PRKAG1 cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Gene Symbol	PRKAG1
Entrez Gene ID	414426
Full Name	protein kinase AMP-activated non-catalytic subunit gamma 1
Gene Type	protein-coding
Organism	Sus scrofa(pig)
Genome	Chromosome: 5 Map Location: 5\|5

mRNA and Protein(s)

mRNA	Protein	Name
XM_005655543.3	XP_005655600.1	5'-AMP-activated protein kinase subunit gamma-1 isoform X1
XM_021091200.1	XP_020946859.1	5'-AMP-activated protein kinase subunit gamma-1 isoform X1
XM_005655543.2	XP_005655600.1	5'-AMP-activated protein kinase subunit gamma-1 isoform X1
XM_021091201.1	XP_020946860.1	5'-AMP-activated protein kinase subunit gamma-1 isoform X1
NM_001001642.2	NP_001001642.1	5'-AMP-activated protein kinase subunit gamma-1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The halothane gene, energy metabolism, adenosine monophosphate-activated protein kinase, and glycolysis in postmortem pig longissimus dorsi muscle.
Shen QW, Underwood KR, Means WJ, McCormick RJ, Du M
Journal of animal science85(4)1054-61(2007 Apr)

PEDE (Pig EST Data Explorer) has been expanded into Pig Expression Data Explorer, including 10 147 porcine full-length cDNA sequences.
Uenishi H, Eguchi-Ogawa T, Shinkai H, Okumura N, Suzuki K, Toki D, Hamasima N, Awata T
Nucleic acids research35(Database issue)D650-3(2007 Jan)

Determination of PRKAG1 coding sequence and mapping of PRKAG1 and PRKAG2 relatively to porcine back fat thickness QTL.
Demeure O, Liaubet L, Riquet J, Milan D
Animal genetics35(2)123-5(2004 Apr)

Assignment of two isoforms of the AMP-activated protein kinase gamma subunits, PRKAG1 and PRKAG2 to porcine chromosomes 5 and 18, respectively by radiation hybrid panel mapping.
Haberkern G, Regenhard P, Ottzen-Schirakow G, Kalm E, Looft C
Cytogenetic and genome research106(1)142(2004)

PEDE (Pig EST Data Explorer): construction of a database for ESTs derived from porcine full-length cDNA libraries.
Uenishi H, Eguchi T, Suzuki K, Sawazaki T, Toki D, Shinkai H, Okumura N, Hamasima N, Awata T
Nucleic acids research32(Database issue)D484-8(2004 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

mapped on porcine chromosome 5
Title: Assignment of two isoforms of the AMP-activated protein kinase gamma subunits, PRKAG1 and PRKAG2 to porcine chromosomes 5 and 18, respectively by radiation hybrid panel mapping.

