tet3 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

  • Gene

  • Clones

  • gRNAs

The following tet3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tet3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa53115 XM_031897483.1
Latest version!
Xenopus tropicalis tet methylcytosine dioxygenase 3 (tet3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa53115 XM_031897482.1
Latest version!
Xenopus tropicalis tet methylcytosine dioxygenase 3 (tet3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa53115 XM_031897485.1
Latest version!
Xenopus tropicalis tet methylcytosine dioxygenase 3 (tet3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa53116 XM_031897486.1
Latest version!
Xenopus tropicalis tet methylcytosine dioxygenase 3 (tet3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa53117 XM_031897489.1
Latest version!
Xenopus tropicalis tet methylcytosine dioxygenase 3 (tet3), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa53117 XM_031897487.1
Latest version!
Xenopus tropicalis tet methylcytosine dioxygenase 3 (tet3), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa53117 XM_031897488.1
Latest version!
Xenopus tropicalis tet methylcytosine dioxygenase 3 (tet3), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa53117 XM_031897490.1
Latest version!
Xenopus tropicalis tet methylcytosine dioxygenase 3 (tet3), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa22183 NM_001097187.1
Latest version!
Xenopus tropicalis tet methylcytosine dioxygenase 3 (tet3), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa53115
    Clone ID Related Accession (Same CDS sequence) XM_031897483.1 , XM_031897482.1 , XM_031897485.1
    Accession Version XM_031897483.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5799bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product methylcytosine dioxygenase tet3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGGGGATC TCCCGTACGT TGGCTGTACC ATGATGGATA CTCAGCCCGC GCCAGTTCCA 
    CATGTGCTGC CCCAAGATGT ATATGAATTT CCTGATGACC AAGAGTCCCT TGGGAGATTA
    AGAGTCTCAG AGATGCCAGC GGAGCTGAAT GGTGGTGGTG GTGGTGGCAG TGCAGCTGCC
    TTTGCCATGG AACTTCCAGA GCAATCTAAT AAAAAAAGGA AGCGTTGTGG GGTCTGTGTG
    CCATGTCTAA GGAAGGAACC ATGTGGAGCC TGTTACAACT GTGTGAACCG GAGTACCAGC
    CACCAGATAT GCAAGATGCG AAAATGCGAG CAGCTCAAAA AGAAAAGAGT GGTCCCCATG
    AAAGGAGTGG AGACTGTAAA TAACGACGGC TCCAAGAATC AGGCAAAAGA ACAAGTGCCC
    AGTGTGAAGA ACTGTTCTGA AAGCATTTTA GTTGATGGAC CCAAAACAGA CCAAATGGAA
    GCAGGGCCAG TTAACCATGT TCAAGAAGGA AGGTTAAAGC AGGAATGTGA CTCTACCCTC
    CCAAGTAAGG GATGTGAGGA TCTAGCAAAC CAATTATTAA TGGAAGCAAA TTCGTGGCTA
    AGCAATACAG CTGCTCCTCA GGATCCCTGC AACAAGCTCA ACTGGGATAA ACCCACCATT
    CCCAACCATG CTGCTAATAA CAATTCAAAC CTAGAAGATG CCAAAAATCT AGTTGCATTC
    TCTGCTGTGG CTGAGGCTAT GTCTACCTAT GGCATGCCTG CATCAGGGAC CCCCTCTTCG
    GTATCACTGC AGCTATATGA AAAGTTTAAT TATGAGACAA ACAGGGACAA CTCTGGGCAC
    TTGGAAGGCA ATGCTCCATC ATGTCCCGAA GACCTAAACA CTCTAAAAGC AGCCCTTGCA
    CTTGCAAAGC ATGGCGTGAA ACCACCTAAC TGTAACTGTG ACGGCCCTGA GTGCCCGGAC
    TATCTAGAAT GGCTGGAAAA TAAAATAAAA TCTACAGTTA AAGGTTCTCA GGAGAGCCCA
    TTCCCTAATC TGGGTCAGGT CAGTAAAGAG CTGGTGCAAA AACAATACCC TAAAGAACAG
    GTGCTAAATC TAGAAAATAA AAATTCAACA TGTCCTTCAG GTAACCTTCC CTTTTCTCAA
    AATGCACTGA GTCTTGCTAA AGAAAAAAAT ATAAGTCTGC AAACGGCCAT TGCCATTGAA
    GCTCTTACAC AACTATCATC TGCACTTCCA CAGACAAACA ATGAATGCCC CAATGCTCCT
    TCCCAACCTT TAATAAACCC TCATGATCAG TTAACTCATT TCCCCTCTGC TAAAGGCAAC
    CAGTTACCAA TGTTACCAGT GGCCCGCAAT GAACTGTTCC AGAATCAGCA GTCCCAATTA
    TACACTGGCA AAAATGCTCT TCCTGTGCCC CAATCTCCAA GGCAAACTTC TTGGGAGCAG
    AACAAGAAAT CTAGTTACCA AGAAGGACAA TACATTCCTG AAAATTTATC ACATAGTTCT
    TCTGTGTTGC CCAGTGATGC TTCTACCCCA CAAAAACCTG AATTCCTTCA GCAGTGGGTC
    CAAAATGCAG ATCTTTTGAA GTCTCCATCT GATCCTATGA CTGGGTTAAA GCAATTGCTT
    GGGAACACAG ATGAGTACAT CAAGTCTGTG TTTAAAGGGC CAGAGGCTTT GCCCAACAAA
    AAAAATGTGA AGCCCAAACA TACCATTAAA TCCATAAAAA AAGAATCCAC CGAGTTTCTC
    AAAATGTCAC CAGATCAACA GCTCTCTCAG CTTCTTCAAA CAAATGAATT TCATCGTAAT
    ACCCAGGCTG CACTTCAGCA GCATCTCCAC CATAAACGCA ATCTATTTGT GGACCCAAAT
    GCTATGGAAG CATGTACCCA AGAACAGCAA AACTGGTGGG TGCCGAGTTC TCAACAAGCT
    CCAGTTTCCA AGACAACAGA GAAACCTGTA AAAGAGAGGA AGAAAAGGCG ACAGTCTCCT
    TCACAAAAGC AAGTGGAACC AAAACCGAAA CCTCAACGTA AGCAAGTGCA AATAAAGAAA
    CCCAAGGTGA AGGAAGGAAG TGCAGTCTTC ATGCCTGTGA GCCAAATAAG CCTGGATACT
    TTTCGCAGAG TTGAAAAAGA AGAGAACCAG GGGAAAGAAA TGGATGCAGA GAACTCTCTT
    CCTAATAACG TACAAACAGA ACTACTTGAA TCCCAGTCTT TACAACTGAC TGGATCACAG
    GCAAATCCTG ATGACAGAAA GACTGTAAAC ACTCAGGAAA TGTGCAATGA AAATCAGTCT
    AACATTGGGA AGGCCAATAA CTTTGCACTT TGTGTCAATC GGGCTAATTC TTTTGTTGCA
    AAAGACCAAT GTCCAACACC TTCCACACAT GATACCAGCT CTAGCTCTGG ACAAGGTGAT
    TCTGCCAACC AGCACCACAC AAATGTGAGT GATGTTCCTG GGCAAAATGA CCTTAGCTGC
    CTTGATGACA AGTTGGAAGA TCTAATAAGG CAGTTTGAAG CTGAGTTTGG GGAAGATTTC
    TCTCTACCTG GTTCTGCGGT CCCCTCTCAG AATGGTGAAG GACCGCCAAA ACAAACACCA
    TCAGGAGATC CACAATTTAA ACTGCCCTTT CCTTCCCAGT TGTTACCACC TGAGAATTCC
    ACAAAGCCTG CCACCCATTC CAACCCTGCA CTTTCAAATA ATCCTGTATC ACGTGAAGTT
    TCTAATAACC TTGATAGTCT GTTCTCATCA AAGTCTCCCA AGCAGATAAA AATCGAATCT
    TCTGGTGCTA TAACAGTGGT GTCTACCACA TGCTTCTACT CAGAGGAAAA CCAACATCTT
    GATGGAACTC CTACAAAAAG TGACCTGCCT TTCAATCCTA CTCTTAGTGG GTTTTTGGAC
    TCGCCTCTCA AATATCTGAC ATCTCCAACC AAGAGCTTAA TAGATACACC AGCTAAAAAG
    GCTCAAGCAG AATTTCCAAC ATGCGACTGT GTTGAACAAA TTAATGAAAA AGATGAAGGC
    CCATATTATA CACATCTGGG TTCTGGACCA ACAGTGGCTT CTATCAGAGA GCTAATGGAA
    GAGCGGTTTG GACAAAAAGG GGATGCAATC CGCATTGAAA AGGTGATCTA CACAGGAAAA
    GAAGGAAAAA GCTCAAGGGG TTGTCCTATT GCAAAATGGG TCATTCGCCG TCAGAGCGAG
    GATGAGAAGT TGATGTGCCT TGTCCGGCAA CGGGCAGGCC ACCATTGTGA AAATGCAGTC
    ATCATTATCT TAATTATGGC CTGGGAAGGA ATTCCTCGCT CTCTAGGAGA TTCTCTCTAC
    AACGACATCA CTGAGACAAT CACCAAGTAT GGAAATCCAA CATCACGGAG ATGTGGACTG
    AATGATGATC GAACCTGTGC ATGCCAAGGC AAGGACCCAA ATACCTGTGG TGCTTCTTTC
    TCTTTTGGGT GCTCATGGAG CATGTACTTT AATGGTTGCA AGTATGCTCG TAGCAAGACC
    CCCCGGAAGT TCCGACTTAT AGGAGAAAAT CCCAAGGAGG AAGATGGCCT GAAGGATAAC
    TTTCAGAATC TAGCTACAAA GGTTGCTCCA GTGTACAAGA TGCTTGCTCC TCAGGCTTAT
    CAAAATCAGG TTAACAATGA GGATATTGCA ATAGATTGTC GGCTAGGTCT CAAGGAGGGG
    CGTCCTTTCT CTGGGGTGAC TGCATGCATG GATTTCTGTG CCCATGCCCA CAAAGACCAG
    CACAATCTAT ACAATGGATG CACTGTGGTC TGCACTCTAA CTAAGGAGGA TAACCGTATG
    ATTGGCAGGG TTGCTGAGGA TGAGCAGCTC CATGTACTTC CACTGTACAA GGTTTCCACA
    ACTGATGAAT TTGGGAGTGA GGAGGGTCAA CTTGAAAAGA TAAAGAAAGG AGGAATCCAC
    GTGTTGAGCT CTTTTCCCCG GGAGGTACGC AAGCTTTCAG AACCAGCAAA GTCTTGCAGG
    CAGAGACAAC TAGAAGCCAA GAAGGCCGCA GCCGAAAAGA AGAAACTTCA AAAGGAGAAA
    CTGGTCTCTC CGGATAAGAC CAAGCAGGAG CCATCAGACA AAAAGACGTG CCAGCAAAAC
    CCAGGTGTTC CTCAGCAACA GACAAAGCCT TGTATAAAAG TGGAGCCTTC CAATCACTAC
    AATAACTTTA AGTACAATGG AAATGGAGTT GTGGAGAGCT ACTCAGTGTT GGGCAGCTGC
    AGGCCGTCCG ACCCTTACAG CATGAACAGC GTTTACTCCT ATCATTCTTT CTATGCACAA
    CCTAACCTGC CTTCCGTGAA TGGCTTTCAT TCAAAATATG CTCTTCCTCC CTTTGGATTT
    TATGGCTTTC CTAACAATCC TGTGGTTCCC AACCAGTTCA TGAATTATGG TACAAGTGAT
    GCAAGGAACA GTGGATGGAT GAATAATTGT TTTGAGAAAA AGCCAGAGCT TCAGTCACTG
    GCAGATGGAA TGAATCAAAG TTACGGGAGT GAGTTATCAG AGCAGAGTTT TAGGAGATCA
    AGTGAGGTAC CACACCACTA CAGCCTCCAA AATCCCAGCA GTCAAAAATC AGTTAATGTT
    CCCCACCGAA CTACTCCTGC ACCAGTGGAG ACTACTCCAT ATTCTAATCT TCCCTGTTAT
    AACAAAGTCA TTAAAAAGGA GCCAGGTAGT GATCCACTTG TAGATTCTTT CCAGAGAGCA
    AATAGTGTCC ATTCACATAG CCCAGGAGTT AATCACTCAT TACAGGCCAG TGATCTGCCC
    ATCTCATACA AAGCTAATGG TGCTCTATCA TCATCAGGGA GGACTAATGC AGAAAGTCCG
    TGCAGTATGT TTATGCCCAA TGATAAAAAT GGTCTAGAAA AGAAAGATTA TTTTGGAGTC
    CATTCGAATG CCCCTGGTCT GAAGGACAAG CAGTGGCCTC CCTATGGAAC TGATGTTTCA
    GTGAGACAAC ATGATTCACT TGACTCGCAG TCTCCAGGAA AAGTGTGGAG CTCATGCAAG
    CTGAGTGACA GTTCCGCTGC CCTGCCCTCC TCTGCAAGTA CACAGGATAA GAACTGGAAT
    GGTAGACAGG TCAGTCTTAA TCAGGGGATG AAGGAGTCTG CGCTGTTCCA AGAAAAACTC
    TGGAATTCAG TTGCTGCCAG TGACAGGTGC AGTGCCACGC CCAGTGACAG AAGTAGCATC
    ACCCCCTGTT CTGAGCTTCA GGACAAGAAC TGGGGATCAT TTCCTAATCC AACAGTAAAC
    TCTTTGAAGA CTGACTCCAG CCAGAACCAC TGGGATCCCT ATAGCCTAGA TGATAATATG
    GATGATGGCC AATCAAAGAG TGTAAAAGAG GAGGATGATG AGGAGATCTG GTCTGATAGT
    GAGCACAACT TTCTGGATGA GAATATCGGA GGTGTGGCTG TAGCCCCAGG ACATGGCTCT
    ATCCTAATTG AATGTGCAAG GAGAGAGCTC CATGCTACCA CTCCACTGAA AAAGCCCAAC
    AGATGTCATC CAACTCGAAT CTCTCTTGTA TTCTACCAAC ACAAGAACTT AAACCAACCT
    AACCATGGAT TGGCACTTTG GGAGGCCAAA ATGAAGCAGT TGGCAGAAAG GGCACGTGCA
    AGGGAGGAAG AGGCTGCCAA GCTAGGTATC AAACAGGAAG TAAAAAGTTT GGGTAAAAAG
    AGGAAGTGGG GTGGTGCTGC CACTACAGAA ACCCCACCAG TTGAGAAGAA AGACTATACC
    CCTACCCGAC AGGCAGCTAC AATTTTAACA GATTCTGCTA CCACTTCATT CTCCTATGCC
    TATACCAAGG TTACGGGACC ATACAGTCGC TTTATATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753343.1
    CDS451..6249
    Translation

    Target ORF information:

    RefSeq Version XM_031897483.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis tet methylcytosine dioxygenase 3 (tet3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897483.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGGGGATC TCCCGTACGT TGGCTGTACC ATGATGGATA CTCAGCCCGC GCCAGTTCCA 
    CATGTGCTGC CCCAAGATGT ATATGAATTT CCTGATGACC AAGAGTCCCT TGGGAGATTA
    AGAGTCTCAG AGATGCCAGC GGAGCTGAAT GGTGGTGGTG GTGGTGGCAG TGCAGCTGCC
    TTTGCCATGG AACTTCCAGA GCAATCTAAT AAAAAAAGGA AGCGTTGTGG GGTCTGTGTG
    CCATGTCTAA GGAAGGAACC ATGTGGAGCC TGTTACAACT GTGTGAACCG GAGTACCAGC
    CACCAGATAT GCAAGATGCG AAAATGCGAG CAGCTCAAAA AGAAAAGAGT GGTCCCCATG
    AAAGGAGTGG AGACTGTAAA TAACGACGGC TCCAAGAATC AGGCAAAAGA ACAAGTGCCC
    AGTGTGAAGA ACTGTTCTGA AAGCATTTTA GTTGATGGAC CCAAAACAGA CCAAATGGAA
    GCAGGGCCAG TTAACCATGT TCAAGAAGGA AGGTTAAAGC AGGAATGTGA CTCTACCCTC
    CCAAGTAAGG GATGTGAGGA TCTAGCAAAC CAATTATTAA TGGAAGCAAA TTCGTGGCTA
    AGCAATACAG CTGCTCCTCA GGATCCCTGC AACAAGCTCA ACTGGGATAA ACCCACCATT
    CCCAACCATG CTGCTAATAA CAATTCAAAC CTAGAAGATG CCAAAAATCT AGTTGCATTC
    TCTGCTGTGG CTGAGGCTAT GTCTACCTAT GGCATGCCTG CATCAGGGAC CCCCTCTTCG
    GTATCACTGC AGCTATATGA AAAGTTTAAT TATGAGACAA ACAGGGACAA CTCTGGGCAC
    TTGGAAGGCA ATGCTCCATC ATGTCCCGAA GACCTAAACA CTCTAAAAGC AGCCCTTGCA
    CTTGCAAAGC ATGGCGTGAA ACCACCTAAC TGTAACTGTG ACGGCCCTGA GTGCCCGGAC
    TATCTAGAAT GGCTGGAAAA TAAAATAAAA TCTACAGTTA AAGGTTCTCA GGAGAGCCCA
    TTCCCTAATC TGGGTCAGGT CAGTAAAGAG CTGGTGCAAA AACAATACCC TAAAGAACAG
    GTGCTAAATC TAGAAAATAA AAATTCAACA TGTCCTTCAG GTAACCTTCC CTTTTCTCAA
    AATGCACTGA GTCTTGCTAA AGAAAAAAAT ATAAGTCTGC AAACGGCCAT TGCCATTGAA
    GCTCTTACAC AACTATCATC TGCACTTCCA CAGACAAACA ATGAATGCCC CAATGCTCCT
    TCCCAACCTT TAATAAACCC TCATGATCAG TTAACTCATT TCCCCTCTGC TAAAGGCAAC
    CAGTTACCAA TGTTACCAGT GGCCCGCAAT GAACTGTTCC AGAATCAGCA GTCCCAATTA
    TACACTGGCA AAAATGCTCT TCCTGTGCCC CAATCTCCAA GGCAAACTTC TTGGGAGCAG
    AACAAGAAAT CTAGTTACCA AGAAGGACAA TACATTCCTG AAAATTTATC ACATAGTTCT
    TCTGTGTTGC CCAGTGATGC TTCTACCCCA CAAAAACCTG AATTCCTTCA GCAGTGGGTC
    CAAAATGCAG ATCTTTTGAA GTCTCCATCT GATCCTATGA CTGGGTTAAA GCAATTGCTT
    GGGAACACAG ATGAGTACAT CAAGTCTGTG TTTAAAGGGC CAGAGGCTTT GCCCAACAAA
    AAAAATGTGA AGCCCAAACA TACCATTAAA TCCATAAAAA AAGAATCCAC CGAGTTTCTC
    AAAATGTCAC CAGATCAACA GCTCTCTCAG CTTCTTCAAA CAAATGAATT TCATCGTAAT
    ACCCAGGCTG CACTTCAGCA GCATCTCCAC CATAAACGCA ATCTATTTGT GGACCCAAAT
    GCTATGGAAG CATGTACCCA AGAACAGCAA AACTGGTGGG TGCCGAGTTC TCAACAAGCT
    CCAGTTTCCA AGACAACAGA GAAACCTGTA AAAGAGAGGA AGAAAAGGCG ACAGTCTCCT
    TCACAAAAGC AAGTGGAACC AAAACCGAAA CCTCAACGTA AGCAAGTGCA AATAAAGAAA
    CCCAAGGTGA AGGAAGGAAG TGCAGTCTTC ATGCCTGTGA GCCAAATAAG CCTGGATACT
    TTTCGCAGAG TTGAAAAAGA AGAGAACCAG GGGAAAGAAA TGGATGCAGA GAACTCTCTT
    CCTAATAACG TACAAACAGA ACTACTTGAA TCCCAGTCTT TACAACTGAC TGGATCACAG
    GCAAATCCTG ATGACAGAAA GACTGTAAAC ACTCAGGAAA TGTGCAATGA AAATCAGTCT
    AACATTGGGA AGGCCAATAA CTTTGCACTT TGTGTCAATC GGGCTAATTC TTTTGTTGCA
    AAAGACCAAT GTCCAACACC TTCCACACAT GATACCAGCT CTAGCTCTGG ACAAGGTGAT
    TCTGCCAACC AGCACCACAC AAATGTGAGT GATGTTCCTG GGCAAAATGA CCTTAGCTGC
    CTTGATGACA AGTTGGAAGA TCTAATAAGG CAGTTTGAAG CTGAGTTTGG GGAAGATTTC
    TCTCTACCTG GTTCTGCGGT CCCCTCTCAG AATGGTGAAG GACCGCCAAA ACAAACACCA
    TCAGGAGATC CACAATTTAA ACTGCCCTTT CCTTCCCAGT TGTTACCACC TGAGAATTCC
    ACAAAGCCTG CCACCCATTC CAACCCTGCA CTTTCAAATA ATCCTGTATC ACGTGAAGTT
    TCTAATAACC TTGATAGTCT GTTCTCATCA AAGTCTCCCA AGCAGATAAA AATCGAATCT
    TCTGGTGCTA TAACAGTGGT GTCTACCACA TGCTTCTACT CAGAGGAAAA CCAACATCTT
    GATGGAACTC CTACAAAAAG TGACCTGCCT TTCAATCCTA CTCTTAGTGG GTTTTTGGAC
    TCGCCTCTCA AATATCTGAC ATCTCCAACC AAGAGCTTAA TAGATACACC AGCTAAAAAG
    GCTCAAGCAG AATTTCCAAC ATGCGACTGT GTTGAACAAA TTAATGAAAA AGATGAAGGC
    CCATATTATA CACATCTGGG TTCTGGACCA ACAGTGGCTT CTATCAGAGA GCTAATGGAA
    GAGCGGTTTG GACAAAAAGG GGATGCAATC CGCATTGAAA AGGTGATCTA CACAGGAAAA
    GAAGGAAAAA GCTCAAGGGG TTGTCCTATT GCAAAATGGG TCATTCGCCG TCAGAGCGAG
    GATGAGAAGT TGATGTGCCT TGTCCGGCAA CGGGCAGGCC ACCATTGTGA AAATGCAGTC
    ATCATTATCT TAATTATGGC CTGGGAAGGA ATTCCTCGCT CTCTAGGAGA TTCTCTCTAC
    AACGACATCA CTGAGACAAT CACCAAGTAT GGAAATCCAA CATCACGGAG ATGTGGACTG
    AATGATGATC GAACCTGTGC ATGCCAAGGC AAGGACCCAA ATACCTGTGG TGCTTCTTTC
    TCTTTTGGGT GCTCATGGAG CATGTACTTT AATGGTTGCA AGTATGCTCG TAGCAAGACC
    CCCCGGAAGT TCCGACTTAT AGGAGAAAAT CCCAAGGAGG AAGATGGCCT GAAGGATAAC
    TTTCAGAATC TAGCTACAAA GGTTGCTCCA GTGTACAAGA TGCTTGCTCC TCAGGCTTAT
    CAAAATCAGG TTAACAATGA GGATATTGCA ATAGATTGTC GGCTAGGTCT CAAGGAGGGG
    CGTCCTTTCT CTGGGGTGAC TGCATGCATG GATTTCTGTG CCCATGCCCA CAAAGACCAG
    CACAATCTAT ACAATGGATG CACTGTGGTC TGCACTCTAA CTAAGGAGGA TAACCGTATG
    ATTGGCAGGG TTGCTGAGGA TGAGCAGCTC CATGTACTTC CACTGTACAA GGTTTCCACA
    ACTGATGAAT TTGGGAGTGA GGAGGGTCAA CTTGAAAAGA TAAAGAAAGG AGGAATCCAC
    GTGTTGAGCT CTTTTCCCCG GGAGGTACGC AAGCTTTCAG AACCAGCAAA GTCTTGCAGG
    CAGAGACAAC TAGAAGCCAA GAAGGCCGCA GCCGAAAAGA AGAAACTTCA AAAGGAGAAA
    CTGGTCTCTC CGGATAAGAC CAAGCAGGAG CCATCAGACA AAAAGACGTG CCAGCAAAAC
    CCAGGTGTTC CTCAGCAACA GACAAAGCCT TGTATAAAAG TGGAGCCTTC CAATCACTAC
    AATAACTTTA AGTACAATGG AAATGGAGTT GTGGAGAGCT ACTCAGTGTT GGGCAGCTGC
    AGGCCGTCCG ACCCTTACAG CATGAACAGC GTTTACTCCT ATCATTCTTT CTATGCACAA
    CCTAACCTGC CTTCCGTGAA TGGCTTTCAT TCAAAATATG CTCTTCCTCC CTTTGGATTT
    TATGGCTTTC CTAACAATCC TGTGGTTCCC AACCAGTTCA TGAATTATGG TACAAGTGAT
    GCAAGGAACA GTGGATGGAT GAATAATTGT TTTGAGAAAA AGCCAGAGCT TCAGTCACTG
    GCAGATGGAA TGAATCAAAG TTACGGGAGT GAGTTATCAG AGCAGAGTTT TAGGAGATCA
    AGTGAGGTAC CACACCACTA CAGCCTCCAA AATCCCAGCA GTCAAAAATC AGTTAATGTT
    CCCCACCGAA CTACTCCTGC ACCAGTGGAG ACTACTCCAT ATTCTAATCT TCCCTGTTAT
    AACAAAGTCA TTAAAAAGGA GCCAGGTAGT GATCCACTTG TAGATTCTTT CCAGAGAGCA
    AATAGTGTCC ATTCACATAG CCCAGGAGTT AATCACTCAT TACAGGCCAG TGATCTGCCC
    ATCTCATACA AAGCTAATGG TGCTCTATCA TCATCAGGGA GGACTAATGC AGAAAGTCCG
    TGCAGTATGT TTATGCCCAA TGATAAAAAT GGTCTAGAAA AGAAAGATTA TTTTGGAGTC
    CATTCGAATG CCCCTGGTCT GAAGGACAAG CAGTGGCCTC CCTATGGAAC TGATGTTTCA
    GTGAGACAAC ATGATTCACT TGACTCGCAG TCTCCAGGAA AAGTGTGGAG CTCATGCAAG
    CTGAGTGACA GTTCCGCTGC CCTGCCCTCC TCTGCAAGTA CACAGGATAA GAACTGGAAT
    GGTAGACAGG TCAGTCTTAA TCAGGGGATG AAGGAGTCTG CGCTGTTCCA AGAAAAACTC
    TGGAATTCAG TTGCTGCCAG TGACAGGTGC AGTGCCACGC CCAGTGACAG AAGTAGCATC
    ACCCCCTGTT CTGAGCTTCA GGACAAGAAC TGGGGATCAT TTCCTAATCC AACAGTAAAC
    TCTTTGAAGA CTGACTCCAG CCAGAACCAC TGGGATCCCT ATAGCCTAGA TGATAATATG
    GATGATGGCC AATCAAAGAG TGTAAAAGAG GAGGATGATG AGGAGATCTG GTCTGATAGT
    GAGCACAACT TTCTGGATGA GAATATCGGA GGTGTGGCTG TAGCCCCAGG ACATGGCTCT
    ATCCTAATTG AATGTGCAAG GAGAGAGCTC CATGCTACCA CTCCACTGAA AAAGCCCAAC
    AGATGTCATC CAACTCGAAT CTCTCTTGTA TTCTACCAAC ACAAGAACTT AAACCAACCT
    AACCATGGAT TGGCACTTTG GGAGGCCAAA ATGAAGCAGT TGGCAGAAAG GGCACGTGCA
    AGGGAGGAAG AGGCTGCCAA GCTAGGTATC AAACAGGAAG TAAAAAGTTT GGGTAAAAAG
    AGGAAGTGGG GTGGTGCTGC CACTACAGAA ACCCCACCAG TTGAGAAGAA AGACTATACC
    CCTACCCGAC AGGCAGCTAC AATTTTAACA GATTCTGCTA CCACTTCATT CTCCTATGCC
    TATACCAAGG TTACGGGACC ATACAGTCGC TTTATATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53115
    Clone ID Related Accession (Same CDS sequence) XM_031897483.1 , XM_031897482.1 , XM_031897485.1
    Accession Version XM_031897482.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5799bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product methylcytosine dioxygenase tet3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGGGGATC TCCCGTACGT TGGCTGTACC ATGATGGATA CTCAGCCCGC GCCAGTTCCA 
    CATGTGCTGC CCCAAGATGT ATATGAATTT CCTGATGACC AAGAGTCCCT TGGGAGATTA
    AGAGTCTCAG AGATGCCAGC GGAGCTGAAT GGTGGTGGTG GTGGTGGCAG TGCAGCTGCC
    TTTGCCATGG AACTTCCAGA GCAATCTAAT AAAAAAAGGA AGCGTTGTGG GGTCTGTGTG
    CCATGTCTAA GGAAGGAACC ATGTGGAGCC TGTTACAACT GTGTGAACCG GAGTACCAGC
    CACCAGATAT GCAAGATGCG AAAATGCGAG CAGCTCAAAA AGAAAAGAGT GGTCCCCATG
    AAAGGAGTGG AGACTGTAAA TAACGACGGC TCCAAGAATC AGGCAAAAGA ACAAGTGCCC
    AGTGTGAAGA ACTGTTCTGA AAGCATTTTA GTTGATGGAC CCAAAACAGA CCAAATGGAA
    GCAGGGCCAG TTAACCATGT TCAAGAAGGA AGGTTAAAGC AGGAATGTGA CTCTACCCTC
    CCAAGTAAGG GATGTGAGGA TCTAGCAAAC CAATTATTAA TGGAAGCAAA TTCGTGGCTA
    AGCAATACAG CTGCTCCTCA GGATCCCTGC AACAAGCTCA ACTGGGATAA ACCCACCATT
    CCCAACCATG CTGCTAATAA CAATTCAAAC CTAGAAGATG CCAAAAATCT AGTTGCATTC
    TCTGCTGTGG CTGAGGCTAT GTCTACCTAT GGCATGCCTG CATCAGGGAC CCCCTCTTCG
    GTATCACTGC AGCTATATGA AAAGTTTAAT TATGAGACAA ACAGGGACAA CTCTGGGCAC
    TTGGAAGGCA ATGCTCCATC ATGTCCCGAA GACCTAAACA CTCTAAAAGC AGCCCTTGCA
    CTTGCAAAGC ATGGCGTGAA ACCACCTAAC TGTAACTGTG ACGGCCCTGA GTGCCCGGAC
    TATCTAGAAT GGCTGGAAAA TAAAATAAAA TCTACAGTTA AAGGTTCTCA GGAGAGCCCA
    TTCCCTAATC TGGGTCAGGT CAGTAAAGAG CTGGTGCAAA AACAATACCC TAAAGAACAG
    GTGCTAAATC TAGAAAATAA AAATTCAACA TGTCCTTCAG GTAACCTTCC CTTTTCTCAA
    AATGCACTGA GTCTTGCTAA AGAAAAAAAT ATAAGTCTGC AAACGGCCAT TGCCATTGAA
    GCTCTTACAC AACTATCATC TGCACTTCCA CAGACAAACA ATGAATGCCC CAATGCTCCT
    TCCCAACCTT TAATAAACCC TCATGATCAG TTAACTCATT TCCCCTCTGC TAAAGGCAAC
    CAGTTACCAA TGTTACCAGT GGCCCGCAAT GAACTGTTCC AGAATCAGCA GTCCCAATTA
    TACACTGGCA AAAATGCTCT TCCTGTGCCC CAATCTCCAA GGCAAACTTC TTGGGAGCAG
    AACAAGAAAT CTAGTTACCA AGAAGGACAA TACATTCCTG AAAATTTATC ACATAGTTCT
    TCTGTGTTGC CCAGTGATGC TTCTACCCCA CAAAAACCTG AATTCCTTCA GCAGTGGGTC
    CAAAATGCAG ATCTTTTGAA GTCTCCATCT GATCCTATGA CTGGGTTAAA GCAATTGCTT
    GGGAACACAG ATGAGTACAT CAAGTCTGTG TTTAAAGGGC CAGAGGCTTT GCCCAACAAA
    AAAAATGTGA AGCCCAAACA TACCATTAAA TCCATAAAAA AAGAATCCAC CGAGTTTCTC
    AAAATGTCAC CAGATCAACA GCTCTCTCAG CTTCTTCAAA CAAATGAATT TCATCGTAAT
    ACCCAGGCTG CACTTCAGCA GCATCTCCAC CATAAACGCA ATCTATTTGT GGACCCAAAT
    GCTATGGAAG CATGTACCCA AGAACAGCAA AACTGGTGGG TGCCGAGTTC TCAACAAGCT
    CCAGTTTCCA AGACAACAGA GAAACCTGTA AAAGAGAGGA AGAAAAGGCG ACAGTCTCCT
    TCACAAAAGC AAGTGGAACC AAAACCGAAA CCTCAACGTA AGCAAGTGCA AATAAAGAAA
    CCCAAGGTGA AGGAAGGAAG TGCAGTCTTC ATGCCTGTGA GCCAAATAAG CCTGGATACT
    TTTCGCAGAG TTGAAAAAGA AGAGAACCAG GGGAAAGAAA TGGATGCAGA GAACTCTCTT
    CCTAATAACG TACAAACAGA ACTACTTGAA TCCCAGTCTT TACAACTGAC TGGATCACAG
    GCAAATCCTG ATGACAGAAA GACTGTAAAC ACTCAGGAAA TGTGCAATGA AAATCAGTCT
    AACATTGGGA AGGCCAATAA CTTTGCACTT TGTGTCAATC GGGCTAATTC TTTTGTTGCA
    AAAGACCAAT GTCCAACACC TTCCACACAT GATACCAGCT CTAGCTCTGG ACAAGGTGAT
    TCTGCCAACC AGCACCACAC AAATGTGAGT GATGTTCCTG GGCAAAATGA CCTTAGCTGC
    CTTGATGACA AGTTGGAAGA TCTAATAAGG CAGTTTGAAG CTGAGTTTGG GGAAGATTTC
    TCTCTACCTG GTTCTGCGGT CCCCTCTCAG AATGGTGAAG GACCGCCAAA ACAAACACCA
    TCAGGAGATC CACAATTTAA ACTGCCCTTT CCTTCCCAGT TGTTACCACC TGAGAATTCC
    ACAAAGCCTG CCACCCATTC CAACCCTGCA CTTTCAAATA ATCCTGTATC ACGTGAAGTT
    TCTAATAACC TTGATAGTCT GTTCTCATCA AAGTCTCCCA AGCAGATAAA AATCGAATCT
    TCTGGTGCTA TAACAGTGGT GTCTACCACA TGCTTCTACT CAGAGGAAAA CCAACATCTT
    GATGGAACTC CTACAAAAAG TGACCTGCCT TTCAATCCTA CTCTTAGTGG GTTTTTGGAC
    TCGCCTCTCA AATATCTGAC ATCTCCAACC AAGAGCTTAA TAGATACACC AGCTAAAAAG
    GCTCAAGCAG AATTTCCAAC ATGCGACTGT GTTGAACAAA TTAATGAAAA AGATGAAGGC
    CCATATTATA CACATCTGGG TTCTGGACCA ACAGTGGCTT CTATCAGAGA GCTAATGGAA
    GAGCGGTTTG GACAAAAAGG GGATGCAATC CGCATTGAAA AGGTGATCTA CACAGGAAAA
    GAAGGAAAAA GCTCAAGGGG TTGTCCTATT GCAAAATGGG TCATTCGCCG TCAGAGCGAG
    GATGAGAAGT TGATGTGCCT TGTCCGGCAA CGGGCAGGCC ACCATTGTGA AAATGCAGTC
    ATCATTATCT TAATTATGGC CTGGGAAGGA ATTCCTCGCT CTCTAGGAGA TTCTCTCTAC
    AACGACATCA CTGAGACAAT CACCAAGTAT GGAAATCCAA CATCACGGAG ATGTGGACTG
    AATGATGATC GAACCTGTGC ATGCCAAGGC AAGGACCCAA ATACCTGTGG TGCTTCTTTC
    TCTTTTGGGT GCTCATGGAG CATGTACTTT AATGGTTGCA AGTATGCTCG TAGCAAGACC
    CCCCGGAAGT TCCGACTTAT AGGAGAAAAT CCCAAGGAGG AAGATGGCCT GAAGGATAAC
    TTTCAGAATC TAGCTACAAA GGTTGCTCCA GTGTACAAGA TGCTTGCTCC TCAGGCTTAT
    CAAAATCAGG TTAACAATGA GGATATTGCA ATAGATTGTC GGCTAGGTCT CAAGGAGGGG
    CGTCCTTTCT CTGGGGTGAC TGCATGCATG GATTTCTGTG CCCATGCCCA CAAAGACCAG
    CACAATCTAT ACAATGGATG CACTGTGGTC TGCACTCTAA CTAAGGAGGA TAACCGTATG
    ATTGGCAGGG TTGCTGAGGA TGAGCAGCTC CATGTACTTC CACTGTACAA GGTTTCCACA
    ACTGATGAAT TTGGGAGTGA GGAGGGTCAA CTTGAAAAGA TAAAGAAAGG AGGAATCCAC
    GTGTTGAGCT CTTTTCCCCG GGAGGTACGC AAGCTTTCAG AACCAGCAAA GTCTTGCAGG
    CAGAGACAAC TAGAAGCCAA GAAGGCCGCA GCCGAAAAGA AGAAACTTCA AAAGGAGAAA
    CTGGTCTCTC CGGATAAGAC CAAGCAGGAG CCATCAGACA AAAAGACGTG CCAGCAAAAC
    CCAGGTGTTC CTCAGCAACA GACAAAGCCT TGTATAAAAG TGGAGCCTTC CAATCACTAC
    AATAACTTTA AGTACAATGG AAATGGAGTT GTGGAGAGCT ACTCAGTGTT GGGCAGCTGC
    AGGCCGTCCG ACCCTTACAG CATGAACAGC GTTTACTCCT ATCATTCTTT CTATGCACAA
    CCTAACCTGC CTTCCGTGAA TGGCTTTCAT TCAAAATATG CTCTTCCTCC CTTTGGATTT
    TATGGCTTTC CTAACAATCC TGTGGTTCCC AACCAGTTCA TGAATTATGG TACAAGTGAT
    GCAAGGAACA GTGGATGGAT GAATAATTGT TTTGAGAAAA AGCCAGAGCT TCAGTCACTG
    GCAGATGGAA TGAATCAAAG TTACGGGAGT GAGTTATCAG AGCAGAGTTT TAGGAGATCA
    AGTGAGGTAC CACACCACTA CAGCCTCCAA AATCCCAGCA GTCAAAAATC AGTTAATGTT
    CCCCACCGAA CTACTCCTGC ACCAGTGGAG ACTACTCCAT ATTCTAATCT TCCCTGTTAT
    AACAAAGTCA TTAAAAAGGA GCCAGGTAGT GATCCACTTG TAGATTCTTT CCAGAGAGCA
    AATAGTGTCC ATTCACATAG CCCAGGAGTT AATCACTCAT TACAGGCCAG TGATCTGCCC
    ATCTCATACA AAGCTAATGG TGCTCTATCA TCATCAGGGA GGACTAATGC AGAAAGTCCG
    TGCAGTATGT TTATGCCCAA TGATAAAAAT GGTCTAGAAA AGAAAGATTA TTTTGGAGTC
    CATTCGAATG CCCCTGGTCT GAAGGACAAG CAGTGGCCTC CCTATGGAAC TGATGTTTCA
    GTGAGACAAC ATGATTCACT TGACTCGCAG TCTCCAGGAA AAGTGTGGAG CTCATGCAAG
    CTGAGTGACA GTTCCGCTGC CCTGCCCTCC TCTGCAAGTA CACAGGATAA GAACTGGAAT
    GGTAGACAGG TCAGTCTTAA TCAGGGGATG AAGGAGTCTG CGCTGTTCCA AGAAAAACTC
    TGGAATTCAG TTGCTGCCAG TGACAGGTGC AGTGCCACGC CCAGTGACAG AAGTAGCATC
    ACCCCCTGTT CTGAGCTTCA GGACAAGAAC TGGGGATCAT TTCCTAATCC AACAGTAAAC
    TCTTTGAAGA CTGACTCCAG CCAGAACCAC TGGGATCCCT ATAGCCTAGA TGATAATATG
    GATGATGGCC AATCAAAGAG TGTAAAAGAG GAGGATGATG AGGAGATCTG GTCTGATAGT
    GAGCACAACT TTCTGGATGA GAATATCGGA GGTGTGGCTG TAGCCCCAGG ACATGGCTCT
    ATCCTAATTG AATGTGCAAG GAGAGAGCTC CATGCTACCA CTCCACTGAA AAAGCCCAAC
    AGATGTCATC CAACTCGAAT CTCTCTTGTA TTCTACCAAC ACAAGAACTT AAACCAACCT
    AACCATGGAT TGGCACTTTG GGAGGCCAAA ATGAAGCAGT TGGCAGAAAG GGCACGTGCA
    AGGGAGGAAG AGGCTGCCAA GCTAGGTATC AAACAGGAAG TAAAAAGTTT GGGTAAAAAG
    AGGAAGTGGG GTGGTGCTGC CACTACAGAA ACCCCACCAG TTGAGAAGAA AGACTATACC
    CCTACCCGAC AGGCAGCTAC AATTTTAACA GATTCTGCTA CCACTTCATT CTCCTATGCC
    TATACCAAGG TTACGGGACC ATACAGTCGC TTTATATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753342.1
    CDS570..6368
    Translation

