ORF » Species Summary » Sus scrofa » LOC100517289 cDNA ORF clone

LOC100517289 cDNA ORF clone, Sus scrofa(Pig)

The following LOC100517289 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100517289 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSe12980 XM_003482102.3
Latest version!		 Sus scrofa E3 ubiquitin-protein ligase RNF220 (LOC100517289), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OSe221470 XM_003482102.4
Latest version!		 Sus scrofa ring finger protein 220 (RNF220), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
OSe37592 XM_013988912.1
Latest version!		 Sus scrofa E3 ubiquitin-protein ligase RNF220 (LOC100517289), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    LOC100517289 ( XM_003482102.3 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_003482150.1 Sus scrofa E3 ubiquitin-protein ligase RNF220 (LOC100517289), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OSe12980
    Clone ID Related Accession (Same CDS sequence) XM_003482102.3
    Accession Version XM_003482102.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1701bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-10
    Organism Sus scrofa(Pig)
    Product E3 ubiquitin-protein ligase RNF220 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003535121.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 11, 2015 this sequence version replaced XM_003482102.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGACTTAC ACCGGGCAGC CTTCAAGATG GAGAACTCAT CCTACCTCCC CAACCCGCTG 
    GCATCCCCGG CACTGATGGT CCTGGCGTCC ACGGCCGAGG CCAGCCGTGA TGCTTCCATC 
    CCCTGCCAGC AGCCACGACC CTTCGGTGTG CCTGTCTCTG TAGACAAGGA CGTGCACATT 
    CCCTTCACCA ACGGTTCCTA CACCTTCGCC TCCATGTACC ATCGGCAAGG TGGGGTGCCG 
    GGCACCTTTG CCAACCGTGA TTTCCCCCCT TCTTTACTGC ACCTGCACCC CCAGTTTGCT 
    CCCCCCAATC TCGATTGCAC CCCGATCAGT ATGCTGAATC ACAGCGGAGT GGGGGCCTTC 
    CGGCCCTTTG CTTCCGCCGA GGACCGGGAG AGTTACCAGT CAGCCTTCAC GCCCGCCAAG 
    CGACTTAAAA ACTGCCACGA CACAGAGTCT CCCCACTTGC GCTTCTCAGA TGCAGATGGC 
    AAGGAATATG ACTTTGGGAC ACAGCTGCCA TCTAGCTCCC CCGGTTCACT CAAGGTTGAC 
    GACACTGGGA AGAAGATTTT TGCTGTCTCT GGCCTCATTT CGGACCGGGA AGCCTCATCT 
    AGCCCAGAGG ATCGGAATGA CAGATGTAAG AAAAAGGCAG CAGCATTGTT TGACAGCCAG 
    GCCCCTATTT GCCCCATCTG CCAGGTCCTG CTGCGGCCCA GCGAGCTGCA GGAACATATG 
