTCTACAAC CTCAAATGAG AAAACCCCTC AAACAACACT GTTCAACAGA
    AATATTAAGG CAACTGGAAA CCAGAATTTG ACAGCTGTGG TTAAAAGTAA TTCTTCCTAT
    TCAGAAGTTT TGAATGTGAA CTTTGAATTG GGAAATGAAG TTTGGGATGA TTTTGATGAT
    GAAAACTTAA TAGAAATTGC TAGCTTTTCA GCTGATACTG AAAAGACAAA AGCAGGATTT
    GGAAACACTT TGAGTTCAAG TGCCAGGGAA AATAAGCTAC CCTTCCAAGA ATCAAAAAGC
    AAATTCCAAA GAAAAATGTC AAACAGTTTT GTTTCATCAC ATGAGAAGCC AGATATTTAT
    TTACCAAATT CTGCTCTGTC CAGGATCGGT GCATCCTCCA TGACAAAAAT ACCTCAACCA
    GCTGGAAATG TCCCTTTCCA AGAAGTAAAA CAACAGAATC TGTCACCAGA GATTGAAAAG
    CTATGCTTTG CTCACTCTGA AAAAATATCA AGCTTTTCAA ATTCTGTTAC GCAAGTGGAT
    TGCTTTATTA GAAACAGTGA ATATAAAAAG CAACCTGACT TCAGTAAGTT ATATTTCTTG
    CCTCGTCATA AGAAACAATA TGGATTATAC GTTAAATATA CAAGCTGCCA GACTTCATCC
    CAGAGCTCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020945916.1
    CDS410..4741
    Translation

    Target ORF information:

    RefSeq Version XM_021090257.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021090257.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGACTCCTT GTGTTCTTTG GATAATTTAT TTTTTGAAAA AACAGATGAA GCTGAAAAGT 
    CAGAAAAAGA GGTCAAAAAT GTTAACATCC AATTTAAAGA TAACTAATGA AGACACAAGT
    TATACTTCAC CAACACAAAA ATTCCAGTGT GCTTTCCCTT CTAATAAATA CGAAGAAGGC
    GATCTAAATT TAGTAGGGGC AGGTAGCAAT GACTTACATG TTGCTGGCAA GCTGACTTAT
    GGTTCTCCTC AGAAGTACAA AAGTCACATT GGCCCTGAGA TAGCACTTGA GAAAAATGTT
    CCAGATGATG CGAAAGTAGT TAATTTTGCA TACGATAAAG GAGAGAGCAC ATCGGCCTTC
    CGAAAAAGAT TATTTAAAAT ATCTGATAGT ATACATGGGA ATGCTTGTTC TAATGACATT
    GCAAACTTGG ACTCTGATAT TGGCTCAGCG AAGATTGCCC AAATGGAAAT GAGCGAAGGG
    AAATCGTGGA AATGGAACAA TAGTAATCAG AAACCTCAAT ATTCAGAAAT TAACATGTTT
    ACAGCCAGTG ATACTTTTTC TGCTTCTAAA ATCAGAGAAG GCATATTCAA AGCACCATCT
    TTTTCAGTTG CATCCCAGCC TCATGAAGTT CAAGGGATAA TAGAAAATGG TTTAGATTCC
    TTGAAGGCTG TCACAGAAAT CCCGGCAAAA TTTAGAAGTG TTTTCAAAGA ATTCCCATAT
    TTCAACTATA TCCAGTCTAA AGCCTTTGAT GATCTTCTTT ATACAGATAG GAATGTTGTG
    GTTTGTGCTC CAACTGGTTC TGGGAAAACT GTAATGTTTG AACTAGCTAT AACAAGATTG
    TTAATGGAAG TACCATTGCC ATGGTCAAAC ATTAAAATTG TTTACATGGC ACCAATAAAA
    GCCCTGTGCA GTCAGCGTTT TGATGACTGG AAAGAAAAAT TTGGACCAAT AGGATTGAAT
    TGTAAAGAAC TCACGGGAGA TACAGTCATG GATGACCTGT TTGAGATTCA GCATGCCCAT
    ATTATTATGA CAACTCCAGA AAAATGGGAT AGCATGACTA GAAAATGGAG AGACAACTCT
    TTGGTCCAAC TGGTTCGACT GTTTCTCATT GATGAGGTAC ATGTTGTAAA AGATGAAAAT
    CGTGGACCAA CTCTTGAAGT TGTTGTCAGC AGGATGAAAA CTGTGCAGTT TTTATCTCAT
    GCTGTGGAAA ATCCCAGAAA TATTATTCCA ATGAGATTTG TAGCTGTATC TGCAACAATT
    CCAAATGCTG AGGATATTGC AGAATGGCTT TCAGATGGTG AAAGATCCGC TATCTGTCTG
    AAAATGGATG AGAAACATAG ACCAGTGAAG CTTCGGAAAG TGGTCCTTGG TTTTCCCTGC
    AGTAATAACC AAACTGAATT TAAGTTTGAT TTAACCCTCA ACTACAAAAT TGCCAGTGTT
    GTACAAACGT ACTCCGATCA GAAACCCACA CTTGTGTTTT GTGCAACAAG GAAAGGTGTA
    CAACAGGCTG CTTCCATTCT TGTGAAAGAT GCCAAGTTTA TTATGACGGT GGAACAGAAA
    CAGAGGTTAC ATAAATGTGC ATATTCCATA AGAGATTCAA AATTGAGAGA TCTCTTAATA
    CACGGTGTTG CTTATCATCA TGCTGGCATG GAGCTGTCAG ATAGAAAAGT AGTCGAAGGA
    GCTTTTACTG TTGGAGATCT ACCAGTTCTT TTTACTACTA GTACCTTAGC TATGGGAGTA
    AATTTGCCTG CTCATCTAGT AGTTATCAAA TCTACAATGC ATTATGCTGG AGGGATGTTT
    GAAGAGTACA GTGAAACAGA TATTCTACAG ATGATTGGTA GAGCTGGTCG ACCTCAATTT
    GACACTACAG CAACTGCAGT TATTATGACT CGGTTAAGTA CAAAAGAGAA GTATATTCAG
    ATGCTGGCTT GTAGTGACAC TGTAGAAAGC AGTTTGCACA GACATCTTAT TGAGCATTTA
    AATGCAGAGA TAGTGTTGCA TACCATCACA GATGTCAACA TTGCTTTGGA ATGGATACGA
    TCAACCTTGC TGTATATCAG AGCCTTGAAG AATCCGTCTC ACTATGGTTT TGCATCTGGA
    TTGAACAAAG ATGGAATTGA ATCAAAATTA CAAGAATTGT GTTTGAAGAA TTTGAATGAT
    TTATCATCTC TGGACTTGAT AAAGATGGAT GAAGATATTA ATTTCAAGCC AACTGAGGCA
    GGAAGATTGA TGGCTTGGTA CTATGTTACA TTTGAGACAG TGAAGAAATT TTGTACAATC
    AGTGGAAAAG AAACTTTATC AGATCTGGTC ACAATGATAG CCAGCTGCAA GGAATTTCTA
    GATGTACAAT TAAGGATAAA TGAAAAGAAA ACACTGAATA CTTTGAACAA AGATCCAAAT
    CGGATAACTA TCAGATTTCC AATGGAAGGA AAAATTAAAA CAAGAGAAAT GAAAGTAAAT
    TGTCTTATTC AGGCTCAGCT AGGATGCATT CCCATACAAG ACTTTGCTTT GACACAAGAT
    ACCTCAAAGA TTTTCAGGAA TGGCTCACGA ATTACGAGAT GGTTATCAGA TTTTGTGGCT
    GTTCAAGAAA AGTTTGCTGT GCTTTTGAAC AGTTTGATTT TAGCTAAATG TTTTAGATGT
    AAACTTTGGG AAAACTCTCT GCATGTATCC AAACAACTGG AAAAAATCGG TATAACATTA
    TCAAATGCTA TGGTAAATGC AGGTTTGACT TCCTTTAAAA AAATAGAAGA AACAGATGCA
    AGGGAGCTTG AACTGATTTT AAATAGACAT CCCCCTTTTG GAACTCAGAT AAAAGAAACA
    GTGATGTGTC TGCCAAAATA CGAACTTGAA GTGGAACAGA TTGCAAGATA CAGTGATACC
    ACGGCAGAAG TTTTAGTGAC TGTTATATTA AGAAACTTCG AACCACTGCA AACTAAAAGA
    ACAGCACCAG ATTCTCACTA TGTTACCTTA ATCATAGGCG ATGCAGATAA TCAGCTGATT
    TTTAAGCATA AAATTATGGA TTCTGTTTTG CTGAAATCTG GAAATTGGGC TAAAAAGATC
    GATGTGAAGA GAGCTCTGAA ATCTGAAGAT CTTAGCATAA ATCTCATTAG TTCTGAGTAT
    GTTGGACTTG ATATTCAGCA GAAATTTACA GTCTTTTACT TAGGACCCAA GAGATTTGGA
    AATCAAATAA TTATGCAAAA AAAATCAGAA ACAGAGATCT TCCATTCTAA ACGTTCAGAT
    GGATCTCCTG TAGCAGGTCC CATTAAAGGA ATGACTGCCT GCAGAAAGCC TGGGCACCGA
    GAGTGTAACC ATCACTGTAA AAAGAAACAC ACGTGTGGAC ATGACTGCTG TAAAACTGGA
    GTTGCCCAGA AGCCAGAAAT GAAAGAGTCA ACAATTTCTT CTTATTTATC TGATTTAAGA
    AACAGGAATG CAGTTTCATC TCTTCCTCCA GTTAAACGTC TAAAGATGCA GATGAATAAA
    TCTCAAAGTG TGGACCTTAA AGAGTTTGGT TTTACTCCAC AACCTTCTCT TCCCAGTCTG
    TCAAGGTCAG AATGTTTAAA CATACCTAAA TTGCCTGTAA TGGAGCAGAG GGGTCAGCAC
    GAGCTCTATG CAGAAGGTCA ACAACAAGAA CCACCGGGAT ATCAGGACAA AGGTTTTTCC
    AGACAGACCT CTTCTACAAC CTCAAATGAG AAAACCCCTC AAACAACACT GTTCAACAGA
    AATATTAAGG CAACTGGAAA CCAGAATTTG ACAGCTGTGG TTAAAAGTAA TTCTTCCTAT
    TCAGAAGTTT TGAATGTGAA CTTTGAATTG GGAAATGAAG TTTGGGATGA TTTTGATGAT
    GAAAACTTAA TAGAAATTGC TAGCTTTTCA GCTGATACTG AAAAGACAAA AGCAGGATTT
    GGAAACACTT TGAGTTCAAG TGCCAGGGAA AATAAGCTAC CCTTCCAAGA ATCAAAAAGC
    AAATTCCAAA GAAAAATGTC AAACAGTTTT GTTTCATCAC ATGAGAAGCC AGATATTTAT
    TTACCAAATT CTGCTCTGTC CAGGATCGGT GCATCCTCCA TGACAAAAAT ACCTCAACCA
    GCTGGAAATG TCCCTTTCCA AGAAGTAAAA CAACAGAATC TGTCACCAGA GATTGAAAAG
    CTATGCTTTG CTCACTCTGA AAAAATATCA AGCTTTTCAA ATTCTGTTAC GCAAGTGGAT
    TGCTTTATTA GAAACAGTGA ATATAAAAAG CAACCTGACT TCAGTAAGTT ATATTTCTTG
    CCTCGTCATA AGAAACAATA TGGATTATAC GTTAAATATA CAAGCTGCCA GACTTCATCC
    CAGAGCTCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe248245
    Clone ID Related Accession (Same CDS sequence) XM_021090257.1 , XM_021090256.1
    Accession Version XM_021090256.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4332bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product probable ATP-dependent DNA helicase HFM1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010446.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGACTCCTT GTGTTCTTTG GATAATTTAT TTTTTGAAAA AACAGATGAA GCTGAAAAGT 
    CAGAAAAAGA GGTCAAAAAT GTTAACATCC AATTTAAAGA TAACTAATGA AGACACAAGT
    TATACTTCAC CAACACAAAA ATTCCAGTGT GCTTTCCCTT CTAATAAATA CGAAGAAGGC
    GATCTAAATT TAGTAGGGGC AGGTAGCAAT GACTTACATG TTGCTGGCAA GCTGACTTAT
    GGTTCTCCTC AGAAGTACAA AAGTCACATT GGCCCTGAGA TAGCACTTGA GAAAAATGTT
    CCAGATGATG CGAAAGTAGT TAATTTTGCA TACGATAAAG GAGAGAGCAC ATCGGCCTTC
    CGAAAAAGAT TATTTAAAAT ATCTGATAGT ATACATGGGA ATGCTTGTTC TAATGACATT
    GCAAACTTGG ACTCTGATAT TGGCTCAGCG AAGATTGCCC AAATGGAAAT GAGCGAAGGG
    AAATCGTGGA AATGGAACAA TAGTAATCAG AAACCTCAAT ATTCAGAAAT TAACATGTTT
    ACAGCCAGTG ATACTTTTTC TGCTTCTAAA ATCAGAGAAG GCATATTCAA AGCACCATCT
    TTTTCAGTTG CATCCCAGCC TCATGAAGTT CAAGGGATAA TAGAAAATGG TTTAGATTCC
    TTGAAGGCTG TCACAGAAAT CCCGGCAAAA TTTAGAAGTG TTTTCAAAGA ATTCCCATAT
    TTCAACTATA TCCAGTCTAA AGCCTTTGAT GATCTTCTTT ATACAGATAG GAATGTTGTG
    GTTTGTGCTC CAACTGGTTC TGGGAAAACT GTAATGTTTG AACTAGCTAT AACAAGATTG
    TTAATGGAAG TACCATTGCC ATGGTCAAAC ATTAAAATTG TTTACATGGC ACCAATAAAA
    GCCCTGTGCA GTCAGCGTTT TGATGACTGG AAAGAAAAAT TTGGACCAAT AGGATTGAAT
    TGTAAAGAAC TCACGGGAGA TACAGTCATG GATGACCTGT TTGAGATTCA GCATGCCCAT
    ATTATTATGA CAACTCCAGA AAAATGGGAT AGCATGACTA GAAAATGGAG AGACAACTCT
    TTGGTCCAAC TGGTTCGACT GTTTCTCATT GATGAGGTAC ATGTTGTAAA AGATGAAAAT
    CGTGGACCAA CTCTTGAAGT TGTTGTCAGC AGGATGAAAA CTGTGCAGTT TTTATCTCAT
    GCTGTGGAAA ATCCCAGAAA TATTATTCCA ATGAGATTTG TAGCTGTATC TGCAACAATT
    CCAAATGCTG AGGATATTGC AGAATGGCTT TCAGATGGTG AAAGATCCGC TATCTGTCTG
    AAAATGGATG AGAAACATAG ACCAGTGAAG CTTCGGAAAG TGGTCCTTGG TTTTCCCTGC
    AGTAATAACC AAACTGAATT TAAGTTTGAT TTAACCCTCA ACTACAAAAT TGCCAGTGTT
    GTACAAACGT ACTCCGATCA GAAACCCACA CTTGTGTTTT GTGCAACAAG GAAAGGTGTA
    CAACAGGCTG CTTCCATTCT TGTGAAAGAT GCCAAGTTTA TTATGACGGT GGAACAGAAA
    CAGAGGTTAC ATAAATGTGC ATATTCCATA AGAGATTCAA AATTGAGAGA TCTCTTAATA
    CACGGTGTTG CTTATCATCA TGCTGGCATG GAGCTGTCAG ATAGAAAAGT AGTCGAAGGA
    GCTTTTACTG TTGGAGATCT ACCAGTTCTT TTTACTACTA GTACCTTAGC TATGGGAGTA
    AATTTGCCTG CTCATCTAGT AGTTATCAAA TCTACAATGC ATTATGCTGG AGGGATGTTT
    GAAGAGTACA GTGAAACAGA TATTCTACAG ATGATTGGTA GAGCTGGTCG ACCTCAATTT
    GACACTACAG CAACTGCAGT TATTATGACT CGGTTAAGTA CAAAAGAGAA GTATATTCAG
    ATGCTGGCTT GTAGTGACAC TGTAGAAAGC AGTTTGCACA GACATCTTAT TGAGCATTTA
    AATGCAGAGA TAGTGTTGCA TACCATCACA GATGTCAACA TTGCTTTGGA ATGGATACGA
    TCAACCTTGC TGTATATCAG AGCCTTGAAG AATCCGTCTC ACTATGGTTT TGCATCTGGA
    TTGAACAAAG ATGGAATTGA ATCAAAATTA CAAGAATTGT GTTTGAAGAA TTTGAATGAT
    TTATCATCTC TGGACTTGAT AAAGATGGAT GAAGATATTA ATTTCAAGCC AACTGAGGCA
    GGAAGATTGA TGGCTTGGTA CTATGTTACA TTTGAGACAG TGAAGAAATT TTGTACAATC
    AGTGGAAAAG AAACTTTATC AGATCTGGTC ACAATGATAG CCAGCTGCAA GGAATTTCTA
    GATGTACAAT TAAGGATAAA TGAAAAGAAA ACACTGAATA CTTTGAACAA AGATCCAAAT
    CGGATAACTA TCAGATTTCC AATGGAAGGA AAAATTAAAA CAAGAGAAAT GAAAGTAAAT
    TGTCTTATTC AGGCTCAGCT AGGATGCATT CCCATACAAG ACTTTGCTTT GACACAAGAT
    ACCTCAAAGA TTTTCAGGAA TGGCTCACGA ATTACGAGAT GGTTATCAGA TTTTGTGGCT
    GTTCAAGAAA AGTTTGCTGT GCTTTTGAAC AGTTTGATTT TAGCTAAATG TTTTAGATGT
    AAACTTTGGG AAAACTCTCT GCATGTATCC AAACAACTGG AAAAAATCGG TATAACATTA
    TCAAATGCTA TGGTAAATGC AGGTTTGACT TCCTTTAAAA AAATAGAAGA AACAGATGCA
    AGGGAGCTTG AACTGATTTT AAATAGACAT CCCCCTTTTG GAACTCAGAT AAAAGAAACA
    GTGATGTGTC TGCCAAAATA CGAACTTGAA GTGGAACAGA TTGCAAGATA CAGTGATACC
    ACGGCAGAAG TTTTAGTGAC TGTTATATTA AGAAACTTCG AACCACTGCA AACTAAAAGA
    ACAGCACCAG ATTCTCACTA TGTTACCTTA ATCATAGGCG ATGCAGATAA TCAGCTGATT
    TTTAAGCATA AAATTATGGA TTCTGTTTTG CTGAAATCTG GAAATTGGGC TAAAAAGATC
    GATGTGAAGA GAGCTCTGAA ATCTGAAGAT CTTAGCATAA ATCTCATTAG TTCTGAGTAT
    GTTGGACTTG ATATTCAGCA GAAATTTACA GTCTTTTACT TAGGACCCAA GAGATTTGGA
    AATCAAATAA TTATGCAAAA AAAATCAGAA ACAGAGATCT TCCATTCTAA ACGTTCAGAT
    GGATCTCCTG TAGCAGGTCC CATTAAAGGA ATGACTGCCT GCAGAAAGCC TGGGCACCGA
    GAGTGTAACC ATCACTGTAA AAAGAAACAC ACGTGTGGAC ATGACTGCTG TAAAACTGGA
    GTTGCCCAGA AGCCAGAAAT GAAAGAGTCA ACAATTTCTT CTTATTTATC TGATTTAAGA
    AACAGGAATG CAGTTTCATC TCTTCCTCCA GTTAAACGTC TAAAGATGCA GATGAATAAA
    TCTCAAAGTG TGGACCTTAA AGAGTTTGGT TTTACTCCAC AACCTTCTCT TCCCAGTCTG
    TCAAGGTCAG AATGTTTAAA CATACCTAAA TTGCCTGTAA TGGAGCAGAG GGGTCAGCAC
    GAGCTCTATG CAGAAGGTCA ACAACAAGAA CCACCGGGAT ATCAGGACAA AGGTTTTTCC
    AGACAGACCT CTTCTACAAC CTCAAATGAG AAAACCCCTC AAACAACACT GTTCAACAGA
    AATATTAAGG CAACTGGAAA CCAGAATTTG ACAGCTGTGG TTAAAAGTAA TTCTTCCTAT
    TCAGAAGTTT TGAATGTGAA CTTTGAATTG GGAAATGAAG TTTGGGATGA TTTTGATGAT
    GAAAACTTAA TAGAAATTGC TAGCTTTTCA GCTGATACTG AAAAGACAAA AGCAGGATTT
    GGAAACACTT TGAGTTCAAG TGCCAGGGAA AATAAGCTAC CCTTCCAAGA ATCAAAAAGC
    AAATTCCAAA GAAAAATGTC AAACAGTTTT GTTTCATCAC ATGAGAAGCC AGATATTTAT
    TTACCAAATT CTGCTCTGTC CAGGATCGGT GCATCCTCCA TGACAAAAAT ACCTCAACCA
    GCTGGAAATG TCCCTTTCCA AGAAGTAAAA CAACAGAATC TGTCACCAGA GATTGAAAAG
    CTATGCTTTG CTCACTCTGA AAAAATATCA AGCTTTTCAA ATTCTGTTAC GCAAGTGGAT
    TGCTTTATTA GAAACAGTGA ATATAAAAAG CAACCTGACT TCAGTAAGTT ATATTTCTTG
    CCTCGTCATA AGAAACAATA TGGATTATAC GTTAAATATA CAAGCTGCCA GACTTCATCC
    CAGAGCTCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020945915.1
    CDS84..4415
    Translation

    Target ORF information:

    RefSeq Version XM_021090256.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021090256.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGACTCCTT GTGTTCTTTG GATAATTTAT TTTTTGAAAA AACAGATGAA GCTGAAAAGT 
    CAGAAAAAGA GGTCAAAAAT GTTAACATCC AATTTAAAGA TAACTAATGA AGACACAAGT
    TATACTTCAC CAACACAAAA ATTCCAGTGT GCTTTCCCTT CTAATAAATA CGAAGAAGGC
    GATCTAAATT TAGTAGGGGC AGGTAGCAAT GACTTACATG TTGCTGGCAA GCTGACTTAT
    GGTTCTCCTC AGAAGTACAA AAGTCACATT GGCCCTGAGA TAGCACTTGA GAAAAATGTT
    CCAGATGATG CGAAAGTAGT TAATTTTGCA TACGATAAAG GAGAGAGCAC ATCGGCCTTC
    CGAAAAAGAT TATTTAAAAT ATCTGATAGT ATACATGGGA ATGCTTGTTC TAATGACATT
    GCAAACTTGG ACTCTGATAT TGGCTCAGCG AAGATTGCCC AAATGGAAAT GAGCGAAGGG
    AAATCGTGGA AATGGAACAA TAGTAATCAG AAACCTCAAT ATTCAGAAAT TAACATGTTT
    ACAGCCAGTG ATACTTTTTC TGCTTCTAAA ATCAGAGAAG GCATATTCAA AGCACCATCT
    TTTTCAGTTG CATCCCAGCC TCATGAAGTT CAAGGGATAA TAGAAAATGG TTTAGATTCC
    TTGAAGGCTG TCACAGAAAT CCCGGCAAAA TTTAGAAGTG TTTTCAAAGA ATTCCCATAT
    TTCAACTATA TCCAGTCTAA AGCCTTTGAT GATCTTCTTT ATACAGATAG GAATGTTGTG
    GTTTGTGCTC CAACTGGTTC TGGGAAAACT GTAATGTTTG AACTAGCTAT AACAAGATTG
    TTAATGGAAG TACCATTGCC ATGGTCAAAC ATTAAAATTG TTTACATGGC ACCAATAAAA
    GCCCTGTGCA GTCAGCGTTT TGATGACTGG AAAGAAAAAT TTGGACCAAT AGGATTGAAT
    TGTAAAGAAC TCACGGGAGA TACAGTCATG GATGACCTGT TTGAGATTCA GCATGCCCAT
    ATTATTATGA CAACTCCAGA AAAATGGGAT AGCATGACTA GAAAATGGAG AGACAACTCT
    TTGGTCCAAC TGGTTCGACT GTTTCTCATT GATGAGGTAC ATGTTGTAAA AGATGAAAAT
    CGTGGACCAA CTCTTGAAGT TGTTGTCAGC AGGATGAAAA CTGTGCAGTT TTTATCTCAT
    GCTGTGGAAA ATCCCAGAAA TATTATTCCA ATGAGATTTG TAGCTGTATC TGCAACAATT
    CCAAATGCTG AGGATATTGC AGAATGGCTT TCAGATGGTG AAAGATCCGC TATCTGTCTG
    AAAATGGATG AGAAACATAG ACCAGTGAAG CTTCGGAAAG TGGTCCTTGG TTTTCCCTGC
    AGTAATAACC AAACTGAATT TAAGTTTGAT TTAACCCTCA ACTACAAAAT TGCCAGTGTT
    GTACAAACGT ACTCCGATCA GAAACCCACA CTTGTGTTTT GTGCAACAAG GAAAGGTGTA
    CAACAGGCTG CTTCCATTCT TGTGAAAGAT GCCAAGTTTA TTATGACGGT GGAACAGAAA
    CAGAGGTTAC ATAAATGTGC ATATTCCATA AGAGATTCAA AATTGAGAGA TCTCTTAATA
    CACGGTGTTG CTTATCATCA TGCTGGCATG GAGCTGTCAG ATAGAAAAGT AGTCGAAGGA
    GCTTTTACTG TTGGAGATCT ACCAGTTCTT TTTACTACTA GTACCTTAGC TATGGGAGTA
    AATTTGCCTG CTCATCTAGT AGTTATCAAA TCTACAATGC ATTATGCTGG AGGGATGTTT
    GAAGAGTACA GTGAAACAGA TATTCTACAG ATGATTGGTA GAGCTGGTCG ACCTCAATTT
    GACACTACAG CAACTGCAGT TATTATGACT CGGTTAAGTA CAAAAGAGAA GTATATTCAG
    ATGCTGGCTT GTAGTGACAC TGTAGAAAGC AGTTTGCACA GACATCTTAT TGAGCATTTA
    AATGCAGAGA TAGTGTTGCA TACCATCACA GATGTCAACA TTGCTTTGGA ATGGATACGA
    TCAACCTTGC TGTATATCAG AGCCTTGAAG AATCCGTCTC ACTATGGTTT TGCATCTGGA
    TTGAACAAAG ATGGAATTGA ATCAAAATTA CAAGAATTGT GTTTGAAGAA TTTGAATGAT
    TTATCATCTC TGGACTTGAT AAAGATGGAT GAAGATATTA ATTTCAAGCC AACTGAGGCA
    GGAAGATTGA TGGCTTGGTA CTATGTTACA TTTGAGACAG TGAAGAAATT TTGTACAATC
    AGTGGAAAAG AAACTTTATC AGATCTGGTC ACAATGATAG CCAGCTGCAA GGAATTTCTA
    GATGTACAAT TAAGGATAAA TGAAAAGAAA ACACTGAATA CTTTGAACAA AGATCCAAAT
    CGGATAACTA TCAGATTTCC AATGGAAGGA AAAATTAAAA CAAGAGAAAT GAAAGTAAAT
    TGTCTTATTC AGGCTCAGCT AGGATGCATT CCCATACAAG ACTTTGCTTT GACACAAGAT
    ACCTCAAAGA TTTTCAGGAA TGGCTCACGA ATTACGAGAT GGTTATCAGA TTTTGTGGCT
    GTTCAAGAAA AGTTTGCTGT GCTTTTGAAC AGTTTGATTT TAGCTAAATG TTTTAGATGT
    AAACTTTGGG AAAACTCTCT GCATGTATCC AAACAACTGG AAAAAATCGG TATAACATTA
    TCAAATGCTA TGGTAAATGC AGGTTTGACT TCCTTTAAAA AAATAGAAGA AACAGATGCA
    AGGGAGCTTG AACTGATTTT AAATAGACAT CCCCCTTTTG GAACTCAGAT AAAAGAAACA
    GTGATGTGTC TGCCAAAATA CGAACTTGAA GTGGAACAGA TTGCAAGATA CAGTGATACC
    ACGGCAGAAG TTTTAGTGAC TGTTATATTA AGAAACTTCG AACCACTGCA AACTAAAAGA
    ACAGCACCAG ATTCTCACTA TGTTACCTTA ATCATAGGCG ATGCAGATAA TCAGCTGATT
    TTTAAGCATA AAATTATGGA TTCTGTTTTG CTGAAATCTG GAAATTGGGC TAAAAAGATC
    GATGTGAAGA GAGCTCTGAA ATCTGAAGAT CTTAGCATAA ATCTCATTAG TTCTGAGTAT
    GTTGGACTTG ATATTCAGCA GAAATTTACA GTCTTTTACT TAGGACCCAA GAGATTTGGA
    AATCAAATAA TTATGCAAAA AAAATCAGAA ACAGAGATCT TCCATTCTAA ACGTTCAGAT
    GGATCTCCTG TAGCAGGTCC CATTAAAGGA ATGACTGCCT GCAGAAAGCC TGGGCACCGA
    GAGTGTAACC ATCACTGTAA AAAGAAACAC ACGTGTGGAC ATGACTGCTG TAAAACTGGA
    GTTGCCCAGA AGCCAGAAAT GAAAGAGTCA ACAATTTCTT CTTATTTATC TGATTTAAGA
    AACAGGAATG CAGTTTCATC TCTTCCTCCA GTTAAACGTC TAAAGATGCA GATGAATAAA
    TCTCAAAGTG TGGACCTTAA AGAGTTTGGT TTTACTCCAC AACCTTCTCT TCCCAGTCTG
    TCAAGGTCAG AATGTTTAAA CATACCTAAA TTGCCTGTAA TGGAGCAGAG GGGTCAGCAC
    GAGCTCTATG CAGAAGGTCA ACAACAAGAA CCACCGGGAT ATCAGGACAA AGGTTTTTCC
    AGACAGACCT CTTCTACAAC CTCAAATGAG AAAACCCCTC AAACAACACT GTTCAACAGA
    AATATTAAGG CAACTGGAAA CCAGAATTTG ACAGCTGTGG TTAAAAGTAA TTCTTCCTAT
    TCAGAAGTTT TGAATGTGAA CTTTGAATTG GGAAATGAAG TTTGGGATGA TTTTGATGAT
    GAAAACTTAA TAGAAATTGC TAGCTTTTCA GCTGATACTG AAAAGACAAA AGCAGGATTT
    GGAAACACTT TGAGTTCAAG TGCCAGGGAA AATAAGCTAC CCTTCCAAGA ATCAAAAAGC
    AAATTCCAAA GAAAAATGTC AAACAGTTTT GTTTCATCAC ATGAGAAGCC AGATATTTAT
    TTACCAAATT CTGCTCTGTC CAGGATCGGT GCATCCTCCA TGACAAAAAT ACCTCAACCA
    GCTGGAAATG TCCCTTTCCA AGAAGTAAAA CAACAGAATC TGTCACCAGA GATTGAAAAG
    CTATGCTTTG CTCACTCTGA AAAAATATCA AGCTTTTCAA ATTCTGTTAC GCAAGTGGAT
    TGCTTTATTA GAAACAGTGA ATATAAAAAG CAACCTGACT TCAGTAAGTT ATATTTCTTG
    CCTCGTCATA AGAAACAATA TGGATTATAC GTTAAATATA CAAGCTGCCA GACTTCATCC
    CAGAGCTCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe248246
    Clone ID Related Accession (Same CDS sequence) XM_013997387.2
    Accession Version XM_013997387.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4314bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product probable ATP-dependent DNA helicase HFM1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010446.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_013997387.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGACTCCTT GTGTTCTTTG GATAATTTAT TTTTTGAAAA AACAGATGAA GCTGAAAAGT 
    CAGAAAAAGA GGTCAAAAAT GTTAACATCC AATTTAAAGA TAACTAATGA AGACACAAGT
    TATACTTCAC CAACACAAAA ATTCCAGTGT GCTTTCCCTT CTAATAAATA CGAAGAAGGC
    GATCTAAATT TAGTAGGGGC AGGTAGCAAT GACTTACATG TTGCTGGCAA GCTGACTTAT
    GGTTCTCCTC AGAAGTACAA AAGTCACATT GGCCCTGAGA TAGCACTTGA GAAAAATGTT
    CCAGATGATG CGAAAGTAGT TAATTTTGCA TACGATAAAG GAGAGAGCAC ATCGGCCTTC
    CGAAAAAGAT TATTTAAAAT ATCTGATAGT ATACATGGGA ATGCTTGTTC TAATGACATT
    GCAAACTTGG ACTCTGATAT TGGCTCAGCG AAGATTGCCC AAATGGAAAT GAGCGAAGGG
    AAATCGTGGA AATGGAACAA TAGTAATCAG AAACCTCAAT ATTCAGAAAT TAACATGTTT
    ACAGCCAGTG ATACTTTTTC TGCTTCTAAA ATCAGAGAAG GCATATTCAA AGCACCATCT
    TTTTCAGTTG CATCCCAGCC TCATGAAGTT CAAGGGATAA TAGAAAATGG TTTAGATTCC
    TTGAAGGCTG TCACAGAAAT CCCGGCAAAA TTTAGAAGTG TTTTCAAAGA ATTCCCATAT
    TTCAACTATA TCCAGTCTAA AGCCTTTGAT GATCTTCTTT ATACAGATAG GAATGTTGTG
    GTTTGTGCTC CAACTGGTTC TGGGAAAACT GTAATGTTTG AACTAGCTAT AACAAGATTG
    TTAATGGAAG TACCATTGCC ATGGTCAAAC ATTAAAATTG TTTACATGGC ACCAATAAAA
    GCCCTGTGCA GTCAGCGTTT TGATGACTGG AAAGAAAAAT TTGGACCAAT AGGATTGAAT
    TGTAAAGAAC TCACGGGAGA TACAGTCATG GATGACCTGT TTGAGATTCA GCATGCCCAT
    ATTATTATGA CAACTCCAGA AAAATGGGAT AGCATGACTA GAAAATGGAG AGACAACTCT
    TTGGTCCAAC TGGTTCGACT GTTTCTCATT GATGAGGTAC ATGTTGTAAA AGATGAAAAT
    CGTGGACCAA CTCTTGAAGT TGTTGTCAGC AGGATGAAAA CTGTGCAGTT TTTATCTCAT
    GCTGTGGAAA ATCCCAGAAA TATTATTCCA ATGAGATTTG TAGCTGTATC TGCAACAATT
    CCAAATGCTG AGGATATTGC AGAATGGCTT TCAGATGGTG AAAGATCCGC TATCTGTCTG
    AAAATGGATG AGAAACATAG ACCAGTGAAG CTTCGGAAAG TGGTCCTTGG TTTTCCCTGC
    AGTAATAACC AAACTGAATT TAAGTTTGAT TTAACCCTCA ACTACAAAAT TGCCAGTGTT
    GTACAAACGT ACTCCGATCA GAAACCCACA CTTGTGTTTT GTGCAACAAG GAAAGGTGTA
    CAACAGGCTG CTTCCATTCT TGTGAAAGAT GCCAAGTTTA TTATGACGGT GGAACAGAAA
    CAGAGGTTAC ATAAATGTGC ATATTCCATA AGAGATTCAA AATTGAGAGA TCTCTTAATA
    CACGGTGTTG CTTATCATCA TGCTGGCATG GAGCTGTCAG ATAGAAAAGT AGTCGAAGGA
    GCTTTTACTG TTGGAGATCT ACCAGTTCTT TTTACTACTA GTACCTTAGC TATGGGAGTA
    AATTTGCCTG CTCATCTAGT AGTTATCAAA TCTACAATGC ATTATGCTGG AGGGATGTTT
    GAAGAGTACA GTGAAACAGA TATTCTACAG ATGATTGGTA GAGCTGGTCG ACCTCAATTT
    GACACTACAG CAACTGCAGT TATTATGACT CGGTTAAGTA CAAAAGAGAA GTATATTCAG
    ATGCTGGCTT GTAGTGACAC TGTAGAAAGC AGTTTGCACA GACATCTTAT TGAGCATTTA
    AATGCAGAGA TAGTGTTGCA TACCATCACA GATGTCAACA TTGCTTTGGA ATGGATACGA
    TCAACCTTGC TGTATATCAG AGCCTTGAAG AATCCGTCTC ACTATGGTTT TGCATCTGGA
    TTGAACAAAG ATGGAATTGA ATCAAAATTA CAAGAATTGT GTTTGAAGAA TTTGAATGAT
    TTATCATCTC TGGACTTGAT AAAGATGGAT GAAGATATTA ATTTCAAGCC AACTGAGGCA
    GGAAGATTGA TGGCTTGGTA CTATGTTACA TTTGAGACAG TGAAGAAATT TTGTACAATC
    AGTGGAAAAG AAACTTTATC AGATCTGGTC ACAATGATAG CCAGCTGCAA GGAATTTCTA
    GATGTACAAT TAAGGATAAA TGAAAAGAAA ACACTGAATA CTTTGAACAA AGATCCAAAT
    CGGATAACTA TCAGATTTCC AATGGAAGGA AAAATTAAAA CAAGAGAAAT GAAAGTAAAT
    TGTCTTATTC AGGCTCAGCT AGGATGCATT CCCATACAAG ACTTTGCTTT GACACAAGAT
    ACCTCAAAGA TTTTCAGGAA TGGCTCACGA ATTACGAGAT GGTTATCAGA TTTTGTGGCT
    GTTCAAGAAA AGTTTGCTGT GCTTTTGAAC AGTTTGATTT TAGCTAAATG TTTTAGATGT
    AAACTTTGGG AAAACTCTCT GCATGTATCC AAACAACTGG AAAAAATCGG TATAACATTA
    TCAAATGCTA TGGTAAATGC AGGTTTGACT TCCTTTAAAA AAATAGAAGA AACAGATGCA
    AGGGAGCTTG AACTGATTTT AAATAGACAT CCCCCTTTTG GAACTCAGAT AAAAGAAACA
    GTGATGTGTC TGCCAAAATA CGAACTTGAA GTGGAACAGA TTGCAAGATA CAGTGATACC
    ACGGCAGAAG TTTTAGTGAC TGTTATATTA AGAAACTTCG AACCACTGCA AACTAAAAGA
    ACAGCACCAG ATTCTCACTA TGTTACCTTA ATCATAGGCG ATGCAGATAA TCAGCTGATT
    TTTAAGCATA AAATTATGGA TTCTGTTTTG CTGAAATCTG GAAATTGGGC TAAAAAGATC
    GATGTGAAGA GAGCTCTGAA ATCTGAAGAT CTTAGCATAA ATCTCATTAG TTCTGAGTAT
    GTTGGACTTG ATATTCAGCA GAAATTTACA GTCTTTTACT TAGGACCCAA GAGATTTGGA
    AATCAAATAA TTATGCAAAA AAAATCAGAA ACAGAGATCT TCCATTCTAA ACGTTCAGAT
    GGATCTCCTG TAGCAGGTCC CATTAAAGGA ATGACTGCCT GCAGAAAGCC TGGGCACCGA
    GAGTGTAACC ATCACTGTAA AAAGAAACAC ACGTGTGGAC ATGACTGCTG TAAAACTGGA
    GTTGCCCAGA AGCCAGAAAT GAAAGAGTCA ACAATTTCTT CTTATTTATC TGATTTAAGA
    AACAGGAATG CAGTTTCATC TCTTCCTCCA GTTAAACGTC TAAAGATGCA GATGAATAAA
    TCTCAAAGTG TGGACCTTAA AGAGTTTGGT TTTACTCCAC AACCTTCTCT TCCCAGTCTG
    TCAAGGTCAG AATGTTTAAA CATACCTAAA TTGCCTGTAA TGGAGCAGAG GGGTCAGCAC
    GAGCTCTATG CAGAAGGTCA ACAACAAGAA CCACCGGGAT ATCAGGACAA AGGTTTTTCC
    AGACAGACCT CTTCTACAAC CTCAAATGAG AAAACCCCTC AAACAACACT GTTCAACAGA
    AATATTAAGG CAACTGGAAA CCAGAATTTG ACAGCTGTGG TTAAAAGTAA TTCTTCCTAT
    TCAGAAGTTT TGAATGTGAA CTTTGAATTG GGAAATGAAG TTTGGGATGA TTTTGATGAT
    GAAAACTTAA TAGAAATTGC TAGCTTTTCA GCTGATACTG AAAAGACAAA AGCAGGATTT
    GGAAACACTT TGAGTTCAAG TGCCAGGGAA AATAAGCTAC CCTTCCAAGA ATCAAAAAGC
    AAATTCCAAA GAAAAATGTC AAACAGTTTT GTTTCATCAC ATGAGAAGCC AGATATTTAT
    TTACCAAATT CTGCTCTGTC CAGGATCGGT GCATCCTCCA TGACAAAAAT ACCTCAACCA
    GCTGGAAATG TCCCTTTCCA AGAAGTAAAA CAACAGAATC TGTCACCAGA GATTGAAAAG
    CTATGCTTTG CTCACTCTGA AAAAATATCA AGCTTTTCAA ATTCTGTTAC GCAAGTGGAT
    TGCTTTATTA GAAACAGTGA ATATAAAAAG CAACCTGACT TCAGTAGGTA TCATCTTGAT
    GATGAAGCTG ATGAAATGAA GTCTTTTCTG GGAATATTTG ATGGTATTTT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013852841.2
    CDS84..4397
    Translation

