brca2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	brca2
Entrez Gene ID	100038818
Full Name	BRCA2, DNA repair associated
Synonyms	brcc2,brovca2,facd,fad,fad1,fancb,fancd1
General protein information	Preferred Names BRCA2, DNA repair associated Names LOW QUALITY PROTEIN: breast cancer type 2 susceptibility protein breast cancer 2, early onset
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031897235.1	XP_031753095.1	breast cancer type 2 susceptibility protein isoform X1
XM_018091730.2	XP_017947219.2	breast cancer type 2 susceptibility protein isoform X3
XM_018091730.1	XP_017947219.1	LOW QUALITY PROTEIN: breast cancer type 2 susceptibility protein
XM_031897237.1	XP_031753097.1	breast cancer type 2 susceptibility protein isoform X4
XM_031897234.1	XP_031753094.1	breast cancer type 2 susceptibility protein isoform X1
XM_031897236.1	XP_031753096.1	breast cancer type 2 susceptibility protein isoform X2

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	brca2	XP_002934068.2
Danio rerio (zebrafish)	brca2	NP_001103864.2

Gene Ontology

The following brca2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the brca2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa42865	XM_018091730.1 Latest version!	Xenopus tropicalis breast cancer 2 (brca2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa52805	XM_031897235.1 Latest version!	Xenopus tropicalis BRCA2, DNA repair associated (brca2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa52805	XM_031897234.1 Latest version!	Xenopus tropicalis BRCA2, DNA repair associated (brca2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa52806	XM_031897236.1 Latest version!	Xenopus tropicalis BRCA2, DNA repair associated (brca2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa52807	XM_018091730.2 Latest version!	Xenopus tropicalis BRCA2, DNA repair associated (brca2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa52808	XM_031897237.1 Latest version!	Xenopus tropicalis BRCA2, DNA repair associated (brca2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

brca2 ( XM_018091730.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947219.1 Xenopus tropicalis breast cancer 2 (brca2), mRNA.

Price & Availability↑

CloneID	OXa42865
Clone ID Related Accession (Same CDS sequence)	XM_018091730.1
Accession Version	XM_018091730.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	LOW QUALITY PROTEIN: breast cancer type 2 susceptibility protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGCTGCAC CGCAACTTGG AAAATCTGTC TTCTATGATC TGTTTAGCAC GCATTGCTCT 
CACTCAGATT TAGGGCCTAT CAGTCTAAAC TGGTTTGAAG AGCTCACTGC TGAAGCCTTG 
CCTTACAAGT CAAGAACATG TGAAGATCAT GAATGCAGTT TGGATGATCT CCATGAAAAC 
AACATCAAAA CTCCTAAACT GAAGTCGTCA ATTTATAGCC AATTAGATTC CACTCCAGTC 
ATCTTTAAGG AGCGTTTACT ATCACCTCTT TTTGCTTCTT CTTTGACAGA GCTGGATAAA 
AGGCAGAATG CAACAGATAA TGGAAACAAT GTTAGACTTG AGAAAAGTAA CTGTACCCAA 
ATGCAGCATC AAGCAAGTGA AGTTTTCAGT CCCTCAAGTC GATGCCTCAA CGAAAGTCCT 
GCTGTTATCA AAGAAATATT CAAGACACCA TTACGAAATA AGTACCTACA TAAAACACCA 
CAGTGTGATT GGAAACCTGA CATTTGCAGC AGTCTGTTTT GTACTCCAAA GCTTATGAAG 
AACCAAACTG GATGTATTAA AGAAAGTCTA GGTGCAGAGG TGGATCCAGA GATGTCATGG 
TCTAGTTCAC TAGCTACACC TCCTTCCCCA ACTGTGATAA TTGCACATGC TAATGATCAG 
CCCAGTGGAA ATAAACATGC TGCAATTGTA CAGAGCCTTT TCCCAAATTG TGAGAATATG 
GAAGCGAATA ATTTGCCACC ACCTGAGACG GGCACACAAA ATGAAGACCG TCAAATATGT 
GCAGGACTAG AAAAGCCAGG TTCATGCAGT GCGAGCAAAA CTGTACCAGA AGCGTCTGCC 
ATTTCCTCAA GGGCAGCCTG GAAGAAAACT GTTGCAAATA CAGTGAAGGA CGAGGAGGTT 
TCCAGAACTG TGGAAAATGC TCTGGAAGGA ATGGAAGATG TCCTCTCAAT CTTTTTTGCA 
AGTGAAAAGA CTCCTGGTCT CAGAAAGCTG AAAAATTCTA CCCAAGCTAG GAGAAAAGTG 
GAATCTATTA AGTCTCAGAA GTGCCAAGTT TCATTATTTA AAGCAGAAGA AGGAGAAAGC 
ATTTTGGTCC CTTTAGATGA GCATCATAAA TGTGATCATG AGATGCTTAT GAAACCTGAA 
CAAGACTACA AAATGAATGC TGTTAACCAA AAAATAGCCA AAGAAACTAC TCCATATGAA 
TGGTCACAGC TAAATATATG TGAGTTGGAT ATAACTCAAT CACAGGATAG CACTTTGCAT 
GCAGCAAATC TATGTTGTGA AGATATCACT GTCAACAAAA GTGATACATG TAATATTACT 
GAAGCCGAAA AGCAGGAGGA AGTCAATGAG CCAAGCACCG ACAATGTACT GTCTAATAAA 
GTTGGCAAAG AAAGCACTTT AAATGTGAAT TGTATTAATG CTGATTTGAA TAACATGGCT 
TCATCCTCAA ATAACTCTGT TCACTCACTG AATCTTTCTC AATGTGAAAA AATGGATTCT 
TCTGAAATTT CTAACAGTGA AGTTGGTTGT ATAACAAAAC TAACAACCCA CCCAGCAAAA 
ATAACAACTG GTGAATTAAG TTCAGTAGAT GACTGTGCTA AGAAACCCCA GGAAAGAGTA 
ACTATTAGTA CTTCATTTTC AACTCTGAAA AAGCAATCGA AGTTTATGTA CAGTGTAAAC 
ACTGTGTTAA CTGGTCATAT TGCAAGCAAT ACCAGCATAG CCACTAGGAG ATCATTGAAC 
AGTCATTTAA GTTCTGATGA ACCAAAACCT CACATTAAGG AAAATCAAAA TGAACCAGAA 
TCAAATGCTA AATATTATTC CAACGGCCTG CAGAAACCAG TATTCCTGGA AAAGGAGAAT 
GATAATAAGG GAATTTCTTT ATGTAAAACT ATCTCAGACG GTCAAAACAC TTCAGAAGGT 
GCCAGGGCTC CAGACTATTT TGAGGAACGT GTTCCTGAAA CAAAAGATAG ATGCAAAGCT 
ACACTAAGCA TCAGGGAAAA GGTTGTGGCT ACAGCAAATT GTTTTGCAAG AAAACAGCTT 
GAAACAGATT ATCCAGAGGA TGCCTCAGCA CAACATGAGG CACTTCAGTT CCATGCTAAG 
CAATATGTGT CATGCTCAAT GCTAAATTCT AATGTGTCCA ATGACATGCA AATAAAGCCT 
CAAGTGTTAA CTCAGGCATG CGATTTTAGG AGCAGCGCAG AAAATCTTGT TGAAAAAACA 
AAAAATCAAT TAAGGCGGAA TGAAGATCAA TCATTTTCAT CAACTGGCAT AGGGAAAAAG 
CCAGCTAGTG GTGAAAAATT AGTAGGTGAC TGTAATGAGG GCTCATTCCT TCATGTGAAG 
CAAGAAATAA GTGCTGCTCA GGCATTGGTA GGTAATGGTT ACGAAACCAG AGACACTCCT 
GATGTCACTA GTGATCATAC AAGGAGTGTT ATTTCCAACC ATGGAAAGTC TCTGTGGGAG 
GAGGAGACAG CACTGACTGA ATTACCAGTC CCAGAAATTC TGAGTAAAAC TTTCAAGGGC 
TTTAAAACGG CATCTAATAA AAAAATACAT ATTTCAGATA AAAATATTGT AAAAGGTTGT 
GACTTGTTCA AAGAAATTGA GTTTGGCATT GGTGACGCTA AGCCCTGCAA TGAAGAAAAC 
AAAGAGCCCT TAAAGAAAAC TCCCTTTGAT GTTCCTGGGT ATGAAACAAA TTTAAAAGGA 
TTCAAAACTG CATCCAATAA AGAAATAAAT GTATCAGAAA ACAAATTTGC TAAAGGTAGA 
CTGCTGTTTA AAGACATCGA AGAGGAAAGC GGTCAAACTA CAGCTATAAT GGCAAAAGAT 
AATGAGCTGT TCATTAAGCC TGCATCCAGC AATAAGCCTG ACATATTTAA GAGTACATGT 
ATAAAAGGAA TGAATCCATC TGAGAACCTA GACAGTATGC CCCCCAAAAA AGGAGACGTG 
CAGCAGAGTG CAGGTTTAAA ACATTCAGAT CAAATTGACG TTTTTGGTTT CGATGATCCT 
CCTGCAAAAG GAAATGTTCA AACATTAGGC TATTTTAATA TGTCTGCTTC TGAAACAGAC 
TTCAAAGGAT TCAAAACCGC TTCCAATAAA GACATAATTA TTTCAGAAAG CACACTTGCT 
AAAGGTAAAC TTATTTTTGA AGATATTGAA GATACAAGAT ACAGTGAAAC AGGAAGGACC 
ATAGATTGTA AAGCAAAAGG TGGTGCGTTG TCCACCAGTA ACCTTGTAAT GCATAACACC 
ATGTGTAAAA ATGAACCAAG TACATCTGAG AACACAAATG ATAAAGCACC AAAAAGAGCA 
AAAGTACAAA CACCTATGGC AGAAAATAGT CATTTAAAAG AACCTTCTTC AGACAAAAAT 
GCAATGGATG CTGCAAATCA TCAAATGACA CTGAAAGCCT CCGCATACAG CCCAAAGGCT 
GCCTCTTCTG CACTGCCCAG GTACAAAAAG AAACCAATCT CTACCACTAA AACCTCTTTC 
CAGTTGAATG AACATTTGAC TGAAAGCCAG CAAGCTGAAA TCTCTGAACT TTCCTCTATT 
CTAGAAAATG CAGACAGTCA GTTTGATTTT ACTCAGTTTA GGAAAGTGCC TTCTGTGACT 
GAGAAACAAA ATTCAACTGA AGGTGGGAGC GAGTCCCAGA ACCTTAACAA CTCAGATGTC 
TGGAAGGACG TAGATTTTAA TGATAGTTTT GCAGCAGGAA GGGATCATTC TGAGGGAATG 
GAGGCAATTC CCTCCAGTCC TGGAATAAAA GAATTGCCTT CTGCAGGAGA ATGTTGCAGT 
AAAGAAACAC ATGATTCTAT GCCAGATTTT GTGACCTTGG CACCTAAACA AGGTTTTCTG 
GTTAAACAAA ATGAAAGACT CTTTGCAGGG TTTAATCTTG CTAGTGGCAA GCAAGTTAAT 
ATAGACAATG ATGTACTAAA AAAAGCTGCT GAACTGTTCA ATGATATAGA TAATGATAAA 
GAATTGCTCA GCCACGCCAA AGAAGAAAGC AGAAAAAGCA ATATAAAACA CTCTTCTAAA 
TTAATAAATA ATGAAAATTG TGGAAAGACA GAACACACAT CTGAGCATTT GGTATGTCAA 
AGTAATGTTT CATTACCATT CATAAAAACA TTGGGCAAGA ATGCTACAAT ACTAGTGAGT 
GGTAATGAAA AAAGCCAGAA TCAAGAATTA CAGCTGGATT GTTTAAAATG TGAAAGTACT 
GATGTAAAGC ATACACCACA AAAGGAAACA ATCAATGACA ACTCAAAGTG TAATGAATCC 
TCTCTTTCTG AGCTTTCCAT GAAAGGTTTT CAGACTGCCA GTGGGAGAAA CATTATGATG 
TCCGAGTCTT CAATCCAGAA AGCCAGAAAC ATATTTGCAG AGGAACATGA AGACAGTTTT 
ACATTGCGCT GTAATATTCA AAATACAATA CAAATACCAC AGCCTGTAAA TGAACCGACC 
CAATTCCCAT ATGTTAATTT GGGGCCAAAA CCCACAACGA CATCAGGATG GCAAGAAAAA 
AATATTCTGA GAAGAAGTAC AGAAAAAGGT TTCATGCCGG GTTTTTGCAC TGCCGGTGGT 
AAAAAGGTTT CTGTTTCCGA CAACTCTCTA GCTAAAGCAC ATAAACTTTT CCAGGAGGAA 
TGTACCTTTA GCAAGGAAGG GAAATTGGAT GAGGTGAAAC AAAATAAATT AATGAACTCT 
GAGCCATTGT CTCTTCTCAC TTGTGAGTCA GTCCTGAAAC AGAGTGACGG ATTCATTGAA 
GATATTTCAA CTTCTAGAAA TGCTTTGGAA ATCAGACCAG AGCTTTATCC GGAGGGCATG 
TGTAGTAATA GGGCATCTAA TGGAAGTGGA AACAATAGTG AATTTACAGC TGGGGAAGGC 
ATATCAATAA ATATCAACCA ATCATCGCTG CTTACAACAG GCAACGTTTT AAAAAATTTG 
CCGTCTGAAA GTAGTGGCCA TGATGTGTAT TCAGTAACTG AGCATCTAAG CACTGTGGTT 
AAAGTCAAAA GATACAATGA TTCAGGTCAT TTTGTAAATC AAAATTTAGC TGAATGTAAT 
GATAACCATG TCCTTAGCAC TCAAAAAAAT ACTGCAAATA TAAGCAACAG AAACGAGGAC 
TGTACATCCC TGGCACCTCT CTCTTTCAGC ACAGCCAGTG GGAAATCCGT CACAGTGTCT 
CATGATTCTC TTCAAAAAGC AAGACTGATG CTTTCTGAAG CAGCTAATGA TGTTACAGTA 
GATACTAGTA AGCAAGAGGC GGCTTATATT ACGCCAGCCA TTCGAAAAAC AGAAGCAGAA 
AAAGAGCAGA ACACTGTTGA TGATTCTGAT AGAGTTAATG CAAATACATT TTCATTTTCT 
ACTGCAAGCG GTAAAAAGGT CAACATAAGC GGAAATTCTT TAAAACAGGT CAGAGCTGTC 
TGCCTATCCT CTGACCCAAA AGAAACATCA GCGGCTCTGT TTAATGTTGA AAAAAGTGTT 
TTTAATGAAG ATGTTAAGGA TGTATCTTTG CTTCAGCCCA ATGTTACCAT GCCGAAGGCT 
GTGTCGTTTA GTACTGCAAG TGGCAAGACG GTGCAGTTGT CAGACGAGTC TTTGAAGAAA 
GCTAGGGTGA TTTTTTCTGA AATAGATACT TGTCCGTTGA TGCAACAGCA GACTAATGAA 
AGCACTGTTG AAGAGATAGT AATAGGAGGA GGAATGACAA AATCAAAACA GATGCCATTA 
ACAACAGAAA AAGTCGAAAC AACTAGAAAA AATAATGGTA CTTTTGGCTT TAATACCGCA 
AGCGGAAAGC AGGTCTCTGT GTCTGAAAGT GCGCTGCAGA AAGTCAAAGA TATCTTTCAA 
GAATTTGATG ATCCCGACAA TTATGAACAA AATAAATCAC TCGTAAGACT GCCTGTTTCA 
TCAAAAATAA AAGAATCAAC TCCAGGAACA AAAAGACTGG TGCAAACTGC AGGGTCCTCT 
TATAAAAATG ATAATTTGCA ATGTAAAGCA GGGAATCTTC GTACTTTTCA GGATAAGCAA 
GCTGGCAAGA AGTCACTTAC ATATTCAGAA GCAGCCATAA GCCCCATTAT AACAACTGTC 
CCTGGAAATC AATTAACGCC CATCCAGTTG GTAAAAGTCT CAACCAACAC ATCAGCATTG 
GCTAATAAAA CCAAACCCGA TTTATATGTC GCAACAGCCC AGAACACACC ACAAAATGAT 
TTTGAAATAG AAGCAGCAGA AAGTGCTAGA GCTTTCTTGG AAGATGATGA CCTGACAGAT 
ATGGGATCAG CTGCCCATGA AACATACTTG TATCTCAACC AGACCTCAAA TACAAGAACT 
GGGAAGAGAT CAAGAACAGA AGGGCCGACA CCTGGAGAGC CACCAATCAA AAGACGGCTT 
TTACCAGAAT TTGATGGCAC CACAGCCACT CGGCAACAAA CGGGGATTTT AAAGCCTTTG 
ATCAGCACTC CAGACATGAT GCGAGACAGG CGAAGGTTTT TGTACACCCT TCCCTTGAAG 
CCATCAACTT GTAATCCTGA AAGTGCTTTA CGTCAAGTCA CGTGCATTCC TACACAAGCC 
CATCTCCATT CTAAAGTCAA AATCTTCCAC CAGTCTCTAC CCATCAAGTC ACCAGATGTT 
GCATCTGATT CAACTTCTAA ATCGTATAGC CCCACTGCTG CAAAAGAGAC CATAAACTGC 
TCATCTGCAT CTAAAATACC TGCAAAGAAA TTTGTGCCTC CTTTTAAAAA GACTGTGGCG 
ACCTTGGCAG ACAACCAGTC AAACAGTGTA CAAAATGGCA GTTCGGATGG GCTTATCGAG 
AGCATTGTTT ATCCAAAAGA AGACAAAGTT GAAACCATAT GTTCTTCAAA AGATCAATTT 
GATGACTCAG ATATTTTACA AATGACTTCA AATCTTCGAT GTAGCAAAGA CCTGCAGGAA 
ATGAGAATTC GGAAAAAGCT AAGGCAAAAG ATTAAACCGC ATCCAGGCTC ACTGTATCGT 
CTGAAAATGT CACATGTGAA GAGAATTTCA CTTCAATCTG CAGTGGCAGA AAGGTGTCCC 
ACTCTGTATT CAAGGGAACA GTTATACAGA TATGGCATTG TGAAGAACCA TATTGGAGTC 
AGCAGCGAGA ACGCATTGTC CTTCCAGTTC CACTGTTCAA ATTATTTCAC AAAAGAACTT 
CTTCTTTCTG GAAATGGTGT GCAGTTGGCA GATGGAGGTT GGCTTATTCC CACTGAACAA 
GGCAACGCAG GCAAAGAAGA GATATACAGA GCGTTTTGTG ACACTCCTGG TGTGGATCCA 
AAATTAATTT CAGCTGAATG GGTTCATAAC CACTACCGCT GGATTGTATG GAAACTTGCT 
GCAATGGAAG TCAGGTTCCC AAAGACATTT GCTTGCAGAT GCCTTACACC AGAACGTGTG 
CTTTTACAGT TAAAGTACAG ATATGATGTG GAAATTGATA AGAGTCAGAG ATCCGCCATC 
AAAAAGATCA TGGAGAGAGA TGACTCCCCA GCTAAAACGC TGGTACTGTG CATTGCCAAG 
ATCATTTCCC AGGGCACTCG CCTGCCCAAT GCATGTAGTA ATAAAACCGA ACCCGCTGAC 
TCTAAGGAAA GTTCTGCAGT AATAGAGGTG ACAGATAGTT GGTATGGTAT CAAGGTTCTG 
CTTGATCCGT GTCTCACTGC TCTCTTACAC AAAGGAAGGC TTTTTATTGG TCAGAAGCTA 
ATTGTTCATG GAGCGGAACT TATTGGCTCA GATGATGCCT GCTCTCCCCT TGAAGCGCCA 
GAATCTCTTA TGTTGAAGAT TGCAGCTAAC AGCACTCGGC CTGTTCGATG GCATACCAAA 
CTTGGATATT TCAAGGATCC CAGACCATTC TGTCTCCATT TATCATCACT GTTAAGTGAA 
GGAGGTGTGG TGGGGTGTGT CGATGTAGTA ATACAAAGAA TCTATCCCAT GCAGTGGATG 
GAAAAAATGG CAAATGGACT GTATGTGTTT CGCAATGATC GAGCAGAGGA AAGAGAAGCA 
GAAAAGCATT CAGCAAATCA ACAAAAAAAG CTTGAAATGC TCTTTTCAAA AATACAAGCT 
GAATTTGAAC AGCGGGAAGT AACATGCAAC AGAAGAAAGG GACTCAGGCG GCGGTCATTA 
AATGCACAAC AAATGCAGAC CCTTCAGGAT GGGGCTGAAA TCTATGAAGC CATTCAGAAC 
GAATCTGACC CTGGGTATCT GGAGTCGTAC TTAAGTGCTG AGCAGCTGAA AGCCCTCAAT 
CACCACAGGC AACTTTTAAA CGATAAGAAG CAAGCACTGA TCCAAGCTGA ATTCAGAAAA 
GCCATTGAAT GCAGTGAGCA GGATGCAAAT GGCTGTACAA GGAGGGATGT CACTCCTGTA 
TGGAAATTAC GCATTGCAGA TTACAGAAAT TATGAAACAG ATGCAGCATA TATACTGAAC 
ATTTGGCGTC CGTTACCAGA TGTGCTTTCT CTTCTGAAAG AAGGTTGTCG CTATAAAATG 
TATCATTTGG CAGCATCCAC ATCAAAAGGA AAGTCATTAG CAGCTGATTT GCAGTTAACA 
GCTACAAAGA AAACTCGCTT CCAGCAACTT CAGCTATCAG AGAGCATTTT GGAGCAGATT 
TACAGCCCGA GAGAAGTGAC GGACTTCAGC AGGTTCCAGG AGCCTTTGTT TAGCGCCCCA 
TATGCAGAGG TTGATCTGGT GGGATTAATC ATCTCCATAT ACAAAAAAAC AGGTGCAGCT 
CCAGTGGTTT ATATATCAGA TGAATCTCAC AATATTGTGG CGCTAAAATT CTGGACAGAT 
CTAGGGCAGC TTGGGCTTGA GGAGATAACC AAGCCACGCA CATATATCTC TGCCAGTAAT 
CTTCGCTGGA GATCAGACTG TATCGAGGGG ATTCCTACAC TGTACGTTGG AGACTTAGCC 
AACATTTCTT CCAATCCCAA AGAAAGCCAT CTGCAGAGGG CAATTCAGAA ACTGAAACTT 
TCTGTACAGA ATGTCCAAGA TTTTTGGAAT TCATCACAAA CCGCACTGAT GAAAACCTTG 
CAGATAAATA GCACAGACAC AACGGAGTGC AGTAAGAACC CAACGACACC AACATGGAAG 
TCAGATGTTT CTGCTAGAAG TGGTTACCTA ACCCCCTTGC ACCATAGTGG TAAAAGGTTA 
TTAAATTCTG TCCATACATC AGATCCTCAG ACAGAAAATC CTGGATGTAG TAAAGAAATA 
CAACTAAAAA CCTGCAAAAA GAGGAAAGCT CTGGACTTTC TCAACCGCAT TCCTTCACCT 
CCACCTGTAA CACCAGTACG GCCCTTTGTA TCCCCATCTT TGCAGAAGGC ATTCCGACCC 
CCACGGAGCT GTAGCGTCCA GAAGTTGGGC CCGGAAACCA AGGGTAATAC TGAGAATGTC 
CAGGGAACAA CCCCAGAATG CACTAAAGAC CTAGCAAAAT TAGAGGGTGA ATTTGTGGCT 
GATGAAGAAC TCGCCATGAT CAATACCCAA GCTTTGCTTT TAGGTCTGGA GGAAGAAAAA 
AAGAAAACCG AGCAGAAAAC CAGCAGAACA GCAGGTAAAA TGACTGCCCA TGAGTCTCCA 
ATAGAAAATG CTTCCCCCGT TCCTGCCCAG GAACAACAAA CTGAGGAAGC ATTGAACATT 
CCAGTCGGTA ACTCAGAGAA ATCTTACCTG TGCCTAAGAA AAAGGAAACG GAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947219.1
CDS	306..9962
Translation	MAAPQLGKSVFYDLFSTHCSHSDLGPISLNWFEELTAEALPYKSRTCEDHECSLDDLHENNIKTPKLKSSIYSQLDSTPVIFKERLLSPLFASSLTELDKRQNATDNGNNVRLEKSNCTQMQHQASEVFSPSSRCLNESPAVIKEIFKTPLRNKYLHKTPQCDWKPDICSSLFCTPKLMKNQTGCIKESLGAEVDPEMSWSSSLATPPSPTVIIAHANDQPSGNKHAAIVQSLFPNCENMEANNLPPPETGTQNEDRQICAGLEKPGSCSASKTVPEASAISSRAAWKKTVANTVKDEEVSRTVENALEGMEDVLSIFFASEKTPGLRKLKNSTQARRKVESIKSQKCQVSLFKAEEGESILVPLDEHHKCDHEMLMKPEQDYKMNAVNQKIAKETTPYEWSQLNICELDITQSQDSTLHAANLCCEDITVNKSDTCNITEAEKQEEVNEPSTDNVLSNKVGKESTLNVNCINADLNNMASSSNNSVHSLNLSQCEKMDSSEISNSEVGCITKLTTHPAKITTGELSSVDDCAKKPQERVTISTSFSTLKKQSKFMYSVNTVLTGHIASNTSIATRRSLNSHLSSDEPKPHIKENQNEPESNAKYYSNGLQKPVFLEKENDNKGISLCKTISDGQNTSEGARAPDYFEERVPETKDRCKATLSIREKVVATANCFARKQLETDYPEDASAQHEALQFHAKQYVSCSMLNSNVSNDMQIKPQVLTQACDFRSSAENLVEKTKNQLRRNEDQSFSSTGIGKKPASGEKLVGDCNEGSFLHVKQEISAAQALVGNGYETRDTPDVTSDHTRSVISNHGKSLWEEETALTELPVPEILSKTFKGFKTASNKKIHISDKNIVKGCDLFKEIEFGIGDAKPCNEENKEPLKKTPFDVPGYETNLKGFKTASNKEINVSENKFAKGRLLFKDIEEESGQTTAIMAKDNELFIKPASSNKPDIFKSTCIKGMNPSENLDSMPPKKGDVQQSAGLKHSDQIDVFGFDDPPAKGNVQTLGYFNMSASETDFKGFKTASNKDIIISESTLAKGKLIFEDIEDTRYSETGRTIDCKAKGGALSTSNLVMHNTMCKNEPSTSENTNDKAPKRAKVQTPMAENSHLKEPSSDKNAMDAANHQMTLKASAYSPKAASSALPRYKKKPISTTKTSFQLNEHLTESQQAEISELSSILENADSQFDFTQFRKVPSVTEKQNSTEGGSESQNLNNSDVWKDVDFNDSFAAGRDHSEGMEAIPSSPGIKELPSAGECCSKETHDSMPDFVTLAPKQGFLVKQNERLFAGFNLASGKQVNIDNDVLKKAAELFNDIDNDKELLSHAKEESRKSNIKHSSKLINNENCGKTEHTSEHLVCQSNVSLPFIKTLGKNATILVSGNEKSQNQELQLDCLKCESTDVKHTPQKETINDNSKCNESSLSELSMKGFQTASGRNIMMSESSIQKARNIFAEEHEDSFTLRCNIQNTIQIPQPVNEPTQFPYVNLGPKPTTTSGWQEKNILRRSTEKGFMPGFCTAGGKKVSVSDNSLAKAHKLFQEECTFSKEGKLDEVKQNKLMNSEPLSLLTCESVLKQSDGFIEDISTSRNALEIRPELYPEGMCSNRASNGSGNNSEFTAGEGISININQSSLLTTGNVLKNLPSESSGHDVYSVTEHLSTVVKVKRYNDSGHFVNQNLAECNDNHVLSTQKNTANISNRNEDCTSLAPLSFSTASGKSVTVSHDSLQKARLMLSEAANDVTVDTSKQEAAYITPAIRKTEAEKEQNTVDDSDRVNANTFSFSTASGKKVNISGNSLKQVRAVCLSSDPKETSAALFNVEKSVFNEDVKDVSLLQPNVTMPKAVSFSTASGKTVQLSDESLKKARVIFSEIDTCPLMQQQTNESTVEEIVIGGGMTKSKQMPLTTEKVETTRKNNGTFGFNTASGKQVSVSESALQKVKDIFQEFDDPDNYEQNKSLVRLPVSSKIKESTPGTKRLVQTAGSSYKNDNLQCKAGNLRTFQDKQAGKKSLTYSEAAISPIITTVPGNQLTPIQLVKVSTNTSALANKTKPDLYVATAQNTPQNDFEIEAAESARAFLEDDDLTDMGSAAHETYLYLNQTSNTRTGKRSRTEGPTPGEPPIKRRLLPEFDGTTATRQQTGILKPLISTPDMMRDRRRFLYTLPLKPSTCNPESALRQVTCIPTQAHLHSKVKIFHQSLPIKSPDVASDSTSKSYSPTAAKETINCSSASKIPAKKFVPPFKKTVATLADNQSNSVQNGSSDGLIESIVYPKEDKVETICSSKDQFDDSDILQMTSNLRCSKDLQEMRIRKKLRQKIKPHPGSLYRLKMSHVKRISLQSAVAERCPTLYSREQLYRYGIVKNHIGVSSENALSFQFHCSNYFTKELLLSGNGVQLADGGWLIPTEQGNAGKEEIYRAFCDTPGVDPKLISAEWVHNHYRWIVWKLAAMEVRFPKTFACRCLTPERVLLQLKYRYDVEIDKSQRSAIKKIMERDDSPAKTLVLCIAKIISQGTRLPNACSNKTEPADSKESSAVIEVTDSWYGIKVLLDPCLTALLHKGRLFIGQKLIVHGAELIGSDDACSPLEAPESLMLKIAANSTRPVRWHTKLGYFKDPRPFCLHLSSLLSEGGVVGCVDVVIQRIYPMQWMEKMANGLYVFRNDRAEEREAEKHSANQQKKLEMLFSKIQAEFEQREVTCNRRKGLRRRSLNAQQMQTLQDGAEIYEAIQNESDPGYLESYLSAEQLKALNHHRQLLNDKKQALIQAEFRKAIECSEQDANGCTRRDVTPVWKLRIADYRNYETDAAYILNIWRPLPDVLSLLKEGCRYKMYHLAASTSKGKSLAADLQLTATKKTRFQQLQLSESILEQIYSPREVTDFSRFQEPLFSAPYAEVDLVGLIISIYKKTGAAPVVYISDESHNIVALKFWTDLGQLGLEEITKPRTYISASNLRWRSDCIEGIPTLYVGDLANISSNPKESHLQRAIQKLKLSVQNVQDFWNSSQTALMKTLQINSTDTTECSKNPTTPTWKSDVSARSGYLTPLHHSGKRLLNSVHTSDPQTENPGCSKEIQLKTCKKRKALDFLNRIPSPPPVTPVRPFVSPSLQKAFRPPRSCSVQKLGPETKGNTENVQGTTPECTKDLAKLEGEFVADEELAMINTQALLLGLEEEKKKTEQKTSRTAGKMTAHESPIENASPVPAQEQQTEEALNIPVGNSEKSYLCLRKRKRK

Target ORF information:

RefSeq Version	XM_018091730.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis breast cancer 2 (brca2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091730.1

ORF Insert Sequence:

ATGGCTGCAC CGCAACTTGG AAAATCTGTC TTCTATGATC TGTTTAGCAC GCATTGCTCT 
CACTCAGATT TAGGGCCTAT CAGTCTAAAC TGGTTTGAAG AGCTCACTGC TGAAGCCTTG 
CCTTACAAGT CAAGAACATG TGAAGATCAT GAATGCAGTT TGGATGATCT CCATGAAAAC 
AACATCAAAA CTCCTAAACT GAAGTCGTCA ATTTATAGCC AATTAGATTC CACTCCAGTC 
ATCTTTAAGG AGCGTTTACT ATCACCTCTT TTTGCTTCTT CTTTGACAGA GCTGGATAAA 
AGGCAGAATG CAACAGATAA TGGAAACAAT GTTAGACTTG AGAAAAGTAA CTGTACCCAA 
ATGCAGCATC AAGCAAGTGA AGTTTTCAGT CCCTCAAGTC GATGCCTCAA CGAAAGTCCT 
GCTGTTATCA AAGAAATATT CAAGACACCA TTACGAAATA AGTACCTACA TAAAACACCA 
CAGTGTGATT GGAAACCTGA CATTTGCAGC AGTCTGTTTT GTACTCCAAA GCTTATGAAG 
AACCAAACTG GATGTATTAA AGAAAGTCTA GGTGCAGAGG TGGATCCAGA GATGTCATGG 
TCTAGTTCAC TAGCTACACC TCCTTCCCCA ACTGTGATAA TTGCACATGC TAATGATCAG 
CCCAGTGGAA ATAAACATGC TGCAATTGTA CAGAGCCTTT TCCCAAATTG TGAGAATATG 
GAAGCGAATA ATTTGCCACC ACCTGAGACG GGCACACAAA ATGAAGACCG TCAAATATGT 
GCAGGACTAG AAAAGCCAGG TTCATGCAGT GCGAGCAAAA CTGTACCAGA AGCGTCTGCC 
ATTTCCTCAA GGGCAGCCTG GAAGAAAACT GTTGCAAATA CAGTGAAGGA CGAGGAGGTT 
TCCAGAACTG TGGAAAATGC TCTGGAAGGA ATGGAAGATG TCCTCTCAAT CTTTTTTGCA 
AGTGAAAAGA CTCCTGGTCT CAGAAAGCTG AAAAATTCTA CCCAAGCTAG GAGAAAAGTG 
GAATCTATTA AGTCTCAGAA GTGCCAAGTT TCATTATTTA AAGCAGAAGA AGGAGAAAGC 
ATTTTGGTCC CTTTAGATGA GCATCATAAA TGTGATCATG AGATGCTTAT GAAACCTGAA 
CAAGACTACA AAATGAATGC TGTTAACCAA AAAATAGCCA AAGAAACTAC TCCATATGAA 
TGGTCACAGC TAAATATATG TGAGTTGGAT ATAACTCAAT CACAGGATAG CACTTTGCAT 
GCAGCAAATC TATGTTGTGA AGATATCACT GTCAACAAAA GTGATACATG TAATATTACT 
GAAGCCGAAA AGCAGGAGGA AGTCAATGAG CCAAGCACCG ACAATGTACT GTCTAATAAA 
GTTGGCAAAG AAAGCACTTT AAATGTGAAT TGTATTAATG CTGATTTGAA TAACATGGCT 
TCATCCTCAA ATAACTCTGT TCACTCACTG AATCTTTCTC AATGTGAAAA AATGGATTCT 
TCTGAAATTT CTAACAGTGA AGTTGGTTGT ATAACAAAAC TAACAACCCA CCCAGCAAAA 
ATAACAACTG GTGAATTAAG TTCAGTAGAT GACTGTGCTA AGAAACCCCA GGAAAGAGTA 
ACTATTAGTA CTTCATTTTC AACTCTGAAA AAGCAATCGA AGTTTATGTA CAGTGTAAAC 
ACTGTGTTAA CTGGTCATAT TGCAAGCAAT ACCAGCATAG CCACTAGGAG ATCATTGAAC 
AGTCATTTAA GTTCTGATGA ACCAAAACCT CACATTAAGG AAAATCAAAA TGAACCAGAA 
TCAAATGCTA AATATTATTC CAACGGCCTG CAGAAACCAG TATTCCTGGA AAAGGAGAAT 
GATAATAAGG GAATTTCTTT ATGTAAAACT ATCTCAGACG GTCAAAACAC TTCAGAAGGT 
GCCAGGGCTC CAGACTATTT TGAGGAACGT GTTCCTGAAA CAAAAGATAG ATGCAAAGCT 
ACACTAAGCA TCAGGGAAAA GGTTGTGGCT ACAGCAAATT GTTTTGCAAG AAAACAGCTT 
GAAACAGATT ATCCAGAGGA TGCCTCAGCA CAACATGAGG CACTTCAGTT CCATGCTAAG 
CAATATGTGT CATGCTCAAT GCTAAATTCT AATGTGTCCA ATGACATGCA AATAAAGCCT 
CAAGTGTTAA CTCAGGCATG CGATTTTAGG AGCAGCGCAG AAAATCTTGT TGAAAAAACA 
AAAAATCAAT TAAGGCGGAA TGAAGATCAA TCATTTTCAT CAACTGGCAT AGGGAAAAAG 
CCAGCTAGTG GTGAAAAATT AGTAGGTGAC TGTAATGAGG GCTCATTCCT TCATGTGAAG 
CAAGAAATAA GTGCTGCTCA GGCATTGGTA GGTAATGGTT ACGAAACCAG AGACACTCCT 
GATGTCACTA GTGATCATAC AAGGAGTGTT ATTTCCAACC ATGGAAAGTC TCTGTGGGAG 
GAGGAGACAG CACTGACTGA ATTACCAGTC CCAGAAATTC TGAGTAAAAC TTTCAAGGGC 
TTTAAAACGG CATCTAATAA AAAAATACAT ATTTCAGATA AAAATATTGT AAAAGGTTGT 
GACTTGTTCA AAGAAATTGA GTTTGGCATT GGTGACGCTA AGCCCTGCAA TGAAGAAAAC 
AAAGAGCCCT TAAAGAAAAC TCCCTTTGAT GTTCCTGGGT ATGAAACAAA TTTAAAAGGA 
TTCAAAACTG CATCCAATAA AGAAATAAAT GTATCAGAAA ACAAATTTGC TAAAGGTAGA 
CTGCTGTTTA AAGACATCGA AGAGGAAAGC GGTCAAACTA CAGCTATAAT GGCAAAAGAT 
AATGAGCTGT TCATTAAGCC TGCATCCAGC AATAAGCCTG ACATATTTAA GAGTACATGT 
ATAAAAGGAA TGAATCCATC TGAGAACCTA GACAGTATGC CCCCCAAAAA AGGAGACGTG 
CAGCAGAGTG CAGGTTTAAA ACATTCAGAT CAAATTGACG TTTTTGGTTT CGATGATCCT 
CCTGCAAAAG GAAATGTTCA AACATTAGGC TATTTTAATA TGTCTGCTTC TGAAACAGAC 
TTCAAAGGAT TCAAAACCGC TTCCAATAAA GACATAATTA TTTCAGAAAG CACACTTGCT 
AAAGGTAAAC TTATTTTTGA AGATATTGAA GATACAAGAT ACAGTGAAAC AGGAAGGACC 
ATAGATTGTA AAGCAAAAGG TGGTGCGTTG TCCACCAGTA ACCTTGTAAT GCATAACACC 
ATGTGTAAAA ATGAACCAAG TACATCTGAG AACACAAATG ATAAAGCACC AAAAAGAGCA 
AAAGTACAAA CACCTATGGC AGAAAATAGT CATTTAAAAG AACCTTCTTC AGACAAAAAT 
GCAATGGATG CTGCAAATCA TCAAATGACA CTGAAAGCCT CCGCATACAG CCCAAAGGCT 
GCCTCTTCTG CACTGCCCAG GTACAAAAAG AAACCAATCT CTACCACTAA AACCTCTTTC 
CAGTTGAATG AACATTTGAC TGAAAGCCAG CAAGCTGAAA TCTCTGAACT TTCCTCTATT 
CTAGAAAATG CAGACAGTCA GTTTGATTTT ACTCAGTTTA GGAAAGTGCC TTCTGTGACT 
GAGAAACAAA ATTCAACTGA AGGTGGGAGC GAGTCCCAGA ACCTTAACAA CTCAGATGTC 
TGGAAGGACG TAGATTTTAA TGATAGTTTT GCAGCAGGAA GGGATCATTC TGAGGGAATG 
GAGGCAATTC CCTCCAGTCC TGGAATAAAA GAATTGCCTT CTGCAGGAGA ATGTTGCAGT 
AAAGAAACAC ATGATTCTAT GCCAGATTTT GTGACCTTGG CACCTAAACA AGGTTTTCTG 
GTTAAACAAA ATGAAAGACT CTTTGCAGGG TTTAATCTTG CTAGTGGCAA GCAAGTTAAT 
ATAGACAATG ATGTACTAAA AAAAGCTGCT GAACTGTTCA ATGATATAGA TAATGATAAA 
GAATTGCTCA GCCACGCCAA AGAAGAAAGC AGAAAAAGCA ATATAAAACA CTCTTCTAAA 
TTAATAAATA ATGAAAATTG TGGAAAGACA GAACACACAT CTGAGCATTT GGTATGTCAA 
AGTAATGTTT CATTACCATT CATAAAAACA TTGGGCAAGA ATGCTACAAT ACTAGTGAGT 
GGTAATGAAA AAAGCCAGAA TCAAGAATTA CAGCTGGATT GTTTAAAATG TGAAAGTACT 
GATGTAAAGC ATACACCACA AAAGGAAACA ATCAATGACA ACTCAAAGTG TAATGAATCC 
TCTCTTTCTG AGCTTTCCAT GAAAGGTTTT CAGACTGCCA GTGGGAGAAA CATTATGATG 
TCCGAGTCTT CAATCCAGAA AGCCAGAAAC ATATTTGCAG AGGAACATGA AGACAGTTTT 
ACATTGCGCT GTAATATTCA AAATACAATA CAAATACCAC AGCCTGTAAA TGAACCGACC 
CAATTCCCAT ATGTTAATTT GGGGCCAAAA CCCACAACGA CATCAGGATG GCAAGAAAAA 
AATATTCTGA GAAGAAGTAC AGAAAAAGGT TTCATGCCGG GTTTTTGCAC TGCCGGTGGT 
AAAAAGGTTT CTGTTTCCGA CAACTCTCTA GCTAAAGCAC ATAAACTTTT CCAGGAGGAA 
TGTACCTTTA GCAAGGAAGG GAAATTGGAT GAGGTGAAAC AAAATAAATT AATGAACTCT 
GAGCCATTGT CTCTTCTCAC TTGTGAGTCA GTCCTGAAAC AGAGTGACGG ATTCATTGAA 
GATATTTCAA CTTCTAGAAA TGCTTTGGAA ATCAGACCAG AGCTTTATCC GGAGGGCATG 
TGTAGTAATA GGGCATCTAA TGGAAGTGGA AACAATAGTG AATTTACAGC TGGGGAAGGC 
ATATCAATAA ATATCAACCA ATCATCGCTG CTTACAACAG GCAACGTTTT AAAAAATTTG 
CCGTCTGAAA GTAGTGGCCA TGATGTGTAT TCAGTAACTG AGCATCTAAG CACTGTGGTT 
AAAGTCAAAA GATACAATGA TTCAGGTCAT TTTGTAAATC AAAATTTAGC TGAATGTAAT 
GATAACCATG TCCTTAGCAC TCAAAAAAAT ACTGCAAATA TAAGCAACAG AAACGAGGAC 
TGTACATCCC TGGCACCTCT CTCTTTCAGC ACAGCCAGTG GGAAATCCGT CACAGTGTCT 
CATGATTCTC TTCAAAAAGC AAGACTGATG CTTTCTGAAG CAGCTAATGA TGTTACAGTA 
GATACTAGTA AGCAAGAGGC GGCTTATATT ACGCCAGCCA TTCGAAAAAC AGAAGCAGAA 
AAAGAGCAGA ACACTGTTGA TGATTCTGAT AGAGTTAATG CAAATACATT TTCATTTTCT 
ACTGCAAGCG GTAAAAAGGT CAACATAAGC GGAAATTCTT TAAAACAGGT CAGAGCTGTC 
TGCCTATCCT CTGACCCAAA AGAAACATCA GCGGCTCTGT TTAATGTTGA AAAAAGTGTT 
TTTAATGAAG ATGTTAAGGA TGTATCTTTG CTTCAGCCCA ATGTTACCAT GCCGAAGGCT 
GTGTCGTTTA GTACTGCAAG TGGCAAGACG GTGCAGTTGT CAGACGAGTC TTTGAAGAAA 
GCTAGGGTGA TTTTTTCTGA AATAGATACT TGTCCGTTGA TGCAACAGCA GACTAATGAA 
AGCACTGTTG AAGAGATAGT AATAGGAGGA GGAATGACAA AATCAAAACA GATGCCATTA 
ACAACAGAAA AAGTCGAAAC AACTAGAAAA AATAATGGTA CTTTTGGCTT TAATACCGCA 
AGCGGAAAGC AGGTCTCTGT GTCTGAAAGT GCGCTGCAGA AAGTCAAAGA TATCTTTCAA 
GAATTTGATG ATCCCGACAA TTATGAACAA AATAAATCAC TCGTAAGACT GCCTGTTTCA 
TCAAAAATAA AAGAATCAAC TCCAGGAACA AAAAGACTGG TGCAAACTGC AGGGTCCTCT 
TATAAAAATG ATAATTTGCA ATGTAAAGCA GGGAATCTTC GTACTTTTCA GGATAAGCAA 
GCTGGCAAGA AGTCACTTAC ATATTCAGAA GCAGCCATAA GCCCCATTAT AACAACTGTC 
CCTGGAAATC AATTAACGCC CATCCAGTTG GTAAAAGTCT CAACCAACAC ATCAGCATTG 
GCTAATAAAA CCAAACCCGA TTTATATGTC GCAACAGCCC AGAACACACC ACAAAATGAT 
TTTGAAATAG AAGCAGCAGA AAGTGCTAGA GCTTTCTTGG AAGATGATGA CCTGACAGAT 
ATGGGATCAG CTGCCCATGA AACATACTTG TATCTCAACC AGACCTCAAA TACAAGAACT 
GGGAAGAGAT CAAGAACAGA AGGGCCGACA CCTGGAGAGC CACCAATCAA AAGACGGCTT 
TTACCAGAAT TTGATGGCAC CACAGCCACT CGGCAACAAA CGGGGATTTT AAAGCCTTTG 
ATCAGCACTC CAGACATGAT GCGAGACAGG CGAAGGTTTT TGTACACCCT TCCCTTGAAG 
CCATCAACTT GTAATCCTGA AAGTGCTTTA CGTCAAGTCA CGTGCATTCC TACACAAGCC 
CATCTCCATT CTAAAGTCAA AATCTTCCAC CAGTCTCTAC CCATCAAGTC ACCAGATGTT 
GCATCTGATT CAACTTCTAA ATCGTATAGC CCCACTGCTG CAAAAGAGAC CATAAACTGC 
TCATCTGCAT CTAAAATACC TGCAAAGAAA TTTGTGCCTC CTTTTAAAAA GACTGTGGCG 
ACCTTGGCAG ACAACCAGTC AAACAGTGTA CAAAATGGCA GTTCGGATGG GCTTATCGAG 
AGCATTGTTT ATCCAAAAGA AGACAAAGTT GAAACCATAT GTTCTTCAAA AGATCAATTT 
GATGACTCAG ATATTTTACA AATGACTTCA AATCTTCGAT GTAGCAAAGA CCTGCAGGAA 
ATGAGAATTC GGAAAAAGCT AAGGCAAAAG ATTAAACCGC ATCCAGGCTC ACTGTATCGT 
CTGAAAATGT CACATGTGAA GAGAATTTCA CTTCAATCTG CAGTGGCAGA AAGGTGTCCC 
ACTCTGTATT CAAGGGAACA GTTATACAGA TATGGCATTG TGAAGAACCA TATTGGAGTC 
AGCAGCGAGA ACGCATTGTC CTTCCAGTTC CACTGTTCAA ATTATTTCAC AAAAGAACTT 
CTTCTTTCTG GAAATGGTGT GCAGTTGGCA GATGGAGGTT GGCTTATTCC CACTGAACAA 
GGCAACGCAG GCAAAGAAGA GATATACAGA GCGTTTTGTG ACACTCCTGG TGTGGATCCA 
AAATTAATTT CAGCTGAATG GGTTCATAAC CACTACCGCT GGATTGTATG GAAACTTGCT 
GCAATGGAAG TCAGGTTCCC AAAGACATTT GCTTGCAGAT GCCTTACACC AGAACGTGTG 
CTTTTACAGT TAAAGTACAG ATATGATGTG GAAATTGATA AGAGTCAGAG ATCCGCCATC 
AAAAAGATCA TGGAGAGAGA TGACTCCCCA GCTAAAACGC TGGTACTGTG CATTGCCAAG 
ATCATTTCCC AGGGCACTCG CCTGCCCAAT GCATGTAGTA ATAAAACCGA ACCCGCTGAC 
TCTAAGGAAA GTTCTGCAGT AATAGAGGTG ACAGATAGTT GGTATGGTAT CAAGGTTCTG 
CTTGATCCGT GTCTCACTGC TCTCTTACAC AAAGGAAGGC TTTTTATTGG TCAGAAGCTA 
ATTGTTCATG GAGCGGAACT TATTGGCTCA GATGATGCCT GCTCTCCCCT TGAAGCGCCA 
GAATCTCTTA TGTTGAAGAT TGCAGCTAAC AGCACTCGGC CTGTTCGATG GCATACCAAA 
CTTGGATATT TCAAGGATCC CAGACCATTC TGTCTCCATT TATCATCACT GTTAAGTGAA 
GGAGGTGTGG TGGGGTGTGT CGATGTAGTA ATACAAAGAA TCTATCCCAT GCAGTGGATG 
GAAAAAATGG CAAATGGACT GTATGTGTTT CGCAATGATC GAGCAGAGGA AAGAGAAGCA 
GAAAAGCATT CAGCAAATCA ACAAAAAAAG CTTGAAATGC TCTTTTCAAA AATACAAGCT 
GAATTTGAAC AGCGGGAAGT AACATGCAAC AGAAGAAAGG GACTCAGGCG GCGGTCATTA 
AATGCACAAC AAATGCAGAC CCTTCAGGAT GGGGCTGAAA TCTATGAAGC CATTCAGAAC 
GAATCTGACC CTGGGTATCT GGAGTCGTAC TTAAGTGCTG AGCAGCTGAA AGCCCTCAAT 
CACCACAGGC AACTTTTAAA CGATAAGAAG CAAGCACTGA TCCAAGCTGA ATTCAGAAAA 
GCCATTGAAT GCAGTGAGCA GGATGCAAAT GGCTGTACAA GGAGGGATGT CACTCCTGTA 
TGGAAATTAC GCATTGCAGA TTACAGAAAT TATGAAACAG ATGCAGCATA TATACTGAAC 
ATTTGGCGTC CGTTACCAGA TGTGCTTTCT CTTCTGAAAG AAGGTTGTCG CTATAAAATG 
TATCATTTGG CAGCATCCAC ATCAAAAGGA AAGTCATTAG CAGCTGATTT GCAGTTAACA 
GCTACAAAGA AAACTCGCTT CCAGCAACTT CAGCTATCAG AGAGCATTTT GGAGCAGATT 
TACAGCCCGA GAGAAGTGAC GGACTTCAGC AGGTTCCAGG AGCCTTTGTT TAGCGCCCCA 
TATGCAGAGG TTGATCTGGT GGGATTAATC ATCTCCATAT ACAAAAAAAC AGGTGCAGCT 
CCAGTGGTTT ATATATCAGA TGAATCTCAC AATATTGTGG CGCTAAAATT CTGGACAGAT 
CTAGGGCAGC TTGGGCTTGA GGAGATAACC AAGCCACGCA CATATATCTC TGCCAGTAAT 
CTTCGCTGGA GATCAGACTG TATCGAGGGG ATTCCTACAC TGTACGTTGG AGACTTAGCC 
AACATTTCTT CCAATCCCAA AGAAAGCCAT CTGCAGAGGG CAATTCAGAA ACTGAAACTT 
TCTGTACAGA ATGTCCAAGA TTTTTGGAAT TCATCACAAA CCGCACTGAT GAAAACCTTG 
CAGATAAATA GCACAGACAC AACGGAGTGC AGTAAGAACC CAACGACACC AACATGGAAG 
TCAGATGTTT CTGCTAGAAG TGGTTACCTA ACCCCCTTGC ACCATAGTGG TAAAAGGTTA 
TTAAATTCTG TCCATACATC AGATCCTCAG ACAGAAAATC CTGGATGTAG TAAAGAAATA 
CAACTAAAAA CCTGCAAAAA GAGGAAAGCT CTGGACTTTC TCAACCGCAT TCCTTCACCT 
CCACCTGTAA CACCAGTACG GCCCTTTGTA TCCCCATCTT TGCAGAAGGC ATTCCGACCC 
CCACGGAGCT GTAGCGTCCA GAAGTTGGGC CCGGAAACCA AGGGTAATAC TGAGAATGTC 
CAGGGAACAA CCCCAGAATG CACTAAAGAC CTAGCAAAAT TAGAGGGTGA ATTTGTGGCT 
GATGAAGAAC TCGCCATGAT CAATACCCAA GCTTTGCTTT TAGGTCTGGA GGAAGAAAAA 
AAGAAAACCG AGCAGAAAAC CAGCAGAACA GCAGGTAAAA TGACTGCCCA TGAGTCTCCA 
ATAGAAAATG CTTCCCCCGT TCCTGCCCAG GAACAACAAA CTGAGGAAGC ATTGAACATT 
CCAGTCGGTA ACTCAGAGAA ATCTTACCTG TGCCTAAGAA AAAGGAAACG GAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

brca2 ( XM_031897235.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031753095.1 Xenopus tropicalis BRCA2, DNA repair associated (brca2), transcript variant X2, mRNA.

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CloneID	OXa52805
Clone ID Related Accession (Same CDS sequence)	XM_031897235.1 , XM_031897234.1
Accession Version	XM_031897235.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	breast cancer type 2 susceptibility protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAC CGCAACTTGG AAAATCTGTC TTCTATGATC TGTTTAGCAC GCATTGCTCT 
CACTCAGATT TAGGGCCTAT CAGTCTAAAC TGGTTTGAAG AGCTCACTGC TGAAGCCTTG 
CCTTACAAGT CAAGAACATG TGAAGATCAT GAATGCAGTT TGGATGATCT CCATGAAAAC 
AACATCAAAA CTCCTAAACT GAAGTCGTCA ATTTATAGCC AATTAGATTC CACTCCAGTC 
ATCTTTAAGG AGCGTTTACT ATCACCTCTT TTTGCTTCTT CTTTGACAGA GCTGGATAAA 
AGGCAGAATG CAACAGATAA TGGAAACAAT GTTAGACTTG AGAAAAGTAA CTGTACCCAA 
ATGCAGCATC AAGCAAGTGA AGTTTTCAGT CCCTCAAGTC GATGCCTCAA CGAAAGTCCT 
GCTGTTATCA AAGAAATATT CAAGACACCA TTACGAAATA AGTACCTACA TAAAACACCA 
CAGTGTGATT GGAAACCTGA CATTTGCAGC AGTCTGTTTT GTACTCCAAA GCTTATGAAG 
AACCAAACTG GATGTATTAA AGAAAGTCTA GGTGCAGAGG TGGATCCAGA GATGTCATGG 
TCTAGTTCAC TAGCTACACC TCCTTCCCCA ACTGTGATAA TTGCACATGC TAATGATCAG 
CCCAGTGGAA ATAAACATGC TGCAATTGTA CAGAGCCTTT TCCCAAATTG TGAGAATATG 
GAAGCGAATA ATTTGCCACC ACCTGAGACG GGCACACAAA ATGAAGACCG TCAAATATGT 
GGTATGGGAT CTGTTATCCA GAAAATTCCG GAAAGGTCAT CTTCCATAGA CTCCATTATA 
ACAGGACTAG AAAAGCCAGG TTCATGCAGT GCGAGCAAAA CTGTACCAGA AGCGTCTGCC 
ATTTCCTCAA GGGCAGCCTG GAAGAAAACT GTTGCAAATA CAGTGAAGGA CGAGGAGGTT 
TCCAGAACTG TGGAAAATGC TCTGGAAGGA ATGGAAGATG TCCTCTCAAT CTTTTTTGCA 
AGTGAAAAGA CTCCTGGTCT CAGAAAGCTG AAAAATTCTA CCCAAGCTAG GAGAAAAGTG 
GAATCTATTA AGTCTCAGAA GTGCCAAGTT TCATTATTTA AAGCAGAAGA AGGAGAAAGC 
ATTTTGGTCC CTTTAGATGA GCATCATAAA TGTGATCATG AGATGCTTAT GAAACCTGAA 
CAAGACTACA AAATGAATGC TGTTAACCAA AAAATAGCCA AAGAAACTAC TCCATATGAA 
TGGTCACAGC TAAATATATG TGAGTTGGAT ATAACTCAAT CACAGGATAG CACTTTGCAT 
GCAGCAAATC TATGTTGTGA AGATATCACT GTCAACAAAA GTGATACATG TAATATTACT 
GAAGCCGAAA AGCAGGAGGA AGTCAATGAG CCAAGCACCG ACAATGTACT GTCTAATAAA 
GTTGGCAAAG AAAGCACTTT AAATGTGAAT TGTATTAATG CTGATTTGAA TAACATGGCT 
TCATCCTCAA ATAACTCTGT TCACTCACTG AATCTTTCTC AATGTGAAAA AATGGATTCT 
TCTGAAATTT CTAACAGTGA AGTTGGTTGT ATAACAAAAC TAACAACCCA CCCAGCAAAA 
ATAACAACTG GTGAATTAAG TTCAGTAGAT GACTGTGCTA AGAAACCCCA GGAAAGAGTA 
ACTATTAGTA CTTCATTTTC AACTCTGAAA AAGCAATCGA AGTTTATGTA CAGTGTAAAC 
ACTGTGTTAA CTGGTCATAT TGCAAGCAAT ACCAGCATAG CCACTAGGAG ATCATTGAAC 
AGTCATTTAA GTTCTGATGA ACCAAAACCT CACATTAAGG AAAATCAAAA TGAACCAGAA 
TCAAATGCTA AATATTATTC CAACGGCCTG CAGAAACCAG TATTCCTGGA AAAGGAGAAT 
GATAATAAGG GAATTTCTTT ATGTAAAACT ATCTCAGACG GTCAAAACAC TTCAGAAGGT 
GCCAGGGCTC CAGACTATTT TGAGGAACGT GTTCCTGAAA CAAAAGATAG ATGCAAAGCT 
ACACTAAGCA TCAGGGAAAA GGTTGTGGCT ACAGCAAATT GTTTTGCAAG AAAACAGCTT 
GAAACAGATT ATCCAGAGGA TGCCTCAGCA CAACATGAGG CACTTCAGTT CCATGCTAAG 
CAATATGTGT CATGCTCAAT GCTAAATTCT AATGTGTCCA ATGACATGCA AATAAAGCCT 
CAAGTGTTAA CTCAGGCATG CGATTTTAGG AGCAGCGCAG AAAATCTTGT TGAAAAAACA 
AAAAATCAAT TAAGGCGGAA TGAAGATCAA TCATTTTCAT CAACTGGCAT AGGGAAAAAG 
CCAGCTAGTG GTGAAAAATT AGTAGGTGAC TGTAATGAGG GCTCATTCCT TCATGTGAAG 
CAAGAAATAA GTGCTGCTCA GGCATTGGTA GGTAATGGTT ACGAAACCAG AGACACTCCT 
GATGTCACTA GTGATCATAC AAGGAGTGTT ATTTCCAACC ATGGAAAGTC TCTGTGGGAG 
GAGGAGACAG CACTGACTGA ATTACCAGTC CCAGAAATTC TGAGTAAAAC TTTCAAGGGC 
TTTAAAACGG CATCTAATAA AAAAATACAT ATTTCAGATA AAAATATTGT AAAAGGTTGT 
GACTTGTTCA AAGAAATTGA GTTTGGCATT GGTGACGCTA AGCCCTGCAA TGAAGAAAAC 
AAAGAGCCCT TAAAGAAAAC TCCCTTTGAT GTTCCTGGGT ATGAAACAAA TTTAAAAGGA 
TTCAAAACTG CATCCAATAA AGAAATAAAT GTATCAGAAA ACAAATTTGC TAAAGGTAGA 
CTGCTGTTTA AAGACATCGA AGAGGAAAGC GGTCAAACTA CAGCTATAAT GGCAAAAGAT 
AATGAGCTGT TCATTAAGCC TGCATCCAGC AATAAGCCTG ACATATTTAA GAGTACATGT 
ATAAAAGGAA TGAATCCATC TGAGAACCTA GACAGTATGC CCCCCAAAAA AGGAGACGTG 
CAGCAGAGTG CAGGTTTAAA ACATTCAGAT CAAATTGACG TTTTTGGTTT CGATGATCCT 
CCTGCAAAAG GAAATGTTCA AACATTAGGC TATTTTAATA TGTCTGCTTC TGAAACAGAC 
TTCAAAGGAT TCAAAACCGC TTCCAATAAA GACATAATTA TTTCAGAAAG CACACTTGCT 
AAAGGTAAAC TTATTTTTGA AGATATTGAA GATACAAGAT ACAGTGAAAC AGGAAGGACC 
ATAGATTGTA AAGCAAAAGG TGGTGCGTTG TCCACCAGTA ACCTTGTAAT GCATAACACC 
ATGTGTAAAA ATGAACCAAG TACATCTGAG AACACAAATG ATAAAGCACC AAAAAGAGCA 
AAAGTACAAA CACCTATGGC AGAAAATAGT CATTTAAAAG AACCTTCTTC AGACAAAAAT 
GCAATGGATG CTGCAAATCA TCAAATGACA CTGAAAGCCT CCGCATACAG CCCAAAGGCT 
GCCTCTTCTG CACTGCCCAG GTACAAAAAG AAACCAATCT CTACCACTAA AACCTCTTTC 
CAGTTGAATG AACATTTGAC TGAAAGCCAG CAAGCTGAAA TCTCTGAACT TTCCTCTATT 
CTAGAAAATG CAGACAGTCA GTTTGATTTT ACTCAGTTTA GGAAAGTGCC TTCTGTGACT 
GAGAAACAAA ATTCAACTGA AGGTGGGAGC GAGTCCCAGA ACCTTAACAA CTCAGATGTC 
TGGAAGGACG TAGATTTTAA TGATAGTTTT GCAGCAGGAA GGGATCATTC TGAGGGAATG 
GAGGCAATTC CCTCCAGTCC TGGAATAAAA GAATTGCCTT CTGCAGGAGA ATGTTGCAGT 
AAAGAAACAC ATGATTCTAT GCCAGATTTT GTGACCTTGG CACCTAAACA AGGTTTTCTG 
GTTAAACAAA ATGAAAGACT CTTTGCAGGG TTTAATCTTG CTAGTGGCAA GCAAGTTAAT 
ATAGACAATG ATGTACTAAA AAAAGCTGCT GAACTGTTCA ATGATATAGA TAATGATAAA 
GAATTGCTCA GCCACGCCAA AGAAGAAAGC AGAAAAAGCA ATATAAAACA CTCTTCTAAA 
TTAATAAATA ATGAAAATTG TGGAAAGACA GAACACACAT CTGAGCATTT GGTATGTCAA 
AGTAATGTTT CATTACCATT CATAAAAACA TTGGGCAAGA ATGCTACAAT ACTAGTGAGT 
GGTAATGAAA AAAGCCAGAA TCAAGAATTA CAGCTGGATT GTTTAAAATG TGAAAGTACT 
GATGTAAAGC ATACACCACA AAAGGAAACA ATCAATGACA ACTCAAAGTG TAATGAATCC 
TCTCTTTCTG AGCTTTCCAT GAAAGGTTTT CAGACTGCCA GTGGGAGAAA CATTATGATG 
TCCGAGTCTT CAATCCAGAA AGCCAGAAAC ATATTTGCAG AGGAACATGA AGACAGTTTT 
ACATTGCGCT GTAATATTCA AAATACAATA CAAATACCAC AGCCTGTAAA TGAACCGACC 
CAATTCCCAT ATGTTAATTT GGGGCCAAAA CCCACAACGA CATCAGGATG GCAAGAAAAA 
AATATTCTGA GAAGAAGTAC AGAAAAAGGT TTCATGCCGG GTTTTTGCAC TGCCGGTGGT 
AAAAAGGTTT CTGTTTCCGA CGACTCTCTA GCTAAAGCAC ATAAACTTTT CCAGGAGGAA 
TGTACCTTTA GCAAGGAAGG GAAATTGGAT GAGGTGAAAC AAAATAAATT AATGAACTCT 
GAGCCATTGT CTCTTCTCAC TTGTGAGTCA GTCCTGAAAC AGAGTGACGG ATTCATTGAA 
GATATTTCAA CTTCTAGAAA TGCTTTGGAA ATCAGACCAG AGCTTTATCC GGAGGGCATG 
TGTAGTAATA GGGCATCTAA TGGAAGTGGA AACAATAGTG AATTTACAGC TGGGGAAGGC 
ATATCAATAA ATATCAACCA ATCATCGCTG CTTACAACAG GCAACGTTTT AAAAAATTTG 
CCGTCTGAAA GTAGTGGCCA TGATGTGTAT TCAGTAACTG AGCATCTAAG CACTGTGGTT 
AAAGTCAAAA GATACAATGA TTCAGGTCAT TTTGTAAATC AAAATTTAGC TGAATGTAAT 
GATAACCATG TCCTTAGCAC TCAAAAAAAT ACTGCAAATA TAAGCAACAG AAACGAGGAC 
TGTACATCCC TGGCACCTCT CTCTTTCAGC ACAGCCAGTG GGAAATCCGT CACAGTGTCT 
CATGATTCTC TTCAAAAAGC AAGACTGATG CTTTCTGAAG CAGCTAATGA TGTTACAGTA 
GATACTAGTA AGCAAGAGGC GGCTTATATT ACGCCAGCCA TTCGAAAAAC AGAAGCAGAA 
AAAGAGCAGA ACACTGTTGA TGATTCTGAT AGAGTTAATG CAAATACATT TTCATTTTCT 
ACTGCAAGCG GTAAAAAGGT CAACATAAGC GGAAATTCTT TAAAGCAGGT CAGAGCTGTC 
TGCCTATCCT CTGACCCAAA AGAAACATCA GCGGCTCTGT TTAATGTTGA AAAAAGTGTT 
TTTAATGAAG ATGTTAAGGA TGTATCTTTG CTTCAGCCCA ATGTTACCAT GCCGAAGGCT 
GTGTCGTTTA GTACTGCAAG TGGCAAGACG GTGCAGTTGT CAGACGAGTC TTTGAAGAAA 
GCTAGGGTGA TTTTTTCTGA AATAGATACT TGTCCGTTGA TGCAACAGCA GACTAATGAA 
AGCACTGTTG AAGAGATAGT AATAGGAGGA GGAATGACAA AATCAAAACA GATGCCATTA 
ACAACAGAAA AAGTCGAAAC AACTAGAAAA AATAATGGTA CTTTTGGCTT TAATACCGCA 
AGCGGAAAGC AGGTCTCTGT GTCTGAAAGT GCGCTGCAGA AAGTCAAAGA TATCTTTCAA 
GAATTTGATG ATCCCGACAA TTATGAACAA AATAAATCAC TCGTAAGACT GCCTGTTTCA 
TCAAAAATAA AAGAATCAAC TCCAGGAACA AAAAGACTGG TGCAAACTGC AGGGTCCTCT 
TATAAAAATG ATAATTTGCA ATGTAAAGCA GGGAATCTTC GTACTTTTCA GGATAAGCAA 
GCTGGCAAGA AGTCACTTAC ATATTCAGAA GCAGCCATAA GCCCCATTAT AACAACTGTC 
CCTGGAAATC AATTAACGCC CATCCAGTTG GTAAAAGTCT CAACCAACAC ATCAGCATTG 
GCTAATAAAA CCAAACCCGA TTTATATGTC GCAACAGCCC AGAACACACC ACAAAATGAT 
TTTGAAATAG AAGCAGCAGA AAGTGCTAGA GCTTTCTTGG AAGATGATGA CCTGACAGAT 
ATGGGATCAG CTGCCCATGA AACATACTTG TATCTCAACC AGACCTCAAA TACAAGAACT 
GGGAAGAGAT CAAGAACAGA AGGGCCGACA CCTGGAGAGC CACCAATCAA AAGACGGCTT 
TTACCAGAAT TTGATTGCAC AACAGACACT CGGCAACAAA CGGGGATTTT AAAGCCTTTG 
ATCAGCACTC CAGACATGAT GCGAGACAGG CGAAGGTTTT TGTACACCCT TCCCTTGAAG 
CCATCAACTT GTAATCCTGA AAGTGCTTTA CGTCAAGTCA CGTGCATTCC TACACAAGCC 
CATCTCCATT CTAAAGTCAA AATCTTCCAC CAGTCTCTAC CCATCAAGTC ACCAGATGTT 
GCATCTGATT CAACTTCTAA ATCGTATAGC CCCACTGCTG CAAAAGAGAC CATAAACTGC 
TCATCTGCAT CTAAAATACC TGCAAAGAAA TTTGTGCCTC CTTTTAAAAA GACTGTGGCG 
ACCTTGGCAG ACAACCAGTC AAACAGTGTA CAAAATGGCA GTTCGGATGG GCTTATCGAG 
AGCATTGTTT ATCCAAAAGA AGACAAAGTT GAAACCATAT GTTCTTCAAA AGATCAATTT 
GATGACTCAG ATATTTTACA AATGACTTCA AATCTTCGAT GTAGCAAAGA CCTGCAGGAA 
ATGAGAATTC GGAAAAAGCT AAGGCAAAAG ATTAAACCGC ATCCAGGCTC ACTGTATCGT 
CTGAAAATGT CACATGTGAA GAGAATTTCA CTTCAATCTG CAGTGGCAGA AAGGTGTCCC 
ACTCTGTATT CAAGGGAACA GTTATACAGA TATGGCATTG TGAAGAACCA TATTGGAGTC 
AGCAGCGAGA ACGCATTGTC CTTCCAGTTC CACTGTTCAA ATTATTTCAC AAAAGAACTT 
CTTCTTTCTG GAAATGGTGT GCAGTTGGCA GATGGAGGTT GGCTTATTCC CACTGAACAA 
GGCAACGCAG GCAAAGAAGA GATATACAGA GCGTTTTGTG ACACTCCTGG TGTGGATCCA 
AAATTAATTT CAGCTGAATG GGTTCATAAC CACTACCGCT GGATTGTATG GAAACTTGCT 
GCAATGGAAG TCAGGTTCCC AAAGACATTT GCTTGCAGAT GCCTTACACC AGAACGTGTG 
CTTTTACAGT TAAAGTACAG ATATGATGTG GAAATTGATA AGAGTCAGAG ATCCGCCATC 
AAAAAGATCA TGGAGAGAGA TGACTCCCCA GCTAAAACGC TGGTACTGTG CATTGCCAAG 
ATCATTTCCC AGGGCACTCG CCTGCCCAAT GCATGTAGTA ATAAAACCGA ACCCGCTGAC 
TCTAAGGAAA GTTCTGCAGT AATAGAGGTG ACAGATAGTT GGTATGGTAT CAAGGTTCTG 
CTTGATCCGT GTCTCACTGC TCTCTTACAC AAAGGAAGGC TTTTTATTGG TCAGAAGCTA 
ATTGTTCATG GAGCGGAACT TATTGGCTCA GATGATGCCT GCTCTCCCCT TGAAGCGCCA 
GAATCTCTTA TGTTGAAGAT TGCAGCTAAC AGCACTCGGC CTGTTCGATG GCATACCAAA 
CTTGGATATT TCAAGGATCC CAGACCATTC TGTCTCCATT TATCATCACT GTTAAGTGAA 
GGAGGTGTGG TGGGGTGTGT CGATGTAGTA ATACAAAGAA TCTATCCCAT GCAGTGGATG 
GAAAAAATGG CAAATGGACT GTATGTGTTT CGCAATGATC GAGCAGAGGA AAGAGAAGCA 
GAAAAGCATT CAGCAAATCA ACAAAAAAAG CTTGAAATGC TCTTTTCAAA AATACAAGCT 
GAATTTGAAC AGCGGGAAGT AACATGCAAC AGAAGAAAGG GACTCAGGCG GCGGTCATTA 
AATGCACAAC AAATGCAGAC CCTTCAGGAT GGGGCTGAAA TCTATGAAGC CATTCAGAAC 
GAATCTGACC CTGGGTATCT GGAGTCGTAC TTAAGTGCTG AGCAGCTGAA AGCCCTCAAT 
CACCACAGGC AACTTTTAAA CGATAAGAAG CAAGCACTGA TCCAAGCTGA ATTCAGAAAA 
GCCATTGAAT GCAGTGAGCA GGATGCAAAT GGCTGTACAA GGAGGGATGT CACTCCTGTA 
TGGAAATTAC GCATTGCAGA TTACAGAAAT TATGAAACAG ATGCAGCATA TATACTGAAC 
ATTTGGCGTC CGTTACCAGA TGTGCTTTCT CTTCTGAAAG AAGGTTGTCG CTATAAAATG 
TATCATTTGG CAGCATCCAC ATCAAAAGGA AAGTCATTAG CAGCTGATTT GCAGTTAACA 
GCTACAAAGA AAACTCGCTT CCAGCAACTT CAGCCATCAG AGAGCATTTT GGAGCAGATT 
TACAGCCCGA GAGAAGTGAC GGACTTCAGC AGGTTCCAGG AGCCTTTGTT TAGCGCCCCA 
TATGCAGAGG TTGATCTGGT GGGATTAATC ATCTCCATAT ACAAAAAAAC AGGTGCAGCT 
CCAGTGGTTT ATATATCAGA TGAATCTCAC AATATTGTGG CGCTAAAATT CTGGACAGAT 
CTAGGGCAGC TTGGGCTTGA GGAGATAACC AAGCCACGCA CATATATCTC TGCCAGTAAT 
CTTCGCTGGA GATCAGACTG TATCGAGGGG ATTCCTACAC TGTACGTTGG AGACTTAGCC 
AACATTTCTT CCAATCCCAA AGAAAGCCAT CTGCAGAGGG CAATTCAGAA ACTGAAACTT 
TCTGTACAGA ATGTCCAAGA TTTTTGGAAT TCATCACAAA CCGCACTGAT GAAAACCTTG 
CAGATAAATA GCACAGACAC AACGGAGTGC AGTAAGAACC CAACGACACC AACATGGAAG 
TCAGATGTTT CTGCTAGAAG TGGTTACCTA ACCCCCTTGC ACCATAGTGG TAAAAGGTTA 
TTAAATTCTG TCCATACATC AGATCCTCAG ACAGAAAATC CTGGATGTAG TAAAGAAATA 
CAACTAAAAA CCTGCAAAAA GAGGAAAGCT CTGGACTTTC TCAACCGCAT TCCTTCACCT 
CCACCTGTAA CACCAGTACG GCCCTTTGTA TCCCCATCTT TGCAGAAGGC ATTCCGACCC 
CCACGGAGCT GTAGCGTCCA GAAGTTGGGC CCGGAAACCA AGGGTAATAC TGAGAATGTC 
CAGGGAACAA CCCCAGAATG CACTAAAGAC CTAGCAAAAT TAGAGGGTGA ATTTGTGGCT 
GATGAAGAAC TCGCCATGAT CAATACCCAA GCTTTGCTTT TAGGTCTGGA GGAAGAAAAA 
AAGAAAACCG AGCAGAAAAC CAGCAGAACA GCAGGTAAAA TGACTGCCCA TGAGTCTCCA 
ATAGAAAATG CTTCCCCCGT TCCTGCCCAG GAACAACAAA CTGAGGAAGC ATTGAACATT 
CCAGTCGGTA ACTCAGAGAA ATCTTACCTG TGCCTAAGAA AAAGGAAACG GAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031753095.1
CDS	237..9953
Translation	MAAPQLGKSVFYDLFSTHCSHSDLGPISLNWFEELTAEALPYKSRTCEDHECSLDDLHENNIKTPKLKSSIYSQLDSTPVIFKERLLSPLFASSLTELDKRQNATDNGNNVRLEKSNCTQMQHQASEVFSPSSRCLNESPAVIKEIFKTPLRNKYLHKTPQCDWKPDICSSLFCTPKLMKNQTGCIKESLGAEVDPEMSWSSSLATPPSPTVIIAHANDQPSGNKHAAIVQSLFPNCENMEANNLPPPETGTQNEDRQICGMGSVIQKIPERSSSIDSIITGLEKPGSCSASKTVPEASAISSRAAWKKTVANTVKDEEVSRTVENALEGMEDVLSIFFASEKTPGLRKLKNSTQARRKVESIKSQKCQVSLFKAEEGESILVPLDEHHKCDHEMLMKPEQDYKMNAVNQKIAKETTPYEWSQLNICELDITQSQDSTLHAANLCCEDITVNKSDTCNITEAEKQEEVNEPSTDNVLSNKVGKESTLNVNCINADLNNMASSSNNSVHSLNLSQCEKMDSSEISNSEVGCITKLTTHPAKITTGELSSVDDCAKKPQERVTISTSFSTLKKQSKFMYSVNTVLTGHIASNTSIATRRSLNSHLSSDEPKPHIKENQNEPESNAKYYSNGLQKPVFLEKENDNKGISLCKTISDGQNTSEGARAPDYFEERVPETKDRCKATLSIREKVVATANCFARKQLETDYPEDASAQHEALQFHAKQYVSCSMLNSNVSNDMQIKPQVLTQACDFRSSAENLVEKTKNQLRRNEDQSFSSTGIGKKPASGEKLVGDCNEGSFLHVKQEISAAQALVGNGYETRDTPDVTSDHTRSVISNHGKSLWEEETALTELPVPEILSKTFKGFKTASNKKIHISDKNIVKGCDLFKEIEFGIGDAKPCNEENKEPLKKTPFDVPGYETNLKGFKTASNKEINVSENKFAKGRLLFKDIEEESGQTTAIMAKDNELFIKPASSNKPDIFKSTCIKGMNPSENLDSMPPKKGDVQQSAGLKHSDQIDVFGFDDPPAKGNVQTLGYFNMSASETDFKGFKTASNKDIIISESTLAKGKLIFEDIEDTRYSETGRTIDCKAKGGALSTSNLVMHNTMCKNEPSTSENTNDKAPKRAKVQTPMAENSHLKEPSSDKNAMDAANHQMTLKASAYSPKAASSALPRYKKKPISTTKTSFQLNEHLTESQQAEISELSSILENADSQFDFTQFRKVPSVTEKQNSTEGGSESQNLNNSDVWKDVDFNDSFAAGRDHSEGMEAIPSSPGIKELPSAGECCSKETHDSMPDFVTLAPKQGFLVKQNERLFAGFNLASGKQVNIDNDVLKKAAELFNDIDNDKELLSHAKEESRKSNIKHSSKLINNENCGKTEHTSEHLVCQSNVSLPFIKTLGKNATILVSGNEKSQNQELQLDCLKCESTDVKHTPQKETINDNSKCNESSLSELSMKGFQTASGRNIMMSESSIQKARNIFAEEHEDSFTLRCNIQNTIQIPQPVNEPTQFPYVNLGPKPTTTSGWQEKNILRRSTEKGFMPGFCTAGGKKVSVSDDSLAKAHKLFQEECTFSKEGKLDEVKQNKLMNSEPLSLLTCESVLKQSDGFIEDISTSRNALEIRPELYPEGMCSNRASNGSGNNSEFTAGEGISININQSSLLTTGNVLKNLPSESSGHDVYSVTEHLSTVVKVKRYNDSGHFVNQNLAECNDNHVLSTQKNTANISNRNEDCTSLAPLSFSTASGKSVTVSHDSLQKARLMLSEAANDVTVDTSKQEAAYITPAIRKTEAEKEQNTVDDSDRVNANTFSFSTASGKKVNISGNSLKQVRAVCLSSDPKETSAALFNVEKSVFNEDVKDVSLLQPNVTMPKAVSFSTASGKTVQLSDESLKKARVIFSEIDTCPLMQQQTNESTVEEIVIGGGMTKSKQMPLTTEKVETTRKNNGTFGFNTASGKQVSVSESALQKVKDIFQEFDDPDNYEQNKSLVRLPVSSKIKESTPGTKRLVQTAGSSYKNDNLQCKAGNLRTFQDKQAGKKSLTYSEAAISPIITTVPGNQLTPIQLVKVSTNTSALANKTKPDLYVATAQNTPQNDFEIEAAESARAFLEDDDLTDMGSAAHETYLYLNQTSNTRTGKRSRTEGPTPGEPPIKRRLLPEFDCTTDTRQQTGILKPLISTPDMMRDRRRFLYTLPLKPSTCNPESALRQVTCIPTQAHLHSKVKIFHQSLPIKSPDVASDSTSKSYSPTAAKETINCSSASKIPAKKFVPPFKKTVATLADNQSNSVQNGSSDGLIESIVYPKEDKVETICSSKDQFDDSDILQMTSNLRCSKDLQEMRIRKKLRQKIKPHPGSLYRLKMSHVKRISLQSAVAERCPTLYSREQLYRYGIVKNHIGVSSENALSFQFHCSNYFTKELLLSGNGVQLADGGWLIPTEQGNAGKEEIYRAFCDTPGVDPKLISAEWVHNHYRWIVWKLAAMEVRFPKTFACRCLTPERVLLQLKYRYDVEIDKSQRSAIKKIMERDDSPAKTLVLCIAKIISQGTRLPNACSNKTEPADSKESSAVIEVTDSWYGIKVLLDPCLTALLHKGRLFIGQKLIVHGAELIGSDDACSPLEAPESLMLKIAANSTRPVRWHTKLGYFKDPRPFCLHLSSLLSEGGVVGCVDVVIQRIYPMQWMEKMANGLYVFRNDRAEEREAEKHSANQQKKLEMLFSKIQAEFEQREVTCNRRKGLRRRSLNAQQMQTLQDGAEIYEAIQNESDPGYLESYLSAEQLKALNHHRQLLNDKKQALIQAEFRKAIECSEQDANGCTRRDVTPVWKLRIADYRNYETDAAYILNIWRPLPDVLSLLKEGCRYKMYHLAASTSKGKSLAADLQLTATKKTRFQQLQPSESILEQIYSPREVTDFSRFQEPLFSAPYAEVDLVGLIISIYKKTGAAPVVYISDESHNIVALKFWTDLGQLGLEEITKPRTYISASNLRWRSDCIEGIPTLYVGDLANISSNPKESHLQRAIQKLKLSVQNVQDFWNSSQTALMKTLQINSTDTTECSKNPTTPTWKSDVSARSGYLTPLHHSGKRLLNSVHTSDPQTENPGCSKEIQLKTCKKRKALDFLNRIPSPPPVTPVRPFVSPSLQKAFRPPRSCSVQKLGPETKGNTENVQGTTPECTKDLAKLEGEFVADEELAMINTQALLLGLEEEKKKTEQKTSRTAGKMTAHESPIENASPVPAQEQQTEEALNIPVGNSEKSYLCLRKRKRK