    GAGCAAGAAC TGGAACAGCT GGCCCAGCTG CCCTCCAGCA AGAATTCCCT TCTCAAGGAT 
    GCCATGGCTC CAGGCACCCC GAAGTCCCTC TTGTTGTCTG CGTCCATCAA GAGGGAAGGA 
    GAGTCTCCAA CAGCATCGCC ACACTCATCT GCCACCGACG ACCTGCACCA CTCAGACAGA 
    TACCAGACCT TCCTGCGAGT GCGAGCCAAC CGGCAGACCC GACTGAATGC TCGGATTGGG 
    AAAATGAAAC GGAGGAAGCA GGATGAAGGG CAGAGGGAAG GCTCCTGCAT GGCGGAGGAG 
    GATGCCGTGG ACATCGAGCA TGAGAACAGC AACCGCTTTG AGGAGTATGA GTGGTGCGGG 
    CAGAAGCGGA TACGGGCCAC CACGCTCCTG GAAGGTGGTT TCCGAGGCTC TGGCTTCGTC 
    ATGTGCAGCG GCAAAGAGAA TCCGGACAGC GACGCTGACT TGGATGTGGA TGGGGACGAC 
    ACTCTGGAGT ACGGGAAGCC ACAATACACG GAGGCCGACG TCATCCCCTG CACAGGCGAG 
    GAGCCTGGTG AAGCCAAGGA GAGAGAGGCA CTCCGGGGTG CAGTCCTAAA TGGCGGCCCT 
    CCCAGCACAC GCATCACGCC TGAGTTCTCT AAGTGGGCCA GTGACGAGAT GCCATCTACC 
    AGCAACGGTG AGAGCAGCAA GCAGGAGGCC ATGCAGAAGA CCTGCAAGAA CAGCGACATC 
    GAGAAAATCA CCGAAGATTC AGCTGTGACC ACATTTGAGG CCCTGAAGGC TCGGGTCAGG 
    GAACTTGAAC GGCAGCTATC CCGTGGGGAC CGTTACAAAT GCCTCATCTG CATGGACTCC 
    TATTCGATGC CCCTAACGTC CATCCAGTGT TGGCACGTGC ACTGTGAGGA GTGCTGGCTG 
    CGGACCCTGG GTGCCAAGAA GCTCTGCCCT CAGTGCAACA CCATCACAGC GCCCGGAGAC 
    CTGCGGAGAA TCTACTTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003482150.1
    CDS376..2076
    Translation

    Target ORF information:

    RefSeq Version XM_003482102.3
    Organism Sus scrofa(Pig)
    Definition Sus scrofa E3 ubiquitin-protein ligase RNF220 (LOC100517289), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_003482102.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGACTTAC ACCGGGCAGC CTTCAAGATG GAGAACTCAT CCTACCTCCC CAACCCGCTG 
    GCATCCCCGG CACTGATGGT CCTGGCGTCC ACGGCCGAGG CCAGCCGTGA TGCTTCCATC 
    CCCTGCCAGC AGCCACGACC CTTCGGTGTG CCTGTCTCTG TAGACAAGGA CGTGCACATT 
    CCCTTCACCA ACGGTTCCTA CACCTTCGCC TCCATGTACC ATCGGCAAGG TGGGGTGCCG 
    GGCACCTTTG CCAACCGTGA TTTCCCCCCT TCTTTACTGC ACCTGCACCC CCAGTTTGCT 
    CCCCCCAATC TCGATTGCAC CCCGATCAGT ATGCTGAATC ACAGCGGAGT GGGGGCCTTC 
    CGGCCCTTTG CTTCCGCCGA GGACCGGGAG AGTTACCAGT CAGCCTTCAC GCCCGCCAAG 
    CGACTTAAAA ACTGCCACGA CACAGAGTCT CCCCACTTGC GCTTCTCAGA TGCAGATGGC 
    AAGGAATATG ACTTTGGGAC ACAGCTGCCA TCTAGCTCCC CCGGTTCACT CAAGGTTGAC 
    GACACTGGGA AGAAGATTTT TGCTGTCTCT GGCCTCATTT CGGACCGGGA AGCCTCATCT 
    AGCCCAGAGG ATCGGAATGA CAGATGTAAG AAAAAGGCAG CAGCATTGTT TGACAGCCAG 