    Target ORF information:

    RefSeq Version XM_013997387.2
    Organism Sus scrofa(pig)
    Definition Sus scrofa HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_013997387.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGACTCCTT GTGTTCTTTG GATAATTTAT TTTTTGAAAA AACAGATGAA GCTGAAAAGT 
    CAGAAAAAGA GGTCAAAAAT GTTAACATCC AATTTAAAGA TAACTAATGA AGACACAAGT
    TATACTTCAC CAACACAAAA ATTCCAGTGT GCTTTCCCTT CTAATAAATA CGAAGAAGGC
    GATCTAAATT TAGTAGGGGC AGGTAGCAAT GACTTACATG TTGCTGGCAA GCTGACTTAT
    GGTTCTCCTC AGAAGTACAA AAGTCACATT GGCCCTGAGA TAGCACTTGA GAAAAATGTT
    CCAGATGATG CGAAAGTAGT TAATTTTGCA TACGATAAAG GAGAGAGCAC ATCGGCCTTC
    CGAAAAAGAT TATTTAAAAT ATCTGATAGT ATACATGGGA ATGCTTGTTC TAATGACATT
    GCAAACTTGG ACTCTGATAT TGGCTCAGCG AAGATTGCCC AAATGGAAAT GAGCGAAGGG
    AAATCGTGGA AATGGAACAA TAGTAATCAG AAACCTCAAT ATTCAGAAAT TAACATGTTT
    ACAGCCAGTG ATACTTTTTC TGCTTCTAAA ATCAGAGAAG GCATATTCAA AGCACCATCT
    TTTTCAGTTG CATCCCAGCC TCATGAAGTT CAAGGGATAA TAGAAAATGG TTTAGATTCC
    TTGAAGGCTG TCACAGAAAT CCCGGCAAAA TTTAGAAGTG TTTTCAAAGA ATTCCCATAT
    TTCAACTATA TCCAGTCTAA AGCCTTTGAT GATCTTCTTT ATACAGATAG GAATGTTGTG
    GTTTGTGCTC CAACTGGTTC TGGGAAAACT GTAATGTTTG AACTAGCTAT AACAAGATTG
    TTAATGGAAG TACCATTGCC ATGGTCAAAC ATTAAAATTG TTTACATGGC ACCAATAAAA
    GCCCTGTGCA GTCAGCGTTT TGATGACTGG AAAGAAAAAT TTGGACCAAT AGGATTGAAT
    TGTAAAGAAC TCACGGGAGA TACAGTCATG GATGACCTGT TTGAGATTCA GCATGCCCAT
    ATTATTATGA CAACTCCAGA AAAATGGGAT AGCATGACTA GAAAATGGAG AGACAACTCT
    TTGGTCCAAC TGGTTCGACT GTTTCTCATT GATGAGGTAC ATGTTGTAAA AGATGAAAAT
    CGTGGACCAA CTCTTGAAGT TGTTGTCAGC AGGATGAAAA CTGTGCAGTT TTTATCTCAT
    GCTGTGGAAA ATCCCAGAAA TATTATTCCA ATGAGATTTG TAGCTGTATC TGCAACAATT
    CCAAATGCTG AGGATATTGC AGAATGGCTT TCAGATGGTG AAAGATCCGC TATCTGTCTG
    AAAATGGATG AGAAACATAG ACCAGTGAAG CTTCGGAAAG TGGTCCTTGG TTTTCCCTGC
    AGTAATAACC AAACTGAATT TAAGTTTGAT TTAACCCTCA ACTACAAAAT TGCCAGTGTT
    GTACAAACGT ACTCCGATCA GAAACCCACA CTTGTGTTTT GTGCAACAAG GAAAGGTGTA
    CAACAGGCTG CTTCCATTCT TGTGAAAGAT GCCAAGTTTA TTATGACGGT GGAACAGAAA
    CAGAGGTTAC ATAAATGTGC ATATTCCATA AGAGATTCAA AATTGAGAGA TCTCTTAATA
    CACGGTGTTG CTTATCATCA TGCTGGCATG GAGCTGTCAG ATAGAAAAGT AGTCGAAGGA
    GCTTTTACTG TTGGAGATCT ACCAGTTCTT TTTACTACTA GTACCTTAGC TATGGGAGTA
    AATTTGCCTG CTCATCTAGT AGTTATCAAA TCTACAATGC ATTATGCTGG AGGGATGTTT
    GAAGAGTACA GTGAAACAGA TATTCTACAG ATGATTGGTA GAGCTGGTCG ACCTCAATTT
    GACACTACAG CAACTGCAGT TATTATGACT CGGTTAAGTA CAAAAGAGAA GTATATTCAG
    ATGCTGGCTT GTAGTGACAC TGTAGAAAGC AGTTTGCACA GACATCTTAT TGAGCATTTA
    AATGCAGAGA TAGTGTTGCA TACCATCACA GATGTCAACA TTGCTTTGGA ATGGATACGA
    TCAACCTTGC TGTATATCAG AGCCTTGAAG AATCCGTCTC ACTATGGTTT TGCATCTGGA
    TTGAACAAAG ATGGAATTGA ATCAAAATTA CAAGAATTGT GTTTGAAGAA TTTGAATGAT
    TTATCATCTC TGGACTTGAT AAAGATGGAT GAAGATATTA ATTTCAAGCC AACTGAGGCA
    GGAAGATTGA TGGCTTGGTA CTATGTTACA TTTGAGACAG TGAAGAAATT TTGTACAATC
    AGTGGAAAAG AAACTTTATC AGATCTGGTC ACAATGATAG CCAGCTGCAA GGAATTTCTA
    GATGTACAAT TAAGGATAAA TGAAAAGAAA ACACTGAATA CTTTGAACAA AGATCCAAAT
    CGGATAACTA TCAGATTTCC AATGGAAGGA AAAATTAAAA CAAGAGAAAT GAAAGTAAAT
    TGTCTTATTC AGGCTCAGCT AGGATGCATT CCCATACAAG ACTTTGCTTT GACACAAGAT
    ACCTCAAAGA TTTTCAGGAA TGGCTCACGA ATTACGAGAT GGTTATCAGA TTTTGTGGCT
    GTTCAAGAAA AGTTTGCTGT GCTTTTGAAC AGTTTGATTT TAGCTAAATG TTTTAGATGT
    AAACTTTGGG AAAACTCTCT GCATGTATCC AAACAACTGG AAAAAATCGG TATAACATTA
    TCAAATGCTA TGGTAAATGC AGGTTTGACT TCCTTTAAAA AAATAGAAGA AACAGATGCA
    AGGGAGCTTG AACTGATTTT AAATAGACAT CCCCCTTTTG GAACTCAGAT AAAAGAAACA
    GTGATGTGTC TGCCAAAATA CGAACTTGAA GTGGAACAGA TTGCAAGATA CAGTGATACC
    ACGGCAGAAG TTTTAGTGAC TGTTATATTA AGAAACTTCG AACCACTGCA AACTAAAAGA
    ACAGCACCAG ATTCTCACTA TGTTACCTTA ATCATAGGCG ATGCAGATAA TCAGCTGATT
    TTTAAGCATA AAATTATGGA TTCTGTTTTG CTGAAATCTG GAAATTGGGC TAAAAAGATC
    GATGTGAAGA GAGCTCTGAA ATCTGAAGAT CTTAGCATAA ATCTCATTAG TTCTGAGTAT
    GTTGGACTTG ATATTCAGCA GAAATTTACA GTCTTTTACT TAGGACCCAA GAGATTTGGA
    AATCAAATAA TTATGCAAAA AAAATCAGAA ACAGAGATCT TCCATTCTAA ACGTTCAGAT
    GGATCTCCTG TAGCAGGTCC CATTAAAGGA ATGACTGCCT GCAGAAAGCC TGGGCACCGA
    GAGTGTAACC ATCACTGTAA AAAGAAACAC ACGTGTGGAC ATGACTGCTG TAAAACTGGA
    GTTGCCCAGA AGCCAGAAAT GAAAGAGTCA ACAATTTCTT CTTATTTATC TGATTTAAGA
    AACAGGAATG CAGTTTCATC TCTTCCTCCA GTTAAACGTC TAAAGATGCA GATGAATAAA
    TCTCAAAGTG TGGACCTTAA AGAGTTTGGT TTTACTCCAC AACCTTCTCT TCCCAGTCTG
    TCAAGGTCAG AATGTTTAAA CATACCTAAA TTGCCTGTAA TGGAGCAGAG GGGTCAGCAC
    GAGCTCTATG CAGAAGGTCA ACAACAAGAA CCACCGGGAT ATCAGGACAA AGGTTTTTCC
    AGACAGACCT CTTCTACAAC CTCAAATGAG AAAACCCCTC AAACAACACT GTTCAACAGA
    AATATTAAGG CAACTGGAAA CCAGAATTTG ACAGCTGTGG TTAAAAGTAA TTCTTCCTAT
    TCAGAAGTTT TGAATGTGAA CTTTGAATTG GGAAATGAAG TTTGGGATGA TTTTGATGAT
    GAAAACTTAA TAGAAATTGC TAGCTTTTCA GCTGATACTG AAAAGACAAA AGCAGGATTT
    GGAAACACTT TGAGTTCAAG TGCCAGGGAA AATAAGCTAC CCTTCCAAGA ATCAAAAAGC
    AAATTCCAAA GAAAAATGTC AAACAGTTTT GTTTCATCAC ATGAGAAGCC AGATATTTAT
    TTACCAAATT CTGCTCTGTC CAGGATCGGT GCATCCTCCA TGACAAAAAT ACCTCAACCA
    GCTGGAAATG TCCCTTTCCA AGAAGTAAAA CAACAGAATC TGTCACCAGA GATTGAAAAG
    CTATGCTTTG CTCACTCTGA AAAAATATCA AGCTTTTCAA ATTCTGTTAC GCAAGTGGAT
    TGCTTTATTA GAAACAGTGA ATATAAAAAG CAACCTGACT TCAGTAGGTA TCATCTTGAT
    GATGAAGCTG ATGAAATGAA GTCTTTTCTG GGAATATTTG ATGGTATTTT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe248247
    Clone ID Related Accession (Same CDS sequence) XM_013997388.2
    Accession Version XM_013997388.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4098bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product probable ATP-dependent DNA helicase HFM1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010446.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_013997388.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGCTGAGAT CACATGACTC CTTGTGTTCT TTGGATAATT TATTTTTTGA AAAAACAGAT 
    GAAGCTGAAA AATACCCAGA CAATGAAAAA TCATTAGATT GGTTTCTCCC ACCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAAGCA TTAGAGGAAG ACATGCAAAG TCATAAACTG
    TTAGGTCAGA AAAAGAGGTC AAAAATGTTA ACATCCAATT TAAAGATAAC TAATGAAGAC
    ACAAGTTATA CTTCACCAAC ACAAAAATTC CAGTGTGCTT TCCCTTCTAA TAAATACGAA
    GAAGGCGATC TAAATTTAGT AGGGGCAGGT AGCAATGACT TACATGTTGC TGGCAAGCTG
    ACTTATGGTT CTCCTCAGAA GTACAAAAGT CACATTGGCC CTGAGATAGC ACTTGAGAAA
    AATGTTCCAG ATGATGCGAA AGTAGTTAAT TTTGCATACG ATAAAGGAGA GAGCACATCG
    GCCTTCCGAA AAAGATTATT TAAAATATCT GATAGTATAC ATGGGAATGC TTGTTCTAAT
    GACATTGCAA ACTTGGACTC TGATATTGGC TCAGCGAAGA TTGCCCAAAT GGAAATGAGC
    GAAGGGAAAT CGTGGAAATG GAACAATAGT AATCAGAAAC CTCAATATTC AGAAATTAAC
    ATGTTTACAG CCAGTGATAC TTTTTCTGCT TCTAAAATCA GAGAAGGCAT ATTCAAAGCA
    CCATCTTTTT CAGTTGCATC CCAGCCTCAT GAAGTTCAAG GGATAATAGA AAATGGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATCCCG GCAAAATTTA GAAGTGTTTT CAAAGAATTC
    CCATATTTCA ACTATATCCA GTCTAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    GTTGTGGTTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAA TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGCAGTCA GCGTTTTGAT GACTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC GGGAGATACA GTCATGGATG ACCTGTTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCAACTGGT TCGACTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTT GTCAGCAGGA TGAAAACTGT GCAGTTTTTA
    TCTCATGCTG TGGAAAATCC CAGAAATATT ATTCCAATGA GATTTGTAGC TGTATCTGCA
    ACAATTCCAA ATGCTGAGGA TATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCCGCTATC
    TGTCTGAAAA TGGATGAGAA ACATAGACCA GTGAAGCTTC GGAAAGTGGT CCTTGGTTTT
    CCCTGCAGTA ATAACCAAAC TGAATTTAAG TTTGATTTAA CCCTCAACTA CAAAATTGCC
    AGTGTTGTAC AAACGTACTC CGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTACAAC AGGCTGCTTC CATTCTTGTG AAAGATGCCA AGTTTATTAT GACGGTGGAA
    CAGAAACAGA GGTTACATAA ATGTGCATAT TCCATAAGAG ATTCAAAATT GAGAGATCTC
    TTAATACACG GTGTTGCTTA TCATCATGCT GGCATGGAGC TGTCAGATAG AAAAGTAGTC
    GAAGGAGCTT TTACTGTTGG AGATCTACCA GTTCTTTTTA CTACTAGTAC CTTAGCTATG
    GGAGTAAATT TGCCTGCTCA TCTAGTAGTT ATCAAATCTA CAATGCATTA TGCTGGAGGG
    ATGTTTGAAG AGTACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCAAC TGCAGTTATT ATGACTCGGT TAAGTACAAA AGAGAAGTAT
    ATTCAGATGC TGGCTTGTAG TGACACTGTA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT GTTGCATACC ATCACAGATG TCAACATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTGTA TATCAGAGCC TTGAAGAATC CGTCTCACTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATTTG
    AATGATTTAT CATCTCTGGA CTTGATAAAG ATGGATGAAG ATATTAATTT CAAGCCAACT
    GAGGCAGGAA GATTGATGGC TTGGTACTAT GTTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATCAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG GATAAATGAA AAGAAAACAC TGAATACTTT GAACAAAGAT
    CCAAATCGGA TAACTATCAG ATTTCCAATG GAAGGAAAAA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCA TACAAGACTT TGCTTTGACA
    CAAGATACCT CAAAGATTTT CAGGAATGGC TCACGAATTA CGAGATGGTT ATCAGATTTT
    GTGGCTGTTC AAGAAAAGTT TGCTGTGCTT TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCTGCAT GTATCCAAAC AACTGGAAAA AATCGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AGCTTGAACT GATTTTAAAT AGACATCCCC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTGTCTGCC AAAATACGAA CTTGAAGTGG AACAGATTGC AAGATACAGT
    GATACCACGG CAGAAGTTTT AGTGACTGTT ATATTAAGAA ACTTCGAACC ACTGCAAACT
    AAAAGAACAG CACCAGATTC TCACTATGTT ACCTTAATCA TAGGCGATGC AGATAATCAG
    CTGATTTTTA AGCATAAAAT TATGGATTCT GTTTTGCTGA AATCTGGAAA TTGGGCTAAA
    AAGATCGATG TGAAGAGAGC TCTGAAATCT GAAGATCTTA GCATAAATCT CATTAGTTCT
    GAGTATGTTG GACTTGATAT TCAGCAGAAA TTTACAGTCT TTTACTTAGG ACCCAAGAGA
    TTTGGAAATC AAATAATTAT GCAAAAAAAA TCAGAAACAG AGATCTTCCA TTCTAAACGT
    TCAGATGGAT CTCCTGTAGC AGGTCCCATT AAAGGAATGA CTGCCTGCAG AAAGCCTGGG
    CACCGAGAGT GTAACCATCA CTGTAAAAAG AAACACACGT GTGGACATGA CTGCTGTAAA
    ACTGGAGTTG CCCAGAAGCC AGAAATGAAA GAGTCAACAA TTTCTTCTTA TTTATCTGAT
    TTAAGAAACA GGAATGCAGT TTCATCTCTT CCTCCAGTTA AACGTCTAAA GATGCAGATG
    AATAAATCTC AAAGTGTGGA CCTTAAAGAG TTTGGTTTTA CTCCACAACC TTCTCTTCCC
    AGTCTGTCAA GGTCAGAATG TTTAAACATA CCTAAATTGC CTGTAATGGA GCAGAGGGGT
    CAGCACGAGC TCTATGCAGA AGGTCAACAA CAAGAACCAC CGGGATATCA GGACAAAGGT
    TTTTCCAGAC AGACCTCTTC TACAACCTCA AATGAGAAAA CCCCTCAAAC AACACTGTTC
    AACAGAAATA TTAAGGCAAC TGGAAACCAG AATTTGACAG CTGTGGTTAA AAGTAATTCT
    TCCTATTCAG AAGTTTTGAA TGTGAACTTT GAATTGGGAA ATGAAGTTTG GGATGATTTT
    GATGATGAAA ACTTAATAGA AATTGCTAGC TTTTCAGCTG ATACTGAAAA GACAAAAGCA
    GTTTTGTTTC ATCACATGAG AAGCCAGATA TTTATTTACC AAATTCTGCT CTGTCCAGGA
    TCGGTGCATC CTCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013852842.2
    CDS103..4200
    Translation

    Target ORF information:

    RefSeq Version XM_013997388.2
    Organism Sus scrofa(pig)
    Definition Sus scrofa HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_013997388.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGCTGAGAT CACATGACTC CTTGTGTTCT TTGGATAATT TATTTTTTGA AAAAACAGAT 
    GAAGCTGAAA AATACCCAGA CAATGAAAAA TCATTAGATT GGTTTCTCCC ACCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAAGCA TTAGAGGAAG ACATGCAAAG TCATAAACTG
    TTAGGTCAGA AAAAGAGGTC AAAAATGTTA ACATCCAATT TAAAGATAAC TAATGAAGAC
    ACAAGTTATA CTTCACCAAC ACAAAAATTC CAGTGTGCTT TCCCTTCTAA TAAATACGAA
    GAAGGCGATC TAAATTTAGT AGGGGCAGGT AGCAATGACT TACATGTTGC TGGCAAGCTG
    ACTTATGGTT CTCCTCAGAA GTACAAAAGT CACATTGGCC CTGAGATAGC ACTTGAGAAA
    AATGTTCCAG ATGATGCGAA AGTAGTTAAT TTTGCATACG ATAAAGGAGA GAGCACATCG
    GCCTTCCGAA AAAGATTATT TAAAATATCT GATAGTATAC ATGGGAATGC TTGTTCTAAT
    GACATTGCAA ACTTGGACTC TGATATTGGC TCAGCGAAGA TTGCCCAAAT GGAAATGAGC
    GAAGGGAAAT CGTGGAAATG GAACAATAGT AATCAGAAAC CTCAATATTC AGAAATTAAC
    ATGTTTACAG CCAGTGATAC TTTTTCTGCT TCTAAAATCA GAGAAGGCAT ATTCAAAGCA
    CCATCTTTTT CAGTTGCATC CCAGCCTCAT GAAGTTCAAG GGATAATAGA AAATGGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATCCCG GCAAAATTTA GAAGTGTTTT CAAAGAATTC
    CCATATTTCA ACTATATCCA GTCTAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    GTTGTGGTTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAA TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGCAGTCA GCGTTTTGAT GACTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC GGGAGATACA GTCATGGATG ACCTGTTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCAACTGGT TCGACTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTT GTCAGCAGGA TGAAAACTGT GCAGTTTTTA
    TCTCATGCTG TGGAAAATCC CAGAAATATT ATTCCAATGA GATTTGTAGC TGTATCTGCA
    ACAATTCCAA ATGCTGAGGA TATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCCGCTATC
    TGTCTGAAAA TGGATGAGAA ACATAGACCA GTGAAGCTTC GGAAAGTGGT CCTTGGTTTT
    CCCTGCAGTA ATAACCAAAC TGAATTTAAG TTTGATTTAA CCCTCAACTA CAAAATTGCC
    AGTGTTGTAC AAACGTACTC CGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTACAAC AGGCTGCTTC CATTCTTGTG AAAGATGCCA AGTTTATTAT GACGGTGGAA
    CAGAAACAGA GGTTACATAA ATGTGCATAT TCCATAAGAG ATTCAAAATT GAGAGATCTC
    TTAATACACG GTGTTGCTTA TCATCATGCT GGCATGGAGC TGTCAGATAG AAAAGTAGTC
    GAAGGAGCTT TTACTGTTGG AGATCTACCA GTTCTTTTTA CTACTAGTAC CTTAGCTATG
    GGAGTAAATT TGCCTGCTCA TCTAGTAGTT ATCAAATCTA CAATGCATTA TGCTGGAGGG
    ATGTTTGAAG AGTACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCAAC TGCAGTTATT ATGACTCGGT TAAGTACAAA AGAGAAGTAT
    ATTCAGATGC TGGCTTGTAG TGACACTGTA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT GTTGCATACC ATCACAGATG TCAACATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTGTA TATCAGAGCC TTGAAGAATC CGTCTCACTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATTTG
    AATGATTTAT CATCTCTGGA CTTGATAAAG ATGGATGAAG ATATTAATTT CAAGCCAACT
    GAGGCAGGAA GATTGATGGC TTGGTACTAT GTTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATCAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG GATAAATGAA AAGAAAACAC TGAATACTTT GAACAAAGAT
    CCAAATCGGA TAACTATCAG ATTTCCAATG GAAGGAAAAA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCA TACAAGACTT TGCTTTGACA
    CAAGATACCT CAAAGATTTT CAGGAATGGC TCACGAATTA CGAGATGGTT ATCAGATTTT
    GTGGCTGTTC AAGAAAAGTT TGCTGTGCTT TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCTGCAT GTATCCAAAC AACTGGAAAA AATCGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AGCTTGAACT GATTTTAAAT AGACATCCCC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTGTCTGCC AAAATACGAA CTTGAAGTGG AACAGATTGC AAGATACAGT
    GATACCACGG CAGAAGTTTT AGTGACTGTT ATATTAAGAA ACTTCGAACC ACTGCAAACT
    AAAAGAACAG CACCAGATTC TCACTATGTT ACCTTAATCA TAGGCGATGC AGATAATCAG
    CTGATTTTTA AGCATAAAAT TATGGATTCT GTTTTGCTGA AATCTGGAAA TTGGGCTAAA
    AAGATCGATG TGAAGAGAGC TCTGAAATCT GAAGATCTTA GCATAAATCT CATTAGTTCT
    GAGTATGTTG GACTTGATAT TCAGCAGAAA TTTACAGTCT TTTACTTAGG ACCCAAGAGA
    TTTGGAAATC AAATAATTAT GCAAAAAAAA TCAGAAACAG AGATCTTCCA TTCTAAACGT
    TCAGATGGAT CTCCTGTAGC AGGTCCCATT AAAGGAATGA CTGCCTGCAG AAAGCCTGGG
    CACCGAGAGT GTAACCATCA CTGTAAAAAG AAACACACGT GTGGACATGA CTGCTGTAAA
    ACTGGAGTTG CCCAGAAGCC AGAAATGAAA GAGTCAACAA TTTCTTCTTA TTTATCTGAT
    TTAAGAAACA GGAATGCAGT TTCATCTCTT CCTCCAGTTA AACGTCTAAA GATGCAGATG
    AATAAATCTC AAAGTGTGGA CCTTAAAGAG TTTGGTTTTA CTCCACAACC TTCTCTTCCC
    AGTCTGTCAA GGTCAGAATG TTTAAACATA CCTAAATTGC CTGTAATGGA GCAGAGGGGT
    CAGCACGAGC TCTATGCAGA AGGTCAACAA CAAGAACCAC CGGGATATCA GGACAAAGGT
    TTTTCCAGAC AGACCTCTTC TACAACCTCA AATGAGAAAA CCCCTCAAAC AACACTGTTC
    AACAGAAATA TTAAGGCAAC TGGAAACCAG AATTTGACAG CTGTGGTTAA AAGTAATTCT
    TCCTATTCAG AAGTTTTGAA TGTGAACTTT GAATTGGGAA ATGAAGTTTG GGATGATTTT
    GATGATGAAA ACTTAATAGA AATTGCTAGC TTTTCAGCTG ATACTGAAAA GACAAAAGCA
    GTTTTGTTTC ATCACATGAG AAGCCAGATA TTTATTTACC AAATTCTGCT CTGTCCAGGA
    TCGGTGCATC CTCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe248248
    Clone ID Related Accession (Same CDS sequence) XM_021090258.1 , XM_021090260.1 , XM_021090259.1
    Accession Version XM_021090258.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4035bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product probable ATP-dependent DNA helicase HFM1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010446.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCTGAGAT CACATGACTC CTTGTGTTCT TTGGATAATT TATTTTTTGA AAAAACAGAT 
    GAAGCTGAAA AATTATTTAA AATATCTGAT AGTATACATG GGAATGCTTG TTCTAATGAC
    ATTGCAAACT TGGACTCTGA TATTGGCTCA GCGAAGATTG CCCAAATGGA AATGAGCGAA
    GGGAAATCGT GGAAATGGAA CAATAGTAAT CAGAAACCTC AATATTCAGA AATTAACATG
    TTTACAGCCA GTGATACTTT TTCTGCTTCT AAAATCAGAG AAGGCATATT CAAAGCACCA
    TCTTTTTCAG TTGCATCCCA GCCTCATGAA GTTCAAGGGA TAATAGAAAA TGGTTTAGAT
    TCCTTGAAGG CTGTCACAGA AATCCCGGCA AAATTTAGAA GTGTTTTCAA AGAATTCCCA
    TATTTCAACT ATATCCAGTC TAAAGCCTTT GATGATCTTC TTTATACAGA TAGGAATGTT
    GTGGTTTGTG CTCCAACTGG TTCTGGGAAA ACTGTAATGT TTGAACTAGC TATAACAAGA
    TTGTTAATGG AAGTACCATT GCCATGGTCA AACATTAAAA TTGTTTACAT GGCACCAATA
    AAAGCCCTGT GCAGTCAGCG TTTTGATGAC TGGAAAGAAA AATTTGGACC AATAGGATTG
    AATTGTAAAG AACTCACGGG AGATACAGTC ATGGATGACC TGTTTGAGAT TCAGCATGCC
    CATATTATTA TGACAACTCC AGAAAAATGG GATAGCATGA CTAGAAAATG GAGAGACAAC
    TCTTTGGTCC AACTGGTTCG ACTGTTTCTC ATTGATGAGG TACATGTTGT AAAAGATGAA
    AATCGTGGAC CAACTCTTGA AGTTGTTGTC AGCAGGATGA AAACTGTGCA GTTTTTATCT
    CATGCTGTGG AAAATCCCAG AAATATTATT CCAATGAGAT TTGTAGCTGT ATCTGCAACA
    ATTCCAAATG CTGAGGATAT TGCAGAATGG CTTTCAGATG GTGAAAGATC CGCTATCTGT
    CTGAAAATGG ATGAGAAACA TAGACCAGTG AAGCTTCGGA AAGTGGTCCT TGGTTTTCCC
    TGCAGTAATA ACCAAACTGA ATTTAAGTTT GATTTAACCC TCAACTACAA AATTGCCAGT
    GTTGTACAAA CGTACTCCGA TCAGAAACCC ACACTTGTGT TTTGTGCAAC AAGGAAAGGT
    GTACAACAGG CTGCTTCCAT TCTTGTGAAA GATGCCAAGT TTATTATGAC GGTGGAACAG
    AAACAGAGGT TACATAAATG TGCATATTCC ATAAGAGATT CAAAATTGAG AGATCTCTTA
    ATACACGGTG TTGCTTATCA TCATGCTGGC ATGGAGCTGT CAGATAGAAA AGTAGTCGAA
    GGAGCTTTTA CTGTTGGAGA TCTACCAGTT CTTTTTACTA CTAGTACCTT AGCTATGGGA
    GTAAATTTGC CTGCTCATCT AGTAGTTATC AAATCTACAA TGCATTATGC TGGAGGGATG
    TTTGAAGAGT ACAGTGAAAC AGATATTCTA CAGATGATTG GTAGAGCTGG TCGACCTCAA
    TTTGACACTA CAGCAACTGC AGTTATTATG ACTCGGTTAA GTACAAAAGA GAAGTATATT
    CAGATGCTGG CTTGTAGTGA CACTGTAGAA AGCAGTTTGC ACAGACATCT TATTGAGCAT
    TTAAATGCAG AGATAGTGTT GCATACCATC ACAGATGTCA ACATTGCTTT GGAATGGATA
    CGATCAACCT TGCTGTATAT CAGAGCCTTG AAGAATCCGT CTCACTATGG TTTTGCATCT
    GGATTGAACA AAGATGGAAT TGAATCAAAA TTACAAGAAT TGTGTTTGAA GAATTTGAAT
    GATTTATCAT CTCTGGACTT GATAAAGATG GATGAAGATA TTAATTTCAA GCCAACTGAG
    GCAGGAAGAT TGATGGCTTG GTACTATGTT ACATTTGAGA CAGTGAAGAA ATTTTGTACA
    ATCAGTGGAA AAGAAACTTT ATCAGATCTG GTCACAATGA TAGCCAGCTG CAAGGAATTT
    CTAGATGTAC AATTAAGGAT AAATGAAAAG AAAACACTGA ATACTTTGAA CAAAGATCCA
    AATCGGATAA CTATCAGATT TCCAATGGAA GGAAAAATTA AAACAAGAGA AATGAAAGTA
    AATTGTCTTA TTCAGGCTCA GCTAGGATGC ATTCCCATAC AAGACTTTGC TTTGACACAA
    GATACCTCAA AGATTTTCAG GAATGGCTCA CGAATTACGA GATGGTTATC AGATTTTGTG
    GCTGTTCAAG AAAAGTTTGC TGTGCTTTTG AACAGTTTGA TTTTAGCTAA ATGTTTTAGA
    TGTAAACTTT GGGAAAACTC TCTGCATGTA TCCAAACAAC TGGAAAAAAT CGGTATAACA
    TTATCAAATG CTATGGTAAA TGCAGGTTTG ACTTCCTTTA AAAAAATAGA AGAAACAGAT
    GCAAGGGAGC TTGAACTGAT TTTAAATAGA CATCCCCCTT TTGGAACTCA GATAAAAGAA
    ACAGTGATGT GTCTGCCAAA ATACGAACTT GAAGTGGAAC AGATTGCAAG ATACAGTGAT
    ACCACGGCAG AAGTTTTAGT GACTGTTATA TTAAGAAACT TCGAACCACT GCAAACTAAA
    AGAACAGCAC CAGATTCTCA CTATGTTACC TTAATCATAG GCGATGCAGA TAATCAGCTG
    ATTTTTAAGC ATAAAATTAT GGATTCTGTT TTGCTGAAAT CTGGAAATTG GGCTAAAAAG
    ATCGATGTGA AGAGAGCTCT GAAATCTGAA GATCTTAGCA TAAATCTCAT TAGTTCTGAG
    TATGTTGGAC TTGATATTCA GCAGAAATTT ACAGTCTTTT ACTTAGGACC CAAGAGATTT
    GGAAATCAAA TAATTATGCA AAAAAAATCA GAAACAGAGA TCTTCCATTC TAAACGTTCA
    GATGGATCTC CTGTAGCAGG TCCCATTAAA GGAATGACTG CCTGCAGAAA GCCTGGGCAC
    CGAGAGTGTA ACCATCACTG TAAAAAGAAA CACACGTGTG GACATGACTG CTGTAAAACT
    GGAGTTGCCC AGAAGCCAGA AATGAAAGAG TCAACAATTT CTTCTTATTT ATCTGATTTA
    AGAAACAGGA ATGCAGTTTC ATCTCTTCCT CCAGTTAAAC GTCTAAAGAT GCAGATGAAT
    AAATCTCAAA GTGTGGACCT TAAAGAGTTT GGTTTTACTC CACAACCTTC TCTTCCCAGT
    CTGTCAAGGT CAGAATGTTT AAACATACCT AAATTGCCTG TAATGGAGCA GAGGGGTCAG
    CACGAGCTCT ATGCAGAAGG TCAACAACAA GAACCACCGG GATATCAGGA CAAAGGTTTT
    TCCAGACAGA CCTCTTCTAC AACCTCAAAT GAGAAAACCC CTCAAACAAC ACTGTTCAAC
    AGAAATATTA AGGCAACTGG AAACCAGAAT TTGACAGCTG TGGTTAAAAG TAATTCTTCC
    TATTCAGAAG TTTTGAATGT GAACTTTGAA TTGGGAAATG AAGTTTGGGA TGATTTTGAT
    GATGAAAACT TAATAGAAAT TGCTAGCTTT TCAGCTGATA CTGAAAAGAC AAAAGCAGGA
    TTTGGAAACA CTTTGAGTTC AAGTGCCAGG GAAAATAAGC TACCCTTCCA AGAATCAAAA
    AGCAAATTCC AAAGAAAAAT GTCAAACAGT TTTGTTTCAT CACATGAGAA GCCAGATATT
    TATTTACCAA ATTCTGCTCT GTCCAGGATC GGTGCATCCT CCATGACAAA AATACCTCAA
    CCAGCTGGAA ATGTCCCTTT CCAAGAAGTA AAACAACAGA ATCTGTCACC AGAGATTGAA
    AAGCTATGCT TTGCTCACTC TGAAAAAATA TCAAGCTTTT CAAATTCTGT TACGCAAGTG
    GATTGCTTTA TTAGAAACAG TGAATATAAA AAGCAACCTG ACTTCAGTAA GTTATATTTC
    TTGCCTCGTC ATAAGAAACA ATATGGATTA TACGTTAAAT ATACAAGCTG CCAGACTTCA
    TCCCAGAGCT CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020945917.1
    CDS103..4137
    Translation

    Target ORF information:

    RefSeq Version XM_021090258.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021090258.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCTGAGAT CACATGACTC CTTGTGTTCT TTGGATAATT TATTTTTTGA AAAAACAGAT 
    GAAGCTGAAA AATTATTTAA AATATCTGAT AGTATACATG GGAATGCTTG TTCTAATGAC
    ATTGCAAACT TGGACTCTGA TATTGGCTCA GCGAAGATTG CCCAAATGGA AATGAGCGAA
    GGGAAATCGT GGAAATGGAA CAATAGTAAT CAGAAACCTC AATATTCAGA AATTAACATG
    TTTACAGCCA GTGATACTTT TTCTGCTTCT AAAATCAGAG AAGGCATATT CAAAGCACCA
    TCTTTTTCAG TTGCATCCCA GCCTCATGAA GTTCAAGGGA TAATAGAAAA TGGTTTAGAT
    TCCTTGAAGG CTGTCACAGA AATCCCGGCA AAATTTAGAA GTGTTTTCAA AGAATTCCCA
    TATTTCAACT ATATCCAGTC TAAAGCCTTT GATGATCTTC TTTATACAGA TAGGAATGTT
    GTGGTTTGTG CTCCAACTGG TTCTGGGAAA ACTGTAATGT TTGAACTAGC TATAACAAGA
    TTGTTAATGG AAGTACCATT GCCATGGTCA AACATTAAAA TTGTTTACAT GGCACCAATA
    AAAGCCCTGT GCAGTCAGCG TTTTGATGAC TGGAAAGAAA AATTTGGACC AATAGGATTG
    AATTGTAAAG AACTCACGGG AGATACAGTC ATGGATGACC TGTTTGAGAT TCAGCATGCC
    CATATTATTA TGACAACTCC AGAAAAATGG GATAGCATGA CTAGAAAATG GAGAGACAAC
    TCTTTGGTCC AACTGGTTCG ACTGTTTCTC ATTGATGAGG TACATGTTGT AAAAGATGAA
    AATCGTGGAC CAACTCTTGA AGTTGTTGTC AGCAGGATGA AAACTGTGCA GTTTTTATCT
    CATGCTGTGG AAAATCCCAG AAATATTATT CCAATGAGAT TTGTAGCTGT ATCTGCAACA
    ATTCCAAATG CTGAGGATAT TGCAGAATGG CTTTCAGATG GTGAAAGATC CGCTATCTGT
    CTGAAAATGG ATGAGAAACA TAGACCAGTG AAGCTTCGGA AAGTGGTCCT TGGTTTTCCC
    TGCAGTAATA ACCAAACTGA ATTTAAGTTT GATTTAACCC TCAACTACAA AATTGCCAGT
    GTTGTACAAA CGTACTCCGA TCAGAAACCC ACACTTGTGT TTTGTGCAAC AAGGAAAGGT
    GTACAACAGG CTGCTTCCAT TCTTGTGAAA GATGCCAAGT TTATTATGAC GGTGGAACAG
    AAACAGAGGT TACATAAATG TGCATATTCC ATAAGAGATT CAAAATTGAG AGATCTCTTA
    ATACACGGTG TTGCTTATCA TCATGCTGGC ATGGAGCTGT CAGATAGAAA AGTAGTCGAA
    GGAGCTTTTA CTGTTGGAGA TCTACCAGTT CTTTTTACTA CTAGTACCTT AGCTATGGGA
    GTAAATTTGC CTGCTCATCT AGTAGTTATC AAATCTACAA TGCATTATGC TGGAGGGATG
    TTTGAAGAGT ACAGTGAAAC AGATATTCTA CAGATGATTG GTAGAGCTGG TCGACCTCAA
    TTTGACACTA CAGCAACTGC AGTTATTATG ACTCGGTTAA GTACAAAAGA GAAGTATATT
    CAGATGCTGG CTTGTAGTGA CACTGTAGAA AGCAGTTTGC ACAGACATCT TATTGAGCAT
    TTAAATGCAG AGATAGTGTT GCATACCATC ACAGATGTCA ACATTGCTTT GGAATGGATA
    CGATCAACCT TGCTGTATAT CAGAGCCTTG AAGAATCCGT CTCACTATGG TTTTGCATCT
    GGATTGAACA AAGATGGAAT TGAATCAAAA TTACAAGAAT TGTGTTTGAA GAATTTGAAT
    GATTTATCAT CTCTGGACTT GATAAAGATG GATGAAGATA TTAATTTCAA GCCAACTGAG
    GCAGGAAGAT TGATGGCTTG GTACTATGTT ACATTTGAGA CAGTGAAGAA ATTTTGTACA
    ATCAGTGGAA AAGAAACTTT ATCAGATCTG GTCACAATGA TAGCCAGCTG CAAGGAATTT
    CTAGATGTAC AATTAAGGAT AAATGAAAAG AAAACACTGA ATACTTTGAA CAAAGATCCA
    AATCGGATAA CTATCAGATT TCCAATGGAA GGAAAAATTA AAACAAGAGA AATGAAAGTA
    AATTGTCTTA TTCAGGCTCA GCTAGGATGC ATTCCCATAC AAGACTTTGC TTTGACACAA
    GATACCTCAA AGATTTTCAG GAATGGCTCA CGAATTACGA GATGGTTATC AGATTTTGTG
    GCTGTTCAAG AAAAGTTTGC TGTGCTTTTG AACAGTTTGA TTTTAGCTAA ATGTTTTAGA
    TGTAAACTTT GGGAAAACTC TCTGCATGTA TCCAAACAAC TGGAAAAAAT CGGTATAACA
    TTATCAAATG CTATGGTAAA TGCAGGTTTG ACTTCCTTTA AAAAAATAGA AGAAACAGAT
    GCAAGGGAGC TTGAACTGAT TTTAAATAGA CATCCCCCTT TTGGAACTCA GATAAAAGAA
    ACAGTGATGT GTCTGCCAAA ATACGAACTT GAAGTGGAAC AGATTGCAAG ATACAGTGAT
    ACCACGGCAG AAGTTTTAGT GACTGTTATA TTAAGAAACT TCGAACCACT GCAAACTAAA
    AGAACAGCAC CAGATTCTCA CTATGTTACC TTAATCATAG GCGATGCAGA TAATCAGCTG
    ATTTTTAAGC ATAAAATTAT GGATTCTGTT TTGCTGAAAT CTGGAAATTG GGCTAAAAAG
    ATCGATGTGA AGAGAGCTCT GAAATCTGAA GATCTTAGCA TAAATCTCAT TAGTTCTGAG
    TATGTTGGAC TTGATATTCA GCAGAAATTT ACAGTCTTTT ACTTAGGACC CAAGAGATTT
    GGAAATCAAA TAATTATGCA AAAAAAATCA GAAACAGAGA TCTTCCATTC TAAACGTTCA
    GATGGATCTC CTGTAGCAGG TCCCATTAAA GGAATGACTG CCTGCAGAAA GCCTGGGCAC
    CGAGAGTGTA ACCATCACTG TAAAAAGAAA CACACGTGTG GACATGACTG CTGTAAAACT
    GGAGTTGCCC AGAAGCCAGA AATGAAAGAG TCAACAATTT CTTCTTATTT ATCTGATTTA
    AGAAACAGGA ATGCAGTTTC ATCTCTTCCT CCAGTTAAAC GTCTAAAGAT GCAGATGAAT
    AAATCTCAAA GTGTGGACCT TAAAGAGTTT GGTTTTACTC CACAACCTTC TCTTCCCAGT
    CTGTCAAGGT CAGAATGTTT AAACATACCT AAATTGCCTG TAATGGAGCA GAGGGGTCAG
    CACGAGCTCT ATGCAGAAGG TCAACAACAA GAACCACCGG GATATCAGGA CAAAGGTTTT
    TCCAGACAGA CCTCTTCTAC AACCTCAAAT GAGAAAACCC CTCAAACAAC ACTGTTCAAC
    AGAAATATTA AGGCAACTGG AAACCAGAAT TTGACAGCTG TGGTTAAAAG TAATTCTTCC
    TATTCAGAAG TTTTGAATGT GAACTTTGAA TTGGGAAATG AAGTTTGGGA TGATTTTGAT
    GATGAAAACT TAATAGAAAT TGCTAGCTTT TCAGCTGATA CTGAAAAGAC AAAAGCAGGA
    TTTGGAAACA CTTTGAGTTC AAGTGCCAGG GAAAATAAGC TACCCTTCCA AGAATCAAAA
    AGCAAATTCC AAAGAAAAAT GTCAAACAGT TTTGTTTCAT CACATGAGAA GCCAGATATT
    TATTTACCAA ATTCTGCTCT GTCCAGGATC GGTGCATCCT CCATGACAAA AATACCTCAA
    CCAGCTGGAA ATGTCCCTTT CCAAGAAGTA AAACAACAGA ATCTGTCACC AGAGATTGAA
    AAGCTATGCT TTGCTCACTC TGAAAAAATA TCAAGCTTTT CAAATTCTGT TACGCAAGTG
    GATTGCTTTA TTAGAAACAG TGAATATAAA AAGCAACCTG ACTTCAGTAA GTTATATTTC
    TTGCCTCGTC ATAAGAAACA ATATGGATTA TACGTTAAAT ATACAAGCTG CCAGACTTCA
    TCCCAGAGCT CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe248248
    Clone ID Related Accession (Same CDS sequence) XM_021090258.1 , XM_021090260.1 , XM_021090259.1
    Accession Version XM_021090260.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4035bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product probable ATP-dependent DNA helicase HFM1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010446.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCTGAGAT CACATGACTC CTTGTGTTCT TTGGATAATT TATTTTTTGA AAAAACAGAT 
    GAAGCTGAAA AATTATTTAA AATATCTGAT AGTATACATG GGAATGCTTG TTCTAATGAC
    ATTGCAAACT TGGACTCTGA TATTGGCTCA GCGAAGATTG CCCAAATGGA AATGAGCGAA
    GGGAAATCGT GGAAATGGAA CAATAGTAAT CAGAAACCTC AATATTCAGA AATTAACATG
    TTTACAGCCA GTGATACTTT TTCTGCTTCT AAAATCAGAG AAGGCATATT CAAAGCACCA
    TCTTTTTCAG TTGCATCCCA GCCTCATGAA GTTCAAGGGA TAATAGAAAA TGGTTTAGAT
    TCCTTGAAGG CTGTCACAGA AATCCCGGCA AAATTTAGAA GTGTTTTCAA AGAATTCCCA
    TATTTCAACT ATATCCAGTC TAAAGCCTTT GATGATCTTC TTTATACAGA TAGGAATGTT
    GTGGTTTGTG CTCCAACTGG TTCTGGGAAA ACTGTAATGT TTGAACTAGC TATAACAAGA
    TTGTTAATGG AAGTACCATT GCCATGGTCA AACATTAAAA TTGTTTACAT GGCACCAATA
    AAAGCCCTGT GCAGTCAGCG TTTTGATGAC TGGAAAGAAA AATTTGGACC AATAGGATTG
    AATTGTAAAG AACTCACGGG AGATACAGTC ATGGATGACC TGTTTGAGAT TCAGCATGCC
    CATATTATTA TGACAACTCC AGAAAAATGG GATAGCATGA CTAGAAAATG GAGAGACAAC
    TCTTTGGTCC AACTGGTTCG ACTGTTTCTC ATTGATGAGG TACATGTTGT AAAAGATGAA
    AATCGTGGAC CAACTCTTGA AGTTGTTGTC AGCAGGATGA AAACTGTGCA GTTTTTATCT
    CATGCTGTGG AAAATCCCAG AAATATTATT CCAATGAGAT TTGTAGCTGT ATCTGCAACA
    ATTCCAAATG CTGAGGATAT TGCAGAATGG CTTTCAGATG GTGAAAGATC CGCTATCTGT
    CTGAAAATGG ATGAGAAACA TAGACCAGTG AAGCTTCGGA AAGTGGTCCT TGGTTTTCCC
    TGCAGTAATA ACCAAACTGA ATTTAAGTTT GATTTAACCC TCAACTACAA AATTGCCAGT
    GTTGTACAAA CGTACTCCGA TCAGAAACCC ACACTTGTGT TTTGTGCAAC AAGGAAAGGT
    GTACAACAGG CTGCTTCCAT TCTTGTGAAA GATGCCAAGT TTATTATGAC GGTGGAACAG
    AAACAGAGGT TACATAAATG TGCATATTCC ATAAGAGATT CAAAATTGAG AGATCTCTTA
    ATACACGGTG TTGCTTATCA TCATGCTGGC ATGGAGCTGT CAGATAGAAA AGTAGTCGAA
    GGAGCTTTTA CTGTTGGAGA TCTACCAGTT CTTTTTACTA CTAGTACCTT AGCTATGGGA
    GTAAATTTGC CTGCTCATCT AGTAGTTATC AAATCTACAA TGCATTATGC TGGAGGGATG
    TTTGAAGAGT ACAGTGAAAC AGATATTCTA CAGATGATTG GTAGAGCTGG TCGACCTCAA
    TTTGACACTA CAGCAACTGC AGTTATTATG ACTCGGTTAA GTACAAAAGA GAAGTATATT
    CAGATGCTGG CTTGTAGTGA CACTGTAGAA AGCAGTTTGC ACAGACATCT TATTGAGCAT
    TTAAATGCAG AGATAGTGTT GCATACCATC ACAGATGTCA ACATTGCTTT GGAATGGATA
    CGATCAACCT TGCTGTATAT CAGAGCCTTG AAGAATCCGT CTCACTATGG TTTTGCATCT
    GGATTGAACA AAGATGGAAT TGAATCAAAA TTACAAGAAT TGTGTTTGAA GAATTTGAAT
    GATTTATCAT CTCTGGACTT GATAAAGATG GATGAAGATA TTAATTTCAA GCCAACTGAG
    GCAGGAAGAT TGATGGCTTG GTACTATGTT ACATTTGAGA CAGTGAAGAA ATTTTGTACA
    ATCAGTGGAA AAGAAACTTT ATCAGATCTG GTCACAATGA TAGCCAGCTG CAAGGAATTT
    CTAGATGTAC AATTAAGGAT AAATGAAAAG AAAACACTGA ATACTTTGAA CAAAGATCCA
    AATCGGATAA CTATCAGATT TCCAATGGAA GGAAAAATTA AAACAAGAGA AATGAAAGTA
    AATTGTCTTA TTCAGGCTCA GCTAGGATGC ATTCCCATAC AAGACTTTGC TTTGACACAA
    GATACCTCAA AGATTTTCAG GAATGGCTCA CGAATTACGA GATGGTTATC AGATTTTGTG
    GCTGTTCAAG AAAAGTTTGC TGTGCTTTTG AACAGTTTGA TTTTAGCTAA ATGTTTTAGA
    TGTAAACTTT GGGAAAACTC TCTGCATGTA TCCAAACAAC TGGAAAAAAT CGGTATAACA
    TTATCAAATG CTATGGTAAA TGCAGGTTTG ACTTCCTTTA AAAAAATAGA AGAAACAGAT
    GCAAGGGAGC TTGAACTGAT TTTAAATAGA CATCCCCCTT TTGGAACTCA GATAAAAGAA
    ACAGTGATGT GTCTGCCAAA ATACGAACTT GAAGTGGAAC AGATTGCAAG ATACAGTGAT
    ACCACGGCAG AAGTTTTAGT GACTGTTATA TTAAGAAACT TCGAACCACT GCAAACTAAA
    AGAACAGCAC CAGATTCTCA CTATGTTACC TTAATCATAG GCGATGCAGA TAATCAGCTG
    ATTTTTAAGC ATAAAATTAT GGATTCTGTT TTGCTGAAAT CTGGAAATTG GGCTAAAAAG
    ATCGATGTGA AGAGAGCTCT GAAATCTGAA GATCTTAGCA TAAATCTCAT TAGTTCTGAG
    TATGTTGGAC TTGATATTCA GCAGAAATTT ACAGTCTTTT ACTTAGGACC CAAGAGATTT
    GGAAATCAAA TAATTATGCA AAAAAAATCA GAAACAGAGA TCTTCCATTC TAAACGTTCA
    GATGGATCTC CTGTAGCAGG TCCCATTAAA GGAATGACTG CCTGCAGAAA GCCTGGGCAC
    CGAGAGTGTA ACCATCACTG TAAAAAGAAA CACACGTGTG GACATGACTG CTGTAAAACT
    GGAGTTGCCC AGAAGCCAGA AATGAAAGAG TCAACAATTT CTTCTTATTT ATCTGATTTA
    AGAAACAGGA ATGCAGTTTC ATCTCTTCCT CCAGTTAAAC GTCTAAAGAT GCAGATGAAT
    AAATCTCAAA GTGTGGACCT TAAAGAGTTT GGTTTTACTC CACAACCTTC TCTTCCCAGT
    CTGTCAAGGT CAGAATGTTT AAACATACCT AAATTGCCTG TAATGGAGCA GAGGGGTCAG
    CACGAGCTCT ATGCAGAAGG TCAACAACAA GAACCACCGG GATATCAGGA CAAAGGTTTT
    TCCAGACAGA CCTCTTCTAC AACCTCAAAT GAGAAAACCC CTCAAACAAC ACTGTTCAAC
    AGAAATATTA AGGCAACTGG AAACCAGAAT TTGACAGCTG TGGTTAAAAG TAATTCTTCC
    TATTCAGAAG TTTTGAATGT GAACTTTGAA TTGGGAAATG AAGTTTGGGA TGATTTTGAT
    GATGAAAACT TAATAGAAAT TGCTAGCTTT TCAGCTGATA CTGAAAAGAC AAAAGCAGGA
    TTTGGAAACA CTTTGAGTTC AAGTGCCAGG GAAAATAAGC TACCCTTCCA AGAATCAAAA
    AGCAAATTCC AAAGAAAAAT GTCAAACAGT TTTGTTTCAT CACATGAGAA GCCAGATATT
    TATTTACCAA ATTCTGCTCT GTCCAGGATC GGTGCATCCT CCATGACAAA AATACCTCAA
    CCAGCTGGAA ATGTCCCTTT CCAAGAAGTA AAACAACAGA ATCTGTCACC AGAGATTGAA
    AAGCTATGCT TTGCTCACTC TGAAAAAATA TCAAGCTTTT CAAATTCTGT TACGCAAGTG
    GATTGCTTTA TTAGAAACAG TGAATATAAA AAGCAACCTG ACTTCAGTAA GTTATATTTC
    TTGCCTCGTC ATAAGAAACA ATATGGATTA TACGTTAAAT ATACAAGCTG CCAGACTTCA
    TCCCAGAGCT CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020945919.1
    CDS1113..5147
    Translation

    Target ORF information:

    RefSeq Version XM_021090260.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021090260.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCTGAGAT CACATGACTC CTTGTGTTCT TTGGATAATT TATTTTTTGA AAAAACAGAT 
    GAAGCTGAAA AATTATTTAA AATATCTGAT AGTATACATG GGAATGCTTG TTCTAATGAC
    ATTGCAAACT TGGACTCTGA TATTGGCTCA GCGAAGATTG CCCAAATGGA AATGAGCGAA
    GGGAAATCGT GGAAATGGAA CAATAGTAAT CAGAAACCTC AATATTCAGA AATTAACATG
    TTTACAGCCA GTGATACTTT TTCTGCTTCT AAAATCAGAG AAGGCATATT CAAAGCACCA
    TCTTTTTCAG TTGCATCCCA GCCTCATGAA GTTCAAGGGA TAATAGAAAA TGGTTTAGAT
    TCCTTGAAGG CTGTCACAGA AATCCCGGCA AAATTTAGAA GTGTTTTCAA AGAATTCCCA
    TATTTCAACT ATATCCAGTC TAAAGCCTTT GATGATCTTC TTTATACAGA TAGGAATGTT
    GTGGTTTGTG CTCCAACTGG TTCTGGGAAA ACTGTAATGT TTGAACTAGC TATAACAAGA
    TTGTTAATGG AAGTACCATT GCCATGGTCA AACATTAAAA TTGTTTACAT GGCACCAATA
    AAAGCCCTGT GCAGTCAGCG TTTTGATGAC TGGAAAGAAA AATTTGGACC AATAGGATTG
    AATTGTAAAG AACTCACGGG AGATACAGTC ATGGATGACC TGTTTGAGAT TCAGCATGCC
    CATATTATTA TGACAACTCC AGAAAAATGG GATAGCATGA CTAGAAAATG GAGAGACAAC
    TCTTTGGTCC AACTGGTTCG ACTGTTTCTC ATTGATGAGG TACATGTTGT AAAAGATGAA
    AATCGTGGAC CAACTCTTGA AGTTGTTGTC AGCAGGATGA AAACTGTGCA GTTTTTATCT
    CATGCTGTGG AAAATCCCAG AAATATTATT CCAATGAGAT TTGTAGCTGT ATCTGCAACA
    ATTCCAAATG CTGAGGATAT TGCAGAATGG CTTTCAGATG GTGAAAGATC CGCTATCTGT
    CTGAAAATGG ATGAGAAACA TAGACCAGTG AAGCTTCGGA AAGTGGTCCT TGGTTTTCCC
    TGCAGTAATA ACCAAACTGA ATTTAAGTTT GATTTAACCC TCAACTACAA AATTGCCAGT
    GTTGTACAAA CGTACTCCGA TCAGAAACCC ACACTTGTGT TTTGTGCAAC AAGGAAAGGT
    GTACAACAGG CTGCTTCCAT TCTTGTGAAA GATGCCAAGT TTATTATGAC GGTGGAACAG
    AAACAGAGGT TACATAAATG TGCATATTCC ATAAGAGATT CAAAATTGAG AGATCTCTTA
    ATACACGGTG TTGCTTATCA TCATGCTGGC ATGGAGCTGT CAGATAGAAA AGTAGTCGAA
    GGAGCTTTTA CTGTTGGAGA TCTACCAGTT CTTTTTACTA CTAGTACCTT AGCTATGGGA
    GTAAATTTGC CTGCTCATCT AGTAGTTATC AAATCTACAA TGCATTATGC TGGAGGGATG
    TTTGAAGAGT ACAGTGAAAC AGATATTCTA CAGATGATTG GTAGAGCTGG TCGACCTCAA
    TTTGACACTA CAGCAACTGC AGTTATTATG ACTCGGTTAA GTACAAAAGA GAAGTATATT
    CAGATGCTGG CTTGTAGTGA CACTGTAGAA AGCAGTTTGC ACAGACATCT TATTGAGCAT
    TTAAATGCAG AGATAGTGTT GCATACCATC ACAGATGTCA ACATTGCTTT GGAATGGATA
    CGATCAACCT TGCTGTATAT CAGAGCCTTG AAGAATCCGT CTCACTATGG TTTTGCATCT
    GGATTGAACA AAGATGGAAT TGAATCAAAA TTACAAGAAT TGTGTTTGAA GAATTTGAAT
    GATTTATCAT CTCTGGACTT GATAAAGATG GATGAAGATA TTAATTTCAA GCCAACTGAG
    GCAGGAAGAT TGATGGCTTG GTACTATGTT ACATTTGAGA CAGTGAAGAA ATTTTGTACA
    ATCAGTGGAA AAGAAACTTT ATCAGATCTG GTCACAATGA TAGCCAGCTG CAAGGAATTT
    CTAGATGTAC AATTAAGGAT AAATGAAAAG AAAACACTGA ATACTTTGAA CAAAGATCCA
    AATCGGATAA CTATCAGATT TCCAATGGAA GGAAAAATTA AAACAAGAGA AATGAAAGTA
    AATTGTCTTA TTCAGGCTCA GCTAGGATGC ATTCCCATAC AAGACTTTGC TTTGACACAA
    GATACCTCAA AGATTTTCAG GAATGGCTCA CGAATTACGA GATGGTTATC AGATTTTGTG
    GCTGTTCAAG AAAAGTTTGC TGTGCTTTTG AACAGTTTGA TTTTAGCTAA ATGTTTTAGA
    TGTAAACTTT GGGAAAACTC TCTGCATGTA TCCAAACAAC TGGAAAAAAT CGGTATAACA
    TTATCAAATG CTATGGTAAA TGCAGGTTTG ACTTCCTTTA AAAAAATAGA AGAAACAGAT
    GCAAGGGAGC TTGAACTGAT TTTAAATAGA CATCCCCCTT TTGGAACTCA GATAAAAGAA
    ACAGTGATGT GTCTGCCAAA ATACGAACTT GAAGTGGAAC AGATTGCAAG ATACAGTGAT
    ACCACGGCAG AAGTTTTAGT GACTGTTATA TTAAGAAACT TCGAACCACT GCAAACTAAA
    AGAACAGCAC CAGATTCTCA CTATGTTACC TTAATCATAG GCGATGCAGA TAATCAGCTG
    ATTTTTAAGC ATAAAATTAT GGATTCTGTT TTGCTGAAAT CTGGAAATTG GGCTAAAAAG
    ATCGATGTGA AGAGAGCTCT GAAATCTGAA GATCTTAGCA TAAATCTCAT TAGTTCTGAG
    TATGTTGGAC TTGATATTCA GCAGAAATTT ACAGTCTTTT ACTTAGGACC CAAGAGATTT
    GGAAATCAAA TAATTATGCA AAAAAAATCA GAAACAGAGA TCTTCCATTC TAAACGTTCA
    GATGGATCTC CTGTAGCAGG TCCCATTAAA GGAATGACTG CCTGCAGAAA GCCTGGGCAC
    CGAGAGTGTA ACCATCACTG TAAAAAGAAA CACACGTGTG GACATGACTG CTGTAAAACT
    GGAGTTGCCC AGAAGCCAGA AATGAAAGAG TCAACAATTT CTTCTTATTT ATCTGATTTA
    AGAAACAGGA ATGCAGTTTC ATCTCTTCCT CCAGTTAAAC GTCTAAAGAT GCAGATGAAT
    AAATCTCAAA GTGTGGACCT TAAAGAGTTT GGTTTTACTC CACAACCTTC TCTTCCCAGT
    CTGTCAAGGT CAGAATGTTT AAACATACCT AAATTGCCTG TAATGGAGCA GAGGGGTCAG
    CACGAGCTCT ATGCAGAAGG TCAACAACAA GAACCACCGG GATATCAGGA CAAAGGTTTT
    TCCAGACAGA CCTCTTCTAC AACCTCAAAT GAGAAAACCC CTCAAACAAC ACTGTTCAAC
    AGAAATATTA AGGCAACTGG AAACCAGAAT TTGACAGCTG TGGTTAAAAG TAATTCTTCC
    TATTCAGAAG TTTTGAATGT GAACTTTGAA TTGGGAAATG AAGTTTGGGA TGATTTTGAT
    GATGAAAACT TAATAGAAAT TGCTAGCTTT TCAGCTGATA CTGAAAAGAC AAAAGCAGGA
    TTTGGAAACA CTTTGAGTTC AAGTGCCAGG GAAAATAAGC TACCCTTCCA AGAATCAAAA
    AGCAAATTCC AAAGAAAAAT GTCAAACAGT TTTGTTTCAT CACATGAGAA GCCAGATATT
    TATTTACCAA ATTCTGCTCT GTCCAGGATC GGTGCATCCT CCATGACAAA AATACCTCAA
    CCAGCTGGAA ATGTCCCTTT CCAAGAAGTA AAACAACAGA ATCTGTCACC AGAGATTGAA
    AAGCTATGCT TTGCTCACTC TGAAAAAATA TCAAGCTTTT CAAATTCTGT TACGCAAGTG
    GATTGCTTTA TTAGAAACAG TGAATATAAA AAGCAACCTG ACTTCAGTAA GTTATATTTC
    TTGCCTCGTC ATAAGAAACA ATATGGATTA TACGTTAAAT ATACAAGCTG CCAGACTTCA
    TCCCAGAGCT CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe248248
    Clone ID Related Accession (Same CDS sequence) XM_021090258.1 , XM_021090260.1 , XM_021090259.1
    Accession Version XM_021090259.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4035bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product probable ATP-dependent DNA helicase HFM1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010446.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCTGAGAT CACATGACTC CTTGTGTTCT TTGGATAATT TATTTTTTGA AAAAACAGAT 
    GAAGCTGAAA AATTATTTAA AATATCTGAT AGTATACATG GGAATGCTTG TTCTAATGAC
    ATTGCAAACT TGGACTCTGA TATTGGCTCA GCGAAGATTG CCCAAATGGA AATGAGCGAA
    GGGAAATCGT GGAAATGGAA CAATAGTAAT CAGAAACCTC AATATTCAGA AATTAACATG
    TTTACAGCCA GTGATACTTT TTCTGCTTCT AAAATCAGAG AAGGCATATT CAAAGCACCA
    TCTTTTTCAG TTGCATCCCA GCCTCATGAA GTTCAAGGGA TAATAGAAAA TGGTTTAGAT
    TCCTTGAAGG CTGTCACAGA AATCCCGGCA AAATTTAGAA GTGTTTTCAA AGAATTCCCA
    TATTTCAACT ATATCCAGTC TAAAGCCTTT GATGATCTTC TTTATACAGA TAGGAATGTT
    GTGGTTTGTG CTCCAACTGG TTCTGGGAAA ACTGTAATGT TTGAACTAGC TATAACAAGA
    TTGTTAATGG AAGTACCATT GCCATGGTCA AACATTAAAA TTGTTTACAT GGCACCAATA
    AAAGCCCTGT GCAGTCAGCG TTTTGATGAC TGGAAAGAAA AATTTGGACC AATAGGATTG
    AATTGTAAAG AACTCACGGG AGATACAGTC ATGGATGACC TGTTTGAGAT TCAGCATGCC
    CATATTATTA TGACAACTCC AGAAAAATGG GATAGCATGA CTAGAAAATG GAGAGACAAC
    TCTTTGGTCC AACTGGTTCG ACTGTTTCTC ATTGATGAGG TACATGTTGT AAAAGATGAA
    AATCGTGGAC CAACTCTTGA AGTTGTTGTC AGCAGGATGA AAACTGTGCA GTTTTTATCT
    CATGCTGTGG AAAATCCCAG AAATATTATT CCAATGAGAT TTGTAGCTGT ATCTGCAACA
    ATTCCAAATG CTGAGGATAT TGCAGAATGG CTTTCAGATG GTGAAAGATC CGCTATCTGT
    CTGAAAATGG ATGAGAAACA TAGACCAGTG AAGCTTCGGA AAGTGGTCCT TGGTTTTCCC
    TGCAGTAATA ACCAAACTGA ATTTAAGTTT GATTTAACCC TCAACTACAA AATTGCCAGT
    GTTGTACAAA CGTACTCCGA TCAGAAACCC ACACTTGTGT TTTGTGCAAC AAGGAAAGGT
    GTACAACAGG CTGCTTCCAT TCTTGTGAAA GATGCCAAGT TTATTATGAC GGTGGAACAG
    AAACAGAGGT TACATAAATG TGCATATTCC ATAAGAGATT CAAAATTGAG AGATCTCTTA
    ATACACGGTG TTGCTTATCA TCATGCTGGC ATGGAGCTGT CAGATAGAAA AGTAGTCGAA
    GGAGCTTTTA CTGTTGGAGA TCTACCAGTT CTTTTTACTA CTAGTACCTT AGCTATGGGA
    GTAAATTTGC CTGCTCATCT AGTAGTTATC AAATCTACAA TGCATTATGC TGGAGGGATG
    TTTGAAGAGT ACAGTGAAAC AGATATTCTA CAGATGATTG GTAGAGCTGG TCGACCTCAA
    TTTGACACTA CAGCAACTGC AGTTATTATG ACTCGGTTAA GTACAAAAGA GAAGTATATT
    CAGATGCTGG CTTGTAGTGA CACTGTAGAA AGCAGTTTGC ACAGACATCT TATTGAGCAT
    TTAAATGCAG AGATAGTGTT GCATACCATC ACAGATGTCA ACATTGCTTT GGAATGGATA
    CGATCAACCT TGCTGTATAT CAGAGCCTTG AAGAATCCGT CTCACTATGG TTTTGCATCT
    GGATTGAACA AAGATGGAAT TGAATCAAAA TTACAAGAAT TGTGTTTGAA GAATTTGAAT
    GATTTATCAT CTCTGGACTT GATAAAGATG GATGAAGATA TTAATTTCAA GCCAACTGAG
    GCAGGAAGAT TGATGGCTTG GTACTATGTT ACATTTGAGA CAGTGAAGAA ATTTTGTACA
    ATCAGTGGAA AAGAAACTTT ATCAGATCTG GTCACAATGA TAGCCAGCTG CAAGGAATTT
    CTAGATGTAC AATTAAGGAT AAATGAAAAG AAAACACTGA ATACTTTGAA CAAAGATCCA
    AATCGGATAA CTATCAGATT TCCAATGGAA GGAAAAATTA AAACAAGAGA AATGAAAGTA
    AATTGTCTTA TTCAGGCTCA GCTAGGATGC ATTCCCATAC AAGACTTTGC TTTGACACAA
    GATACCTCAA AGATTTTCAG GAATGGCTCA CGAATTACGA GATGGTTATC AGATTTTGTG
    GCTGTTCAAG AAAAGTTTGC TGTGCTTTTG AACAGTTTGA TTTTAGCTAA ATGTTTTAGA
    TGTAAACTTT GGGAAAACTC TCTGCATGTA TCCAAACAAC TGGAAAAAAT CGGTATAACA
    TTATCAAATG CTATGGTAAA TGCAGGTTTG ACTTCCTTTA AAAAAATAGA AGAAACAGAT
    GCAAGGGAGC TTGAACTGAT TTTAAATAGA CATCCCCCTT TTGGAACTCA GATAAAAGAA
    ACAGTGATGT GTCTGCCAAA ATACGAACTT GAAGTGGAAC AGATTGCAAG ATACAGTGAT
    ACCACGGCAG AAGTTTTAGT GACTGTTATA TTAAGAAACT TCGAACCACT GCAAACTAAA
    AGAACAGCAC CAGATTCTCA CTATGTTACC TTAATCATAG GCGATGCAGA TAATCAGCTG
    ATTTTTAAGC ATAAAATTAT GGATTCTGTT TTGCTGAAAT CTGGAAATTG GGCTAAAAAG
    ATCGATGTGA AGAGAGCTCT GAAATCTGAA GATCTTAGCA TAAATCTCAT TAGTTCTGAG
    TATGTTGGAC TTGATATTCA GCAGAAATTT ACAGTCTTTT ACTTAGGACC CAAGAGATTT
    GGAAATCAAA TAATTATGCA AAAAAAATCA GAAACAGAGA TCTTCCATTC TAAACGTTCA
    GATGGATCTC CTGTAGCAGG TCCCATTAAA GGAATGACTG CCTGCAGAAA GCCTGGGCAC
    CGAGAGTGTA ACCATCACTG TAAAAAGAAA CACACGTGTG GACATGACTG CTGTAAAACT
    GGAGTTGCCC AGAAGCCAGA AATGAAAGAG TCAACAATTT CTTCTTATTT ATCTGATTTA
    AGAAACAGGA ATGCAGTTTC ATCTCTTCCT CCAGTTAAAC GTCTAAAGAT GCAGATGAAT
    AAATCTCAAA GTGTGGACCT TAAAGAGTTT GGTTTTACTC CACAACCTTC TCTTCCCAGT
    CTGTCAAGGT CAGAATGTTT AAACATACCT AAATTGCCTG TAATGGAGCA GAGGGGTCAG
    CACGAGCTCT ATGCAGAAGG TCAACAACAA GAACCACCGG GATATCAGGA CAAAGGTTTT
    TCCAGACAGA CCTCTTCTAC AACCTCAAAT GAGAAAACCC CTCAAACAAC ACTGTTCAAC
    AGAAATATTA AGGCAACTGG AAACCAGAAT TTGACAGCTG TGGTTAAAAG TAATTCTTCC
    TATTCAGAAG TTTTGAATGT GAACTTTGAA TTGGGAAATG AAGTTTGGGA TGATTTTGAT
    GATGAAAACT TAATAGAAAT TGCTAGCTTT TCAGCTGATA CTGAAAAGAC AAAAGCAGGA
    TTTGGAAACA CTTTGAGTTC AAGTGCCAGG GAAAATAAGC TACCCTTCCA AGAATCAAAA
    AGCAAATTCC AAAGAAAAAT GTCAAACAGT TTTGTTTCAT CACATGAGAA GCCAGATATT
    TATTTACCAA ATTCTGCTCT GTCCAGGATC GGTGCATCCT CCATGACAAA AATACCTCAA
    CCAGCTGGAA ATGTCCCTTT CCAAGAAGTA AAACAACAGA ATCTGTCACC AGAGATTGAA
    AAGCTATGCT TTGCTCACTC TGAAAAAATA TCAAGCTTTT CAAATTCTGT TACGCAAGTG
    GATTGCTTTA TTAGAAACAG TGAATATAAA AAGCAACCTG ACTTCAGTAA GTTATATTTC
    TTGCCTCGTC ATAAGAAACA ATATGGATTA TACGTTAAAT ATACAAGCTG CCAGACTTCA
    TCCCAGAGCT CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020945918.1
    CDS397..4431
    Translation

    Target ORF information:

    RefSeq Version XM_021090259.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021090259.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCTGAGAT CACATGACTC CTTGTGTTCT TTGGATAATT TATTTTTTGA AAAAACAGAT 
    GAAGCTGAAA AATTATTTAA AATATCTGAT AGTATACATG GGAATGCTTG TTCTAATGAC
    ATTGCAAACT TGGACTCTGA TATTGGCTCA GCGAAGATTG CCCAAATGGA AATGAGCGAA
    GGGAAATCGT GGAAATGGAA CAATAGTAAT CAGAAACCTC AATATTCAGA AATTAACATG
    TTTACAGCCA GTGATACTTT TTCTGCTTCT AAAATCAGAG AAGGCATATT CAAAGCACCA
    TCTTTTTCAG TTGCATCCCA GCCTCATGAA GTTCAAGGGA TAATAGAAAA TGGTTTAGAT
    TCCTTGAAGG CTGTCACAGA AATCCCGGCA AAATTTAGAA GTGTTTTCAA AGAATTCCCA
    TATTTCAACT ATATCCAGTC TAAAGCCTTT GATGATCTTC TTTATACAGA TAGGAATGTT
    GTGGTTTGTG CTCCAACTGG TTCTGGGAAA ACTGTAATGT TTGAACTAGC TATAACAAGA
    TTGTTAATGG AAGTACCATT GCCATGGTCA AACATTAAAA TTGTTTACAT GGCACCAATA
    AAAGCCCTGT GCAGTCAGCG TTTTGATGAC TGGAAAGAAA AATTTGGACC AATAGGATTG
    AATTGTAAAG AACTCACGGG AGATACAGTC ATGGATGACC TGTTTGAGAT TCAGCATGCC
    CATATTATTA TGACAACTCC AGAAAAATGG GATAGCATGA CTAGAAAATG GAGAGACAAC
    TCTTTGGTCC AACTGGTTCG ACTGTTTCTC ATTGATGAGG TACATGTTGT AAAAGATGAA
    AATCGTGGAC CAACTCTTGA AGTTGTTGTC AGCAGGATGA AAACTGTGCA GTTTTTATCT
    CATGCTGTGG AAAATCCCAG AAATATTATT CCAATGAGAT TTGTAGCTGT ATCTGCAACA
    ATTCCAAATG CTGAGGATAT TGCAGAATGG CTTTCAGATG GTGAAAGATC CGCTATCTGT
    CTGAAAATGG ATGAGAAACA TAGACCAGTG AAGCTTCGGA AAGTGGTCCT TGGTTTTCCC
    TGCAGTAATA ACCAAACTGA ATTTAAGTTT GATTTAACCC TCAACTACAA AATTGCCAGT
    GTTGTACAAA CGTACTCCGA TCAGAAACCC ACACTTGTGT TTTGTGCAAC AAGGAAAGGT
    GTACAACAGG CTGCTTCCAT TCTTGTGAAA GATGCCAAGT TTATTATGAC GGTGGAACAG
    AAACAGAGGT TACATAAATG TGCATATTCC ATAAGAGATT CAAAATTGAG AGATCTCTTA
    ATACACGGTG TTGCTTATCA TCATGCTGGC ATGGAGCTGT CAGATAGAAA AGTAGTCGAA
    GGAGCTTTTA CTGTTGGAGA TCTACCAGTT CTTTTTACTA CTAGTACCTT AGCTATGGGA
    GTAAATTTGC CTGCTCATCT AGTAGTTATC AAATCTACAA TGCATTATGC TGGAGGGATG
    TTTGAAGAGT ACAGTGAAAC AGATATTCTA CAGATGATTG GTAGAGCTGG TCGACCTCAA
    TTTGACACTA CAGCAACTGC AGTTATTATG ACTCGGTTAA GTACAAAAGA GAAGTATATT
    CAGATGCTGG CTTGTAGTGA CACTGTAGAA AGCAGTTTGC ACAGACATCT TATTGAGCAT
    TTAAATGCAG AGATAGTGTT GCATACCATC ACAGATGTCA ACATTGCTTT GGAATGGATA
    CGATCAACCT TGCTGTATAT CAGAGCCTTG AAGAATCCGT CTCACTATGG TTTTGCATCT
    GGATTGAACA AAGATGGAAT TGAATCAAAA TTACAAGAAT TGTGTTTGAA GAATTTGAAT
    GATTTATCAT CTCTGGACTT GATAAAGATG GATGAAGATA TTAATTTCAA GCCAACTGAG
    GCAGGAAGAT TGATGGCTTG GTACTATGTT ACATTTGAGA CAGTGAAGAA ATTTTGTACA
    ATCAGTGGAA AAGAAACTTT ATCAGATCTG GTCACAATGA TAGCCAGCTG CAAGGAATTT
    CTAGATGTAC AATTAAGGAT AAATGAAAAG AAAACACTGA ATACTTTGAA CAAAGATCCA
    AATCGGATAA CTATCAGATT TCCAATGGAA GGAAAAATTA AAACAAGAGA AATGAAAGTA
    AATTGTCTTA TTCAGGCTCA GCTAGGATGC ATTCCCATAC AAGACTTTGC TTTGACACAA
    GATACCTCAA AGATTTTCAG GAATGGCTCA CGAATTACGA GATGGTTATC AGATTTTGTG
    GCTGTTCAAG AAAAGTTTGC TGTGCTTTTG AACAGTTTGA TTTTAGCTAA ATGTTTTAGA
    TGTAAACTTT GGGAAAACTC TCTGCATGTA TCCAAACAAC TGGAAAAAAT CGGTATAACA
    TTATCAAATG CTATGGTAAA TGCAGGTTTG ACTTCCTTTA AAAAAATAGA AGAAACAGAT
    GCAAGGGAGC TTGAACTGAT TTTAAATAGA CATCCCCCTT TTGGAACTCA GATAAAAGAA
    ACAGTGATGT GTCTGCCAAA ATACGAACTT GAAGTGGAAC AGATTGCAAG ATACAGTGAT
    ACCACGGCAG AAGTTTTAGT GACTGTTATA TTAAGAAACT TCGAACCACT GCAAACTAAA
    AGAACAGCAC CAGATTCTCA CTATGTTACC TTAATCATAG GCGATGCAGA TAATCAGCTG
    ATTTTTAAGC ATAAAATTAT GGATTCTGTT TTGCTGAAAT CTGGAAATTG GGCTAAAAAG
    ATCGATGTGA AGAGAGCTCT GAAATCTGAA GATCTTAGCA TAAATCTCAT TAGTTCTGAG
    TATGTTGGAC TTGATATTCA GCAGAAATTT ACAGTCTTTT ACTTAGGACC CAAGAGATTT
    GGAAATCAAA TAATTATGCA AAAAAAATCA GAAACAGAGA TCTTCCATTC TAAACGTTCA
    GATGGATCTC CTGTAGCAGG TCCCATTAAA GGAATGACTG CCTGCAGAAA GCCTGGGCAC
    CGAGAGTGTA ACCATCACTG TAAAAAGAAA CACACGTGTG GACATGACTG CTGTAAAACT
    GGAGTTGCCC AGAAGCCAGA AATGAAAGAG TCAACAATTT CTTCTTATTT ATCTGATTTA
    AGAAACAGGA ATGCAGTTTC ATCTCTTCCT CCAGTTAAAC GTCTAAAGAT GCAGATGAAT
    AAATCTCAAA GTGTGGACCT TAAAGAGTTT GGTTTTACTC CACAACCTTC TCTTCCCAGT
    CTGTCAAGGT CAGAATGTTT AAACATACCT AAATTGCCTG TAATGGAGCA GAGGGGTCAG
    CACGAGCTCT ATGCAGAAGG TCAACAACAA GAACCACCGG GATATCAGGA CAAAGGTTTT
    TCCAGACAGA CCTCTTCTAC AACCTCAAAT GAGAAAACCC CTCAAACAAC ACTGTTCAAC
    AGAAATATTA AGGCAACTGG AAACCAGAAT TTGACAGCTG TGGTTAAAAG TAATTCTTCC
    TATTCAGAAG TTTTGAATGT GAACTTTGAA TTGGGAAATG AAGTTTGGGA TGATTTTGAT
    GATGAAAACT TAATAGAAAT TGCTAGCTTT TCAGCTGATA CTGAAAAGAC AAAAGCAGGA
    TTTGGAAACA CTTTGAGTTC AAGTGCCAGG GAAAATAAGC TACCCTTCCA AGAATCAAAA
    AGCAAATTCC AAAGAAAAAT GTCAAACAGT TTTGTTTCAT CACATGAGAA GCCAGATATT
    TATTTACCAA ATTCTGCTCT GTCCAGGATC GGTGCATCCT CCATGACAAA AATACCTCAA
    CCAGCTGGAA ATGTCCCTTT CCAAGAAGTA AAACAACAGA ATCTGTCACC AGAGATTGAA
    AAGCTATGCT TTGCTCACTC TGAAAAAATA TCAAGCTTTT CAAATTCTGT TACGCAAGTG
    GATTGCTTTA TTAGAAACAG TGAATATAAA AAGCAACCTG ACTTCAGTAA GTTATATTTC
    TTGCCTCGTC ATAAGAAACA ATATGGATTA TACGTTAAAT ATACAAGCTG CCAGACTTCA
    TCCCAGAGCT CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe35333
    Clone ID Related Accession (Same CDS sequence) XM_013997383.1 , XM_013997384.1 , XM_013997385.1
    Accession Version XM_013997383.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4440bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-10
    Organism Sus scrofa(Pig)
    Product probable ATP-dependent DNA helicase HFM1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003610184.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCTGAGAT CACATGACTC CTTGTGTTCT TTGGATAATT TATTTTTTGA AAAAACAGAT 
    GAAGCTGAAA AATACCCAGA CAATGAAAAA TCATTAGATT GGTTTCTCCC ACCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAAGCA TTAGAGGAAG ACATGCAAAG TCATAAACTG
    TTAGGTCAGA AAAAGAGGTC AAAAATGTTA ACATCCAATT TAAAGATAAC TAATGAAGAC
    ACAAGTTATA CTTCACCAAC ACAAAAATTC CAGTGTGCTT TCCCTTCTAA TAAATACGAA
    GAAGGCGATC TAAATTTAGT AGGGGCAGGT AGCAATGACT TACATGTTGC TGGCAAGCTG
    ACTTATGGTT CTCCTCAGAA GTACAAAAGT CACATTGGCC CTGAGATAGC ACTTGAGAAA
    AATGTTCCAG ATGATGCGAA AGTAGTTAAT TTTGCATACG ATAAAGGAGA GAGCACATCG
    GCCTTCCGAA AAAGATTATT TAAAATATCT GATAGTATAC ATGGGAATGC TTGTTCTAAT
    GACATTGCAA ACTTGGACTC TGATATTGGC TCAGCGAAGA TTGCCCAAAT GGAAATGAGC
    GAAGGGAAAT CGTGGAAATG GAACAATAGT AATCAGAAAC CTCAATATTC AGAAATTAAC
    ATGTTTACAG CCAGTGATAC TTTTTCTGCT TCTAAAATCA GAGAAGGCAT ATTCAAAGCA
    CCATCTTTTT CAGTTGCATC CCAGCCTCAT GAAGTTCAAG GGATAATAGA AAATGGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATCCCG GCAAAATTTA GAAGTGTTTT CAAAGAATTC
    CCATATTTCA ACTATATCCA GTCTAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    GTTGTGGTTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAA TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGCAGTCA GCGTTTTGAT GACTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC GGGAGATACA GTCATGGATG ACCTGTTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCAACTGGT TCGACTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTT GTCAGCAGGA TGAAAACTGT GCAGTTTTTA
    TCTCATGCTG TGGAAAATCC CAGAAATATT ATTCCAATGA GATTTGTAGC TGTATCTGCA
    ACAATTCCAA ATGCTGAGGA TATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCCGCTATC
    TGTCTGAAAA TGGATGAGAA ACATAGACCA GTGAAGCTTC GGAAAGTGGT CCTTGGTTTT
    CCCTGCAGTA ATAACCAAAC TGAATTTAAG TTTGATTTAA CCCTCAACTA CAAAATTGCC
    AGTGTTGTAC AAACGTACTC CGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTACAAC AGGCTGCTTC CATTCTTGTG AAAGATGCCA AGTTTATTAT GACGGTGGAA
    CAGAAACAGA GGTTACATAA ATGTGCATAT TCCATAAGAG ATTCAAAATT GAGAGATCTC
    TTAATACACG GTGTTGCTTA TCATCATGCT GGCATGGAGC TGTCAGATAG AAAAGTAGTC
    GAAGGAGCTT TTACTGTTGG AGATCTACCA GTTCTTTTTA CTACTAGTAC CTTAGCTATG
    GGAGTAAATT TGCCTGCTCA TCTAGTAGTT ATCAAATCTA CAATGCATTA TGCTGGAGGG
    ATGTTTGAAG AGTACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCAAC TGCAGTTATT ATGACTCGGT TAAGTACAAA AGAGAAGTAT
    ATTCAGATGC TGGCTTGTAG TGACACTGTA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT GTTGCATACC ATCACAGATG TCAACATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTGTA TATCAGAGCC TTGAAGAATC CGTCTCACTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATTTG
    AATGATTTAT CATCTCTGGA CTTGATAAAG ATGGATGAAG ATATTAATTT CAAGCCAACT
    GAGGCAGGAA GATTGATGGC TTGGTACTAT GTTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATCAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG GATAAATGAA AAGAAAACAC TGAATACTTT GAACAAAGAT
    CCAAATCGGA TAACTATCAG ATTTCCAATG GAAGGAAAAA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCA TACAAGACTT TGCTTTGACA
    CAAGATACCT CAAAGATTTT CAGGAATGGC TCACGAATTA CGAGATGGTT ATCAGATTTT
    GTGGCTGTTC AAGAAAAGTT TGCTGTGCTT TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCTGCAT GTATCCAAAC AACTGGAAAA AATCGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AGCTTGAACT GATTTTAAAT AGACATCCCC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTGTCTGCC AAAATACGAA CTTGAAGTGG AACAGATTGC AAGATACAGT
    GATACCACGG CAGAAGTTTT AGTGACTGTT ATATTAAGAA ACTTCGAACC ACTGCAAACT
    AAAAGAACAG CACCAGATTC TCACTATGTT ACCTTAATCA TAGGCGATGC AGATAATCAG
    CTGATTTTTA AGCATAAAAT TATGGATTCT GTTTTGCTGA AATCTGGAAA TTGGGCTAAA
    AAGATCGATG TGAAGAGAGC TCTGAAATCT GAAGATCTTA GCATAAATCT CATTAGTTCT
    GAGTATGTTG GACTTGATAT TCAGCAGAAA TTTACAGTCT TTTACTTAGG ACCCAAGAGA
    TTTGGAAATC AAATAATTAT GCAAAAAAAA TCAGAAACAG AGATCTTCCA TTCTAAACGT
    TCAGATGGAT CTCCTGTAGC AGGTCCCATT AAAGGAATGA CTGCCTGCAG AAAGCCTGGG
    CACCGAGAGT GTAACCATCA CTGTAAAAAG AAACACACGT GTGGACATGA CTGCTGTAAA
    ACTGGAGTTG CCCAGAAGCC AGAAATGAAA GAGTCAACAA TTTCTTCTTA TTTATCTGAT
    TTAAGAAACA GGAATGCAGT TTCATCTCTT CCTCCAGTTA AACGTCTAAA GATGCAGATG
    AATAAATCTC AAAGTGTGGA CCTTAAAGAG TTTGGTTTTA CTCCACAACC TTCTCTTCCC
    AGTCTGTCAA GGTCAGAATG TTTAAACATA CCTAAATTGC CTGTAATGGA GCAGAGGGGT
    CAGCACGAGC TCTATGCAGA AGGTCAACAA CAAGAACCAC CGGGATATCA GGACAAAGGT
    TTTTCCAGAC AGACCTCTTC TACAACCTCA AATGAGAAAA CCCCTCAAAC AACACTGTTC
    AACAGAAATA TTAAGGCAAC TGGAAACCAG AATTTGACAG CTGTGGTTAA AAGTAATTCT
    TCCTATTCAG AAGTTTTGAA TGTGAACTTT GAATTGGGAA ATGAAGTTTG GGATGATTTT
    GATGATGAAA ACTTAATAGA AATTGCTAGC TTTTCAGCTG ATACTGAAAA GACAAAAGCA
    GGATTTGGAA ACACTTTGAG TTCAAGTGCC AGGGAAAATA AGCTACCCTT CCAAGAATCA
    AAAAGCAAAT TCCAAAGAAA AATGTCAAAC AGTTTTGTTT CATCACATGA GAAGCCAGAT
    ATTTATTTAC CAAATTCTGC TCTGTCCAAG ATCAGTGCAT CCTCCATGAC AAAATTACCT
    CAACCAGCTG GAAATGTCCC TTTCCAAGAA ATAAAACAAC AGAATCTGTC ACCAGAGATT
    GAAAAGCTAT GCTTTGCTCA CTCTGAAAAA ATATCAAGCT TTTCAAATTC TGTTACGCAA
    GTGGATTGCT TTATTAGAAA CAGTGAATAT AAAAAGCAAC CTGACTTCAG TAGGTATCAT
    CTTGATGATG AAGCTGATGA AATGAAGTCT TTTCTGGGAA TATTTGATGG TATTTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013852837.1
    CDS91..4530
    Translation