The following PRKAG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRKAG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSe09585	XM_005655543.3 Latest version!	Sus scrofa protein kinase AMP-activated non-catalytic subunit gamma 1 (PRKAG1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OSe09585	XM_021091201.1 Latest version!	Sus scrofa protein kinase AMP-activated non-catalytic subunit gamma 1 (PRKAG1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OSe09585	XM_021091200.1 Latest version!	Sus scrofa protein kinase AMP-activated non-catalytic subunit gamma 1 (PRKAG1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OSe00637	NM_001001642.2 Latest version!	Sus scrofa protein kinase AMP-activated non-catalytic subunit gamma 1 (PRKAG1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
View Old Accession Versions >>
OSe09585	XM_005655543.2	Sus scrofa protein kinase, AMP-activated, gamma 1 non-catalytic subunit (PRKAG1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRKAG1 ( XM_005655543.3 ) cDNA ORF clone, Sus scrofa(pig) -> XP_005655600.1 Sus scrofa protein kinase AMP-activated non-catalytic subunit gamma 1 (PRKAG1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OSe09585
Clone ID Related Accession (Same CDS sequence)	XM_005655543.3 , XM_021091200.1 , XM_005655543.2 , XM_021091201.1
Accession Version	XM_005655543.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	5'-AMP-activated protein kinase subunit gamma-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010447.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_005655543.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTCTC ATCGCTGCTA TGACCTGATT CCCACAAGCT CAAAATTGGT TGTATTTGAT 
ACCTCCCTGC AGGTGAAGAA AGCTTTCTTT GCTTTGGTGA CTAATGGTGT CCGAGCTGCC 
CCTTTATGGG ATAGTAAGAA GCAAAGTTTC GTGGGCATGC TGACCATCAC TGATTTCATC 
AATATCCTGC ACCGCTACTA TAAATCAGCC TTGGTACAGA TCTATGAGCT GGAAGAACAC 
AAGATAGAAA CTTGGAGAGA GGTGTACCTA CAGGACTCCT TTAAACCGCT TGTCTGCATT 
TCTCCTAATG CCAGCTTGTT TGATGCTGTC TCTTCATTAA TTCGAAACAA GATCCACAGG 
CTGCCAGTTA TTGACCCAGA GTCCGGGAAC ACCTTGTACA TCCTCACCCA CAAGCGCATC 
CTCAAGTTCC TCAAGCTGTT TATCACTGAG TTCCCCAAGC CCGAGTTCAT GTCTAAGTCT 
CTGGAAGAGC TACAGATTGG CACCTATGCC AACATTGCTA TGGTCCGCAC TACCACCCCT 
GTCTATGTGG CTCTGGGCAT CTTTGTACAG CACCGAGTCT CAGCCCTGCC AGTGGTGGAT 
GAAAAAGGGC GTGTGGTGGA CATCTACTCC AAGTTTGATG TTATCAACCT GGCAGCAGAA 
AAGACCTACA ACAACCTAGA TGTATCTGTG ACCAAAGCCC TGCAACATCG ATCACATTAC 
TTTGAAGGTG TCCTCAAGTG CTACCTGCAT GAGACCCTGG AAACCATCAT CAACAGGCTG 
GTAGAAGCAG AGGTTCACCG ACTTGTAGTG GTGGATGAGA ATGACGTGGT CAAGGGAATT 
GTATCGCTGT CTGACATCCT GCAGGCCCTG GTGCTCACAG GAGGAGAGAA GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005655600.1
CDS	177..1073
Translation	MKSHRCYDLIPTSSKLVVFDTSLQVKKAFFALVTNGVRAAPLWDSKKQSFVGMLTITDFINILHRYYKSALVQIYELEEHKIETWREVYLQDSFKPLVCISPNASLFDAVSSLIRNKIHRLPVIDPESGNTLYILTHKRILKFLKLFITEFPKPEFMSKSLEELQIGTYANIAMVRTTTPVYVALGIFVQHRVSALPVVDEKGRVVDIYSKFDVINLAAEKTYNNLDVSVTKALQHRSHYFEGVLKCYLHETLETIINRLVEAEVHRLVVVDENDVVKGIVSLSDILQALVLTGGEKP

Target ORF information:

RefSeq Version	XM_005655543.3
Organism	Sus scrofa(pig)
Definition	Sus scrofa protein kinase AMP-activated non-catalytic subunit gamma 1 (PRKAG1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005655543.3

ORF Insert Sequence:

ATGAAGTCTC ATCGCTGCTA TGACCTGATT CCCACAAGCT CAAAATTGGT TGTATTTGAT 
ACCTCCCTGC AGGTGAAGAA AGCTTTCTTT GCTTTGGTGA CTAATGGTGT CCGAGCTGCC 
CCTTTATGGG ATAGTAAGAA GCAAAGTTTC GTGGGCATGC TGACCATCAC TGATTTCATC 
AATATCCTGC ACCGCTACTA TAAATCAGCC TTGGTACAGA TCTATGAGCT GGAAGAACAC 
AAGATAGAAA CTTGGAGAGA GGTGTACCTA CAGGACTCCT TTAAACCGCT TGTCTGCATT 
TCTCCTAATG CCAGCTTGTT TGATGCTGTC TCTTCATTAA TTCGAAACAA GATCCACAGG 
CTGCCAGTTA TTGACCCAGA GTCCGGGAAC ACCTTGTACA TCCTCACCCA CAAGCGCATC 
CTCAAGTTCC TCAAGCTGTT TATCACTGAG TTCCCCAAGC CCGAGTTCAT GTCTAAGTCT 
CTGGAAGAGC TACAGATTGG CACCTATGCC AACATTGCTA TGGTCCGCAC TACCACCCCT 
GTCTATGTGG CTCTGGGCAT CTTTGTACAG CACCGAGTCT CAGCCCTGCC AGTGGTGGAT 
GAAAAAGGGC GTGTGGTGGA CATCTACTCC AAGTTTGATG TTATCAACCT GGCAGCAGAA 
AAGACCTACA ACAACCTAGA TGTATCTGTG ACCAAAGCCC TGCAACATCG ATCACATTAC 
TTTGAAGGTG TCCTCAAGTG CTACCTGCAT GAGACCCTGG AAACCATCAT CAACAGGCTG 
GTAGAAGCAG AGGTTCACCG ACTTGTAGTG GTGGATGAGA ATGACGTGGT CAAGGGAATT 
GTATCGCTGT CTGACATCCT GCAGGCCCTG GTGCTCACAG GAGGAGAGAA GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKAG1 ( XM_021091200.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020946859.1 Sus scrofa protein kinase AMP-activated non-catalytic subunit gamma 1 (PRKAG1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSe09585
Clone ID Related Accession (Same CDS sequence)	XM_005655543.3 , XM_021091200.1 , XM_005655543.2 , XM_021091201.1
Accession Version	XM_021091200.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	5'-AMP-activated protein kinase subunit gamma-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010447.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTCTC ATCGCTGCTA TGACCTGATT CCCACAAGCT CAAAATTGGT TGTATTTGAT 
ACCTCCCTGC AGGTGAAGAA AGCTTTCTTT GCTTTGGTGA CTAATGGTGT CCGAGCTGCC 
CCTTTATGGG ATAGTAAGAA GCAAAGTTTC GTGGGCATGC TGACCATCAC TGATTTCATC 
AATATCCTGC ACCGCTACTA TAAATCAGCC TTGGTACAGA TCTATGAGCT GGAAGAACAC 
AAGATAGAAA CTTGGAGAGA GGTGTACCTA CAGGACTCCT TTAAACCGCT TGTCTGCATT 
TCTCCTAATG CCAGCTTGTT TGATGCTGTC TCTTCATTAA TTCGAAACAA GATCCACAGG 
CTGCCAGTTA TTGACCCAGA GTCCGGGAAC ACCTTGTACA TCCTCACCCA CAAGCGCATC 
CTCAAGTTCC TCAAGCTGTT TATCACTGAG TTCCCCAAGC CCGAGTTCAT GTCTAAGTCT 
CTGGAAGAGC TACAGATTGG CACCTATGCC AACATTGCTA TGGTCCGCAC TACCACCCCT 
GTCTATGTGG CTCTGGGCAT CTTTGTACAG CACCGAGTCT CAGCCCTGCC AGTGGTGGAT 
GAAAAAGGGC GTGTGGTGGA CATCTACTCC AAGTTTGATG TTATCAACCT GGCAGCAGAA 
AAGACCTACA ACAACCTAGA TGTATCTGTG ACCAAAGCCC TGCAACATCG ATCACATTAC 
TTTGAAGGTG TCCTCAAGTG CTACCTGCAT GAGACCCTGG AAACCATCAT CAACAGGCTG 
GTAGAAGCAG AGGTTCACCG ACTTGTAGTG GTGGATGAGA ATGACGTGGT CAAGGGAATT 
GTATCGCTGT CTGACATCCT GCAGGCCCTG GTGCTCACAG GAGGAGAGAA GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020946859.1
CDS	275..1171
Translation	MKSHRCYDLIPTSSKLVVFDTSLQVKKAFFALVTNGVRAAPLWDSKKQSFVGMLTITDFINILHRYYKSALVQIYELEEHKIETWREVYLQDSFKPLVCISPNASLFDAVSSLIRNKIHRLPVIDPESGNTLYILTHKRILKFLKLFITEFPKPEFMSKSLEELQIGTYANIAMVRTTTPVYVALGIFVQHRVSALPVVDEKGRVVDIYSKFDVINLAAEKTYNNLDVSVTKALQHRSHYFEGVLKCYLHETLETIINRLVEAEVHRLVVVDENDVVKGIVSLSDILQALVLTGGEKP

Target ORF information:

RefSeq Version	XM_021091200.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa protein kinase AMP-activated non-catalytic subunit gamma 1 (PRKAG1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021091200.1

ORF Insert Sequence:

ATGAAGTCTC ATCGCTGCTA TGACCTGATT CCCACAAGCT CAAAATTGGT TGTATTTGAT 
ACCTCCCTGC AGGTGAAGAA AGCTTTCTTT GCTTTGGTGA CTAATGGTGT CCGAGCTGCC 
CCTTTATGGG ATAGTAAGAA GCAAAGTTTC GTGGGCATGC TGACCATCAC TGATTTCATC 
AATATCCTGC ACCGCTACTA TAAATCAGCC TTGGTACAGA TCTATGAGCT GGAAGAACAC 
AAGATAGAAA CTTGGAGAGA GGTGTACCTA CAGGACTCCT TTAAACCGCT TGTCTGCATT 
TCTCCTAATG CCAGCTTGTT TGATGCTGTC TCTTCATTAA TTCGAAACAA GATCCACAGG 
CTGCCAGTTA TTGACCCAGA GTCCGGGAAC ACCTTGTACA TCCTCACCCA CAAGCGCATC 
CTCAAGTTCC TCAAGCTGTT TATCACTGAG TTCCCCAAGC CCGAGTTCAT GTCTAAGTCT 
CTGGAAGAGC TACAGATTGG CACCTATGCC AACATTGCTA TGGTCCGCAC TACCACCCCT 
GTCTATGTGG CTCTGGGCAT CTTTGTACAG CACCGAGTCT CAGCCCTGCC AGTGGTGGAT 
GAAAAAGGGC GTGTGGTGGA CATCTACTCC AAGTTTGATG TTATCAACCT GGCAGCAGAA 
AAGACCTACA ACAACCTAGA TGTATCTGTG ACCAAAGCCC TGCAACATCG ATCACATTAC 
TTTGAAGGTG TCCTCAAGTG CTACCTGCAT GAGACCCTGG AAACCATCAT CAACAGGCTG 
GTAGAAGCAG AGGTTCACCG ACTTGTAGTG GTGGATGAGA ATGACGTGGT CAAGGGAATT 
GTATCGCTGT CTGACATCCT GCAGGCCCTG GTGCTCACAG GAGGAGAGAA GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKAG1 ( XM_005655543.2 ) cDNA ORF clone, Sus scrofa(pig) -> XP_005655600.1 Sus scrofa protein kinase, AMP-activated, gamma 1 non-catalytic subunit (PRKAG1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSe09585
Clone ID Related Accession (Same CDS sequence)	XM_005655543.3 , XM_021091200.1 , XM_005655543.2 , XM_021091201.1
Accession Version	XM_005655543.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	5'-AMP-activated protein kinase subunit gamma-1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003534741.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 11, 2015 this sequence version replaced XM_005655543.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTCTC ATCGCTGCTA TGACCTGATT CCCACAAGCT CAAAATTGGT TGTATTTGAT 
ACCTCCCTGC AGGTGAAGAA AGCTTTCTTT GCTTTGGTGA CTAATGGTGT CCGAGCTGCC 
CCTTTATGGG ATAGTAAGAA GCAAAGTTTC GTGGGCATGC TGACCATCAC TGATTTCATC 
AATATCCTGC ACCGCTACTA TAAATCAGCC TTGGTACAGA TCTATGAGCT GGAAGAACAC 
AAGATAGAAA CTTGGAGAGA GGTGTACCTA CAGGACTCCT TTAAACCGCT TGTCTGCATT 
TCTCCTAATG CCAGCTTGTT TGATGCTGTC TCTTCATTAA TTCGAAACAA GATCCACAGG 
CTGCCAGTTA TTGACCCAGA GTCCGGGAAC ACCTTGTACA TCCTCACCCA CAAGCGCATC 
CTCAAGTTCC TCAAGCTGTT TATCACTGAG TTCCCCAAGC CCGAGTTCAT GTCTAAGTCT 
CTGGAAGAGC TACAGATTGG CACCTATGCC AACATTGCTA TGGTCCGCAC TACCACCCCT 
GTCTATGTGG CTCTGGGCAT CTTTGTACAG CACCGAGTCT CAGCCCTGCC AGTGGTGGAT 
GAAAAAGGGC GTGTGGTGGA CATCTACTCC AAGTTTGATG TTATCAACCT GGCAGCAGAA 
AAGACCTACA ACAACCTAGA TGTATCTGTG ACCAAAGCCC TGCAACATCG ATCACATTAC 
TTTGAAGGTG TCCTCAAGTG CTACCTGCAT GAGACCCTGG AAACCATCAT CAACAGGCTG 
GTAGAAGCAG AGGTTCACCG ACTTGTAGTG GTGGATGAGA ATGACGTGGT CAAGGGAATT 
GTATCGCTGT CTGACATCCT GCAGGCCCTG GTGCTCACAG GAGGAGAGAA GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005655600.1
CDS	163..1059
Translation	MKSHRCYDLIPTSSKLVVFDTSLQVKKAFFALVTNGVRAAPLWDSKKQSFVGMLTITDFINILHRYYKSALVQIYELEEHKIETWREVYLQDSFKPLVCISPNASLFDAVSSLIRNKIHRLPVIDPESGNTLYILTHKRILKFLKLFITEFPKPEFMSKSLEELQIGTYANIAMVRTTTPVYVALGIFVQHRVSALPVVDEKGRVVDIYSKFDVINLAAEKTYNNLDVSVTKALQHRSHYFEGVLKCYLHETLETIINRLVEAEVHRLVVVDENDVVKGIVSLSDILQALVLTGGEKP