    GCCCCTATTT GCCCCATCTG CCAGGTCCTG CTGCGGCCCA GCGAGCTGCA GGAACATATG 
    GAGCAAGAAC TGGAACAGCT GGCCCAGCTG CCCTCCAGCA AGAATTCCCT TCTCAAGGAT 
    GCCATGGCTC CAGGCACCCC GAAGTCCCTC TTGTTGTCTG CGTCCATCAA GAGGGAAGGA 
    GAGTCTCCAA CAGCATCGCC ACACTCATCT GCCACCGACG ACCTGCACCA CTCAGACAGA 
    TACCAGACCT TCCTGCGAGT GCGAGCCAAC CGGCAGACCC GACTGAATGC TCGGATTGGG 
    AAAATGAAAC GGAGGAAGCA GGATGAAGGG CAGAGGGAAG GCTCCTGCAT GGCGGAGGAG 
    GATGCCGTGG ACATCGAGCA TGAGAACAGC AACCGCTTTG AGGAGTATGA GTGGTGCGGG 
    CAGAAGCGGA TACGGGCCAC CACGCTCCTG GAAGGTGGTT TCCGAGGCTC TGGCTTCGTC 
    ATGTGCAGCG GCAAAGAGAA TCCGGACAGC GACGCTGACT TGGATGTGGA TGGGGACGAC 
    ACTCTGGAGT ACGGGAAGCC ACAATACACG GAGGCCGACG TCATCCCCTG CACAGGCGAG 
    GAGCCTGGTG AAGCCAAGGA GAGAGAGGCA CTCCGGGGTG CAGTCCTAAA TGGCGGCCCT 
    CCCAGCACAC GCATCACGCC TGAGTTCTCT AAGTGGGCCA GTGACGAGAT GCCATCTACC 
    AGCAACGGTG AGAGCAGCAA GCAGGAGGCC ATGCAGAAGA CCTGCAAGAA CAGCGACATC 
    GAGAAAATCA CCGAAGATTC AGCTGTGACC ACATTTGAGG CCCTGAAGGC TCGGGTCAGG 
    GAACTTGAAC GGCAGCTATC CCGTGGGGAC CGTTACAAAT GCCTCATCTG CATGGACTCC 
    TATTCGATGC CCCTAACGTC CATCCAGTGT TGGCACGTGC ACTGTGAGGA GTGCTGGCTG 
    CGGACCCTGG GTGCCAAGAA GCTCTGCCCT CAGTGCAACA CCATCACAGC GCCCGGAGAC 
    CTGCGGAGAA TCTACTTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LOC100517289 ( XM_003482102.4 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_003482150.2 Sus scrofa ring finger protein 220 (RNF220), mRNA.
    Price & Availability↑
    CloneID OSe221470
    Clone ID Related Accession (Same CDS sequence) XM_003482102.4
    Accession Version XM_003482102.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1701bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase RNF220
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010448.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_003482102.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGACTTAC ACCGGGCAGC CTTCAAGATG GAGAACTCAT CCTACCTCCC CAACCCGCTG 
    GCATCCCCGG CACTGATGGT CCTGGCGTCC ACGGCCGAGG CCAGCCGTGA TGCTTCCATC 
    CCCTGCCAGC AGCCACGACC CTTCGGTGTG CCTGTCTCTG TAGACAAGGA CGTGCACATT 
    CCCTTCACCA ACGGTTCCTA CACCTTCGCC TCCATGTACC ATCGGCAAGG TGGGGTGCCG 
    GGCACCTTTG CCAACCGTGA TTTCCCCCCT TCTTTACTGC ACCTGCACCC CCAGTTTGCT 
    CCCCCCAATC TCGATTGCAC CCCGATCAGT ATGCTGAATC ACAGCGGAGT GGGGGCCTTC 
    CGGCCCTTTG CTTCCGCCGA GGACCGGGAG AGTTACCAGT CAGCCTTCAC GCCCGCCAAG 