    Target ORF information:

    RefSeq Version XM_013997383.1
    Organism Sus scrofa(Pig)
    Definition Sus scrofa HFM1, ATP-dependent DNA helicase homolog (HFM1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_013997383.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCTGAGAT CACATGACTC CTTGTGTTCT TTGGATAATT TATTTTTTGA AAAAACAGAT 
    GAAGCTGAAA AATACCCAGA CAATGAAAAA TCATTAGATT GGTTTCTCCC ACCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAAGCA TTAGAGGAAG ACATGCAAAG TCATAAACTG
    TTAGGTCAGA AAAAGAGGTC AAAAATGTTA ACATCCAATT TAAAGATAAC TAATGAAGAC
    ACAAGTTATA CTTCACCAAC ACAAAAATTC CAGTGTGCTT TCCCTTCTAA TAAATACGAA
    GAAGGCGATC TAAATTTAGT AGGGGCAGGT AGCAATGACT TACATGTTGC TGGCAAGCTG
    ACTTATGGTT CTCCTCAGAA GTACAAAAGT CACATTGGCC CTGAGATAGC ACTTGAGAAA
    AATGTTCCAG ATGATGCGAA AGTAGTTAAT TTTGCATACG ATAAAGGAGA GAGCACATCG
    GCCTTCCGAA AAAGATTATT TAAAATATCT GATAGTATAC ATGGGAATGC TTGTTCTAAT
    GACATTGCAA ACTTGGACTC TGATATTGGC TCAGCGAAGA TTGCCCAAAT GGAAATGAGC
    GAAGGGAAAT CGTGGAAATG GAACAATAGT AATCAGAAAC CTCAATATTC AGAAATTAAC
    ATGTTTACAG CCAGTGATAC TTTTTCTGCT TCTAAAATCA GAGAAGGCAT ATTCAAAGCA
    CCATCTTTTT CAGTTGCATC CCAGCCTCAT GAAGTTCAAG GGATAATAGA AAATGGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATCCCG GCAAAATTTA GAAGTGTTTT CAAAGAATTC
    CCATATTTCA ACTATATCCA GTCTAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    GTTGTGGTTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAA TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGCAGTCA GCGTTTTGAT GACTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC GGGAGATACA GTCATGGATG ACCTGTTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCAACTGGT TCGACTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTT GTCAGCAGGA TGAAAACTGT GCAGTTTTTA
    TCTCATGCTG TGGAAAATCC CAGAAATATT ATTCCAATGA GATTTGTAGC TGTATCTGCA
    ACAATTCCAA ATGCTGAGGA TATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCCGCTATC
    TGTCTGAAAA TGGATGAGAA ACATAGACCA GTGAAGCTTC GGAAAGTGGT CCTTGGTTTT
    CCCTGCAGTA ATAACCAAAC TGAATTTAAG TTTGATTTAA CCCTCAACTA CAAAATTGCC
    AGTGTTGTAC AAACGTACTC CGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTACAAC AGGCTGCTTC CATTCTTGTG AAAGATGCCA AGTTTATTAT GACGGTGGAA
    CAGAAACAGA GGTTACATAA ATGTGCATAT TCCATAAGAG ATTCAAAATT GAGAGATCTC
    TTAATACACG GTGTTGCTTA TCATCATGCT GGCATGGAGC TGTCAGATAG AAAAGTAGTC
    GAAGGAGCTT TTACTGTTGG AGATCTACCA GTTCTTTTTA CTACTAGTAC CTTAGCTATG
    GGAGTAAATT TGCCTGCTCA TCTAGTAGTT ATCAAATCTA CAATGCATTA TGCTGGAGGG
    ATGTTTGAAG AGTACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCAAC TGCAGTTATT ATGACTCGGT TAAGTACAAA AGAGAAGTAT
    ATTCAGATGC TGGCTTGTAG TGACACTGTA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT GTTGCATACC ATCACAGATG TCAACATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTGTA TATCAGAGCC TTGAAGAATC CGTCTCACTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATTTG
    AATGATTTAT CATCTCTGGA CTTGATAAAG ATGGATGAAG ATATTAATTT CAAGCCAACT
    GAGGCAGGAA GATTGATGGC TTGGTACTAT GTTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATCAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG GATAAATGAA AAGAAAACAC TGAATACTTT GAACAAAGAT
    CCAAATCGGA TAACTATCAG ATTTCCAATG GAAGGAAAAA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCA TACAAGACTT TGCTTTGACA
    CAAGATACCT CAAAGATTTT CAGGAATGGC TCACGAATTA CGAGATGGTT ATCAGATTTT
    GTGGCTGTTC AAGAAAAGTT TGCTGTGCTT TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCTGCAT GTATCCAAAC AACTGGAAAA AATCGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AGCTTGAACT GATTTTAAAT AGACATCCCC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTGTCTGCC AAAATACGAA CTTGAAGTGG AACAGATTGC AAGATACAGT
    GATACCACGG CAGAAGTTTT AGTGACTGTT ATATTAAGAA ACTTCGAACC ACTGCAAACT
    AAAAGAACAG CACCAGATTC TCACTATGTT ACCTTAATCA TAGGCGATGC AGATAATCAG
    CTGATTTTTA AGCATAAAAT TATGGATTCT GTTTTGCTGA AATCTGGAAA TTGGGCTAAA
    AAGATCGATG TGAAGAGAGC TCTGAAATCT GAAGATCTTA GCATAAATCT CATTAGTTCT
    GAGTATGTTG GACTTGATAT TCAGCAGAAA TTTACAGTCT TTTACTTAGG ACCCAAGAGA
    TTTGGAAATC AAATAATTAT GCAAAAAAAA TCAGAAACAG AGATCTTCCA TTCTAAACGT
    TCAGATGGAT CTCCTGTAGC AGGTCCCATT AAAGGAATGA CTGCCTGCAG AAAGCCTGGG
    CACCGAGAGT GTAACCATCA CTGTAAAAAG AAACACACGT GTGGACATGA CTGCTGTAAA
    ACTGGAGTTG CCCAGAAGCC AGAAATGAAA GAGTCAACAA TTTCTTCTTA TTTATCTGAT
    TTAAGAAACA GGAATGCAGT TTCATCTCTT CCTCCAGTTA AACGTCTAAA GATGCAGATG
    AATAAATCTC AAAGTGTGGA CCTTAAAGAG TTTGGTTTTA CTCCACAACC TTCTCTTCCC
    AGTCTGTCAA GGTCAGAATG TTTAAACATA CCTAAATTGC CTGTAATGGA GCAGAGGGGT
    CAGCACGAGC TCTATGCAGA AGGTCAACAA CAAGAACCAC CGGGATATCA GGACAAAGGT
    TTTTCCAGAC AGACCTCTTC TACAACCTCA AATGAGAAAA CCCCTCAAAC AACACTGTTC
    AACAGAAATA TTAAGGCAAC TGGAAACCAG AATTTGACAG CTGTGGTTAA AAGTAATTCT
    TCCTATTCAG AAGTTTTGAA TGTGAACTTT GAATTGGGAA ATGAAGTTTG GGATGATTTT
    GATGATGAAA ACTTAATAGA AATTGCTAGC TTTTCAGCTG ATACTGAAAA GACAAAAGCA
    GGATTTGGAA ACACTTTGAG TTCAAGTGCC AGGGAAAATA AGCTACCCTT CCAAGAATCA
    AAAAGCAAAT TCCAAAGAAA AATGTCAAAC AGTTTTGTTT CATCACATGA GAAGCCAGAT
    ATTTATTTAC CAAATTCTGC TCTGTCCAAG ATCAGTGCAT CCTCCATGAC AAAATTACCT
    CAACCAGCTG GAAATGTCCC TTTCCAAGAA ATAAAACAAC AGAATCTGTC ACCAGAGATT
    GAAAAGCTAT GCTTTGCTCA CTCTGAAAAA ATATCAAGCT TTTCAAATTC TGTTACGCAA
    GTGGATTGCT TTATTAGAAA CAGTGAATAT AAAAAGCAAC CTGACTTCAG TAGGTATCAT
    CTTGATGATG AAGCTGATGA AATGAAGTCT TTTCTGGGAA TATTTGATGG TATTTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe35333
    Clone ID Related Accession (Same CDS sequence) XM_013997383.1 , XM_013997384.1 , XM_013997385.1
    Accession Version XM_013997384.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4440bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-10
    Organism Sus scrofa(Pig)
    Product probable ATP-dependent DNA helicase HFM1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003610184.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCTGAGAT CACATGACTC CTTGTGTTCT TTGGATAATT TATTTTTTGA AAAAACAGAT 
    GAAGCTGAAA AATACCCAGA CAATGAAAAA TCATTAGATT GGTTTCTCCC ACCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAAGCA TTAGAGGAAG ACATGCAAAG TCATAAACTG
    TTAGGTCAGA AAAAGAGGTC AAAAATGTTA ACATCCAATT TAAAGATAAC TAATGAAGAC
    ACAAGTTATA CTTCACCAAC ACAAAAATTC CAGTGTGCTT TCCCTTCTAA TAAATACGAA
    GAAGGCGATC TAAATTTAGT AGGGGCAGGT AGCAATGACT TACATGTTGC TGGCAAGCTG
    ACTTATGGTT CTCCTCAGAA GTACAAAAGT CACATTGGCC CTGAGATAGC ACTTGAGAAA
    AATGTTCCAG ATGATGCGAA AGTAGTTAAT TTTGCATACG ATAAAGGAGA GAGCACATCG
    GCCTTCCGAA AAAGATTATT TAAAATATCT GATAGTATAC ATGGGAATGC TTGTTCTAAT
    GACATTGCAA ACTTGGACTC TGATATTGGC TCAGCGAAGA TTGCCCAAAT GGAAATGAGC
    GAAGGGAAAT CGTGGAAATG GAACAATAGT AATCAGAAAC CTCAATATTC AGAAATTAAC
    ATGTTTACAG CCAGTGATAC TTTTTCTGCT TCTAAAATCA GAGAAGGCAT ATTCAAAGCA
    CCATCTTTTT CAGTTGCATC CCAGCCTCAT GAAGTTCAAG GGATAATAGA AAATGGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATCCCG GCAAAATTTA GAAGTGTTTT CAAAGAATTC
    CCATATTTCA ACTATATCCA GTCTAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    GTTGTGGTTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAA TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGCAGTCA GCGTTTTGAT GACTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC GGGAGATACA GTCATGGATG ACCTGTTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCAACTGGT TCGACTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTT GTCAGCAGGA TGAAAACTGT GCAGTTTTTA
    TCTCATGCTG TGGAAAATCC CAGAAATATT ATTCCAATGA GATTTGTAGC TGTATCTGCA
    ACAATTCCAA ATGCTGAGGA TATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCCGCTATC
    TGTCTGAAAA TGGATGAGAA ACATAGACCA GTGAAGCTTC GGAAAGTGGT CCTTGGTTTT
    CCCTGCAGTA ATAACCAAAC TGAATTTAAG TTTGATTTAA CCCTCAACTA CAAAATTGCC
    AGTGTTGTAC AAACGTACTC CGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTACAAC AGGCTGCTTC CATTCTTGTG AAAGATGCCA AGTTTATTAT GACGGTGGAA
    CAGAAACAGA GGTTACATAA ATGTGCATAT TCCATAAGAG ATTCAAAATT GAGAGATCTC
    TTAATACACG GTGTTGCTTA TCATCATGCT GGCATGGAGC TGTCAGATAG AAAAGTAGTC
    GAAGGAGCTT TTACTGTTGG AGATCTACCA GTTCTTTTTA CTACTAGTAC CTTAGCTATG
    GGAGTAAATT TGCCTGCTCA TCTAGTAGTT ATCAAATCTA CAATGCATTA TGCTGGAGGG
    ATGTTTGAAG AGTACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCAAC TGCAGTTATT ATGACTCGGT TAAGTACAAA AGAGAAGTAT
    ATTCAGATGC TGGCTTGTAG TGACACTGTA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT GTTGCATACC ATCACAGATG TCAACATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTGTA TATCAGAGCC TTGAAGAATC CGTCTCACTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATTTG
    AATGATTTAT CATCTCTGGA CTTGATAAAG ATGGATGAAG ATATTAATTT CAAGCCAACT
    GAGGCAGGAA GATTGATGGC TTGGTACTAT GTTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATCAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG GATAAATGAA AAGAAAACAC TGAATACTTT GAACAAAGAT
    CCAAATCGGA TAACTATCAG ATTTCCAATG GAAGGAAAAA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCA TACAAGACTT TGCTTTGACA
    CAAGATACCT CAAAGATTTT CAGGAATGGC TCACGAATTA CGAGATGGTT ATCAGATTTT
    GTGGCTGTTC AAGAAAAGTT TGCTGTGCTT TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCTGCAT GTATCCAAAC AACTGGAAAA AATCGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AGCTTGAACT GATTTTAAAT AGACATCCCC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTGTCTGCC AAAATACGAA CTTGAAGTGG AACAGATTGC AAGATACAGT
    GATACCACGG CAGAAGTTTT AGTGACTGTT ATATTAAGAA ACTTCGAACC ACTGCAAACT
    AAAAGAACAG CACCAGATTC TCACTATGTT ACCTTAATCA TAGGCGATGC AGATAATCAG
    CTGATTTTTA AGCATAAAAT TATGGATTCT GTTTTGCTGA AATCTGGAAA TTGGGCTAAA
    AAGATCGATG TGAAGAGAGC TCTGAAATCT GAAGATCTTA GCATAAATCT CATTAGTTCT
    GAGTATGTTG GACTTGATAT TCAGCAGAAA TTTACAGTCT TTTACTTAGG ACCCAAGAGA
    TTTGGAAATC AAATAATTAT GCAAAAAAAA TCAGAAACAG AGATCTTCCA TTCTAAACGT
    TCAGATGGAT CTCCTGTAGC AGGTCCCATT AAAGGAATGA CTGCCTGCAG AAAGCCTGGG
    CACCGAGAGT GTAACCATCA CTGTAAAAAG AAACACACGT GTGGACATGA CTGCTGTAAA
    ACTGGAGTTG CCCAGAAGCC AGAAATGAAA GAGTCAACAA TTTCTTCTTA TTTATCTGAT
    TTAAGAAACA GGAATGCAGT TTCATCTCTT CCTCCAGTTA AACGTCTAAA GATGCAGATG
    AATAAATCTC AAAGTGTGGA CCTTAAAGAG TTTGGTTTTA CTCCACAACC TTCTCTTCCC
    AGTCTGTCAA GGTCAGAATG TTTAAACATA CCTAAATTGC CTGTAATGGA GCAGAGGGGT
    CAGCACGAGC TCTATGCAGA AGGTCAACAA CAAGAACCAC CGGGATATCA GGACAAAGGT
    TTTTCCAGAC AGACCTCTTC TACAACCTCA AATGAGAAAA CCCCTCAAAC AACACTGTTC
    AACAGAAATA TTAAGGCAAC TGGAAACCAG AATTTGACAG CTGTGGTTAA AAGTAATTCT
    TCCTATTCAG AAGTTTTGAA TGTGAACTTT GAATTGGGAA ATGAAGTTTG GGATGATTTT
    GATGATGAAA ACTTAATAGA AATTGCTAGC TTTTCAGCTG ATACTGAAAA GACAAAAGCA
    GGATTTGGAA ACACTTTGAG TTCAAGTGCC AGGGAAAATA AGCTACCCTT CCAAGAATCA
    AAAAGCAAAT TCCAAAGAAA AATGTCAAAC AGTTTTGTTT CATCACATGA GAAGCCAGAT
    ATTTATTTAC CAAATTCTGC TCTGTCCAAG ATCAGTGCAT CCTCCATGAC AAAATTACCT
    CAACCAGCTG GAAATGTCCC TTTCCAAGAA ATAAAACAAC AGAATCTGTC ACCAGAGATT
    GAAAAGCTAT GCTTTGCTCA CTCTGAAAAA ATATCAAGCT TTTCAAATTC TGTTACGCAA
    GTGGATTGCT TTATTAGAAA CAGTGAATAT AAAAAGCAAC CTGACTTCAG TAGGTATCAT
    CTTGATGATG AAGCTGATGA AATGAAGTCT TTTCTGGGAA TATTTGATGG TATTTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013852838.1
    CDS406..4845
    Translation

    Target ORF information:

    RefSeq Version XM_013997384.1
    Organism Sus scrofa(Pig)
    Definition Sus scrofa HFM1, ATP-dependent DNA helicase homolog (HFM1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_013997384.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCTGAGAT CACATGACTC CTTGTGTTCT TTGGATAATT TATTTTTTGA AAAAACAGAT 
    GAAGCTGAAA AATACCCAGA CAATGAAAAA TCATTAGATT GGTTTCTCCC ACCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAAGCA TTAGAGGAAG ACATGCAAAG TCATAAACTG
    TTAGGTCAGA AAAAGAGGTC AAAAATGTTA ACATCCAATT TAAAGATAAC TAATGAAGAC
    ACAAGTTATA CTTCACCAAC ACAAAAATTC CAGTGTGCTT TCCCTTCTAA TAAATACGAA
    GAAGGCGATC TAAATTTAGT AGGGGCAGGT AGCAATGACT TACATGTTGC TGGCAAGCTG
    ACTTATGGTT CTCCTCAGAA GTACAAAAGT CACATTGGCC CTGAGATAGC ACTTGAGAAA
    AATGTTCCAG ATGATGCGAA AGTAGTTAAT TTTGCATACG ATAAAGGAGA GAGCACATCG
    GCCTTCCGAA AAAGATTATT TAAAATATCT GATAGTATAC ATGGGAATGC TTGTTCTAAT
    GACATTGCAA ACTTGGACTC TGATATTGGC TCAGCGAAGA TTGCCCAAAT GGAAATGAGC
    GAAGGGAAAT CGTGGAAATG GAACAATAGT AATCAGAAAC CTCAATATTC AGAAATTAAC
    ATGTTTACAG CCAGTGATAC TTTTTCTGCT TCTAAAATCA GAGAAGGCAT ATTCAAAGCA
    CCATCTTTTT CAGTTGCATC CCAGCCTCAT GAAGTTCAAG GGATAATAGA AAATGGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATCCCG GCAAAATTTA GAAGTGTTTT CAAAGAATTC
    CCATATTTCA ACTATATCCA GTCTAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    GTTGTGGTTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAA TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGCAGTCA GCGTTTTGAT GACTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC GGGAGATACA GTCATGGATG ACCTGTTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCAACTGGT TCGACTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTT GTCAGCAGGA TGAAAACTGT GCAGTTTTTA
    TCTCATGCTG TGGAAAATCC CAGAAATATT ATTCCAATGA GATTTGTAGC TGTATCTGCA
    ACAATTCCAA ATGCTGAGGA TATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCCGCTATC
    TGTCTGAAAA TGGATGAGAA ACATAGACCA GTGAAGCTTC GGAAAGTGGT CCTTGGTTTT
    CCCTGCAGTA ATAACCAAAC TGAATTTAAG TTTGATTTAA CCCTCAACTA CAAAATTGCC
    AGTGTTGTAC AAACGTACTC CGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTACAAC AGGCTGCTTC CATTCTTGTG AAAGATGCCA AGTTTATTAT GACGGTGGAA
    CAGAAACAGA GGTTACATAA ATGTGCATAT TCCATAAGAG ATTCAAAATT GAGAGATCTC
    TTAATACACG GTGTTGCTTA TCATCATGCT GGCATGGAGC TGTCAGATAG AAAAGTAGTC
    GAAGGAGCTT TTACTGTTGG AGATCTACCA GTTCTTTTTA CTACTAGTAC CTTAGCTATG
    GGAGTAAATT TGCCTGCTCA TCTAGTAGTT ATCAAATCTA CAATGCATTA TGCTGGAGGG
    ATGTTTGAAG AGTACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCAAC TGCAGTTATT ATGACTCGGT TAAGTACAAA AGAGAAGTAT
    ATTCAGATGC TGGCTTGTAG TGACACTGTA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT GTTGCATACC ATCACAGATG TCAACATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTGTA TATCAGAGCC TTGAAGAATC CGTCTCACTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATTTG
    AATGATTTAT CATCTCTGGA CTTGATAAAG ATGGATGAAG ATATTAATTT CAAGCCAACT
    GAGGCAGGAA GATTGATGGC TTGGTACTAT GTTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATCAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG GATAAATGAA AAGAAAACAC TGAATACTTT GAACAAAGAT
    CCAAATCGGA TAACTATCAG ATTTCCAATG GAAGGAAAAA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCA TACAAGACTT TGCTTTGACA
    CAAGATACCT CAAAGATTTT CAGGAATGGC TCACGAATTA CGAGATGGTT ATCAGATTTT
    GTGGCTGTTC AAGAAAAGTT TGCTGTGCTT TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCTGCAT GTATCCAAAC AACTGGAAAA AATCGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AGCTTGAACT GATTTTAAAT AGACATCCCC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTGTCTGCC AAAATACGAA CTTGAAGTGG AACAGATTGC AAGATACAGT
    GATACCACGG CAGAAGTTTT AGTGACTGTT ATATTAAGAA ACTTCGAACC ACTGCAAACT
    AAAAGAACAG CACCAGATTC TCACTATGTT ACCTTAATCA TAGGCGATGC AGATAATCAG
    CTGATTTTTA AGCATAAAAT TATGGATTCT GTTTTGCTGA AATCTGGAAA TTGGGCTAAA
    AAGATCGATG TGAAGAGAGC TCTGAAATCT GAAGATCTTA GCATAAATCT CATTAGTTCT
    GAGTATGTTG GACTTGATAT TCAGCAGAAA TTTACAGTCT TTTACTTAGG ACCCAAGAGA
    TTTGGAAATC AAATAATTAT GCAAAAAAAA TCAGAAACAG AGATCTTCCA TTCTAAACGT
    TCAGATGGAT CTCCTGTAGC AGGTCCCATT AAAGGAATGA CTGCCTGCAG AAAGCCTGGG
    CACCGAGAGT GTAACCATCA CTGTAAAAAG AAACACACGT GTGGACATGA CTGCTGTAAA
    ACTGGAGTTG CCCAGAAGCC AGAAATGAAA GAGTCAACAA TTTCTTCTTA TTTATCTGAT
    TTAAGAAACA GGAATGCAGT TTCATCTCTT CCTCCAGTTA AACGTCTAAA GATGCAGATG
    AATAAATCTC AAAGTGTGGA CCTTAAAGAG TTTGGTTTTA CTCCACAACC TTCTCTTCCC
    AGTCTGTCAA GGTCAGAATG TTTAAACATA CCTAAATTGC CTGTAATGGA GCAGAGGGGT
    CAGCACGAGC TCTATGCAGA AGGTCAACAA CAAGAACCAC CGGGATATCA GGACAAAGGT
    TTTTCCAGAC AGACCTCTTC TACAACCTCA AATGAGAAAA CCCCTCAAAC AACACTGTTC
    AACAGAAATA TTAAGGCAAC TGGAAACCAG AATTTGACAG CTGTGGTTAA AAGTAATTCT
    TCCTATTCAG AAGTTTTGAA TGTGAACTTT GAATTGGGAA ATGAAGTTTG GGATGATTTT
    GATGATGAAA ACTTAATAGA AATTGCTAGC TTTTCAGCTG ATACTGAAAA GACAAAAGCA
    GGATTTGGAA ACACTTTGAG TTCAAGTGCC AGGGAAAATA AGCTACCCTT CCAAGAATCA
    AAAAGCAAAT TCCAAAGAAA AATGTCAAAC AGTTTTGTTT CATCACATGA GAAGCCAGAT
    ATTTATTTAC CAAATTCTGC TCTGTCCAAG ATCAGTGCAT CCTCCATGAC AAAATTACCT
    CAACCAGCTG GAAATGTCCC TTTCCAAGAA ATAAAACAAC AGAATCTGTC ACCAGAGATT
    GAAAAGCTAT GCTTTGCTCA CTCTGAAAAA ATATCAAGCT TTTCAAATTC TGTTACGCAA
    GTGGATTGCT TTATTAGAAA CAGTGAATAT AAAAAGCAAC CTGACTTCAG TAGGTATCAT
    CTTGATGATG AAGCTGATGA AATGAAGTCT TTTCTGGGAA TATTTGATGG TATTTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe35333
    Clone ID Related Accession (Same CDS sequence) XM_013997383.1 , XM_013997384.1 , XM_013997385.1
    Accession Version XM_013997385.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4440bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-10
    Organism Sus scrofa(Pig)
    Product probable ATP-dependent DNA helicase HFM1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003610184.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCTGAGAT CACATGACTC CTTGTGTTCT TTGGATAATT TATTTTTTGA AAAAACAGAT 
    GAAGCTGAAA AATACCCAGA CAATGAAAAA TCATTAGATT GGTTTCTCCC ACCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAAGCA TTAGAGGAAG ACATGCAAAG TCATAAACTG
    TTAGGTCAGA AAAAGAGGTC AAAAATGTTA ACATCCAATT TAAAGATAAC TAATGAAGAC
    ACAAGTTATA CTTCACCAAC ACAAAAATTC CAGTGTGCTT TCCCTTCTAA TAAATACGAA
    GAAGGCGATC TAAATTTAGT AGGGGCAGGT AGCAATGACT TACATGTTGC TGGCAAGCTG
    ACTTATGGTT CTCCTCAGAA GTACAAAAGT CACATTGGCC CTGAGATAGC ACTTGAGAAA
    AATGTTCCAG ATGATGCGAA AGTAGTTAAT TTTGCATACG ATAAAGGAGA GAGCACATCG
    GCCTTCCGAA AAAGATTATT TAAAATATCT GATAGTATAC ATGGGAATGC TTGTTCTAAT
    GACATTGCAA ACTTGGACTC TGATATTGGC TCAGCGAAGA TTGCCCAAAT GGAAATGAGC
    GAAGGGAAAT CGTGGAAATG GAACAATAGT AATCAGAAAC CTCAATATTC AGAAATTAAC
    ATGTTTACAG CCAGTGATAC TTTTTCTGCT TCTAAAATCA GAGAAGGCAT ATTCAAAGCA
    CCATCTTTTT CAGTTGCATC CCAGCCTCAT GAAGTTCAAG GGATAATAGA AAATGGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATCCCG GCAAAATTTA GAAGTGTTTT CAAAGAATTC
    CCATATTTCA ACTATATCCA GTCTAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    GTTGTGGTTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAA TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGCAGTCA GCGTTTTGAT GACTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC GGGAGATACA GTCATGGATG ACCTGTTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCAACTGGT TCGACTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTT GTCAGCAGGA TGAAAACTGT GCAGTTTTTA
    TCTCATGCTG TGGAAAATCC CAGAAATATT ATTCCAATGA GATTTGTAGC TGTATCTGCA
    ACAATTCCAA ATGCTGAGGA TATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCCGCTATC
    TGTCTGAAAA TGGATGAGAA ACATAGACCA GTGAAGCTTC GGAAAGTGGT CCTTGGTTTT
    CCCTGCAGTA ATAACCAAAC TGAATTTAAG TTTGATTTAA CCCTCAACTA CAAAATTGCC
    AGTGTTGTAC AAACGTACTC CGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTACAAC AGGCTGCTTC CATTCTTGTG AAAGATGCCA AGTTTATTAT GACGGTGGAA
    CAGAAACAGA GGTTACATAA ATGTGCATAT TCCATAAGAG ATTCAAAATT GAGAGATCTC
    TTAATACACG GTGTTGCTTA TCATCATGCT GGCATGGAGC TGTCAGATAG AAAAGTAGTC
    GAAGGAGCTT TTACTGTTGG AGATCTACCA GTTCTTTTTA CTACTAGTAC CTTAGCTATG
    GGAGTAAATT TGCCTGCTCA TCTAGTAGTT ATCAAATCTA CAATGCATTA TGCTGGAGGG
    ATGTTTGAAG AGTACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCAAC TGCAGTTATT ATGACTCGGT TAAGTACAAA AGAGAAGTAT
    ATTCAGATGC TGGCTTGTAG TGACACTGTA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT GTTGCATACC ATCACAGATG TCAACATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTGTA TATCAGAGCC TTGAAGAATC CGTCTCACTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATTTG
    AATGATTTAT CATCTCTGGA CTTGATAAAG ATGGATGAAG ATATTAATTT CAAGCCAACT
    GAGGCAGGAA GATTGATGGC TTGGTACTAT GTTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATCAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG GATAAATGAA AAGAAAACAC TGAATACTTT GAACAAAGAT
    CCAAATCGGA TAACTATCAG ATTTCCAATG GAAGGAAAAA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCA TACAAGACTT TGCTTTGACA
    CAAGATACCT CAAAGATTTT CAGGAATGGC TCACGAATTA CGAGATGGTT ATCAGATTTT
    GTGGCTGTTC AAGAAAAGTT TGCTGTGCTT TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCTGCAT GTATCCAAAC AACTGGAAAA AATCGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AGCTTGAACT GATTTTAAAT AGACATCCCC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTGTCTGCC AAAATACGAA CTTGAAGTGG AACAGATTGC AAGATACAGT
    GATACCACGG CAGAAGTTTT AGTGACTGTT ATATTAAGAA ACTTCGAACC ACTGCAAACT
    AAAAGAACAG CACCAGATTC TCACTATGTT ACCTTAATCA TAGGCGATGC AGATAATCAG
    CTGATTTTTA AGCATAAAAT TATGGATTCT GTTTTGCTGA AATCTGGAAA TTGGGCTAAA
    AAGATCGATG TGAAGAGAGC TCTGAAATCT GAAGATCTTA GCATAAATCT CATTAGTTCT
    GAGTATGTTG GACTTGATAT TCAGCAGAAA TTTACAGTCT TTTACTTAGG ACCCAAGAGA
    TTTGGAAATC AAATAATTAT GCAAAAAAAA TCAGAAACAG AGATCTTCCA TTCTAAACGT
    TCAGATGGAT CTCCTGTAGC AGGTCCCATT AAAGGAATGA CTGCCTGCAG AAAGCCTGGG
    CACCGAGAGT GTAACCATCA CTGTAAAAAG AAACACACGT GTGGACATGA CTGCTGTAAA
    ACTGGAGTTG CCCAGAAGCC AGAAATGAAA GAGTCAACAA TTTCTTCTTA TTTATCTGAT
    TTAAGAAACA GGAATGCAGT TTCATCTCTT CCTCCAGTTA AACGTCTAAA GATGCAGATG
    AATAAATCTC AAAGTGTGGA CCTTAAAGAG TTTGGTTTTA CTCCACAACC TTCTCTTCCC
    AGTCTGTCAA GGTCAGAATG TTTAAACATA CCTAAATTGC CTGTAATGGA GCAGAGGGGT
    CAGCACGAGC TCTATGCAGA AGGTCAACAA CAAGAACCAC CGGGATATCA GGACAAAGGT
    TTTTCCAGAC AGACCTCTTC TACAACCTCA AATGAGAAAA CCCCTCAAAC AACACTGTTC
    AACAGAAATA TTAAGGCAAC TGGAAACCAG AATTTGACAG CTGTGGTTAA AAGTAATTCT
    TCCTATTCAG AAGTTTTGAA TGTGAACTTT GAATTGGGAA ATGAAGTTTG GGATGATTTT
    GATGATGAAA ACTTAATAGA AATTGCTAGC TTTTCAGCTG ATACTGAAAA GACAAAAGCA
    GGATTTGGAA ACACTTTGAG TTCAAGTGCC AGGGAAAATA AGCTACCCTT CCAAGAATCA
    AAAAGCAAAT TCCAAAGAAA AATGTCAAAC AGTTTTGTTT CATCACATGA GAAGCCAGAT
    ATTTATTTAC CAAATTCTGC TCTGTCCAAG ATCAGTGCAT CCTCCATGAC AAAATTACCT
    CAACCAGCTG GAAATGTCCC TTTCCAAGAA ATAAAACAAC AGAATCTGTC ACCAGAGATT
    GAAAAGCTAT GCTTTGCTCA CTCTGAAAAA ATATCAAGCT TTTCAAATTC TGTTACGCAA
    GTGGATTGCT TTATTAGAAA CAGTGAATAT AAAAAGCAAC CTGACTTCAG TAGGTATCAT
    CTTGATGATG AAGCTGATGA AATGAAGTCT TTTCTGGGAA TATTTGATGG TATTTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013852839.1
    CDS201..4640
    Translation

