rims2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

  • Gene

  • Clones

  • gRNAs

The following rims2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rims2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa11430 XM_004915103.3
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 2 (rims2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OXa45264 XM_018095168.1
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 2 (rims2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OXa59919 XM_031903577.1
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 2 (rims2), transcript variant X31, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa59926 XM_031903581.1
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 2 (rims2), transcript variant X38, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa59928 XM_031903583.1
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 2 (rims2), transcript variant X40, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa59929 XM_031903584.1
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 2 (rims2), transcript variant X41, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa59930 XM_031903585.1
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 2 (rims2), transcript variant X42, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa59897 XM_031903556.1
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 2 (rims2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa59898 XM_031903557.1
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 2 (rims2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa59899 XM_031903558.1
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 2 (rims2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa59900 XM_031903559.1
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 2 (rims2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa59901 XM_031903560.1
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 2 (rims2), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa59904 XM_031903563.1
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 2 (rims2), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa59909 XM_031903568.1
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 2 (rims2), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa59910 XM_031903569.1
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 2 (rims2), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa59913 XM_031903571.1
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 2 (rims2), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa59915 XM_031903573.1
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 2 (rims2), transcript variant X27, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa59917 XM_031903575.1
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 2 (rims2), transcript variant X29, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa11423 XM_004915096.3
Latest version!
Xenopus tropicalis regulating synaptic membrane exocytosis 2 (rims2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa11430
    Clone ID Related Accession (Same CDS sequence) XM_004915103.3
    Accession Version XM_004915103.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 855bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004915103.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGGCAGGG AGCAGCGCCA GCAGCAGCAG CAAAGGGCCC CCCGCATCCA GCGATCGCAG 
    AGCCGCAGCA GCCTGTCCGC TTCCTTCGAG GCTCTGGCCG GCTACTTCCC CTGTATGAAC
    TCCCTGGAGG AGGATGAAGA AGTCGGAGGG AAGAAGCTGA AGAGCACCAT CCAGAGAAGT
    ACGGAAACAG GCCTGGCCGT GGAAATGAGA AACTGGATGA GCCGCCAAGC AAGCAGGGAG
    TCCACAGATG GGAGTATGAA CAGCTACAGC TCAGAGGGGA ATCTGATATT CCCTGGGGTC
    CGACTGGGTT CCGACAGCCA GTTCAGTGAC TTTTTGGATG GTCTTGGTCC TGCCCAGCTG
    GTTGGTCGCC AGACTTTGGC CACGCCCTCT ATGGGAGATA TTCAGGTTGG AATGGCTGAC
    AAAAAAGGCC AGTTGGAGGT GGAGGTGATA CGAGCCCGGG GTCTTGTTGT AAAACCAGGC
    TCAAAGACAC TGCCAGCACC CTATGTTAAG GTGTATCTCT TAGAAAATGG AGTGTGCATA
    GCCAAAAAGA AAACAAAAGT AGCAAGAAAA ACGCTGGAGC CTCTATACCA GCAAGTGTTA
    GCGTTTGAGG AATCACCACA AGGGAAGGTC CTACAGATCA TTGTTTGGGG TGACTATGGA
    CGCATGGACC ACAAGTCCTT CATGGGGGTG GCTCAGATAC TTTTAGATGA ACTGGACCTA
    TCCAGCATGG TGATTGGATG GTATAAACTT TTCCCCCCTT CATCTTTAGT AGACCCAACG
    TTGGCTCCAC TCACTAGGAG AGCTTCCCAA TCATCTCTTG AAAGCTCCAC GGGACCTTCT
    TATGCTCGTT CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004915160.1
    CDS465..1319
    Translation

    Target ORF information:

    RefSeq Version XM_004915103.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 2 (rims2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004915103.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGGCAGGG AGCAGCGCCA GCAGCAGCAG CAAAGGGCCC CCCGCATCCA GCGATCGCAG 
    AGCCGCAGCA GCCTGTCCGC TTCCTTCGAG GCTCTGGCCG GCTACTTCCC CTGTATGAAC
    TCCCTGGAGG AGGATGAAGA AGTCGGAGGG AAGAAGCTGA AGAGCACCAT CCAGAGAAGT
    ACGGAAACAG GCCTGGCCGT GGAAATGAGA AACTGGATGA GCCGCCAAGC AAGCAGGGAG
    TCCACAGATG GGAGTATGAA CAGCTACAGC TCAGAGGGGA ATCTGATATT CCCTGGGGTC
    CGACTGGGTT CCGACAGCCA GTTCAGTGAC TTTTTGGATG GTCTTGGTCC TGCCCAGCTG
    GTTGGTCGCC AGACTTTGGC CACGCCCTCT ATGGGAGATA TTCAGGTTGG AATGGCTGAC
    AAAAAAGGCC AGTTGGAGGT GGAGGTGATA CGAGCCCGGG GTCTTGTTGT AAAACCAGGC
    TCAAAGACAC TGCCAGCACC CTATGTTAAG GTGTATCTCT TAGAAAATGG AGTGTGCATA
    GCCAAAAAGA AAACAAAAGT AGCAAGAAAA ACGCTGGAGC CTCTATACCA GCAAGTGTTA
    GCGTTTGAGG AATCACCACA AGGGAAGGTC CTACAGATCA TTGTTTGGGG TGACTATGGA
    CGCATGGACC ACAAGTCCTT CATGGGGGTG GCTCAGATAC TTTTAGATGA ACTGGACCTA
    TCCAGCATGG TGATTGGATG GTATAAACTT TTCCCCCCTT CATCTTTAGT AGACCCAACG
    TTGGCTCCAC TCACTAGGAG AGCTTCCCAA TCATCTCTTG AAAGCTCCAC GGGACCTTCT
    TATGCTCGTT CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa45264
    Clone ID Related Accession (Same CDS sequence) XM_018095168.1
    Accession Version XM_018095168.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2265bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGTAAGC CTAGGTTGGC ATGTACATAT TGCGTTCACC ATCTCTGCCC ATTTTTTGGC 
    AGCAAGGATC ACCCTATTTA TCCTATCTTT TGTGAAGATG CAATCCGCTT GCTGCGCTCC
    CGTAAGATGT GCCGCACTTA TTCCGAGGGT GCATACAATG ATCATGAGAG GAGAACTAGA
    CAGGAGAGAC GAGTGCCTAT TCATTGTGAT GAAACTACCC CTTCTCCTGA AAGGTTATAC
    AATGGTGCCA GCTCCTGGGC AGACCATGTA GTCAATGGCT CAGCAGAACC TCAGGGGAAT
    TGTGAAGCGG CAGATAGACA GCCATATCAA AGATCCAGGT CCACTGAACA ACGGCCTGTG
    CTGGAGCGGA CCAGCTCCCG CTCCCGATCT ACTGAGCGTC CTGACTCAAA CCTCATGAGG
    TCGATGCCTT CATTAATGAC GGGAAGATCT GCCCCTCCTT CACCCGCCTT ATCGAGGGCA
    CACCCTCCTC GTACCGGCTC TGTTCAGACA AGTCCTTCAG GCACCCCGTT AGCAGGGCGG