    CGACTTAAAA ACTGCCACGA CACAGAGTCT CCCCACTTGC GCTTCTCAGA TGCAGATGGC 
    AAGGAATATG ACTTTGGGAC ACAGCTGCCA TCTAGCTCCC CCGGTTCACT CAAGGTTGAC 
    GACACTGGGA AGAAGATTTT TGCTGTCTCT GGCCTCATTT CGGACCGGGA AGCCTCATCT 
    AGCCCAGAGG ATCGGAATGA CAGATGTAAG AAAAAGGCAG CAGCATTGTT TGACAGCCAG 
    GCCCCTATTT GCCCCATCTG CCAGGTCCTG CTGCGGCCCA GCGAGCTGCA GGAACATATG 
    GAGCAAGAAC TGGAACAGCT GGCCCAGCTG CCCTCCAGCA AGAATTCCCT TCTCAAGGAT 
    GCCATGGCTC CAGGCACCCC GAAGTCCCTC TTGTTGTCTG CGTCCATCAA GAGGGAAGGA 
    GAGTCTCCAA CAGCATCGCC ACACTCATCT GCCACCGACG ACCTGCACCA CTCAGACAGA 
    TACCAGACCT TCCTGCGAGT GCGAGCCAAC CGGCAGACCC GACTGAATGC TCGGATTGGG 
    AAAATGAAAC GGAGGAAGCA GGATGAAGGG CAGAGGGAAG GCTCCTGCAT GGCGGAGGAG 
    GATGCCGTGG ACATCGAGCA TGAGAACAGC AACCGCTTTG AGGAGTATGA GTGGTGCGGG 
    CAGAAGCGGA TACGGGCCAC CACGCTCCTG GAAGGTGGTT TCCGAGGCTC TGGCTTCGTC 
    ATGTGCAGCG GCAAAGAGAA TCCGGACAGC GACGCTGACT TGGATGTGGA TGGGGACGAC 
    ACTCTGGAGT ACGGGAAGCC ACAATACACG GAGGCCGACG TCATCCCCTG CACAGGCGAG 
    GAGCCTGGCG AAGCCAAGGA GAGAGAGGCA CTCCGGGGTG CAGTCCTAAA TGGCGGCCCT 
    CCCAGCACAC GCATCACGCC TGAGTTCTCT AAGTGGGCCA GTGACGAGAT GCCATCTACC 
    AGCAACGGTG AGAGCAGCAA GCAGGAGGCC ATGCAGAAGA CCTGCAAGAA CAGCGACATC 
    GAGAAAATCA CCGAAGATTC AGCTGTGACC ACATTTGAGG CCCTGAAGGC TCGGGTCAGG 
    GAACTTGAAC GGCAGCTATC CCGTNGGGAC CGTTACAAAT GCCTCATCTG CATGGACTCC 
    TATTCGATGC CCCTAACGTC CATCCAGTGT TGGCACGTGC ACTGTGAGGA GTGCTGGCTG 
    CGGACCCTGG GTGCCAAGAA GCTCTGCCCT CAGTGCAACA CCATCACAGC GCCCGGAGAC 
    CTGCGGAGAA TCTACTTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003482150.2
    CDS376..2076
    Translation

    Target ORF information:

    RefSeq Version XM_003482102.4
    Organism Sus scrofa(pig)
    Definition Sus scrofa ring finger protein 220 (RNF220), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_003482102.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGACTTAC ACCGGGCAGC CTTCAAGATG GAGAACTCAT CCTACCTCCC CAACCCGCTG 
    GCATCCCCGG CACTGATGGT CCTGGCGTCC ACGGCCGAGG CCAGCCGTGA TGCTTCCATC 
    CCCTGCCAGC AGCCACGACC CTTCGGTGTG CCTGTCTCTG TAGACAAGGA CGTGCACATT 
    CCCTTCACCA ACGGTTCCTA CACCTTCGCC TCCATGTACC ATCGGCAAGG TGGGGTGCCG 
    GGCACCTTTG CCAACCGTGA TTTCCCCCCT TCTTTACTGC ACCTGCACCC CCAGTTTGCT 
    CCCCCCAATC TCGATTGCAC CCCGATCAGT ATGCTGAATC ACAGCGGAGT GGGGGCCTTC 