Target ORF information:

RefSeq Version	XM_005655543.2
Organism	Sus scrofa(Pig)
Definition	Sus scrofa protein kinase, AMP-activated, gamma 1 non-catalytic subunit (PRKAG1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005655543.2

ORF Insert Sequence:

ATGAAGTCTC ATCGCTGCTA TGACCTGATT CCCACAAGCT CAAAATTGGT TGTATTTGAT 
ACCTCCCTGC AGGTGAAGAA AGCTTTCTTT GCTTTGGTGA CTAATGGTGT CCGAGCTGCC 
CCTTTATGGG ATAGTAAGAA GCAAAGTTTC GTGGGCATGC TGACCATCAC TGATTTCATC 
AATATCCTGC ACCGCTACTA TAAATCAGCC TTGGTACAGA TCTATGAGCT GGAAGAACAC 
AAGATAGAAA CTTGGAGAGA GGTGTACCTA CAGGACTCCT TTAAACCGCT TGTCTGCATT 
TCTCCTAATG CCAGCTTGTT TGATGCTGTC TCTTCATTAA TTCGAAACAA GATCCACAGG 
CTGCCAGTTA TTGACCCAGA GTCCGGGAAC ACCTTGTACA TCCTCACCCA CAAGCGCATC 
CTCAAGTTCC TCAAGCTGTT TATCACTGAG TTCCCCAAGC CCGAGTTCAT GTCTAAGTCT 
CTGGAAGAGC TACAGATTGG CACCTATGCC AACATTGCTA TGGTCCGCAC TACCACCCCT 
GTCTATGTGG CTCTGGGCAT CTTTGTACAG CACCGAGTCT CAGCCCTGCC AGTGGTGGAT 
GAAAAAGGGC GTGTGGTGGA CATCTACTCC AAGTTTGATG TTATCAACCT GGCAGCAGAA 
AAGACCTACA ACAACCTAGA TGTATCTGTG ACCAAAGCCC TGCAACATCG ATCACATTAC 
TTTGAAGGTG TCCTCAAGTG CTACCTGCAT GAGACCCTGG AAACCATCAT CAACAGGCTG 
GTAGAAGCAG AGGTTCACCG ACTTGTAGTG GTGGATGAGA ATGACGTGGT CAAGGGAATT 
GTATCGCTGT CTGACATCCT GCAGGCCCTG GTGCTCACAG GAGGAGAGAA GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKAG1 ( XM_021091201.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020946860.1 Sus scrofa protein kinase AMP-activated non-catalytic subunit gamma 1 (PRKAG1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSe09585
Clone ID Related Accession (Same CDS sequence)	XM_005655543.3 , XM_021091200.1 , XM_005655543.2 , XM_021091201.1
Accession Version	XM_021091201.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	5'-AMP-activated protein kinase subunit gamma-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010447.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTCTC ATCGCTGCTA TGACCTGATT CCCACAAGCT CAAAATTGGT TGTATTTGAT 
ACCTCCCTGC AGGTGAAGAA AGCTTTCTTT GCTTTGGTGA CTAATGGTGT CCGAGCTGCC 
CCTTTATGGG ATAGTAAGAA GCAAAGTTTC GTGGGCATGC TGACCATCAC TGATTTCATC 
AATATCCTGC ACCGCTACTA TAAATCAGCC TTGGTACAGA TCTATGAGCT GGAAGAACAC 
AAGATAGAAA CTTGGAGAGA GGTGTACCTA CAGGACTCCT TTAAACCGCT TGTCTGCATT 
TCTCCTAATG CCAGCTTGTT TGATGCTGTC TCTTCATTAA TTCGAAACAA GATCCACAGG 
CTGCCAGTTA TTGACCCAGA GTCCGGGAAC ACCTTGTACA TCCTCACCCA CAAGCGCATC 
CTCAAGTTCC TCAAGCTGTT TATCACTGAG TTCCCCAAGC CCGAGTTCAT GTCTAAGTCT 
CTGGAAGAGC TACAGATTGG CACCTATGCC AACATTGCTA TGGTCCGCAC TACCACCCCT 
GTCTATGTGG CTCTGGGCAT CTTTGTACAG CACCGAGTCT CAGCCCTGCC AGTGGTGGAT 
GAAAAAGGGC GTGTGGTGGA CATCTACTCC AAGTTTGATG TTATCAACCT GGCAGCAGAA 
AAGACCTACA ACAACCTAGA TGTATCTGTG ACCAAAGCCC TGCAACATCG ATCACATTAC 
TTTGAAGGTG TCCTCAAGTG CTACCTGCAT GAGACCCTGG AAACCATCAT CAACAGGCTG 
GTAGAAGCAG AGGTTCACCG ACTTGTAGTG GTGGATGAGA ATGACGTGGT CAAGGGAATT 
GTATCGCTGT CTGACATCCT GCAGGCCCTG GTGCTCACAG GAGGAGAGAA GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020946860.1
CDS	376..1272
Translation	MKSHRCYDLIPTSSKLVVFDTSLQVKKAFFALVTNGVRAAPLWDSKKQSFVGMLTITDFINILHRYYKSALVQIYELEEHKIETWREVYLQDSFKPLVCISPNASLFDAVSSLIRNKIHRLPVIDPESGNTLYILTHKRILKFLKLFITEFPKPEFMSKSLEELQIGTYANIAMVRTTTPVYVALGIFVQHRVSALPVVDEKGRVVDIYSKFDVINLAAEKTYNNLDVSVTKALQHRSHYFEGVLKCYLHETLETIINRLVEAEVHRLVVVDENDVVKGIVSLSDILQALVLTGGEKP