    Target ORF information:

    RefSeq Version XM_031897482.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis tet methylcytosine dioxygenase 3 (tet3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897482.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGGGGATC TCCCGTACGT TGGCTGTACC ATGATGGATA CTCAGCCCGC GCCAGTTCCA 
    CATGTGCTGC CCCAAGATGT ATATGAATTT CCTGATGACC AAGAGTCCCT TGGGAGATTA
    AGAGTCTCAG AGATGCCAGC GGAGCTGAAT GGTGGTGGTG GTGGTGGCAG TGCAGCTGCC
    TTTGCCATGG AACTTCCAGA GCAATCTAAT AAAAAAAGGA AGCGTTGTGG GGTCTGTGTG
    CCATGTCTAA GGAAGGAACC ATGTGGAGCC TGTTACAACT GTGTGAACCG GAGTACCAGC
    CACCAGATAT GCAAGATGCG AAAATGCGAG CAGCTCAAAA AGAAAAGAGT GGTCCCCATG
    AAAGGAGTGG AGACTGTAAA TAACGACGGC TCCAAGAATC AGGCAAAAGA ACAAGTGCCC
    AGTGTGAAGA ACTGTTCTGA AAGCATTTTA GTTGATGGAC CCAAAACAGA CCAAATGGAA
    GCAGGGCCAG TTAACCATGT TCAAGAAGGA AGGTTAAAGC AGGAATGTGA CTCTACCCTC
    CCAAGTAAGG GATGTGAGGA TCTAGCAAAC CAATTATTAA TGGAAGCAAA TTCGTGGCTA
    AGCAATACAG CTGCTCCTCA GGATCCCTGC AACAAGCTCA ACTGGGATAA ACCCACCATT
    CCCAACCATG CTGCTAATAA CAATTCAAAC CTAGAAGATG CCAAAAATCT AGTTGCATTC
    TCTGCTGTGG CTGAGGCTAT GTCTACCTAT GGCATGCCTG CATCAGGGAC CCCCTCTTCG
    GTATCACTGC AGCTATATGA AAAGTTTAAT TATGAGACAA ACAGGGACAA CTCTGGGCAC
    TTGGAAGGCA ATGCTCCATC ATGTCCCGAA GACCTAAACA CTCTAAAAGC AGCCCTTGCA
    CTTGCAAAGC ATGGCGTGAA ACCACCTAAC TGTAACTGTG ACGGCCCTGA GTGCCCGGAC
    TATCTAGAAT GGCTGGAAAA TAAAATAAAA TCTACAGTTA AAGGTTCTCA GGAGAGCCCA
    TTCCCTAATC TGGGTCAGGT CAGTAAAGAG CTGGTGCAAA AACAATACCC TAAAGAACAG
    GTGCTAAATC TAGAAAATAA AAATTCAACA TGTCCTTCAG GTAACCTTCC CTTTTCTCAA
    AATGCACTGA GTCTTGCTAA AGAAAAAAAT ATAAGTCTGC AAACGGCCAT TGCCATTGAA
    GCTCTTACAC AACTATCATC TGCACTTCCA CAGACAAACA ATGAATGCCC CAATGCTCCT
    TCCCAACCTT TAATAAACCC TCATGATCAG TTAACTCATT TCCCCTCTGC TAAAGGCAAC
    CAGTTACCAA TGTTACCAGT GGCCCGCAAT GAACTGTTCC AGAATCAGCA GTCCCAATTA
    TACACTGGCA AAAATGCTCT TCCTGTGCCC CAATCTCCAA GGCAAACTTC TTGGGAGCAG
    AACAAGAAAT CTAGTTACCA AGAAGGACAA TACATTCCTG AAAATTTATC ACATAGTTCT
    TCTGTGTTGC CCAGTGATGC TTCTACCCCA CAAAAACCTG AATTCCTTCA GCAGTGGGTC
    CAAAATGCAG ATCTTTTGAA GTCTCCATCT GATCCTATGA CTGGGTTAAA GCAATTGCTT
    GGGAACACAG ATGAGTACAT CAAGTCTGTG TTTAAAGGGC CAGAGGCTTT GCCCAACAAA
    AAAAATGTGA AGCCCAAACA TACCATTAAA TCCATAAAAA AAGAATCCAC CGAGTTTCTC
    AAAATGTCAC CAGATCAACA GCTCTCTCAG CTTCTTCAAA CAAATGAATT TCATCGTAAT
    ACCCAGGCTG CACTTCAGCA GCATCTCCAC CATAAACGCA ATCTATTTGT GGACCCAAAT
    GCTATGGAAG CATGTACCCA AGAACAGCAA AACTGGTGGG TGCCGAGTTC TCAACAAGCT
    CCAGTTTCCA AGACAACAGA GAAACCTGTA AAAGAGAGGA AGAAAAGGCG ACAGTCTCCT
    TCACAAAAGC AAGTGGAACC AAAACCGAAA CCTCAACGTA AGCAAGTGCA AATAAAGAAA
    CCCAAGGTGA AGGAAGGAAG TGCAGTCTTC ATGCCTGTGA GCCAAATAAG CCTGGATACT
    TTTCGCAGAG TTGAAAAAGA AGAGAACCAG GGGAAAGAAA TGGATGCAGA GAACTCTCTT
    CCTAATAACG TACAAACAGA ACTACTTGAA TCCCAGTCTT TACAACTGAC TGGATCACAG
    GCAAATCCTG ATGACAGAAA GACTGTAAAC ACTCAGGAAA TGTGCAATGA AAATCAGTCT
    AACATTGGGA AGGCCAATAA CTTTGCACTT TGTGTCAATC GGGCTAATTC TTTTGTTGCA
    AAAGACCAAT GTCCAACACC TTCCACACAT GATACCAGCT CTAGCTCTGG ACAAGGTGAT
    TCTGCCAACC AGCACCACAC AAATGTGAGT GATGTTCCTG GGCAAAATGA CCTTAGCTGC
    CTTGATGACA AGTTGGAAGA TCTAATAAGG CAGTTTGAAG CTGAGTTTGG GGAAGATTTC
    TCTCTACCTG GTTCTGCGGT CCCCTCTCAG AATGGTGAAG GACCGCCAAA ACAAACACCA
    TCAGGAGATC CACAATTTAA ACTGCCCTTT CCTTCCCAGT TGTTACCACC TGAGAATTCC
    ACAAAGCCTG CCACCCATTC CAACCCTGCA CTTTCAAATA ATCCTGTATC ACGTGAAGTT
    TCTAATAACC TTGATAGTCT GTTCTCATCA AAGTCTCCCA AGCAGATAAA AATCGAATCT
    TCTGGTGCTA TAACAGTGGT GTCTACCACA TGCTTCTACT CAGAGGAAAA CCAACATCTT
    GATGGAACTC CTACAAAAAG TGACCTGCCT TTCAATCCTA CTCTTAGTGG GTTTTTGGAC
    TCGCCTCTCA AATATCTGAC ATCTCCAACC AAGAGCTTAA TAGATACACC AGCTAAAAAG
    GCTCAAGCAG AATTTCCAAC ATGCGACTGT GTTGAACAAA TTAATGAAAA AGATGAAGGC
    CCATATTATA CACATCTGGG TTCTGGACCA ACAGTGGCTT CTATCAGAGA GCTAATGGAA
    GAGCGGTTTG GACAAAAAGG GGATGCAATC CGCATTGAAA AGGTGATCTA CACAGGAAAA
    GAAGGAAAAA GCTCAAGGGG TTGTCCTATT GCAAAATGGG TCATTCGCCG TCAGAGCGAG
    GATGAGAAGT TGATGTGCCT TGTCCGGCAA CGGGCAGGCC ACCATTGTGA AAATGCAGTC
    ATCATTATCT TAATTATGGC CTGGGAAGGA ATTCCTCGCT CTCTAGGAGA TTCTCTCTAC
    AACGACATCA CTGAGACAAT CACCAAGTAT GGAAATCCAA CATCACGGAG ATGTGGACTG
    AATGATGATC GAACCTGTGC ATGCCAAGGC AAGGACCCAA ATACCTGTGG TGCTTCTTTC
    TCTTTTGGGT GCTCATGGAG CATGTACTTT AATGGTTGCA AGTATGCTCG TAGCAAGACC
    CCCCGGAAGT TCCGACTTAT AGGAGAAAAT CCCAAGGAGG AAGATGGCCT GAAGGATAAC
    TTTCAGAATC TAGCTACAAA GGTTGCTCCA GTGTACAAGA TGCTTGCTCC TCAGGCTTAT
    CAAAATCAGG TTAACAATGA GGATATTGCA ATAGATTGTC GGCTAGGTCT CAAGGAGGGG
    CGTCCTTTCT CTGGGGTGAC TGCATGCATG GATTTCTGTG CCCATGCCCA CAAAGACCAG
    CACAATCTAT ACAATGGATG CACTGTGGTC TGCACTCTAA CTAAGGAGGA TAACCGTATG
    ATTGGCAGGG TTGCTGAGGA TGAGCAGCTC CATGTACTTC CACTGTACAA GGTTTCCACA
    ACTGATGAAT TTGGGAGTGA GGAGGGTCAA CTTGAAAAGA TAAAGAAAGG AGGAATCCAC
    GTGTTGAGCT CTTTTCCCCG GGAGGTACGC AAGCTTTCAG AACCAGCAAA GTCTTGCAGG
    CAGAGACAAC TAGAAGCCAA GAAGGCCGCA GCCGAAAAGA AGAAACTTCA AAAGGAGAAA
    CTGGTCTCTC CGGATAAGAC CAAGCAGGAG CCATCAGACA AAAAGACGTG CCAGCAAAAC
    CCAGGTGTTC CTCAGCAACA GACAAAGCCT TGTATAAAAG TGGAGCCTTC CAATCACTAC
    AATAACTTTA AGTACAATGG AAATGGAGTT GTGGAGAGCT ACTCAGTGTT GGGCAGCTGC
    AGGCCGTCCG ACCCTTACAG CATGAACAGC GTTTACTCCT ATCATTCTTT CTATGCACAA
    CCTAACCTGC CTTCCGTGAA TGGCTTTCAT TCAAAATATG CTCTTCCTCC CTTTGGATTT
    TATGGCTTTC CTAACAATCC TGTGGTTCCC AACCAGTTCA TGAATTATGG TACAAGTGAT
    GCAAGGAACA GTGGATGGAT GAATAATTGT TTTGAGAAAA AGCCAGAGCT TCAGTCACTG
    GCAGATGGAA TGAATCAAAG TTACGGGAGT GAGTTATCAG AGCAGAGTTT TAGGAGATCA
    AGTGAGGTAC CACACCACTA CAGCCTCCAA AATCCCAGCA GTCAAAAATC AGTTAATGTT
    CCCCACCGAA CTACTCCTGC ACCAGTGGAG ACTACTCCAT ATTCTAATCT TCCCTGTTAT
    AACAAAGTCA TTAAAAAGGA GCCAGGTAGT GATCCACTTG TAGATTCTTT CCAGAGAGCA
    AATAGTGTCC ATTCACATAG CCCAGGAGTT AATCACTCAT TACAGGCCAG TGATCTGCCC
    ATCTCATACA AAGCTAATGG TGCTCTATCA TCATCAGGGA GGACTAATGC AGAAAGTCCG
    TGCAGTATGT TTATGCCCAA TGATAAAAAT GGTCTAGAAA AGAAAGATTA TTTTGGAGTC
    CATTCGAATG CCCCTGGTCT GAAGGACAAG CAGTGGCCTC CCTATGGAAC TGATGTTTCA
    GTGAGACAAC ATGATTCACT TGACTCGCAG TCTCCAGGAA AAGTGTGGAG CTCATGCAAG
    CTGAGTGACA GTTCCGCTGC CCTGCCCTCC TCTGCAAGTA CACAGGATAA GAACTGGAAT
    GGTAGACAGG TCAGTCTTAA TCAGGGGATG AAGGAGTCTG CGCTGTTCCA AGAAAAACTC
    TGGAATTCAG TTGCTGCCAG TGACAGGTGC AGTGCCACGC CCAGTGACAG AAGTAGCATC
    ACCCCCTGTT CTGAGCTTCA GGACAAGAAC TGGGGATCAT TTCCTAATCC AACAGTAAAC
    TCTTTGAAGA CTGACTCCAG CCAGAACCAC TGGGATCCCT ATAGCCTAGA TGATAATATG
    GATGATGGCC AATCAAAGAG TGTAAAAGAG GAGGATGATG AGGAGATCTG GTCTGATAGT
    GAGCACAACT TTCTGGATGA GAATATCGGA GGTGTGGCTG TAGCCCCAGG ACATGGCTCT
    ATCCTAATTG AATGTGCAAG GAGAGAGCTC CATGCTACCA CTCCACTGAA AAAGCCCAAC
    AGATGTCATC CAACTCGAAT CTCTCTTGTA TTCTACCAAC ACAAGAACTT AAACCAACCT
    AACCATGGAT TGGCACTTTG GGAGGCCAAA ATGAAGCAGT TGGCAGAAAG GGCACGTGCA
    AGGGAGGAAG AGGCTGCCAA GCTAGGTATC AAACAGGAAG TAAAAAGTTT GGGTAAAAAG
    AGGAAGTGGG GTGGTGCTGC CACTACAGAA ACCCCACCAG TTGAGAAGAA AGACTATACC
    CCTACCCGAC AGGCAGCTAC AATTTTAACA GATTCTGCTA CCACTTCATT CTCCTATGCC
    TATACCAAGG TTACGGGACC ATACAGTCGC TTTATATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53115
    Clone ID Related Accession (Same CDS sequence) XM_031897483.1 , XM_031897482.1 , XM_031897485.1
    Accession Version XM_031897485.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5799bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product methylcytosine dioxygenase tet3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGGGGATC TCCCGTACGT TGGCTGTACC ATGATGGATA CTCAGCCCGC GCCAGTTCCA 
    CATGTGCTGC CCCAAGATGT ATATGAATTT CCTGATGACC AAGAGTCCCT TGGGAGATTA
    AGAGTCTCAG AGATGCCAGC GGAGCTGAAT GGTGGTGGTG GTGGTGGCAG TGCAGCTGCC
    TTTGCCATGG AACTTCCAGA GCAATCTAAT AAAAAAAGGA AGCGTTGTGG GGTCTGTGTG
    CCATGTCTAA GGAAGGAACC ATGTGGAGCC TGTTACAACT GTGTGAACCG GAGTACCAGC
    CACCAGATAT GCAAGATGCG AAAATGCGAG CAGCTCAAAA AGAAAAGAGT GGTCCCCATG
    AAAGGAGTGG AGACTGTAAA TAACGACGGC TCCAAGAATC AGGCAAAAGA ACAAGTGCCC
    AGTGTGAAGA ACTGTTCTGA AAGCATTTTA GTTGATGGAC CCAAAACAGA CCAAATGGAA
    GCAGGGCCAG TTAACCATGT TCAAGAAGGA AGGTTAAAGC AGGAATGTGA CTCTACCCTC
    CCAAGTAAGG GATGTGAGGA TCTAGCAAAC CAATTATTAA TGGAAGCAAA TTCGTGGCTA
    AGCAATACAG CTGCTCCTCA GGATCCCTGC AACAAGCTCA ACTGGGATAA ACCCACCATT
    CCCAACCATG CTGCTAATAA CAATTCAAAC CTAGAAGATG CCAAAAATCT AGTTGCATTC
    TCTGCTGTGG CTGAGGCTAT GTCTACCTAT GGCATGCCTG CATCAGGGAC CCCCTCTTCG
    GTATCACTGC AGCTATATGA AAAGTTTAAT TATGAGACAA ACAGGGACAA CTCTGGGCAC
    TTGGAAGGCA ATGCTCCATC ATGTCCCGAA GACCTAAACA CTCTAAAAGC AGCCCTTGCA
    CTTGCAAAGC ATGGCGTGAA ACCACCTAAC TGTAACTGTG ACGGCCCTGA GTGCCCGGAC
    TATCTAGAAT GGCTGGAAAA TAAAATAAAA TCTACAGTTA AAGGTTCTCA GGAGAGCCCA
    TTCCCTAATC TGGGTCAGGT CAGTAAAGAG CTGGTGCAAA AACAATACCC TAAAGAACAG
    GTGCTAAATC TAGAAAATAA AAATTCAACA TGTCCTTCAG GTAACCTTCC CTTTTCTCAA
    AATGCACTGA GTCTTGCTAA AGAAAAAAAT ATAAGTCTGC AAACGGCCAT TGCCATTGAA
    GCTCTTACAC AACTATCATC TGCACTTCCA CAGACAAACA ATGAATGCCC CAATGCTCCT
    TCCCAACCTT TAATAAACCC TCATGATCAG TTAACTCATT TCCCCTCTGC TAAAGGCAAC
    CAGTTACCAA TGTTACCAGT GGCCCGCAAT GAACTGTTCC AGAATCAGCA GTCCCAATTA
    TACACTGGCA AAAATGCTCT TCCTGTGCCC CAATCTCCAA GGCAAACTTC TTGGGAGCAG
    AACAAGAAAT CTAGTTACCA AGAAGGACAA TACATTCCTG AAAATTTATC ACATAGTTCT
    TCTGTGTTGC CCAGTGATGC TTCTACCCCA CAAAAACCTG AATTCCTTCA GCAGTGGGTC
    CAAAATGCAG ATCTTTTGAA GTCTCCATCT GATCCTATGA CTGGGTTAAA GCAATTGCTT
    GGGAACACAG ATGAGTACAT CAAGTCTGTG TTTAAAGGGC CAGAGGCTTT GCCCAACAAA
    AAAAATGTGA AGCCCAAACA TACCATTAAA TCCATAAAAA AAGAATCCAC CGAGTTTCTC
    AAAATGTCAC CAGATCAACA GCTCTCTCAG CTTCTTCAAA CAAATGAATT TCATCGTAAT
    ACCCAGGCTG CACTTCAGCA GCATCTCCAC CATAAACGCA ATCTATTTGT GGACCCAAAT
    GCTATGGAAG CATGTACCCA AGAACAGCAA AACTGGTGGG TGCCGAGTTC TCAACAAGCT
    CCAGTTTCCA AGACAACAGA GAAACCTGTA AAAGAGAGGA AGAAAAGGCG ACAGTCTCCT
    TCACAAAAGC AAGTGGAACC AAAACCGAAA CCTCAACGTA AGCAAGTGCA AATAAAGAAA
    CCCAAGGTGA AGGAAGGAAG TGCAGTCTTC ATGCCTGTGA GCCAAATAAG CCTGGATACT
    TTTCGCAGAG TTGAAAAAGA AGAGAACCAG GGGAAAGAAA TGGATGCAGA GAACTCTCTT
    CCTAATAACG TACAAACAGA ACTACTTGAA TCCCAGTCTT TACAACTGAC TGGATCACAG
    GCAAATCCTG ATGACAGAAA GACTGTAAAC ACTCAGGAAA TGTGCAATGA AAATCAGTCT
    AACATTGGGA AGGCCAATAA CTTTGCACTT TGTGTCAATC GGGCTAATTC TTTTGTTGCA
    AAAGACCAAT GTCCAACACC TTCCACACAT GATACCAGCT CTAGCTCTGG ACAAGGTGAT
    TCTGCCAACC AGCACCACAC AAATGTGAGT GATGTTCCTG GGCAAAATGA CCTTAGCTGC
    CTTGATGACA AGTTGGAAGA TCTAATAAGG CAGTTTGAAG CTGAGTTTGG GGAAGATTTC
    TCTCTACCTG GTTCTGCGGT CCCCTCTCAG AATGGTGAAG GACCGCCAAA ACAAACACCA
    TCAGGAGATC CACAATTTAA ACTGCCCTTT CCTTCCCAGT TGTTACCACC TGAGAATTCC
    ACAAAGCCTG CCACCCATTC CAACCCTGCA CTTTCAAATA ATCCTGTATC ACGTGAAGTT
    TCTAATAACC TTGATAGTCT GTTCTCATCA AAGTCTCCCA AGCAGATAAA AATCGAATCT
    TCTGGTGCTA TAACAGTGGT GTCTACCACA TGCTTCTACT CAGAGGAAAA CCAACATCTT
    GATGGAACTC CTACAAAAAG TGACCTGCCT TTCAATCCTA CTCTTAGTGG GTTTTTGGAC
    TCGCCTCTCA AATATCTGAC ATCTCCAACC AAGAGCTTAA TAGATACACC AGCTAAAAAG
    GCTCAAGCAG AATTTCCAAC ATGCGACTGT GTTGAACAAA TTAATGAAAA AGATGAAGGC
    CCATATTATA CACATCTGGG TTCTGGACCA ACAGTGGCTT CTATCAGAGA GCTAATGGAA
    GAGCGGTTTG GACAAAAAGG GGATGCAATC CGCATTGAAA AGGTGATCTA CACAGGAAAA
    GAAGGAAAAA GCTCAAGGGG TTGTCCTATT GCAAAATGGG TCATTCGCCG TCAGAGCGAG
    GATGAGAAGT TGATGTGCCT TGTCCGGCAA CGGGCAGGCC ACCATTGTGA AAATGCAGTC
    ATCATTATCT TAATTATGGC CTGGGAAGGA ATTCCTCGCT CTCTAGGAGA TTCTCTCTAC
    AACGACATCA CTGAGACAAT CACCAAGTAT GGAAATCCAA CATCACGGAG ATGTGGACTG
    AATGATGATC GAACCTGTGC ATGCCAAGGC AAGGACCCAA ATACCTGTGG TGCTTCTTTC
    TCTTTTGGGT GCTCATGGAG CATGTACTTT AATGGTTGCA AGTATGCTCG TAGCAAGACC
    CCCCGGAAGT TCCGACTTAT AGGAGAAAAT CCCAAGGAGG AAGATGGCCT GAAGGATAAC
    TTTCAGAATC TAGCTACAAA GGTTGCTCCA GTGTACAAGA TGCTTGCTCC TCAGGCTTAT
    CAAAATCAGG TTAACAATGA GGATATTGCA ATAGATTGTC GGCTAGGTCT CAAGGAGGGG
    CGTCCTTTCT CTGGGGTGAC TGCATGCATG GATTTCTGTG CCCATGCCCA CAAAGACCAG
    CACAATCTAT ACAATGGATG CACTGTGGTC TGCACTCTAA CTAAGGAGGA TAACCGTATG
    ATTGGCAGGG TTGCTGAGGA TGAGCAGCTC CATGTACTTC CACTGTACAA GGTTTCCACA
    ACTGATGAAT TTGGGAGTGA GGAGGGTCAA CTTGAAAAGA TAAAGAAAGG AGGAATCCAC
    GTGTTGAGCT CTTTTCCCCG GGAGGTACGC AAGCTTTCAG AACCAGCAAA GTCTTGCAGG
    CAGAGACAAC TAGAAGCCAA GAAGGCCGCA GCCGAAAAGA AGAAACTTCA AAAGGAGAAA
    CTGGTCTCTC CGGATAAGAC CAAGCAGGAG CCATCAGACA AAAAGACGTG CCAGCAAAAC
    CCAGGTGTTC CTCAGCAACA GACAAAGCCT TGTATAAAAG TGGAGCCTTC CAATCACTAC
    AATAACTTTA AGTACAATGG AAATGGAGTT GTGGAGAGCT ACTCAGTGTT GGGCAGCTGC
    AGGCCGTCCG ACCCTTACAG CATGAACAGC GTTTACTCCT ATCATTCTTT CTATGCACAA
    CCTAACCTGC CTTCCGTGAA TGGCTTTCAT TCAAAATATG CTCTTCCTCC CTTTGGATTT
    TATGGCTTTC CTAACAATCC TGTGGTTCCC AACCAGTTCA TGAATTATGG TACAAGTGAT
    GCAAGGAACA GTGGATGGAT GAATAATTGT TTTGAGAAAA AGCCAGAGCT TCAGTCACTG
    GCAGATGGAA TGAATCAAAG TTACGGGAGT GAGTTATCAG AGCAGAGTTT TAGGAGATCA
    AGTGAGGTAC CACACCACTA CAGCCTCCAA AATCCCAGCA GTCAAAAATC AGTTAATGTT
    CCCCACCGAA CTACTCCTGC ACCAGTGGAG ACTACTCCAT ATTCTAATCT TCCCTGTTAT
    AACAAAGTCA TTAAAAAGGA GCCAGGTAGT GATCCACTTG TAGATTCTTT CCAGAGAGCA
    AATAGTGTCC ATTCACATAG CCCAGGAGTT AATCACTCAT TACAGGCCAG TGATCTGCCC
    ATCTCATACA AAGCTAATGG TGCTCTATCA TCATCAGGGA GGACTAATGC AGAAAGTCCG
    TGCAGTATGT TTATGCCCAA TGATAAAAAT GGTCTAGAAA AGAAAGATTA TTTTGGAGTC
    CATTCGAATG CCCCTGGTCT GAAGGACAAG CAGTGGCCTC CCTATGGAAC TGATGTTTCA
    GTGAGACAAC ATGATTCACT TGACTCGCAG TCTCCAGGAA AAGTGTGGAG CTCATGCAAG
    CTGAGTGACA GTTCCGCTGC CCTGCCCTCC TCTGCAAGTA CACAGGATAA GAACTGGAAT
    GGTAGACAGG TCAGTCTTAA TCAGGGGATG AAGGAGTCTG CGCTGTTCCA AGAAAAACTC
    TGGAATTCAG TTGCTGCCAG TGACAGGTGC AGTGCCACGC CCAGTGACAG AAGTAGCATC
    ACCCCCTGTT CTGAGCTTCA GGACAAGAAC TGGGGATCAT TTCCTAATCC AACAGTAAAC
    TCTTTGAAGA CTGACTCCAG CCAGAACCAC TGGGATCCCT ATAGCCTAGA TGATAATATG
    GATGATGGCC AATCAAAGAG TGTAAAAGAG GAGGATGATG AGGAGATCTG GTCTGATAGT
    GAGCACAACT TTCTGGATGA GAATATCGGA GGTGTGGCTG TAGCCCCAGG ACATGGCTCT
    ATCCTAATTG AATGTGCAAG GAGAGAGCTC CATGCTACCA CTCCACTGAA AAAGCCCAAC
    AGATGTCATC CAACTCGAAT CTCTCTTGTA TTCTACCAAC ACAAGAACTT AAACCAACCT
    AACCATGGAT TGGCACTTTG GGAGGCCAAA ATGAAGCAGT TGGCAGAAAG GGCACGTGCA
    AGGGAGGAAG AGGCTGCCAA GCTAGGTATC AAACAGGAAG TAAAAAGTTT GGGTAAAAAG
    AGGAAGTGGG GTGGTGCTGC CACTACAGAA ACCCCACCAG TTGAGAAGAA AGACTATACC
    CCTACCCGAC AGGCAGCTAC AATTTTAACA GATTCTGCTA CCACTTCATT CTCCTATGCC
    TATACCAAGG TTACGGGACC ATACAGTCGC TTTATATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753345.1
    CDS446..6244
    Translation