    CGGCCCTTTG CTTCCGCCGA GGACCGGGAG AGTTACCAGT CAGCCTTCAC GCCCGCCAAG 
    CGACTTAAAA ACTGCCACGA CACAGAGTCT CCCCACTTGC GCTTCTCAGA TGCAGATGGC 
    AAGGAATATG ACTTTGGGAC ACAGCTGCCA TCTAGCTCCC CCGGTTCACT CAAGGTTGAC 
    GACACTGGGA AGAAGATTTT TGCTGTCTCT GGCCTCATTT CGGACCGGGA AGCCTCATCT 
    AGCCCAGAGG ATCGGAATGA CAGATGTAAG AAAAAGGCAG CAGCATTGTT TGACAGCCAG 
    GCCCCTATTT GCCCCATCTG CCAGGTCCTG CTGCGGCCCA GCGAGCTGCA GGAACATATG 
    GAGCAAGAAC TGGAACAGCT GGCCCAGCTG CCCTCCAGCA AGAATTCCCT TCTCAAGGAT 
    GCCATGGCTC CAGGCACCCC GAAGTCCCTC TTGTTGTCTG CGTCCATCAA GAGGGAAGGA 
    GAGTCTCCAA CAGCATCGCC ACACTCATCT GCCACCGACG ACCTGCACCA CTCAGACAGA 
    TACCAGACCT TCCTGCGAGT GCGAGCCAAC CGGCAGACCC GACTGAATGC TCGGATTGGG 
    AAAATGAAAC GGAGGAAGCA GGATGAAGGG CAGAGGGAAG GCTCCTGCAT GGCGGAGGAG 
    GATGCCGTGG ACATCGAGCA TGAGAACAGC AACCGCTTTG AGGAGTATGA GTGGTGCGGG 
    CAGAAGCGGA TACGGGCCAC CACGCTCCTG GAAGGTGGTT TCCGAGGCTC TGGCTTCGTC 
    ATGTGCAGCG GCAAAGAGAA TCCGGACAGC GACGCTGACT TGGATGTGGA TGGGGACGAC 
    ACTCTGGAGT ACGGGAAGCC ACAATACACG GAGGCCGACG TCATCCCCTG CACAGGCGAG 
    GAGCCTGGCG AAGCCAAGGA GAGAGAGGCA CTCCGGGGTG CAGTCCTAAA TGGCGGCCCT 
    CCCAGCACAC GCATCACGCC TGAGTTCTCT AAGTGGGCCA GTGACGAGAT GCCATCTACC 
    AGCAACGGTG AGAGCAGCAA GCAGGAGGCC ATGCAGAAGA CCTGCAAGAA CAGCGACATC 
    GAGAAAATCA CCGAAGATTC AGCTGTGACC ACATTTGAGG CCCTGAAGGC TCGGGTCAGG 
    GAACTTGAAC GGCAGCTATC CCGTNGGGAC CGTTACAAAT GCCTCATCTG CATGGACTCC 
    TATTCGATGC CCCTAACGTC CATCCAGTGT TGGCACGTGC ACTGTGAGGA GTGCTGGCTG 
    CGGACCCTGG GTGCCAAGAA GCTCTGCCCT CAGTGCAACA CCATCACAGC GCCCGGAGAC 
    CTGCGGAGAA TCTACTTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LOC100517289 ( XM_013988912.1 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_013844366.1 Sus scrofa E3 ubiquitin-protein ligase RNF220 (LOC100517289), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OSe37592
    Clone ID Related Accession (Same CDS sequence) XM_013988912.1
    Accession Version XM_013988912.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1779bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-10
    Organism Sus scrofa(Pig)
    Product E3 ubiquitin-protein ligase RNF220 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003535121.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGACTTAC ACCGGGCAGC CTTCAAGATG GAGAACTCAT CCTACCTCCC CAACCCGCTG 
