svil cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Gene Symbol	svil
Entrez Gene ID	100037851
Full Name	supervillin
General protein information	Preferred Names supervillin Names supervillin
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012965661.1	XP_012821115.1	supervillin isoform X5
XM_012965654.2	XP_012821108.1	supervillin isoform X1
XM_012965660.2	XP_012821114.1	supervillin isoform X4
XM_012965659.1	XP_012821113.1	supervillin isoform X3
XM_012965657.2	XP_012821111.1	supervillin isoform X2
XM_012965657.3	XP_012821111.1	supervillin isoform X2
XM_012965655.2	XP_012821109.1	supervillin isoform X3

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SVIL	NP_776615.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007181	XP_565017.3
Danio rerio (zebrafish)	svilb	XP_003197996.2
Xenopus tropicalis (tropical clawed frog)	svil	NP_001090765.1
Pan troglodytes (chimpanzee)	SVIL	XP_001136112.1
Canis lupus familiaris (dog)	SVIL	XP_005617106.1
Mus musculus (house mouse)	Svil	NP_694793.1
Rattus norvegicus (Norway rat)	Svil	NP_001101886.2
Gallus gallus (chicken)	SVIL	XP_004939254.1
	SVIL	XP_001083894.2
Danio rerio (zebrafish)	svila	NP_001030338.2
Homo sapiens (human)	SVIL	NP_068506.2
Drosophila melanogaster (fruit fly)	CG33232	NP_995968.1

Gene Ontology

The following svil gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the svil cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa29956	XM_012965660.2 Latest version!	Xenopus tropicalis supervillin (svil), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa29957	XM_012965661.1 Latest version!	Xenopus tropicalis supervillin (svil), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa29955	XM_012965657.3 Latest version!	Xenopus tropicalis supervillin (svil), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa29953	XM_012965654.2 Latest version!	Xenopus tropicalis supervillin (svil), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OXa29954	XM_012965659.1 Latest version!	Xenopus tropicalis supervillin (svil), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OXa29954	XM_012965655.2 Latest version!	Xenopus tropicalis supervillin (svil), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
View Old Accession Versions >>
OXa29955	XM_012965657.2	Xenopus tropicalis supervillin (svil), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

svil ( XM_012965660.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821114.1 Xenopus tropicalis supervillin (svil), transcript variant X5, mRNA.

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CloneID	OXa29956
Clone ID Related Accession (Same CDS sequence)	XM_012965660.2
Accession Version	XM_012965660.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5646bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	supervillin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965660.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGGG AAGCACAGTG CGTGTGCCCG GGTACGTGTC GGGGGCGCGC AGGCTGGTCT 
TGTGTTTTAC TGAAAAAGTC CCGTTTGGAT TTCTGCCTTA GAACTGACGT CACTTCAGAA 
GCAGGATTTG ATCGAGAAGG TACATTTCAA CACCGAACGT CACGTCTTCC ACAGAAAAAG 
CCGCCAAGTT TTCAGAAGAG TTTTGTTATT GAAGTTACTG GGGATTTCAC AAACAACTCC 
AGGGAAACTG ATGCACATCT GCTAGCTGTA CTTCCAAAAG TTTCAGAACT AAGGAAACTC 
TTTGAGCCAA GCAGGAGATC CATTTCAGAG ATGAAAAGAA AAGAAAGAAT AGCCAGACGT 
TTGGAAGGAA TTGATAACGA CCCACAACCA ATTATGCTAC AGACACGTTT ATTGGAAGAA 
GATATGCCAC GATATATGCG CGCAACAGAT GCATATAATC CACACATAGG TCATGCAAAT 
GAAGACACTG CCTCAGAGTC ATCAATAGAA ACACATTCTA GATGGCAAAA CCGCTGGCAG 
CCAAGCACTG AATCATCTTC TATGTCAGGT CAAATGGCGG TAGAGGAAAC AGAATCAAAA 
GCAGAAAGGA TAGCTCGATA CAAGGCGGAG CGAAGACGGC AATTGGCAGA AAGATATGGT 
GTCCCTATGG ATTCCGAAGC GGATTCCGAA TACCAGTCAA GGTACACGAG AATAAGGAAA 
GACGGTGAGA TCTCTGAAAA GAAATCTGTG TTAGAGGAAG ATGAAATTAA AGAGAATAGC 
TCAGATTATT TATACCGTCA AGACATTAAG GAGCCTCGCA GATTTCGAAT GTACGAATCA 
AAGCATCGGG ACATTAATGA GAATGTGTCG GAGAGAGATG CTCTCAAAAA TGTGGAAAAC 
AATAATAATC AATATCGAGA AATATCCGAG TTGGATGGAT CTACCTTTTC GTGCATGGGA 
CAAGCCATCA CAGTTGGGGA AGCACCCAGG TCTCCAAAGT TCCCTCGTCG TGCATCAGAG 
AAATCACTTC AGCTTTCCTC CTCTCCACCA TCTGAACAGC TACTGCTTAG CGACTCCAGA 
CAAGGATCAA GCTTCCAGTC ACCAGAGCAG ATTGCTGAAG AAGAAGCAAA AGTGGATGAA 
AGAGCCAAAC TTAGCGTGGC TGCGAAAAGG CTTCTATTCA GGGAAATGGA AAAATCTCTG 
GAGTCGAAAC CAGCCAAGCC ACGTGCCAGA AATGCAGCAG TGGAGAGGAG GCTTCGCCGA 
GCACAAGACA GGTCTCGGAC GCAACCCGTT ACCAGTGAAG AGGTGGTCAT TGCTGCTACT 
TTGCAGGCAT CATCCCATAA AAGCACAATA TCAAAGCAAG AGGAGATACA AAAGGAAATA 
CACGATGACC AGAGCATTCC CGAAGAGCCT GATAATTCCT CGCTTTCCTT GGCTGAAAAA 
ATGGCATTAT TTAATAAGCT TTCCCAGCCA ATAACAAAAG AGGTGTCTAC GAGGAGCAGA 
ATGGATATTC GACAAAGGCG AATGAATGCG CGTTACCAGA CTCAGCCCGT CACCCTTGTG 
GAGGTTGAAC AGTATAAGAA TGGAGGCGAA AAGATCGGCC CCTTATCTCA CAGTGTTGTA 
ACTTCCGTAG CCACCCTAAC CTCTCGGCCG TCTCCAGTTA GCCCTGGGGA TATGCATACT 
GCAAAGTCCT CCAGTTATGA AGAGAGAATA TCAAGTGTCT CCAGCACTTC AGCCCTGCTG 
CACACTTCAC TGGAAACAGG AAGCACTTCG GAAAGGAGCT TGCTGCTCTC GGAGCCCTGG 
AGTCCCCCAA GTGTGAAAGA TAAAGTATAT GAAAAGGAAC CGAGGAAACC AATGTTGGAA 
GAGACAAGGG TCTCTGCAGC AGAAGATAGC AGCAGCTTCG GAAAATGCAG CTCGCTCCAT 
AAGGAAAAAA CCCACCCCGC GCTTGATGTG GCAAAGGAGG AAGAACATGT TGAGGAGCAG 
AAAGTGCCTG GGCATGTTGC CTCAAGTAGC AGCAGAGTGG AGTTGCTCAG CCCCCAGAAG 
CTGTCCCAGC CTGCTGAGCA TTCAGAATCC CAAGCAAAGC CTGAAAGTGC CCTGCAGGTA 
GACGCTGAAG ATAAGAAGAG CCTACATCCC GAGCCCAGCA TGAGAAGAAG GAAAGAGATC 
CACGAGATTG TTGAGCCGGC CTCTGAACTC CTTGGCACCA GCGCTGCAAA GACCGTTTCT 
CACACTCCAG CTGCTGCTCC TGCTGCGGTG TCCTACGGAC CACAGGCATC AGAGCTAAGT 
GAGGAAAAGC CCGAGGAGGA AACAGCTGAG ATCTCCTCCA CCATCAGCAA CAGGTCCAAC 
GAGGATCTCG ATACGCCCGG CAAAGCCATG TCCATCAAAG ACAGGCTGGC GCTGCTAAAG 
AAGAGTGGGG AAGAGGATTG GAAAAACAGG CTTAACAAAA AGCAGGAGTA CAGCAAAGTG 
TCTGTCATCG AGCGCAGCAC GCTCACCCAG CTCCATGAGA TTGAGCAGTC GCTGACAAAG 
AAGCAAAGGG TGACTGACGG CAATGGGCTT CACGTGTCTA TTGAAGAAAG GAAGCAGCAG 
ATTACAGTCA GGGAGGAGGC CTGGAAAACC AAAGGCATAG GGGCAGCAAA TGATTCCACA 
CAATTTACAG TGGCCGGCCG CATGGTTAAG AAAGGTCTGG CCTCACCTAC AGCCATTACC 
CCCGTCGCAT CTCCAATGTC TCTCAAATTT AAAACTCCAG TTTCCAAGCC TCTGGAAGAA 
ATTGAAGCCA AACCTGATAT GAATTTGGAA TCTGACAGGA AGTTAGAAAA ACTAGAATCA 
TTTTTGGGAA AACTGCACAA CAGGGTTGGA GGGCTACAAG ATACCACACT GTCAGTCACA 
GGCCAATCAG TGAAGGAGGT GATGAAGGTG GACGATGATG AAACTTTCGG AAGGTTCTAC 
CGCCCTTTTG AATTCTCCAC TGCCCACGTG CCATTTGATT TTGAAGATGA TTTTGATCTT 
CTCTTCAGTT CGGAGGAACC AAAGCTGACA TCTGCTGTAG CAGAACACAA GCGTGCCGTC 
CGGCCAGCCC GCCGGGTGCA AGCGTCGGGA AACCCACTGA AGATGCTTGC GGCCAGGGAG 
GACATCCTAC AGGAGTACAC AGAGACGCGG CTGAACGTGG CGTTCATGGA GTCCAAGAGA 
ATGAAAGTAG AAAAAATGTC CAAGAACTCA AACTTCTCAG AAGTCGCACT TGCAGGCCTG 
GCAAGCAAAG AAAACTTTAC CAGCGTAAGC CTGCGGAGTG TCAATATGAC CGAGCAGCAC 
TCCAACAACA GCGCCGTGCC ATACAAGAAG CTTATGCTAG TTCAGATTAA AGGACGCAGG 
CACGTTCAGT CGAGGCTGGT GGAACCGCGT GCATCCTCTC TGAATAGCGG AGACTGCTTC 
CTGCTGATCA CCCCGCACCA CTGCTTCTTG TGGGTCGGAG AGTTTGCAAA CGTCATTGAG 
AAAGCCAAGG CTTCTGAGCT GGCATCAATA ATTCAGACCA AGAGGGAACT TGGATGCAGG 
GCATCGTATG TGCAAACAGT CGAGGAAGGA ATAAATACCC ACACGCATGG AGCCAAGGAT 
TTTTGGAAAC TTCTGAGCGG TCAGACTGAA TCTCAAGCTG CTGGGAGCCC AGAGGAGGAT 
GAGAGATATG AAAATGCCAT CATAGAAACA AACTGTATTT ATCGATTGCT GGAAGATAAG 
CTGGTCCCTG TTGATGAATT TTGGGGCAAA GTACCTAAGT GTTCACTTTT AGAGTCTAAA 
GAGGTGCTTG TGTTTGATTT TGGAAGTGAA GTGTATGTGT GGCATGGGAA GGAGGTGACC 
TTGGCACAGA GGAAGGTGGC GTTCCAGTTA GCCAAACATC TCTGGAATGG AGCTTTTGAC 
TATGAAAATT GTGATATCAA TCCTCTGGAC CCTGGGGAAT GCAATTCTCT CATTCCAAGG 
AAAGGGCACG GGCGCCCTGA TTGGGCAATA TTTGGAAGGC TGACAGAGCA TAATGAAACC 
ATACTGTTCA AGGAAAAGTT CCTGGATTGG ACAGAAATGA AGAAACCCAA TGAGAAGGGT 
GTGTGCGAGG ACTCACCGAA GCTTAAGGAA GACCCACAAT CTGATGTGAA GCCTTATGAC 
ATCATGCTGA TGGTCCCAGT TCCCCAGAAA TCTGTGGGTA CGGTTCTTGA TGGATTAAAT 
GTCGCCCGTG GTTATGGACT TATTGAGGGA GATGATTGCC GCCAGTATGA AATCTCAACC 
GTCTCGGTGG ATGTCTGGCA TATCCTTGAA TTCGACTACA GCCGGCTGCC GAAGCAGAGC 
ATTGGCCAGT TCCACGAGGG AGACGCCTAT GTGGTAAAAT GGAAGTACTT TGTGAGCACA 
ACAGTTGGAC AGCGCCAGAA AACCGAGCAC GTCCGGATAG CGGGTAAAGA GAAGTGCGCC 
TACTTCTTCT GGCAGGGACG GAACTCGCCC GTAAGTGAGA AGGGAACCTC AGCGCTGATG 
ACTGTGGAGC TTGATGAAGA GCGGGGAGCA CAGGTCCAGG TGCTTCAGGG AAAAGAGCCC 
CCCTGTTTCT TACAATGTTT CCAGGGAGGA ATGATTGTGC ATGCTGGGAG ACGGGAAGAA 
GAGGAAGAGA ACACGCAAAG TGACTGGCGG TTGTACTGTG TACGTGGGGA GGTGCCGGTG 
GAAGGGAATT TGATGGAAGT CGCTTGTCAC TGCAGCAGCC TGCGCTCCAG GACCTCCTTG 
ATACTCCTCA GTATAAATAA AGCGCTCATC TACCTTTGGC ACGGCTGCAA AGTCCAAATC 
CATGCCAAGG AAGTGGGACG GACGGCAGCC AATAAAATAA AAGAGGAGTG CCCCCTGGAA 
GCCGGGCTAC ACAGCAGCAG TAAGGTGACC ATATATGAAT GTGATGAAGG CTCGGAACCC 
CCCGGATTCT GGGATGCGCT GGGCAGGCGA GACCGTAAAG CGTACGACTG CATGTTGCAA 
GATCCTGGGA AATTTAACTT CACGCCACGA CTGTTCAGCC TCAGTAGTTC ATCGGGAGAG 
TTTACAGCAT CAGAAATCCT GTATCCTTCA AGGCATCCGA ATGTGGTTAA CTCCATGCCA 
TTTTTACAAG AAGACCTGTA TACGGCCTCC CAGCCAGCTT TATTCCTCGT TGACAACCAT 
CACGAGGTTT ACCTGTGGCA AGGTTGGTGG CCGTCTGAGA ATATTATTAC TGGGTCTGCT 
CGTATTCGGT GGGATTCAGA CAGAAAAAGC GCAATGGAGA CCGTACTCAG CTACTGCAAA 
GGAAAGAATA TCAAGAAACC TCCCAAGTCG TATCTAATCC ATGCCGGGCT GGAGCCTCTG 
ACCTTTACCA ACATGTTTCC AAGCTGGGAA CATAGGGAAG ACATTGCCCA AATCACAGAA 
AAGGATGCGG AAGTCTCTAA CCAGATAATC CTTGTAGAAG ATGTACTCGC TAAACTTTGT 
AAAAGAATCT ACCCCTTGGC AGATCTTCTT GCCCGCCCGT TGCCCGAAGG AGTCGACCCT 
CTCAAGCTTG AGCTCTATCT GTCAGATAAA GACTTTGAGG TTGCGTTAGA AATGACAAGA 
GAAGAATACA ACACACTGCC GCCCTGGAAA CAGGTTAATA CAAAGAAAGC CAAAGGACTT 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821114.1
CDS	12..5657
Translation	MSWEAQCVCPGTCRGRAGWSCVLLKKSRLDFCLRTDVTSEAGFDREGTFQHRTSRLPQKKPPSFQKSFVIEVTGDFTNNSRETDAHLLAVLPKVSELRKLFEPSRRSISEMKRKERIARRLEGIDNDPQPIMLQTRLLEEDMPRYMRATDAYNPHIGHANEDTASESSIETHSRWQNRWQPSTESSSMSGQMAVEETESKAERIARYKAERRRQLAERYGVPMDSEADSEYQSRYTRIRKDGEISEKKSVLEEDEIKENSSDYLYRQDIKEPRRFRMYESKHRDINENVSERDALKNVENNNNQYREISELDGSTFSCMGQAITVGEAPRSPKFPRRASEKSLQLSSSPPSEQLLLSDSRQGSSFQSPEQIAEEEAKVDERAKLSVAAKRLLFREMEKSLESKPAKPRARNAAVERRLRRAQDRSRTQPVTSEEVVIAATLQASSHKSTISKQEEIQKEIHDDQSIPEEPDNSSLSLAEKMALFNKLSQPITKEVSTRSRMDIRQRRMNARYQTQPVTLVEVEQYKNGGEKIGPLSHSVVTSVATLTSRPSPVSPGDMHTAKSSSYEERISSVSSTSALLHTSLETGSTSERSLLLSEPWSPPSVKDKVYEKEPRKPMLEETRVSAAEDSSSFGKCSSLHKEKTHPALDVAKEEEHVEEQKVPGHVASSSSRVELLSPQKLSQPAEHSESQAKPESALQVDAEDKKSLHPEPSMRRRKEIHEIVEPASELLGTSAAKTVSHTPAAAPAAVSYGPQASELSEEKPEEETAEISSTISNRSNEDLDTPGKAMSIKDRLALLKKSGEEDWKNRLNKKQEYSKVSVIERSTLTQLHEIEQSLTKKQRVTDGNGLHVSIEERKQQITVREEAWKTKGIGAANDSTQFTVAGRMVKKGLASPTAITPVASPMSLKFKTPVSKPLEEIEAKPDMNLESDRKLEKLESFLGKLHNRVGGLQDTTLSVTGQSVKEVMKVDDDETFGRFYRPFEFSTAHVPFDFEDDFDLLFSSEEPKLTSAVAEHKRAVRPARRVQASGNPLKMLAAREDILQEYTETRLNVAFMESKRMKVEKMSKNSNFSEVALAGLASKENFTSVSLRSVNMTEQHSNNSAVPYKKLMLVQIKGRRHVQSRLVEPRASSLNSGDCFLLITPHHCFLWVGEFANVIEKAKASELASIIQTKRELGCRASYVQTVEEGINTHTHGAKDFWKLLSGQTESQAAGSPEEDERYENAIIETNCIYRLLEDKLVPVDEFWGKVPKCSLLESKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGAFDYENCDINPLDPGECNSLIPRKGHGRPDWAIFGRLTEHNETILFKEKFLDWTEMKKPNEKGVCEDSPKLKEDPQSDVKPYDIMLMVPVPQKSVGTVLDGLNVARGYGLIEGDDCRQYEISTVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKYFVSTTVGQRQKTEHVRIAGKEKCAYFFWQGRNSPVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMIVHAGRREEEEENTQSDWRLYCVRGEVPVEGNLMEVACHCSSLRSRTSLILLSINKALIYLWHGCKVQIHAKEVGRTAANKIKEECPLEAGLHSSSKVTIYECDEGSEPPGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFTASEILYPSRHPNVVNSMPFLQEDLYTASQPALFLVDNHHEVYLWQGWWPSENIITGSARIRWDSDRKSAMETVLSYCKGKNIKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAQITEKDAEVSNQIILVEDVLAKLCKRIYPLADLLARPLPEGVDPLKLELYLSDKDFEVALEMTREEYNTLPPWKQVNTKKAKGLF

Target ORF information:

RefSeq Version	XM_012965660.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis supervillin (svil), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965660.2

ORF Insert Sequence:

ATGAGCTGGG AAGCACAGTG CGTGTGCCCG GGTACGTGTC GGGGGCGCGC AGGCTGGTCT 
TGTGTTTTAC TGAAAAAGTC CCGTTTGGAT TTCTGCCTTA GAACTGACGT CACTTCAGAA 
GCAGGATTTG ATCGAGAAGG TACATTTCAA CACCGAACGT CACGTCTTCC ACAGAAAAAG 
CCGCCAAGTT TTCAGAAGAG TTTTGTTATT GAAGTTACTG GGGATTTCAC AAACAACTCC 
AGGGAAACTG ATGCACATCT GCTAGCTGTA CTTCCAAAAG TTTCAGAACT AAGGAAACTC 
TTTGAGCCAA GCAGGAGATC CATTTCAGAG ATGAAAAGAA AAGAAAGAAT AGCCAGACGT 
TTGGAAGGAA TTGATAACGA CCCACAACCA ATTATGCTAC AGACACGTTT ATTGGAAGAA 
GATATGCCAC GATATATGCG CGCAACAGAT GCATATAATC CACACATAGG TCATGCAAAT 
GAAGACACTG CCTCAGAGTC ATCAATAGAA ACACATTCTA GATGGCAAAA CCGCTGGCAG 
CCAAGCACTG AATCATCTTC TATGTCAGGT CAAATGGCGG TAGAGGAAAC AGAATCAAAA 
GCAGAAAGGA TAGCTCGATA CAAGGCGGAG CGAAGACGGC AATTGGCAGA AAGATATGGT 
GTCCCTATGG ATTCCGAAGC GGATTCCGAA TACCAGTCAA GGTACACGAG AATAAGGAAA 
GACGGTGAGA TCTCTGAAAA GAAATCTGTG TTAGAGGAAG ATGAAATTAA AGAGAATAGC 
TCAGATTATT TATACCGTCA AGACATTAAG GAGCCTCGCA GATTTCGAAT GTACGAATCA 
AAGCATCGGG ACATTAATGA GAATGTGTCG GAGAGAGATG CTCTCAAAAA TGTGGAAAAC 
AATAATAATC AATATCGAGA AATATCCGAG TTGGATGGAT CTACCTTTTC GTGCATGGGA 
CAAGCCATCA CAGTTGGGGA AGCACCCAGG TCTCCAAAGT TCCCTCGTCG TGCATCAGAG 
AAATCACTTC AGCTTTCCTC CTCTCCACCA TCTGAACAGC TACTGCTTAG CGACTCCAGA 
CAAGGATCAA GCTTCCAGTC ACCAGAGCAG ATTGCTGAAG AAGAAGCAAA AGTGGATGAA 
AGAGCCAAAC TTAGCGTGGC TGCGAAAAGG CTTCTATTCA GGGAAATGGA AAAATCTCTG 
GAGTCGAAAC CAGCCAAGCC ACGTGCCAGA AATGCAGCAG TGGAGAGGAG GCTTCGCCGA 
GCACAAGACA GGTCTCGGAC GCAACCCGTT ACCAGTGAAG AGGTGGTCAT TGCTGCTACT 
TTGCAGGCAT CATCCCATAA AAGCACAATA TCAAAGCAAG AGGAGATACA AAAGGAAATA 
CACGATGACC AGAGCATTCC CGAAGAGCCT GATAATTCCT CGCTTTCCTT GGCTGAAAAA 
ATGGCATTAT TTAATAAGCT TTCCCAGCCA ATAACAAAAG AGGTGTCTAC GAGGAGCAGA 
ATGGATATTC GACAAAGGCG AATGAATGCG CGTTACCAGA CTCAGCCCGT CACCCTTGTG 
GAGGTTGAAC AGTATAAGAA TGGAGGCGAA AAGATCGGCC CCTTATCTCA CAGTGTTGTA 
ACTTCCGTAG CCACCCTAAC CTCTCGGCCG TCTCCAGTTA GCCCTGGGGA TATGCATACT 
GCAAAGTCCT CCAGTTATGA AGAGAGAATA TCAAGTGTCT CCAGCACTTC AGCCCTGCTG 
CACACTTCAC TGGAAACAGG AAGCACTTCG GAAAGGAGCT TGCTGCTCTC GGAGCCCTGG 
AGTCCCCCAA GTGTGAAAGA TAAAGTATAT GAAAAGGAAC CGAGGAAACC AATGTTGGAA 
GAGACAAGGG TCTCTGCAGC AGAAGATAGC AGCAGCTTCG GAAAATGCAG CTCGCTCCAT 
AAGGAAAAAA CCCACCCCGC GCTTGATGTG GCAAAGGAGG AAGAACATGT TGAGGAGCAG 
AAAGTGCCTG GGCATGTTGC CTCAAGTAGC AGCAGAGTGG AGTTGCTCAG CCCCCAGAAG 
CTGTCCCAGC CTGCTGAGCA TTCAGAATCC CAAGCAAAGC CTGAAAGTGC CCTGCAGGTA 
GACGCTGAAG ATAAGAAGAG CCTACATCCC GAGCCCAGCA TGAGAAGAAG GAAAGAGATC 
CACGAGATTG TTGAGCCGGC CTCTGAACTC CTTGGCACCA GCGCTGCAAA GACCGTTTCT 
CACACTCCAG CTGCTGCTCC TGCTGCGGTG TCCTACGGAC CACAGGCATC AGAGCTAAGT 
GAGGAAAAGC CCGAGGAGGA AACAGCTGAG ATCTCCTCCA CCATCAGCAA CAGGTCCAAC 
GAGGATCTCG ATACGCCCGG CAAAGCCATG TCCATCAAAG ACAGGCTGGC GCTGCTAAAG 
AAGAGTGGGG AAGAGGATTG GAAAAACAGG CTTAACAAAA AGCAGGAGTA CAGCAAAGTG 
TCTGTCATCG AGCGCAGCAC GCTCACCCAG CTCCATGAGA TTGAGCAGTC GCTGACAAAG 
AAGCAAAGGG TGACTGACGG CAATGGGCTT CACGTGTCTA TTGAAGAAAG GAAGCAGCAG 
ATTACAGTCA GGGAGGAGGC CTGGAAAACC AAAGGCATAG GGGCAGCAAA TGATTCCACA 
CAATTTACAG TGGCCGGCCG CATGGTTAAG AAAGGTCTGG CCTCACCTAC AGCCATTACC 
CCCGTCGCAT CTCCAATGTC TCTCAAATTT AAAACTCCAG TTTCCAAGCC TCTGGAAGAA 
ATTGAAGCCA AACCTGATAT GAATTTGGAA TCTGACAGGA AGTTAGAAAA ACTAGAATCA 
TTTTTGGGAA AACTGCACAA CAGGGTTGGA GGGCTACAAG ATACCACACT GTCAGTCACA 
GGCCAATCAG TGAAGGAGGT GATGAAGGTG GACGATGATG AAACTTTCGG AAGGTTCTAC 
CGCCCTTTTG AATTCTCCAC TGCCCACGTG CCATTTGATT TTGAAGATGA TTTTGATCTT 
CTCTTCAGTT CGGAGGAACC AAAGCTGACA TCTGCTGTAG CAGAACACAA GCGTGCCGTC 
CGGCCAGCCC GCCGGGTGCA AGCGTCGGGA AACCCACTGA AGATGCTTGC GGCCAGGGAG 
GACATCCTAC AGGAGTACAC AGAGACGCGG CTGAACGTGG CGTTCATGGA GTCCAAGAGA 
ATGAAAGTAG AAAAAATGTC CAAGAACTCA AACTTCTCAG AAGTCGCACT TGCAGGCCTG 
GCAAGCAAAG AAAACTTTAC CAGCGTAAGC CTGCGGAGTG TCAATATGAC CGAGCAGCAC 
TCCAACAACA GCGCCGTGCC ATACAAGAAG CTTATGCTAG TTCAGATTAA AGGACGCAGG 
CACGTTCAGT CGAGGCTGGT GGAACCGCGT GCATCCTCTC TGAATAGCGG AGACTGCTTC 
CTGCTGATCA CCCCGCACCA CTGCTTCTTG TGGGTCGGAG AGTTTGCAAA CGTCATTGAG 
AAAGCCAAGG CTTCTGAGCT GGCATCAATA ATTCAGACCA AGAGGGAACT TGGATGCAGG 
GCATCGTATG TGCAAACAGT CGAGGAAGGA ATAAATACCC ACACGCATGG AGCCAAGGAT 
TTTTGGAAAC TTCTGAGCGG TCAGACTGAA TCTCAAGCTG CTGGGAGCCC AGAGGAGGAT 
GAGAGATATG AAAATGCCAT CATAGAAACA AACTGTATTT ATCGATTGCT GGAAGATAAG 
CTGGTCCCTG TTGATGAATT TTGGGGCAAA GTACCTAAGT GTTCACTTTT AGAGTCTAAA 
GAGGTGCTTG TGTTTGATTT TGGAAGTGAA GTGTATGTGT GGCATGGGAA GGAGGTGACC 
TTGGCACAGA GGAAGGTGGC GTTCCAGTTA GCCAAACATC TCTGGAATGG AGCTTTTGAC 
TATGAAAATT GTGATATCAA TCCTCTGGAC CCTGGGGAAT GCAATTCTCT CATTCCAAGG 
AAAGGGCACG GGCGCCCTGA TTGGGCAATA TTTGGAAGGC TGACAGAGCA TAATGAAACC 
ATACTGTTCA AGGAAAAGTT CCTGGATTGG ACAGAAATGA AGAAACCCAA TGAGAAGGGT 
GTGTGCGAGG ACTCACCGAA GCTTAAGGAA GACCCACAAT CTGATGTGAA GCCTTATGAC 
ATCATGCTGA TGGTCCCAGT TCCCCAGAAA TCTGTGGGTA CGGTTCTTGA TGGATTAAAT 
GTCGCCCGTG GTTATGGACT TATTGAGGGA GATGATTGCC GCCAGTATGA AATCTCAACC 
GTCTCGGTGG ATGTCTGGCA TATCCTTGAA TTCGACTACA GCCGGCTGCC GAAGCAGAGC 
ATTGGCCAGT TCCACGAGGG AGACGCCTAT GTGGTAAAAT GGAAGTACTT TGTGAGCACA 
ACAGTTGGAC AGCGCCAGAA AACCGAGCAC GTCCGGATAG CGGGTAAAGA GAAGTGCGCC 
TACTTCTTCT GGCAGGGACG GAACTCGCCC GTAAGTGAGA AGGGAACCTC AGCGCTGATG 
ACTGTGGAGC TTGATGAAGA GCGGGGAGCA CAGGTCCAGG TGCTTCAGGG AAAAGAGCCC 
CCCTGTTTCT TACAATGTTT CCAGGGAGGA ATGATTGTGC ATGCTGGGAG ACGGGAAGAA 
GAGGAAGAGA ACACGCAAAG TGACTGGCGG TTGTACTGTG TACGTGGGGA GGTGCCGGTG 
GAAGGGAATT TGATGGAAGT CGCTTGTCAC TGCAGCAGCC TGCGCTCCAG GACCTCCTTG 
ATACTCCTCA GTATAAATAA AGCGCTCATC TACCTTTGGC ACGGCTGCAA AGTCCAAATC 
CATGCCAAGG AAGTGGGACG GACGGCAGCC AATAAAATAA AAGAGGAGTG CCCCCTGGAA 
GCCGGGCTAC ACAGCAGCAG TAAGGTGACC ATATATGAAT GTGATGAAGG CTCGGAACCC 
CCCGGATTCT GGGATGCGCT GGGCAGGCGA GACCGTAAAG CGTACGACTG CATGTTGCAA 
GATCCTGGGA AATTTAACTT CACGCCACGA CTGTTCAGCC TCAGTAGTTC ATCGGGAGAG 
TTTACAGCAT CAGAAATCCT GTATCCTTCA AGGCATCCGA ATGTGGTTAA CTCCATGCCA 
TTTTTACAAG AAGACCTGTA TACGGCCTCC CAGCCAGCTT TATTCCTCGT TGACAACCAT 
CACGAGGTTT ACCTGTGGCA AGGTTGGTGG CCGTCTGAGA ATATTATTAC TGGGTCTGCT 
CGTATTCGGT GGGATTCAGA CAGAAAAAGC GCAATGGAGA CCGTACTCAG CTACTGCAAA 
GGAAAGAATA TCAAGAAACC TCCCAAGTCG TATCTAATCC ATGCCGGGCT GGAGCCTCTG 
ACCTTTACCA ACATGTTTCC AAGCTGGGAA CATAGGGAAG ACATTGCCCA AATCACAGAA 
AAGGATGCGG AAGTCTCTAA CCAGATAATC CTTGTAGAAG ATGTACTCGC TAAACTTTGT 
AAAAGAATCT ACCCCTTGGC AGATCTTCTT GCCCGCCCGT TGCCCGAAGG AGTCGACCCT 
CTCAAGCTTG AGCTCTATCT GTCAGATAAA GACTTTGAGG TTGCGTTAGA AATGACAAGA 
GAAGAATACA ACACACTGCC GCCCTGGAAA CAGGTTAATA CAAAGAAAGC CAAAGGACTT 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

svil ( XM_012965661.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821115.1 Xenopus tropicalis supervillin (svil), transcript variant X6, mRNA.