Target ORF information:

RefSeq Version	XM_021091201.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa protein kinase AMP-activated non-catalytic subunit gamma 1 (PRKAG1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021091201.1

ORF Insert Sequence:

ATGAAGTCTC ATCGCTGCTA TGACCTGATT CCCACAAGCT CAAAATTGGT TGTATTTGAT 
ACCTCCCTGC AGGTGAAGAA AGCTTTCTTT GCTTTGGTGA CTAATGGTGT CCGAGCTGCC 
CCTTTATGGG ATAGTAAGAA GCAAAGTTTC GTGGGCATGC TGACCATCAC TGATTTCATC 
AATATCCTGC ACCGCTACTA TAAATCAGCC TTGGTACAGA TCTATGAGCT GGAAGAACAC 
AAGATAGAAA CTTGGAGAGA GGTGTACCTA CAGGACTCCT TTAAACCGCT TGTCTGCATT 
TCTCCTAATG CCAGCTTGTT TGATGCTGTC TCTTCATTAA TTCGAAACAA GATCCACAGG 
CTGCCAGTTA TTGACCCAGA GTCCGGGAAC ACCTTGTACA TCCTCACCCA CAAGCGCATC 
CTCAAGTTCC TCAAGCTGTT TATCACTGAG TTCCCCAAGC CCGAGTTCAT GTCTAAGTCT 
CTGGAAGAGC TACAGATTGG CACCTATGCC AACATTGCTA TGGTCCGCAC TACCACCCCT 
GTCTATGTGG CTCTGGGCAT CTTTGTACAG CACCGAGTCT CAGCCCTGCC AGTGGTGGAT 
GAAAAAGGGC GTGTGGTGGA CATCTACTCC AAGTTTGATG TTATCAACCT GGCAGCAGAA 
AAGACCTACA ACAACCTAGA TGTATCTGTG ACCAAAGCCC TGCAACATCG ATCACATTAC 
TTTGAAGGTG TCCTCAAGTG CTACCTGCAT GAGACCCTGG AAACCATCAT CAACAGGCTG 
GTAGAAGCAG AGGTTCACCG ACTTGTAGTG GTGGATGAGA ATGACGTGGT CAAGGGAATT 
GTATCGCTGT CTGACATCCT GCAGGCCCTG GTGCTCACAG GAGGAGAGAA GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKAG1 ( NM_001001642.2 ) cDNA ORF clone, Sus scrofa(pig) -> NP_001001642.1 Sus scrofa protein kinase AMP-activated non-catalytic subunit gamma 1 (PRKAG1), mRNA.