    Target ORF information:

    RefSeq Version XM_013997385.1
    Organism Sus scrofa(Pig)
    Definition Sus scrofa HFM1, ATP-dependent DNA helicase homolog (HFM1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_013997385.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCTGAGAT CACATGACTC CTTGTGTTCT TTGGATAATT TATTTTTTGA AAAAACAGAT 
    GAAGCTGAAA AATACCCAGA CAATGAAAAA TCATTAGATT GGTTTCTCCC ACCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAAGCA TTAGAGGAAG ACATGCAAAG TCATAAACTG
    TTAGGTCAGA AAAAGAGGTC AAAAATGTTA ACATCCAATT TAAAGATAAC TAATGAAGAC
    ACAAGTTATA CTTCACCAAC ACAAAAATTC CAGTGTGCTT TCCCTTCTAA TAAATACGAA
    GAAGGCGATC TAAATTTAGT AGGGGCAGGT AGCAATGACT TACATGTTGC TGGCAAGCTG
    ACTTATGGTT CTCCTCAGAA GTACAAAAGT CACATTGGCC CTGAGATAGC ACTTGAGAAA
    AATGTTCCAG ATGATGCGAA AGTAGTTAAT TTTGCATACG ATAAAGGAGA GAGCACATCG
    GCCTTCCGAA AAAGATTATT TAAAATATCT GATAGTATAC ATGGGAATGC TTGTTCTAAT
    GACATTGCAA ACTTGGACTC TGATATTGGC TCAGCGAAGA TTGCCCAAAT GGAAATGAGC
    GAAGGGAAAT CGTGGAAATG GAACAATAGT AATCAGAAAC CTCAATATTC AGAAATTAAC
    ATGTTTACAG CCAGTGATAC TTTTTCTGCT TCTAAAATCA GAGAAGGCAT ATTCAAAGCA
    CCATCTTTTT CAGTTGCATC CCAGCCTCAT GAAGTTCAAG GGATAATAGA AAATGGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATCCCG GCAAAATTTA GAAGTGTTTT CAAAGAATTC
    CCATATTTCA ACTATATCCA GTCTAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    GTTGTGGTTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAA TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGCAGTCA GCGTTTTGAT GACTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC GGGAGATACA GTCATGGATG ACCTGTTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCAACTGGT TCGACTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTT GTCAGCAGGA TGAAAACTGT GCAGTTTTTA
    TCTCATGCTG TGGAAAATCC CAGAAATATT ATTCCAATGA GATTTGTAGC TGTATCTGCA
    ACAATTCCAA ATGCTGAGGA TATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCCGCTATC
    TGTCTGAAAA TGGATGAGAA ACATAGACCA GTGAAGCTTC GGAAAGTGGT CCTTGGTTTT
    CCCTGCAGTA ATAACCAAAC TGAATTTAAG TTTGATTTAA CCCTCAACTA CAAAATTGCC
    AGTGTTGTAC AAACGTACTC CGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTACAAC AGGCTGCTTC CATTCTTGTG AAAGATGCCA AGTTTATTAT GACGGTGGAA
    CAGAAACAGA GGTTACATAA ATGTGCATAT TCCATAAGAG ATTCAAAATT GAGAGATCTC
    TTAATACACG GTGTTGCTTA TCATCATGCT GGCATGGAGC TGTCAGATAG AAAAGTAGTC
    GAAGGAGCTT TTACTGTTGG AGATCTACCA GTTCTTTTTA CTACTAGTAC CTTAGCTATG
    GGAGTAAATT TGCCTGCTCA TCTAGTAGTT ATCAAATCTA CAATGCATTA TGCTGGAGGG
    ATGTTTGAAG AGTACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCAAC TGCAGTTATT ATGACTCGGT TAAGTACAAA AGAGAAGTAT
    ATTCAGATGC TGGCTTGTAG TGACACTGTA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT GTTGCATACC ATCACAGATG TCAACATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTGTA TATCAGAGCC TTGAAGAATC CGTCTCACTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATTTG
    AATGATTTAT CATCTCTGGA CTTGATAAAG ATGGATGAAG ATATTAATTT CAAGCCAACT
    GAGGCAGGAA GATTGATGGC TTGGTACTAT GTTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATCAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG GATAAATGAA AAGAAAACAC TGAATACTTT GAACAAAGAT
    CCAAATCGGA TAACTATCAG ATTTCCAATG GAAGGAAAAA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCA TACAAGACTT TGCTTTGACA
    CAAGATACCT CAAAGATTTT CAGGAATGGC TCACGAATTA CGAGATGGTT ATCAGATTTT
    GTGGCTGTTC AAGAAAAGTT TGCTGTGCTT TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCTGCAT GTATCCAAAC AACTGGAAAA AATCGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AGCTTGAACT GATTTTAAAT AGACATCCCC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTGTCTGCC AAAATACGAA CTTGAAGTGG AACAGATTGC AAGATACAGT
    GATACCACGG CAGAAGTTTT AGTGACTGTT ATATTAAGAA ACTTCGAACC ACTGCAAACT
    AAAAGAACAG CACCAGATTC TCACTATGTT ACCTTAATCA TAGGCGATGC AGATAATCAG
    CTGATTTTTA AGCATAAAAT TATGGATTCT GTTTTGCTGA AATCTGGAAA TTGGGCTAAA
    AAGATCGATG TGAAGAGAGC TCTGAAATCT GAAGATCTTA GCATAAATCT CATTAGTTCT
    GAGTATGTTG GACTTGATAT TCAGCAGAAA TTTACAGTCT TTTACTTAGG ACCCAAGAGA
    TTTGGAAATC AAATAATTAT GCAAAAAAAA TCAGAAACAG AGATCTTCCA TTCTAAACGT
    TCAGATGGAT CTCCTGTAGC AGGTCCCATT AAAGGAATGA CTGCCTGCAG AAAGCCTGGG
    CACCGAGAGT GTAACCATCA CTGTAAAAAG AAACACACGT GTGGACATGA CTGCTGTAAA
    ACTGGAGTTG CCCAGAAGCC AGAAATGAAA GAGTCAACAA TTTCTTCTTA TTTATCTGAT
    TTAAGAAACA GGAATGCAGT TTCATCTCTT CCTCCAGTTA AACGTCTAAA GATGCAGATG
    AATAAATCTC AAAGTGTGGA CCTTAAAGAG TTTGGTTTTA CTCCACAACC TTCTCTTCCC
    AGTCTGTCAA GGTCAGAATG TTTAAACATA CCTAAATTGC CTGTAATGGA GCAGAGGGGT
    CAGCACGAGC TCTATGCAGA AGGTCAACAA CAAGAACCAC CGGGATATCA GGACAAAGGT
    TTTTCCAGAC AGACCTCTTC TACAACCTCA AATGAGAAAA CCCCTCAAAC AACACTGTTC
    AACAGAAATA TTAAGGCAAC TGGAAACCAG AATTTGACAG CTGTGGTTAA AAGTAATTCT
    TCCTATTCAG AAGTTTTGAA TGTGAACTTT GAATTGGGAA ATGAAGTTTG GGATGATTTT
    GATGATGAAA ACTTAATAGA AATTGCTAGC TTTTCAGCTG ATACTGAAAA GACAAAAGCA
    GGATTTGGAA ACACTTTGAG TTCAAGTGCC AGGGAAAATA AGCTACCCTT CCAAGAATCA
    AAAAGCAAAT TCCAAAGAAA AATGTCAAAC AGTTTTGTTT CATCACATGA GAAGCCAGAT
    ATTTATTTAC CAAATTCTGC TCTGTCCAAG ATCAGTGCAT CCTCCATGAC AAAATTACCT
    CAACCAGCTG GAAATGTCCC TTTCCAAGAA ATAAAACAAC AGAATCTGTC ACCAGAGATT
    GAAAAGCTAT GCTTTGCTCA CTCTGAAAAA ATATCAAGCT TTTCAAATTC TGTTACGCAA
    GTGGATTGCT TTATTAGAAA CAGTGAATAT AAAAAGCAAC CTGACTTCAG TAGGTATCAT
    CTTGATGATG AAGCTGATGA AATGAAGTCT TTTCTGGGAA TATTTGATGG TATTTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe35334
    Clone ID Related Accession (Same CDS sequence) XM_013997387.1
    Accession Version XM_013997387.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4314bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-10
    Organism Sus scrofa(Pig)
    Product probable ATP-dependent DNA helicase HFM1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003610184.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGACTCCTT GTGTTCTTTG GATAATTTAT TTTTTGAAAA AACAGATGAA GCTGAAAAGT 
    CAGAAAAAGA GGTCAAAAAT GTTAACATCC AATTTAAAGA TAACTAATGA AGACACAAGT
    TATACTTCAC CAACACAAAA ATTCCAGTGT GCTTTCCCTT CTAATAAATA CGAAGAAGGC
    GATCTAAATT TAGTAGGGGC AGGTAGCAAT GACTTACATG TTGCTGGCAA GCTGACTTAT
    GGTTCTCCTC AGAAGTACAA AAGTCACATT GGCCCTGAGA TAGCACTTGA GAAAAATGTT
    CCAGATGATG CGAAAGTAGT TAATTTTGCA TACGATAAAG GAGAGAGCAC ATCGGCCTTC
    CGAAAAAGAT TATTTAAAAT ATCTGATAGT ATACATGGGA ATGCTTGTTC TAATGACATT
    GCAAACTTGG ACTCTGATAT TGGCTCAGCG AAGATTGCCC AAATGGAAAT GAGCGAAGGG
    AAATCGTGGA AATGGAACAA TAGTAATCAG AAACCTCAAT ATTCAGAAAT TAACATGTTT
    ACAGCCAGTG ATACTTTTTC TGCTTCTAAA ATCAGAGAAG GCATATTCAA AGCACCATCT
    TTTTCAGTTG CATCCCAGCC TCATGAAGTT CAAGGGATAA TAGAAAATGG TTTAGATTCC
    TTGAAGGCTG TCACAGAAAT CCCGGCAAAA TTTAGAAGTG TTTTCAAAGA ATTCCCATAT
    TTCAACTATA TCCAGTCTAA AGCCTTTGAT GATCTTCTTT ATACAGATAG GAATGTTGTG
    GTTTGTGCTC CAACTGGTTC TGGGAAAACT GTAATGTTTG AACTAGCTAT AACAAGATTG
    TTAATGGAAG TACCATTGCC ATGGTCAAAC ATTAAAATTG TTTACATGGC ACCAATAAAA
    GCCCTGTGCA GTCAGCGTTT TGATGACTGG AAAGAAAAAT TTGGACCAAT AGGATTGAAT
    TGTAAAGAAC TCACGGGAGA TACAGTCATG GATGACCTGT TTGAGATTCA GCATGCCCAT
    ATTATTATGA CAACTCCAGA AAAATGGGAT AGCATGACTA GAAAATGGAG AGACAACTCT
    TTGGTCCAAC TGGTTCGACT GTTTCTCATT GATGAGGTAC ATGTTGTAAA AGATGAAAAT
    CGTGGACCAA CTCTTGAAGT TGTTGTCAGC AGGATGAAAA CTGTGCAGTT TTTATCTCAT
    GCTGTGGAAA ATCCCAGAAA TATTATTCCA ATGAGATTTG TAGCTGTATC TGCAACAATT
    CCAAATGCTG AGGATATTGC AGAATGGCTT TCAGATGGTG AAAGATCCGC TATCTGTCTG
    AAAATGGATG AGAAACATAG ACCAGTGAAG CTTCGGAAAG TGGTCCTTGG TTTTCCCTGC
    AGTAATAACC AAACTGAATT TAAGTTTGAT TTAACCCTCA ACTACAAAAT TGCCAGTGTT
    GTACAAACGT ACTCCGATCA GAAACCCACA CTTGTGTTTT GTGCAACAAG GAAAGGTGTA
    CAACAGGCTG CTTCCATTCT TGTGAAAGAT GCCAAGTTTA TTATGACGGT GGAACAGAAA
    CAGAGGTTAC ATAAATGTGC ATATTCCATA AGAGATTCAA AATTGAGAGA TCTCTTAATA
    CACGGTGTTG CTTATCATCA TGCTGGCATG GAGCTGTCAG ATAGAAAAGT AGTCGAAGGA
    GCTTTTACTG TTGGAGATCT ACCAGTTCTT TTTACTACTA GTACCTTAGC TATGGGAGTA
    AATTTGCCTG CTCATCTAGT AGTTATCAAA TCTACAATGC ATTATGCTGG AGGGATGTTT
    GAAGAGTACA GTGAAACAGA TATTCTACAG ATGATTGGTA GAGCTGGTCG ACCTCAATTT
    GACACTACAG CAACTGCAGT TATTATGACT CGGTTAAGTA CAAAAGAGAA GTATATTCAG
    ATGCTGGCTT GTAGTGACAC TGTAGAAAGC AGTTTGCACA GACATCTTAT TGAGCATTTA
    AATGCAGAGA TAGTGTTGCA TACCATCACA GATGTCAACA TTGCTTTGGA ATGGATACGA
    TCAACCTTGC TGTATATCAG AGCCTTGAAG AATCCGTCTC ACTATGGTTT TGCATCTGGA
    TTGAACAAAG ATGGAATTGA ATCAAAATTA CAAGAATTGT GTTTGAAGAA TTTGAATGAT
    TTATCATCTC TGGACTTGAT AAAGATGGAT GAAGATATTA ATTTCAAGCC AACTGAGGCA
    GGAAGATTGA TGGCTTGGTA CTATGTTACA TTTGAGACAG TGAAGAAATT TTGTACAATC
    AGTGGAAAAG AAACTTTATC AGATCTGGTC ACAATGATAG CCAGCTGCAA GGAATTTCTA
    GATGTACAAT TAAGGATAAA TGAAAAGAAA ACACTGAATA CTTTGAACAA AGATCCAAAT
    CGGATAACTA TCAGATTTCC AATGGAAGGA AAAATTAAAA CAAGAGAAAT GAAAGTAAAT
    TGTCTTATTC AGGCTCAGCT AGGATGCATT CCCATACAAG ACTTTGCTTT GACACAAGAT
    ACCTCAAAGA TTTTCAGGAA TGGCTCACGA ATTACGAGAT GGTTATCAGA TTTTGTGGCT
    GTTCAAGAAA AGTTTGCTGT GCTTTTGAAC AGTTTGATTT TAGCTAAATG TTTTAGATGT
    AAACTTTGGG AAAACTCTCT GCATGTATCC AAACAACTGG AAAAAATCGG TATAACATTA
    TCAAATGCTA TGGTAAATGC AGGTTTGACT TCCTTTAAAA AAATAGAAGA AACAGATGCA
    AGGGAGCTTG AACTGATTTT AAATAGACAT CCCCCTTTTG GAACTCAGAT AAAAGAAACA
    GTGATGTGTC TGCCAAAATA CGAACTTGAA GTGGAACAGA TTGCAAGATA CAGTGATACC
    ACGGCAGAAG TTTTAGTGAC TGTTATATTA AGAAACTTCG AACCACTGCA AACTAAAAGA
    ACAGCACCAG ATTCTCACTA TGTTACCTTA ATCATAGGCG ATGCAGATAA TCAGCTGATT
    TTTAAGCATA AAATTATGGA TTCTGTTTTG CTGAAATCTG GAAATTGGGC TAAAAAGATC
    GATGTGAAGA GAGCTCTGAA ATCTGAAGAT CTTAGCATAA ATCTCATTAG TTCTGAGTAT
    GTTGGACTTG ATATTCAGCA GAAATTTACA GTCTTTTACT TAGGACCCAA GAGATTTGGA
    AATCAAATAA TTATGCAAAA AAAATCAGAA ACAGAGATCT TCCATTCTAA ACGTTCAGAT
    GGATCTCCTG TAGCAGGTCC CATTAAAGGA ATGACTGCCT GCAGAAAGCC TGGGCACCGA
    GAGTGTAACC ATCACTGTAA AAAGAAACAC ACGTGTGGAC ATGACTGCTG TAAAACTGGA
    GTTGCCCAGA AGCCAGAAAT GAAAGAGTCA ACAATTTCTT CTTATTTATC TGATTTAAGA
    AACAGGAATG CAGTTTCATC TCTTCCTCCA GTTAAACGTC TAAAGATGCA GATGAATAAA
    TCTCAAAGTG TGGACCTTAA AGAGTTTGGT TTTACTCCAC AACCTTCTCT TCCCAGTCTG
    TCAAGGTCAG AATGTTTAAA CATACCTAAA TTGCCTGTAA TGGAGCAGAG GGGTCAGCAC
    GAGCTCTATG CAGAAGGTCA ACAACAAGAA CCACCGGGAT ATCAGGACAA AGGTTTTTCC
    AGACAGACCT CTTCTACAAC CTCAAATGAG AAAACCCCTC AAACAACACT GTTCAACAGA
    AATATTAAGG CAACTGGAAA CCAGAATTTG ACAGCTGTGG TTAAAAGTAA TTCTTCCTAT
    TCAGAAGTTT TGAATGTGAA CTTTGAATTG GGAAATGAAG TTTGGGATGA TTTTGATGAT
    GAAAACTTAA TAGAAATTGC TAGCTTTTCA GCTGATACTG AAAAGACAAA AGCAGGATTT
    GGAAACACTT TGAGTTCAAG TGCCAGGGAA AATAAGCTAC CCTTCCAAGA ATCAAAAAGC
    AAATTCCAAA GAAAAATGTC AAACAGTTTT GTTTCATCAC ATGAGAAGCC AGATATTTAT
    TTACCAAATT CTGCTCTGTC CAAGATCAGT GCATCCTCCA TGACAAAATT ACCTCAACCA
    GCTGGAAATG TCCCTTTCCA AGAAATAAAA CAACAGAATC TGTCACCAGA GATTGAAAAG
    CTATGCTTTG CTCACTCTGA AAAAATATCA AGCTTTTCAA ATTCTGTTAC GCAAGTGGAT
    TGCTTTATTA GAAACAGTGA ATATAAAAAG CAACCTGACT TCAGTAGGTA TCATCTTGAT
    GATGAAGCTG ATGAAATGAA GTCTTTTCTG GGAATATTTG ATGGTATTTT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013852841.1
    CDS84..4397
    Translation

    Target ORF information:

    RefSeq Version XM_013997387.1
    Organism Sus scrofa(Pig)
    Definition Sus scrofa HFM1, ATP-dependent DNA helicase homolog (HFM1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_013997387.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGACTCCTT GTGTTCTTTG GATAATTTAT TTTTTGAAAA AACAGATGAA GCTGAAAAGT 
    CAGAAAAAGA GGTCAAAAAT GTTAACATCC AATTTAAAGA TAACTAATGA AGACACAAGT
    TATACTTCAC CAACACAAAA ATTCCAGTGT GCTTTCCCTT CTAATAAATA CGAAGAAGGC
    GATCTAAATT TAGTAGGGGC AGGTAGCAAT GACTTACATG TTGCTGGCAA GCTGACTTAT
    GGTTCTCCTC AGAAGTACAA AAGTCACATT GGCCCTGAGA TAGCACTTGA GAAAAATGTT
    CCAGATGATG CGAAAGTAGT TAATTTTGCA TACGATAAAG GAGAGAGCAC ATCGGCCTTC
    CGAAAAAGAT TATTTAAAAT ATCTGATAGT ATACATGGGA ATGCTTGTTC TAATGACATT
    GCAAACTTGG ACTCTGATAT TGGCTCAGCG AAGATTGCCC AAATGGAAAT GAGCGAAGGG
    AAATCGTGGA AATGGAACAA TAGTAATCAG AAACCTCAAT ATTCAGAAAT TAACATGTTT
    ACAGCCAGTG ATACTTTTTC TGCTTCTAAA ATCAGAGAAG GCATATTCAA AGCACCATCT
    TTTTCAGTTG CATCCCAGCC TCATGAAGTT CAAGGGATAA TAGAAAATGG TTTAGATTCC
    TTGAAGGCTG TCACAGAAAT CCCGGCAAAA TTTAGAAGTG TTTTCAAAGA ATTCCCATAT
    TTCAACTATA TCCAGTCTAA AGCCTTTGAT GATCTTCTTT ATACAGATAG GAATGTTGTG
    GTTTGTGCTC CAACTGGTTC TGGGAAAACT GTAATGTTTG AACTAGCTAT AACAAGATTG
    TTAATGGAAG TACCATTGCC ATGGTCAAAC ATTAAAATTG TTTACATGGC ACCAATAAAA
    GCCCTGTGCA GTCAGCGTTT TGATGACTGG AAAGAAAAAT TTGGACCAAT AGGATTGAAT
    TGTAAAGAAC TCACGGGAGA TACAGTCATG GATGACCTGT TTGAGATTCA GCATGCCCAT
    ATTATTATGA CAACTCCAGA AAAATGGGAT AGCATGACTA GAAAATGGAG AGACAACTCT
    TTGGTCCAAC TGGTTCGACT GTTTCTCATT GATGAGGTAC ATGTTGTAAA AGATGAAAAT
    CGTGGACCAA CTCTTGAAGT TGTTGTCAGC AGGATGAAAA CTGTGCAGTT TTTATCTCAT
    GCTGTGGAAA ATCCCAGAAA TATTATTCCA ATGAGATTTG TAGCTGTATC TGCAACAATT
    CCAAATGCTG AGGATATTGC AGAATGGCTT TCAGATGGTG AAAGATCCGC TATCTGTCTG
    AAAATGGATG AGAAACATAG ACCAGTGAAG CTTCGGAAAG TGGTCCTTGG TTTTCCCTGC
    AGTAATAACC AAACTGAATT TAAGTTTGAT TTAACCCTCA ACTACAAAAT TGCCAGTGTT
    GTACAAACGT ACTCCGATCA GAAACCCACA CTTGTGTTTT GTGCAACAAG GAAAGGTGTA
    CAACAGGCTG CTTCCATTCT TGTGAAAGAT GCCAAGTTTA TTATGACGGT GGAACAGAAA
    CAGAGGTTAC ATAAATGTGC ATATTCCATA AGAGATTCAA AATTGAGAGA TCTCTTAATA
    CACGGTGTTG CTTATCATCA TGCTGGCATG GAGCTGTCAG ATAGAAAAGT AGTCGAAGGA
    GCTTTTACTG TTGGAGATCT ACCAGTTCTT TTTACTACTA GTACCTTAGC TATGGGAGTA
    AATTTGCCTG CTCATCTAGT AGTTATCAAA TCTACAATGC ATTATGCTGG AGGGATGTTT
    GAAGAGTACA GTGAAACAGA TATTCTACAG ATGATTGGTA GAGCTGGTCG ACCTCAATTT
    GACACTACAG CAACTGCAGT TATTATGACT CGGTTAAGTA CAAAAGAGAA GTATATTCAG
    ATGCTGGCTT GTAGTGACAC TGTAGAAAGC AGTTTGCACA GACATCTTAT TGAGCATTTA
    AATGCAGAGA TAGTGTTGCA TACCATCACA GATGTCAACA TTGCTTTGGA ATGGATACGA
    TCAACCTTGC TGTATATCAG AGCCTTGAAG AATCCGTCTC ACTATGGTTT TGCATCTGGA
    TTGAACAAAG ATGGAATTGA ATCAAAATTA CAAGAATTGT GTTTGAAGAA TTTGAATGAT
    TTATCATCTC TGGACTTGAT AAAGATGGAT GAAGATATTA ATTTCAAGCC AACTGAGGCA
    GGAAGATTGA TGGCTTGGTA CTATGTTACA TTTGAGACAG TGAAGAAATT TTGTACAATC
    AGTGGAAAAG AAACTTTATC AGATCTGGTC ACAATGATAG CCAGCTGCAA GGAATTTCTA
    GATGTACAAT TAAGGATAAA TGAAAAGAAA ACACTGAATA CTTTGAACAA AGATCCAAAT
    CGGATAACTA TCAGATTTCC AATGGAAGGA AAAATTAAAA CAAGAGAAAT GAAAGTAAAT
    TGTCTTATTC AGGCTCAGCT AGGATGCATT CCCATACAAG ACTTTGCTTT GACACAAGAT
    ACCTCAAAGA TTTTCAGGAA TGGCTCACGA ATTACGAGAT GGTTATCAGA TTTTGTGGCT
    GTTCAAGAAA AGTTTGCTGT GCTTTTGAAC AGTTTGATTT TAGCTAAATG TTTTAGATGT
    AAACTTTGGG AAAACTCTCT GCATGTATCC AAACAACTGG AAAAAATCGG TATAACATTA
    TCAAATGCTA TGGTAAATGC AGGTTTGACT TCCTTTAAAA AAATAGAAGA AACAGATGCA
    AGGGAGCTTG AACTGATTTT AAATAGACAT CCCCCTTTTG GAACTCAGAT AAAAGAAACA
    GTGATGTGTC TGCCAAAATA CGAACTTGAA GTGGAACAGA TTGCAAGATA CAGTGATACC
    ACGGCAGAAG TTTTAGTGAC TGTTATATTA AGAAACTTCG AACCACTGCA AACTAAAAGA
    ACAGCACCAG ATTCTCACTA TGTTACCTTA ATCATAGGCG ATGCAGATAA TCAGCTGATT
    TTTAAGCATA AAATTATGGA TTCTGTTTTG CTGAAATCTG GAAATTGGGC TAAAAAGATC
    GATGTGAAGA GAGCTCTGAA ATCTGAAGAT CTTAGCATAA ATCTCATTAG TTCTGAGTAT
    GTTGGACTTG ATATTCAGCA GAAATTTACA GTCTTTTACT TAGGACCCAA GAGATTTGGA
    AATCAAATAA TTATGCAAAA AAAATCAGAA ACAGAGATCT TCCATTCTAA ACGTTCAGAT
    GGATCTCCTG TAGCAGGTCC CATTAAAGGA ATGACTGCCT GCAGAAAGCC TGGGCACCGA
    GAGTGTAACC ATCACTGTAA AAAGAAACAC ACGTGTGGAC ATGACTGCTG TAAAACTGGA
    GTTGCCCAGA AGCCAGAAAT GAAAGAGTCA ACAATTTCTT CTTATTTATC TGATTTAAGA
    AACAGGAATG CAGTTTCATC TCTTCCTCCA GTTAAACGTC TAAAGATGCA GATGAATAAA
    TCTCAAAGTG TGGACCTTAA AGAGTTTGGT TTTACTCCAC AACCTTCTCT TCCCAGTCTG
    TCAAGGTCAG AATGTTTAAA CATACCTAAA TTGCCTGTAA TGGAGCAGAG GGGTCAGCAC
    GAGCTCTATG CAGAAGGTCA ACAACAAGAA CCACCGGGAT ATCAGGACAA AGGTTTTTCC
    AGACAGACCT CTTCTACAAC CTCAAATGAG AAAACCCCTC AAACAACACT GTTCAACAGA
    AATATTAAGG CAACTGGAAA CCAGAATTTG ACAGCTGTGG TTAAAAGTAA TTCTTCCTAT
    TCAGAAGTTT TGAATGTGAA CTTTGAATTG GGAAATGAAG TTTGGGATGA TTTTGATGAT
    GAAAACTTAA TAGAAATTGC TAGCTTTTCA GCTGATACTG AAAAGACAAA AGCAGGATTT
    GGAAACACTT TGAGTTCAAG TGCCAGGGAA AATAAGCTAC CCTTCCAAGA ATCAAAAAGC
    AAATTCCAAA GAAAAATGTC AAACAGTTTT GTTTCATCAC ATGAGAAGCC AGATATTTAT
    TTACCAAATT CTGCTCTGTC CAAGATCAGT GCATCCTCCA TGACAAAATT ACCTCAACCA
    GCTGGAAATG TCCCTTTCCA AGAAATAAAA CAACAGAATC TGTCACCAGA GATTGAAAAG
    CTATGCTTTG CTCACTCTGA AAAAATATCA AGCTTTTCAA ATTCTGTTAC GCAAGTGGAT
    TGCTTTATTA GAAACAGTGA ATATAAAAAG CAACCTGACT TCAGTAGGTA TCATCTTGAT
    GATGAAGCTG ATGAAATGAA GTCTTTTCTG GGAATATTTG ATGGTATTTT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe35335
    Clone ID Related Accession (Same CDS sequence) XM_013997388.1
    Accession Version XM_013997388.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4098bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-10
    Organism Sus scrofa(Pig)
    Product probable ATP-dependent DNA helicase HFM1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003610184.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGCTGAGAT CACATGACTC CTTGTGTTCT TTGGATAATT TATTTTTTGA AAAAACAGAT 
    GAAGCTGAAA AATACCCAGA CAATGAAAAA TCATTAGATT GGTTTCTCCC ACCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAAGCA TTAGAGGAAG ACATGCAAAG TCATAAACTG
    TTAGGTCAGA AAAAGAGGTC AAAAATGTTA ACATCCAATT TAAAGATAAC TAATGAAGAC
    ACAAGTTATA CTTCACCAAC ACAAAAATTC CAGTGTGCTT TCCCTTCTAA TAAATACGAA
    GAAGGCGATC TAAATTTAGT AGGGGCAGGT AGCAATGACT TACATGTTGC TGGCAAGCTG
    ACTTATGGTT CTCCTCAGAA GTACAAAAGT CACATTGGCC CTGAGATAGC ACTTGAGAAA
    AATGTTCCAG ATGATGCGAA AGTAGTTAAT TTTGCATACG ATAAAGGAGA GAGCACATCG
    GCCTTCCGAA AAAGATTATT TAAAATATCT GATAGTATAC ATGGGAATGC TTGTTCTAAT
    GACATTGCAA ACTTGGACTC TGATATTGGC TCAGCGAAGA TTGCCCAAAT GGAAATGAGC
    GAAGGGAAAT CGTGGAAATG GAACAATAGT AATCAGAAAC CTCAATATTC AGAAATTAAC
    ATGTTTACAG CCAGTGATAC TTTTTCTGCT TCTAAAATCA GAGAAGGCAT ATTCAAAGCA
    CCATCTTTTT CAGTTGCATC CCAGCCTCAT GAAGTTCAAG GGATAATAGA AAATGGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATCCCG GCAAAATTTA GAAGTGTTTT CAAAGAATTC
    CCATATTTCA ACTATATCCA GTCTAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    GTTGTGGTTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAA TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGCAGTCA GCGTTTTGAT GACTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC GGGAGATACA GTCATGGATG ACCTGTTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCAACTGGT TCGACTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTT GTCAGCAGGA TGAAAACTGT GCAGTTTTTA
    TCTCATGCTG TGGAAAATCC CAGAAATATT ATTCCAATGA GATTTGTAGC TGTATCTGCA
    ACAATTCCAA ATGCTGAGGA TATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCCGCTATC
    TGTCTGAAAA TGGATGAGAA ACATAGACCA GTGAAGCTTC GGAAAGTGGT CCTTGGTTTT
    CCCTGCAGTA ATAACCAAAC TGAATTTAAG TTTGATTTAA CCCTCAACTA CAAAATTGCC
    AGTGTTGTAC AAACGTACTC CGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTACAAC AGGCTGCTTC CATTCTTGTG AAAGATGCCA AGTTTATTAT GACGGTGGAA
    CAGAAACAGA GGTTACATAA ATGTGCATAT TCCATAAGAG ATTCAAAATT GAGAGATCTC
    TTAATACACG GTGTTGCTTA TCATCATGCT GGCATGGAGC TGTCAGATAG AAAAGTAGTC
    GAAGGAGCTT TTACTGTTGG AGATCTACCA GTTCTTTTTA CTACTAGTAC CTTAGCTATG
    GGAGTAAATT TGCCTGCTCA TCTAGTAGTT ATCAAATCTA CAATGCATTA TGCTGGAGGG
    ATGTTTGAAG AGTACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCAAC TGCAGTTATT ATGACTCGGT TAAGTACAAA AGAGAAGTAT
    ATTCAGATGC TGGCTTGTAG TGACACTGTA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT GTTGCATACC ATCACAGATG TCAACATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTGTA TATCAGAGCC TTGAAGAATC CGTCTCACTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATTTG
    AATGATTTAT CATCTCTGGA CTTGATAAAG ATGGATGAAG ATATTAATTT CAAGCCAACT
    GAGGCAGGAA GATTGATGGC TTGGTACTAT GTTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATCAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG GATAAATGAA AAGAAAACAC TGAATACTTT GAACAAAGAT
    CCAAATCGGA TAACTATCAG ATTTCCAATG GAAGGAAAAA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCA TACAAGACTT TGCTTTGACA
    CAAGATACCT CAAAGATTTT CAGGAATGGC TCACGAATTA CGAGATGGTT ATCAGATTTT
    GTGGCTGTTC AAGAAAAGTT TGCTGTGCTT TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCTGCAT GTATCCAAAC AACTGGAAAA AATCGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AGCTTGAACT GATTTTAAAT AGACATCCCC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTGTCTGCC AAAATACGAA CTTGAAGTGG AACAGATTGC AAGATACAGT
    GATACCACGG CAGAAGTTTT AGTGACTGTT ATATTAAGAA ACTTCGAACC ACTGCAAACT
    AAAAGAACAG CACCAGATTC TCACTATGTT ACCTTAATCA TAGGCGATGC AGATAATCAG
    CTGATTTTTA AGCATAAAAT TATGGATTCT GTTTTGCTGA AATCTGGAAA TTGGGCTAAA
    AAGATCGATG TGAAGAGAGC TCTGAAATCT GAAGATCTTA GCATAAATCT CATTAGTTCT
    GAGTATGTTG GACTTGATAT TCAGCAGAAA TTTACAGTCT TTTACTTAGG ACCCAAGAGA
    TTTGGAAATC AAATAATTAT GCAAAAAAAA TCAGAAACAG AGATCTTCCA TTCTAAACGT
    TCAGATGGAT CTCCTGTAGC AGGTCCCATT AAAGGAATGA CTGCCTGCAG AAAGCCTGGG
    CACCGAGAGT GTAACCATCA CTGTAAAAAG AAACACACGT GTGGACATGA CTGCTGTAAA
    ACTGGAGTTG CCCAGAAGCC AGAAATGAAA GAGTCAACAA TTTCTTCTTA TTTATCTGAT
    TTAAGAAACA GGAATGCAGT TTCATCTCTT CCTCCAGTTA AACGTCTAAA GATGCAGATG
    AATAAATCTC AAAGTGTGGA CCTTAAAGAG TTTGGTTTTA CTCCACAACC TTCTCTTCCC
    AGTCTGTCAA GGTCAGAATG TTTAAACATA CCTAAATTGC CTGTAATGGA GCAGAGGGGT
    CAGCACGAGC TCTATGCAGA AGGTCAACAA CAAGAACCAC CGGGATATCA GGACAAAGGT
    TTTTCCAGAC AGACCTCTTC TACAACCTCA AATGAGAAAA CCCCTCAAAC AACACTGTTC
    AACAGAAATA TTAAGGCAAC TGGAAACCAG AATTTGACAG CTGTGGTTAA AAGTAATTCT
    TCCTATTCAG AAGTTTTGAA TGTGAACTTT GAATTGGGAA ATGAAGTTTG GGATGATTTT
    GATGATGAAA ACTTAATAGA AATTGCTAGC TTTTCAGCTG ATACTGAAAA GACAAAAGCA
    GTTTTGTTTC ATCACATGAG AAGCCAGATA TTTATTTACC AAATTCTGCT CTGTCCAAGA
    TCAGTGCATC CTCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013852842.1
    CDS91..4188
    Translation