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CloneID	OXa29957
Clone ID Related Accession (Same CDS sequence)	XM_012965661.1
Accession Version	XM_012965661.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	supervillin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT AGCCAGACGT TTGGAAGGAA TTGATAACGA CCCACAACCA 
ATTATGCTAC AGACACGTTT ATTGGAAGAA GATATGCCAC GATATATGCG CGCAACAGAT 
GCATATAATC CACACATAGG TCATGCAAAT GAAGACACTG CCTCAGAGTC ATCAATAGAA 
ACACATTCTA GATGGCAAAA CCGCTGGCAG CCAAGCACTG AATCATCTTC TATGTCAGGT 
CAAATGGCGG TAGAGGAAAC AGAATCAAAA GCAGAAAGGA TAGCTCGATA CAAGGCGGAG 
CGAAGACGGC AATTGGCAGA AAGATATGGT GTCCCTATGG ATTCCGAAGC GGATTCCGAA 
TACCAGTCAA GGTACACGAG AATAAGGAAA GACGGTGAGA TCTCTGAAAA GAAATCTGTG 
TTAGAGGAAG ATGAAATTAA AGAGAATAGC TCAGATTATT TATACCGTCA AGACATTAAG 
GAGCCTCGCA GATTTCGAAT GTACGAATCA AAGCATCGGG ACATTAATGA GAATGTGTCG 
GAGAGAGATG CTCTCAAAAA TGTGGAAAAC AATAATAATC AATATCGAGA AATATCCGAG 
TTGGATGGAT CTACCTTTTC GTGCATGGGA CAAGCCATCA CAGTTGGGGA AGCACCCAGG 
TCTCCAAAGT TCCCTCGTCG TGCATCAGAG AAATCACTTC AGCTTTCCTC CTCTCCACCA 
TCTGAACAGC TACTGCTTAG CGACTCCAGA CAAGGATCAA GCTTCCAGTC ACCAGAGCAG 
ATTGCTGAAG AAGAAGCAAA AGTGGATGAA AGAGCCAAAC TTAGCGTGGC TGCGAAAAGG 
CTTCTATTCA GGGAAATGGA AAAATCTCTG GAGTCGAAAC CAGCCAAGCC ACGTGCCAGA 
AATGCAGCAG TGGAGAGGAG GCTTCGCCGA GCACAAGACA GGTCTCGGAC GCAACCCGTT 
ACCAGTGAAG AGGTGGTCAT TGCTGCTACT TTGCAGGCAT CATCCCATAA AAGCACAATA 
TCAAAGCAAG AGGAGATACA AAAGGAAATA CACGATGACC AGAGCATTCC CGAAGAGCCT 
GATAATTCCT CGCTTTCCTT GGCTGAAAAA ATGGCATTAT TTAATAAGCT TTCCCAGCCA 
ATAACAAAAG AGGTGTCTAC GAGGAGCAGA ATGGATATTC GACAAAGGCG AATGAATGCG 
CGTTACCAGA CTCAGCCCGT CACCCTTGTG GAGGTTGAAC AGTATAAGAA TGGAGGCGAA 
AAGATCGGCC CCTTATCTCA CAGTGTTGTA ACTTCCGTAG CCACCCTAAC CTCTCGGCCG 
TCTCCAGTTA GCCCTGGGGA TATGCATACT GCAAAGTCCT CCAGTTATGA AGAGAGAATA 
TCAAGTGTCT CCAGCACTTC AGCCCTGCTG CACACTTCAC TGGAAACAGG AAGCACTTCG 
GAAAGGAGCT TGCTGCTCTC GGAGCCCTGG AGTCCCCCAA GTGTGAAAGA TAAAGTATAT 
GAAAAGGAAC CGAGGAAACC AATGTTGGAA GAGACAAGGG TCTCTGCAGC AGAAGATAGC 
AGCAGCTTCG GAAAATGCAG CTCGCTCCAT AAGGAAAAAA CCCACCCCGC GCTTGATGTG 
GCAAAGGAGG AAGAACATGT TGAGGAGCAG AAAGTGCCTG GGCATGTTGC CTCAAGTAGC 
AGCAGAGTGG AGTTGCTCAG CCCCCAGAAG CTGTCCCAGC CTGCTGAGCA TTCAGAATCC 
CAAGCAAAGC CTGAAAGTGC CCTGCAGGTA GACGCTGAAG ATAAGAAGAG CCTACATCCC 
GAGCCCAGCA TGAGAAGAAG GAAAGAGATC CACGAGATTG TTGAGCCGGC CTCTGAACTC 
CTTGGCACCA GCGCTGCAAA GACCGTTTCT CACACTCCAG CTGCTGCTCC TGCTGCGGTG 
TCCTACGGAC CACAGGCATC AGAGCTAAGT GAGGAAAAGC CCGAGGAGGA AACAGCTGAG 
ATCTCCTCCA CCATCAGCAA CAGGTCCAAC GAGGATCTCG ATACGCCCGG CAAAGCCATG 
TCCATCAAAG ACAGGCTGGC GCTGCTAAAG AAGAGTGGGG AAGAGGATTG GAAAAACAGG 
CTTAACAAAA AGCAGGAGTA CAGCAAAGTG TCTGTCATCG AGCGCAGCAC GCTCACCCAG 
CTCCATGAGA TTGAGCAGTC GCTGACAAAG AAGCAAAGGG TGACTGACGG CAATGGGCTT 
CACGTGTCTA TTGAAGAAAG GAAGCAGCAG ATTACAGTCA GGGAGGAGGC CTGGAAAACC 
AAAGGCATAG GGGCAGCAAA TGATTCCACA CAATTTACAG TGGCCGGCCG CATGGTTAAG 
AAAGGTCTGG CCTCACCTAC AGCCATTACC CCCGTCGCAT CTCCAATGTC TCTCAAATTT 
AAAACTCCAG TTTCCAAGCC TCTGGAAGAA ATTGAAGCCA AACCTGATAT GAATTTGGAA 
TCTGACAGGA AGTTAGAAAA ACTAGAATCA TTTTTGGGAA AACTGCACAA CAGGGTTGGA 
GGGCTACAAG ATACCACACT GTCAGTCACA GGCCAATCAG TGAAGGAGGT GATGAAGGTG 
GACGATGATG AAACTTTCGG AAGGTTCTAC CGCCCTTTTG AATTCTCCAC TGCCCACGTG 
CCATTTGATT TTGAAGATGA TTTTGATCTT CTCTTCAGTT CGGAGGAACC AAAGCTGACA 
TCTGCTGTAG CAGAACACAA GCGTGCCGTC CGGCCAGCCC GCCGGGTGCA AGCGTCGGGA 
AACCCACTGA AGATGCTTGC GGCCAGGGAG GACATCCTAC AGGAGTACAC AGAGACGCGG 
CTGAACGTGG CGTTCATGGA GTCCAAGAGA ATGAAAGTAG AAAAAATGTC CAAGAACTCA 
AACTTCTCAG AAGTCGCACT TGCAGGCCTG GCAAGCAAAG AAAACTTTAC CAGCGTAAGC 
CTGCGGAGTG TCAATATGAC CGAGCAGCAC TCCAACAACA GCGCCGTGCC ATACAAGAAG 
CTTATGCTAG TTCAGATTAA AGGACGCAGG CACGTTCAGT CGAGGCTGGT GGAACCGCGT 
GCATCCTCTC TGAATAGCGG AGACTGCTTC CTGCTGATCA CCCCGCACCA CTGCTTCTTG 
TGGGTCGGAG AGTTTGCAAA CGTCATTGAG AAAGCCAAGG CTTCTGAGCT GGCATCAATA 
ATTCAGACCA AGAGGGAACT TGGATGCAGG GCATCGTATG TGCAAACAGT CGAGGAAGGA 
ATAAATACCC ACACGCATGG AGCCAAGGAT TTTTGGAAAC TTCTGAGCGG TCAGACTGAA 
TCTCAAGCTG CTGGGAGCCC AGAGGAGGAT GAGAGATATG AAAATGCCAT CATAGAAACA 
AACTGTATTT ATCGATTGCT GGAAGATAAG CTGGTCCCTG TTGATGAATT TTGGGGCAAA 
GTACCTAAGT GTTCACTTTT AGAGTCTAAA GAGGTGCTTG TGTTTGATTT TGGAAGTGAA 
GTGTATGTGT GGCATGGGAA GGAGGTGACC TTGGCACAGA GGAAGGTGGC GTTCCAGTTA 
GCCAAACATC TCTGGAATGG AGCTTTTGAC TATGAAAATT GTGATATCAA TCCTCTGGAC 
CCTGGGGAAT GCAATTCTCT CATTCCAAGG AAAGGGCACG GGCGCCCTGA TTGGGCAATA 
TTTGGAAGGC TGACAGAGCA TAATGAAACC ATACTGTTCA AGGAAAAGTT CCTGGATTGG 
ACAGAAATGA AGAAACCCAA TGAGAAGGGT GTGTGCGAGG ACTCACCGAA GCTTAAGGAA 
GACCCACAAT CTGATGTGAA GCCTTATGAC ATCATGCTGA TGGTCCCAGT TCCCCAGAAA 
TCTGTGGGTA CGGTTCTTGA TGGATTAAAT GTCGCCCGTG GTTATGGACT TATTGAGGGA 
GATGATTGCC GCCAGTATGA AATCTCAACC GTCTCGGTGG ATGTCTGGCA TATCCTTGAA 
TTCGACTACA GCCGGCTGCC GAAGCAGAGC ATTGGCCAGT TCCACGAGGG AGACGCCTAT 
GTGGTAAAAT GGAAGTACTT TGTGAGCACA ACAGTTGGAC AGCGCCAGAA AACCGAGCAC 
GTCCGGATAG CGGGTAAAGA GAAGTGCGCC TACTTCTTCT GGCAGGGACG GAACTCGCCC 
GTAAGTGAGA AGGGAACCTC AGCGCTGATG ACTGTGGAGC TTGATGAAGA GCGGGGAGCA 
CAGGTCCAGG TGCTTCAGGG AAAAGAGCCC CCCTGTTTCT TACAATGTTT CCAGGGAGGA 
ATGATTGTGC ATGCTGGGAG ACGGGAAGAA GAGGAAGAGA ACACGCAAAG TGACTGGCGG 
TTGTACTGTG TACGTGGGGA GGTGCCGGTG GAAGGGAATT TGATGGAAGT CGCTTGTCAC 
TGCAGCAGCC TGCGCTCCAG GACCTCCTTG ATACTCCTCA GTATAAATAA AGCGCTCATC 
TACCTTTGGC ACGGCTGCAA AGTCCAAATC CATGCCAAGG AAGTGGGACG GACGGCAGCC 
AATAAAATAA AAGAGGAGTG CCCCCTGGAA GCCGGGCTAC ACAGCAGCAG TAAGGTGACC 
ATATATGAAT GTGATGAAGG CTCGGAACCC CCCGGATTCT GGGATGCGCT GGGCAGGCGA 
GACCGTAAAG CGTACGACTG CATGTTGCAA GATCCTGGGA AATTTAACTT CACGCCACGA 
CTGTTCAGCC TCAGTAGTTC ATCGGGAGAG TTTACAGCAT CAGAAATCCT GTATCCTTCA 
AGGCATCCGA ATGTGGTTAA CTCCATGCCA TTTTTACAAG AAGACCTGTA TACGGCCTCC 
CAGCCAGCTT TATTCCTCGT TGACAACCAT CACGAGGTTT ACCTGTGGCA AGGTTGGTGG 
CCGTCTGAGA ATATTATTAC TGGGTCTGCT CGTATTCGGT GGGATTCAGA CAGAAAAAGC 
GCAATGGAGA CCGTACTCAG CTACTGCAAA GGAAAGAATA TCAAGAAACC TCCCAAGTCG 
TATCTAATCC ATGCCGGGCT GGAGCCTCTG ACCTTTACCA ACATGTTTCC AAGCTGGGAA 
CATAGGGAAG ACATTGCCCA AATCACAGAA AAGGATGCGG AAGTCTCTAA CCAGATAATC 
CTTGTAGAAG ATGTACTCGC TAAACTTTGT AAAAGAATCT ACCCCTTGGC AGATCTTCTT 
GCCCGCCCGT TGCCCGAAGG AGTCGACCCT CTCAAGCTTG AGCTCTATCT GTCAGATAAA 
GACTTTGAGG TTGCGTTAGA AATGACAAGA GAAGAATACA ACACACTGCC GCCCTGGAAA 
CAGGTTAATA CAAAGAAAGC CAAAGGACTT TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821115.1
CDS	539..5854
Translation	MKRKERIARRLEGIDNDPQPIMLQTRLLEEDMPRYMRATDAYNPHIGHANEDTASESSIETHSRWQNRWQPSTESSSMSGQMAVEETESKAERIARYKAERRRQLAERYGVPMDSEADSEYQSRYTRIRKDGEISEKKSVLEEDEIKENSSDYLYRQDIKEPRRFRMYESKHRDINENVSERDALKNVENNNNQYREISELDGSTFSCMGQAITVGEAPRSPKFPRRASEKSLQLSSSPPSEQLLLSDSRQGSSFQSPEQIAEEEAKVDERAKLSVAAKRLLFREMEKSLESKPAKPRARNAAVERRLRRAQDRSRTQPVTSEEVVIAATLQASSHKSTISKQEEIQKEIHDDQSIPEEPDNSSLSLAEKMALFNKLSQPITKEVSTRSRMDIRQRRMNARYQTQPVTLVEVEQYKNGGEKIGPLSHSVVTSVATLTSRPSPVSPGDMHTAKSSSYEERISSVSSTSALLHTSLETGSTSERSLLLSEPWSPPSVKDKVYEKEPRKPMLEETRVSAAEDSSSFGKCSSLHKEKTHPALDVAKEEEHVEEQKVPGHVASSSSRVELLSPQKLSQPAEHSESQAKPESALQVDAEDKKSLHPEPSMRRRKEIHEIVEPASELLGTSAAKTVSHTPAAAPAAVSYGPQASELSEEKPEEETAEISSTISNRSNEDLDTPGKAMSIKDRLALLKKSGEEDWKNRLNKKQEYSKVSVIERSTLTQLHEIEQSLTKKQRVTDGNGLHVSIEERKQQITVREEAWKTKGIGAANDSTQFTVAGRMVKKGLASPTAITPVASPMSLKFKTPVSKPLEEIEAKPDMNLESDRKLEKLESFLGKLHNRVGGLQDTTLSVTGQSVKEVMKVDDDETFGRFYRPFEFSTAHVPFDFEDDFDLLFSSEEPKLTSAVAEHKRAVRPARRVQASGNPLKMLAAREDILQEYTETRLNVAFMESKRMKVEKMSKNSNFSEVALAGLASKENFTSVSLRSVNMTEQHSNNSAVPYKKLMLVQIKGRRHVQSRLVEPRASSLNSGDCFLLITPHHCFLWVGEFANVIEKAKASELASIIQTKRELGCRASYVQTVEEGINTHTHGAKDFWKLLSGQTESQAAGSPEEDERYENAIIETNCIYRLLEDKLVPVDEFWGKVPKCSLLESKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGAFDYENCDINPLDPGECNSLIPRKGHGRPDWAIFGRLTEHNETILFKEKFLDWTEMKKPNEKGVCEDSPKLKEDPQSDVKPYDIMLMVPVPQKSVGTVLDGLNVARGYGLIEGDDCRQYEISTVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKYFVSTTVGQRQKTEHVRIAGKEKCAYFFWQGRNSPVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMIVHAGRREEEEENTQSDWRLYCVRGEVPVEGNLMEVACHCSSLRSRTSLILLSINKALIYLWHGCKVQIHAKEVGRTAANKIKEECPLEAGLHSSSKVTIYECDEGSEPPGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFTASEILYPSRHPNVVNSMPFLQEDLYTASQPALFLVDNHHEVYLWQGWWPSENIITGSARIRWDSDRKSAMETVLSYCKGKNIKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAQITEKDAEVSNQIILVEDVLAKLCKRIYPLADLLARPLPEGVDPLKLELYLSDKDFEVALEMTREEYNTLPPWKQVNTKKAKGLF

Target ORF information:

RefSeq Version	XM_012965661.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis supervillin (svil), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965661.1

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT AGCCAGACGT TTGGAAGGAA TTGATAACGA CCCACAACCA 
ATTATGCTAC AGACACGTTT ATTGGAAGAA GATATGCCAC GATATATGCG CGCAACAGAT 
GCATATAATC CACACATAGG TCATGCAAAT GAAGACACTG CCTCAGAGTC ATCAATAGAA 
ACACATTCTA GATGGCAAAA CCGCTGGCAG CCAAGCACTG AATCATCTTC TATGTCAGGT 
CAAATGGCGG TAGAGGAAAC AGAATCAAAA GCAGAAAGGA TAGCTCGATA CAAGGCGGAG 
CGAAGACGGC AATTGGCAGA AAGATATGGT GTCCCTATGG ATTCCGAAGC GGATTCCGAA 
TACCAGTCAA GGTACACGAG AATAAGGAAA GACGGTGAGA TCTCTGAAAA GAAATCTGTG 
TTAGAGGAAG ATGAAATTAA AGAGAATAGC TCAGATTATT TATACCGTCA AGACATTAAG 
GAGCCTCGCA GATTTCGAAT GTACGAATCA AAGCATCGGG ACATTAATGA GAATGTGTCG 
GAGAGAGATG CTCTCAAAAA TGTGGAAAAC AATAATAATC AATATCGAGA AATATCCGAG 
TTGGATGGAT CTACCTTTTC GTGCATGGGA CAAGCCATCA CAGTTGGGGA AGCACCCAGG 
TCTCCAAAGT TCCCTCGTCG TGCATCAGAG AAATCACTTC AGCTTTCCTC CTCTCCACCA 
TCTGAACAGC TACTGCTTAG CGACTCCAGA CAAGGATCAA GCTTCCAGTC ACCAGAGCAG 
ATTGCTGAAG AAGAAGCAAA AGTGGATGAA AGAGCCAAAC TTAGCGTGGC TGCGAAAAGG 
CTTCTATTCA GGGAAATGGA AAAATCTCTG GAGTCGAAAC CAGCCAAGCC ACGTGCCAGA 
AATGCAGCAG TGGAGAGGAG GCTTCGCCGA GCACAAGACA GGTCTCGGAC GCAACCCGTT 
ACCAGTGAAG AGGTGGTCAT TGCTGCTACT TTGCAGGCAT CATCCCATAA AAGCACAATA 
TCAAAGCAAG AGGAGATACA AAAGGAAATA CACGATGACC AGAGCATTCC CGAAGAGCCT 
GATAATTCCT CGCTTTCCTT GGCTGAAAAA ATGGCATTAT TTAATAAGCT TTCCCAGCCA 
ATAACAAAAG AGGTGTCTAC GAGGAGCAGA ATGGATATTC GACAAAGGCG AATGAATGCG 
CGTTACCAGA CTCAGCCCGT CACCCTTGTG GAGGTTGAAC AGTATAAGAA TGGAGGCGAA 
AAGATCGGCC CCTTATCTCA CAGTGTTGTA ACTTCCGTAG CCACCCTAAC CTCTCGGCCG 
TCTCCAGTTA GCCCTGGGGA TATGCATACT GCAAAGTCCT CCAGTTATGA AGAGAGAATA 
TCAAGTGTCT CCAGCACTTC AGCCCTGCTG CACACTTCAC TGGAAACAGG AAGCACTTCG 
GAAAGGAGCT TGCTGCTCTC GGAGCCCTGG AGTCCCCCAA GTGTGAAAGA TAAAGTATAT 
GAAAAGGAAC CGAGGAAACC AATGTTGGAA GAGACAAGGG TCTCTGCAGC AGAAGATAGC 
AGCAGCTTCG GAAAATGCAG CTCGCTCCAT AAGGAAAAAA CCCACCCCGC GCTTGATGTG 
GCAAAGGAGG AAGAACATGT TGAGGAGCAG AAAGTGCCTG GGCATGTTGC CTCAAGTAGC 
AGCAGAGTGG AGTTGCTCAG CCCCCAGAAG CTGTCCCAGC CTGCTGAGCA TTCAGAATCC 
CAAGCAAAGC CTGAAAGTGC CCTGCAGGTA GACGCTGAAG ATAAGAAGAG CCTACATCCC 
GAGCCCAGCA TGAGAAGAAG GAAAGAGATC CACGAGATTG TTGAGCCGGC CTCTGAACTC 
CTTGGCACCA GCGCTGCAAA GACCGTTTCT CACACTCCAG CTGCTGCTCC TGCTGCGGTG 
TCCTACGGAC CACAGGCATC AGAGCTAAGT GAGGAAAAGC CCGAGGAGGA AACAGCTGAG 
ATCTCCTCCA CCATCAGCAA CAGGTCCAAC GAGGATCTCG ATACGCCCGG CAAAGCCATG 
TCCATCAAAG ACAGGCTGGC GCTGCTAAAG AAGAGTGGGG AAGAGGATTG GAAAAACAGG 
CTTAACAAAA AGCAGGAGTA CAGCAAAGTG TCTGTCATCG AGCGCAGCAC GCTCACCCAG 
CTCCATGAGA TTGAGCAGTC GCTGACAAAG AAGCAAAGGG TGACTGACGG CAATGGGCTT 
CACGTGTCTA TTGAAGAAAG GAAGCAGCAG ATTACAGTCA GGGAGGAGGC CTGGAAAACC 
AAAGGCATAG GGGCAGCAAA TGATTCCACA CAATTTACAG TGGCCGGCCG CATGGTTAAG 
AAAGGTCTGG CCTCACCTAC AGCCATTACC CCCGTCGCAT CTCCAATGTC TCTCAAATTT 
AAAACTCCAG TTTCCAAGCC TCTGGAAGAA ATTGAAGCCA AACCTGATAT GAATTTGGAA 
TCTGACAGGA AGTTAGAAAA ACTAGAATCA TTTTTGGGAA AACTGCACAA CAGGGTTGGA 
GGGCTACAAG ATACCACACT GTCAGTCACA GGCCAATCAG TGAAGGAGGT GATGAAGGTG 
GACGATGATG AAACTTTCGG AAGGTTCTAC CGCCCTTTTG AATTCTCCAC TGCCCACGTG 
CCATTTGATT TTGAAGATGA TTTTGATCTT CTCTTCAGTT CGGAGGAACC AAAGCTGACA 
TCTGCTGTAG CAGAACACAA GCGTGCCGTC CGGCCAGCCC GCCGGGTGCA AGCGTCGGGA 
AACCCACTGA AGATGCTTGC GGCCAGGGAG GACATCCTAC AGGAGTACAC AGAGACGCGG 
CTGAACGTGG CGTTCATGGA GTCCAAGAGA ATGAAAGTAG AAAAAATGTC CAAGAACTCA 
AACTTCTCAG AAGTCGCACT TGCAGGCCTG GCAAGCAAAG AAAACTTTAC CAGCGTAAGC 
CTGCGGAGTG TCAATATGAC CGAGCAGCAC TCCAACAACA GCGCCGTGCC ATACAAGAAG 
CTTATGCTAG TTCAGATTAA AGGACGCAGG CACGTTCAGT CGAGGCTGGT GGAACCGCGT 
GCATCCTCTC TGAATAGCGG AGACTGCTTC CTGCTGATCA CCCCGCACCA CTGCTTCTTG 
TGGGTCGGAG AGTTTGCAAA CGTCATTGAG AAAGCCAAGG CTTCTGAGCT GGCATCAATA 
ATTCAGACCA AGAGGGAACT TGGATGCAGG GCATCGTATG TGCAAACAGT CGAGGAAGGA 
ATAAATACCC ACACGCATGG AGCCAAGGAT TTTTGGAAAC TTCTGAGCGG TCAGACTGAA 
TCTCAAGCTG CTGGGAGCCC AGAGGAGGAT GAGAGATATG AAAATGCCAT CATAGAAACA 
AACTGTATTT ATCGATTGCT GGAAGATAAG CTGGTCCCTG TTGATGAATT TTGGGGCAAA 
GTACCTAAGT GTTCACTTTT AGAGTCTAAA GAGGTGCTTG TGTTTGATTT TGGAAGTGAA 
GTGTATGTGT GGCATGGGAA GGAGGTGACC TTGGCACAGA GGAAGGTGGC GTTCCAGTTA 
GCCAAACATC TCTGGAATGG AGCTTTTGAC TATGAAAATT GTGATATCAA TCCTCTGGAC 
CCTGGGGAAT GCAATTCTCT CATTCCAAGG AAAGGGCACG GGCGCCCTGA TTGGGCAATA 
TTTGGAAGGC TGACAGAGCA TAATGAAACC ATACTGTTCA AGGAAAAGTT CCTGGATTGG 
ACAGAAATGA AGAAACCCAA TGAGAAGGGT GTGTGCGAGG ACTCACCGAA GCTTAAGGAA 
GACCCACAAT CTGATGTGAA GCCTTATGAC ATCATGCTGA TGGTCCCAGT TCCCCAGAAA 
TCTGTGGGTA CGGTTCTTGA TGGATTAAAT GTCGCCCGTG GTTATGGACT TATTGAGGGA 
GATGATTGCC GCCAGTATGA AATCTCAACC GTCTCGGTGG ATGTCTGGCA TATCCTTGAA 
TTCGACTACA GCCGGCTGCC GAAGCAGAGC ATTGGCCAGT TCCACGAGGG AGACGCCTAT 
GTGGTAAAAT GGAAGTACTT TGTGAGCACA ACAGTTGGAC AGCGCCAGAA AACCGAGCAC 
GTCCGGATAG CGGGTAAAGA GAAGTGCGCC TACTTCTTCT GGCAGGGACG GAACTCGCCC 
GTAAGTGAGA AGGGAACCTC AGCGCTGATG ACTGTGGAGC TTGATGAAGA GCGGGGAGCA 
CAGGTCCAGG TGCTTCAGGG AAAAGAGCCC CCCTGTTTCT TACAATGTTT CCAGGGAGGA 
ATGATTGTGC ATGCTGGGAG ACGGGAAGAA GAGGAAGAGA ACACGCAAAG TGACTGGCGG 
TTGTACTGTG TACGTGGGGA GGTGCCGGTG GAAGGGAATT TGATGGAAGT CGCTTGTCAC 
TGCAGCAGCC TGCGCTCCAG GACCTCCTTG ATACTCCTCA GTATAAATAA AGCGCTCATC 
TACCTTTGGC ACGGCTGCAA AGTCCAAATC CATGCCAAGG AAGTGGGACG GACGGCAGCC 
AATAAAATAA AAGAGGAGTG CCCCCTGGAA GCCGGGCTAC ACAGCAGCAG TAAGGTGACC 
ATATATGAAT GTGATGAAGG CTCGGAACCC CCCGGATTCT GGGATGCGCT GGGCAGGCGA 
GACCGTAAAG CGTACGACTG CATGTTGCAA GATCCTGGGA AATTTAACTT CACGCCACGA 
CTGTTCAGCC TCAGTAGTTC ATCGGGAGAG TTTACAGCAT CAGAAATCCT GTATCCTTCA 
AGGCATCCGA ATGTGGTTAA CTCCATGCCA TTTTTACAAG AAGACCTGTA TACGGCCTCC 
CAGCCAGCTT TATTCCTCGT TGACAACCAT CACGAGGTTT ACCTGTGGCA AGGTTGGTGG 
CCGTCTGAGA ATATTATTAC TGGGTCTGCT CGTATTCGGT GGGATTCAGA CAGAAAAAGC 
GCAATGGAGA CCGTACTCAG CTACTGCAAA GGAAAGAATA TCAAGAAACC TCCCAAGTCG 
TATCTAATCC ATGCCGGGCT GGAGCCTCTG ACCTTTACCA ACATGTTTCC AAGCTGGGAA 
CATAGGGAAG ACATTGCCCA AATCACAGAA AAGGATGCGG AAGTCTCTAA CCAGATAATC 
CTTGTAGAAG ATGTACTCGC TAAACTTTGT AAAAGAATCT ACCCCTTGGC AGATCTTCTT 
GCCCGCCCGT TGCCCGAAGG AGTCGACCCT CTCAAGCTTG AGCTCTATCT GTCAGATAAA 
GACTTTGAGG TTGCGTTAGA AATGACAAGA GAAGAATACA ACACACTGCC GCCCTGGAAA 
CAGGTTAATA CAAAGAAAGC CAAAGGACTT TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

svil ( XM_012965657.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821111.1 Xenopus tropicalis supervillin (svil), transcript variant X2, mRNA.