    AGGGGGCGCC AGCTCCCGCA ACTTCCCCCA AAAGGCACGT TAGAAAGAAA AGTTACTTGG
    GAAGACCAGC AAAGAAAGGT GAATGGTTCA ATAACTGATG ACAAACATTT GCCAAGAAAG
    AAACAGCATT CTGAGCCAGA AGATATGGAC TCAACGCGGA GGCGAAACTC AGAGGAAAAG
    GCTGCAGAAC AAGAGAATGG GAACCCAGGT GCTATGGACG TAGAAGAAAG AACGCGACAG
    CTGAAAGTGA AGATCAACAA ATACAAGCAG GTGGGAGGAT CAGATTCTCG GCTAGAGAAG
    GATTATCATC CAAAGTATCG GACGGGGCGG GATCCGCAGA GGGGCTCAGA CAATATGTCC
    AACAAGTCAT CAGACAGCGA TGTTAGTGAT GTGTCTGCCG TATCACGGAC CAGCAGCGCC
    TCTCGTTTTA GTAGTACGAG TTATATGTCT GTGCAGTCGG AAAGGCCTCG AGGGAACATG
    AAAATAGGTA GAAGGACAAG GGAAGTAGAG GAGAGAATAG AGAGGGCTGA GGGAGGGCAG
    CATCACAAAG AAATAGGTTT TCTGGAGGAA GAGGAGGACG TTAGGCAGGA AGTAGATAGT
    GAGAAGGAAA CAGAAGTGCA GCATATAATT GACAACTGCA TGAAGGGGCC AAGCAAAGAA
    AGTGTAGAGG AAGAGAGTCA ACAGAATGAG AAAGATGAGG AAAGCCATGA TGTTGAGGAA
    GCCCATAATG AGAATCTGCC AAGGAGGTCC TCAGAAAATG ATATAAGTGC ATTGGCATCC
    AAAATGCAAA GCAGGCAAAT GGGCGCCTCA GGGAAGAACA TGACCAAAAG TACCAGCATC
    GGAGGGGATA TGTACACCCT GGAGAAGAAC GACGGAAGCC AGTCTGACAC AGCCGTGGGC
    ACTGTGGGTG CGGGTGGAAA GAAGAGACGT TCAAGCATAG GCGCCAAAAT GGTGGCCATT
    GTGGGCTTGT CACGAAAAAG CCGAAGTACC TCACAGCTCA GCCAAACAGA AGTCGGAGGG
    AAGAAGCTGA AGAGCACCAT CCAGAGAAGT ACGGAAACAG GCCTGGCCGT GGAAATGAGA
    AACTGGATGA GCCGCCAAGC AAGCAGGGAG TCCACAGATG GGAGTATGAA CAGCTACAGC
    TCAGAGGGGA ATCTGATATT CCCTGGGGTC CGACTGGGTT CCGACAGCCA GTTCAGTGAC
    TTTTTGGATG GTCTTGGTCC TGCCCAGCTG GTTGGTCGCC AGACTTTGGC CACGCCCTCT
    ATGGGAGATA TTCAGGTTGG AATGGCTGAC AAAAAAGGCC AGTTGGAGGT GGAGGTGATA
    CGAGCCCGGG GTCTTGTTGT AAAACCAGGC TCAAAGACAC TGCCAGCACC CTATGTTAAG
    GTGTATCTCT TAGAAAATGG AGTGTGCATA GCCAAAAAGA AAACAAAAGT AGCAAGAAAA
    ACGCTGGAGC CTCTATACCA GCAAGTGTTA GCGTTTGAGG AATCACCACA AGGGAAGGTC
    CTACAGATCA TTGTTTGGGG TGACTATGGA CGCATGGACC ACAAGTCCTT CATGGGGGTG
    GCTCAGATAC TTTTAGATGA ACTGGACCTA TCCAGCATGG TGATTGGATG GTATAAACTT
    TTCCCCCCTT CATCTTTAGT AGACCCAACG TTGGCTCCAC TCACTAGGAG AGCTTCCCAA
    TCATCTCTTG AAAGCTCCAC GGGACCTTCT TATGCTCGTT CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017950657.1
    CDS524..2788
    Translation

