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GOT1 cDNA ORF clone, Sus scrofa(pig)

Summary

Gene Symbol GOT1
Entrez Gene ID 396967
Full Name glutamic-oxaloacetic transaminase 1
Synonyms CASPAT,cCAT
Gene Type protein-coding
Organism Sus scrofa(pig)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_213927.1 NP_999092.1 aspartate aminotransferase, cytoplasmic

Pathways from BioSystems


Gene Ontology


Bibliography

Related articles in PubMed

SNPs in the porcine GOT1 gene improve a QTL for serum aspartate aminotransferase activity on SSC14.
Reiner G, Clemens N, Lohner E, Willems H
Animal genetics41(3)319-23(2010 Jun)

PEDE (Pig EST Data Explorer): construction of a database for ESTs derived from porcine full-length cDNA libraries.
Uenishi H, Eguchi T, Suzuki K, Sawazaki T, Toki D, Shinkai H, Okumura N, Hamasima N, Awata T
Nucleic acids research32(Database issue)D484-8(2004 Jan)

Refinement and comparisons of the crystal structures of pig cytosolic aspartate aminotransferase and its complex with 2-methylaspartate.
Rhee S, Silva MM, Hyde CC, Rogers PH, Metzler CM, Metzler DE, Arnone A
The Journal of biological chemistry272(28)17293-302(1997 Jul)

The complete amino acid sequence of cytoplasmic aspartate aminotransferase from pig heart.
Ovchinnikov YA, Egorov CA, Aldanova NA, Feigina MY, Lipkin VM, Abdulaev NG, Grishin EV, Kiselev AP, Modyanov NN, Braunstein AE, Polyanovsky OL, Nosikov VV
FEBS letters29(1)31-34(1973 Jan)

Primary structure of pyridoxal phosphate binding site in the mitochondrial and extramitochondrial aspartate aminotransferases from pig heart muscle. Chymotryptic peptides.
Morino Y, Watanabe T
Biochemistry8(8)3412-7(1969 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

GOT1 is a suitable candidate gene for the serum aspartate aminotransferase activity QTL on SSC14.
Title: SNPs in the porcine GOT1 gene improve a QTL for serum aspartate aminotransferase activity on SSC14.

The following GOT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GOT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSe30400 NM_213927.1
Latest version!		 Sus scrofa glutamic-oxaloacetic transaminase 1 (GOT1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OSe30400
Clone ID Related Accession (Same CDS sequence) NM_213927.1
Accession Version NM_213927.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1242bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-24
Organism Sus scrofa(pig)
Product aspartate aminotransferase, cytoplasmic
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M24088.1. ##Evidence-Data-START## Transcript exon combination :: M24088.1, AK234797.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02584787, SAMN02584789 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGCACCTC CATCAGTCTT TGCCGAGGTT CCTCAGGCCC AGCCGGTCCT TGTCTTTAAG 
CTCATTGCTG ACTTCCGGGA GGATCCGGAC CCCCGCAAGG TCAACCTAGG AGTGGGAGCT 
TATCGCACCG ATGATTGCCA GCCTTGGGTT TTGCCAGTCG TGAGGAAGGT GGAGCAGAGG 
ATTGCTAATG ACAGCAGCCT AAACCACGAG TACCTGCCCA TCCTGGGCCT GGCAGAGTTC 
CGGACCTGTG CTTCCCGCCT TGCCCTTGGA GATGACAGCC CAGCTCTTCA GGAGAAGCGG 
GTGGGAGGGG TGCAGTCTTT GGGGGGAACG GGTGCACTTC GAATTGGAGC TGAGTTCTTA 
GCACGATGGT ACAATGGAAC GAACAACAAA GACACGCCTG TCTACGTATC CTCACCAACC 
TGGGAAAATC ACAATGGAGT CTTCACTACT GCTGGATTCA AAGACATTCG GTCCTATCGC 
TATTGGGATA CCGAGAAGAG AGGACTTGAT CTCCAGGGTT TCCTGAGTGA TCTGGAGAAC 
GCTCCTGAGT TCTCCATCTT TGTCCTCCAC GCCTGTGCCC ACAACCCGAC AGGGACCGAC 
CCAACTCCGG AGCAATGGAA GCAGATCGCC TCTGTCATGA AGCGCCGGTT TCTGTTCCCC 
TTCTTTGACT CAGCCTATCA GGGCTTCGCA TCTGGCAACC TAGAAAAAGA CGCCTGGGCC 
ATTCGCTATT TTGTGTCTGA AGGGTTCGAG CTCTTCTGTG CCCAGTCCTT CTCCAAGAAC 
TTCGGGCTCT ACAATGAGCG CGTGGGGAAC CTGACCGTGG TTGCAAAAGA ACCCGATAGC 
ATCCTGCGAG TCCTTTCCCA GATGGAGAAG ATCGTGCGAG TGACGTGGTC CAATCCCCCT 
GCTCAGGGAG CGAGAATCGT GGCCCGTACG CTCTCTGACC CTGAGCTCTT TCATGAATGG 
ACAGGTAACG TGAAGACAAT GGCTGACCGC ATTCTGAGCA TGAGATCTGA GCTTAGGGCA 
CGATTAGAAG CCCTCAAGAC CCCTGGAACC TGGAACCACA TCACGGACCA GATTGGAATG 
TTCAGCTTCA CTGGGTTGAA CCCCAAGCAG GTTGAATATC TGATCAACGA AAAGCACATC 
TATCTGCTAC CAAGTGGTCG GATCAACATG TGTGGCTTAA CCACCAAAAA TCTAGATTAT 
GTGGCCACCT CCATCCATGA AGCTGTCACC AAAATCCAGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_999092.1
CDS54..1295
Translation