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CloneID	OXa29955
Clone ID Related Accession (Same CDS sequence)	XM_012965657.2 , XM_012965657.3
Accession Version	XM_012965657.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	supervillin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965657.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCGAA ACCGCTGGAT CTCCAGCTAT GCTGGGAGTA TTATCTGTCA TGAGGATCCA 
GGATTTGATC GAGAAGGTAC ATTTCAACAC CGAACGTCAC GTCTTCCACA GAAAAAGCCG 
CCAAGTTTTC AGAAGAGTTT TGTTATTGAA GTTACTGGGG ATTTCACAAA CAACTCCAGG 
GAAACTGATG CACATCTGCT AGCTGTACTT CCAAAAGTTT CAGAACTAAG GAAACTCTTT 
GAGCCAAGCA GGAGATCCAT TTCAGAGATG AAAAGAAAAG AAAGAATAGC CAGACGTTTG 
GAAGGAATTG ATAACGACCC ACAACCAATT ATGCTACAGA CACGTTTATT GGAAGAAGAT 
ATGCCACGAT ATATGCGCGC AACAGATGCA TATAATCCAC ACATAGGTCA TGCAAATGAA 
GACACTGCCT CAGAGTCATC AATAGAAACA CATTCTAGAT GGCAAAACCG CTGGCAGCCA 
AGCACTGAAT CATCTTCTAT GTCAGGTCAA ATGGCGGTAG AGGAAACAGA ATCAAAAGCA 
GAAAGGATAG CTCGATACAA GGCGGAGCGA AGACGGCAAT TGGCAGAAAG ATATGGTGTC 
CCTATGGATT CCGAAGCGGA TTCCGAATAC CAGTCAAGGT ACACGAGAAT AAGGAAAGAC 
GGTGAGATCT CTGAAAAGAA ATCTGTGTTA GAGGAAGATG AAATTAAAGA GAATAGCTCA 
GATTATTTAT ACCGTCAAGA CATTAAGGAG CCTCGCAGAT TTCGAATGTA CGAATCAAAG 
CATCGGGACA TTAATGAGAA TGTGTCGGAG AGAGATGCTC TCAAAAATGT GGAAAACAAT 
AATAATCAAT ATCGAGAAAT ATCCGAGTTG GATGGATCTA CCTTTTCGTG CATGGGACAA 
GCCATCACAG TTGGGGAAGC ACCCAGGTCT CCAAAGTTCC CTCGTCGTGC ATCAGAGAAA 
TCACTTCAGC TTTCCTCCTC TCCACCATCT GAACAGCTAC TGCTTAGCGA CTCCAGACAA 
GGAACAGGAA AACAGGCACC AGAGTGGTCT CTCCAAAAAG ATTCCGATGG GGACACAGCG 
TCTCTCATCA GCTGGCCGTC CAGAGTTAAA GTTAGAGAGA GGCTGGGGAG AGAACATATT 
CACGGATTTC CCAAAACTGA CACAGAGATC TCAACGCAAA GGAAACATCC ACCCGGGCCT 
GCCCACCCTG TTCCTTTCCA GTCGGAAACC TCTGCTTTTC ATAGGGTTTA TAAACAGCCG 
GCATTAGTTC ACCAGCCCAT TCATGGCTAC ATCCAGCCTG CTAGTGTTGC TCATACACCT 
AAAATTGTGG CACCTCATGC ACCTGATTCG AGTGTCATGG TGTCCGCTTT TACTACCTCC 
TTAAAAAATC CCAGATCTTC CAGTGATGTG TATATTGAGA AATCCCACCA TGCATTTCAA 
GGTCAGGACA ATATCGTAGA GCCATGCAAG CAGGTGATCA GCAATGGCAA ATCGCACAGT 
ATGTATGAAG AGAAGCCAAC ATATATTTCA TCTGCCAGCA AGCCAAGAAA CGAGACCTTG 
CCTGGGCATA AAGACCCAGA CCTGCATGTT TCACACAGTT TACCACTTCA GAGACAAACC 
TCTGGAAACT TTGGGAAAAG CGAAAAGGTC TTGTTCCTTC AGAGTGGCAG ATCCCAGGAC 
CATACCAAGG AAGTATCTAC GATGAAGCAT AAAATGCTGA CCCGTTCAAT GTCTGACACC 
ACCAACTCCC AAAAGATGGA AGCTTTTAAG ATAAAGGACT CCGATGTCCA GAAAGAGATG 
GTGTTTTTGC CTTCTGAAGA CCATGTTGCC ATTCCAAATG GAGAAGCCGG CATTGTCAAT 
ACCAAAGTGT CAGTTGCTCA GCTTAGGAGT GCATATTTGG AGACGGCCAG CCACGCACAA 
AAATCTGAGT TTGAACCTAA GGCTGAAATG CCAACGTCAG GTGCTTTGTC TGCTACTACT 
GAGCGGGAAA GAGGCCCTCG AAGGCCGAGA CTTTATTTCT CTCCTGGTGA AAGTAGAAAG 
ACCTCTGAAA GATTTAGAAC CCAGCCTGTA ACGTCAGCTG AGCGAAATCA GATGGATAGA 
TCAAGCTTCC AGTCACCAGA GCAGATTGCT GAAGAAGAAG CAAAAGTGGA TGAAAGAGCC 
AAACTTAGCG TGGCTGCGAA AAGGCTTCTA TTCAGGGAAA TGGAAAAATC TCTGGAGTCG 
AAACCAGCCA AGCCACGTGC CAGAAATGCA GCAGTGGAGA GGAGGCTTCG CCGAGCACAA 
GACAGGTCTC GGACGCAACC CGTTACCAGT GAAGAGGTGG TCATTGCTGC TACTGAGCCT 
AGTGCTGCTT CGCACACTGT GACCACACAC ACGGCCCTCC CTCGAATACC TAGCCCCACT 
GTAACTAAGA GCTCTGTACA CCATATCAGT TTGCAGGCAT CATCCCATAA AAGCACAATA 
TCAAAGCAAG AGGAGATACA AAAGGAAATA CACGATGACC AGAGCATTCC CGAAGAGCCT 
GATAATTCCT CGCTTTCCTT GGCTGAAAAA ATGGCATTAT TTAATAAGCT TTCCCAGCCA 
ATAACAAAAG AGGTGTCTAC GAGGAGCAGA ATGGATATTC GACAAAGGCG AATGAATGCG 
CGTTACCAGA CTCAGCCCGT CACCCTTGTG GAGGTTGAAC AGTATAAGAA TGGAGGCGAA 
AAGATCGGCC CCTTATCTCA CAGTGTTGTA ACTTCCGTAG CCACCCTAAC CTCTCGGCCG 
TCTCCAGTTA GCCCTGGGGA TATGCATACT GCAAAGTCCT CCAGTTATGA AGAGAGAATA 
TCAAGTGTCT CCAGCACTTC AGCCCTGCTG CACACTTCAC TGGAAACAGG AAGCACTTCG 
GAAAGGAGCT TGCTGCTCTC GGAGCCCTGG AGTCCCCCAA GTGTGAAAGA TAAAGTATAT 
GAAAAGGAAC CGAGGAAACC AATGTTGGAA GAGACAAGGG TCTCTGCAGC AGAAGATAGC 
AGCAGCTTCG GAAAATGCAG CTCGCTCCAT AAGGAAAAAA CCCACCCCGC GCTTGATGTG 
GCAAAGGAGG AAGAACATGT TGAGGAGCAG AAAGTGCCTG GGCATGTTGC CTCAAGTAGC 
AGCAGAGTGG AGTTGCTCAG CCCCCAGAAG CTGTCCCAGC CTGCTGAGCA TTCAGAATCC 
CAAGCAAAGC CTGAAAGTGC CCTGCAGGTA GACGCTGAAG ATAAGAAGAG CCTACATCCC 
GAGCCCAGCA TGAGAAGAAG GAAAGAGATC CACGAGATTG TTGAGCCGGC CTCTGAACTC 
CTTGGCACCA GCGCTGCAAA GACCGTTTCT CACACTCCAG CTGCTGCTCC TGCTGCGGTG 
TCCTACGGAC CACAGGCATC AGAGCTAAGT GAGGAAAAGC CCGAGGAGGA AACAGCTGAG 
ATCTCCTCCA CCATCAGCAA CAGGTCCAAC GAGGATCTCG ATACGCCCGG CAAAGCCATG 
TCCATCAAAG ACAGGCTGGC GCTGCTAAAG AAGAGTGGGG AAGAGGATTG GAAAAACAGG 
CTTAACAAAA AGCAGGAGTA CAGCAAAGTG TCTGTCATCG AGCGCAGCAC GCTCACCCAG 
CTCCATGAGA TTGAGCAGTC GCTGACAAAG AAGGAACCTG TCTATGCTTC TGTTTATTCT 
CCTGCTCTAC CTGCCGCCCA TAAATGCCAG TATTTTGTTC CTATACATCA GGTCAGTAAT 
GCTACGACCC TGGGGCAGTC CAGCCCTGTG ATGTTCGATG AGCGGCACCC CTGGAGATGG 
AAGCAAAGGG TGACTGACGG CAATGGGCTT CACGTGTCTA TTGAAGAAAG GAAGCAGCAG 
ATTACAGTCA GGGAGGAGGC CTGGAAAACC AAAGGCATAG GGGCAGCAAA TGATTCCACA 
CAATTTACAG TGGCCGGCCG CATGGTTAAG AAAGGTCTGG CCTCACCTAC AGCCATTACC 
CCCGTCGCAT CTCCAATGTC TCTCAAATTT AAAACTCCAG TTTCCAAGCC TCTGGAAGAA 
ATTGAAGCCA AACCTGATAT GAATTTGGAA TCTGACAGGA AGTTAGAAAA ACTAGAATCA 
TTTTTGGGAA AACTGCACAA CAGGGTTGGA GGGCTACAAG ATACCACACT GTCAGTCACA 
GGCCAATCAG TGAAGGAGGT GATGAAGGTG GACGATGATG AAACTTTCGG AAGGTTCTAC 
CGCCCTTTTG AATTCTCCAC TGCCCACGTG CCATTTGATT TTGAAGATGA TTTTGATCTT 
CTCTTCAGTT CGGAGGAACC AAAGCTGACA TCTGCTGTAG CAGAACACAA GCGTGCCGTC 
CGGCCAGCCC GCCGGGTGCA AGCGTCGGGA AACCCACTGA AGATGCTTGC GGCCAGGGAG 
GACATCCTAC AGGAGTACAC AGAGACGCGG CTGAACGTGG CGTTCATGGA GTCCAAGAGA 
ATGAAAGTAG AAAAAATGTC CAAGAACTCA AACTTCTCAG AAGTCGCACT TGCAGGCCTG 
GCAAGCAAAG AAAACTTTAC CAGCGTAAGC CTGCGGAGTG TCAATATGAC CGAGCAGCAC 
TCCAACAACA GCGCCGTGCC ATACAAGAAG CTTATGCTAG TTCAGATTAA AGGACGCAGG 
CACGTTCAGT CGAGGCTGGT GGAACCGCGT GCATCCTCTC TGAATAGCGG AGACTGCTTC 
CTGCTGATCA CCCCGCACCA CTGCTTCTTG TGGGTCGGAG AGTTTGCAAA CGTCATTGAG 
AAAGCCAAGG CTTCTGAGCT GGCATCAATA ATTCAGACCA AGAGGGAACT TGGATGCAGG 
GCATCGTATG TGCAAACAGT CGAGGAAGGA ATAAATACCC ACACGCATGG AGCCAAGGAT 
TTTTGGAAAC TTCTGAGCGG TCAGACTGAA TCTCAAGCTG CTGGGAGCCC AGAGGAGGAT 
GAGAGATATG AAAATGCCAT CATAGAAACA AACTGTATTT ATCGATTGCT GGAAGATAAG 
CTGGTCCCTG TTGATGAATT TTGGGGCAAA GTACCTAAGT GTTCACTTTT AGAGTCTAAA 
GAGGTGCTTG TGTTTGATTT TGGAAGTGAA GTGTATGTGT GGCATGGGAA GGAGGTGACC 
TTGGCACAGA GGAAGGTGGC GTTCCAGTTA GCCAAACATC TCTGGAATGG AGCTTTTGAC 
TATGAAAATT GTGATATCAA TCCTCTGGAC CCTGGGGAAT GCAATTCTCT CATTCCAAGG 
AAAGGGCACG GGCGCCCTGA TTGGGCAATA TTTGGAAGGC TGACAGAGCA TAATGAAACC 
ATACTGTTCA AGGAAAAGTT CCTGGATTGG ACAGAAATGA AGAAACCCAA TGAGAAGGGT 
GTGTGCGAGG ACTCACCGAA GCTTAAGGAA GACCCACAAT CTGATGTGAA GCCTTATGAC 
ATCATGCTGA TGGTCCCAGT TCCCCAGAAA TCTGTGGGTA CGGTTCTTGA TGGATTAAAT 
GTCGCCCGTG GTTATGGACT TATTGAGGGA GATGATTGCC GCCAGTATGA AATCTCAACC 
GTCTCGGTGG ATGTCTGGCA TATCCTTGAA TTCGACTACA GCCGGCTGCC GAAGCAGAGC 
ATTGGCCAGT TCCACGAGGG AGACGCCTAT GTGGTAAAAT GGAAGTACTT TGTGAGCACA 
ACAGTTGGAC AGCGCCAGAA AACCGAGCAC GTCCGGATAG CGGGTAAAGA GAAGTGCGCC 
TACTTCTTCT GGCAGGGACG GAACTCGCCC GTAAGTGAGA AGGGAACCTC AGCGCTGATG 
ACTGTGGAGC TTGATGAAGA GCGGGGAGCA CAGGTCCAGG TGCTTCAGGG AAAAGAGCCC 
CCCTGTTTCT TACAATGTTT CCAGGGAGGA ATGATTGTGC ATGCTGGGAG ACGGGAAGAA 
GAGGAAGAGA ACACGCAAAG TGACTGGCGG TTGTACTGTG TACGTGGGGA GGTGCCGGTG 
GAAGGGAATT TGATGGAAGT CGCTTGTCAC TGCAGCAGCC TGCGCTCCAG GACCTCCTTG 
ATACTCCTCA GTATAAATAA AGCGCTCATC TACCTTTGGC ACGGCTGCAA AGTCCAAATC 
CATGCCAAGG AAGTGGGACG GACGGCAGCC AATAAAATAA AAGAGGAGTG CCCCCTGGAA 
GCCGGGCTAC ACAGCAGCAG TAAGGTGACC ATATATGAAT GTGATGAAGG CTCGGAACCC 
CCCGGATTCT GGGATGCGCT GGGCAGGCGA GACCGTAAAG CGTACGACTG CATGTTGCAA 
GATCCTGGGA AATTTAACTT CACGCCACGA CTGTTCAGCC TCAGTAGTTC ATCGGGAGAG 
TTTACAGCAT CAGAAATCCT GTATCCTTCA AGGCATCCGA ATGTGGTTAA CTCCATGCCA 
TTTTTACAAG AAGACCTGTA TACGGCCTCC CAGCCAGCTT TATTCCTCGT TGACAACCAT 
CACGAGGTTT ACCTGTGGCA AGGTTGGTGG CCGTCTGAGA ATATTATTAC TGGGTCTGCT 
CGTATTCGGT GGGATTCAGA CAGAAAAAGC GCAATGGAGA CCGTACTCAG CTACTGCAAA 
GGAAAGAATA TCAAGAAACC TCCCAAGTCG TATCTAATCC ATGCCGGGCT GGAGCCTCTG 
ACCTTTACCA ACATGTTTCC AAGCTGGGAA CATAGGGAAG ACATTGCCCA AATCACAGAA 
AAGGATGCGG AAGTCTCTAA CCAGATAATC CTTGTAGAAG ATGTACTCGC TAAACTTTGT 
AAAAGAATCT ACCCCTTGGC AGATCTTCTT GCCCGCCCGT TGCCCGAAGG AGTCGACCCT 
CTCAAGCTTG AGCTCTATCT GTCAGATAAA GACTTTGAGG TTGCGTTAGA AATGACAAGA 
GAAGAATACA ACACACTGCC GCCCTGGAAA CAGGTTAATA CAAAGAAAGC CAAAGGACTT 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821111.1
CDS	68..6973
Translation	MFRNRWISSYAGSIICHEDPGFDREGTFQHRTSRLPQKKPPSFQKSFVIEVTGDFTNNSRETDAHLLAVLPKVSELRKLFEPSRRSISEMKRKERIARRLEGIDNDPQPIMLQTRLLEEDMPRYMRATDAYNPHIGHANEDTASESSIETHSRWQNRWQPSTESSSMSGQMAVEETESKAERIARYKAERRRQLAERYGVPMDSEADSEYQSRYTRIRKDGEISEKKSVLEEDEIKENSSDYLYRQDIKEPRRFRMYESKHRDINENVSERDALKNVENNNNQYREISELDGSTFSCMGQAITVGEAPRSPKFPRRASEKSLQLSSSPPSEQLLLSDSRQGTGKQAPEWSLQKDSDGDTASLISWPSRVKVRERLGREHIHGFPKTDTEISTQRKHPPGPAHPVPFQSETSAFHRVYKQPALVHQPIHGYIQPASVAHTPKIVAPHAPDSSVMVSAFTTSLKNPRSSSDVYIEKSHHAFQGQDNIVEPCKQVISNGKSHSMYEEKPTYISSASKPRNETLPGHKDPDLHVSHSLPLQRQTSGNFGKSEKVLFLQSGRSQDHTKEVSTMKHKMLTRSMSDTTNSQKMEAFKIKDSDVQKEMVFLPSEDHVAIPNGEAGIVNTKVSVAQLRSAYLETASHAQKSEFEPKAEMPTSGALSATTERERGPRRPRLYFSPGESRKTSERFRTQPVTSAERNQMDRSSFQSPEQIAEEEAKVDERAKLSVAAKRLLFREMEKSLESKPAKPRARNAAVERRLRRAQDRSRTQPVTSEEVVIAATEPSAASHTVTTHTALPRIPSPTVTKSSVHHISLQASSHKSTISKQEEIQKEIHDDQSIPEEPDNSSLSLAEKMALFNKLSQPITKEVSTRSRMDIRQRRMNARYQTQPVTLVEVEQYKNGGEKIGPLSHSVVTSVATLTSRPSPVSPGDMHTAKSSSYEERISSVSSTSALLHTSLETGSTSERSLLLSEPWSPPSVKDKVYEKEPRKPMLEETRVSAAEDSSSFGKCSSLHKEKTHPALDVAKEEEHVEEQKVPGHVASSSSRVELLSPQKLSQPAEHSESQAKPESALQVDAEDKKSLHPEPSMRRRKEIHEIVEPASELLGTSAAKTVSHTPAAAPAAVSYGPQASELSEEKPEEETAEISSTISNRSNEDLDTPGKAMSIKDRLALLKKSGEEDWKNRLNKKQEYSKVSVIERSTLTQLHEIEQSLTKKEPVYASVYSPALPAAHKCQYFVPIHQVSNATTLGQSSPVMFDERHPWRWKQRVTDGNGLHVSIEERKQQITVREEAWKTKGIGAANDSTQFTVAGRMVKKGLASPTAITPVASPMSLKFKTPVSKPLEEIEAKPDMNLESDRKLEKLESFLGKLHNRVGGLQDTTLSVTGQSVKEVMKVDDDETFGRFYRPFEFSTAHVPFDFEDDFDLLFSSEEPKLTSAVAEHKRAVRPARRVQASGNPLKMLAAREDILQEYTETRLNVAFMESKRMKVEKMSKNSNFSEVALAGLASKENFTSVSLRSVNMTEQHSNNSAVPYKKLMLVQIKGRRHVQSRLVEPRASSLNSGDCFLLITPHHCFLWVGEFANVIEKAKASELASIIQTKRELGCRASYVQTVEEGINTHTHGAKDFWKLLSGQTESQAAGSPEEDERYENAIIETNCIYRLLEDKLVPVDEFWGKVPKCSLLESKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGAFDYENCDINPLDPGECNSLIPRKGHGRPDWAIFGRLTEHNETILFKEKFLDWTEMKKPNEKGVCEDSPKLKEDPQSDVKPYDIMLMVPVPQKSVGTVLDGLNVARGYGLIEGDDCRQYEISTVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKYFVSTTVGQRQKTEHVRIAGKEKCAYFFWQGRNSPVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMIVHAGRREEEEENTQSDWRLYCVRGEVPVEGNLMEVACHCSSLRSRTSLILLSINKALIYLWHGCKVQIHAKEVGRTAANKIKEECPLEAGLHSSSKVTIYECDEGSEPPGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFTASEILYPSRHPNVVNSMPFLQEDLYTASQPALFLVDNHHEVYLWQGWWPSENIITGSARIRWDSDRKSAMETVLSYCKGKNIKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAQITEKDAEVSNQIILVEDVLAKLCKRIYPLADLLARPLPEGVDPLKLELYLSDKDFEVALEMTREEYNTLPPWKQVNTKKAKGLF

Target ORF information:

RefSeq Version	XM_012965657.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis supervillin (svil), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965657.3

ORF Insert Sequence:

ATGTTCCGAA ACCGCTGGAT CTCCAGCTAT GCTGGGAGTA TTATCTGTCA TGAGGATCCA 
GGATTTGATC GAGAAGGTAC ATTTCAACAC CGAACGTCAC GTCTTCCACA GAAAAAGCCG 
CCAAGTTTTC AGAAGAGTTT TGTTATTGAA GTTACTGGGG ATTTCACAAA CAACTCCAGG 
GAAACTGATG CACATCTGCT AGCTGTACTT CCAAAAGTTT CAGAACTAAG GAAACTCTTT 
GAGCCAAGCA GGAGATCCAT TTCAGAGATG AAAAGAAAAG AAAGAATAGC CAGACGTTTG 
GAAGGAATTG ATAACGACCC ACAACCAATT ATGCTACAGA CACGTTTATT GGAAGAAGAT 
ATGCCACGAT ATATGCGCGC AACAGATGCA TATAATCCAC ACATAGGTCA TGCAAATGAA 
GACACTGCCT CAGAGTCATC AATAGAAACA CATTCTAGAT GGCAAAACCG CTGGCAGCCA 
AGCACTGAAT CATCTTCTAT GTCAGGTCAA ATGGCGGTAG AGGAAACAGA ATCAAAAGCA 
GAAAGGATAG CTCGATACAA GGCGGAGCGA AGACGGCAAT TGGCAGAAAG ATATGGTGTC 
CCTATGGATT CCGAAGCGGA TTCCGAATAC CAGTCAAGGT ACACGAGAAT AAGGAAAGAC 
GGTGAGATCT CTGAAAAGAA ATCTGTGTTA GAGGAAGATG AAATTAAAGA GAATAGCTCA 
GATTATTTAT ACCGTCAAGA CATTAAGGAG CCTCGCAGAT TTCGAATGTA CGAATCAAAG 
CATCGGGACA TTAATGAGAA TGTGTCGGAG AGAGATGCTC TCAAAAATGT GGAAAACAAT 
AATAATCAAT ATCGAGAAAT ATCCGAGTTG GATGGATCTA CCTTTTCGTG CATGGGACAA 
GCCATCACAG TTGGGGAAGC ACCCAGGTCT CCAAAGTTCC CTCGTCGTGC ATCAGAGAAA 
TCACTTCAGC TTTCCTCCTC TCCACCATCT GAACAGCTAC TGCTTAGCGA CTCCAGACAA 
GGAACAGGAA AACAGGCACC AGAGTGGTCT CTCCAAAAAG ATTCCGATGG GGACACAGCG 
TCTCTCATCA GCTGGCCGTC CAGAGTTAAA GTTAGAGAGA GGCTGGGGAG AGAACATATT 
CACGGATTTC CCAAAACTGA CACAGAGATC TCAACGCAAA GGAAACATCC ACCCGGGCCT 
GCCCACCCTG TTCCTTTCCA GTCGGAAACC TCTGCTTTTC ATAGGGTTTA TAAACAGCCG 
GCATTAGTTC ACCAGCCCAT TCATGGCTAC ATCCAGCCTG CTAGTGTTGC TCATACACCT 
AAAATTGTGG CACCTCATGC ACCTGATTCG AGTGTCATGG TGTCCGCTTT TACTACCTCC 
TTAAAAAATC CCAGATCTTC CAGTGATGTG TATATTGAGA AATCCCACCA TGCATTTCAA 
GGTCAGGACA ATATCGTAGA GCCATGCAAG CAGGTGATCA GCAATGGCAA ATCGCACAGT 
ATGTATGAAG AGAAGCCAAC ATATATTTCA TCTGCCAGCA AGCCAAGAAA CGAGACCTTG 
CCTGGGCATA AAGACCCAGA CCTGCATGTT TCACACAGTT TACCACTTCA GAGACAAACC 
TCTGGAAACT TTGGGAAAAG CGAAAAGGTC TTGTTCCTTC AGAGTGGCAG ATCCCAGGAC 
CATACCAAGG AAGTATCTAC GATGAAGCAT AAAATGCTGA CCCGTTCAAT GTCTGACACC 
ACCAACTCCC AAAAGATGGA AGCTTTTAAG ATAAAGGACT CCGATGTCCA GAAAGAGATG 
GTGTTTTTGC CTTCTGAAGA CCATGTTGCC ATTCCAAATG GAGAAGCCGG CATTGTCAAT 
ACCAAAGTGT CAGTTGCTCA GCTTAGGAGT GCATATTTGG AGACGGCCAG CCACGCACAA 
AAATCTGAGT TTGAACCTAA GGCTGAAATG CCAACGTCAG GTGCTTTGTC TGCTACTACT 
GAGCGGGAAA GAGGCCCTCG AAGGCCGAGA CTTTATTTCT CTCCTGGTGA AAGTAGAAAG 
ACCTCTGAAA GATTTAGAAC CCAGCCTGTA ACGTCAGCTG AGCGAAATCA GATGGATAGA 
TCAAGCTTCC AGTCACCAGA GCAGATTGCT GAAGAAGAAG CAAAAGTGGA TGAAAGAGCC 
AAACTTAGCG TGGCTGCGAA AAGGCTTCTA TTCAGGGAAA TGGAAAAATC TCTGGAGTCG 
AAACCAGCCA AGCCACGTGC CAGAAATGCA GCAGTGGAGA GGAGGCTTCG CCGAGCACAA 
GACAGGTCTC GGACGCAACC CGTTACCAGT GAAGAGGTGG TCATTGCTGC TACTGAGCCT 
AGTGCTGCTT CGCACACTGT GACCACACAC ACGGCCCTCC CTCGAATACC TAGCCCCACT 
GTAACTAAGA GCTCTGTACA CCATATCAGT TTGCAGGCAT CATCCCATAA AAGCACAATA 
TCAAAGCAAG AGGAGATACA AAAGGAAATA CACGATGACC AGAGCATTCC CGAAGAGCCT 
GATAATTCCT CGCTTTCCTT GGCTGAAAAA ATGGCATTAT TTAATAAGCT TTCCCAGCCA 
ATAACAAAAG AGGTGTCTAC GAGGAGCAGA ATGGATATTC GACAAAGGCG AATGAATGCG 
CGTTACCAGA CTCAGCCCGT CACCCTTGTG GAGGTTGAAC AGTATAAGAA TGGAGGCGAA 
AAGATCGGCC CCTTATCTCA CAGTGTTGTA ACTTCCGTAG CCACCCTAAC CTCTCGGCCG 
TCTCCAGTTA GCCCTGGGGA TATGCATACT GCAAAGTCCT CCAGTTATGA AGAGAGAATA 
TCAAGTGTCT CCAGCACTTC AGCCCTGCTG CACACTTCAC TGGAAACAGG AAGCACTTCG 
GAAAGGAGCT TGCTGCTCTC GGAGCCCTGG AGTCCCCCAA GTGTGAAAGA TAAAGTATAT 
GAAAAGGAAC CGAGGAAACC AATGTTGGAA GAGACAAGGG TCTCTGCAGC AGAAGATAGC 
AGCAGCTTCG GAAAATGCAG CTCGCTCCAT AAGGAAAAAA CCCACCCCGC GCTTGATGTG 
GCAAAGGAGG AAGAACATGT TGAGGAGCAG AAAGTGCCTG GGCATGTTGC CTCAAGTAGC 
AGCAGAGTGG AGTTGCTCAG CCCCCAGAAG CTGTCCCAGC CTGCTGAGCA TTCAGAATCC 
CAAGCAAAGC CTGAAAGTGC CCTGCAGGTA GACGCTGAAG ATAAGAAGAG CCTACATCCC 
GAGCCCAGCA TGAGAAGAAG GAAAGAGATC CACGAGATTG TTGAGCCGGC CTCTGAACTC 
CTTGGCACCA GCGCTGCAAA GACCGTTTCT CACACTCCAG CTGCTGCTCC TGCTGCGGTG 
TCCTACGGAC CACAGGCATC AGAGCTAAGT GAGGAAAAGC CCGAGGAGGA AACAGCTGAG 
ATCTCCTCCA CCATCAGCAA CAGGTCCAAC GAGGATCTCG ATACGCCCGG CAAAGCCATG 
TCCATCAAAG ACAGGCTGGC GCTGCTAAAG AAGAGTGGGG AAGAGGATTG GAAAAACAGG 
CTTAACAAAA AGCAGGAGTA CAGCAAAGTG TCTGTCATCG AGCGCAGCAC GCTCACCCAG 
CTCCATGAGA TTGAGCAGTC GCTGACAAAG AAGGAACCTG TCTATGCTTC TGTTTATTCT 
CCTGCTCTAC CTGCCGCCCA TAAATGCCAG TATTTTGTTC CTATACATCA GGTCAGTAAT 
GCTACGACCC TGGGGCAGTC CAGCCCTGTG ATGTTCGATG AGCGGCACCC CTGGAGATGG 
AAGCAAAGGG TGACTGACGG CAATGGGCTT CACGTGTCTA TTGAAGAAAG GAAGCAGCAG 
ATTACAGTCA GGGAGGAGGC CTGGAAAACC AAAGGCATAG GGGCAGCAAA TGATTCCACA 
CAATTTACAG TGGCCGGCCG CATGGTTAAG AAAGGTCTGG CCTCACCTAC AGCCATTACC 
CCCGTCGCAT CTCCAATGTC TCTCAAATTT AAAACTCCAG TTTCCAAGCC TCTGGAAGAA 
ATTGAAGCCA AACCTGATAT GAATTTGGAA TCTGACAGGA AGTTAGAAAA ACTAGAATCA 
TTTTTGGGAA AACTGCACAA CAGGGTTGGA GGGCTACAAG ATACCACACT GTCAGTCACA 
GGCCAATCAG TGAAGGAGGT GATGAAGGTG GACGATGATG AAACTTTCGG AAGGTTCTAC 
CGCCCTTTTG AATTCTCCAC TGCCCACGTG CCATTTGATT TTGAAGATGA TTTTGATCTT 
CTCTTCAGTT CGGAGGAACC AAAGCTGACA TCTGCTGTAG CAGAACACAA GCGTGCCGTC 
CGGCCAGCCC GCCGGGTGCA AGCGTCGGGA AACCCACTGA AGATGCTTGC GGCCAGGGAG 
GACATCCTAC AGGAGTACAC AGAGACGCGG CTGAACGTGG CGTTCATGGA GTCCAAGAGA 
ATGAAAGTAG AAAAAATGTC CAAGAACTCA AACTTCTCAG AAGTCGCACT TGCAGGCCTG 
GCAAGCAAAG AAAACTTTAC CAGCGTAAGC CTGCGGAGTG TCAATATGAC CGAGCAGCAC 
TCCAACAACA GCGCCGTGCC ATACAAGAAG CTTATGCTAG TTCAGATTAA AGGACGCAGG 
CACGTTCAGT CGAGGCTGGT GGAACCGCGT GCATCCTCTC TGAATAGCGG AGACTGCTTC 
CTGCTGATCA CCCCGCACCA CTGCTTCTTG TGGGTCGGAG AGTTTGCAAA CGTCATTGAG 
AAAGCCAAGG CTTCTGAGCT GGCATCAATA ATTCAGACCA AGAGGGAACT TGGATGCAGG 
GCATCGTATG TGCAAACAGT CGAGGAAGGA ATAAATACCC ACACGCATGG AGCCAAGGAT 
TTTTGGAAAC TTCTGAGCGG TCAGACTGAA TCTCAAGCTG CTGGGAGCCC AGAGGAGGAT 
GAGAGATATG AAAATGCCAT CATAGAAACA AACTGTATTT ATCGATTGCT GGAAGATAAG 
CTGGTCCCTG TTGATGAATT TTGGGGCAAA GTACCTAAGT GTTCACTTTT AGAGTCTAAA 
GAGGTGCTTG TGTTTGATTT TGGAAGTGAA GTGTATGTGT GGCATGGGAA GGAGGTGACC 
TTGGCACAGA GGAAGGTGGC GTTCCAGTTA GCCAAACATC TCTGGAATGG AGCTTTTGAC 
TATGAAAATT GTGATATCAA TCCTCTGGAC CCTGGGGAAT GCAATTCTCT CATTCCAAGG 
AAAGGGCACG GGCGCCCTGA TTGGGCAATA TTTGGAAGGC TGACAGAGCA TAATGAAACC 
ATACTGTTCA AGGAAAAGTT CCTGGATTGG ACAGAAATGA AGAAACCCAA TGAGAAGGGT 
GTGTGCGAGG ACTCACCGAA GCTTAAGGAA GACCCACAAT CTGATGTGAA GCCTTATGAC 
ATCATGCTGA TGGTCCCAGT TCCCCAGAAA TCTGTGGGTA CGGTTCTTGA TGGATTAAAT 
GTCGCCCGTG GTTATGGACT TATTGAGGGA GATGATTGCC GCCAGTATGA AATCTCAACC 
GTCTCGGTGG ATGTCTGGCA TATCCTTGAA TTCGACTACA GCCGGCTGCC GAAGCAGAGC 
ATTGGCCAGT TCCACGAGGG AGACGCCTAT GTGGTAAAAT GGAAGTACTT TGTGAGCACA 
ACAGTTGGAC AGCGCCAGAA AACCGAGCAC GTCCGGATAG CGGGTAAAGA GAAGTGCGCC 
TACTTCTTCT GGCAGGGACG GAACTCGCCC GTAAGTGAGA AGGGAACCTC AGCGCTGATG 
ACTGTGGAGC TTGATGAAGA GCGGGGAGCA CAGGTCCAGG TGCTTCAGGG AAAAGAGCCC 
CCCTGTTTCT TACAATGTTT CCAGGGAGGA ATGATTGTGC ATGCTGGGAG ACGGGAAGAA 
GAGGAAGAGA ACACGCAAAG TGACTGGCGG TTGTACTGTG TACGTGGGGA GGTGCCGGTG 
GAAGGGAATT TGATGGAAGT CGCTTGTCAC TGCAGCAGCC TGCGCTCCAG GACCTCCTTG 
ATACTCCTCA GTATAAATAA AGCGCTCATC TACCTTTGGC ACGGCTGCAA AGTCCAAATC 
CATGCCAAGG AAGTGGGACG GACGGCAGCC AATAAAATAA AAGAGGAGTG CCCCCTGGAA 
GCCGGGCTAC ACAGCAGCAG TAAGGTGACC ATATATGAAT GTGATGAAGG CTCGGAACCC 
CCCGGATTCT GGGATGCGCT GGGCAGGCGA GACCGTAAAG CGTACGACTG CATGTTGCAA 
GATCCTGGGA AATTTAACTT CACGCCACGA CTGTTCAGCC TCAGTAGTTC ATCGGGAGAG 
TTTACAGCAT CAGAAATCCT GTATCCTTCA AGGCATCCGA ATGTGGTTAA CTCCATGCCA 
TTTTTACAAG AAGACCTGTA TACGGCCTCC CAGCCAGCTT TATTCCTCGT TGACAACCAT 
CACGAGGTTT ACCTGTGGCA AGGTTGGTGG CCGTCTGAGA ATATTATTAC TGGGTCTGCT 
CGTATTCGGT GGGATTCAGA CAGAAAAAGC GCAATGGAGA CCGTACTCAG CTACTGCAAA 
GGAAAGAATA TCAAGAAACC TCCCAAGTCG TATCTAATCC ATGCCGGGCT GGAGCCTCTG 
ACCTTTACCA ACATGTTTCC AAGCTGGGAA CATAGGGAAG ACATTGCCCA AATCACAGAA 
AAGGATGCGG AAGTCTCTAA CCAGATAATC CTTGTAGAAG ATGTACTCGC TAAACTTTGT 
AAAAGAATCT ACCCCTTGGC AGATCTTCTT GCCCGCCCGT TGCCCGAAGG AGTCGACCCT 
CTCAAGCTTG AGCTCTATCT GTCAGATAAA GACTTTGAGG TTGCGTTAGA AATGACAAGA 
GAAGAATACA ACACACTGCC GCCCTGGAAA CAGGTTAATA CAAAGAAAGC CAAAGGACTT 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