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CloneID	OSe00637
Clone ID Related Accession (Same CDS sequence)	NM_001001642.2
Accession Version	NM_001001642.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Sus scrofa(pig)
Product	5'-AMP-activated protein kinase subunit gamma-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY260940.1. On Aug 31, 2012 this sequence version replaced NM_001001642.1. ##Evidence-Data-START## Transcript exon combination :: AY260940.1, AK233656.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02584789 [ECO:0000348] ##Evidence-Data-END##

ATGGAGACGG TCACATCTTC AGATAGCTCC TCAGCTGTGG AAAATGAGCA TCCTCAAGAC 
ACCCCAGAAT CCAACAATAG CGTGTACACT TCCTTCATGA AGTCTCATCG CTGCTATGAC 
CTGATTCCCA CAAGCTCAAA ATTGGTTGTA TTTGATACCT CCCTGCAGGT GAAGAAAGCT 
TTCTTTGCTT TGGTGACTAA TGGTGTCCGA GCTGCCCCTT TATGGGATAG TAAGAAGCAA 
AGTTTCGTGG GCATGCTGAC CATCACTGAT TTCATCAATA TCCTGCACCG CTACTATAAA 
TCAGCCTTGG TACAGATCTA TGAGCTGGAA GAACACAAGA TAGAAACTTG GAGAGAGGTG 
TACCTACAGG ACTCCTTTAA ACCGCTTGTC TGCATTTCTC CTAATGCCAG CTTGTTTGAT 
GCTGTCTCTT CATTAATTCG AAACAAGATC CACAGGCTGC CAGTTATTGA CCCAGAGTCC 
GGGAACACCT TGTACATCCT CACCCACAAG CGCATCCTCA AGTTCCTCAA GCTGTTTATC 
ACTGAGTTCC CCAAGCCCGA GTTCATGTCT AAGTCTCTGG AAGAGCTACA GATTGGCACC 
TATGCCAACA TTGCTATGGT CCGCACTACC ACCCCTGTCT ATGTGGCTCT GGGCATCTTT 
GTACAGCACC GAGTCTCAGC CCTGCCAGTG GTGGATGAAA AAGGGCGTGT GGTGGACATC 
TACTCCAAGT TTGATGTTAT CAACCTGGCA GCAGAAAAGA CCTACAACAA CCTAGATGTA 
TCTGTGACCA AAGCCCTGCA GCATCGATCA CATTACTTTG AAGGTGTCCT CAAGTGCTAC 
CTGCATGAGA CCCTGGAAAC CATCATCAAC AGGCTGGTAG AAGCAGAGGT TCACCGACTT 
GTAGTGGTGG ATGAGAATGA CGTGGTCAAG GGAATTGTAT CGCTGTCTGA CATCCTGCAG 
GCCCTGGTGC TCACAGGAGG AGAGAAGCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001001642.1
CDS	41..1033
Translation	METVTSSDSSSAVENEHPQDTPESNNSVYTSFMKSHRCYDLIPTSSKLVVFDTSLQVKKAFFALVTNGVRAAPLWDSKKQSFVGMLTITDFINILHRYYKSALVQIYELEEHKIETWREVYLQDSFKPLVCISPNASLFDAVSSLIRNKIHRLPVIDPESGNTLYILTHKRILKFLKLFITEFPKPEFMSKSLEELQIGTYANIAMVRTTTPVYVALGIFVQHRVSALPVVDEKGRVVDIYSKFDVINLAAEKTYNNLDVSVTKALQHRSHYFEGVLKCYLHETLETIINRLVEAEVHRLVVVDENDVVKGIVSLSDILQALVLTGGEKP