    Target ORF information:

    RefSeq Version XM_031897485.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis tet methylcytosine dioxygenase 3 (tet3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897485.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGGGGATC TCCCGTACGT TGGCTGTACC ATGATGGATA CTCAGCCCGC GCCAGTTCCA 
    CATGTGCTGC CCCAAGATGT ATATGAATTT CCTGATGACC AAGAGTCCCT TGGGAGATTA
    AGAGTCTCAG AGATGCCAGC GGAGCTGAAT GGTGGTGGTG GTGGTGGCAG TGCAGCTGCC
    TTTGCCATGG AACTTCCAGA GCAATCTAAT AAAAAAAGGA AGCGTTGTGG GGTCTGTGTG
    CCATGTCTAA GGAAGGAACC ATGTGGAGCC TGTTACAACT GTGTGAACCG GAGTACCAGC
    CACCAGATAT GCAAGATGCG AAAATGCGAG CAGCTCAAAA AGAAAAGAGT GGTCCCCATG
    AAAGGAGTGG AGACTGTAAA TAACGACGGC TCCAAGAATC AGGCAAAAGA ACAAGTGCCC
    AGTGTGAAGA ACTGTTCTGA AAGCATTTTA GTTGATGGAC CCAAAACAGA CCAAATGGAA
    GCAGGGCCAG TTAACCATGT TCAAGAAGGA AGGTTAAAGC AGGAATGTGA CTCTACCCTC
    CCAAGTAAGG GATGTGAGGA TCTAGCAAAC CAATTATTAA TGGAAGCAAA TTCGTGGCTA
    AGCAATACAG CTGCTCCTCA GGATCCCTGC AACAAGCTCA ACTGGGATAA ACCCACCATT
    CCCAACCATG CTGCTAATAA CAATTCAAAC CTAGAAGATG CCAAAAATCT AGTTGCATTC
    TCTGCTGTGG CTGAGGCTAT GTCTACCTAT GGCATGCCTG CATCAGGGAC CCCCTCTTCG
    GTATCACTGC AGCTATATGA AAAGTTTAAT TATGAGACAA ACAGGGACAA CTCTGGGCAC
    TTGGAAGGCA ATGCTCCATC ATGTCCCGAA GACCTAAACA CTCTAAAAGC AGCCCTTGCA
    CTTGCAAAGC ATGGCGTGAA ACCACCTAAC TGTAACTGTG ACGGCCCTGA GTGCCCGGAC
    TATCTAGAAT GGCTGGAAAA TAAAATAAAA TCTACAGTTA AAGGTTCTCA GGAGAGCCCA
    TTCCCTAATC TGGGTCAGGT CAGTAAAGAG CTGGTGCAAA AACAATACCC TAAAGAACAG
    GTGCTAAATC TAGAAAATAA AAATTCAACA TGTCCTTCAG GTAACCTTCC CTTTTCTCAA
    AATGCACTGA GTCTTGCTAA AGAAAAAAAT ATAAGTCTGC AAACGGCCAT TGCCATTGAA
    GCTCTTACAC AACTATCATC TGCACTTCCA CAGACAAACA ATGAATGCCC CAATGCTCCT
    TCCCAACCTT TAATAAACCC TCATGATCAG TTAACTCATT TCCCCTCTGC TAAAGGCAAC
    CAGTTACCAA TGTTACCAGT GGCCCGCAAT GAACTGTTCC AGAATCAGCA GTCCCAATTA
    TACACTGGCA AAAATGCTCT TCCTGTGCCC CAATCTCCAA GGCAAACTTC TTGGGAGCAG
    AACAAGAAAT CTAGTTACCA AGAAGGACAA TACATTCCTG AAAATTTATC ACATAGTTCT
    TCTGTGTTGC CCAGTGATGC TTCTACCCCA CAAAAACCTG AATTCCTTCA GCAGTGGGTC
    CAAAATGCAG ATCTTTTGAA GTCTCCATCT GATCCTATGA CTGGGTTAAA GCAATTGCTT
    GGGAACACAG ATGAGTACAT CAAGTCTGTG TTTAAAGGGC CAGAGGCTTT GCCCAACAAA
    AAAAATGTGA AGCCCAAACA TACCATTAAA TCCATAAAAA AAGAATCCAC CGAGTTTCTC
    AAAATGTCAC CAGATCAACA GCTCTCTCAG CTTCTTCAAA CAAATGAATT TCATCGTAAT
    ACCCAGGCTG CACTTCAGCA GCATCTCCAC CATAAACGCA ATCTATTTGT GGACCCAAAT
    GCTATGGAAG CATGTACCCA AGAACAGCAA AACTGGTGGG TGCCGAGTTC TCAACAAGCT
    CCAGTTTCCA AGACAACAGA GAAACCTGTA AAAGAGAGGA AGAAAAGGCG ACAGTCTCCT
    TCACAAAAGC AAGTGGAACC AAAACCGAAA CCTCAACGTA AGCAAGTGCA AATAAAGAAA
    CCCAAGGTGA AGGAAGGAAG TGCAGTCTTC ATGCCTGTGA GCCAAATAAG CCTGGATACT
    TTTCGCAGAG TTGAAAAAGA AGAGAACCAG GGGAAAGAAA TGGATGCAGA GAACTCTCTT
    CCTAATAACG TACAAACAGA ACTACTTGAA TCCCAGTCTT TACAACTGAC TGGATCACAG
    GCAAATCCTG ATGACAGAAA GACTGTAAAC ACTCAGGAAA TGTGCAATGA AAATCAGTCT
    AACATTGGGA AGGCCAATAA CTTTGCACTT TGTGTCAATC GGGCTAATTC TTTTGTTGCA
    AAAGACCAAT GTCCAACACC TTCCACACAT GATACCAGCT CTAGCTCTGG ACAAGGTGAT
    TCTGCCAACC AGCACCACAC AAATGTGAGT GATGTTCCTG GGCAAAATGA CCTTAGCTGC
    CTTGATGACA AGTTGGAAGA TCTAATAAGG CAGTTTGAAG CTGAGTTTGG GGAAGATTTC
    TCTCTACCTG GTTCTGCGGT CCCCTCTCAG AATGGTGAAG GACCGCCAAA ACAAACACCA
    TCAGGAGATC CACAATTTAA ACTGCCCTTT CCTTCCCAGT TGTTACCACC TGAGAATTCC
    ACAAAGCCTG CCACCCATTC CAACCCTGCA CTTTCAAATA ATCCTGTATC ACGTGAAGTT
    TCTAATAACC TTGATAGTCT GTTCTCATCA AAGTCTCCCA AGCAGATAAA AATCGAATCT
    TCTGGTGCTA TAACAGTGGT GTCTACCACA TGCTTCTACT CAGAGGAAAA CCAACATCTT
    GATGGAACTC CTACAAAAAG TGACCTGCCT TTCAATCCTA CTCTTAGTGG GTTTTTGGAC
    TCGCCTCTCA AATATCTGAC ATCTCCAACC AAGAGCTTAA TAGATACACC AGCTAAAAAG
    GCTCAAGCAG AATTTCCAAC ATGCGACTGT GTTGAACAAA TTAATGAAAA AGATGAAGGC
    CCATATTATA CACATCTGGG TTCTGGACCA ACAGTGGCTT CTATCAGAGA GCTAATGGAA
    GAGCGGTTTG GACAAAAAGG GGATGCAATC CGCATTGAAA AGGTGATCTA CACAGGAAAA
    GAAGGAAAAA GCTCAAGGGG TTGTCCTATT GCAAAATGGG TCATTCGCCG TCAGAGCGAG
    GATGAGAAGT TGATGTGCCT TGTCCGGCAA CGGGCAGGCC ACCATTGTGA AAATGCAGTC
    ATCATTATCT TAATTATGGC CTGGGAAGGA ATTCCTCGCT CTCTAGGAGA TTCTCTCTAC
    AACGACATCA CTGAGACAAT CACCAAGTAT GGAAATCCAA CATCACGGAG ATGTGGACTG
    AATGATGATC GAACCTGTGC ATGCCAAGGC AAGGACCCAA ATACCTGTGG TGCTTCTTTC
    TCTTTTGGGT GCTCATGGAG CATGTACTTT AATGGTTGCA AGTATGCTCG TAGCAAGACC
    CCCCGGAAGT TCCGACTTAT AGGAGAAAAT CCCAAGGAGG AAGATGGCCT GAAGGATAAC
    TTTCAGAATC TAGCTACAAA GGTTGCTCCA GTGTACAAGA TGCTTGCTCC TCAGGCTTAT
    CAAAATCAGG TTAACAATGA GGATATTGCA ATAGATTGTC GGCTAGGTCT CAAGGAGGGG
    CGTCCTTTCT CTGGGGTGAC TGCATGCATG GATTTCTGTG CCCATGCCCA CAAAGACCAG
    CACAATCTAT ACAATGGATG CACTGTGGTC TGCACTCTAA CTAAGGAGGA TAACCGTATG
    ATTGGCAGGG TTGCTGAGGA TGAGCAGCTC CATGTACTTC CACTGTACAA GGTTTCCACA
    ACTGATGAAT TTGGGAGTGA GGAGGGTCAA CTTGAAAAGA TAAAGAAAGG AGGAATCCAC
    GTGTTGAGCT CTTTTCCCCG GGAGGTACGC AAGCTTTCAG AACCAGCAAA GTCTTGCAGG
    CAGAGACAAC TAGAAGCCAA GAAGGCCGCA GCCGAAAAGA AGAAACTTCA AAAGGAGAAA
    CTGGTCTCTC CGGATAAGAC CAAGCAGGAG CCATCAGACA AAAAGACGTG CCAGCAAAAC
    CCAGGTGTTC CTCAGCAACA GACAAAGCCT TGTATAAAAG TGGAGCCTTC CAATCACTAC
    AATAACTTTA AGTACAATGG AAATGGAGTT GTGGAGAGCT ACTCAGTGTT GGGCAGCTGC
    AGGCCGTCCG ACCCTTACAG CATGAACAGC GTTTACTCCT ATCATTCTTT CTATGCACAA
    CCTAACCTGC CTTCCGTGAA TGGCTTTCAT TCAAAATATG CTCTTCCTCC CTTTGGATTT
    TATGGCTTTC CTAACAATCC TGTGGTTCCC AACCAGTTCA TGAATTATGG TACAAGTGAT
    GCAAGGAACA GTGGATGGAT GAATAATTGT TTTGAGAAAA AGCCAGAGCT TCAGTCACTG
    GCAGATGGAA TGAATCAAAG TTACGGGAGT GAGTTATCAG AGCAGAGTTT TAGGAGATCA
    AGTGAGGTAC CACACCACTA CAGCCTCCAA AATCCCAGCA GTCAAAAATC AGTTAATGTT
    CCCCACCGAA CTACTCCTGC ACCAGTGGAG ACTACTCCAT ATTCTAATCT TCCCTGTTAT
    AACAAAGTCA TTAAAAAGGA GCCAGGTAGT GATCCACTTG TAGATTCTTT CCAGAGAGCA
    AATAGTGTCC ATTCACATAG CCCAGGAGTT AATCACTCAT TACAGGCCAG TGATCTGCCC
    ATCTCATACA AAGCTAATGG TGCTCTATCA TCATCAGGGA GGACTAATGC AGAAAGTCCG
    TGCAGTATGT TTATGCCCAA TGATAAAAAT GGTCTAGAAA AGAAAGATTA TTTTGGAGTC
    CATTCGAATG CCCCTGGTCT GAAGGACAAG CAGTGGCCTC CCTATGGAAC TGATGTTTCA
    GTGAGACAAC ATGATTCACT TGACTCGCAG TCTCCAGGAA AAGTGTGGAG CTCATGCAAG
    CTGAGTGACA GTTCCGCTGC CCTGCCCTCC TCTGCAAGTA CACAGGATAA GAACTGGAAT
    GGTAGACAGG TCAGTCTTAA TCAGGGGATG AAGGAGTCTG CGCTGTTCCA AGAAAAACTC
    TGGAATTCAG TTGCTGCCAG TGACAGGTGC AGTGCCACGC CCAGTGACAG AAGTAGCATC
    ACCCCCTGTT CTGAGCTTCA GGACAAGAAC TGGGGATCAT TTCCTAATCC AACAGTAAAC
    TCTTTGAAGA CTGACTCCAG CCAGAACCAC TGGGATCCCT ATAGCCTAGA TGATAATATG
    GATGATGGCC AATCAAAGAG TGTAAAAGAG GAGGATGATG AGGAGATCTG GTCTGATAGT
    GAGCACAACT TTCTGGATGA GAATATCGGA GGTGTGGCTG TAGCCCCAGG ACATGGCTCT
    ATCCTAATTG AATGTGCAAG GAGAGAGCTC CATGCTACCA CTCCACTGAA AAAGCCCAAC
    AGATGTCATC CAACTCGAAT CTCTCTTGTA TTCTACCAAC ACAAGAACTT AAACCAACCT
    AACCATGGAT TGGCACTTTG GGAGGCCAAA ATGAAGCAGT TGGCAGAAAG GGCACGTGCA
    AGGGAGGAAG AGGCTGCCAA GCTAGGTATC AAACAGGAAG TAAAAAGTTT GGGTAAAAAG
    AGGAAGTGGG GTGGTGCTGC CACTACAGAA ACCCCACCAG TTGAGAAGAA AGACTATACC
    CCTACCCGAC AGGCAGCTAC AATTTTAACA GATTCTGCTA CCACTTCATT CTCCTATGCC
    TATACCAAGG TTACGGGACC ATACAGTCGC TTTATATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53116
    Clone ID Related Accession (Same CDS sequence) XM_031897486.1
    Accession Version XM_031897486.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5742bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product methylcytosine dioxygenase tet3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGGGGATC TCCCGTACGT TGGCTGTACC ATGATGGATA CTCAGCCCGC GCCAGTTCCA 
    CATGTGCTGC CCCAAGATGT ATATGAATTT CCTGATGACC AAGAGTCCCT TGGGAGATTA
    AGAGTCTCAG AGATGCCAGC GGAGCTGAAT GGTGGTGGTG GTGGTGGCAG TGCAGCTGCC
    TTTGCCATGG AACTTCCAGA GCAATCTAAT AAAAAAAGGA AGCGTTGTGG GGTCTGTGTG
    CCATGTCTAA GGAAGGAACC ATGTGGAGCC TGTTACAACT GTGTGAACCG GAGTACCAGC
    CACCAGATAT GCAAGATGCG AAAATGCGAG CAGCTCAAAA AGAAAAGAGT GGTCCCCATG
    AAAGGAGTGG AGAACTGTTC TGAAAGCATT TTAGTTGATG GACCCAAAAC AGACCAAATG
    GAAGCAGGGC CAGTTAACCA TGTTCAAGAA GGAAGGTTAA AGCAGGAATG TGACTCTACC
    CTCCCAAGTA AGGGATGTGA GGATCTAGCA AACCAATTAT TAATGGAAGC AAATTCGTGG
    CTAAGCAATA CAGCTGCTCC TCAGGATCCC TGCAACAAGC TCAACTGGGA TAAACCCACC
    ATTCCCAACC ATGCTGCTAA TAACAATTCA AACCTAGAAG ATGCCAAAAA TCTAGTTGCA
    TTCTCTGCTG TGGCTGAGGC TATGTCTACC TATGGCATGC CTGCATCAGG GACCCCCTCT
    TCGGTATCAC TGCAGCTATA TGAAAAGTTT AATTATGAGA CAAACAGGGA CAACTCTGGG
    CACTTGGAAG GCAATGCTCC ATCATGTCCC GAAGACCTAA ACACTCTAAA AGCAGCCCTT
    GCACTTGCAA AGCATGGCGT GAAACCACCT AACTGTAACT GTGACGGCCC TGAGTGCCCG
    GACTATCTAG AATGGCTGGA AAATAAAATA AAATCTACAG TTAAAGGTTC TCAGGAGAGC
    CCATTCCCTA ATCTGGGTCA GGTCAGTAAA GAGCTGGTGC AAAAACAATA CCCTAAAGAA
    CAGGTGCTAA ATCTAGAAAA TAAAAATTCA ACATGTCCTT CAGGTAACCT TCCCTTTTCT
    CAAAATGCAC TGAGTCTTGC TAAAGAAAAA AATATAAGTC TGCAAACGGC CATTGCCATT
    GAAGCTCTTA CACAACTATC ATCTGCACTT CCACAGACAA ACAATGAATG CCCCAATGCT
    CCTTCCCAAC CTTTAATAAA CCCTCATGAT CAGTTAACTC ATTTCCCCTC TGCTAAAGGC
    AACCAGTTAC CAATGTTACC AGTGGCCCGC AATGAACTGT TCCAGAATCA GCAGTCCCAA
    TTATACACTG GCAAAAATGC TCTTCCTGTG CCCCAATCTC CAAGGCAAAC TTCTTGGGAG
    CAGAACAAGA AATCTAGTTA CCAAGAAGGA CAATACATTC CTGAAAATTT ATCACATAGT
    TCTTCTGTGT TGCCCAGTGA TGCTTCTACC CCACAAAAAC CTGAATTCCT TCAGCAGTGG
    GTCCAAAATG CAGATCTTTT GAAGTCTCCA TCTGATCCTA TGACTGGGTT AAAGCAATTG
    CTTGGGAACA CAGATGAGTA CATCAAGTCT GTGTTTAAAG GGCCAGAGGC TTTGCCCAAC
    AAAAAAAATG TGAAGCCCAA ACATACCATT AAATCCATAA AAAAAGAATC CACCGAGTTT
    CTCAAAATGT CACCAGATCA ACAGCTCTCT CAGCTTCTTC AAACAAATGA ATTTCATCGT
    AATACCCAGG CTGCACTTCA GCAGCATCTC CACCATAAAC GCAATCTATT TGTGGACCCA
    AATGCTATGG AAGCATGTAC CCAAGAACAG CAAAACTGGT GGGTGCCGAG TTCTCAACAA
    GCTCCAGTTT CCAAGACAAC AGAGAAACCT GTAAAAGAGA GGAAGAAAAG GCGACAGTCT
    CCTTCACAAA AGCAAGTGGA ACCAAAACCG AAACCTCAAC GTAAGCAAGT GCAAATAAAG
    AAACCCAAGG TGAAGGAAGG AAGTGCAGTC TTCATGCCTG TGAGCCAAAT AAGCCTGGAT
    ACTTTTCGCA GAGTTGAAAA AGAAGAGAAC CAGGGGAAAG AAATGGATGC AGAGAACTCT
    CTTCCTAATA ACGTACAAAC AGAACTACTT GAATCCCAGT CTTTACAACT GACTGGATCA
    CAGGCAAATC CTGATGACAG AAAGACTGTA AACACTCAGG AAATGTGCAA TGAAAATCAG
    TCTAACATTG GGAAGGCCAA TAACTTTGCA CTTTGTGTCA ATCGGGCTAA TTCTTTTGTT
    GCAAAAGACC AATGTCCAAC ACCTTCCACA CATGATACCA GCTCTAGCTC TGGACAAGGT
    GATTCTGCCA ACCAGCACCA CACAAATGTG AGTGATGTTC CTGGGCAAAA TGACCTTAGC
    TGCCTTGATG ACAAGTTGGA AGATCTAATA AGGCAGTTTG AAGCTGAGTT TGGGGAAGAT
    TTCTCTCTAC CTGGTTCTGC GGTCCCCTCT CAGAATGGTG AAGGACCGCC AAAACAAACA
    CCATCAGGAG ATCCACAATT TAAACTGCCC TTTCCTTCCC AGTTGTTACC ACCTGAGAAT
    TCCACAAAGC CTGCCACCCA TTCCAACCCT GCACTTTCAA ATAATCCTGT ATCACGTGAA
    GTTTCTAATA ACCTTGATAG TCTGTTCTCA TCAAAGTCTC CCAAGCAGAT AAAAATCGAA
    TCTTCTGGTG CTATAACAGT GGTGTCTACC ACATGCTTCT ACTCAGAGGA AAACCAACAT
    CTTGATGGAA CTCCTACAAA AAGTGACCTG CCTTTCAATC CTACTCTTAG TGGGTTTTTG
    GACTCGCCTC TCAAATATCT GACATCTCCA ACCAAGAGCT TAATAGATAC ACCAGCTAAA
    AAGGCTCAAG CAGAATTTCC AACATGCGAC TGTGTTGAAC AAATTAATGA AAAAGATGAA
    GGCCCATATT ATACACATCT GGGTTCTGGA CCAACAGTGG CTTCTATCAG AGAGCTAATG
    GAAGAGCGGT TTGGACAAAA AGGGGATGCA ATCCGCATTG AAAAGGTGAT CTACACAGGA
    AAAGAAGGAA AAAGCTCAAG GGGTTGTCCT ATTGCAAAAT GGGTCATTCG CCGTCAGAGC
    GAGGATGAGA AGTTGATGTG CCTTGTCCGG CAACGGGCAG GCCACCATTG TGAAAATGCA
    GTCATCATTA TCTTAATTAT GGCCTGGGAA GGAATTCCTC GCTCTCTAGG AGATTCTCTC
    TACAACGACA TCACTGAGAC AATCACCAAG TATGGAAATC CAACATCACG GAGATGTGGA
    CTGAATGATG ATCGAACCTG TGCATGCCAA GGCAAGGACC CAAATACCTG TGGTGCTTCT
    TTCTCTTTTG GGTGCTCATG GAGCATGTAC TTTAATGGTT GCAAGTATGC TCGTAGCAAG
    ACCCCCCGGA AGTTCCGACT TATAGGAGAA AATCCCAAGG AGGAAGATGG CCTGAAGGAT
    AACTTTCAGA ATCTAGCTAC AAAGGTTGCT CCAGTGTACA AGATGCTTGC TCCTCAGGCT
    TATCAAAATC AGGTTAACAA TGAGGATATT GCAATAGATT GTCGGCTAGG TCTCAAGGAG
    GGGCGTCCTT TCTCTGGGGT GACTGCATGC ATGGATTTCT GTGCCCATGC CCACAAAGAC
    CAGCACAATC TATACAATGG ATGCACTGTG GTCTGCACTC TAACTAAGGA GGATAACCGT
    ATGATTGGCA GGGTTGCTGA GGATGAGCAG CTCCATGTAC TTCCACTGTA CAAGGTTTCC
    ACAACTGATG AATTTGGGAG TGAGGAGGGT CAACTTGAAA AGATAAAGAA AGGAGGAATC
    CACGTGTTGA GCTCTTTTCC CCGGGAGGTA CGCAAGCTTT CAGAACCAGC AAAGTCTTGC
    AGGCAGAGAC AACTAGAAGC CAAGAAGGCC GCAGCCGAAA AGAAGAAACT TCAAAAGGAG
    AAACTGGTCT CTCCGGATAA GACCAAGCAG GAGCCATCAG ACAAAAAGAC GTGCCAGCAA
    AACCCAGGTG TTCCTCAGCA ACAGACAAAG CCTTGTATAA AAGTGGAGCC TTCCAATCAC
    TACAATAACT TTAAGTACAA TGGAAATGGA GTTGTGGAGA GCTACTCAGT GTTGGGCAGC
    TGCAGGCCGT CCGACCCTTA CAGCATGAAC AGCGTTTACT CCTATCATTC TTTCTATGCA
    CAACCTAACC TGCCTTCCGT GAATGGCTTT CATTCAAAAT ATGCTCTTCC TCCCTTTGGA
    TTTTATGGCT TTCCTAACAA TCCTGTGGTT CCCAACCAGT TCATGAATTA TGGTACAAGT
    GATGCAAGGA ACAGTGGATG GATGAATAAT TGTTTTGAGA AAAAGCCAGA GCTTCAGTCA
    CTGGCAGATG GAATGAATCA AAGTTACGGG AGTGAGTTAT CAGAGCAGAG TTTTAGGAGA
    TCAAGTGAGG TACCACACCA CTACAGCCTC CAAAATCCCA GCAGTCAAAA ATCAGTTAAT
    GTTCCCCACC GAACTACTCC TGCACCAGTG GAGACTACTC CATATTCTAA TCTTCCCTGT
    TATAACAAAG TCATTAAAAA GGAGCCAGGT AGTGATCCAC TTGTAGATTC TTTCCAGAGA
    GCAAATAGTG TCCATTCACA TAGCCCAGGA GTTAATCACT CATTACAGGC CAGTGATCTG
    CCCATCTCAT ACAAAGCTAA TGGTGCTCTA TCATCATCAG GGAGGACTAA TGCAGAAAGT
    CCGTGCAGTA TGTTTATGCC CAATGATAAA AATGGTCTAG AAAAGAAAGA TTATTTTGGA
    GTCCATTCGA ATGCCCCTGG TCTGAAGGAC AAGCAGTGGC CTCCCTATGG AACTGATGTT
    TCAGTGAGAC AACATGATTC ACTTGACTCG CAGTCTCCAG GAAAAGTGTG GAGCTCATGC
    AAGCTGAGTG ACAGTTCCGC TGCCCTGCCC TCCTCTGCAA GTACACAGGA TAAGAACTGG
    AATGGTAGAC AGGTCAGTCT TAATCAGGGG ATGAAGGAGT CTGCGCTGTT CCAAGAAAAA
    CTCTGGAATT CAGTTGCTGC CAGTGACAGG TGCAGTGCCA CGCCCAGTGA CAGAAGTAGC
    ATCACCCCCT GTTCTGAGCT TCAGGACAAG AACTGGGGAT CATTTCCTAA TCCAACAGTA
    AACTCTTTGA AGACTGACTC CAGCCAGAAC CACTGGGATC CCTATAGCCT AGATGATAAT
    ATGGATGATG GCCAATCAAA GAGTGTAAAA GAGGAGGATG ATGAGGAGAT CTGGTCTGAT
    AGTGAGCACA ACTTTCTGGA TGAGAATATC GGAGGTGTGG CTGTAGCCCC AGGACATGGC
    TCTATCCTAA TTGAATGTGC AAGGAGAGAG CTCCATGCTA CCACTCCACT GAAAAAGCCC
    AACAGATGTC ATCCAACTCG AATCTCTCTT GTATTCTACC AACACAAGAA CTTAAACCAA
    CCTAACCATG GATTGGCACT TTGGGAGGCC AAAATGAAGC AGTTGGCAGA AAGGGCACGT
    GCAAGGGAGG AAGAGGCTGC CAAGCTAGGT ATCAAACAGG AAGTAAAAAG TTTGGGTAAA
    AAGAGGAAGT GGGGTGGTGC TGCCACTACA GAAACCCCAC CAGTTGAGAA GAAAGACTAT
    ACCCCTACCC GACAGGCAGC TACAATTTTA ACAGATTCTG CTACCACTTC ATTCTCCTAT
    GCCTATACCA AGGTTACGGG ACCATACAGT CGCTTTATAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753346.1
    CDS672..6413
    Translation