svil ( XM_012965654.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821108.1 Xenopus tropicalis supervillin (svil), transcript variant X1, mRNA.

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CloneID	OXa29953
Clone ID Related Accession (Same CDS sequence)	XM_012965654.2
Accession Version	XM_012965654.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	supervillin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965654.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGGG AAGCACAGTG CGTGTGCCCG GGTACGTGTC GGGGGCGCGC AGGCTGGTCT 
TGTGTTTTAC TGAAAAAGTC CCGTTTGGAT TTCTGCCTTA GAACTGACGT CACTTCAGAA 
GCAGGATTTG ATCGAGAAGG TACATTTCAA CACCGAACGT CACGTCTTCC ACAGAAAAAG 
CCGCCAAGTT TTCAGAAGAG TTTTGTTATT GAAGTTACTG GGGATTTCAC AAACAACTCC 
AGGGAAACTG ATGCACATCT GCTAGCTGTA CTTCCAAAAG TTTCAGAACT AAGGAAACTC 
TTTGAGCCAA GCAGGAGATC CATTTCAGAG ATGAAAAGAA AAGAAAGAAT AGCCAGACGT 
TTGGAAGGAA TTGATAACGA CCCACAACCA ATTATGCTAC AGACACGTTT ATTGGAAGAA 
GATATGCCAC GATATATGCG CGCAACAGAT GCATATAATC CACACATAGG TCATGCAAAT 
GAAGACACTG CCTCAGAGTC ATCAATAGAA ACACATTCTA GATGGCAAAA CCGCTGGCAG 
CCAAGCACTG AATCATCTTC TATGTCAGGT CAAATGGCGG TAGAGGAAAC AGAATCAAAA 
GCAGAAAGGA TAGCTCGATA CAAGGCGGAG CGAAGACGGC AATTGGCAGA AAGATATGGT 
GTCCCTATGG ATTCCGAAGC GGATTCCGAA TACCAGTCAA GGTACACGAG AATAAGGAAA 
GACGGTGAGA TCTCTGAAAA GAAATCTGTG TTAGAGGAAG ATGAAATTAA AGAGAATAGC 
TCAGATTATT TATACCGTCA AGACATTAAG GAGCCTCGCA GATTTCGAAT GTACGAATCA 
AAGCATCGGG ACATTAATGA GAATGTGTCG GAGAGAGATG CTCTCAAAAA TGTGGAAAAC 
AATAATAATC AATATCGAGA AATATCCGAG TTGGATGGAT CTACCTTTTC GTGCATGGGA 
CAAGCCATCA CAGTTGGGGA AGCACCCAGG TCTCCAAAGT TCCCTCGTCG TGCATCAGAG 
AAATCACTTC AGCTTTCCTC CTCTCCACCA TCTGAACAGC TACTGCTTAG CGACTCCAGA 
CAAGGAACAG GAAAACAGGC ACCAGAGTGG TCTCTCCAAA AAGATTCCGA TGGGGACACA 
GCGTCTCTCA TCAGCTGGCC GTCCAGAGTT AAAGTTAGAG AGAGGCTGGG GAGAGAACAT 
ATTCACGGAT TTCCCAAAAC TGACACAGAG ATCTCAACGC AAAGGAAACA TCCACCCGGG 
CCTGCCCACC CTGTTCCTTT CCAGTCGGAA ACCTCTGCTT TTCATAGGGT TTATAAACAG 
CCGGCATTAG TTCACCAGCC CATTCATGGC TACATCCAGC CTGCTAGTGT TGCTCATACA 
CCTAAAATTG TGGCACCTCA TGCACCTGAT TCGAGTGTCA TGGTGTCCGC TTTTACTACC 
TCCTTAAAAA ATCCCAGATC TTCCAGTGAT GTGTATATTG AGAAATCCCA CCATGCATTT 
CAAGGTCAGG ACAATATCGT AGAGCCATGC AAGCAGGTGA TCAGCAATGG CAAATCGCAC 
AGTATGTATG AAGAGAAGCC AACATATATT TCATCTGCCA GCAAGCCAAG AAACGAGACC 
TTGCCTGGGC ATAAAGACCC AGACCTGCAT GTTTCACACA GTTTACCACT TCAGAGACAA 
ACCTCTGGAA ACTTTGGGAA AAGCGAAAAG GTCTTGTTCC TTCAGAGTGG CAGATCCCAG 
GACCATACCA AGGAAGTATC TACGATGAAG CATAAAATGC TGACCCGTTC AATGTCTGAC 
ACCACCAACT CCCAAAAGAT GGAAGCTTTT AAGATAAAGG ACTCCGATGT CCAGAAAGAG 
ATGGTGTTTT TGCCTTCTGA AGACCATGTT GCCATTCCAA ATGGAGAAGC CGGCATTGTC 
AATACCAAAG TGTCAGTTGC TCAGCTTAGG AGTGCATATT TGGAGACGGC CAGCCACGCA 
CAAAAATCTG AGTTTGAACC TAAGGCTGAA ATGCCAACGT CAGGTGCTTT GTCTGCTACT 
ACTGAGCGGG AAAGAGGCCC TCGAAGGCCG AGACTTTATT TCTCTCCTGG TGAAAGTAGA 
AAGACCTCTG AAAGATTTAG AACCCAGCCT GTAACGTCAG CTGAGCGAAA TCAGATGGAT 
AGATCAAGCT TCCAGTCACC AGAGCAGATT GCTGAAGAAG AAGCAAAAGT GGATGAAAGA 
GCCAAACTTA GCGTGGCTGC GAAAAGGCTT CTATTCAGGG AAATGGAAAA ATCTCTGGAG 
TCGAAACCAG CCAAGCCACG TGCCAGAAAT GCAGCAGTGG AGAGGAGGCT TCGCCGAGCA 
CAAGACAGGT CTCGGACGCA ACCCGTTACC AGTGAAGAGG TGGTCATTGC TGCTACTGAG 
CCTAGTGCTG CTTCGCACAC TGTGACCACA CACACGGCCC TCCCTCGAAT ACCTAGCCCC 
ACTGTAACTA AGAGCTCTGT ACACCATATC AGTTTGCAGG CATCATCCCA TAAAAGCACA 
ATATCAAAGC AAGAGGAGAT ACAAAAGGAA ATACACGATG ACCAGAGCAT TCCCGAAGAG 
CCTGATAATT CCTCGCTTTC CTTGGCTGAA AAAATGGCAT TATTTAATAA GCTTTCCCAG 
CCAATAACAA AAGAGGTGTC TACGAGGAGC AGAATGGATA TTCGACAAAG GCGAATGAAT 
GCGCGTTACC AGACTCAGCC CGTCACCCTT GTGGAGGTTG AACAGTATAA GAATGGAGGC 
GAAAAGATCG GCCCCTTATC TCACAGTGTT GTAACTTCCG TAGCCACCCT AACCTCTCGG 
CCGTCTCCAG TTAGCCCTGG GGATATGCAT ACTGCAAAGT CCTCCAGTTA TGAAGAGAGA 
ATATCAAGTG TCTCCAGCAC TTCAGCCCTG CTGCACACTT CACTGGAAAC AGGAAGCACT 
TCGGAAAGGA GCTTGCTGCT CTCGGAGCCC TGGAGTCCCC CAAGTGTGAA AGATAAAGTA 
TATGAAAAGG AACCGAGGAA ACCAATGTTG GAAGAGACAA GGGTCTCTGC AGCAGAAGAT 
AGCAGCAGCT TCGGAAAATG CAGCTCGCTC CATAAGGAAA AAACCCACCC CGCGCTTGAT 
GTGGCAAAGG AGGAAGAACA TGTTGAGGAG CAGAAAGTGC CTGGGCATGT TGCCTCAAGT 
AGCAGCAGAG TGGAGTTGCT CAGCCCCCAG AAGCTGTCCC AGCCTGCTGA GCATTCAGAA 
TCCCAAGCAA AGCCTGAAAG TGCCCTGCAG GTAGACGCTG AAGATAAGAA GAGCCTACAT 
CCCGAGCCCA GCATGAGAAG AAGGAAAGAG ATCCACGAGA TTGTTGAGCC GGCCTCTGAA 
CTCCTTGGCA CCAGCGCTGC AAAGACCGTT TCTCACACTC CAGCTGCTGC TCCTGCTGCG 
GTGTCCTACG GACCACAGGC ATCAGAGCTA AGTGAGGAAA AGCCCGAGGA GGAAACAGCT 
GAGATCTCCT CCACCATCAG CAACAGGTCC AACGAGGATC TCGATACGCC CGGCAAAGCC 
ATGTCCATCA AAGACAGGCT GGCGCTGCTA AAGAAGAGTG GGGAAGAGGA TTGGAAAAAC 
AGGCTTAACA AAAAGCAGGA GTACAGCAAA GTGTCTGTCA TCGAGCGCAG CACGCTCACC 
CAGCTCCATG AGATTGAGCA GTCGCTGACA AAGAAGGAAC CTGTCTATGC TTCTGTTTAT 
TCTCCTGCTC TACCTGCCGC CCATAAATGC CAGTATTTTG TTCCTATACA TCAGGTCAGT 
AATGCTACGA CCCTGGGGCA GTCCAGCCCT GTGATGTTCG ATGAGCGGCA CCCCTGGAGA 
TGGAAGCAAA GGGTGACTGA CGGCAATGGG CTTCACGTGT CTATTGAAGA AAGGAAGCAG 
CAGATTACAG TCAGGGAGGA GGCCTGGAAA ACCAAAGGCA TAGGGGCAGC AAATGATTCC 
ACACAATTTA CAGTGGCCGG CCGCATGGTT AAGAAAGGTC TGGCCTCACC TACAGCCATT 
ACCCCCGTCG CATCTCCAAT GTCTCTCAAA TTTAAAACTC CAGTTTCCAA GCCTCTGGAA 
GAAATTGAAG CCAAACCTGA TATGAATTTG GAATCTGACA GGAAGTTAGA AAAACTAGAA 
TCATTTTTGG GAAAACTGCA CAACAGGGTT GGAGGGCTAC AAGATACCAC ACTGTCAGTC 
ACAGGCCAAT CAGTGAAGGA GGTGATGAAG GTGGACGATG ATGAAACTTT CGGAAGGTTC 
TACCGCCCTT TTGAATTCTC CACTGCCCAC GTGCCATTTG ATTTTGAAGA TGATTTTGAT 
CTTCTCTTCA GTTCGGAGGA ACCAAAGCTG ACATCTGCTG TAGCAGAACA CAAGCGTGCC 
GTCCGGCCAG CCCGCCGGGT GCAAGCGTCG GGAAACCCAC TGAAGATGCT TGCGGCCAGG 
GAGGACATCC TACAGGAGTA CACAGAGACG CGGCTGAACG TGGCGTTCAT GGAGTCCAAG 
AGAATGAAAG TAGAAAAAAT GTCCAAGAAC TCAAACTTCT CAGAAGTCGC ACTTGCAGGC 
CTGGCAAGCA AAGAAAACTT TACCAGCGTA AGCCTGCGGA GTGTCAATAT GACCGAGCAG 
CACTCCAACA ACAGCGCCGT GCCATACAAG AAGCTTATGC TAGTTCAGAT TAAAGGACGC 
AGGCACGTTC AGTCGAGGCT GGTGGAACCG CGTGCATCCT CTCTGAATAG CGGAGACTGC 
TTCCTGCTGA TCACCCCGCA CCACTGCTTC TTGTGGGTCG GAGAGTTTGC AAACGTCATT 
GAGAAAGCCA AGGCTTCTGA GCTGGCATCA ATAATTCAGA CCAAGAGGGA ACTTGGATGC 
AGGGCATCGT ATGTGCAAAC AGTCGAGGAA GGAATAAATA CCCACACGCA TGGAGCCAAG 
GATTTTTGGA AACTTCTGAG CGGTCAGACT GAATCTCAAG CTGCTGGGAG CCCAGAGGAG 
GATGAGAGAT ATGAAAATGC CATCATAGAA ACAAACTGTA TTTATCGATT GCTGGAAGAT 
AAGCTGGTCC CTGTTGATGA ATTTTGGGGC AAAGTACCTA AGTGTTCACT TTTAGAGTCT 
AAAGAGGTGC TTGTGTTTGA TTTTGGAAGT GAAGTGTATG TGTGGCATGG GAAGGAGGTG 
ACCTTGGCAC AGAGGAAGGT GGCGTTCCAG TTAGCCAAAC ATCTCTGGAA TGGAGCTTTT 
GACTATGAAA ATTGTGATAT CAATCCTCTG GACCCTGGGG AATGCAATTC TCTCATTCCA 
AGGAAAGGGC ACGGGCGCCC TGATTGGGCA ATATTTGGAA GGCTGACAGA GCATAATGAA 
ACCATACTGT TCAAGGAAAA GTTCCTGGAT TGGACAGAAA TGAAGAAACC CAATGAGAAG 
GGTGTGTGCG AGGACTCACC GAAGCTTAAG GAAGACCCAC AATCTGATGT GAAGCCTTAT 
GACATCATGC TGATGGTCCC AGTTCCCCAG AAATCTGTGG GTACGGTTCT TGATGGATTA 
AATGTCGCCC GTGGTTATGG ACTTATTGAG GGAGATGATT GCCGCCAGTA TGAAATCTCA 
ACCGTCTCGG TGGATGTCTG GCATATCCTT GAATTCGACT ACAGCCGGCT GCCGAAGCAG 
AGCATTGGCC AGTTCCACGA GGGAGACGCC TATGTGGTAA AATGGAAGTA CTTTGTGAGC 
ACAACAGTTG GACAGCGCCA GAAAACCGAG CACGTCCGGA TAGCGGGTAA AGAGAAGTGC 
GCCTACTTCT TCTGGCAGGG ACGGAACTCG CCCGTAAGTG AGAAGGGAAC CTCAGCGCTG 
ATGACTGTGG AGCTTGATGA AGAGCGGGGA GCACAGGTCC AGGTGCTTCA GGGAAAAGAG 
CCCCCCTGTT TCTTACAATG TTTCCAGGGA GGAATGATTG TGCATGCTGG GAGACGGGAA 
GAAGAGGAAG AGAACACGCA AAGTGACTGG CGGTTGTACT GTGTACGTGG GGAGGTGCCG 
GTGGAAGGGA ATTTGATGGA AGTCGCTTGT CACTGCAGCA GCCTGCGCTC CAGGACCTCC 
TTGATACTCC TCAGTATAAA TAAAGCGCTC ATCTACCTTT GGCACGGCTG CAAAGTCCAA 
ATCCATGCCA AGGAAGTGGG ACGGACGGCA GCCAATAAAA TAAAAGAGGA GTGCCCCCTG 
GAAGCCGGGC TACACAGCAG CAGTAAGGTG ACCATATATG AATGTGATGA AGGCTCGGAA 
CCCCCCGGAT TCTGGGATGC GCTGGGCAGG CGAGACCGTA AAGCGTACGA CTGCATGTTG 
CAAGATCCTG GGAAATTTAA CTTCACGCCA CGACTGTTCA GCCTCAGTAG TTCATCGGGA 
GAGTTTACAG CATCAGAAAT CCTGTATCCT TCAAGGCATC CGAATGTGGT TAACTCCATG 
CCATTTTTAC AAGAAGACCT GTATACGGCC TCCCAGCCAG CTTTATTCCT CGTTGACAAC 
CATCACGAGG TTTACCTGTG GCAAGGTTGG TGGCCGTCTG AGAATATTAT TACTGGGTCT 
GCTCGTATTC GGTGGGATTC AGACAGAAAA AGCGCAATGG AGACCGTACT CAGCTACTGC 
AAAGGAAAGA ATATCAAGAA ACCTCCCAAG TCGTATCTAA TCCATGCCGG GCTGGAGCCT 
CTGACCTTTA CCAACATGTT TCCAAGCTGG GAACATAGGG AAGACATTGC CCAAATCACA 
GAAAAGGATG CGGAAGTCTC TAACCAGATA ATCCTTGTAG AAGATGTACT CGCTAAACTT 
TGTAAAAGAA TCTACCCCTT GGCAGATCTT CTTGCCCGCC CGTTGCCCGA AGGAGTCGAC 
CCTCTCAAGC TTGAGCTCTA TCTGTCAGAT AAAGACTTTG AGGTTGCGTT AGAAATGACA 
AGAGAAGAAT ACAACACACT GCCGCCCTGG AAACAGGTTA ATACAAAGAA AGCCAAAGGA 
CTTTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821108.1
CDS	12..6980
Translation	MSWEAQCVCPGTCRGRAGWSCVLLKKSRLDFCLRTDVTSEAGFDREGTFQHRTSRLPQKKPPSFQKSFVIEVTGDFTNNSRETDAHLLAVLPKVSELRKLFEPSRRSISEMKRKERIARRLEGIDNDPQPIMLQTRLLEEDMPRYMRATDAYNPHIGHANEDTASESSIETHSRWQNRWQPSTESSSMSGQMAVEETESKAERIARYKAERRRQLAERYGVPMDSEADSEYQSRYTRIRKDGEISEKKSVLEEDEIKENSSDYLYRQDIKEPRRFRMYESKHRDINENVSERDALKNVENNNNQYREISELDGSTFSCMGQAITVGEAPRSPKFPRRASEKSLQLSSSPPSEQLLLSDSRQGTGKQAPEWSLQKDSDGDTASLISWPSRVKVRERLGREHIHGFPKTDTEISTQRKHPPGPAHPVPFQSETSAFHRVYKQPALVHQPIHGYIQPASVAHTPKIVAPHAPDSSVMVSAFTTSLKNPRSSSDVYIEKSHHAFQGQDNIVEPCKQVISNGKSHSMYEEKPTYISSASKPRNETLPGHKDPDLHVSHSLPLQRQTSGNFGKSEKVLFLQSGRSQDHTKEVSTMKHKMLTRSMSDTTNSQKMEAFKIKDSDVQKEMVFLPSEDHVAIPNGEAGIVNTKVSVAQLRSAYLETASHAQKSEFEPKAEMPTSGALSATTERERGPRRPRLYFSPGESRKTSERFRTQPVTSAERNQMDRSSFQSPEQIAEEEAKVDERAKLSVAAKRLLFREMEKSLESKPAKPRARNAAVERRLRRAQDRSRTQPVTSEEVVIAATEPSAASHTVTTHTALPRIPSPTVTKSSVHHISLQASSHKSTISKQEEIQKEIHDDQSIPEEPDNSSLSLAEKMALFNKLSQPITKEVSTRSRMDIRQRRMNARYQTQPVTLVEVEQYKNGGEKIGPLSHSVVTSVATLTSRPSPVSPGDMHTAKSSSYEERISSVSSTSALLHTSLETGSTSERSLLLSEPWSPPSVKDKVYEKEPRKPMLEETRVSAAEDSSSFGKCSSLHKEKTHPALDVAKEEEHVEEQKVPGHVASSSSRVELLSPQKLSQPAEHSESQAKPESALQVDAEDKKSLHPEPSMRRRKEIHEIVEPASELLGTSAAKTVSHTPAAAPAAVSYGPQASELSEEKPEEETAEISSTISNRSNEDLDTPGKAMSIKDRLALLKKSGEEDWKNRLNKKQEYSKVSVIERSTLTQLHEIEQSLTKKEPVYASVYSPALPAAHKCQYFVPIHQVSNATTLGQSSPVMFDERHPWRWKQRVTDGNGLHVSIEERKQQITVREEAWKTKGIGAANDSTQFTVAGRMVKKGLASPTAITPVASPMSLKFKTPVSKPLEEIEAKPDMNLESDRKLEKLESFLGKLHNRVGGLQDTTLSVTGQSVKEVMKVDDDETFGRFYRPFEFSTAHVPFDFEDDFDLLFSSEEPKLTSAVAEHKRAVRPARRVQASGNPLKMLAAREDILQEYTETRLNVAFMESKRMKVEKMSKNSNFSEVALAGLASKENFTSVSLRSVNMTEQHSNNSAVPYKKLMLVQIKGRRHVQSRLVEPRASSLNSGDCFLLITPHHCFLWVGEFANVIEKAKASELASIIQTKRELGCRASYVQTVEEGINTHTHGAKDFWKLLSGQTESQAAGSPEEDERYENAIIETNCIYRLLEDKLVPVDEFWGKVPKCSLLESKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGAFDYENCDINPLDPGECNSLIPRKGHGRPDWAIFGRLTEHNETILFKEKFLDWTEMKKPNEKGVCEDSPKLKEDPQSDVKPYDIMLMVPVPQKSVGTVLDGLNVARGYGLIEGDDCRQYEISTVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKYFVSTTVGQRQKTEHVRIAGKEKCAYFFWQGRNSPVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMIVHAGRREEEEENTQSDWRLYCVRGEVPVEGNLMEVACHCSSLRSRTSLILLSINKALIYLWHGCKVQIHAKEVGRTAANKIKEECPLEAGLHSSSKVTIYECDEGSEPPGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFTASEILYPSRHPNVVNSMPFLQEDLYTASQPALFLVDNHHEVYLWQGWWPSENIITGSARIRWDSDRKSAMETVLSYCKGKNIKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAQITEKDAEVSNQIILVEDVLAKLCKRIYPLADLLARPLPEGVDPLKLELYLSDKDFEVALEMTREEYNTLPPWKQVNTKKAKGLF

Target ORF information:

RefSeq Version	XM_012965654.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis supervillin (svil), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965654.2

ORF Insert Sequence:

ATGAGCTGGG AAGCACAGTG CGTGTGCCCG GGTACGTGTC GGGGGCGCGC AGGCTGGTCT 
TGTGTTTTAC TGAAAAAGTC CCGTTTGGAT TTCTGCCTTA GAACTGACGT CACTTCAGAA 
GCAGGATTTG ATCGAGAAGG TACATTTCAA CACCGAACGT CACGTCTTCC ACAGAAAAAG 
CCGCCAAGTT TTCAGAAGAG TTTTGTTATT GAAGTTACTG GGGATTTCAC AAACAACTCC 
AGGGAAACTG ATGCACATCT GCTAGCTGTA CTTCCAAAAG TTTCAGAACT AAGGAAACTC 
TTTGAGCCAA GCAGGAGATC CATTTCAGAG ATGAAAAGAA AAGAAAGAAT AGCCAGACGT 
TTGGAAGGAA TTGATAACGA CCCACAACCA ATTATGCTAC AGACACGTTT ATTGGAAGAA 
GATATGCCAC GATATATGCG CGCAACAGAT GCATATAATC CACACATAGG TCATGCAAAT 
GAAGACACTG CCTCAGAGTC ATCAATAGAA ACACATTCTA GATGGCAAAA CCGCTGGCAG 
CCAAGCACTG AATCATCTTC TATGTCAGGT CAAATGGCGG TAGAGGAAAC AGAATCAAAA 
GCAGAAAGGA TAGCTCGATA CAAGGCGGAG CGAAGACGGC AATTGGCAGA AAGATATGGT 
GTCCCTATGG ATTCCGAAGC GGATTCCGAA TACCAGTCAA GGTACACGAG AATAAGGAAA 
GACGGTGAGA TCTCTGAAAA GAAATCTGTG TTAGAGGAAG ATGAAATTAA AGAGAATAGC 
TCAGATTATT TATACCGTCA AGACATTAAG GAGCCTCGCA GATTTCGAAT GTACGAATCA 
AAGCATCGGG ACATTAATGA GAATGTGTCG GAGAGAGATG CTCTCAAAAA TGTGGAAAAC 
AATAATAATC AATATCGAGA AATATCCGAG TTGGATGGAT CTACCTTTTC GTGCATGGGA 
CAAGCCATCA CAGTTGGGGA AGCACCCAGG TCTCCAAAGT TCCCTCGTCG TGCATCAGAG 
AAATCACTTC AGCTTTCCTC CTCTCCACCA TCTGAACAGC TACTGCTTAG CGACTCCAGA 
CAAGGAACAG GAAAACAGGC ACCAGAGTGG TCTCTCCAAA AAGATTCCGA TGGGGACACA 
GCGTCTCTCA TCAGCTGGCC GTCCAGAGTT AAAGTTAGAG AGAGGCTGGG GAGAGAACAT 
ATTCACGGAT TTCCCAAAAC TGACACAGAG ATCTCAACGC AAAGGAAACA TCCACCCGGG 
CCTGCCCACC CTGTTCCTTT CCAGTCGGAA ACCTCTGCTT TTCATAGGGT TTATAAACAG 
CCGGCATTAG TTCACCAGCC CATTCATGGC TACATCCAGC CTGCTAGTGT TGCTCATACA 
CCTAAAATTG TGGCACCTCA TGCACCTGAT TCGAGTGTCA TGGTGTCCGC TTTTACTACC 
TCCTTAAAAA ATCCCAGATC TTCCAGTGAT GTGTATATTG AGAAATCCCA CCATGCATTT 
CAAGGTCAGG ACAATATCGT AGAGCCATGC AAGCAGGTGA TCAGCAATGG CAAATCGCAC 
AGTATGTATG AAGAGAAGCC AACATATATT TCATCTGCCA GCAAGCCAAG AAACGAGACC 
TTGCCTGGGC ATAAAGACCC AGACCTGCAT GTTTCACACA GTTTACCACT TCAGAGACAA 
ACCTCTGGAA ACTTTGGGAA AAGCGAAAAG GTCTTGTTCC TTCAGAGTGG CAGATCCCAG 
GACCATACCA AGGAAGTATC TACGATGAAG CATAAAATGC TGACCCGTTC AATGTCTGAC 
ACCACCAACT CCCAAAAGAT GGAAGCTTTT AAGATAAAGG ACTCCGATGT CCAGAAAGAG 
ATGGTGTTTT TGCCTTCTGA AGACCATGTT GCCATTCCAA ATGGAGAAGC CGGCATTGTC 
AATACCAAAG TGTCAGTTGC TCAGCTTAGG AGTGCATATT TGGAGACGGC CAGCCACGCA 
CAAAAATCTG AGTTTGAACC TAAGGCTGAA ATGCCAACGT CAGGTGCTTT GTCTGCTACT 
ACTGAGCGGG AAAGAGGCCC TCGAAGGCCG AGACTTTATT TCTCTCCTGG TGAAAGTAGA 
AAGACCTCTG AAAGATTTAG AACCCAGCCT GTAACGTCAG CTGAGCGAAA TCAGATGGAT 
AGATCAAGCT TCCAGTCACC AGAGCAGATT GCTGAAGAAG AAGCAAAAGT GGATGAAAGA 
GCCAAACTTA GCGTGGCTGC GAAAAGGCTT CTATTCAGGG AAATGGAAAA ATCTCTGGAG 
TCGAAACCAG CCAAGCCACG TGCCAGAAAT GCAGCAGTGG AGAGGAGGCT TCGCCGAGCA 
CAAGACAGGT CTCGGACGCA ACCCGTTACC AGTGAAGAGG TGGTCATTGC TGCTACTGAG 
CCTAGTGCTG CTTCGCACAC TGTGACCACA CACACGGCCC TCCCTCGAAT ACCTAGCCCC 
ACTGTAACTA AGAGCTCTGT ACACCATATC AGTTTGCAGG CATCATCCCA TAAAAGCACA 
ATATCAAAGC AAGAGGAGAT ACAAAAGGAA ATACACGATG ACCAGAGCAT TCCCGAAGAG 
CCTGATAATT CCTCGCTTTC CTTGGCTGAA AAAATGGCAT TATTTAATAA GCTTTCCCAG 
CCAATAACAA AAGAGGTGTC TACGAGGAGC AGAATGGATA TTCGACAAAG GCGAATGAAT 
GCGCGTTACC AGACTCAGCC CGTCACCCTT GTGGAGGTTG AACAGTATAA GAATGGAGGC 
GAAAAGATCG GCCCCTTATC TCACAGTGTT GTAACTTCCG TAGCCACCCT AACCTCTCGG 
CCGTCTCCAG TTAGCCCTGG GGATATGCAT ACTGCAAAGT CCTCCAGTTA TGAAGAGAGA 
ATATCAAGTG TCTCCAGCAC TTCAGCCCTG CTGCACACTT CACTGGAAAC AGGAAGCACT 
TCGGAAAGGA GCTTGCTGCT CTCGGAGCCC TGGAGTCCCC CAAGTGTGAA AGATAAAGTA 
TATGAAAAGG AACCGAGGAA ACCAATGTTG GAAGAGACAA GGGTCTCTGC AGCAGAAGAT 
AGCAGCAGCT TCGGAAAATG CAGCTCGCTC CATAAGGAAA AAACCCACCC CGCGCTTGAT 
GTGGCAAAGG AGGAAGAACA TGTTGAGGAG CAGAAAGTGC CTGGGCATGT TGCCTCAAGT 
AGCAGCAGAG TGGAGTTGCT CAGCCCCCAG AAGCTGTCCC AGCCTGCTGA GCATTCAGAA 
TCCCAAGCAA AGCCTGAAAG TGCCCTGCAG GTAGACGCTG AAGATAAGAA GAGCCTACAT 
CCCGAGCCCA GCATGAGAAG AAGGAAAGAG ATCCACGAGA TTGTTGAGCC GGCCTCTGAA 
CTCCTTGGCA CCAGCGCTGC AAAGACCGTT TCTCACACTC CAGCTGCTGC TCCTGCTGCG 
GTGTCCTACG GACCACAGGC ATCAGAGCTA AGTGAGGAAA AGCCCGAGGA GGAAACAGCT 
GAGATCTCCT CCACCATCAG CAACAGGTCC AACGAGGATC TCGATACGCC CGGCAAAGCC 
ATGTCCATCA AAGACAGGCT GGCGCTGCTA AAGAAGAGTG GGGAAGAGGA TTGGAAAAAC 
AGGCTTAACA AAAAGCAGGA GTACAGCAAA GTGTCTGTCA TCGAGCGCAG CACGCTCACC 
CAGCTCCATG AGATTGAGCA GTCGCTGACA AAGAAGGAAC CTGTCTATGC TTCTGTTTAT 
TCTCCTGCTC TACCTGCCGC CCATAAATGC CAGTATTTTG TTCCTATACA TCAGGTCAGT 
AATGCTACGA CCCTGGGGCA GTCCAGCCCT GTGATGTTCG ATGAGCGGCA CCCCTGGAGA 
TGGAAGCAAA GGGTGACTGA CGGCAATGGG CTTCACGTGT CTATTGAAGA AAGGAAGCAG 
CAGATTACAG TCAGGGAGGA GGCCTGGAAA ACCAAAGGCA TAGGGGCAGC AAATGATTCC 
ACACAATTTA CAGTGGCCGG CCGCATGGTT AAGAAAGGTC TGGCCTCACC TACAGCCATT 
ACCCCCGTCG CATCTCCAAT GTCTCTCAAA TTTAAAACTC CAGTTTCCAA GCCTCTGGAA 
GAAATTGAAG CCAAACCTGA TATGAATTTG GAATCTGACA GGAAGTTAGA AAAACTAGAA 
TCATTTTTGG GAAAACTGCA CAACAGGGTT GGAGGGCTAC AAGATACCAC ACTGTCAGTC 
ACAGGCCAAT CAGTGAAGGA GGTGATGAAG GTGGACGATG ATGAAACTTT CGGAAGGTTC 
TACCGCCCTT TTGAATTCTC CACTGCCCAC GTGCCATTTG ATTTTGAAGA TGATTTTGAT 
CTTCTCTTCA GTTCGGAGGA ACCAAAGCTG ACATCTGCTG TAGCAGAACA CAAGCGTGCC 
GTCCGGCCAG CCCGCCGGGT GCAAGCGTCG GGAAACCCAC TGAAGATGCT TGCGGCCAGG 
GAGGACATCC TACAGGAGTA CACAGAGACG CGGCTGAACG TGGCGTTCAT GGAGTCCAAG 
AGAATGAAAG TAGAAAAAAT GTCCAAGAAC TCAAACTTCT CAGAAGTCGC ACTTGCAGGC 
CTGGCAAGCA AAGAAAACTT TACCAGCGTA AGCCTGCGGA GTGTCAATAT GACCGAGCAG 
CACTCCAACA ACAGCGCCGT GCCATACAAG AAGCTTATGC TAGTTCAGAT TAAAGGACGC 
AGGCACGTTC AGTCGAGGCT GGTGGAACCG CGTGCATCCT CTCTGAATAG CGGAGACTGC 
TTCCTGCTGA TCACCCCGCA CCACTGCTTC TTGTGGGTCG GAGAGTTTGC AAACGTCATT 
GAGAAAGCCA AGGCTTCTGA GCTGGCATCA ATAATTCAGA CCAAGAGGGA ACTTGGATGC 
AGGGCATCGT ATGTGCAAAC AGTCGAGGAA GGAATAAATA CCCACACGCA TGGAGCCAAG 
GATTTTTGGA AACTTCTGAG CGGTCAGACT GAATCTCAAG CTGCTGGGAG CCCAGAGGAG 
GATGAGAGAT ATGAAAATGC CATCATAGAA ACAAACTGTA TTTATCGATT GCTGGAAGAT 
AAGCTGGTCC CTGTTGATGA ATTTTGGGGC AAAGTACCTA AGTGTTCACT TTTAGAGTCT 
AAAGAGGTGC TTGTGTTTGA TTTTGGAAGT GAAGTGTATG TGTGGCATGG GAAGGAGGTG 
ACCTTGGCAC AGAGGAAGGT GGCGTTCCAG TTAGCCAAAC ATCTCTGGAA TGGAGCTTTT 
GACTATGAAA ATTGTGATAT CAATCCTCTG GACCCTGGGG AATGCAATTC TCTCATTCCA 
AGGAAAGGGC ACGGGCGCCC TGATTGGGCA ATATTTGGAA GGCTGACAGA GCATAATGAA 
ACCATACTGT TCAAGGAAAA GTTCCTGGAT TGGACAGAAA TGAAGAAACC CAATGAGAAG 
GGTGTGTGCG AGGACTCACC GAAGCTTAAG GAAGACCCAC AATCTGATGT GAAGCCTTAT 
GACATCATGC TGATGGTCCC AGTTCCCCAG AAATCTGTGG GTACGGTTCT TGATGGATTA 
AATGTCGCCC GTGGTTATGG ACTTATTGAG GGAGATGATT GCCGCCAGTA TGAAATCTCA 
ACCGTCTCGG TGGATGTCTG GCATATCCTT GAATTCGACT ACAGCCGGCT GCCGAAGCAG 
AGCATTGGCC AGTTCCACGA GGGAGACGCC TATGTGGTAA AATGGAAGTA CTTTGTGAGC 
ACAACAGTTG GACAGCGCCA GAAAACCGAG CACGTCCGGA TAGCGGGTAA AGAGAAGTGC 
GCCTACTTCT TCTGGCAGGG ACGGAACTCG CCCGTAAGTG AGAAGGGAAC CTCAGCGCTG 
ATGACTGTGG AGCTTGATGA AGAGCGGGGA GCACAGGTCC AGGTGCTTCA GGGAAAAGAG 
CCCCCCTGTT TCTTACAATG TTTCCAGGGA GGAATGATTG TGCATGCTGG GAGACGGGAA 
GAAGAGGAAG AGAACACGCA AAGTGACTGG CGGTTGTACT GTGTACGTGG GGAGGTGCCG 
GTGGAAGGGA ATTTGATGGA AGTCGCTTGT CACTGCAGCA GCCTGCGCTC CAGGACCTCC 
TTGATACTCC TCAGTATAAA TAAAGCGCTC ATCTACCTTT GGCACGGCTG CAAAGTCCAA 
ATCCATGCCA AGGAAGTGGG ACGGACGGCA GCCAATAAAA TAAAAGAGGA GTGCCCCCTG 
GAAGCCGGGC TACACAGCAG CAGTAAGGTG ACCATATATG AATGTGATGA AGGCTCGGAA 
CCCCCCGGAT TCTGGGATGC GCTGGGCAGG CGAGACCGTA AAGCGTACGA CTGCATGTTG 
CAAGATCCTG GGAAATTTAA CTTCACGCCA CGACTGTTCA GCCTCAGTAG TTCATCGGGA 
GAGTTTACAG CATCAGAAAT CCTGTATCCT TCAAGGCATC CGAATGTGGT TAACTCCATG 
CCATTTTTAC AAGAAGACCT GTATACGGCC TCCCAGCCAG CTTTATTCCT CGTTGACAAC 
CATCACGAGG TTTACCTGTG GCAAGGTTGG TGGCCGTCTG AGAATATTAT TACTGGGTCT 
GCTCGTATTC GGTGGGATTC AGACAGAAAA AGCGCAATGG AGACCGTACT CAGCTACTGC 
AAAGGAAAGA ATATCAAGAA ACCTCCCAAG TCGTATCTAA TCCATGCCGG GCTGGAGCCT 
CTGACCTTTA CCAACATGTT TCCAAGCTGG GAACATAGGG AAGACATTGC CCAAATCACA 
GAAAAGGATG CGGAAGTCTC TAACCAGATA ATCCTTGTAG AAGATGTACT CGCTAAACTT 
TGTAAAAGAA TCTACCCCTT GGCAGATCTT CTTGCCCGCC CGTTGCCCGA AGGAGTCGAC 
CCTCTCAAGC TTGAGCTCTA TCTGTCAGAT AAAGACTTTG AGGTTGCGTT AGAAATGACA 
AGAGAAGAAT ACAACACACT GCCGCCCTGG AAACAGGTTA ATACAAAGAA AGCCAAAGGA 
CTTTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

svil ( XM_012965659.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821113.1 Xenopus tropicalis supervillin (svil), transcript variant X4, mRNA.

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CloneID	OXa29954
Clone ID Related Accession (Same CDS sequence)	XM_012965659.1 , XM_012965655.2
Accession Version	XM_012965659.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	supervillin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT AGCCAGACGT TTGGAAGGAA TTGATAACGA CCCACAACCA 
ATTATGCTAC AGACACGTTT ATTGGAAGAA GATATGCCAC GATATATGCG CGCAACAGAT 
GCATATAATC CACACATAGG TCATGCAAAT GAAGACACTG CCTCAGAGTC ATCAATAGAA 
ACACATTCTA GATGGCAAAA CCGCTGGCAG CCAAGCACTG AATCATCTTC TATGTCAGGT 
CAAATGGCGG TAGAGGAAAC AGAATCAAAA GCAGAAAGGA TAGCTCGATA CAAGGCGGAG 
CGAAGACGGC AATTGGCAGA AAGATATGGT GTCCCTATGG ATTCCGAAGC GGATTCCGAA 
TACCAGTCAA GGTACACGAG AATAAGGAAA GACGGTGAGA TCTCTGAAAA GAAATCTGTG 
TTAGAGGAAG ATGAAATTAA AGAGAATAGC TCAGATTATT TATACCGTCA AGACATTAAG 
GAGCCTCGCA GATTTCGAAT GTACGAATCA AAGCATCGGG ACATTAATGA GAATGTGTCG 
GAGAGAGATG CTCTCAAAAA TGTGGAAAAC AATAATAATC AATATCGAGA AATATCCGAG 
TTGGATGGAT CTACCTTTTC GTGCATGGGA CAAGCCATCA CAGTTGGGGA AGCACCCAGG 
TCTCCAAAGT TCCCTCGTCG TGCATCAGAG AAATCACTTC AGCTTTCCTC CTCTCCACCA 
TCTGAACAGC TACTGCTTAG CGACTCCAGA CAAGGAACAG GAAAACAGGC ACCAGAGTGG 
TCTCTCCAAA AAGATTCCGA TGGGGACACA GCGTCTCTCA TCAGCTGGCC GTCCAGAGTT 
AAAGTTAGAG AGAGGCTGGG GAGAGAACAT ATTCACGGAT TTCCCAAAAC TGACACAGAG 
ATCTCAACGC AAAGGAAACA TCCACCCGGG CCTGCCCACC CTGTTCCTTT CCAGTCGGAA 
ACCTCTGCTT TTCATAGGGT TTATAAACAG CCGGCATTAG TTCACCAGCC CATTCATGGC 
TACATCCAGC CTGCTAGTGT TGCTCATACA CCTAAAATTG TGGCACCTCA TGCACCTGAT 
TCGAGTGTCA TGGTGTCCGC TTTTACTACC TCCTTAAAAA ATCCCAGATC TTCCAGTGAT 
GTGTATATTG AGAAATCCCA CCATGCATTT CAAGGTCAGG ACAATATCGT AGAGCCATGC 
AAGCAGGTGA TCAGCAATGG CAAATCGCAC AGTATGTATG AAGAGAAGCC AACATATATT 
TCATCTGCCA GCAAGCCAAG AAACGAGACC TTGCCTGGGC ATAAAGACCC AGACCTGCAT 
GTTTCACACA GTTTACCACT TCAGAGACAA ACCTCTGGAA ACTTTGGGAA AAGCGAAAAG 
GTCTTGTTCC TTCAGAGTGG CAGATCCCAG GACCATACCA AGGAAGTATC TACGATGAAG 
CATAAAATGC TGACCCGTTC AATGTCTGAC ACCACCAACT CCCAAAAGAT GGAAGCTTTT 
AAGATAAAGG ACTCCGATGT CCAGAAAGAG ATGGTGTTTT TGCCTTCTGA AGACCATGTT 
GCCATTCCAA ATGGAGAAGC CGGCATTGTC AATACCAAAG TGTCAGTTGC TCAGCTTAGG 
AGTGCATATT TGGAGACGGC CAGCCACGCA CAAAAATCTG AGTTTGAACC TAAGGCTGAA 
ATGCCAACGT CAGGTGCTTT GTCTGCTACT ACTGAGCGGG AAAGAGGCCC TCGAAGGCCG 
AGACTTTATT TCTCTCCTGG TGAAAGTAGA AAGACCTCTG AAAGATTTAG AACCCAGCCT 
GTAACGTCAG CTGAGCGAAA TCAGATGGAT AGATCAAGCT TCCAGTCACC AGAGCAGATT 
GCTGAAGAAG AAGCAAAAGT GGATGAAAGA GCCAAACTTA GCGTGGCTGC GAAAAGGCTT 
CTATTCAGGG AAATGGAAAA ATCTCTGGAG TCGAAACCAG CCAAGCCACG TGCCAGAAAT 
GCAGCAGTGG AGAGGAGGCT TCGCCGAGCA CAAGACAGGT CTCGGACGCA ACCCGTTACC 
AGTGAAGAGG TGGTCATTGC TGCTACTGAG CCTAGTGCTG CTTCGCACAC TGTGACCACA 
CACACGGCCC TCCCTCGAAT ACCTAGCCCC ACTGTAACTA AGAGCTCTGT ACACCATATC 
AGTTTGCAGG CATCATCCCA TAAAAGCACA ATATCAAAGC AAGAGGAGAT ACAAAAGGAA 
ATACACGATG ACCAGAGCAT TCCCGAAGAG CCTGATAATT CCTCGCTTTC CTTGGCTGAA 
AAAATGGCAT TATTTAATAA GCTTTCCCAG CCAATAACAA AAGAGGTGTC TACGAGGAGC 
AGAATGGATA TTCGACAAAG GCGAATGAAT GCGCGTTACC AGACTCAGCC CGTCACCCTT 
GTGGAGGTTG AACAGTATAA GAATGGAGGC GAAAAGATCG GCCCCTTATC TCACAGTGTT 
GTAACTTCCG TAGCCACCCT AACCTCTCGG CCGTCTCCAG TTAGCCCTGG GGATATGCAT 
ACTGCAAAGT CCTCCAGTTA TGAAGAGAGA ATATCAAGTG TCTCCAGCAC TTCAGCCCTG 
CTGCACACTT CACTGGAAAC AGGAAGCACT TCGGAAAGGA GCTTGCTGCT CTCGGAGCCC 
TGGAGTCCCC CAAGTGTGAA AGATAAAGTA TATGAAAAGG AACCGAGGAA ACCAATGTTG 
GAAGAGACAA GGGTCTCTGC AGCAGAAGAT AGCAGCAGCT TCGGAAAATG CAGCTCGCTC 
CATAAGGAAA AAACCCACCC CGCGCTTGAT GTGGCAAAGG AGGAAGAACA TGTTGAGGAG 
CAGAAAGTGC CTGGGCATGT TGCCTCAAGT AGCAGCAGAG TGGAGTTGCT CAGCCCCCAG 
AAGCTGTCCC AGCCTGCTGA GCATTCAGAA TCCCAAGCAA AGCCTGAAAG TGCCCTGCAG 
GTAGACGCTG AAGATAAGAA GAGCCTACAT CCCGAGCCCA GCATGAGAAG AAGGAAAGAG 
ATCCACGAGA TTGTTGAGCC GGCCTCTGAA CTCCTTGGCA CCAGCGCTGC AAAGACCGTT 
TCTCACACTC CAGCTGCTGC TCCTGCTGCG GTGTCCTACG GACCACAGGC ATCAGAGCTA 
AGTGAGGAAA AGCCCGAGGA GGAAACAGCT GAGATCTCCT CCACCATCAG CAACAGGTCC 
AACGAGGATC TCGATACGCC CGGCAAAGCC ATGTCCATCA AAGACAGGCT GGCGCTGCTA 
AAGAAGAGTG GGGAAGAGGA TTGGAAAAAC AGGCTTAACA AAAAGCAGGA GTACAGCAAA 
GTGTCTGTCA TCGAGCGCAG CACGCTCACC CAGCTCCATG AGATTGAGCA GTCGCTGACA 
AAGAAGGAAC CTGTCTATGC TTCTGTTTAT TCTCCTGCTC TACCTGCCGC CCATAAATGC 
CAGTATTTTG TTCCTATACA TCAGGTCAGT AATGCTACGA CCCTGGGGCA GTCCAGCCCT 
GTGATGTTCG ATGAGCGGCA CCCCTGGAGA TGGAAGCAAA GGGTGACTGA CGGCAATGGG 
CTTCACGTGT CTATTGAAGA AAGGAAGCAG CAGATTACAG TCAGGGAGGA GGCCTGGAAA 
ACCAAAGGCA TAGGGGCAGC AAATGATTCC ACACAATTTA CAGTGGCCGG CCGCATGGTT 
AAGAAAGGTC TGGCCTCACC TACAGCCATT ACCCCCGTCG CATCTCCAAT GTCTCTCAAA 
TTTAAAACTC CAGTTTCCAA GCCTCTGGAA GAAATTGAAG CCAAACCTGA TATGAATTTG 
GAATCTGACA GGAAGTTAGA AAAACTAGAA TCATTTTTGG GAAAACTGCA CAACAGGGTT 
GGAGGGCTAC AAGATACCAC ACTGTCAGTC ACAGGCCAAT CAGTGAAGGA GGTGATGAAG 
GTGGACGATG ATGAAACTTT CGGAAGGTTC TACCGCCCTT TTGAATTCTC CACTGCCCAC 
GTGCCATTTG ATTTTGAAGA TGATTTTGAT CTTCTCTTCA GTTCGGAGGA ACCAAAGCTG 
ACATCTGCTG TAGCAGAACA CAAGCGTGCC GTCCGGCCAG CCCGCCGGGT GCAAGCGTCG 
GGAAACCCAC TGAAGATGCT TGCGGCCAGG GAGGACATCC TACAGGAGTA CACAGAGACG 
CGGCTGAACG TGGCGTTCAT GGAGTCCAAG AGAATGAAAG TAGAAAAAAT GTCCAAGAAC 
TCAAACTTCT CAGAAGTCGC ACTTGCAGGC CTGGCAAGCA AAGAAAACTT TACCAGCGTA 
AGCCTGCGGA GTGTCAATAT GACCGAGCAG CACTCCAACA ACAGCGCCGT GCCATACAAG 
AAGCTTATGC TAGTTCAGAT TAAAGGACGC AGGCACGTTC AGTCGAGGCT GGTGGAACCG 
CGTGCATCCT CTCTGAATAG CGGAGACTGC TTCCTGCTGA TCACCCCGCA CCACTGCTTC 
TTGTGGGTCG GAGAGTTTGC AAACGTCATT GAGAAAGCCA AGGCTTCTGA GCTGGCATCA 
ATAATTCAGA CCAAGAGGGA ACTTGGATGC AGGGCATCGT ATGTGCAAAC AGTCGAGGAA 
GGAATAAATA CCCACACGCA TGGAGCCAAG GATTTTTGGA AACTTCTGAG CGGTCAGACT 
GAATCTCAAG CTGCTGGGAG CCCAGAGGAG GATGAGAGAT ATGAAAATGC CATCATAGAA 
ACAAACTGTA TTTATCGATT GCTGGAAGAT AAGCTGGTCC CTGTTGATGA ATTTTGGGGC 
AAAGTACCTA AGTGTTCACT TTTAGAGTCT AAAGAGGTGC TTGTGTTTGA TTTTGGAAGT 
GAAGTGTATG TGTGGCATGG GAAGGAGGTG ACCTTGGCAC AGAGGAAGGT GGCGTTCCAG 
TTAGCCAAAC ATCTCTGGAA TGGAGCTTTT GACTATGAAA ATTGTGATAT CAATCCTCTG 
GACCCTGGGG AATGCAATTC TCTCATTCCA AGGAAAGGGC ACGGGCGCCC TGATTGGGCA 
ATATTTGGAA GGCTGACAGA GCATAATGAA ACCATACTGT TCAAGGAAAA GTTCCTGGAT 
TGGACAGAAA TGAAGAAACC CAATGAGAAG GGTGTGTGCG AGGACTCACC GAAGCTTAAG 
GAAGACCCAC AATCTGATGT GAAGCCTTAT GACATCATGC TGATGGTCCC AGTTCCCCAG 
AAATCTGTGG GTACGGTTCT TGATGGATTA AATGTCGCCC GTGGTTATGG ACTTATTGAG 
GGAGATGATT GCCGCCAGTA TGAAATCTCA ACCGTCTCGG TGGATGTCTG GCATATCCTT 
GAATTCGACT ACAGCCGGCT GCCGAAGCAG AGCATTGGCC AGTTCCACGA GGGAGACGCC 
TATGTGGTAA AATGGAAGTA CTTTGTGAGC ACAACAGTTG GACAGCGCCA GAAAACCGAG 
CACGTCCGGA TAGCGGGTAA AGAGAAGTGC GCCTACTTCT TCTGGCAGGG ACGGAACTCG 
CCCGTAAGTG AGAAGGGAAC CTCAGCGCTG ATGACTGTGG AGCTTGATGA AGAGCGGGGA 
GCACAGGTCC AGGTGCTTCA GGGAAAAGAG CCCCCCTGTT TCTTACAATG TTTCCAGGGA 
GGAATGATTG TGCATGCTGG GAGACGGGAA GAAGAGGAAG AGAACACGCA AAGTGACTGG 
CGGTTGTACT GTGTACGTGG GGAGGTGCCG GTGGAAGGGA ATTTGATGGA AGTCGCTTGT 
CACTGCAGCA GCCTGCGCTC CAGGACCTCC TTGATACTCC TCAGTATAAA TAAAGCGCTC 
ATCTACCTTT GGCACGGCTG CAAAGTCCAA ATCCATGCCA AGGAAGTGGG ACGGACGGCA 
GCCAATAAAA TAAAAGAGGA GTGCCCCCTG GAAGCCGGGC TACACAGCAG CAGTAAGGTG 
ACCATATATG AATGTGATGA AGGCTCGGAA CCCCCCGGAT TCTGGGATGC GCTGGGCAGG 
CGAGACCGTA AAGCGTACGA CTGCATGTTG CAAGATCCTG GGAAATTTAA CTTCACGCCA 
CGACTGTTCA GCCTCAGTAG TTCATCGGGA GAGTTTACAG CATCAGAAAT CCTGTATCCT 
TCAAGGCATC CGAATGTGGT TAACTCCATG CCATTTTTAC AAGAAGACCT GTATACGGCC 
TCCCAGCCAG CTTTATTCCT CGTTGACAAC CATCACGAGG TTTACCTGTG GCAAGGTTGG 
TGGCCGTCTG AGAATATTAT TACTGGGTCT GCTCGTATTC GGTGGGATTC AGACAGAAAA 
AGCGCAATGG AGACCGTACT CAGCTACTGC AAAGGAAAGA ATATCAAGAA ACCTCCCAAG 
TCGTATCTAA TCCATGCCGG GCTGGAGCCT CTGACCTTTA CCAACATGTT TCCAAGCTGG 
GAACATAGGG AAGACATTGC CCAAATCACA GAAAAGGATG CGGAAGTCTC TAACCAGATA 
ATCCTTGTAG AAGATGTACT CGCTAAACTT TGTAAAAGAA TCTACCCCTT GGCAGATCTT 
CTTGCCCGCC CGTTGCCCGA AGGAGTCGAC CCTCTCAAGC TTGAGCTCTA TCTGTCAGAT 
AAAGACTTTG AGGTTGCGTT AGAAATGACA AGAGAAGAAT ACAACACACT GCCGCCCTGG 
AAACAGGTTA ATACAAAGAA AGCCAAAGGA CTTTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821113.1
CDS	221..6859
Translation	MKRKERIARRLEGIDNDPQPIMLQTRLLEEDMPRYMRATDAYNPHIGHANEDTASESSIETHSRWQNRWQPSTESSSMSGQMAVEETESKAERIARYKAERRRQLAERYGVPMDSEADSEYQSRYTRIRKDGEISEKKSVLEEDEIKENSSDYLYRQDIKEPRRFRMYESKHRDINENVSERDALKNVENNNNQYREISELDGSTFSCMGQAITVGEAPRSPKFPRRASEKSLQLSSSPPSEQLLLSDSRQGTGKQAPEWSLQKDSDGDTASLISWPSRVKVRERLGREHIHGFPKTDTEISTQRKHPPGPAHPVPFQSETSAFHRVYKQPALVHQPIHGYIQPASVAHTPKIVAPHAPDSSVMVSAFTTSLKNPRSSSDVYIEKSHHAFQGQDNIVEPCKQVISNGKSHSMYEEKPTYISSASKPRNETLPGHKDPDLHVSHSLPLQRQTSGNFGKSEKVLFLQSGRSQDHTKEVSTMKHKMLTRSMSDTTNSQKMEAFKIKDSDVQKEMVFLPSEDHVAIPNGEAGIVNTKVSVAQLRSAYLETASHAQKSEFEPKAEMPTSGALSATTERERGPRRPRLYFSPGESRKTSERFRTQPVTSAERNQMDRSSFQSPEQIAEEEAKVDERAKLSVAAKRLLFREMEKSLESKPAKPRARNAAVERRLRRAQDRSRTQPVTSEEVVIAATEPSAASHTVTTHTALPRIPSPTVTKSSVHHISLQASSHKSTISKQEEIQKEIHDDQSIPEEPDNSSLSLAEKMALFNKLSQPITKEVSTRSRMDIRQRRMNARYQTQPVTLVEVEQYKNGGEKIGPLSHSVVTSVATLTSRPSPVSPGDMHTAKSSSYEERISSVSSTSALLHTSLETGSTSERSLLLSEPWSPPSVKDKVYEKEPRKPMLEETRVSAAEDSSSFGKCSSLHKEKTHPALDVAKEEEHVEEQKVPGHVASSSSRVELLSPQKLSQPAEHSESQAKPESALQVDAEDKKSLHPEPSMRRRKEIHEIVEPASELLGTSAAKTVSHTPAAAPAAVSYGPQASELSEEKPEEETAEISSTISNRSNEDLDTPGKAMSIKDRLALLKKSGEEDWKNRLNKKQEYSKVSVIERSTLTQLHEIEQSLTKKEPVYASVYSPALPAAHKCQYFVPIHQVSNATTLGQSSPVMFDERHPWRWKQRVTDGNGLHVSIEERKQQITVREEAWKTKGIGAANDSTQFTVAGRMVKKGLASPTAITPVASPMSLKFKTPVSKPLEEIEAKPDMNLESDRKLEKLESFLGKLHNRVGGLQDTTLSVTGQSVKEVMKVDDDETFGRFYRPFEFSTAHVPFDFEDDFDLLFSSEEPKLTSAVAEHKRAVRPARRVQASGNPLKMLAAREDILQEYTETRLNVAFMESKRMKVEKMSKNSNFSEVALAGLASKENFTSVSLRSVNMTEQHSNNSAVPYKKLMLVQIKGRRHVQSRLVEPRASSLNSGDCFLLITPHHCFLWVGEFANVIEKAKASELASIIQTKRELGCRASYVQTVEEGINTHTHGAKDFWKLLSGQTESQAAGSPEEDERYENAIIETNCIYRLLEDKLVPVDEFWGKVPKCSLLESKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGAFDYENCDINPLDPGECNSLIPRKGHGRPDWAIFGRLTEHNETILFKEKFLDWTEMKKPNEKGVCEDSPKLKEDPQSDVKPYDIMLMVPVPQKSVGTVLDGLNVARGYGLIEGDDCRQYEISTVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKYFVSTTVGQRQKTEHVRIAGKEKCAYFFWQGRNSPVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMIVHAGRREEEEENTQSDWRLYCVRGEVPVEGNLMEVACHCSSLRSRTSLILLSINKALIYLWHGCKVQIHAKEVGRTAANKIKEECPLEAGLHSSSKVTIYECDEGSEPPGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFTASEILYPSRHPNVVNSMPFLQEDLYTASQPALFLVDNHHEVYLWQGWWPSENIITGSARIRWDSDRKSAMETVLSYCKGKNIKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAQITEKDAEVSNQIILVEDVLAKLCKRIYPLADLLARPLPEGVDPLKLELYLSDKDFEVALEMTREEYNTLPPWKQVNTKKAKGLF