Target ORF information:

RefSeq Version NM_213927.1
Organism Sus scrofa(pig)
Definition Sus scrofa glutamic-oxaloacetic transaminase 1 (GOT1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_213927.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGCACCTC CATCAGTCTT TGCCGAGGTT CCTCAGGCCC AGCCGGTCCT TGTCTTTAAG 
CTCATTGCTG ACTTCCGGGA GGATCCGGAC CCCCGCAAGG TCAACCTAGG AGTGGGAGCT 
TATCGCACCG ATGATTGCCA GCCTTGGGTT TTGCCAGTCG TGAGGAAGGT GGAGCAGAGG 
ATTGCTAATG ACAGCAGCCT AAACCACGAG TACCTGCCCA TCCTGGGCCT GGCAGAGTTC 
CGGACCTGTG CTTCCCGCCT TGCCCTTGGA GATGACAGCC CAGCTCTTCA GGAGAAGCGG 
GTGGGAGGGG TGCAGTCTTT GGGGGGAACG GGTGCACTTC GAATTGGAGC TGAGTTCTTA 
GCACGATGGT ACAATGGAAC GAACAACAAA GACACGCCTG TCTACGTATC CTCACCAACC 
TGGGAAAATC ACAATGGAGT CTTCACTACT GCTGGATTCA AAGACATTCG GTCCTATCGC 
TATTGGGATA CCGAGAAGAG AGGACTTGAT CTCCAGGGTT TCCTGAGTGA TCTGGAGAAC 
GCTCCTGAGT TCTCCATCTT TGTCCTCCAC GCCTGTGCCC ACAACCCGAC AGGGACCGAC 
CCAACTCCGG AGCAATGGAA GCAGATCGCC TCTGTCATGA AGCGCCGGTT TCTGTTCCCC 
TTCTTTGACT CAGCCTATCA GGGCTTCGCA TCTGGCAACC TAGAAAAAGA CGCCTGGGCC 
ATTCGCTATT TTGTGTCTGA AGGGTTCGAG CTCTTCTGTG CCCAGTCCTT CTCCAAGAAC 
TTCGGGCTCT ACAATGAGCG CGTGGGGAAC CTGACCGTGG TTGCAAAAGA ACCCGATAGC 
ATCCTGCGAG TCCTTTCCCA GATGGAGAAG ATCGTGCGAG TGACGTGGTC CAATCCCCCT 
GCTCAGGGAG CGAGAATCGT GGCCCGTACG CTCTCTGACC CTGAGCTCTT TCATGAATGG 
ACAGGTAACG TGAAGACAAT GGCTGACCGC ATTCTGAGCA TGAGATCTGA GCTTAGGGCA 
CGATTAGAAG CCCTCAAGAC CCCTGGAACC TGGAACCACA TCACGGACCA GATTGGAATG 
TTCAGCTTCA CTGGGTTGAA CCCCAAGCAG GTTGAATATC TGATCAACGA AAAGCACATC 
TATCTGCTAC CAAGTGGTCG GATCAACATG TGTGGCTTAA CCACCAAAAA TCTAGATTAT 
GTGGCCACCT CCATCCATGA AGCTGTCACC AAAATCCAGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

SNPs in the porcine GOT1 gene improve a QTL for serum aspartate aminotransferase activity on SSC14.
Animal genetics41(3)319-23(2010 Jun)
Reiner G,Clemens N,Lohner E,Willems H


book

PEDE (Pig EST Data Explorer): construction of a database for ESTs derived from porcine full-length cDNA libraries.
Nucleic acids research32(Database issue)D484-8(2004 Jan)
Uenishi H,Eguchi T,Suzuki K,Sawazaki T,Toki D,Shinkai H,Okumura N,Hamasima N,Awata T


book

Refinement and comparisons of the crystal structures of pig cytosolic aspartate aminotransferase and its complex with 2-methylaspartate.
The Journal of biological chemistry272(28)17293-302(1997 Jul)
Rhee S,Silva MM,Hyde CC,Rogers PH,Metzler CM,Metzler DE,Arnone A


book

The complete amino acid sequence of cytoplasmic aspartate aminotransferase from pig heart.
FEBS letters29(1)31-34(1973 Jan)
Ovchinnikov YA,Egorov CA,Aldanova NA,Feigina MY,Lipkin VM,Abdulaev NG,Grishin EV,Kiselev AP,Modyanov NN,Braunstein AE,Polyanovsky OL,Nosikov VV


book

Primary structure of pyridoxal phosphate binding site in the mitochondrial and extramitochondrial aspartate aminotransferases from pig heart muscle. Chymotryptic peptides.
Biochemistry8(8)3412-7(1969 Aug)
Morino Y,Watanabe T