    Target ORF information:

    RefSeq Version XM_031897486.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis tet methylcytosine dioxygenase 3 (tet3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897486.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGGGGATC TCCCGTACGT TGGCTGTACC ATGATGGATA CTCAGCCCGC GCCAGTTCCA 
    CATGTGCTGC CCCAAGATGT ATATGAATTT CCTGATGACC AAGAGTCCCT TGGGAGATTA
    AGAGTCTCAG AGATGCCAGC GGAGCTGAAT GGTGGTGGTG GTGGTGGCAG TGCAGCTGCC
    TTTGCCATGG AACTTCCAGA GCAATCTAAT AAAAAAAGGA AGCGTTGTGG GGTCTGTGTG
    CCATGTCTAA GGAAGGAACC ATGTGGAGCC TGTTACAACT GTGTGAACCG GAGTACCAGC
    CACCAGATAT GCAAGATGCG AAAATGCGAG CAGCTCAAAA AGAAAAGAGT GGTCCCCATG
    AAAGGAGTGG AGAACTGTTC TGAAAGCATT TTAGTTGATG GACCCAAAAC AGACCAAATG
    GAAGCAGGGC CAGTTAACCA TGTTCAAGAA GGAAGGTTAA AGCAGGAATG TGACTCTACC
    CTCCCAAGTA AGGGATGTGA GGATCTAGCA AACCAATTAT TAATGGAAGC AAATTCGTGG
    CTAAGCAATA CAGCTGCTCC TCAGGATCCC TGCAACAAGC TCAACTGGGA TAAACCCACC
    ATTCCCAACC ATGCTGCTAA TAACAATTCA AACCTAGAAG ATGCCAAAAA TCTAGTTGCA
    TTCTCTGCTG TGGCTGAGGC TATGTCTACC TATGGCATGC CTGCATCAGG GACCCCCTCT
    TCGGTATCAC TGCAGCTATA TGAAAAGTTT AATTATGAGA CAAACAGGGA CAACTCTGGG
    CACTTGGAAG GCAATGCTCC ATCATGTCCC GAAGACCTAA ACACTCTAAA AGCAGCCCTT
    GCACTTGCAA AGCATGGCGT GAAACCACCT AACTGTAACT GTGACGGCCC TGAGTGCCCG
    GACTATCTAG AATGGCTGGA AAATAAAATA AAATCTACAG TTAAAGGTTC TCAGGAGAGC
    CCATTCCCTA ATCTGGGTCA GGTCAGTAAA GAGCTGGTGC AAAAACAATA CCCTAAAGAA
    CAGGTGCTAA ATCTAGAAAA TAAAAATTCA ACATGTCCTT CAGGTAACCT TCCCTTTTCT
    CAAAATGCAC TGAGTCTTGC TAAAGAAAAA AATATAAGTC TGCAAACGGC CATTGCCATT
    GAAGCTCTTA CACAACTATC ATCTGCACTT CCACAGACAA ACAATGAATG CCCCAATGCT
    CCTTCCCAAC CTTTAATAAA CCCTCATGAT CAGTTAACTC ATTTCCCCTC TGCTAAAGGC
    AACCAGTTAC CAATGTTACC AGTGGCCCGC AATGAACTGT TCCAGAATCA GCAGTCCCAA
    TTATACACTG GCAAAAATGC TCTTCCTGTG CCCCAATCTC CAAGGCAAAC TTCTTGGGAG
    CAGAACAAGA AATCTAGTTA CCAAGAAGGA CAATACATTC CTGAAAATTT ATCACATAGT
    TCTTCTGTGT TGCCCAGTGA TGCTTCTACC CCACAAAAAC CTGAATTCCT TCAGCAGTGG
    GTCCAAAATG CAGATCTTTT GAAGTCTCCA TCTGATCCTA TGACTGGGTT AAAGCAATTG
    CTTGGGAACA CAGATGAGTA CATCAAGTCT GTGTTTAAAG GGCCAGAGGC TTTGCCCAAC
    AAAAAAAATG TGAAGCCCAA ACATACCATT AAATCCATAA AAAAAGAATC CACCGAGTTT
    CTCAAAATGT CACCAGATCA ACAGCTCTCT CAGCTTCTTC AAACAAATGA ATTTCATCGT
    AATACCCAGG CTGCACTTCA GCAGCATCTC CACCATAAAC GCAATCTATT TGTGGACCCA
    AATGCTATGG AAGCATGTAC CCAAGAACAG CAAAACTGGT GGGTGCCGAG TTCTCAACAA
    GCTCCAGTTT CCAAGACAAC AGAGAAACCT GTAAAAGAGA GGAAGAAAAG GCGACAGTCT
    CCTTCACAAA AGCAAGTGGA ACCAAAACCG AAACCTCAAC GTAAGCAAGT GCAAATAAAG
    AAACCCAAGG TGAAGGAAGG AAGTGCAGTC TTCATGCCTG TGAGCCAAAT AAGCCTGGAT
    ACTTTTCGCA GAGTTGAAAA AGAAGAGAAC CAGGGGAAAG AAATGGATGC AGAGAACTCT
    CTTCCTAATA ACGTACAAAC AGAACTACTT GAATCCCAGT CTTTACAACT GACTGGATCA
    CAGGCAAATC CTGATGACAG AAAGACTGTA AACACTCAGG AAATGTGCAA TGAAAATCAG
    TCTAACATTG GGAAGGCCAA TAACTTTGCA CTTTGTGTCA ATCGGGCTAA TTCTTTTGTT
    GCAAAAGACC AATGTCCAAC ACCTTCCACA CATGATACCA GCTCTAGCTC TGGACAAGGT
    GATTCTGCCA ACCAGCACCA CACAAATGTG AGTGATGTTC CTGGGCAAAA TGACCTTAGC
    TGCCTTGATG ACAAGTTGGA AGATCTAATA AGGCAGTTTG AAGCTGAGTT TGGGGAAGAT
    TTCTCTCTAC CTGGTTCTGC GGTCCCCTCT CAGAATGGTG AAGGACCGCC AAAACAAACA
    CCATCAGGAG ATCCACAATT TAAACTGCCC TTTCCTTCCC AGTTGTTACC ACCTGAGAAT
    TCCACAAAGC CTGCCACCCA TTCCAACCCT GCACTTTCAA ATAATCCTGT ATCACGTGAA
    GTTTCTAATA ACCTTGATAG TCTGTTCTCA TCAAAGTCTC CCAAGCAGAT AAAAATCGAA
    TCTTCTGGTG CTATAACAGT GGTGTCTACC ACATGCTTCT ACTCAGAGGA AAACCAACAT
    CTTGATGGAA CTCCTACAAA AAGTGACCTG CCTTTCAATC CTACTCTTAG TGGGTTTTTG
    GACTCGCCTC TCAAATATCT GACATCTCCA ACCAAGAGCT TAATAGATAC ACCAGCTAAA
    AAGGCTCAAG CAGAATTTCC AACATGCGAC TGTGTTGAAC AAATTAATGA AAAAGATGAA
    GGCCCATATT ATACACATCT GGGTTCTGGA CCAACAGTGG CTTCTATCAG AGAGCTAATG
    GAAGAGCGGT TTGGACAAAA AGGGGATGCA ATCCGCATTG AAAAGGTGAT CTACACAGGA
    AAAGAAGGAA AAAGCTCAAG GGGTTGTCCT ATTGCAAAAT GGGTCATTCG CCGTCAGAGC
    GAGGATGAGA AGTTGATGTG CCTTGTCCGG CAACGGGCAG GCCACCATTG TGAAAATGCA
    GTCATCATTA TCTTAATTAT GGCCTGGGAA GGAATTCCTC GCTCTCTAGG AGATTCTCTC
    TACAACGACA TCACTGAGAC AATCACCAAG TATGGAAATC CAACATCACG GAGATGTGGA
    CTGAATGATG ATCGAACCTG TGCATGCCAA GGCAAGGACC CAAATACCTG TGGTGCTTCT
    TTCTCTTTTG GGTGCTCATG GAGCATGTAC TTTAATGGTT GCAAGTATGC TCGTAGCAAG
    ACCCCCCGGA AGTTCCGACT TATAGGAGAA AATCCCAAGG AGGAAGATGG CCTGAAGGAT
    AACTTTCAGA ATCTAGCTAC AAAGGTTGCT CCAGTGTACA AGATGCTTGC TCCTCAGGCT
    TATCAAAATC AGGTTAACAA TGAGGATATT GCAATAGATT GTCGGCTAGG TCTCAAGGAG
    GGGCGTCCTT TCTCTGGGGT GACTGCATGC ATGGATTTCT GTGCCCATGC CCACAAAGAC
    CAGCACAATC TATACAATGG ATGCACTGTG GTCTGCACTC TAACTAAGGA GGATAACCGT
    ATGATTGGCA GGGTTGCTGA GGATGAGCAG CTCCATGTAC TTCCACTGTA CAAGGTTTCC
    ACAACTGATG AATTTGGGAG TGAGGAGGGT CAACTTGAAA AGATAAAGAA AGGAGGAATC
    CACGTGTTGA GCTCTTTTCC CCGGGAGGTA CGCAAGCTTT CAGAACCAGC AAAGTCTTGC
    AGGCAGAGAC AACTAGAAGC CAAGAAGGCC GCAGCCGAAA AGAAGAAACT TCAAAAGGAG
    AAACTGGTCT CTCCGGATAA GACCAAGCAG GAGCCATCAG ACAAAAAGAC GTGCCAGCAA
    AACCCAGGTG TTCCTCAGCA ACAGACAAAG CCTTGTATAA AAGTGGAGCC TTCCAATCAC
    TACAATAACT TTAAGTACAA TGGAAATGGA GTTGTGGAGA GCTACTCAGT GTTGGGCAGC
    TGCAGGCCGT CCGACCCTTA CAGCATGAAC AGCGTTTACT CCTATCATTC TTTCTATGCA
    CAACCTAACC TGCCTTCCGT GAATGGCTTT CATTCAAAAT ATGCTCTTCC TCCCTTTGGA
    TTTTATGGCT TTCCTAACAA TCCTGTGGTT CCCAACCAGT TCATGAATTA TGGTACAAGT
    GATGCAAGGA ACAGTGGATG GATGAATAAT TGTTTTGAGA AAAAGCCAGA GCTTCAGTCA
    CTGGCAGATG GAATGAATCA AAGTTACGGG AGTGAGTTAT CAGAGCAGAG TTTTAGGAGA
    TCAAGTGAGG TACCACACCA CTACAGCCTC CAAAATCCCA GCAGTCAAAA ATCAGTTAAT
    GTTCCCCACC GAACTACTCC TGCACCAGTG GAGACTACTC CATATTCTAA TCTTCCCTGT
    TATAACAAAG TCATTAAAAA GGAGCCAGGT AGTGATCCAC TTGTAGATTC TTTCCAGAGA
    GCAAATAGTG TCCATTCACA TAGCCCAGGA GTTAATCACT CATTACAGGC CAGTGATCTG
    CCCATCTCAT ACAAAGCTAA TGGTGCTCTA TCATCATCAG GGAGGACTAA TGCAGAAAGT
    CCGTGCAGTA TGTTTATGCC CAATGATAAA AATGGTCTAG AAAAGAAAGA TTATTTTGGA
    GTCCATTCGA ATGCCCCTGG TCTGAAGGAC AAGCAGTGGC CTCCCTATGG AACTGATGTT
    TCAGTGAGAC AACATGATTC ACTTGACTCG CAGTCTCCAG GAAAAGTGTG GAGCTCATGC
    AAGCTGAGTG ACAGTTCCGC TGCCCTGCCC TCCTCTGCAA GTACACAGGA TAAGAACTGG
    AATGGTAGAC AGGTCAGTCT TAATCAGGGG ATGAAGGAGT CTGCGCTGTT CCAAGAAAAA
    CTCTGGAATT CAGTTGCTGC CAGTGACAGG TGCAGTGCCA CGCCCAGTGA CAGAAGTAGC
    ATCACCCCCT GTTCTGAGCT TCAGGACAAG AACTGGGGAT CATTTCCTAA TCCAACAGTA
    AACTCTTTGA AGACTGACTC CAGCCAGAAC CACTGGGATC CCTATAGCCT AGATGATAAT
    ATGGATGATG GCCAATCAAA GAGTGTAAAA GAGGAGGATG ATGAGGAGAT CTGGTCTGAT
    AGTGAGCACA ACTTTCTGGA TGAGAATATC GGAGGTGTGG CTGTAGCCCC AGGACATGGC
    TCTATCCTAA TTGAATGTGC AAGGAGAGAG CTCCATGCTA CCACTCCACT GAAAAAGCCC
    AACAGATGTC ATCCAACTCG AATCTCTCTT GTATTCTACC AACACAAGAA CTTAAACCAA
    CCTAACCATG GATTGGCACT TTGGGAGGCC AAAATGAAGC AGTTGGCAGA AAGGGCACGT
    GCAAGGGAGG AAGAGGCTGC CAAGCTAGGT ATCAAACAGG AAGTAAAAAG TTTGGGTAAA
    AAGAGGAAGT GGGGTGGTGC TGCCACTACA GAAACCCCAC CAGTTGAGAA GAAAGACTAT
    ACCCCTACCC GACAGGCAGC TACAATTTTA ACAGATTCTG CTACCACTTC ATTCTCCTAT
    GCCTATACCA AGGTTACGGG ACCATACAGT CGCTTTATAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53117
    Clone ID Related Accession (Same CDS sequence) XM_031897487.1 , XM_031897488.1 , XM_031897489.1 , XM_031897490.1
    Accession Version XM_031897487.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5325bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product methylcytosine dioxygenase tet3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGAAGCAG GGCCAGTTAA CCATGTTCAA GAAGGAAGGT TAAAGCAGGA ATGTGACTCT 
    ACCCTCCCAA GTAAGGGATG TGAGGATCTA GCAAACCAAT TATTAATGGA AGCAAATTCG
    TGGCTAAGCA ATACAGCTGC TCCTCAGGAT CCCTGCAACA AGCTCAACTG GGATAAACCC
    ACCATTCCCA ACCATGCTGC TAATAACAAT TCAAACCTAG AAGATGCCAA AAATCTAGTT
    GCATTCTCTG CTGTGGCTGA GGCTATGTCT ACCTATGGCA TGCCTGCATC AGGGACCCCC
    TCTTCGGTAT CACTGCAGCT ATATGAAAAG TTTAATTATG AGACAAACAG GGACAACTCT
    GGGCACTTGG AAGGCAATGC TCCATCATGT CCCGAAGACC TAAACACTCT AAAAGCAGCC
    CTTGCACTTG CAAAGCATGG CGTGAAACCA CCTAACTGTA ACTGTGACGG CCCTGAGTGC
    CCGGACTATC TAGAATGGCT GGAAAATAAA ATAAAATCTA CAGTTAAAGG TTCTCAGGAG
    AGCCCATTCC CTAATCTGGG TCAGGTCAGT AAAGAGCTGG TGCAAAAACA ATACCCTAAA
    GAACAGGTGC TAAATCTAGA AAATAAAAAT TCAACATGTC CTTCAGGTAA CCTTCCCTTT
    TCTCAAAATG CACTGAGTCT TGCTAAAGAA AAAAATATAA GTCTGCAAAC GGCCATTGCC
    ATTGAAGCTC TTACACAACT ATCATCTGCA CTTCCACAGA CAAACAATGA ATGCCCCAAT
    GCTCCTTCCC AACCTTTAAT AAACCCTCAT GATCAGTTAA CTCATTTCCC CTCTGCTAAA
    GGCAACCAGT TACCAATGTT ACCAGTGGCC CGCAATGAAC TGTTCCAGAA TCAGCAGTCC
    CAATTATACA CTGGCAAAAA TGCTCTTCCT GTGCCCCAAT CTCCAAGGCA AACTTCTTGG
    GAGCAGAACA AGAAATCTAG TTACCAAGAA GGACAATACA TTCCTGAAAA TTTATCACAT
    AGTTCTTCTG TGTTGCCCAG TGATGCTTCT ACCCCACAAA AACCTGAATT CCTTCAGCAG
    TGGGTCCAAA ATGCAGATCT TTTGAAGTCT CCATCTGATC CTATGACTGG GTTAAAGCAA
    TTGCTTGGGA ACACAGATGA GTACATCAAG TCTGTGTTTA AAGGGCCAGA GGCTTTGCCC
    AACAAAAAAA ATGTGAAGCC CAAACATACC ATTAAATCCA TAAAAAAAGA ATCCACCGAG
    TTTCTCAAAA TGTCACCAGA TCAACAGCTC TCTCAGCTTC TTCAAACAAA TGAATTTCAT
    CGTAATACCC AGGCTGCACT TCAGCAGCAT CTCCACCATA AACGCAATCT ATTTGTGGAC
    CCAAATGCTA TGGAAGCATG TACCCAAGAA CAGCAAAACT GGTGGGTGCC GAGTTCTCAA
    CAAGCTCCAG TTTCCAAGAC AACAGAGAAA CCTGTAAAAG AGAGGAAGAA AAGGCGACAG
    TCTCCTTCAC AAAAGCAAGT GGAACCAAAA CCGAAACCTC AACGTAAGCA AGTGCAAATA
    AAGAAACCCA AGGTGAAGGA AGGAAGTGCA GTCTTCATGC CTGTGAGCCA AATAAGCCTG
    GATACTTTTC GCAGAGTTGA AAAAGAAGAG AACCAGGGGA AAGAAATGGA TGCAGAGAAC
    TCTCTTCCTA ATAACGTACA AACAGAACTA CTTGAATCCC AGTCTTTACA ACTGACTGGA
    TCACAGGCAA ATCCTGATGA CAGAAAGACT GTAAACACTC AGGAAATGTG CAATGAAAAT
    CAGTCTAACA TTGGGAAGGC CAATAACTTT GCACTTTGTG TCAATCGGGC TAATTCTTTT
    GTTGCAAAAG ACCAATGTCC AACACCTTCC ACACATGATA CCAGCTCTAG CTCTGGACAA
    GGTGATTCTG CCAACCAGCA CCACACAAAT GTGAGTGATG TTCCTGGGCA AAATGACCTT
    AGCTGCCTTG ATGACAAGTT GGAAGATCTA ATAAGGCAGT TTGAAGCTGA GTTTGGGGAA
    GATTTCTCTC TACCTGGTTC TGCGGTCCCC TCTCAGAATG GTGAAGGACC GCCAAAACAA
    ACACCATCAG GAGATCCACA ATTTAAACTG CCCTTTCCTT CCCAGTTGTT ACCACCTGAG
    AATTCCACAA AGCCTGCCAC CCATTCCAAC CCTGCACTTT CAAATAATCC TGTATCACGT
    GAAGTTTCTA ATAACCTTGA TAGTCTGTTC TCATCAAAGT CTCCCAAGCA GATAAAAATC
    GAATCTTCTG GTGCTATAAC AGTGGTGTCT ACCACATGCT TCTACTCAGA GGAAAACCAA
    CATCTTGATG GAACTCCTAC AAAAAGTGAC CTGCCTTTCA ATCCTACTCT TAGTGGGTTT
    TTGGACTCGC CTCTCAAATA TCTGACATCT CCAACCAAGA GCTTAATAGA TACACCAGCT
    AAAAAGGCTC AAGCAGAATT TCCAACATGC GACTGTGTTG AACAAATTAA TGAAAAAGAT
    GAAGGCCCAT ATTATACACA TCTGGGTTCT GGACCAACAG TGGCTTCTAT CAGAGAGCTA
    ATGGAAGAGC GGTTTGGACA AAAAGGGGAT GCAATCCGCA TTGAAAAGGT GATCTACACA
    GGAAAAGAAG GAAAAAGCTC AAGGGGTTGT CCTATTGCAA AATGGGTCAT TCGCCGTCAG
    AGCGAGGATG AGAAGTTGAT GTGCCTTGTC CGGCAACGGG CAGGCCACCA TTGTGAAAAT
    GCAGTCATCA TTATCTTAAT TATGGCCTGG GAAGGAATTC CTCGCTCTCT AGGAGATTCT
    CTCTACAACG ACATCACTGA GACAATCACC AAGTATGGAA ATCCAACATC ACGGAGATGT
    GGACTGAATG ATGATCGAAC CTGTGCATGC CAAGGCAAGG ACCCAAATAC CTGTGGTGCT
    TCTTTCTCTT TTGGGTGCTC ATGGAGCATG TACTTTAATG GTTGCAAGTA TGCTCGTAGC
    AAGACCCCCC GGAAGTTCCG ACTTATAGGA GAAAATCCCA AGGAGGAAGA TGGCCTGAAG
    GATAACTTTC AGAATCTAGC TACAAAGGTT GCTCCAGTGT ACAAGATGCT TGCTCCTCAG
    GCTTATCAAA ATCAGGTTAA CAATGAGGAT ATTGCAATAG ATTGTCGGCT AGGTCTCAAG
    GAGGGGCGTC CTTTCTCTGG GGTGACTGCA TGCATGGATT TCTGTGCCCA TGCCCACAAA
    GACCAGCACA ATCTATACAA TGGATGCACT GTGGTCTGCA CTCTAACTAA GGAGGATAAC
    CGTATGATTG GCAGGGTTGC TGAGGATGAG CAGCTCCATG TACTTCCACT GTACAAGGTT
    TCCACAACTG ATGAATTTGG GAGTGAGGAG GGTCAACTTG AAAAGATAAA GAAAGGAGGA
    ATCCACGTGT TGAGCTCTTT TCCCCGGGAG GTACGCAAGC TTTCAGAACC AGCAAAGTCT
    TGCAGGCAGA GACAACTAGA AGCCAAGAAG GCCGCAGCCG AAAAGAAGAA ACTTCAAAAG
    GAGAAACTGG TCTCTCCGGA TAAGACCAAG CAGGAGCCAT CAGACAAAAA GACGTGCCAG
    CAAAACCCAG GTGTTCCTCA GCAACAGACA AAGCCTTGTA TAAAAGTGGA GCCTTCCAAT
    CACTACAATA ACTTTAAGTA CAATGGAAAT GGAGTTGTGG AGAGCTACTC AGTGTTGGGC
    AGCTGCAGGC CGTCCGACCC TTACAGCATG AACAGCGTTT ACTCCTATCA TTCTTTCTAT
    GCACAACCTA ACCTGCCTTC CGTGAATGGC TTTCATTCAA AATATGCTCT TCCTCCCTTT
    GGATTTTATG GCTTTCCTAA CAATCCTGTG GTTCCCAACC AGTTCATGAA TTATGGTACA
    AGTGATGCAA GGAACAGTGG ATGGATGAAT AATTGTTTTG AGAAAAAGCC AGAGCTTCAG
    TCACTGGCAG ATGGAATGAA TCAAAGTTAC GGGAGTGAGT TATCAGAGCA GAGTTTTAGG
    AGATCAAGTG AGGTACCACA CCACTACAGC CTCCAAAATC CCAGCAGTCA AAAATCAGTT
    AATGTTCCCC ACCGAACTAC TCCTGCACCA GTGGAGACTA CTCCATATTC TAATCTTCCC
    TGTTATAACA AAGTCATTAA AAAGGAGCCA GGTAGTGATC CACTTGTAGA TTCTTTCCAG
    AGAGCAAATA GTGTCCATTC ACATAGCCCA GGAGTTAATC ACTCATTACA GGCCAGTGAT
    CTGCCCATCT CATACAAAGC TAATGGTGCT CTATCATCAT CAGGGAGGAC TAATGCAGAA
    AGTCCGTGCA GTATGTTTAT GCCCAATGAT AAAAATGGTC TAGAAAAGAA AGATTATTTT
    GGAGTCCATT CGAATGCCCC TGGTCTGAAG GACAAGCAGT GGCCTCCCTA TGGAACTGAT
    GTTTCAGTGA GACAACATGA TTCACTTGAC TCGCAGTCTC CAGGAAAAGT GTGGAGCTCA
    TGCAAGCTGA GTGACAGTTC CGCTGCCCTG CCCTCCTCTG CAAGTACACA GGATAAGAAC
    TGGAATGGTA GACAGGTCAG TCTTAATCAG GGGATGAAGG AGTCTGCGCT GTTCCAAGAA
    AAACTCTGGA ATTCAGTTGC TGCCAGTGAC AGGTGCAGTG CCACGCCCAG TGACAGAAGT
    AGCATCACCC CCTGTTCTGA GCTTCAGGAC AAGAACTGGG GATCATTTCC TAATCCAACA
    GTAAACTCTT TGAAGACTGA CTCCAGCCAG AACCACTGGG ATCCCTATAG CCTAGATGAT
    AATATGGATG ATGGCCAATC AAAGAGTGTA AAAGAGGAGG ATGATGAGGA GATCTGGTCT
    GATAGTGAGC ACAACTTTCT GGATGAGAAT ATCGGAGGTG TGGCTGTAGC CCCAGGACAT
    GGCTCTATCC TAATTGAATG TGCAAGGAGA GAGCTCCATG CTACCACTCC ACTGAAAAAG
    CCCAACAGAT GTCATCCAAC TCGAATCTCT CTTGTATTCT ACCAACACAA GAACTTAAAC
    CAACCTAACC ATGGATTGGC ACTTTGGGAG GCCAAAATGA AGCAGTTGGC AGAAAGGGCA
    CGTGCAAGGG AGGAAGAGGC TGCCAAGCTA GGTATCAAAC AGGAAGTAAA AAGTTTGGGT
    AAAAAGAGGA AGTGGGGTGG TGCTGCCACT ACAGAAACCC CACCAGTTGA GAAGAAAGAC
    TATACCCCTA CCCGACAGGC AGCTACAATT TTAACAGATT CTGCTACCAC TTCATTCTCC
    TATGCCTATA CCAAGGTTAC GGGACCATAC AGTCGCTTTA TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753347.1
    CDS559..5883
    Translation