Target ORF information:

RefSeq Version	XM_012965659.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis supervillin (svil), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965659.1

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT AGCCAGACGT TTGGAAGGAA TTGATAACGA CCCACAACCA 
ATTATGCTAC AGACACGTTT ATTGGAAGAA GATATGCCAC GATATATGCG CGCAACAGAT 
GCATATAATC CACACATAGG TCATGCAAAT GAAGACACTG CCTCAGAGTC ATCAATAGAA 
ACACATTCTA GATGGCAAAA CCGCTGGCAG CCAAGCACTG AATCATCTTC TATGTCAGGT 
CAAATGGCGG TAGAGGAAAC AGAATCAAAA GCAGAAAGGA TAGCTCGATA CAAGGCGGAG 
CGAAGACGGC AATTGGCAGA AAGATATGGT GTCCCTATGG ATTCCGAAGC GGATTCCGAA 
TACCAGTCAA GGTACACGAG AATAAGGAAA GACGGTGAGA TCTCTGAAAA GAAATCTGTG 
TTAGAGGAAG ATGAAATTAA AGAGAATAGC TCAGATTATT TATACCGTCA AGACATTAAG 
GAGCCTCGCA GATTTCGAAT GTACGAATCA AAGCATCGGG ACATTAATGA GAATGTGTCG 
GAGAGAGATG CTCTCAAAAA TGTGGAAAAC AATAATAATC AATATCGAGA AATATCCGAG 
TTGGATGGAT CTACCTTTTC GTGCATGGGA CAAGCCATCA CAGTTGGGGA AGCACCCAGG 
TCTCCAAAGT TCCCTCGTCG TGCATCAGAG AAATCACTTC AGCTTTCCTC CTCTCCACCA 
TCTGAACAGC TACTGCTTAG CGACTCCAGA CAAGGAACAG GAAAACAGGC ACCAGAGTGG 
TCTCTCCAAA AAGATTCCGA TGGGGACACA GCGTCTCTCA TCAGCTGGCC GTCCAGAGTT 
AAAGTTAGAG AGAGGCTGGG GAGAGAACAT ATTCACGGAT TTCCCAAAAC TGACACAGAG 
ATCTCAACGC AAAGGAAACA TCCACCCGGG CCTGCCCACC CTGTTCCTTT CCAGTCGGAA 
ACCTCTGCTT TTCATAGGGT TTATAAACAG CCGGCATTAG TTCACCAGCC CATTCATGGC 
TACATCCAGC CTGCTAGTGT TGCTCATACA CCTAAAATTG TGGCACCTCA TGCACCTGAT 
TCGAGTGTCA TGGTGTCCGC TTTTACTACC TCCTTAAAAA ATCCCAGATC TTCCAGTGAT 
GTGTATATTG AGAAATCCCA CCATGCATTT CAAGGTCAGG ACAATATCGT AGAGCCATGC 
AAGCAGGTGA TCAGCAATGG CAAATCGCAC AGTATGTATG AAGAGAAGCC AACATATATT 
TCATCTGCCA GCAAGCCAAG AAACGAGACC TTGCCTGGGC ATAAAGACCC AGACCTGCAT 
GTTTCACACA GTTTACCACT TCAGAGACAA ACCTCTGGAA ACTTTGGGAA AAGCGAAAAG 
GTCTTGTTCC TTCAGAGTGG CAGATCCCAG GACCATACCA AGGAAGTATC TACGATGAAG 
CATAAAATGC TGACCCGTTC AATGTCTGAC ACCACCAACT CCCAAAAGAT GGAAGCTTTT 
AAGATAAAGG ACTCCGATGT CCAGAAAGAG ATGGTGTTTT TGCCTTCTGA AGACCATGTT 
GCCATTCCAA ATGGAGAAGC CGGCATTGTC AATACCAAAG TGTCAGTTGC TCAGCTTAGG 
AGTGCATATT TGGAGACGGC CAGCCACGCA CAAAAATCTG AGTTTGAACC TAAGGCTGAA 
ATGCCAACGT CAGGTGCTTT GTCTGCTACT ACTGAGCGGG AAAGAGGCCC TCGAAGGCCG 
AGACTTTATT TCTCTCCTGG TGAAAGTAGA AAGACCTCTG AAAGATTTAG AACCCAGCCT 
GTAACGTCAG CTGAGCGAAA TCAGATGGAT AGATCAAGCT TCCAGTCACC AGAGCAGATT 
GCTGAAGAAG AAGCAAAAGT GGATGAAAGA GCCAAACTTA GCGTGGCTGC GAAAAGGCTT 
CTATTCAGGG AAATGGAAAA ATCTCTGGAG TCGAAACCAG CCAAGCCACG TGCCAGAAAT 
GCAGCAGTGG AGAGGAGGCT TCGCCGAGCA CAAGACAGGT CTCGGACGCA ACCCGTTACC 
AGTGAAGAGG TGGTCATTGC TGCTACTGAG CCTAGTGCTG CTTCGCACAC TGTGACCACA 
CACACGGCCC TCCCTCGAAT ACCTAGCCCC ACTGTAACTA AGAGCTCTGT ACACCATATC 
AGTTTGCAGG CATCATCCCA TAAAAGCACA ATATCAAAGC AAGAGGAGAT ACAAAAGGAA 
ATACACGATG ACCAGAGCAT TCCCGAAGAG CCTGATAATT CCTCGCTTTC CTTGGCTGAA 
AAAATGGCAT TATTTAATAA GCTTTCCCAG CCAATAACAA AAGAGGTGTC TACGAGGAGC 
AGAATGGATA TTCGACAAAG GCGAATGAAT GCGCGTTACC AGACTCAGCC CGTCACCCTT 
GTGGAGGTTG AACAGTATAA GAATGGAGGC GAAAAGATCG GCCCCTTATC TCACAGTGTT 
GTAACTTCCG TAGCCACCCT AACCTCTCGG CCGTCTCCAG TTAGCCCTGG GGATATGCAT 
ACTGCAAAGT CCTCCAGTTA TGAAGAGAGA ATATCAAGTG TCTCCAGCAC TTCAGCCCTG 
CTGCACACTT CACTGGAAAC AGGAAGCACT TCGGAAAGGA GCTTGCTGCT CTCGGAGCCC 
TGGAGTCCCC CAAGTGTGAA AGATAAAGTA TATGAAAAGG AACCGAGGAA ACCAATGTTG 
GAAGAGACAA GGGTCTCTGC AGCAGAAGAT AGCAGCAGCT TCGGAAAATG CAGCTCGCTC 
CATAAGGAAA AAACCCACCC CGCGCTTGAT GTGGCAAAGG AGGAAGAACA TGTTGAGGAG 
CAGAAAGTGC CTGGGCATGT TGCCTCAAGT AGCAGCAGAG TGGAGTTGCT CAGCCCCCAG 
AAGCTGTCCC AGCCTGCTGA GCATTCAGAA TCCCAAGCAA AGCCTGAAAG TGCCCTGCAG 
GTAGACGCTG AAGATAAGAA GAGCCTACAT CCCGAGCCCA GCATGAGAAG AAGGAAAGAG 
ATCCACGAGA TTGTTGAGCC GGCCTCTGAA CTCCTTGGCA CCAGCGCTGC AAAGACCGTT 
TCTCACACTC CAGCTGCTGC TCCTGCTGCG GTGTCCTACG GACCACAGGC ATCAGAGCTA 
AGTGAGGAAA AGCCCGAGGA GGAAACAGCT GAGATCTCCT CCACCATCAG CAACAGGTCC 
AACGAGGATC TCGATACGCC CGGCAAAGCC ATGTCCATCA AAGACAGGCT GGCGCTGCTA 
AAGAAGAGTG GGGAAGAGGA TTGGAAAAAC AGGCTTAACA AAAAGCAGGA GTACAGCAAA 
GTGTCTGTCA TCGAGCGCAG CACGCTCACC CAGCTCCATG AGATTGAGCA GTCGCTGACA 
AAGAAGGAAC CTGTCTATGC TTCTGTTTAT TCTCCTGCTC TACCTGCCGC CCATAAATGC 
CAGTATTTTG TTCCTATACA TCAGGTCAGT AATGCTACGA CCCTGGGGCA GTCCAGCCCT 
GTGATGTTCG ATGAGCGGCA CCCCTGGAGA TGGAAGCAAA GGGTGACTGA CGGCAATGGG 
CTTCACGTGT CTATTGAAGA AAGGAAGCAG CAGATTACAG TCAGGGAGGA GGCCTGGAAA 
ACCAAAGGCA TAGGGGCAGC AAATGATTCC ACACAATTTA CAGTGGCCGG CCGCATGGTT 
AAGAAAGGTC TGGCCTCACC TACAGCCATT ACCCCCGTCG CATCTCCAAT GTCTCTCAAA 
TTTAAAACTC CAGTTTCCAA GCCTCTGGAA GAAATTGAAG CCAAACCTGA TATGAATTTG 
GAATCTGACA GGAAGTTAGA AAAACTAGAA TCATTTTTGG GAAAACTGCA CAACAGGGTT 
GGAGGGCTAC AAGATACCAC ACTGTCAGTC ACAGGCCAAT CAGTGAAGGA GGTGATGAAG 
GTGGACGATG ATGAAACTTT CGGAAGGTTC TACCGCCCTT TTGAATTCTC CACTGCCCAC 
GTGCCATTTG ATTTTGAAGA TGATTTTGAT CTTCTCTTCA GTTCGGAGGA ACCAAAGCTG 
ACATCTGCTG TAGCAGAACA CAAGCGTGCC GTCCGGCCAG CCCGCCGGGT GCAAGCGTCG 
GGAAACCCAC TGAAGATGCT TGCGGCCAGG GAGGACATCC TACAGGAGTA CACAGAGACG 
CGGCTGAACG TGGCGTTCAT GGAGTCCAAG AGAATGAAAG TAGAAAAAAT GTCCAAGAAC 
TCAAACTTCT CAGAAGTCGC ACTTGCAGGC CTGGCAAGCA AAGAAAACTT TACCAGCGTA 
AGCCTGCGGA GTGTCAATAT GACCGAGCAG CACTCCAACA ACAGCGCCGT GCCATACAAG 
AAGCTTATGC TAGTTCAGAT TAAAGGACGC AGGCACGTTC AGTCGAGGCT GGTGGAACCG 
CGTGCATCCT CTCTGAATAG CGGAGACTGC TTCCTGCTGA TCACCCCGCA CCACTGCTTC 
TTGTGGGTCG GAGAGTTTGC AAACGTCATT GAGAAAGCCA AGGCTTCTGA GCTGGCATCA 
ATAATTCAGA CCAAGAGGGA ACTTGGATGC AGGGCATCGT ATGTGCAAAC AGTCGAGGAA 
GGAATAAATA CCCACACGCA TGGAGCCAAG GATTTTTGGA AACTTCTGAG CGGTCAGACT 
GAATCTCAAG CTGCTGGGAG CCCAGAGGAG GATGAGAGAT ATGAAAATGC CATCATAGAA 
ACAAACTGTA TTTATCGATT GCTGGAAGAT AAGCTGGTCC CTGTTGATGA ATTTTGGGGC 
AAAGTACCTA AGTGTTCACT TTTAGAGTCT AAAGAGGTGC TTGTGTTTGA TTTTGGAAGT 
GAAGTGTATG TGTGGCATGG GAAGGAGGTG ACCTTGGCAC AGAGGAAGGT GGCGTTCCAG 
TTAGCCAAAC ATCTCTGGAA TGGAGCTTTT GACTATGAAA ATTGTGATAT CAATCCTCTG 
GACCCTGGGG AATGCAATTC TCTCATTCCA AGGAAAGGGC ACGGGCGCCC TGATTGGGCA 
ATATTTGGAA GGCTGACAGA GCATAATGAA ACCATACTGT TCAAGGAAAA GTTCCTGGAT 
TGGACAGAAA TGAAGAAACC CAATGAGAAG GGTGTGTGCG AGGACTCACC GAAGCTTAAG 
GAAGACCCAC AATCTGATGT GAAGCCTTAT GACATCATGC TGATGGTCCC AGTTCCCCAG 
AAATCTGTGG GTACGGTTCT TGATGGATTA AATGTCGCCC GTGGTTATGG ACTTATTGAG 
GGAGATGATT GCCGCCAGTA TGAAATCTCA ACCGTCTCGG TGGATGTCTG GCATATCCTT 
GAATTCGACT ACAGCCGGCT GCCGAAGCAG AGCATTGGCC AGTTCCACGA GGGAGACGCC 
TATGTGGTAA AATGGAAGTA CTTTGTGAGC ACAACAGTTG GACAGCGCCA GAAAACCGAG 
CACGTCCGGA TAGCGGGTAA AGAGAAGTGC GCCTACTTCT TCTGGCAGGG ACGGAACTCG 
CCCGTAAGTG AGAAGGGAAC CTCAGCGCTG ATGACTGTGG AGCTTGATGA AGAGCGGGGA 
GCACAGGTCC AGGTGCTTCA GGGAAAAGAG CCCCCCTGTT TCTTACAATG TTTCCAGGGA 
GGAATGATTG TGCATGCTGG GAGACGGGAA GAAGAGGAAG AGAACACGCA AAGTGACTGG 
CGGTTGTACT GTGTACGTGG GGAGGTGCCG GTGGAAGGGA ATTTGATGGA AGTCGCTTGT 
CACTGCAGCA GCCTGCGCTC CAGGACCTCC TTGATACTCC TCAGTATAAA TAAAGCGCTC 
ATCTACCTTT GGCACGGCTG CAAAGTCCAA ATCCATGCCA AGGAAGTGGG ACGGACGGCA 
GCCAATAAAA TAAAAGAGGA GTGCCCCCTG GAAGCCGGGC TACACAGCAG CAGTAAGGTG 
ACCATATATG AATGTGATGA AGGCTCGGAA CCCCCCGGAT TCTGGGATGC GCTGGGCAGG 
CGAGACCGTA AAGCGTACGA CTGCATGTTG CAAGATCCTG GGAAATTTAA CTTCACGCCA 
CGACTGTTCA GCCTCAGTAG TTCATCGGGA GAGTTTACAG CATCAGAAAT CCTGTATCCT 
TCAAGGCATC CGAATGTGGT TAACTCCATG CCATTTTTAC AAGAAGACCT GTATACGGCC 
TCCCAGCCAG CTTTATTCCT CGTTGACAAC CATCACGAGG TTTACCTGTG GCAAGGTTGG 
TGGCCGTCTG AGAATATTAT TACTGGGTCT GCTCGTATTC GGTGGGATTC AGACAGAAAA 
AGCGCAATGG AGACCGTACT CAGCTACTGC AAAGGAAAGA ATATCAAGAA ACCTCCCAAG 
TCGTATCTAA TCCATGCCGG GCTGGAGCCT CTGACCTTTA CCAACATGTT TCCAAGCTGG 
GAACATAGGG AAGACATTGC CCAAATCACA GAAAAGGATG CGGAAGTCTC TAACCAGATA 
ATCCTTGTAG AAGATGTACT CGCTAAACTT TGTAAAAGAA TCTACCCCTT GGCAGATCTT 
CTTGCCCGCC CGTTGCCCGA AGGAGTCGAC CCTCTCAAGC TTGAGCTCTA TCTGTCAGAT 
AAAGACTTTG AGGTTGCGTT AGAAATGACA AGAGAAGAAT ACAACACACT GCCGCCCTGG 
AAACAGGTTA ATACAAAGAA AGCCAAAGGA CTTTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

svil ( XM_012965655.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821109.1 Xenopus tropicalis supervillin (svil), transcript variant X3, mRNA.

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CloneID	OXa29954
Clone ID Related Accession (Same CDS sequence)	XM_012965659.1 , XM_012965655.2
Accession Version	XM_012965655.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	supervillin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965655.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT AGCCAGACGT TTGGAAGGAA TTGATAACGA CCCACAACCA 
ATTATGCTAC AGACACGTTT ATTGGAAGAA GATATGCCAC GATATATGCG CGCAACAGAT 
GCATATAATC CACACATAGG TCATGCAAAT GAAGACACTG CCTCAGAGTC ATCAATAGAA 
ACACATTCTA GATGGCAAAA CCGCTGGCAG CCAAGCACTG AATCATCTTC TATGTCAGGT 
CAAATGGCGG TAGAGGAAAC AGAATCAAAA GCAGAAAGGA TAGCTCGATA CAAGGCGGAG 
CGAAGACGGC AATTGGCAGA AAGATATGGT GTCCCTATGG ATTCCGAAGC GGATTCCGAA 
TACCAGTCAA GGTACACGAG AATAAGGAAA GACGGTGAGA TCTCTGAAAA GAAATCTGTG 
TTAGAGGAAG ATGAAATTAA AGAGAATAGC TCAGATTATT TATACCGTCA AGACATTAAG 
GAGCCTCGCA GATTTCGAAT GTACGAATCA AAGCATCGGG ACATTAATGA GAATGTGTCG 
GAGAGAGATG CTCTCAAAAA TGTGGAAAAC AATAATAATC AATATCGAGA AATATCCGAG 
TTGGATGGAT CTACCTTTTC GTGCATGGGA CAAGCCATCA CAGTTGGGGA AGCACCCAGG 
TCTCCAAAGT TCCCTCGTCG TGCATCAGAG AAATCACTTC AGCTTTCCTC CTCTCCACCA 
TCTGAACAGC TACTGCTTAG CGACTCCAGA CAAGGAACAG GAAAACAGGC ACCAGAGTGG 
TCTCTCCAAA AAGATTCCGA TGGGGACACA GCGTCTCTCA TCAGCTGGCC GTCCAGAGTT 
AAAGTTAGAG AGAGGCTGGG GAGAGAACAT ATTCACGGAT TTCCCAAAAC TGACACAGAG 
ATCTCAACGC AAAGGAAACA TCCACCCGGG CCTGCCCACC CTGTTCCTTT CCAGTCGGAA 
ACCTCTGCTT TTCATAGGGT TTATAAACAG CCGGCATTAG TTCACCAGCC CATTCATGGC 
TACATCCAGC CTGCTAGTGT TGCTCATACA CCTAAAATTG TGGCACCTCA TGCACCTGAT 
TCGAGTGTCA TGGTGTCCGC TTTTACTACC TCCTTAAAAA ATCCCAGATC TTCCAGTGAT 
GTGTATATTG AGAAATCCCA CCATGCATTT CAAGGTCAGG ACAATATCGT AGAGCCATGC 
AAGCAGGTGA TCAGCAATGG CAAATCGCAC AGTATGTATG AAGAGAAGCC AACATATATT 
TCATCTGCCA GCAAGCCAAG AAACGAGACC TTGCCTGGGC ATAAAGACCC AGACCTGCAT 
GTTTCACACA GTTTACCACT TCAGAGACAA ACCTCTGGAA ACTTTGGGAA AAGCGAAAAG 
GTCTTGTTCC TTCAGAGTGG CAGATCCCAG GACCATACCA AGGAAGTATC TACGATGAAG 
CATAAAATGC TGACCCGTTC AATGTCTGAC ACCACCAACT CCCAAAAGAT GGAAGCTTTT 
AAGATAAAGG ACTCCGATGT CCAGAAAGAG ATGGTGTTTT TGCCTTCTGA AGACCATGTT 
GCCATTCCAA ATGGAGAAGC CGGCATTGTC AATACCAAAG TGTCAGTTGC TCAGCTTAGG 
AGTGCATATT TGGAGACGGC CAGCCACGCA CAAAAATCTG AGTTTGAACC TAAGGCTGAA 
ATGCCAACGT CAGGTGCTTT GTCTGCTACT ACTGAGCGGG AAAGAGGCCC TCGAAGGCCG 
AGACTTTATT TCTCTCCTGG TGAAAGTAGA AAGACCTCTG AAAGATTTAG AACCCAGCCT 
GTAACGTCAG CTGAGCGAAA TCAGATGGAT AGATCAAGCT TCCAGTCACC AGAGCAGATT 
GCTGAAGAAG AAGCAAAAGT GGATGAAAGA GCCAAACTTA GCGTGGCTGC GAAAAGGCTT 
CTATTCAGGG AAATGGAAAA ATCTCTGGAG TCGAAACCAG CCAAGCCACG TGCCAGAAAT 
GCAGCAGTGG AGAGGAGGCT TCGCCGAGCA CAAGACAGGT CTCGGACGCA ACCCGTTACC 
AGTGAAGAGG TGGTCATTGC TGCTACTGAG CCTAGTGCTG CTTCGCACAC TGTGACCACA 
CACACGGCCC TCCCTCGAAT ACCTAGCCCC ACTGTAACTA AGAGCTCTGT ACACCATATC 
AGTTTGCAGG CATCATCCCA TAAAAGCACA ATATCAAAGC AAGAGGAGAT ACAAAAGGAA 
ATACACGATG ACCAGAGCAT TCCCGAAGAG CCTGATAATT CCTCGCTTTC CTTGGCTGAA 
AAAATGGCAT TATTTAATAA GCTTTCCCAG CCAATAACAA AAGAGGTGTC TACGAGGAGC 
AGAATGGATA TTCGACAAAG GCGAATGAAT GCGCGTTACC AGACTCAGCC CGTCACCCTT 
GTGGAGGTTG AACAGTATAA GAATGGAGGC GAAAAGATCG GCCCCTTATC TCACAGTGTT 
GTAACTTCCG TAGCCACCCT AACCTCTCGG CCGTCTCCAG TTAGCCCTGG GGATATGCAT 
ACTGCAAAGT CCTCCAGTTA TGAAGAGAGA ATATCAAGTG TCTCCAGCAC TTCAGCCCTG 
CTGCACACTT CACTGGAAAC AGGAAGCACT TCGGAAAGGA GCTTGCTGCT CTCGGAGCCC 
TGGAGTCCCC CAAGTGTGAA AGATAAAGTA TATGAAAAGG AACCGAGGAA ACCAATGTTG 
GAAGAGACAA GGGTCTCTGC AGCAGAAGAT AGCAGCAGCT TCGGAAAATG CAGCTCGCTC 
CATAAGGAAA AAACCCACCC CGCGCTTGAT GTGGCAAAGG AGGAAGAACA TGTTGAGGAG 
CAGAAAGTGC CTGGGCATGT TGCCTCAAGT AGCAGCAGAG TGGAGTTGCT CAGCCCCCAG 
AAGCTGTCCC AGCCTGCTGA GCATTCAGAA TCCCAAGCAA AGCCTGAAAG TGCCCTGCAG 
GTAGACGCTG AAGATAAGAA GAGCCTACAT CCCGAGCCCA GCATGAGAAG AAGGAAAGAG 
ATCCACGAGA TTGTTGAGCC GGCCTCTGAA CTCCTTGGCA CCAGCGCTGC AAAGACCGTT 
TCTCACACTC CAGCTGCTGC TCCTGCTGCG GTGTCCTACG GACCACAGGC ATCAGAGCTA 
AGTGAGGAAA AGCCCGAGGA GGAAACAGCT GAGATCTCCT CCACCATCAG CAACAGGTCC 
AACGAGGATC TCGATACGCC CGGCAAAGCC ATGTCCATCA AAGACAGGCT GGCGCTGCTA 
AAGAAGAGTG GGGAAGAGGA TTGGAAAAAC AGGCTTAACA AAAAGCAGGA GTACAGCAAA 
GTGTCTGTCA TCGAGCGCAG CACGCTCACC CAGCTCCATG AGATTGAGCA GTCGCTGACA 
AAGAAGGAAC CTGTCTATGC TTCTGTTTAT TCTCCTGCTC TACCTGCCGC CCATAAATGC 
CAGTATTTTG TTCCTATACA TCAGGTCAGT AATGCTACGA CCCTGGGGCA GTCCAGCCCT 
GTGATGTTCG ATGAGCGGCA CCCCTGGAGA TGGAAGCAAA GGGTGACTGA CGGCAATGGG 
CTTCACGTGT CTATTGAAGA AAGGAAGCAG CAGATTACAG TCAGGGAGGA GGCCTGGAAA 
ACCAAAGGCA TAGGGGCAGC AAATGATTCC ACACAATTTA CAGTGGCCGG CCGCATGGTT 
AAGAAAGGTC TGGCCTCACC TACAGCCATT ACCCCCGTCG CATCTCCAAT GTCTCTCAAA 
TTTAAAACTC CAGTTTCCAA GCCTCTGGAA GAAATTGAAG CCAAACCTGA TATGAATTTG 
GAATCTGACA GGAAGTTAGA AAAACTAGAA TCATTTTTGG GAAAACTGCA CAACAGGGTT 
GGAGGGCTAC AAGATACCAC ACTGTCAGTC ACAGGCCAAT CAGTGAAGGA GGTGATGAAG 
GTGGACGATG ATGAAACTTT CGGAAGGTTC TACCGCCCTT TTGAATTCTC CACTGCCCAC 
GTGCCATTTG ATTTTGAAGA TGATTTTGAT CTTCTCTTCA GTTCGGAGGA ACCAAAGCTG 
ACATCTGCTG TAGCAGAACA CAAGCGTGCC GTCCGGCCAG CCCGCCGGGT GCAAGCGTCG 
GGAAACCCAC TGAAGATGCT TGCGGCCAGG GAGGACATCC TACAGGAGTA CACAGAGACG 
CGGCTGAACG TGGCGTTCAT GGAGTCCAAG AGAATGAAAG TAGAAAAAAT GTCCAAGAAC 
TCAAACTTCT CAGAAGTCGC ACTTGCAGGC CTGGCAAGCA AAGAAAACTT TACCAGCGTA 
AGCCTGCGGA GTGTCAATAT GACCGAGCAG CACTCCAACA ACAGCGCCGT GCCATACAAG 
AAGCTTATGC TAGTTCAGAT TAAAGGACGC AGGCACGTTC AGTCGAGGCT GGTGGAACCG 
CGTGCATCCT CTCTGAATAG CGGAGACTGC TTCCTGCTGA TCACCCCGCA CCACTGCTTC 
TTGTGGGTCG GAGAGTTTGC AAACGTCATT GAGAAAGCCA AGGCTTCTGA GCTGGCATCA 
ATAATTCAGA CCAAGAGGGA ACTTGGATGC AGGGCATCGT ATGTGCAAAC AGTCGAGGAA 
GGAATAAATA CCCACACGCA TGGAGCCAAG GATTTTTGGA AACTTCTGAG CGGTCAGACT 
GAATCTCAAG CTGCTGGGAG CCCAGAGGAG GATGAGAGAT ATGAAAATGC CATCATAGAA 
ACAAACTGTA TTTATCGATT GCTGGAAGAT AAGCTGGTCC CTGTTGATGA ATTTTGGGGC 
AAAGTACCTA AGTGTTCACT TTTAGAGTCT AAAGAGGTGC TTGTGTTTGA TTTTGGAAGT 
GAAGTGTATG TGTGGCATGG GAAGGAGGTG ACCTTGGCAC AGAGGAAGGT GGCGTTCCAG 
TTAGCCAAAC ATCTCTGGAA TGGAGCTTTT GACTATGAAA ATTGTGATAT CAATCCTCTG 
GACCCTGGGG AATGCAATTC TCTCATTCCA AGGAAAGGGC ACGGGCGCCC TGATTGGGCA 
ATATTTGGAA GGCTGACAGA GCATAATGAA ACCATACTGT TCAAGGAAAA GTTCCTGGAT 
TGGACAGAAA TGAAGAAACC CAATGAGAAG GGTGTGTGCG AGGACTCACC GAAGCTTAAG 
GAAGACCCAC AATCTGATGT GAAGCCTTAT GACATCATGC TGATGGTCCC AGTTCCCCAG 
AAATCTGTGG GTACGGTTCT TGATGGATTA AATGTCGCCC GTGGTTATGG ACTTATTGAG 
GGAGATGATT GCCGCCAGTA TGAAATCTCA ACCGTCTCGG TGGATGTCTG GCATATCCTT 
GAATTCGACT ACAGCCGGCT GCCGAAGCAG AGCATTGGCC AGTTCCACGA GGGAGACGCC 
TATGTGGTAA AATGGAAGTA CTTTGTGAGC ACAACAGTTG GACAGCGCCA GAAAACCGAG 
CACGTCCGGA TAGCGGGTAA AGAGAAGTGC GCCTACTTCT TCTGGCAGGG ACGGAACTCG 
CCCGTAAGTG AGAAGGGAAC CTCAGCGCTG ATGACTGTGG AGCTTGATGA AGAGCGGGGA 
GCACAGGTCC AGGTGCTTCA GGGAAAAGAG CCCCCCTGTT TCTTACAATG TTTCCAGGGA 
GGAATGATTG TGCATGCTGG GAGACGGGAA GAAGAGGAAG AGAACACGCA AAGTGACTGG 
CGGTTGTACT GTGTACGTGG GGAGGTGCCG GTGGAAGGGA ATTTGATGGA AGTCGCTTGT 
CACTGCAGCA GCCTGCGCTC CAGGACCTCC TTGATACTCC TCAGTATAAA TAAAGCGCTC 
ATCTACCTTT GGCACGGCTG CAAAGTCCAA ATCCATGCCA AGGAAGTGGG ACGGACGGCA 
GCCAATAAAA TAAAAGAGGA GTGCCCCCTG GAAGCCGGGC TACACAGCAG CAGTAAGGTG 
ACCATATATG AATGTGATGA AGGCTCGGAA CCCCCCGGAT TCTGGGATGC GCTGGGCAGG 
CGAGACCGTA AAGCGTACGA CTGCATGTTG CAAGATCCTG GGAAATTTAA CTTCACGCCA 
CGACTGTTCA GCCTCAGTAG TTCATCGGGA GAGTTTACAG CATCAGAAAT CCTGTATCCT 
TCAAGGCATC CGAATGTGGT TAACTCCATG CCATTTTTAC AAGAAGACCT GTATACGGCC 
TCCCAGCCAG CTTTATTCCT CGTTGACAAC CATCACGAGG TTTACCTGTG GCAAGGTTGG 
TGGCCGTCTG AGAATATTAT TACTGGGTCT GCTCGTATTC GGTGGGATTC AGACAGAAAA 
AGCGCAATGG AGACCGTACT CAGCTACTGC AAAGGAAAGA ATATCAAGAA ACCTCCCAAG 
TCGTATCTAA TCCATGCCGG GCTGGAGCCT CTGACCTTTA CCAACATGTT TCCAAGCTGG 
GAACATAGGG AAGACATTGC CCAAATCACA GAAAAGGATG CGGAAGTCTC TAACCAGATA 
ATCCTTGTAG AAGATGTACT CGCTAAACTT TGTAAAAGAA TCTACCCCTT GGCAGATCTT 
CTTGCCCGCC CGTTGCCCGA AGGAGTCGAC CCTCTCAAGC TTGAGCTCTA TCTGTCAGAT 
AAAGACTTTG AGGTTGCGTT AGAAATGACA AGAGAAGAAT ACAACACACT GCCGCCCTGG 
AAACAGGTTA ATACAAAGAA AGCCAAAGGA CTTTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821109.1
CDS	565..7203
Translation	MKRKERIARRLEGIDNDPQPIMLQTRLLEEDMPRYMRATDAYNPHIGHANEDTASESSIETHSRWQNRWQPSTESSSMSGQMAVEETESKAERIARYKAERRRQLAERYGVPMDSEADSEYQSRYTRIRKDGEISEKKSVLEEDEIKENSSDYLYRQDIKEPRRFRMYESKHRDINENVSERDALKNVENNNNQYREISELDGSTFSCMGQAITVGEAPRSPKFPRRASEKSLQLSSSPPSEQLLLSDSRQGTGKQAPEWSLQKDSDGDTASLISWPSRVKVRERLGREHIHGFPKTDTEISTQRKHPPGPAHPVPFQSETSAFHRVYKQPALVHQPIHGYIQPASVAHTPKIVAPHAPDSSVMVSAFTTSLKNPRSSSDVYIEKSHHAFQGQDNIVEPCKQVISNGKSHSMYEEKPTYISSASKPRNETLPGHKDPDLHVSHSLPLQRQTSGNFGKSEKVLFLQSGRSQDHTKEVSTMKHKMLTRSMSDTTNSQKMEAFKIKDSDVQKEMVFLPSEDHVAIPNGEAGIVNTKVSVAQLRSAYLETASHAQKSEFEPKAEMPTSGALSATTERERGPRRPRLYFSPGESRKTSERFRTQPVTSAERNQMDRSSFQSPEQIAEEEAKVDERAKLSVAAKRLLFREMEKSLESKPAKPRARNAAVERRLRRAQDRSRTQPVTSEEVVIAATEPSAASHTVTTHTALPRIPSPTVTKSSVHHISLQASSHKSTISKQEEIQKEIHDDQSIPEEPDNSSLSLAEKMALFNKLSQPITKEVSTRSRMDIRQRRMNARYQTQPVTLVEVEQYKNGGEKIGPLSHSVVTSVATLTSRPSPVSPGDMHTAKSSSYEERISSVSSTSALLHTSLETGSTSERSLLLSEPWSPPSVKDKVYEKEPRKPMLEETRVSAAEDSSSFGKCSSLHKEKTHPALDVAKEEEHVEEQKVPGHVASSSSRVELLSPQKLSQPAEHSESQAKPESALQVDAEDKKSLHPEPSMRRRKEIHEIVEPASELLGTSAAKTVSHTPAAAPAAVSYGPQASELSEEKPEEETAEISSTISNRSNEDLDTPGKAMSIKDRLALLKKSGEEDWKNRLNKKQEYSKVSVIERSTLTQLHEIEQSLTKKEPVYASVYSPALPAAHKCQYFVPIHQVSNATTLGQSSPVMFDERHPWRWKQRVTDGNGLHVSIEERKQQITVREEAWKTKGIGAANDSTQFTVAGRMVKKGLASPTAITPVASPMSLKFKTPVSKPLEEIEAKPDMNLESDRKLEKLESFLGKLHNRVGGLQDTTLSVTGQSVKEVMKVDDDETFGRFYRPFEFSTAHVPFDFEDDFDLLFSSEEPKLTSAVAEHKRAVRPARRVQASGNPLKMLAAREDILQEYTETRLNVAFMESKRMKVEKMSKNSNFSEVALAGLASKENFTSVSLRSVNMTEQHSNNSAVPYKKLMLVQIKGRRHVQSRLVEPRASSLNSGDCFLLITPHHCFLWVGEFANVIEKAKASELASIIQTKRELGCRASYVQTVEEGINTHTHGAKDFWKLLSGQTESQAAGSPEEDERYENAIIETNCIYRLLEDKLVPVDEFWGKVPKCSLLESKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGAFDYENCDINPLDPGECNSLIPRKGHGRPDWAIFGRLTEHNETILFKEKFLDWTEMKKPNEKGVCEDSPKLKEDPQSDVKPYDIMLMVPVPQKSVGTVLDGLNVARGYGLIEGDDCRQYEISTVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKYFVSTTVGQRQKTEHVRIAGKEKCAYFFWQGRNSPVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMIVHAGRREEEEENTQSDWRLYCVRGEVPVEGNLMEVACHCSSLRSRTSLILLSINKALIYLWHGCKVQIHAKEVGRTAANKIKEECPLEAGLHSSSKVTIYECDEGSEPPGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFTASEILYPSRHPNVVNSMPFLQEDLYTASQPALFLVDNHHEVYLWQGWWPSENIITGSARIRWDSDRKSAMETVLSYCKGKNIKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAQITEKDAEVSNQIILVEDVLAKLCKRIYPLADLLARPLPEGVDPLKLELYLSDKDFEVALEMTREEYNTLPPWKQVNTKKAKGLF