Target ORF information:

RefSeq Version	XM_031897235.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis BRCA2, DNA repair associated (brca2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031897235.1

ORF Insert Sequence:

ATGGCTGCAC CGCAACTTGG AAAATCTGTC TTCTATGATC TGTTTAGCAC GCATTGCTCT 
CACTCAGATT TAGGGCCTAT CAGTCTAAAC TGGTTTGAAG AGCTCACTGC TGAAGCCTTG 
CCTTACAAGT CAAGAACATG TGAAGATCAT GAATGCAGTT TGGATGATCT CCATGAAAAC 
AACATCAAAA CTCCTAAACT GAAGTCGTCA ATTTATAGCC AATTAGATTC CACTCCAGTC 
ATCTTTAAGG AGCGTTTACT ATCACCTCTT TTTGCTTCTT CTTTGACAGA GCTGGATAAA 
AGGCAGAATG CAACAGATAA TGGAAACAAT GTTAGACTTG AGAAAAGTAA CTGTACCCAA 
ATGCAGCATC AAGCAAGTGA AGTTTTCAGT CCCTCAAGTC GATGCCTCAA CGAAAGTCCT 
GCTGTTATCA AAGAAATATT CAAGACACCA TTACGAAATA AGTACCTACA TAAAACACCA 
CAGTGTGATT GGAAACCTGA CATTTGCAGC AGTCTGTTTT GTACTCCAAA GCTTATGAAG 
AACCAAACTG GATGTATTAA AGAAAGTCTA GGTGCAGAGG TGGATCCAGA GATGTCATGG 
TCTAGTTCAC TAGCTACACC TCCTTCCCCA ACTGTGATAA TTGCACATGC TAATGATCAG 
CCCAGTGGAA ATAAACATGC TGCAATTGTA CAGAGCCTTT TCCCAAATTG TGAGAATATG 
GAAGCGAATA ATTTGCCACC ACCTGAGACG GGCACACAAA ATGAAGACCG TCAAATATGT 
GGTATGGGAT CTGTTATCCA GAAAATTCCG GAAAGGTCAT CTTCCATAGA CTCCATTATA 
ACAGGACTAG AAAAGCCAGG TTCATGCAGT GCGAGCAAAA CTGTACCAGA AGCGTCTGCC 
ATTTCCTCAA GGGCAGCCTG GAAGAAAACT GTTGCAAATA CAGTGAAGGA CGAGGAGGTT 
TCCAGAACTG TGGAAAATGC TCTGGAAGGA ATGGAAGATG TCCTCTCAAT CTTTTTTGCA 
AGTGAAAAGA CTCCTGGTCT CAGAAAGCTG AAAAATTCTA CCCAAGCTAG GAGAAAAGTG 
GAATCTATTA AGTCTCAGAA GTGCCAAGTT TCATTATTTA AAGCAGAAGA AGGAGAAAGC 
ATTTTGGTCC CTTTAGATGA GCATCATAAA TGTGATCATG AGATGCTTAT GAAACCTGAA 
CAAGACTACA AAATGAATGC TGTTAACCAA AAAATAGCCA AAGAAACTAC TCCATATGAA 
TGGTCACAGC TAAATATATG TGAGTTGGAT ATAACTCAAT CACAGGATAG CACTTTGCAT 
GCAGCAAATC TATGTTGTGA AGATATCACT GTCAACAAAA GTGATACATG TAATATTACT 
GAAGCCGAAA AGCAGGAGGA AGTCAATGAG CCAAGCACCG ACAATGTACT GTCTAATAAA 
GTTGGCAAAG AAAGCACTTT AAATGTGAAT TGTATTAATG CTGATTTGAA TAACATGGCT 
TCATCCTCAA ATAACTCTGT TCACTCACTG AATCTTTCTC AATGTGAAAA AATGGATTCT 
TCTGAAATTT CTAACAGTGA AGTTGGTTGT ATAACAAAAC TAACAACCCA CCCAGCAAAA 
ATAACAACTG GTGAATTAAG TTCAGTAGAT GACTGTGCTA AGAAACCCCA GGAAAGAGTA 
ACTATTAGTA CTTCATTTTC AACTCTGAAA AAGCAATCGA AGTTTATGTA CAGTGTAAAC 
ACTGTGTTAA CTGGTCATAT TGCAAGCAAT ACCAGCATAG CCACTAGGAG ATCATTGAAC 
AGTCATTTAA GTTCTGATGA ACCAAAACCT CACATTAAGG AAAATCAAAA TGAACCAGAA 
TCAAATGCTA AATATTATTC CAACGGCCTG CAGAAACCAG TATTCCTGGA AAAGGAGAAT 
GATAATAAGG GAATTTCTTT ATGTAAAACT ATCTCAGACG GTCAAAACAC TTCAGAAGGT 
GCCAGGGCTC CAGACTATTT TGAGGAACGT GTTCCTGAAA CAAAAGATAG ATGCAAAGCT 
ACACTAAGCA TCAGGGAAAA GGTTGTGGCT ACAGCAAATT GTTTTGCAAG AAAACAGCTT 
GAAACAGATT ATCCAGAGGA TGCCTCAGCA CAACATGAGG CACTTCAGTT CCATGCTAAG 
CAATATGTGT CATGCTCAAT GCTAAATTCT AATGTGTCCA ATGACATGCA AATAAAGCCT 
CAAGTGTTAA CTCAGGCATG CGATTTTAGG AGCAGCGCAG AAAATCTTGT TGAAAAAACA 
AAAAATCAAT TAAGGCGGAA TGAAGATCAA TCATTTTCAT CAACTGGCAT AGGGAAAAAG 
CCAGCTAGTG GTGAAAAATT AGTAGGTGAC TGTAATGAGG GCTCATTCCT TCATGTGAAG 
CAAGAAATAA GTGCTGCTCA GGCATTGGTA GGTAATGGTT ACGAAACCAG AGACACTCCT 
GATGTCACTA GTGATCATAC AAGGAGTGTT ATTTCCAACC ATGGAAAGTC TCTGTGGGAG 
GAGGAGACAG CACTGACTGA ATTACCAGTC CCAGAAATTC TGAGTAAAAC TTTCAAGGGC 
TTTAAAACGG CATCTAATAA AAAAATACAT ATTTCAGATA AAAATATTGT AAAAGGTTGT 
GACTTGTTCA AAGAAATTGA GTTTGGCATT GGTGACGCTA AGCCCTGCAA TGAAGAAAAC 
AAAGAGCCCT TAAAGAAAAC TCCCTTTGAT GTTCCTGGGT ATGAAACAAA TTTAAAAGGA 
TTCAAAACTG CATCCAATAA AGAAATAAAT GTATCAGAAA ACAAATTTGC TAAAGGTAGA 
CTGCTGTTTA AAGACATCGA AGAGGAAAGC GGTCAAACTA CAGCTATAAT GGCAAAAGAT 
AATGAGCTGT TCATTAAGCC TGCATCCAGC AATAAGCCTG ACATATTTAA GAGTACATGT 
ATAAAAGGAA TGAATCCATC TGAGAACCTA GACAGTATGC CCCCCAAAAA AGGAGACGTG 
CAGCAGAGTG CAGGTTTAAA ACATTCAGAT CAAATTGACG TTTTTGGTTT CGATGATCCT 
CCTGCAAAAG GAAATGTTCA AACATTAGGC TATTTTAATA TGTCTGCTTC TGAAACAGAC 
TTCAAAGGAT TCAAAACCGC TTCCAATAAA GACATAATTA TTTCAGAAAG CACACTTGCT 
AAAGGTAAAC TTATTTTTGA AGATATTGAA GATACAAGAT ACAGTGAAAC AGGAAGGACC 
ATAGATTGTA AAGCAAAAGG TGGTGCGTTG TCCACCAGTA ACCTTGTAAT GCATAACACC 
ATGTGTAAAA ATGAACCAAG TACATCTGAG AACACAAATG ATAAAGCACC AAAAAGAGCA 
AAAGTACAAA CACCTATGGC AGAAAATAGT CATTTAAAAG AACCTTCTTC AGACAAAAAT 
GCAATGGATG CTGCAAATCA TCAAATGACA CTGAAAGCCT CCGCATACAG CCCAAAGGCT 
GCCTCTTCTG CACTGCCCAG GTACAAAAAG AAACCAATCT CTACCACTAA AACCTCTTTC 
CAGTTGAATG AACATTTGAC TGAAAGCCAG CAAGCTGAAA TCTCTGAACT TTCCTCTATT 
CTAGAAAATG CAGACAGTCA GTTTGATTTT ACTCAGTTTA GGAAAGTGCC TTCTGTGACT 
GAGAAACAAA ATTCAACTGA AGGTGGGAGC GAGTCCCAGA ACCTTAACAA CTCAGATGTC 
TGGAAGGACG TAGATTTTAA TGATAGTTTT GCAGCAGGAA GGGATCATTC TGAGGGAATG 
GAGGCAATTC CCTCCAGTCC TGGAATAAAA GAATTGCCTT CTGCAGGAGA ATGTTGCAGT 
AAAGAAACAC ATGATTCTAT GCCAGATTTT GTGACCTTGG CACCTAAACA AGGTTTTCTG 
GTTAAACAAA ATGAAAGACT CTTTGCAGGG TTTAATCTTG CTAGTGGCAA GCAAGTTAAT 
ATAGACAATG ATGTACTAAA AAAAGCTGCT GAACTGTTCA ATGATATAGA TAATGATAAA 
GAATTGCTCA GCCACGCCAA AGAAGAAAGC AGAAAAAGCA ATATAAAACA CTCTTCTAAA 
TTAATAAATA ATGAAAATTG TGGAAAGACA GAACACACAT CTGAGCATTT GGTATGTCAA 
AGTAATGTTT CATTACCATT CATAAAAACA TTGGGCAAGA ATGCTACAAT ACTAGTGAGT 
GGTAATGAAA AAAGCCAGAA TCAAGAATTA CAGCTGGATT GTTTAAAATG TGAAAGTACT 
GATGTAAAGC ATACACCACA AAAGGAAACA ATCAATGACA ACTCAAAGTG TAATGAATCC 
TCTCTTTCTG AGCTTTCCAT GAAAGGTTTT CAGACTGCCA GTGGGAGAAA CATTATGATG 
TCCGAGTCTT CAATCCAGAA AGCCAGAAAC ATATTTGCAG AGGAACATGA AGACAGTTTT 
ACATTGCGCT GTAATATTCA AAATACAATA CAAATACCAC AGCCTGTAAA TGAACCGACC 
CAATTCCCAT ATGTTAATTT GGGGCCAAAA CCCACAACGA CATCAGGATG GCAAGAAAAA 
AATATTCTGA GAAGAAGTAC AGAAAAAGGT TTCATGCCGG GTTTTTGCAC TGCCGGTGGT 
AAAAAGGTTT CTGTTTCCGA CGACTCTCTA GCTAAAGCAC ATAAACTTTT CCAGGAGGAA 
TGTACCTTTA GCAAGGAAGG GAAATTGGAT GAGGTGAAAC AAAATAAATT AATGAACTCT 
GAGCCATTGT CTCTTCTCAC TTGTGAGTCA GTCCTGAAAC AGAGTGACGG ATTCATTGAA 
GATATTTCAA CTTCTAGAAA TGCTTTGGAA ATCAGACCAG AGCTTTATCC GGAGGGCATG 
TGTAGTAATA GGGCATCTAA TGGAAGTGGA AACAATAGTG AATTTACAGC TGGGGAAGGC 
ATATCAATAA ATATCAACCA ATCATCGCTG CTTACAACAG GCAACGTTTT AAAAAATTTG 
CCGTCTGAAA GTAGTGGCCA TGATGTGTAT TCAGTAACTG AGCATCTAAG CACTGTGGTT 
AAAGTCAAAA GATACAATGA TTCAGGTCAT TTTGTAAATC AAAATTTAGC TGAATGTAAT 
GATAACCATG TCCTTAGCAC TCAAAAAAAT ACTGCAAATA TAAGCAACAG AAACGAGGAC 
TGTACATCCC TGGCACCTCT CTCTTTCAGC ACAGCCAGTG GGAAATCCGT CACAGTGTCT 
CATGATTCTC TTCAAAAAGC AAGACTGATG CTTTCTGAAG CAGCTAATGA TGTTACAGTA 
GATACTAGTA AGCAAGAGGC GGCTTATATT ACGCCAGCCA TTCGAAAAAC AGAAGCAGAA 
AAAGAGCAGA ACACTGTTGA TGATTCTGAT AGAGTTAATG CAAATACATT TTCATTTTCT 
ACTGCAAGCG GTAAAAAGGT CAACATAAGC GGAAATTCTT TAAAGCAGGT CAGAGCTGTC 
TGCCTATCCT CTGACCCAAA AGAAACATCA GCGGCTCTGT TTAATGTTGA AAAAAGTGTT 
TTTAATGAAG ATGTTAAGGA TGTATCTTTG CTTCAGCCCA ATGTTACCAT GCCGAAGGCT 
GTGTCGTTTA GTACTGCAAG TGGCAAGACG GTGCAGTTGT CAGACGAGTC TTTGAAGAAA 
GCTAGGGTGA TTTTTTCTGA AATAGATACT TGTCCGTTGA TGCAACAGCA GACTAATGAA 
AGCACTGTTG AAGAGATAGT AATAGGAGGA GGAATGACAA AATCAAAACA GATGCCATTA 
ACAACAGAAA AAGTCGAAAC AACTAGAAAA AATAATGGTA CTTTTGGCTT TAATACCGCA 
AGCGGAAAGC AGGTCTCTGT GTCTGAAAGT GCGCTGCAGA AAGTCAAAGA TATCTTTCAA 
GAATTTGATG ATCCCGACAA TTATGAACAA AATAAATCAC TCGTAAGACT GCCTGTTTCA 
TCAAAAATAA AAGAATCAAC TCCAGGAACA AAAAGACTGG TGCAAACTGC AGGGTCCTCT 
TATAAAAATG ATAATTTGCA ATGTAAAGCA GGGAATCTTC GTACTTTTCA GGATAAGCAA 
GCTGGCAAGA AGTCACTTAC ATATTCAGAA GCAGCCATAA GCCCCATTAT AACAACTGTC 
CCTGGAAATC AATTAACGCC CATCCAGTTG GTAAAAGTCT CAACCAACAC ATCAGCATTG 
GCTAATAAAA CCAAACCCGA TTTATATGTC GCAACAGCCC AGAACACACC ACAAAATGAT 
TTTGAAATAG AAGCAGCAGA AAGTGCTAGA GCTTTCTTGG AAGATGATGA CCTGACAGAT 
ATGGGATCAG CTGCCCATGA AACATACTTG TATCTCAACC AGACCTCAAA TACAAGAACT 
GGGAAGAGAT CAAGAACAGA AGGGCCGACA CCTGGAGAGC CACCAATCAA AAGACGGCTT 
TTACCAGAAT TTGATTGCAC AACAGACACT CGGCAACAAA CGGGGATTTT AAAGCCTTTG 
ATCAGCACTC CAGACATGAT GCGAGACAGG CGAAGGTTTT TGTACACCCT TCCCTTGAAG 
CCATCAACTT GTAATCCTGA AAGTGCTTTA CGTCAAGTCA CGTGCATTCC TACACAAGCC 
CATCTCCATT CTAAAGTCAA AATCTTCCAC CAGTCTCTAC CCATCAAGTC ACCAGATGTT 
GCATCTGATT CAACTTCTAA ATCGTATAGC CCCACTGCTG CAAAAGAGAC CATAAACTGC 
TCATCTGCAT CTAAAATACC TGCAAAGAAA TTTGTGCCTC CTTTTAAAAA GACTGTGGCG 
ACCTTGGCAG ACAACCAGTC AAACAGTGTA CAAAATGGCA GTTCGGATGG GCTTATCGAG 
AGCATTGTTT ATCCAAAAGA AGACAAAGTT GAAACCATAT GTTCTTCAAA AGATCAATTT 
GATGACTCAG ATATTTTACA AATGACTTCA AATCTTCGAT GTAGCAAAGA CCTGCAGGAA 
ATGAGAATTC GGAAAAAGCT AAGGCAAAAG ATTAAACCGC ATCCAGGCTC ACTGTATCGT 
CTGAAAATGT CACATGTGAA GAGAATTTCA CTTCAATCTG CAGTGGCAGA AAGGTGTCCC 
ACTCTGTATT CAAGGGAACA GTTATACAGA TATGGCATTG TGAAGAACCA TATTGGAGTC 
AGCAGCGAGA ACGCATTGTC CTTCCAGTTC CACTGTTCAA ATTATTTCAC AAAAGAACTT 
CTTCTTTCTG GAAATGGTGT GCAGTTGGCA GATGGAGGTT GGCTTATTCC CACTGAACAA 
GGCAACGCAG GCAAAGAAGA GATATACAGA GCGTTTTGTG ACACTCCTGG TGTGGATCCA 
AAATTAATTT CAGCTGAATG GGTTCATAAC CACTACCGCT GGATTGTATG GAAACTTGCT 
GCAATGGAAG TCAGGTTCCC AAAGACATTT GCTTGCAGAT GCCTTACACC AGAACGTGTG 
CTTTTACAGT TAAAGTACAG ATATGATGTG GAAATTGATA AGAGTCAGAG ATCCGCCATC 
AAAAAGATCA TGGAGAGAGA TGACTCCCCA GCTAAAACGC TGGTACTGTG CATTGCCAAG 
ATCATTTCCC AGGGCACTCG CCTGCCCAAT GCATGTAGTA ATAAAACCGA ACCCGCTGAC 
TCTAAGGAAA GTTCTGCAGT AATAGAGGTG ACAGATAGTT GGTATGGTAT CAAGGTTCTG 
CTTGATCCGT GTCTCACTGC TCTCTTACAC AAAGGAAGGC TTTTTATTGG TCAGAAGCTA 
ATTGTTCATG GAGCGGAACT TATTGGCTCA GATGATGCCT GCTCTCCCCT TGAAGCGCCA 
GAATCTCTTA TGTTGAAGAT TGCAGCTAAC AGCACTCGGC CTGTTCGATG GCATACCAAA 
CTTGGATATT TCAAGGATCC CAGACCATTC TGTCTCCATT TATCATCACT GTTAAGTGAA 
GGAGGTGTGG TGGGGTGTGT CGATGTAGTA ATACAAAGAA TCTATCCCAT GCAGTGGATG 
GAAAAAATGG CAAATGGACT GTATGTGTTT CGCAATGATC GAGCAGAGGA AAGAGAAGCA 
GAAAAGCATT CAGCAAATCA ACAAAAAAAG CTTGAAATGC TCTTTTCAAA AATACAAGCT 
GAATTTGAAC AGCGGGAAGT AACATGCAAC AGAAGAAAGG GACTCAGGCG GCGGTCATTA 
AATGCACAAC AAATGCAGAC CCTTCAGGAT GGGGCTGAAA TCTATGAAGC CATTCAGAAC 
GAATCTGACC CTGGGTATCT GGAGTCGTAC TTAAGTGCTG AGCAGCTGAA AGCCCTCAAT 
CACCACAGGC AACTTTTAAA CGATAAGAAG CAAGCACTGA TCCAAGCTGA ATTCAGAAAA 
GCCATTGAAT GCAGTGAGCA GGATGCAAAT GGCTGTACAA GGAGGGATGT CACTCCTGTA 
TGGAAATTAC GCATTGCAGA TTACAGAAAT TATGAAACAG ATGCAGCATA TATACTGAAC 
ATTTGGCGTC CGTTACCAGA TGTGCTTTCT CTTCTGAAAG AAGGTTGTCG CTATAAAATG 
TATCATTTGG CAGCATCCAC ATCAAAAGGA AAGTCATTAG CAGCTGATTT GCAGTTAACA 
GCTACAAAGA AAACTCGCTT CCAGCAACTT CAGCCATCAG AGAGCATTTT GGAGCAGATT 
TACAGCCCGA GAGAAGTGAC GGACTTCAGC AGGTTCCAGG AGCCTTTGTT TAGCGCCCCA 
TATGCAGAGG TTGATCTGGT GGGATTAATC ATCTCCATAT ACAAAAAAAC AGGTGCAGCT 
CCAGTGGTTT ATATATCAGA TGAATCTCAC AATATTGTGG CGCTAAAATT CTGGACAGAT 
CTAGGGCAGC TTGGGCTTGA GGAGATAACC AAGCCACGCA CATATATCTC TGCCAGTAAT 
CTTCGCTGGA GATCAGACTG TATCGAGGGG ATTCCTACAC TGTACGTTGG AGACTTAGCC 
AACATTTCTT CCAATCCCAA AGAAAGCCAT CTGCAGAGGG CAATTCAGAA ACTGAAACTT 
TCTGTACAGA ATGTCCAAGA TTTTTGGAAT TCATCACAAA CCGCACTGAT GAAAACCTTG 
CAGATAAATA GCACAGACAC AACGGAGTGC AGTAAGAACC CAACGACACC AACATGGAAG 
TCAGATGTTT CTGCTAGAAG TGGTTACCTA ACCCCCTTGC ACCATAGTGG TAAAAGGTTA 
TTAAATTCTG TCCATACATC AGATCCTCAG ACAGAAAATC CTGGATGTAG TAAAGAAATA 
CAACTAAAAA CCTGCAAAAA GAGGAAAGCT CTGGACTTTC TCAACCGCAT TCCTTCACCT 
CCACCTGTAA CACCAGTACG GCCCTTTGTA TCCCCATCTT TGCAGAAGGC ATTCCGACCC 
CCACGGAGCT GTAGCGTCCA GAAGTTGGGC CCGGAAACCA AGGGTAATAC TGAGAATGTC 
CAGGGAACAA CCCCAGAATG CACTAAAGAC CTAGCAAAAT TAGAGGGTGA ATTTGTGGCT 
GATGAAGAAC TCGCCATGAT CAATACCCAA GCTTTGCTTT TAGGTCTGGA GGAAGAAAAA 
AAGAAAACCG AGCAGAAAAC CAGCAGAACA GCAGGTAAAA TGACTGCCCA TGAGTCTCCA 
ATAGAAAATG CTTCCCCCGT TCCTGCCCAG GAACAACAAA CTGAGGAAGC ATTGAACATT 
CCAGTCGGTA ACTCAGAGAA ATCTTACCTG TGCCTAAGAA AAAGGAAACG GAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

brca2 ( XM_031897234.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031753094.1 Xenopus tropicalis BRCA2, DNA repair associated (brca2), transcript variant X1, mRNA.