    Target ORF information:

    RefSeq Version XM_031897487.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis tet methylcytosine dioxygenase 3 (tet3), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897487.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGAAGCAG GGCCAGTTAA CCATGTTCAA GAAGGAAGGT TAAAGCAGGA ATGTGACTCT 
    ACCCTCCCAA GTAAGGGATG TGAGGATCTA GCAAACCAAT TATTAATGGA AGCAAATTCG
    TGGCTAAGCA ATACAGCTGC TCCTCAGGAT CCCTGCAACA AGCTCAACTG GGATAAACCC
    ACCATTCCCA ACCATGCTGC TAATAACAAT TCAAACCTAG AAGATGCCAA AAATCTAGTT
    GCATTCTCTG CTGTGGCTGA GGCTATGTCT ACCTATGGCA TGCCTGCATC AGGGACCCCC
    TCTTCGGTAT CACTGCAGCT ATATGAAAAG TTTAATTATG AGACAAACAG GGACAACTCT
    GGGCACTTGG AAGGCAATGC TCCATCATGT CCCGAAGACC TAAACACTCT AAAAGCAGCC
    CTTGCACTTG CAAAGCATGG CGTGAAACCA CCTAACTGTA ACTGTGACGG CCCTGAGTGC
    CCGGACTATC TAGAATGGCT GGAAAATAAA ATAAAATCTA CAGTTAAAGG TTCTCAGGAG
    AGCCCATTCC CTAATCTGGG TCAGGTCAGT AAAGAGCTGG TGCAAAAACA ATACCCTAAA
    GAACAGGTGC TAAATCTAGA AAATAAAAAT TCAACATGTC CTTCAGGTAA CCTTCCCTTT
    TCTCAAAATG CACTGAGTCT TGCTAAAGAA AAAAATATAA GTCTGCAAAC GGCCATTGCC
    ATTGAAGCTC TTACACAACT ATCATCTGCA CTTCCACAGA CAAACAATGA ATGCCCCAAT
    GCTCCTTCCC AACCTTTAAT AAACCCTCAT GATCAGTTAA CTCATTTCCC CTCTGCTAAA
    GGCAACCAGT TACCAATGTT ACCAGTGGCC CGCAATGAAC TGTTCCAGAA TCAGCAGTCC
    CAATTATACA CTGGCAAAAA TGCTCTTCCT GTGCCCCAAT CTCCAAGGCA AACTTCTTGG
    GAGCAGAACA AGAAATCTAG TTACCAAGAA GGACAATACA TTCCTGAAAA TTTATCACAT
    AGTTCTTCTG TGTTGCCCAG TGATGCTTCT ACCCCACAAA AACCTGAATT CCTTCAGCAG
    TGGGTCCAAA ATGCAGATCT TTTGAAGTCT CCATCTGATC CTATGACTGG GTTAAAGCAA
    TTGCTTGGGA ACACAGATGA GTACATCAAG TCTGTGTTTA AAGGGCCAGA GGCTTTGCCC
    AACAAAAAAA ATGTGAAGCC CAAACATACC ATTAAATCCA TAAAAAAAGA ATCCACCGAG
    TTTCTCAAAA TGTCACCAGA TCAACAGCTC TCTCAGCTTC TTCAAACAAA TGAATTTCAT
    CGTAATACCC AGGCTGCACT TCAGCAGCAT CTCCACCATA AACGCAATCT ATTTGTGGAC
    CCAAATGCTA TGGAAGCATG TACCCAAGAA CAGCAAAACT GGTGGGTGCC GAGTTCTCAA
    CAAGCTCCAG TTTCCAAGAC AACAGAGAAA CCTGTAAAAG AGAGGAAGAA AAGGCGACAG
    TCTCCTTCAC AAAAGCAAGT GGAACCAAAA CCGAAACCTC AACGTAAGCA AGTGCAAATA
    AAGAAACCCA AGGTGAAGGA AGGAAGTGCA GTCTTCATGC CTGTGAGCCA AATAAGCCTG
    GATACTTTTC GCAGAGTTGA AAAAGAAGAG AACCAGGGGA AAGAAATGGA TGCAGAGAAC
    TCTCTTCCTA ATAACGTACA AACAGAACTA CTTGAATCCC AGTCTTTACA ACTGACTGGA
    TCACAGGCAA ATCCTGATGA CAGAAAGACT GTAAACACTC AGGAAATGTG CAATGAAAAT
    CAGTCTAACA TTGGGAAGGC CAATAACTTT GCACTTTGTG TCAATCGGGC TAATTCTTTT
    GTTGCAAAAG ACCAATGTCC AACACCTTCC ACACATGATA CCAGCTCTAG CTCTGGACAA
    GGTGATTCTG CCAACCAGCA CCACACAAAT GTGAGTGATG TTCCTGGGCA AAATGACCTT
    AGCTGCCTTG ATGACAAGTT GGAAGATCTA ATAAGGCAGT TTGAAGCTGA GTTTGGGGAA
    GATTTCTCTC TACCTGGTTC TGCGGTCCCC TCTCAGAATG GTGAAGGACC GCCAAAACAA
    ACACCATCAG GAGATCCACA ATTTAAACTG CCCTTTCCTT CCCAGTTGTT ACCACCTGAG
    AATTCCACAA AGCCTGCCAC CCATTCCAAC CCTGCACTTT CAAATAATCC TGTATCACGT
    GAAGTTTCTA ATAACCTTGA TAGTCTGTTC TCATCAAAGT CTCCCAAGCA GATAAAAATC
    GAATCTTCTG GTGCTATAAC AGTGGTGTCT ACCACATGCT TCTACTCAGA GGAAAACCAA
    CATCTTGATG GAACTCCTAC AAAAAGTGAC CTGCCTTTCA ATCCTACTCT TAGTGGGTTT
    TTGGACTCGC CTCTCAAATA TCTGACATCT CCAACCAAGA GCTTAATAGA TACACCAGCT
    AAAAAGGCTC AAGCAGAATT TCCAACATGC GACTGTGTTG AACAAATTAA TGAAAAAGAT
    GAAGGCCCAT ATTATACACA TCTGGGTTCT GGACCAACAG TGGCTTCTAT CAGAGAGCTA
    ATGGAAGAGC GGTTTGGACA AAAAGGGGAT GCAATCCGCA TTGAAAAGGT GATCTACACA
    GGAAAAGAAG GAAAAAGCTC AAGGGGTTGT CCTATTGCAA AATGGGTCAT TCGCCGTCAG
    AGCGAGGATG AGAAGTTGAT GTGCCTTGTC CGGCAACGGG CAGGCCACCA TTGTGAAAAT
    GCAGTCATCA TTATCTTAAT TATGGCCTGG GAAGGAATTC CTCGCTCTCT AGGAGATTCT
    CTCTACAACG ACATCACTGA GACAATCACC AAGTATGGAA ATCCAACATC ACGGAGATGT
    GGACTGAATG ATGATCGAAC CTGTGCATGC CAAGGCAAGG ACCCAAATAC CTGTGGTGCT
    TCTTTCTCTT TTGGGTGCTC ATGGAGCATG TACTTTAATG GTTGCAAGTA TGCTCGTAGC
    AAGACCCCCC GGAAGTTCCG ACTTATAGGA GAAAATCCCA AGGAGGAAGA TGGCCTGAAG
    GATAACTTTC AGAATCTAGC TACAAAGGTT GCTCCAGTGT ACAAGATGCT TGCTCCTCAG
    GCTTATCAAA ATCAGGTTAA CAATGAGGAT ATTGCAATAG ATTGTCGGCT AGGTCTCAAG
    GAGGGGCGTC CTTTCTCTGG GGTGACTGCA TGCATGGATT TCTGTGCCCA TGCCCACAAA
    GACCAGCACA ATCTATACAA TGGATGCACT GTGGTCTGCA CTCTAACTAA GGAGGATAAC
    CGTATGATTG GCAGGGTTGC TGAGGATGAG CAGCTCCATG TACTTCCACT GTACAAGGTT
    TCCACAACTG ATGAATTTGG GAGTGAGGAG GGTCAACTTG AAAAGATAAA GAAAGGAGGA
    ATCCACGTGT TGAGCTCTTT TCCCCGGGAG GTACGCAAGC TTTCAGAACC AGCAAAGTCT
    TGCAGGCAGA GACAACTAGA AGCCAAGAAG GCCGCAGCCG AAAAGAAGAA ACTTCAAAAG
    GAGAAACTGG TCTCTCCGGA TAAGACCAAG CAGGAGCCAT CAGACAAAAA GACGTGCCAG
    CAAAACCCAG GTGTTCCTCA GCAACAGACA AAGCCTTGTA TAAAAGTGGA GCCTTCCAAT
    CACTACAATA ACTTTAAGTA CAATGGAAAT GGAGTTGTGG AGAGCTACTC AGTGTTGGGC
    AGCTGCAGGC CGTCCGACCC TTACAGCATG AACAGCGTTT ACTCCTATCA TTCTTTCTAT
    GCACAACCTA ACCTGCCTTC CGTGAATGGC TTTCATTCAA AATATGCTCT TCCTCCCTTT
    GGATTTTATG GCTTTCCTAA CAATCCTGTG GTTCCCAACC AGTTCATGAA TTATGGTACA
    AGTGATGCAA GGAACAGTGG ATGGATGAAT AATTGTTTTG AGAAAAAGCC AGAGCTTCAG
    TCACTGGCAG ATGGAATGAA TCAAAGTTAC GGGAGTGAGT TATCAGAGCA GAGTTTTAGG
    AGATCAAGTG AGGTACCACA CCACTACAGC CTCCAAAATC CCAGCAGTCA AAAATCAGTT
    AATGTTCCCC ACCGAACTAC TCCTGCACCA GTGGAGACTA CTCCATATTC TAATCTTCCC
    TGTTATAACA AAGTCATTAA AAAGGAGCCA GGTAGTGATC CACTTGTAGA TTCTTTCCAG
    AGAGCAAATA GTGTCCATTC ACATAGCCCA GGAGTTAATC ACTCATTACA GGCCAGTGAT
    CTGCCCATCT CATACAAAGC TAATGGTGCT CTATCATCAT CAGGGAGGAC TAATGCAGAA
    AGTCCGTGCA GTATGTTTAT GCCCAATGAT AAAAATGGTC TAGAAAAGAA AGATTATTTT
    GGAGTCCATT CGAATGCCCC TGGTCTGAAG GACAAGCAGT GGCCTCCCTA TGGAACTGAT
    GTTTCAGTGA GACAACATGA TTCACTTGAC TCGCAGTCTC CAGGAAAAGT GTGGAGCTCA
    TGCAAGCTGA GTGACAGTTC CGCTGCCCTG CCCTCCTCTG CAAGTACACA GGATAAGAAC
    TGGAATGGTA GACAGGTCAG TCTTAATCAG GGGATGAAGG AGTCTGCGCT GTTCCAAGAA
    AAACTCTGGA ATTCAGTTGC TGCCAGTGAC AGGTGCAGTG CCACGCCCAG TGACAGAAGT
    AGCATCACCC CCTGTTCTGA GCTTCAGGAC AAGAACTGGG GATCATTTCC TAATCCAACA
    GTAAACTCTT TGAAGACTGA CTCCAGCCAG AACCACTGGG ATCCCTATAG CCTAGATGAT
    AATATGGATG ATGGCCAATC AAAGAGTGTA AAAGAGGAGG ATGATGAGGA GATCTGGTCT
    GATAGTGAGC ACAACTTTCT GGATGAGAAT ATCGGAGGTG TGGCTGTAGC CCCAGGACAT
    GGCTCTATCC TAATTGAATG TGCAAGGAGA GAGCTCCATG CTACCACTCC ACTGAAAAAG
    CCCAACAGAT GTCATCCAAC TCGAATCTCT CTTGTATTCT ACCAACACAA GAACTTAAAC
    CAACCTAACC ATGGATTGGC ACTTTGGGAG GCCAAAATGA AGCAGTTGGC AGAAAGGGCA
    CGTGCAAGGG AGGAAGAGGC TGCCAAGCTA GGTATCAAAC AGGAAGTAAA AAGTTTGGGT
    AAAAAGAGGA AGTGGGGTGG TGCTGCCACT ACAGAAACCC CACCAGTTGA GAAGAAAGAC
    TATACCCCTA CCCGACAGGC AGCTACAATT TTAACAGATT CTGCTACCAC TTCATTCTCC
    TATGCCTATA CCAAGGTTAC GGGACCATAC AGTCGCTTTA TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53117
    Clone ID Related Accession (Same CDS sequence) XM_031897487.1 , XM_031897488.1 , XM_031897489.1 , XM_031897490.1
    Accession Version XM_031897488.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5325bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product methylcytosine dioxygenase tet3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGAAGCAG GGCCAGTTAA CCATGTTCAA GAAGGAAGGT TAAAGCAGGA ATGTGACTCT 
    ACCCTCCCAA GTAAGGGATG TGAGGATCTA GCAAACCAAT TATTAATGGA AGCAAATTCG
    TGGCTAAGCA ATACAGCTGC TCCTCAGGAT CCCTGCAACA AGCTCAACTG GGATAAACCC
    ACCATTCCCA ACCATGCTGC TAATAACAAT TCAAACCTAG AAGATGCCAA AAATCTAGTT
    GCATTCTCTG CTGTGGCTGA GGCTATGTCT ACCTATGGCA TGCCTGCATC AGGGACCCCC
    TCTTCGGTAT CACTGCAGCT ATATGAAAAG TTTAATTATG AGACAAACAG GGACAACTCT
    GGGCACTTGG AAGGCAATGC TCCATCATGT CCCGAAGACC TAAACACTCT AAAAGCAGCC
    CTTGCACTTG CAAAGCATGG CGTGAAACCA CCTAACTGTA ACTGTGACGG CCCTGAGTGC
    CCGGACTATC TAGAATGGCT GGAAAATAAA ATAAAATCTA CAGTTAAAGG TTCTCAGGAG
    AGCCCATTCC CTAATCTGGG TCAGGTCAGT AAAGAGCTGG TGCAAAAACA ATACCCTAAA
    GAACAGGTGC TAAATCTAGA AAATAAAAAT TCAACATGTC CTTCAGGTAA CCTTCCCTTT
    TCTCAAAATG CACTGAGTCT TGCTAAAGAA AAAAATATAA GTCTGCAAAC GGCCATTGCC
    ATTGAAGCTC TTACACAACT ATCATCTGCA CTTCCACAGA CAAACAATGA ATGCCCCAAT
    GCTCCTTCCC AACCTTTAAT AAACCCTCAT GATCAGTTAA CTCATTTCCC CTCTGCTAAA
    GGCAACCAGT TACCAATGTT ACCAGTGGCC CGCAATGAAC TGTTCCAGAA TCAGCAGTCC
    CAATTATACA CTGGCAAAAA TGCTCTTCCT GTGCCCCAAT CTCCAAGGCA AACTTCTTGG
    GAGCAGAACA AGAAATCTAG TTACCAAGAA GGACAATACA TTCCTGAAAA TTTATCACAT
    AGTTCTTCTG TGTTGCCCAG TGATGCTTCT ACCCCACAAA AACCTGAATT CCTTCAGCAG
    TGGGTCCAAA ATGCAGATCT TTTGAAGTCT CCATCTGATC CTATGACTGG GTTAAAGCAA
    TTGCTTGGGA ACACAGATGA GTACATCAAG TCTGTGTTTA AAGGGCCAGA GGCTTTGCCC
    AACAAAAAAA ATGTGAAGCC CAAACATACC ATTAAATCCA TAAAAAAAGA ATCCACCGAG
    TTTCTCAAAA TGTCACCAGA TCAACAGCTC TCTCAGCTTC TTCAAACAAA TGAATTTCAT
    CGTAATACCC AGGCTGCACT TCAGCAGCAT CTCCACCATA AACGCAATCT ATTTGTGGAC
    CCAAATGCTA TGGAAGCATG TACCCAAGAA CAGCAAAACT GGTGGGTGCC GAGTTCTCAA
    CAAGCTCCAG TTTCCAAGAC AACAGAGAAA CCTGTAAAAG AGAGGAAGAA AAGGCGACAG
    TCTCCTTCAC AAAAGCAAGT GGAACCAAAA CCGAAACCTC AACGTAAGCA AGTGCAAATA
    AAGAAACCCA AGGTGAAGGA AGGAAGTGCA GTCTTCATGC CTGTGAGCCA AATAAGCCTG
    GATACTTTTC GCAGAGTTGA AAAAGAAGAG AACCAGGGGA AAGAAATGGA TGCAGAGAAC
    TCTCTTCCTA ATAACGTACA AACAGAACTA CTTGAATCCC AGTCTTTACA ACTGACTGGA
    TCACAGGCAA ATCCTGATGA CAGAAAGACT GTAAACACTC AGGAAATGTG CAATGAAAAT
    CAGTCTAACA TTGGGAAGGC CAATAACTTT GCACTTTGTG TCAATCGGGC TAATTCTTTT
    GTTGCAAAAG ACCAATGTCC AACACCTTCC ACACATGATA CCAGCTCTAG CTCTGGACAA
    GGTGATTCTG CCAACCAGCA CCACACAAAT GTGAGTGATG TTCCTGGGCA AAATGACCTT
    AGCTGCCTTG ATGACAAGTT GGAAGATCTA ATAAGGCAGT TTGAAGCTGA GTTTGGGGAA
    GATTTCTCTC TACCTGGTTC TGCGGTCCCC TCTCAGAATG GTGAAGGACC GCCAAAACAA
    ACACCATCAG GAGATCCACA ATTTAAACTG CCCTTTCCTT CCCAGTTGTT ACCACCTGAG
    AATTCCACAA AGCCTGCCAC CCATTCCAAC CCTGCACTTT CAAATAATCC TGTATCACGT
    GAAGTTTCTA ATAACCTTGA TAGTCTGTTC TCATCAAAGT CTCCCAAGCA GATAAAAATC
    GAATCTTCTG GTGCTATAAC AGTGGTGTCT ACCACATGCT TCTACTCAGA GGAAAACCAA
    CATCTTGATG GAACTCCTAC AAAAAGTGAC CTGCCTTTCA ATCCTACTCT TAGTGGGTTT
    TTGGACTCGC CTCTCAAATA TCTGACATCT CCAACCAAGA GCTTAATAGA TACACCAGCT
    AAAAAGGCTC AAGCAGAATT TCCAACATGC GACTGTGTTG AACAAATTAA TGAAAAAGAT
    GAAGGCCCAT ATTATACACA TCTGGGTTCT GGACCAACAG TGGCTTCTAT CAGAGAGCTA
    ATGGAAGAGC GGTTTGGACA AAAAGGGGAT GCAATCCGCA TTGAAAAGGT GATCTACACA
    GGAAAAGAAG GAAAAAGCTC AAGGGGTTGT CCTATTGCAA AATGGGTCAT TCGCCGTCAG
    AGCGAGGATG AGAAGTTGAT GTGCCTTGTC CGGCAACGGG CAGGCCACCA TTGTGAAAAT
    GCAGTCATCA TTATCTTAAT TATGGCCTGG GAAGGAATTC CTCGCTCTCT AGGAGATTCT
    CTCTACAACG ACATCACTGA GACAATCACC AAGTATGGAA ATCCAACATC ACGGAGATGT
    GGACTGAATG ATGATCGAAC CTGTGCATGC CAAGGCAAGG ACCCAAATAC CTGTGGTGCT
    TCTTTCTCTT TTGGGTGCTC ATGGAGCATG TACTTTAATG GTTGCAAGTA TGCTCGTAGC
    AAGACCCCCC GGAAGTTCCG ACTTATAGGA GAAAATCCCA AGGAGGAAGA TGGCCTGAAG
    GATAACTTTC AGAATCTAGC TACAAAGGTT GCTCCAGTGT ACAAGATGCT TGCTCCTCAG
    GCTTATCAAA ATCAGGTTAA CAATGAGGAT ATTGCAATAG ATTGTCGGCT AGGTCTCAAG
    GAGGGGCGTC CTTTCTCTGG GGTGACTGCA TGCATGGATT TCTGTGCCCA TGCCCACAAA
    GACCAGCACA ATCTATACAA TGGATGCACT GTGGTCTGCA CTCTAACTAA GGAGGATAAC
    CGTATGATTG GCAGGGTTGC TGAGGATGAG CAGCTCCATG TACTTCCACT GTACAAGGTT
    TCCACAACTG ATGAATTTGG GAGTGAGGAG GGTCAACTTG AAAAGATAAA GAAAGGAGGA
    ATCCACGTGT TGAGCTCTTT TCCCCGGGAG GTACGCAAGC TTTCAGAACC AGCAAAGTCT
    TGCAGGCAGA GACAACTAGA AGCCAAGAAG GCCGCAGCCG AAAAGAAGAA ACTTCAAAAG
    GAGAAACTGG TCTCTCCGGA TAAGACCAAG CAGGAGCCAT CAGACAAAAA GACGTGCCAG
    CAAAACCCAG GTGTTCCTCA GCAACAGACA AAGCCTTGTA TAAAAGTGGA GCCTTCCAAT
    CACTACAATA ACTTTAAGTA CAATGGAAAT GGAGTTGTGG AGAGCTACTC AGTGTTGGGC
    AGCTGCAGGC CGTCCGACCC TTACAGCATG AACAGCGTTT ACTCCTATCA TTCTTTCTAT
    GCACAACCTA ACCTGCCTTC CGTGAATGGC TTTCATTCAA AATATGCTCT TCCTCCCTTT
    GGATTTTATG GCTTTCCTAA CAATCCTGTG GTTCCCAACC AGTTCATGAA TTATGGTACA
    AGTGATGCAA GGAACAGTGG ATGGATGAAT AATTGTTTTG AGAAAAAGCC AGAGCTTCAG
    TCACTGGCAG ATGGAATGAA TCAAAGTTAC GGGAGTGAGT TATCAGAGCA GAGTTTTAGG
    AGATCAAGTG AGGTACCACA CCACTACAGC CTCCAAAATC CCAGCAGTCA AAAATCAGTT
    AATGTTCCCC ACCGAACTAC TCCTGCACCA GTGGAGACTA CTCCATATTC TAATCTTCCC
    TGTTATAACA AAGTCATTAA AAAGGAGCCA GGTAGTGATC CACTTGTAGA TTCTTTCCAG
    AGAGCAAATA GTGTCCATTC ACATAGCCCA GGAGTTAATC ACTCATTACA GGCCAGTGAT
    CTGCCCATCT CATACAAAGC TAATGGTGCT CTATCATCAT CAGGGAGGAC TAATGCAGAA
    AGTCCGTGCA GTATGTTTAT GCCCAATGAT AAAAATGGTC TAGAAAAGAA AGATTATTTT
    GGAGTCCATT CGAATGCCCC TGGTCTGAAG GACAAGCAGT GGCCTCCCTA TGGAACTGAT
    GTTTCAGTGA GACAACATGA TTCACTTGAC TCGCAGTCTC CAGGAAAAGT GTGGAGCTCA
    TGCAAGCTGA GTGACAGTTC CGCTGCCCTG CCCTCCTCTG CAAGTACACA GGATAAGAAC
    TGGAATGGTA GACAGGTCAG TCTTAATCAG GGGATGAAGG AGTCTGCGCT GTTCCAAGAA
    AAACTCTGGA ATTCAGTTGC TGCCAGTGAC AGGTGCAGTG CCACGCCCAG TGACAGAAGT
    AGCATCACCC CCTGTTCTGA GCTTCAGGAC AAGAACTGGG GATCATTTCC TAATCCAACA
    GTAAACTCTT TGAAGACTGA CTCCAGCCAG AACCACTGGG ATCCCTATAG CCTAGATGAT
    AATATGGATG ATGGCCAATC AAAGAGTGTA AAAGAGGAGG ATGATGAGGA GATCTGGTCT
    GATAGTGAGC ACAACTTTCT GGATGAGAAT ATCGGAGGTG TGGCTGTAGC CCCAGGACAT
    GGCTCTATCC TAATTGAATG TGCAAGGAGA GAGCTCCATG CTACCACTCC ACTGAAAAAG
    CCCAACAGAT GTCATCCAAC TCGAATCTCT CTTGTATTCT ACCAACACAA GAACTTAAAC
    CAACCTAACC ATGGATTGGC ACTTTGGGAG GCCAAAATGA AGCAGTTGGC AGAAAGGGCA
    CGTGCAAGGG AGGAAGAGGC TGCCAAGCTA GGTATCAAAC AGGAAGTAAA AAGTTTGGGT
    AAAAAGAGGA AGTGGGGTGG TGCTGCCACT ACAGAAACCC CACCAGTTGA GAAGAAAGAC
    TATACCCCTA CCCGACAGGC AGCTACAATT TTAACAGATT CTGCTACCAC TTCATTCTCC
    TATGCCTATA CCAAGGTTAC GGGACCATAC AGTCGCTTTA TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753348.1
    CDS2048..7372
    Translation

    Target ORF information:

    RefSeq Version XM_031897488.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis tet methylcytosine dioxygenase 3 (tet3), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897488.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGAAGCAG GGCCAGTTAA CCATGTTCAA GAAGGAAGGT TAAAGCAGGA ATGTGACTCT 
    ACCCTCCCAA GTAAGGGATG TGAGGATCTA GCAAACCAAT TATTAATGGA AGCAAATTCG
    TGGCTAAGCA ATACAGCTGC TCCTCAGGAT CCCTGCAACA AGCTCAACTG GGATAAACCC
    ACCATTCCCA ACCATGCTGC TAATAACAAT TCAAACCTAG AAGATGCCAA AAATCTAGTT
    GCATTCTCTG CTGTGGCTGA GGCTATGTCT ACCTATGGCA TGCCTGCATC AGGGACCCCC
    TCTTCGGTAT CACTGCAGCT ATATGAAAAG TTTAATTATG AGACAAACAG GGACAACTCT
    GGGCACTTGG AAGGCAATGC TCCATCATGT CCCGAAGACC TAAACACTCT AAAAGCAGCC
    CTTGCACTTG CAAAGCATGG CGTGAAACCA CCTAACTGTA ACTGTGACGG CCCTGAGTGC
    CCGGACTATC TAGAATGGCT GGAAAATAAA ATAAAATCTA CAGTTAAAGG TTCTCAGGAG
    AGCCCATTCC CTAATCTGGG TCAGGTCAGT AAAGAGCTGG TGCAAAAACA ATACCCTAAA
    GAACAGGTGC TAAATCTAGA AAATAAAAAT TCAACATGTC CTTCAGGTAA CCTTCCCTTT
    TCTCAAAATG CACTGAGTCT TGCTAAAGAA AAAAATATAA GTCTGCAAAC GGCCATTGCC
    ATTGAAGCTC TTACACAACT ATCATCTGCA CTTCCACAGA CAAACAATGA ATGCCCCAAT
    GCTCCTTCCC AACCTTTAAT AAACCCTCAT GATCAGTTAA CTCATTTCCC CTCTGCTAAA
    GGCAACCAGT TACCAATGTT ACCAGTGGCC CGCAATGAAC TGTTCCAGAA TCAGCAGTCC
    CAATTATACA CTGGCAAAAA TGCTCTTCCT GTGCCCCAAT CTCCAAGGCA AACTTCTTGG
    GAGCAGAACA AGAAATCTAG TTACCAAGAA GGACAATACA TTCCTGAAAA TTTATCACAT
    AGTTCTTCTG TGTTGCCCAG TGATGCTTCT ACCCCACAAA AACCTGAATT CCTTCAGCAG
    TGGGTCCAAA ATGCAGATCT TTTGAAGTCT CCATCTGATC CTATGACTGG GTTAAAGCAA
    TTGCTTGGGA ACACAGATGA GTACATCAAG TCTGTGTTTA AAGGGCCAGA GGCTTTGCCC
    AACAAAAAAA ATGTGAAGCC CAAACATACC ATTAAATCCA TAAAAAAAGA ATCCACCGAG
    TTTCTCAAAA TGTCACCAGA TCAACAGCTC TCTCAGCTTC TTCAAACAAA TGAATTTCAT
    CGTAATACCC AGGCTGCACT TCAGCAGCAT CTCCACCATA AACGCAATCT ATTTGTGGAC
    CCAAATGCTA TGGAAGCATG TACCCAAGAA CAGCAAAACT GGTGGGTGCC GAGTTCTCAA
    CAAGCTCCAG TTTCCAAGAC AACAGAGAAA CCTGTAAAAG AGAGGAAGAA AAGGCGACAG
    TCTCCTTCAC AAAAGCAAGT GGAACCAAAA CCGAAACCTC AACGTAAGCA AGTGCAAATA
    AAGAAACCCA AGGTGAAGGA AGGAAGTGCA GTCTTCATGC CTGTGAGCCA AATAAGCCTG
    GATACTTTTC GCAGAGTTGA AAAAGAAGAG AACCAGGGGA AAGAAATGGA TGCAGAGAAC
    TCTCTTCCTA ATAACGTACA AACAGAACTA CTTGAATCCC AGTCTTTACA ACTGACTGGA
    TCACAGGCAA ATCCTGATGA CAGAAAGACT GTAAACACTC AGGAAATGTG CAATGAAAAT
    CAGTCTAACA TTGGGAAGGC CAATAACTTT GCACTTTGTG TCAATCGGGC TAATTCTTTT
    GTTGCAAAAG ACCAATGTCC AACACCTTCC ACACATGATA CCAGCTCTAG CTCTGGACAA
    GGTGATTCTG CCAACCAGCA CCACACAAAT GTGAGTGATG TTCCTGGGCA AAATGACCTT
    AGCTGCCTTG ATGACAAGTT GGAAGATCTA ATAAGGCAGT TTGAAGCTGA GTTTGGGGAA
    GATTTCTCTC TACCTGGTTC TGCGGTCCCC TCTCAGAATG GTGAAGGACC GCCAAAACAA
    ACACCATCAG GAGATCCACA ATTTAAACTG CCCTTTCCTT CCCAGTTGTT ACCACCTGAG
    AATTCCACAA AGCCTGCCAC CCATTCCAAC CCTGCACTTT CAAATAATCC TGTATCACGT
    GAAGTTTCTA ATAACCTTGA TAGTCTGTTC TCATCAAAGT CTCCCAAGCA GATAAAAATC
    GAATCTTCTG GTGCTATAAC AGTGGTGTCT ACCACATGCT TCTACTCAGA GGAAAACCAA
    CATCTTGATG GAACTCCTAC AAAAAGTGAC CTGCCTTTCA ATCCTACTCT TAGTGGGTTT
    TTGGACTCGC CTCTCAAATA TCTGACATCT CCAACCAAGA GCTTAATAGA TACACCAGCT
    AAAAAGGCTC AAGCAGAATT TCCAACATGC GACTGTGTTG AACAAATTAA TGAAAAAGAT
    GAAGGCCCAT ATTATACACA TCTGGGTTCT GGACCAACAG TGGCTTCTAT CAGAGAGCTA
    ATGGAAGAGC GGTTTGGACA AAAAGGGGAT GCAATCCGCA TTGAAAAGGT GATCTACACA
    GGAAAAGAAG GAAAAAGCTC AAGGGGTTGT CCTATTGCAA AATGGGTCAT TCGCCGTCAG
    AGCGAGGATG AGAAGTTGAT GTGCCTTGTC CGGCAACGGG CAGGCCACCA TTGTGAAAAT
    GCAGTCATCA TTATCTTAAT TATGGCCTGG GAAGGAATTC CTCGCTCTCT AGGAGATTCT
    CTCTACAACG ACATCACTGA GACAATCACC AAGTATGGAA ATCCAACATC ACGGAGATGT
    GGACTGAATG ATGATCGAAC CTGTGCATGC CAAGGCAAGG ACCCAAATAC CTGTGGTGCT
    TCTTTCTCTT TTGGGTGCTC ATGGAGCATG TACTTTAATG GTTGCAAGTA TGCTCGTAGC
    AAGACCCCCC GGAAGTTCCG ACTTATAGGA GAAAATCCCA AGGAGGAAGA TGGCCTGAAG
    GATAACTTTC AGAATCTAGC TACAAAGGTT GCTCCAGTGT ACAAGATGCT TGCTCCTCAG
    GCTTATCAAA ATCAGGTTAA CAATGAGGAT ATTGCAATAG ATTGTCGGCT AGGTCTCAAG
    GAGGGGCGTC CTTTCTCTGG GGTGACTGCA TGCATGGATT TCTGTGCCCA TGCCCACAAA
    GACCAGCACA ATCTATACAA TGGATGCACT GTGGTCTGCA CTCTAACTAA GGAGGATAAC
    CGTATGATTG GCAGGGTTGC TGAGGATGAG CAGCTCCATG TACTTCCACT GTACAAGGTT
    TCCACAACTG ATGAATTTGG GAGTGAGGAG GGTCAACTTG AAAAGATAAA GAAAGGAGGA
    ATCCACGTGT TGAGCTCTTT TCCCCGGGAG GTACGCAAGC TTTCAGAACC AGCAAAGTCT
    TGCAGGCAGA GACAACTAGA AGCCAAGAAG GCCGCAGCCG AAAAGAAGAA ACTTCAAAAG
    GAGAAACTGG TCTCTCCGGA TAAGACCAAG CAGGAGCCAT CAGACAAAAA GACGTGCCAG
    CAAAACCCAG GTGTTCCTCA GCAACAGACA AAGCCTTGTA TAAAAGTGGA GCCTTCCAAT
    CACTACAATA ACTTTAAGTA CAATGGAAAT GGAGTTGTGG AGAGCTACTC AGTGTTGGGC
    AGCTGCAGGC CGTCCGACCC TTACAGCATG AACAGCGTTT ACTCCTATCA TTCTTTCTAT
    GCACAACCTA ACCTGCCTTC CGTGAATGGC TTTCATTCAA AATATGCTCT TCCTCCCTTT
    GGATTTTATG GCTTTCCTAA CAATCCTGTG GTTCCCAACC AGTTCATGAA TTATGGTACA
    AGTGATGCAA GGAACAGTGG ATGGATGAAT AATTGTTTTG AGAAAAAGCC AGAGCTTCAG
    TCACTGGCAG ATGGAATGAA TCAAAGTTAC GGGAGTGAGT TATCAGAGCA GAGTTTTAGG
    AGATCAAGTG AGGTACCACA CCACTACAGC CTCCAAAATC CCAGCAGTCA AAAATCAGTT
    AATGTTCCCC ACCGAACTAC TCCTGCACCA GTGGAGACTA CTCCATATTC TAATCTTCCC
    TGTTATAACA AAGTCATTAA AAAGGAGCCA GGTAGTGATC CACTTGTAGA TTCTTTCCAG
    AGAGCAAATA GTGTCCATTC ACATAGCCCA GGAGTTAATC ACTCATTACA GGCCAGTGAT
    CTGCCCATCT CATACAAAGC TAATGGTGCT CTATCATCAT CAGGGAGGAC TAATGCAGAA
    AGTCCGTGCA GTATGTTTAT GCCCAATGAT AAAAATGGTC TAGAAAAGAA AGATTATTTT
    GGAGTCCATT CGAATGCCCC TGGTCTGAAG GACAAGCAGT GGCCTCCCTA TGGAACTGAT
    GTTTCAGTGA GACAACATGA TTCACTTGAC TCGCAGTCTC CAGGAAAAGT GTGGAGCTCA
    TGCAAGCTGA GTGACAGTTC CGCTGCCCTG CCCTCCTCTG CAAGTACACA GGATAAGAAC
    TGGAATGGTA GACAGGTCAG TCTTAATCAG GGGATGAAGG AGTCTGCGCT GTTCCAAGAA
    AAACTCTGGA ATTCAGTTGC TGCCAGTGAC AGGTGCAGTG CCACGCCCAG TGACAGAAGT
    AGCATCACCC CCTGTTCTGA GCTTCAGGAC AAGAACTGGG GATCATTTCC TAATCCAACA
    GTAAACTCTT TGAAGACTGA CTCCAGCCAG AACCACTGGG ATCCCTATAG CCTAGATGAT
    AATATGGATG ATGGCCAATC AAAGAGTGTA AAAGAGGAGG ATGATGAGGA GATCTGGTCT
    GATAGTGAGC ACAACTTTCT GGATGAGAAT ATCGGAGGTG TGGCTGTAGC CCCAGGACAT
    GGCTCTATCC TAATTGAATG TGCAAGGAGA GAGCTCCATG CTACCACTCC ACTGAAAAAG
    CCCAACAGAT GTCATCCAAC TCGAATCTCT CTTGTATTCT ACCAACACAA GAACTTAAAC
    CAACCTAACC ATGGATTGGC ACTTTGGGAG GCCAAAATGA AGCAGTTGGC AGAAAGGGCA
    CGTGCAAGGG AGGAAGAGGC TGCCAAGCTA GGTATCAAAC AGGAAGTAAA AAGTTTGGGT
    AAAAAGAGGA AGTGGGGTGG TGCTGCCACT ACAGAAACCC CACCAGTTGA GAAGAAAGAC
    TATACCCCTA CCCGACAGGC AGCTACAATT TTAACAGATT CTGCTACCAC TTCATTCTCC
    TATGCCTATA CCAAGGTTAC GGGACCATAC AGTCGCTTTA TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53117
    Clone ID Related Accession (Same CDS sequence) XM_031897487.1 , XM_031897488.1 , XM_031897489.1 , XM_031897490.1
    Accession Version XM_031897489.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5325bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product methylcytosine dioxygenase tet3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGAAGCAG GGCCAGTTAA CCATGTTCAA GAAGGAAGGT TAAAGCAGGA ATGTGACTCT 
    ACCCTCCCAA GTAAGGGATG TGAGGATCTA GCAAACCAAT TATTAATGGA AGCAAATTCG
    TGGCTAAGCA ATACAGCTGC TCCTCAGGAT CCCTGCAACA AGCTCAACTG GGATAAACCC
    ACCATTCCCA ACCATGCTGC TAATAACAAT TCAAACCTAG AAGATGCCAA AAATCTAGTT
    GCATTCTCTG CTGTGGCTGA GGCTATGTCT ACCTATGGCA TGCCTGCATC AGGGACCCCC
    TCTTCGGTAT CACTGCAGCT ATATGAAAAG TTTAATTATG AGACAAACAG GGACAACTCT
    GGGCACTTGG AAGGCAATGC TCCATCATGT CCCGAAGACC TAAACACTCT AAAAGCAGCC
    CTTGCACTTG CAAAGCATGG CGTGAAACCA CCTAACTGTA ACTGTGACGG CCCTGAGTGC
    CCGGACTATC TAGAATGGCT GGAAAATAAA ATAAAATCTA CAGTTAAAGG TTCTCAGGAG
    AGCCCATTCC CTAATCTGGG TCAGGTCAGT AAAGAGCTGG TGCAAAAACA ATACCCTAAA
    GAACAGGTGC TAAATCTAGA AAATAAAAAT TCAACATGTC CTTCAGGTAA CCTTCCCTTT
    TCTCAAAATG CACTGAGTCT TGCTAAAGAA AAAAATATAA GTCTGCAAAC GGCCATTGCC
    ATTGAAGCTC TTACACAACT ATCATCTGCA CTTCCACAGA CAAACAATGA ATGCCCCAAT
    GCTCCTTCCC AACCTTTAAT AAACCCTCAT GATCAGTTAA CTCATTTCCC CTCTGCTAAA
    GGCAACCAGT TACCAATGTT ACCAGTGGCC CGCAATGAAC TGTTCCAGAA TCAGCAGTCC
    CAATTATACA CTGGCAAAAA TGCTCTTCCT GTGCCCCAAT CTCCAAGGCA AACTTCTTGG
    GAGCAGAACA AGAAATCTAG TTACCAAGAA GGACAATACA TTCCTGAAAA TTTATCACAT
    AGTTCTTCTG TGTTGCCCAG TGATGCTTCT ACCCCACAAA AACCTGAATT CCTTCAGCAG
    TGGGTCCAAA ATGCAGATCT TTTGAAGTCT CCATCTGATC CTATGACTGG GTTAAAGCAA
    TTGCTTGGGA ACACAGATGA GTACATCAAG TCTGTGTTTA AAGGGCCAGA GGCTTTGCCC
    AACAAAAAAA ATGTGAAGCC CAAACATACC ATTAAATCCA TAAAAAAAGA ATCCACCGAG
    TTTCTCAAAA TGTCACCAGA TCAACAGCTC TCTCAGCTTC TTCAAACAAA TGAATTTCAT
    CGTAATACCC AGGCTGCACT TCAGCAGCAT CTCCACCATA AACGCAATCT ATTTGTGGAC
    CCAAATGCTA TGGAAGCATG TACCCAAGAA CAGCAAAACT GGTGGGTGCC GAGTTCTCAA
    CAAGCTCCAG TTTCCAAGAC AACAGAGAAA CCTGTAAAAG AGAGGAAGAA AAGGCGACAG
    TCTCCTTCAC AAAAGCAAGT GGAACCAAAA CCGAAACCTC AACGTAAGCA AGTGCAAATA
    AAGAAACCCA AGGTGAAGGA AGGAAGTGCA GTCTTCATGC CTGTGAGCCA AATAAGCCTG
    GATACTTTTC GCAGAGTTGA AAAAGAAGAG AACCAGGGGA AAGAAATGGA TGCAGAGAAC
    TCTCTTCCTA ATAACGTACA AACAGAACTA CTTGAATCCC AGTCTTTACA ACTGACTGGA
    TCACAGGCAA ATCCTGATGA CAGAAAGACT GTAAACACTC AGGAAATGTG CAATGAAAAT
    CAGTCTAACA TTGGGAAGGC CAATAACTTT GCACTTTGTG TCAATCGGGC TAATTCTTTT
    GTTGCAAAAG ACCAATGTCC AACACCTTCC ACACATGATA CCAGCTCTAG CTCTGGACAA
    GGTGATTCTG CCAACCAGCA CCACACAAAT GTGAGTGATG TTCCTGGGCA AAATGACCTT
    AGCTGCCTTG ATGACAAGTT GGAAGATCTA ATAAGGCAGT TTGAAGCTGA GTTTGGGGAA
    GATTTCTCTC TACCTGGTTC TGCGGTCCCC TCTCAGAATG GTGAAGGACC GCCAAAACAA
    ACACCATCAG GAGATCCACA ATTTAAACTG CCCTTTCCTT CCCAGTTGTT ACCACCTGAG
    AATTCCACAA AGCCTGCCAC CCATTCCAAC CCTGCACTTT CAAATAATCC TGTATCACGT
    GAAGTTTCTA ATAACCTTGA TAGTCTGTTC TCATCAAAGT CTCCCAAGCA GATAAAAATC
    GAATCTTCTG GTGCTATAAC AGTGGTGTCT ACCACATGCT TCTACTCAGA GGAAAACCAA
    CATCTTGATG GAACTCCTAC AAAAAGTGAC CTGCCTTTCA ATCCTACTCT TAGTGGGTTT
    TTGGACTCGC CTCTCAAATA TCTGACATCT CCAACCAAGA GCTTAATAGA TACACCAGCT
    AAAAAGGCTC AAGCAGAATT TCCAACATGC GACTGTGTTG AACAAATTAA TGAAAAAGAT
    GAAGGCCCAT ATTATACACA TCTGGGTTCT GGACCAACAG TGGCTTCTAT CAGAGAGCTA
    ATGGAAGAGC GGTTTGGACA AAAAGGGGAT GCAATCCGCA TTGAAAAGGT GATCTACACA
    GGAAAAGAAG GAAAAAGCTC AAGGGGTTGT CCTATTGCAA AATGGGTCAT TCGCCGTCAG
    AGCGAGGATG AGAAGTTGAT GTGCCTTGTC CGGCAACGGG CAGGCCACCA TTGTGAAAAT
    GCAGTCATCA TTATCTTAAT TATGGCCTGG GAAGGAATTC CTCGCTCTCT AGGAGATTCT
    CTCTACAACG ACATCACTGA GACAATCACC AAGTATGGAA ATCCAACATC ACGGAGATGT
    GGACTGAATG ATGATCGAAC CTGTGCATGC CAAGGCAAGG ACCCAAATAC CTGTGGTGCT
    TCTTTCTCTT TTGGGTGCTC ATGGAGCATG TACTTTAATG GTTGCAAGTA TGCTCGTAGC
    AAGACCCCCC GGAAGTTCCG ACTTATAGGA GAAAATCCCA AGGAGGAAGA TGGCCTGAAG
    GATAACTTTC AGAATCTAGC TACAAAGGTT GCTCCAGTGT ACAAGATGCT TGCTCCTCAG
    GCTTATCAAA ATCAGGTTAA CAATGAGGAT ATTGCAATAG ATTGTCGGCT AGGTCTCAAG
    GAGGGGCGTC CTTTCTCTGG GGTGACTGCA TGCATGGATT TCTGTGCCCA TGCCCACAAA
    GACCAGCACA ATCTATACAA TGGATGCACT GTGGTCTGCA CTCTAACTAA GGAGGATAAC
    CGTATGATTG GCAGGGTTGC TGAGGATGAG CAGCTCCATG TACTTCCACT GTACAAGGTT
    TCCACAACTG ATGAATTTGG GAGTGAGGAG GGTCAACTTG AAAAGATAAA GAAAGGAGGA
    ATCCACGTGT TGAGCTCTTT TCCCCGGGAG GTACGCAAGC TTTCAGAACC AGCAAAGTCT
    TGCAGGCAGA GACAACTAGA AGCCAAGAAG GCCGCAGCCG AAAAGAAGAA ACTTCAAAAG
    GAGAAACTGG TCTCTCCGGA TAAGACCAAG CAGGAGCCAT CAGACAAAAA GACGTGCCAG
    CAAAACCCAG GTGTTCCTCA GCAACAGACA AAGCCTTGTA TAAAAGTGGA GCCTTCCAAT
    CACTACAATA ACTTTAAGTA CAATGGAAAT GGAGTTGTGG AGAGCTACTC AGTGTTGGGC
    AGCTGCAGGC CGTCCGACCC TTACAGCATG AACAGCGTTT ACTCCTATCA TTCTTTCTAT
    GCACAACCTA ACCTGCCTTC CGTGAATGGC TTTCATTCAA AATATGCTCT TCCTCCCTTT
    GGATTTTATG GCTTTCCTAA CAATCCTGTG GTTCCCAACC AGTTCATGAA TTATGGTACA
    AGTGATGCAA GGAACAGTGG ATGGATGAAT AATTGTTTTG AGAAAAAGCC AGAGCTTCAG
    TCACTGGCAG ATGGAATGAA TCAAAGTTAC GGGAGTGAGT TATCAGAGCA GAGTTTTAGG
    AGATCAAGTG AGGTACCACA CCACTACAGC CTCCAAAATC CCAGCAGTCA AAAATCAGTT
    AATGTTCCCC ACCGAACTAC TCCTGCACCA GTGGAGACTA CTCCATATTC TAATCTTCCC
    TGTTATAACA AAGTCATTAA AAAGGAGCCA GGTAGTGATC CACTTGTAGA TTCTTTCCAG
    AGAGCAAATA GTGTCCATTC ACATAGCCCA GGAGTTAATC ACTCATTACA GGCCAGTGAT
    CTGCCCATCT CATACAAAGC TAATGGTGCT CTATCATCAT CAGGGAGGAC TAATGCAGAA
    AGTCCGTGCA GTATGTTTAT GCCCAATGAT AAAAATGGTC TAGAAAAGAA AGATTATTTT
    GGAGTCCATT CGAATGCCCC TGGTCTGAAG GACAAGCAGT GGCCTCCCTA TGGAACTGAT
    GTTTCAGTGA GACAACATGA TTCACTTGAC TCGCAGTCTC CAGGAAAAGT GTGGAGCTCA
    TGCAAGCTGA GTGACAGTTC CGCTGCCCTG CCCTCCTCTG CAAGTACACA GGATAAGAAC
    TGGAATGGTA GACAGGTCAG TCTTAATCAG GGGATGAAGG AGTCTGCGCT GTTCCAAGAA
    AAACTCTGGA ATTCAGTTGC TGCCAGTGAC AGGTGCAGTG CCACGCCCAG TGACAGAAGT
    AGCATCACCC CCTGTTCTGA GCTTCAGGAC AAGAACTGGG GATCATTTCC TAATCCAACA
    GTAAACTCTT TGAAGACTGA CTCCAGCCAG AACCACTGGG ATCCCTATAG CCTAGATGAT
    AATATGGATG ATGGCCAATC AAAGAGTGTA AAAGAGGAGG ATGATGAGGA GATCTGGTCT
    GATAGTGAGC ACAACTTTCT GGATGAGAAT ATCGGAGGTG TGGCTGTAGC CCCAGGACAT
    GGCTCTATCC TAATTGAATG TGCAAGGAGA GAGCTCCATG CTACCACTCC ACTGAAAAAG
    CCCAACAGAT GTCATCCAAC TCGAATCTCT CTTGTATTCT ACCAACACAA GAACTTAAAC
    CAACCTAACC ATGGATTGGC ACTTTGGGAG GCCAAAATGA AGCAGTTGGC AGAAAGGGCA
    CGTGCAAGGG AGGAAGAGGC TGCCAAGCTA GGTATCAAAC AGGAAGTAAA AAGTTTGGGT
    AAAAAGAGGA AGTGGGGTGG TGCTGCCACT ACAGAAACCC CACCAGTTGA GAAGAAAGAC
    TATACCCCTA CCCGACAGGC AGCTACAATT TTAACAGATT CTGCTACCAC TTCATTCTCC
    TATGCCTATA CCAAGGTTAC GGGACCATAC AGTCGCTTTA TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753349.1
    CDS304..5628
    Translation

    Target ORF information:

    RefSeq Version XM_031897489.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis tet methylcytosine dioxygenase 3 (tet3), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897489.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGAAGCAG GGCCAGTTAA CCATGTTCAA GAAGGAAGGT TAAAGCAGGA ATGTGACTCT 
    ACCCTCCCAA GTAAGGGATG TGAGGATCTA GCAAACCAAT TATTAATGGA AGCAAATTCG
    TGGCTAAGCA ATACAGCTGC TCCTCAGGAT CCCTGCAACA AGCTCAACTG GGATAAACCC
    ACCATTCCCA ACCATGCTGC TAATAACAAT TCAAACCTAG AAGATGCCAA AAATCTAGTT
    GCATTCTCTG CTGTGGCTGA GGCTATGTCT ACCTATGGCA TGCCTGCATC AGGGACCCCC
    TCTTCGGTAT CACTGCAGCT ATATGAAAAG TTTAATTATG AGACAAACAG GGACAACTCT
    GGGCACTTGG AAGGCAATGC TCCATCATGT CCCGAAGACC TAAACACTCT AAAAGCAGCC
    CTTGCACTTG CAAAGCATGG CGTGAAACCA CCTAACTGTA ACTGTGACGG CCCTGAGTGC
    CCGGACTATC TAGAATGGCT GGAAAATAAA ATAAAATCTA CAGTTAAAGG TTCTCAGGAG
    AGCCCATTCC CTAATCTGGG TCAGGTCAGT AAAGAGCTGG TGCAAAAACA ATACCCTAAA
    GAACAGGTGC TAAATCTAGA AAATAAAAAT TCAACATGTC CTTCAGGTAA CCTTCCCTTT
    TCTCAAAATG CACTGAGTCT TGCTAAAGAA AAAAATATAA GTCTGCAAAC GGCCATTGCC
    ATTGAAGCTC TTACACAACT ATCATCTGCA CTTCCACAGA CAAACAATGA ATGCCCCAAT
    GCTCCTTCCC AACCTTTAAT AAACCCTCAT GATCAGTTAA CTCATTTCCC CTCTGCTAAA
    GGCAACCAGT TACCAATGTT ACCAGTGGCC CGCAATGAAC TGTTCCAGAA TCAGCAGTCC
    CAATTATACA CTGGCAAAAA TGCTCTTCCT GTGCCCCAAT CTCCAAGGCA AACTTCTTGG
    GAGCAGAACA AGAAATCTAG TTACCAAGAA GGACAATACA TTCCTGAAAA TTTATCACAT
    AGTTCTTCTG TGTTGCCCAG TGATGCTTCT ACCCCACAAA AACCTGAATT CCTTCAGCAG
    TGGGTCCAAA ATGCAGATCT TTTGAAGTCT CCATCTGATC CTATGACTGG GTTAAAGCAA
    TTGCTTGGGA ACACAGATGA GTACATCAAG TCTGTGTTTA AAGGGCCAGA GGCTTTGCCC
    AACAAAAAAA ATGTGAAGCC CAAACATACC ATTAAATCCA TAAAAAAAGA ATCCACCGAG
    TTTCTCAAAA TGTCACCAGA TCAACAGCTC TCTCAGCTTC TTCAAACAAA TGAATTTCAT
    CGTAATACCC AGGCTGCACT TCAGCAGCAT CTCCACCATA AACGCAATCT ATTTGTGGAC
    CCAAATGCTA TGGAAGCATG TACCCAAGAA CAGCAAAACT GGTGGGTGCC GAGTTCTCAA
    CAAGCTCCAG TTTCCAAGAC AACAGAGAAA CCTGTAAAAG AGAGGAAGAA AAGGCGACAG
    TCTCCTTCAC AAAAGCAAGT GGAACCAAAA CCGAAACCTC AACGTAAGCA AGTGCAAATA
    AAGAAACCCA AGGTGAAGGA AGGAAGTGCA GTCTTCATGC CTGTGAGCCA AATAAGCCTG
    GATACTTTTC GCAGAGTTGA AAAAGAAGAG AACCAGGGGA AAGAAATGGA TGCAGAGAAC
    TCTCTTCCTA ATAACGTACA AACAGAACTA CTTGAATCCC AGTCTTTACA ACTGACTGGA
    TCACAGGCAA ATCCTGATGA CAGAAAGACT GTAAACACTC AGGAAATGTG CAATGAAAAT
    CAGTCTAACA TTGGGAAGGC CAATAACTTT GCACTTTGTG TCAATCGGGC TAATTCTTTT
    GTTGCAAAAG ACCAATGTCC AACACCTTCC ACACATGATA CCAGCTCTAG CTCTGGACAA
    GGTGATTCTG CCAACCAGCA CCACACAAAT GTGAGTGATG TTCCTGGGCA AAATGACCTT
    AGCTGCCTTG ATGACAAGTT GGAAGATCTA ATAAGGCAGT TTGAAGCTGA GTTTGGGGAA
    GATTTCTCTC TACCTGGTTC TGCGGTCCCC TCTCAGAATG GTGAAGGACC GCCAAAACAA
    ACACCATCAG GAGATCCACA ATTTAAACTG CCCTTTCCTT CCCAGTTGTT ACCACCTGAG
    AATTCCACAA AGCCTGCCAC CCATTCCAAC CCTGCACTTT CAAATAATCC TGTATCACGT
    GAAGTTTCTA ATAACCTTGA TAGTCTGTTC TCATCAAAGT CTCCCAAGCA GATAAAAATC
    GAATCTTCTG GTGCTATAAC AGTGGTGTCT ACCACATGCT TCTACTCAGA GGAAAACCAA
    CATCTTGATG GAACTCCTAC AAAAAGTGAC CTGCCTTTCA ATCCTACTCT TAGTGGGTTT
    TTGGACTCGC CTCTCAAATA TCTGACATCT CCAACCAAGA GCTTAATAGA TACACCAGCT
    AAAAAGGCTC AAGCAGAATT TCCAACATGC GACTGTGTTG AACAAATTAA TGAAAAAGAT
    GAAGGCCCAT ATTATACACA TCTGGGTTCT GGACCAACAG TGGCTTCTAT CAGAGAGCTA
    ATGGAAGAGC GGTTTGGACA AAAAGGGGAT GCAATCCGCA TTGAAAAGGT GATCTACACA
    GGAAAAGAAG GAAAAAGCTC AAGGGGTTGT CCTATTGCAA AATGGGTCAT TCGCCGTCAG
    AGCGAGGATG AGAAGTTGAT GTGCCTTGTC CGGCAACGGG CAGGCCACCA TTGTGAAAAT
    GCAGTCATCA TTATCTTAAT TATGGCCTGG GAAGGAATTC CTCGCTCTCT AGGAGATTCT
    CTCTACAACG ACATCACTGA GACAATCACC AAGTATGGAA ATCCAACATC ACGGAGATGT
    GGACTGAATG ATGATCGAAC CTGTGCATGC CAAGGCAAGG ACCCAAATAC CTGTGGTGCT
    TCTTTCTCTT TTGGGTGCTC ATGGAGCATG TACTTTAATG GTTGCAAGTA TGCTCGTAGC
    AAGACCCCCC GGAAGTTCCG ACTTATAGGA GAAAATCCCA AGGAGGAAGA TGGCCTGAAG
    GATAACTTTC AGAATCTAGC TACAAAGGTT GCTCCAGTGT ACAAGATGCT TGCTCCTCAG
    GCTTATCAAA ATCAGGTTAA CAATGAGGAT ATTGCAATAG ATTGTCGGCT AGGTCTCAAG
    GAGGGGCGTC CTTTCTCTGG GGTGACTGCA TGCATGGATT TCTGTGCCCA TGCCCACAAA
    GACCAGCACA ATCTATACAA TGGATGCACT GTGGTCTGCA CTCTAACTAA GGAGGATAAC
    CGTATGATTG GCAGGGTTGC TGAGGATGAG CAGCTCCATG TACTTCCACT GTACAAGGTT
    TCCACAACTG ATGAATTTGG GAGTGAGGAG GGTCAACTTG AAAAGATAAA GAAAGGAGGA
    ATCCACGTGT TGAGCTCTTT TCCCCGGGAG GTACGCAAGC TTTCAGAACC AGCAAAGTCT
    TGCAGGCAGA GACAACTAGA AGCCAAGAAG GCCGCAGCCG AAAAGAAGAA ACTTCAAAAG
    GAGAAACTGG TCTCTCCGGA TAAGACCAAG CAGGAGCCAT CAGACAAAAA GACGTGCCAG
    CAAAACCCAG GTGTTCCTCA GCAACAGACA AAGCCTTGTA TAAAAGTGGA GCCTTCCAAT
    CACTACAATA ACTTTAAGTA CAATGGAAAT GGAGTTGTGG AGAGCTACTC AGTGTTGGGC
    AGCTGCAGGC CGTCCGACCC TTACAGCATG AACAGCGTTT ACTCCTATCA TTCTTTCTAT
    GCACAACCTA ACCTGCCTTC CGTGAATGGC TTTCATTCAA AATATGCTCT TCCTCCCTTT
    GGATTTTATG GCTTTCCTAA CAATCCTGTG GTTCCCAACC AGTTCATGAA TTATGGTACA
    AGTGATGCAA GGAACAGTGG ATGGATGAAT AATTGTTTTG AGAAAAAGCC AGAGCTTCAG
    TCACTGGCAG ATGGAATGAA TCAAAGTTAC GGGAGTGAGT TATCAGAGCA GAGTTTTAGG
    AGATCAAGTG AGGTACCACA CCACTACAGC CTCCAAAATC CCAGCAGTCA AAAATCAGTT
    AATGTTCCCC ACCGAACTAC TCCTGCACCA GTGGAGACTA CTCCATATTC TAATCTTCCC
    TGTTATAACA AAGTCATTAA AAAGGAGCCA GGTAGTGATC CACTTGTAGA TTCTTTCCAG
    AGAGCAAATA GTGTCCATTC ACATAGCCCA GGAGTTAATC ACTCATTACA GGCCAGTGAT
    CTGCCCATCT CATACAAAGC TAATGGTGCT CTATCATCAT CAGGGAGGAC TAATGCAGAA
    AGTCCGTGCA GTATGTTTAT GCCCAATGAT AAAAATGGTC TAGAAAAGAA AGATTATTTT
    GGAGTCCATT CGAATGCCCC TGGTCTGAAG GACAAGCAGT GGCCTCCCTA TGGAACTGAT
    GTTTCAGTGA GACAACATGA TTCACTTGAC TCGCAGTCTC CAGGAAAAGT GTGGAGCTCA
    TGCAAGCTGA GTGACAGTTC CGCTGCCCTG CCCTCCTCTG CAAGTACACA GGATAAGAAC
    TGGAATGGTA GACAGGTCAG TCTTAATCAG GGGATGAAGG AGTCTGCGCT GTTCCAAGAA
    AAACTCTGGA ATTCAGTTGC TGCCAGTGAC AGGTGCAGTG CCACGCCCAG TGACAGAAGT
    AGCATCACCC CCTGTTCTGA GCTTCAGGAC AAGAACTGGG GATCATTTCC TAATCCAACA
    GTAAACTCTT TGAAGACTGA CTCCAGCCAG AACCACTGGG ATCCCTATAG CCTAGATGAT
    AATATGGATG ATGGCCAATC AAAGAGTGTA AAAGAGGAGG ATGATGAGGA GATCTGGTCT
    GATAGTGAGC ACAACTTTCT GGATGAGAAT ATCGGAGGTG TGGCTGTAGC CCCAGGACAT
    GGCTCTATCC TAATTGAATG TGCAAGGAGA GAGCTCCATG CTACCACTCC ACTGAAAAAG
    CCCAACAGAT GTCATCCAAC TCGAATCTCT CTTGTATTCT ACCAACACAA GAACTTAAAC
    CAACCTAACC ATGGATTGGC ACTTTGGGAG GCCAAAATGA AGCAGTTGGC AGAAAGGGCA
    CGTGCAAGGG AGGAAGAGGC TGCCAAGCTA GGTATCAAAC AGGAAGTAAA AAGTTTGGGT
    AAAAAGAGGA AGTGGGGTGG TGCTGCCACT ACAGAAACCC CACCAGTTGA GAAGAAAGAC
    TATACCCCTA CCCGACAGGC AGCTACAATT TTAACAGATT CTGCTACCAC TTCATTCTCC
    TATGCCTATA CCAAGGTTAC GGGACCATAC AGTCGCTTTA TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53117
    Clone ID Related Accession (Same CDS sequence) XM_031897487.1 , XM_031897488.1 , XM_031897489.1 , XM_031897490.1
    Accession Version XM_031897490.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5325bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product methylcytosine dioxygenase tet3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGAAGCAG GGCCAGTTAA CCATGTTCAA GAAGGAAGGT TAAAGCAGGA ATGTGACTCT 
    ACCCTCCCAA GTAAGGGATG TGAGGATCTA GCAAACCAAT TATTAATGGA AGCAAATTCG
    TGGCTAAGCA ATACAGCTGC TCCTCAGGAT CCCTGCAACA AGCTCAACTG GGATAAACCC
    ACCATTCCCA ACCATGCTGC TAATAACAAT TCAAACCTAG AAGATGCCAA AAATCTAGTT
    GCATTCTCTG CTGTGGCTGA GGCTATGTCT ACCTATGGCA TGCCTGCATC AGGGACCCCC
    TCTTCGGTAT CACTGCAGCT ATATGAAAAG TTTAATTATG AGACAAACAG GGACAACTCT
    GGGCACTTGG AAGGCAATGC TCCATCATGT CCCGAAGACC TAAACACTCT AAAAGCAGCC
    CTTGCACTTG CAAAGCATGG CGTGAAACCA CCTAACTGTA ACTGTGACGG CCCTGAGTGC
    CCGGACTATC TAGAATGGCT GGAAAATAAA ATAAAATCTA CAGTTAAAGG TTCTCAGGAG
    AGCCCATTCC CTAATCTGGG TCAGGTCAGT AAAGAGCTGG TGCAAAAACA ATACCCTAAA
    GAACAGGTGC TAAATCTAGA AAATAAAAAT TCAACATGTC CTTCAGGTAA CCTTCCCTTT
    TCTCAAAATG CACTGAGTCT TGCTAAAGAA AAAAATATAA GTCTGCAAAC GGCCATTGCC
    ATTGAAGCTC TTACACAACT ATCATCTGCA CTTCCACAGA CAAACAATGA ATGCCCCAAT
    GCTCCTTCCC AACCTTTAAT AAACCCTCAT GATCAGTTAA CTCATTTCCC CTCTGCTAAA
    GGCAACCAGT TACCAATGTT ACCAGTGGCC CGCAATGAAC TGTTCCAGAA TCAGCAGTCC
    CAATTATACA CTGGCAAAAA TGCTCTTCCT GTGCCCCAAT CTCCAAGGCA AACTTCTTGG
    GAGCAGAACA AGAAATCTAG TTACCAAGAA GGACAATACA TTCCTGAAAA TTTATCACAT
    AGTTCTTCTG TGTTGCCCAG TGATGCTTCT ACCCCACAAA AACCTGAATT CCTTCAGCAG
    TGGGTCCAAA ATGCAGATCT TTTGAAGTCT CCATCTGATC CTATGACTGG GTTAAAGCAA
    TTGCTTGGGA ACACAGATGA GTACATCAAG TCTGTGTTTA AAGGGCCAGA GGCTTTGCCC
    AACAAAAAAA ATGTGAAGCC CAAACATACC ATTAAATCCA TAAAAAAAGA ATCCACCGAG
    TTTCTCAAAA TGTCACCAGA TCAACAGCTC TCTCAGCTTC TTCAAACAAA TGAATTTCAT
    CGTAATACCC AGGCTGCACT TCAGCAGCAT CTCCACCATA AACGCAATCT ATTTGTGGAC
    CCAAATGCTA TGGAAGCATG TACCCAAGAA CAGCAAAACT GGTGGGTGCC GAGTTCTCAA
    CAAGCTCCAG TTTCCAAGAC AACAGAGAAA CCTGTAAAAG AGAGGAAGAA AAGGCGACAG
    TCTCCTTCAC AAAAGCAAGT GGAACCAAAA CCGAAACCTC AACGTAAGCA AGTGCAAATA
    AAGAAACCCA AGGTGAAGGA AGGAAGTGCA GTCTTCATGC CTGTGAGCCA AATAAGCCTG
    GATACTTTTC GCAGAGTTGA AAAAGAAGAG AACCAGGGGA AAGAAATGGA TGCAGAGAAC
    TCTCTTCCTA ATAACGTACA AACAGAACTA CTTGAATCCC AGTCTTTACA ACTGACTGGA
    TCACAGGCAA ATCCTGATGA CAGAAAGACT GTAAACACTC AGGAAATGTG CAATGAAAAT
    CAGTCTAACA TTGGGAAGGC CAATAACTTT GCACTTTGTG TCAATCGGGC TAATTCTTTT
    GTTGCAAAAG ACCAATGTCC AACACCTTCC ACACATGATA CCAGCTCTAG CTCTGGACAA
    GGTGATTCTG CCAACCAGCA CCACACAAAT GTGAGTGATG TTCCTGGGCA AAATGACCTT
    AGCTGCCTTG ATGACAAGTT GGAAGATCTA ATAAGGCAGT TTGAAGCTGA GTTTGGGGAA
    GATTTCTCTC TACCTGGTTC TGCGGTCCCC TCTCAGAATG GTGAAGGACC GCCAAAACAA
    ACACCATCAG GAGATCCACA ATTTAAACTG CCCTTTCCTT CCCAGTTGTT ACCACCTGAG
    AATTCCACAA AGCCTGCCAC CCATTCCAAC CCTGCACTTT CAAATAATCC TGTATCACGT
    GAAGTTTCTA ATAACCTTGA TAGTCTGTTC TCATCAAAGT CTCCCAAGCA GATAAAAATC
    GAATCTTCTG GTGCTATAAC AGTGGTGTCT ACCACATGCT TCTACTCAGA GGAAAACCAA
    CATCTTGATG GAACTCCTAC AAAAAGTGAC CTGCCTTTCA ATCCTACTCT TAGTGGGTTT
    TTGGACTCGC CTCTCAAATA TCTGACATCT CCAACCAAGA GCTTAATAGA TACACCAGCT
    AAAAAGGCTC AAGCAGAATT TCCAACATGC GACTGTGTTG AACAAATTAA TGAAAAAGAT
    GAAGGCCCAT ATTATACACA TCTGGGTTCT GGACCAACAG TGGCTTCTAT CAGAGAGCTA
    ATGGAAGAGC GGTTTGGACA AAAAGGGGAT GCAATCCGCA TTGAAAAGGT GATCTACACA
    GGAAAAGAAG GAAAAAGCTC AAGGGGTTGT CCTATTGCAA AATGGGTCAT TCGCCGTCAG
    AGCGAGGATG AGAAGTTGAT GTGCCTTGTC CGGCAACGGG CAGGCCACCA TTGTGAAAAT
    GCAGTCATCA TTATCTTAAT TATGGCCTGG GAAGGAATTC CTCGCTCTCT AGGAGATTCT
    CTCTACAACG ACATCACTGA GACAATCACC AAGTATGGAA ATCCAACATC ACGGAGATGT
    GGACTGAATG ATGATCGAAC CTGTGCATGC CAAGGCAAGG ACCCAAATAC CTGTGGTGCT
    TCTTTCTCTT TTGGGTGCTC ATGGAGCATG TACTTTAATG GTTGCAAGTA TGCTCGTAGC
    AAGACCCCCC GGAAGTTCCG ACTTATAGGA GAAAATCCCA AGGAGGAAGA TGGCCTGAAG
    GATAACTTTC AGAATCTAGC TACAAAGGTT GCTCCAGTGT ACAAGATGCT TGCTCCTCAG
    GCTTATCAAA ATCAGGTTAA CAATGAGGAT ATTGCAATAG ATTGTCGGCT AGGTCTCAAG
    GAGGGGCGTC CTTTCTCTGG GGTGACTGCA TGCATGGATT TCTGTGCCCA TGCCCACAAA
    GACCAGCACA ATCTATACAA TGGATGCACT GTGGTCTGCA CTCTAACTAA GGAGGATAAC
    CGTATGATTG GCAGGGTTGC TGAGGATGAG CAGCTCCATG TACTTCCACT GTACAAGGTT
    TCCACAACTG ATGAATTTGG GAGTGAGGAG GGTCAACTTG AAAAGATAAA GAAAGGAGGA
    ATCCACGTGT TGAGCTCTTT TCCCCGGGAG GTACGCAAGC TTTCAGAACC AGCAAAGTCT
    TGCAGGCAGA GACAACTAGA AGCCAAGAAG GCCGCAGCCG AAAAGAAGAA ACTTCAAAAG
    GAGAAACTGG TCTCTCCGGA TAAGACCAAG CAGGAGCCAT CAGACAAAAA GACGTGCCAG
    CAAAACCCAG GTGTTCCTCA GCAACAGACA AAGCCTTGTA TAAAAGTGGA GCCTTCCAAT
    CACTACAATA ACTTTAAGTA CAATGGAAAT GGAGTTGTGG AGAGCTACTC AGTGTTGGGC
    AGCTGCAGGC CGTCCGACCC TTACAGCATG AACAGCGTTT ACTCCTATCA TTCTTTCTAT
    GCACAACCTA ACCTGCCTTC CGTGAATGGC TTTCATTCAA AATATGCTCT TCCTCCCTTT
    GGATTTTATG GCTTTCCTAA CAATCCTGTG GTTCCCAACC AGTTCATGAA TTATGGTACA
    AGTGATGCAA GGAACAGTGG ATGGATGAAT AATTGTTTTG AGAAAAAGCC AGAGCTTCAG
    TCACTGGCAG ATGGAATGAA TCAAAGTTAC GGGAGTGAGT TATCAGAGCA GAGTTTTAGG
    AGATCAAGTG AGGTACCACA CCACTACAGC CTCCAAAATC CCAGCAGTCA AAAATCAGTT
    AATGTTCCCC ACCGAACTAC TCCTGCACCA GTGGAGACTA CTCCATATTC TAATCTTCCC
    TGTTATAACA AAGTCATTAA AAAGGAGCCA GGTAGTGATC CACTTGTAGA TTCTTTCCAG
    AGAGCAAATA GTGTCCATTC ACATAGCCCA GGAGTTAATC ACTCATTACA GGCCAGTGAT
    CTGCCCATCT CATACAAAGC TAATGGTGCT CTATCATCAT CAGGGAGGAC TAATGCAGAA
    AGTCCGTGCA GTATGTTTAT GCCCAATGAT AAAAATGGTC TAGAAAAGAA AGATTATTTT
    GGAGTCCATT CGAATGCCCC TGGTCTGAAG GACAAGCAGT GGCCTCCCTA TGGAACTGAT
    GTTTCAGTGA GACAACATGA TTCACTTGAC TCGCAGTCTC CAGGAAAAGT GTGGAGCTCA
    TGCAAGCTGA GTGACAGTTC CGCTGCCCTG CCCTCCTCTG CAAGTACACA GGATAAGAAC
    TGGAATGGTA GACAGGTCAG TCTTAATCAG GGGATGAAGG AGTCTGCGCT GTTCCAAGAA
    AAACTCTGGA ATTCAGTTGC TGCCAGTGAC AGGTGCAGTG CCACGCCCAG TGACAGAAGT
    AGCATCACCC CCTGTTCTGA GCTTCAGGAC AAGAACTGGG GATCATTTCC TAATCCAACA
    GTAAACTCTT TGAAGACTGA CTCCAGCCAG AACCACTGGG ATCCCTATAG CCTAGATGAT
    AATATGGATG ATGGCCAATC AAAGAGTGTA AAAGAGGAGG ATGATGAGGA GATCTGGTCT
    GATAGTGAGC ACAACTTTCT GGATGAGAAT ATCGGAGGTG TGGCTGTAGC CCCAGGACAT
    GGCTCTATCC TAATTGAATG TGCAAGGAGA GAGCTCCATG CTACCACTCC ACTGAAAAAG
    CCCAACAGAT GTCATCCAAC TCGAATCTCT CTTGTATTCT ACCAACACAA GAACTTAAAC
    CAACCTAACC ATGGATTGGC ACTTTGGGAG GCCAAAATGA AGCAGTTGGC AGAAAGGGCA
    CGTGCAAGGG AGGAAGAGGC TGCCAAGCTA GGTATCAAAC AGGAAGTAAA AAGTTTGGGT
    AAAAAGAGGA AGTGGGGTGG TGCTGCCACT ACAGAAACCC CACCAGTTGA GAAGAAAGAC
    TATACCCCTA CCCGACAGGC AGCTACAATT TTAACAGATT CTGCTACCAC TTCATTCTCC
    TATGCCTATA CCAAGGTTAC GGGACCATAC AGTCGCTTTA TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753350.1
    CDS136..5460
    Translation

    Target ORF information:

    RefSeq Version XM_031897490.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis tet methylcytosine dioxygenase 3 (tet3), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897490.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGAAGCAG GGCCAGTTAA CCATGTTCAA GAAGGAAGGT TAAAGCAGGA ATGTGACTCT 
    ACCCTCCCAA GTAAGGGATG TGAGGATCTA GCAAACCAAT TATTAATGGA AGCAAATTCG
    TGGCTAAGCA ATACAGCTGC TCCTCAGGAT CCCTGCAACA AGCTCAACTG GGATAAACCC
    ACCATTCCCA ACCATGCTGC TAATAACAAT TCAAACCTAG AAGATGCCAA AAATCTAGTT
    GCATTCTCTG CTGTGGCTGA GGCTATGTCT ACCTATGGCA TGCCTGCATC AGGGACCCCC
    TCTTCGGTAT CACTGCAGCT ATATGAAAAG TTTAATTATG AGACAAACAG GGACAACTCT
    GGGCACTTGG AAGGCAATGC TCCATCATGT CCCGAAGACC TAAACACTCT AAAAGCAGCC
    CTTGCACTTG CAAAGCATGG CGTGAAACCA CCTAACTGTA ACTGTGACGG CCCTGAGTGC
    CCGGACTATC TAGAATGGCT GGAAAATAAA ATAAAATCTA CAGTTAAAGG TTCTCAGGAG
    AGCCCATTCC CTAATCTGGG TCAGGTCAGT AAAGAGCTGG TGCAAAAACA ATACCCTAAA
    GAACAGGTGC TAAATCTAGA AAATAAAAAT TCAACATGTC CTTCAGGTAA CCTTCCCTTT
    TCTCAAAATG CACTGAGTCT TGCTAAAGAA AAAAATATAA GTCTGCAAAC GGCCATTGCC
    ATTGAAGCTC TTACACAACT ATCATCTGCA CTTCCACAGA CAAACAATGA ATGCCCCAAT
    GCTCCTTCCC AACCTTTAAT AAACCCTCAT GATCAGTTAA CTCATTTCCC CTCTGCTAAA
    GGCAACCAGT TACCAATGTT ACCAGTGGCC CGCAATGAAC TGTTCCAGAA TCAGCAGTCC
    CAATTATACA CTGGCAAAAA TGCTCTTCCT GTGCCCCAAT CTCCAAGGCA AACTTCTTGG
    GAGCAGAACA AGAAATCTAG TTACCAAGAA GGACAATACA TTCCTGAAAA TTTATCACAT
    AGTTCTTCTG TGTTGCCCAG TGATGCTTCT ACCCCACAAA AACCTGAATT CCTTCAGCAG
    TGGGTCCAAA ATGCAGATCT TTTGAAGTCT CCATCTGATC CTATGACTGG GTTAAAGCAA
    TTGCTTGGGA ACACAGATGA GTACATCAAG TCTGTGTTTA AAGGGCCAGA GGCTTTGCCC
    AACAAAAAAA ATGTGAAGCC CAAACATACC ATTAAATCCA TAAAAAAAGA ATCCACCGAG
    TTTCTCAAAA TGTCACCAGA TCAACAGCTC TCTCAGCTTC TTCAAACAAA TGAATTTCAT
    CGTAATACCC AGGCTGCACT TCAGCAGCAT CTCCACCATA AACGCAATCT ATTTGTGGAC
    CCAAATGCTA TGGAAGCATG TACCCAAGAA CAGCAAAACT GGTGGGTGCC GAGTTCTCAA
    CAAGCTCCAG TTTCCAAGAC AACAGAGAAA CCTGTAAAAG AGAGGAAGAA AAGGCGACAG
    TCTCCTTCAC AAAAGCAAGT GGAACCAAAA CCGAAACCTC AACGTAAGCA AGTGCAAATA
    AAGAAACCCA AGGTGAAGGA AGGAAGTGCA GTCTTCATGC CTGTGAGCCA AATAAGCCTG
    GATACTTTTC GCAGAGTTGA AAAAGAAGAG AACCAGGGGA AAGAAATGGA TGCAGAGAAC
    TCTCTTCCTA ATAACGTACA AACAGAACTA CTTGAATCCC AGTCTTTACA ACTGACTGGA
    TCACAGGCAA ATCCTGATGA CAGAAAGACT GTAAACACTC AGGAAATGTG CAATGAAAAT
    CAGTCTAACA TTGGGAAGGC CAATAACTTT GCACTTTGTG TCAATCGGGC TAATTCTTTT
    GTTGCAAAAG ACCAATGTCC AACACCTTCC ACACATGATA CCAGCTCTAG CTCTGGACAA
    GGTGATTCTG CCAACCAGCA CCACACAAAT GTGAGTGATG TTCCTGGGCA AAATGACCTT
    AGCTGCCTTG ATGACAAGTT GGAAGATCTA ATAAGGCAGT TTGAAGCTGA GTTTGGGGAA
    GATTTCTCTC TACCTGGTTC TGCGGTCCCC TCTCAGAATG GTGAAGGACC GCCAAAACAA
    ACACCATCAG GAGATCCACA ATTTAAACTG CCCTTTCCTT CCCAGTTGTT ACCACCTGAG
    AATTCCACAA AGCCTGCCAC CCATTCCAAC CCTGCACTTT CAAATAATCC TGTATCACGT
    GAAGTTTCTA ATAACCTTGA TAGTCTGTTC TCATCAAAGT CTCCCAAGCA GATAAAAATC
    GAATCTTCTG GTGCTATAAC AGTGGTGTCT ACCACATGCT TCTACTCAGA GGAAAACCAA
    CATCTTGATG GAACTCCTAC AAAAAGTGAC CTGCCTTTCA ATCCTACTCT TAGTGGGTTT
    TTGGACTCGC CTCTCAAATA TCTGACATCT CCAACCAAGA GCTTAATAGA TACACCAGCT
    AAAAAGGCTC AAGCAGAATT TCCAACATGC GACTGTGTTG AACAAATTAA TGAAAAAGAT
    GAAGGCCCAT ATTATACACA TCTGGGTTCT GGACCAACAG TGGCTTCTAT CAGAGAGCTA
    ATGGAAGAGC GGTTTGGACA AAAAGGGGAT GCAATCCGCA TTGAAAAGGT GATCTACACA
    GGAAAAGAAG GAAAAAGCTC AAGGGGTTGT CCTATTGCAA AATGGGTCAT TCGCCGTCAG
    AGCGAGGATG AGAAGTTGAT GTGCCTTGTC CGGCAACGGG CAGGCCACCA TTGTGAAAAT
    GCAGTCATCA TTATCTTAAT TATGGCCTGG GAAGGAATTC CTCGCTCTCT AGGAGATTCT
    CTCTACAACG ACATCACTGA GACAATCACC AAGTATGGAA ATCCAACATC ACGGAGATGT
    GGACTGAATG ATGATCGAAC CTGTGCATGC CAAGGCAAGG ACCCAAATAC CTGTGGTGCT
    TCTTTCTCTT TTGGGTGCTC ATGGAGCATG TACTTTAATG GTTGCAAGTA TGCTCGTAGC
    AAGACCCCCC GGAAGTTCCG ACTTATAGGA GAAAATCCCA AGGAGGAAGA TGGCCTGAAG
    GATAACTTTC AGAATCTAGC TACAAAGGTT GCTCCAGTGT ACAAGATGCT TGCTCCTCAG
    GCTTATCAAA ATCAGGTTAA CAATGAGGAT ATTGCAATAG ATTGTCGGCT AGGTCTCAAG
    GAGGGGCGTC CTTTCTCTGG GGTGACTGCA TGCATGGATT TCTGTGCCCA TGCCCACAAA
    GACCAGCACA ATCTATACAA TGGATGCACT GTGGTCTGCA CTCTAACTAA GGAGGATAAC
    CGTATGATTG GCAGGGTTGC TGAGGATGAG CAGCTCCATG TACTTCCACT GTACAAGGTT
    TCCACAACTG ATGAATTTGG GAGTGAGGAG GGTCAACTTG AAAAGATAAA GAAAGGAGGA
    ATCCACGTGT TGAGCTCTTT TCCCCGGGAG GTACGCAAGC TTTCAGAACC AGCAAAGTCT
    TGCAGGCAGA GACAACTAGA AGCCAAGAAG GCCGCAGCCG AAAAGAAGAA ACTTCAAAAG
    GAGAAACTGG TCTCTCCGGA TAAGACCAAG CAGGAGCCAT CAGACAAAAA GACGTGCCAG
    CAAAACCCAG GTGTTCCTCA GCAACAGACA AAGCCTTGTA TAAAAGTGGA GCCTTCCAAT
    CACTACAATA ACTTTAAGTA CAATGGAAAT GGAGTTGTGG AGAGCTACTC AGTGTTGGGC
    AGCTGCAGGC CGTCCGACCC TTACAGCATG AACAGCGTTT ACTCCTATCA TTCTTTCTAT
    GCACAACCTA ACCTGCCTTC CGTGAATGGC TTTCATTCAA AATATGCTCT TCCTCCCTTT
    GGATTTTATG GCTTTCCTAA CAATCCTGTG GTTCCCAACC AGTTCATGAA TTATGGTACA
    AGTGATGCAA GGAACAGTGG ATGGATGAAT AATTGTTTTG AGAAAAAGCC AGAGCTTCAG
    TCACTGGCAG ATGGAATGAA TCAAAGTTAC GGGAGTGAGT TATCAGAGCA GAGTTTTAGG
    AGATCAAGTG AGGTACCACA CCACTACAGC CTCCAAAATC CCAGCAGTCA AAAATCAGTT
    AATGTTCCCC ACCGAACTAC TCCTGCACCA GTGGAGACTA CTCCATATTC TAATCTTCCC
    TGTTATAACA AAGTCATTAA AAAGGAGCCA GGTAGTGATC CACTTGTAGA TTCTTTCCAG
    AGAGCAAATA GTGTCCATTC ACATAGCCCA GGAGTTAATC ACTCATTACA GGCCAGTGAT
    CTGCCCATCT CATACAAAGC TAATGGTGCT CTATCATCAT CAGGGAGGAC TAATGCAGAA
    AGTCCGTGCA GTATGTTTAT GCCCAATGAT AAAAATGGTC TAGAAAAGAA AGATTATTTT
    GGAGTCCATT CGAATGCCCC TGGTCTGAAG GACAAGCAGT GGCCTCCCTA TGGAACTGAT
    GTTTCAGTGA GACAACATGA TTCACTTGAC TCGCAGTCTC CAGGAAAAGT GTGGAGCTCA
    TGCAAGCTGA GTGACAGTTC CGCTGCCCTG CCCTCCTCTG CAAGTACACA GGATAAGAAC
    TGGAATGGTA GACAGGTCAG TCTTAATCAG GGGATGAAGG AGTCTGCGCT GTTCCAAGAA
    AAACTCTGGA ATTCAGTTGC TGCCAGTGAC AGGTGCAGTG CCACGCCCAG TGACAGAAGT
    AGCATCACCC CCTGTTCTGA GCTTCAGGAC AAGAACTGGG GATCATTTCC TAATCCAACA
    GTAAACTCTT TGAAGACTGA CTCCAGCCAG AACCACTGGG ATCCCTATAG CCTAGATGAT
    AATATGGATG ATGGCCAATC AAAGAGTGTA AAAGAGGAGG ATGATGAGGA GATCTGGTCT
    GATAGTGAGC ACAACTTTCT GGATGAGAAT ATCGGAGGTG TGGCTGTAGC CCCAGGACAT
    GGCTCTATCC TAATTGAATG TGCAAGGAGA GAGCTCCATG CTACCACTCC ACTGAAAAAG
    CCCAACAGAT GTCATCCAAC TCGAATCTCT CTTGTATTCT ACCAACACAA GAACTTAAAC
    CAACCTAACC ATGGATTGGC ACTTTGGGAG GCCAAAATGA AGCAGTTGGC AGAAAGGGCA
    CGTGCAAGGG AGGAAGAGGC TGCCAAGCTA GGTATCAAAC AGGAAGTAAA AAGTTTGGGT
    AAAAAGAGGA AGTGGGGTGG TGCTGCCACT ACAGAAACCC CACCAGTTGA GAAGAAAGAC
    TATACCCCTA CCCGACAGGC AGCTACAATT TTAACAGATT CTGCTACCAC TTCATTCTCC
    TATGCCTATA CCAAGGTTAC GGGACCATAC AGTCGCTTTA TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa22183
    Clone ID Related Accession (Same CDS sequence) NM_001097187.1
    Accession Version NM_001097187.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5706bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product methylcytosine dioxygenase tet3
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC127289.1. ##Evidence-Data-START## Transcript exon combination :: BC127289.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028291, SAMD00028292 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGATACTC AGCCCGCGCC AGTTCCACAT GTGCTGCCCC AAGATGTATA TGAATTTCCT 
    GATGACCAAG AGTCCCTTGG GAGATTAAGA GTCTCAGAGA TGCCAGCGGA GCTGAATGGT
    GGTGGTGGTG GTGGCAGTGC AGCTGCCTTT GCCATGGAAC TTCCAGAGCA ATCTAATAAA
    AAAAGGAAGC GTTGTGGGGT CTGTGTGCCA TGTCTAAGGA AGGAACCATG TGGAGCCTGT
    TACAACTGTG TGAACCGGAG TACCAGCCAC CAGATATGCA AGATGCGAAA ATGCGAGCAG
    CTCAAAAAGA AAAGAGTGGT CCCCATGAAA GGAGTGGAGA ACTGTTCTGA AAGCATTTTA
    GTTGATGGAC CCAAAACAGA CCAAATGGAA GCAGGGCCAG TTAACCATGT TCAAGAAGGA
    AGGTTAAAGC AGGAATGTGA CTCTACCCTC CCAAGTAAGG GATGTGAGGA TCTAGCAAAC
    CAATTATTAA TGGAAGCAAA TTCGTGGCTA AGCAATACAG CTGCTCCTCA GGATCCCTGC
    AACAAGCTCA ACTGGGATAA ACCCACCATT CCCAACCATG CTGCTAATAA CAATTCAAAC
    CTAGAAGATG CCAAAAATCT AGTTGCATTC TCTGCTGTGG CTGAGGCTAT GTCTACCTAT
    GGCATGCCTG CATCAGGGAC CCCCTCTTCG GTATCACTGC AGCTATATGA AAAGTTTAAT
    TATGAGACAA ACAGGGACAA CTCTGGGCAC TTGGAAGGCA ATGCTCCATC ATGTCCCGAA
    GACCTAAACA CTCTAAAAGC AGCCCTTGCA CTTGCAAAGC ATGGCGTGAA ACCACCTAAC
    TGTAACTGTG ACGGCCCTGA GTGCCCGGAC TATCTAGAAT GGCTGGAAAA TAAAATAAAA
    TCTACAGTTA AAGGTTCTCA GGAGAGCCCA TTCCCTAATC TGGGTCAGGT TAGTAAAGAG
    CTGGTGCAAA AACAATACCC TAAAGAACAG GTGCTAAATC TAGAAAATAA AAATTCAACA
    TGTCCTTCAG GTAACCTTCC CTTTTCTCAA AATGCACTGA GTCTTGCTAA AGAAAAAAAT
    ATAAGTCTGC AAACGGCCAT TGCCATTGAA GCTCTTACAC AACTATCATC TGCACTTCCA
    CAGACAAACA ATGAATGCCC CAATGCTCCT TCCCAACCTT TAATAAACCC TCATGATCAG
    TTAACTCATT TCCCCTCTGC TAAAGGCAAC CAGTTACCAA TGTTACCAGT GGCCCGCAAT
    GAACTGTTCC AGAATCAGCA GTCCCAATTA TACACTGGCA AAAATGCTCT TCCTGTGCCC
    CAATCTCCAA GGCAAACTTC TTGGGAGCAG AACAAGAAAT CTAGTTACCA AGAAGGACAA
    TACATTCCTG AAAATTTATC ACATAGTTCT TCTGTGTTGC CCAGTGATGC TTCTACCCCA
    CAAAAACCTG AATTCCTTCA GCAGTGGGTC CAAAATGCAG ATCTTTTGAA GTCTCCATCT
    GATCCTATGA CTGGGTTAAA GCAATTGCTT GGGAACACAG ATGAGTACAT CAAGTCTGTG
    TTTAAAGGGC CAGAGGCTTT GCCCAACAAA AAAAATGTGA AGCCCAAACA TACCATTAAA
    TCCATAAAAA AAGAATCCAC CGAGTTTCTC AAAATGTCAC CAGATCAACA GCTCTCTCAG
    CTTCTTCAAA CAAATGAATT TCATCGTAAT ACCCAGGCTG CACTTCAGCA GCATCTCCAC
    CATAAACGCA ATCTATTTGT GGACCCAAAT GCTATGGAAG CATGTACCCA AGAACAGCAA
    AACTGGTGGG TGCCGAGTTC TCAACAAGCT CCAGTTTCCA AGACAACAGA GAAACCTGTA
    AAAGAGAGGA AGAAAAGGCG ACAGTCTCCT TCACAAAAGC AAGTGGAACC AAAACCGAAA
    CCTCAACGTA AGCAAGTGCA AATAAAGAAA CCCAAGGTGA AGGAAGGAAG TGCAGTCTTC
    ATGCCTGTGA GCCAAATAAG CCTGGATACT TTTCGCAGAG TGGAAAAAGA AGAGAACCAG
    GGGAAAGAAA TGGATGCAGA GAACTCTCTT CCTAATAACG TACAAACAGA ACTACTTGAA
    TCCCAGTCTT TACAACTGAC TGGATCACAG GCAAATCCTG ATGACAGAAA GACTGTAAAC
    ACTCAGGAAA TGTGCAATGA AAATCAGTCT AACATTGGGA AGGCCAATAA CTTTGCACTT
    TGTGTCAATC GGGCTAATTC TTTTGTTGCA AAAGACCAAT GTCCAACACC TTCCACACAT
    GATACCAGCT CTAGCTCTGG ACAAGGTGAT TCTGCCAACC AGCACACAAA TGTGAGTGAT
    GTTCCTGGGC AAAATGACCT TAGCTGCCTT GATGACAAGT TGGAAGATCT AATAAGGCAG
    TTTGAAGCTG AGTTTGGGGA AGATTTCTCT CTACCTGGTT CTGCGGTCCC CTCTCAGAAT
    GGTGAAGGAC CGCCAAAACA AACACCATCA GGAGATCCAC AATTTAAACT GCCCTTTCCT
    TCCCAGTTGT TACCACCTGA GAATTCCACA AAGCCTGCCA CCCATTCCAA CCCTGCACTT
    TCAAATAATC CTGTATCACG TGAAGTTTCT AATAACCTTG ATAGTTTGTT CTCATCAAAG
    TCTCCCAAGC AGATAAAAAT CGAATCTTCT GGTGCTATAA CAGTGGTGTC TACCACATGC
    TTCTACTCAG AGGAAAACCA ACATCTTGAT GGAACTCCTA CAAAAAGTGA CCTGCCTTTC
    AATCCTACTC TTAGTGGGTT TTTGGACTCG CCTCTCAAAT ATCTGACATC TCCAACCAAG
    AGCTTAATAG ATACACCAGC TAAAAAGGCT CAAGCAGAAT TTCCAACATG CGACTGTGTT
    GAACAAATTA ATGAAAAAGA TGAAGGCCCA TATTATACAC ATCTGGGTTC TGGACCAACA
    GTGGCTTCTA TCAGAGAGCT AATGGAAGAG CGGTTTGGAC AAAAAGGGGA TGCAATCCGC
    ATTGAAAAGG TGATCTACAC AGGAAAAGAA GGAAAAAGCT CAAGGGGTTG TCCTATTGCA
    AAATGGGTCA TTCGCCGTCA GAGCGAGGAT GAGAAGTTGA TGTGCCTTGT CCGGCAACGG
    GCAGGCCACC ATTGTGAAAA TGCAGTCATC ATTATCTTAA TTATGGCCTG GGAAGGAATT
    CCTCGCTCTC TAGGAGATTC TCTCTACAAC GACATCACTG AGACAATCAC CAAGTATGGA
    AATCCAACAT CACGGAGATG TGGACTGAAT GATGATCGAA CCTGTGCATG CCAAGGCAAG
    GACCCAAATA CCTGTGGTGC TTCTTTCTCT TTTGGGTGCT CATGGAGCAT GTACTTTAAT
    GGTTGCAAGT ATGCTCGTAG CAAGACCCCC CGGAAGTTCC GACTTATAGG AGAAAATCCC
    AAGGAGGAAG ATGGCCTGAA GGATAACTTT CAGAATCTAG CTACAAAGGT TGCTCCAGTG
    TACAAGATGC TTGCTCCTCA GGCTTATCAA AATCAGGTTA ACAATGAGGA TATTGCAATA
    GATTGTCGGC TAGGTCTCAA GGAGGGGCGT CCTTTCTCTG GGGTGACTGC ATGCATGGAT
    TTCTGTGCCC ATGCCCACAA AGACCAGCAC AATCTATACA ATGGATGCAC TGTGGTCTGC
    ACTCTAACTA AGGAGGATAA CCGTATGATT GGCAGGGTTG CTGAGGATGA GCAGCTCCAT
    GTACTTCCAC TGTACAAGGT TTCCACAACT GATGAATTTG GGAGTGAGGA GGGTCAACTT
    GAAAAGATAA AGAAAGGAGG AATCCACGTG TTGAGCTCTT TTCCCCGGGA GGTACGCAAG
    CTTTCAGAAC CAGCAAAGTC TTGCAGGCAG AGACAACTAG AAGCCAAGAA GGCCGCAGCC
    GAAAAGAAGA AACTTCAAAA GGAGAAACTG GTCTCTCCGG ATAAGACCAA GCAGGAGCCA
    TCAGACAAAA AGACGTGCCA GCAAAACCCA GGTGTTCCTC AGCAACAGAC AAAGCCTTGT
    ATAAAAGTGG AGCCTTCCAA TCACTACAAT AACTTTAAGT ACAATGGAAA TGGAGTTGTG
    GAGAGCTACT CAGTGTTGGG CAGCTGCAGG CCGTCCGACC CTTACAGCAT GAACAGCGTT
    TACTCCTATC ATTCTTTCTA TGCACAACCT AACCTGCCTT CCGTGAATGG CTTTCATTCA
    AAATATGCTC TTCCTCCCTT TGGATTTTAT GGCTTTCCTA ACAATCCTGT GGTTCCCAAC
    CAGTTCATGA ATTATGGTAC AAGTGATGCA AGGAACAGTG GATGGATGAA TAATTGTTTT
    GAGAAAAAGC CAGAGCTTCA GTCACTGGCA GATGGAATGA ATCAAAGTTA CGGGAGTGAG
    TTATCAGAGC AGAGTTTTAG GAGATCAAGT GAGGTACCAC ACCACTACAG CCTCCAAAAT
    CCCAGCAGTC AAAAATCAGT TAATGTTCCC CACCGAACTA CTCCTGCACC AGTGGAGACT
    ACTCCATATT CTAATCTTCC CTGTTATAAC AAAGTCATTA AAAAGGAGCC AGGTAGTGAT
    CCACTTGTAG ATTCTTTCCA GAGAGCAAAT AGTGTCCATT CACATAGCCC AGGAGTTAAT
    CACTCATTAC AGGCCAGTGA TCTGCCCATC TCATACAAAG CTAATGGTGC TCTATCATCA
    TCAGGGAGGA CTAATGCAGA AAGTCCGTGC AGTATGTTTA TGCCCAATGA TAAAAATGGT
    CTAGAAAAGA AAGATTATTT TGGAGTCCAT TCGAATGCCC CTGGTCTGAA GGACAAGCAG
    TGGCCTCCCT ATGGAACTGA TGTTTCAGTG AGACAACATG ATTCACTTGA CTCGCAGTCT
    CCAGGAAAAG TGTGGAGCTC ATGCAAGCTG AGTGACAGTT CCGCTGCCCT GCCCTCCTCT
    GCAAGTACAC AGGATAAGAA CTGGAATGGT AGACAGGTCA GTCTTAATCA GGGGATGAAG
    GAGTCTGCGC TGTTCCAAGA AAAACTCTGG AATTCAGTTG CTGCCAGTGA CAGGTGCAGT
    GCCACGCCCA GTGACAGAAG TAGCATCACC CCCTGTTCTG AGCTTCAGGA CAAGAACTGG
    GGATCATTTC CTAATCCAAC AGTAAACTCT TTGAAGACTG ACTCCAGCCA GAACCACTGG
    GATCCCTATA GCCTAGATGA TAATATGGAT GATGGCCAAT CAAAGAGTGT AAAAGAGGAG
    GATGATGAGG AGATCTGGTC TGATAGTGAG CACAACTTTC TGGATGAGAA TATCGGAGGT
    GTGGCTGTAG CCCCAGGACA TGGCTCTATC CTAATTGAAT GTGCAAGGAG AGAGCTCCAT
    GCTACCACTC CACTGAAAAA GCCCAACAGA TGTCATCCAA CTCGAATCTC TCTTGTATTC
    TACCAACACA AGAACTTAAA CCAACCTAAC CATGGATTGG CACTTTGGGA GGCCAAAATG
    AAGCAGTTGG CAGAAAGGGC ACGTGCAAGG GAGGAAGAGG CTGCCAAGCT AGGTATCAAA
    CAGGAAGTAA AAAGTTTGGG TAAAAAGAGG AAGTGGGGTG GTGCTGCCAC TACAGAAACC
    CCACCAGTTG AGAAGAAAGA CTATACCCCT ACCCGACAGG CAGCTACAAT TTTAACAGAT
    TCTGCTACCA CTTCATTCTC CTATGCCTAT ACCAAGGTTA CGGGACCATA CAGTCGCTTT
    ATATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001090656.1
    CDS442..6147
    Translation

    Target ORF information:

    RefSeq Version NM_001097187.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis tet methylcytosine dioxygenase 3 (tet3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001097187.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGATACTC AGCCCGCGCC AGTTCCACAT GTGCTGCCCC AAGATGTATA TGAATTTCCT 
    GATGACCAAG AGTCCCTTGG GAGATTAAGA GTCTCAGAGA TGCCAGCGGA GCTGAATGGT
    GGTGGTGGTG GTGGCAGTGC AGCTGCCTTT GCCATGGAAC TTCCAGAGCA ATCTAATAAA
    AAAAGGAAGC GTTGTGGGGT CTGTGTGCCA TGTCTAAGGA AGGAACCATG TGGAGCCTGT
    TACAACTGTG TGAACCGGAG TACCAGCCAC CAGATATGCA AGATGCGAAA ATGCGAGCAG
    CTCAAAAAGA AAAGAGTGGT CCCCATGAAA GGAGTGGAGA ACTGTTCTGA AAGCATTTTA
    GTTGATGGAC CCAAAACAGA CCAAATGGAA GCAGGGCCAG TTAACCATGT TCAAGAAGGA
    AGGTTAAAGC AGGAATGTGA CTCTACCCTC CCAAGTAAGG GATGTGAGGA TCTAGCAAAC
    CAATTATTAA TGGAAGCAAA TTCGTGGCTA AGCAATACAG CTGCTCCTCA GGATCCCTGC
    AACAAGCTCA ACTGGGATAA ACCCACCATT CCCAACCATG CTGCTAATAA CAATTCAAAC
    CTAGAAGATG CCAAAAATCT AGTTGCATTC TCTGCTGTGG CTGAGGCTAT GTCTACCTAT
    GGCATGCCTG CATCAGGGAC CCCCTCTTCG GTATCACTGC AGCTATATGA AAAGTTTAAT
    TATGAGACAA ACAGGGACAA CTCTGGGCAC TTGGAAGGCA ATGCTCCATC ATGTCCCGAA
    GACCTAAACA CTCTAAAAGC AGCCCTTGCA CTTGCAAAGC ATGGCGTGAA ACCACCTAAC
    TGTAACTGTG ACGGCCCTGA GTGCCCGGAC TATCTAGAAT GGCTGGAAAA TAAAATAAAA
    TCTACAGTTA AAGGTTCTCA GGAGAGCCCA TTCCCTAATC TGGGTCAGGT TAGTAAAGAG
    CTGGTGCAAA AACAATACCC TAAAGAACAG GTGCTAAATC TAGAAAATAA AAATTCAACA
    TGTCCTTCAG GTAACCTTCC CTTTTCTCAA AATGCACTGA GTCTTGCTAA AGAAAAAAAT
    ATAAGTCTGC AAACGGCCAT TGCCATTGAA GCTCTTACAC AACTATCATC TGCACTTCCA
    CAGACAAACA ATGAATGCCC CAATGCTCCT TCCCAACCTT TAATAAACCC TCATGATCAG
    TTAACTCATT TCCCCTCTGC TAAAGGCAAC CAGTTACCAA TGTTACCAGT GGCCCGCAAT
    GAACTGTTCC AGAATCAGCA GTCCCAATTA TACACTGGCA AAAATGCTCT TCCTGTGCCC
    CAATCTCCAA GGCAAACTTC TTGGGAGCAG AACAAGAAAT CTAGTTACCA AGAAGGACAA
    TACATTCCTG AAAATTTATC ACATAGTTCT TCTGTGTTGC CCAGTGATGC TTCTACCCCA
    CAAAAACCTG AATTCCTTCA GCAGTGGGTC CAAAATGCAG ATCTTTTGAA GTCTCCATCT
    GATCCTATGA CTGGGTTAAA GCAATTGCTT GGGAACACAG ATGAGTACAT CAAGTCTGTG
    TTTAAAGGGC CAGAGGCTTT GCCCAACAAA AAAAATGTGA AGCCCAAACA TACCATTAAA
    TCCATAAAAA AAGAATCCAC CGAGTTTCTC AAAATGTCAC CAGATCAACA GCTCTCTCAG
    CTTCTTCAAA CAAATGAATT TCATCGTAAT ACCCAGGCTG CACTTCAGCA GCATCTCCAC
    CATAAACGCA ATCTATTTGT GGACCCAAAT GCTATGGAAG CATGTACCCA AGAACAGCAA
    AACTGGTGGG TGCCGAGTTC TCAACAAGCT CCAGTTTCCA AGACAACAGA GAAACCTGTA
    AAAGAGAGGA AGAAAAGGCG ACAGTCTCCT TCACAAAAGC AAGTGGAACC AAAACCGAAA
    CCTCAACGTA AGCAAGTGCA AATAAAGAAA CCCAAGGTGA AGGAAGGAAG TGCAGTCTTC
    ATGCCTGTGA GCCAAATAAG CCTGGATACT TTTCGCAGAG TGGAAAAAGA AGAGAACCAG
    GGGAAAGAAA TGGATGCAGA GAACTCTCTT CCTAATAACG TACAAACAGA ACTACTTGAA
    TCCCAGTCTT TACAACTGAC TGGATCACAG GCAAATCCTG ATGACAGAAA GACTGTAAAC
    ACTCAGGAAA TGTGCAATGA AAATCAGTCT AACATTGGGA AGGCCAATAA CTTTGCACTT
    TGTGTCAATC GGGCTAATTC TTTTGTTGCA AAAGACCAAT GTCCAACACC TTCCACACAT
    GATACCAGCT CTAGCTCTGG ACAAGGTGAT TCTGCCAACC AGCACACAAA TGTGAGTGAT
    GTTCCTGGGC AAAATGACCT TAGCTGCCTT GATGACAAGT TGGAAGATCT AATAAGGCAG
    TTTGAAGCTG AGTTTGGGGA AGATTTCTCT CTACCTGGTT CTGCGGTCCC CTCTCAGAAT
    GGTGAAGGAC CGCCAAAACA AACACCATCA GGAGATCCAC AATTTAAACT GCCCTTTCCT
    TCCCAGTTGT TACCACCTGA GAATTCCACA AAGCCTGCCA CCCATTCCAA CCCTGCACTT
    TCAAATAATC CTGTATCACG TGAAGTTTCT AATAACCTTG ATAGTTTGTT CTCATCAAAG
    TCTCCCAAGC AGATAAAAAT CGAATCTTCT GGTGCTATAA CAGTGGTGTC TACCACATGC
    TTCTACTCAG AGGAAAACCA ACATCTTGAT GGAACTCCTA CAAAAAGTGA CCTGCCTTTC
    AATCCTACTC TTAGTGGGTT TTTGGACTCG CCTCTCAAAT ATCTGACATC TCCAACCAAG
    AGCTTAATAG ATACACCAGC TAAAAAGGCT CAAGCAGAAT TTCCAACATG CGACTGTGTT
    GAACAAATTA ATGAAAAAGA TGAAGGCCCA TATTATACAC ATCTGGGTTC TGGACCAACA
    GTGGCTTCTA TCAGAGAGCT AATGGAAGAG CGGTTTGGAC AAAAAGGGGA TGCAATCCGC
    ATTGAAAAGG TGATCTACAC AGGAAAAGAA GGAAAAAGCT CAAGGGGTTG TCCTATTGCA
    AAATGGGTCA TTCGCCGTCA GAGCGAGGAT GAGAAGTTGA TGTGCCTTGT CCGGCAACGG
    GCAGGCCACC ATTGTGAAAA TGCAGTCATC ATTATCTTAA TTATGGCCTG GGAAGGAATT
    CCTCGCTCTC TAGGAGATTC TCTCTACAAC GACATCACTG AGACAATCAC CAAGTATGGA
    AATCCAACAT CACGGAGATG TGGACTGAAT GATGATCGAA CCTGTGCATG CCAAGGCAAG
    GACCCAAATA CCTGTGGTGC TTCTTTCTCT TTTGGGTGCT CATGGAGCAT GTACTTTAAT
    GGTTGCAAGT ATGCTCGTAG CAAGACCCCC CGGAAGTTCC GACTTATAGG AGAAAATCCC
    AAGGAGGAAG ATGGCCTGAA GGATAACTTT CAGAATCTAG CTACAAAGGT TGCTCCAGTG
    TACAAGATGC TTGCTCCTCA GGCTTATCAA AATCAGGTTA ACAATGAGGA TATTGCAATA
    GATTGTCGGC TAGGTCTCAA GGAGGGGCGT CCTTTCTCTG GGGTGACTGC ATGCATGGAT
    TTCTGTGCCC ATGCCCACAA AGACCAGCAC AATCTATACA ATGGATGCAC TGTGGTCTGC
    ACTCTAACTA AGGAGGATAA CCGTATGATT GGCAGGGTTG CTGAGGATGA GCAGCTCCAT
    GTACTTCCAC TGTACAAGGT TTCCACAACT GATGAATTTG GGAGTGAGGA GGGTCAACTT
    GAAAAGATAA AGAAAGGAGG AATCCACGTG TTGAGCTCTT TTCCCCGGGA GGTACGCAAG
    CTTTCAGAAC CAGCAAAGTC TTGCAGGCAG AGACAACTAG AAGCCAAGAA GGCCGCAGCC
    GAAAAGAAGA AACTTCAAAA GGAGAAACTG GTCTCTCCGG ATAAGACCAA GCAGGAGCCA
    TCAGACAAAA AGACGTGCCA GCAAAACCCA GGTGTTCCTC AGCAACAGAC AAAGCCTTGT
    ATAAAAGTGG AGCCTTCCAA TCACTACAAT AACTTTAAGT ACAATGGAAA TGGAGTTGTG
    GAGAGCTACT CAGTGTTGGG CAGCTGCAGG CCGTCCGACC CTTACAGCAT GAACAGCGTT
    TACTCCTATC ATTCTTTCTA TGCACAACCT AACCTGCCTT CCGTGAATGG CTTTCATTCA
    AAATATGCTC TTCCTCCCTT TGGATTTTAT GGCTTTCCTA ACAATCCTGT GGTTCCCAAC
    CAGTTCATGA ATTATGGTAC AAGTGATGCA AGGAACAGTG GATGGATGAA TAATTGTTTT
    GAGAAAAAGC CAGAGCTTCA GTCACTGGCA GATGGAATGA ATCAAAGTTA CGGGAGTGAG
    TTATCAGAGC AGAGTTTTAG GAGATCAAGT GAGGTACCAC ACCACTACAG CCTCCAAAAT
    CCCAGCAGTC AAAAATCAGT TAATGTTCCC CACCGAACTA CTCCTGCACC AGTGGAGACT
    ACTCCATATT CTAATCTTCC CTGTTATAAC AAAGTCATTA AAAAGGAGCC AGGTAGTGAT
    CCACTTGTAG ATTCTTTCCA GAGAGCAAAT AGTGTCCATT CACATAGCCC AGGAGTTAAT
    CACTCATTAC AGGCCAGTGA TCTGCCCATC TCATACAAAG CTAATGGTGC TCTATCATCA
    TCAGGGAGGA CTAATGCAGA AAGTCCGTGC AGTATGTTTA TGCCCAATGA TAAAAATGGT
    CTAGAAAAGA AAGATTATTT TGGAGTCCAT TCGAATGCCC CTGGTCTGAA GGACAAGCAG
    TGGCCTCCCT ATGGAACTGA TGTTTCAGTG AGACAACATG ATTCACTTGA CTCGCAGTCT
    CCAGGAAAAG TGTGGAGCTC ATGCAAGCTG AGTGACAGTT CCGCTGCCCT GCCCTCCTCT
    GCAAGTACAC AGGATAAGAA CTGGAATGGT AGACAGGTCA GTCTTAATCA GGGGATGAAG
    GAGTCTGCGC TGTTCCAAGA AAAACTCTGG AATTCAGTTG CTGCCAGTGA CAGGTGCAGT
    GCCACGCCCA GTGACAGAAG TAGCATCACC CCCTGTTCTG AGCTTCAGGA CAAGAACTGG
    GGATCATTTC CTAATCCAAC AGTAAACTCT TTGAAGACTG ACTCCAGCCA GAACCACTGG
    GATCCCTATA GCCTAGATGA TAATATGGAT GATGGCCAAT CAAAGAGTGT AAAAGAGGAG
    GATGATGAGG AGATCTGGTC TGATAGTGAG CACAACTTTC TGGATGAGAA TATCGGAGGT
    GTGGCTGTAG CCCCAGGACA TGGCTCTATC CTAATTGAAT GTGCAAGGAG AGAGCTCCAT
    GCTACCACTC CACTGAAAAA GCCCAACAGA TGTCATCCAA CTCGAATCTC TCTTGTATTC
    TACCAACACA AGAACTTAAA CCAACCTAAC CATGGATTGG CACTTTGGGA GGCCAAAATG
    AAGCAGTTGG CAGAAAGGGC ACGTGCAAGG GAGGAAGAGG CTGCCAAGCT AGGTATCAAA
    CAGGAAGTAA AAAGTTTGGG TAAAAAGAGG AAGTGGGGTG GTGCTGCCAC TACAGAAACC
    CCACCAGTTG AGAAGAAAGA CTATACCCCT ACCCGACAGG CAGCTACAAT TTTAACAGAT
    TCTGCTACCA CTTCATTCTC CTATGCCTAT ACCAAGGTTA CGGGACCATA CAGTCGCTTT
    ATATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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