MYLK cDNA ORF clone, Sus scrofa(pig)

  • Gene

  • Clones

  • gRNAs

The following MYLK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYLK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSe236101 XM_001929078.6
Latest version!
Sus scrofa myosin light chain kinase (MYLK), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00
OSe42311 XM_005670159.2
Latest version!
Sus scrofa myosin light chain kinase (MYLK), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00
OSe236100 XM_021070241.1
Latest version!
Sus scrofa myosin light chain kinase (MYLK), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OSe236095 XM_021070231.1
Latest version!
Sus scrofa myosin light chain kinase (MYLK), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OSe236095 XM_021070234.1
Latest version!
Sus scrofa myosin light chain kinase (MYLK), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OSe236095 XM_021070233.1
Latest version!
Sus scrofa myosin light chain kinase (MYLK), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OSe236095 XM_021070235.1
Latest version!
Sus scrofa myosin light chain kinase (MYLK), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OSe236095 XM_021070232.1
Latest version!
Sus scrofa myosin light chain kinase (MYLK), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OSe236096 XM_021070237.1
Latest version!
Sus scrofa myosin light chain kinase (MYLK), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OSe236096 XM_021070236.1
Latest version!
Sus scrofa myosin light chain kinase (MYLK), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OSe236097 XM_021070238.1
Latest version!
Sus scrofa myosin light chain kinase (MYLK), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OSe236098 XM_021070239.1
Latest version!
Sus scrofa myosin light chain kinase (MYLK), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OSe236099 XM_021070240.1
Latest version!
Sus scrofa myosin light chain kinase (MYLK), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSe236101
    Clone ID Related Accession (Same CDS sequence) XM_001929078.6
    Accession Version XM_001929078.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 768bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product myosin light chain kinase, smooth muscle isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010455.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_001929078.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGGGTGACA ACGATAACGA GACCTTAGCC AACGTTACCT CAGCCACCTG GGACTTCGAC 
    GATGAGGCCT TCGATGAGAT CTCCGACGAC GCCAAGGATT TCATCAGCAA TTTACTGAAG
    AAAGATATGA AAAACCGCTT GGACTGCACC CAGTGCCTCC AGCACCCATG GCTAATGAAA
    GATACCAAGA ACATGGAGGC CAAGAAGCTC TCCAAGGACC GGATGAAAAA GTACATGGCA
    AGGAGGAAAT GGCAGAAAAC GGGCAATGCT GTGAGAGCCA TTGGAAGACT GTCCTCTATG
    GCAATGATCT CAGGGCTCAG TGGCAGGAAA TCCTCAACAG GGTCACCAAC CAGCCCGCTG
    AATGCAGAAA AATTAGAATC TGAAGAAGAT GTCTCCCAAG CTTTCCTTGA GGCCGTCGCT
    GAGGAAAAGC CCCATGTAAA ACCCTATTTT TCTAAGACCA TTCGTGATTT AGAAGTTGTG
    GAGGGAAGCG CTGCTAGATT TGACTGCAAG ATTGAAGGAT ACCCAGACCC TGAGGTTGTC
    TGGTTCAAAG ATGACCAGTC AATCAGGGAG TCCCGCCACT TCCAGATAGA CTACGATGAG
    GATGGGAACT GCTCTTTAAT CATTAGCGAT GTCTGCGGTG ATGATGATGC CAAGTACACC
    TGCAAGGCTG TCAACAGTCT TGGAGAAGCC ACCTGCACAG CAGAGCTCAT TGTGGAAACC
    ATGGAGGAAG GAGAAGGGGA AGGGGAAGAA GAAGAGGAGG AAGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001929113.6
    CDS227..994
    Translation

    Target ORF information:

    RefSeq Version XM_001929078.6
    Organism Sus scrofa(pig)
    Definition Sus scrofa myosin light chain kinase (MYLK), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001929078.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGGGTGACA ACGATAACGA GACCTTAGCC AACGTTACCT CAGCCACCTG GGACTTCGAC 
    GATGAGGCCT TCGATGAGAT CTCCGACGAC GCCAAGGATT TCATCAGCAA TTTACTGAAG
    AAAGATATGA AAAACCGCTT GGACTGCACC CAGTGCCTCC AGCACCCATG GCTAATGAAA
    GATACCAAGA ACATGGAGGC CAAGAAGCTC TCCAAGGACC GGATGAAAAA GTACATGGCA
    AGGAGGAAAT GGCAGAAAAC GGGCAATGCT GTGAGAGCCA TTGGAAGACT GTCCTCTATG
    GCAATGATCT CAGGGCTCAG TGGCAGGAAA TCCTCAACAG GGTCACCAAC CAGCCCGCTG
    AATGCAGAAA AATTAGAATC TGAAGAAGAT GTCTCCCAAG CTTTCCTTGA GGCCGTCGCT
    GAGGAAAAGC CCCATGTAAA ACCCTATTTT TCTAAGACCA TTCGTGATTT AGAAGTTGTG
    GAGGGAAGCG CTGCTAGATT TGACTGCAAG ATTGAAGGAT ACCCAGACCC TGAGGTTGTC
    TGGTTCAAAG ATGACCAGTC AATCAGGGAG TCCCGCCACT TCCAGATAGA CTACGATGAG
    GATGGGAACT GCTCTTTAAT CATTAGCGAT GTCTGCGGTG ATGATGATGC CAAGTACACC
    TGCAAGGCTG TCAACAGTCT TGGAGAAGCC ACCTGCACAG CAGAGCTCAT TGTGGAAACC
    ATGGAGGAAG GAGAAGGGGA AGGGGAAGAA GAAGAGGAGG AAGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe42311
    Clone ID Related Accession (Same CDS sequence) XM_005670159.2
    Accession Version XM_005670159.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1365bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-10
    Organism Sus scrofa(Pig)
    Product myosin light chain kinase, smooth muscle
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003611798.1) and transcript sequence (D89497.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 11, 2015 this sequence version replaced XM_005670159.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 226 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAAAGCCC GGAGGCCAAA GAGCTCTCTT CCTCCTGTGC TAGGAACAGA GAGTGATGCC 
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCGAAGG CAGCCATGCC CCCTCAGATC
    ATCCAGTTTC CCGAGGACCA AAAGGTGCGA GCGGGAGAGT CTGTGGAGCT GTTTGGTAAA
    GTGGCCGGTA CCCAGCCCAT CACCTGTACC TGGATGAAGT TCCGAAAGCA GATCCAGGAG
    AGTGAGCACA TCAAGGTGGA GAACAGTGAG AATGGCAGCA AGCTCACCAT CCGGGCCGCG
    CGCCAGGAGC ACTGTGGCTG CTACACTCTG CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG
    GCCCAGGTCA ACCTCACTGT TGTGGACAAG CCGGACCCCC CCGCAGGCAC GCCTTGTGCC
    TCTGACATCC GCAGCTCCTC GCTGACCCTG TCCTGGTATG GCTCCTCCTA CGATGGGGGC
    AGCGCCGTAC AGTCCTACAG CGTCGAGATC TGGGACTCAG TGGACAAGAC GTGGAAGGAA
    CTAGCCACAT GCCGCAGCAC CTCTTTTAAC GTCCAGGACC TGCTGTCTGA CCGGGAGTAT
    AAATTCCGTG TGCGTGCAAT CAACATCTAC GGAACCAGTG AGCCGAGCCA GGAGTCTGAA
    CTCACAGCGG TGGGAGAGAA ACCTGAGGAG CCCAAGGATG AAGTGGAGGT GTCAGATGAC
    GATGAGAAGG AGCCTGAGGT CGATTACCGG ACCGTCACGG TCAATACGGA ACAAAAAGTG
    TCTGACTTCT ACGACATTGA AGAGAGACTA GGATCTGGGA AATTTGGACA GGTCTTCCGA
    CTTGTAGAGA AGAAAACTGG AAAAATCTGG GCAGGGAAAT TCTTCAAGGC GTATTCAGCA
    AAAGAGAAAG AGAACATCCG GCAGGAGATC AGCATCATGA ACTGCCTCCA CCACCCCAAG
    CTGGTCCAGT GTGTGGACGC CTTCGAAGAG AAGGCCAACA TCGTCATGGT CCTGGAGATC
    GTGTCCGGGG GCGAGCTGTT CGAGCGCATC ATTGACGAGG ACTTTGAGCT GACGGAGCGC
    GAGTGCATCA AGTACATGCG GCAGATCTCG GAGGGGGTGG AGTACATCCA CAAGCAGGGC
    ATCGTCCACC TGGACCTCAA GCCTGAGAAC ATCATGTGTG TCAACAAGAC GGGCACCCGG
    ATCAAGCTCA TCGACTTCGG TCTGGCCCGG CGGCTGGAGA ATGCGGGGTC TCTGAAGGTC
    CTCTTTGGCA CCCCGGAGTT TGTGGCGCCT GAAGTGATCA ACTATGAGCC CATTGGCTAT
    GCCACGGACA TGTGGAGCAT CGGGGTCATC TGCTACATCC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005670216.2
    CDS175..1539
    Translation

    Target ORF information:

    RefSeq Version XM_005670159.2
    Organism Sus scrofa(Pig)
    Definition Sus scrofa myosin light chain kinase (MYLK), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005670159.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAAAGCCC GGAGGCCAAA GAGCTCTCTT CCTCCTGTGC TAGGAACAGA GAGTGATGCC 
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCGAAGG CAGCCATGCC CCCTCAGATC
    ATCCAGTTTC CCGAGGACCA AAAGGTGCGA GCGGGAGAGT CTGTGGAGCT GTTTGGTAAA
    GTGGCCGGTA CCCAGCCCAT CACCTGTACC TGGATGAAGT TCCGAAAGCA GATCCAGGAG
    AGTGAGCACA TCAAGGTGGA GAACAGTGAG AATGGCAGCA AGCTCACCAT CCGGGCCGCG
    CGCCAGGAGC ACTGTGGCTG CTACACTCTG CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG
    GCCCAGGTCA ACCTCACTGT TGTGGACAAG CCGGACCCCC CCGCAGGCAC GCCTTGTGCC
    TCTGACATCC GCAGCTCCTC GCTGACCCTG TCCTGGTATG GCTCCTCCTA CGATGGGGGC
    AGCGCCGTAC AGTCCTACAG CGTCGAGATC TGGGACTCAG TGGACAAGAC GTGGAAGGAA
    CTAGCCACAT GCCGCAGCAC CTCTTTTAAC GTCCAGGACC TGCTGTCTGA CCGGGAGTAT
    AAATTCCGTG TGCGTGCAAT CAACATCTAC GGAACCAGTG AGCCGAGCCA GGAGTCTGAA
    CTCACAGCGG TGGGAGAGAA ACCTGAGGAG CCCAAGGATG AAGTGGAGGT GTCAGATGAC
    GATGAGAAGG AGCCTGAGGT CGATTACCGG ACCGTCACGG TCAATACGGA ACAAAAAGTG
    TCTGACTTCT ACGACATTGA AGAGAGACTA GGATCTGGGA AATTTGGACA GGTCTTCCGA
    CTTGTAGAGA AGAAAACTGG AAAAATCTGG GCAGGGAAAT TCTTCAAGGC GTATTCAGCA
    AAAGAGAAAG AGAACATCCG GCAGGAGATC AGCATCATGA ACTGCCTCCA CCACCCCAAG
    CTGGTCCAGT GTGTGGACGC CTTCGAAGAG AAGGCCAACA TCGTCATGGT CCTGGAGATC
    GTGTCCGGGG GCGAGCTGTT CGAGCGCATC ATTGACGAGG ACTTTGAGCT GACGGAGCGC
    GAGTGCATCA AGTACATGCG GCAGATCTCG GAGGGGGTGG AGTACATCCA CAAGCAGGGC
    ATCGTCCACC TGGACCTCAA GCCTGAGAAC ATCATGTGTG TCAACAAGAC GGGCACCCGG
    ATCAAGCTCA TCGACTTCGG TCTGGCCCGG CGGCTGGAGA ATGCGGGGTC TCTGAAGGTC
    CTCTTTGGCA CCCCGGAGTT TGTGGCGCCT GAAGTGATCA ACTATGAGCC CATTGGCTAT
    GCCACGGACA TGTGGAGCAT CGGGGTCATC TGCTACATCC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe236100
    Clone ID Related Accession (Same CDS sequence) XM_021070241.1
    Accession Version XM_021070241.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2151bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product myosin light chain kinase, smooth muscle isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010455.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAAAGCCC GGAGGCCAAA GAGCTCTCTT CCTCCTGTGC TAGGAACAGA GAGTGATGCC 
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCGAAGG CAGCCATGCC CCCTCAGATC
    ATCCAGTTTC CCGAGGACCA AAAGGTGCGA GCGGGAGAGT CTGTGGAGCT GTTTGGTAAA
    GTGGCCGGTA CCCAGCCCAT CACCTGTACC TGGATGAAGT TCCGAAAGCA GATCCAGGAG
    AGTGAGCACA TCAAGGTGGA GAACAGTGAG AATGGCAGCA AGCTCACCAT CCGGGCCGCG
    CGCCAGGAGC ACTGTGGCTG CTACACTCTG CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG
    GCCCAGGTCA ACCTCACTGT TGTGGACAAG CCGGACCCCC CCGCAGGCAC GCCTTGTGCC
    TCTGACATCC GCAGCTCCTC GCTGACCCTG TCCTGGTATG GCTCCTCCTA CGATGGGGGC
    AGCGCCGTAC AGTCCTACAG CGTCGAGATC TGGGACTCAG TGGACAAGAC GTGGAAGGAA
    CTAGCCACAT GCCGCAGCAC CTCTTTTAAC GTCCAGGACC TGCTGTCTGA CCGGGAGTAT
    AAATTCCGTG TGCGTGCAAT CAACATCTAC GGAACCAGTG AGCCGAGCCA GGAGTCTGAA
    CTCACAGCGG TGGGAGAGAA ACCTGAGGAG CCCAAGGATG AAGTGGAGGT GTCAGATGAC
    GATGAGAAGG AGCCTGAGGT CGATTACCGG ACCGTCACGG TCAATACGGA ACAAAAAGTG
    TCTGACTTCT ACGACATTGA AGAGAGACTA GGATCTGGGA AATTTGGACA GGTCTTCCGA
    CTTGTAGAGA AGAAAACTGG AAAAATCTGG GCAGGGAAAT TCTTCAAGGC GTATTCAGCA
    AAAGAGAAAG AGAACATCCG GCAGGAGATC AGCATCATGA ACTGCCTCCA CCACCCCAAG
    CTGGTCCAGT GTGTGGACGC CTTCGAAGAG AAGGCCAACA TCGTCATGGT CCTGGAGATC
    GTGTCCGGGG GCGAGCTGTT CGAGCGCATC ATTGACGAGG ACTTTGAGCT GACGGAGCGC
    GAGTGCATCA AGTACATGCG GCAGATCTCG GAGGGGGTGG AGTACATCCA CAAGCAGGGC
    ATCGTCCACC TGGACCTCAA GCCTGAGAAC ATCATGTGTG TCAACAAGAC GGGCACCCGG
    ATCAAGCTCA TCGACTTCGG TCTGGCCCGG CGGCTGGAGA ATGCGGGGTC TCTGAAGGTC
    CTCTTTGGCA CCCCGGAGTT TGTGGCGCCT GAAGTGATCA ACTATGAGCC CATTGGCTAT
    GCCACGGACA TGTGGAGCAT CGGGGTCATC TGCTACATCC TGGTCAGTGG ACTGTCCCCC
    TTTATGGGTG ACAACGATAA CGAGACCTTA GCCAACGTTA CCTCAGCCAC CTGGGACTTC
    GACGATGAGG CCTTCGATGA GATCTCCGAC GACGCCAAGG ATTTCATCAG CAATTTACTG
    AAGAAAGATA TGAAAAACCG CTTGGACTGC ACCCAGTGCC TCCAGCACCC ATGGCTAATG
    AAAGATACCA AGAACATGGA GGCCAAGAAG CTCTCCAAGG ACCGGATGAA AAAGTACATG
    GCAAGGAGGA AATGGCAGAA AACGGGCAAT GCTGTGAGAG CCATTGGAAG ACTGTCCTCT
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG
    CTGAATGCAG AAAAATTAGA ATCTGAAGAA GATGTCTCCC AAGCTTTCCT TGAGGCCGTC
    GCTGAGGAAA AGCCCCATGT AAAACCCTAT TTTTCTAAGA CCATTCGTGA TTTAGAAGTT
    GTGGAGGGAA GCGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCTGAGGTT
    GTCTGGTTCA AAGATGACCA GTCAATCAGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT
    GAGGATGGGA ACTGCTCTTT AATCATTAGC GATGTCTGCG GTGATGATGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAACAG TCTTGGAGAA GCCACCTGCA CAGCAGAGCT CATTGTGGAA
    ACCATGGAGG AAGGAGAAGG GGAAGGGGAA GAAGAAGAGG AGGAAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020925900.1
    CDS191..2341
    Translation

    Target ORF information:

    RefSeq Version XM_021070241.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa myosin light chain kinase (MYLK), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021070241.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAAAGCCC GGAGGCCAAA GAGCTCTCTT CCTCCTGTGC TAGGAACAGA GAGTGATGCC 
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCGAAGG CAGCCATGCC CCCTCAGATC
    ATCCAGTTTC CCGAGGACCA AAAGGTGCGA GCGGGAGAGT CTGTGGAGCT GTTTGGTAAA
    GTGGCCGGTA CCCAGCCCAT CACCTGTACC TGGATGAAGT TCCGAAAGCA GATCCAGGAG
    AGTGAGCACA TCAAGGTGGA GAACAGTGAG AATGGCAGCA AGCTCACCAT CCGGGCCGCG
    CGCCAGGAGC ACTGTGGCTG CTACACTCTG CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG
    GCCCAGGTCA ACCTCACTGT TGTGGACAAG CCGGACCCCC CCGCAGGCAC GCCTTGTGCC
    TCTGACATCC GCAGCTCCTC GCTGACCCTG TCCTGGTATG GCTCCTCCTA CGATGGGGGC
    AGCGCCGTAC AGTCCTACAG CGTCGAGATC TGGGACTCAG TGGACAAGAC GTGGAAGGAA
    CTAGCCACAT GCCGCAGCAC CTCTTTTAAC GTCCAGGACC TGCTGTCTGA CCGGGAGTAT
    AAATTCCGTG TGCGTGCAAT CAACATCTAC GGAACCAGTG AGCCGAGCCA GGAGTCTGAA
    CTCACAGCGG TGGGAGAGAA ACCTGAGGAG CCCAAGGATG AAGTGGAGGT GTCAGATGAC
    GATGAGAAGG AGCCTGAGGT CGATTACCGG ACCGTCACGG TCAATACGGA ACAAAAAGTG
    TCTGACTTCT ACGACATTGA AGAGAGACTA GGATCTGGGA AATTTGGACA GGTCTTCCGA
    CTTGTAGAGA AGAAAACTGG AAAAATCTGG GCAGGGAAAT TCTTCAAGGC GTATTCAGCA
    AAAGAGAAAG AGAACATCCG GCAGGAGATC AGCATCATGA ACTGCCTCCA CCACCCCAAG
    CTGGTCCAGT GTGTGGACGC CTTCGAAGAG AAGGCCAACA TCGTCATGGT CCTGGAGATC
    GTGTCCGGGG GCGAGCTGTT CGAGCGCATC ATTGACGAGG ACTTTGAGCT GACGGAGCGC
    GAGTGCATCA AGTACATGCG GCAGATCTCG GAGGGGGTGG AGTACATCCA CAAGCAGGGC
    ATCGTCCACC TGGACCTCAA GCCTGAGAAC ATCATGTGTG TCAACAAGAC GGGCACCCGG
    ATCAAGCTCA TCGACTTCGG TCTGGCCCGG CGGCTGGAGA ATGCGGGGTC TCTGAAGGTC
    CTCTTTGGCA CCCCGGAGTT TGTGGCGCCT GAAGTGATCA ACTATGAGCC CATTGGCTAT
    GCCACGGACA TGTGGAGCAT CGGGGTCATC TGCTACATCC TGGTCAGTGG ACTGTCCCCC
    TTTATGGGTG ACAACGATAA CGAGACCTTA GCCAACGTTA CCTCAGCCAC CTGGGACTTC
    GACGATGAGG CCTTCGATGA GATCTCCGAC GACGCCAAGG ATTTCATCAG CAATTTACTG
    AAGAAAGATA TGAAAAACCG CTTGGACTGC ACCCAGTGCC TCCAGCACCC ATGGCTAATG
    AAAGATACCA AGAACATGGA GGCCAAGAAG CTCTCCAAGG ACCGGATGAA AAAGTACATG
    GCAAGGAGGA AATGGCAGAA AACGGGCAAT GCTGTGAGAG CCATTGGAAG ACTGTCCTCT
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG
    CTGAATGCAG AAAAATTAGA ATCTGAAGAA GATGTCTCCC AAGCTTTCCT TGAGGCCGTC
    GCTGAGGAAA AGCCCCATGT AAAACCCTAT TTTTCTAAGA CCATTCGTGA TTTAGAAGTT
    GTGGAGGGAA GCGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCTGAGGTT
    GTCTGGTTCA AAGATGACCA GTCAATCAGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT
    GAGGATGGGA ACTGCTCTTT AATCATTAGC GATGTCTGCG GTGATGATGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAACAG TCTTGGAGAA GCCACCTGCA CAGCAGAGCT CATTGTGGAA
    ACCATGGAGG AAGGAGAAGG GGAAGGGGAA GAAGAAGAGG AGGAAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe236095
    Clone ID Related Accession (Same CDS sequence) XM_021070232.1 , XM_021070231.1 , XM_021070234.1 , XM_021070235.1 , XM_021070233.1
    Accession Version XM_021070232.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5946bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product myosin light chain kinase, smooth muscle isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010455.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGATCACT CGAGAGTCGG CTCCACGCCC CTGACCGAGG CCCCTGCTTT CATTTTGCCC 
    CCTCGGAACC TTTGCATCAA GGAAGGAGCC ACTGCCAAGT TTGAAGGGAG GGTCCGGGGT
    TACCCAGAGC CCCAGGTGAC ATGGCACAGA AACGGACAGC CCATCACCAG TGGAGGTCGC
    TTCCTGCTGG ACTGTGGCAT CCGGGGGACC TTCAGTCTTG TGATTCATTC TGTCTGTGAG
    GAGGACAAGG GAAAGTATAC CTGTGAAGCC ACCAATGGTA GTGGCGCCCG CCAGGTGACA
    GTGGAGTTGA CGGTAGAAGG GGGCTTTGTG AAGAAGCATG GCCAGCCTGT TGTTTCCAAA
    GCCTTGGGGG ACAGATTTGC AGCCCCTGCA GTCGAGACCC GTCCTAGCAT CTGGGGAGAG
    TGCCCACCAA AGTTTGCCAC CAAGCTGGGC CGAGCGGTGG TCAAAGAAGG ACAGATGGGG
    CGATTCTCCT GCAAGATCAC TGGCCGGCCC CAACCGCAGG TCACCTGGCT CAAGGGAGAT
    GTTCCCCTGC AGCCCAGTGC CCGTGTGACC ATGTCTGAGA AGAATGGGGT GCAAGTGCTG
    GAAATCCACG AAGTCAACCG AGACGACGTG GGGGTGTACA CGTGCATGGT GGTGAATGGG
    TCGGGGAAGG CCTCCATGTC AGCTGAGCTC TCCATCCAAG GTTTGGACAA TACCAATAGG
    TCGCTTGTGA GAGGAACAAA GGCGGCCAGT TCAGACATCA GGACAGAAGC GACCAATGGA
    CTCTCACAGG GGCCAAAGCT GGATGGCCTG GAGGCTGCAG CGGAGAGTAA GAACTGCTCC
    AGCGCCCAGA GGGGTGGCTC CCCGACCTGG GCTTCAGGGA GCCAGCCTCA GCCTCCGAGG
    GAATCCAAGC TGGAGCCGTC AGGGGGCTCA GACAGAAAAG CCCTGAGGAG CCCCATCCTG
    CAGAAGACTT CCAGTGCCAT CACCCTGCAG CCTGCAAAAG TCCAGTTGGA ACCAAGGGCT
    CCAGTCTCAG GCACCCTCTT GCCTTCCAGA GAAGAGAGAG AGCGGCCAGC TGCTCCACCT
    GCCGCCACCC TGCCCTCCAG ACAGTCTGGC ACAGGAGGCC AAGAAGCTGT GAGCAAGGCT
    GCTACCAAGC AGGTTGCCAT GGAGAGCCGA AGGGCTGCCA CAGTCCCCAG ATTCGAGAGC
    AAGCCCCAAA GCCAGGAGGT CAGTGAAGAT CAGCCAGTCA AGTTCAAGTG TGAAGTTTCA
    GGGGTTCCAA AGCCCGAAGT GACCTGGTTC CTGGACGGTG CCCCTGTGAA GAGGCGAGAA
    GGCACCTTTG AGGTTTATGA AGCCGGAGGA TCCCACTACC TCTGCCTGCC AAGAGCCCGG
    GCCAGGGACA GCGGGAACTA CAGCTGCACA GCCACCAACA TCCGAGGCCA GGTGTCCTGT
    ACCTGGACCC TCCTGGTGAA AAGGCTGGCC GTGACAGAGG CGCCCCCGTC CTTCTCCAGT
    GTCCTGAAGG ACTGTGCTGT GGTTGAGGGC CAGGACTTCG TGCTGCAGTG CTCCGTGCAG
    GGGACCCCGC TGCCCCAGAT CACTTGGCTG CTCAATGGGC AGCCCATCCA GTACGCCCGC
    TCCACCTGCG AGGCCGGCGT TGCCAAGCTC CACATCCAGG ATGCCCTCCC GGAGGACGAC
    GGCACCTATA CCTGTCTGGC CGAGAACACC TTGGGGCGGG TGTCCTGCAG CGCCCGGGTC
    ACTGTCCATG AAAAGAAGAG TGATGGGAAG AGTGAGTACC TCCTGCCCGC TGCCCCCAGC
    AAGCCCATGG CACCCATCTT CCTGCAGGGT CTCTCTGATC TCAGCGTCAT GGATGGGAGC
    CAGGTCACCA TGACAGTCCA GGTGTCAGGG AACCCACCCC CTGAGGTCAT CTGGCTTCAC
    GATGGGAATG AGATCCAGGA GTCGGAGGAC TTCCACTTTG AGCAGAGGGG CACTCGGCAC
    AGCCTCTGTA TCCAGGAAGT GTTCCCAGAA GACACAGGCA CATACACCTG TGAAGCCTGG
    AACAGCGCAG GAGAGGTCCG CACCCAGGCC GTGCTCACGG TGCAAGAGCC TCAGGACGGC
    ACCCAGCCTT GGTTCATCAG CAAGCCCCGC TCTGTGACAG CCTGCCTGGG CCAGAGTGTC
    CTCATCTCCT GTGCCATAGC TGGGGACCCC TTCCCTACTG TGCACTGGCT CCGAGACGGC
    AAAGCCCTCT CCAAAGACAC TGGCCACTTC GAGGTGCTGC AGAATGAGGA CGTGTTCACC
    CTGGTTCTCA AGAACGTGCA GCCCTGGCAC GCCGGCCAGT ATGAGATCCT GCTCAAGAAC
    CGGGTTGGCG AGTGCAGCTG CCAGGTGTCC CTGGTGCTAC AGAGCAGCCC TGCCAGAGCC
    CCCCTGCGGG GGAGGGAGCC TGCCAGCTGT GAGGGCCAAG GAGCTGGTGC TGATGGTGGT
    GGTGCTGGCA GAGACGGCTA CGGGAGCCTG AGGCCCGGCT GGCCAGCAAG AGGGCAGGGT
    GAGCCCGAGG ACGAAGACGG CGAGGACGTG CGCGGGGTGC TCAAGAGGCG CGTGGAGACG
    CGGCAGCACA CCGAGGAGGC TATCCGCCAG CAGGAGGTGG AGCAGCTGGA CTTCCGCGAC
    CTCCTGGGCA AGAAGGTGAG CACCAAGACC TTGTCTGAGG AGGACCTGAA GGAGATCCCT
    GCCGAGCAGA TGGATTTCCG CGCCAATCTG CAGCGGCAAG TCAAGCCGAA GACTGTGTCC
    GAGGAAGAGA GGAAGGTGCA TAGCCCCCAG CAGGTGGACT TCCGCTCGGT CCTGGCCAAG
    AAGGGGACGC CCAAGACCCC CGTACCTGAG AAGCTGCCCC CTCCAAAACC TGCCACCCCA
    GATTTTCGCT CGGTGTTGGG CAGTAAGAAA AAATTACCAG CGGAGAATGG TAGCAGCAAT
    GCTGAGCCTT TGAATGTCAA GGCAGCAGAA GGTCCCAAGC CTGTGGGCAA TGCCCAGCCT
    TCAGGCTTTC TCAAACCTAT GGGCAATGCC AAGCTGGCCG AGACCTCCAA ACACTTGAGT
    AATGCCAAGC CAGCCGAGCT CCCCAAACCT GTGGGCAACG CCAAGCCAGC CGAGATCCCC
    AAACCCGTGG GCAACACCAG GCCAGCTGAG ACCCCCAAAA CCGTGGGCAA TGCCAAGCCA
    ACTGAGACCT TCAAACCCAT GGCCAATACC AAGCCAGCTG AGACCCTAAA GCCTGTGGGC
    AATGCCAAGC CAGCTGAGAC CCTAAAGCCT GTGGGCAATG CCAAGCCAGC TGAGACCCTA
    AAGCCCGTGG GCAATGCCAA GCCAGCTGAG ACCCCCAAGC CCTTGGGTAA TACCAAGCCA
    GCCGAGCCCC CTAAACCAGC TGGGAAAGAA GAGCTTAAGA AGGAGGTTAA GAATGATGTG
    AACTACAAGA GGGGCTCTGC AGGGGCCACA GATAATGAAA AAAGCCCGGA GAGCCAAGGG
    TCAGCCCCAA CCTTCAAGGA AAAGCTACGT GATCTCCGTG TGGCAGAGGG TGAGAAGCTG
    CTGCTCCAGT GCCAGGTATC CTCCGACCCC CCGGCCACCA TCACCTGGAC ACTGAATGGG
    AAGACACTCA AGACCACCAA GTTCATTGTC CTCTCCCAAG AAGGCTCACT GTGCTCCATC
    TCCATCGAGA AGGCCTTGCC CGAGGACAGA GGTTTGTACA AGTGTGTGGC CAAGAACAGC
    GCCGGCCAGG CTGAGTGCTC CTGCCAAGTC ACCGTGGACG ATGCCCCCAC CGGAGAGAAT
    GCCAAGGCCC CGGAGATGAA AGCCCGGAGG CCAAAGAGCT CTCTTCCTCC TGTGCTAGGA
    ACAGAGAGTG ATGCCACTGT GAAAAAGAAA CCTGCCCCCA AGACACCTCC GAAGGCAGCC
    ATGCCCCCTC AGATCATCCA GTTTCCCGAG GACCAAAAGG TGCGAGCGGG AGAGTCTGTG
    GAGCTGTTTG GTAAAGTGGC CGGTACCCAG CCCATCACCT GTACCTGGAT GAAGTTCCGA
    AAGCAGATCC AGGAGAGTGA GCACATCAAG GTGGAGAACA GTGAGAATGG CAGCAAGCTC
    ACCATCCGGG CCGCGCGCCA GGAGCACTGT GGCTGCTACA CTCTGCTGGT GGAGAACAAG
    CTGGGCAGCA GGCAGGCCCA GGTCAACCTC ACTGTTGTGG ACAAGCCGGA CCCCCCCGCA
    GGCACGCCTT GTGCCTCTGA CATCCGCAGC TCCTCGCTGA CCCTGTCCTG GTATGGCTCC
    TCCTACGATG GGGGCAGCGC CGTACAGTCC TACAGCGTCG AGATCTGGGA CTCAGTGGAC
    AAGACGTGGA AGGAACTAGC CACATGCCGC AGCACCTCTT TTAACGTCCA GGACCTGCTG
    TCTGACCGGG AGTATAAATT CCGTGTGCGT GCAATCAACA TCTACGGAAC CAGTGAGCCG
    AGCCAGGAGT CTGAACTCAC AGCGGTGGGA GAGAAACCTG AGGAGCCCAA GGATGAAGTG
    GAGGTGTCAG ATGACGATGA GAAGGAGCCT GAGGTCGATT ACCGGACCGT CACGGTCAAT
    ACGGAACAAA AAGTGTCTGA CTTCTACGAC ATTGAAGAGA GACTAGGATC TGGGAAATTT
    GGACAGGTCT TCCGACTTGT AGAGAAGAAA ACTGGAAAAA TCTGGGCAGG GAAATTCTTC
    AAGGCGTATT CAGCAAAAGA GAAAGAGAAC ATCCGGCAGG AGATCAGCAT CATGAACTGC
    CTCCACCACC CCAAGCTGGT CCAGTGTGTG GACGCCTTCG AAGAGAAGGC CAACATCGTC
    ATGGTCCTGG AGATCGTGTC CGGGGGCGAG CTGTTCGAGC GCATCATTGA CGAGGACTTT
    GAGCTGACGG AGCGCGAGTG CATCAAGTAC ATGCGGCAGA TCTCGGAGGG GGTGGAGTAC
    ATCCACAAGC AGGGCATCGT CCACCTGGAC CTCAAGCCTG AGAACATCAT GTGTGTCAAC
    AAGACGGGCA CCCGGATCAA GCTCATCGAC TTCGGTCTGG CCCGGCGGCT GGAGAATGCG
    GGGTCTCTGA AGGTCCTCTT TGGCACCCCG GAGTTTGTGG CGCCTGAAGT GATCAACTAT
    GAGCCCATTG GCTATGCCAC GGACATGTGG AGCATCGGGG TCATCTGCTA CATCCTGGTC
    AGTGGACTGT CCCCCTTTAT GGGTGACAAC GATAACGAGA CCTTAGCCAA CGTTACCTCA
    GCCACCTGGG ACTTCGACGA TGAGGCCTTC GATGAGATCT CCGACGACGC CAAGGATTTC
    ATCAGCAATT TACTGAAGAA AGATATGAAA AACCGCTTGG ACTGCACCCA GTGCCTCCAG
    CACCCATGGC TAATGAAAGA TACCAAGAAC ATGGAGGCCA AGAAGCTCTC CAAGGACCGG
    ATGAAAAAGT ACATGGCAAG GAGGAAATGG CAGAAAACGG GCAATGCTGT GAGAGCCATT
    GGAAGACTGT CCTCTATGGC AATGATCTCA GGGCTCAGTG GCAGGAAATC CTCAACAGGG
    TCACCAACCA GCCCGCTGAA TGCAGAAAAA TTAGAATCTG AAGAAGATGT CTCCCAAGCT
    TTCCTTGAGG CCGTCGCTGA GGAAAAGCCC CATGTAAAAC CCTATTTTTC TAAGACCATT
    CGTGATTTAG AAGTTGTGGA GGGAAGCGCT GCTAGATTTG ACTGCAAGAT TGAAGGATAC
    CCAGACCCTG AGGTTGTCTG GTTCAAAGAT GACCAGTCAA TCAGGGAGTC CCGCCACTTC
    CAGATAGACT ACGATGAGGA TGGGAACTGC TCTTTAATCA TTAGCGATGT CTGCGGTGAT
    GATGATGCCA AGTACACCTG CAAGGCTGTC AACAGTCTTG GAGAAGCCAC CTGCACAGCA
    GAGCTCATTG TGGAAACCAT GGAGGAAGGA GAAGGGGAAG GGGAAGAAGA AGAGGAGGAA
    GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020925891.1
    CDS315..6260
    Translation

    Target ORF information:

    RefSeq Version XM_021070232.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa myosin light chain kinase (MYLK), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021070232.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGATCACT CGAGAGTCGG CTCCACGCCC CTGACCGAGG CCCCTGCTTT CATTTTGCCC 
    CCTCGGAACC TTTGCATCAA GGAAGGAGCC ACTGCCAAGT TTGAAGGGAG GGTCCGGGGT
    TACCCAGAGC CCCAGGTGAC ATGGCACAGA AACGGACAGC CCATCACCAG TGGAGGTCGC
    TTCCTGCTGG ACTGTGGCAT CCGGGGGACC TTCAGTCTTG TGATTCATTC TGTCTGTGAG
    GAGGACAAGG GAAAGTATAC CTGTGAAGCC ACCAATGGTA GTGGCGCCCG CCAGGTGACA
    GTGGAGTTGA CGGTAGAAGG GGGCTTTGTG AAGAAGCATG GCCAGCCTGT TGTTTCCAAA
    GCCTTGGGGG ACAGATTTGC AGCCCCTGCA GTCGAGACCC GTCCTAGCAT CTGGGGAGAG
    TGCCCACCAA AGTTTGCCAC CAAGCTGGGC CGAGCGGTGG TCAAAGAAGG ACAGATGGGG
    CGATTCTCCT GCAAGATCAC TGGCCGGCCC CAACCGCAGG TCACCTGGCT CAAGGGAGAT
    GTTCCCCTGC AGCCCAGTGC CCGTGTGACC ATGTCTGAGA AGAATGGGGT GCAAGTGCTG
    GAAATCCACG AAGTCAACCG AGACGACGTG GGGGTGTACA CGTGCATGGT GGTGAATGGG
    TCGGGGAAGG CCTCCATGTC AGCTGAGCTC TCCATCCAAG GTTTGGACAA TACCAATAGG
    TCGCTTGTGA GAGGAACAAA GGCGGCCAGT TCAGACATCA GGACAGAAGC GACCAATGGA
    CTCTCACAGG GGCCAAAGCT GGATGGCCTG GAGGCTGCAG CGGAGAGTAA GAACTGCTCC
    AGCGCCCAGA GGGGTGGCTC CCCGACCTGG GCTTCAGGGA GCCAGCCTCA GCCTCCGAGG
    GAATCCAAGC TGGAGCCGTC AGGGGGCTCA GACAGAAAAG CCCTGAGGAG CCCCATCCTG
    CAGAAGACTT CCAGTGCCAT CACCCTGCAG CCTGCAAAAG TCCAGTTGGA ACCAAGGGCT
    CCAGTCTCAG GCACCCTCTT GCCTTCCAGA GAAGAGAGAG AGCGGCCAGC TGCTCCACCT
    GCCGCCACCC TGCCCTCCAG ACAGTCTGGC ACAGGAGGCC AAGAAGCTGT GAGCAAGGCT
    GCTACCAAGC AGGTTGCCAT GGAGAGCCGA AGGGCTGCCA CAGTCCCCAG ATTCGAGAGC
    AAGCCCCAAA GCCAGGAGGT CAGTGAAGAT CAGCCAGTCA AGTTCAAGTG TGAAGTTTCA
    GGGGTTCCAA AGCCCGAAGT GACCTGGTTC CTGGACGGTG CCCCTGTGAA GAGGCGAGAA
    GGCACCTTTG AGGTTTATGA AGCCGGAGGA TCCCACTACC TCTGCCTGCC AAGAGCCCGG
    GCCAGGGACA GCGGGAACTA CAGCTGCACA GCCACCAACA TCCGAGGCCA GGTGTCCTGT
    ACCTGGACCC TCCTGGTGAA AAGGCTGGCC GTGACAGAGG CGCCCCCGTC CTTCTCCAGT
    GTCCTGAAGG ACTGTGCTGT GGTTGAGGGC CAGGACTTCG TGCTGCAGTG CTCCGTGCAG
    GGGACCCCGC TGCCCCAGAT CACTTGGCTG CTCAATGGGC AGCCCATCCA GTACGCCCGC
    TCCACCTGCG AGGCCGGCGT TGCCAAGCTC CACATCCAGG ATGCCCTCCC GGAGGACGAC
    GGCACCTATA CCTGTCTGGC CGAGAACACC TTGGGGCGGG TGTCCTGCAG CGCCCGGGTC
    ACTGTCCATG AAAAGAAGAG TGATGGGAAG AGTGAGTACC TCCTGCCCGC TGCCCCCAGC
    AAGCCCATGG CACCCATCTT CCTGCAGGGT CTCTCTGATC TCAGCGTCAT GGATGGGAGC
    CAGGTCACCA TGACAGTCCA GGTGTCAGGG AACCCACCCC CTGAGGTCAT CTGGCTTCAC
    GATGGGAATG AGATCCAGGA GTCGGAGGAC TTCCACTTTG AGCAGAGGGG CACTCGGCAC
    AGCCTCTGTA TCCAGGAAGT GTTCCCAGAA GACACAGGCA CATACACCTG TGAAGCCTGG
    AACAGCGCAG GAGAGGTCCG CACCCAGGCC GTGCTCACGG TGCAAGAGCC TCAGGACGGC
    ACCCAGCCTT GGTTCATCAG CAAGCCCCGC TCTGTGACAG CCTGCCTGGG CCAGAGTGTC
    CTCATCTCCT GTGCCATAGC TGGGGACCCC TTCCCTACTG TGCACTGGCT CCGAGACGGC
    AAAGCCCTCT CCAAAGACAC TGGCCACTTC GAGGTGCTGC AGAATGAGGA CGTGTTCACC
    CTGGTTCTCA AGAACGTGCA GCCCTGGCAC GCCGGCCAGT ATGAGATCCT GCTCAAGAAC
    CGGGTTGGCG AGTGCAGCTG CCAGGTGTCC CTGGTGCTAC AGAGCAGCCC TGCCAGAGCC
    CCCCTGCGGG GGAGGGAGCC TGCCAGCTGT GAGGGCCAAG GAGCTGGTGC TGATGGTGGT
    GGTGCTGGCA GAGACGGCTA CGGGAGCCTG AGGCCCGGCT GGCCAGCAAG AGGGCAGGGT
    GAGCCCGAGG ACGAAGACGG CGAGGACGTG CGCGGGGTGC TCAAGAGGCG CGTGGAGACG
    CGGCAGCACA CCGAGGAGGC TATCCGCCAG CAGGAGGTGG AGCAGCTGGA CTTCCGCGAC
    CTCCTGGGCA AGAAGGTGAG CACCAAGACC TTGTCTGAGG AGGACCTGAA GGAGATCCCT
    GCCGAGCAGA TGGATTTCCG CGCCAATCTG CAGCGGCAAG TCAAGCCGAA GACTGTGTCC
    GAGGAAGAGA GGAAGGTGCA TAGCCCCCAG CAGGTGGACT TCCGCTCGGT CCTGGCCAAG
    AAGGGGACGC CCAAGACCCC CGTACCTGAG AAGCTGCCCC CTCCAAAACC TGCCACCCCA
    GATTTTCGCT CGGTGTTGGG CAGTAAGAAA AAATTACCAG CGGAGAATGG TAGCAGCAAT
    GCTGAGCCTT TGAATGTCAA GGCAGCAGAA GGTCCCAAGC CTGTGGGCAA TGCCCAGCCT
    TCAGGCTTTC TCAAACCTAT GGGCAATGCC AAGCTGGCCG AGACCTCCAA ACACTTGAGT
    AATGCCAAGC CAGCCGAGCT CCCCAAACCT GTGGGCAACG CCAAGCCAGC CGAGATCCCC
    AAACCCGTGG GCAACACCAG GCCAGCTGAG ACCCCCAAAA CCGTGGGCAA TGCCAAGCCA
    ACTGAGACCT TCAAACCCAT GGCCAATACC AAGCCAGCTG AGACCCTAAA GCCTGTGGGC
    AATGCCAAGC CAGCTGAGAC CCTAAAGCCT GTGGGCAATG CCAAGCCAGC TGAGACCCTA
    AAGCCCGTGG GCAATGCCAA GCCAGCTGAG ACCCCCAAGC CCTTGGGTAA TACCAAGCCA
    GCCGAGCCCC CTAAACCAGC TGGGAAAGAA GAGCTTAAGA AGGAGGTTAA GAATGATGTG
    AACTACAAGA GGGGCTCTGC AGGGGCCACA GATAATGAAA AAAGCCCGGA GAGCCAAGGG
    TCAGCCCCAA CCTTCAAGGA AAAGCTACGT GATCTCCGTG TGGCAGAGGG TGAGAAGCTG
    CTGCTCCAGT GCCAGGTATC CTCCGACCCC CCGGCCACCA TCACCTGGAC ACTGAATGGG
    AAGACACTCA AGACCACCAA GTTCATTGTC CTCTCCCAAG AAGGCTCACT GTGCTCCATC
    TCCATCGAGA AGGCCTTGCC CGAGGACAGA GGTTTGTACA AGTGTGTGGC CAAGAACAGC
    GCCGGCCAGG CTGAGTGCTC CTGCCAAGTC ACCGTGGACG ATGCCCCCAC CGGAGAGAAT
    GCCAAGGCCC CGGAGATGAA AGCCCGGAGG CCAAAGAGCT CTCTTCCTCC TGTGCTAGGA
    ACAGAGAGTG ATGCCACTGT GAAAAAGAAA CCTGCCCCCA AGACACCTCC GAAGGCAGCC
    ATGCCCCCTC AGATCATCCA GTTTCCCGAG GACCAAAAGG TGCGAGCGGG AGAGTCTGTG
    GAGCTGTTTG GTAAAGTGGC CGGTACCCAG CCCATCACCT GTACCTGGAT GAAGTTCCGA
    AAGCAGATCC AGGAGAGTGA GCACATCAAG GTGGAGAACA GTGAGAATGG CAGCAAGCTC
    ACCATCCGGG CCGCGCGCCA GGAGCACTGT GGCTGCTACA CTCTGCTGGT GGAGAACAAG
    CTGGGCAGCA GGCAGGCCCA GGTCAACCTC ACTGTTGTGG ACAAGCCGGA CCCCCCCGCA
    GGCACGCCTT GTGCCTCTGA CATCCGCAGC TCCTCGCTGA CCCTGTCCTG GTATGGCTCC
    TCCTACGATG GGGGCAGCGC CGTACAGTCC TACAGCGTCG AGATCTGGGA CTCAGTGGAC
    AAGACGTGGA AGGAACTAGC CACATGCCGC AGCACCTCTT TTAACGTCCA GGACCTGCTG
    TCTGACCGGG AGTATAAATT CCGTGTGCGT GCAATCAACA TCTACGGAAC CAGTGAGCCG
    AGCCAGGAGT CTGAACTCAC AGCGGTGGGA GAGAAACCTG AGGAGCCCAA GGATGAAGTG
    GAGGTGTCAG ATGACGATGA GAAGGAGCCT GAGGTCGATT ACCGGACCGT CACGGTCAAT
    ACGGAACAAA AAGTGTCTGA CTTCTACGAC ATTGAAGAGA GACTAGGATC TGGGAAATTT
    GGACAGGTCT TCCGACTTGT AGAGAAGAAA ACTGGAAAAA TCTGGGCAGG GAAATTCTTC
    AAGGCGTATT CAGCAAAAGA GAAAGAGAAC ATCCGGCAGG AGATCAGCAT CATGAACTGC
    CTCCACCACC CCAAGCTGGT CCAGTGTGTG GACGCCTTCG AAGAGAAGGC CAACATCGTC
    ATGGTCCTGG AGATCGTGTC CGGGGGCGAG CTGTTCGAGC GCATCATTGA CGAGGACTTT
    GAGCTGACGG AGCGCGAGTG CATCAAGTAC ATGCGGCAGA TCTCGGAGGG GGTGGAGTAC
    ATCCACAAGC AGGGCATCGT CCACCTGGAC CTCAAGCCTG AGAACATCAT GTGTGTCAAC
    AAGACGGGCA CCCGGATCAA GCTCATCGAC TTCGGTCTGG CCCGGCGGCT GGAGAATGCG
    GGGTCTCTGA AGGTCCTCTT TGGCACCCCG GAGTTTGTGG CGCCTGAAGT GATCAACTAT
    GAGCCCATTG GCTATGCCAC GGACATGTGG AGCATCGGGG TCATCTGCTA CATCCTGGTC
    AGTGGACTGT CCCCCTTTAT GGGTGACAAC GATAACGAGA CCTTAGCCAA CGTTACCTCA
    GCCACCTGGG ACTTCGACGA TGAGGCCTTC GATGAGATCT CCGACGACGC CAAGGATTTC
    ATCAGCAATT TACTGAAGAA AGATATGAAA AACCGCTTGG ACTGCACCCA GTGCCTCCAG
    CACCCATGGC TAATGAAAGA TACCAAGAAC ATGGAGGCCA AGAAGCTCTC CAAGGACCGG
    ATGAAAAAGT ACATGGCAAG GAGGAAATGG CAGAAAACGG GCAATGCTGT GAGAGCCATT
    GGAAGACTGT CCTCTATGGC AATGATCTCA GGGCTCAGTG GCAGGAAATC CTCAACAGGG
    TCACCAACCA GCCCGCTGAA TGCAGAAAAA TTAGAATCTG AAGAAGATGT CTCCCAAGCT
    TTCCTTGAGG CCGTCGCTGA GGAAAAGCCC CATGTAAAAC CCTATTTTTC TAAGACCATT
    CGTGATTTAG AAGTTGTGGA GGGAAGCGCT GCTAGATTTG ACTGCAAGAT TGAAGGATAC
    CCAGACCCTG AGGTTGTCTG GTTCAAAGAT GACCAGTCAA TCAGGGAGTC CCGCCACTTC
    CAGATAGACT ACGATGAGGA TGGGAACTGC TCTTTAATCA TTAGCGATGT CTGCGGTGAT
    GATGATGCCA AGTACACCTG CAAGGCTGTC AACAGTCTTG GAGAAGCCAC CTGCACAGCA
    GAGCTCATTG TGGAAACCAT GGAGGAAGGA GAAGGGGAAG GGGAAGAAGA AGAGGAGGAA
    GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe236095
    Clone ID Related Accession (Same CDS sequence) XM_021070232.1 , XM_021070231.1 , XM_021070234.1 , XM_021070235.1 , XM_021070233.1
    Accession Version XM_021070231.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5946bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product myosin light chain kinase, smooth muscle isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010455.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGATCACT CGAGAGTCGG CTCCACGCCC CTGACCGAGG CCCCTGCTTT CATTTTGCCC 
    CCTCGGAACC TTTGCATCAA GGAAGGAGCC ACTGCCAAGT TTGAAGGGAG GGTCCGGGGT
    TACCCAGAGC CCCAGGTGAC ATGGCACAGA AACGGACAGC CCATCACCAG TGGAGGTCGC
    TTCCTGCTGG ACTGTGGCAT CCGGGGGACC TTCAGTCTTG TGATTCATTC TGTCTGTGAG
    GAGGACAAGG GAAAGTATAC CTGTGAAGCC ACCAATGGTA GTGGCGCCCG CCAGGTGACA
    GTGGAGTTGA CGGTAGAAGG GGGCTTTGTG AAGAAGCATG GCCAGCCTGT TGTTTCCAAA
    GCCTTGGGGG ACAGATTTGC AGCCCCTGCA GTCGAGACCC GTCCTAGCAT CTGGGGAGAG
    TGCCCACCAA AGTTTGCCAC CAAGCTGGGC CGAGCGGTGG TCAAAGAAGG ACAGATGGGG
    CGATTCTCCT GCAAGATCAC TGGCCGGCCC CAACCGCAGG TCACCTGGCT CAAGGGAGAT
    GTTCCCCTGC AGCCCAGTGC CCGTGTGACC ATGTCTGAGA AGAATGGGGT GCAAGTGCTG
    GAAATCCACG AAGTCAACCG AGACGACGTG GGGGTGTACA CGTGCATGGT GGTGAATGGG
    TCGGGGAAGG CCTCCATGTC AGCTGAGCTC TCCATCCAAG GTTTGGACAA TACCAATAGG
    TCGCTTGTGA GAGGAACAAA GGCGGCCAGT TCAGACATCA GGACAGAAGC GACCAATGGA
    CTCTCACAGG GGCCAAAGCT GGATGGCCTG GAGGCTGCAG CGGAGAGTAA GAACTGCTCC
    AGCGCCCAGA GGGGTGGCTC CCCGACCTGG GCTTCAGGGA GCCAGCCTCA GCCTCCGAGG
    GAATCCAAGC TGGAGCCGTC AGGGGGCTCA GACAGAAAAG CCCTGAGGAG CCCCATCCTG
    CAGAAGACTT CCAGTGCCAT CACCCTGCAG CCTGCAAAAG TCCAGTTGGA ACCAAGGGCT
    CCAGTCTCAG GCACCCTCTT GCCTTCCAGA GAAGAGAGAG AGCGGCCAGC TGCTCCACCT
    GCCGCCACCC TGCCCTCCAG ACAGTCTGGC ACAGGAGGCC AAGAAGCTGT GAGCAAGGCT
    GCTACCAAGC AGGTTGCCAT GGAGAGCCGA AGGGCTGCCA CAGTCCCCAG ATTCGAGAGC
    AAGCCCCAAA GCCAGGAGGT CAGTGAAGAT CAGCCAGTCA AGTTCAAGTG TGAAGTTTCA
    GGGGTTCCAA AGCCCGAAGT GACCTGGTTC CTGGACGGTG CCCCTGTGAA GAGGCGAGAA
    GGCACCTTTG AGGTTTATGA AGCCGGAGGA TCCCACTACC TCTGCCTGCC AAGAGCCCGG
    GCCAGGGACA GCGGGAACTA CAGCTGCACA GCCACCAACA TCCGAGGCCA GGTGTCCTGT
    ACCTGGACCC TCCTGGTGAA AAGGCTGGCC GTGACAGAGG CGCCCCCGTC CTTCTCCAGT
    GTCCTGAAGG ACTGTGCTGT GGTTGAGGGC CAGGACTTCG TGCTGCAGTG CTCCGTGCAG
    GGGACCCCGC TGCCCCAGAT CACTTGGCTG CTCAATGGGC AGCCCATCCA GTACGCCCGC
    TCCACCTGCG AGGCCGGCGT TGCCAAGCTC CACATCCAGG ATGCCCTCCC GGAGGACGAC
    GGCACCTATA CCTGTCTGGC CGAGAACACC TTGGGGCGGG TGTCCTGCAG CGCCCGGGTC
    ACTGTCCATG AAAAGAAGAG TGATGGGAAG AGTGAGTACC TCCTGCCCGC TGCCCCCAGC
    AAGCCCATGG CACCCATCTT CCTGCAGGGT CTCTCTGATC TCAGCGTCAT GGATGGGAGC
    CAGGTCACCA TGACAGTCCA GGTGTCAGGG AACCCACCCC CTGAGGTCAT CTGGCTTCAC
    GATGGGAATG AGATCCAGGA GTCGGAGGAC TTCCACTTTG AGCAGAGGGG CACTCGGCAC
    AGCCTCTGTA TCCAGGAAGT GTTCCCAGAA GACACAGGCA CATACACCTG TGAAGCCTGG
    AACAGCGCAG GAGAGGTCCG CACCCAGGCC GTGCTCACGG TGCAAGAGCC TCAGGACGGC
    ACCCAGCCTT GGTTCATCAG CAAGCCCCGC TCTGTGACAG CCTGCCTGGG CCAGAGTGTC
    CTCATCTCCT GTGCCATAGC TGGGGACCCC TTCCCTACTG TGCACTGGCT CCGAGACGGC
    AAAGCCCTCT CCAAAGACAC TGGCCACTTC GAGGTGCTGC AGAATGAGGA CGTGTTCACC
    CTGGTTCTCA AGAACGTGCA GCCCTGGCAC GCCGGCCAGT ATGAGATCCT GCTCAAGAAC
    CGGGTTGGCG AGTGCAGCTG CCAGGTGTCC CTGGTGCTAC AGAGCAGCCC TGCCAGAGCC
    CCCCTGCGGG GGAGGGAGCC TGCCAGCTGT GAGGGCCAAG GAGCTGGTGC TGATGGTGGT
    GGTGCTGGCA GAGACGGCTA CGGGAGCCTG AGGCCCGGCT GGCCAGCAAG AGGGCAGGGT
    GAGCCCGAGG ACGAAGACGG CGAGGACGTG CGCGGGGTGC TCAAGAGGCG CGTGGAGACG
    CGGCAGCACA CCGAGGAGGC TATCCGCCAG CAGGAGGTGG AGCAGCTGGA CTTCCGCGAC
    CTCCTGGGCA AGAAGGTGAG CACCAAGACC TTGTCTGAGG AGGACCTGAA GGAGATCCCT
    GCCGAGCAGA TGGATTTCCG CGCCAATCTG CAGCGGCAAG TCAAGCCGAA GACTGTGTCC
    GAGGAAGAGA GGAAGGTGCA TAGCCCCCAG CAGGTGGACT TCCGCTCGGT CCTGGCCAAG
    AAGGGGACGC CCAAGACCCC CGTACCTGAG AAGCTGCCCC CTCCAAAACC TGCCACCCCA
    GATTTTCGCT CGGTGTTGGG CAGTAAGAAA AAATTACCAG CGGAGAATGG TAGCAGCAAT
    GCTGAGCCTT TGAATGTCAA GGCAGCAGAA GGTCCCAAGC CTGTGGGCAA TGCCCAGCCT
    TCAGGCTTTC TCAAACCTAT GGGCAATGCC AAGCTGGCCG AGACCTCCAA ACACTTGAGT
    AATGCCAAGC CAGCCGAGCT CCCCAAACCT GTGGGCAACG CCAAGCCAGC CGAGATCCCC
    AAACCCGTGG GCAACACCAG GCCAGCTGAG ACCCCCAAAA CCGTGGGCAA TGCCAAGCCA
    ACTGAGACCT TCAAACCCAT GGCCAATACC AAGCCAGCTG AGACCCTAAA GCCTGTGGGC
    AATGCCAAGC CAGCTGAGAC CCTAAAGCCT GTGGGCAATG CCAAGCCAGC TGAGACCCTA
    AAGCCCGTGG GCAATGCCAA GCCAGCTGAG ACCCCCAAGC CCTTGGGTAA TACCAAGCCA
    GCCGAGCCCC CTAAACCAGC TGGGAAAGAA GAGCTTAAGA AGGAGGTTAA GAATGATGTG
    AACTACAAGA GGGGCTCTGC AGGGGCCACA GATAATGAAA AAAGCCCGGA GAGCCAAGGG
    TCAGCCCCAA CCTTCAAGGA AAAGCTACGT GATCTCCGTG TGGCAGAGGG TGAGAAGCTG
    CTGCTCCAGT GCCAGGTATC CTCCGACCCC CCGGCCACCA TCACCTGGAC ACTGAATGGG
    AAGACACTCA AGACCACCAA GTTCATTGTC CTCTCCCAAG AAGGCTCACT GTGCTCCATC
    TCCATCGAGA AGGCCTTGCC CGAGGACAGA GGTTTGTACA AGTGTGTGGC CAAGAACAGC
    GCCGGCCAGG CTGAGTGCTC CTGCCAAGTC ACCGTGGACG ATGCCCCCAC CGGAGAGAAT
    GCCAAGGCCC CGGAGATGAA AGCCCGGAGG CCAAAGAGCT CTCTTCCTCC TGTGCTAGGA
    ACAGAGAGTG ATGCCACTGT GAAAAAGAAA CCTGCCCCCA AGACACCTCC GAAGGCAGCC
    ATGCCCCCTC AGATCATCCA GTTTCCCGAG GACCAAAAGG TGCGAGCGGG AGAGTCTGTG
    GAGCTGTTTG GTAAAGTGGC CGGTACCCAG CCCATCACCT GTACCTGGAT GAAGTTCCGA
    AAGCAGATCC AGGAGAGTGA GCACATCAAG GTGGAGAACA GTGAGAATGG CAGCAAGCTC
    ACCATCCGGG CCGCGCGCCA GGAGCACTGT GGCTGCTACA CTCTGCTGGT GGAGAACAAG
    CTGGGCAGCA GGCAGGCCCA GGTCAACCTC ACTGTTGTGG ACAAGCCGGA CCCCCCCGCA
    GGCACGCCTT GTGCCTCTGA CATCCGCAGC TCCTCGCTGA CCCTGTCCTG GTATGGCTCC
    TCCTACGATG GGGGCAGCGC CGTACAGTCC TACAGCGTCG AGATCTGGGA CTCAGTGGAC
    AAGACGTGGA AGGAACTAGC CACATGCCGC AGCACCTCTT TTAACGTCCA GGACCTGCTG
    TCTGACCGGG AGTATAAATT CCGTGTGCGT GCAATCAACA TCTACGGAAC CAGTGAGCCG
    AGCCAGGAGT CTGAACTCAC AGCGGTGGGA GAGAAACCTG AGGAGCCCAA GGATGAAGTG
    GAGGTGTCAG ATGACGATGA GAAGGAGCCT GAGGTCGATT ACCGGACCGT CACGGTCAAT
    ACGGAACAAA AAGTGTCTGA CTTCTACGAC ATTGAAGAGA GACTAGGATC TGGGAAATTT
    GGACAGGTCT TCCGACTTGT AGAGAAGAAA ACTGGAAAAA TCTGGGCAGG GAAATTCTTC
    AAGGCGTATT CAGCAAAAGA GAAAGAGAAC ATCCGGCAGG AGATCAGCAT CATGAACTGC
    CTCCACCACC CCAAGCTGGT CCAGTGTGTG GACGCCTTCG AAGAGAAGGC CAACATCGTC
    ATGGTCCTGG AGATCGTGTC CGGGGGCGAG CTGTTCGAGC GCATCATTGA CGAGGACTTT
    GAGCTGACGG AGCGCGAGTG CATCAAGTAC ATGCGGCAGA TCTCGGAGGG GGTGGAGTAC
    ATCCACAAGC AGGGCATCGT CCACCTGGAC CTCAAGCCTG AGAACATCAT GTGTGTCAAC
    AAGACGGGCA CCCGGATCAA GCTCATCGAC TTCGGTCTGG CCCGGCGGCT GGAGAATGCG
    GGGTCTCTGA AGGTCCTCTT TGGCACCCCG GAGTTTGTGG CGCCTGAAGT GATCAACTAT
    GAGCCCATTG GCTATGCCAC GGACATGTGG AGCATCGGGG TCATCTGCTA CATCCTGGTC
    AGTGGACTGT CCCCCTTTAT GGGTGACAAC GATAACGAGA CCTTAGCCAA CGTTACCTCA
    GCCACCTGGG ACTTCGACGA TGAGGCCTTC GATGAGATCT CCGACGACGC CAAGGATTTC
    ATCAGCAATT TACTGAAGAA AGATATGAAA AACCGCTTGG ACTGCACCCA GTGCCTCCAG
    CACCCATGGC TAATGAAAGA TACCAAGAAC ATGGAGGCCA AGAAGCTCTC CAAGGACCGG
    ATGAAAAAGT ACATGGCAAG GAGGAAATGG CAGAAAACGG GCAATGCTGT GAGAGCCATT
    GGAAGACTGT CCTCTATGGC AATGATCTCA GGGCTCAGTG GCAGGAAATC CTCAACAGGG
    TCACCAACCA GCCCGCTGAA TGCAGAAAAA TTAGAATCTG AAGAAGATGT CTCCCAAGCT
    TTCCTTGAGG CCGTCGCTGA GGAAAAGCCC CATGTAAAAC CCTATTTTTC TAAGACCATT
    CGTGATTTAG AAGTTGTGGA GGGAAGCGCT GCTAGATTTG ACTGCAAGAT TGAAGGATAC
    CCAGACCCTG AGGTTGTCTG GTTCAAAGAT GACCAGTCAA TCAGGGAGTC CCGCCACTTC
    CAGATAGACT ACGATGAGGA TGGGAACTGC TCTTTAATCA TTAGCGATGT CTGCGGTGAT
    GATGATGCCA AGTACACCTG CAAGGCTGTC AACAGTCTTG GAGAAGCCAC CTGCACAGCA
    GAGCTCATTG TGGAAACCAT GGAGGAAGGA GAAGGGGAAG GGGAAGAAGA AGAGGAGGAA
    GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020925890.1
    CDS415..6360
    Translation

    Target ORF information:

    RefSeq Version XM_021070231.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa myosin light chain kinase (MYLK), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021070231.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGATCACT CGAGAGTCGG CTCCACGCCC CTGACCGAGG CCCCTGCTTT CATTTTGCCC 
    CCTCGGAACC TTTGCATCAA GGAAGGAGCC ACTGCCAAGT TTGAAGGGAG GGTCCGGGGT
    TACCCAGAGC CCCAGGTGAC ATGGCACAGA AACGGACAGC CCATCACCAG TGGAGGTCGC
    TTCCTGCTGG ACTGTGGCAT CCGGGGGACC TTCAGTCTTG TGATTCATTC TGTCTGTGAG
    GAGGACAAGG GAAAGTATAC CTGTGAAGCC ACCAATGGTA GTGGCGCCCG CCAGGTGACA
    GTGGAGTTGA CGGTAGAAGG GGGCTTTGTG AAGAAGCATG GCCAGCCTGT TGTTTCCAAA
    GCCTTGGGGG ACAGATTTGC AGCCCCTGCA GTCGAGACCC GTCCTAGCAT CTGGGGAGAG
    TGCCCACCAA AGTTTGCCAC CAAGCTGGGC CGAGCGGTGG TCAAAGAAGG ACAGATGGGG
    CGATTCTCCT GCAAGATCAC TGGCCGGCCC CAACCGCAGG TCACCTGGCT CAAGGGAGAT
    GTTCCCCTGC AGCCCAGTGC CCGTGTGACC ATGTCTGAGA AGAATGGGGT GCAAGTGCTG
    GAAATCCACG AAGTCAACCG AGACGACGTG GGGGTGTACA CGTGCATGGT GGTGAATGGG
    TCGGGGAAGG CCTCCATGTC AGCTGAGCTC TCCATCCAAG GTTTGGACAA TACCAATAGG
    TCGCTTGTGA GAGGAACAAA GGCGGCCAGT TCAGACATCA GGACAGAAGC GACCAATGGA
    CTCTCACAGG GGCCAAAGCT GGATGGCCTG GAGGCTGCAG CGGAGAGTAA GAACTGCTCC
    AGCGCCCAGA GGGGTGGCTC CCCGACCTGG GCTTCAGGGA GCCAGCCTCA GCCTCCGAGG
    GAATCCAAGC TGGAGCCGTC AGGGGGCTCA GACAGAAAAG CCCTGAGGAG CCCCATCCTG
    CAGAAGACTT CCAGTGCCAT CACCCTGCAG CCTGCAAAAG TCCAGTTGGA ACCAAGGGCT
    CCAGTCTCAG GCACCCTCTT GCCTTCCAGA GAAGAGAGAG AGCGGCCAGC TGCTCCACCT
    GCCGCCACCC TGCCCTCCAG ACAGTCTGGC ACAGGAGGCC AAGAAGCTGT GAGCAAGGCT
    GCTACCAAGC AGGTTGCCAT GGAGAGCCGA AGGGCTGCCA CAGTCCCCAG ATTCGAGAGC
    AAGCCCCAAA GCCAGGAGGT CAGTGAAGAT CAGCCAGTCA AGTTCAAGTG TGAAGTTTCA
    GGGGTTCCAA AGCCCGAAGT GACCTGGTTC CTGGACGGTG CCCCTGTGAA GAGGCGAGAA
    GGCACCTTTG AGGTTTATGA AGCCGGAGGA TCCCACTACC TCTGCCTGCC AAGAGCCCGG
    GCCAGGGACA GCGGGAACTA CAGCTGCACA GCCACCAACA TCCGAGGCCA GGTGTCCTGT
    ACCTGGACCC TCCTGGTGAA AAGGCTGGCC GTGACAGAGG CGCCCCCGTC CTTCTCCAGT
    GTCCTGAAGG ACTGTGCTGT GGTTGAGGGC CAGGACTTCG TGCTGCAGTG CTCCGTGCAG
    GGGACCCCGC TGCCCCAGAT CACTTGGCTG CTCAATGGGC AGCCCATCCA GTACGCCCGC
    TCCACCTGCG AGGCCGGCGT TGCCAAGCTC CACATCCAGG ATGCCCTCCC GGAGGACGAC
    GGCACCTATA CCTGTCTGGC CGAGAACACC TTGGGGCGGG TGTCCTGCAG CGCCCGGGTC
    ACTGTCCATG AAAAGAAGAG TGATGGGAAG AGTGAGTACC TCCTGCCCGC TGCCCCCAGC
    AAGCCCATGG CACCCATCTT CCTGCAGGGT CTCTCTGATC TCAGCGTCAT GGATGGGAGC
    CAGGTCACCA TGACAGTCCA GGTGTCAGGG AACCCACCCC CTGAGGTCAT CTGGCTTCAC
    GATGGGAATG AGATCCAGGA GTCGGAGGAC TTCCACTTTG AGCAGAGGGG CACTCGGCAC
    AGCCTCTGTA TCCAGGAAGT GTTCCCAGAA GACACAGGCA CATACACCTG TGAAGCCTGG
    AACAGCGCAG GAGAGGTCCG CACCCAGGCC GTGCTCACGG TGCAAGAGCC TCAGGACGGC
    ACCCAGCCTT GGTTCATCAG CAAGCCCCGC TCTGTGACAG CCTGCCTGGG CCAGAGTGTC
    CTCATCTCCT GTGCCATAGC TGGGGACCCC TTCCCTACTG TGCACTGGCT CCGAGACGGC
    AAAGCCCTCT CCAAAGACAC TGGCCACTTC GAGGTGCTGC AGAATGAGGA CGTGTTCACC
    CTGGTTCTCA AGAACGTGCA GCCCTGGCAC GCCGGCCAGT ATGAGATCCT GCTCAAGAAC
    CGGGTTGGCG AGTGCAGCTG CCAGGTGTCC CTGGTGCTAC AGAGCAGCCC TGCCAGAGCC
    CCCCTGCGGG GGAGGGAGCC TGCCAGCTGT GAGGGCCAAG GAGCTGGTGC TGATGGTGGT
    GGTGCTGGCA GAGACGGCTA CGGGAGCCTG AGGCCCGGCT GGCCAGCAAG AGGGCAGGGT
    GAGCCCGAGG ACGAAGACGG CGAGGACGTG CGCGGGGTGC TCAAGAGGCG CGTGGAGACG
    CGGCAGCACA CCGAGGAGGC TATCCGCCAG CAGGAGGTGG AGCAGCTGGA CTTCCGCGAC
    CTCCTGGGCA AGAAGGTGAG CACCAAGACC TTGTCTGAGG AGGACCTGAA GGAGATCCCT
    GCCGAGCAGA TGGATTTCCG CGCCAATCTG CAGCGGCAAG TCAAGCCGAA GACTGTGTCC
    GAGGAAGAGA GGAAGGTGCA TAGCCCCCAG CAGGTGGACT TCCGCTCGGT CCTGGCCAAG
    AAGGGGACGC CCAAGACCCC CGTACCTGAG AAGCTGCCCC CTCCAAAACC TGCCACCCCA
    GATTTTCGCT CGGTGTTGGG CAGTAAGAAA AAATTACCAG CGGAGAATGG TAGCAGCAAT
    GCTGAGCCTT TGAATGTCAA GGCAGCAGAA GGTCCCAAGC CTGTGGGCAA TGCCCAGCCT
    TCAGGCTTTC TCAAACCTAT GGGCAATGCC AAGCTGGCCG AGACCTCCAA ACACTTGAGT
    AATGCCAAGC CAGCCGAGCT CCCCAAACCT GTGGGCAACG CCAAGCCAGC CGAGATCCCC
    AAACCCGTGG GCAACACCAG GCCAGCTGAG ACCCCCAAAA CCGTGGGCAA TGCCAAGCCA
    ACTGAGACCT TCAAACCCAT GGCCAATACC AAGCCAGCTG AGACCCTAAA GCCTGTGGGC
    AATGCCAAGC CAGCTGAGAC CCTAAAGCCT GTGGGCAATG CCAAGCCAGC TGAGACCCTA
    AAGCCCGTGG GCAATGCCAA GCCAGCTGAG ACCCCCAAGC CCTTGGGTAA TACCAAGCCA
    GCCGAGCCCC CTAAACCAGC TGGGAAAGAA GAGCTTAAGA AGGAGGTTAA GAATGATGTG
    AACTACAAGA GGGGCTCTGC AGGGGCCACA GATAATGAAA AAAGCCCGGA GAGCCAAGGG
    TCAGCCCCAA CCTTCAAGGA AAAGCTACGT GATCTCCGTG TGGCAGAGGG TGAGAAGCTG
    CTGCTCCAGT GCCAGGTATC CTCCGACCCC CCGGCCACCA TCACCTGGAC ACTGAATGGG
    AAGACACTCA AGACCACCAA GTTCATTGTC CTCTCCCAAG AAGGCTCACT GTGCTCCATC
    TCCATCGAGA AGGCCTTGCC CGAGGACAGA GGTTTGTACA AGTGTGTGGC CAAGAACAGC
    GCCGGCCAGG CTGAGTGCTC CTGCCAAGTC ACCGTGGACG ATGCCCCCAC CGGAGAGAAT
    GCCAAGGCCC CGGAGATGAA AGCCCGGAGG CCAAAGAGCT CTCTTCCTCC TGTGCTAGGA
    ACAGAGAGTG ATGCCACTGT GAAAAAGAAA CCTGCCCCCA AGACACCTCC GAAGGCAGCC
    ATGCCCCCTC AGATCATCCA GTTTCCCGAG GACCAAAAGG TGCGAGCGGG AGAGTCTGTG
    GAGCTGTTTG GTAAAGTGGC CGGTACCCAG CCCATCACCT GTACCTGGAT GAAGTTCCGA
    AAGCAGATCC AGGAGAGTGA GCACATCAAG GTGGAGAACA GTGAGAATGG CAGCAAGCTC
    ACCATCCGGG CCGCGCGCCA GGAGCACTGT GGCTGCTACA CTCTGCTGGT GGAGAACAAG
    CTGGGCAGCA GGCAGGCCCA GGTCAACCTC ACTGTTGTGG ACAAGCCGGA CCCCCCCGCA
    GGCACGCCTT GTGCCTCTGA CATCCGCAGC TCCTCGCTGA CCCTGTCCTG GTATGGCTCC
    TCCTACGATG GGGGCAGCGC CGTACAGTCC TACAGCGTCG AGATCTGGGA CTCAGTGGAC
    AAGACGTGGA AGGAACTAGC CACATGCCGC AGCACCTCTT TTAACGTCCA GGACCTGCTG
    TCTGACCGGG AGTATAAATT CCGTGTGCGT GCAATCAACA TCTACGGAAC CAGTGAGCCG
    AGCCAGGAGT CTGAACTCAC AGCGGTGGGA GAGAAACCTG AGGAGCCCAA GGATGAAGTG
    GAGGTGTCAG ATGACGATGA GAAGGAGCCT GAGGTCGATT ACCGGACCGT CACGGTCAAT
    ACGGAACAAA AAGTGTCTGA CTTCTACGAC ATTGAAGAGA GACTAGGATC TGGGAAATTT
    GGACAGGTCT TCCGACTTGT AGAGAAGAAA ACTGGAAAAA TCTGGGCAGG GAAATTCTTC
    AAGGCGTATT CAGCAAAAGA GAAAGAGAAC ATCCGGCAGG AGATCAGCAT CATGAACTGC
    CTCCACCACC CCAAGCTGGT CCAGTGTGTG GACGCCTTCG AAGAGAAGGC CAACATCGTC
    ATGGTCCTGG AGATCGTGTC CGGGGGCGAG CTGTTCGAGC GCATCATTGA CGAGGACTTT
    GAGCTGACGG AGCGCGAGTG CATCAAGTAC ATGCGGCAGA TCTCGGAGGG GGTGGAGTAC
    ATCCACAAGC AGGGCATCGT CCACCTGGAC CTCAAGCCTG AGAACATCAT GTGTGTCAAC
    AAGACGGGCA CCCGGATCAA GCTCATCGAC TTCGGTCTGG CCCGGCGGCT GGAGAATGCG
    GGGTCTCTGA AGGTCCTCTT TGGCACCCCG GAGTTTGTGG CGCCTGAAGT GATCAACTAT
    GAGCCCATTG GCTATGCCAC GGACATGTGG AGCATCGGGG TCATCTGCTA CATCCTGGTC
    AGTGGACTGT CCCCCTTTAT GGGTGACAAC GATAACGAGA CCTTAGCCAA CGTTACCTCA
    GCCACCTGGG ACTTCGACGA TGAGGCCTTC GATGAGATCT CCGACGACGC CAAGGATTTC
    ATCAGCAATT TACTGAAGAA AGATATGAAA AACCGCTTGG ACTGCACCCA GTGCCTCCAG
    CACCCATGGC TAATGAAAGA TACCAAGAAC ATGGAGGCCA AGAAGCTCTC CAAGGACCGG
    ATGAAAAAGT ACATGGCAAG GAGGAAATGG CAGAAAACGG GCAATGCTGT GAGAGCCATT
    GGAAGACTGT CCTCTATGGC AATGATCTCA GGGCTCAGTG GCAGGAAATC CTCAACAGGG
    TCACCAACCA GCCCGCTGAA TGCAGAAAAA TTAGAATCTG AAGAAGATGT CTCCCAAGCT
    TTCCTTGAGG CCGTCGCTGA GGAAAAGCCC CATGTAAAAC CCTATTTTTC TAAGACCATT
    CGTGATTTAG AAGTTGTGGA GGGAAGCGCT GCTAGATTTG ACTGCAAGAT TGAAGGATAC
    CCAGACCCTG AGGTTGTCTG GTTCAAAGAT GACCAGTCAA TCAGGGAGTC CCGCCACTTC
    CAGATAGACT ACGATGAGGA TGGGAACTGC TCTTTAATCA TTAGCGATGT CTGCGGTGAT
    GATGATGCCA AGTACACCTG CAAGGCTGTC AACAGTCTTG GAGAAGCCAC CTGCACAGCA
    GAGCTCATTG TGGAAACCAT GGAGGAAGGA GAAGGGGAAG GGGAAGAAGA AGAGGAGGAA
    GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe236095
    Clone ID Related Accession (Same CDS sequence) XM_021070232.1 , XM_021070231.1 , XM_021070234.1 , XM_021070235.1 , XM_021070233.1
    Accession Version XM_021070234.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5946bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product myosin light chain kinase, smooth muscle isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010455.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGATCACT CGAGAGTCGG CTCCACGCCC CTGACCGAGG CCCCTGCTTT CATTTTGCCC 
    CCTCGGAACC TTTGCATCAA GGAAGGAGCC ACTGCCAAGT TTGAAGGGAG GGTCCGGGGT
    TACCCAGAGC CCCAGGTGAC ATGGCACAGA AACGGACAGC CCATCACCAG TGGAGGTCGC
    TTCCTGCTGG ACTGTGGCAT CCGGGGGACC TTCAGTCTTG TGATTCATTC TGTCTGTGAG
    GAGGACAAGG GAAAGTATAC CTGTGAAGCC ACCAATGGTA GTGGCGCCCG CCAGGTGACA
    GTGGAGTTGA CGGTAGAAGG GGGCTTTGTG AAGAAGCATG GCCAGCCTGT TGTTTCCAAA
    GCCTTGGGGG ACAGATTTGC AGCCCCTGCA GTCGAGACCC GTCCTAGCAT CTGGGGAGAG
    TGCCCACCAA AGTTTGCCAC CAAGCTGGGC CGAGCGGTGG TCAAAGAAGG ACAGATGGGG
    CGATTCTCCT GCAAGATCAC TGGCCGGCCC CAACCGCAGG TCACCTGGCT CAAGGGAGAT
    GTTCCCCTGC AGCCCAGTGC CCGTGTGACC ATGTCTGAGA AGAATGGGGT GCAAGTGCTG
    GAAATCCACG AAGTCAACCG AGACGACGTG GGGGTGTACA CGTGCATGGT GGTGAATGGG
    TCGGGGAAGG CCTCCATGTC AGCTGAGCTC TCCATCCAAG GTTTGGACAA TACCAATAGG
    TCGCTTGTGA GAGGAACAAA GGCGGCCAGT TCAGACATCA GGACAGAAGC GACCAATGGA
    CTCTCACAGG GGCCAAAGCT GGATGGCCTG GAGGCTGCAG CGGAGAGTAA GAACTGCTCC
    AGCGCCCAGA GGGGTGGCTC CCCGACCTGG GCTTCAGGGA GCCAGCCTCA GCCTCCGAGG
    GAATCCAAGC TGGAGCCGTC AGGGGGCTCA GACAGAAAAG CCCTGAGGAG CCCCATCCTG
    CAGAAGACTT CCAGTGCCAT CACCCTGCAG CCTGCAAAAG TCCAGTTGGA ACCAAGGGCT
    CCAGTCTCAG GCACCCTCTT GCCTTCCAGA GAAGAGAGAG AGCGGCCAGC TGCTCCACCT
    GCCGCCACCC TGCCCTCCAG ACAGTCTGGC ACAGGAGGCC AAGAAGCTGT GAGCAAGGCT
    GCTACCAAGC AGGTTGCCAT GGAGAGCCGA AGGGCTGCCA CAGTCCCCAG ATTCGAGAGC
    AAGCCCCAAA GCCAGGAGGT CAGTGAAGAT CAGCCAGTCA AGTTCAAGTG TGAAGTTTCA
    GGGGTTCCAA AGCCCGAAGT GACCTGGTTC CTGGACGGTG CCCCTGTGAA GAGGCGAGAA
    GGCACCTTTG AGGTTTATGA AGCCGGAGGA TCCCACTACC TCTGCCTGCC AAGAGCCCGG
    GCCAGGGACA GCGGGAACTA CAGCTGCACA GCCACCAACA TCCGAGGCCA GGTGTCCTGT
    ACCTGGACCC TCCTGGTGAA AAGGCTGGCC GTGACAGAGG CGCCCCCGTC CTTCTCCAGT
    GTCCTGAAGG ACTGTGCTGT GGTTGAGGGC CAGGACTTCG TGCTGCAGTG CTCCGTGCAG
    GGGACCCCGC TGCCCCAGAT CACTTGGCTG CTCAATGGGC AGCCCATCCA GTACGCCCGC
    TCCACCTGCG AGGCCGGCGT TGCCAAGCTC CACATCCAGG ATGCCCTCCC GGAGGACGAC
    GGCACCTATA CCTGTCTGGC CGAGAACACC TTGGGGCGGG TGTCCTGCAG CGCCCGGGTC
    ACTGTCCATG AAAAGAAGAG TGATGGGAAG AGTGAGTACC TCCTGCCCGC TGCCCCCAGC
    AAGCCCATGG CACCCATCTT CCTGCAGGGT CTCTCTGATC TCAGCGTCAT GGATGGGAGC
    CAGGTCACCA TGACAGTCCA GGTGTCAGGG AACCCACCCC CTGAGGTCAT CTGGCTTCAC
    GATGGGAATG AGATCCAGGA GTCGGAGGAC TTCCACTTTG AGCAGAGGGG CACTCGGCAC
    AGCCTCTGTA TCCAGGAAGT GTTCCCAGAA GACACAGGCA CATACACCTG TGAAGCCTGG
    AACAGCGCAG GAGAGGTCCG CACCCAGGCC GTGCTCACGG TGCAAGAGCC TCAGGACGGC
    ACCCAGCCTT GGTTCATCAG CAAGCCCCGC TCTGTGACAG CCTGCCTGGG CCAGAGTGTC
    CTCATCTCCT GTGCCATAGC TGGGGACCCC TTCCCTACTG TGCACTGGCT CCGAGACGGC
    AAAGCCCTCT CCAAAGACAC TGGCCACTTC GAGGTGCTGC AGAATGAGGA CGTGTTCACC
    CTGGTTCTCA AGAACGTGCA GCCCTGGCAC GCCGGCCAGT ATGAGATCCT GCTCAAGAAC
    CGGGTTGGCG AGTGCAGCTG CCAGGTGTCC CTGGTGCTAC AGAGCAGCCC TGCCAGAGCC
    CCCCTGCGGG GGAGGGAGCC TGCCAGCTGT GAGGGCCAAG GAGCTGGTGC TGATGGTGGT
    GGTGCTGGCA GAGACGGCTA CGGGAGCCTG AGGCCCGGCT GGCCAGCAAG AGGGCAGGGT
    GAGCCCGAGG ACGAAGACGG CGAGGACGTG CGCGGGGTGC TCAAGAGGCG CGTGGAGACG
    CGGCAGCACA CCGAGGAGGC TATCCGCCAG CAGGAGGTGG AGCAGCTGGA CTTCCGCGAC
    CTCCTGGGCA AGAAGGTGAG CACCAAGACC TTGTCTGAGG AGGACCTGAA GGAGATCCCT
    GCCGAGCAGA TGGATTTCCG CGCCAATCTG CAGCGGCAAG TCAAGCCGAA GACTGTGTCC
    GAGGAAGAGA GGAAGGTGCA TAGCCCCCAG CAGGTGGACT TCCGCTCGGT CCTGGCCAAG
    AAGGGGACGC CCAAGACCCC CGTACCTGAG AAGCTGCCCC CTCCAAAACC TGCCACCCCA
    GATTTTCGCT CGGTGTTGGG CAGTAAGAAA AAATTACCAG CGGAGAATGG TAGCAGCAAT
    GCTGAGCCTT TGAATGTCAA GGCAGCAGAA GGTCCCAAGC CTGTGGGCAA TGCCCAGCCT
    TCAGGCTTTC TCAAACCTAT GGGCAATGCC AAGCTGGCCG AGACCTCCAA ACACTTGAGT
    AATGCCAAGC CAGCCGAGCT CCCCAAACCT GTGGGCAACG CCAAGCCAGC CGAGATCCCC
    AAACCCGTGG GCAACACCAG GCCAGCTGAG ACCCCCAAAA CCGTGGGCAA TGCCAAGCCA
    ACTGAGACCT TCAAACCCAT GGCCAATACC AAGCCAGCTG AGACCCTAAA GCCTGTGGGC
    AATGCCAAGC CAGCTGAGAC CCTAAAGCCT GTGGGCAATG CCAAGCCAGC TGAGACCCTA
    AAGCCCGTGG GCAATGCCAA GCCAGCTGAG ACCCCCAAGC CCTTGGGTAA TACCAAGCCA
    GCCGAGCCCC CTAAACCAGC TGGGAAAGAA GAGCTTAAGA AGGAGGTTAA GAATGATGTG
    AACTACAAGA GGGGCTCTGC AGGGGCCACA GATAATGAAA AAAGCCCGGA GAGCCAAGGG
    TCAGCCCCAA CCTTCAAGGA AAAGCTACGT GATCTCCGTG TGGCAGAGGG TGAGAAGCTG
    CTGCTCCAGT GCCAGGTATC CTCCGACCCC CCGGCCACCA TCACCTGGAC ACTGAATGGG
    AAGACACTCA AGACCACCAA GTTCATTGTC CTCTCCCAAG AAGGCTCACT GTGCTCCATC
    TCCATCGAGA AGGCCTTGCC CGAGGACAGA GGTTTGTACA AGTGTGTGGC CAAGAACAGC
    GCCGGCCAGG CTGAGTGCTC CTGCCAAGTC ACCGTGGACG ATGCCCCCAC CGGAGAGAAT
    GCCAAGGCCC CGGAGATGAA AGCCCGGAGG CCAAAGAGCT CTCTTCCTCC TGTGCTAGGA
    ACAGAGAGTG ATGCCACTGT GAAAAAGAAA CCTGCCCCCA AGACACCTCC GAAGGCAGCC
    ATGCCCCCTC AGATCATCCA GTTTCCCGAG GACCAAAAGG TGCGAGCGGG AGAGTCTGTG
    GAGCTGTTTG GTAAAGTGGC CGGTACCCAG CCCATCACCT GTACCTGGAT GAAGTTCCGA
    AAGCAGATCC AGGAGAGTGA GCACATCAAG GTGGAGAACA GTGAGAATGG CAGCAAGCTC
    ACCATCCGGG CCGCGCGCCA GGAGCACTGT GGCTGCTACA CTCTGCTGGT GGAGAACAAG
    CTGGGCAGCA GGCAGGCCCA GGTCAACCTC ACTGTTGTGG ACAAGCCGGA CCCCCCCGCA
    GGCACGCCTT GTGCCTCTGA CATCCGCAGC TCCTCGCTGA CCCTGTCCTG GTATGGCTCC
    TCCTACGATG GGGGCAGCGC CGTACAGTCC TACAGCGTCG AGATCTGGGA CTCAGTGGAC
    AAGACGTGGA AGGAACTAGC CACATGCCGC AGCACCTCTT TTAACGTCCA GGACCTGCTG
    TCTGACCGGG AGTATAAATT CCGTGTGCGT GCAATCAACA TCTACGGAAC CAGTGAGCCG
    AGCCAGGAGT CTGAACTCAC AGCGGTGGGA GAGAAACCTG AGGAGCCCAA GGATGAAGTG
    GAGGTGTCAG ATGACGATGA GAAGGAGCCT GAGGTCGATT ACCGGACCGT CACGGTCAAT
    ACGGAACAAA AAGTGTCTGA CTTCTACGAC ATTGAAGAGA GACTAGGATC TGGGAAATTT
    GGACAGGTCT TCCGACTTGT AGAGAAGAAA ACTGGAAAAA TCTGGGCAGG GAAATTCTTC
    AAGGCGTATT CAGCAAAAGA GAAAGAGAAC ATCCGGCAGG AGATCAGCAT CATGAACTGC
    CTCCACCACC CCAAGCTGGT CCAGTGTGTG GACGCCTTCG AAGAGAAGGC CAACATCGTC
    ATGGTCCTGG AGATCGTGTC CGGGGGCGAG CTGTTCGAGC GCATCATTGA CGAGGACTTT
    GAGCTGACGG AGCGCGAGTG CATCAAGTAC ATGCGGCAGA TCTCGGAGGG GGTGGAGTAC
    ATCCACAAGC AGGGCATCGT CCACCTGGAC CTCAAGCCTG AGAACATCAT GTGTGTCAAC
    AAGACGGGCA CCCGGATCAA GCTCATCGAC TTCGGTCTGG CCCGGCGGCT GGAGAATGCG
    GGGTCTCTGA AGGTCCTCTT TGGCACCCCG GAGTTTGTGG CGCCTGAAGT GATCAACTAT
    GAGCCCATTG GCTATGCCAC GGACATGTGG AGCATCGGGG TCATCTGCTA CATCCTGGTC
    AGTGGACTGT CCCCCTTTAT GGGTGACAAC GATAACGAGA CCTTAGCCAA CGTTACCTCA
    GCCACCTGGG ACTTCGACGA TGAGGCCTTC GATGAGATCT CCGACGACGC CAAGGATTTC
    ATCAGCAATT TACTGAAGAA AGATATGAAA AACCGCTTGG ACTGCACCCA GTGCCTCCAG
    CACCCATGGC TAATGAAAGA TACCAAGAAC ATGGAGGCCA AGAAGCTCTC CAAGGACCGG
    ATGAAAAAGT ACATGGCAAG GAGGAAATGG CAGAAAACGG GCAATGCTGT GAGAGCCATT
    GGAAGACTGT CCTCTATGGC AATGATCTCA GGGCTCAGTG GCAGGAAATC CTCAACAGGG
    TCACCAACCA GCCCGCTGAA TGCAGAAAAA TTAGAATCTG AAGAAGATGT CTCCCAAGCT
    TTCCTTGAGG CCGTCGCTGA GGAAAAGCCC CATGTAAAAC CCTATTTTTC TAAGACCATT
    CGTGATTTAG AAGTTGTGGA GGGAAGCGCT GCTAGATTTG ACTGCAAGAT TGAAGGATAC
    CCAGACCCTG AGGTTGTCTG GTTCAAAGAT GACCAGTCAA TCAGGGAGTC CCGCCACTTC
    CAGATAGACT ACGATGAGGA TGGGAACTGC TCTTTAATCA TTAGCGATGT CTGCGGTGAT
    GATGATGCCA AGTACACCTG CAAGGCTGTC AACAGTCTTG GAGAAGCCAC CTGCACAGCA
    GAGCTCATTG TGGAAACCAT GGAGGAAGGA GAAGGGGAAG GGGAAGAAGA AGAGGAGGAA
    GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020925893.1
    CDS530..6475
    Translation

    Target ORF information:

    RefSeq Version XM_021070234.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa myosin light chain kinase (MYLK), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021070234.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGATCACT CGAGAGTCGG CTCCACGCCC CTGACCGAGG CCCCTGCTTT CATTTTGCCC 
    CCTCGGAACC TTTGCATCAA GGAAGGAGCC ACTGCCAAGT TTGAAGGGAG GGTCCGGGGT
    TACCCAGAGC CCCAGGTGAC ATGGCACAGA AACGGACAGC CCATCACCAG TGGAGGTCGC
    TTCCTGCTGG ACTGTGGCAT CCGGGGGACC TTCAGTCTTG TGATTCATTC TGTCTGTGAG
    GAGGACAAGG GAAAGTATAC CTGTGAAGCC ACCAATGGTA GTGGCGCCCG CCAGGTGACA
    GTGGAGTTGA CGGTAGAAGG GGGCTTTGTG AAGAAGCATG GCCAGCCTGT TGTTTCCAAA
    GCCTTGGGGG ACAGATTTGC AGCCCCTGCA GTCGAGACCC GTCCTAGCAT CTGGGGAGAG
    TGCCCACCAA AGTTTGCCAC CAAGCTGGGC CGAGCGGTGG TCAAAGAAGG ACAGATGGGG
    CGATTCTCCT GCAAGATCAC TGGCCGGCCC CAACCGCAGG TCACCTGGCT CAAGGGAGAT
    GTTCCCCTGC AGCCCAGTGC CCGTGTGACC ATGTCTGAGA AGAATGGGGT GCAAGTGCTG
    GAAATCCACG AAGTCAACCG AGACGACGTG GGGGTGTACA CGTGCATGGT GGTGAATGGG
    TCGGGGAAGG CCTCCATGTC AGCTGAGCTC TCCATCCAAG GTTTGGACAA TACCAATAGG
    TCGCTTGTGA GAGGAACAAA GGCGGCCAGT TCAGACATCA GGACAGAAGC GACCAATGGA
    CTCTCACAGG GGCCAAAGCT GGATGGCCTG GAGGCTGCAG CGGAGAGTAA GAACTGCTCC
    AGCGCCCAGA GGGGTGGCTC CCCGACCTGG GCTTCAGGGA GCCAGCCTCA GCCTCCGAGG
    GAATCCAAGC TGGAGCCGTC AGGGGGCTCA GACAGAAAAG CCCTGAGGAG CCCCATCCTG
    CAGAAGACTT CCAGTGCCAT CACCCTGCAG CCTGCAAAAG TCCAGTTGGA ACCAAGGGCT
    CCAGTCTCAG GCACCCTCTT GCCTTCCAGA GAAGAGAGAG AGCGGCCAGC TGCTCCACCT
    GCCGCCACCC TGCCCTCCAG ACAGTCTGGC ACAGGAGGCC AAGAAGCTGT GAGCAAGGCT
    GCTACCAAGC AGGTTGCCAT GGAGAGCCGA AGGGCTGCCA CAGTCCCCAG ATTCGAGAGC
    AAGCCCCAAA GCCAGGAGGT CAGTGAAGAT CAGCCAGTCA AGTTCAAGTG TGAAGTTTCA
    GGGGTTCCAA AGCCCGAAGT GACCTGGTTC CTGGACGGTG CCCCTGTGAA GAGGCGAGAA
    GGCACCTTTG AGGTTTATGA AGCCGGAGGA TCCCACTACC TCTGCCTGCC AAGAGCCCGG
    GCCAGGGACA GCGGGAACTA CAGCTGCACA GCCACCAACA TCCGAGGCCA GGTGTCCTGT
    ACCTGGACCC TCCTGGTGAA AAGGCTGGCC GTGACAGAGG CGCCCCCGTC CTTCTCCAGT
    GTCCTGAAGG ACTGTGCTGT GGTTGAGGGC CAGGACTTCG TGCTGCAGTG CTCCGTGCAG
    GGGACCCCGC TGCCCCAGAT CACTTGGCTG CTCAATGGGC AGCCCATCCA GTACGCCCGC
    TCCACCTGCG AGGCCGGCGT TGCCAAGCTC CACATCCAGG ATGCCCTCCC GGAGGACGAC
    GGCACCTATA CCTGTCTGGC CGAGAACACC TTGGGGCGGG TGTCCTGCAG CGCCCGGGTC
    ACTGTCCATG AAAAGAAGAG TGATGGGAAG AGTGAGTACC TCCTGCCCGC TGCCCCCAGC
    AAGCCCATGG CACCCATCTT CCTGCAGGGT CTCTCTGATC TCAGCGTCAT GGATGGGAGC
    CAGGTCACCA TGACAGTCCA GGTGTCAGGG AACCCACCCC CTGAGGTCAT CTGGCTTCAC
    GATGGGAATG AGATCCAGGA GTCGGAGGAC TTCCACTTTG AGCAGAGGGG CACTCGGCAC
    AGCCTCTGTA TCCAGGAAGT GTTCCCAGAA GACACAGGCA CATACACCTG TGAAGCCTGG
    AACAGCGCAG GAGAGGTCCG CACCCAGGCC GTGCTCACGG TGCAAGAGCC TCAGGACGGC
    ACCCAGCCTT GGTTCATCAG CAAGCCCCGC TCTGTGACAG CCTGCCTGGG CCAGAGTGTC
    CTCATCTCCT GTGCCATAGC TGGGGACCCC TTCCCTACTG TGCACTGGCT CCGAGACGGC
    AAAGCCCTCT CCAAAGACAC TGGCCACTTC GAGGTGCTGC AGAATGAGGA CGTGTTCACC
    CTGGTTCTCA AGAACGTGCA GCCCTGGCAC GCCGGCCAGT ATGAGATCCT GCTCAAGAAC
    CGGGTTGGCG AGTGCAGCTG CCAGGTGTCC CTGGTGCTAC AGAGCAGCCC TGCCAGAGCC
    CCCCTGCGGG GGAGGGAGCC TGCCAGCTGT GAGGGCCAAG GAGCTGGTGC TGATGGTGGT
    GGTGCTGGCA GAGACGGCTA CGGGAGCCTG AGGCCCGGCT GGCCAGCAAG AGGGCAGGGT
    GAGCCCGAGG ACGAAGACGG CGAGGACGTG CGCGGGGTGC TCAAGAGGCG CGTGGAGACG
    CGGCAGCACA CCGAGGAGGC TATCCGCCAG CAGGAGGTGG AGCAGCTGGA CTTCCGCGAC
    CTCCTGGGCA AGAAGGTGAG CACCAAGACC TTGTCTGAGG AGGACCTGAA GGAGATCCCT
    GCCGAGCAGA TGGATTTCCG CGCCAATCTG CAGCGGCAAG TCAAGCCGAA GACTGTGTCC
    GAGGAAGAGA GGAAGGTGCA TAGCCCCCAG CAGGTGGACT TCCGCTCGGT CCTGGCCAAG
    AAGGGGACGC CCAAGACCCC CGTACCTGAG AAGCTGCCCC CTCCAAAACC TGCCACCCCA
    GATTTTCGCT CGGTGTTGGG CAGTAAGAAA AAATTACCAG CGGAGAATGG TAGCAGCAAT
    GCTGAGCCTT TGAATGTCAA GGCAGCAGAA GGTCCCAAGC CTGTGGGCAA TGCCCAGCCT
    TCAGGCTTTC TCAAACCTAT GGGCAATGCC AAGCTGGCCG AGACCTCCAA ACACTTGAGT
    AATGCCAAGC CAGCCGAGCT CCCCAAACCT GTGGGCAACG CCAAGCCAGC CGAGATCCCC
    AAACCCGTGG GCAACACCAG GCCAGCTGAG ACCCCCAAAA CCGTGGGCAA TGCCAAGCCA
    ACTGAGACCT TCAAACCCAT GGCCAATACC AAGCCAGCTG AGACCCTAAA GCCTGTGGGC
    AATGCCAAGC CAGCTGAGAC CCTAAAGCCT GTGGGCAATG CCAAGCCAGC TGAGACCCTA
    AAGCCCGTGG GCAATGCCAA GCCAGCTGAG ACCCCCAAGC CCTTGGGTAA TACCAAGCCA
    GCCGAGCCCC CTAAACCAGC TGGGAAAGAA GAGCTTAAGA AGGAGGTTAA GAATGATGTG
    AACTACAAGA GGGGCTCTGC AGGGGCCACA GATAATGAAA AAAGCCCGGA GAGCCAAGGG
    TCAGCCCCAA CCTTCAAGGA AAAGCTACGT GATCTCCGTG TGGCAGAGGG TGAGAAGCTG
    CTGCTCCAGT GCCAGGTATC CTCCGACCCC CCGGCCACCA TCACCTGGAC ACTGAATGGG
    AAGACACTCA AGACCACCAA GTTCATTGTC CTCTCCCAAG AAGGCTCACT GTGCTCCATC
    TCCATCGAGA AGGCCTTGCC CGAGGACAGA GGTTTGTACA AGTGTGTGGC CAAGAACAGC
    GCCGGCCAGG CTGAGTGCTC CTGCCAAGTC ACCGTGGACG ATGCCCCCAC CGGAGAGAAT
    GCCAAGGCCC CGGAGATGAA AGCCCGGAGG CCAAAGAGCT CTCTTCCTCC TGTGCTAGGA
    ACAGAGAGTG ATGCCACTGT GAAAAAGAAA CCTGCCCCCA AGACACCTCC GAAGGCAGCC
    ATGCCCCCTC AGATCATCCA GTTTCCCGAG GACCAAAAGG TGCGAGCGGG AGAGTCTGTG
    GAGCTGTTTG GTAAAGTGGC CGGTACCCAG CCCATCACCT GTACCTGGAT GAAGTTCCGA
    AAGCAGATCC AGGAGAGTGA GCACATCAAG GTGGAGAACA GTGAGAATGG CAGCAAGCTC
    ACCATCCGGG CCGCGCGCCA GGAGCACTGT GGCTGCTACA CTCTGCTGGT GGAGAACAAG
    CTGGGCAGCA GGCAGGCCCA GGTCAACCTC ACTGTTGTGG ACAAGCCGGA CCCCCCCGCA
    GGCACGCCTT GTGCCTCTGA CATCCGCAGC TCCTCGCTGA CCCTGTCCTG GTATGGCTCC
    TCCTACGATG GGGGCAGCGC CGTACAGTCC TACAGCGTCG AGATCTGGGA CTCAGTGGAC
    AAGACGTGGA AGGAACTAGC CACATGCCGC AGCACCTCTT TTAACGTCCA GGACCTGCTG
    TCTGACCGGG AGTATAAATT CCGTGTGCGT GCAATCAACA TCTACGGAAC CAGTGAGCCG
    AGCCAGGAGT CTGAACTCAC AGCGGTGGGA GAGAAACCTG AGGAGCCCAA GGATGAAGTG
    GAGGTGTCAG ATGACGATGA GAAGGAGCCT GAGGTCGATT ACCGGACCGT CACGGTCAAT
    ACGGAACAAA AAGTGTCTGA CTTCTACGAC ATTGAAGAGA GACTAGGATC TGGGAAATTT
    GGACAGGTCT TCCGACTTGT AGAGAAGAAA ACTGGAAAAA TCTGGGCAGG GAAATTCTTC
    AAGGCGTATT CAGCAAAAGA GAAAGAGAAC ATCCGGCAGG AGATCAGCAT CATGAACTGC
    CTCCACCACC CCAAGCTGGT CCAGTGTGTG GACGCCTTCG AAGAGAAGGC CAACATCGTC
    ATGGTCCTGG AGATCGTGTC CGGGGGCGAG CTGTTCGAGC GCATCATTGA CGAGGACTTT
    GAGCTGACGG AGCGCGAGTG CATCAAGTAC ATGCGGCAGA TCTCGGAGGG GGTGGAGTAC
    ATCCACAAGC AGGGCATCGT CCACCTGGAC CTCAAGCCTG AGAACATCAT GTGTGTCAAC
    AAGACGGGCA CCCGGATCAA GCTCATCGAC TTCGGTCTGG CCCGGCGGCT GGAGAATGCG
    GGGTCTCTGA AGGTCCTCTT TGGCACCCCG GAGTTTGTGG CGCCTGAAGT GATCAACTAT
    GAGCCCATTG GCTATGCCAC GGACATGTGG AGCATCGGGG TCATCTGCTA CATCCTGGTC
    AGTGGACTGT CCCCCTTTAT GGGTGACAAC GATAACGAGA CCTTAGCCAA CGTTACCTCA
    GCCACCTGGG ACTTCGACGA TGAGGCCTTC GATGAGATCT CCGACGACGC CAAGGATTTC
    ATCAGCAATT TACTGAAGAA AGATATGAAA AACCGCTTGG ACTGCACCCA GTGCCTCCAG
    CACCCATGGC TAATGAAAGA TACCAAGAAC ATGGAGGCCA AGAAGCTCTC CAAGGACCGG
    ATGAAAAAGT ACATGGCAAG GAGGAAATGG CAGAAAACGG GCAATGCTGT GAGAGCCATT
    GGAAGACTGT CCTCTATGGC AATGATCTCA GGGCTCAGTG GCAGGAAATC CTCAACAGGG
    TCACCAACCA GCCCGCTGAA TGCAGAAAAA TTAGAATCTG AAGAAGATGT CTCCCAAGCT
    TTCCTTGAGG CCGTCGCTGA GGAAAAGCCC CATGTAAAAC CCTATTTTTC TAAGACCATT
    CGTGATTTAG AAGTTGTGGA GGGAAGCGCT GCTAGATTTG ACTGCAAGAT TGAAGGATAC
    CCAGACCCTG AGGTTGTCTG GTTCAAAGAT GACCAGTCAA TCAGGGAGTC CCGCCACTTC
    CAGATAGACT ACGATGAGGA TGGGAACTGC TCTTTAATCA TTAGCGATGT CTGCGGTGAT
    GATGATGCCA AGTACACCTG CAAGGCTGTC AACAGTCTTG GAGAAGCCAC CTGCACAGCA
    GAGCTCATTG TGGAAACCAT GGAGGAAGGA GAAGGGGAAG GGGAAGAAGA AGAGGAGGAA
    GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe236095
    Clone ID Related Accession (Same CDS sequence) XM_021070232.1 , XM_021070231.1 , XM_021070234.1 , XM_021070235.1 , XM_021070233.1
    Accession Version XM_021070235.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5946bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product myosin light chain kinase, smooth muscle isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010455.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGATCACT CGAGAGTCGG CTCCACGCCC CTGACCGAGG CCCCTGCTTT CATTTTGCCC 
    CCTCGGAACC TTTGCATCAA GGAAGGAGCC ACTGCCAAGT TTGAAGGGAG GGTCCGGGGT
    TACCCAGAGC CCCAGGTGAC ATGGCACAGA AACGGACAGC CCATCACCAG TGGAGGTCGC
    TTCCTGCTGG ACTGTGGCAT CCGGGGGACC TTCAGTCTTG TGATTCATTC TGTCTGTGAG
    GAGGACAAGG GAAAGTATAC CTGTGAAGCC ACCAATGGTA GTGGCGCCCG CCAGGTGACA
    GTGGAGTTGA CGGTAGAAGG GGGCTTTGTG AAGAAGCATG GCCAGCCTGT TGTTTCCAAA
    GCCTTGGGGG ACAGATTTGC AGCCCCTGCA GTCGAGACCC GTCCTAGCAT CTGGGGAGAG
    TGCCCACCAA AGTTTGCCAC CAAGCTGGGC CGAGCGGTGG TCAAAGAAGG ACAGATGGGG
    CGATTCTCCT GCAAGATCAC TGGCCGGCCC CAACCGCAGG TCACCTGGCT CAAGGGAGAT
    GTTCCCCTGC AGCCCAGTGC CCGTGTGACC ATGTCTGAGA AGAATGGGGT GCAAGTGCTG
    GAAATCCACG AAGTCAACCG AGACGACGTG GGGGTGTACA CGTGCATGGT GGTGAATGGG
    TCGGGGAAGG CCTCCATGTC AGCTGAGCTC TCCATCCAAG GTTTGGACAA TACCAATAGG
    TCGCTTGTGA GAGGAACAAA GGCGGCCAGT TCAGACATCA GGACAGAAGC GACCAATGGA
    CTCTCACAGG GGCCAAAGCT GGATGGCCTG GAGGCTGCAG CGGAGAGTAA GAACTGCTCC
    AGCGCCCAGA GGGGTGGCTC CCCGACCTGG GCTTCAGGGA GCCAGCCTCA GCCTCCGAGG
    GAATCCAAGC TGGAGCCGTC AGGGGGCTCA GACAGAAAAG CCCTGAGGAG CCCCATCCTG
    CAGAAGACTT CCAGTGCCAT CACCCTGCAG CCTGCAAAAG TCCAGTTGGA ACCAAGGGCT
    CCAGTCTCAG GCACCCTCTT GCCTTCCAGA GAAGAGAGAG AGCGGCCAGC TGCTCCACCT
    GCCGCCACCC TGCCCTCCAG ACAGTCTGGC ACAGGAGGCC AAGAAGCTGT GAGCAAGGCT
    GCTACCAAGC AGGTTGCCAT GGAGAGCCGA AGGGCTGCCA CAGTCCCCAG ATTCGAGAGC
    AAGCCCCAAA GCCAGGAGGT CAGTGAAGAT CAGCCAGTCA AGTTCAAGTG TGAAGTTTCA
    GGGGTTCCAA AGCCCGAAGT GACCTGGTTC CTGGACGGTG CCCCTGTGAA GAGGCGAGAA
    GGCACCTTTG AGGTTTATGA AGCCGGAGGA TCCCACTACC TCTGCCTGCC AAGAGCCCGG
    GCCAGGGACA GCGGGAACTA CAGCTGCACA GCCACCAACA TCCGAGGCCA GGTGTCCTGT
    ACCTGGACCC TCCTGGTGAA AAGGCTGGCC GTGACAGAGG CGCCCCCGTC CTTCTCCAGT
    GTCCTGAAGG ACTGTGCTGT GGTTGAGGGC CAGGACTTCG TGCTGCAGTG CTCCGTGCAG
    GGGACCCCGC TGCCCCAGAT CACTTGGCTG CTCAATGGGC AGCCCATCCA GTACGCCCGC
    TCCACCTGCG AGGCCGGCGT TGCCAAGCTC CACATCCAGG ATGCCCTCCC GGAGGACGAC
    GGCACCTATA CCTGTCTGGC CGAGAACACC TTGGGGCGGG TGTCCTGCAG CGCCCGGGTC
    ACTGTCCATG AAAAGAAGAG TGATGGGAAG AGTGAGTACC TCCTGCCCGC TGCCCCCAGC
    AAGCCCATGG CACCCATCTT CCTGCAGGGT CTCTCTGATC TCAGCGTCAT GGATGGGAGC
    CAGGTCACCA TGACAGTCCA GGTGTCAGGG AACCCACCCC CTGAGGTCAT CTGGCTTCAC
    GATGGGAATG AGATCCAGGA GTCGGAGGAC TTCCACTTTG AGCAGAGGGG CACTCGGCAC
    AGCCTCTGTA TCCAGGAAGT GTTCCCAGAA GACACAGGCA CATACACCTG TGAAGCCTGG
    AACAGCGCAG GAGAGGTCCG CACCCAGGCC GTGCTCACGG TGCAAGAGCC TCAGGACGGC
    ACCCAGCCTT GGTTCATCAG CAAGCCCCGC TCTGTGACAG CCTGCCTGGG CCAGAGTGTC
    CTCATCTCCT GTGCCATAGC TGGGGACCCC TTCCCTACTG TGCACTGGCT CCGAGACGGC
    AAAGCCCTCT CCAAAGACAC TGGCCACTTC GAGGTGCTGC AGAATGAGGA CGTGTTCACC
    CTGGTTCTCA AGAACGTGCA GCCCTGGCAC GCCGGCCAGT ATGAGATCCT GCTCAAGAAC
    CGGGTTGGCG AGTGCAGCTG CCAGGTGTCC CTGGTGCTAC AGAGCAGCCC TGCCAGAGCC
    CCCCTGCGGG GGAGGGAGCC TGCCAGCTGT GAGGGCCAAG GAGCTGGTGC TGATGGTGGT
    GGTGCTGGCA GAGACGGCTA CGGGAGCCTG AGGCCCGGCT GGCCAGCAAG AGGGCAGGGT
    GAGCCCGAGG ACGAAGACGG CGAGGACGTG CGCGGGGTGC TCAAGAGGCG CGTGGAGACG
    CGGCAGCACA CCGAGGAGGC TATCCGCCAG CAGGAGGTGG AGCAGCTGGA CTTCCGCGAC
    CTCCTGGGCA AGAAGGTGAG CACCAAGACC TTGTCTGAGG AGGACCTGAA GGAGATCCCT
    GCCGAGCAGA TGGATTTCCG CGCCAATCTG CAGCGGCAAG TCAAGCCGAA GACTGTGTCC
    GAGGAAGAGA GGAAGGTGCA TAGCCCCCAG CAGGTGGACT TCCGCTCGGT CCTGGCCAAG
    AAGGGGACGC CCAAGACCCC CGTACCTGAG AAGCTGCCCC CTCCAAAACC TGCCACCCCA
    GATTTTCGCT CGGTGTTGGG CAGTAAGAAA AAATTACCAG CGGAGAATGG TAGCAGCAAT
    GCTGAGCCTT TGAATGTCAA GGCAGCAGAA GGTCCCAAGC CTGTGGGCAA TGCCCAGCCT
    TCAGGCTTTC TCAAACCTAT GGGCAATGCC AAGCTGGCCG AGACCTCCAA ACACTTGAGT
    AATGCCAAGC CAGCCGAGCT CCCCAAACCT GTGGGCAACG CCAAGCCAGC CGAGATCCCC
    AAACCCGTGG GCAACACCAG GCCAGCTGAG ACCCCCAAAA CCGTGGGCAA TGCCAAGCCA
    ACTGAGACCT TCAAACCCAT GGCCAATACC AAGCCAGCTG AGACCCTAAA GCCTGTGGGC
    AATGCCAAGC CAGCTGAGAC CCTAAAGCCT GTGGGCAATG CCAAGCCAGC TGAGACCCTA
    AAGCCCGTGG GCAATGCCAA GCCAGCTGAG ACCCCCAAGC CCTTGGGTAA TACCAAGCCA
    GCCGAGCCCC CTAAACCAGC TGGGAAAGAA GAGCTTAAGA AGGAGGTTAA GAATGATGTG
    AACTACAAGA GGGGCTCTGC AGGGGCCACA GATAATGAAA AAAGCCCGGA GAGCCAAGGG
    TCAGCCCCAA CCTTCAAGGA AAAGCTACGT GATCTCCGTG TGGCAGAGGG TGAGAAGCTG
    CTGCTCCAGT GCCAGGTATC CTCCGACCCC CCGGCCACCA TCACCTGGAC ACTGAATGGG
    AAGACACTCA AGACCACCAA GTTCATTGTC CTCTCCCAAG AAGGCTCACT GTGCTCCATC
    TCCATCGAGA AGGCCTTGCC CGAGGACAGA GGTTTGTACA AGTGTGTGGC CAAGAACAGC
    GCCGGCCAGG CTGAGTGCTC CTGCCAAGTC ACCGTGGACG ATGCCCCCAC CGGAGAGAAT
    GCCAAGGCCC CGGAGATGAA AGCCCGGAGG CCAAAGAGCT CTCTTCCTCC TGTGCTAGGA
    ACAGAGAGTG ATGCCACTGT GAAAAAGAAA CCTGCCCCCA AGACACCTCC GAAGGCAGCC
    ATGCCCCCTC AGATCATCCA GTTTCCCGAG GACCAAAAGG TGCGAGCGGG AGAGTCTGTG
    GAGCTGTTTG GTAAAGTGGC CGGTACCCAG CCCATCACCT GTACCTGGAT GAAGTTCCGA
    AAGCAGATCC AGGAGAGTGA GCACATCAAG GTGGAGAACA GTGAGAATGG CAGCAAGCTC
    ACCATCCGGG CCGCGCGCCA GGAGCACTGT GGCTGCTACA CTCTGCTGGT GGAGAACAAG
    CTGGGCAGCA GGCAGGCCCA GGTCAACCTC ACTGTTGTGG ACAAGCCGGA CCCCCCCGCA
    GGCACGCCTT GTGCCTCTGA CATCCGCAGC TCCTCGCTGA CCCTGTCCTG GTATGGCTCC
    TCCTACGATG GGGGCAGCGC CGTACAGTCC TACAGCGTCG AGATCTGGGA CTCAGTGGAC
    AAGACGTGGA AGGAACTAGC CACATGCCGC AGCACCTCTT TTAACGTCCA GGACCTGCTG
    TCTGACCGGG AGTATAAATT CCGTGTGCGT GCAATCAACA TCTACGGAAC CAGTGAGCCG
    AGCCAGGAGT CTGAACTCAC AGCGGTGGGA GAGAAACCTG AGGAGCCCAA GGATGAAGTG
    GAGGTGTCAG ATGACGATGA GAAGGAGCCT GAGGTCGATT ACCGGACCGT CACGGTCAAT
    ACGGAACAAA AAGTGTCTGA CTTCTACGAC ATTGAAGAGA GACTAGGATC TGGGAAATTT
    GGACAGGTCT TCCGACTTGT AGAGAAGAAA ACTGGAAAAA TCTGGGCAGG GAAATTCTTC
    AAGGCGTATT CAGCAAAAGA GAAAGAGAAC ATCCGGCAGG AGATCAGCAT CATGAACTGC
    CTCCACCACC CCAAGCTGGT CCAGTGTGTG GACGCCTTCG AAGAGAAGGC CAACATCGTC
    ATGGTCCTGG AGATCGTGTC CGGGGGCGAG CTGTTCGAGC GCATCATTGA CGAGGACTTT
    GAGCTGACGG AGCGCGAGTG CATCAAGTAC ATGCGGCAGA TCTCGGAGGG GGTGGAGTAC
    ATCCACAAGC AGGGCATCGT CCACCTGGAC CTCAAGCCTG AGAACATCAT GTGTGTCAAC
    AAGACGGGCA CCCGGATCAA GCTCATCGAC TTCGGTCTGG CCCGGCGGCT GGAGAATGCG
    GGGTCTCTGA AGGTCCTCTT TGGCACCCCG GAGTTTGTGG CGCCTGAAGT GATCAACTAT
    GAGCCCATTG GCTATGCCAC GGACATGTGG AGCATCGGGG TCATCTGCTA CATCCTGGTC
    AGTGGACTGT CCCCCTTTAT GGGTGACAAC GATAACGAGA CCTTAGCCAA CGTTACCTCA
    GCCACCTGGG ACTTCGACGA TGAGGCCTTC GATGAGATCT CCGACGACGC CAAGGATTTC
    ATCAGCAATT TACTGAAGAA AGATATGAAA AACCGCTTGG ACTGCACCCA GTGCCTCCAG
    CACCCATGGC TAATGAAAGA TACCAAGAAC ATGGAGGCCA AGAAGCTCTC CAAGGACCGG
    ATGAAAAAGT ACATGGCAAG GAGGAAATGG CAGAAAACGG GCAATGCTGT GAGAGCCATT
    GGAAGACTGT CCTCTATGGC AATGATCTCA GGGCTCAGTG GCAGGAAATC CTCAACAGGG
    TCACCAACCA GCCCGCTGAA TGCAGAAAAA TTAGAATCTG AAGAAGATGT CTCCCAAGCT
    TTCCTTGAGG CCGTCGCTGA GGAAAAGCCC CATGTAAAAC CCTATTTTTC TAAGACCATT
    CGTGATTTAG AAGTTGTGGA GGGAAGCGCT GCTAGATTTG ACTGCAAGAT TGAAGGATAC
    CCAGACCCTG AGGTTGTCTG GTTCAAAGAT GACCAGTCAA TCAGGGAGTC CCGCCACTTC
    CAGATAGACT ACGATGAGGA TGGGAACTGC TCTTTAATCA TTAGCGATGT CTGCGGTGAT
    GATGATGCCA AGTACACCTG CAAGGCTGTC AACAGTCTTG GAGAAGCCAC CTGCACAGCA
    GAGCTCATTG TGGAAACCAT GGAGGAAGGA GAAGGGGAAG GGGAAGAAGA AGAGGAGGAA
    GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020925894.1
    CDS297..6242
    Translation