    GCATCCCCGG CACTGATGGT CCTGGCGTCC ACGGCCGAGG CCAGCCGTGA TGCTTCCATC 
    CCCTGCCAGC AGCCACGACC CTTCGGTGTG CCTGTCTCTG TAGACAAGGA CGTGCACATT 
    CCCTTCACCA ACGGTTCCTA CACCTTCGCC TCCATGTACC ATCGGCAAGG TGGGGTGCCG 
    GGCACCTTTG CCAACCGTGA TTTCCCCCCT TCTTTACTGC ACCTGCACCC CCAGTTTGCT 
    CCCCCCAATC TCGATTGCAC CCCGATCAGT ATGCTGAATC ACAGCGGAGT GGGGGCCTTC 
    CGGCCCTTTG CTTCCGCCGA GGACCGGGAG AGTTACCAGT CAGCCTTCAC GCCCGCCAAG 
    CGACTTAAAA ACTGCCACGA CACAGAGTCT CCCCACTTGC GCTTCTCAGA TGCAGATGGC 
    AAGGAATATG ACTTTGGGAC ACAGCTGCCA TCTAGCTCCC CCGGTTCACT CAAGGTTGAC 
    GACACTGGGA AGAAGATTTT TGCTGTCTCT GGCCTCATTT CGGACCGGGA AGCCTCATCT 
    AGCCCAGAGG ATCGGAATGA CAGATGTAAG AAAAAGGCAG CAGCATTGTT TGACAGCCAG 
    GCCCCTATTT GCCCCATCTG CCAGGTCCTG CTGCGGCCCA GCGAGCTGCA GGAACATATG 
    GAGCAAGAAC TGGAACAGCT GGCCCAGCTG CCCTCCAGCA AGAATTCCCT TCTCAAGGAT 
    GCCATGGCTC CAGGCACCCC GAAGTCCCTC TTGTTGTCTG CGTCCATCAA GAGGGAAGGA 
    GAGTCTCCAA CAGCATCGCC ACACTCATCT GCCACCGACG ACCTGCACCA CTCAGACAGA 
    TACCAGACCT TCCTGCGAGT GCGAGCCAAC CGGCAGACCC GACTGAATGC TCGGATTGGG 
    AAAATGAAAC GGAGGAAGCA GGATGAAGGG CAGGTATGTC CCCTGTGCAG CCGCCCCCTG 
    GCAGGATCGG AGCAGGAGCT GAGTAGGCAT GTGGAGCGCT GCCTTTCTAA GAGGGAAGGC 
    TCCTGCATGG CGGAGGAGGA TGCCGTGGAC ATCGAGCATG AGAACAGCAA CCGCTTTGAG 
    GAGTATGAGT GGTGCGGGCA GAAGCGGATA CGGGCCACCA CGCTCCTGGA AGGTGGTTTC 
    CGAGGCTCTG GCTTCGTCAT GTGCAGCGGC AAAGAGAATC CGGACAGCGA CGCTGACTTG 
    GATGTGGATG GGGACGACAC TCTGGAGTAC GGGAAGCCAC AATACACGGA GGCCGACGTC 
    ATCCCCTGCA CAGGCGAGGA GCCTGGTGAA GCCAAGGAGA GAGAGGCACT CCGGGGTGCA 
    GTCCTAAATG GCGGCCCTCC CAGCACACGC ATCACGCCTG AGTTCTCTAA GTGGGCCAGT 
    GACGAGATGC CATCTACCAG CAACGGTGAG AGCAGCAAGC AGGAGGCCAT GCAGAAGACC 
    TGCAAGAACA GCGACATCGA GAAAATCACC GAAGATTCAG CTGTGACCAC ATTTGAGGCC 
    CTGAAGGCTC GGGTCAGGGA ACTTGAACGG CAGCTATCCC GTGGGGACCG TTACAAATGC 
    CTCATCTGCA TGGACTCCTA TTCGATGCCC CTAACGTCCA TCCAGTGTTG GCACGTGCAC 
    TGTGAGGAGT GCTGGCTGCG GACCCTGGGT GCCAAGAAGC TCTGCCCTCA GTGCAACACC 
    ATCACAGCGC CCGGAGACCT GCGGAGAATC TACTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013844366.1
    CDS376..2154
    Translation

    Target ORF information:

    RefSeq Version XM_013988912.1
    Organism Sus scrofa(Pig)