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CloneID	OXa52805
Clone ID Related Accession (Same CDS sequence)	XM_031897235.1 , XM_031897234.1
Accession Version	XM_031897234.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	breast cancer type 2 susceptibility protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAC CGCAACTTGG AAAATCTGTC TTCTATGATC TGTTTAGCAC GCATTGCTCT 
CACTCAGATT TAGGGCCTAT CAGTCTAAAC TGGTTTGAAG AGCTCACTGC TGAAGCCTTG 
CCTTACAAGT CAAGAACATG TGAAGATCAT GAATGCAGTT TGGATGATCT CCATGAAAAC 
AACATCAAAA CTCCTAAACT GAAGTCGTCA ATTTATAGCC AATTAGATTC CACTCCAGTC 
ATCTTTAAGG AGCGTTTACT ATCACCTCTT TTTGCTTCTT CTTTGACAGA GCTGGATAAA 
AGGCAGAATG CAACAGATAA TGGAAACAAT GTTAGACTTG AGAAAAGTAA CTGTACCCAA 
ATGCAGCATC AAGCAAGTGA AGTTTTCAGT CCCTCAAGTC GATGCCTCAA CGAAAGTCCT 
GCTGTTATCA AAGAAATATT CAAGACACCA TTACGAAATA AGTACCTACA TAAAACACCA 
CAGTGTGATT GGAAACCTGA CATTTGCAGC AGTCTGTTTT GTACTCCAAA GCTTATGAAG 
AACCAAACTG GATGTATTAA AGAAAGTCTA GGTGCAGAGG TGGATCCAGA GATGTCATGG 
TCTAGTTCAC TAGCTACACC TCCTTCCCCA ACTGTGATAA TTGCACATGC TAATGATCAG 
CCCAGTGGAA ATAAACATGC TGCAATTGTA CAGAGCCTTT TCCCAAATTG TGAGAATATG 
GAAGCGAATA ATTTGCCACC ACCTGAGACG GGCACACAAA ATGAAGACCG TCAAATATGT 
GGTATGGGAT CTGTTATCCA GAAAATTCCG GAAAGGTCAT CTTCCATAGA CTCCATTATA 
ACAGGACTAG AAAAGCCAGG TTCATGCAGT GCGAGCAAAA CTGTACCAGA AGCGTCTGCC 
ATTTCCTCAA GGGCAGCCTG GAAGAAAACT GTTGCAAATA CAGTGAAGGA CGAGGAGGTT 
TCCAGAACTG TGGAAAATGC TCTGGAAGGA ATGGAAGATG TCCTCTCAAT CTTTTTTGCA 
AGTGAAAAGA CTCCTGGTCT CAGAAAGCTG AAAAATTCTA CCCAAGCTAG GAGAAAAGTG 
GAATCTATTA AGTCTCAGAA GTGCCAAGTT TCATTATTTA AAGCAGAAGA AGGAGAAAGC 
ATTTTGGTCC CTTTAGATGA GCATCATAAA TGTGATCATG AGATGCTTAT GAAACCTGAA 
CAAGACTACA AAATGAATGC TGTTAACCAA AAAATAGCCA AAGAAACTAC TCCATATGAA 
TGGTCACAGC TAAATATATG TGAGTTGGAT ATAACTCAAT CACAGGATAG CACTTTGCAT 
GCAGCAAATC TATGTTGTGA AGATATCACT GTCAACAAAA GTGATACATG TAATATTACT 
GAAGCCGAAA AGCAGGAGGA AGTCAATGAG CCAAGCACCG ACAATGTACT GTCTAATAAA 
GTTGGCAAAG AAAGCACTTT AAATGTGAAT TGTATTAATG CTGATTTGAA TAACATGGCT 
TCATCCTCAA ATAACTCTGT TCACTCACTG AATCTTTCTC AATGTGAAAA AATGGATTCT 
TCTGAAATTT CTAACAGTGA AGTTGGTTGT ATAACAAAAC TAACAACCCA CCCAGCAAAA 
ATAACAACTG GTGAATTAAG TTCAGTAGAT GACTGTGCTA AGAAACCCCA GGAAAGAGTA 
ACTATTAGTA CTTCATTTTC AACTCTGAAA AAGCAATCGA AGTTTATGTA CAGTGTAAAC 
ACTGTGTTAA CTGGTCATAT TGCAAGCAAT ACCAGCATAG CCACTAGGAG ATCATTGAAC 
AGTCATTTAA GTTCTGATGA ACCAAAACCT CACATTAAGG AAAATCAAAA TGAACCAGAA 
TCAAATGCTA AATATTATTC CAACGGCCTG CAGAAACCAG TATTCCTGGA AAAGGAGAAT 
GATAATAAGG GAATTTCTTT ATGTAAAACT ATCTCAGACG GTCAAAACAC TTCAGAAGGT 
GCCAGGGCTC CAGACTATTT TGAGGAACGT GTTCCTGAAA CAAAAGATAG ATGCAAAGCT 
ACACTAAGCA TCAGGGAAAA GGTTGTGGCT ACAGCAAATT GTTTTGCAAG AAAACAGCTT 
GAAACAGATT ATCCAGAGGA TGCCTCAGCA CAACATGAGG CACTTCAGTT CCATGCTAAG 
CAATATGTGT CATGCTCAAT GCTAAATTCT AATGTGTCCA ATGACATGCA AATAAAGCCT 
CAAGTGTTAA CTCAGGCATG CGATTTTAGG AGCAGCGCAG AAAATCTTGT TGAAAAAACA 
AAAAATCAAT TAAGGCGGAA TGAAGATCAA TCATTTTCAT CAACTGGCAT AGGGAAAAAG 
CCAGCTAGTG GTGAAAAATT AGTAGGTGAC TGTAATGAGG GCTCATTCCT TCATGTGAAG 
CAAGAAATAA GTGCTGCTCA GGCATTGGTA GGTAATGGTT ACGAAACCAG AGACACTCCT 
GATGTCACTA GTGATCATAC AAGGAGTGTT ATTTCCAACC ATGGAAAGTC TCTGTGGGAG 
GAGGAGACAG CACTGACTGA ATTACCAGTC CCAGAAATTC TGAGTAAAAC TTTCAAGGGC 
TTTAAAACGG CATCTAATAA AAAAATACAT ATTTCAGATA AAAATATTGT AAAAGGTTGT 
GACTTGTTCA AAGAAATTGA GTTTGGCATT GGTGACGCTA AGCCCTGCAA TGAAGAAAAC 
AAAGAGCCCT TAAAGAAAAC TCCCTTTGAT GTTCCTGGGT ATGAAACAAA TTTAAAAGGA 
TTCAAAACTG CATCCAATAA AGAAATAAAT GTATCAGAAA ACAAATTTGC TAAAGGTAGA 
CTGCTGTTTA AAGACATCGA AGAGGAAAGC GGTCAAACTA CAGCTATAAT GGCAAAAGAT 
AATGAGCTGT TCATTAAGCC TGCATCCAGC AATAAGCCTG ACATATTTAA GAGTACATGT 
ATAAAAGGAA TGAATCCATC TGAGAACCTA GACAGTATGC CCCCCAAAAA AGGAGACGTG 
CAGCAGAGTG CAGGTTTAAA ACATTCAGAT CAAATTGACG TTTTTGGTTT CGATGATCCT 
CCTGCAAAAG GAAATGTTCA AACATTAGGC TATTTTAATA TGTCTGCTTC TGAAACAGAC 
TTCAAAGGAT TCAAAACCGC TTCCAATAAA GACATAATTA TTTCAGAAAG CACACTTGCT 
AAAGGTAAAC TTATTTTTGA AGATATTGAA GATACAAGAT ACAGTGAAAC AGGAAGGACC 
ATAGATTGTA AAGCAAAAGG TGGTGCGTTG TCCACCAGTA ACCTTGTAAT GCATAACACC 
ATGTGTAAAA ATGAACCAAG TACATCTGAG AACACAAATG ATAAAGCACC AAAAAGAGCA 
AAAGTACAAA CACCTATGGC AGAAAATAGT CATTTAAAAG AACCTTCTTC AGACAAAAAT 
GCAATGGATG CTGCAAATCA TCAAATGACA CTGAAAGCCT CCGCATACAG CCCAAAGGCT 
GCCTCTTCTG CACTGCCCAG GTACAAAAAG AAACCAATCT CTACCACTAA AACCTCTTTC 
CAGTTGAATG AACATTTGAC TGAAAGCCAG CAAGCTGAAA TCTCTGAACT TTCCTCTATT 
CTAGAAAATG CAGACAGTCA GTTTGATTTT ACTCAGTTTA GGAAAGTGCC TTCTGTGACT 
GAGAAACAAA ATTCAACTGA AGGTGGGAGC GAGTCCCAGA ACCTTAACAA CTCAGATGTC 
TGGAAGGACG TAGATTTTAA TGATAGTTTT GCAGCAGGAA GGGATCATTC TGAGGGAATG 
GAGGCAATTC CCTCCAGTCC TGGAATAAAA GAATTGCCTT CTGCAGGAGA ATGTTGCAGT 
AAAGAAACAC ATGATTCTAT GCCAGATTTT GTGACCTTGG CACCTAAACA AGGTTTTCTG 
GTTAAACAAA ATGAAAGACT CTTTGCAGGG TTTAATCTTG CTAGTGGCAA GCAAGTTAAT 
ATAGACAATG ATGTACTAAA AAAAGCTGCT GAACTGTTCA ATGATATAGA TAATGATAAA 
GAATTGCTCA GCCACGCCAA AGAAGAAAGC AGAAAAAGCA ATATAAAACA CTCTTCTAAA 
TTAATAAATA ATGAAAATTG TGGAAAGACA GAACACACAT CTGAGCATTT GGTATGTCAA 
AGTAATGTTT CATTACCATT CATAAAAACA TTGGGCAAGA ATGCTACAAT ACTAGTGAGT 
GGTAATGAAA AAAGCCAGAA TCAAGAATTA CAGCTGGATT GTTTAAAATG TGAAAGTACT 
GATGTAAAGC ATACACCACA AAAGGAAACA ATCAATGACA ACTCAAAGTG TAATGAATCC 
TCTCTTTCTG AGCTTTCCAT GAAAGGTTTT CAGACTGCCA GTGGGAGAAA CATTATGATG 
TCCGAGTCTT CAATCCAGAA AGCCAGAAAC ATATTTGCAG AGGAACATGA AGACAGTTTT 
ACATTGCGCT GTAATATTCA AAATACAATA CAAATACCAC AGCCTGTAAA TGAACCGACC 
CAATTCCCAT ATGTTAATTT GGGGCCAAAA CCCACAACGA CATCAGGATG GCAAGAAAAA 
AATATTCTGA GAAGAAGTAC AGAAAAAGGT TTCATGCCGG GTTTTTGCAC TGCCGGTGGT 
AAAAAGGTTT CTGTTTCCGA CGACTCTCTA GCTAAAGCAC ATAAACTTTT CCAGGAGGAA 
TGTACCTTTA GCAAGGAAGG GAAATTGGAT GAGGTGAAAC AAAATAAATT AATGAACTCT 
GAGCCATTGT CTCTTCTCAC TTGTGAGTCA GTCCTGAAAC AGAGTGACGG ATTCATTGAA 
GATATTTCAA CTTCTAGAAA TGCTTTGGAA ATCAGACCAG AGCTTTATCC GGAGGGCATG 
TGTAGTAATA GGGCATCTAA TGGAAGTGGA AACAATAGTG AATTTACAGC TGGGGAAGGC 
ATATCAATAA ATATCAACCA ATCATCGCTG CTTACAACAG GCAACGTTTT AAAAAATTTG 
CCGTCTGAAA GTAGTGGCCA TGATGTGTAT TCAGTAACTG AGCATCTAAG CACTGTGGTT 
AAAGTCAAAA GATACAATGA TTCAGGTCAT TTTGTAAATC AAAATTTAGC TGAATGTAAT 
GATAACCATG TCCTTAGCAC TCAAAAAAAT ACTGCAAATA TAAGCAACAG AAACGAGGAC 
TGTACATCCC TGGCACCTCT CTCTTTCAGC ACAGCCAGTG GGAAATCCGT CACAGTGTCT 
CATGATTCTC TTCAAAAAGC AAGACTGATG CTTTCTGAAG CAGCTAATGA TGTTACAGTA 
GATACTAGTA AGCAAGAGGC GGCTTATATT ACGCCAGCCA TTCGAAAAAC AGAAGCAGAA 
AAAGAGCAGA ACACTGTTGA TGATTCTGAT AGAGTTAATG CAAATACATT TTCATTTTCT 
ACTGCAAGCG GTAAAAAGGT CAACATAAGC GGAAATTCTT TAAAGCAGGT CAGAGCTGTC 
TGCCTATCCT CTGACCCAAA AGAAACATCA GCGGCTCTGT TTAATGTTGA AAAAAGTGTT 
TTTAATGAAG ATGTTAAGGA TGTATCTTTG CTTCAGCCCA ATGTTACCAT GCCGAAGGCT 
GTGTCGTTTA GTACTGCAAG TGGCAAGACG GTGCAGTTGT CAGACGAGTC TTTGAAGAAA 
GCTAGGGTGA TTTTTTCTGA AATAGATACT TGTCCGTTGA TGCAACAGCA GACTAATGAA 
AGCACTGTTG AAGAGATAGT AATAGGAGGA GGAATGACAA AATCAAAACA GATGCCATTA 
ACAACAGAAA AAGTCGAAAC AACTAGAAAA AATAATGGTA CTTTTGGCTT TAATACCGCA 
AGCGGAAAGC AGGTCTCTGT GTCTGAAAGT GCGCTGCAGA AAGTCAAAGA TATCTTTCAA 
GAATTTGATG ATCCCGACAA TTATGAACAA AATAAATCAC TCGTAAGACT GCCTGTTTCA 
TCAAAAATAA AAGAATCAAC TCCAGGAACA AAAAGACTGG TGCAAACTGC AGGGTCCTCT 
TATAAAAATG ATAATTTGCA ATGTAAAGCA GGGAATCTTC GTACTTTTCA GGATAAGCAA 
GCTGGCAAGA AGTCACTTAC ATATTCAGAA GCAGCCATAA GCCCCATTAT AACAACTGTC 
CCTGGAAATC AATTAACGCC CATCCAGTTG GTAAAAGTCT CAACCAACAC ATCAGCATTG 
GCTAATAAAA CCAAACCCGA TTTATATGTC GCAACAGCCC AGAACACACC ACAAAATGAT 
TTTGAAATAG AAGCAGCAGA AAGTGCTAGA GCTTTCTTGG AAGATGATGA CCTGACAGAT 
ATGGGATCAG CTGCCCATGA AACATACTTG TATCTCAACC AGACCTCAAA TACAAGAACT 
GGGAAGAGAT CAAGAACAGA AGGGCCGACA CCTGGAGAGC CACCAATCAA AAGACGGCTT 
TTACCAGAAT TTGATTGCAC AACAGACACT CGGCAACAAA CGGGGATTTT AAAGCCTTTG 
ATCAGCACTC CAGACATGAT GCGAGACAGG CGAAGGTTTT TGTACACCCT TCCCTTGAAG 
CCATCAACTT GTAATCCTGA AAGTGCTTTA CGTCAAGTCA CGTGCATTCC TACACAAGCC 
CATCTCCATT CTAAAGTCAA AATCTTCCAC CAGTCTCTAC CCATCAAGTC ACCAGATGTT 
GCATCTGATT CAACTTCTAA ATCGTATAGC CCCACTGCTG CAAAAGAGAC CATAAACTGC 
TCATCTGCAT CTAAAATACC TGCAAAGAAA TTTGTGCCTC CTTTTAAAAA GACTGTGGCG 
ACCTTGGCAG ACAACCAGTC AAACAGTGTA CAAAATGGCA GTTCGGATGG GCTTATCGAG 
AGCATTGTTT ATCCAAAAGA AGACAAAGTT GAAACCATAT GTTCTTCAAA AGATCAATTT 
GATGACTCAG ATATTTTACA AATGACTTCA AATCTTCGAT GTAGCAAAGA CCTGCAGGAA 
ATGAGAATTC GGAAAAAGCT AAGGCAAAAG ATTAAACCGC ATCCAGGCTC ACTGTATCGT 
CTGAAAATGT CACATGTGAA GAGAATTTCA CTTCAATCTG CAGTGGCAGA AAGGTGTCCC 
ACTCTGTATT CAAGGGAACA GTTATACAGA TATGGCATTG TGAAGAACCA TATTGGAGTC 
AGCAGCGAGA ACGCATTGTC CTTCCAGTTC CACTGTTCAA ATTATTTCAC AAAAGAACTT 
CTTCTTTCTG GAAATGGTGT GCAGTTGGCA GATGGAGGTT GGCTTATTCC CACTGAACAA 
GGCAACGCAG GCAAAGAAGA GATATACAGA GCGTTTTGTG ACACTCCTGG TGTGGATCCA 
AAATTAATTT CAGCTGAATG GGTTCATAAC CACTACCGCT GGATTGTATG GAAACTTGCT 
GCAATGGAAG TCAGGTTCCC AAAGACATTT GCTTGCAGAT GCCTTACACC AGAACGTGTG 
CTTTTACAGT TAAAGTACAG ATATGATGTG GAAATTGATA AGAGTCAGAG ATCCGCCATC 
AAAAAGATCA TGGAGAGAGA TGACTCCCCA GCTAAAACGC TGGTACTGTG CATTGCCAAG 
ATCATTTCCC AGGGCACTCG CCTGCCCAAT GCATGTAGTA ATAAAACCGA ACCCGCTGAC 
TCTAAGGAAA GTTCTGCAGT AATAGAGGTG ACAGATAGTT GGTATGGTAT CAAGGTTCTG 
CTTGATCCGT GTCTCACTGC TCTCTTACAC AAAGGAAGGC TTTTTATTGG TCAGAAGCTA 
ATTGTTCATG GAGCGGAACT TATTGGCTCA GATGATGCCT GCTCTCCCCT TGAAGCGCCA 
GAATCTCTTA TGTTGAAGAT TGCAGCTAAC AGCACTCGGC CTGTTCGATG GCATACCAAA 
CTTGGATATT TCAAGGATCC CAGACCATTC TGTCTCCATT TATCATCACT GTTAAGTGAA 
GGAGGTGTGG TGGGGTGTGT CGATGTAGTA ATACAAAGAA TCTATCCCAT GCAGTGGATG 
GAAAAAATGG CAAATGGACT GTATGTGTTT CGCAATGATC GAGCAGAGGA AAGAGAAGCA 
GAAAAGCATT CAGCAAATCA ACAAAAAAAG CTTGAAATGC TCTTTTCAAA AATACAAGCT 
GAATTTGAAC AGCGGGAAGT AACATGCAAC AGAAGAAAGG GACTCAGGCG GCGGTCATTA 
AATGCACAAC AAATGCAGAC CCTTCAGGAT GGGGCTGAAA TCTATGAAGC CATTCAGAAC 
GAATCTGACC CTGGGTATCT GGAGTCGTAC TTAAGTGCTG AGCAGCTGAA AGCCCTCAAT 
CACCACAGGC AACTTTTAAA CGATAAGAAG CAAGCACTGA TCCAAGCTGA ATTCAGAAAA 
GCCATTGAAT GCAGTGAGCA GGATGCAAAT GGCTGTACAA GGAGGGATGT CACTCCTGTA 
TGGAAATTAC GCATTGCAGA TTACAGAAAT TATGAAACAG ATGCAGCATA TATACTGAAC 
ATTTGGCGTC CGTTACCAGA TGTGCTTTCT CTTCTGAAAG AAGGTTGTCG CTATAAAATG 
TATCATTTGG CAGCATCCAC ATCAAAAGGA AAGTCATTAG CAGCTGATTT GCAGTTAACA 
GCTACAAAGA AAACTCGCTT CCAGCAACTT CAGCCATCAG AGAGCATTTT GGAGCAGATT 
TACAGCCCGA GAGAAGTGAC GGACTTCAGC AGGTTCCAGG AGCCTTTGTT TAGCGCCCCA 
TATGCAGAGG TTGATCTGGT GGGATTAATC ATCTCCATAT ACAAAAAAAC AGGTGCAGCT 
CCAGTGGTTT ATATATCAGA TGAATCTCAC AATATTGTGG CGCTAAAATT CTGGACAGAT 
CTAGGGCAGC TTGGGCTTGA GGAGATAACC AAGCCACGCA CATATATCTC TGCCAGTAAT 
CTTCGCTGGA GATCAGACTG TATCGAGGGG ATTCCTACAC TGTACGTTGG AGACTTAGCC 
AACATTTCTT CCAATCCCAA AGAAAGCCAT CTGCAGAGGG CAATTCAGAA ACTGAAACTT 
TCTGTACAGA ATGTCCAAGA TTTTTGGAAT TCATCACAAA CCGCACTGAT GAAAACCTTG 
CAGATAAATA GCACAGACAC AACGGAGTGC AGTAAGAACC CAACGACACC AACATGGAAG 
TCAGATGTTT CTGCTAGAAG TGGTTACCTA ACCCCCTTGC ACCATAGTGG TAAAAGGTTA 
TTAAATTCTG TCCATACATC AGATCCTCAG ACAGAAAATC CTGGATGTAG TAAAGAAATA 
CAACTAAAAA CCTGCAAAAA GAGGAAAGCT CTGGACTTTC TCAACCGCAT TCCTTCACCT 
CCACCTGTAA CACCAGTACG GCCCTTTGTA TCCCCATCTT TGCAGAAGGC ATTCCGACCC 
CCACGGAGCT GTAGCGTCCA GAAGTTGGGC CCGGAAACCA AGGGTAATAC TGAGAATGTC 
CAGGGAACAA CCCCAGAATG CACTAAAGAC CTAGCAAAAT TAGAGGGTGA ATTTGTGGCT 
GATGAAGAAC TCGCCATGAT CAATACCCAA GCTTTGCTTT TAGGTCTGGA GGAAGAAAAA 
AAGAAAACCG AGCAGAAAAC CAGCAGAACA GCAGGTAAAA TGACTGCCCA TGAGTCTCCA 
ATAGAAAATG CTTCCCCCGT TCCTGCCCAG GAACAACAAA CTGAGGAAGC ATTGAACATT 
CCAGTCGGTA ACTCAGAGAA ATCTTACCTG TGCCTAAGAA AAAGGAAACG GAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031753094.1
CDS	281..9997
Translation	MAAPQLGKSVFYDLFSTHCSHSDLGPISLNWFEELTAEALPYKSRTCEDHECSLDDLHENNIKTPKLKSSIYSQLDSTPVIFKERLLSPLFASSLTELDKRQNATDNGNNVRLEKSNCTQMQHQASEVFSPSSRCLNESPAVIKEIFKTPLRNKYLHKTPQCDWKPDICSSLFCTPKLMKNQTGCIKESLGAEVDPEMSWSSSLATPPSPTVIIAHANDQPSGNKHAAIVQSLFPNCENMEANNLPPPETGTQNEDRQICGMGSVIQKIPERSSSIDSIITGLEKPGSCSASKTVPEASAISSRAAWKKTVANTVKDEEVSRTVENALEGMEDVLSIFFASEKTPGLRKLKNSTQARRKVESIKSQKCQVSLFKAEEGESILVPLDEHHKCDHEMLMKPEQDYKMNAVNQKIAKETTPYEWSQLNICELDITQSQDSTLHAANLCCEDITVNKSDTCNITEAEKQEEVNEPSTDNVLSNKVGKESTLNVNCINADLNNMASSSNNSVHSLNLSQCEKMDSSEISNSEVGCITKLTTHPAKITTGELSSVDDCAKKPQERVTISTSFSTLKKQSKFMYSVNTVLTGHIASNTSIATRRSLNSHLSSDEPKPHIKENQNEPESNAKYYSNGLQKPVFLEKENDNKGISLCKTISDGQNTSEGARAPDYFEERVPETKDRCKATLSIREKVVATANCFARKQLETDYPEDASAQHEALQFHAKQYVSCSMLNSNVSNDMQIKPQVLTQACDFRSSAENLVEKTKNQLRRNEDQSFSSTGIGKKPASGEKLVGDCNEGSFLHVKQEISAAQALVGNGYETRDTPDVTSDHTRSVISNHGKSLWEEETALTELPVPEILSKTFKGFKTASNKKIHISDKNIVKGCDLFKEIEFGIGDAKPCNEENKEPLKKTPFDVPGYETNLKGFKTASNKEINVSENKFAKGRLLFKDIEEESGQTTAIMAKDNELFIKPASSNKPDIFKSTCIKGMNPSENLDSMPPKKGDVQQSAGLKHSDQIDVFGFDDPPAKGNVQTLGYFNMSASETDFKGFKTASNKDIIISESTLAKGKLIFEDIEDTRYSETGRTIDCKAKGGALSTSNLVMHNTMCKNEPSTSENTNDKAPKRAKVQTPMAENSHLKEPSSDKNAMDAANHQMTLKASAYSPKAASSALPRYKKKPISTTKTSFQLNEHLTESQQAEISELSSILENADSQFDFTQFRKVPSVTEKQNSTEGGSESQNLNNSDVWKDVDFNDSFAAGRDHSEGMEAIPSSPGIKELPSAGECCSKETHDSMPDFVTLAPKQGFLVKQNERLFAGFNLASGKQVNIDNDVLKKAAELFNDIDNDKELLSHAKEESRKSNIKHSSKLINNENCGKTEHTSEHLVCQSNVSLPFIKTLGKNATILVSGNEKSQNQELQLDCLKCESTDVKHTPQKETINDNSKCNESSLSELSMKGFQTASGRNIMMSESSIQKARNIFAEEHEDSFTLRCNIQNTIQIPQPVNEPTQFPYVNLGPKPTTTSGWQEKNILRRSTEKGFMPGFCTAGGKKVSVSDDSLAKAHKLFQEECTFSKEGKLDEVKQNKLMNSEPLSLLTCESVLKQSDGFIEDISTSRNALEIRPELYPEGMCSNRASNGSGNNSEFTAGEGISININQSSLLTTGNVLKNLPSESSGHDVYSVTEHLSTVVKVKRYNDSGHFVNQNLAECNDNHVLSTQKNTANISNRNEDCTSLAPLSFSTASGKSVTVSHDSLQKARLMLSEAANDVTVDTSKQEAAYITPAIRKTEAEKEQNTVDDSDRVNANTFSFSTASGKKVNISGNSLKQVRAVCLSSDPKETSAALFNVEKSVFNEDVKDVSLLQPNVTMPKAVSFSTASGKTVQLSDESLKKARVIFSEIDTCPLMQQQTNESTVEEIVIGGGMTKSKQMPLTTEKVETTRKNNGTFGFNTASGKQVSVSESALQKVKDIFQEFDDPDNYEQNKSLVRLPVSSKIKESTPGTKRLVQTAGSSYKNDNLQCKAGNLRTFQDKQAGKKSLTYSEAAISPIITTVPGNQLTPIQLVKVSTNTSALANKTKPDLYVATAQNTPQNDFEIEAAESARAFLEDDDLTDMGSAAHETYLYLNQTSNTRTGKRSRTEGPTPGEPPIKRRLLPEFDCTTDTRQQTGILKPLISTPDMMRDRRRFLYTLPLKPSTCNPESALRQVTCIPTQAHLHSKVKIFHQSLPIKSPDVASDSTSKSYSPTAAKETINCSSASKIPAKKFVPPFKKTVATLADNQSNSVQNGSSDGLIESIVYPKEDKVETICSSKDQFDDSDILQMTSNLRCSKDLQEMRIRKKLRQKIKPHPGSLYRLKMSHVKRISLQSAVAERCPTLYSREQLYRYGIVKNHIGVSSENALSFQFHCSNYFTKELLLSGNGVQLADGGWLIPTEQGNAGKEEIYRAFCDTPGVDPKLISAEWVHNHYRWIVWKLAAMEVRFPKTFACRCLTPERVLLQLKYRYDVEIDKSQRSAIKKIMERDDSPAKTLVLCIAKIISQGTRLPNACSNKTEPADSKESSAVIEVTDSWYGIKVLLDPCLTALLHKGRLFIGQKLIVHGAELIGSDDACSPLEAPESLMLKIAANSTRPVRWHTKLGYFKDPRPFCLHLSSLLSEGGVVGCVDVVIQRIYPMQWMEKMANGLYVFRNDRAEEREAEKHSANQQKKLEMLFSKIQAEFEQREVTCNRRKGLRRRSLNAQQMQTLQDGAEIYEAIQNESDPGYLESYLSAEQLKALNHHRQLLNDKKQALIQAEFRKAIECSEQDANGCTRRDVTPVWKLRIADYRNYETDAAYILNIWRPLPDVLSLLKEGCRYKMYHLAASTSKGKSLAADLQLTATKKTRFQQLQPSESILEQIYSPREVTDFSRFQEPLFSAPYAEVDLVGLIISIYKKTGAAPVVYISDESHNIVALKFWTDLGQLGLEEITKPRTYISASNLRWRSDCIEGIPTLYVGDLANISSNPKESHLQRAIQKLKLSVQNVQDFWNSSQTALMKTLQINSTDTTECSKNPTTPTWKSDVSARSGYLTPLHHSGKRLLNSVHTSDPQTENPGCSKEIQLKTCKKRKALDFLNRIPSPPPVTPVRPFVSPSLQKAFRPPRSCSVQKLGPETKGNTENVQGTTPECTKDLAKLEGEFVADEELAMINTQALLLGLEEEKKKTEQKTSRTAGKMTAHESPIENASPVPAQEQQTEEALNIPVGNSEKSYLCLRKRKRK

Target ORF information:

RefSeq Version	XM_031897234.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis BRCA2, DNA repair associated (brca2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031897234.1

ORF Insert Sequence:

ATGGCTGCAC CGCAACTTGG AAAATCTGTC TTCTATGATC TGTTTAGCAC GCATTGCTCT 
CACTCAGATT TAGGGCCTAT CAGTCTAAAC TGGTTTGAAG AGCTCACTGC TGAAGCCTTG 
CCTTACAAGT CAAGAACATG TGAAGATCAT GAATGCAGTT TGGATGATCT CCATGAAAAC 
AACATCAAAA CTCCTAAACT GAAGTCGTCA ATTTATAGCC AATTAGATTC CACTCCAGTC 
ATCTTTAAGG AGCGTTTACT ATCACCTCTT TTTGCTTCTT CTTTGACAGA GCTGGATAAA 
AGGCAGAATG CAACAGATAA TGGAAACAAT GTTAGACTTG AGAAAAGTAA CTGTACCCAA 
ATGCAGCATC AAGCAAGTGA AGTTTTCAGT CCCTCAAGTC GATGCCTCAA CGAAAGTCCT 
GCTGTTATCA AAGAAATATT CAAGACACCA TTACGAAATA AGTACCTACA TAAAACACCA 
CAGTGTGATT GGAAACCTGA CATTTGCAGC AGTCTGTTTT GTACTCCAAA GCTTATGAAG 
AACCAAACTG GATGTATTAA AGAAAGTCTA GGTGCAGAGG TGGATCCAGA GATGTCATGG 
TCTAGTTCAC TAGCTACACC TCCTTCCCCA ACTGTGATAA TTGCACATGC TAATGATCAG 
CCCAGTGGAA ATAAACATGC TGCAATTGTA CAGAGCCTTT TCCCAAATTG TGAGAATATG 
GAAGCGAATA ATTTGCCACC ACCTGAGACG GGCACACAAA ATGAAGACCG TCAAATATGT 
GGTATGGGAT CTGTTATCCA GAAAATTCCG GAAAGGTCAT CTTCCATAGA CTCCATTATA 
ACAGGACTAG AAAAGCCAGG TTCATGCAGT GCGAGCAAAA CTGTACCAGA AGCGTCTGCC 
ATTTCCTCAA GGGCAGCCTG GAAGAAAACT GTTGCAAATA CAGTGAAGGA CGAGGAGGTT 
TCCAGAACTG TGGAAAATGC TCTGGAAGGA ATGGAAGATG TCCTCTCAAT CTTTTTTGCA 
AGTGAAAAGA CTCCTGGTCT CAGAAAGCTG AAAAATTCTA CCCAAGCTAG GAGAAAAGTG 
GAATCTATTA AGTCTCAGAA GTGCCAAGTT TCATTATTTA AAGCAGAAGA AGGAGAAAGC 
ATTTTGGTCC CTTTAGATGA GCATCATAAA TGTGATCATG AGATGCTTAT GAAACCTGAA 
CAAGACTACA AAATGAATGC TGTTAACCAA AAAATAGCCA AAGAAACTAC TCCATATGAA 
TGGTCACAGC TAAATATATG TGAGTTGGAT ATAACTCAAT CACAGGATAG CACTTTGCAT 
GCAGCAAATC TATGTTGTGA AGATATCACT GTCAACAAAA GTGATACATG TAATATTACT 
GAAGCCGAAA AGCAGGAGGA AGTCAATGAG CCAAGCACCG ACAATGTACT GTCTAATAAA 
GTTGGCAAAG AAAGCACTTT AAATGTGAAT TGTATTAATG CTGATTTGAA TAACATGGCT 
TCATCCTCAA ATAACTCTGT TCACTCACTG AATCTTTCTC AATGTGAAAA AATGGATTCT 
TCTGAAATTT CTAACAGTGA AGTTGGTTGT ATAACAAAAC TAACAACCCA CCCAGCAAAA 
ATAACAACTG GTGAATTAAG TTCAGTAGAT GACTGTGCTA AGAAACCCCA GGAAAGAGTA 
ACTATTAGTA CTTCATTTTC AACTCTGAAA AAGCAATCGA AGTTTATGTA CAGTGTAAAC 
ACTGTGTTAA CTGGTCATAT TGCAAGCAAT ACCAGCATAG CCACTAGGAG ATCATTGAAC 
AGTCATTTAA GTTCTGATGA ACCAAAACCT CACATTAAGG AAAATCAAAA TGAACCAGAA 
TCAAATGCTA AATATTATTC CAACGGCCTG CAGAAACCAG TATTCCTGGA AAAGGAGAAT 
GATAATAAGG GAATTTCTTT ATGTAAAACT ATCTCAGACG GTCAAAACAC TTCAGAAGGT 
GCCAGGGCTC CAGACTATTT TGAGGAACGT GTTCCTGAAA CAAAAGATAG ATGCAAAGCT 
ACACTAAGCA TCAGGGAAAA GGTTGTGGCT ACAGCAAATT GTTTTGCAAG AAAACAGCTT 
GAAACAGATT ATCCAGAGGA TGCCTCAGCA CAACATGAGG CACTTCAGTT CCATGCTAAG 
CAATATGTGT CATGCTCAAT GCTAAATTCT AATGTGTCCA ATGACATGCA AATAAAGCCT 
CAAGTGTTAA CTCAGGCATG CGATTTTAGG AGCAGCGCAG AAAATCTTGT TGAAAAAACA 
AAAAATCAAT TAAGGCGGAA TGAAGATCAA TCATTTTCAT CAACTGGCAT AGGGAAAAAG 
CCAGCTAGTG GTGAAAAATT AGTAGGTGAC TGTAATGAGG GCTCATTCCT TCATGTGAAG 
CAAGAAATAA GTGCTGCTCA GGCATTGGTA GGTAATGGTT ACGAAACCAG AGACACTCCT 
GATGTCACTA GTGATCATAC AAGGAGTGTT ATTTCCAACC ATGGAAAGTC TCTGTGGGAG 
GAGGAGACAG CACTGACTGA ATTACCAGTC CCAGAAATTC TGAGTAAAAC TTTCAAGGGC 
TTTAAAACGG CATCTAATAA AAAAATACAT ATTTCAGATA AAAATATTGT AAAAGGTTGT 
GACTTGTTCA AAGAAATTGA GTTTGGCATT GGTGACGCTA AGCCCTGCAA TGAAGAAAAC 
AAAGAGCCCT TAAAGAAAAC TCCCTTTGAT GTTCCTGGGT ATGAAACAAA TTTAAAAGGA 
TTCAAAACTG CATCCAATAA AGAAATAAAT GTATCAGAAA ACAAATTTGC TAAAGGTAGA 
CTGCTGTTTA AAGACATCGA AGAGGAAAGC GGTCAAACTA CAGCTATAAT GGCAAAAGAT 
AATGAGCTGT TCATTAAGCC TGCATCCAGC AATAAGCCTG ACATATTTAA GAGTACATGT 
ATAAAAGGAA TGAATCCATC TGAGAACCTA GACAGTATGC CCCCCAAAAA AGGAGACGTG 
CAGCAGAGTG CAGGTTTAAA ACATTCAGAT CAAATTGACG TTTTTGGTTT CGATGATCCT 
CCTGCAAAAG GAAATGTTCA AACATTAGGC TATTTTAATA TGTCTGCTTC TGAAACAGAC 
TTCAAAGGAT TCAAAACCGC TTCCAATAAA GACATAATTA TTTCAGAAAG CACACTTGCT 
AAAGGTAAAC TTATTTTTGA AGATATTGAA GATACAAGAT ACAGTGAAAC AGGAAGGACC 
ATAGATTGTA AAGCAAAAGG TGGTGCGTTG TCCACCAGTA ACCTTGTAAT GCATAACACC 
ATGTGTAAAA ATGAACCAAG TACATCTGAG AACACAAATG ATAAAGCACC AAAAAGAGCA 
AAAGTACAAA CACCTATGGC AGAAAATAGT CATTTAAAAG AACCTTCTTC AGACAAAAAT 
GCAATGGATG CTGCAAATCA TCAAATGACA CTGAAAGCCT CCGCATACAG CCCAAAGGCT 
GCCTCTTCTG CACTGCCCAG GTACAAAAAG AAACCAATCT CTACCACTAA AACCTCTTTC 
CAGTTGAATG AACATTTGAC TGAAAGCCAG CAAGCTGAAA TCTCTGAACT TTCCTCTATT 
CTAGAAAATG CAGACAGTCA GTTTGATTTT ACTCAGTTTA GGAAAGTGCC TTCTGTGACT 
GAGAAACAAA ATTCAACTGA AGGTGGGAGC GAGTCCCAGA ACCTTAACAA CTCAGATGTC 
TGGAAGGACG TAGATTTTAA TGATAGTTTT GCAGCAGGAA GGGATCATTC TGAGGGAATG 
GAGGCAATTC CCTCCAGTCC TGGAATAAAA GAATTGCCTT CTGCAGGAGA ATGTTGCAGT 
AAAGAAACAC ATGATTCTAT GCCAGATTTT GTGACCTTGG CACCTAAACA AGGTTTTCTG 
GTTAAACAAA ATGAAAGACT CTTTGCAGGG TTTAATCTTG CTAGTGGCAA GCAAGTTAAT 
ATAGACAATG ATGTACTAAA AAAAGCTGCT GAACTGTTCA ATGATATAGA TAATGATAAA 
GAATTGCTCA GCCACGCCAA AGAAGAAAGC AGAAAAAGCA ATATAAAACA CTCTTCTAAA 
TTAATAAATA ATGAAAATTG TGGAAAGACA GAACACACAT CTGAGCATTT GGTATGTCAA 
AGTAATGTTT CATTACCATT CATAAAAACA TTGGGCAAGA ATGCTACAAT ACTAGTGAGT 
GGTAATGAAA AAAGCCAGAA TCAAGAATTA CAGCTGGATT GTTTAAAATG TGAAAGTACT 
GATGTAAAGC ATACACCACA AAAGGAAACA ATCAATGACA ACTCAAAGTG TAATGAATCC 
TCTCTTTCTG AGCTTTCCAT GAAAGGTTTT CAGACTGCCA GTGGGAGAAA CATTATGATG 
TCCGAGTCTT CAATCCAGAA AGCCAGAAAC ATATTTGCAG AGGAACATGA AGACAGTTTT 
ACATTGCGCT GTAATATTCA AAATACAATA CAAATACCAC AGCCTGTAAA TGAACCGACC 
CAATTCCCAT ATGTTAATTT GGGGCCAAAA CCCACAACGA CATCAGGATG GCAAGAAAAA 
AATATTCTGA GAAGAAGTAC AGAAAAAGGT TTCATGCCGG GTTTTTGCAC TGCCGGTGGT 
AAAAAGGTTT CTGTTTCCGA CGACTCTCTA GCTAAAGCAC ATAAACTTTT CCAGGAGGAA 
TGTACCTTTA GCAAGGAAGG GAAATTGGAT GAGGTGAAAC AAAATAAATT AATGAACTCT 
GAGCCATTGT CTCTTCTCAC TTGTGAGTCA GTCCTGAAAC AGAGTGACGG ATTCATTGAA 
GATATTTCAA CTTCTAGAAA TGCTTTGGAA ATCAGACCAG AGCTTTATCC GGAGGGCATG 
TGTAGTAATA GGGCATCTAA TGGAAGTGGA AACAATAGTG AATTTACAGC TGGGGAAGGC 
ATATCAATAA ATATCAACCA ATCATCGCTG CTTACAACAG GCAACGTTTT AAAAAATTTG 
CCGTCTGAAA GTAGTGGCCA TGATGTGTAT TCAGTAACTG AGCATCTAAG CACTGTGGTT 
AAAGTCAAAA GATACAATGA TTCAGGTCAT TTTGTAAATC AAAATTTAGC TGAATGTAAT 
GATAACCATG TCCTTAGCAC TCAAAAAAAT ACTGCAAATA TAAGCAACAG AAACGAGGAC 
TGTACATCCC TGGCACCTCT CTCTTTCAGC ACAGCCAGTG GGAAATCCGT CACAGTGTCT 
CATGATTCTC TTCAAAAAGC AAGACTGATG CTTTCTGAAG CAGCTAATGA TGTTACAGTA 
GATACTAGTA AGCAAGAGGC GGCTTATATT ACGCCAGCCA TTCGAAAAAC AGAAGCAGAA 
AAAGAGCAGA ACACTGTTGA TGATTCTGAT AGAGTTAATG CAAATACATT TTCATTTTCT 
ACTGCAAGCG GTAAAAAGGT CAACATAAGC GGAAATTCTT TAAAGCAGGT CAGAGCTGTC 
TGCCTATCCT CTGACCCAAA AGAAACATCA GCGGCTCTGT TTAATGTTGA AAAAAGTGTT 
TTTAATGAAG ATGTTAAGGA TGTATCTTTG CTTCAGCCCA ATGTTACCAT GCCGAAGGCT 
GTGTCGTTTA GTACTGCAAG TGGCAAGACG GTGCAGTTGT CAGACGAGTC TTTGAAGAAA 
GCTAGGGTGA TTTTTTCTGA AATAGATACT TGTCCGTTGA TGCAACAGCA GACTAATGAA 
AGCACTGTTG AAGAGATAGT AATAGGAGGA GGAATGACAA AATCAAAACA GATGCCATTA 
ACAACAGAAA AAGTCGAAAC AACTAGAAAA AATAATGGTA CTTTTGGCTT TAATACCGCA 
AGCGGAAAGC AGGTCTCTGT GTCTGAAAGT GCGCTGCAGA AAGTCAAAGA TATCTTTCAA 
GAATTTGATG ATCCCGACAA TTATGAACAA AATAAATCAC TCGTAAGACT GCCTGTTTCA 
TCAAAAATAA AAGAATCAAC TCCAGGAACA AAAAGACTGG TGCAAACTGC AGGGTCCTCT 
TATAAAAATG ATAATTTGCA ATGTAAAGCA GGGAATCTTC GTACTTTTCA GGATAAGCAA 
GCTGGCAAGA AGTCACTTAC ATATTCAGAA GCAGCCATAA GCCCCATTAT AACAACTGTC 
CCTGGAAATC AATTAACGCC CATCCAGTTG GTAAAAGTCT CAACCAACAC ATCAGCATTG 
GCTAATAAAA CCAAACCCGA TTTATATGTC GCAACAGCCC AGAACACACC ACAAAATGAT 
TTTGAAATAG AAGCAGCAGA AAGTGCTAGA GCTTTCTTGG AAGATGATGA CCTGACAGAT 
ATGGGATCAG CTGCCCATGA AACATACTTG TATCTCAACC AGACCTCAAA TACAAGAACT 
GGGAAGAGAT CAAGAACAGA AGGGCCGACA CCTGGAGAGC CACCAATCAA AAGACGGCTT 
TTACCAGAAT TTGATTGCAC AACAGACACT CGGCAACAAA CGGGGATTTT AAAGCCTTTG 
ATCAGCACTC CAGACATGAT GCGAGACAGG CGAAGGTTTT TGTACACCCT TCCCTTGAAG 
CCATCAACTT GTAATCCTGA AAGTGCTTTA CGTCAAGTCA CGTGCATTCC TACACAAGCC 
CATCTCCATT CTAAAGTCAA AATCTTCCAC CAGTCTCTAC CCATCAAGTC ACCAGATGTT 
GCATCTGATT CAACTTCTAA ATCGTATAGC CCCACTGCTG CAAAAGAGAC CATAAACTGC 
TCATCTGCAT CTAAAATACC TGCAAAGAAA TTTGTGCCTC CTTTTAAAAA GACTGTGGCG 
ACCTTGGCAG ACAACCAGTC AAACAGTGTA CAAAATGGCA GTTCGGATGG GCTTATCGAG 
AGCATTGTTT ATCCAAAAGA AGACAAAGTT GAAACCATAT GTTCTTCAAA AGATCAATTT 
GATGACTCAG ATATTTTACA AATGACTTCA AATCTTCGAT GTAGCAAAGA CCTGCAGGAA 
ATGAGAATTC GGAAAAAGCT AAGGCAAAAG ATTAAACCGC ATCCAGGCTC ACTGTATCGT 
CTGAAAATGT CACATGTGAA GAGAATTTCA CTTCAATCTG CAGTGGCAGA AAGGTGTCCC 
ACTCTGTATT CAAGGGAACA GTTATACAGA TATGGCATTG TGAAGAACCA TATTGGAGTC 
AGCAGCGAGA ACGCATTGTC CTTCCAGTTC CACTGTTCAA ATTATTTCAC AAAAGAACTT 
CTTCTTTCTG GAAATGGTGT GCAGTTGGCA GATGGAGGTT GGCTTATTCC CACTGAACAA 
GGCAACGCAG GCAAAGAAGA GATATACAGA GCGTTTTGTG ACACTCCTGG TGTGGATCCA 
AAATTAATTT CAGCTGAATG GGTTCATAAC CACTACCGCT GGATTGTATG GAAACTTGCT 
GCAATGGAAG TCAGGTTCCC AAAGACATTT GCTTGCAGAT GCCTTACACC AGAACGTGTG 
CTTTTACAGT TAAAGTACAG ATATGATGTG GAAATTGATA AGAGTCAGAG ATCCGCCATC 
AAAAAGATCA TGGAGAGAGA TGACTCCCCA GCTAAAACGC TGGTACTGTG CATTGCCAAG 
ATCATTTCCC AGGGCACTCG CCTGCCCAAT GCATGTAGTA ATAAAACCGA ACCCGCTGAC 
TCTAAGGAAA GTTCTGCAGT AATAGAGGTG ACAGATAGTT GGTATGGTAT CAAGGTTCTG 
CTTGATCCGT GTCTCACTGC TCTCTTACAC AAAGGAAGGC TTTTTATTGG TCAGAAGCTA 
ATTGTTCATG GAGCGGAACT TATTGGCTCA GATGATGCCT GCTCTCCCCT TGAAGCGCCA 
GAATCTCTTA TGTTGAAGAT TGCAGCTAAC AGCACTCGGC CTGTTCGATG GCATACCAAA 
CTTGGATATT TCAAGGATCC CAGACCATTC TGTCTCCATT TATCATCACT GTTAAGTGAA 
GGAGGTGTGG TGGGGTGTGT CGATGTAGTA ATACAAAGAA TCTATCCCAT GCAGTGGATG 
GAAAAAATGG CAAATGGACT GTATGTGTTT CGCAATGATC GAGCAGAGGA AAGAGAAGCA 
GAAAAGCATT CAGCAAATCA ACAAAAAAAG CTTGAAATGC TCTTTTCAAA AATACAAGCT 
GAATTTGAAC AGCGGGAAGT AACATGCAAC AGAAGAAAGG GACTCAGGCG GCGGTCATTA 
AATGCACAAC AAATGCAGAC CCTTCAGGAT GGGGCTGAAA TCTATGAAGC CATTCAGAAC 
GAATCTGACC CTGGGTATCT GGAGTCGTAC TTAAGTGCTG AGCAGCTGAA AGCCCTCAAT 
CACCACAGGC AACTTTTAAA CGATAAGAAG CAAGCACTGA TCCAAGCTGA ATTCAGAAAA 
GCCATTGAAT GCAGTGAGCA GGATGCAAAT GGCTGTACAA GGAGGGATGT CACTCCTGTA 
TGGAAATTAC GCATTGCAGA TTACAGAAAT TATGAAACAG ATGCAGCATA TATACTGAAC 
ATTTGGCGTC CGTTACCAGA TGTGCTTTCT CTTCTGAAAG AAGGTTGTCG CTATAAAATG 
TATCATTTGG CAGCATCCAC ATCAAAAGGA AAGTCATTAG CAGCTGATTT GCAGTTAACA 
GCTACAAAGA AAACTCGCTT CCAGCAACTT CAGCCATCAG AGAGCATTTT GGAGCAGATT 
TACAGCCCGA GAGAAGTGAC GGACTTCAGC AGGTTCCAGG AGCCTTTGTT TAGCGCCCCA 
TATGCAGAGG TTGATCTGGT GGGATTAATC ATCTCCATAT ACAAAAAAAC AGGTGCAGCT 
CCAGTGGTTT ATATATCAGA TGAATCTCAC AATATTGTGG CGCTAAAATT CTGGACAGAT 
CTAGGGCAGC TTGGGCTTGA GGAGATAACC AAGCCACGCA CATATATCTC TGCCAGTAAT 
CTTCGCTGGA GATCAGACTG TATCGAGGGG ATTCCTACAC TGTACGTTGG AGACTTAGCC 
AACATTTCTT CCAATCCCAA AGAAAGCCAT CTGCAGAGGG CAATTCAGAA ACTGAAACTT 
TCTGTACAGA ATGTCCAAGA TTTTTGGAAT TCATCACAAA CCGCACTGAT GAAAACCTTG 
CAGATAAATA GCACAGACAC AACGGAGTGC AGTAAGAACC CAACGACACC AACATGGAAG 
TCAGATGTTT CTGCTAGAAG TGGTTACCTA ACCCCCTTGC ACCATAGTGG TAAAAGGTTA 
TTAAATTCTG TCCATACATC AGATCCTCAG ACAGAAAATC CTGGATGTAG TAAAGAAATA 
CAACTAAAAA CCTGCAAAAA GAGGAAAGCT CTGGACTTTC TCAACCGCAT TCCTTCACCT 
CCACCTGTAA CACCAGTACG GCCCTTTGTA TCCCCATCTT TGCAGAAGGC ATTCCGACCC 
CCACGGAGCT GTAGCGTCCA GAAGTTGGGC CCGGAAACCA AGGGTAATAC TGAGAATGTC 
CAGGGAACAA CCCCAGAATG CACTAAAGAC CTAGCAAAAT TAGAGGGTGA ATTTGTGGCT 
GATGAAGAAC TCGCCATGAT CAATACCCAA GCTTTGCTTT TAGGTCTGGA GGAAGAAAAA 
AAGAAAACCG AGCAGAAAAC CAGCAGAACA GCAGGTAAAA TGACTGCCCA TGAGTCTCCA 
ATAGAAAATG CTTCCCCCGT TCCTGCCCAG GAACAACAAA CTGAGGAAGC ATTGAACATT 
CCAGTCGGTA ACTCAGAGAA ATCTTACCTG TGCCTAAGAA AAAGGAAACG GAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

brca2 ( XM_031897236.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031753096.1 Xenopus tropicalis BRCA2, DNA repair associated (brca2), transcript variant X3, mRNA.