    Target ORF information:

    RefSeq Version XM_021070235.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa myosin light chain kinase (MYLK), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021070235.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGATCACT CGAGAGTCGG CTCCACGCCC CTGACCGAGG CCCCTGCTTT CATTTTGCCC 
    CCTCGGAACC TTTGCATCAA GGAAGGAGCC ACTGCCAAGT TTGAAGGGAG GGTCCGGGGT
    TACCCAGAGC CCCAGGTGAC ATGGCACAGA AACGGACAGC CCATCACCAG TGGAGGTCGC
    TTCCTGCTGG ACTGTGGCAT CCGGGGGACC TTCAGTCTTG TGATTCATTC TGTCTGTGAG
    GAGGACAAGG GAAAGTATAC CTGTGAAGCC ACCAATGGTA GTGGCGCCCG CCAGGTGACA
    GTGGAGTTGA CGGTAGAAGG GGGCTTTGTG AAGAAGCATG GCCAGCCTGT TGTTTCCAAA
    GCCTTGGGGG ACAGATTTGC AGCCCCTGCA GTCGAGACCC GTCCTAGCAT CTGGGGAGAG
    TGCCCACCAA AGTTTGCCAC CAAGCTGGGC CGAGCGGTGG TCAAAGAAGG ACAGATGGGG
    CGATTCTCCT GCAAGATCAC TGGCCGGCCC CAACCGCAGG TCACCTGGCT CAAGGGAGAT
    GTTCCCCTGC AGCCCAGTGC CCGTGTGACC ATGTCTGAGA AGAATGGGGT GCAAGTGCTG
    GAAATCCACG AAGTCAACCG AGACGACGTG GGGGTGTACA CGTGCATGGT GGTGAATGGG
    TCGGGGAAGG CCTCCATGTC AGCTGAGCTC TCCATCCAAG GTTTGGACAA TACCAATAGG
    TCGCTTGTGA GAGGAACAAA GGCGGCCAGT TCAGACATCA GGACAGAAGC GACCAATGGA
    CTCTCACAGG GGCCAAAGCT GGATGGCCTG GAGGCTGCAG CGGAGAGTAA GAACTGCTCC
    AGCGCCCAGA GGGGTGGCTC CCCGACCTGG GCTTCAGGGA GCCAGCCTCA GCCTCCGAGG
    GAATCCAAGC TGGAGCCGTC AGGGGGCTCA GACAGAAAAG CCCTGAGGAG CCCCATCCTG
    CAGAAGACTT CCAGTGCCAT CACCCTGCAG CCTGCAAAAG TCCAGTTGGA ACCAAGGGCT
    CCAGTCTCAG GCACCCTCTT GCCTTCCAGA GAAGAGAGAG AGCGGCCAGC TGCTCCACCT
    GCCGCCACCC TGCCCTCCAG ACAGTCTGGC ACAGGAGGCC AAGAAGCTGT GAGCAAGGCT
    GCTACCAAGC AGGTTGCCAT GGAGAGCCGA AGGGCTGCCA CAGTCCCCAG ATTCGAGAGC
    AAGCCCCAAA GCCAGGAGGT CAGTGAAGAT CAGCCAGTCA AGTTCAAGTG TGAAGTTTCA
    GGGGTTCCAA AGCCCGAAGT GACCTGGTTC CTGGACGGTG CCCCTGTGAA GAGGCGAGAA
    GGCACCTTTG AGGTTTATGA AGCCGGAGGA TCCCACTACC TCTGCCTGCC AAGAGCCCGG
    GCCAGGGACA GCGGGAACTA CAGCTGCACA GCCACCAACA TCCGAGGCCA GGTGTCCTGT
    ACCTGGACCC TCCTGGTGAA AAGGCTGGCC GTGACAGAGG CGCCCCCGTC CTTCTCCAGT
    GTCCTGAAGG ACTGTGCTGT GGTTGAGGGC CAGGACTTCG TGCTGCAGTG CTCCGTGCAG
    GGGACCCCGC TGCCCCAGAT CACTTGGCTG CTCAATGGGC AGCCCATCCA GTACGCCCGC
    TCCACCTGCG AGGCCGGCGT TGCCAAGCTC CACATCCAGG ATGCCCTCCC GGAGGACGAC
    GGCACCTATA CCTGTCTGGC CGAGAACACC TTGGGGCGGG TGTCCTGCAG CGCCCGGGTC
    ACTGTCCATG AAAAGAAGAG TGATGGGAAG AGTGAGTACC TCCTGCCCGC TGCCCCCAGC
    AAGCCCATGG CACCCATCTT CCTGCAGGGT CTCTCTGATC TCAGCGTCAT GGATGGGAGC
    CAGGTCACCA TGACAGTCCA GGTGTCAGGG AACCCACCCC CTGAGGTCAT CTGGCTTCAC
    GATGGGAATG AGATCCAGGA GTCGGAGGAC TTCCACTTTG AGCAGAGGGG CACTCGGCAC
    AGCCTCTGTA TCCAGGAAGT GTTCCCAGAA GACACAGGCA CATACACCTG TGAAGCCTGG
    AACAGCGCAG GAGAGGTCCG CACCCAGGCC GTGCTCACGG TGCAAGAGCC TCAGGACGGC
    ACCCAGCCTT GGTTCATCAG CAAGCCCCGC TCTGTGACAG CCTGCCTGGG CCAGAGTGTC
    CTCATCTCCT GTGCCATAGC TGGGGACCCC TTCCCTACTG TGCACTGGCT CCGAGACGGC
    AAAGCCCTCT CCAAAGACAC TGGCCACTTC GAGGTGCTGC AGAATGAGGA CGTGTTCACC
    CTGGTTCTCA AGAACGTGCA GCCCTGGCAC GCCGGCCAGT ATGAGATCCT GCTCAAGAAC
    CGGGTTGGCG AGTGCAGCTG CCAGGTGTCC CTGGTGCTAC AGAGCAGCCC TGCCAGAGCC
    CCCCTGCGGG GGAGGGAGCC TGCCAGCTGT GAGGGCCAAG GAGCTGGTGC TGATGGTGGT
    GGTGCTGGCA GAGACGGCTA CGGGAGCCTG AGGCCCGGCT GGCCAGCAAG AGGGCAGGGT
    GAGCCCGAGG ACGAAGACGG CGAGGACGTG CGCGGGGTGC TCAAGAGGCG CGTGGAGACG
    CGGCAGCACA CCGAGGAGGC TATCCGCCAG CAGGAGGTGG AGCAGCTGGA CTTCCGCGAC
    CTCCTGGGCA AGAAGGTGAG CACCAAGACC TTGTCTGAGG AGGACCTGAA GGAGATCCCT
    GCCGAGCAGA TGGATTTCCG CGCCAATCTG CAGCGGCAAG TCAAGCCGAA GACTGTGTCC
    GAGGAAGAGA GGAAGGTGCA TAGCCCCCAG CAGGTGGACT TCCGCTCGGT CCTGGCCAAG
    AAGGGGACGC CCAAGACCCC CGTACCTGAG AAGCTGCCCC CTCCAAAACC TGCCACCCCA
    GATTTTCGCT CGGTGTTGGG CAGTAAGAAA AAATTACCAG CGGAGAATGG TAGCAGCAAT
    GCTGAGCCTT TGAATGTCAA GGCAGCAGAA GGTCCCAAGC CTGTGGGCAA TGCCCAGCCT
    TCAGGCTTTC TCAAACCTAT GGGCAATGCC AAGCTGGCCG AGACCTCCAA ACACTTGAGT
    AATGCCAAGC CAGCCGAGCT CCCCAAACCT GTGGGCAACG CCAAGCCAGC CGAGATCCCC
    AAACCCGTGG GCAACACCAG GCCAGCTGAG ACCCCCAAAA CCGTGGGCAA TGCCAAGCCA
    ACTGAGACCT TCAAACCCAT GGCCAATACC AAGCCAGCTG AGACCCTAAA GCCTGTGGGC
    AATGCCAAGC CAGCTGAGAC CCTAAAGCCT GTGGGCAATG CCAAGCCAGC TGAGACCCTA
    AAGCCCGTGG GCAATGCCAA GCCAGCTGAG ACCCCCAAGC CCTTGGGTAA TACCAAGCCA
    GCCGAGCCCC CTAAACCAGC TGGGAAAGAA GAGCTTAAGA AGGAGGTTAA GAATGATGTG
    AACTACAAGA GGGGCTCTGC AGGGGCCACA GATAATGAAA AAAGCCCGGA GAGCCAAGGG
    TCAGCCCCAA CCTTCAAGGA AAAGCTACGT GATCTCCGTG TGGCAGAGGG TGAGAAGCTG
    CTGCTCCAGT GCCAGGTATC CTCCGACCCC CCGGCCACCA TCACCTGGAC ACTGAATGGG
    AAGACACTCA AGACCACCAA GTTCATTGTC CTCTCCCAAG AAGGCTCACT GTGCTCCATC
    TCCATCGAGA AGGCCTTGCC CGAGGACAGA GGTTTGTACA AGTGTGTGGC CAAGAACAGC
    GCCGGCCAGG CTGAGTGCTC CTGCCAAGTC ACCGTGGACG ATGCCCCCAC CGGAGAGAAT
    GCCAAGGCCC CGGAGATGAA AGCCCGGAGG CCAAAGAGCT CTCTTCCTCC TGTGCTAGGA
    ACAGAGAGTG ATGCCACTGT GAAAAAGAAA CCTGCCCCCA AGACACCTCC GAAGGCAGCC
    ATGCCCCCTC AGATCATCCA GTTTCCCGAG GACCAAAAGG TGCGAGCGGG AGAGTCTGTG
    GAGCTGTTTG GTAAAGTGGC CGGTACCCAG CCCATCACCT GTACCTGGAT GAAGTTCCGA
    AAGCAGATCC AGGAGAGTGA GCACATCAAG GTGGAGAACA GTGAGAATGG CAGCAAGCTC
    ACCATCCGGG CCGCGCGCCA GGAGCACTGT GGCTGCTACA CTCTGCTGGT GGAGAACAAG
    CTGGGCAGCA GGCAGGCCCA GGTCAACCTC ACTGTTGTGG ACAAGCCGGA CCCCCCCGCA
    GGCACGCCTT GTGCCTCTGA CATCCGCAGC TCCTCGCTGA CCCTGTCCTG GTATGGCTCC
    TCCTACGATG GGGGCAGCGC CGTACAGTCC TACAGCGTCG AGATCTGGGA CTCAGTGGAC
    AAGACGTGGA AGGAACTAGC CACATGCCGC AGCACCTCTT TTAACGTCCA GGACCTGCTG
    TCTGACCGGG AGTATAAATT CCGTGTGCGT GCAATCAACA TCTACGGAAC CAGTGAGCCG
    AGCCAGGAGT CTGAACTCAC AGCGGTGGGA GAGAAACCTG AGGAGCCCAA GGATGAAGTG
    GAGGTGTCAG ATGACGATGA GAAGGAGCCT GAGGTCGATT ACCGGACCGT CACGGTCAAT
    ACGGAACAAA AAGTGTCTGA CTTCTACGAC ATTGAAGAGA GACTAGGATC TGGGAAATTT
    GGACAGGTCT TCCGACTTGT AGAGAAGAAA ACTGGAAAAA TCTGGGCAGG GAAATTCTTC
    AAGGCGTATT CAGCAAAAGA GAAAGAGAAC ATCCGGCAGG AGATCAGCAT CATGAACTGC
    CTCCACCACC CCAAGCTGGT CCAGTGTGTG GACGCCTTCG AAGAGAAGGC CAACATCGTC
    ATGGTCCTGG AGATCGTGTC CGGGGGCGAG CTGTTCGAGC GCATCATTGA CGAGGACTTT
    GAGCTGACGG AGCGCGAGTG CATCAAGTAC ATGCGGCAGA TCTCGGAGGG GGTGGAGTAC
    ATCCACAAGC AGGGCATCGT CCACCTGGAC CTCAAGCCTG AGAACATCAT GTGTGTCAAC
    AAGACGGGCA CCCGGATCAA GCTCATCGAC TTCGGTCTGG CCCGGCGGCT GGAGAATGCG
    GGGTCTCTGA AGGTCCTCTT TGGCACCCCG GAGTTTGTGG CGCCTGAAGT GATCAACTAT
    GAGCCCATTG GCTATGCCAC GGACATGTGG AGCATCGGGG TCATCTGCTA CATCCTGGTC
    AGTGGACTGT CCCCCTTTAT GGGTGACAAC GATAACGAGA CCTTAGCCAA CGTTACCTCA
    GCCACCTGGG ACTTCGACGA TGAGGCCTTC GATGAGATCT CCGACGACGC CAAGGATTTC
    ATCAGCAATT TACTGAAGAA AGATATGAAA AACCGCTTGG ACTGCACCCA GTGCCTCCAG
    CACCCATGGC TAATGAAAGA TACCAAGAAC ATGGAGGCCA AGAAGCTCTC CAAGGACCGG
    ATGAAAAAGT ACATGGCAAG GAGGAAATGG CAGAAAACGG GCAATGCTGT GAGAGCCATT
    GGAAGACTGT CCTCTATGGC AATGATCTCA GGGCTCAGTG GCAGGAAATC CTCAACAGGG
    TCACCAACCA GCCCGCTGAA TGCAGAAAAA TTAGAATCTG AAGAAGATGT CTCCCAAGCT
    TTCCTTGAGG CCGTCGCTGA GGAAAAGCCC CATGTAAAAC CCTATTTTTC TAAGACCATT
    CGTGATTTAG AAGTTGTGGA GGGAAGCGCT GCTAGATTTG ACTGCAAGAT TGAAGGATAC
    CCAGACCCTG AGGTTGTCTG GTTCAAAGAT GACCAGTCAA TCAGGGAGTC CCGCCACTTC
    CAGATAGACT ACGATGAGGA TGGGAACTGC TCTTTAATCA TTAGCGATGT CTGCGGTGAT
    GATGATGCCA AGTACACCTG CAAGGCTGTC AACAGTCTTG GAGAAGCCAC CTGCACAGCA
    GAGCTCATTG TGGAAACCAT GGAGGAAGGA GAAGGGGAAG GGGAAGAAGA AGAGGAGGAA
    GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe236095
    Clone ID Related Accession (Same CDS sequence) XM_021070232.1 , XM_021070231.1 , XM_021070234.1 , XM_021070235.1 , XM_021070233.1
    Accession Version XM_021070233.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5946bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product myosin light chain kinase, smooth muscle isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010455.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGATCACT CGAGAGTCGG CTCCACGCCC CTGACCGAGG CCCCTGCTTT CATTTTGCCC 
    CCTCGGAACC TTTGCATCAA GGAAGGAGCC ACTGCCAAGT TTGAAGGGAG GGTCCGGGGT
    TACCCAGAGC CCCAGGTGAC ATGGCACAGA AACGGACAGC CCATCACCAG TGGAGGTCGC
    TTCCTGCTGG ACTGTGGCAT CCGGGGGACC TTCAGTCTTG TGATTCATTC TGTCTGTGAG
    GAGGACAAGG GAAAGTATAC CTGTGAAGCC ACCAATGGTA GTGGCGCCCG CCAGGTGACA
    GTGGAGTTGA CGGTAGAAGG GGGCTTTGTG AAGAAGCATG GCCAGCCTGT TGTTTCCAAA
    GCCTTGGGGG ACAGATTTGC AGCCCCTGCA GTCGAGACCC GTCCTAGCAT CTGGGGAGAG
    TGCCCACCAA AGTTTGCCAC CAAGCTGGGC CGAGCGGTGG TCAAAGAAGG ACAGATGGGG
    CGATTCTCCT GCAAGATCAC TGGCCGGCCC CAACCGCAGG TCACCTGGCT CAAGGGAGAT
    GTTCCCCTGC AGCCCAGTGC CCGTGTGACC ATGTCTGAGA AGAATGGGGT GCAAGTGCTG
    GAAATCCACG AAGTCAACCG AGACGACGTG GGGGTGTACA CGTGCATGGT GGTGAATGGG
    TCGGGGAAGG CCTCCATGTC AGCTGAGCTC TCCATCCAAG GTTTGGACAA TACCAATAGG
    TCGCTTGTGA GAGGAACAAA GGCGGCCAGT TCAGACATCA GGACAGAAGC GACCAATGGA
    CTCTCACAGG GGCCAAAGCT GGATGGCCTG GAGGCTGCAG CGGAGAGTAA GAACTGCTCC
    AGCGCCCAGA GGGGTGGCTC CCCGACCTGG GCTTCAGGGA GCCAGCCTCA GCCTCCGAGG
    GAATCCAAGC TGGAGCCGTC AGGGGGCTCA GACAGAAAAG CCCTGAGGAG CCCCATCCTG
    CAGAAGACTT CCAGTGCCAT CACCCTGCAG CCTGCAAAAG TCCAGTTGGA ACCAAGGGCT
    CCAGTCTCAG GCACCCTCTT GCCTTCCAGA GAAGAGAGAG AGCGGCCAGC TGCTCCACCT
    GCCGCCACCC TGCCCTCCAG ACAGTCTGGC ACAGGAGGCC AAGAAGCTGT GAGCAAGGCT
    GCTACCAAGC AGGTTGCCAT GGAGAGCCGA AGGGCTGCCA CAGTCCCCAG ATTCGAGAGC
    AAGCCCCAAA GCCAGGAGGT CAGTGAAGAT CAGCCAGTCA AGTTCAAGTG TGAAGTTTCA
    GGGGTTCCAA AGCCCGAAGT GACCTGGTTC CTGGACGGTG CCCCTGTGAA GAGGCGAGAA
    GGCACCTTTG AGGTTTATGA AGCCGGAGGA TCCCACTACC TCTGCCTGCC AAGAGCCCGG
    GCCAGGGACA GCGGGAACTA CAGCTGCACA GCCACCAACA TCCGAGGCCA GGTGTCCTGT
    ACCTGGACCC TCCTGGTGAA AAGGCTGGCC GTGACAGAGG CGCCCCCGTC CTTCTCCAGT
    GTCCTGAAGG ACTGTGCTGT GGTTGAGGGC CAGGACTTCG TGCTGCAGTG CTCCGTGCAG
    GGGACCCCGC TGCCCCAGAT CACTTGGCTG CTCAATGGGC AGCCCATCCA GTACGCCCGC
    TCCACCTGCG AGGCCGGCGT TGCCAAGCTC CACATCCAGG ATGCCCTCCC GGAGGACGAC
    GGCACCTATA CCTGTCTGGC CGAGAACACC TTGGGGCGGG TGTCCTGCAG CGCCCGGGTC
    ACTGTCCATG AAAAGAAGAG TGATGGGAAG AGTGAGTACC TCCTGCCCGC TGCCCCCAGC
    AAGCCCATGG CACCCATCTT CCTGCAGGGT CTCTCTGATC TCAGCGTCAT GGATGGGAGC
    CAGGTCACCA TGACAGTCCA GGTGTCAGGG AACCCACCCC CTGAGGTCAT CTGGCTTCAC
    GATGGGAATG AGATCCAGGA GTCGGAGGAC TTCCACTTTG AGCAGAGGGG CACTCGGCAC
    AGCCTCTGTA TCCAGGAAGT GTTCCCAGAA GACACAGGCA CATACACCTG TGAAGCCTGG
    AACAGCGCAG GAGAGGTCCG CACCCAGGCC GTGCTCACGG TGCAAGAGCC TCAGGACGGC
    ACCCAGCCTT GGTTCATCAG CAAGCCCCGC TCTGTGACAG CCTGCCTGGG CCAGAGTGTC
    CTCATCTCCT GTGCCATAGC TGGGGACCCC TTCCCTACTG TGCACTGGCT CCGAGACGGC
    AAAGCCCTCT CCAAAGACAC TGGCCACTTC GAGGTGCTGC AGAATGAGGA CGTGTTCACC
    CTGGTTCTCA AGAACGTGCA GCCCTGGCAC GCCGGCCAGT ATGAGATCCT GCTCAAGAAC
    CGGGTTGGCG AGTGCAGCTG CCAGGTGTCC CTGGTGCTAC AGAGCAGCCC TGCCAGAGCC
    CCCCTGCGGG GGAGGGAGCC TGCCAGCTGT GAGGGCCAAG GAGCTGGTGC TGATGGTGGT
    GGTGCTGGCA GAGACGGCTA CGGGAGCCTG AGGCCCGGCT GGCCAGCAAG AGGGCAGGGT
    GAGCCCGAGG ACGAAGACGG CGAGGACGTG CGCGGGGTGC TCAAGAGGCG CGTGGAGACG
    CGGCAGCACA CCGAGGAGGC TATCCGCCAG CAGGAGGTGG AGCAGCTGGA CTTCCGCGAC
    CTCCTGGGCA AGAAGGTGAG CACCAAGACC TTGTCTGAGG AGGACCTGAA GGAGATCCCT
    GCCGAGCAGA TGGATTTCCG CGCCAATCTG CAGCGGCAAG TCAAGCCGAA GACTGTGTCC
    GAGGAAGAGA GGAAGGTGCA TAGCCCCCAG CAGGTGGACT TCCGCTCGGT CCTGGCCAAG
    AAGGGGACGC CCAAGACCCC CGTACCTGAG AAGCTGCCCC CTCCAAAACC TGCCACCCCA
    GATTTTCGCT CGGTGTTGGG CAGTAAGAAA AAATTACCAG CGGAGAATGG TAGCAGCAAT
    GCTGAGCCTT TGAATGTCAA GGCAGCAGAA GGTCCCAAGC CTGTGGGCAA TGCCCAGCCT
    TCAGGCTTTC TCAAACCTAT GGGCAATGCC AAGCTGGCCG AGACCTCCAA ACACTTGAGT
    AATGCCAAGC CAGCCGAGCT CCCCAAACCT GTGGGCAACG CCAAGCCAGC CGAGATCCCC
    AAACCCGTGG GCAACACCAG GCCAGCTGAG ACCCCCAAAA CCGTGGGCAA TGCCAAGCCA
    ACTGAGACCT TCAAACCCAT GGCCAATACC AAGCCAGCTG AGACCCTAAA GCCTGTGGGC
    AATGCCAAGC CAGCTGAGAC CCTAAAGCCT GTGGGCAATG CCAAGCCAGC TGAGACCCTA
    AAGCCCGTGG GCAATGCCAA GCCAGCTGAG ACCCCCAAGC CCTTGGGTAA TACCAAGCCA
    GCCGAGCCCC CTAAACCAGC TGGGAAAGAA GAGCTTAAGA AGGAGGTTAA GAATGATGTG
    AACTACAAGA GGGGCTCTGC AGGGGCCACA GATAATGAAA AAAGCCCGGA GAGCCAAGGG
    TCAGCCCCAA CCTTCAAGGA AAAGCTACGT GATCTCCGTG TGGCAGAGGG TGAGAAGCTG
    CTGCTCCAGT GCCAGGTATC CTCCGACCCC CCGGCCACCA TCACCTGGAC ACTGAATGGG
    AAGACACTCA AGACCACCAA GTTCATTGTC CTCTCCCAAG AAGGCTCACT GTGCTCCATC
    TCCATCGAGA AGGCCTTGCC CGAGGACAGA GGTTTGTACA AGTGTGTGGC CAAGAACAGC
    GCCGGCCAGG CTGAGTGCTC CTGCCAAGTC ACCGTGGACG ATGCCCCCAC CGGAGAGAAT
    GCCAAGGCCC CGGAGATGAA AGCCCGGAGG CCAAAGAGCT CTCTTCCTCC TGTGCTAGGA
    ACAGAGAGTG ATGCCACTGT GAAAAAGAAA CCTGCCCCCA AGACACCTCC GAAGGCAGCC
    ATGCCCCCTC AGATCATCCA GTTTCCCGAG GACCAAAAGG TGCGAGCGGG AGAGTCTGTG
    GAGCTGTTTG GTAAAGTGGC CGGTACCCAG CCCATCACCT GTACCTGGAT GAAGTTCCGA
    AAGCAGATCC AGGAGAGTGA GCACATCAAG GTGGAGAACA GTGAGAATGG CAGCAAGCTC
    ACCATCCGGG CCGCGCGCCA GGAGCACTGT GGCTGCTACA CTCTGCTGGT GGAGAACAAG
    CTGGGCAGCA GGCAGGCCCA GGTCAACCTC ACTGTTGTGG ACAAGCCGGA CCCCCCCGCA
    GGCACGCCTT GTGCCTCTGA CATCCGCAGC TCCTCGCTGA CCCTGTCCTG GTATGGCTCC
    TCCTACGATG GGGGCAGCGC CGTACAGTCC TACAGCGTCG AGATCTGGGA CTCAGTGGAC
    AAGACGTGGA AGGAACTAGC CACATGCCGC AGCACCTCTT TTAACGTCCA GGACCTGCTG
    TCTGACCGGG AGTATAAATT CCGTGTGCGT GCAATCAACA TCTACGGAAC CAGTGAGCCG
    AGCCAGGAGT CTGAACTCAC AGCGGTGGGA GAGAAACCTG AGGAGCCCAA GGATGAAGTG
    GAGGTGTCAG ATGACGATGA GAAGGAGCCT GAGGTCGATT ACCGGACCGT CACGGTCAAT
    ACGGAACAAA AAGTGTCTGA CTTCTACGAC ATTGAAGAGA GACTAGGATC TGGGAAATTT
    GGACAGGTCT TCCGACTTGT AGAGAAGAAA ACTGGAAAAA TCTGGGCAGG GAAATTCTTC
    AAGGCGTATT CAGCAAAAGA GAAAGAGAAC ATCCGGCAGG AGATCAGCAT CATGAACTGC
    CTCCACCACC CCAAGCTGGT CCAGTGTGTG GACGCCTTCG AAGAGAAGGC CAACATCGTC
    ATGGTCCTGG AGATCGTGTC CGGGGGCGAG CTGTTCGAGC GCATCATTGA CGAGGACTTT
    GAGCTGACGG AGCGCGAGTG CATCAAGTAC ATGCGGCAGA TCTCGGAGGG GGTGGAGTAC
    ATCCACAAGC AGGGCATCGT CCACCTGGAC CTCAAGCCTG AGAACATCAT GTGTGTCAAC
    AAGACGGGCA CCCGGATCAA GCTCATCGAC TTCGGTCTGG CCCGGCGGCT GGAGAATGCG
    GGGTCTCTGA AGGTCCTCTT TGGCACCCCG GAGTTTGTGG CGCCTGAAGT GATCAACTAT
    GAGCCCATTG GCTATGCCAC GGACATGTGG AGCATCGGGG TCATCTGCTA CATCCTGGTC
    AGTGGACTGT CCCCCTTTAT GGGTGACAAC GATAACGAGA CCTTAGCCAA CGTTACCTCA
    GCCACCTGGG ACTTCGACGA TGAGGCCTTC GATGAGATCT CCGACGACGC CAAGGATTTC
    ATCAGCAATT TACTGAAGAA AGATATGAAA AACCGCTTGG ACTGCACCCA GTGCCTCCAG
    CACCCATGGC TAATGAAAGA TACCAAGAAC ATGGAGGCCA AGAAGCTCTC CAAGGACCGG
    ATGAAAAAGT ACATGGCAAG GAGGAAATGG CAGAAAACGG GCAATGCTGT GAGAGCCATT
    GGAAGACTGT CCTCTATGGC AATGATCTCA GGGCTCAGTG GCAGGAAATC CTCAACAGGG
    TCACCAACCA GCCCGCTGAA TGCAGAAAAA TTAGAATCTG AAGAAGATGT CTCCCAAGCT
    TTCCTTGAGG CCGTCGCTGA GGAAAAGCCC CATGTAAAAC CCTATTTTTC TAAGACCATT
    CGTGATTTAG AAGTTGTGGA GGGAAGCGCT GCTAGATTTG ACTGCAAGAT TGAAGGATAC
    CCAGACCCTG AGGTTGTCTG GTTCAAAGAT GACCAGTCAA TCAGGGAGTC CCGCCACTTC
    CAGATAGACT ACGATGAGGA TGGGAACTGC TCTTTAATCA TTAGCGATGT CTGCGGTGAT
    GATGATGCCA AGTACACCTG CAAGGCTGTC AACAGTCTTG GAGAAGCCAC CTGCACAGCA
    GAGCTCATTG TGGAAACCAT GGAGGAAGGA GAAGGGGAAG GGGAAGAAGA AGAGGAGGAA
    GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020925892.1
    CDS265..6210
    Translation