    Definition Sus scrofa E3 ubiquitin-protein ligase RNF220 (LOC100517289), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_013988912.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGACTTAC ACCGGGCAGC CTTCAAGATG GAGAACTCAT CCTACCTCCC CAACCCGCTG 
    GCATCCCCGG CACTGATGGT CCTGGCGTCC ACGGCCGAGG CCAGCCGTGA TGCTTCCATC 
    CCCTGCCAGC AGCCACGACC CTTCGGTGTG CCTGTCTCTG TAGACAAGGA CGTGCACATT 
    CCCTTCACCA ACGGTTCCTA CACCTTCGCC TCCATGTACC ATCGGCAAGG TGGGGTGCCG 
    GGCACCTTTG CCAACCGTGA TTTCCCCCCT TCTTTACTGC ACCTGCACCC CCAGTTTGCT 
    CCCCCCAATC TCGATTGCAC CCCGATCAGT ATGCTGAATC ACAGCGGAGT GGGGGCCTTC 
    CGGCCCTTTG CTTCCGCCGA GGACCGGGAG AGTTACCAGT CAGCCTTCAC GCCCGCCAAG 
    CGACTTAAAA ACTGCCACGA CACAGAGTCT CCCCACTTGC GCTTCTCAGA TGCAGATGGC 
    AAGGAATATG ACTTTGGGAC ACAGCTGCCA TCTAGCTCCC CCGGTTCACT CAAGGTTGAC 
    GACACTGGGA AGAAGATTTT TGCTGTCTCT GGCCTCATTT CGGACCGGGA AGCCTCATCT 
    AGCCCAGAGG ATCGGAATGA CAGATGTAAG AAAAAGGCAG CAGCATTGTT TGACAGCCAG 
    GCCCCTATTT GCCCCATCTG CCAGGTCCTG CTGCGGCCCA GCGAGCTGCA GGAACATATG 
    GAGCAAGAAC TGGAACAGCT GGCCCAGCTG CCCTCCAGCA AGAATTCCCT TCTCAAGGAT 
    GCCATGGCTC CAGGCACCCC GAAGTCCCTC TTGTTGTCTG CGTCCATCAA GAGGGAAGGA 
    GAGTCTCCAA CAGCATCGCC ACACTCATCT GCCACCGACG ACCTGCACCA CTCAGACAGA 
    TACCAGACCT TCCTGCGAGT GCGAGCCAAC CGGCAGACCC GACTGAATGC TCGGATTGGG 
    AAAATGAAAC GGAGGAAGCA GGATGAAGGG CAGGTATGTC CCCTGTGCAG CCGCCCCCTG 
    GCAGGATCGG AGCAGGAGCT GAGTAGGCAT GTGGAGCGCT GCCTTTCTAA GAGGGAAGGC 
    TCCTGCATGG CGGAGGAGGA TGCCGTGGAC ATCGAGCATG AGAACAGCAA CCGCTTTGAG 
    GAGTATGAGT GGTGCGGGCA GAAGCGGATA CGGGCCACCA CGCTCCTGGA AGGTGGTTTC 
    CGAGGCTCTG GCTTCGTCAT GTGCAGCGGC AAAGAGAATC CGGACAGCGA CGCTGACTTG 
    GATGTGGATG GGGACGACAC TCTGGAGTAC GGGAAGCCAC AATACACGGA GGCCGACGTC 
    ATCCCCTGCA CAGGCGAGGA GCCTGGTGAA GCCAAGGAGA GAGAGGCACT CCGGGGTGCA 
    GTCCTAAATG GCGGCCCTCC CAGCACACGC ATCACGCCTG AGTTCTCTAA GTGGGCCAGT 
    GACGAGATGC CATCTACCAG CAACGGTGAG AGCAGCAAGC AGGAGGCCAT GCAGAAGACC 
    TGCAAGAACA GCGACATCGA GAAAATCACC GAAGATTCAG CTGTGACCAC ATTTGAGGCC 
    CTGAAGGCTC GGGTCAGGGA ACTTGAACGG CAGCTATCCC GTGGGGACCG TTACAAATGC 
    CTCATCTGCA TGGACTCCTA TTCGATGCCC CTAACGTCCA TCCAGTGTTG GCACGTGCAC 
    TGTGAGGAGT GCTGGCTGCG GACCCTGGGT GCCAAGAAGC TCTGCCCTCA GTGCAACACC 
    ATCACAGCGC CCGGAGACCT GCGGAGAATC TACTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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