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CloneID	OXa52806
Clone ID Related Accession (Same CDS sequence)	XM_031897236.1
Accession Version	XM_031897236.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	breast cancer type 2 susceptibility protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAC CGCAACTTGG AAAATCTGTC TTCTATGATC TGTTTAGCAC GCATTGCTCT 
CACTCAGATT TAGGGCCTAT CAGTCTAAAC TGGTTTGAAG AGCTCACTGC TGAAGCCTTG 
CCTTACAAGT CAAGAACATG TGAAGATCAT GAATGCAGTT TGGATGATCT CCATGAAAAC 
AACATCAAAA CTCCTAAACT GAAGTCGTCA ATTTATAGCC AATTAGATTC CACTCCAGTC 
ATCTTTAAGG AGCGTTTACT ATCACCTCTT TTTGCTTCTT CTTTGACAGA GCTGGATAAA 
AGGCAGAATG CAACAGATAA TGGAAACAAT GTTAGACTTG AGAAAAGTAA CTGTACCCAA 
ATGCAGCATC AAGCAAGTGA AGTTTTCAGT CCCTCAAGTC GATGCCTCAA CGAAAGTCCT 
GCTGTTATCA AAGAAATATT CAAGACACCA TTACGAAATA AGTACCTACA TAAAACACCA 
CAGTGTGATT GGAAACCTGA CATTTGCAGC AGTCTGTTTT GTACTCCAAA GCTTATGAAG 
AACCAAACTG GATGTATTAA AGAAAGTCTA GGTGCAGAGG TGGATCCAGA GATGTCATGG 
TCTAGTTCAC TAGCTACACC TCCTTCCCCA ACTGTGATAA TTGCACATGC TAATGATCAG 
CCCAGTGGAA ATAAACATGC TGCAATTGTA CAGAGCCTTT TCCCAAATTG TGAGAATATG 
GAAGCGAATA ATTTGCCACC ACCTGAGACG GGCACACAAA ATGAAGACCG TCAAATATGT 
GGTATGGGAT CTGTTATCCA GAAAATTCCG GAAAGGTCAT CTTCCATAGA CTCCATTATA 
AGACTAGAAA AGCCAGGTTC ATGCAGTGCG AGCAAAACTG TACCAGAAGC GTCTGCCATT 
TCCTCAAGGG CAGCCTGGAA GAAAACTGTT GCAAATACAG TGAAGGACGA GGAGGTTTCC 
AGAACTGTGG AAAATGCTCT GGAAGGAATG GAAGATGTCC TCTCAATCTT TTTTGCAAGT 
GAAAAGACTC CTGGTCTCAG AAAGCTGAAA AATTCTACCC AAGCTAGGAG AAAAGTGGAA 
TCTATTAAGT CTCAGAAGTG CCAAGTTTCA TTATTTAAAG CAGAAGAAGG AGAAAGCATT 
TTGGTCCCTT TAGATGAGCA TCATAAATGT GATCATGAGA TGCTTATGAA ACCTGAACAA 
GACTACAAAA TGAATGCTGT TAACCAAAAA ATAGCCAAAG AAACTACTCC ATATGAATGG 
TCACAGCTAA ATATATGTGA GTTGGATATA ACTCAATCAC AGGATAGCAC TTTGCATGCA 
GCAAATCTAT GTTGTGAAGA TATCACTGTC AACAAAAGTG ATACATGTAA TATTACTGAA 
GCCGAAAAGC AGGAGGAAGT CAATGAGCCA AGCACCGACA ATGTACTGTC TAATAAAGTT 
GGCAAAGAAA GCACTTTAAA TGTGAATTGT ATTAATGCTG ATTTGAATAA CATGGCTTCA 
TCCTCAAATA ACTCTGTTCA CTCACTGAAT CTTTCTCAAT GTGAAAAAAT GGATTCTTCT 
GAAATTTCTA ACAGTGAAGT TGGTTGTATA ACAAAACTAA CAACCCACCC AGCAAAAATA 
ACAACTGGTG AATTAAGTTC AGTAGATGAC TGTGCTAAGA AACCCCAGGA AAGAGTAACT 
ATTAGTACTT CATTTTCAAC TCTGAAAAAG CAATCGAAGT TTATGTACAG TGTAAACACT 
GTGTTAACTG GTCATATTGC AAGCAATACC AGCATAGCCA CTAGGAGATC ATTGAACAGT 
CATTTAAGTT CTGATGAACC AAAACCTCAC ATTAAGGAAA ATCAAAATGA ACCAGAATCA 
AATGCTAAAT ATTATTCCAA CGGCCTGCAG AAACCAGTAT TCCTGGAAAA GGAGAATGAT 
AATAAGGGAA TTTCTTTATG TAAAACTATC TCAGACGGTC AAAACACTTC AGAAGGTGCC 
AGGGCTCCAG ACTATTTTGA GGAACGTGTT CCTGAAACAA AAGATAGATG CAAAGCTACA 
CTAAGCATCA GGGAAAAGGT TGTGGCTACA GCAAATTGTT TTGCAAGAAA ACAGCTTGAA 
ACAGATTATC CAGAGGATGC CTCAGCACAA CATGAGGCAC TTCAGTTCCA TGCTAAGCAA 
TATGTGTCAT GCTCAATGCT AAATTCTAAT GTGTCCAATG ACATGCAAAT AAAGCCTCAA 
GTGTTAACTC AGGCATGCGA TTTTAGGAGC AGCGCAGAAA ATCTTGTTGA AAAAACAAAA 
AATCAATTAA GGCGGAATGA AGATCAATCA TTTTCATCAA CTGGCATAGG GAAAAAGCCA 
GCTAGTGGTG AAAAATTAGT AGGTGACTGT AATGAGGGCT CATTCCTTCA TGTGAAGCAA 
GAAATAAGTG CTGCTCAGGC ATTGGTAGGT AATGGTTACG AAACCAGAGA CACTCCTGAT 
GTCACTAGTG ATCATACAAG GAGTGTTATT TCCAACCATG GAAAGTCTCT GTGGGAGGAG 
GAGACAGCAC TGACTGAATT ACCAGTCCCA GAAATTCTGA GTAAAACTTT CAAGGGCTTT 
AAAACGGCAT CTAATAAAAA AATACATATT TCAGATAAAA ATATTGTAAA AGGTTGTGAC 
TTGTTCAAAG AAATTGAGTT TGGCATTGGT GACGCTAAGC CCTGCAATGA AGAAAACAAA 
GAGCCCTTAA AGAAAACTCC CTTTGATGTT CCTGGGTATG AAACAAATTT AAAAGGATTC 
AAAACTGCAT CCAATAAAGA AATAAATGTA TCAGAAAACA AATTTGCTAA AGGTAGACTG 
CTGTTTAAAG ACATCGAAGA GGAAAGCGGT CAAACTACAG CTATAATGGC AAAAGATAAT 
GAGCTGTTCA TTAAGCCTGC ATCCAGCAAT AAGCCTGACA TATTTAAGAG TACATGTATA 
AAAGGAATGA ATCCATCTGA GAACCTAGAC AGTATGCCCC CCAAAAAAGG AGACGTGCAG 
CAGAGTGCAG GTTTAAAACA TTCAGATCAA ATTGACGTTT TTGGTTTCGA TGATCCTCCT 
GCAAAAGGAA ATGTTCAAAC ATTAGGCTAT TTTAATATGT CTGCTTCTGA AACAGACTTC 
AAAGGATTCA AAACCGCTTC CAATAAAGAC ATAATTATTT CAGAAAGCAC ACTTGCTAAA 
GGTAAACTTA TTTTTGAAGA TATTGAAGAT ACAAGATACA GTGAAACAGG AAGGACCATA 
GATTGTAAAG CAAAAGGTGG TGCGTTGTCC ACCAGTAACC TTGTAATGCA TAACACCATG 
TGTAAAAATG AACCAAGTAC ATCTGAGAAC ACAAATGATA AAGCACCAAA AAGAGCAAAA 
GTACAAACAC CTATGGCAGA AAATAGTCAT TTAAAAGAAC CTTCTTCAGA CAAAAATGCA 
ATGGATGCTG CAAATCATCA AATGACACTG AAAGCCTCCG CATACAGCCC AAAGGCTGCC 
TCTTCTGCAC TGCCCAGGTA CAAAAAGAAA CCAATCTCTA CCACTAAAAC CTCTTTCCAG 
TTGAATGAAC ATTTGACTGA AAGCCAGCAA GCTGAAATCT CTGAACTTTC CTCTATTCTA 
GAAAATGCAG ACAGTCAGTT TGATTTTACT CAGTTTAGGA AAGTGCCTTC TGTGACTGAG 
AAACAAAATT CAACTGAAGG TGGGAGCGAG TCCCAGAACC TTAACAACTC AGATGTCTGG 
AAGGACGTAG ATTTTAATGA TAGTTTTGCA GCAGGAAGGG ATCATTCTGA GGGAATGGAG 
GCAATTCCCT CCAGTCCTGG AATAAAAGAA TTGCCTTCTG CAGGAGAATG TTGCAGTAAA 
GAAACACATG ATTCTATGCC AGATTTTGTG ACCTTGGCAC CTAAACAAGG TTTTCTGGTT 
AAACAAAATG AAAGACTCTT TGCAGGGTTT AATCTTGCTA GTGGCAAGCA AGTTAATATA 
GACAATGATG TACTAAAAAA AGCTGCTGAA CTGTTCAATG ATATAGATAA TGATAAAGAA 
TTGCTCAGCC ACGCCAAAGA AGAAAGCAGA AAAAGCAATA TAAAACACTC TTCTAAATTA 
ATAAATAATG AAAATTGTGG AAAGACAGAA CACACATCTG AGCATTTGGT ATGTCAAAGT 
AATGTTTCAT TACCATTCAT AAAAACATTG GGCAAGAATG CTACAATACT AGTGAGTGGT 
AATGAAAAAA GCCAGAATCA AGAATTACAG CTGGATTGTT TAAAATGTGA AAGTACTGAT 
GTAAAGCATA CACCACAAAA GGAAACAATC AATGACAACT CAAAGTGTAA TGAATCCTCT 
CTTTCTGAGC TTTCCATGAA AGGTTTTCAG ACTGCCAGTG GGAGAAACAT TATGATGTCC 
GAGTCTTCAA TCCAGAAAGC CAGAAACATA TTTGCAGAGG AACATGAAGA CAGTTTTACA 
TTGCGCTGTA ATATTCAAAA TACAATACAA ATACCACAGC CTGTAAATGA ACCGACCCAA 
TTCCCATATG TTAATTTGGG GCCAAAACCC ACAACGACAT CAGGATGGCA AGAAAAAAAT 
ATTCTGAGAA GAAGTACAGA AAAAGGTTTC ATGCCGGGTT TTTGCACTGC CGGTGGTAAA 
AAGGTTTCTG TTTCCGACGA CTCTCTAGCT AAAGCACATA AACTTTTCCA GGAGGAATGT 
ACCTTTAGCA AGGAAGGGAA ATTGGATGAG GTGAAACAAA ATAAATTAAT GAACTCTGAG 
CCATTGTCTC TTCTCACTTG TGAGTCAGTC CTGAAACAGA GTGACGGATT CATTGAAGAT 
ATTTCAACTT CTAGAAATGC TTTGGAAATC AGACCAGAGC TTTATCCGGA GGGCATGTGT 
AGTAATAGGG CATCTAATGG AAGTGGAAAC AATAGTGAAT TTACAGCTGG GGAAGGCATA 
TCAATAAATA TCAACCAATC ATCGCTGCTT ACAACAGGCA ACGTTTTAAA AAATTTGCCG 
TCTGAAAGTA GTGGCCATGA TGTGTATTCA GTAACTGAGC ATCTAAGCAC TGTGGTTAAA 
GTCAAAAGAT ACAATGATTC AGGTCATTTT GTAAATCAAA ATTTAGCTGA ATGTAATGAT 
AACCATGTCC TTAGCACTCA AAAAAATACT GCAAATATAA GCAACAGAAA CGAGGACTGT 
ACATCCCTGG CACCTCTCTC TTTCAGCACA GCCAGTGGGA AATCCGTCAC AGTGTCTCAT 
GATTCTCTTC AAAAAGCAAG ACTGATGCTT TCTGAAGCAG CTAATGATGT TACAGTAGAT 
ACTAGTAAGC AAGAGGCGGC TTATATTACG CCAGCCATTC GAAAAACAGA AGCAGAAAAA 
GAGCAGAACA CTGTTGATGA TTCTGATAGA GTTAATGCAA ATACATTTTC ATTTTCTACT 
GCAAGCGGTA AAAAGGTCAA CATAAGCGGA AATTCTTTAA AGCAGGTCAG AGCTGTCTGC 
CTATCCTCTG ACCCAAAAGA AACATCAGCG GCTCTGTTTA ATGTTGAAAA AAGTGTTTTT 
AATGAAGATG TTAAGGATGT ATCTTTGCTT CAGCCCAATG TTACCATGCC GAAGGCTGTG 
TCGTTTAGTA CTGCAAGTGG CAAGACGGTG CAGTTGTCAG ACGAGTCTTT GAAGAAAGCT 
AGGGTGATTT TTTCTGAAAT AGATACTTGT CCGTTGATGC AACAGCAGAC TAATGAAAGC 
ACTGTTGAAG AGATAGTAAT AGGAGGAGGA ATGACAAAAT CAAAACAGAT GCCATTAACA 
ACAGAAAAAG TCGAAACAAC TAGAAAAAAT AATGGTACTT TTGGCTTTAA TACCGCAAGC 
GGAAAGCAGG TCTCTGTGTC TGAAAGTGCG CTGCAGAAAG TCAAAGATAT CTTTCAAGAA 
TTTGATGATC CCGACAATTA TGAACAAAAT AAATCACTCG TAAGACTGCC TGTTTCATCA 
AAAATAAAAG AATCAACTCC AGGAACAAAA AGACTGGTGC AAACTGCAGG GTCCTCTTAT 
AAAAATGATA ATTTGCAATG TAAAGCAGGG AATCTTCGTA CTTTTCAGGA TAAGCAAGCT 
GGCAAGAAGT CACTTACATA TTCAGAAGCA GCCATAAGCC CCATTATAAC AACTGTCCCT 
GGAAATCAAT TAACGCCCAT CCAGTTGGTA AAAGTCTCAA CCAACACATC AGCATTGGCT 
AATAAAACCA AACCCGATTT ATATGTCGCA ACAGCCCAGA ACACACCACA AAATGATTTT 
GAAATAGAAG CAGCAGAAAG TGCTAGAGCT TTCTTGGAAG ATGATGACCT GACAGATATG 
GGATCAGCTG CCCATGAAAC ATACTTGTAT CTCAACCAGA CCTCAAATAC AAGAACTGGG 
AAGAGATCAA GAACAGAAGG GCCGACACCT GGAGAGCCAC CAATCAAAAG ACGGCTTTTA 
CCAGAATTTG ATTGCACAAC AGACACTCGG CAACAAACGG GGATTTTAAA GCCTTTGATC 
AGCACTCCAG ACATGATGCG AGACAGGCGA AGGTTTTTGT ACACCCTTCC CTTGAAGCCA 
TCAACTTGTA ATCCTGAAAG TGCTTTACGT CAAGTCACGT GCATTCCTAC ACAAGCCCAT 
CTCCATTCTA AAGTCAAAAT CTTCCACCAG TCTCTACCCA TCAAGTCACC AGATGTTGCA 
TCTGATTCAA CTTCTAAATC GTATAGCCCC ACTGCTGCAA AAGAGACCAT AAACTGCTCA 
TCTGCATCTA AAATACCTGC AAAGAAATTT GTGCCTCCTT TTAAAAAGAC TGTGGCGACC 
TTGGCAGACA ACCAGTCAAA CAGTGTACAA AATGGCAGTT CGGATGGGCT TATCGAGAGC 
ATTGTTTATC CAAAAGAAGA CAAAGTTGAA ACCATATGTT CTTCAAAAGA TCAATTTGAT 
GACTCAGATA TTTTACAAAT GACTTCAAAT CTTCGATGTA GCAAAGACCT GCAGGAAATG 
AGAATTCGGA AAAAGCTAAG GCAAAAGATT AAACCGCATC CAGGCTCACT GTATCGTCTG 
AAAATGTCAC ATGTGAAGAG AATTTCACTT CAATCTGCAG TGGCAGAAAG GTGTCCCACT 
CTGTATTCAA GGGAACAGTT ATACAGATAT GGCATTGTGA AGAACCATAT TGGAGTCAGC 
AGCGAGAACG CATTGTCCTT CCAGTTCCAC TGTTCAAATT ATTTCACAAA AGAACTTCTT 
CTTTCTGGAA ATGGTGTGCA GTTGGCAGAT GGAGGTTGGC TTATTCCCAC TGAACAAGGC 
AACGCAGGCA AAGAAGAGAT ATACAGAGCG TTTTGTGACA CTCCTGGTGT GGATCCAAAA 
TTAATTTCAG CTGAATGGGT TCATAACCAC TACCGCTGGA TTGTATGGAA ACTTGCTGCA 
ATGGAAGTCA GGTTCCCAAA GACATTTGCT TGCAGATGCC TTACACCAGA ACGTGTGCTT 
TTACAGTTAA AGTACAGATA TGATGTGGAA ATTGATAAGA GTCAGAGATC CGCCATCAAA 
AAGATCATGG AGAGAGATGA CTCCCCAGCT AAAACGCTGG TACTGTGCAT TGCCAAGATC 
ATTTCCCAGG GCACTCGCCT GCCCAATGCA TGTAGTAATA AAACCGAACC CGCTGACTCT 
AAGGAAAGTT CTGCAGTAAT AGAGGTGACA GATAGTTGGT ATGGTATCAA GGTTCTGCTT 
GATCCGTGTC TCACTGCTCT CTTACACAAA GGAAGGCTTT TTATTGGTCA GAAGCTAATT 
GTTCATGGAG CGGAACTTAT TGGCTCAGAT GATGCCTGCT CTCCCCTTGA AGCGCCAGAA 
TCTCTTATGT TGAAGATTGC AGCTAACAGC ACTCGGCCTG TTCGATGGCA TACCAAACTT 
GGATATTTCA AGGATCCCAG ACCATTCTGT CTCCATTTAT CATCACTGTT AAGTGAAGGA 
GGTGTGGTGG GGTGTGTCGA TGTAGTAATA CAAAGAATCT ATCCCATGCA GTGGATGGAA 
AAAATGGCAA ATGGACTGTA TGTGTTTCGC AATGATCGAG CAGAGGAAAG AGAAGCAGAA 
AAGCATTCAG CAAATCAACA AAAAAAGCTT GAAATGCTCT TTTCAAAAAT ACAAGCTGAA 
TTTGAACAGC GGGAAGTAAC ATGCAACAGA AGAAAGGGAC TCAGGCGGCG GTCATTAAAT 
GCACAACAAA TGCAGACCCT TCAGGATGGG GCTGAAATCT ATGAAGCCAT TCAGAACGAA 
TCTGACCCTG GGTATCTGGA GTCGTACTTA AGTGCTGAGC AGCTGAAAGC CCTCAATCAC 
CACAGGCAAC TTTTAAACGA TAAGAAGCAA GCACTGATCC AAGCTGAATT CAGAAAAGCC 
ATTGAATGCA GTGAGCAGGA TGCAAATGGC TGTACAAGGA GGGATGTCAC TCCTGTATGG 
AAATTACGCA TTGCAGATTA CAGAAATTAT GAAACAGATG CAGCATATAT ACTGAACATT 
TGGCGTCCGT TACCAGATGT GCTTTCTCTT CTGAAAGAAG GTTGTCGCTA TAAAATGTAT 
CATTTGGCAG CATCCACATC AAAAGGAAAG TCATTAGCAG CTGATTTGCA GTTAACAGCT 
ACAAAGAAAA CTCGCTTCCA GCAACTTCAG CCATCAGAGA GCATTTTGGA GCAGATTTAC 
AGCCCGAGAG AAGTGACGGA CTTCAGCAGG TTCCAGGAGC CTTTGTTTAG CGCCCCATAT 
GCAGAGGTTG ATCTGGTGGG ATTAATCATC TCCATATACA AAAAAACAGG TGCAGCTCCA 
GTGGTTTATA TATCAGATGA ATCTCACAAT ATTGTGGCGC TAAAATTCTG GACAGATCTA 
GGGCAGCTTG GGCTTGAGGA GATAACCAAG CCACGCACAT ATATCTCTGC CAGTAATCTT 
CGCTGGAGAT CAGACTGTAT CGAGGGGATT CCTACACTGT ACGTTGGAGA CTTAGCCAAC 
ATTTCTTCCA ATCCCAAAGA AAGCCATCTG CAGAGGGCAA TTCAGAAACT GAAACTTTCT 
GTACAGAATG TCCAAGATTT TTGGAATTCA TCACAAACCG CACTGATGAA AACCTTGCAG 
ATAAATAGCA CAGACACAAC GGAGTGCAGT AAGAACCCAA CGACACCAAC ATGGAAGTCA 
GATGTTTCTG CTAGAAGTGG TTACCTAACC CCCTTGCACC ATAGTGGTAA AAGGTTATTA 
AATTCTGTCC ATACATCAGA TCCTCAGACA GAAAATCCTG GATGTAGTAA AGAAATACAA 
CTAAAAACCT GCAAAAAGAG GAAAGCTCTG GACTTTCTCA ACCGCATTCC TTCACCTCCA 
CCTGTAACAC CAGTACGGCC CTTTGTATCC CCATCTTTGC AGAAGGCATT CCGACCCCCA 
CGGAGCTGTA GCGTCCAGAA GTTGGGCCCG GAAACCAAGG GTAATACTGA GAATGTCCAG 
GGAACAACCC CAGAATGCAC TAAAGACCTA GCAAAATTAG AGGGTGAATT TGTGGCTGAT 
GAAGAACTCG CCATGATCAA TACCCAAGCT TTGCTTTTAG GTCTGGAGGA AGAAAAAAAG 
AAAACCGAGC AGAAAACCAG CAGAACAGCA GGTAAAATGA CTGCCCATGA GTCTCCAATA 
GAAAATGCTT CCCCCGTTCC TGCCCAGGAA CAACAAACTG AGGAAGCATT GAACATTCCA 
GTCGGTAACT CAGAGAAATC TTACCTGTGC CTAAGAAAAA GGAAACGGAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031753096.1
CDS	281..9994
Translation	MAAPQLGKSVFYDLFSTHCSHSDLGPISLNWFEELTAEALPYKSRTCEDHECSLDDLHENNIKTPKLKSSIYSQLDSTPVIFKERLLSPLFASSLTELDKRQNATDNGNNVRLEKSNCTQMQHQASEVFSPSSRCLNESPAVIKEIFKTPLRNKYLHKTPQCDWKPDICSSLFCTPKLMKNQTGCIKESLGAEVDPEMSWSSSLATPPSPTVIIAHANDQPSGNKHAAIVQSLFPNCENMEANNLPPPETGTQNEDRQICGMGSVIQKIPERSSSIDSIIRLEKPGSCSASKTVPEASAISSRAAWKKTVANTVKDEEVSRTVENALEGMEDVLSIFFASEKTPGLRKLKNSTQARRKVESIKSQKCQVSLFKAEEGESILVPLDEHHKCDHEMLMKPEQDYKMNAVNQKIAKETTPYEWSQLNICELDITQSQDSTLHAANLCCEDITVNKSDTCNITEAEKQEEVNEPSTDNVLSNKVGKESTLNVNCINADLNNMASSSNNSVHSLNLSQCEKMDSSEISNSEVGCITKLTTHPAKITTGELSSVDDCAKKPQERVTISTSFSTLKKQSKFMYSVNTVLTGHIASNTSIATRRSLNSHLSSDEPKPHIKENQNEPESNAKYYSNGLQKPVFLEKENDNKGISLCKTISDGQNTSEGARAPDYFEERVPETKDRCKATLSIREKVVATANCFARKQLETDYPEDASAQHEALQFHAKQYVSCSMLNSNVSNDMQIKPQVLTQACDFRSSAENLVEKTKNQLRRNEDQSFSSTGIGKKPASGEKLVGDCNEGSFLHVKQEISAAQALVGNGYETRDTPDVTSDHTRSVISNHGKSLWEEETALTELPVPEILSKTFKGFKTASNKKIHISDKNIVKGCDLFKEIEFGIGDAKPCNEENKEPLKKTPFDVPGYETNLKGFKTASNKEINVSENKFAKGRLLFKDIEEESGQTTAIMAKDNELFIKPASSNKPDIFKSTCIKGMNPSENLDSMPPKKGDVQQSAGLKHSDQIDVFGFDDPPAKGNVQTLGYFNMSASETDFKGFKTASNKDIIISESTLAKGKLIFEDIEDTRYSETGRTIDCKAKGGALSTSNLVMHNTMCKNEPSTSENTNDKAPKRAKVQTPMAENSHLKEPSSDKNAMDAANHQMTLKASAYSPKAASSALPRYKKKPISTTKTSFQLNEHLTESQQAEISELSSILENADSQFDFTQFRKVPSVTEKQNSTEGGSESQNLNNSDVWKDVDFNDSFAAGRDHSEGMEAIPSSPGIKELPSAGECCSKETHDSMPDFVTLAPKQGFLVKQNERLFAGFNLASGKQVNIDNDVLKKAAELFNDIDNDKELLSHAKEESRKSNIKHSSKLINNENCGKTEHTSEHLVCQSNVSLPFIKTLGKNATILVSGNEKSQNQELQLDCLKCESTDVKHTPQKETINDNSKCNESSLSELSMKGFQTASGRNIMMSESSIQKARNIFAEEHEDSFTLRCNIQNTIQIPQPVNEPTQFPYVNLGPKPTTTSGWQEKNILRRSTEKGFMPGFCTAGGKKVSVSDDSLAKAHKLFQEECTFSKEGKLDEVKQNKLMNSEPLSLLTCESVLKQSDGFIEDISTSRNALEIRPELYPEGMCSNRASNGSGNNSEFTAGEGISININQSSLLTTGNVLKNLPSESSGHDVYSVTEHLSTVVKVKRYNDSGHFVNQNLAECNDNHVLSTQKNTANISNRNEDCTSLAPLSFSTASGKSVTVSHDSLQKARLMLSEAANDVTVDTSKQEAAYITPAIRKTEAEKEQNTVDDSDRVNANTFSFSTASGKKVNISGNSLKQVRAVCLSSDPKETSAALFNVEKSVFNEDVKDVSLLQPNVTMPKAVSFSTASGKTVQLSDESLKKARVIFSEIDTCPLMQQQTNESTVEEIVIGGGMTKSKQMPLTTEKVETTRKNNGTFGFNTASGKQVSVSESALQKVKDIFQEFDDPDNYEQNKSLVRLPVSSKIKESTPGTKRLVQTAGSSYKNDNLQCKAGNLRTFQDKQAGKKSLTYSEAAISPIITTVPGNQLTPIQLVKVSTNTSALANKTKPDLYVATAQNTPQNDFEIEAAESARAFLEDDDLTDMGSAAHETYLYLNQTSNTRTGKRSRTEGPTPGEPPIKRRLLPEFDCTTDTRQQTGILKPLISTPDMMRDRRRFLYTLPLKPSTCNPESALRQVTCIPTQAHLHSKVKIFHQSLPIKSPDVASDSTSKSYSPTAAKETINCSSASKIPAKKFVPPFKKTVATLADNQSNSVQNGSSDGLIESIVYPKEDKVETICSSKDQFDDSDILQMTSNLRCSKDLQEMRIRKKLRQKIKPHPGSLYRLKMSHVKRISLQSAVAERCPTLYSREQLYRYGIVKNHIGVSSENALSFQFHCSNYFTKELLLSGNGVQLADGGWLIPTEQGNAGKEEIYRAFCDTPGVDPKLISAEWVHNHYRWIVWKLAAMEVRFPKTFACRCLTPERVLLQLKYRYDVEIDKSQRSAIKKIMERDDSPAKTLVLCIAKIISQGTRLPNACSNKTEPADSKESSAVIEVTDSWYGIKVLLDPCLTALLHKGRLFIGQKLIVHGAELIGSDDACSPLEAPESLMLKIAANSTRPVRWHTKLGYFKDPRPFCLHLSSLLSEGGVVGCVDVVIQRIYPMQWMEKMANGLYVFRNDRAEEREAEKHSANQQKKLEMLFSKIQAEFEQREVTCNRRKGLRRRSLNAQQMQTLQDGAEIYEAIQNESDPGYLESYLSAEQLKALNHHRQLLNDKKQALIQAEFRKAIECSEQDANGCTRRDVTPVWKLRIADYRNYETDAAYILNIWRPLPDVLSLLKEGCRYKMYHLAASTSKGKSLAADLQLTATKKTRFQQLQPSESILEQIYSPREVTDFSRFQEPLFSAPYAEVDLVGLIISIYKKTGAAPVVYISDESHNIVALKFWTDLGQLGLEEITKPRTYISASNLRWRSDCIEGIPTLYVGDLANISSNPKESHLQRAIQKLKLSVQNVQDFWNSSQTALMKTLQINSTDTTECSKNPTTPTWKSDVSARSGYLTPLHHSGKRLLNSVHTSDPQTENPGCSKEIQLKTCKKRKALDFLNRIPSPPPVTPVRPFVSPSLQKAFRPPRSCSVQKLGPETKGNTENVQGTTPECTKDLAKLEGEFVADEELAMINTQALLLGLEEEKKKTEQKTSRTAGKMTAHESPIENASPVPAQEQQTEEALNIPVGNSEKSYLCLRKRKRK

Target ORF information:

RefSeq Version	XM_031897236.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis BRCA2, DNA repair associated (brca2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031897236.1

ORF Insert Sequence:

ATGGCTGCAC CGCAACTTGG AAAATCTGTC TTCTATGATC TGTTTAGCAC GCATTGCTCT 
CACTCAGATT TAGGGCCTAT CAGTCTAAAC TGGTTTGAAG AGCTCACTGC TGAAGCCTTG 
CCTTACAAGT CAAGAACATG TGAAGATCAT GAATGCAGTT TGGATGATCT CCATGAAAAC 
AACATCAAAA CTCCTAAACT GAAGTCGTCA ATTTATAGCC AATTAGATTC CACTCCAGTC 
ATCTTTAAGG AGCGTTTACT ATCACCTCTT TTTGCTTCTT CTTTGACAGA GCTGGATAAA 
AGGCAGAATG CAACAGATAA TGGAAACAAT GTTAGACTTG AGAAAAGTAA CTGTACCCAA 
ATGCAGCATC AAGCAAGTGA AGTTTTCAGT CCCTCAAGTC GATGCCTCAA CGAAAGTCCT 
GCTGTTATCA AAGAAATATT CAAGACACCA TTACGAAATA AGTACCTACA TAAAACACCA 
CAGTGTGATT GGAAACCTGA CATTTGCAGC AGTCTGTTTT GTACTCCAAA GCTTATGAAG 
AACCAAACTG GATGTATTAA AGAAAGTCTA GGTGCAGAGG TGGATCCAGA GATGTCATGG 
TCTAGTTCAC TAGCTACACC TCCTTCCCCA ACTGTGATAA TTGCACATGC TAATGATCAG 
CCCAGTGGAA ATAAACATGC TGCAATTGTA CAGAGCCTTT TCCCAAATTG TGAGAATATG 
GAAGCGAATA ATTTGCCACC ACCTGAGACG GGCACACAAA ATGAAGACCG TCAAATATGT 
GGTATGGGAT CTGTTATCCA GAAAATTCCG GAAAGGTCAT CTTCCATAGA CTCCATTATA 
AGACTAGAAA AGCCAGGTTC ATGCAGTGCG AGCAAAACTG TACCAGAAGC GTCTGCCATT 
TCCTCAAGGG CAGCCTGGAA GAAAACTGTT GCAAATACAG TGAAGGACGA GGAGGTTTCC 
AGAACTGTGG AAAATGCTCT GGAAGGAATG GAAGATGTCC TCTCAATCTT TTTTGCAAGT 
GAAAAGACTC CTGGTCTCAG AAAGCTGAAA AATTCTACCC AAGCTAGGAG AAAAGTGGAA 
TCTATTAAGT CTCAGAAGTG CCAAGTTTCA TTATTTAAAG CAGAAGAAGG AGAAAGCATT 
TTGGTCCCTT TAGATGAGCA TCATAAATGT GATCATGAGA TGCTTATGAA ACCTGAACAA 
GACTACAAAA TGAATGCTGT TAACCAAAAA ATAGCCAAAG AAACTACTCC ATATGAATGG 
TCACAGCTAA ATATATGTGA GTTGGATATA ACTCAATCAC AGGATAGCAC TTTGCATGCA 
GCAAATCTAT GTTGTGAAGA TATCACTGTC AACAAAAGTG ATACATGTAA TATTACTGAA 
GCCGAAAAGC AGGAGGAAGT CAATGAGCCA AGCACCGACA ATGTACTGTC TAATAAAGTT 
GGCAAAGAAA GCACTTTAAA TGTGAATTGT ATTAATGCTG ATTTGAATAA CATGGCTTCA 
TCCTCAAATA ACTCTGTTCA CTCACTGAAT CTTTCTCAAT GTGAAAAAAT GGATTCTTCT 
GAAATTTCTA ACAGTGAAGT TGGTTGTATA ACAAAACTAA CAACCCACCC AGCAAAAATA 
ACAACTGGTG AATTAAGTTC AGTAGATGAC TGTGCTAAGA AACCCCAGGA AAGAGTAACT 
ATTAGTACTT CATTTTCAAC TCTGAAAAAG CAATCGAAGT TTATGTACAG TGTAAACACT 
GTGTTAACTG GTCATATTGC AAGCAATACC AGCATAGCCA CTAGGAGATC ATTGAACAGT 
CATTTAAGTT CTGATGAACC AAAACCTCAC ATTAAGGAAA ATCAAAATGA ACCAGAATCA 
AATGCTAAAT ATTATTCCAA CGGCCTGCAG AAACCAGTAT TCCTGGAAAA GGAGAATGAT 
AATAAGGGAA TTTCTTTATG TAAAACTATC TCAGACGGTC AAAACACTTC AGAAGGTGCC 
AGGGCTCCAG ACTATTTTGA GGAACGTGTT CCTGAAACAA AAGATAGATG CAAAGCTACA 
CTAAGCATCA GGGAAAAGGT TGTGGCTACA GCAAATTGTT TTGCAAGAAA ACAGCTTGAA 
ACAGATTATC CAGAGGATGC CTCAGCACAA CATGAGGCAC TTCAGTTCCA TGCTAAGCAA 
TATGTGTCAT GCTCAATGCT AAATTCTAAT GTGTCCAATG ACATGCAAAT AAAGCCTCAA 
GTGTTAACTC AGGCATGCGA TTTTAGGAGC AGCGCAGAAA ATCTTGTTGA AAAAACAAAA 
AATCAATTAA GGCGGAATGA AGATCAATCA TTTTCATCAA CTGGCATAGG GAAAAAGCCA 
GCTAGTGGTG AAAAATTAGT AGGTGACTGT AATGAGGGCT CATTCCTTCA TGTGAAGCAA 
GAAATAAGTG CTGCTCAGGC ATTGGTAGGT AATGGTTACG AAACCAGAGA CACTCCTGAT 
GTCACTAGTG ATCATACAAG GAGTGTTATT TCCAACCATG GAAAGTCTCT GTGGGAGGAG 
GAGACAGCAC TGACTGAATT ACCAGTCCCA GAAATTCTGA GTAAAACTTT CAAGGGCTTT 
AAAACGGCAT CTAATAAAAA AATACATATT TCAGATAAAA ATATTGTAAA AGGTTGTGAC 
TTGTTCAAAG AAATTGAGTT TGGCATTGGT GACGCTAAGC CCTGCAATGA AGAAAACAAA 
GAGCCCTTAA AGAAAACTCC CTTTGATGTT CCTGGGTATG AAACAAATTT AAAAGGATTC 
AAAACTGCAT CCAATAAAGA AATAAATGTA TCAGAAAACA AATTTGCTAA AGGTAGACTG 
CTGTTTAAAG ACATCGAAGA GGAAAGCGGT CAAACTACAG CTATAATGGC AAAAGATAAT 
GAGCTGTTCA TTAAGCCTGC ATCCAGCAAT AAGCCTGACA TATTTAAGAG TACATGTATA 
AAAGGAATGA ATCCATCTGA GAACCTAGAC AGTATGCCCC CCAAAAAAGG AGACGTGCAG 
CAGAGTGCAG GTTTAAAACA TTCAGATCAA ATTGACGTTT TTGGTTTCGA TGATCCTCCT 
GCAAAAGGAA ATGTTCAAAC ATTAGGCTAT TTTAATATGT CTGCTTCTGA AACAGACTTC 
AAAGGATTCA AAACCGCTTC CAATAAAGAC ATAATTATTT CAGAAAGCAC ACTTGCTAAA 
GGTAAACTTA TTTTTGAAGA TATTGAAGAT ACAAGATACA GTGAAACAGG AAGGACCATA 
GATTGTAAAG CAAAAGGTGG TGCGTTGTCC ACCAGTAACC TTGTAATGCA TAACACCATG 
TGTAAAAATG AACCAAGTAC ATCTGAGAAC ACAAATGATA AAGCACCAAA AAGAGCAAAA 
GTACAAACAC CTATGGCAGA AAATAGTCAT TTAAAAGAAC CTTCTTCAGA CAAAAATGCA 
ATGGATGCTG CAAATCATCA AATGACACTG AAAGCCTCCG CATACAGCCC AAAGGCTGCC 
TCTTCTGCAC TGCCCAGGTA CAAAAAGAAA CCAATCTCTA CCACTAAAAC CTCTTTCCAG 
TTGAATGAAC ATTTGACTGA AAGCCAGCAA GCTGAAATCT CTGAACTTTC CTCTATTCTA 
GAAAATGCAG ACAGTCAGTT TGATTTTACT CAGTTTAGGA AAGTGCCTTC TGTGACTGAG 
AAACAAAATT CAACTGAAGG TGGGAGCGAG TCCCAGAACC TTAACAACTC AGATGTCTGG 
AAGGACGTAG ATTTTAATGA TAGTTTTGCA GCAGGAAGGG ATCATTCTGA GGGAATGGAG 
GCAATTCCCT CCAGTCCTGG AATAAAAGAA TTGCCTTCTG CAGGAGAATG TTGCAGTAAA 
GAAACACATG ATTCTATGCC AGATTTTGTG ACCTTGGCAC CTAAACAAGG TTTTCTGGTT 
AAACAAAATG AAAGACTCTT TGCAGGGTTT AATCTTGCTA GTGGCAAGCA AGTTAATATA 
GACAATGATG TACTAAAAAA AGCTGCTGAA CTGTTCAATG ATATAGATAA TGATAAAGAA 
TTGCTCAGCC ACGCCAAAGA AGAAAGCAGA AAAAGCAATA TAAAACACTC TTCTAAATTA 
ATAAATAATG AAAATTGTGG AAAGACAGAA CACACATCTG AGCATTTGGT ATGTCAAAGT 
AATGTTTCAT TACCATTCAT AAAAACATTG GGCAAGAATG CTACAATACT AGTGAGTGGT 
AATGAAAAAA GCCAGAATCA AGAATTACAG CTGGATTGTT TAAAATGTGA AAGTACTGAT 
GTAAAGCATA CACCACAAAA GGAAACAATC AATGACAACT CAAAGTGTAA TGAATCCTCT 
CTTTCTGAGC TTTCCATGAA AGGTTTTCAG ACTGCCAGTG GGAGAAACAT TATGATGTCC 
GAGTCTTCAA TCCAGAAAGC CAGAAACATA TTTGCAGAGG AACATGAAGA CAGTTTTACA 
TTGCGCTGTA ATATTCAAAA TACAATACAA ATACCACAGC CTGTAAATGA ACCGACCCAA 
TTCCCATATG TTAATTTGGG GCCAAAACCC ACAACGACAT CAGGATGGCA AGAAAAAAAT 
ATTCTGAGAA GAAGTACAGA AAAAGGTTTC ATGCCGGGTT TTTGCACTGC CGGTGGTAAA 
AAGGTTTCTG TTTCCGACGA CTCTCTAGCT AAAGCACATA AACTTTTCCA GGAGGAATGT 
ACCTTTAGCA AGGAAGGGAA ATTGGATGAG GTGAAACAAA ATAAATTAAT GAACTCTGAG 
CCATTGTCTC TTCTCACTTG TGAGTCAGTC CTGAAACAGA GTGACGGATT CATTGAAGAT 
ATTTCAACTT CTAGAAATGC TTTGGAAATC AGACCAGAGC TTTATCCGGA GGGCATGTGT 
AGTAATAGGG CATCTAATGG AAGTGGAAAC AATAGTGAAT TTACAGCTGG GGAAGGCATA 
TCAATAAATA TCAACCAATC ATCGCTGCTT ACAACAGGCA ACGTTTTAAA AAATTTGCCG 
TCTGAAAGTA GTGGCCATGA TGTGTATTCA GTAACTGAGC ATCTAAGCAC TGTGGTTAAA 
GTCAAAAGAT ACAATGATTC AGGTCATTTT GTAAATCAAA ATTTAGCTGA ATGTAATGAT 
AACCATGTCC TTAGCACTCA AAAAAATACT GCAAATATAA GCAACAGAAA CGAGGACTGT 
ACATCCCTGG CACCTCTCTC TTTCAGCACA GCCAGTGGGA AATCCGTCAC AGTGTCTCAT 
GATTCTCTTC AAAAAGCAAG ACTGATGCTT TCTGAAGCAG CTAATGATGT TACAGTAGAT 
ACTAGTAAGC AAGAGGCGGC TTATATTACG CCAGCCATTC GAAAAACAGA AGCAGAAAAA 
GAGCAGAACA CTGTTGATGA TTCTGATAGA GTTAATGCAA ATACATTTTC ATTTTCTACT 
GCAAGCGGTA AAAAGGTCAA CATAAGCGGA AATTCTTTAA AGCAGGTCAG AGCTGTCTGC 
CTATCCTCTG ACCCAAAAGA AACATCAGCG GCTCTGTTTA ATGTTGAAAA AAGTGTTTTT 
AATGAAGATG TTAAGGATGT ATCTTTGCTT CAGCCCAATG TTACCATGCC GAAGGCTGTG 
TCGTTTAGTA CTGCAAGTGG CAAGACGGTG CAGTTGTCAG ACGAGTCTTT GAAGAAAGCT 
AGGGTGATTT TTTCTGAAAT AGATACTTGT CCGTTGATGC AACAGCAGAC TAATGAAAGC 
ACTGTTGAAG AGATAGTAAT AGGAGGAGGA ATGACAAAAT CAAAACAGAT GCCATTAACA 
ACAGAAAAAG TCGAAACAAC TAGAAAAAAT AATGGTACTT TTGGCTTTAA TACCGCAAGC 
GGAAAGCAGG TCTCTGTGTC TGAAAGTGCG CTGCAGAAAG TCAAAGATAT CTTTCAAGAA 
TTTGATGATC CCGACAATTA TGAACAAAAT AAATCACTCG TAAGACTGCC TGTTTCATCA 
AAAATAAAAG AATCAACTCC AGGAACAAAA AGACTGGTGC AAACTGCAGG GTCCTCTTAT 
AAAAATGATA ATTTGCAATG TAAAGCAGGG AATCTTCGTA CTTTTCAGGA TAAGCAAGCT 
GGCAAGAAGT CACTTACATA TTCAGAAGCA GCCATAAGCC CCATTATAAC AACTGTCCCT 
GGAAATCAAT TAACGCCCAT CCAGTTGGTA AAAGTCTCAA CCAACACATC AGCATTGGCT 
AATAAAACCA AACCCGATTT ATATGTCGCA ACAGCCCAGA ACACACCACA AAATGATTTT 
GAAATAGAAG CAGCAGAAAG TGCTAGAGCT TTCTTGGAAG ATGATGACCT GACAGATATG 
GGATCAGCTG CCCATGAAAC ATACTTGTAT CTCAACCAGA CCTCAAATAC AAGAACTGGG 
AAGAGATCAA GAACAGAAGG GCCGACACCT GGAGAGCCAC CAATCAAAAG ACGGCTTTTA 
CCAGAATTTG ATTGCACAAC AGACACTCGG CAACAAACGG GGATTTTAAA GCCTTTGATC 
AGCACTCCAG ACATGATGCG AGACAGGCGA AGGTTTTTGT ACACCCTTCC CTTGAAGCCA 
TCAACTTGTA ATCCTGAAAG TGCTTTACGT CAAGTCACGT GCATTCCTAC ACAAGCCCAT 
CTCCATTCTA AAGTCAAAAT CTTCCACCAG TCTCTACCCA TCAAGTCACC AGATGTTGCA 
TCTGATTCAA CTTCTAAATC GTATAGCCCC ACTGCTGCAA AAGAGACCAT AAACTGCTCA 
TCTGCATCTA AAATACCTGC AAAGAAATTT GTGCCTCCTT TTAAAAAGAC TGTGGCGACC 
TTGGCAGACA ACCAGTCAAA CAGTGTACAA AATGGCAGTT CGGATGGGCT TATCGAGAGC 
ATTGTTTATC CAAAAGAAGA CAAAGTTGAA ACCATATGTT CTTCAAAAGA TCAATTTGAT 
GACTCAGATA TTTTACAAAT GACTTCAAAT CTTCGATGTA GCAAAGACCT GCAGGAAATG 
AGAATTCGGA AAAAGCTAAG GCAAAAGATT AAACCGCATC CAGGCTCACT GTATCGTCTG 
AAAATGTCAC ATGTGAAGAG AATTTCACTT CAATCTGCAG TGGCAGAAAG GTGTCCCACT 
CTGTATTCAA GGGAACAGTT ATACAGATAT GGCATTGTGA AGAACCATAT TGGAGTCAGC 
AGCGAGAACG CATTGTCCTT CCAGTTCCAC TGTTCAAATT ATTTCACAAA AGAACTTCTT 
CTTTCTGGAA ATGGTGTGCA GTTGGCAGAT GGAGGTTGGC TTATTCCCAC TGAACAAGGC 
AACGCAGGCA AAGAAGAGAT ATACAGAGCG TTTTGTGACA CTCCTGGTGT GGATCCAAAA 
TTAATTTCAG CTGAATGGGT TCATAACCAC TACCGCTGGA TTGTATGGAA ACTTGCTGCA 
ATGGAAGTCA GGTTCCCAAA GACATTTGCT TGCAGATGCC TTACACCAGA ACGTGTGCTT 
TTACAGTTAA AGTACAGATA TGATGTGGAA ATTGATAAGA GTCAGAGATC CGCCATCAAA 
AAGATCATGG AGAGAGATGA CTCCCCAGCT AAAACGCTGG TACTGTGCAT TGCCAAGATC 
ATTTCCCAGG GCACTCGCCT GCCCAATGCA TGTAGTAATA AAACCGAACC CGCTGACTCT 
AAGGAAAGTT CTGCAGTAAT AGAGGTGACA GATAGTTGGT ATGGTATCAA GGTTCTGCTT 
GATCCGTGTC TCACTGCTCT CTTACACAAA GGAAGGCTTT TTATTGGTCA GAAGCTAATT 
GTTCATGGAG CGGAACTTAT TGGCTCAGAT GATGCCTGCT CTCCCCTTGA AGCGCCAGAA 
TCTCTTATGT TGAAGATTGC AGCTAACAGC ACTCGGCCTG TTCGATGGCA TACCAAACTT 
GGATATTTCA AGGATCCCAG ACCATTCTGT CTCCATTTAT CATCACTGTT AAGTGAAGGA 
GGTGTGGTGG GGTGTGTCGA TGTAGTAATA CAAAGAATCT ATCCCATGCA GTGGATGGAA 
AAAATGGCAA ATGGACTGTA TGTGTTTCGC AATGATCGAG CAGAGGAAAG AGAAGCAGAA 
AAGCATTCAG CAAATCAACA AAAAAAGCTT GAAATGCTCT TTTCAAAAAT ACAAGCTGAA 
TTTGAACAGC GGGAAGTAAC ATGCAACAGA AGAAAGGGAC TCAGGCGGCG GTCATTAAAT 
GCACAACAAA TGCAGACCCT TCAGGATGGG GCTGAAATCT ATGAAGCCAT TCAGAACGAA 
TCTGACCCTG GGTATCTGGA GTCGTACTTA AGTGCTGAGC AGCTGAAAGC CCTCAATCAC 
CACAGGCAAC TTTTAAACGA TAAGAAGCAA GCACTGATCC AAGCTGAATT CAGAAAAGCC 
ATTGAATGCA GTGAGCAGGA TGCAAATGGC TGTACAAGGA GGGATGTCAC TCCTGTATGG 
AAATTACGCA TTGCAGATTA CAGAAATTAT GAAACAGATG CAGCATATAT ACTGAACATT 
TGGCGTCCGT TACCAGATGT GCTTTCTCTT CTGAAAGAAG GTTGTCGCTA TAAAATGTAT 
CATTTGGCAG CATCCACATC AAAAGGAAAG TCATTAGCAG CTGATTTGCA GTTAACAGCT 
ACAAAGAAAA CTCGCTTCCA GCAACTTCAG CCATCAGAGA GCATTTTGGA GCAGATTTAC 
AGCCCGAGAG AAGTGACGGA CTTCAGCAGG TTCCAGGAGC CTTTGTTTAG CGCCCCATAT 
GCAGAGGTTG ATCTGGTGGG ATTAATCATC TCCATATACA AAAAAACAGG TGCAGCTCCA 
GTGGTTTATA TATCAGATGA ATCTCACAAT ATTGTGGCGC TAAAATTCTG GACAGATCTA 
GGGCAGCTTG GGCTTGAGGA GATAACCAAG CCACGCACAT ATATCTCTGC CAGTAATCTT 
CGCTGGAGAT CAGACTGTAT CGAGGGGATT CCTACACTGT ACGTTGGAGA CTTAGCCAAC 
ATTTCTTCCA ATCCCAAAGA AAGCCATCTG CAGAGGGCAA TTCAGAAACT GAAACTTTCT 
GTACAGAATG TCCAAGATTT TTGGAATTCA TCACAAACCG CACTGATGAA AACCTTGCAG 
ATAAATAGCA CAGACACAAC GGAGTGCAGT AAGAACCCAA CGACACCAAC ATGGAAGTCA 
GATGTTTCTG CTAGAAGTGG TTACCTAACC CCCTTGCACC ATAGTGGTAA AAGGTTATTA 
AATTCTGTCC ATACATCAGA TCCTCAGACA GAAAATCCTG GATGTAGTAA AGAAATACAA 
CTAAAAACCT GCAAAAAGAG GAAAGCTCTG GACTTTCTCA ACCGCATTCC TTCACCTCCA 
CCTGTAACAC CAGTACGGCC CTTTGTATCC CCATCTTTGC AGAAGGCATT CCGACCCCCA 
CGGAGCTGTA GCGTCCAGAA GTTGGGCCCG GAAACCAAGG GTAATACTGA GAATGTCCAG 
GGAACAACCC CAGAATGCAC TAAAGACCTA GCAAAATTAG AGGGTGAATT TGTGGCTGAT 
GAAGAACTCG CCATGATCAA TACCCAAGCT TTGCTTTTAG GTCTGGAGGA AGAAAAAAAG 
AAAACCGAGC AGAAAACCAG CAGAACAGCA GGTAAAATGA CTGCCCATGA GTCTCCAATA 
GAAAATGCTT CCCCCGTTCC TGCCCAGGAA CAACAAACTG AGGAAGCATT GAACATTCCA 
GTCGGTAACT CAGAGAAATC TTACCTGTGC CTAAGAAAAA GGAAACGGAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

brca2 ( XM_018091730.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947219.2 Xenopus tropicalis BRCA2, DNA repair associated (brca2), transcript variant X4, mRNA.

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CloneID	OXa52807
Clone ID Related Accession (Same CDS sequence)	XM_018091730.2
Accession Version	XM_018091730.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	breast cancer type 2 susceptibility protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018091730.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAC CGCAACTTGG AAAATCTGTC TTCTATGATC TGTTTAGCAC GCATTGCTCT 
CACTCAGATT TAGGGCCTAT CAGTCTAAAC TGGTTTGAAG AGCTCACTGC TGAAGCCTTG 
CCTTACAAGT CAAGAACATG TGAAGATCAT GAATGCAGTT TGGATGATCT CCATGAAAAC 
AACATCAAAA CTCCTAAACT GAAGTCGTCA ATTTATAGCC AATTAGATTC CACTCCAGTC 
ATCTTTAAGG AGCGTTTACT ATCACCTCTT TTTGCTTCTT CTTTGACAGA GCTGGATAAA 
AGGCAGAATG CAACAGATAA TGGAAACAAT GTTAGACTTG AGAAAAGTAA CTGTACCCAA 
ATGCAGCATC AAGCAAGTGA AGTTTTCAGT CCCTCAAGTC GATGCCTCAA CGAAAGTCCT 
GCTGTTATCA AAGAAATATT CAAGACACCA TTACGAAATA AGTACCTACA TAAAACACCA 
CAGTGTGATT GGAAACCTGA CATTTGCAGC AGTCTGTTTT GTACTCCAAA GCTTATGAAG 
AACCAAACTG GATGTATTAA AGAAAGTCTA GGTGCAGAGG TGGATCCAGA GATGTCATGG 
TCTAGTTCAC TAGCTACACC TCCTTCCCCA ACTGTGATAA TTGCACATGC TAATGATCAG 
CCCAGTGGAA ATAAACATGC TGCAATTGTA CAGAGCCTTT TCCCAAATTG TGAGAATATG 
GAAGCGAATA ATTTGCCACC ACCTGAGACG GGCACACAAA ATGAAGACCG TCAAATATGT 
GCAGGACTAG AAAAGCCAGG TTCATGCAGT GCGAGCAAAA CTGTACCAGA AGCGTCTGCC 
ATTTCCTCAA GGGCAGCCTG GAAGAAAACT GTTGCAAATA CAGTGAAGGA CGAGGAGGTT 
TCCAGAACTG TGGAAAATGC TCTGGAAGGA ATGGAAGATG TCCTCTCAAT CTTTTTTGCA 
AGTGAAAAGA CTCCTGGTCT CAGAAAGCTG AAAAATTCTA CCCAAGCTAG GAGAAAAGTG 
GAATCTATTA AGTCTCAGAA GTGCCAAGTT TCATTATTTA AAGCAGAAGA AGGAGAAAGC 
ATTTTGGTCC CTTTAGATGA GCATCATAAA TGTGATCATG AGATGCTTAT GAAACCTGAA 
CAAGACTACA AAATGAATGC TGTTAACCAA AAAATAGCCA AAGAAACTAC TCCATATGAA 
TGGTCACAGC TAAATATATG TGAGTTGGAT ATAACTCAAT CACAGGATAG CACTTTGCAT 
GCAGCAAATC TATGTTGTGA AGATATCACT GTCAACAAAA GTGATACATG TAATATTACT 
GAAGCCGAAA AGCAGGAGGA AGTCAATGAG CCAAGCACCG ACAATGTACT GTCTAATAAA 
GTTGGCAAAG AAAGCACTTT AAATGTGAAT TGTATTAATG CTGATTTGAA TAACATGGCT 
TCATCCTCAA ATAACTCTGT TCACTCACTG AATCTTTCTC AATGTGAAAA AATGGATTCT 
TCTGAAATTT CTAACAGTGA AGTTGGTTGT ATAACAAAAC TAACAACCCA CCCAGCAAAA 
ATAACAACTG GTGAATTAAG TTCAGTAGAT GACTGTGCTA AGAAACCCCA GGAAAGAGTA 
ACTATTAGTA CTTCATTTTC AACTCTGAAA AAGCAATCGA AGTTTATGTA CAGTGTAAAC 
ACTGTGTTAA CTGGTCATAT TGCAAGCAAT ACCAGCATAG CCACTAGGAG ATCATTGAAC 
AGTCATTTAA GTTCTGATGA ACCAAAACCT CACATTAAGG AAAATCAAAA TGAACCAGAA 
TCAAATGCTA AATATTATTC CAACGGCCTG CAGAAACCAG TATTCCTGGA AAAGGAGAAT 
GATAATAAGG GAATTTCTTT ATGTAAAACT ATCTCAGACG GTCAAAACAC TTCAGAAGGT 
GCCAGGGCTC CAGACTATTT TGAGGAACGT GTTCCTGAAA CAAAAGATAG ATGCAAAGCT 
ACACTAAGCA TCAGGGAAAA GGTTGTGGCT ACAGCAAATT GTTTTGCAAG AAAACAGCTT 
GAAACAGATT ATCCAGAGGA TGCCTCAGCA CAACATGAGG CACTTCAGTT CCATGCTAAG 
CAATATGTGT CATGCTCAAT GCTAAATTCT AATGTGTCCA ATGACATGCA AATAAAGCCT 
CAAGTGTTAA CTCAGGCATG CGATTTTAGG AGCAGCGCAG AAAATCTTGT TGAAAAAACA 
AAAAATCAAT TAAGGCGGAA TGAAGATCAA TCATTTTCAT CAACTGGCAT AGGGAAAAAG 
CCAGCTAGTG GTGAAAAATT AGTAGGTGAC TGTAATGAGG GCTCATTCCT TCATGTGAAG 
CAAGAAATAA GTGCTGCTCA GGCATTGGTA GGTAATGGTT ACGAAACCAG AGACACTCCT 
GATGTCACTA GTGATCATAC AAGGAGTGTT ATTTCCAACC ATGGAAAGTC TCTGTGGGAG 
GAGGAGACAG CACTGACTGA ATTACCAGTC CCAGAAATTC TGAGTAAAAC TTTCAAGGGC 
TTTAAAACGG CATCTAATAA AAAAATACAT ATTTCAGATA AAAATATTGT AAAAGGTTGT 
GACTTGTTCA AAGAAATTGA GTTTGGCATT GGTGACGCTA AGCCCTGCAA TGAAGAAAAC 
AAAGAGCCCT TAAAGAAAAC TCCCTTTGAT GTTCCTGGGT ATGAAACAAA TTTAAAAGGA 
TTCAAAACTG CATCCAATAA AGAAATAAAT GTATCAGAAA ACAAATTTGC TAAAGGTAGA 
CTGCTGTTTA AAGACATCGA AGAGGAAAGC GGTCAAACTA CAGCTATAAT GGCAAAAGAT 
AATGAGCTGT TCATTAAGCC TGCATCCAGC AATAAGCCTG ACATATTTAA GAGTACATGT 
ATAAAAGGAA TGAATCCATC TGAGAACCTA GACAGTATGC CCCCCAAAAA AGGAGACGTG 
CAGCAGAGTG CAGGTTTAAA ACATTCAGAT CAAATTGACG TTTTTGGTTT CGATGATCCT 
CCTGCAAAAG GAAATGTTCA AACATTAGGC TATTTTAATA TGTCTGCTTC TGAAACAGAC 
TTCAAAGGAT TCAAAACCGC TTCCAATAAA GACATAATTA TTTCAGAAAG CACACTTGCT 
AAAGGTAAAC TTATTTTTGA AGATATTGAA GATACAAGAT ACAGTGAAAC AGGAAGGACC 
ATAGATTGTA AAGCAAAAGG TGGTGCGTTG TCCACCAGTA ACCTTGTAAT GCATAACACC 
ATGTGTAAAA ATGAACCAAG TACATCTGAG AACACAAATG ATAAAGCACC AAAAAGAGCA 
AAAGTACAAA CACCTATGGC AGAAAATAGT CATTTAAAAG AACCTTCTTC AGACAAAAAT 
GCAATGGATG CTGCAAATCA TCAAATGACA CTGAAAGCCT CCGCATACAG CCCAAAGGCT 
GCCTCTTCTG CACTGCCCAG GTACAAAAAG AAACCAATCT CTACCACTAA AACCTCTTTC 
CAGTTGAATG AACATTTGAC TGAAAGCCAG CAAGCTGAAA TCTCTGAACT TTCCTCTATT 
CTAGAAAATG CAGACAGTCA GTTTGATTTT ACTCAGTTTA GGAAAGTGCC TTCTGTGACT 
GAGAAACAAA ATTCAACTGA AGGTGGGAGC GAGTCCCAGA ACCTTAACAA CTCAGATGTC 
TGGAAGGACG TAGATTTTAA TGATAGTTTT GCAGCAGGAA GGGATCATTC TGAGGGAATG 
GAGGCAATTC CCTCCAGTCC TGGAATAAAA GAATTGCCTT CTGCAGGAGA ATGTTGCAGT 
AAAGAAACAC ATGATTCTAT GCCAGATTTT GTGACCTTGG CACCTAAACA AGGTTTTCTG 
GTTAAACAAA ATGAAAGACT CTTTGCAGGG TTTAATCTTG CTAGTGGCAA GCAAGTTAAT 
ATAGACAATG ATGTACTAAA AAAAGCTGCT GAACTGTTCA ATGATATAGA TAATGATAAA 
GAATTGCTCA GCCACGCCAA AGAAGAAAGC AGAAAAAGCA ATATAAAACA CTCTTCTAAA 
TTAATAAATA ATGAAAATTG TGGAAAGACA GAACACACAT CTGAGCATTT GGTATGTCAA 
AGTAATGTTT CATTACCATT CATAAAAACA TTGGGCAAGA ATGCTACAAT ACTAGTGAGT 
GGTAATGAAA AAAGCCAGAA TCAAGAATTA CAGCTGGATT GTTTAAAATG TGAAAGTACT 
GATGTAAAGC ATACACCACA AAAGGAAACA ATCAATGACA ACTCAAAGTG TAATGAATCC 
TCTCTTTCTG AGCTTTCCAT GAAAGGTTTT CAGACTGCCA GTGGGAGAAA CATTATGATG 
TCCGAGTCTT CAATCCAGAA AGCCAGAAAC ATATTTGCAG AGGAACATGA AGACAGTTTT 
ACATTGCGCT GTAATATTCA AAATACAATA CAAATACCAC AGCCTGTAAA TGAACCGACC 
CAATTCCCAT ATGTTAATTT GGGGCCAAAA CCCACAACGA CATCAGGATG GCAAGAAAAA 
AATATTCTGA GAAGAAGTAC AGAAAAAGGT TTCATGCCGG GTTTTTGCAC TGCCGGTGGT 
AAAAAGGTTT CTGTTTCCGA CGACTCTCTA GCTAAAGCAC ATAAACTTTT CCAGGAGGAA 
TGTACCTTTA GCAAGGAAGG GAAATTGGAT GAGGTGAAAC AAAATAAATT AATGAACTCT 
GAGCCATTGT CTCTTCTCAC TTGTGAGTCA GTCCTGAAAC AGAGTGACGG ATTCATTGAA 
GATATTTCAA CTTCTAGAAA TGCTTTGGAA ATCAGACCAG AGCTTTATCC GGAGGGCATG 
TGTAGTAATA GGGCATCTAA TGGAAGTGGA AACAATAGTG AATTTACAGC TGGGGAAGGC 
ATATCAATAA ATATCAACCA ATCATCGCTG CTTACAACAG GCAACGTTTT AAAAAATTTG 
CCGTCTGAAA GTAGTGGCCA TGATGTGTAT TCAGTAACTG AGCATCTAAG CACTGTGGTT 
AAAGTCAAAA GATACAATGA TTCAGGTCAT TTTGTAAATC AAAATTTAGC TGAATGTAAT 
GATAACCATG TCCTTAGCAC TCAAAAAAAT ACTGCAAATA TAAGCAACAG AAACGAGGAC 
TGTACATCCC TGGCACCTCT CTCTTTCAGC ACAGCCAGTG GGAAATCCGT CACAGTGTCT 
CATGATTCTC TTCAAAAAGC AAGACTGATG CTTTCTGAAG CAGCTAATGA TGTTACAGTA 
GATACTAGTA AGCAAGAGGC GGCTTATATT ACGCCAGCCA TTCGAAAAAC AGAAGCAGAA 
AAAGAGCAGA ACACTGTTGA TGATTCTGAT AGAGTTAATG CAAATACATT TTCATTTTCT 
ACTGCAAGCG GTAAAAAGGT CAACATAAGC GGAAATTCTT TAAAGCAGGT CAGAGCTGTC 
TGCCTATCCT CTGACCCAAA AGAAACATCA GCGGCTCTGT TTAATGTTGA AAAAAGTGTT 
TTTAATGAAG ATGTTAAGGA TGTATCTTTG CTTCAGCCCA ATGTTACCAT GCCGAAGGCT 
GTGTCGTTTA GTACTGCAAG TGGCAAGACG GTGCAGTTGT CAGACGAGTC TTTGAAGAAA 
GCTAGGGTGA TTTTTTCTGA AATAGATACT TGTCCGTTGA TGCAACAGCA GACTAATGAA 
AGCACTGTTG AAGAGATAGT AATAGGAGGA GGAATGACAA AATCAAAACA GATGCCATTA 
ACAACAGAAA AAGTCGAAAC AACTAGAAAA AATAATGGTA CTTTTGGCTT TAATACCGCA 
AGCGGAAAGC AGGTCTCTGT GTCTGAAAGT GCGCTGCAGA AAGTCAAAGA TATCTTTCAA 
GAATTTGATG ATCCCGACAA TTATGAACAA AATAAATCAC TCGTAAGACT GCCTGTTTCA 
TCAAAAATAA AAGAATCAAC TCCAGGAACA AAAAGACTGG TGCAAACTGC AGGGTCCTCT 
TATAAAAATG ATAATTTGCA ATGTAAAGCA GGGAATCTTC GTACTTTTCA GGATAAGCAA 
GCTGGCAAGA AGTCACTTAC ATATTCAGAA GCAGCCATAA GCCCCATTAT AACAACTGTC 
CCTGGAAATC AATTAACGCC CATCCAGTTG GTAAAAGTCT CAACCAACAC ATCAGCATTG 
GCTAATAAAA CCAAACCCGA TTTATATGTC GCAACAGCCC AGAACACACC ACAAAATGAT 
TTTGAAATAG AAGCAGCAGA AAGTGCTAGA GCTTTCTTGG AAGATGATGA CCTGACAGAT 
ATGGGATCAG CTGCCCATGA AACATACTTG TATCTCAACC AGACCTCAAA TACAAGAACT 
GGGAAGAGAT CAAGAACAGA AGGGCCGACA CCTGGAGAGC CACCAATCAA AAGACGGCTT 
TTACCAGAAT TTGATTGCAC AACAGACACT CGGCAACAAA CGGGGATTTT AAAGCCTTTG 
ATCAGCACTC CAGACATGAT GCGAGACAGG CGAAGGTTTT TGTACACCCT TCCCTTGAAG 
CCATCAACTT GTAATCCTGA AAGTGCTTTA CGTCAAGTCA CGTGCATTCC TACACAAGCC 
CATCTCCATT CTAAAGTCAA AATCTTCCAC CAGTCTCTAC CCATCAAGTC ACCAGATGTT 
GCATCTGATT CAACTTCTAA ATCGTATAGC CCCACTGCTG CAAAAGAGAC CATAAACTGC 
TCATCTGCAT CTAAAATACC TGCAAAGAAA TTTGTGCCTC CTTTTAAAAA GACTGTGGCG 
ACCTTGGCAG ACAACCAGTC AAACAGTGTA CAAAATGGCA GTTCGGATGG GCTTATCGAG 
AGCATTGTTT ATCCAAAAGA AGACAAAGTT GAAACCATAT GTTCTTCAAA AGATCAATTT 
GATGACTCAG ATATTTTACA AATGACTTCA AATCTTCGAT GTAGCAAAGA CCTGCAGGAA 
ATGAGAATTC GGAAAAAGCT AAGGCAAAAG ATTAAACCGC ATCCAGGCTC ACTGTATCGT 
CTGAAAATGT CACATGTGAA GAGAATTTCA CTTCAATCTG CAGTGGCAGA AAGGTGTCCC 
ACTCTGTATT CAAGGGAACA GTTATACAGA TATGGCATTG TGAAGAACCA TATTGGAGTC 
AGCAGCGAGA ACGCATTGTC CTTCCAGTTC CACTGTTCAA ATTATTTCAC AAAAGAACTT 
CTTCTTTCTG GAAATGGTGT GCAGTTGGCA GATGGAGGTT GGCTTATTCC CACTGAACAA 
GGCAACGCAG GCAAAGAAGA GATATACAGA GCGTTTTGTG ACACTCCTGG TGTGGATCCA 
AAATTAATTT CAGCTGAATG GGTTCATAAC CACTACCGCT GGATTGTATG GAAACTTGCT 
GCAATGGAAG TCAGGTTCCC AAAGACATTT GCTTGCAGAT GCCTTACACC AGAACGTGTG 
CTTTTACAGT TAAAGTACAG ATATGATGTG GAAATTGATA AGAGTCAGAG ATCCGCCATC 
AAAAAGATCA TGGAGAGAGA TGACTCCCCA GCTAAAACGC TGGTACTGTG CATTGCCAAG 
ATCATTTCCC AGGGCACTCG CCTGCCCAAT GCATGTAGTA ATAAAACCGA ACCCGCTGAC 
TCTAAGGAAA GTTCTGCAGT AATAGAGGTG ACAGATAGTT GGTATGGTAT CAAGGTTCTG 
CTTGATCCGT GTCTCACTGC TCTCTTACAC AAAGGAAGGC TTTTTATTGG TCAGAAGCTA 
ATTGTTCATG GAGCGGAACT TATTGGCTCA GATGATGCCT GCTCTCCCCT TGAAGCGCCA 
GAATCTCTTA TGTTGAAGAT TGCAGCTAAC AGCACTCGGC CTGTTCGATG GCATACCAAA 
CTTGGATATT TCAAGGATCC CAGACCATTC TGTCTCCATT TATCATCACT GTTAAGTGAA 
GGAGGTGTGG TGGGGTGTGT CGATGTAGTA ATACAAAGAA TCTATCCCAT GCAGTGGATG 
GAAAAAATGG CAAATGGACT GTATGTGTTT CGCAATGATC GAGCAGAGGA AAGAGAAGCA 
GAAAAGCATT CAGCAAATCA ACAAAAAAAG CTTGAAATGC TCTTTTCAAA AATACAAGCT 
GAATTTGAAC AGCGGGAAGT AACATGCAAC AGAAGAAAGG GACTCAGGCG GCGGTCATTA 
AATGCACAAC AAATGCAGAC CCTTCAGGAT GGGGCTGAAA TCTATGAAGC CATTCAGAAC 
GAATCTGACC CTGGGTATCT GGAGTCGTAC TTAAGTGCTG AGCAGCTGAA AGCCCTCAAT 
CACCACAGGC AACTTTTAAA CGATAAGAAG CAAGCACTGA TCCAAGCTGA ATTCAGAAAA 
GCCATTGAAT GCAGTGAGCA GGATGCAAAT GGCTGTACAA GGAGGGATGT CACTCCTGTA 
TGGAAATTAC GCATTGCAGA TTACAGAAAT TATGAAACAG ATGCAGCATA TATACTGAAC 
ATTTGGCGTC CGTTACCAGA TGTGCTTTCT CTTCTGAAAG AAGGTTGTCG CTATAAAATG 
TATCATTTGG CAGCATCCAC ATCAAAAGGA AAGTCATTAG CAGCTGATTT GCAGTTAACA 
GCTACAAAGA AAACTCGCTT CCAGCAACTT CAGCCATCAG AGAGCATTTT GGAGCAGATT 
TACAGCCCGA GAGAAGTGAC GGACTTCAGC AGGTTCCAGG AGCCTTTGTT TAGCGCCCCA 
TATGCAGAGG TTGATCTGGT GGGATTAATC ATCTCCATAT ACAAAAAAAC AGGTGCAGCT 
CCAGTGGTTT ATATATCAGA TGAATCTCAC AATATTGTGG CGCTAAAATT CTGGACAGAT 
CTAGGGCAGC TTGGGCTTGA GGAGATAACC AAGCCACGCA CATATATCTC TGCCAGTAAT 
CTTCGCTGGA GATCAGACTG TATCGAGGGG ATTCCTACAC TGTACGTTGG AGACTTAGCC 
AACATTTCTT CCAATCCCAA AGAAAGCCAT CTGCAGAGGG CAATTCAGAA ACTGAAACTT 
TCTGTACAGA ATGTCCAAGA TTTTTGGAAT TCATCACAAA CCGCACTGAT GAAAACCTTG 
CAGATAAATA GCACAGACAC AACGGAGTGC AGTAAGAACC CAACGACACC AACATGGAAG 
TCAGATGTTT CTGCTAGAAG TGGTTACCTA ACCCCCTTGC ACCATAGTGG TAAAAGGTTA 
TTAAATTCTG TCCATACATC AGATCCTCAG ACAGAAAATC CTGGATGTAG TAAAGAAATA 
CAACTAAAAA CCTGCAAAAA GAGGAAAGCT CTGGACTTTC TCAACCGCAT TCCTTCACCT 
CCACCTGTAA CACCAGTACG GCCCTTTGTA TCCCCATCTT TGCAGAAGGC ATTCCGACCC 
CCACGGAGCT GTAGCGTCCA GAAGTTGGGC CCGGAAACCA AGGGTAATAC TGAGAATGTC 
CAGGGAACAA CCCCAGAATG CACTAAAGAC CTAGCAAAAT TAGAGGGTGA ATTTGTGGCT 
GATGAAGAAC TCGCCATGAT CAATACCCAA GCTTTGCTTT TAGGTCTGGA GGAAGAAAAA 
AAGAAAACCG AGCAGAAAAC CAGCAGAACA GCAGGTAAAA TGACTGCCCA TGAGTCTCCA 
ATAGAAAATG CTTCCCCCGT TCCTGCCCAG GAACAACAAA CTGAGGAAGC ATTGAACATT 
CCAGTCGGTA ACTCAGAGAA ATCTTACCTG TGCCTAAGAA AAAGGAAACG GAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947219.2
CDS	280..9936
Translation	MAAPQLGKSVFYDLFSTHCSHSDLGPISLNWFEELTAEALPYKSRTCEDHECSLDDLHENNIKTPKLKSSIYSQLDSTPVIFKERLLSPLFASSLTELDKRQNATDNGNNVRLEKSNCTQMQHQASEVFSPSSRCLNESPAVIKEIFKTPLRNKYLHKTPQCDWKPDICSSLFCTPKLMKNQTGCIKESLGAEVDPEMSWSSSLATPPSPTVIIAHANDQPSGNKHAAIVQSLFPNCENMEANNLPPPETGTQNEDRQICAGLEKPGSCSASKTVPEASAISSRAAWKKTVANTVKDEEVSRTVENALEGMEDVLSIFFASEKTPGLRKLKNSTQARRKVESIKSQKCQVSLFKAEEGESILVPLDEHHKCDHEMLMKPEQDYKMNAVNQKIAKETTPYEWSQLNICELDITQSQDSTLHAANLCCEDITVNKSDTCNITEAEKQEEVNEPSTDNVLSNKVGKESTLNVNCINADLNNMASSSNNSVHSLNLSQCEKMDSSEISNSEVGCITKLTTHPAKITTGELSSVDDCAKKPQERVTISTSFSTLKKQSKFMYSVNTVLTGHIASNTSIATRRSLNSHLSSDEPKPHIKENQNEPESNAKYYSNGLQKPVFLEKENDNKGISLCKTISDGQNTSEGARAPDYFEERVPETKDRCKATLSIREKVVATANCFARKQLETDYPEDASAQHEALQFHAKQYVSCSMLNSNVSNDMQIKPQVLTQACDFRSSAENLVEKTKNQLRRNEDQSFSSTGIGKKPASGEKLVGDCNEGSFLHVKQEISAAQALVGNGYETRDTPDVTSDHTRSVISNHGKSLWEEETALTELPVPEILSKTFKGFKTASNKKIHISDKNIVKGCDLFKEIEFGIGDAKPCNEENKEPLKKTPFDVPGYETNLKGFKTASNKEINVSENKFAKGRLLFKDIEEESGQTTAIMAKDNELFIKPASSNKPDIFKSTCIKGMNPSENLDSMPPKKGDVQQSAGLKHSDQIDVFGFDDPPAKGNVQTLGYFNMSASETDFKGFKTASNKDIIISESTLAKGKLIFEDIEDTRYSETGRTIDCKAKGGALSTSNLVMHNTMCKNEPSTSENTNDKAPKRAKVQTPMAENSHLKEPSSDKNAMDAANHQMTLKASAYSPKAASSALPRYKKKPISTTKTSFQLNEHLTESQQAEISELSSILENADSQFDFTQFRKVPSVTEKQNSTEGGSESQNLNNSDVWKDVDFNDSFAAGRDHSEGMEAIPSSPGIKELPSAGECCSKETHDSMPDFVTLAPKQGFLVKQNERLFAGFNLASGKQVNIDNDVLKKAAELFNDIDNDKELLSHAKEESRKSNIKHSSKLINNENCGKTEHTSEHLVCQSNVSLPFIKTLGKNATILVSGNEKSQNQELQLDCLKCESTDVKHTPQKETINDNSKCNESSLSELSMKGFQTASGRNIMMSESSIQKARNIFAEEHEDSFTLRCNIQNTIQIPQPVNEPTQFPYVNLGPKPTTTSGWQEKNILRRSTEKGFMPGFCTAGGKKVSVSDDSLAKAHKLFQEECTFSKEGKLDEVKQNKLMNSEPLSLLTCESVLKQSDGFIEDISTSRNALEIRPELYPEGMCSNRASNGSGNNSEFTAGEGISININQSSLLTTGNVLKNLPSESSGHDVYSVTEHLSTVVKVKRYNDSGHFVNQNLAECNDNHVLSTQKNTANISNRNEDCTSLAPLSFSTASGKSVTVSHDSLQKARLMLSEAANDVTVDTSKQEAAYITPAIRKTEAEKEQNTVDDSDRVNANTFSFSTASGKKVNISGNSLKQVRAVCLSSDPKETSAALFNVEKSVFNEDVKDVSLLQPNVTMPKAVSFSTASGKTVQLSDESLKKARVIFSEIDTCPLMQQQTNESTVEEIVIGGGMTKSKQMPLTTEKVETTRKNNGTFGFNTASGKQVSVSESALQKVKDIFQEFDDPDNYEQNKSLVRLPVSSKIKESTPGTKRLVQTAGSSYKNDNLQCKAGNLRTFQDKQAGKKSLTYSEAAISPIITTVPGNQLTPIQLVKVSTNTSALANKTKPDLYVATAQNTPQNDFEIEAAESARAFLEDDDLTDMGSAAHETYLYLNQTSNTRTGKRSRTEGPTPGEPPIKRRLLPEFDCTTDTRQQTGILKPLISTPDMMRDRRRFLYTLPLKPSTCNPESALRQVTCIPTQAHLHSKVKIFHQSLPIKSPDVASDSTSKSYSPTAAKETINCSSASKIPAKKFVPPFKKTVATLADNQSNSVQNGSSDGLIESIVYPKEDKVETICSSKDQFDDSDILQMTSNLRCSKDLQEMRIRKKLRQKIKPHPGSLYRLKMSHVKRISLQSAVAERCPTLYSREQLYRYGIVKNHIGVSSENALSFQFHCSNYFTKELLLSGNGVQLADGGWLIPTEQGNAGKEEIYRAFCDTPGVDPKLISAEWVHNHYRWIVWKLAAMEVRFPKTFACRCLTPERVLLQLKYRYDVEIDKSQRSAIKKIMERDDSPAKTLVLCIAKIISQGTRLPNACSNKTEPADSKESSAVIEVTDSWYGIKVLLDPCLTALLHKGRLFIGQKLIVHGAELIGSDDACSPLEAPESLMLKIAANSTRPVRWHTKLGYFKDPRPFCLHLSSLLSEGGVVGCVDVVIQRIYPMQWMEKMANGLYVFRNDRAEEREAEKHSANQQKKLEMLFSKIQAEFEQREVTCNRRKGLRRRSLNAQQMQTLQDGAEIYEAIQNESDPGYLESYLSAEQLKALNHHRQLLNDKKQALIQAEFRKAIECSEQDANGCTRRDVTPVWKLRIADYRNYETDAAYILNIWRPLPDVLSLLKEGCRYKMYHLAASTSKGKSLAADLQLTATKKTRFQQLQPSESILEQIYSPREVTDFSRFQEPLFSAPYAEVDLVGLIISIYKKTGAAPVVYISDESHNIVALKFWTDLGQLGLEEITKPRTYISASNLRWRSDCIEGIPTLYVGDLANISSNPKESHLQRAIQKLKLSVQNVQDFWNSSQTALMKTLQINSTDTTECSKNPTTPTWKSDVSARSGYLTPLHHSGKRLLNSVHTSDPQTENPGCSKEIQLKTCKKRKALDFLNRIPSPPPVTPVRPFVSPSLQKAFRPPRSCSVQKLGPETKGNTENVQGTTPECTKDLAKLEGEFVADEELAMINTQALLLGLEEEKKKTEQKTSRTAGKMTAHESPIENASPVPAQEQQTEEALNIPVGNSEKSYLCLRKRKRK