    Target ORF information:

    RefSeq Version XM_021070233.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa myosin light chain kinase (MYLK), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021070233.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGATCACT CGAGAGTCGG CTCCACGCCC CTGACCGAGG CCCCTGCTTT CATTTTGCCC 
    CCTCGGAACC TTTGCATCAA GGAAGGAGCC ACTGCCAAGT TTGAAGGGAG GGTCCGGGGT
    TACCCAGAGC CCCAGGTGAC ATGGCACAGA AACGGACAGC CCATCACCAG TGGAGGTCGC
    TTCCTGCTGG ACTGTGGCAT CCGGGGGACC TTCAGTCTTG TGATTCATTC TGTCTGTGAG
    GAGGACAAGG GAAAGTATAC CTGTGAAGCC ACCAATGGTA GTGGCGCCCG CCAGGTGACA
    GTGGAGTTGA CGGTAGAAGG GGGCTTTGTG AAGAAGCATG GCCAGCCTGT TGTTTCCAAA
    GCCTTGGGGG ACAGATTTGC AGCCCCTGCA GTCGAGACCC GTCCTAGCAT CTGGGGAGAG
    TGCCCACCAA AGTTTGCCAC CAAGCTGGGC CGAGCGGTGG TCAAAGAAGG ACAGATGGGG
    CGATTCTCCT GCAAGATCAC TGGCCGGCCC CAACCGCAGG TCACCTGGCT CAAGGGAGAT
    GTTCCCCTGC AGCCCAGTGC CCGTGTGACC ATGTCTGAGA AGAATGGGGT GCAAGTGCTG
    GAAATCCACG AAGTCAACCG AGACGACGTG GGGGTGTACA CGTGCATGGT GGTGAATGGG
    TCGGGGAAGG CCTCCATGTC AGCTGAGCTC TCCATCCAAG GTTTGGACAA TACCAATAGG
    TCGCTTGTGA GAGGAACAAA GGCGGCCAGT TCAGACATCA GGACAGAAGC GACCAATGGA
    CTCTCACAGG GGCCAAAGCT GGATGGCCTG GAGGCTGCAG CGGAGAGTAA GAACTGCTCC
    AGCGCCCAGA GGGGTGGCTC CCCGACCTGG GCTTCAGGGA GCCAGCCTCA GCCTCCGAGG
    GAATCCAAGC TGGAGCCGTC AGGGGGCTCA GACAGAAAAG CCCTGAGGAG CCCCATCCTG
    CAGAAGACTT CCAGTGCCAT CACCCTGCAG CCTGCAAAAG TCCAGTTGGA ACCAAGGGCT
    CCAGTCTCAG GCACCCTCTT GCCTTCCAGA GAAGAGAGAG AGCGGCCAGC TGCTCCACCT
    GCCGCCACCC TGCCCTCCAG ACAGTCTGGC ACAGGAGGCC AAGAAGCTGT GAGCAAGGCT
    GCTACCAAGC AGGTTGCCAT GGAGAGCCGA AGGGCTGCCA CAGTCCCCAG ATTCGAGAGC
    AAGCCCCAAA GCCAGGAGGT CAGTGAAGAT CAGCCAGTCA AGTTCAAGTG TGAAGTTTCA
    GGGGTTCCAA AGCCCGAAGT GACCTGGTTC CTGGACGGTG CCCCTGTGAA GAGGCGAGAA
    GGCACCTTTG AGGTTTATGA AGCCGGAGGA TCCCACTACC TCTGCCTGCC AAGAGCCCGG
    GCCAGGGACA GCGGGAACTA CAGCTGCACA GCCACCAACA TCCGAGGCCA GGTGTCCTGT
    ACCTGGACCC TCCTGGTGAA AAGGCTGGCC GTGACAGAGG CGCCCCCGTC CTTCTCCAGT
    GTCCTGAAGG ACTGTGCTGT GGTTGAGGGC CAGGACTTCG TGCTGCAGTG CTCCGTGCAG
    GGGACCCCGC TGCCCCAGAT CACTTGGCTG CTCAATGGGC AGCCCATCCA GTACGCCCGC
    TCCACCTGCG AGGCCGGCGT TGCCAAGCTC CACATCCAGG ATGCCCTCCC GGAGGACGAC
    GGCACCTATA CCTGTCTGGC CGAGAACACC TTGGGGCGGG TGTCCTGCAG CGCCCGGGTC
    ACTGTCCATG AAAAGAAGAG TGATGGGAAG AGTGAGTACC TCCTGCCCGC TGCCCCCAGC
    AAGCCCATGG CACCCATCTT CCTGCAGGGT CTCTCTGATC TCAGCGTCAT GGATGGGAGC
    CAGGTCACCA TGACAGTCCA GGTGTCAGGG AACCCACCCC CTGAGGTCAT CTGGCTTCAC
    GATGGGAATG AGATCCAGGA GTCGGAGGAC TTCCACTTTG AGCAGAGGGG CACTCGGCAC
    AGCCTCTGTA TCCAGGAAGT GTTCCCAGAA GACACAGGCA CATACACCTG TGAAGCCTGG
    AACAGCGCAG GAGAGGTCCG CACCCAGGCC GTGCTCACGG TGCAAGAGCC TCAGGACGGC
    ACCCAGCCTT GGTTCATCAG CAAGCCCCGC TCTGTGACAG CCTGCCTGGG CCAGAGTGTC
    CTCATCTCCT GTGCCATAGC TGGGGACCCC TTCCCTACTG TGCACTGGCT CCGAGACGGC
    AAAGCCCTCT CCAAAGACAC TGGCCACTTC GAGGTGCTGC AGAATGAGGA CGTGTTCACC
    CTGGTTCTCA AGAACGTGCA GCCCTGGCAC GCCGGCCAGT ATGAGATCCT GCTCAAGAAC
    CGGGTTGGCG AGTGCAGCTG CCAGGTGTCC CTGGTGCTAC AGAGCAGCCC TGCCAGAGCC
    CCCCTGCGGG GGAGGGAGCC TGCCAGCTGT GAGGGCCAAG GAGCTGGTGC TGATGGTGGT
    GGTGCTGGCA GAGACGGCTA CGGGAGCCTG AGGCCCGGCT GGCCAGCAAG AGGGCAGGGT
    GAGCCCGAGG ACGAAGACGG CGAGGACGTG CGCGGGGTGC TCAAGAGGCG CGTGGAGACG
    CGGCAGCACA CCGAGGAGGC TATCCGCCAG CAGGAGGTGG AGCAGCTGGA CTTCCGCGAC
    CTCCTGGGCA AGAAGGTGAG CACCAAGACC TTGTCTGAGG AGGACCTGAA GGAGATCCCT
    GCCGAGCAGA TGGATTTCCG CGCCAATCTG CAGCGGCAAG TCAAGCCGAA GACTGTGTCC
    GAGGAAGAGA GGAAGGTGCA TAGCCCCCAG CAGGTGGACT TCCGCTCGGT CCTGGCCAAG
    AAGGGGACGC CCAAGACCCC CGTACCTGAG AAGCTGCCCC CTCCAAAACC TGCCACCCCA
    GATTTTCGCT CGGTGTTGGG CAGTAAGAAA AAATTACCAG CGGAGAATGG TAGCAGCAAT
    GCTGAGCCTT TGAATGTCAA GGCAGCAGAA GGTCCCAAGC CTGTGGGCAA TGCCCAGCCT
    TCAGGCTTTC TCAAACCTAT GGGCAATGCC AAGCTGGCCG AGACCTCCAA ACACTTGAGT
    AATGCCAAGC CAGCCGAGCT CCCCAAACCT GTGGGCAACG CCAAGCCAGC CGAGATCCCC
    AAACCCGTGG GCAACACCAG GCCAGCTGAG ACCCCCAAAA CCGTGGGCAA TGCCAAGCCA
    ACTGAGACCT TCAAACCCAT GGCCAATACC AAGCCAGCTG AGACCCTAAA GCCTGTGGGC
    AATGCCAAGC CAGCTGAGAC CCTAAAGCCT GTGGGCAATG CCAAGCCAGC TGAGACCCTA
    AAGCCCGTGG GCAATGCCAA GCCAGCTGAG ACCCCCAAGC CCTTGGGTAA TACCAAGCCA
    GCCGAGCCCC CTAAACCAGC TGGGAAAGAA GAGCTTAAGA AGGAGGTTAA GAATGATGTG
    AACTACAAGA GGGGCTCTGC AGGGGCCACA GATAATGAAA AAAGCCCGGA GAGCCAAGGG
    TCAGCCCCAA CCTTCAAGGA AAAGCTACGT GATCTCCGTG TGGCAGAGGG TGAGAAGCTG
    CTGCTCCAGT GCCAGGTATC CTCCGACCCC CCGGCCACCA TCACCTGGAC ACTGAATGGG
    AAGACACTCA AGACCACCAA GTTCATTGTC CTCTCCCAAG AAGGCTCACT GTGCTCCATC
    TCCATCGAGA AGGCCTTGCC CGAGGACAGA GGTTTGTACA AGTGTGTGGC CAAGAACAGC
    GCCGGCCAGG CTGAGTGCTC CTGCCAAGTC ACCGTGGACG ATGCCCCCAC CGGAGAGAAT
    GCCAAGGCCC CGGAGATGAA AGCCCGGAGG CCAAAGAGCT CTCTTCCTCC TGTGCTAGGA
    ACAGAGAGTG ATGCCACTGT GAAAAAGAAA CCTGCCCCCA AGACACCTCC GAAGGCAGCC
    ATGCCCCCTC AGATCATCCA GTTTCCCGAG GACCAAAAGG TGCGAGCGGG AGAGTCTGTG
    GAGCTGTTTG GTAAAGTGGC CGGTACCCAG CCCATCACCT GTACCTGGAT GAAGTTCCGA
    AAGCAGATCC AGGAGAGTGA GCACATCAAG GTGGAGAACA GTGAGAATGG CAGCAAGCTC
    ACCATCCGGG CCGCGCGCCA GGAGCACTGT GGCTGCTACA CTCTGCTGGT GGAGAACAAG
    CTGGGCAGCA GGCAGGCCCA GGTCAACCTC ACTGTTGTGG ACAAGCCGGA CCCCCCCGCA
    GGCACGCCTT GTGCCTCTGA CATCCGCAGC TCCTCGCTGA CCCTGTCCTG GTATGGCTCC
    TCCTACGATG GGGGCAGCGC CGTACAGTCC TACAGCGTCG AGATCTGGGA CTCAGTGGAC
    AAGACGTGGA AGGAACTAGC CACATGCCGC AGCACCTCTT TTAACGTCCA GGACCTGCTG
    TCTGACCGGG AGTATAAATT CCGTGTGCGT GCAATCAACA TCTACGGAAC CAGTGAGCCG
    AGCCAGGAGT CTGAACTCAC AGCGGTGGGA GAGAAACCTG AGGAGCCCAA GGATGAAGTG
    GAGGTGTCAG ATGACGATGA GAAGGAGCCT GAGGTCGATT ACCGGACCGT CACGGTCAAT
    ACGGAACAAA AAGTGTCTGA CTTCTACGAC ATTGAAGAGA GACTAGGATC TGGGAAATTT
    GGACAGGTCT TCCGACTTGT AGAGAAGAAA ACTGGAAAAA TCTGGGCAGG GAAATTCTTC
    AAGGCGTATT CAGCAAAAGA GAAAGAGAAC ATCCGGCAGG AGATCAGCAT CATGAACTGC
    CTCCACCACC CCAAGCTGGT CCAGTGTGTG GACGCCTTCG AAGAGAAGGC CAACATCGTC
    ATGGTCCTGG AGATCGTGTC CGGGGGCGAG CTGTTCGAGC GCATCATTGA CGAGGACTTT
    GAGCTGACGG AGCGCGAGTG CATCAAGTAC ATGCGGCAGA TCTCGGAGGG GGTGGAGTAC
    ATCCACAAGC AGGGCATCGT CCACCTGGAC CTCAAGCCTG AGAACATCAT GTGTGTCAAC
    AAGACGGGCA CCCGGATCAA GCTCATCGAC TTCGGTCTGG CCCGGCGGCT GGAGAATGCG
    GGGTCTCTGA AGGTCCTCTT TGGCACCCCG GAGTTTGTGG CGCCTGAAGT GATCAACTAT
    GAGCCCATTG GCTATGCCAC GGACATGTGG AGCATCGGGG TCATCTGCTA CATCCTGGTC
    AGTGGACTGT CCCCCTTTAT GGGTGACAAC GATAACGAGA CCTTAGCCAA CGTTACCTCA
    GCCACCTGGG ACTTCGACGA TGAGGCCTTC GATGAGATCT CCGACGACGC CAAGGATTTC
    ATCAGCAATT TACTGAAGAA AGATATGAAA AACCGCTTGG ACTGCACCCA GTGCCTCCAG
    CACCCATGGC TAATGAAAGA TACCAAGAAC ATGGAGGCCA AGAAGCTCTC CAAGGACCGG
    ATGAAAAAGT ACATGGCAAG GAGGAAATGG CAGAAAACGG GCAATGCTGT GAGAGCCATT
    GGAAGACTGT CCTCTATGGC AATGATCTCA GGGCTCAGTG GCAGGAAATC CTCAACAGGG
    TCACCAACCA GCCCGCTGAA TGCAGAAAAA TTAGAATCTG AAGAAGATGT CTCCCAAGCT
    TTCCTTGAGG CCGTCGCTGA GGAAAAGCCC CATGTAAAAC CCTATTTTTC TAAGACCATT
    CGTGATTTAG AAGTTGTGGA GGGAAGCGCT GCTAGATTTG ACTGCAAGAT TGAAGGATAC
    CCAGACCCTG AGGTTGTCTG GTTCAAAGAT GACCAGTCAA TCAGGGAGTC CCGCCACTTC
    CAGATAGACT ACGATGAGGA TGGGAACTGC TCTTTAATCA TTAGCGATGT CTGCGGTGAT
    GATGATGCCA AGTACACCTG CAAGGCTGTC AACAGTCTTG GAGAAGCCAC CTGCACAGCA
    GAGCTCATTG TGGAAACCAT GGAGGAAGGA GAAGGGGAAG GGGAAGAAGA AGAGGAGGAA
    GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe236096
    Clone ID Related Accession (Same CDS sequence) XM_021070237.1 , XM_021070236.1
    Accession Version XM_021070237.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5811bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product myosin light chain kinase, smooth muscle isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010455.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGATCACT CGAGAGTCGG CTCCACGCCC CTGACCGAGG CCCCTGCTTT CATTTTGCCC 
    CCTCGGAACC TTTGCATCAA GGAAGGAGCC ACTGCCAAGT TTGAAGGGAG GGTCCGGGGT
    TACCCAGAGC CCCAGGTGAC ATGGCACAGA AACGGACAGC CCATCACCAG TGGAGGTCGC
    TTCCTGCTGG ACTGTGGCAT CCGGGGGACC TTCAGTCTTG TGATTCATTC TGTCTGTGAG
    GAGGACAAGG GAAAGTATAC CTGTGAAGCC ACCAATGGTA GTGGCGCCCG CCAGGTGACA
    GTGGAGTTGA CGGTAGAAGG GGGCTTTGTG AAGAAGCATG GCCAGCCTGT TGTTTCCAAA
    GCCTTGGGGG ACAGATTTGC AGCCCCTGCA GTCGAGACCC GTCCTAGCAT CTGGGGAGAG
    TGCCCACCAA AGTTTGCCAC CAAGCTGGGC CGAGCGGTGG TCAAAGAAGG ACAGATGGGG
    CGATTCTCCT GCAAGATCAC TGGCCGGCCC CAACCGCAGG TCACCTGGCT CAAGGGAGAT
    GTTCCCCTGC AGCCCAGTGC CCGTGTGACC ATGTCTGAGA AGAATGGGGT GCAAGTGCTG
    GAAATCCACG AAGTCAACCG AGACGACGTG GGGGTGTACA CGTGCATGGT GGTGAATGGG
    TCGGGGAAGG CCTCCATGTC AGCTGAGCTC TCCATCCAAG GTTTGGACAA TACCAATAGG
    TCGCTTGTGA GAGGAACAAA GGCGGCCAGT TCAGACATCA GGACAGAAGC GACCAATGGA
    CTCTCACAGG GGCCAAAGCT GGATGGCCTG GAGGCTGCAG CGGAGAGTAA GAACTGCTCC
    AGCGCCCAGA GGGGTGGCTC CCCGACCTGG GCTTCAGGGA GCCAGCCTCA GCCTCCGAGG
    GAATCCAAGC TGGAGCCGTC AGGGGGCTCA GACAGAAAAG CCCTGAGGAG CCCCATCCTG
    CAGAAGACTT CCAGTGCCAT CACCCTGCAG CCTGCAAAAG TCCAGTTGGA ACCAAGGGCT
    CCAGTCTCAG GCACCCTCTT GCCTTCCAGA GAAGAGAGAG AGCGGCCAGC TGCTCCACCT
    GCCGCCACCC TGCCCTCCAG ACAGTCTGGC ACAGGAGGCC AAGAAGCTGT GAGCAAGGCT
    GCTACCAAGC AGGTTGCCAT GGAGAGCCGA AGGGCTGCCA CAGTCCCCAG ATTCGAGAGC
    AAGCCCCAAA GCCAGGAGGT CAGTGAAGAT CAGCCAGTCA AGTTCAAGTG TGAAGTTTCA
    GGGGTTCCAA AGCCCGAAGT GACCTGGTTC CTGGACGGTG CCCCTGTGAA GAGGCGAGAA
    GGCACCTTTG AGGTTTATGA AGCCGGAGGA TCCCACTACC TCTGCCTGCC AAGAGCCCGG
    GCCAGGGACA GCGGGAACTA CAGCTGCACA GCCACCAACA TCCGAGGCCA GGTGTCCTGT
    ACCTGGACCC TCCTGGTGAA AAGGCAGCCC ATCCAGTACG CCCGCTCCAC CTGCGAGGCC
    GGCGTTGCCA AGCTCCACAT CCAGGATGCC CTCCCGGAGG ACGACGGCAC CTATACCTGT
    CTGGCCGAGA ACACCTTGGG GCGGGTGTCC TGCAGCGCCC GGGTCACTGT CCATGAAAAG
    AAGAGTGATG GGAAGAGTGA GTACCTCCTG CCCGCTGCCC CCAGCAAGCC CATGGCACCC
    ATCTTCCTGC AGGGTCTCTC TGATCTCAGC GTCATGGATG GGAGCCAGGT CACCATGACA
    GTCCAGGTGT CAGGGAACCC ACCCCCTGAG GTCATCTGGC TTCACGATGG GAATGAGATC
    CAGGAGTCGG AGGACTTCCA CTTTGAGCAG AGGGGCACTC GGCACAGCCT CTGTATCCAG
    GAAGTGTTCC CAGAAGACAC AGGCACATAC ACCTGTGAAG CCTGGAACAG CGCAGGAGAG
    GTCCGCACCC AGGCCGTGCT CACGGTGCAA GAGCCTCAGG ACGGCACCCA GCCTTGGTTC
    ATCAGCAAGC CCCGCTCTGT GACAGCCTGC CTGGGCCAGA GTGTCCTCAT CTCCTGTGCC
    ATAGCTGGGG ACCCCTTCCC TACTGTGCAC TGGCTCCGAG ACGGCAAAGC CCTCTCCAAA
    GACACTGGCC ACTTCGAGGT GCTGCAGAAT GAGGACGTGT TCACCCTGGT TCTCAAGAAC
    GTGCAGCCCT GGCACGCCGG CCAGTATGAG ATCCTGCTCA AGAACCGGGT TGGCGAGTGC
    AGCTGCCAGG TGTCCCTGGT GCTACAGAGC AGCCCTGCCA GAGCCCCCCT GCGGGGGAGG
    GAGCCTGCCA GCTGTGAGGG CCAAGGAGCT GGTGCTGATG GTGGTGGTGC TGGCAGAGAC
    GGCTACGGGA GCCTGAGGCC CGGCTGGCCA GCAAGAGGGC AGGGTGAGCC CGAGGACGAA
    GACGGCGAGG ACGTGCGCGG GGTGCTCAAG AGGCGCGTGG AGACGCGGCA GCACACCGAG
    GAGGCTATCC GCCAGCAGGA GGTGGAGCAG CTGGACTTCC GCGACCTCCT GGGCAAGAAG
    GTGAGCACCA AGACCTTGTC TGAGGAGGAC CTGAAGGAGA TCCCTGCCGA GCAGATGGAT
    TTCCGCGCCA ATCTGCAGCG GCAAGTCAAG CCGAAGACTG TGTCCGAGGA AGAGAGGAAG
    GTGCATAGCC CCCAGCAGGT GGACTTCCGC TCGGTCCTGG CCAAGAAGGG GACGCCCAAG
    ACCCCCGTAC CTGAGAAGCT GCCCCCTCCA AAACCTGCCA CCCCAGATTT TCGCTCGGTG
    TTGGGCAGTA AGAAAAAATT ACCAGCGGAG AATGGTAGCA GCAATGCTGA GCCTTTGAAT
    GTCAAGGCAG CAGAAGGTCC CAAGCCTGTG GGCAATGCCC AGCCTTCAGG CTTTCTCAAA
    CCTATGGGCA ATGCCAAGCT GGCCGAGACC TCCAAACACT TGAGTAATGC CAAGCCAGCC
    GAGCTCCCCA AACCTGTGGG CAACGCCAAG CCAGCCGAGA TCCCCAAACC CGTGGGCAAC
    ACCAGGCCAG CTGAGACCCC CAAAACCGTG GGCAATGCCA AGCCAACTGA GACCTTCAAA
    CCCATGGCCA ATACCAAGCC AGCTGAGACC CTAAAGCCTG TGGGCAATGC CAAGCCAGCT
    GAGACCCTAA AGCCTGTGGG CAATGCCAAG CCAGCTGAGA CCCTAAAGCC CGTGGGCAAT
    GCCAAGCCAG CTGAGACCCC CAAGCCCTTG GGTAATACCA AGCCAGCCGA GCCCCCTAAA
    CCAGCTGGGA AAGAAGAGCT TAAGAAGGAG GTTAAGAATG ATGTGAACTA CAAGAGGGGC
    TCTGCAGGGG CCACAGATAA TGAAAAAAGC CCGGAGAGCC AAGGGTCAGC CCCAACCTTC
    AAGGAAAAGC TACGTGATCT CCGTGTGGCA GAGGGTGAGA AGCTGCTGCT CCAGTGCCAG
    GTATCCTCCG ACCCCCCGGC CACCATCACC TGGACACTGA ATGGGAAGAC ACTCAAGACC
    ACCAAGTTCA TTGTCCTCTC CCAAGAAGGC TCACTGTGCT CCATCTCCAT CGAGAAGGCC
    TTGCCCGAGG ACAGAGGTTT GTACAAGTGT GTGGCCAAGA ACAGCGCCGG CCAGGCTGAG
    TGCTCCTGCC AAGTCACCGT GGACGATGCC CCCACCGGAG AGAATGCCAA GGCCCCGGAG
    ATGAAAGCCC GGAGGCCAAA GAGCTCTCTT CCTCCTGTGC TAGGAACAGA GAGTGATGCC
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCGAAGG CAGCCATGCC CCCTCAGATC
    ATCCAGTTTC CCGAGGACCA AAAGGTGCGA GCGGGAGAGT CTGTGGAGCT GTTTGGTAAA
    GTGGCCGGTA CCCAGCCCAT CACCTGTACC TGGATGAAGT TCCGAAAGCA GATCCAGGAG
    AGTGAGCACA TCAAGGTGGA GAACAGTGAG AATGGCAGCA AGCTCACCAT CCGGGCCGCG
    CGCCAGGAGC ACTGTGGCTG CTACACTCTG CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG
    GCCCAGGTCA ACCTCACTGT TGTGGACAAG CCGGACCCCC CCGCAGGCAC GCCTTGTGCC
    TCTGACATCC GCAGCTCCTC GCTGACCCTG TCCTGGTATG GCTCCTCCTA CGATGGGGGC
    AGCGCCGTAC AGTCCTACAG CGTCGAGATC TGGGACTCAG TGGACAAGAC GTGGAAGGAA
    CTAGCCACAT GCCGCAGCAC CTCTTTTAAC GTCCAGGACC TGCTGTCTGA CCGGGAGTAT
    AAATTCCGTG TGCGTGCAAT CAACATCTAC GGAACCAGTG AGCCGAGCCA GGAGTCTGAA
    CTCACAGCGG TGGGAGAGAA ACCTGAGGAG CCCAAGGATG AAGTGGAGGT GTCAGATGAC
    GATGAGAAGG AGCCTGAGGT CGATTACCGG ACCGTCACGG TCAATACGGA ACAAAAAGTG
    TCTGACTTCT ACGACATTGA AGAGAGACTA GGATCTGGGA AATTTGGACA GGTCTTCCGA
    CTTGTAGAGA AGAAAACTGG AAAAATCTGG GCAGGGAAAT TCTTCAAGGC GTATTCAGCA
    AAAGAGAAAG AGAACATCCG GCAGGAGATC AGCATCATGA ACTGCCTCCA CCACCCCAAG
    CTGGTCCAGT GTGTGGACGC CTTCGAAGAG AAGGCCAACA TCGTCATGGT CCTGGAGATC
    GTGTCCGGGG GCGAGCTGTT CGAGCGCATC ATTGACGAGG ACTTTGAGCT GACGGAGCGC
    GAGTGCATCA AGTACATGCG GCAGATCTCG GAGGGGGTGG AGTACATCCA CAAGCAGGGC
    ATCGTCCACC TGGACCTCAA GCCTGAGAAC ATCATGTGTG TCAACAAGAC GGGCACCCGG
    ATCAAGCTCA TCGACTTCGG TCTGGCCCGG CGGCTGGAGA ATGCGGGGTC TCTGAAGGTC
    CTCTTTGGCA CCCCGGAGTT TGTGGCGCCT GAAGTGATCA ACTATGAGCC CATTGGCTAT
    GCCACGGACA TGTGGAGCAT CGGGGTCATC TGCTACATCC TGGTCAGTGG ACTGTCCCCC
    TTTATGGGTG ACAACGATAA CGAGACCTTA GCCAACGTTA CCTCAGCCAC CTGGGACTTC
    GACGATGAGG CCTTCGATGA GATCTCCGAC GACGCCAAGG ATTTCATCAG CAATTTACTG
    AAGAAAGATA TGAAAAACCG CTTGGACTGC ACCCAGTGCC TCCAGCACCC ATGGCTAATG
    AAAGATACCA AGAACATGGA GGCCAAGAAG CTCTCCAAGG ACCGGATGAA AAAGTACATG
    GCAAGGAGGA AATGGCAGAA AACGGGCAAT GCTGTGAGAG CCATTGGAAG ACTGTCCTCT
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG
    CTGAATGCAG AAAAATTAGA ATCTGAAGAA GATGTCTCCC AAGCTTTCCT TGAGGCCGTC
    GCTGAGGAAA AGCCCCATGT AAAACCCTAT TTTTCTAAGA CCATTCGTGA TTTAGAAGTT
    GTGGAGGGAA GCGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCTGAGGTT
    GTCTGGTTCA AAGATGACCA GTCAATCAGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT
    GAGGATGGGA ACTGCTCTTT AATCATTAGC GATGTCTGCG GTGATGATGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAACAG TCTTGGAGAA GCCACCTGCA CAGCAGAGCT CATTGTGGAA
    ACCATGGAGG AAGGAGAAGG GGAAGGGGAA GAAGAAGAGG AGGAAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020925896.1
    CDS272..6082
    Translation

    Target ORF information:

    RefSeq Version XM_021070237.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa myosin light chain kinase (MYLK), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021070237.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGATCACT CGAGAGTCGG CTCCACGCCC CTGACCGAGG CCCCTGCTTT CATTTTGCCC 
    CCTCGGAACC TTTGCATCAA GGAAGGAGCC ACTGCCAAGT TTGAAGGGAG GGTCCGGGGT
    TACCCAGAGC CCCAGGTGAC ATGGCACAGA AACGGACAGC CCATCACCAG TGGAGGTCGC
    TTCCTGCTGG ACTGTGGCAT CCGGGGGACC TTCAGTCTTG TGATTCATTC TGTCTGTGAG
    GAGGACAAGG GAAAGTATAC CTGTGAAGCC ACCAATGGTA GTGGCGCCCG CCAGGTGACA
    GTGGAGTTGA CGGTAGAAGG GGGCTTTGTG AAGAAGCATG GCCAGCCTGT TGTTTCCAAA
    GCCTTGGGGG ACAGATTTGC AGCCCCTGCA GTCGAGACCC GTCCTAGCAT CTGGGGAGAG
    TGCCCACCAA AGTTTGCCAC CAAGCTGGGC CGAGCGGTGG TCAAAGAAGG ACAGATGGGG
    CGATTCTCCT GCAAGATCAC TGGCCGGCCC CAACCGCAGG TCACCTGGCT CAAGGGAGAT
    GTTCCCCTGC AGCCCAGTGC CCGTGTGACC ATGTCTGAGA AGAATGGGGT GCAAGTGCTG
    GAAATCCACG AAGTCAACCG AGACGACGTG GGGGTGTACA CGTGCATGGT GGTGAATGGG
    TCGGGGAAGG CCTCCATGTC AGCTGAGCTC TCCATCCAAG GTTTGGACAA TACCAATAGG
    TCGCTTGTGA GAGGAACAAA GGCGGCCAGT TCAGACATCA GGACAGAAGC GACCAATGGA
    CTCTCACAGG GGCCAAAGCT GGATGGCCTG GAGGCTGCAG CGGAGAGTAA GAACTGCTCC
    AGCGCCCAGA GGGGTGGCTC CCCGACCTGG GCTTCAGGGA GCCAGCCTCA GCCTCCGAGG
    GAATCCAAGC TGGAGCCGTC AGGGGGCTCA GACAGAAAAG CCCTGAGGAG CCCCATCCTG
    CAGAAGACTT CCAGTGCCAT CACCCTGCAG CCTGCAAAAG TCCAGTTGGA ACCAAGGGCT
    CCAGTCTCAG GCACCCTCTT GCCTTCCAGA GAAGAGAGAG AGCGGCCAGC TGCTCCACCT
    GCCGCCACCC TGCCCTCCAG ACAGTCTGGC ACAGGAGGCC AAGAAGCTGT GAGCAAGGCT
    GCTACCAAGC AGGTTGCCAT GGAGAGCCGA AGGGCTGCCA CAGTCCCCAG ATTCGAGAGC
    AAGCCCCAAA GCCAGGAGGT CAGTGAAGAT CAGCCAGTCA AGTTCAAGTG TGAAGTTTCA
    GGGGTTCCAA AGCCCGAAGT GACCTGGTTC CTGGACGGTG CCCCTGTGAA GAGGCGAGAA
    GGCACCTTTG AGGTTTATGA AGCCGGAGGA TCCCACTACC TCTGCCTGCC AAGAGCCCGG
    GCCAGGGACA GCGGGAACTA CAGCTGCACA GCCACCAACA TCCGAGGCCA GGTGTCCTGT
    ACCTGGACCC TCCTGGTGAA AAGGCAGCCC ATCCAGTACG CCCGCTCCAC CTGCGAGGCC
    GGCGTTGCCA AGCTCCACAT CCAGGATGCC CTCCCGGAGG ACGACGGCAC CTATACCTGT
    CTGGCCGAGA ACACCTTGGG GCGGGTGTCC TGCAGCGCCC GGGTCACTGT CCATGAAAAG
    AAGAGTGATG GGAAGAGTGA GTACCTCCTG CCCGCTGCCC CCAGCAAGCC CATGGCACCC
    ATCTTCCTGC AGGGTCTCTC TGATCTCAGC GTCATGGATG GGAGCCAGGT CACCATGACA
    GTCCAGGTGT CAGGGAACCC ACCCCCTGAG GTCATCTGGC TTCACGATGG GAATGAGATC
    CAGGAGTCGG AGGACTTCCA CTTTGAGCAG AGGGGCACTC GGCACAGCCT CTGTATCCAG
    GAAGTGTTCC CAGAAGACAC AGGCACATAC ACCTGTGAAG CCTGGAACAG CGCAGGAGAG
    GTCCGCACCC AGGCCGTGCT CACGGTGCAA GAGCCTCAGG ACGGCACCCA GCCTTGGTTC
    ATCAGCAAGC CCCGCTCTGT GACAGCCTGC CTGGGCCAGA GTGTCCTCAT CTCCTGTGCC
    ATAGCTGGGG ACCCCTTCCC TACTGTGCAC TGGCTCCGAG ACGGCAAAGC CCTCTCCAAA
    GACACTGGCC ACTTCGAGGT GCTGCAGAAT GAGGACGTGT TCACCCTGGT TCTCAAGAAC
    GTGCAGCCCT GGCACGCCGG CCAGTATGAG ATCCTGCTCA AGAACCGGGT TGGCGAGTGC
    AGCTGCCAGG TGTCCCTGGT GCTACAGAGC AGCCCTGCCA GAGCCCCCCT GCGGGGGAGG
    GAGCCTGCCA GCTGTGAGGG CCAAGGAGCT GGTGCTGATG GTGGTGGTGC TGGCAGAGAC
    GGCTACGGGA GCCTGAGGCC CGGCTGGCCA GCAAGAGGGC AGGGTGAGCC CGAGGACGAA
    GACGGCGAGG ACGTGCGCGG GGTGCTCAAG AGGCGCGTGG AGACGCGGCA GCACACCGAG
    GAGGCTATCC GCCAGCAGGA GGTGGAGCAG CTGGACTTCC GCGACCTCCT GGGCAAGAAG
    GTGAGCACCA AGACCTTGTC TGAGGAGGAC CTGAAGGAGA TCCCTGCCGA GCAGATGGAT
    TTCCGCGCCA ATCTGCAGCG GCAAGTCAAG CCGAAGACTG TGTCCGAGGA AGAGAGGAAG
    GTGCATAGCC CCCAGCAGGT GGACTTCCGC TCGGTCCTGG CCAAGAAGGG GACGCCCAAG
    ACCCCCGTAC CTGAGAAGCT GCCCCCTCCA AAACCTGCCA CCCCAGATTT TCGCTCGGTG
    TTGGGCAGTA AGAAAAAATT ACCAGCGGAG AATGGTAGCA GCAATGCTGA GCCTTTGAAT
    GTCAAGGCAG CAGAAGGTCC CAAGCCTGTG GGCAATGCCC AGCCTTCAGG CTTTCTCAAA
    CCTATGGGCA ATGCCAAGCT GGCCGAGACC TCCAAACACT TGAGTAATGC CAAGCCAGCC
    GAGCTCCCCA AACCTGTGGG CAACGCCAAG CCAGCCGAGA TCCCCAAACC CGTGGGCAAC
    ACCAGGCCAG CTGAGACCCC CAAAACCGTG GGCAATGCCA AGCCAACTGA GACCTTCAAA
    CCCATGGCCA ATACCAAGCC AGCTGAGACC CTAAAGCCTG TGGGCAATGC CAAGCCAGCT
    GAGACCCTAA AGCCTGTGGG CAATGCCAAG CCAGCTGAGA CCCTAAAGCC CGTGGGCAAT
    GCCAAGCCAG CTGAGACCCC CAAGCCCTTG GGTAATACCA AGCCAGCCGA GCCCCCTAAA
    CCAGCTGGGA AAGAAGAGCT TAAGAAGGAG GTTAAGAATG ATGTGAACTA CAAGAGGGGC
    TCTGCAGGGG CCACAGATAA TGAAAAAAGC CCGGAGAGCC AAGGGTCAGC CCCAACCTTC
    AAGGAAAAGC TACGTGATCT CCGTGTGGCA GAGGGTGAGA AGCTGCTGCT CCAGTGCCAG
    GTATCCTCCG ACCCCCCGGC CACCATCACC TGGACACTGA ATGGGAAGAC ACTCAAGACC
    ACCAAGTTCA TTGTCCTCTC CCAAGAAGGC TCACTGTGCT CCATCTCCAT CGAGAAGGCC
    TTGCCCGAGG ACAGAGGTTT GTACAAGTGT GTGGCCAAGA ACAGCGCCGG CCAGGCTGAG
    TGCTCCTGCC AAGTCACCGT GGACGATGCC CCCACCGGAG AGAATGCCAA GGCCCCGGAG
    ATGAAAGCCC GGAGGCCAAA GAGCTCTCTT CCTCCTGTGC TAGGAACAGA GAGTGATGCC
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCGAAGG CAGCCATGCC CCCTCAGATC
    ATCCAGTTTC CCGAGGACCA AAAGGTGCGA GCGGGAGAGT CTGTGGAGCT GTTTGGTAAA
    GTGGCCGGTA CCCAGCCCAT CACCTGTACC TGGATGAAGT TCCGAAAGCA GATCCAGGAG
    AGTGAGCACA TCAAGGTGGA GAACAGTGAG AATGGCAGCA AGCTCACCAT CCGGGCCGCG
    CGCCAGGAGC ACTGTGGCTG CTACACTCTG CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG
    GCCCAGGTCA ACCTCACTGT TGTGGACAAG CCGGACCCCC CCGCAGGCAC GCCTTGTGCC
    TCTGACATCC GCAGCTCCTC GCTGACCCTG TCCTGGTATG GCTCCTCCTA CGATGGGGGC
    AGCGCCGTAC AGTCCTACAG CGTCGAGATC TGGGACTCAG TGGACAAGAC GTGGAAGGAA
    CTAGCCACAT GCCGCAGCAC CTCTTTTAAC GTCCAGGACC TGCTGTCTGA CCGGGAGTAT
    AAATTCCGTG TGCGTGCAAT CAACATCTAC GGAACCAGTG AGCCGAGCCA GGAGTCTGAA
    CTCACAGCGG TGGGAGAGAA ACCTGAGGAG CCCAAGGATG AAGTGGAGGT GTCAGATGAC
    GATGAGAAGG AGCCTGAGGT CGATTACCGG ACCGTCACGG TCAATACGGA ACAAAAAGTG
    TCTGACTTCT ACGACATTGA AGAGAGACTA GGATCTGGGA AATTTGGACA GGTCTTCCGA
    CTTGTAGAGA AGAAAACTGG AAAAATCTGG GCAGGGAAAT TCTTCAAGGC GTATTCAGCA
    AAAGAGAAAG AGAACATCCG GCAGGAGATC AGCATCATGA ACTGCCTCCA CCACCCCAAG
    CTGGTCCAGT GTGTGGACGC CTTCGAAGAG AAGGCCAACA TCGTCATGGT CCTGGAGATC
    GTGTCCGGGG GCGAGCTGTT CGAGCGCATC ATTGACGAGG ACTTTGAGCT GACGGAGCGC
    GAGTGCATCA AGTACATGCG GCAGATCTCG GAGGGGGTGG AGTACATCCA CAAGCAGGGC
    ATCGTCCACC TGGACCTCAA GCCTGAGAAC ATCATGTGTG TCAACAAGAC GGGCACCCGG
    ATCAAGCTCA TCGACTTCGG TCTGGCCCGG CGGCTGGAGA ATGCGGGGTC TCTGAAGGTC
    CTCTTTGGCA CCCCGGAGTT TGTGGCGCCT GAAGTGATCA ACTATGAGCC CATTGGCTAT
    GCCACGGACA TGTGGAGCAT CGGGGTCATC TGCTACATCC TGGTCAGTGG ACTGTCCCCC
    TTTATGGGTG ACAACGATAA CGAGACCTTA GCCAACGTTA CCTCAGCCAC CTGGGACTTC
    GACGATGAGG CCTTCGATGA GATCTCCGAC GACGCCAAGG ATTTCATCAG CAATTTACTG
    AAGAAAGATA TGAAAAACCG CTTGGACTGC ACCCAGTGCC TCCAGCACCC ATGGCTAATG
    AAAGATACCA AGAACATGGA GGCCAAGAAG CTCTCCAAGG ACCGGATGAA AAAGTACATG
    GCAAGGAGGA AATGGCAGAA AACGGGCAAT GCTGTGAGAG CCATTGGAAG ACTGTCCTCT
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG
    CTGAATGCAG AAAAATTAGA ATCTGAAGAA GATGTCTCCC AAGCTTTCCT TGAGGCCGTC
    GCTGAGGAAA AGCCCCATGT AAAACCCTAT TTTTCTAAGA CCATTCGTGA TTTAGAAGTT
    GTGGAGGGAA GCGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCTGAGGTT
    GTCTGGTTCA AAGATGACCA GTCAATCAGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT
    GAGGATGGGA ACTGCTCTTT AATCATTAGC GATGTCTGCG GTGATGATGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAACAG TCTTGGAGAA GCCACCTGCA CAGCAGAGCT CATTGTGGAA
    ACCATGGAGG AAGGAGAAGG GGAAGGGGAA GAAGAAGAGG AGGAAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe236096
    Clone ID Related Accession (Same CDS sequence) XM_021070237.1 , XM_021070236.1
    Accession Version XM_021070236.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5811bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product myosin light chain kinase, smooth muscle isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010455.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGATCACT CGAGAGTCGG CTCCACGCCC CTGACCGAGG CCCCTGCTTT CATTTTGCCC 
    CCTCGGAACC TTTGCATCAA GGAAGGAGCC ACTGCCAAGT TTGAAGGGAG GGTCCGGGGT
    TACCCAGAGC CCCAGGTGAC ATGGCACAGA AACGGACAGC CCATCACCAG TGGAGGTCGC
    TTCCTGCTGG ACTGTGGCAT CCGGGGGACC TTCAGTCTTG TGATTCATTC TGTCTGTGAG
    GAGGACAAGG GAAAGTATAC CTGTGAAGCC ACCAATGGTA GTGGCGCCCG CCAGGTGACA
    GTGGAGTTGA CGGTAGAAGG GGGCTTTGTG AAGAAGCATG GCCAGCCTGT TGTTTCCAAA
    GCCTTGGGGG ACAGATTTGC AGCCCCTGCA GTCGAGACCC GTCCTAGCAT CTGGGGAGAG
    TGCCCACCAA AGTTTGCCAC CAAGCTGGGC CGAGCGGTGG TCAAAGAAGG ACAGATGGGG
    CGATTCTCCT GCAAGATCAC TGGCCGGCCC CAACCGCAGG TCACCTGGCT CAAGGGAGAT
    GTTCCCCTGC AGCCCAGTGC CCGTGTGACC ATGTCTGAGA AGAATGGGGT GCAAGTGCTG
    GAAATCCACG AAGTCAACCG AGACGACGTG GGGGTGTACA CGTGCATGGT GGTGAATGGG
    TCGGGGAAGG CCTCCATGTC AGCTGAGCTC TCCATCCAAG GTTTGGACAA TACCAATAGG
    TCGCTTGTGA GAGGAACAAA GGCGGCCAGT TCAGACATCA GGACAGAAGC GACCAATGGA
    CTCTCACAGG GGCCAAAGCT GGATGGCCTG GAGGCTGCAG CGGAGAGTAA GAACTGCTCC
    AGCGCCCAGA GGGGTGGCTC CCCGACCTGG GCTTCAGGGA GCCAGCCTCA GCCTCCGAGG
    GAATCCAAGC TGGAGCCGTC AGGGGGCTCA GACAGAAAAG CCCTGAGGAG CCCCATCCTG
    CAGAAGACTT CCAGTGCCAT CACCCTGCAG CCTGCAAAAG TCCAGTTGGA ACCAAGGGCT
    CCAGTCTCAG GCACCCTCTT GCCTTCCAGA GAAGAGAGAG AGCGGCCAGC TGCTCCACCT
    GCCGCCACCC TGCCCTCCAG ACAGTCTGGC ACAGGAGGCC AAGAAGCTGT GAGCAAGGCT
    GCTACCAAGC AGGTTGCCAT GGAGAGCCGA AGGGCTGCCA CAGTCCCCAG ATTCGAGAGC
    AAGCCCCAAA GCCAGGAGGT CAGTGAAGAT CAGCCAGTCA AGTTCAAGTG TGAAGTTTCA
    GGGGTTCCAA AGCCCGAAGT GACCTGGTTC CTGGACGGTG CCCCTGTGAA GAGGCGAGAA
    GGCACCTTTG AGGTTTATGA AGCCGGAGGA TCCCACTACC TCTGCCTGCC AAGAGCCCGG
    GCCAGGGACA GCGGGAACTA CAGCTGCACA GCCACCAACA TCCGAGGCCA GGTGTCCTGT
    ACCTGGACCC TCCTGGTGAA AAGGCAGCCC ATCCAGTACG CCCGCTCCAC CTGCGAGGCC
    GGCGTTGCCA AGCTCCACAT CCAGGATGCC CTCCCGGAGG ACGACGGCAC CTATACCTGT
    CTGGCCGAGA ACACCTTGGG GCGGGTGTCC TGCAGCGCCC GGGTCACTGT CCATGAAAAG
    AAGAGTGATG GGAAGAGTGA GTACCTCCTG CCCGCTGCCC CCAGCAAGCC CATGGCACCC
    ATCTTCCTGC AGGGTCTCTC TGATCTCAGC GTCATGGATG GGAGCCAGGT CACCATGACA
    GTCCAGGTGT CAGGGAACCC ACCCCCTGAG GTCATCTGGC TTCACGATGG GAATGAGATC
    CAGGAGTCGG AGGACTTCCA CTTTGAGCAG AGGGGCACTC GGCACAGCCT CTGTATCCAG
    GAAGTGTTCC CAGAAGACAC AGGCACATAC ACCTGTGAAG CCTGGAACAG CGCAGGAGAG
    GTCCGCACCC AGGCCGTGCT CACGGTGCAA GAGCCTCAGG ACGGCACCCA GCCTTGGTTC
    ATCAGCAAGC CCCGCTCTGT GACAGCCTGC CTGGGCCAGA GTGTCCTCAT CTCCTGTGCC
    ATAGCTGGGG ACCCCTTCCC TACTGTGCAC TGGCTCCGAG ACGGCAAAGC CCTCTCCAAA
    GACACTGGCC ACTTCGAGGT GCTGCAGAAT GAGGACGTGT TCACCCTGGT TCTCAAGAAC
    GTGCAGCCCT GGCACGCCGG CCAGTATGAG ATCCTGCTCA AGAACCGGGT TGGCGAGTGC
    AGCTGCCAGG TGTCCCTGGT GCTACAGAGC AGCCCTGCCA GAGCCCCCCT GCGGGGGAGG
    GAGCCTGCCA GCTGTGAGGG CCAAGGAGCT GGTGCTGATG GTGGTGGTGC TGGCAGAGAC
    GGCTACGGGA GCCTGAGGCC CGGCTGGCCA GCAAGAGGGC AGGGTGAGCC CGAGGACGAA
    GACGGCGAGG ACGTGCGCGG GGTGCTCAAG AGGCGCGTGG AGACGCGGCA GCACACCGAG
    GAGGCTATCC GCCAGCAGGA GGTGGAGCAG CTGGACTTCC GCGACCTCCT GGGCAAGAAG
    GTGAGCACCA AGACCTTGTC TGAGGAGGAC CTGAAGGAGA TCCCTGCCGA GCAGATGGAT
    TTCCGCGCCA ATCTGCAGCG GCAAGTCAAG CCGAAGACTG TGTCCGAGGA AGAGAGGAAG
    GTGCATAGCC CCCAGCAGGT GGACTTCCGC TCGGTCCTGG CCAAGAAGGG GACGCCCAAG
    ACCCCCGTAC CTGAGAAGCT GCCCCCTCCA AAACCTGCCA CCCCAGATTT TCGCTCGGTG
    TTGGGCAGTA AGAAAAAATT ACCAGCGGAG AATGGTAGCA GCAATGCTGA GCCTTTGAAT
    GTCAAGGCAG CAGAAGGTCC CAAGCCTGTG GGCAATGCCC AGCCTTCAGG CTTTCTCAAA
    CCTATGGGCA ATGCCAAGCT GGCCGAGACC TCCAAACACT TGAGTAATGC CAAGCCAGCC
    GAGCTCCCCA AACCTGTGGG CAACGCCAAG CCAGCCGAGA TCCCCAAACC CGTGGGCAAC
    ACCAGGCCAG CTGAGACCCC CAAAACCGTG GGCAATGCCA AGCCAACTGA GACCTTCAAA
    CCCATGGCCA ATACCAAGCC AGCTGAGACC CTAAAGCCTG TGGGCAATGC CAAGCCAGCT
    GAGACCCTAA AGCCTGTGGG CAATGCCAAG CCAGCTGAGA CCCTAAAGCC CGTGGGCAAT
    GCCAAGCCAG CTGAGACCCC CAAGCCCTTG GGTAATACCA AGCCAGCCGA GCCCCCTAAA
    CCAGCTGGGA AAGAAGAGCT TAAGAAGGAG GTTAAGAATG ATGTGAACTA CAAGAGGGGC
    TCTGCAGGGG CCACAGATAA TGAAAAAAGC CCGGAGAGCC AAGGGTCAGC CCCAACCTTC
    AAGGAAAAGC TACGTGATCT CCGTGTGGCA GAGGGTGAGA AGCTGCTGCT CCAGTGCCAG
    GTATCCTCCG ACCCCCCGGC CACCATCACC TGGACACTGA ATGGGAAGAC ACTCAAGACC
    ACCAAGTTCA TTGTCCTCTC CCAAGAAGGC TCACTGTGCT CCATCTCCAT CGAGAAGGCC
    TTGCCCGAGG ACAGAGGTTT GTACAAGTGT GTGGCCAAGA ACAGCGCCGG CCAGGCTGAG
    TGCTCCTGCC AAGTCACCGT GGACGATGCC CCCACCGGAG AGAATGCCAA GGCCCCGGAG
    ATGAAAGCCC GGAGGCCAAA GAGCTCTCTT CCTCCTGTGC TAGGAACAGA GAGTGATGCC
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCGAAGG CAGCCATGCC CCCTCAGATC
    ATCCAGTTTC CCGAGGACCA AAAGGTGCGA GCGGGAGAGT CTGTGGAGCT GTTTGGTAAA
    GTGGCCGGTA CCCAGCCCAT CACCTGTACC TGGATGAAGT TCCGAAAGCA GATCCAGGAG
    AGTGAGCACA TCAAGGTGGA GAACAGTGAG AATGGCAGCA AGCTCACCAT CCGGGCCGCG
    CGCCAGGAGC ACTGTGGCTG CTACACTCTG CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG
    GCCCAGGTCA ACCTCACTGT TGTGGACAAG CCGGACCCCC CCGCAGGCAC GCCTTGTGCC
    TCTGACATCC GCAGCTCCTC GCTGACCCTG TCCTGGTATG GCTCCTCCTA CGATGGGGGC
    AGCGCCGTAC AGTCCTACAG CGTCGAGATC TGGGACTCAG TGGACAAGAC GTGGAAGGAA
    CTAGCCACAT GCCGCAGCAC CTCTTTTAAC GTCCAGGACC TGCTGTCTGA CCGGGAGTAT
    AAATTCCGTG TGCGTGCAAT CAACATCTAC GGAACCAGTG AGCCGAGCCA GGAGTCTGAA
    CTCACAGCGG TGGGAGAGAA ACCTGAGGAG CCCAAGGATG AAGTGGAGGT GTCAGATGAC
    GATGAGAAGG AGCCTGAGGT CGATTACCGG ACCGTCACGG TCAATACGGA ACAAAAAGTG
    TCTGACTTCT ACGACATTGA AGAGAGACTA GGATCTGGGA AATTTGGACA GGTCTTCCGA
    CTTGTAGAGA AGAAAACTGG AAAAATCTGG GCAGGGAAAT TCTTCAAGGC GTATTCAGCA
    AAAGAGAAAG AGAACATCCG GCAGGAGATC AGCATCATGA ACTGCCTCCA CCACCCCAAG
    CTGGTCCAGT GTGTGGACGC CTTCGAAGAG AAGGCCAACA TCGTCATGGT CCTGGAGATC
    GTGTCCGGGG GCGAGCTGTT CGAGCGCATC ATTGACGAGG ACTTTGAGCT GACGGAGCGC
    GAGTGCATCA AGTACATGCG GCAGATCTCG GAGGGGGTGG AGTACATCCA CAAGCAGGGC
    ATCGTCCACC TGGACCTCAA GCCTGAGAAC ATCATGTGTG TCAACAAGAC GGGCACCCGG
    ATCAAGCTCA TCGACTTCGG TCTGGCCCGG CGGCTGGAGA ATGCGGGGTC TCTGAAGGTC
    CTCTTTGGCA CCCCGGAGTT TGTGGCGCCT GAAGTGATCA ACTATGAGCC CATTGGCTAT
    GCCACGGACA TGTGGAGCAT CGGGGTCATC TGCTACATCC TGGTCAGTGG ACTGTCCCCC
    TTTATGGGTG ACAACGATAA CGAGACCTTA GCCAACGTTA CCTCAGCCAC CTGGGACTTC
    GACGATGAGG CCTTCGATGA GATCTCCGAC GACGCCAAGG ATTTCATCAG CAATTTACTG
    AAGAAAGATA TGAAAAACCG CTTGGACTGC ACCCAGTGCC TCCAGCACCC ATGGCTAATG
    AAAGATACCA AGAACATGGA GGCCAAGAAG CTCTCCAAGG ACCGGATGAA AAAGTACATG
    GCAAGGAGGA AATGGCAGAA AACGGGCAAT GCTGTGAGAG CCATTGGAAG ACTGTCCTCT
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG
    CTGAATGCAG AAAAATTAGA ATCTGAAGAA GATGTCTCCC AAGCTTTCCT TGAGGCCGTC
    GCTGAGGAAA AGCCCCATGT AAAACCCTAT TTTTCTAAGA CCATTCGTGA TTTAGAAGTT
    GTGGAGGGAA GCGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCTGAGGTT
    GTCTGGTTCA AAGATGACCA GTCAATCAGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT
    GAGGATGGGA ACTGCTCTTT AATCATTAGC GATGTCTGCG GTGATGATGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAACAG TCTTGGAGAA GCCACCTGCA CAGCAGAGCT CATTGTGGAA
    ACCATGGAGG AAGGAGAAGG GGAAGGGGAA GAAGAAGAGG AGGAAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020925895.1
    CDS414..6224
    Translation

    Target ORF information:

    RefSeq Version XM_021070236.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa myosin light chain kinase (MYLK), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021070236.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGATCACT CGAGAGTCGG CTCCACGCCC CTGACCGAGG CCCCTGCTTT CATTTTGCCC 
    CCTCGGAACC TTTGCATCAA GGAAGGAGCC ACTGCCAAGT TTGAAGGGAG GGTCCGGGGT
    TACCCAGAGC CCCAGGTGAC ATGGCACAGA AACGGACAGC CCATCACCAG TGGAGGTCGC
    TTCCTGCTGG ACTGTGGCAT CCGGGGGACC TTCAGTCTTG TGATTCATTC TGTCTGTGAG
    GAGGACAAGG GAAAGTATAC CTGTGAAGCC ACCAATGGTA GTGGCGCCCG CCAGGTGACA
    GTGGAGTTGA CGGTAGAAGG GGGCTTTGTG AAGAAGCATG GCCAGCCTGT TGTTTCCAAA
    GCCTTGGGGG ACAGATTTGC AGCCCCTGCA GTCGAGACCC GTCCTAGCAT CTGGGGAGAG
    TGCCCACCAA AGTTTGCCAC CAAGCTGGGC CGAGCGGTGG TCAAAGAAGG ACAGATGGGG
    CGATTCTCCT GCAAGATCAC TGGCCGGCCC CAACCGCAGG TCACCTGGCT CAAGGGAGAT
    GTTCCCCTGC AGCCCAGTGC CCGTGTGACC ATGTCTGAGA AGAATGGGGT GCAAGTGCTG
    GAAATCCACG AAGTCAACCG AGACGACGTG GGGGTGTACA CGTGCATGGT GGTGAATGGG
    TCGGGGAAGG CCTCCATGTC AGCTGAGCTC TCCATCCAAG GTTTGGACAA TACCAATAGG
    TCGCTTGTGA GAGGAACAAA GGCGGCCAGT TCAGACATCA GGACAGAAGC GACCAATGGA
    CTCTCACAGG GGCCAAAGCT GGATGGCCTG GAGGCTGCAG CGGAGAGTAA GAACTGCTCC
    AGCGCCCAGA GGGGTGGCTC CCCGACCTGG GCTTCAGGGA GCCAGCCTCA GCCTCCGAGG
    GAATCCAAGC TGGAGCCGTC AGGGGGCTCA GACAGAAAAG CCCTGAGGAG CCCCATCCTG
    CAGAAGACTT CCAGTGCCAT CACCCTGCAG CCTGCAAAAG TCCAGTTGGA ACCAAGGGCT
    CCAGTCTCAG GCACCCTCTT GCCTTCCAGA GAAGAGAGAG AGCGGCCAGC TGCTCCACCT
    GCCGCCACCC TGCCCTCCAG ACAGTCTGGC ACAGGAGGCC AAGAAGCTGT GAGCAAGGCT
    GCTACCAAGC AGGTTGCCAT GGAGAGCCGA AGGGCTGCCA CAGTCCCCAG ATTCGAGAGC
    AAGCCCCAAA GCCAGGAGGT CAGTGAAGAT CAGCCAGTCA AGTTCAAGTG TGAAGTTTCA
    GGGGTTCCAA AGCCCGAAGT GACCTGGTTC CTGGACGGTG CCCCTGTGAA GAGGCGAGAA
    GGCACCTTTG AGGTTTATGA AGCCGGAGGA TCCCACTACC TCTGCCTGCC AAGAGCCCGG
    GCCAGGGACA GCGGGAACTA CAGCTGCACA GCCACCAACA TCCGAGGCCA GGTGTCCTGT
    ACCTGGACCC TCCTGGTGAA AAGGCAGCCC ATCCAGTACG CCCGCTCCAC CTGCGAGGCC
    GGCGTTGCCA AGCTCCACAT CCAGGATGCC CTCCCGGAGG ACGACGGCAC CTATACCTGT
    CTGGCCGAGA ACACCTTGGG GCGGGTGTCC TGCAGCGCCC GGGTCACTGT CCATGAAAAG
    AAGAGTGATG GGAAGAGTGA GTACCTCCTG CCCGCTGCCC CCAGCAAGCC CATGGCACCC
    ATCTTCCTGC AGGGTCTCTC TGATCTCAGC GTCATGGATG GGAGCCAGGT CACCATGACA
    GTCCAGGTGT CAGGGAACCC ACCCCCTGAG GTCATCTGGC TTCACGATGG GAATGAGATC
    CAGGAGTCGG AGGACTTCCA CTTTGAGCAG AGGGGCACTC GGCACAGCCT CTGTATCCAG
    GAAGTGTTCC CAGAAGACAC AGGCACATAC ACCTGTGAAG CCTGGAACAG CGCAGGAGAG
    GTCCGCACCC AGGCCGTGCT CACGGTGCAA GAGCCTCAGG ACGGCACCCA GCCTTGGTTC
    ATCAGCAAGC CCCGCTCTGT GACAGCCTGC CTGGGCCAGA GTGTCCTCAT CTCCTGTGCC
    ATAGCTGGGG ACCCCTTCCC TACTGTGCAC TGGCTCCGAG ACGGCAAAGC CCTCTCCAAA
    GACACTGGCC ACTTCGAGGT GCTGCAGAAT GAGGACGTGT TCACCCTGGT TCTCAAGAAC
    GTGCAGCCCT GGCACGCCGG CCAGTATGAG ATCCTGCTCA AGAACCGGGT TGGCGAGTGC
    AGCTGCCAGG TGTCCCTGGT GCTACAGAGC AGCCCTGCCA GAGCCCCCCT GCGGGGGAGG
    GAGCCTGCCA GCTGTGAGGG CCAAGGAGCT GGTGCTGATG GTGGTGGTGC TGGCAGAGAC
    GGCTACGGGA GCCTGAGGCC CGGCTGGCCA GCAAGAGGGC AGGGTGAGCC CGAGGACGAA
    GACGGCGAGG ACGTGCGCGG GGTGCTCAAG AGGCGCGTGG AGACGCGGCA GCACACCGAG
    GAGGCTATCC GCCAGCAGGA GGTGGAGCAG CTGGACTTCC GCGACCTCCT GGGCAAGAAG
    GTGAGCACCA AGACCTTGTC TGAGGAGGAC CTGAAGGAGA TCCCTGCCGA GCAGATGGAT
    TTCCGCGCCA ATCTGCAGCG GCAAGTCAAG CCGAAGACTG TGTCCGAGGA AGAGAGGAAG
    GTGCATAGCC CCCAGCAGGT GGACTTCCGC TCGGTCCTGG CCAAGAAGGG GACGCCCAAG
    ACCCCCGTAC CTGAGAAGCT GCCCCCTCCA AAACCTGCCA CCCCAGATTT TCGCTCGGTG
    TTGGGCAGTA AGAAAAAATT ACCAGCGGAG AATGGTAGCA GCAATGCTGA GCCTTTGAAT
    GTCAAGGCAG CAGAAGGTCC CAAGCCTGTG GGCAATGCCC AGCCTTCAGG CTTTCTCAAA
    CCTATGGGCA ATGCCAAGCT GGCCGAGACC TCCAAACACT TGAGTAATGC CAAGCCAGCC
    GAGCTCCCCA AACCTGTGGG CAACGCCAAG CCAGCCGAGA TCCCCAAACC CGTGGGCAAC
    ACCAGGCCAG CTGAGACCCC CAAAACCGTG GGCAATGCCA AGCCAACTGA GACCTTCAAA
    CCCATGGCCA ATACCAAGCC AGCTGAGACC CTAAAGCCTG TGGGCAATGC CAAGCCAGCT
    GAGACCCTAA AGCCTGTGGG CAATGCCAAG CCAGCTGAGA CCCTAAAGCC CGTGGGCAAT
    GCCAAGCCAG CTGAGACCCC CAAGCCCTTG GGTAATACCA AGCCAGCCGA GCCCCCTAAA
    CCAGCTGGGA AAGAAGAGCT TAAGAAGGAG GTTAAGAATG ATGTGAACTA CAAGAGGGGC
    TCTGCAGGGG CCACAGATAA TGAAAAAAGC CCGGAGAGCC AAGGGTCAGC CCCAACCTTC
    AAGGAAAAGC TACGTGATCT CCGTGTGGCA GAGGGTGAGA AGCTGCTGCT CCAGTGCCAG
    GTATCCTCCG ACCCCCCGGC CACCATCACC TGGACACTGA ATGGGAAGAC ACTCAAGACC
    ACCAAGTTCA TTGTCCTCTC CCAAGAAGGC TCACTGTGCT CCATCTCCAT CGAGAAGGCC
    TTGCCCGAGG ACAGAGGTTT GTACAAGTGT GTGGCCAAGA ACAGCGCCGG CCAGGCTGAG
    TGCTCCTGCC AAGTCACCGT GGACGATGCC CCCACCGGAG AGAATGCCAA GGCCCCGGAG
    ATGAAAGCCC GGAGGCCAAA GAGCTCTCTT CCTCCTGTGC TAGGAACAGA GAGTGATGCC
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCGAAGG CAGCCATGCC CCCTCAGATC
    ATCCAGTTTC CCGAGGACCA AAAGGTGCGA GCGGGAGAGT CTGTGGAGCT GTTTGGTAAA
    GTGGCCGGTA CCCAGCCCAT CACCTGTACC TGGATGAAGT TCCGAAAGCA GATCCAGGAG
    AGTGAGCACA TCAAGGTGGA GAACAGTGAG AATGGCAGCA AGCTCACCAT CCGGGCCGCG
    CGCCAGGAGC ACTGTGGCTG CTACACTCTG CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG
    GCCCAGGTCA ACCTCACTGT TGTGGACAAG CCGGACCCCC CCGCAGGCAC GCCTTGTGCC
    TCTGACATCC GCAGCTCCTC GCTGACCCTG TCCTGGTATG GCTCCTCCTA CGATGGGGGC
    AGCGCCGTAC AGTCCTACAG CGTCGAGATC TGGGACTCAG TGGACAAGAC GTGGAAGGAA
    CTAGCCACAT GCCGCAGCAC CTCTTTTAAC GTCCAGGACC TGCTGTCTGA CCGGGAGTAT
    AAATTCCGTG TGCGTGCAAT CAACATCTAC GGAACCAGTG AGCCGAGCCA GGAGTCTGAA
    CTCACAGCGG TGGGAGAGAA ACCTGAGGAG CCCAAGGATG AAGTGGAGGT GTCAGATGAC
    GATGAGAAGG AGCCTGAGGT CGATTACCGG ACCGTCACGG TCAATACGGA ACAAAAAGTG
    TCTGACTTCT ACGACATTGA AGAGAGACTA GGATCTGGGA AATTTGGACA GGTCTTCCGA
    CTTGTAGAGA AGAAAACTGG AAAAATCTGG GCAGGGAAAT TCTTCAAGGC GTATTCAGCA
    AAAGAGAAAG AGAACATCCG GCAGGAGATC AGCATCATGA ACTGCCTCCA CCACCCCAAG
    CTGGTCCAGT GTGTGGACGC CTTCGAAGAG AAGGCCAACA TCGTCATGGT CCTGGAGATC
    GTGTCCGGGG GCGAGCTGTT CGAGCGCATC ATTGACGAGG ACTTTGAGCT GACGGAGCGC
    GAGTGCATCA AGTACATGCG GCAGATCTCG GAGGGGGTGG AGTACATCCA CAAGCAGGGC
    ATCGTCCACC TGGACCTCAA GCCTGAGAAC ATCATGTGTG TCAACAAGAC GGGCACCCGG
    ATCAAGCTCA TCGACTTCGG TCTGGCCCGG CGGCTGGAGA ATGCGGGGTC TCTGAAGGTC
    CTCTTTGGCA CCCCGGAGTT TGTGGCGCCT GAAGTGATCA ACTATGAGCC CATTGGCTAT
    GCCACGGACA TGTGGAGCAT CGGGGTCATC TGCTACATCC TGGTCAGTGG ACTGTCCCCC
    TTTATGGGTG ACAACGATAA CGAGACCTTA GCCAACGTTA CCTCAGCCAC CTGGGACTTC
    GACGATGAGG CCTTCGATGA GATCTCCGAC GACGCCAAGG ATTTCATCAG CAATTTACTG
    AAGAAAGATA TGAAAAACCG CTTGGACTGC ACCCAGTGCC TCCAGCACCC ATGGCTAATG
    AAAGATACCA AGAACATGGA GGCCAAGAAG CTCTCCAAGG ACCGGATGAA AAAGTACATG
    GCAAGGAGGA AATGGCAGAA AACGGGCAAT GCTGTGAGAG CCATTGGAAG ACTGTCCTCT
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG
    CTGAATGCAG AAAAATTAGA ATCTGAAGAA GATGTCTCCC AAGCTTTCCT TGAGGCCGTC
    GCTGAGGAAA AGCCCCATGT AAAACCCTAT TTTTCTAAGA CCATTCGTGA TTTAGAAGTT
    GTGGAGGGAA GCGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCTGAGGTT
    GTCTGGTTCA AAGATGACCA GTCAATCAGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT
    GAGGATGGGA ACTGCTCTTT AATCATTAGC GATGTCTGCG GTGATGATGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAACAG TCTTGGAGAA GCCACCTGCA CAGCAGAGCT CATTGTGGAA
    ACCATGGAGG AAGGAGAAGG GGAAGGGGAA GAAGAAGAGG AGGAAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe236097
    Clone ID Related Accession (Same CDS sequence) XM_021070238.1
    Accession Version XM_021070238.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5472bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product myosin light chain kinase, smooth muscle isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010455.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGGGCGAT TCTCCTGCAA GATCACTGGC CGGCCCCAAC CGCAGGTCAC CTGGCTCAAG 
    GGAGATGTTC CCCTGCAGCC CAGTGCCCGT GTGACCATGT CTGAGAAGAA TGGGGTGCAA
    GTGCTGGAAA TCCACGAAGT CAACCGAGAC GACGTGGGGG TGTACACGTG CATGGTGGTG
    AATGGGTCGG GGAAGGCCTC CATGTCAGCT GAGCTCTCCA TCCAAGGTTT GGACAATACC
    AATAGGTCGC TTGTGAGAGG AACAAAGGCG GCCAGTTCAG ACATCAGGAC AGAAGCGACC
    AATGGACTCT CACAGGGGCC AAAGCTGGAT GGCCTGGAGG CTGCAGCGGA GAGTAAGAAC
    TGCTCCAGCG CCCAGAGGGG TGGCTCCCCG ACCTGGGCTT CAGGGAGCCA GCCTCAGCCT
    CCGAGGGAAT CCAAGCTGGA GCCGTCAGGG GGCTCAGACA GAAAAGCCCT GAGGAGCCCC
    ATCCTGCAGA AGACTTCCAG TGCCATCACC CTGCAGCCTG CAAAAGTCCA GTTGGAACCA
    AGGGCTCCAG TCTCAGGCAC CCTCTTGCCT TCCAGAGAAG AGAGAGAGCG GCCAGCTGCT
    CCACCTGCCG CCACCCTGCC CTCCAGACAG TCTGGCACAG GAGGCCAAGA AGCTGTGAGC
    AAGGCTGCTA CCAAGCAGGT TGCCATGGAG AGCCGAAGGG CTGCCACAGT CCCCAGATTC
    GAGAGCAAGC CCCAAAGCCA GGAGGTCAGT GAAGATCAGC CAGTCAAGTT CAAGTGTGAA
    GTTTCAGGGG TTCCAAAGCC CGAAGTGACC TGGTTCCTGG ACGGTGCCCC TGTGAAGAGG
    CGAGAAGGCA CCTTTGAGGT TTATGAAGCC GGAGGATCCC ACTACCTCTG CCTGCCAAGA
    GCCCGGGCCA GGGACAGCGG GAACTACAGC TGCACAGCCA CCAACATCCG AGGCCAGGTG
    TCCTGTACCT GGACCCTCCT GGTGAAAAGG CTGGCCGTGA CAGAGGCGCC CCCGTCCTTC
    TCCAGTGTCC TGAAGGACTG TGCTGTGGTT GAGGGCCAGG ACTTCGTGCT GCAGTGCTCC
    GTGCAGGGGA CCCCGCTGCC CCAGATCACT TGGCTGCTCA ATGGGCAGCC CATCCAGTAC
    GCCCGCTCCA CCTGCGAGGC CGGCGTTGCC AAGCTCCACA TCCAGGATGC CCTCCCGGAG
    GACGACGGCA CCTATACCTG TCTGGCCGAG AACACCTTGG GGCGGGTGTC CTGCAGCGCC
    CGGGTCACTG TCCATGAAAA GAAGAGTGAT GGGAAGAGTG AGTACCTCCT GCCCGCTGCC
    CCCAGCAAGC CCATGGCACC CATCTTCCTG CAGGGTCTCT CTGATCTCAG CGTCATGGAT
    GGGAGCCAGG TCACCATGAC AGTCCAGGTG TCAGGGAACC CACCCCCTGA GGTCATCTGG
    CTTCACGATG GGAATGAGAT CCAGGAGTCG GAGGACTTCC ACTTTGAGCA GAGGGGCACT
    CGGCACAGCC TCTGTATCCA GGAAGTGTTC CCAGAAGACA CAGGCACATA CACCTGTGAA
    GCCTGGAACA GCGCAGGAGA GGTCCGCACC CAGGCCGTGC TCACGGTGCA AGAGCCTCAG
    GACGGCACCC AGCCTTGGTT CATCAGCAAG CCCCGCTCTG TGACAGCCTG CCTGGGCCAG
    AGTGTCCTCA TCTCCTGTGC CATAGCTGGG GACCCCTTCC CTACTGTGCA CTGGCTCCGA
    GACGGCAAAG CCCTCTCCAA AGACACTGGC CACTTCGAGG TGCTGCAGAA TGAGGACGTG
    TTCACCCTGG TTCTCAAGAA CGTGCAGCCC TGGCACGCCG GCCAGTATGA GATCCTGCTC
    AAGAACCGGG TTGGCGAGTG CAGCTGCCAG GTGTCCCTGG TGCTACAGAG CAGCCCTGCC
    AGAGCCCCCC TGCGGGGGAG GGAGCCTGCC AGCTGTGAGG GCCAAGGAGC TGGTGCTGAT
    GGTGGTGGTG CTGGCAGAGA CGGCTACGGG AGCCTGAGGC CCGGCTGGCC AGCAAGAGGG
    CAGGGTGAGC CCGAGGACGA AGACGGCGAG GACGTGCGCG GGGTGCTCAA GAGGCGCGTG
    GAGACGCGGC AGCACACCGA GGAGGCTATC CGCCAGCAGG AGGTGGAGCA GCTGGACTTC
    CGCGACCTCC TGGGCAAGAA GGTGAGCACC AAGACCTTGT CTGAGGAGGA CCTGAAGGAG
    ATCCCTGCCG AGCAGATGGA TTTCCGCGCC AATCTGCAGC GGCAAGTCAA GCCGAAGACT
    GTGTCCGAGG AAGAGAGGAA GGTGCATAGC CCCCAGCAGG TGGACTTCCG CTCGGTCCTG
    GCCAAGAAGG GGACGCCCAA GACCCCCGTA CCTGAGAAGC TGCCCCCTCC AAAACCTGCC
    ACCCCAGATT TTCGCTCGGT GTTGGGCAGT AAGAAAAAAT TACCAGCGGA GAATGGTAGC
    AGCAATGCTG AGCCTTTGAA TGTCAAGGCA GCAGAAGGTC CCAAGCCTGT GGGCAATGCC
    CAGCCTTCAG GCTTTCTCAA ACCTATGGGC AATGCCAAGC TGGCCGAGAC CTCCAAACAC
    TTGAGTAATG CCAAGCCAGC CGAGCTCCCC AAACCTGTGG GCAACGCCAA GCCAGCCGAG
    ATCCCCAAAC CCGTGGGCAA CACCAGGCCA GCTGAGACCC CCAAAACCGT GGGCAATGCC
    AAGCCAACTG AGACCTTCAA ACCCATGGCC AATACCAAGC CAGCTGAGAC CCTAAAGCCT
    GTGGGCAATG CCAAGCCAGC TGAGACCCTA AAGCCTGTGG GCAATGCCAA GCCAGCTGAG
    ACCCTAAAGC CCGTGGGCAA TGCCAAGCCA GCTGAGACCC CCAAGCCCTT GGGTAATACC
    AAGCCAGCCG AGCCCCCTAA ACCAGCTGGG AAAGAAGAGC TTAAGAAGGA GGTTAAGAAT
    GATGTGAACT ACAAGAGGGG CTCTGCAGGG GCCACAGATA ATGAAAAAAG CCCGGAGAGC
    CAAGGGTCAG CCCCAACCTT CAAGGAAAAG CTACGTGATC TCCGTGTGGC AGAGGGTGAG
    AAGCTGCTGC TCCAGTGCCA GGTATCCTCC GACCCCCCGG CCACCATCAC CTGGACACTG
    AATGGGAAGA CACTCAAGAC CACCAAGTTC ATTGTCCTCT CCCAAGAAGG CTCACTGTGC
    TCCATCTCCA TCGAGAAGGC CTTGCCCGAG GACAGAGGTT TGTACAAGTG TGTGGCCAAG
    AACAGCGCCG GCCAGGCTGA GTGCTCCTGC CAAGTCACCG TGGACGATGC CCCCACCGGA
    GAGAATGCCA AGGCCCCGGA GATGAAAGCC CGGAGGCCAA AGAGCTCTCT TCCTCCTGTG
    CTAGGAACAG AGAGTGATGC CACTGTGAAA AAGAAACCTG CCCCCAAGAC ACCTCCGAAG
    GCAGCCATGC CCCCTCAGAT CATCCAGTTT CCCGAGGACC AAAAGGTGCG AGCGGGAGAG
    TCTGTGGAGC TGTTTGGTAA AGTGGCCGGT ACCCAGCCCA TCACCTGTAC CTGGATGAAG
    TTCCGAAAGC AGATCCAGGA GAGTGAGCAC ATCAAGGTGG AGAACAGTGA GAATGGCAGC
    AAGCTCACCA TCCGGGCCGC GCGCCAGGAG CACTGTGGCT GCTACACTCT GCTGGTGGAG
    AACAAGCTGG GCAGCAGGCA GGCCCAGGTC AACCTCACTG TTGTGGACAA GCCGGACCCC
    CCCGCAGGCA CGCCTTGTGC CTCTGACATC CGCAGCTCCT CGCTGACCCT GTCCTGGTAT
    GGCTCCTCCT ACGATGGGGG CAGCGCCGTA CAGTCCTACA GCGTCGAGAT CTGGGACTCA
    GTGGACAAGA CGTGGAAGGA ACTAGCCACA TGCCGCAGCA CCTCTTTTAA CGTCCAGGAC
    CTGCTGTCTG ACCGGGAGTA TAAATTCCGT GTGCGTGCAA TCAACATCTA CGGAACCAGT
    GAGCCGAGCC AGGAGTCTGA ACTCACAGCG GTGGGAGAGA AACCTGAGGA GCCCAAGGAT
    GAAGTGGAGG TGTCAGATGA CGATGAGAAG GAGCCTGAGG TCGATTACCG GACCGTCACG
    GTCAATACGG AACAAAAAGT GTCTGACTTC TACGACATTG AAGAGAGACT AGGATCTGGG
    AAATTTGGAC AGGTCTTCCG ACTTGTAGAG AAGAAAACTG GAAAAATCTG GGCAGGGAAA
    TTCTTCAAGG CGTATTCAGC AAAAGAGAAA GAGAACATCC GGCAGGAGAT CAGCATCATG
    AACTGCCTCC ACCACCCCAA GCTGGTCCAG TGTGTGGACG CCTTCGAAGA GAAGGCCAAC
    ATCGTCATGG TCCTGGAGAT CGTGTCCGGG GGCGAGCTGT TCGAGCGCAT CATTGACGAG
    GACTTTGAGC TGACGGAGCG CGAGTGCATC AAGTACATGC GGCAGATCTC GGAGGGGGTG
    GAGTACATCC ACAAGCAGGG CATCGTCCAC CTGGACCTCA AGCCTGAGAA CATCATGTGT
    GTCAACAAGA CGGGCACCCG GATCAAGCTC ATCGACTTCG GTCTGGCCCG GCGGCTGGAG
    AATGCGGGGT CTCTGAAGGT CCTCTTTGGC ACCCCGGAGT TTGTGGCGCC TGAAGTGATC
    AACTATGAGC CCATTGGCTA TGCCACGGAC ATGTGGAGCA TCGGGGTCAT CTGCTACATC
    CTGGTCAGTG GACTGTCCCC CTTTATGGGT GACAACGATA ACGAGACCTT AGCCAACGTT
    ACCTCAGCCA CCTGGGACTT CGACGATGAG GCCTTCGATG AGATCTCCGA CGACGCCAAG
    GATTTCATCA GCAATTTACT GAAGAAAGAT ATGAAAAACC GCTTGGACTG CACCCAGTGC
    CTCCAGCACC CATGGCTAAT GAAAGATACC AAGAACATGG AGGCCAAGAA GCTCTCCAAG
    GACCGGATGA AAAAGTACAT GGCAAGGAGG AAATGGCAGA AAACGGGCAA TGCTGTGAGA
    GCCATTGGAA GACTGTCCTC TATGGCAATG ATCTCAGGGC TCAGTGGCAG GAAATCCTCA
    ACAGGGTCAC CAACCAGCCC GCTGAATGCA GAAAAATTAG AATCTGAAGA AGATGTCTCC
    CAAGCTTTCC TTGAGGCCGT CGCTGAGGAA AAGCCCCATG TAAAACCCTA TTTTTCTAAG
    ACCATTCGTG ATTTAGAAGT TGTGGAGGGA AGCGCTGCTA GATTTGACTG CAAGATTGAA
    GGATACCCAG ACCCTGAGGT TGTCTGGTTC AAAGATGACC AGTCAATCAG GGAGTCCCGC
    CACTTCCAGA TAGACTACGA TGAGGATGGG AACTGCTCTT TAATCATTAG CGATGTCTGC
    GGTGATGATG ATGCCAAGTA CACCTGCAAG GCTGTCAACA GTCTTGGAGA AGCCACCTGC
    ACAGCAGAGC TCATTGTGGA AACCATGGAG GAAGGAGAAG GGGAAGGGGA AGAAGAAGAG
    GAGGAAGAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020925897.1
    CDS511..5982
    Translation