Target ORF information:

RefSeq Version	XM_012965655.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis supervillin (svil), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965655.2

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT AGCCAGACGT TTGGAAGGAA TTGATAACGA CCCACAACCA 
ATTATGCTAC AGACACGTTT ATTGGAAGAA GATATGCCAC GATATATGCG CGCAACAGAT 
GCATATAATC CACACATAGG TCATGCAAAT GAAGACACTG CCTCAGAGTC ATCAATAGAA 
ACACATTCTA GATGGCAAAA CCGCTGGCAG CCAAGCACTG AATCATCTTC TATGTCAGGT 
CAAATGGCGG TAGAGGAAAC AGAATCAAAA GCAGAAAGGA TAGCTCGATA CAAGGCGGAG 
CGAAGACGGC AATTGGCAGA AAGATATGGT GTCCCTATGG ATTCCGAAGC GGATTCCGAA 
TACCAGTCAA GGTACACGAG AATAAGGAAA GACGGTGAGA TCTCTGAAAA GAAATCTGTG 
TTAGAGGAAG ATGAAATTAA AGAGAATAGC TCAGATTATT TATACCGTCA AGACATTAAG 
GAGCCTCGCA GATTTCGAAT GTACGAATCA AAGCATCGGG ACATTAATGA GAATGTGTCG 
GAGAGAGATG CTCTCAAAAA TGTGGAAAAC AATAATAATC AATATCGAGA AATATCCGAG 
TTGGATGGAT CTACCTTTTC GTGCATGGGA CAAGCCATCA CAGTTGGGGA AGCACCCAGG 
TCTCCAAAGT TCCCTCGTCG TGCATCAGAG AAATCACTTC AGCTTTCCTC CTCTCCACCA 
TCTGAACAGC TACTGCTTAG CGACTCCAGA CAAGGAACAG GAAAACAGGC ACCAGAGTGG 
TCTCTCCAAA AAGATTCCGA TGGGGACACA GCGTCTCTCA TCAGCTGGCC GTCCAGAGTT 
AAAGTTAGAG AGAGGCTGGG GAGAGAACAT ATTCACGGAT TTCCCAAAAC TGACACAGAG 
ATCTCAACGC AAAGGAAACA TCCACCCGGG CCTGCCCACC CTGTTCCTTT CCAGTCGGAA 
ACCTCTGCTT TTCATAGGGT TTATAAACAG CCGGCATTAG TTCACCAGCC CATTCATGGC 
TACATCCAGC CTGCTAGTGT TGCTCATACA CCTAAAATTG TGGCACCTCA TGCACCTGAT 
TCGAGTGTCA TGGTGTCCGC TTTTACTACC TCCTTAAAAA ATCCCAGATC TTCCAGTGAT 
GTGTATATTG AGAAATCCCA CCATGCATTT CAAGGTCAGG ACAATATCGT AGAGCCATGC 
AAGCAGGTGA TCAGCAATGG CAAATCGCAC AGTATGTATG AAGAGAAGCC AACATATATT 
TCATCTGCCA GCAAGCCAAG AAACGAGACC TTGCCTGGGC ATAAAGACCC AGACCTGCAT 
GTTTCACACA GTTTACCACT TCAGAGACAA ACCTCTGGAA ACTTTGGGAA AAGCGAAAAG 
GTCTTGTTCC TTCAGAGTGG CAGATCCCAG GACCATACCA AGGAAGTATC TACGATGAAG 
CATAAAATGC TGACCCGTTC AATGTCTGAC ACCACCAACT CCCAAAAGAT GGAAGCTTTT 
AAGATAAAGG ACTCCGATGT CCAGAAAGAG ATGGTGTTTT TGCCTTCTGA AGACCATGTT 
GCCATTCCAA ATGGAGAAGC CGGCATTGTC AATACCAAAG TGTCAGTTGC TCAGCTTAGG 
AGTGCATATT TGGAGACGGC CAGCCACGCA CAAAAATCTG AGTTTGAACC TAAGGCTGAA 
ATGCCAACGT CAGGTGCTTT GTCTGCTACT ACTGAGCGGG AAAGAGGCCC TCGAAGGCCG 
AGACTTTATT TCTCTCCTGG TGAAAGTAGA AAGACCTCTG AAAGATTTAG AACCCAGCCT 
GTAACGTCAG CTGAGCGAAA TCAGATGGAT AGATCAAGCT TCCAGTCACC AGAGCAGATT 
GCTGAAGAAG AAGCAAAAGT GGATGAAAGA GCCAAACTTA GCGTGGCTGC GAAAAGGCTT 
CTATTCAGGG AAATGGAAAA ATCTCTGGAG TCGAAACCAG CCAAGCCACG TGCCAGAAAT 
GCAGCAGTGG AGAGGAGGCT TCGCCGAGCA CAAGACAGGT CTCGGACGCA ACCCGTTACC 
AGTGAAGAGG TGGTCATTGC TGCTACTGAG CCTAGTGCTG CTTCGCACAC TGTGACCACA 
CACACGGCCC TCCCTCGAAT ACCTAGCCCC ACTGTAACTA AGAGCTCTGT ACACCATATC 
AGTTTGCAGG CATCATCCCA TAAAAGCACA ATATCAAAGC AAGAGGAGAT ACAAAAGGAA 
ATACACGATG ACCAGAGCAT TCCCGAAGAG CCTGATAATT CCTCGCTTTC CTTGGCTGAA 
AAAATGGCAT TATTTAATAA GCTTTCCCAG CCAATAACAA AAGAGGTGTC TACGAGGAGC 
AGAATGGATA TTCGACAAAG GCGAATGAAT GCGCGTTACC AGACTCAGCC CGTCACCCTT 
GTGGAGGTTG AACAGTATAA GAATGGAGGC GAAAAGATCG GCCCCTTATC TCACAGTGTT 
GTAACTTCCG TAGCCACCCT AACCTCTCGG CCGTCTCCAG TTAGCCCTGG GGATATGCAT 
ACTGCAAAGT CCTCCAGTTA TGAAGAGAGA ATATCAAGTG TCTCCAGCAC TTCAGCCCTG 
CTGCACACTT CACTGGAAAC AGGAAGCACT TCGGAAAGGA GCTTGCTGCT CTCGGAGCCC 
TGGAGTCCCC CAAGTGTGAA AGATAAAGTA TATGAAAAGG AACCGAGGAA ACCAATGTTG 
GAAGAGACAA GGGTCTCTGC AGCAGAAGAT AGCAGCAGCT TCGGAAAATG CAGCTCGCTC 
CATAAGGAAA AAACCCACCC CGCGCTTGAT GTGGCAAAGG AGGAAGAACA TGTTGAGGAG 
CAGAAAGTGC CTGGGCATGT TGCCTCAAGT AGCAGCAGAG TGGAGTTGCT CAGCCCCCAG 
AAGCTGTCCC AGCCTGCTGA GCATTCAGAA TCCCAAGCAA AGCCTGAAAG TGCCCTGCAG 
GTAGACGCTG AAGATAAGAA GAGCCTACAT CCCGAGCCCA GCATGAGAAG AAGGAAAGAG 
ATCCACGAGA TTGTTGAGCC GGCCTCTGAA CTCCTTGGCA CCAGCGCTGC AAAGACCGTT 
TCTCACACTC CAGCTGCTGC TCCTGCTGCG GTGTCCTACG GACCACAGGC ATCAGAGCTA 
AGTGAGGAAA AGCCCGAGGA GGAAACAGCT GAGATCTCCT CCACCATCAG CAACAGGTCC 
AACGAGGATC TCGATACGCC CGGCAAAGCC ATGTCCATCA AAGACAGGCT GGCGCTGCTA 
AAGAAGAGTG GGGAAGAGGA TTGGAAAAAC AGGCTTAACA AAAAGCAGGA GTACAGCAAA 
GTGTCTGTCA TCGAGCGCAG CACGCTCACC CAGCTCCATG AGATTGAGCA GTCGCTGACA 
AAGAAGGAAC CTGTCTATGC TTCTGTTTAT TCTCCTGCTC TACCTGCCGC CCATAAATGC 
CAGTATTTTG TTCCTATACA TCAGGTCAGT AATGCTACGA CCCTGGGGCA GTCCAGCCCT 
GTGATGTTCG ATGAGCGGCA CCCCTGGAGA TGGAAGCAAA GGGTGACTGA CGGCAATGGG 
CTTCACGTGT CTATTGAAGA AAGGAAGCAG CAGATTACAG TCAGGGAGGA GGCCTGGAAA 
ACCAAAGGCA TAGGGGCAGC AAATGATTCC ACACAATTTA CAGTGGCCGG CCGCATGGTT 
AAGAAAGGTC TGGCCTCACC TACAGCCATT ACCCCCGTCG CATCTCCAAT GTCTCTCAAA 
TTTAAAACTC CAGTTTCCAA GCCTCTGGAA GAAATTGAAG CCAAACCTGA TATGAATTTG 
GAATCTGACA GGAAGTTAGA AAAACTAGAA TCATTTTTGG GAAAACTGCA CAACAGGGTT 
GGAGGGCTAC AAGATACCAC ACTGTCAGTC ACAGGCCAAT CAGTGAAGGA GGTGATGAAG 
GTGGACGATG ATGAAACTTT CGGAAGGTTC TACCGCCCTT TTGAATTCTC CACTGCCCAC 
GTGCCATTTG ATTTTGAAGA TGATTTTGAT CTTCTCTTCA GTTCGGAGGA ACCAAAGCTG 
ACATCTGCTG TAGCAGAACA CAAGCGTGCC GTCCGGCCAG CCCGCCGGGT GCAAGCGTCG 
GGAAACCCAC TGAAGATGCT TGCGGCCAGG GAGGACATCC TACAGGAGTA CACAGAGACG 
CGGCTGAACG TGGCGTTCAT GGAGTCCAAG AGAATGAAAG TAGAAAAAAT GTCCAAGAAC 
TCAAACTTCT CAGAAGTCGC ACTTGCAGGC CTGGCAAGCA AAGAAAACTT TACCAGCGTA 
AGCCTGCGGA GTGTCAATAT GACCGAGCAG CACTCCAACA ACAGCGCCGT GCCATACAAG 
AAGCTTATGC TAGTTCAGAT TAAAGGACGC AGGCACGTTC AGTCGAGGCT GGTGGAACCG 
CGTGCATCCT CTCTGAATAG CGGAGACTGC TTCCTGCTGA TCACCCCGCA CCACTGCTTC 
TTGTGGGTCG GAGAGTTTGC AAACGTCATT GAGAAAGCCA AGGCTTCTGA GCTGGCATCA 
ATAATTCAGA CCAAGAGGGA ACTTGGATGC AGGGCATCGT ATGTGCAAAC AGTCGAGGAA 
GGAATAAATA CCCACACGCA TGGAGCCAAG GATTTTTGGA AACTTCTGAG CGGTCAGACT 
GAATCTCAAG CTGCTGGGAG CCCAGAGGAG GATGAGAGAT ATGAAAATGC CATCATAGAA 
ACAAACTGTA TTTATCGATT GCTGGAAGAT AAGCTGGTCC CTGTTGATGA ATTTTGGGGC 
AAAGTACCTA AGTGTTCACT TTTAGAGTCT AAAGAGGTGC TTGTGTTTGA TTTTGGAAGT 
GAAGTGTATG TGTGGCATGG GAAGGAGGTG ACCTTGGCAC AGAGGAAGGT GGCGTTCCAG 
TTAGCCAAAC ATCTCTGGAA TGGAGCTTTT GACTATGAAA ATTGTGATAT CAATCCTCTG 
GACCCTGGGG AATGCAATTC TCTCATTCCA AGGAAAGGGC ACGGGCGCCC TGATTGGGCA 
ATATTTGGAA GGCTGACAGA GCATAATGAA ACCATACTGT TCAAGGAAAA GTTCCTGGAT 
TGGACAGAAA TGAAGAAACC CAATGAGAAG GGTGTGTGCG AGGACTCACC GAAGCTTAAG 
GAAGACCCAC AATCTGATGT GAAGCCTTAT GACATCATGC TGATGGTCCC AGTTCCCCAG 
AAATCTGTGG GTACGGTTCT TGATGGATTA AATGTCGCCC GTGGTTATGG ACTTATTGAG 
GGAGATGATT GCCGCCAGTA TGAAATCTCA ACCGTCTCGG TGGATGTCTG GCATATCCTT 
GAATTCGACT ACAGCCGGCT GCCGAAGCAG AGCATTGGCC AGTTCCACGA GGGAGACGCC 
TATGTGGTAA AATGGAAGTA CTTTGTGAGC ACAACAGTTG GACAGCGCCA GAAAACCGAG 
CACGTCCGGA TAGCGGGTAA AGAGAAGTGC GCCTACTTCT TCTGGCAGGG ACGGAACTCG 
CCCGTAAGTG AGAAGGGAAC CTCAGCGCTG ATGACTGTGG AGCTTGATGA AGAGCGGGGA 
GCACAGGTCC AGGTGCTTCA GGGAAAAGAG CCCCCCTGTT TCTTACAATG TTTCCAGGGA 
GGAATGATTG TGCATGCTGG GAGACGGGAA GAAGAGGAAG AGAACACGCA AAGTGACTGG 
CGGTTGTACT GTGTACGTGG GGAGGTGCCG GTGGAAGGGA ATTTGATGGA AGTCGCTTGT 
CACTGCAGCA GCCTGCGCTC CAGGACCTCC TTGATACTCC TCAGTATAAA TAAAGCGCTC 
ATCTACCTTT GGCACGGCTG CAAAGTCCAA ATCCATGCCA AGGAAGTGGG ACGGACGGCA 
GCCAATAAAA TAAAAGAGGA GTGCCCCCTG GAAGCCGGGC TACACAGCAG CAGTAAGGTG 
ACCATATATG AATGTGATGA AGGCTCGGAA CCCCCCGGAT TCTGGGATGC GCTGGGCAGG 
CGAGACCGTA AAGCGTACGA CTGCATGTTG CAAGATCCTG GGAAATTTAA CTTCACGCCA 
CGACTGTTCA GCCTCAGTAG TTCATCGGGA GAGTTTACAG CATCAGAAAT CCTGTATCCT 
TCAAGGCATC CGAATGTGGT TAACTCCATG CCATTTTTAC AAGAAGACCT GTATACGGCC 
TCCCAGCCAG CTTTATTCCT CGTTGACAAC CATCACGAGG TTTACCTGTG GCAAGGTTGG 
TGGCCGTCTG AGAATATTAT TACTGGGTCT GCTCGTATTC GGTGGGATTC AGACAGAAAA 
AGCGCAATGG AGACCGTACT CAGCTACTGC AAAGGAAAGA ATATCAAGAA ACCTCCCAAG 
TCGTATCTAA TCCATGCCGG GCTGGAGCCT CTGACCTTTA CCAACATGTT TCCAAGCTGG 
GAACATAGGG AAGACATTGC CCAAATCACA GAAAAGGATG CGGAAGTCTC TAACCAGATA 
ATCCTTGTAG AAGATGTACT CGCTAAACTT TGTAAAAGAA TCTACCCCTT GGCAGATCTT 
CTTGCCCGCC CGTTGCCCGA AGGAGTCGAC CCTCTCAAGC TTGAGCTCTA TCTGTCAGAT 
AAAGACTTTG AGGTTGCGTT AGAAATGACA AGAGAAGAAT ACAACACACT GCCGCCCTGG 
AAACAGGTTA ATACAAAGAA AGCCAAAGGA CTTTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

svil ( XM_012965657.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821111.1 Xenopus tropicalis supervillin (svil), transcript variant X2, mRNA.