Target ORF information:

RefSeq Version	XM_018091730.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis BRCA2, DNA repair associated (brca2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091730.2

ORF Insert Sequence:

ATGGCTGCAC CGCAACTTGG AAAATCTGTC TTCTATGATC TGTTTAGCAC GCATTGCTCT 
CACTCAGATT TAGGGCCTAT CAGTCTAAAC TGGTTTGAAG AGCTCACTGC TGAAGCCTTG 
CCTTACAAGT CAAGAACATG TGAAGATCAT GAATGCAGTT TGGATGATCT CCATGAAAAC 
AACATCAAAA CTCCTAAACT GAAGTCGTCA ATTTATAGCC AATTAGATTC CACTCCAGTC 
ATCTTTAAGG AGCGTTTACT ATCACCTCTT TTTGCTTCTT CTTTGACAGA GCTGGATAAA 
AGGCAGAATG CAACAGATAA TGGAAACAAT GTTAGACTTG AGAAAAGTAA CTGTACCCAA 
ATGCAGCATC AAGCAAGTGA AGTTTTCAGT CCCTCAAGTC GATGCCTCAA CGAAAGTCCT 
GCTGTTATCA AAGAAATATT CAAGACACCA TTACGAAATA AGTACCTACA TAAAACACCA 
CAGTGTGATT GGAAACCTGA CATTTGCAGC AGTCTGTTTT GTACTCCAAA GCTTATGAAG 
AACCAAACTG GATGTATTAA AGAAAGTCTA GGTGCAGAGG TGGATCCAGA GATGTCATGG 
TCTAGTTCAC TAGCTACACC TCCTTCCCCA ACTGTGATAA TTGCACATGC TAATGATCAG 
CCCAGTGGAA ATAAACATGC TGCAATTGTA CAGAGCCTTT TCCCAAATTG TGAGAATATG 
GAAGCGAATA ATTTGCCACC ACCTGAGACG GGCACACAAA ATGAAGACCG TCAAATATGT 
GCAGGACTAG AAAAGCCAGG TTCATGCAGT GCGAGCAAAA CTGTACCAGA AGCGTCTGCC 
ATTTCCTCAA GGGCAGCCTG GAAGAAAACT GTTGCAAATA CAGTGAAGGA CGAGGAGGTT 
TCCAGAACTG TGGAAAATGC TCTGGAAGGA ATGGAAGATG TCCTCTCAAT CTTTTTTGCA 
AGTGAAAAGA CTCCTGGTCT CAGAAAGCTG AAAAATTCTA CCCAAGCTAG GAGAAAAGTG 
GAATCTATTA AGTCTCAGAA GTGCCAAGTT TCATTATTTA AAGCAGAAGA AGGAGAAAGC 
ATTTTGGTCC CTTTAGATGA GCATCATAAA TGTGATCATG AGATGCTTAT GAAACCTGAA 
CAAGACTACA AAATGAATGC TGTTAACCAA AAAATAGCCA AAGAAACTAC TCCATATGAA 
TGGTCACAGC TAAATATATG TGAGTTGGAT ATAACTCAAT CACAGGATAG CACTTTGCAT 
GCAGCAAATC TATGTTGTGA AGATATCACT GTCAACAAAA GTGATACATG TAATATTACT 
GAAGCCGAAA AGCAGGAGGA AGTCAATGAG CCAAGCACCG ACAATGTACT GTCTAATAAA 
GTTGGCAAAG AAAGCACTTT AAATGTGAAT TGTATTAATG CTGATTTGAA TAACATGGCT 
TCATCCTCAA ATAACTCTGT TCACTCACTG AATCTTTCTC AATGTGAAAA AATGGATTCT 
TCTGAAATTT CTAACAGTGA AGTTGGTTGT ATAACAAAAC TAACAACCCA CCCAGCAAAA 
ATAACAACTG GTGAATTAAG TTCAGTAGAT GACTGTGCTA AGAAACCCCA GGAAAGAGTA 
ACTATTAGTA CTTCATTTTC AACTCTGAAA AAGCAATCGA AGTTTATGTA CAGTGTAAAC 
ACTGTGTTAA CTGGTCATAT TGCAAGCAAT ACCAGCATAG CCACTAGGAG ATCATTGAAC 
AGTCATTTAA GTTCTGATGA ACCAAAACCT CACATTAAGG AAAATCAAAA TGAACCAGAA 
TCAAATGCTA AATATTATTC CAACGGCCTG CAGAAACCAG TATTCCTGGA AAAGGAGAAT 
GATAATAAGG GAATTTCTTT ATGTAAAACT ATCTCAGACG GTCAAAACAC TTCAGAAGGT 
GCCAGGGCTC CAGACTATTT TGAGGAACGT GTTCCTGAAA CAAAAGATAG ATGCAAAGCT 
ACACTAAGCA TCAGGGAAAA GGTTGTGGCT ACAGCAAATT GTTTTGCAAG AAAACAGCTT 
GAAACAGATT ATCCAGAGGA TGCCTCAGCA CAACATGAGG CACTTCAGTT CCATGCTAAG 
CAATATGTGT CATGCTCAAT GCTAAATTCT AATGTGTCCA ATGACATGCA AATAAAGCCT 
CAAGTGTTAA CTCAGGCATG CGATTTTAGG AGCAGCGCAG AAAATCTTGT TGAAAAAACA 
AAAAATCAAT TAAGGCGGAA TGAAGATCAA TCATTTTCAT CAACTGGCAT AGGGAAAAAG 
CCAGCTAGTG GTGAAAAATT AGTAGGTGAC TGTAATGAGG GCTCATTCCT TCATGTGAAG 
CAAGAAATAA GTGCTGCTCA GGCATTGGTA GGTAATGGTT ACGAAACCAG AGACACTCCT 
GATGTCACTA GTGATCATAC AAGGAGTGTT ATTTCCAACC ATGGAAAGTC TCTGTGGGAG 
GAGGAGACAG CACTGACTGA ATTACCAGTC CCAGAAATTC TGAGTAAAAC TTTCAAGGGC 
TTTAAAACGG CATCTAATAA AAAAATACAT ATTTCAGATA AAAATATTGT AAAAGGTTGT 
GACTTGTTCA AAGAAATTGA GTTTGGCATT GGTGACGCTA AGCCCTGCAA TGAAGAAAAC 
AAAGAGCCCT TAAAGAAAAC TCCCTTTGAT GTTCCTGGGT ATGAAACAAA TTTAAAAGGA 
TTCAAAACTG CATCCAATAA AGAAATAAAT GTATCAGAAA ACAAATTTGC TAAAGGTAGA 
CTGCTGTTTA AAGACATCGA AGAGGAAAGC GGTCAAACTA CAGCTATAAT GGCAAAAGAT 
AATGAGCTGT TCATTAAGCC TGCATCCAGC AATAAGCCTG ACATATTTAA GAGTACATGT 
ATAAAAGGAA TGAATCCATC TGAGAACCTA GACAGTATGC CCCCCAAAAA AGGAGACGTG 
CAGCAGAGTG CAGGTTTAAA ACATTCAGAT CAAATTGACG TTTTTGGTTT CGATGATCCT 
CCTGCAAAAG GAAATGTTCA AACATTAGGC TATTTTAATA TGTCTGCTTC TGAAACAGAC 
TTCAAAGGAT TCAAAACCGC TTCCAATAAA GACATAATTA TTTCAGAAAG CACACTTGCT 
AAAGGTAAAC TTATTTTTGA AGATATTGAA GATACAAGAT ACAGTGAAAC AGGAAGGACC 
ATAGATTGTA AAGCAAAAGG TGGTGCGTTG TCCACCAGTA ACCTTGTAAT GCATAACACC 
ATGTGTAAAA ATGAACCAAG TACATCTGAG AACACAAATG ATAAAGCACC AAAAAGAGCA 
AAAGTACAAA CACCTATGGC AGAAAATAGT CATTTAAAAG AACCTTCTTC AGACAAAAAT 
GCAATGGATG CTGCAAATCA TCAAATGACA CTGAAAGCCT CCGCATACAG CCCAAAGGCT 
GCCTCTTCTG CACTGCCCAG GTACAAAAAG AAACCAATCT CTACCACTAA AACCTCTTTC 
CAGTTGAATG AACATTTGAC TGAAAGCCAG CAAGCTGAAA TCTCTGAACT TTCCTCTATT 
CTAGAAAATG CAGACAGTCA GTTTGATTTT ACTCAGTTTA GGAAAGTGCC TTCTGTGACT 
GAGAAACAAA ATTCAACTGA AGGTGGGAGC GAGTCCCAGA ACCTTAACAA CTCAGATGTC 
TGGAAGGACG TAGATTTTAA TGATAGTTTT GCAGCAGGAA GGGATCATTC TGAGGGAATG 
GAGGCAATTC CCTCCAGTCC TGGAATAAAA GAATTGCCTT CTGCAGGAGA ATGTTGCAGT 
AAAGAAACAC ATGATTCTAT GCCAGATTTT GTGACCTTGG CACCTAAACA AGGTTTTCTG 
GTTAAACAAA ATGAAAGACT CTTTGCAGGG TTTAATCTTG CTAGTGGCAA GCAAGTTAAT 
ATAGACAATG ATGTACTAAA AAAAGCTGCT GAACTGTTCA ATGATATAGA TAATGATAAA 
GAATTGCTCA GCCACGCCAA AGAAGAAAGC AGAAAAAGCA ATATAAAACA CTCTTCTAAA 
TTAATAAATA ATGAAAATTG TGGAAAGACA GAACACACAT CTGAGCATTT GGTATGTCAA 
AGTAATGTTT CATTACCATT CATAAAAACA TTGGGCAAGA ATGCTACAAT ACTAGTGAGT 
GGTAATGAAA AAAGCCAGAA TCAAGAATTA CAGCTGGATT GTTTAAAATG TGAAAGTACT 
GATGTAAAGC ATACACCACA AAAGGAAACA ATCAATGACA ACTCAAAGTG TAATGAATCC 
TCTCTTTCTG AGCTTTCCAT GAAAGGTTTT CAGACTGCCA GTGGGAGAAA CATTATGATG 
TCCGAGTCTT CAATCCAGAA AGCCAGAAAC ATATTTGCAG AGGAACATGA AGACAGTTTT 
ACATTGCGCT GTAATATTCA AAATACAATA CAAATACCAC AGCCTGTAAA TGAACCGACC 
CAATTCCCAT ATGTTAATTT GGGGCCAAAA CCCACAACGA CATCAGGATG GCAAGAAAAA 
AATATTCTGA GAAGAAGTAC AGAAAAAGGT TTCATGCCGG GTTTTTGCAC TGCCGGTGGT 
AAAAAGGTTT CTGTTTCCGA CGACTCTCTA GCTAAAGCAC ATAAACTTTT CCAGGAGGAA 
TGTACCTTTA GCAAGGAAGG GAAATTGGAT GAGGTGAAAC AAAATAAATT AATGAACTCT 
GAGCCATTGT CTCTTCTCAC TTGTGAGTCA GTCCTGAAAC AGAGTGACGG ATTCATTGAA 
GATATTTCAA CTTCTAGAAA TGCTTTGGAA ATCAGACCAG AGCTTTATCC GGAGGGCATG 
TGTAGTAATA GGGCATCTAA TGGAAGTGGA AACAATAGTG AATTTACAGC TGGGGAAGGC 
ATATCAATAA ATATCAACCA ATCATCGCTG CTTACAACAG GCAACGTTTT AAAAAATTTG 
CCGTCTGAAA GTAGTGGCCA TGATGTGTAT TCAGTAACTG AGCATCTAAG CACTGTGGTT 
AAAGTCAAAA GATACAATGA TTCAGGTCAT TTTGTAAATC AAAATTTAGC TGAATGTAAT 
GATAACCATG TCCTTAGCAC TCAAAAAAAT ACTGCAAATA TAAGCAACAG AAACGAGGAC 
TGTACATCCC TGGCACCTCT CTCTTTCAGC ACAGCCAGTG GGAAATCCGT CACAGTGTCT 
CATGATTCTC TTCAAAAAGC AAGACTGATG CTTTCTGAAG CAGCTAATGA TGTTACAGTA 
GATACTAGTA AGCAAGAGGC GGCTTATATT ACGCCAGCCA TTCGAAAAAC AGAAGCAGAA 
AAAGAGCAGA ACACTGTTGA TGATTCTGAT AGAGTTAATG CAAATACATT TTCATTTTCT 
ACTGCAAGCG GTAAAAAGGT CAACATAAGC GGAAATTCTT TAAAGCAGGT CAGAGCTGTC 
TGCCTATCCT CTGACCCAAA AGAAACATCA GCGGCTCTGT TTAATGTTGA AAAAAGTGTT 
TTTAATGAAG ATGTTAAGGA TGTATCTTTG CTTCAGCCCA ATGTTACCAT GCCGAAGGCT 
GTGTCGTTTA GTACTGCAAG TGGCAAGACG GTGCAGTTGT CAGACGAGTC TTTGAAGAAA 
GCTAGGGTGA TTTTTTCTGA AATAGATACT TGTCCGTTGA TGCAACAGCA GACTAATGAA 
AGCACTGTTG AAGAGATAGT AATAGGAGGA GGAATGACAA AATCAAAACA GATGCCATTA 
ACAACAGAAA AAGTCGAAAC AACTAGAAAA AATAATGGTA CTTTTGGCTT TAATACCGCA 
AGCGGAAAGC AGGTCTCTGT GTCTGAAAGT GCGCTGCAGA AAGTCAAAGA TATCTTTCAA 
GAATTTGATG ATCCCGACAA TTATGAACAA AATAAATCAC TCGTAAGACT GCCTGTTTCA 
TCAAAAATAA AAGAATCAAC TCCAGGAACA AAAAGACTGG TGCAAACTGC AGGGTCCTCT 
TATAAAAATG ATAATTTGCA ATGTAAAGCA GGGAATCTTC GTACTTTTCA GGATAAGCAA 
GCTGGCAAGA AGTCACTTAC ATATTCAGAA GCAGCCATAA GCCCCATTAT AACAACTGTC 
CCTGGAAATC AATTAACGCC CATCCAGTTG GTAAAAGTCT CAACCAACAC ATCAGCATTG 
GCTAATAAAA CCAAACCCGA TTTATATGTC GCAACAGCCC AGAACACACC ACAAAATGAT 
TTTGAAATAG AAGCAGCAGA AAGTGCTAGA GCTTTCTTGG AAGATGATGA CCTGACAGAT 
ATGGGATCAG CTGCCCATGA AACATACTTG TATCTCAACC AGACCTCAAA TACAAGAACT 
GGGAAGAGAT CAAGAACAGA AGGGCCGACA CCTGGAGAGC CACCAATCAA AAGACGGCTT 
TTACCAGAAT TTGATTGCAC AACAGACACT CGGCAACAAA CGGGGATTTT AAAGCCTTTG 
ATCAGCACTC CAGACATGAT GCGAGACAGG CGAAGGTTTT TGTACACCCT TCCCTTGAAG 
CCATCAACTT GTAATCCTGA AAGTGCTTTA CGTCAAGTCA CGTGCATTCC TACACAAGCC 
CATCTCCATT CTAAAGTCAA AATCTTCCAC CAGTCTCTAC CCATCAAGTC ACCAGATGTT 
GCATCTGATT CAACTTCTAA ATCGTATAGC CCCACTGCTG CAAAAGAGAC CATAAACTGC 
TCATCTGCAT CTAAAATACC TGCAAAGAAA TTTGTGCCTC CTTTTAAAAA GACTGTGGCG 
ACCTTGGCAG ACAACCAGTC AAACAGTGTA CAAAATGGCA GTTCGGATGG GCTTATCGAG 
AGCATTGTTT ATCCAAAAGA AGACAAAGTT GAAACCATAT GTTCTTCAAA AGATCAATTT 
GATGACTCAG ATATTTTACA AATGACTTCA AATCTTCGAT GTAGCAAAGA CCTGCAGGAA 
ATGAGAATTC GGAAAAAGCT AAGGCAAAAG ATTAAACCGC ATCCAGGCTC ACTGTATCGT 
CTGAAAATGT CACATGTGAA GAGAATTTCA CTTCAATCTG CAGTGGCAGA AAGGTGTCCC 
ACTCTGTATT CAAGGGAACA GTTATACAGA TATGGCATTG TGAAGAACCA TATTGGAGTC 
AGCAGCGAGA ACGCATTGTC CTTCCAGTTC CACTGTTCAA ATTATTTCAC AAAAGAACTT 
CTTCTTTCTG GAAATGGTGT GCAGTTGGCA GATGGAGGTT GGCTTATTCC CACTGAACAA 
GGCAACGCAG GCAAAGAAGA GATATACAGA GCGTTTTGTG ACACTCCTGG TGTGGATCCA 
AAATTAATTT CAGCTGAATG GGTTCATAAC CACTACCGCT GGATTGTATG GAAACTTGCT 
GCAATGGAAG TCAGGTTCCC AAAGACATTT GCTTGCAGAT GCCTTACACC AGAACGTGTG 
CTTTTACAGT TAAAGTACAG ATATGATGTG GAAATTGATA AGAGTCAGAG ATCCGCCATC 
AAAAAGATCA TGGAGAGAGA TGACTCCCCA GCTAAAACGC TGGTACTGTG CATTGCCAAG 
ATCATTTCCC AGGGCACTCG CCTGCCCAAT GCATGTAGTA ATAAAACCGA ACCCGCTGAC 
TCTAAGGAAA GTTCTGCAGT AATAGAGGTG ACAGATAGTT GGTATGGTAT CAAGGTTCTG 
CTTGATCCGT GTCTCACTGC TCTCTTACAC AAAGGAAGGC TTTTTATTGG TCAGAAGCTA 
ATTGTTCATG GAGCGGAACT TATTGGCTCA GATGATGCCT GCTCTCCCCT TGAAGCGCCA 
GAATCTCTTA TGTTGAAGAT TGCAGCTAAC AGCACTCGGC CTGTTCGATG GCATACCAAA 
CTTGGATATT TCAAGGATCC CAGACCATTC TGTCTCCATT TATCATCACT GTTAAGTGAA 
GGAGGTGTGG TGGGGTGTGT CGATGTAGTA ATACAAAGAA TCTATCCCAT GCAGTGGATG 
GAAAAAATGG CAAATGGACT GTATGTGTTT CGCAATGATC GAGCAGAGGA AAGAGAAGCA 
GAAAAGCATT CAGCAAATCA ACAAAAAAAG CTTGAAATGC TCTTTTCAAA AATACAAGCT 
GAATTTGAAC AGCGGGAAGT AACATGCAAC AGAAGAAAGG GACTCAGGCG GCGGTCATTA 
AATGCACAAC AAATGCAGAC CCTTCAGGAT GGGGCTGAAA TCTATGAAGC CATTCAGAAC 
GAATCTGACC CTGGGTATCT GGAGTCGTAC TTAAGTGCTG AGCAGCTGAA AGCCCTCAAT 
CACCACAGGC AACTTTTAAA CGATAAGAAG CAAGCACTGA TCCAAGCTGA ATTCAGAAAA 
GCCATTGAAT GCAGTGAGCA GGATGCAAAT GGCTGTACAA GGAGGGATGT CACTCCTGTA 
TGGAAATTAC GCATTGCAGA TTACAGAAAT TATGAAACAG ATGCAGCATA TATACTGAAC 
ATTTGGCGTC CGTTACCAGA TGTGCTTTCT CTTCTGAAAG AAGGTTGTCG CTATAAAATG 
TATCATTTGG CAGCATCCAC ATCAAAAGGA AAGTCATTAG CAGCTGATTT GCAGTTAACA 
GCTACAAAGA AAACTCGCTT CCAGCAACTT CAGCCATCAG AGAGCATTTT GGAGCAGATT 
TACAGCCCGA GAGAAGTGAC GGACTTCAGC AGGTTCCAGG AGCCTTTGTT TAGCGCCCCA 
TATGCAGAGG TTGATCTGGT GGGATTAATC ATCTCCATAT ACAAAAAAAC AGGTGCAGCT 
CCAGTGGTTT ATATATCAGA TGAATCTCAC AATATTGTGG CGCTAAAATT CTGGACAGAT 
CTAGGGCAGC TTGGGCTTGA GGAGATAACC AAGCCACGCA CATATATCTC TGCCAGTAAT 
CTTCGCTGGA GATCAGACTG TATCGAGGGG ATTCCTACAC TGTACGTTGG AGACTTAGCC 
AACATTTCTT CCAATCCCAA AGAAAGCCAT CTGCAGAGGG CAATTCAGAA ACTGAAACTT 
TCTGTACAGA ATGTCCAAGA TTTTTGGAAT TCATCACAAA CCGCACTGAT GAAAACCTTG 
CAGATAAATA GCACAGACAC AACGGAGTGC AGTAAGAACC CAACGACACC AACATGGAAG 
TCAGATGTTT CTGCTAGAAG TGGTTACCTA ACCCCCTTGC ACCATAGTGG TAAAAGGTTA 
TTAAATTCTG TCCATACATC AGATCCTCAG ACAGAAAATC CTGGATGTAG TAAAGAAATA 
CAACTAAAAA CCTGCAAAAA GAGGAAAGCT CTGGACTTTC TCAACCGCAT TCCTTCACCT 
CCACCTGTAA CACCAGTACG GCCCTTTGTA TCCCCATCTT TGCAGAAGGC ATTCCGACCC 
CCACGGAGCT GTAGCGTCCA GAAGTTGGGC CCGGAAACCA AGGGTAATAC TGAGAATGTC 
CAGGGAACAA CCCCAGAATG CACTAAAGAC CTAGCAAAAT TAGAGGGTGA ATTTGTGGCT 
GATGAAGAAC TCGCCATGAT CAATACCCAA GCTTTGCTTT TAGGTCTGGA GGAAGAAAAA 
AAGAAAACCG AGCAGAAAAC CAGCAGAACA GCAGGTAAAA TGACTGCCCA TGAGTCTCCA 
ATAGAAAATG CTTCCCCCGT TCCTGCCCAG GAACAACAAA CTGAGGAAGC ATTGAACATT 
CCAGTCGGTA ACTCAGAGAA ATCTTACCTG TGCCTAAGAA AAAGGAAACG GAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

brca2 ( XM_031897237.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031753097.1 Xenopus tropicalis BRCA2, DNA repair associated (brca2), transcript variant X5, mRNA.