    Target ORF information:

    RefSeq Version XM_021070238.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa myosin light chain kinase (MYLK), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021070238.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGGGCGAT TCTCCTGCAA GATCACTGGC CGGCCCCAAC CGCAGGTCAC CTGGCTCAAG 
    GGAGATGTTC CCCTGCAGCC CAGTGCCCGT GTGACCATGT CTGAGAAGAA TGGGGTGCAA
    GTGCTGGAAA TCCACGAAGT CAACCGAGAC GACGTGGGGG TGTACACGTG CATGGTGGTG
    AATGGGTCGG GGAAGGCCTC CATGTCAGCT GAGCTCTCCA TCCAAGGTTT GGACAATACC
    AATAGGTCGC TTGTGAGAGG AACAAAGGCG GCCAGTTCAG ACATCAGGAC AGAAGCGACC
    AATGGACTCT CACAGGGGCC AAAGCTGGAT GGCCTGGAGG CTGCAGCGGA GAGTAAGAAC
    TGCTCCAGCG CCCAGAGGGG TGGCTCCCCG ACCTGGGCTT CAGGGAGCCA GCCTCAGCCT
    CCGAGGGAAT CCAAGCTGGA GCCGTCAGGG GGCTCAGACA GAAAAGCCCT GAGGAGCCCC
    ATCCTGCAGA AGACTTCCAG TGCCATCACC CTGCAGCCTG CAAAAGTCCA GTTGGAACCA
    AGGGCTCCAG TCTCAGGCAC CCTCTTGCCT TCCAGAGAAG AGAGAGAGCG GCCAGCTGCT
    CCACCTGCCG CCACCCTGCC CTCCAGACAG TCTGGCACAG GAGGCCAAGA AGCTGTGAGC
    AAGGCTGCTA CCAAGCAGGT TGCCATGGAG AGCCGAAGGG CTGCCACAGT CCCCAGATTC
    GAGAGCAAGC CCCAAAGCCA GGAGGTCAGT GAAGATCAGC CAGTCAAGTT CAAGTGTGAA
    GTTTCAGGGG TTCCAAAGCC CGAAGTGACC TGGTTCCTGG ACGGTGCCCC TGTGAAGAGG
    CGAGAAGGCA CCTTTGAGGT TTATGAAGCC GGAGGATCCC ACTACCTCTG CCTGCCAAGA
    GCCCGGGCCA GGGACAGCGG GAACTACAGC TGCACAGCCA CCAACATCCG AGGCCAGGTG
    TCCTGTACCT GGACCCTCCT GGTGAAAAGG CTGGCCGTGA CAGAGGCGCC CCCGTCCTTC
    TCCAGTGTCC TGAAGGACTG TGCTGTGGTT GAGGGCCAGG ACTTCGTGCT GCAGTGCTCC
    GTGCAGGGGA CCCCGCTGCC CCAGATCACT TGGCTGCTCA ATGGGCAGCC CATCCAGTAC
    GCCCGCTCCA CCTGCGAGGC CGGCGTTGCC AAGCTCCACA TCCAGGATGC CCTCCCGGAG
    GACGACGGCA CCTATACCTG TCTGGCCGAG AACACCTTGG GGCGGGTGTC CTGCAGCGCC
    CGGGTCACTG TCCATGAAAA GAAGAGTGAT GGGAAGAGTG AGTACCTCCT GCCCGCTGCC
    CCCAGCAAGC CCATGGCACC CATCTTCCTG CAGGGTCTCT CTGATCTCAG CGTCATGGAT
    GGGAGCCAGG TCACCATGAC AGTCCAGGTG TCAGGGAACC CACCCCCTGA GGTCATCTGG
    CTTCACGATG GGAATGAGAT CCAGGAGTCG GAGGACTTCC ACTTTGAGCA GAGGGGCACT
    CGGCACAGCC TCTGTATCCA GGAAGTGTTC CCAGAAGACA CAGGCACATA CACCTGTGAA
    GCCTGGAACA GCGCAGGAGA GGTCCGCACC CAGGCCGTGC TCACGGTGCA AGAGCCTCAG
    GACGGCACCC AGCCTTGGTT CATCAGCAAG CCCCGCTCTG TGACAGCCTG CCTGGGCCAG
    AGTGTCCTCA TCTCCTGTGC CATAGCTGGG GACCCCTTCC CTACTGTGCA CTGGCTCCGA
    GACGGCAAAG CCCTCTCCAA AGACACTGGC CACTTCGAGG TGCTGCAGAA TGAGGACGTG
    TTCACCCTGG TTCTCAAGAA CGTGCAGCCC TGGCACGCCG GCCAGTATGA GATCCTGCTC
    AAGAACCGGG TTGGCGAGTG CAGCTGCCAG GTGTCCCTGG TGCTACAGAG CAGCCCTGCC
    AGAGCCCCCC TGCGGGGGAG GGAGCCTGCC AGCTGTGAGG GCCAAGGAGC TGGTGCTGAT
    GGTGGTGGTG CTGGCAGAGA CGGCTACGGG AGCCTGAGGC CCGGCTGGCC AGCAAGAGGG
    CAGGGTGAGC CCGAGGACGA AGACGGCGAG GACGTGCGCG GGGTGCTCAA GAGGCGCGTG
    GAGACGCGGC AGCACACCGA GGAGGCTATC CGCCAGCAGG AGGTGGAGCA GCTGGACTTC
    CGCGACCTCC TGGGCAAGAA GGTGAGCACC AAGACCTTGT CTGAGGAGGA CCTGAAGGAG
    ATCCCTGCCG AGCAGATGGA TTTCCGCGCC AATCTGCAGC GGCAAGTCAA GCCGAAGACT
    GTGTCCGAGG AAGAGAGGAA GGTGCATAGC CCCCAGCAGG TGGACTTCCG CTCGGTCCTG
    GCCAAGAAGG GGACGCCCAA GACCCCCGTA CCTGAGAAGC TGCCCCCTCC AAAACCTGCC
    ACCCCAGATT TTCGCTCGGT GTTGGGCAGT AAGAAAAAAT TACCAGCGGA GAATGGTAGC
    AGCAATGCTG AGCCTTTGAA TGTCAAGGCA GCAGAAGGTC CCAAGCCTGT GGGCAATGCC
    CAGCCTTCAG GCTTTCTCAA ACCTATGGGC AATGCCAAGC TGGCCGAGAC CTCCAAACAC
    TTGAGTAATG CCAAGCCAGC CGAGCTCCCC AAACCTGTGG GCAACGCCAA GCCAGCCGAG
    ATCCCCAAAC CCGTGGGCAA CACCAGGCCA GCTGAGACCC CCAAAACCGT GGGCAATGCC
    AAGCCAACTG AGACCTTCAA ACCCATGGCC AATACCAAGC CAGCTGAGAC CCTAAAGCCT
    GTGGGCAATG CCAAGCCAGC TGAGACCCTA AAGCCTGTGG GCAATGCCAA GCCAGCTGAG
    ACCCTAAAGC CCGTGGGCAA TGCCAAGCCA GCTGAGACCC CCAAGCCCTT GGGTAATACC
    AAGCCAGCCG AGCCCCCTAA ACCAGCTGGG AAAGAAGAGC TTAAGAAGGA GGTTAAGAAT
    GATGTGAACT ACAAGAGGGG CTCTGCAGGG GCCACAGATA ATGAAAAAAG CCCGGAGAGC
    CAAGGGTCAG CCCCAACCTT CAAGGAAAAG CTACGTGATC TCCGTGTGGC AGAGGGTGAG
    AAGCTGCTGC TCCAGTGCCA GGTATCCTCC GACCCCCCGG CCACCATCAC CTGGACACTG
    AATGGGAAGA CACTCAAGAC CACCAAGTTC ATTGTCCTCT CCCAAGAAGG CTCACTGTGC
    TCCATCTCCA TCGAGAAGGC CTTGCCCGAG GACAGAGGTT TGTACAAGTG TGTGGCCAAG
    AACAGCGCCG GCCAGGCTGA GTGCTCCTGC CAAGTCACCG TGGACGATGC CCCCACCGGA
    GAGAATGCCA AGGCCCCGGA GATGAAAGCC CGGAGGCCAA AGAGCTCTCT TCCTCCTGTG
    CTAGGAACAG AGAGTGATGC CACTGTGAAA AAGAAACCTG CCCCCAAGAC ACCTCCGAAG
    GCAGCCATGC CCCCTCAGAT CATCCAGTTT CCCGAGGACC AAAAGGTGCG AGCGGGAGAG
    TCTGTGGAGC TGTTTGGTAA AGTGGCCGGT ACCCAGCCCA TCACCTGTAC CTGGATGAAG
    TTCCGAAAGC AGATCCAGGA GAGTGAGCAC ATCAAGGTGG AGAACAGTGA GAATGGCAGC
    AAGCTCACCA TCCGGGCCGC GCGCCAGGAG CACTGTGGCT GCTACACTCT GCTGGTGGAG
    AACAAGCTGG GCAGCAGGCA GGCCCAGGTC AACCTCACTG TTGTGGACAA GCCGGACCCC
    CCCGCAGGCA CGCCTTGTGC CTCTGACATC CGCAGCTCCT CGCTGACCCT GTCCTGGTAT
    GGCTCCTCCT ACGATGGGGG CAGCGCCGTA CAGTCCTACA GCGTCGAGAT CTGGGACTCA
    GTGGACAAGA CGTGGAAGGA ACTAGCCACA TGCCGCAGCA CCTCTTTTAA CGTCCAGGAC
    CTGCTGTCTG ACCGGGAGTA TAAATTCCGT GTGCGTGCAA TCAACATCTA CGGAACCAGT
    GAGCCGAGCC AGGAGTCTGA ACTCACAGCG GTGGGAGAGA AACCTGAGGA GCCCAAGGAT
    GAAGTGGAGG TGTCAGATGA CGATGAGAAG GAGCCTGAGG TCGATTACCG GACCGTCACG
    GTCAATACGG AACAAAAAGT GTCTGACTTC TACGACATTG AAGAGAGACT AGGATCTGGG
    AAATTTGGAC AGGTCTTCCG ACTTGTAGAG AAGAAAACTG GAAAAATCTG GGCAGGGAAA
    TTCTTCAAGG CGTATTCAGC AAAAGAGAAA GAGAACATCC GGCAGGAGAT CAGCATCATG
    AACTGCCTCC ACCACCCCAA GCTGGTCCAG TGTGTGGACG CCTTCGAAGA GAAGGCCAAC
    ATCGTCATGG TCCTGGAGAT CGTGTCCGGG GGCGAGCTGT TCGAGCGCAT CATTGACGAG
    GACTTTGAGC TGACGGAGCG CGAGTGCATC AAGTACATGC GGCAGATCTC GGAGGGGGTG
    GAGTACATCC ACAAGCAGGG CATCGTCCAC CTGGACCTCA AGCCTGAGAA CATCATGTGT
    GTCAACAAGA CGGGCACCCG GATCAAGCTC ATCGACTTCG GTCTGGCCCG GCGGCTGGAG
    AATGCGGGGT CTCTGAAGGT CCTCTTTGGC ACCCCGGAGT TTGTGGCGCC TGAAGTGATC
    AACTATGAGC CCATTGGCTA TGCCACGGAC ATGTGGAGCA TCGGGGTCAT CTGCTACATC
    CTGGTCAGTG GACTGTCCCC CTTTATGGGT GACAACGATA ACGAGACCTT AGCCAACGTT
    ACCTCAGCCA CCTGGGACTT CGACGATGAG GCCTTCGATG AGATCTCCGA CGACGCCAAG
    GATTTCATCA GCAATTTACT GAAGAAAGAT ATGAAAAACC GCTTGGACTG CACCCAGTGC
    CTCCAGCACC CATGGCTAAT GAAAGATACC AAGAACATGG AGGCCAAGAA GCTCTCCAAG
    GACCGGATGA AAAAGTACAT GGCAAGGAGG AAATGGCAGA AAACGGGCAA TGCTGTGAGA
    GCCATTGGAA GACTGTCCTC TATGGCAATG ATCTCAGGGC TCAGTGGCAG GAAATCCTCA
    ACAGGGTCAC CAACCAGCCC GCTGAATGCA GAAAAATTAG AATCTGAAGA AGATGTCTCC
    CAAGCTTTCC TTGAGGCCGT CGCTGAGGAA AAGCCCCATG TAAAACCCTA TTTTTCTAAG
    ACCATTCGTG ATTTAGAAGT TGTGGAGGGA AGCGCTGCTA GATTTGACTG CAAGATTGAA
    GGATACCCAG ACCCTGAGGT TGTCTGGTTC AAAGATGACC AGTCAATCAG GGAGTCCCGC
    CACTTCCAGA TAGACTACGA TGAGGATGGG AACTGCTCTT TAATCATTAG CGATGTCTGC
    GGTGATGATG ATGCCAAGTA CACCTGCAAG GCTGTCAACA GTCTTGGAGA AGCCACCTGC
    ACAGCAGAGC TCATTGTGGA AACCATGGAG GAAGGAGAAG GGGAAGGGGA AGAAGAAGAG
    GAGGAAGAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe236098
    Clone ID Related Accession (Same CDS sequence) XM_021070239.1
    Accession Version XM_021070239.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5337bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product myosin light chain kinase, smooth muscle isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010455.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGGGCGAT TCTCCTGCAA GATCACTGGC CGGCCCCAAC CGCAGGTCAC CTGGCTCAAG 
    GGAGATGTTC CCCTGCAGCC CAGTGCCCGT GTGACCATGT CTGAGAAGAA TGGGGTGCAA
    GTGCTGGAAA TCCACGAAGT CAACCGAGAC GACGTGGGGG TGTACACGTG CATGGTGGTG
    AATGGGTCGG GGAAGGCCTC CATGTCAGCT GAGCTCTCCA TCCAAGGTTT GGACAATACC
    AATAGGTCGC TTGTGAGAGG AACAAAGGCG GCCAGTTCAG ACATCAGGAC AGAAGCGACC
    AATGGACTCT CACAGGGGCC AAAGCTGGAT GGCCTGGAGG CTGCAGCGGA GAGTAAGAAC
    TGCTCCAGCG CCCAGAGGGG TGGCTCCCCG ACCTGGGCTT CAGGGAGCCA GCCTCAGCCT
    CCGAGGGAAT CCAAGCTGGA GCCGTCAGGG GGCTCAGACA GAAAAGCCCT GAGGAGCCCC
    ATCCTGCAGA AGACTTCCAG TGCCATCACC CTGCAGCCTG CAAAAGTCCA GTTGGAACCA
    AGGGCTCCAG TCTCAGGCAC CCTCTTGCCT TCCAGAGAAG AGAGAGAGCG GCCAGCTGCT
    CCACCTGCCG CCACCCTGCC CTCCAGACAG TCTGGCACAG GAGGCCAAGA AGCTGTGAGC
    AAGGCTGCTA CCAAGCAGGT TGCCATGGAG AGCCGAAGGG CTGCCACAGT CCCCAGATTC
    GAGAGCAAGC CCCAAAGCCA GGAGGTCAGT GAAGATCAGC CAGTCAAGTT CAAGTGTGAA
    GTTTCAGGGG TTCCAAAGCC CGAAGTGACC TGGTTCCTGG ACGGTGCCCC TGTGAAGAGG
    CGAGAAGGCA CCTTTGAGGT TTATGAAGCC GGAGGATCCC ACTACCTCTG CCTGCCAAGA
    GCCCGGGCCA GGGACAGCGG GAACTACAGC TGCACAGCCA CCAACATCCG AGGCCAGGTG
    TCCTGTACCT GGACCCTCCT GGTGAAAAGG CAGCCCATCC AGTACGCCCG CTCCACCTGC
    GAGGCCGGCG TTGCCAAGCT CCACATCCAG GATGCCCTCC CGGAGGACGA CGGCACCTAT
    ACCTGTCTGG CCGAGAACAC CTTGGGGCGG GTGTCCTGCA GCGCCCGGGT CACTGTCCAT
    GAAAAGAAGA GTGATGGGAA GAGTGAGTAC CTCCTGCCCG CTGCCCCCAG CAAGCCCATG
    GCACCCATCT TCCTGCAGGG TCTCTCTGAT CTCAGCGTCA TGGATGGGAG CCAGGTCACC
    ATGACAGTCC AGGTGTCAGG GAACCCACCC CCTGAGGTCA TCTGGCTTCA CGATGGGAAT
    GAGATCCAGG AGTCGGAGGA CTTCCACTTT GAGCAGAGGG GCACTCGGCA CAGCCTCTGT
    ATCCAGGAAG TGTTCCCAGA AGACACAGGC ACATACACCT GTGAAGCCTG GAACAGCGCA
    GGAGAGGTCC GCACCCAGGC CGTGCTCACG GTGCAAGAGC CTCAGGACGG CACCCAGCCT
    TGGTTCATCA GCAAGCCCCG CTCTGTGACA GCCTGCCTGG GCCAGAGTGT CCTCATCTCC
    TGTGCCATAG CTGGGGACCC CTTCCCTACT GTGCACTGGC TCCGAGACGG CAAAGCCCTC
    TCCAAAGACA CTGGCCACTT CGAGGTGCTG CAGAATGAGG ACGTGTTCAC CCTGGTTCTC
    AAGAACGTGC AGCCCTGGCA CGCCGGCCAG TATGAGATCC TGCTCAAGAA CCGGGTTGGC
    GAGTGCAGCT GCCAGGTGTC CCTGGTGCTA CAGAGCAGCC CTGCCAGAGC CCCCCTGCGG
    GGGAGGGAGC CTGCCAGCTG TGAGGGCCAA GGAGCTGGTG CTGATGGTGG TGGTGCTGGC
    AGAGACGGCT ACGGGAGCCT GAGGCCCGGC TGGCCAGCAA GAGGGCAGGG TGAGCCCGAG
    GACGAAGACG GCGAGGACGT GCGCGGGGTG CTCAAGAGGC GCGTGGAGAC GCGGCAGCAC
    ACCGAGGAGG CTATCCGCCA GCAGGAGGTG GAGCAGCTGG ACTTCCGCGA CCTCCTGGGC
    AAGAAGGTGA GCACCAAGAC CTTGTCTGAG GAGGACCTGA AGGAGATCCC TGCCGAGCAG
    ATGGATTTCC GCGCCAATCT GCAGCGGCAA GTCAAGCCGA AGACTGTGTC CGAGGAAGAG
    AGGAAGGTGC ATAGCCCCCA GCAGGTGGAC TTCCGCTCGG TCCTGGCCAA GAAGGGGACG
    CCCAAGACCC CCGTACCTGA GAAGCTGCCC CCTCCAAAAC CTGCCACCCC AGATTTTCGC
    TCGGTGTTGG GCAGTAAGAA AAAATTACCA GCGGAGAATG GTAGCAGCAA TGCTGAGCCT
    TTGAATGTCA AGGCAGCAGA AGGTCCCAAG CCTGTGGGCA ATGCCCAGCC TTCAGGCTTT
    CTCAAACCTA TGGGCAATGC CAAGCTGGCC GAGACCTCCA AACACTTGAG TAATGCCAAG
    CCAGCCGAGC TCCCCAAACC TGTGGGCAAC GCCAAGCCAG CCGAGATCCC CAAACCCGTG
    GGCAACACCA GGCCAGCTGA GACCCCCAAA ACCGTGGGCA ATGCCAAGCC AACTGAGACC
    TTCAAACCCA TGGCCAATAC CAAGCCAGCT GAGACCCTAA AGCCTGTGGG CAATGCCAAG
    CCAGCTGAGA CCCTAAAGCC TGTGGGCAAT GCCAAGCCAG CTGAGACCCT AAAGCCCGTG
    GGCAATGCCA AGCCAGCTGA GACCCCCAAG CCCTTGGGTA ATACCAAGCC AGCCGAGCCC
    CCTAAACCAG CTGGGAAAGA AGAGCTTAAG AAGGAGGTTA AGAATGATGT GAACTACAAG
    AGGGGCTCTG CAGGGGCCAC AGATAATGAA AAAAGCCCGG AGAGCCAAGG GTCAGCCCCA
    ACCTTCAAGG AAAAGCTACG TGATCTCCGT GTGGCAGAGG GTGAGAAGCT GCTGCTCCAG
    TGCCAGGTAT CCTCCGACCC CCCGGCCACC ATCACCTGGA CACTGAATGG GAAGACACTC
    AAGACCACCA AGTTCATTGT CCTCTCCCAA GAAGGCTCAC TGTGCTCCAT CTCCATCGAG
    AAGGCCTTGC CCGAGGACAG AGGTTTGTAC AAGTGTGTGG CCAAGAACAG CGCCGGCCAG
    GCTGAGTGCT CCTGCCAAGT CACCGTGGAC GATGCCCCCA CCGGAGAGAA TGCCAAGGCC
    CCGGAGATGA AAGCCCGGAG GCCAAAGAGC TCTCTTCCTC CTGTGCTAGG AACAGAGAGT
    GATGCCACTG TGAAAAAGAA ACCTGCCCCC AAGACACCTC CGAAGGCAGC CATGCCCCCT
    CAGATCATCC AGTTTCCCGA GGACCAAAAG GTGCGAGCGG GAGAGTCTGT GGAGCTGTTT
    GGTAAAGTGG CCGGTACCCA GCCCATCACC TGTACCTGGA TGAAGTTCCG AAAGCAGATC
    CAGGAGAGTG AGCACATCAA GGTGGAGAAC AGTGAGAATG GCAGCAAGCT CACCATCCGG
    GCCGCGCGCC AGGAGCACTG TGGCTGCTAC ACTCTGCTGG TGGAGAACAA GCTGGGCAGC
    AGGCAGGCCC AGGTCAACCT CACTGTTGTG GACAAGCCGG ACCCCCCCGC AGGCACGCCT
    TGTGCCTCTG ACATCCGCAG CTCCTCGCTG ACCCTGTCCT GGTATGGCTC CTCCTACGAT
    GGGGGCAGCG CCGTACAGTC CTACAGCGTC GAGATCTGGG ACTCAGTGGA CAAGACGTGG
    AAGGAACTAG CCACATGCCG CAGCACCTCT TTTAACGTCC AGGACCTGCT GTCTGACCGG
    GAGTATAAAT TCCGTGTGCG TGCAATCAAC ATCTACGGAA CCAGTGAGCC GAGCCAGGAG
    TCTGAACTCA CAGCGGTGGG AGAGAAACCT GAGGAGCCCA AGGATGAAGT GGAGGTGTCA
    GATGACGATG AGAAGGAGCC TGAGGTCGAT TACCGGACCG TCACGGTCAA TACGGAACAA
    AAAGTGTCTG ACTTCTACGA CATTGAAGAG AGACTAGGAT CTGGGAAATT TGGACAGGTC
    TTCCGACTTG TAGAGAAGAA AACTGGAAAA ATCTGGGCAG GGAAATTCTT CAAGGCGTAT
    TCAGCAAAAG AGAAAGAGAA CATCCGGCAG GAGATCAGCA TCATGAACTG CCTCCACCAC
    CCCAAGCTGG TCCAGTGTGT GGACGCCTTC GAAGAGAAGG CCAACATCGT CATGGTCCTG
    GAGATCGTGT CCGGGGGCGA GCTGTTCGAG CGCATCATTG ACGAGGACTT TGAGCTGACG
    GAGCGCGAGT GCATCAAGTA CATGCGGCAG ATCTCGGAGG GGGTGGAGTA CATCCACAAG
    CAGGGCATCG TCCACCTGGA CCTCAAGCCT GAGAACATCA TGTGTGTCAA CAAGACGGGC
    ACCCGGATCA AGCTCATCGA CTTCGGTCTG GCCCGGCGGC TGGAGAATGC GGGGTCTCTG
    AAGGTCCTCT TTGGCACCCC GGAGTTTGTG GCGCCTGAAG TGATCAACTA TGAGCCCATT
    GGCTATGCCA CGGACATGTG GAGCATCGGG GTCATCTGCT ACATCCTGGT CAGTGGACTG
    TCCCCCTTTA TGGGTGACAA CGATAACGAG ACCTTAGCCA ACGTTACCTC AGCCACCTGG
    GACTTCGACG ATGAGGCCTT CGATGAGATC TCCGACGACG CCAAGGATTT CATCAGCAAT
    TTACTGAAGA AAGATATGAA AAACCGCTTG GACTGCACCC AGTGCCTCCA GCACCCATGG
    CTAATGAAAG ATACCAAGAA CATGGAGGCC AAGAAGCTCT CCAAGGACCG GATGAAAAAG
    TACATGGCAA GGAGGAAATG GCAGAAAACG GGCAATGCTG TGAGAGCCAT TGGAAGACTG
    TCCTCTATGG CAATGATCTC AGGGCTCAGT GGCAGGAAAT CCTCAACAGG GTCACCAACC
    AGCCCGCTGA ATGCAGAAAA ATTAGAATCT GAAGAAGATG TCTCCCAAGC TTTCCTTGAG
    GCCGTCGCTG AGGAAAAGCC CCATGTAAAA CCCTATTTTT CTAAGACCAT TCGTGATTTA
    GAAGTTGTGG AGGGAAGCGC TGCTAGATTT GACTGCAAGA TTGAAGGATA CCCAGACCCT
    GAGGTTGTCT GGTTCAAAGA TGACCAGTCA ATCAGGGAGT CCCGCCACTT CCAGATAGAC
    TACGATGAGG ATGGGAACTG CTCTTTAATC ATTAGCGATG TCTGCGGTGA TGATGATGCC
    AAGTACACCT GCAAGGCTGT CAACAGTCTT GGAGAAGCCA CCTGCACAGC AGAGCTCATT
    GTGGAAACCA TGGAGGAAGG AGAAGGGGAA GGGGAAGAAG AAGAGGAGGA AGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020925898.1
    CDS509..5845
    Translation

    Target ORF information:

    RefSeq Version XM_021070239.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa myosin light chain kinase (MYLK), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021070239.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGGGCGAT TCTCCTGCAA GATCACTGGC CGGCCCCAAC CGCAGGTCAC CTGGCTCAAG 
    GGAGATGTTC CCCTGCAGCC CAGTGCCCGT GTGACCATGT CTGAGAAGAA TGGGGTGCAA
    GTGCTGGAAA TCCACGAAGT CAACCGAGAC GACGTGGGGG TGTACACGTG CATGGTGGTG
    AATGGGTCGG GGAAGGCCTC CATGTCAGCT GAGCTCTCCA TCCAAGGTTT GGACAATACC
    AATAGGTCGC TTGTGAGAGG AACAAAGGCG GCCAGTTCAG ACATCAGGAC AGAAGCGACC
    AATGGACTCT CACAGGGGCC AAAGCTGGAT GGCCTGGAGG CTGCAGCGGA GAGTAAGAAC
    TGCTCCAGCG CCCAGAGGGG TGGCTCCCCG ACCTGGGCTT CAGGGAGCCA GCCTCAGCCT
    CCGAGGGAAT CCAAGCTGGA GCCGTCAGGG GGCTCAGACA GAAAAGCCCT GAGGAGCCCC
    ATCCTGCAGA AGACTTCCAG TGCCATCACC CTGCAGCCTG CAAAAGTCCA GTTGGAACCA
    AGGGCTCCAG TCTCAGGCAC CCTCTTGCCT TCCAGAGAAG AGAGAGAGCG GCCAGCTGCT
    CCACCTGCCG CCACCCTGCC CTCCAGACAG TCTGGCACAG GAGGCCAAGA AGCTGTGAGC
    AAGGCTGCTA CCAAGCAGGT TGCCATGGAG AGCCGAAGGG CTGCCACAGT CCCCAGATTC
    GAGAGCAAGC CCCAAAGCCA GGAGGTCAGT GAAGATCAGC CAGTCAAGTT CAAGTGTGAA
    GTTTCAGGGG TTCCAAAGCC CGAAGTGACC TGGTTCCTGG ACGGTGCCCC TGTGAAGAGG
    CGAGAAGGCA CCTTTGAGGT TTATGAAGCC GGAGGATCCC ACTACCTCTG CCTGCCAAGA
    GCCCGGGCCA GGGACAGCGG GAACTACAGC TGCACAGCCA CCAACATCCG AGGCCAGGTG
    TCCTGTACCT GGACCCTCCT GGTGAAAAGG CAGCCCATCC AGTACGCCCG CTCCACCTGC
    GAGGCCGGCG TTGCCAAGCT CCACATCCAG GATGCCCTCC CGGAGGACGA CGGCACCTAT
    ACCTGTCTGG CCGAGAACAC CTTGGGGCGG GTGTCCTGCA GCGCCCGGGT CACTGTCCAT
    GAAAAGAAGA GTGATGGGAA GAGTGAGTAC CTCCTGCCCG CTGCCCCCAG CAAGCCCATG
    GCACCCATCT TCCTGCAGGG TCTCTCTGAT CTCAGCGTCA TGGATGGGAG CCAGGTCACC
    ATGACAGTCC AGGTGTCAGG GAACCCACCC CCTGAGGTCA TCTGGCTTCA CGATGGGAAT
    GAGATCCAGG AGTCGGAGGA CTTCCACTTT GAGCAGAGGG GCACTCGGCA CAGCCTCTGT
    ATCCAGGAAG TGTTCCCAGA AGACACAGGC ACATACACCT GTGAAGCCTG GAACAGCGCA
    GGAGAGGTCC GCACCCAGGC CGTGCTCACG GTGCAAGAGC CTCAGGACGG CACCCAGCCT
    TGGTTCATCA GCAAGCCCCG CTCTGTGACA GCCTGCCTGG GCCAGAGTGT CCTCATCTCC
    TGTGCCATAG CTGGGGACCC CTTCCCTACT GTGCACTGGC TCCGAGACGG CAAAGCCCTC
    TCCAAAGACA CTGGCCACTT CGAGGTGCTG CAGAATGAGG ACGTGTTCAC CCTGGTTCTC
    AAGAACGTGC AGCCCTGGCA CGCCGGCCAG TATGAGATCC TGCTCAAGAA CCGGGTTGGC
    GAGTGCAGCT GCCAGGTGTC CCTGGTGCTA CAGAGCAGCC CTGCCAGAGC CCCCCTGCGG
    GGGAGGGAGC CTGCCAGCTG TGAGGGCCAA GGAGCTGGTG CTGATGGTGG TGGTGCTGGC
    AGAGACGGCT ACGGGAGCCT GAGGCCCGGC TGGCCAGCAA GAGGGCAGGG TGAGCCCGAG
    GACGAAGACG GCGAGGACGT GCGCGGGGTG CTCAAGAGGC GCGTGGAGAC GCGGCAGCAC
    ACCGAGGAGG CTATCCGCCA GCAGGAGGTG GAGCAGCTGG ACTTCCGCGA CCTCCTGGGC
    AAGAAGGTGA GCACCAAGAC CTTGTCTGAG GAGGACCTGA AGGAGATCCC TGCCGAGCAG
    ATGGATTTCC GCGCCAATCT GCAGCGGCAA GTCAAGCCGA AGACTGTGTC CGAGGAAGAG
    AGGAAGGTGC ATAGCCCCCA GCAGGTGGAC TTCCGCTCGG TCCTGGCCAA GAAGGGGACG
    CCCAAGACCC CCGTACCTGA GAAGCTGCCC CCTCCAAAAC CTGCCACCCC AGATTTTCGC
    TCGGTGTTGG GCAGTAAGAA AAAATTACCA GCGGAGAATG GTAGCAGCAA TGCTGAGCCT
    TTGAATGTCA AGGCAGCAGA AGGTCCCAAG CCTGTGGGCA ATGCCCAGCC TTCAGGCTTT
    CTCAAACCTA TGGGCAATGC CAAGCTGGCC GAGACCTCCA AACACTTGAG TAATGCCAAG
    CCAGCCGAGC TCCCCAAACC TGTGGGCAAC GCCAAGCCAG CCGAGATCCC CAAACCCGTG
    GGCAACACCA GGCCAGCTGA GACCCCCAAA ACCGTGGGCA ATGCCAAGCC AACTGAGACC
    TTCAAACCCA TGGCCAATAC CAAGCCAGCT GAGACCCTAA AGCCTGTGGG CAATGCCAAG
    CCAGCTGAGA CCCTAAAGCC TGTGGGCAAT GCCAAGCCAG CTGAGACCCT AAAGCCCGTG
    GGCAATGCCA AGCCAGCTGA GACCCCCAAG CCCTTGGGTA ATACCAAGCC AGCCGAGCCC
    CCTAAACCAG CTGGGAAAGA AGAGCTTAAG AAGGAGGTTA AGAATGATGT GAACTACAAG
    AGGGGCTCTG CAGGGGCCAC AGATAATGAA AAAAGCCCGG AGAGCCAAGG GTCAGCCCCA
    ACCTTCAAGG AAAAGCTACG TGATCTCCGT GTGGCAGAGG GTGAGAAGCT GCTGCTCCAG
    TGCCAGGTAT CCTCCGACCC CCCGGCCACC ATCACCTGGA CACTGAATGG GAAGACACTC
    AAGACCACCA AGTTCATTGT CCTCTCCCAA GAAGGCTCAC TGTGCTCCAT CTCCATCGAG
    AAGGCCTTGC CCGAGGACAG AGGTTTGTAC AAGTGTGTGG CCAAGAACAG CGCCGGCCAG
    GCTGAGTGCT CCTGCCAAGT CACCGTGGAC GATGCCCCCA CCGGAGAGAA TGCCAAGGCC
    CCGGAGATGA AAGCCCGGAG GCCAAAGAGC TCTCTTCCTC CTGTGCTAGG AACAGAGAGT
    GATGCCACTG TGAAAAAGAA ACCTGCCCCC AAGACACCTC CGAAGGCAGC CATGCCCCCT
    CAGATCATCC AGTTTCCCGA GGACCAAAAG GTGCGAGCGG GAGAGTCTGT GGAGCTGTTT
    GGTAAAGTGG CCGGTACCCA GCCCATCACC TGTACCTGGA TGAAGTTCCG AAAGCAGATC
    CAGGAGAGTG AGCACATCAA GGTGGAGAAC AGTGAGAATG GCAGCAAGCT CACCATCCGG
    GCCGCGCGCC AGGAGCACTG TGGCTGCTAC ACTCTGCTGG TGGAGAACAA GCTGGGCAGC
    AGGCAGGCCC AGGTCAACCT CACTGTTGTG GACAAGCCGG ACCCCCCCGC AGGCACGCCT
    TGTGCCTCTG ACATCCGCAG CTCCTCGCTG ACCCTGTCCT GGTATGGCTC CTCCTACGAT
    GGGGGCAGCG CCGTACAGTC CTACAGCGTC GAGATCTGGG ACTCAGTGGA CAAGACGTGG
    AAGGAACTAG CCACATGCCG CAGCACCTCT TTTAACGTCC AGGACCTGCT GTCTGACCGG
    GAGTATAAAT TCCGTGTGCG TGCAATCAAC ATCTACGGAA CCAGTGAGCC GAGCCAGGAG
    TCTGAACTCA CAGCGGTGGG AGAGAAACCT GAGGAGCCCA AGGATGAAGT GGAGGTGTCA
    GATGACGATG AGAAGGAGCC TGAGGTCGAT TACCGGACCG TCACGGTCAA TACGGAACAA
    AAAGTGTCTG ACTTCTACGA CATTGAAGAG AGACTAGGAT CTGGGAAATT TGGACAGGTC
    TTCCGACTTG TAGAGAAGAA AACTGGAAAA ATCTGGGCAG GGAAATTCTT CAAGGCGTAT
    TCAGCAAAAG AGAAAGAGAA CATCCGGCAG GAGATCAGCA TCATGAACTG CCTCCACCAC
    CCCAAGCTGG TCCAGTGTGT GGACGCCTTC GAAGAGAAGG CCAACATCGT CATGGTCCTG
    GAGATCGTGT CCGGGGGCGA GCTGTTCGAG CGCATCATTG ACGAGGACTT TGAGCTGACG
    GAGCGCGAGT GCATCAAGTA CATGCGGCAG ATCTCGGAGG GGGTGGAGTA CATCCACAAG
    CAGGGCATCG TCCACCTGGA CCTCAAGCCT GAGAACATCA TGTGTGTCAA CAAGACGGGC
    ACCCGGATCA AGCTCATCGA CTTCGGTCTG GCCCGGCGGC TGGAGAATGC GGGGTCTCTG
    AAGGTCCTCT TTGGCACCCC GGAGTTTGTG GCGCCTGAAG TGATCAACTA TGAGCCCATT
    GGCTATGCCA CGGACATGTG GAGCATCGGG GTCATCTGCT ACATCCTGGT CAGTGGACTG
    TCCCCCTTTA TGGGTGACAA CGATAACGAG ACCTTAGCCA ACGTTACCTC AGCCACCTGG
    GACTTCGACG ATGAGGCCTT CGATGAGATC TCCGACGACG CCAAGGATTT CATCAGCAAT
    TTACTGAAGA AAGATATGAA AAACCGCTTG GACTGCACCC AGTGCCTCCA GCACCCATGG
    CTAATGAAAG ATACCAAGAA CATGGAGGCC AAGAAGCTCT CCAAGGACCG GATGAAAAAG
    TACATGGCAA GGAGGAAATG GCAGAAAACG GGCAATGCTG TGAGAGCCAT TGGAAGACTG
    TCCTCTATGG CAATGATCTC AGGGCTCAGT GGCAGGAAAT CCTCAACAGG GTCACCAACC
    AGCCCGCTGA ATGCAGAAAA ATTAGAATCT GAAGAAGATG TCTCCCAAGC TTTCCTTGAG
    GCCGTCGCTG AGGAAAAGCC CCATGTAAAA CCCTATTTTT CTAAGACCAT TCGTGATTTA
    GAAGTTGTGG AGGGAAGCGC TGCTAGATTT GACTGCAAGA TTGAAGGATA CCCAGACCCT
    GAGGTTGTCT GGTTCAAAGA TGACCAGTCA ATCAGGGAGT CCCGCCACTT CCAGATAGAC
    TACGATGAGG ATGGGAACTG CTCTTTAATC ATTAGCGATG TCTGCGGTGA TGATGATGCC
    AAGTACACCT GCAAGGCTGT CAACAGTCTT GGAGAAGCCA CCTGCACAGC AGAGCTCATT
    GTGGAAACCA TGGAGGAAGG AGAAGGGGAA GGGGAAGAAG AAGAGGAGGA AGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe236099
    Clone ID Related Accession (Same CDS sequence) XM_021070240.1
    Accession Version XM_021070240.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5334bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product myosin light chain kinase, smooth muscle isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010455.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGGGCGAT TCTCCTGCAA GATCACTGGC CGGCCCCAAC CGCAGGTCAC CTGGCTCAAG 
    GGAGATGTTC CCCTGCAGCC CAGTGCCCGT GTGACCATGT CTGAGAAGAA TGGGGTGCAA
    GTGCTGGAAA TCCACGAAGT CAACCGAGAC GACGTGGGGG TGTACACGTG CATGGTGGTG
    AATGGGTCGG GGAAGGCCTC CATGTCAGCT GAGCTCTCCA TCCAAGGTTT GGACAATACC
    AATAGGTCGC TTGTGAGAGG AACAAAGGCG GCCAGTTCAG ACATCAGGAC AGAAGCGACC
    AATGGACTCT CACAGGGGCC AAAGCTGGAT GGCCTGGAGG CTGCAGCGGA GAGTAAGAAC
    TGCTCCAGCG CCCAGAGGGG TGGCTCCCCG ACCTGGGCTT CAGGGAGCCA GCCTCAGCCT
    CCGAGGGAAT CCAAGCTGGA GCCGTCAGGG GGCTCAGACA GAAAAGCCCT GAGGAGCCCC
    ATCCTGCAGA AGACTTCCAG TGCCATCACC CTGCAGCCTG CAAAAGTCCA GTTGGAACCA
    AGGGCTCCAG TCTCAGGCAC CCTCTTGCCT TCCAGAGAAG AGAGAGAGCG GCCAGCTGCT
    CCACCTGCCG CCACCCTGCC CTCCAGACAG TCTGGCACAG GAGGCCAAGA AGCTGTGAGC
    AAGGCTGCTA CCAAGCAGGT TGCCATGGAG AGCCGAAGGG CTGCCACAGT CCCCAGATTC
    GAGAGCAAGC CCCAAAGCCA GGAGGTCAGT GAAGATCAGC CAGTCAAGTT CAAGTGTGAA
    GTTTCAGGGG TTCCAAAGCC CGAAGTGACC TGGTTCCTGG ACGGTGCCCC TGTGAAGAGG
    CGAGAAGGCA CCTTTGAGGT TTATGAAGCC GGAGGATCCC ACTACCTCTG CCTGCCAAGA
    GCCCGGGCCA GGGACAGCGG GAACTACAGC TGCACAGCCA CCAACATCCG AGGCCAGGTG
    TCCTGTACCT GGACCCTCCT GGTGAAAAGG CAGCCCATCC AGTACGCCCG CTCCACCTGC
    GAGGCCGGCG TTGCCAAGCT CCACATCCAG GATGCCCTCC CGGAGGACGA CGGCACCTAT
    ACCTGTCTGG CCGAGAACAC CTTGGGGCGG GTGTCCTGCA GCGCCCGGGT CACTGTCCAT
    GAAAAGAAGA GTGATGGGAA GAGTGAGTAC CTCCTGCCCG CTGCCCCCAG CAAGCCCATG
    GCACCCATCT TCCTGCAGGG TCTCTCTGAT CTCAGCGTCA TGGATGGGAG CCAGGTCACC
    ATGACAGTCC AGGTGTCAGG GAACCCACCC CCTGAGGTCA TCTGGCTTCA CGATGGGAAT
    GAGATCCAGG AGTCGGAGGA CTTCCACTTT GAGCAGAGGG GCACTCGGCA CAGCCTCTGT
    ATCCAGGAAG TGTTCCCAGA AGACACAGGC ACATACACCT GTGAAGCCTG GAACAGCGCA
    GGAGAGGTCC GCACCCAGGC CGTGCTCACG GTGCAAGAGC CTCAGGACGG CACCCAGCCT
    TGGTTCATCA GCAAGCCCCG CTCTGTGACA GCCTGCCTGG GCCAGAGTGT CCTCATCTCC
    TGTGCCATAG CTGGGGACCC CTTCCCTACT GTGCACTGGC TCCGAGACGG CAAAGCCCTC
    TCCAAAGACA CTGGCCACTT CGAGGTGCTG CAGAATGAGG ACGTGTTCAC CCTGGTTCTC
    AAGAACGTGC AGCCCTGGCA CGCCGGCCAG TATGAGATCC TGCTCAAGAA CCGGGTTGGC
    GAGTGCAGCT GCCAGGTGTC CCTGGTGCTA CAGAGCAGCC CTGCCAGAGC CCCCCTGCGG
    GGGAGGGAGC CTGCCAGCTG TGAGGGCCAA GGAGCTGGTG CTGATGGTGG TGGTGCTGGC
    AGAGACGGCT ACGGGAGCCT GAGGCCCGGC TGGCCAGCAA GAGGGCAGGG TGAGCCCGAG
    GACGAAGACG GCGAGGACGT GCGCGGGGTG CTCAAGAGGC GCGTGGAGAC GCGGCAGCAC
    ACCGAGGAGG CTATCCGCCA GCAGGAGGTG GAGCAGCTGG ACTTCCGCGA CCTCCTGGGC
    AAGAAGGTGA GCACCAAGAC CTTGTCTGAG GAGGACCTGA AGGAGATCCC TGCCGAGCAG
    ATGGATTTCC GCGCCAATCT GCAGCGGCAA GTCAAGCCGA AGACTGTGTC CGAGGAAGAG
    AGGAAGGTGC ATAGCCCCCA GCAGGTGGAC TTCCGCTCGG TCCTGGCCAA GAAGGGGACG
    CCCAAGACCC CCGTACCTGA GAAGCTGCCC CCTCCAAAAC CTGCCACCCC AGATTTTCGC
    TCGGTGTTGG GCAGTAAGAA AAAATTACCA GCGGAGAATG GTAGCAGCAA TGCTGAGCCT
    TTGAATGTCA AGGCAGCAGA AGGTCCCAAG CCTGTGGGCA ATGCCCAGCC TTCAGGCTTT
    CTCAAACCTA TGGGCAATGC CAAGCTGGCC GAGACCTCCA AACACTTGAG TAATGCCAAG
    CCAGCCGAGC TCCCCAAACC TGTGGGCAAC GCCAAGCCAG CCGAGATCCC CAAACCCGTG
    GGCAACACCA GGCCAGCTGA GACCCCCAAA ACCGTGGGCA ATGCCAAGCC AACTGAGACC
    TTCAAACCCA TGGCCAATAC CAAGCCAGCT GAGACCCTAA AGCCTGTGGG CAATGCCAAG
    CCAGCTGAGA CCCTAAAGCC TGTGGGCAAT GCCAAGCCAG CTGAGACCCT AAAGCCCGTG
    GGCAATGCCA AGCCAGCTGA GACCCCCAAG CCCTTGGGTA ATACCAAGCC AGCCGAGCCC
    CCTAAACCAG CTGGGAAAGA AGAGCTTAAG AAGGAGGTTA AGAATGATGT GAACTACAAG
    AGGGGCTCTG CAGGGGCCAC AGATAATGAA AAAAGCCCGG AGAGCCAAGG GTCAGCCCCA
    ACCTTCAAGG AAAAGCTACG TGATCTCCGT GTGGCAGAGG GTGAGAAGCT GCTGCTCCAG
    TGCCAGGTAT CCTCCGACCC CCCGGCCACC ATCACCTGGA CACTGAATGG GAAGACACTC
    AAGACCACCA AGTTCATTGT CCTCTCCCAA GAAGGCTCAC TGTGCTCCAT CTCCATCGAG
    AAGGCCTTGC CCGAGGACAG AGGTTTGTAC AAGTGTGTGG CCAAGAACAG CGCCGGCCAG
    GCTGAGTGCT CCTGCCAAGT CACCGTGGAC GATGCCCCCA CCGGAGAGAA TGCCAAGGCC
    CCGGAGATGA AAGCCCGGAG GCCAAAGAGC TCTCTTCCTC CTGTGCTAGG AACAGAGAGT
    GATGCCACTG TGAAAAAGAA ACCTGCCCCC AAGACACCTC CGAAGGCAGC CATGCCCCCT
    CAGATCATCC AGTTTCCCGA GGACCAAAAG GTGCGAGCGG GAGAGTCTGT GGAGCTGTTT
    GGTAAAGTGG CCGGTACCCA GCCCATCACC TGTACCTGGA TGAAGTTCCG AAAGCAGATC
    CAGGAGAGTG AGCACATCAA GGTGGAGAAC AGTGAGAATG GCAGCAAGCT CACCATCCGG
    GCCGCGCGCC AGGAGCACTG TGGCTGCTAC ACTCTGCTGG TGGAGAACAA GCTGGGCAGC
    AGGCAGGCCC AGGTCAACCT CACTGTTGTG GACAAGCCGG ACCCCCCCGC AGGCACGCCT
    TGTGCCTCTG ACATCCGCAG CTCCTCGCTG ACCCTGTCCT GGTATGGCTC CTCCTACGAT
    GGGGGCAGCG CCGTACAGTC CTACAGCGTC GAGATCTGGG ACTCAGTGGA CAAGACGTGG
    AAGGAACTAG CCACATGCCG CAGCACCTCT TTTAACGTCC AGGACCTGCT GTCTGACCGG
    GAGTATAAAT TCCGTGTGCG TGCAATCAAC ATCTACGGAA CCAGTGAGCC GAGCCAGGAG
    TCTGAACTCA CAGCGGTGGG AGAGAAACCT GAGGAGCCCA AGGATGAAGT GGAGGTGTCA
    GATGACGATG AGAAGGAGCC TGAGGTCGAT TACCGGACCG TCACGGTCAA TACGGAACAA
    AAAGTGTCTG ACTTCTACGA CATTGAAGAG AGACTAGGAT CTGGGAAATT TGGACAGGTC
    TTCCGACTTG TAGAGAAGAA AACTGGAAAA ATCTGGGCAG GGAAATTCTT CAAGGCGTAT
    TCAGCAAAAG AGAAAGAGAA CATCCGGCAG GAGATCAGCA TCATGAACTG CCTCCACCAC
    CCCAAGCTGG TCCAGTGTGT GGACGCCTTC GAAGAGAAGG CCAACATCGT CATGGTCCTG
    GAGATCGTGT CCGGGGGCGA GCTGTTCGAG CGCATCATTG ACGAGGACTT TGAGCTGACG
    GAGCGCGAGT GCATCAAGTA CATGCGGCAG ATCTCGGAGG GGGTGGAGTA CATCCACAAG
    CAGGGCATCG TCCACCTGGA CCTCAAGCCT GAGAACATCA TGTGTGTCAA CAAGACGGGC
    ACCCGGATCA AGCTCATCGA CTTCGGTCTG GCCCGGCGGC TGGAGAATGC GGGGTCTCTG
    AAGGTCCTCT TTGGCACCCC GGAGTTTGTG GCGCCTGAAG TGATCAACTA TGAGCCCATT
    GGCTATGCCA CGGACATGTG GAGCATCGGG GTCATCTGCT ACATCCTGGT CAGTGGACTG
    TCCCCCTTTA TGGGTGACAA CGATAACGAG ACCTTAGCCA ACGTTACCTC AGCCACCTGG
    GACTTCGACG ATGAGGCCTT CGATGAGATC TCCGACGACG CCAAGGATTT CATCAGCAAT
    TTACTGAAGA AAGATATGAA AAACCGCTTG GACTGCACCC AGTGCCTCCA GCACCCATGG
    CTAATGAAAG ATACCAAGAA CATGGAGGCC AAGAAGCTCT CCAAGGACCG GATGAAAAAG
    TACATGGCAA GGAGGAAATG GCAGAAAACG GGCAATGCTG TGAGAGCCAT TGGAAGACTG
    TCCTCTATGG CAATGATCTC AGGGCTCAGT GGCAGGAAAT CCTCAACAGG GTCACCAACC
    AGCCCGCTGA ATGCAGAAAA ATTAGAATCT GAAGATGTCT CCCAAGCTTT CCTTGAGGCC
    GTCGCTGAGG AAAAGCCCCA TGTAAAACCC TATTTTTCTA AGACCATTCG TGATTTAGAA
    GTTGTGGAGG GAAGCGCTGC TAGATTTGAC TGCAAGATTG AAGGATACCC AGACCCTGAG
    GTTGTCTGGT TCAAAGATGA CCAGTCAATC AGGGAGTCCC GCCACTTCCA GATAGACTAC
    GATGAGGATG GGAACTGCTC TTTAATCATT AGCGATGTCT GCGGTGATGA TGATGCCAAG
    TACACCTGCA AGGCTGTCAA CAGTCTTGGA GAAGCCACCT GCACAGCAGA GCTCATTGTG
    GAAACCATGG AGGAAGGAGA AGGGGAAGGG GAAGAAGAAG AGGAGGAAGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020925899.1
    CDS511..5844
    Translation

    Target ORF information:

    RefSeq Version XM_021070240.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa myosin light chain kinase (MYLK), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021070240.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGGGCGAT TCTCCTGCAA GATCACTGGC CGGCCCCAAC CGCAGGTCAC CTGGCTCAAG 
    GGAGATGTTC CCCTGCAGCC CAGTGCCCGT GTGACCATGT CTGAGAAGAA TGGGGTGCAA
    GTGCTGGAAA TCCACGAAGT CAACCGAGAC GACGTGGGGG TGTACACGTG CATGGTGGTG
    AATGGGTCGG GGAAGGCCTC CATGTCAGCT GAGCTCTCCA TCCAAGGTTT GGACAATACC
    AATAGGTCGC TTGTGAGAGG AACAAAGGCG GCCAGTTCAG ACATCAGGAC AGAAGCGACC
    AATGGACTCT CACAGGGGCC AAAGCTGGAT GGCCTGGAGG CTGCAGCGGA GAGTAAGAAC
    TGCTCCAGCG CCCAGAGGGG TGGCTCCCCG ACCTGGGCTT CAGGGAGCCA GCCTCAGCCT
    CCGAGGGAAT CCAAGCTGGA GCCGTCAGGG GGCTCAGACA GAAAAGCCCT GAGGAGCCCC
    ATCCTGCAGA AGACTTCCAG TGCCATCACC CTGCAGCCTG CAAAAGTCCA GTTGGAACCA
    AGGGCTCCAG TCTCAGGCAC CCTCTTGCCT TCCAGAGAAG AGAGAGAGCG GCCAGCTGCT
    CCACCTGCCG CCACCCTGCC CTCCAGACAG TCTGGCACAG GAGGCCAAGA AGCTGTGAGC
    AAGGCTGCTA CCAAGCAGGT TGCCATGGAG AGCCGAAGGG CTGCCACAGT CCCCAGATTC
    GAGAGCAAGC CCCAAAGCCA GGAGGTCAGT GAAGATCAGC CAGTCAAGTT CAAGTGTGAA
    GTTTCAGGGG TTCCAAAGCC CGAAGTGACC TGGTTCCTGG ACGGTGCCCC TGTGAAGAGG
    CGAGAAGGCA CCTTTGAGGT TTATGAAGCC GGAGGATCCC ACTACCTCTG CCTGCCAAGA
    GCCCGGGCCA GGGACAGCGG GAACTACAGC TGCACAGCCA CCAACATCCG AGGCCAGGTG
    TCCTGTACCT GGACCCTCCT GGTGAAAAGG CAGCCCATCC AGTACGCCCG CTCCACCTGC
    GAGGCCGGCG TTGCCAAGCT CCACATCCAG GATGCCCTCC CGGAGGACGA CGGCACCTAT
    ACCTGTCTGG CCGAGAACAC CTTGGGGCGG GTGTCCTGCA GCGCCCGGGT CACTGTCCAT
    GAAAAGAAGA GTGATGGGAA GAGTGAGTAC CTCCTGCCCG CTGCCCCCAG CAAGCCCATG
    GCACCCATCT TCCTGCAGGG TCTCTCTGAT CTCAGCGTCA TGGATGGGAG CCAGGTCACC
    ATGACAGTCC AGGTGTCAGG GAACCCACCC CCTGAGGTCA TCTGGCTTCA CGATGGGAAT
    GAGATCCAGG AGTCGGAGGA CTTCCACTTT GAGCAGAGGG GCACTCGGCA CAGCCTCTGT
    ATCCAGGAAG TGTTCCCAGA AGACACAGGC ACATACACCT GTGAAGCCTG GAACAGCGCA
    GGAGAGGTCC GCACCCAGGC CGTGCTCACG GTGCAAGAGC CTCAGGACGG CACCCAGCCT
    TGGTTCATCA GCAAGCCCCG CTCTGTGACA GCCTGCCTGG GCCAGAGTGT CCTCATCTCC
    TGTGCCATAG CTGGGGACCC CTTCCCTACT GTGCACTGGC TCCGAGACGG CAAAGCCCTC
    TCCAAAGACA CTGGCCACTT CGAGGTGCTG CAGAATGAGG ACGTGTTCAC CCTGGTTCTC
    AAGAACGTGC AGCCCTGGCA CGCCGGCCAG TATGAGATCC TGCTCAAGAA CCGGGTTGGC
    GAGTGCAGCT GCCAGGTGTC CCTGGTGCTA CAGAGCAGCC CTGCCAGAGC CCCCCTGCGG
    GGGAGGGAGC CTGCCAGCTG TGAGGGCCAA GGAGCTGGTG CTGATGGTGG TGGTGCTGGC
    AGAGACGGCT ACGGGAGCCT GAGGCCCGGC TGGCCAGCAA GAGGGCAGGG TGAGCCCGAG
    GACGAAGACG GCGAGGACGT GCGCGGGGTG CTCAAGAGGC GCGTGGAGAC GCGGCAGCAC
    ACCGAGGAGG CTATCCGCCA GCAGGAGGTG GAGCAGCTGG ACTTCCGCGA CCTCCTGGGC
    AAGAAGGTGA GCACCAAGAC CTTGTCTGAG GAGGACCTGA AGGAGATCCC TGCCGAGCAG
    ATGGATTTCC GCGCCAATCT GCAGCGGCAA GTCAAGCCGA AGACTGTGTC CGAGGAAGAG
    AGGAAGGTGC ATAGCCCCCA GCAGGTGGAC TTCCGCTCGG TCCTGGCCAA GAAGGGGACG
    CCCAAGACCC CCGTACCTGA GAAGCTGCCC CCTCCAAAAC CTGCCACCCC AGATTTTCGC
    TCGGTGTTGG GCAGTAAGAA AAAATTACCA GCGGAGAATG GTAGCAGCAA TGCTGAGCCT
    TTGAATGTCA AGGCAGCAGA AGGTCCCAAG CCTGTGGGCA ATGCCCAGCC TTCAGGCTTT
    CTCAAACCTA TGGGCAATGC CAAGCTGGCC GAGACCTCCA AACACTTGAG TAATGCCAAG
    CCAGCCGAGC TCCCCAAACC TGTGGGCAAC GCCAAGCCAG CCGAGATCCC CAAACCCGTG
    GGCAACACCA GGCCAGCTGA GACCCCCAAA ACCGTGGGCA ATGCCAAGCC AACTGAGACC
    TTCAAACCCA TGGCCAATAC CAAGCCAGCT GAGACCCTAA AGCCTGTGGG CAATGCCAAG
    CCAGCTGAGA CCCTAAAGCC TGTGGGCAAT GCCAAGCCAG CTGAGACCCT AAAGCCCGTG
    GGCAATGCCA AGCCAGCTGA GACCCCCAAG CCCTTGGGTA ATACCAAGCC AGCCGAGCCC
    CCTAAACCAG CTGGGAAAGA AGAGCTTAAG AAGGAGGTTA AGAATGATGT GAACTACAAG
    AGGGGCTCTG CAGGGGCCAC AGATAATGAA AAAAGCCCGG AGAGCCAAGG GTCAGCCCCA
    ACCTTCAAGG AAAAGCTACG TGATCTCCGT GTGGCAGAGG GTGAGAAGCT GCTGCTCCAG
    TGCCAGGTAT CCTCCGACCC CCCGGCCACC ATCACCTGGA CACTGAATGG GAAGACACTC
    AAGACCACCA AGTTCATTGT CCTCTCCCAA GAAGGCTCAC TGTGCTCCAT CTCCATCGAG
    AAGGCCTTGC CCGAGGACAG AGGTTTGTAC AAGTGTGTGG CCAAGAACAG CGCCGGCCAG
    GCTGAGTGCT CCTGCCAAGT CACCGTGGAC GATGCCCCCA CCGGAGAGAA TGCCAAGGCC
    CCGGAGATGA AAGCCCGGAG GCCAAAGAGC TCTCTTCCTC CTGTGCTAGG AACAGAGAGT
    GATGCCACTG TGAAAAAGAA ACCTGCCCCC AAGACACCTC CGAAGGCAGC CATGCCCCCT
    CAGATCATCC AGTTTCCCGA GGACCAAAAG GTGCGAGCGG GAGAGTCTGT GGAGCTGTTT
    GGTAAAGTGG CCGGTACCCA GCCCATCACC TGTACCTGGA TGAAGTTCCG AAAGCAGATC
    CAGGAGAGTG AGCACATCAA GGTGGAGAAC AGTGAGAATG GCAGCAAGCT CACCATCCGG
    GCCGCGCGCC AGGAGCACTG TGGCTGCTAC ACTCTGCTGG TGGAGAACAA GCTGGGCAGC
    AGGCAGGCCC AGGTCAACCT CACTGTTGTG GACAAGCCGG ACCCCCCCGC AGGCACGCCT
    TGTGCCTCTG ACATCCGCAG CTCCTCGCTG ACCCTGTCCT GGTATGGCTC CTCCTACGAT
    GGGGGCAGCG CCGTACAGTC CTACAGCGTC GAGATCTGGG ACTCAGTGGA CAAGACGTGG
    AAGGAACTAG CCACATGCCG CAGCACCTCT TTTAACGTCC AGGACCTGCT GTCTGACCGG
    GAGTATAAAT TCCGTGTGCG TGCAATCAAC ATCTACGGAA CCAGTGAGCC GAGCCAGGAG
    TCTGAACTCA CAGCGGTGGG AGAGAAACCT GAGGAGCCCA AGGATGAAGT GGAGGTGTCA
    GATGACGATG AGAAGGAGCC TGAGGTCGAT TACCGGACCG TCACGGTCAA TACGGAACAA
    AAAGTGTCTG ACTTCTACGA CATTGAAGAG AGACTAGGAT CTGGGAAATT TGGACAGGTC
    TTCCGACTTG TAGAGAAGAA AACTGGAAAA ATCTGGGCAG GGAAATTCTT CAAGGCGTAT
    TCAGCAAAAG AGAAAGAGAA CATCCGGCAG GAGATCAGCA TCATGAACTG CCTCCACCAC
    CCCAAGCTGG TCCAGTGTGT GGACGCCTTC GAAGAGAAGG CCAACATCGT CATGGTCCTG
    GAGATCGTGT CCGGGGGCGA GCTGTTCGAG CGCATCATTG ACGAGGACTT TGAGCTGACG
    GAGCGCGAGT GCATCAAGTA CATGCGGCAG ATCTCGGAGG GGGTGGAGTA CATCCACAAG
    CAGGGCATCG TCCACCTGGA CCTCAAGCCT GAGAACATCA TGTGTGTCAA CAAGACGGGC
    ACCCGGATCA AGCTCATCGA CTTCGGTCTG GCCCGGCGGC TGGAGAATGC GGGGTCTCTG
    AAGGTCCTCT TTGGCACCCC GGAGTTTGTG GCGCCTGAAG TGATCAACTA TGAGCCCATT
    GGCTATGCCA CGGACATGTG GAGCATCGGG GTCATCTGCT ACATCCTGGT CAGTGGACTG
    TCCCCCTTTA TGGGTGACAA CGATAACGAG ACCTTAGCCA ACGTTACCTC AGCCACCTGG
    GACTTCGACG ATGAGGCCTT CGATGAGATC TCCGACGACG CCAAGGATTT CATCAGCAAT
    TTACTGAAGA AAGATATGAA AAACCGCTTG GACTGCACCC AGTGCCTCCA GCACCCATGG
    CTAATGAAAG ATACCAAGAA CATGGAGGCC AAGAAGCTCT CCAAGGACCG GATGAAAAAG
    TACATGGCAA GGAGGAAATG GCAGAAAACG GGCAATGCTG TGAGAGCCAT TGGAAGACTG
    TCCTCTATGG CAATGATCTC AGGGCTCAGT GGCAGGAAAT CCTCAACAGG GTCACCAACC
    AGCCCGCTGA ATGCAGAAAA ATTAGAATCT GAAGATGTCT CCCAAGCTTT CCTTGAGGCC
    GTCGCTGAGG AAAAGCCCCA TGTAAAACCC TATTTTTCTA AGACCATTCG TGATTTAGAA
    GTTGTGGAGG GAAGCGCTGC TAGATTTGAC TGCAAGATTG AAGGATACCC AGACCCTGAG
    GTTGTCTGGT TCAAAGATGA CCAGTCAATC AGGGAGTCCC GCCACTTCCA GATAGACTAC
    GATGAGGATG GGAACTGCTC TTTAATCATT AGCGATGTCT GCGGTGATGA TGATGCCAAG
    TACACCTGCA AGGCTGTCAA CAGTCTTGGA GAAGCCACCT GCACAGCAGA GCTCATTGTG
    GAAACCATGG AGGAAGGAGA AGGGGAAGGG GAAGAAGAAG AGGAGGAAGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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