Target ORF information:

RefSeq Version	NM_001001642.2
Organism	Sus scrofa(pig)
Definition	Sus scrofa protein kinase AMP-activated non-catalytic subunit gamma 1 (PRKAG1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001001642.2

ORF Insert Sequence:

ATGGAGACGG TCACATCTTC AGATAGCTCC TCAGCTGTGG AAAATGAGCA TCCTCAAGAC 
ACCCCAGAAT CCAACAATAG CGTGTACACT TCCTTCATGA AGTCTCATCG CTGCTATGAC 
CTGATTCCCA CAAGCTCAAA ATTGGTTGTA TTTGATACCT CCCTGCAGGT GAAGAAAGCT 
TTCTTTGCTT TGGTGACTAA TGGTGTCCGA GCTGCCCCTT TATGGGATAG TAAGAAGCAA 
AGTTTCGTGG GCATGCTGAC CATCACTGAT TTCATCAATA TCCTGCACCG CTACTATAAA 
TCAGCCTTGG TACAGATCTA TGAGCTGGAA GAACACAAGA TAGAAACTTG GAGAGAGGTG 
TACCTACAGG ACTCCTTTAA ACCGCTTGTC TGCATTTCTC CTAATGCCAG CTTGTTTGAT 
GCTGTCTCTT CATTAATTCG AAACAAGATC CACAGGCTGC CAGTTATTGA CCCAGAGTCC 
GGGAACACCT TGTACATCCT CACCCACAAG CGCATCCTCA AGTTCCTCAA GCTGTTTATC 
ACTGAGTTCC CCAAGCCCGA GTTCATGTCT AAGTCTCTGG AAGAGCTACA GATTGGCACC 
TATGCCAACA TTGCTATGGT CCGCACTACC ACCCCTGTCT ATGTGGCTCT GGGCATCTTT 
GTACAGCACC GAGTCTCAGC CCTGCCAGTG GTGGATGAAA AAGGGCGTGT GGTGGACATC 
TACTCCAAGT TTGATGTTAT CAACCTGGCA GCAGAAAAGA CCTACAACAA CCTAGATGTA 
TCTGTGACCA AAGCCCTGCA GCATCGATCA CATTACTTTG AAGGTGTCCT CAAGTGCTAC 
CTGCATGAGA CCCTGGAAAC CATCATCAAC AGGCTGGTAG AAGCAGAGGT TCACCGACTT 
GTAGTGGTGG ATGAGAATGA CGTGGTCAAG GGAATTGTAT CGCTGTCTGA CATCCTGCAG 
GCCCTGGTGC TCACAGGAGG AGAGAAGCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The halothane gene, energy metabolism, adenosine monophosphate-activated protein kinase, and glycolysis in postmortem pig longissimus dorsi muscle.
Journal of animal science85(4)1054-61(2007 Apr)
Shen QW,Underwood KR,Means WJ,McCormick RJ,Du M

PEDE (Pig EST Data Explorer) has been expanded into Pig Expression Data Explorer, including 10 147 porcine full-length cDNA sequences.
Nucleic acids research35(Database issue)D650-3(2007 Jan)
Uenishi H,Eguchi-Ogawa T,Shinkai H,Okumura N,Suzuki K,Toki D,Hamasima N,Awata T

Determination of PRKAG1 coding sequence and mapping of PRKAG1 and PRKAG2 relatively to porcine back fat thickness QTL.
Animal genetics35(2)123-5(2004 Apr)
Demeure O,Liaubet L,Riquet J,Milan D

Assignment of two isoforms of the AMP-activated protein kinase gamma subunits, PRKAG1 and PRKAG2 to porcine chromosomes 5 and 18, respectively by radiation hybrid panel mapping.
Cytogenetic and genome research106(1)142(2004)
Haberkern G,Regenhard P,Ottzen-Schirakow G,Kalm E,Looft C

PEDE (Pig EST Data Explorer): construction of a database for ESTs derived from porcine full-length cDNA libraries.
Nucleic acids research32(Database issue)D484-8(2004 Jan)
Uenishi H,Eguchi T,Suzuki K,Sawazaki T,Toki D,Shinkai H,Okumura N,Hamasima N,Awata T

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PRKAG1 cDNA ORF clone, Sus scrofa(pig)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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