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CloneID	OXa52808
Clone ID Related Accession (Same CDS sequence)	XM_031897237.1
Accession Version	XM_031897237.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	breast cancer type 2 susceptibility protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAC CGCAACTTGG AAAATCTGTC TTCTATGATC TGTTTAGCAC GCATTGCTCT 
CACTCAGATT TAGGGCCTAT CAGTCTAAAC TGGTTTGAAG AGCTCACTGC TGAAGCCTTG 
CCTTACAAGT CAAGAACATG TGAAGATCAT GAATGCAGTT TGGATGATCT CCATGAAAAC 
AACATCAAAA CTCCTAAACT GAAGTCGTCA ATTTATAGCC AATTAGATTC CACTCCAGTC 
ATCTTTAAGG AGCGTTTACT ATCACCTCTT TTTGCTTCTT CTTTGACAGA GCTGGATAAA 
AGGCAGAATG CAACAGATAA TGGAAACAAT GTTAGACTTG AGAAAAGTAA CTGTACCCAA 
ATGCAGCATC AAGCAAGTGA AGTTTTCAGT CCCTCAAGTC GATGCCTCAA CGAAAGTCCT 
GCTGTTATCA AAGAAATATT CAAGACACCA TTACGAAATA AGTACCTACA TAAAACACCA 
CAGTGTGATT GGAAACCTGA CATTTGCAGC AGTCTGTTTT GTACTCCAAA GCTTATGAAG 
AACCAAACTG GATGTATTAA AGAAAGTCTA GGTGCAGAGG TGGATCCAGA GATGTCATGG 
TCTAGTTCAC TAGCTACACC TCCTTCCCCA ACTGTGATAA TTGCACATGC TAATGATCAG 
CCCAGTGGAA ATAAACATGC TGCAATTGTA CAGAGCCTTT TCCCAAATTG TGAGAATATG 
GAAGCGAATA ATTTGCCACC ACCTGAGACG GGCACACAAA ATGAAGACCG TCAAATATGT 
GGACTAGAAA AGCCAGGTTC ATGCAGTGCG AGCAAAACTG TACCAGAAGC GTCTGCCATT 
TCCTCAAGGG CAGCCTGGAA GAAAACTGTT GCAAATACAG TGAAGGACGA GGAGGTTTCC 
AGAACTGTGG AAAATGCTCT GGAAGGAATG GAAGATGTCC TCTCAATCTT TTTTGCAAGT 
GAAAAGACTC CTGGTCTCAG AAAGCTGAAA AATTCTACCC AAGCTAGGAG AAAAGTGGAA 
TCTATTAAGT CTCAGAAGTG CCAAGTTTCA TTATTTAAAG CAGAAGAAGG AGAAAGCATT 
TTGGTCCCTT TAGATGAGCA TCATAAATGT GATCATGAGA TGCTTATGAA ACCTGAACAA 
GACTACAAAA TGAATGCTGT TAACCAAAAA ATAGCCAAAG AAACTACTCC ATATGAATGG 
TCACAGCTAA ATATATGTGA GTTGGATATA ACTCAATCAC AGGATAGCAC TTTGCATGCA 
GCAAATCTAT GTTGTGAAGA TATCACTGTC AACAAAAGTG ATACATGTAA TATTACTGAA 
GCCGAAAAGC AGGAGGAAGT CAATGAGCCA AGCACCGACA ATGTACTGTC TAATAAAGTT 
GGCAAAGAAA GCACTTTAAA TGTGAATTGT ATTAATGCTG ATTTGAATAA CATGGCTTCA 
TCCTCAAATA ACTCTGTTCA CTCACTGAAT CTTTCTCAAT GTGAAAAAAT GGATTCTTCT 
GAAATTTCTA ACAGTGAAGT TGGTTGTATA ACAAAACTAA CAACCCACCC AGCAAAAATA 
ACAACTGGTG AATTAAGTTC AGTAGATGAC TGTGCTAAGA AACCCCAGGA AAGAGTAACT 
ATTAGTACTT CATTTTCAAC TCTGAAAAAG CAATCGAAGT TTATGTACAG TGTAAACACT 
GTGTTAACTG GTCATATTGC AAGCAATACC AGCATAGCCA CTAGGAGATC ATTGAACAGT 
CATTTAAGTT CTGATGAACC AAAACCTCAC ATTAAGGAAA ATCAAAATGA ACCAGAATCA 
AATGCTAAAT ATTATTCCAA CGGCCTGCAG AAACCAGTAT TCCTGGAAAA GGAGAATGAT 
AATAAGGGAA TTTCTTTATG TAAAACTATC TCAGACGGTC AAAACACTTC AGAAGGTGCC 
AGGGCTCCAG ACTATTTTGA GGAACGTGTT CCTGAAACAA AAGATAGATG CAAAGCTACA 
CTAAGCATCA GGGAAAAGGT TGTGGCTACA GCAAATTGTT TTGCAAGAAA ACAGCTTGAA 
ACAGATTATC CAGAGGATGC CTCAGCACAA CATGAGGCAC TTCAGTTCCA TGCTAAGCAA 
TATGTGTCAT GCTCAATGCT AAATTCTAAT GTGTCCAATG ACATGCAAAT AAAGCCTCAA 
GTGTTAACTC AGGCATGCGA TTTTAGGAGC AGCGCAGAAA ATCTTGTTGA AAAAACAAAA 
AATCAATTAA GGCGGAATGA AGATCAATCA TTTTCATCAA CTGGCATAGG GAAAAAGCCA 
GCTAGTGGTG AAAAATTAGT AGGTGACTGT AATGAGGGCT CATTCCTTCA TGTGAAGCAA 
GAAATAAGTG CTGCTCAGGC ATTGGTAGGT AATGGTTACG AAACCAGAGA CACTCCTGAT 
GTCACTAGTG ATCATACAAG GAGTGTTATT TCCAACCATG GAAAGTCTCT GTGGGAGGAG 
GAGACAGCAC TGACTGAATT ACCAGTCCCA GAAATTCTGA GTAAAACTTT CAAGGGCTTT 
AAAACGGCAT CTAATAAAAA AATACATATT TCAGATAAAA ATATTGTAAA AGGTTGTGAC 
TTGTTCAAAG AAATTGAGTT TGGCATTGGT GACGCTAAGC CCTGCAATGA AGAAAACAAA 
GAGCCCTTAA AGAAAACTCC CTTTGATGTT CCTGGGTATG AAACAAATTT AAAAGGATTC 
AAAACTGCAT CCAATAAAGA AATAAATGTA TCAGAAAACA AATTTGCTAA AGGTAGACTG 
CTGTTTAAAG ACATCGAAGA GGAAAGCGGT CAAACTACAG CTATAATGGC AAAAGATAAT 
GAGCTGTTCA TTAAGCCTGC ATCCAGCAAT AAGCCTGACA TATTTAAGAG TACATGTATA 
AAAGGAATGA ATCCATCTGA GAACCTAGAC AGTATGCCCC CCAAAAAAGG AGACGTGCAG 
CAGAGTGCAG GTTTAAAACA TTCAGATCAA ATTGACGTTT TTGGTTTCGA TGATCCTCCT 
GCAAAAGGAA ATGTTCAAAC ATTAGGCTAT TTTAATATGT CTGCTTCTGA AACAGACTTC 
AAAGGATTCA AAACCGCTTC CAATAAAGAC ATAATTATTT CAGAAAGCAC ACTTGCTAAA 
GGTAAACTTA TTTTTGAAGA TATTGAAGAT ACAAGATACA GTGAAACAGG AAGGACCATA 
GATTGTAAAG CAAAAGGTGG TGCGTTGTCC ACCAGTAACC TTGTAATGCA TAACACCATG 
TGTAAAAATG AACCAAGTAC ATCTGAGAAC ACAAATGATA AAGCACCAAA AAGAGCAAAA 
GTACAAACAC CTATGGCAGA AAATAGTCAT TTAAAAGAAC CTTCTTCAGA CAAAAATGCA 
ATGGATGCTG CAAATCATCA AATGACACTG AAAGCCTCCG CATACAGCCC AAAGGCTGCC 
TCTTCTGCAC TGCCCAGGTA CAAAAAGAAA CCAATCTCTA CCACTAAAAC CTCTTTCCAG 
TTGAATGAAC ATTTGACTGA AAGCCAGCAA GCTGAAATCT CTGAACTTTC CTCTATTCTA 
GAAAATGCAG ACAGTCAGTT TGATTTTACT CAGTTTAGGA AAGTGCCTTC TGTGACTGAG 
AAACAAAATT CAACTGAAGG TGGGAGCGAG TCCCAGAACC TTAACAACTC AGATGTCTGG 
AAGGACGTAG ATTTTAATGA TAGTTTTGCA GCAGGAAGGG ATCATTCTGA GGGAATGGAG 
GCAATTCCCT CCAGTCCTGG AATAAAAGAA TTGCCTTCTG CAGGAGAATG TTGCAGTAAA 
GAAACACATG ATTCTATGCC AGATTTTGTG ACCTTGGCAC CTAAACAAGG TTTTCTGGTT 
AAACAAAATG AAAGACTCTT TGCAGGGTTT AATCTTGCTA GTGGCAAGCA AGTTAATATA 
GACAATGATG TACTAAAAAA AGCTGCTGAA CTGTTCAATG ATATAGATAA TGATAAAGAA 
TTGCTCAGCC ACGCCAAAGA AGAAAGCAGA AAAAGCAATA TAAAACACTC TTCTAAATTA 
ATAAATAATG AAAATTGTGG AAAGACAGAA CACACATCTG AGCATTTGGT ATGTCAAAGT 
AATGTTTCAT TACCATTCAT AAAAACATTG GGCAAGAATG CTACAATACT AGTGAGTGGT 
AATGAAAAAA GCCAGAATCA AGAATTACAG CTGGATTGTT TAAAATGTGA AAGTACTGAT 
GTAAAGCATA CACCACAAAA GGAAACAATC AATGACAACT CAAAGTGTAA TGAATCCTCT 
CTTTCTGAGC TTTCCATGAA AGGTTTTCAG ACTGCCAGTG GGAGAAACAT TATGATGTCC 
GAGTCTTCAA TCCAGAAAGC CAGAAACATA TTTGCAGAGG AACATGAAGA CAGTTTTACA 
TTGCGCTGTA ATATTCAAAA TACAATACAA ATACCACAGC CTGTAAATGA ACCGACCCAA 
TTCCCATATG TTAATTTGGG GCCAAAACCC ACAACGACAT CAGGATGGCA AGAAAAAAAT 
ATTCTGAGAA GAAGTACAGA AAAAGGTTTC ATGCCGGGTT TTTGCACTGC CGGTGGTAAA 
AAGGTTTCTG TTTCCGACGA CTCTCTAGCT AAAGCACATA AACTTTTCCA GGAGGAATGT 
ACCTTTAGCA AGGAAGGGAA ATTGGATGAG GTGAAACAAA ATAAATTAAT GAACTCTGAG 
CCATTGTCTC TTCTCACTTG TGAGTCAGTC CTGAAACAGA GTGACGGATT CATTGAAGAT 
ATTTCAACTT CTAGAAATGC TTTGGAAATC AGACCAGAGC TTTATCCGGA GGGCATGTGT 
AGTAATAGGG CATCTAATGG AAGTGGAAAC AATAGTGAAT TTACAGCTGG GGAAGGCATA 
TCAATAAATA TCAACCAATC ATCGCTGCTT ACAACAGGCA ACGTTTTAAA AAATTTGCCG 
TCTGAAAGTA GTGGCCATGA TGTGTATTCA GTAACTGAGC ATCTAAGCAC TGTGGTTAAA 
GTCAAAAGAT ACAATGATTC AGGTCATTTT GTAAATCAAA ATTTAGCTGA ATGTAATGAT 
AACCATGTCC TTAGCACTCA AAAAAATACT GCAAATATAA GCAACAGAAA CGAGGACTGT 
ACATCCCTGG CACCTCTCTC TTTCAGCACA GCCAGTGGGA AATCCGTCAC AGTGTCTCAT 
GATTCTCTTC AAAAAGCAAG ACTGATGCTT TCTGAAGCAG CTAATGATGT TACAGTAGAT 
ACTAGTAAGC AAGAGGCGGC TTATATTACG CCAGCCATTC GAAAAACAGA AGCAGAAAAA 
GAGCAGAACA CTGTTGATGA TTCTGATAGA GTTAATGCAA ATACATTTTC ATTTTCTACT 
GCAAGCGGTA AAAAGGTCAA CATAAGCGGA AATTCTTTAA AGCAGGTCAG AGCTGTCTGC 
CTATCCTCTG ACCCAAAAGA AACATCAGCG GCTCTGTTTA ATGTTGAAAA AAGTGTTTTT 
AATGAAGATG TTAAGGATGT ATCTTTGCTT CAGCCCAATG TTACCATGCC GAAGGCTGTG 
TCGTTTAGTA CTGCAAGTGG CAAGACGGTG CAGTTGTCAG ACGAGTCTTT GAAGAAAGCT 
AGGGTGATTT TTTCTGAAAT AGATACTTGT CCGTTGATGC AACAGCAGAC TAATGAAAGC 
ACTGTTGAAG AGATAGTAAT AGGAGGAGGA ATGACAAAAT CAAAACAGAT GCCATTAACA 
ACAGAAAAAG TCGAAACAAC TAGAAAAAAT AATGGTACTT TTGGCTTTAA TACCGCAAGC 
GGAAAGCAGG TCTCTGTGTC TGAAAGTGCG CTGCAGAAAG TCAAAGATAT CTTTCAAGAA 
TTTGATGATC CCGACAATTA TGAACAAAAT AAATCACTCG TAAGACTGCC TGTTTCATCA 
AAAATAAAAG AATCAACTCC AGGAACAAAA AGACTGGTGC AAACTGCAGG GTCCTCTTAT 
AAAAATGATA ATTTGCAATG TAAAGCAGGG AATCTTCGTA CTTTTCAGGA TAAGCAAGCT 
GGCAAGAAGT CACTTACATA TTCAGAAGCA GCCATAAGCC CCATTATAAC AACTGTCCCT 
GGAAATCAAT TAACGCCCAT CCAGTTGGTA AAAGTCTCAA CCAACACATC AGCATTGGCT 
AATAAAACCA AACCCGATTT ATATGTCGCA ACAGCCCAGA ACACACCACA AAATGATTTT 
GAAATAGAAG CAGCAGAAAG TGCTAGAGCT TTCTTGGAAG ATGATGACCT GACAGATATG 
GGATCAGCTG CCCATGAAAC ATACTTGTAT CTCAACCAGA CCTCAAATAC AAGAACTGGG 
AAGAGATCAA GAACAGAAGG GCCGACACCT GGAGAGCCAC CAATCAAAAG ACGGCTTTTA 
CCAGAATTTG ATTGCACAAC AGACACTCGG CAACAAACGG GGATTTTAAA GCCTTTGATC 
AGCACTCCAG ACATGATGCG AGACAGGCGA AGGTTTTTGT ACACCCTTCC CTTGAAGCCA 
TCAACTTGTA ATCCTGAAAG TGCTTTACGT CAAGTCACGT GCATTCCTAC ACAAGCCCAT 
CTCCATTCTA AAGTCAAAAT CTTCCACCAG TCTCTACCCA TCAAGTCACC AGATGTTGCA 
TCTGATTCAA CTTCTAAATC GTATAGCCCC ACTGCTGCAA AAGAGACCAT AAACTGCTCA 
TCTGCATCTA AAATACCTGC AAAGAAATTT GTGCCTCCTT TTAAAAAGAC TGTGGCGACC 
TTGGCAGACA ACCAGTCAAA CAGTGTACAA AATGGCAGTT CGGATGGGCT TATCGAGAGC 
ATTGTTTATC CAAAAGAAGA CAAAGTTGAA ACCATATGTT CTTCAAAAGA TCAATTTGAT 
GACTCAGATA TTTTACAAAT GACTTCAAAT CTTCGATGTA GCAAAGACCT GCAGGAAATG 
AGAATTCGGA AAAAGCTAAG GCAAAAGATT AAACCGCATC CAGGCTCACT GTATCGTCTG 
AAAATGTCAC ATGTGAAGAG AATTTCACTT CAATCTGCAG TGGCAGAAAG GTGTCCCACT 
CTGTATTCAA GGGAACAGTT ATACAGATAT GGCATTGTGA AGAACCATAT TGGAGTCAGC 
AGCGAGAACG CATTGTCCTT CCAGTTCCAC TGTTCAAATT ATTTCACAAA AGAACTTCTT 
CTTTCTGGAA ATGGTGTGCA GTTGGCAGAT GGAGGTTGGC TTATTCCCAC TGAACAAGGC 
AACGCAGGCA AAGAAGAGAT ATACAGAGCG TTTTGTGACA CTCCTGGTGT GGATCCAAAA 
TTAATTTCAG CTGAATGGGT TCATAACCAC TACCGCTGGA TTGTATGGAA ACTTGCTGCA 
ATGGAAGTCA GGTTCCCAAA GACATTTGCT TGCAGATGCC TTACACCAGA ACGTGTGCTT 
TTACAGTTAA AGTACAGATA TGATGTGGAA ATTGATAAGA GTCAGAGATC CGCCATCAAA 
AAGATCATGG AGAGAGATGA CTCCCCAGCT AAAACGCTGG TACTGTGCAT TGCCAAGATC 
ATTTCCCAGG GCACTCGCCT GCCCAATGCA TGTAGTAATA AAACCGAACC CGCTGACTCT 
AAGGAAAGTT CTGCAGTAAT AGAGGTGACA GATAGTTGGT ATGGTATCAA GGTTCTGCTT 
GATCCGTGTC TCACTGCTCT CTTACACAAA GGAAGGCTTT TTATTGGTCA GAAGCTAATT 
GTTCATGGAG CGGAACTTAT TGGCTCAGAT GATGCCTGCT CTCCCCTTGA AGCGCCAGAA 
TCTCTTATGT TGAAGATTGC AGCTAACAGC ACTCGGCCTG TTCGATGGCA TACCAAACTT 
GGATATTTCA AGGATCCCAG ACCATTCTGT CTCCATTTAT CATCACTGTT AAGTGAAGGA 
GGTGTGGTGG GGTGTGTCGA TGTAGTAATA CAAAGAATCT ATCCCATGCA GTGGATGGAA 
AAAATGGCAA ATGGACTGTA TGTGTTTCGC AATGATCGAG CAGAGGAAAG AGAAGCAGAA 
AAGCATTCAG CAAATCAACA AAAAAAGCTT GAAATGCTCT TTTCAAAAAT ACAAGCTGAA 
TTTGAACAGC GGGAAGTAAC ATGCAACAGA AGAAAGGGAC TCAGGCGGCG GTCATTAAAT 
GCACAACAAA TGCAGACCCT TCAGGATGGG GCTGAAATCT ATGAAGCCAT TCAGAACGAA 
TCTGACCCTG GGTATCTGGA GTCGTACTTA AGTGCTGAGC AGCTGAAAGC CCTCAATCAC 
CACAGGCAAC TTTTAAACGA TAAGAAGCAA GCACTGATCC AAGCTGAATT CAGAAAAGCC 
ATTGAATGCA GTGAGCAGGA TGCAAATGGC TGTACAAGGA GGGATGTCAC TCCTGTATGG 
AAATTACGCA TTGCAGATTA CAGAAATTAT GAAACAGATG CAGCATATAT ACTGAACATT 
TGGCGTCCGT TACCAGATGT GCTTTCTCTT CTGAAAGAAG GTTGTCGCTA TAAAATGTAT 
CATTTGGCAG CATCCACATC AAAAGGAAAG TCATTAGCAG CTGATTTGCA GTTAACAGCT 
ACAAAGAAAA CTCGCTTCCA GCAACTTCAG CCATCAGAGA GCATTTTGGA GCAGATTTAC 
AGCCCGAGAG AAGTGACGGA CTTCAGCAGG TTCCAGGAGC CTTTGTTTAG CGCCCCATAT 
GCAGAGGTTG ATCTGGTGGG ATTAATCATC TCCATATACA AAAAAACAGG TGCAGCTCCA 
GTGGTTTATA TATCAGATGA ATCTCACAAT ATTGTGGCGC TAAAATTCTG GACAGATCTA 
GGGCAGCTTG GGCTTGAGGA GATAACCAAG CCACGCACAT ATATCTCTGC CAGTAATCTT 
CGCTGGAGAT CAGACTGTAT CGAGGGGATT CCTACACTGT ACGTTGGAGA CTTAGCCAAC 
ATTTCTTCCA ATCCCAAAGA AAGCCATCTG CAGAGGGCAA TTCAGAAACT GAAACTTTCT 
GTACAGAATG TCCAAGATTT TTGGAATTCA TCACAAACCG CACTGATGAA AACCTTGCAG 
ATAAATAGCA CAGACACAAC GGAGTGCAGT AAGAACCCAA CGACACCAAC ATGGAAGTCA 
GATGTTTCTG CTAGAAGTGG TTACCTAACC CCCTTGCACC ATAGTGGTAA AAGGTTATTA 
AATTCTGTCC ATACATCAGA TCCTCAGACA GAAAATCCTG GATGTAGTAA AGAAATACAA 
CTAAAAACCT GCAAAAAGAG GAAAGCTCTG GACTTTCTCA ACCGCATTCC TTCACCTCCA 
CCTGTAACAC CAGTACGGCC CTTTGTATCC CCATCTTTGC AGAAGGCATT CCGACCCCCA 
CGGAGCTGTA GCGTCCAGAA GTTGGGCCCG GAAACCAAGG GTAATACTGA GAATGTCCAG 
GGAACAACCC CAGAATGCAC TAAAGACCTA GCAAAATTAG AGGGTGAATT TGTGGCTGAT 
GAAGAACTCG CCATGATCAA TACCCAAGCT TTGCTTTTAG GTCTGGAGGA AGAAAAAAAG 
AAAACCGAGC AGAAAACCAG CAGAACAGCA GGTAAAATGA CTGCCCATGA GTCTCCAATA 
GAAAATGCTT CCCCCGTTCC TGCCCAGGAA CAACAAACTG AGGAAGCATT GAACATTCCA 
GTCGGTAACT CAGAGAAATC TTACCTGTGC CTAAGAAAAA GGAAACGGAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031753097.1
CDS	281..9934
Translation	MAAPQLGKSVFYDLFSTHCSHSDLGPISLNWFEELTAEALPYKSRTCEDHECSLDDLHENNIKTPKLKSSIYSQLDSTPVIFKERLLSPLFASSLTELDKRQNATDNGNNVRLEKSNCTQMQHQASEVFSPSSRCLNESPAVIKEIFKTPLRNKYLHKTPQCDWKPDICSSLFCTPKLMKNQTGCIKESLGAEVDPEMSWSSSLATPPSPTVIIAHANDQPSGNKHAAIVQSLFPNCENMEANNLPPPETGTQNEDRQICGLEKPGSCSASKTVPEASAISSRAAWKKTVANTVKDEEVSRTVENALEGMEDVLSIFFASEKTPGLRKLKNSTQARRKVESIKSQKCQVSLFKAEEGESILVPLDEHHKCDHEMLMKPEQDYKMNAVNQKIAKETTPYEWSQLNICELDITQSQDSTLHAANLCCEDITVNKSDTCNITEAEKQEEVNEPSTDNVLSNKVGKESTLNVNCINADLNNMASSSNNSVHSLNLSQCEKMDSSEISNSEVGCITKLTTHPAKITTGELSSVDDCAKKPQERVTISTSFSTLKKQSKFMYSVNTVLTGHIASNTSIATRRSLNSHLSSDEPKPHIKENQNEPESNAKYYSNGLQKPVFLEKENDNKGISLCKTISDGQNTSEGARAPDYFEERVPETKDRCKATLSIREKVVATANCFARKQLETDYPEDASAQHEALQFHAKQYVSCSMLNSNVSNDMQIKPQVLTQACDFRSSAENLVEKTKNQLRRNEDQSFSSTGIGKKPASGEKLVGDCNEGSFLHVKQEISAAQALVGNGYETRDTPDVTSDHTRSVISNHGKSLWEEETALTELPVPEILSKTFKGFKTASNKKIHISDKNIVKGCDLFKEIEFGIGDAKPCNEENKEPLKKTPFDVPGYETNLKGFKTASNKEINVSENKFAKGRLLFKDIEEESGQTTAIMAKDNELFIKPASSNKPDIFKSTCIKGMNPSENLDSMPPKKGDVQQSAGLKHSDQIDVFGFDDPPAKGNVQTLGYFNMSASETDFKGFKTASNKDIIISESTLAKGKLIFEDIEDTRYSETGRTIDCKAKGGALSTSNLVMHNTMCKNEPSTSENTNDKAPKRAKVQTPMAENSHLKEPSSDKNAMDAANHQMTLKASAYSPKAASSALPRYKKKPISTTKTSFQLNEHLTESQQAEISELSSILENADSQFDFTQFRKVPSVTEKQNSTEGGSESQNLNNSDVWKDVDFNDSFAAGRDHSEGMEAIPSSPGIKELPSAGECCSKETHDSMPDFVTLAPKQGFLVKQNERLFAGFNLASGKQVNIDNDVLKKAAELFNDIDNDKELLSHAKEESRKSNIKHSSKLINNENCGKTEHTSEHLVCQSNVSLPFIKTLGKNATILVSGNEKSQNQELQLDCLKCESTDVKHTPQKETINDNSKCNESSLSELSMKGFQTASGRNIMMSESSIQKARNIFAEEHEDSFTLRCNIQNTIQIPQPVNEPTQFPYVNLGPKPTTTSGWQEKNILRRSTEKGFMPGFCTAGGKKVSVSDDSLAKAHKLFQEECTFSKEGKLDEVKQNKLMNSEPLSLLTCESVLKQSDGFIEDISTSRNALEIRPELYPEGMCSNRASNGSGNNSEFTAGEGISININQSSLLTTGNVLKNLPSESSGHDVYSVTEHLSTVVKVKRYNDSGHFVNQNLAECNDNHVLSTQKNTANISNRNEDCTSLAPLSFSTASGKSVTVSHDSLQKARLMLSEAANDVTVDTSKQEAAYITPAIRKTEAEKEQNTVDDSDRVNANTFSFSTASGKKVNISGNSLKQVRAVCLSSDPKETSAALFNVEKSVFNEDVKDVSLLQPNVTMPKAVSFSTASGKTVQLSDESLKKARVIFSEIDTCPLMQQQTNESTVEEIVIGGGMTKSKQMPLTTEKVETTRKNNGTFGFNTASGKQVSVSESALQKVKDIFQEFDDPDNYEQNKSLVRLPVSSKIKESTPGTKRLVQTAGSSYKNDNLQCKAGNLRTFQDKQAGKKSLTYSEAAISPIITTVPGNQLTPIQLVKVSTNTSALANKTKPDLYVATAQNTPQNDFEIEAAESARAFLEDDDLTDMGSAAHETYLYLNQTSNTRTGKRSRTEGPTPGEPPIKRRLLPEFDCTTDTRQQTGILKPLISTPDMMRDRRRFLYTLPLKPSTCNPESALRQVTCIPTQAHLHSKVKIFHQSLPIKSPDVASDSTSKSYSPTAAKETINCSSASKIPAKKFVPPFKKTVATLADNQSNSVQNGSSDGLIESIVYPKEDKVETICSSKDQFDDSDILQMTSNLRCSKDLQEMRIRKKLRQKIKPHPGSLYRLKMSHVKRISLQSAVAERCPTLYSREQLYRYGIVKNHIGVSSENALSFQFHCSNYFTKELLLSGNGVQLADGGWLIPTEQGNAGKEEIYRAFCDTPGVDPKLISAEWVHNHYRWIVWKLAAMEVRFPKTFACRCLTPERVLLQLKYRYDVEIDKSQRSAIKKIMERDDSPAKTLVLCIAKIISQGTRLPNACSNKTEPADSKESSAVIEVTDSWYGIKVLLDPCLTALLHKGRLFIGQKLIVHGAELIGSDDACSPLEAPESLMLKIAANSTRPVRWHTKLGYFKDPRPFCLHLSSLLSEGGVVGCVDVVIQRIYPMQWMEKMANGLYVFRNDRAEEREAEKHSANQQKKLEMLFSKIQAEFEQREVTCNRRKGLRRRSLNAQQMQTLQDGAEIYEAIQNESDPGYLESYLSAEQLKALNHHRQLLNDKKQALIQAEFRKAIECSEQDANGCTRRDVTPVWKLRIADYRNYETDAAYILNIWRPLPDVLSLLKEGCRYKMYHLAASTSKGKSLAADLQLTATKKTRFQQLQPSESILEQIYSPREVTDFSRFQEPLFSAPYAEVDLVGLIISIYKKTGAAPVVYISDESHNIVALKFWTDLGQLGLEEITKPRTYISASNLRWRSDCIEGIPTLYVGDLANISSNPKESHLQRAIQKLKLSVQNVQDFWNSSQTALMKTLQINSTDTTECSKNPTTPTWKSDVSARSGYLTPLHHSGKRLLNSVHTSDPQTENPGCSKEIQLKTCKKRKALDFLNRIPSPPPVTPVRPFVSPSLQKAFRPPRSCSVQKLGPETKGNTENVQGTTPECTKDLAKLEGEFVADEELAMINTQALLLGLEEEKKKTEQKTSRTAGKMTAHESPIENASPVPAQEQQTEEALNIPVGNSEKSYLCLRKRKRK

Target ORF information:

RefSeq Version	XM_031897237.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis BRCA2, DNA repair associated (brca2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031897237.1

ORF Insert Sequence:

ATGGCTGCAC CGCAACTTGG AAAATCTGTC TTCTATGATC TGTTTAGCAC GCATTGCTCT 
CACTCAGATT TAGGGCCTAT CAGTCTAAAC TGGTTTGAAG AGCTCACTGC TGAAGCCTTG 
CCTTACAAGT CAAGAACATG TGAAGATCAT GAATGCAGTT TGGATGATCT CCATGAAAAC 
AACATCAAAA CTCCTAAACT GAAGTCGTCA ATTTATAGCC AATTAGATTC CACTCCAGTC 
ATCTTTAAGG AGCGTTTACT ATCACCTCTT TTTGCTTCTT CTTTGACAGA GCTGGATAAA 
AGGCAGAATG CAACAGATAA TGGAAACAAT GTTAGACTTG AGAAAAGTAA CTGTACCCAA 
ATGCAGCATC AAGCAAGTGA AGTTTTCAGT CCCTCAAGTC GATGCCTCAA CGAAAGTCCT 
GCTGTTATCA AAGAAATATT CAAGACACCA TTACGAAATA AGTACCTACA TAAAACACCA 
CAGTGTGATT GGAAACCTGA CATTTGCAGC AGTCTGTTTT GTACTCCAAA GCTTATGAAG 
AACCAAACTG GATGTATTAA AGAAAGTCTA GGTGCAGAGG TGGATCCAGA GATGTCATGG 
TCTAGTTCAC TAGCTACACC TCCTTCCCCA ACTGTGATAA TTGCACATGC TAATGATCAG 
CCCAGTGGAA ATAAACATGC TGCAATTGTA CAGAGCCTTT TCCCAAATTG TGAGAATATG 
GAAGCGAATA ATTTGCCACC ACCTGAGACG GGCACACAAA ATGAAGACCG TCAAATATGT 
GGACTAGAAA AGCCAGGTTC ATGCAGTGCG AGCAAAACTG TACCAGAAGC GTCTGCCATT 
TCCTCAAGGG CAGCCTGGAA GAAAACTGTT GCAAATACAG TGAAGGACGA GGAGGTTTCC 
AGAACTGTGG AAAATGCTCT GGAAGGAATG GAAGATGTCC TCTCAATCTT TTTTGCAAGT 
GAAAAGACTC CTGGTCTCAG AAAGCTGAAA AATTCTACCC AAGCTAGGAG AAAAGTGGAA 
TCTATTAAGT CTCAGAAGTG CCAAGTTTCA TTATTTAAAG CAGAAGAAGG AGAAAGCATT 
TTGGTCCCTT TAGATGAGCA TCATAAATGT GATCATGAGA TGCTTATGAA ACCTGAACAA 
GACTACAAAA TGAATGCTGT TAACCAAAAA ATAGCCAAAG AAACTACTCC ATATGAATGG 
TCACAGCTAA ATATATGTGA GTTGGATATA ACTCAATCAC AGGATAGCAC TTTGCATGCA 
GCAAATCTAT GTTGTGAAGA TATCACTGTC AACAAAAGTG ATACATGTAA TATTACTGAA 
GCCGAAAAGC AGGAGGAAGT CAATGAGCCA AGCACCGACA ATGTACTGTC TAATAAAGTT 
GGCAAAGAAA GCACTTTAAA TGTGAATTGT ATTAATGCTG ATTTGAATAA CATGGCTTCA 
TCCTCAAATA ACTCTGTTCA CTCACTGAAT CTTTCTCAAT GTGAAAAAAT GGATTCTTCT 
GAAATTTCTA ACAGTGAAGT TGGTTGTATA ACAAAACTAA CAACCCACCC AGCAAAAATA 
ACAACTGGTG AATTAAGTTC AGTAGATGAC TGTGCTAAGA AACCCCAGGA AAGAGTAACT 
ATTAGTACTT CATTTTCAAC TCTGAAAAAG CAATCGAAGT TTATGTACAG TGTAAACACT 
GTGTTAACTG GTCATATTGC AAGCAATACC AGCATAGCCA CTAGGAGATC ATTGAACAGT 
CATTTAAGTT CTGATGAACC AAAACCTCAC ATTAAGGAAA ATCAAAATGA ACCAGAATCA 
AATGCTAAAT ATTATTCCAA CGGCCTGCAG AAACCAGTAT TCCTGGAAAA GGAGAATGAT 
AATAAGGGAA TTTCTTTATG TAAAACTATC TCAGACGGTC AAAACACTTC AGAAGGTGCC 
AGGGCTCCAG ACTATTTTGA GGAACGTGTT CCTGAAACAA AAGATAGATG CAAAGCTACA 
CTAAGCATCA GGGAAAAGGT TGTGGCTACA GCAAATTGTT TTGCAAGAAA ACAGCTTGAA 
ACAGATTATC CAGAGGATGC CTCAGCACAA CATGAGGCAC TTCAGTTCCA TGCTAAGCAA 
TATGTGTCAT GCTCAATGCT AAATTCTAAT GTGTCCAATG ACATGCAAAT AAAGCCTCAA 
GTGTTAACTC AGGCATGCGA TTTTAGGAGC AGCGCAGAAA ATCTTGTTGA AAAAACAAAA 
AATCAATTAA GGCGGAATGA AGATCAATCA TTTTCATCAA CTGGCATAGG GAAAAAGCCA 
GCTAGTGGTG AAAAATTAGT AGGTGACTGT AATGAGGGCT CATTCCTTCA TGTGAAGCAA 
GAAATAAGTG CTGCTCAGGC ATTGGTAGGT AATGGTTACG AAACCAGAGA CACTCCTGAT 
GTCACTAGTG ATCATACAAG GAGTGTTATT TCCAACCATG GAAAGTCTCT GTGGGAGGAG 
GAGACAGCAC TGACTGAATT ACCAGTCCCA GAAATTCTGA GTAAAACTTT CAAGGGCTTT 
AAAACGGCAT CTAATAAAAA AATACATATT TCAGATAAAA ATATTGTAAA AGGTTGTGAC 
TTGTTCAAAG AAATTGAGTT TGGCATTGGT GACGCTAAGC CCTGCAATGA AGAAAACAAA 
GAGCCCTTAA AGAAAACTCC CTTTGATGTT CCTGGGTATG AAACAAATTT AAAAGGATTC 
AAAACTGCAT CCAATAAAGA AATAAATGTA TCAGAAAACA AATTTGCTAA AGGTAGACTG 
CTGTTTAAAG ACATCGAAGA GGAAAGCGGT CAAACTACAG CTATAATGGC AAAAGATAAT 
GAGCTGTTCA TTAAGCCTGC ATCCAGCAAT AAGCCTGACA TATTTAAGAG TACATGTATA 
AAAGGAATGA ATCCATCTGA GAACCTAGAC AGTATGCCCC CCAAAAAAGG AGACGTGCAG 
CAGAGTGCAG GTTTAAAACA TTCAGATCAA ATTGACGTTT TTGGTTTCGA TGATCCTCCT 
GCAAAAGGAA ATGTTCAAAC ATTAGGCTAT TTTAATATGT CTGCTTCTGA AACAGACTTC 
AAAGGATTCA AAACCGCTTC CAATAAAGAC ATAATTATTT CAGAAAGCAC ACTTGCTAAA 
GGTAAACTTA TTTTTGAAGA TATTGAAGAT ACAAGATACA GTGAAACAGG AAGGACCATA 
GATTGTAAAG CAAAAGGTGG TGCGTTGTCC ACCAGTAACC TTGTAATGCA TAACACCATG 
TGTAAAAATG AACCAAGTAC ATCTGAGAAC ACAAATGATA AAGCACCAAA AAGAGCAAAA 
GTACAAACAC CTATGGCAGA AAATAGTCAT TTAAAAGAAC CTTCTTCAGA CAAAAATGCA 
ATGGATGCTG CAAATCATCA AATGACACTG AAAGCCTCCG CATACAGCCC AAAGGCTGCC 
TCTTCTGCAC TGCCCAGGTA CAAAAAGAAA CCAATCTCTA CCACTAAAAC CTCTTTCCAG 
TTGAATGAAC ATTTGACTGA AAGCCAGCAA GCTGAAATCT CTGAACTTTC CTCTATTCTA 
GAAAATGCAG ACAGTCAGTT TGATTTTACT CAGTTTAGGA AAGTGCCTTC TGTGACTGAG 
AAACAAAATT CAACTGAAGG TGGGAGCGAG TCCCAGAACC TTAACAACTC AGATGTCTGG 
AAGGACGTAG ATTTTAATGA TAGTTTTGCA GCAGGAAGGG ATCATTCTGA GGGAATGGAG 
GCAATTCCCT CCAGTCCTGG AATAAAAGAA TTGCCTTCTG CAGGAGAATG TTGCAGTAAA 
GAAACACATG ATTCTATGCC AGATTTTGTG ACCTTGGCAC CTAAACAAGG TTTTCTGGTT 
AAACAAAATG AAAGACTCTT TGCAGGGTTT AATCTTGCTA GTGGCAAGCA AGTTAATATA 
GACAATGATG TACTAAAAAA AGCTGCTGAA CTGTTCAATG ATATAGATAA TGATAAAGAA 
TTGCTCAGCC ACGCCAAAGA AGAAAGCAGA AAAAGCAATA TAAAACACTC TTCTAAATTA 
ATAAATAATG AAAATTGTGG AAAGACAGAA CACACATCTG AGCATTTGGT ATGTCAAAGT 
AATGTTTCAT TACCATTCAT AAAAACATTG GGCAAGAATG CTACAATACT AGTGAGTGGT 
AATGAAAAAA GCCAGAATCA AGAATTACAG CTGGATTGTT TAAAATGTGA AAGTACTGAT 
GTAAAGCATA CACCACAAAA GGAAACAATC AATGACAACT CAAAGTGTAA TGAATCCTCT 
CTTTCTGAGC TTTCCATGAA AGGTTTTCAG ACTGCCAGTG GGAGAAACAT TATGATGTCC 
GAGTCTTCAA TCCAGAAAGC CAGAAACATA TTTGCAGAGG AACATGAAGA CAGTTTTACA 
TTGCGCTGTA ATATTCAAAA TACAATACAA ATACCACAGC CTGTAAATGA ACCGACCCAA 
TTCCCATATG TTAATTTGGG GCCAAAACCC ACAACGACAT CAGGATGGCA AGAAAAAAAT 
ATTCTGAGAA GAAGTACAGA AAAAGGTTTC ATGCCGGGTT TTTGCACTGC CGGTGGTAAA 
AAGGTTTCTG TTTCCGACGA CTCTCTAGCT AAAGCACATA AACTTTTCCA GGAGGAATGT 
ACCTTTAGCA AGGAAGGGAA ATTGGATGAG GTGAAACAAA ATAAATTAAT GAACTCTGAG 
CCATTGTCTC TTCTCACTTG TGAGTCAGTC CTGAAACAGA GTGACGGATT CATTGAAGAT 
ATTTCAACTT CTAGAAATGC TTTGGAAATC AGACCAGAGC TTTATCCGGA GGGCATGTGT 
AGTAATAGGG CATCTAATGG AAGTGGAAAC AATAGTGAAT TTACAGCTGG GGAAGGCATA 
TCAATAAATA TCAACCAATC ATCGCTGCTT ACAACAGGCA ACGTTTTAAA AAATTTGCCG 
TCTGAAAGTA GTGGCCATGA TGTGTATTCA GTAACTGAGC ATCTAAGCAC TGTGGTTAAA 
GTCAAAAGAT ACAATGATTC AGGTCATTTT GTAAATCAAA ATTTAGCTGA ATGTAATGAT 
AACCATGTCC TTAGCACTCA AAAAAATACT GCAAATATAA GCAACAGAAA CGAGGACTGT 
ACATCCCTGG CACCTCTCTC TTTCAGCACA GCCAGTGGGA AATCCGTCAC AGTGTCTCAT 
GATTCTCTTC AAAAAGCAAG ACTGATGCTT TCTGAAGCAG CTAATGATGT TACAGTAGAT 
ACTAGTAAGC AAGAGGCGGC TTATATTACG CCAGCCATTC GAAAAACAGA AGCAGAAAAA 
GAGCAGAACA CTGTTGATGA TTCTGATAGA GTTAATGCAA ATACATTTTC ATTTTCTACT 
GCAAGCGGTA AAAAGGTCAA CATAAGCGGA AATTCTTTAA AGCAGGTCAG AGCTGTCTGC 
CTATCCTCTG ACCCAAAAGA AACATCAGCG GCTCTGTTTA ATGTTGAAAA AAGTGTTTTT 
AATGAAGATG TTAAGGATGT ATCTTTGCTT CAGCCCAATG TTACCATGCC GAAGGCTGTG 
TCGTTTAGTA CTGCAAGTGG CAAGACGGTG CAGTTGTCAG ACGAGTCTTT GAAGAAAGCT 
AGGGTGATTT TTTCTGAAAT AGATACTTGT CCGTTGATGC AACAGCAGAC TAATGAAAGC 
ACTGTTGAAG AGATAGTAAT AGGAGGAGGA ATGACAAAAT CAAAACAGAT GCCATTAACA 
ACAGAAAAAG TCGAAACAAC TAGAAAAAAT AATGGTACTT TTGGCTTTAA TACCGCAAGC 
GGAAAGCAGG TCTCTGTGTC TGAAAGTGCG CTGCAGAAAG TCAAAGATAT CTTTCAAGAA 
TTTGATGATC CCGACAATTA TGAACAAAAT AAATCACTCG TAAGACTGCC TGTTTCATCA 
AAAATAAAAG AATCAACTCC AGGAACAAAA AGACTGGTGC AAACTGCAGG GTCCTCTTAT 
AAAAATGATA ATTTGCAATG TAAAGCAGGG AATCTTCGTA CTTTTCAGGA TAAGCAAGCT 
GGCAAGAAGT CACTTACATA TTCAGAAGCA GCCATAAGCC CCATTATAAC AACTGTCCCT 
GGAAATCAAT TAACGCCCAT CCAGTTGGTA AAAGTCTCAA CCAACACATC AGCATTGGCT 
AATAAAACCA AACCCGATTT ATATGTCGCA ACAGCCCAGA ACACACCACA AAATGATTTT 
GAAATAGAAG CAGCAGAAAG TGCTAGAGCT TTCTTGGAAG ATGATGACCT GACAGATATG 
GGATCAGCTG CCCATGAAAC ATACTTGTAT CTCAACCAGA CCTCAAATAC AAGAACTGGG 
AAGAGATCAA GAACAGAAGG GCCGACACCT GGAGAGCCAC CAATCAAAAG ACGGCTTTTA 
CCAGAATTTG ATTGCACAAC AGACACTCGG CAACAAACGG GGATTTTAAA GCCTTTGATC 
AGCACTCCAG ACATGATGCG AGACAGGCGA AGGTTTTTGT ACACCCTTCC CTTGAAGCCA 
TCAACTTGTA ATCCTGAAAG TGCTTTACGT CAAGTCACGT GCATTCCTAC ACAAGCCCAT 
CTCCATTCTA AAGTCAAAAT CTTCCACCAG TCTCTACCCA TCAAGTCACC AGATGTTGCA 
TCTGATTCAA CTTCTAAATC GTATAGCCCC ACTGCTGCAA AAGAGACCAT AAACTGCTCA 
TCTGCATCTA AAATACCTGC AAAGAAATTT GTGCCTCCTT TTAAAAAGAC TGTGGCGACC 
TTGGCAGACA ACCAGTCAAA CAGTGTACAA AATGGCAGTT CGGATGGGCT TATCGAGAGC 
ATTGTTTATC CAAAAGAAGA CAAAGTTGAA ACCATATGTT CTTCAAAAGA TCAATTTGAT 
GACTCAGATA TTTTACAAAT GACTTCAAAT CTTCGATGTA GCAAAGACCT GCAGGAAATG 
AGAATTCGGA AAAAGCTAAG GCAAAAGATT AAACCGCATC CAGGCTCACT GTATCGTCTG 
AAAATGTCAC ATGTGAAGAG AATTTCACTT CAATCTGCAG TGGCAGAAAG GTGTCCCACT 
CTGTATTCAA GGGAACAGTT ATACAGATAT GGCATTGTGA AGAACCATAT TGGAGTCAGC 
AGCGAGAACG CATTGTCCTT CCAGTTCCAC TGTTCAAATT ATTTCACAAA AGAACTTCTT 
CTTTCTGGAA ATGGTGTGCA GTTGGCAGAT GGAGGTTGGC TTATTCCCAC TGAACAAGGC 
AACGCAGGCA AAGAAGAGAT ATACAGAGCG TTTTGTGACA CTCCTGGTGT GGATCCAAAA 
TTAATTTCAG CTGAATGGGT TCATAACCAC TACCGCTGGA TTGTATGGAA ACTTGCTGCA 
ATGGAAGTCA GGTTCCCAAA GACATTTGCT TGCAGATGCC TTACACCAGA ACGTGTGCTT 
TTACAGTTAA AGTACAGATA TGATGTGGAA ATTGATAAGA GTCAGAGATC CGCCATCAAA 
AAGATCATGG AGAGAGATGA CTCCCCAGCT AAAACGCTGG TACTGTGCAT TGCCAAGATC 
ATTTCCCAGG GCACTCGCCT GCCCAATGCA TGTAGTAATA AAACCGAACC CGCTGACTCT 
AAGGAAAGTT CTGCAGTAAT AGAGGTGACA GATAGTTGGT ATGGTATCAA GGTTCTGCTT 
GATCCGTGTC TCACTGCTCT CTTACACAAA GGAAGGCTTT TTATTGGTCA GAAGCTAATT 
GTTCATGGAG CGGAACTTAT TGGCTCAGAT GATGCCTGCT CTCCCCTTGA AGCGCCAGAA 
TCTCTTATGT TGAAGATTGC AGCTAACAGC ACTCGGCCTG TTCGATGGCA TACCAAACTT 
GGATATTTCA AGGATCCCAG ACCATTCTGT CTCCATTTAT CATCACTGTT AAGTGAAGGA 
GGTGTGGTGG GGTGTGTCGA TGTAGTAATA CAAAGAATCT ATCCCATGCA GTGGATGGAA 
AAAATGGCAA ATGGACTGTA TGTGTTTCGC AATGATCGAG CAGAGGAAAG AGAAGCAGAA 
AAGCATTCAG CAAATCAACA AAAAAAGCTT GAAATGCTCT TTTCAAAAAT ACAAGCTGAA 
TTTGAACAGC GGGAAGTAAC ATGCAACAGA AGAAAGGGAC TCAGGCGGCG GTCATTAAAT 
GCACAACAAA TGCAGACCCT TCAGGATGGG GCTGAAATCT ATGAAGCCAT TCAGAACGAA 
TCTGACCCTG GGTATCTGGA GTCGTACTTA AGTGCTGAGC AGCTGAAAGC CCTCAATCAC 
CACAGGCAAC TTTTAAACGA TAAGAAGCAA GCACTGATCC AAGCTGAATT CAGAAAAGCC 
ATTGAATGCA GTGAGCAGGA TGCAAATGGC TGTACAAGGA GGGATGTCAC TCCTGTATGG 
AAATTACGCA TTGCAGATTA CAGAAATTAT GAAACAGATG CAGCATATAT ACTGAACATT 
TGGCGTCCGT TACCAGATGT GCTTTCTCTT CTGAAAGAAG GTTGTCGCTA TAAAATGTAT 
CATTTGGCAG CATCCACATC AAAAGGAAAG TCATTAGCAG CTGATTTGCA GTTAACAGCT 
ACAAAGAAAA CTCGCTTCCA GCAACTTCAG CCATCAGAGA GCATTTTGGA GCAGATTTAC 
AGCCCGAGAG AAGTGACGGA CTTCAGCAGG TTCCAGGAGC CTTTGTTTAG CGCCCCATAT 
GCAGAGGTTG ATCTGGTGGG ATTAATCATC TCCATATACA AAAAAACAGG TGCAGCTCCA 
GTGGTTTATA TATCAGATGA ATCTCACAAT ATTGTGGCGC TAAAATTCTG GACAGATCTA 
GGGCAGCTTG GGCTTGAGGA GATAACCAAG CCACGCACAT ATATCTCTGC CAGTAATCTT 
CGCTGGAGAT CAGACTGTAT CGAGGGGATT CCTACACTGT ACGTTGGAGA CTTAGCCAAC 
ATTTCTTCCA ATCCCAAAGA AAGCCATCTG CAGAGGGCAA TTCAGAAACT GAAACTTTCT 
GTACAGAATG TCCAAGATTT TTGGAATTCA TCACAAACCG CACTGATGAA AACCTTGCAG 
ATAAATAGCA CAGACACAAC GGAGTGCAGT AAGAACCCAA CGACACCAAC ATGGAAGTCA 
GATGTTTCTG CTAGAAGTGG TTACCTAACC CCCTTGCACC ATAGTGGTAA AAGGTTATTA 
AATTCTGTCC ATACATCAGA TCCTCAGACA GAAAATCCTG GATGTAGTAA AGAAATACAA 
CTAAAAACCT GCAAAAAGAG GAAAGCTCTG GACTTTCTCA ACCGCATTCC TTCACCTCCA 
CCTGTAACAC CAGTACGGCC CTTTGTATCC CCATCTTTGC AGAAGGCATT CCGACCCCCA 
CGGAGCTGTA GCGTCCAGAA GTTGGGCCCG GAAACCAAGG GTAATACTGA GAATGTCCAG 
GGAACAACCC CAGAATGCAC TAAAGACCTA GCAAAATTAG AGGGTGAATT TGTGGCTGAT 
GAAGAACTCG CCATGATCAA TACCCAAGCT TTGCTTTTAG GTCTGGAGGA AGAAAAAAAG 
AAAACCGAGC AGAAAACCAG CAGAACAGCA GGTAAAATGA CTGCCCATGA GTCTCCAATA 
GAAAATGCTT CCCCCGTTCC TGCCCAGGAA CAACAAACTG AGGAAGCATT GAACATTCCA 
GTCGGTAACT CAGAGAAATC TTACCTGTGC CTAAGAAAAA GGAAACGGAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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1-732-210-0262

brca2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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