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CloneID	OXa29955
Clone ID Related Accession (Same CDS sequence)	XM_012965657.2 , XM_012965657.3
Accession Version	XM_012965657.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	supervillin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965657.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCGAA ACCGCTGGAT CTCCAGCTAT GCTGGGAGTA TTATCTGTCA TGAGGATCCA 
GGATTTGATC GAGAAGGTAC ATTTCAACAC CGAACGTCAC GTCTTCCACA GAAAAAGCCG 
CCAAGTTTTC AGAAGAGTTT TGTTATTGAA GTTACTGGGG ATTTCACAAA CAACTCCAGG 
GAAACTGATG CACATCTGCT AGCTGTACTT CCAAAAGTTT CAGAACTAAG GAAACTCTTT 
GAGCCAAGCA GGAGATCCAT TTCAGAGATG AAAAGAAAAG AAAGAATAGC CAGACGTTTG 
GAAGGAATTG ATAACGACCC ACAACCAATT ATGCTACAGA CACGTTTATT GGAAGAAGAT 
ATGCCACGAT ATATGCGCGC AACAGATGCA TATAATCCAC ACATAGGTCA TGCAAATGAA 
GACACTGCCT CAGAGTCATC AATAGAAACA CATTCTAGAT GGCAAAACCG CTGGCAGCCA 
AGCACTGAAT CATCTTCTAT GTCAGGTCAA ATGGCGGTAG AGGAAACAGA ATCAAAAGCA 
GAAAGGATAG CTCGATACAA GGCGGAGCGA AGACGGCAAT TGGCAGAAAG ATATGGTGTC 
CCTATGGATT CCGAAGCGGA TTCCGAATAC CAGTCAAGGT ACACGAGAAT AAGGAAAGAC 
GGTGAGATCT CTGAAAAGAA ATCTGTGTTA GAGGAAGATG AAATTAAAGA GAATAGCTCA 
GATTATTTAT ACCGTCAAGA CATTAAGGAG CCTCGCAGAT TTCGAATGTA CGAATCAAAG 
CATCGGGACA TTAATGAGAA TGTGTCGGAG AGAGATGCTC TCAAAAATGT GGAAAACAAT 
AATAATCAAT ATCGAGAAAT ATCCGAGTTG GATGGATCTA CCTTTTCGTG CATGGGACAA 
GCCATCACAG TTGGGGAAGC ACCCAGGTCT CCAAAGTTCC CTCGTCGTGC ATCAGAGAAA 
TCACTTCAGC TTTCCTCCTC TCCACCATCT GAACAGCTAC TGCTTAGCGA CTCCAGACAA 
GGAACAGGAA AACAGGCACC AGAGTGGTCT CTCCAAAAAG ATTCCGATGG GGACACAGCG 
TCTCTCATCA GCTGGCCGTC CAGAGTTAAA GTTAGAGAGA GGCTGGGGAG AGAACATATT 
CACGGATTTC CCAAAACTGA CACAGAGATC TCAACGCAAA GGAAACATCC ACCCGGGCCT 
GCCCACCCTG TTCCTTTCCA GTCGGAAACC TCTGCTTTTC ATAGGGTTTA TAAACAGCCG 
GCATTAGTTC ACCAGCCCAT TCATGGCTAC ATCCAGCCTG CTAGTGTTGC TCATACACCT 
AAAATTGTGG CACCTCATGC ACCTGATTCG AGTGTCATGG TGTCCGCTTT TACTACCTCC 
TTAAAAAATC CCAGATCTTC CAGTGATGTG TATATTGAGA AATCCCACCA TGCATTTCAA 
GGTCAGGACA ATATCGTAGA GCCATGCAAG CAGGTGATCA GCAATGGCAA ATCGCACAGT 
ATGTATGAAG AGAAGCCAAC ATATATTTCA TCTGCCAGCA AGCCAAGAAA CGAGACCTTG 
CCTGGGCATA AAGACCCAGA CCTGCATGTT TCACACAGTT TACCACTTCA GAGACAAACC 
TCTGGAAACT TTGGGAAAAG CGAAAAGGTC TTGTTCCTTC AGAGTGGCAG ATCCCAGGAC 
CATACCAAGG AAGTATCTAC GATGAAGCAT AAAATGCTGA CCCGTTCAAT GTCTGACACC 
ACCAACTCCC AAAAGATGGA AGCTTTTAAG ATAAAGGACT CCGATGTCCA GAAAGAGATG 
GTGTTTTTGC CTTCTGAAGA CCATGTTGCC ATTCCAAATG GAGAAGCCGG CATTGTCAAT 
ACCAAAGTGT CAGTTGCTCA GCTTAGGAGT GCATATTTGG AGACGGCCAG CCACGCACAA 
AAATCTGAGT TTGAACCTAA GGCTGAAATG CCAACGTCAG GTGCTTTGTC TGCTACTACT 
GAGCGGGAAA GAGGCCCTCG AAGGCCGAGA CTTTATTTCT CTCCTGGTGA AAGTAGAAAG 
ACCTCTGAAA GATTTAGAAC CCAGCCTGTA ACGTCAGCTG AGCGAAATCA GATGGATAGA 
TCAAGCTTCC AGTCACCAGA GCAGATTGCT GAAGAAGAAG CAAAAGTGGA TGAAAGAGCC 
AAACTTAGCG TGGCTGCGAA AAGGCTTCTA TTCAGGGAAA TGGAAAAATC TCTGGAGTCG 
AAACCAGCCA AGCCACGTGC CAGAAATGCA GCAGTGGAGA GGAGGCTTCG CCGAGCACAA 
GACAGGTCTC GGACGCAACC CGTTACCAGT GAAGAGGTGG TCATTGCTGC TACTGAGCCT 
AGTGCTGCTT CGCACACTGT GACCACACAC ACGGCCCTCC CTCGAATACC TAGCCCCACT 
GTAACTAAGA GCTCTGTACA CCATATCAGT TTGCAGGCAT CATCCCATAA AAGCACAATA 
TCAAAGCAAG AGGAGATACA AAAGGAAATA CACGATGACC AGAGCATTCC CGAAGAGCCT 
GATAATTCCT CGCTTTCCTT GGCTGAAAAA ATGGCATTAT TTAATAAGCT TTCCCAGCCA 
ATAACAAAAG AGGTGTCTAC GAGGAGCAGA ATGGATATTC GACAAAGGCG AATGAATGCG 
CGTTACCAGA CTCAGCCCGT CACCCTTGTG GAGGTTGAAC AGTATAAGAA TGGAGGCGAA 
AAGATCGGCC CCTTATCTCA CAGTGTTGTA ACTTCCGTAG CCACCCTAAC CTCTCGGCCG 
TCTCCAGTTA GCCCTGGGGA TATGCATACT GCAAAGTCCT CCAGTTATGA AGAGAGAATA 
TCAAGTGTCT CCAGCACTTC AGCCCTGCTG CACACTTCAC TGGAAACAGG AAGCACTTCG 
GAAAGGAGCT TGCTGCTCTC GGAGCCCTGG AGTCCCCCAA GTGTGAAAGA TAAAGTATAT 
GAAAAGGAAC CGAGGAAACC AATGTTGGAA GAGACAAGGG TCTCTGCAGC AGAAGATAGC 
AGCAGCTTCG GAAAATGCAG CTCGCTCCAT AAGGAAAAAA CCCACCCCGC GCTTGATGTG 
GCAAAGGAGG AAGAACATGT TGAGGAGCAG AAAGTGCCTG GGCATGTTGC CTCAAGTAGC 
AGCAGAGTGG AGTTGCTCAG CCCCCAGAAG CTGTCCCAGC CTGCTGAGCA TTCAGAATCC 
CAAGCAAAGC CTGAAAGTGC CCTGCAGGTA GACGCTGAAG ATAAGAAGAG CCTACATCCC 
GAGCCCAGCA TGAGAAGAAG GAAAGAGATC CACGAGATTG TTGAGCCGGC CTCTGAACTC 
CTTGGCACCA GCGCTGCAAA GACCGTTTCT CACACTCCAG CTGCTGCTCC TGCTGCGGTG 
TCCTACGGAC CACAGGCATC AGAGCTAAGT GAGGAAAAGC CCGAGGAGGA AACAGCTGAG 
ATCTCCTCCA CCATCAGCAA CAGGTCCAAC GAGGATCTCG ATACGCCCGG CAAAGCCATG 
TCCATCAAAG ACAGGCTGGC GCTGCTAAAG AAGAGTGGGG AAGAGGATTG GAAAAACAGG 
CTTAACAAAA AGCAGGAGTA CAGCAAAGTG TCTGTCATCG AGCGCAGCAC GCTCACCCAG 
CTCCATGAGA TTGAGCAGTC GCTGACAAAG AAGGAACCTG TCTATGCTTC TGTTTATTCT 
CCTGCTCTAC CTGCCGCCCA TAAATGCCAG TATTTTGTTC CTATACATCA GGTCAGTAAT 
GCTACGACCC TGGGGCAGTC CAGCCCTGTG ATGTTCGATG AGCGGCACCC CTGGAGATGG 
AAGCAAAGGG TGACTGACGG CAATGGGCTT CACGTGTCTA TTGAAGAAAG GAAGCAGCAG 
ATTACAGTCA GGGAGGAGGC CTGGAAAACC AAAGGCATAG GGGCAGCAAA TGATTCCACA 
CAATTTACAG TGGCCGGCCG CATGGTTAAG AAAGGTCTGG CCTCACCTAC AGCCATTACC 
CCCGTCGCAT CTCCAATGTC TCTCAAATTT AAAACTCCAG TTTCCAAGCC TCTGGAAGAA 
ATTGAAGCCA AACCTGATAT GAATTTGGAA TCTGACAGGA AGTTAGAAAA ACTAGAATCA 
TTTTTGGGAA AACTGCACAA CAGGGTTGGA GGGCTACAAG ATACCACACT GTCAGTCACA 
GGCCAATCAG TGAAGGAGGT GATGAAGGTG GACGATGATG AAACTTTCGG AAGGTTCTAC 
CGCCCTTTTG AATTCTCCAC TGCCCACGTG CCATTTGATT TTGAAGATGA TTTTGATCTT 
CTCTTCAGTT CGGAGGAACC AAAGCTGACA TCTGCTGTAG CAGAACACAA GCGTGCCGTC 
CGGCCAGCCC GCCGGGTGCA AGCGTCGGGA AACCCACTGA AGATGCTTGC GGCCAGGGAG 
GACATCCTAC AGGAGTACAC AGAGACGCGG CTGAACGTGG CGTTCATGGA GTCCAAGAGA 
ATGAAAGTAG AAAAAATGTC CAAGAACTCA AACTTCTCAG AAGTCGCACT TGCAGGCCTG 
GCAAGCAAAG AAAACTTTAC CAGCGTAAGC CTGCGGAGTG TCAATATGAC CGAGCAGCAC 
TCCAACAACA GCGCCGTGCC ATACAAGAAG CTTATGCTAG TTCAGATTAA AGGACGCAGG 
CACGTTCAGT CGAGGCTGGT GGAACCGCGT GCATCCTCTC TGAATAGCGG AGACTGCTTC 
CTGCTGATCA CCCCGCACCA CTGCTTCTTG TGGGTCGGAG AGTTTGCAAA CGTCATTGAG 
AAAGCCAAGG CTTCTGAGCT GGCATCAATA ATTCAGACCA AGAGGGAACT TGGATGCAGG 
GCATCGTATG TGCAAACAGT CGAGGAAGGA ATAAATACCC ACACGCATGG AGCCAAGGAT 
TTTTGGAAAC TTCTGAGCGG TCAGACTGAA TCTCAAGCTG CTGGGAGCCC AGAGGAGGAT 
GAGAGATATG AAAATGCCAT CATAGAAACA AACTGTATTT ATCGATTGCT GGAAGATAAG 
CTGGTCCCTG TTGATGAATT TTGGGGCAAA GTACCTAAGT GTTCACTTTT AGAGTCTAAA 
GAGGTGCTTG TGTTTGATTT TGGAAGTGAA GTGTATGTGT GGCATGGGAA GGAGGTGACC 
TTGGCACAGA GGAAGGTGGC GTTCCAGTTA GCCAAACATC TCTGGAATGG AGCTTTTGAC 
TATGAAAATT GTGATATCAA TCCTCTGGAC CCTGGGGAAT GCAATTCTCT CATTCCAAGG 
AAAGGGCACG GGCGCCCTGA TTGGGCAATA TTTGGAAGGC TGACAGAGCA TAATGAAACC 
ATACTGTTCA AGGAAAAGTT CCTGGATTGG ACAGAAATGA AGAAACCCAA TGAGAAGGGT 
GTGTGCGAGG ACTCACCGAA GCTTAAGGAA GACCCACAAT CTGATGTGAA GCCTTATGAC 
ATCATGCTGA TGGTCCCAGT TCCCCAGAAA TCTGTGGGTA CGGTTCTTGA TGGATTAAAT 
GTCGCCCGTG GTTATGGACT TATTGAGGGA GATGATTGCC GCCAGTATGA AATCTCAACC 
GTCTCGGTGG ATGTCTGGCA TATCCTTGAA TTCGACTACA GCCGGCTGCC GAAGCAGAGC 
ATTGGCCAGT TCCACGAGGG AGACGCCTAT GTGGTAAAAT GGAAGTACTT TGTGAGCACA 
ACAGTTGGAC AGCGCCAGAA AACCGAGCAC GTCCGGATAG CGGGTAAAGA GAAGTGCGCC 
TACTTCTTCT GGCAGGGACG GAACTCGCCC GTAAGTGAGA AGGGAACCTC AGCGCTGATG 
ACTGTGGAGC TTGATGAAGA GCGGGGAGCA CAGGTCCAGG TGCTTCAGGG AAAAGAGCCC 
CCCTGTTTCT TACAATGTTT CCAGGGAGGA ATGATTGTGC ATGCTGGGAG ACGGGAAGAA 
GAGGAAGAGA ACACGCAAAG TGACTGGCGG TTGTACTGTG TACGTGGGGA GGTGCCGGTG 
GAAGGGAATT TGATGGAAGT CGCTTGTCAC TGCAGCAGCC TGCGCTCCAG GACCTCCTTG 
ATACTCCTCA GTATAAATAA AGCGCTCATC TACCTTTGGC ACGGCTGCAA AGTCCAAATC 
CATGCCAAGG AAGTGGGACG GACGGCAGCC AATAAAATAA AAGAGGAGTG CCCCCTGGAA 
GCCGGGCTAC ACAGCAGCAG TAAGGTGACC ATATATGAAT GTGATGAAGG CTCGGAACCC 
CCCGGATTCT GGGATGCGCT GGGCAGGCGA GACCGTAAAG CGTACGACTG CATGTTGCAA 
GATCCTGGGA AATTTAACTT CACGCCACGA CTGTTCAGCC TCAGTAGTTC ATCGGGAGAG 
TTTACAGCAT CAGAAATCCT GTATCCTTCA AGGCATCCGA ATGTGGTTAA CTCCATGCCA 
TTTTTACAAG AAGACCTGTA TACGGCCTCC CAGCCAGCTT TATTCCTCGT TGACAACCAT 
CACGAGGTTT ACCTGTGGCA AGGTTGGTGG CCGTCTGAGA ATATTATTAC TGGGTCTGCT 
CGTATTCGGT GGGATTCAGA CAGAAAAAGC GCAATGGAGA CCGTACTCAG CTACTGCAAA 
GGAAAGAATA TCAAGAAACC TCCCAAGTCG TATCTAATCC ATGCCGGGCT GGAGCCTCTG 
ACCTTTACCA ACATGTTTCC AAGCTGGGAA CATAGGGAAG ACATTGCCCA AATCACAGAA 
AAGGATGCGG AAGTCTCTAA CCAGATAATC CTTGTAGAAG ATGTACTCGC TAAACTTTGT 
AAAAGAATCT ACCCCTTGGC AGATCTTCTT GCCCGCCCGT TGCCCGAAGG AGTCGACCCT 
CTCAAGCTTG AGCTCTATCT GTCAGATAAA GACTTTGAGG TTGCGTTAGA AATGACAAGA 
GAAGAATACA ACACACTGCC GCCCTGGAAA CAGGTTAATA CAAAGAAAGC CAAAGGACTT 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821111.1
CDS	90..6995
Translation	MFRNRWISSYAGSIICHEDPGFDREGTFQHRTSRLPQKKPPSFQKSFVIEVTGDFTNNSRETDAHLLAVLPKVSELRKLFEPSRRSISEMKRKERIARRLEGIDNDPQPIMLQTRLLEEDMPRYMRATDAYNPHIGHANEDTASESSIETHSRWQNRWQPSTESSSMSGQMAVEETESKAERIARYKAERRRQLAERYGVPMDSEADSEYQSRYTRIRKDGEISEKKSVLEEDEIKENSSDYLYRQDIKEPRRFRMYESKHRDINENVSERDALKNVENNNNQYREISELDGSTFSCMGQAITVGEAPRSPKFPRRASEKSLQLSSSPPSEQLLLSDSRQGTGKQAPEWSLQKDSDGDTASLISWPSRVKVRERLGREHIHGFPKTDTEISTQRKHPPGPAHPVPFQSETSAFHRVYKQPALVHQPIHGYIQPASVAHTPKIVAPHAPDSSVMVSAFTTSLKNPRSSSDVYIEKSHHAFQGQDNIVEPCKQVISNGKSHSMYEEKPTYISSASKPRNETLPGHKDPDLHVSHSLPLQRQTSGNFGKSEKVLFLQSGRSQDHTKEVSTMKHKMLTRSMSDTTNSQKMEAFKIKDSDVQKEMVFLPSEDHVAIPNGEAGIVNTKVSVAQLRSAYLETASHAQKSEFEPKAEMPTSGALSATTERERGPRRPRLYFSPGESRKTSERFRTQPVTSAERNQMDRSSFQSPEQIAEEEAKVDERAKLSVAAKRLLFREMEKSLESKPAKPRARNAAVERRLRRAQDRSRTQPVTSEEVVIAATEPSAASHTVTTHTALPRIPSPTVTKSSVHHISLQASSHKSTISKQEEIQKEIHDDQSIPEEPDNSSLSLAEKMALFNKLSQPITKEVSTRSRMDIRQRRMNARYQTQPVTLVEVEQYKNGGEKIGPLSHSVVTSVATLTSRPSPVSPGDMHTAKSSSYEERISSVSSTSALLHTSLETGSTSERSLLLSEPWSPPSVKDKVYEKEPRKPMLEETRVSAAEDSSSFGKCSSLHKEKTHPALDVAKEEEHVEEQKVPGHVASSSSRVELLSPQKLSQPAEHSESQAKPESALQVDAEDKKSLHPEPSMRRRKEIHEIVEPASELLGTSAAKTVSHTPAAAPAAVSYGPQASELSEEKPEEETAEISSTISNRSNEDLDTPGKAMSIKDRLALLKKSGEEDWKNRLNKKQEYSKVSVIERSTLTQLHEIEQSLTKKEPVYASVYSPALPAAHKCQYFVPIHQVSNATTLGQSSPVMFDERHPWRWKQRVTDGNGLHVSIEERKQQITVREEAWKTKGIGAANDSTQFTVAGRMVKKGLASPTAITPVASPMSLKFKTPVSKPLEEIEAKPDMNLESDRKLEKLESFLGKLHNRVGGLQDTTLSVTGQSVKEVMKVDDDETFGRFYRPFEFSTAHVPFDFEDDFDLLFSSEEPKLTSAVAEHKRAVRPARRVQASGNPLKMLAAREDILQEYTETRLNVAFMESKRMKVEKMSKNSNFSEVALAGLASKENFTSVSLRSVNMTEQHSNNSAVPYKKLMLVQIKGRRHVQSRLVEPRASSLNSGDCFLLITPHHCFLWVGEFANVIEKAKASELASIIQTKRELGCRASYVQTVEEGINTHTHGAKDFWKLLSGQTESQAAGSPEEDERYENAIIETNCIYRLLEDKLVPVDEFWGKVPKCSLLESKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGAFDYENCDINPLDPGECNSLIPRKGHGRPDWAIFGRLTEHNETILFKEKFLDWTEMKKPNEKGVCEDSPKLKEDPQSDVKPYDIMLMVPVPQKSVGTVLDGLNVARGYGLIEGDDCRQYEISTVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKYFVSTTVGQRQKTEHVRIAGKEKCAYFFWQGRNSPVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMIVHAGRREEEEENTQSDWRLYCVRGEVPVEGNLMEVACHCSSLRSRTSLILLSINKALIYLWHGCKVQIHAKEVGRTAANKIKEECPLEAGLHSSSKVTIYECDEGSEPPGFWDALGRRDRKAYDCMLQDPGKFNFTPRLFSLSSSSGEFTASEILYPSRHPNVVNSMPFLQEDLYTASQPALFLVDNHHEVYLWQGWWPSENIITGSARIRWDSDRKSAMETVLSYCKGKNIKKPPKSYLIHAGLEPLTFTNMFPSWEHREDIAQITEKDAEVSNQIILVEDVLAKLCKRIYPLADLLARPLPEGVDPLKLELYLSDKDFEVALEMTREEYNTLPPWKQVNTKKAKGLF

Target ORF information:

RefSeq Version	XM_012965657.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis supervillin (svil), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965657.2

ORF Insert Sequence:

ATGTTCCGAA ACCGCTGGAT CTCCAGCTAT GCTGGGAGTA TTATCTGTCA TGAGGATCCA 
GGATTTGATC GAGAAGGTAC ATTTCAACAC CGAACGTCAC GTCTTCCACA GAAAAAGCCG 
CCAAGTTTTC AGAAGAGTTT TGTTATTGAA GTTACTGGGG ATTTCACAAA CAACTCCAGG 
GAAACTGATG CACATCTGCT AGCTGTACTT CCAAAAGTTT CAGAACTAAG GAAACTCTTT 
GAGCCAAGCA GGAGATCCAT TTCAGAGATG AAAAGAAAAG AAAGAATAGC CAGACGTTTG 
GAAGGAATTG ATAACGACCC ACAACCAATT ATGCTACAGA CACGTTTATT GGAAGAAGAT 
ATGCCACGAT ATATGCGCGC AACAGATGCA TATAATCCAC ACATAGGTCA TGCAAATGAA 
GACACTGCCT CAGAGTCATC AATAGAAACA CATTCTAGAT GGCAAAACCG CTGGCAGCCA 
AGCACTGAAT CATCTTCTAT GTCAGGTCAA ATGGCGGTAG AGGAAACAGA ATCAAAAGCA 
GAAAGGATAG CTCGATACAA GGCGGAGCGA AGACGGCAAT TGGCAGAAAG ATATGGTGTC 
CCTATGGATT CCGAAGCGGA TTCCGAATAC CAGTCAAGGT ACACGAGAAT AAGGAAAGAC 
GGTGAGATCT CTGAAAAGAA ATCTGTGTTA GAGGAAGATG AAATTAAAGA GAATAGCTCA 
GATTATTTAT ACCGTCAAGA CATTAAGGAG CCTCGCAGAT TTCGAATGTA CGAATCAAAG 
CATCGGGACA TTAATGAGAA TGTGTCGGAG AGAGATGCTC TCAAAAATGT GGAAAACAAT 
AATAATCAAT ATCGAGAAAT ATCCGAGTTG GATGGATCTA CCTTTTCGTG CATGGGACAA 
GCCATCACAG TTGGGGAAGC ACCCAGGTCT CCAAAGTTCC CTCGTCGTGC ATCAGAGAAA 
TCACTTCAGC TTTCCTCCTC TCCACCATCT GAACAGCTAC TGCTTAGCGA CTCCAGACAA 
GGAACAGGAA AACAGGCACC AGAGTGGTCT CTCCAAAAAG ATTCCGATGG GGACACAGCG 
TCTCTCATCA GCTGGCCGTC CAGAGTTAAA GTTAGAGAGA GGCTGGGGAG AGAACATATT 
CACGGATTTC CCAAAACTGA CACAGAGATC TCAACGCAAA GGAAACATCC ACCCGGGCCT 
GCCCACCCTG TTCCTTTCCA GTCGGAAACC TCTGCTTTTC ATAGGGTTTA TAAACAGCCG 
GCATTAGTTC ACCAGCCCAT TCATGGCTAC ATCCAGCCTG CTAGTGTTGC TCATACACCT 
AAAATTGTGG CACCTCATGC ACCTGATTCG AGTGTCATGG TGTCCGCTTT TACTACCTCC 
TTAAAAAATC CCAGATCTTC CAGTGATGTG TATATTGAGA AATCCCACCA TGCATTTCAA 
GGTCAGGACA ATATCGTAGA GCCATGCAAG CAGGTGATCA GCAATGGCAA ATCGCACAGT 
ATGTATGAAG AGAAGCCAAC ATATATTTCA TCTGCCAGCA AGCCAAGAAA CGAGACCTTG 
CCTGGGCATA AAGACCCAGA CCTGCATGTT TCACACAGTT TACCACTTCA GAGACAAACC 
TCTGGAAACT TTGGGAAAAG CGAAAAGGTC TTGTTCCTTC AGAGTGGCAG ATCCCAGGAC 
CATACCAAGG AAGTATCTAC GATGAAGCAT AAAATGCTGA CCCGTTCAAT GTCTGACACC 
ACCAACTCCC AAAAGATGGA AGCTTTTAAG ATAAAGGACT CCGATGTCCA GAAAGAGATG 
GTGTTTTTGC CTTCTGAAGA CCATGTTGCC ATTCCAAATG GAGAAGCCGG CATTGTCAAT 
ACCAAAGTGT CAGTTGCTCA GCTTAGGAGT GCATATTTGG AGACGGCCAG CCACGCACAA 
AAATCTGAGT TTGAACCTAA GGCTGAAATG CCAACGTCAG GTGCTTTGTC TGCTACTACT 
GAGCGGGAAA GAGGCCCTCG AAGGCCGAGA CTTTATTTCT CTCCTGGTGA AAGTAGAAAG 
ACCTCTGAAA GATTTAGAAC CCAGCCTGTA ACGTCAGCTG AGCGAAATCA GATGGATAGA 
TCAAGCTTCC AGTCACCAGA GCAGATTGCT GAAGAAGAAG CAAAAGTGGA TGAAAGAGCC 
AAACTTAGCG TGGCTGCGAA AAGGCTTCTA TTCAGGGAAA TGGAAAAATC TCTGGAGTCG 
AAACCAGCCA AGCCACGTGC CAGAAATGCA GCAGTGGAGA GGAGGCTTCG CCGAGCACAA 
GACAGGTCTC GGACGCAACC CGTTACCAGT GAAGAGGTGG TCATTGCTGC TACTGAGCCT 
AGTGCTGCTT CGCACACTGT GACCACACAC ACGGCCCTCC CTCGAATACC TAGCCCCACT 
GTAACTAAGA GCTCTGTACA CCATATCAGT TTGCAGGCAT CATCCCATAA AAGCACAATA 
TCAAAGCAAG AGGAGATACA AAAGGAAATA CACGATGACC AGAGCATTCC CGAAGAGCCT 
GATAATTCCT CGCTTTCCTT GGCTGAAAAA ATGGCATTAT TTAATAAGCT TTCCCAGCCA 
ATAACAAAAG AGGTGTCTAC GAGGAGCAGA ATGGATATTC GACAAAGGCG AATGAATGCG 
CGTTACCAGA CTCAGCCCGT CACCCTTGTG GAGGTTGAAC AGTATAAGAA TGGAGGCGAA 
AAGATCGGCC CCTTATCTCA CAGTGTTGTA ACTTCCGTAG CCACCCTAAC CTCTCGGCCG 
TCTCCAGTTA GCCCTGGGGA TATGCATACT GCAAAGTCCT CCAGTTATGA AGAGAGAATA 
TCAAGTGTCT CCAGCACTTC AGCCCTGCTG CACACTTCAC TGGAAACAGG AAGCACTTCG 
GAAAGGAGCT TGCTGCTCTC GGAGCCCTGG AGTCCCCCAA GTGTGAAAGA TAAAGTATAT 
GAAAAGGAAC CGAGGAAACC AATGTTGGAA GAGACAAGGG TCTCTGCAGC AGAAGATAGC 
AGCAGCTTCG GAAAATGCAG CTCGCTCCAT AAGGAAAAAA CCCACCCCGC GCTTGATGTG 
GCAAAGGAGG AAGAACATGT TGAGGAGCAG AAAGTGCCTG GGCATGTTGC CTCAAGTAGC 
AGCAGAGTGG AGTTGCTCAG CCCCCAGAAG CTGTCCCAGC CTGCTGAGCA TTCAGAATCC 
CAAGCAAAGC CTGAAAGTGC CCTGCAGGTA GACGCTGAAG ATAAGAAGAG CCTACATCCC 
GAGCCCAGCA TGAGAAGAAG GAAAGAGATC CACGAGATTG TTGAGCCGGC CTCTGAACTC 
CTTGGCACCA GCGCTGCAAA GACCGTTTCT CACACTCCAG CTGCTGCTCC TGCTGCGGTG 
TCCTACGGAC CACAGGCATC AGAGCTAAGT GAGGAAAAGC CCGAGGAGGA AACAGCTGAG 
ATCTCCTCCA CCATCAGCAA CAGGTCCAAC GAGGATCTCG ATACGCCCGG CAAAGCCATG 
TCCATCAAAG ACAGGCTGGC GCTGCTAAAG AAGAGTGGGG AAGAGGATTG GAAAAACAGG 
CTTAACAAAA AGCAGGAGTA CAGCAAAGTG TCTGTCATCG AGCGCAGCAC GCTCACCCAG 
CTCCATGAGA TTGAGCAGTC GCTGACAAAG AAGGAACCTG TCTATGCTTC TGTTTATTCT 
CCTGCTCTAC CTGCCGCCCA TAAATGCCAG TATTTTGTTC CTATACATCA GGTCAGTAAT 
GCTACGACCC TGGGGCAGTC CAGCCCTGTG ATGTTCGATG AGCGGCACCC CTGGAGATGG 
AAGCAAAGGG TGACTGACGG CAATGGGCTT CACGTGTCTA TTGAAGAAAG GAAGCAGCAG 
ATTACAGTCA GGGAGGAGGC CTGGAAAACC AAAGGCATAG GGGCAGCAAA TGATTCCACA 
CAATTTACAG TGGCCGGCCG CATGGTTAAG AAAGGTCTGG CCTCACCTAC AGCCATTACC 
CCCGTCGCAT CTCCAATGTC TCTCAAATTT AAAACTCCAG TTTCCAAGCC TCTGGAAGAA 
ATTGAAGCCA AACCTGATAT GAATTTGGAA TCTGACAGGA AGTTAGAAAA ACTAGAATCA 
TTTTTGGGAA AACTGCACAA CAGGGTTGGA GGGCTACAAG ATACCACACT GTCAGTCACA 
GGCCAATCAG TGAAGGAGGT GATGAAGGTG GACGATGATG AAACTTTCGG AAGGTTCTAC 
CGCCCTTTTG AATTCTCCAC TGCCCACGTG CCATTTGATT TTGAAGATGA TTTTGATCTT 
CTCTTCAGTT CGGAGGAACC AAAGCTGACA TCTGCTGTAG CAGAACACAA GCGTGCCGTC 
CGGCCAGCCC GCCGGGTGCA AGCGTCGGGA AACCCACTGA AGATGCTTGC GGCCAGGGAG 
GACATCCTAC AGGAGTACAC AGAGACGCGG CTGAACGTGG CGTTCATGGA GTCCAAGAGA 
ATGAAAGTAG AAAAAATGTC CAAGAACTCA AACTTCTCAG AAGTCGCACT TGCAGGCCTG 
GCAAGCAAAG AAAACTTTAC CAGCGTAAGC CTGCGGAGTG TCAATATGAC CGAGCAGCAC 
TCCAACAACA GCGCCGTGCC ATACAAGAAG CTTATGCTAG TTCAGATTAA AGGACGCAGG 
CACGTTCAGT CGAGGCTGGT GGAACCGCGT GCATCCTCTC TGAATAGCGG AGACTGCTTC 
CTGCTGATCA CCCCGCACCA CTGCTTCTTG TGGGTCGGAG AGTTTGCAAA CGTCATTGAG 
AAAGCCAAGG CTTCTGAGCT GGCATCAATA ATTCAGACCA AGAGGGAACT TGGATGCAGG 
GCATCGTATG TGCAAACAGT CGAGGAAGGA ATAAATACCC ACACGCATGG AGCCAAGGAT 
TTTTGGAAAC TTCTGAGCGG TCAGACTGAA TCTCAAGCTG CTGGGAGCCC AGAGGAGGAT 
GAGAGATATG AAAATGCCAT CATAGAAACA AACTGTATTT ATCGATTGCT GGAAGATAAG 
CTGGTCCCTG TTGATGAATT TTGGGGCAAA GTACCTAAGT GTTCACTTTT AGAGTCTAAA 
GAGGTGCTTG TGTTTGATTT TGGAAGTGAA GTGTATGTGT GGCATGGGAA GGAGGTGACC 
TTGGCACAGA GGAAGGTGGC GTTCCAGTTA GCCAAACATC TCTGGAATGG AGCTTTTGAC 
TATGAAAATT GTGATATCAA TCCTCTGGAC CCTGGGGAAT GCAATTCTCT CATTCCAAGG 
AAAGGGCACG GGCGCCCTGA TTGGGCAATA TTTGGAAGGC TGACAGAGCA TAATGAAACC 
ATACTGTTCA AGGAAAAGTT CCTGGATTGG ACAGAAATGA AGAAACCCAA TGAGAAGGGT 
GTGTGCGAGG ACTCACCGAA GCTTAAGGAA GACCCACAAT CTGATGTGAA GCCTTATGAC 
ATCATGCTGA TGGTCCCAGT TCCCCAGAAA TCTGTGGGTA CGGTTCTTGA TGGATTAAAT 
GTCGCCCGTG GTTATGGACT TATTGAGGGA GATGATTGCC GCCAGTATGA AATCTCAACC 
GTCTCGGTGG ATGTCTGGCA TATCCTTGAA TTCGACTACA GCCGGCTGCC GAAGCAGAGC 
ATTGGCCAGT TCCACGAGGG AGACGCCTAT GTGGTAAAAT GGAAGTACTT TGTGAGCACA 
ACAGTTGGAC AGCGCCAGAA AACCGAGCAC GTCCGGATAG CGGGTAAAGA GAAGTGCGCC 
TACTTCTTCT GGCAGGGACG GAACTCGCCC GTAAGTGAGA AGGGAACCTC AGCGCTGATG 
ACTGTGGAGC TTGATGAAGA GCGGGGAGCA CAGGTCCAGG TGCTTCAGGG AAAAGAGCCC 
CCCTGTTTCT TACAATGTTT CCAGGGAGGA ATGATTGTGC ATGCTGGGAG ACGGGAAGAA 
GAGGAAGAGA ACACGCAAAG TGACTGGCGG TTGTACTGTG TACGTGGGGA GGTGCCGGTG 
GAAGGGAATT TGATGGAAGT CGCTTGTCAC TGCAGCAGCC TGCGCTCCAG GACCTCCTTG 
ATACTCCTCA GTATAAATAA AGCGCTCATC TACCTTTGGC ACGGCTGCAA AGTCCAAATC 
CATGCCAAGG AAGTGGGACG GACGGCAGCC AATAAAATAA AAGAGGAGTG CCCCCTGGAA 
GCCGGGCTAC ACAGCAGCAG TAAGGTGACC ATATATGAAT GTGATGAAGG CTCGGAACCC 
CCCGGATTCT GGGATGCGCT GGGCAGGCGA GACCGTAAAG CGTACGACTG CATGTTGCAA 
GATCCTGGGA AATTTAACTT CACGCCACGA CTGTTCAGCC TCAGTAGTTC ATCGGGAGAG 
TTTACAGCAT CAGAAATCCT GTATCCTTCA AGGCATCCGA ATGTGGTTAA CTCCATGCCA 
TTTTTACAAG AAGACCTGTA TACGGCCTCC CAGCCAGCTT TATTCCTCGT TGACAACCAT 
CACGAGGTTT ACCTGTGGCA AGGTTGGTGG CCGTCTGAGA ATATTATTAC TGGGTCTGCT 
CGTATTCGGT GGGATTCAGA CAGAAAAAGC GCAATGGAGA CCGTACTCAG CTACTGCAAA 
GGAAAGAATA TCAAGAAACC TCCCAAGTCG TATCTAATCC ATGCCGGGCT GGAGCCTCTG 
ACCTTTACCA ACATGTTTCC AAGCTGGGAA CATAGGGAAG ACATTGCCCA AATCACAGAA 
AAGGATGCGG AAGTCTCTAA CCAGATAATC CTTGTAGAAG ATGTACTCGC TAAACTTTGT 
AAAAGAATCT ACCCCTTGGC AGATCTTCTT GCCCGCCCGT TGCCCGAAGG AGTCGACCCT 
CTCAAGCTTG AGCTCTATCT GTCAGATAAA GACTTTGAGG TTGCGTTAGA AATGACAAGA 
GAAGAATACA ACACACTGCC GCCCTGGAAA CAGGTTAATA CAAAGAAAGC CAAAGGACTT 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

REQUEST A QUOTE

EMAIL

[email protected]

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

svil cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

REQUEST A QUOTE

EMAIL

PHONE

ONLINE FORM

ONLINE FORM

SUPPORT

COMPANY

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