    Target ORF information:

    RefSeq Version XM_013997388.1
    Organism Sus scrofa(Pig)
    Definition Sus scrofa HFM1, ATP-dependent DNA helicase homolog (HFM1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_013997388.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGCTGAGAT CACATGACTC CTTGTGTTCT TTGGATAATT TATTTTTTGA AAAAACAGAT 
    GAAGCTGAAA AATACCCAGA CAATGAAAAA TCATTAGATT GGTTTCTCCC ACCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAAGCA TTAGAGGAAG ACATGCAAAG TCATAAACTG
    TTAGGTCAGA AAAAGAGGTC AAAAATGTTA ACATCCAATT TAAAGATAAC TAATGAAGAC
    ACAAGTTATA CTTCACCAAC ACAAAAATTC CAGTGTGCTT TCCCTTCTAA TAAATACGAA
    GAAGGCGATC TAAATTTAGT AGGGGCAGGT AGCAATGACT TACATGTTGC TGGCAAGCTG
    ACTTATGGTT CTCCTCAGAA GTACAAAAGT CACATTGGCC CTGAGATAGC ACTTGAGAAA
    AATGTTCCAG ATGATGCGAA AGTAGTTAAT TTTGCATACG ATAAAGGAGA GAGCACATCG
    GCCTTCCGAA AAAGATTATT TAAAATATCT GATAGTATAC ATGGGAATGC TTGTTCTAAT
    GACATTGCAA ACTTGGACTC TGATATTGGC TCAGCGAAGA TTGCCCAAAT GGAAATGAGC
    GAAGGGAAAT CGTGGAAATG GAACAATAGT AATCAGAAAC CTCAATATTC AGAAATTAAC
    ATGTTTACAG CCAGTGATAC TTTTTCTGCT TCTAAAATCA GAGAAGGCAT ATTCAAAGCA
    CCATCTTTTT CAGTTGCATC CCAGCCTCAT GAAGTTCAAG GGATAATAGA AAATGGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATCCCG GCAAAATTTA GAAGTGTTTT CAAAGAATTC
    CCATATTTCA ACTATATCCA GTCTAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    GTTGTGGTTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAA TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGCAGTCA GCGTTTTGAT GACTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC GGGAGATACA GTCATGGATG ACCTGTTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCAACTGGT TCGACTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTT GTCAGCAGGA TGAAAACTGT GCAGTTTTTA
    TCTCATGCTG TGGAAAATCC CAGAAATATT ATTCCAATGA GATTTGTAGC TGTATCTGCA
    ACAATTCCAA ATGCTGAGGA TATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCCGCTATC
    TGTCTGAAAA TGGATGAGAA ACATAGACCA GTGAAGCTTC GGAAAGTGGT CCTTGGTTTT
    CCCTGCAGTA ATAACCAAAC TGAATTTAAG TTTGATTTAA CCCTCAACTA CAAAATTGCC
    AGTGTTGTAC AAACGTACTC CGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTACAAC AGGCTGCTTC CATTCTTGTG AAAGATGCCA AGTTTATTAT GACGGTGGAA
    CAGAAACAGA GGTTACATAA ATGTGCATAT TCCATAAGAG ATTCAAAATT GAGAGATCTC
    TTAATACACG GTGTTGCTTA TCATCATGCT GGCATGGAGC TGTCAGATAG AAAAGTAGTC
    GAAGGAGCTT TTACTGTTGG AGATCTACCA GTTCTTTTTA CTACTAGTAC CTTAGCTATG
    GGAGTAAATT TGCCTGCTCA TCTAGTAGTT ATCAAATCTA CAATGCATTA TGCTGGAGGG
    ATGTTTGAAG AGTACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCAAC TGCAGTTATT ATGACTCGGT TAAGTACAAA AGAGAAGTAT
    ATTCAGATGC TGGCTTGTAG TGACACTGTA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT GTTGCATACC ATCACAGATG TCAACATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTGTA TATCAGAGCC TTGAAGAATC CGTCTCACTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATTTG
    AATGATTTAT CATCTCTGGA CTTGATAAAG ATGGATGAAG ATATTAATTT CAAGCCAACT
    GAGGCAGGAA GATTGATGGC TTGGTACTAT GTTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATCAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG GATAAATGAA AAGAAAACAC TGAATACTTT GAACAAAGAT
    CCAAATCGGA TAACTATCAG ATTTCCAATG GAAGGAAAAA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCA TACAAGACTT TGCTTTGACA
    CAAGATACCT CAAAGATTTT CAGGAATGGC TCACGAATTA CGAGATGGTT ATCAGATTTT
    GTGGCTGTTC AAGAAAAGTT TGCTGTGCTT TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCTGCAT GTATCCAAAC AACTGGAAAA AATCGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AGCTTGAACT GATTTTAAAT AGACATCCCC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTGTCTGCC AAAATACGAA CTTGAAGTGG AACAGATTGC AAGATACAGT
    GATACCACGG CAGAAGTTTT AGTGACTGTT ATATTAAGAA ACTTCGAACC ACTGCAAACT
    AAAAGAACAG CACCAGATTC TCACTATGTT ACCTTAATCA TAGGCGATGC AGATAATCAG
    CTGATTTTTA AGCATAAAAT TATGGATTCT GTTTTGCTGA AATCTGGAAA TTGGGCTAAA
    AAGATCGATG TGAAGAGAGC TCTGAAATCT GAAGATCTTA GCATAAATCT CATTAGTTCT
    GAGTATGTTG GACTTGATAT TCAGCAGAAA TTTACAGTCT TTTACTTAGG ACCCAAGAGA
    TTTGGAAATC AAATAATTAT GCAAAAAAAA TCAGAAACAG AGATCTTCCA TTCTAAACGT
    TCAGATGGAT CTCCTGTAGC AGGTCCCATT AAAGGAATGA CTGCCTGCAG AAAGCCTGGG
    CACCGAGAGT GTAACCATCA CTGTAAAAAG AAACACACGT GTGGACATGA CTGCTGTAAA
    ACTGGAGTTG CCCAGAAGCC AGAAATGAAA GAGTCAACAA TTTCTTCTTA TTTATCTGAT
    TTAAGAAACA GGAATGCAGT TTCATCTCTT CCTCCAGTTA AACGTCTAAA GATGCAGATG
    AATAAATCTC AAAGTGTGGA CCTTAAAGAG TTTGGTTTTA CTCCACAACC TTCTCTTCCC
    AGTCTGTCAA GGTCAGAATG TTTAAACATA CCTAAATTGC CTGTAATGGA GCAGAGGGGT
    CAGCACGAGC TCTATGCAGA AGGTCAACAA CAAGAACCAC CGGGATATCA GGACAAAGGT
    TTTTCCAGAC AGACCTCTTC TACAACCTCA AATGAGAAAA CCCCTCAAAC AACACTGTTC
    AACAGAAATA TTAAGGCAAC TGGAAACCAG AATTTGACAG CTGTGGTTAA AAGTAATTCT
    TCCTATTCAG AAGTTTTGAA TGTGAACTTT GAATTGGGAA ATGAAGTTTG GGATGATTTT
    GATGATGAAA ACTTAATAGA AATTGCTAGC TTTTCAGCTG ATACTGAAAA GACAAAAGCA
    GTTTTGTTTC ATCACATGAG AAGCCAGATA TTTATTTACC AAATTCTGCT CTGTCCAAGA
    TCAGTGCATC CTCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe35336
    Clone ID Related Accession (Same CDS sequence) XM_013997389.1 , XM_013997390.1
    Accession Version XM_013997389.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4017bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-10
    Organism Sus scrofa(Pig)
    Product probable ATP-dependent DNA helicase HFM1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003610184.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCTGAGAT CACATGACTC CTTGTGTTCT TTGGATAATT TATTTTTTGA AAAAACAGAT 
    GAAGCTGAAA AATTATTTAA AATATCTGAT AGTATACATG GGAATGCTTG TTCTAATGAC
    ATTGCAAACT TGGACTCTGA TATTGGCTCA GCGAAGATTG CCCAAATGGA AATGAGCGAA
    GGGAAATCGT GGAAATGGAA CAATAGTAAT CAGAAACCTC AATATTCAGA AATTAACATG
    TTTACAGCCA GTGATACTTT TTCTGCTTCT AAAATCAGAG AAGGCATATT CAAAGCACCA
    TCTTTTTCAG TTGCATCCCA GCCTCATGAA GTTCAAGGGA TAATAGAAAA TGGTTTAGAT
    TCCTTGAAGG CTGTCACAGA AATCCCGGCA AAATTTAGAA GTGTTTTCAA AGAATTCCCA
    TATTTCAACT ATATCCAGTC TAAAGCCTTT GATGATCTTC TTTATACAGA TAGGAATGTT
    GTGGTTTGTG CTCCAACTGG TTCTGGGAAA ACTGTAATGT TTGAACTAGC TATAACAAGA
    TTGTTAATGG AAGTACCATT GCCATGGTCA AACATTAAAA TTGTTTACAT GGCACCAATA
    AAAGCCCTGT GCAGTCAGCG TTTTGATGAC TGGAAAGAAA AATTTGGACC AATAGGATTG
    AATTGTAAAG AACTCACGGG AGATACAGTC ATGGATGACC TGTTTGAGAT TCAGCATGCC
    CATATTATTA TGACAACTCC AGAAAAATGG GATAGCATGA CTAGAAAATG GAGAGACAAC
    TCTTTGGTCC AACTGGTTCG ACTGTTTCTC ATTGATGAGG TACATGTTGT AAAAGATGAA
    AATCGTGGAC CAACTCTTGA AGTTGTTGTC AGCAGGATGA AAACTGTGCA GTTTTTATCT
    CATGCTGTGG AAAATCCCAG AAATATTATT CCAATGAGAT TTGTAGCTGT ATCTGCAACA
    ATTCCAAATG CTGAGGATAT TGCAGAATGG CTTTCAGATG GTGAAAGATC CGCTATCTGT
    CTGAAAATGG ATGAGAAACA TAGACCAGTG AAGCTTCGGA AAGTGGTCCT TGGTTTTCCC
    TGCAGTAATA ACCAAACTGA ATTTAAGTTT GATTTAACCC TCAACTACAA AATTGCCAGT
    GTTGTACAAA CGTACTCCGA TCAGAAACCC ACACTTGTGT TTTGTGCAAC AAGGAAAGGT
    GTACAACAGG CTGCTTCCAT TCTTGTGAAA GATGCCAAGT TTATTATGAC GGTGGAACAG
    AAACAGAGGT TACATAAATG TGCATATTCC ATAAGAGATT CAAAATTGAG AGATCTCTTA
    ATACACGGTG TTGCTTATCA TCATGCTGGC ATGGAGCTGT CAGATAGAAA AGTAGTCGAA
    GGAGCTTTTA CTGTTGGAGA TCTACCAGTT CTTTTTACTA CTAGTACCTT AGCTATGGGA
    GTAAATTTGC CTGCTCATCT AGTAGTTATC AAATCTACAA TGCATTATGC TGGAGGGATG
    TTTGAAGAGT ACAGTGAAAC AGATATTCTA CAGATGATTG GTAGAGCTGG TCGACCTCAA
    TTTGACACTA CAGCAACTGC AGTTATTATG ACTCGGTTAA GTACAAAAGA GAAGTATATT
    CAGATGCTGG CTTGTAGTGA CACTGTAGAA AGCAGTTTGC ACAGACATCT TATTGAGCAT
    TTAAATGCAG AGATAGTGTT GCATACCATC ACAGATGTCA ACATTGCTTT GGAATGGATA
    CGATCAACCT TGCTGTATAT CAGAGCCTTG AAGAATCCGT CTCACTATGG TTTTGCATCT
    GGATTGAACA AAGATGGAAT TGAATCAAAA TTACAAGAAT TGTGTTTGAA GAATTTGAAT
    GATTTATCAT CTCTGGACTT GATAAAGATG GATGAAGATA TTAATTTCAA GCCAACTGAG
    GCAGGAAGAT TGATGGCTTG GTACTATGTT ACATTTGAGA CAGTGAAGAA ATTTTGTACA
    ATCAGTGGAA AAGAAACTTT ATCAGATCTG GTCACAATGA TAGCCAGCTG CAAGGAATTT
    CTAGATGTAC AATTAAGGAT AAATGAAAAG AAAACACTGA ATACTTTGAA CAAAGATCCA
    AATCGGATAA CTATCAGATT TCCAATGGAA GGAAAAATTA AAACAAGAGA AATGAAAGTA
    AATTGTCTTA TTCAGGCTCA GCTAGGATGC ATTCCCATAC AAGACTTTGC TTTGACACAA
    GATACCTCAA AGATTTTCAG GAATGGCTCA CGAATTACGA GATGGTTATC AGATTTTGTG
    GCTGTTCAAG AAAAGTTTGC TGTGCTTTTG AACAGTTTGA TTTTAGCTAA ATGTTTTAGA
    TGTAAACTTT GGGAAAACTC TCTGCATGTA TCCAAACAAC TGGAAAAAAT CGGTATAACA
    TTATCAAATG CTATGGTAAA TGCAGGTTTG ACTTCCTTTA AAAAAATAGA AGAAACAGAT
    GCAAGGGAGC TTGAACTGAT TTTAAATAGA CATCCCCCTT TTGGAACTCA GATAAAAGAA
    ACAGTGATGT GTCTGCCAAA ATACGAACTT GAAGTGGAAC AGATTGCAAG ATACAGTGAT
    ACCACGGCAG AAGTTTTAGT GACTGTTATA TTAAGAAACT TCGAACCACT GCAAACTAAA
    AGAACAGCAC CAGATTCTCA CTATGTTACC TTAATCATAG GCGATGCAGA TAATCAGCTG
    ATTTTTAAGC ATAAAATTAT GGATTCTGTT TTGCTGAAAT CTGGAAATTG GGCTAAAAAG
    ATCGATGTGA AGAGAGCTCT GAAATCTGAA GATCTTAGCA TAAATCTCAT TAGTTCTGAG
    TATGTTGGAC TTGATATTCA GCAGAAATTT ACAGTCTTTT ACTTAGGACC CAAGAGATTT
    GGAAATCAAA TAATTATGCA AAAAAAATCA GAAACAGAGA TCTTCCATTC TAAACGTTCA
    GATGGATCTC CTGTAGCAGG TCCCATTAAA GGAATGACTG CCTGCAGAAA GCCTGGGCAC
    CGAGAGTGTA ACCATCACTG TAAAAAGAAA CACACGTGTG GACATGACTG CTGTAAAACT
    GGAGTTGCCC AGAAGCCAGA AATGAAAGAG TCAACAATTT CTTCTTATTT ATCTGATTTA
    AGAAACAGGA ATGCAGTTTC ATCTCTTCCT CCAGTTAAAC GTCTAAAGAT GCAGATGAAT
    AAATCTCAAA GTGTGGACCT TAAAGAGTTT GGTTTTACTC CACAACCTTC TCTTCCCAGT
    CTGTCAAGGT CAGAATGTTT AAACATACCT AAATTGCCTG TAATGGAGCA GAGGGGTCAG
    CACGAGCTCT ATGCAGAAGG TCAACAACAA GAACCACCGG GATATCAGGA CAAAGGTTTT
    TCCAGACAGA CCTCTTCTAC AACCTCAAAT GAGAAAACCC CTCAAACAAC ACTGTTCAAC
    AGAAATATTA AGGCAACTGG AAACCAGAAT TTGACAGCTG TGGTTAAAAG TAATTCTTCC
    TATTCAGAAG TTTTGAATGT GAACTTTGAA TTGGGAAATG AAGTTTGGGA TGATTTTGAT
    GATGAAAACT TAATAGAAAT TGCTAGCTTT TCAGCTGATA CTGAAAAGAC AAAAGCAGGA
    TTTGGAAACA CTTTGAGTTC AAGTGCCAGG GAAAATAAGC TACCCTTCCA AGAATCAAAA
    AGCAAATTCC AAAGAAAAAT GTCAAACAGT TTTGTTTCAT CACATGAGAA GCCAGATATT
    TATTTACCAA ATTCTGCTCT GTCCAAGATC AGTGCATCCT CCATGACAAA ATTACCTCAA
    CCAGCTGGAA ATGTCCCTTT CCAAGAAATA AAACAACAGA ATCTGTCACC AGAGATTGAA
    AAGCTATGCT TTGCTCACTC TGAAAAAATA TCAAGCTTTT CAAATTCTGT TACGCAAGTG
    GATTGCTTTA TTAGAAACAG TGAATATAAA AAGCAACCTG ACTTCAGTAG GTATCATCTT
    GATGATGAAG CTGATGAAAT GAAGTCTTTT CTGGGAATAT TTGATGGTAT TTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013852843.1
    CDS91..4107
    Translation

    Target ORF information:

    RefSeq Version XM_013997389.1
    Organism Sus scrofa(Pig)
    Definition Sus scrofa HFM1, ATP-dependent DNA helicase homolog (HFM1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_013997389.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCTGAGAT CACATGACTC CTTGTGTTCT TTGGATAATT TATTTTTTGA AAAAACAGAT 
    GAAGCTGAAA AATTATTTAA AATATCTGAT AGTATACATG GGAATGCTTG TTCTAATGAC
    ATTGCAAACT TGGACTCTGA TATTGGCTCA GCGAAGATTG CCCAAATGGA AATGAGCGAA
    GGGAAATCGT GGAAATGGAA CAATAGTAAT CAGAAACCTC AATATTCAGA AATTAACATG
    TTTACAGCCA GTGATACTTT TTCTGCTTCT AAAATCAGAG AAGGCATATT CAAAGCACCA
    TCTTTTTCAG TTGCATCCCA GCCTCATGAA GTTCAAGGGA TAATAGAAAA TGGTTTAGAT
    TCCTTGAAGG CTGTCACAGA AATCCCGGCA AAATTTAGAA GTGTTTTCAA AGAATTCCCA
    TATTTCAACT ATATCCAGTC TAAAGCCTTT GATGATCTTC TTTATACAGA TAGGAATGTT
    GTGGTTTGTG CTCCAACTGG TTCTGGGAAA ACTGTAATGT TTGAACTAGC TATAACAAGA
    TTGTTAATGG AAGTACCATT GCCATGGTCA AACATTAAAA TTGTTTACAT GGCACCAATA
    AAAGCCCTGT GCAGTCAGCG TTTTGATGAC TGGAAAGAAA AATTTGGACC AATAGGATTG
    AATTGTAAAG AACTCACGGG AGATACAGTC ATGGATGACC TGTTTGAGAT TCAGCATGCC
    CATATTATTA TGACAACTCC AGAAAAATGG GATAGCATGA CTAGAAAATG GAGAGACAAC
    TCTTTGGTCC AACTGGTTCG ACTGTTTCTC ATTGATGAGG TACATGTTGT AAAAGATGAA
    AATCGTGGAC CAACTCTTGA AGTTGTTGTC AGCAGGATGA AAACTGTGCA GTTTTTATCT
    CATGCTGTGG AAAATCCCAG AAATATTATT CCAATGAGAT TTGTAGCTGT ATCTGCAACA
    ATTCCAAATG CTGAGGATAT TGCAGAATGG CTTTCAGATG GTGAAAGATC CGCTATCTGT
    CTGAAAATGG ATGAGAAACA TAGACCAGTG AAGCTTCGGA AAGTGGTCCT TGGTTTTCCC
    TGCAGTAATA ACCAAACTGA ATTTAAGTTT GATTTAACCC TCAACTACAA AATTGCCAGT
    GTTGTACAAA CGTACTCCGA TCAGAAACCC ACACTTGTGT TTTGTGCAAC AAGGAAAGGT
    GTACAACAGG CTGCTTCCAT TCTTGTGAAA GATGCCAAGT TTATTATGAC GGTGGAACAG
    AAACAGAGGT TACATAAATG TGCATATTCC ATAAGAGATT CAAAATTGAG AGATCTCTTA
    ATACACGGTG TTGCTTATCA TCATGCTGGC ATGGAGCTGT CAGATAGAAA AGTAGTCGAA
    GGAGCTTTTA CTGTTGGAGA TCTACCAGTT CTTTTTACTA CTAGTACCTT AGCTATGGGA
    GTAAATTTGC CTGCTCATCT AGTAGTTATC AAATCTACAA TGCATTATGC TGGAGGGATG
    TTTGAAGAGT ACAGTGAAAC AGATATTCTA CAGATGATTG GTAGAGCTGG TCGACCTCAA
    TTTGACACTA CAGCAACTGC AGTTATTATG ACTCGGTTAA GTACAAAAGA GAAGTATATT
    CAGATGCTGG CTTGTAGTGA CACTGTAGAA AGCAGTTTGC ACAGACATCT TATTGAGCAT
    TTAAATGCAG AGATAGTGTT GCATACCATC ACAGATGTCA ACATTGCTTT GGAATGGATA
    CGATCAACCT TGCTGTATAT CAGAGCCTTG AAGAATCCGT CTCACTATGG TTTTGCATCT
    GGATTGAACA AAGATGGAAT TGAATCAAAA TTACAAGAAT TGTGTTTGAA GAATTTGAAT
    GATTTATCAT CTCTGGACTT GATAAAGATG GATGAAGATA TTAATTTCAA GCCAACTGAG
    GCAGGAAGAT TGATGGCTTG GTACTATGTT ACATTTGAGA CAGTGAAGAA ATTTTGTACA
    ATCAGTGGAA AAGAAACTTT ATCAGATCTG GTCACAATGA TAGCCAGCTG CAAGGAATTT
    CTAGATGTAC AATTAAGGAT AAATGAAAAG AAAACACTGA ATACTTTGAA CAAAGATCCA
    AATCGGATAA CTATCAGATT TCCAATGGAA GGAAAAATTA AAACAAGAGA AATGAAAGTA
    AATTGTCTTA TTCAGGCTCA GCTAGGATGC ATTCCCATAC AAGACTTTGC TTTGACACAA
    GATACCTCAA AGATTTTCAG GAATGGCTCA CGAATTACGA GATGGTTATC AGATTTTGTG
    GCTGTTCAAG AAAAGTTTGC TGTGCTTTTG AACAGTTTGA TTTTAGCTAA ATGTTTTAGA
    TGTAAACTTT GGGAAAACTC TCTGCATGTA TCCAAACAAC TGGAAAAAAT CGGTATAACA
    TTATCAAATG CTATGGTAAA TGCAGGTTTG ACTTCCTTTA AAAAAATAGA AGAAACAGAT
    GCAAGGGAGC TTGAACTGAT TTTAAATAGA CATCCCCCTT TTGGAACTCA GATAAAAGAA
    ACAGTGATGT GTCTGCCAAA ATACGAACTT GAAGTGGAAC AGATTGCAAG ATACAGTGAT
    ACCACGGCAG AAGTTTTAGT GACTGTTATA TTAAGAAACT TCGAACCACT GCAAACTAAA
    AGAACAGCAC CAGATTCTCA CTATGTTACC TTAATCATAG GCGATGCAGA TAATCAGCTG
    ATTTTTAAGC ATAAAATTAT GGATTCTGTT TTGCTGAAAT CTGGAAATTG GGCTAAAAAG
    ATCGATGTGA AGAGAGCTCT GAAATCTGAA GATCTTAGCA TAAATCTCAT TAGTTCTGAG
    TATGTTGGAC TTGATATTCA GCAGAAATTT ACAGTCTTTT ACTTAGGACC CAAGAGATTT
    GGAAATCAAA TAATTATGCA AAAAAAATCA GAAACAGAGA TCTTCCATTC TAAACGTTCA
    GATGGATCTC CTGTAGCAGG TCCCATTAAA GGAATGACTG CCTGCAGAAA GCCTGGGCAC
    CGAGAGTGTA ACCATCACTG TAAAAAGAAA CACACGTGTG GACATGACTG CTGTAAAACT
    GGAGTTGCCC AGAAGCCAGA AATGAAAGAG TCAACAATTT CTTCTTATTT ATCTGATTTA
    AGAAACAGGA ATGCAGTTTC ATCTCTTCCT CCAGTTAAAC GTCTAAAGAT GCAGATGAAT
    AAATCTCAAA GTGTGGACCT TAAAGAGTTT GGTTTTACTC CACAACCTTC TCTTCCCAGT
    CTGTCAAGGT CAGAATGTTT AAACATACCT AAATTGCCTG TAATGGAGCA GAGGGGTCAG
    CACGAGCTCT ATGCAGAAGG TCAACAACAA GAACCACCGG GATATCAGGA CAAAGGTTTT
    TCCAGACAGA CCTCTTCTAC AACCTCAAAT GAGAAAACCC CTCAAACAAC ACTGTTCAAC
    AGAAATATTA AGGCAACTGG AAACCAGAAT TTGACAGCTG TGGTTAAAAG TAATTCTTCC
    TATTCAGAAG TTTTGAATGT GAACTTTGAA TTGGGAAATG AAGTTTGGGA TGATTTTGAT
    GATGAAAACT TAATAGAAAT TGCTAGCTTT TCAGCTGATA CTGAAAAGAC AAAAGCAGGA
    TTTGGAAACA CTTTGAGTTC AAGTGCCAGG GAAAATAAGC TACCCTTCCA AGAATCAAAA
    AGCAAATTCC AAAGAAAAAT GTCAAACAGT TTTGTTTCAT CACATGAGAA GCCAGATATT
    TATTTACCAA ATTCTGCTCT GTCCAAGATC AGTGCATCCT CCATGACAAA ATTACCTCAA
    CCAGCTGGAA ATGTCCCTTT CCAAGAAATA AAACAACAGA ATCTGTCACC AGAGATTGAA
    AAGCTATGCT TTGCTCACTC TGAAAAAATA TCAAGCTTTT CAAATTCTGT TACGCAAGTG
    GATTGCTTTA TTAGAAACAG TGAATATAAA AAGCAACCTG ACTTCAGTAG GTATCATCTT
    GATGATGAAG CTGATGAAAT GAAGTCTTTT CTGGGAATAT TTGATGGTAT TTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe35336
    Clone ID Related Accession (Same CDS sequence) XM_013997389.1 , XM_013997390.1
    Accession Version XM_013997390.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4017bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-10
    Organism Sus scrofa(Pig)
    Product probable ATP-dependent DNA helicase HFM1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003610184.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCTGAGAT CACATGACTC CTTGTGTTCT TTGGATAATT TATTTTTTGA AAAAACAGAT 
    GAAGCTGAAA AATTATTTAA AATATCTGAT AGTATACATG GGAATGCTTG TTCTAATGAC
    ATTGCAAACT TGGACTCTGA TATTGGCTCA GCGAAGATTG CCCAAATGGA AATGAGCGAA
    GGGAAATCGT GGAAATGGAA CAATAGTAAT CAGAAACCTC AATATTCAGA AATTAACATG
    TTTACAGCCA GTGATACTTT TTCTGCTTCT AAAATCAGAG AAGGCATATT CAAAGCACCA
    TCTTTTTCAG TTGCATCCCA GCCTCATGAA GTTCAAGGGA TAATAGAAAA TGGTTTAGAT
    TCCTTGAAGG CTGTCACAGA AATCCCGGCA AAATTTAGAA GTGTTTTCAA AGAATTCCCA
    TATTTCAACT ATATCCAGTC TAAAGCCTTT GATGATCTTC TTTATACAGA TAGGAATGTT
    GTGGTTTGTG CTCCAACTGG TTCTGGGAAA ACTGTAATGT TTGAACTAGC TATAACAAGA
    TTGTTAATGG AAGTACCATT GCCATGGTCA AACATTAAAA TTGTTTACAT GGCACCAATA
    AAAGCCCTGT GCAGTCAGCG TTTTGATGAC TGGAAAGAAA AATTTGGACC AATAGGATTG
    AATTGTAAAG AACTCACGGG AGATACAGTC ATGGATGACC TGTTTGAGAT TCAGCATGCC
    CATATTATTA TGACAACTCC AGAAAAATGG GATAGCATGA CTAGAAAATG GAGAGACAAC
    TCTTTGGTCC AACTGGTTCG ACTGTTTCTC ATTGATGAGG TACATGTTGT AAAAGATGAA
    AATCGTGGAC CAACTCTTGA AGTTGTTGTC AGCAGGATGA AAACTGTGCA GTTTTTATCT
    CATGCTGTGG AAAATCCCAG AAATATTATT CCAATGAGAT TTGTAGCTGT ATCTGCAACA
    ATTCCAAATG CTGAGGATAT TGCAGAATGG CTTTCAGATG GTGAAAGATC CGCTATCTGT
    CTGAAAATGG ATGAGAAACA TAGACCAGTG AAGCTTCGGA AAGTGGTCCT TGGTTTTCCC
    TGCAGTAATA ACCAAACTGA ATTTAAGTTT GATTTAACCC TCAACTACAA AATTGCCAGT
    GTTGTACAAA CGTACTCCGA TCAGAAACCC ACACTTGTGT TTTGTGCAAC AAGGAAAGGT
    GTACAACAGG CTGCTTCCAT TCTTGTGAAA GATGCCAAGT TTATTATGAC GGTGGAACAG
    AAACAGAGGT TACATAAATG TGCATATTCC ATAAGAGATT CAAAATTGAG AGATCTCTTA
    ATACACGGTG TTGCTTATCA TCATGCTGGC ATGGAGCTGT CAGATAGAAA AGTAGTCGAA
    GGAGCTTTTA CTGTTGGAGA TCTACCAGTT CTTTTTACTA CTAGTACCTT AGCTATGGGA
    GTAAATTTGC CTGCTCATCT AGTAGTTATC AAATCTACAA TGCATTATGC TGGAGGGATG
    TTTGAAGAGT ACAGTGAAAC AGATATTCTA CAGATGATTG GTAGAGCTGG TCGACCTCAA
    TTTGACACTA CAGCAACTGC AGTTATTATG ACTCGGTTAA GTACAAAAGA GAAGTATATT
    CAGATGCTGG CTTGTAGTGA CACTGTAGAA AGCAGTTTGC ACAGACATCT TATTGAGCAT
    TTAAATGCAG AGATAGTGTT GCATACCATC ACAGATGTCA ACATTGCTTT GGAATGGATA
    CGATCAACCT TGCTGTATAT CAGAGCCTTG AAGAATCCGT CTCACTATGG TTTTGCATCT
    GGATTGAACA AAGATGGAAT TGAATCAAAA TTACAAGAAT TGTGTTTGAA GAATTTGAAT
    GATTTATCAT CTCTGGACTT GATAAAGATG GATGAAGATA TTAATTTCAA GCCAACTGAG
    GCAGGAAGAT TGATGGCTTG GTACTATGTT ACATTTGAGA CAGTGAAGAA ATTTTGTACA
    ATCAGTGGAA AAGAAACTTT ATCAGATCTG GTCACAATGA TAGCCAGCTG CAAGGAATTT
    CTAGATGTAC AATTAAGGAT AAATGAAAAG AAAACACTGA ATACTTTGAA CAAAGATCCA
    AATCGGATAA CTATCAGATT TCCAATGGAA GGAAAAATTA AAACAAGAGA AATGAAAGTA
    AATTGTCTTA TTCAGGCTCA GCTAGGATGC ATTCCCATAC AAGACTTTGC TTTGACACAA
    GATACCTCAA AGATTTTCAG GAATGGCTCA CGAATTACGA GATGGTTATC AGATTTTGTG
    GCTGTTCAAG AAAAGTTTGC TGTGCTTTTG AACAGTTTGA TTTTAGCTAA ATGTTTTAGA
    TGTAAACTTT GGGAAAACTC TCTGCATGTA TCCAAACAAC TGGAAAAAAT CGGTATAACA
    TTATCAAATG CTATGGTAAA TGCAGGTTTG ACTTCCTTTA AAAAAATAGA AGAAACAGAT
    GCAAGGGAGC TTGAACTGAT TTTAAATAGA CATCCCCCTT TTGGAACTCA GATAAAAGAA
    ACAGTGATGT GTCTGCCAAA ATACGAACTT GAAGTGGAAC AGATTGCAAG ATACAGTGAT
    ACCACGGCAG AAGTTTTAGT GACTGTTATA TTAAGAAACT TCGAACCACT GCAAACTAAA
    AGAACAGCAC CAGATTCTCA CTATGTTACC TTAATCATAG GCGATGCAGA TAATCAGCTG
    ATTTTTAAGC ATAAAATTAT GGATTCTGTT TTGCTGAAAT CTGGAAATTG GGCTAAAAAG
    ATCGATGTGA AGAGAGCTCT GAAATCTGAA GATCTTAGCA TAAATCTCAT TAGTTCTGAG
    TATGTTGGAC TTGATATTCA GCAGAAATTT ACAGTCTTTT ACTTAGGACC CAAGAGATTT
    GGAAATCAAA TAATTATGCA AAAAAAATCA GAAACAGAGA TCTTCCATTC TAAACGTTCA
    GATGGATCTC CTGTAGCAGG TCCCATTAAA GGAATGACTG CCTGCAGAAA GCCTGGGCAC
    CGAGAGTGTA ACCATCACTG TAAAAAGAAA CACACGTGTG GACATGACTG CTGTAAAACT
    GGAGTTGCCC AGAAGCCAGA AATGAAAGAG TCAACAATTT CTTCTTATTT ATCTGATTTA
    AGAAACAGGA ATGCAGTTTC ATCTCTTCCT CCAGTTAAAC GTCTAAAGAT GCAGATGAAT
    AAATCTCAAA GTGTGGACCT TAAAGAGTTT GGTTTTACTC CACAACCTTC TCTTCCCAGT
    CTGTCAAGGT CAGAATGTTT AAACATACCT AAATTGCCTG TAATGGAGCA GAGGGGTCAG
    CACGAGCTCT ATGCAGAAGG TCAACAACAA GAACCACCGG GATATCAGGA CAAAGGTTTT
    TCCAGACAGA CCTCTTCTAC AACCTCAAAT GAGAAAACCC CTCAAACAAC ACTGTTCAAC
    AGAAATATTA AGGCAACTGG AAACCAGAAT TTGACAGCTG TGGTTAAAAG TAATTCTTCC
    TATTCAGAAG TTTTGAATGT GAACTTTGAA TTGGGAAATG AAGTTTGGGA TGATTTTGAT
    GATGAAAACT TAATAGAAAT TGCTAGCTTT TCAGCTGATA CTGAAAAGAC AAAAGCAGGA
    TTTGGAAACA CTTTGAGTTC AAGTGCCAGG GAAAATAAGC TACCCTTCCA AGAATCAAAA
    AGCAAATTCC AAAGAAAAAT GTCAAACAGT TTTGTTTCAT CACATGAGAA GCCAGATATT
    TATTTACCAA ATTCTGCTCT GTCCAAGATC AGTGCATCCT CCATGACAAA ATTACCTCAA
    CCAGCTGGAA ATGTCCCTTT CCAAGAAATA AAACAACAGA ATCTGTCACC AGAGATTGAA
    AAGCTATGCT TTGCTCACTC TGAAAAAATA TCAAGCTTTT CAAATTCTGT TACGCAAGTG
    GATTGCTTTA TTAGAAACAG TGAATATAAA AAGCAACCTG ACTTCAGTAG GTATCATCTT
    GATGATGAAG CTGATGAAAT GAAGTCTTTT CTGGGAATAT TTGATGGTAT TTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013852844.1
    CDS404..4420
    Translation

    Target ORF information:

    RefSeq Version XM_013997390.1
    Organism Sus scrofa(Pig)
    Definition Sus scrofa HFM1, ATP-dependent DNA helicase homolog (HFM1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_013997390.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCTGAGAT CACATGACTC CTTGTGTTCT TTGGATAATT TATTTTTTGA AAAAACAGAT 
    GAAGCTGAAA AATTATTTAA AATATCTGAT AGTATACATG GGAATGCTTG TTCTAATGAC
    ATTGCAAACT TGGACTCTGA TATTGGCTCA GCGAAGATTG CCCAAATGGA AATGAGCGAA
    GGGAAATCGT GGAAATGGAA CAATAGTAAT CAGAAACCTC AATATTCAGA AATTAACATG
    TTTACAGCCA GTGATACTTT TTCTGCTTCT AAAATCAGAG AAGGCATATT CAAAGCACCA
    TCTTTTTCAG TTGCATCCCA GCCTCATGAA GTTCAAGGGA TAATAGAAAA TGGTTTAGAT
    TCCTTGAAGG CTGTCACAGA AATCCCGGCA AAATTTAGAA GTGTTTTCAA AGAATTCCCA
    TATTTCAACT ATATCCAGTC TAAAGCCTTT GATGATCTTC TTTATACAGA TAGGAATGTT
    GTGGTTTGTG CTCCAACTGG TTCTGGGAAA ACTGTAATGT TTGAACTAGC TATAACAAGA
    TTGTTAATGG AAGTACCATT GCCATGGTCA AACATTAAAA TTGTTTACAT GGCACCAATA
    AAAGCCCTGT GCAGTCAGCG TTTTGATGAC TGGAAAGAAA AATTTGGACC AATAGGATTG
    AATTGTAAAG AACTCACGGG AGATACAGTC ATGGATGACC TGTTTGAGAT TCAGCATGCC
    CATATTATTA TGACAACTCC AGAAAAATGG GATAGCATGA CTAGAAAATG GAGAGACAAC
    TCTTTGGTCC AACTGGTTCG ACTGTTTCTC ATTGATGAGG TACATGTTGT AAAAGATGAA
    AATCGTGGAC CAACTCTTGA AGTTGTTGTC AGCAGGATGA AAACTGTGCA GTTTTTATCT
    CATGCTGTGG AAAATCCCAG AAATATTATT CCAATGAGAT TTGTAGCTGT ATCTGCAACA
    ATTCCAAATG CTGAGGATAT TGCAGAATGG CTTTCAGATG GTGAAAGATC CGCTATCTGT
    CTGAAAATGG ATGAGAAACA TAGACCAGTG AAGCTTCGGA AAGTGGTCCT TGGTTTTCCC
    TGCAGTAATA ACCAAACTGA ATTTAAGTTT GATTTAACCC TCAACTACAA AATTGCCAGT
    GTTGTACAAA CGTACTCCGA TCAGAAACCC ACACTTGTGT TTTGTGCAAC AAGGAAAGGT
    GTACAACAGG CTGCTTCCAT TCTTGTGAAA GATGCCAAGT TTATTATGAC GGTGGAACAG
    AAACAGAGGT TACATAAATG TGCATATTCC ATAAGAGATT CAAAATTGAG AGATCTCTTA
    ATACACGGTG TTGCTTATCA TCATGCTGGC ATGGAGCTGT CAGATAGAAA AGTAGTCGAA
    GGAGCTTTTA CTGTTGGAGA TCTACCAGTT CTTTTTACTA CTAGTACCTT AGCTATGGGA
    GTAAATTTGC CTGCTCATCT AGTAGTTATC AAATCTACAA TGCATTATGC TGGAGGGATG
    TTTGAAGAGT ACAGTGAAAC AGATATTCTA CAGATGATTG GTAGAGCTGG TCGACCTCAA
    TTTGACACTA CAGCAACTGC AGTTATTATG ACTCGGTTAA GTACAAAAGA GAAGTATATT
    CAGATGCTGG CTTGTAGTGA CACTGTAGAA AGCAGTTTGC ACAGACATCT TATTGAGCAT
    TTAAATGCAG AGATAGTGTT GCATACCATC ACAGATGTCA ACATTGCTTT GGAATGGATA
    CGATCAACCT TGCTGTATAT CAGAGCCTTG AAGAATCCGT CTCACTATGG TTTTGCATCT
    GGATTGAACA AAGATGGAAT TGAATCAAAA TTACAAGAAT TGTGTTTGAA GAATTTGAAT
    GATTTATCAT CTCTGGACTT GATAAAGATG GATGAAGATA TTAATTTCAA GCCAACTGAG
    GCAGGAAGAT TGATGGCTTG GTACTATGTT ACATTTGAGA CAGTGAAGAA ATTTTGTACA
    ATCAGTGGAA AAGAAACTTT ATCAGATCTG GTCACAATGA TAGCCAGCTG CAAGGAATTT
    CTAGATGTAC AATTAAGGAT AAATGAAAAG AAAACACTGA ATACTTTGAA CAAAGATCCA
    AATCGGATAA CTATCAGATT TCCAATGGAA GGAAAAATTA AAACAAGAGA AATGAAAGTA
    AATTGTCTTA TTCAGGCTCA GCTAGGATGC ATTCCCATAC AAGACTTTGC TTTGACACAA
    GATACCTCAA AGATTTTCAG GAATGGCTCA CGAATTACGA GATGGTTATC AGATTTTGTG
    GCTGTTCAAG AAAAGTTTGC TGTGCTTTTG AACAGTTTGA TTTTAGCTAA ATGTTTTAGA
    TGTAAACTTT GGGAAAACTC TCTGCATGTA TCCAAACAAC TGGAAAAAAT CGGTATAACA
    TTATCAAATG CTATGGTAAA TGCAGGTTTG ACTTCCTTTA AAAAAATAGA AGAAACAGAT
    GCAAGGGAGC TTGAACTGAT TTTAAATAGA CATCCCCCTT TTGGAACTCA GATAAAAGAA
    ACAGTGATGT GTCTGCCAAA ATACGAACTT GAAGTGGAAC AGATTGCAAG ATACAGTGAT
    ACCACGGCAG AAGTTTTAGT GACTGTTATA TTAAGAAACT TCGAACCACT GCAAACTAAA
    AGAACAGCAC CAGATTCTCA CTATGTTACC TTAATCATAG GCGATGCAGA TAATCAGCTG
    ATTTTTAAGC ATAAAATTAT GGATTCTGTT TTGCTGAAAT CTGGAAATTG GGCTAAAAAG
    ATCGATGTGA AGAGAGCTCT GAAATCTGAA GATCTTAGCA TAAATCTCAT TAGTTCTGAG
    TATGTTGGAC TTGATATTCA GCAGAAATTT ACAGTCTTTT ACTTAGGACC CAAGAGATTT
    GGAAATCAAA TAATTATGCA AAAAAAATCA GAAACAGAGA TCTTCCATTC TAAACGTTCA
    GATGGATCTC CTGTAGCAGG TCCCATTAAA GGAATGACTG CCTGCAGAAA GCCTGGGCAC
    CGAGAGTGTA ACCATCACTG TAAAAAGAAA CACACGTGTG GACATGACTG CTGTAAAACT
    GGAGTTGCCC AGAAGCCAGA AATGAAAGAG TCAACAATTT CTTCTTATTT ATCTGATTTA
    AGAAACAGGA ATGCAGTTTC ATCTCTTCCT CCAGTTAAAC GTCTAAAGAT GCAGATGAAT
    AAATCTCAAA GTGTGGACCT TAAAGAGTTT GGTTTTACTC CACAACCTTC TCTTCCCAGT
    CTGTCAAGGT CAGAATGTTT AAACATACCT AAATTGCCTG TAATGGAGCA GAGGGGTCAG
    CACGAGCTCT ATGCAGAAGG TCAACAACAA GAACCACCGG GATATCAGGA CAAAGGTTTT
    TCCAGACAGA CCTCTTCTAC AACCTCAAAT GAGAAAACCC CTCAAACAAC ACTGTTCAAC
    AGAAATATTA AGGCAACTGG AAACCAGAAT TTGACAGCTG TGGTTAAAAG TAATTCTTCC
    TATTCAGAAG TTTTGAATGT GAACTTTGAA TTGGGAAATG AAGTTTGGGA TGATTTTGAT
    GATGAAAACT TAATAGAAAT TGCTAGCTTT TCAGCTGATA CTGAAAAGAC AAAAGCAGGA
    TTTGGAAACA CTTTGAGTTC AAGTGCCAGG GAAAATAAGC TACCCTTCCA AGAATCAAAA
    AGCAAATTCC AAAGAAAAAT GTCAAACAGT TTTGTTTCAT CACATGAGAA GCCAGATATT
    TATTTACCAA ATTCTGCTCT GTCCAAGATC AGTGCATCCT CCATGACAAA ATTACCTCAA
    CCAGCTGGAA ATGTCCCTTT CCAAGAAATA AAACAACAGA ATCTGTCACC AGAGATTGAA
    AAGCTATGCT TTGCTCACTC TGAAAAAATA TCAAGCTTTT CAAATTCTGT TACGCAAGTG
    GATTGCTTTA TTAGAAACAG TGAATATAAA AAGCAACCTG ACTTCAGTAG GTATCATCTT
    GATGATGAAG CTGATGAAAT GAAGTCTTTT CTGGGAATAT TTGATGGTAT TTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe09310
    Clone ID Related Accession (Same CDS sequence) XM_013997386.1
    Accession Version XM_013997386.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4437bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-10
    Organism Sus scrofa(Pig)
    Product probable ATP-dependent DNA helicase HFM1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003610184.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCTGAGAT CACATGACTC CTTGTGTTCT TTGGATAATT TATTTTTTGA AAAAACAGAT 
    GAAGCTGAAA AATACCCAGA CAATGAAAAA TCATTAGATT GGTTTCTCCC ACCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAAGCA TTAGAGGAAG ACATGCAAAG TCATAAACTG
    TTAGGTCAGA AAAAGAGGTC AAAAATGTTA ACATCCAATT TAAAGATAAC TAATGAAGAC
    ACAAGTTATA CTTCACCAAC ACAAAAATTC CAGTGTGCTT TCCCTTCTAA TAAATACGAA
    GAAGGCGATC TAAATTTAGT AGGGGCAGGT AGCAATGACT TACATGTTGC TGGCAAGCTG
    ACTTATGGTT CTCCTCAGAA GTACAAAAGT CACATTGGCC CTGAGATAGC ACTTGAGAAA
    AATGTTCCAG ATGATGCGAA AGTAGTTAAT TTTGCATACG ATAAAGGAGA GAGCACATCG
    GCCTTCCGAA AAAGATTATT TAAAATATCT GATAGTATAC ATGGGAATGC TTGTTCTAAT
    GACATTGCAA ACTTGGACTC TGATATTGGC TCAGCGAAGA TTGCCCAAAT GGAAATGAGC
    GAAGGGAAAT CGTGGAAATG GAACAATAGT AATCAGAAAC CTCAATATTC AGAAATTAAC
    ATGTTTACAG CCAGTGATAC TTTTTCTGCT TCTAAAATCA GAGAAGGCAT ATTCAAAGCA
    CCATCTTTTT CAGTTGCATC CCAGCCTCAT GAAGTTCAAG GGATAATAGA AAATGGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATCCCG GCAAAATTTA GAAGTGTTTT CAAAGAATTC
    CCATATTTCA ACTATATCCA GTCTAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    GTTGTGGTTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAA TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGCAGTCA GCGTTTTGAT GACTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC GGGAGATACA GTCATGGATG ACCTGTTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCAACTGGT TCGACTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTT GTCAGCAGGA TGAAAACTGT GCAGTTTTTA
    TCTCATGCTG TGGAAAATCC CAGAAATATT ATTCCAATGA GATTTGTAGC TGTATCTGCA
    ACAATTCCAA ATGCTGAGGA TATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCCGCTATC
    TGTCTGAAAA TGGATGAGAA ACATAGACCA GTGAAGCTTC GGAAAGTGGT CCTTGGTTTT
    CCCTGCAGTA ATAACCAAAC TGAATTTAAG TTTGATTTAA CCCTCAACTA CAAAATTGCC
    AGTGTTGTAC AAACGTACTC CGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTACAAC AGGCTGCTTC CATTCTTGTG AAAGATGCCA AGTTTATTAT GACGGTGGAA
    CAGAAACAGA GGTTACATAA ATGTGCATAT TCCATAAGAG ATTCAAAATT GAGAGATCTC
    TTAATACACG GTGTTGCTTA TCATCATGCT GGCATGGAGC TGTCAGATAG AAAAGTAGTC
    GAAGGAGCTT TTACTGTTGG AGATCTACCA GTTCTTTTTA CTACTAGTAC CTTAGCTATG
    GGAGTAAATT TGCCTGCTCA TCTAGTAGTT ATCAAATCTA CAATGCATTA TGCTGGAGGG
    ATGTTTGAAG AGTACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCAAC TGCAGTTATT ATGACTCGGT TAAGTACAAA AGAGAAGTAT
    ATTCAGATGC TGGCTTGTAG TGACACTGTA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT GTTGCATACC ATCACAGATG TCAACATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTGTA TATCAGAGCC TTGAAGAATC CGTCTCACTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATTTG
    AATGATTTAT CATCTCTGGA CTTGATAAAG ATGGATGAAG ATATTAATTT CAAGCCAACT
    GAGGCAGGAA GATTGATGGC TTGGTACTAT GTTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATCAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG GATAAATGAA AAGAAAACAC TGAATACTTT GAACAAAGAT
    CCAAATCGGA TAACTATCAG ATTTCCAATG GAAGGAAAAA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCA TACAAGACTT TGCTTTGACA
    CAAGATACCT CAAAGATTTT CAGGAATGGC TCACGAATTA CGAGATGGTT ATCAGATTTT
    GTGGCTGTTC AAGAAAAGTT TGCTGTGCTT TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCTGCAT GTATCCAAAC AACTGGAAAA AATCGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AGCTTGAACT GATTTTAAAT AGACATCCCC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTGTCTGCC AAAATACGAA CTTGAAGTGG AACAGATTGC AAGATACAGT
    GATACCACGG CAGAAGTTTT AGTGACTGTT ATATTAAGAA ACTTCGAACC ACTGCAAACT
    AAAAGAACAG CACCAGATTC TCACTATGTT ACCTTAATCA TAGGCGATGC AGATAATCAG
    CTGATTTTTA AGCATAAAAT TATGGATTCT GTTTTGCTGA AATCTGGAAA TTGGGCTAAA
    AAGATCGATG TGAAGAGAGC TCTGAAATCT GAAGATCTTA GCATAAATCT CATTAGTTCT
    GAGTATGTTG GACTTGATAT TCAGCAGAAA TTTACAGTCT TTTACTTAGG ACCCAAGAGA
    TTTGGAAATC AAATAATTAT GCAAAAAAAA TCAGAAACAG AGATCTTCCA TTCTAAACGT
    TCAGATGGAT CTCCTGTAGC AGGTCCCATT AAAGGAATGA CTGCCTGCAG AAAGCCTGGG
    CACCGAGAGT GTAACCATCA CTGTAAAAAG AAACACACGT GTGGACATGA CTGCTGTAAA
    ACTGGAGTTG CCCAGAAGCC AGAAATGAAA GAGTCAACAA TTTCTTCTTA TTTATCTGAT
    TTAAGAAACA GGAATGCAGT TTCATCTCTT CCTCCAGTTA AACGTCTAAA GATGCAGATG
    AATAAATCTC AAAGTGTGGA CCTTAAAGAG TTTGGTTTTA CTCCACAACC TTCTCTTCCC
    AGTCTGTCAA GGTCAGAATG TTTAAACATA CCTAAATTGC CTGTAATGGA GCAGAGGGGT
    CAGCACGAGC TCTATGCAGA AGGTCAACAA CAAGAACCAC CGGGATATCA GGACAAAGGT
    TTTTCCAGAC AGACCTCTTC TACAACCTCA AATGAGAAAA CCCCTCAAAC AACACTGTTC
    AACAGAAATA TTAAGGCAAC TGGAAACCAG AATTTGACAG CTGTGGTTAA AAGTAATTCT
    TCCTATTCAG AAGTTTTGAA TGTGAACTTT GAATTGGGAA ATGAAGTTTG GGATGATTTT
    GATGATGAAA ACTTAATAGA AATTGCTAGC TTTTCAGCTG ATACTGAAAA GACAAAAGCA
    GGATTTGGAA ACACTTTGAG TTCAAGTGCC AGGGAAAATA AGCTACCCTT CCAAGAATCA
    AAAAGCAAAT TCCAAAGAAA AATGTCAAAC AGTTTTGTTT CATCACATGA GAAGCCAGAT
    ATTTATTTAC CAAATTCTGC TCTGTCCAAG ATCAGTGCAT CCTCCATGAC AAAATTACCT
    CAACCAGCTG GAAATGTCCC TTTCCAAGAA ATAAAACAAC AGAATCTGTC ACCAGAGATT
    GAAAAGCTAT GCTTTGCTCA CTCTGAAAAA ATATCAAGCT TTTCAAATTC TGTTACGCAA
    GTGGATTGCT TTATTAGAAA CAGTGAATAT AAAAAGCAAC CTGACTTCAG GTATCATCTT
    GATGATGAAG CTGATGAAAT GAAGTCTTTT CTGGGAATAT TTGATGGTAT TTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013852840.1
    CDS91..4527
    Translation

    Target ORF information:

    RefSeq Version XM_013997386.1
    Organism Sus scrofa(Pig)
    Definition Sus scrofa HFM1, ATP-dependent DNA helicase homolog (HFM1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_013997386.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCTGAGAT CACATGACTC CTTGTGTTCT TTGGATAATT TATTTTTTGA AAAAACAGAT 
    GAAGCTGAAA AATACCCAGA CAATGAAAAA TCATTAGATT GGTTTCTCCC ACCTGCTCCA
    TTGATTTCAG AAATTCCAGA TACTCAAGCA TTAGAGGAAG ACATGCAAAG TCATAAACTG
    TTAGGTCAGA AAAAGAGGTC AAAAATGTTA ACATCCAATT TAAAGATAAC TAATGAAGAC
    ACAAGTTATA CTTCACCAAC ACAAAAATTC CAGTGTGCTT TCCCTTCTAA TAAATACGAA
    GAAGGCGATC TAAATTTAGT AGGGGCAGGT AGCAATGACT TACATGTTGC TGGCAAGCTG
    ACTTATGGTT CTCCTCAGAA GTACAAAAGT CACATTGGCC CTGAGATAGC ACTTGAGAAA
    AATGTTCCAG ATGATGCGAA AGTAGTTAAT TTTGCATACG ATAAAGGAGA GAGCACATCG
    GCCTTCCGAA AAAGATTATT TAAAATATCT GATAGTATAC ATGGGAATGC TTGTTCTAAT
    GACATTGCAA ACTTGGACTC TGATATTGGC TCAGCGAAGA TTGCCCAAAT GGAAATGAGC
    GAAGGGAAAT CGTGGAAATG GAACAATAGT AATCAGAAAC CTCAATATTC AGAAATTAAC
    ATGTTTACAG CCAGTGATAC TTTTTCTGCT TCTAAAATCA GAGAAGGCAT ATTCAAAGCA
    CCATCTTTTT CAGTTGCATC CCAGCCTCAT GAAGTTCAAG GGATAATAGA AAATGGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATCCCG GCAAAATTTA GAAGTGTTTT CAAAGAATTC
    CCATATTTCA ACTATATCCA GTCTAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    GTTGTGGTTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAA TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGCAGTCA GCGTTTTGAT GACTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC GGGAGATACA GTCATGGATG ACCTGTTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCAACTGGT TCGACTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTT GTCAGCAGGA TGAAAACTGT GCAGTTTTTA
    TCTCATGCTG TGGAAAATCC CAGAAATATT ATTCCAATGA GATTTGTAGC TGTATCTGCA
    ACAATTCCAA ATGCTGAGGA TATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCCGCTATC
    TGTCTGAAAA TGGATGAGAA ACATAGACCA GTGAAGCTTC GGAAAGTGGT CCTTGGTTTT
    CCCTGCAGTA ATAACCAAAC TGAATTTAAG TTTGATTTAA CCCTCAACTA CAAAATTGCC
    AGTGTTGTAC AAACGTACTC CGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTACAAC AGGCTGCTTC CATTCTTGTG AAAGATGCCA AGTTTATTAT GACGGTGGAA
    CAGAAACAGA GGTTACATAA ATGTGCATAT TCCATAAGAG ATTCAAAATT GAGAGATCTC
    TTAATACACG GTGTTGCTTA TCATCATGCT GGCATGGAGC TGTCAGATAG AAAAGTAGTC
    GAAGGAGCTT TTACTGTTGG AGATCTACCA GTTCTTTTTA CTACTAGTAC CTTAGCTATG
    GGAGTAAATT TGCCTGCTCA TCTAGTAGTT ATCAAATCTA CAATGCATTA TGCTGGAGGG
    ATGTTTGAAG AGTACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCAAC TGCAGTTATT ATGACTCGGT TAAGTACAAA AGAGAAGTAT
    ATTCAGATGC TGGCTTGTAG TGACACTGTA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT GTTGCATACC ATCACAGATG TCAACATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTGTA TATCAGAGCC TTGAAGAATC CGTCTCACTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATTTG
    AATGATTTAT CATCTCTGGA CTTGATAAAG ATGGATGAAG ATATTAATTT CAAGCCAACT
    GAGGCAGGAA GATTGATGGC TTGGTACTAT GTTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATCAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG GATAAATGAA AAGAAAACAC TGAATACTTT GAACAAAGAT
    CCAAATCGGA TAACTATCAG ATTTCCAATG GAAGGAAAAA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCA TACAAGACTT TGCTTTGACA
    CAAGATACCT CAAAGATTTT CAGGAATGGC TCACGAATTA CGAGATGGTT ATCAGATTTT
    GTGGCTGTTC AAGAAAAGTT TGCTGTGCTT TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCTGCAT GTATCCAAAC AACTGGAAAA AATCGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AGCTTGAACT GATTTTAAAT AGACATCCCC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTGTCTGCC AAAATACGAA CTTGAAGTGG AACAGATTGC AAGATACAGT
    GATACCACGG CAGAAGTTTT AGTGACTGTT ATATTAAGAA ACTTCGAACC ACTGCAAACT
    AAAAGAACAG CACCAGATTC TCACTATGTT ACCTTAATCA TAGGCGATGC AGATAATCAG
    CTGATTTTTA AGCATAAAAT TATGGATTCT GTTTTGCTGA AATCTGGAAA TTGGGCTAAA
    AAGATCGATG TGAAGAGAGC TCTGAAATCT GAAGATCTTA GCATAAATCT CATTAGTTCT
    GAGTATGTTG GACTTGATAT TCAGCAGAAA TTTACAGTCT TTTACTTAGG ACCCAAGAGA
    TTTGGAAATC AAATAATTAT GCAAAAAAAA TCAGAAACAG AGATCTTCCA TTCTAAACGT
    TCAGATGGAT CTCCTGTAGC AGGTCCCATT AAAGGAATGA CTGCCTGCAG AAAGCCTGGG
    CACCGAGAGT GTAACCATCA CTGTAAAAAG AAACACACGT GTGGACATGA CTGCTGTAAA
    ACTGGAGTTG CCCAGAAGCC AGAAATGAAA GAGTCAACAA TTTCTTCTTA TTTATCTGAT
    TTAAGAAACA GGAATGCAGT TTCATCTCTT CCTCCAGTTA AACGTCTAAA GATGCAGATG
    AATAAATCTC AAAGTGTGGA CCTTAAAGAG TTTGGTTTTA CTCCACAACC TTCTCTTCCC
    AGTCTGTCAA GGTCAGAATG TTTAAACATA CCTAAATTGC CTGTAATGGA GCAGAGGGGT
    CAGCACGAGC TCTATGCAGA AGGTCAACAA CAAGAACCAC CGGGATATCA GGACAAAGGT
    TTTTCCAGAC AGACCTCTTC TACAACCTCA AATGAGAAAA CCCCTCAAAC AACACTGTTC
    AACAGAAATA TTAAGGCAAC TGGAAACCAG AATTTGACAG CTGTGGTTAA AAGTAATTCT
    TCCTATTCAG AAGTTTTGAA TGTGAACTTT GAATTGGGAA ATGAAGTTTG GGATGATTTT
    GATGATGAAA ACTTAATAGA AATTGCTAGC TTTTCAGCTG ATACTGAAAA GACAAAAGCA
    GGATTTGGAA ACACTTTGAG TTCAAGTGCC AGGGAAAATA AGCTACCCTT CCAAGAATCA
    AAAAGCAAAT TCCAAAGAAA AATGTCAAAC AGTTTTGTTT CATCACATGA GAAGCCAGAT
    ATTTATTTAC CAAATTCTGC TCTGTCCAAG ATCAGTGCAT CCTCCATGAC AAAATTACCT
    CAACCAGCTG GAAATGTCCC TTTCCAAGAA ATAAAACAAC AGAATCTGTC ACCAGAGATT
    GAAAAGCTAT GCTTTGCTCA CTCTGAAAAA ATATCAAGCT TTTCAAATTC TGTTACGCAA
    GTGGATTGCT TTATTAGAAA CAGTGAATAT AAAAAGCAAC CTGACTTCAG GTATCATCTT
    GATGATGAAG CTGATGAAAT GAAGTCTTTT CTGGGAATAT TTGATGGTAT TTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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