    Target ORF information:

    RefSeq Version XM_018095168.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 2 (rims2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018095168.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGTAAGC CTAGGTTGGC ATGTACATAT TGCGTTCACC ATCTCTGCCC ATTTTTTGGC 
    AGCAAGGATC ACCCTATTTA TCCTATCTTT TGTGAAGATG CAATCCGCTT GCTGCGCTCC
    CGTAAGATGT GCCGCACTTA TTCCGAGGGT GCATACAATG ATCATGAGAG GAGAACTAGA
    CAGGAGAGAC GAGTGCCTAT TCATTGTGAT GAAACTACCC CTTCTCCTGA AAGGTTATAC
    AATGGTGCCA GCTCCTGGGC AGACCATGTA GTCAATGGCT CAGCAGAACC TCAGGGGAAT
    TGTGAAGCGG CAGATAGACA GCCATATCAA AGATCCAGGT CCACTGAACA ACGGCCTGTG
    CTGGAGCGGA CCAGCTCCCG CTCCCGATCT ACTGAGCGTC CTGACTCAAA CCTCATGAGG
    TCGATGCCTT CATTAATGAC GGGAAGATCT GCCCCTCCTT CACCCGCCTT ATCGAGGGCA
    CACCCTCCTC GTACCGGCTC TGTTCAGACA AGTCCTTCAG GCACCCCGTT AGCAGGGCGG
    AGGGGGCGCC AGCTCCCGCA ACTTCCCCCA AAAGGCACGT TAGAAAGAAA AGTTACTTGG
    GAAGACCAGC AAAGAAAGGT GAATGGTTCA ATAACTGATG ACAAACATTT GCCAAGAAAG
    AAACAGCATT CTGAGCCAGA AGATATGGAC TCAACGCGGA GGCGAAACTC AGAGGAAAAG
    GCTGCAGAAC AAGAGAATGG GAACCCAGGT GCTATGGACG TAGAAGAAAG AACGCGACAG
    CTGAAAGTGA AGATCAACAA ATACAAGCAG GTGGGAGGAT CAGATTCTCG GCTAGAGAAG
    GATTATCATC CAAAGTATCG GACGGGGCGG GATCCGCAGA GGGGCTCAGA CAATATGTCC
    AACAAGTCAT CAGACAGCGA TGTTAGTGAT GTGTCTGCCG TATCACGGAC CAGCAGCGCC
    TCTCGTTTTA GTAGTACGAG TTATATGTCT GTGCAGTCGG AAAGGCCTCG AGGGAACATG
    AAAATAGGTA GAAGGACAAG GGAAGTAGAG GAGAGAATAG AGAGGGCTGA GGGAGGGCAG
    CATCACAAAG AAATAGGTTT TCTGGAGGAA GAGGAGGACG TTAGGCAGGA AGTAGATAGT
    GAGAAGGAAA CAGAAGTGCA GCATATAATT GACAACTGCA TGAAGGGGCC AAGCAAAGAA
    AGTGTAGAGG AAGAGAGTCA ACAGAATGAG AAAGATGAGG AAAGCCATGA TGTTGAGGAA
    GCCCATAATG AGAATCTGCC AAGGAGGTCC TCAGAAAATG ATATAAGTGC ATTGGCATCC
    AAAATGCAAA GCAGGCAAAT GGGCGCCTCA GGGAAGAACA TGACCAAAAG TACCAGCATC
    GGAGGGGATA TGTACACCCT GGAGAAGAAC GACGGAAGCC AGTCTGACAC AGCCGTGGGC
    ACTGTGGGTG CGGGTGGAAA GAAGAGACGT TCAAGCATAG GCGCCAAAAT GGTGGCCATT
    GTGGGCTTGT CACGAAAAAG CCGAAGTACC TCACAGCTCA GCCAAACAGA AGTCGGAGGG
    AAGAAGCTGA AGAGCACCAT CCAGAGAAGT ACGGAAACAG GCCTGGCCGT GGAAATGAGA
    AACTGGATGA GCCGCCAAGC AAGCAGGGAG TCCACAGATG GGAGTATGAA CAGCTACAGC
    TCAGAGGGGA ATCTGATATT CCCTGGGGTC CGACTGGGTT CCGACAGCCA GTTCAGTGAC
    TTTTTGGATG GTCTTGGTCC TGCCCAGCTG GTTGGTCGCC AGACTTTGGC CACGCCCTCT
    ATGGGAGATA TTCAGGTTGG AATGGCTGAC AAAAAAGGCC AGTTGGAGGT GGAGGTGATA
    CGAGCCCGGG GTCTTGTTGT AAAACCAGGC TCAAAGACAC TGCCAGCACC CTATGTTAAG
    GTGTATCTCT TAGAAAATGG AGTGTGCATA GCCAAAAAGA AAACAAAAGT AGCAAGAAAA
    ACGCTGGAGC CTCTATACCA GCAAGTGTTA GCGTTTGAGG AATCACCACA AGGGAAGGTC
    CTACAGATCA TTGTTTGGGG TGACTATGGA CGCATGGACC ACAAGTCCTT CATGGGGGTG
    GCTCAGATAC TTTTAGATGA ACTGGACCTA TCCAGCATGG TGATTGGATG GTATAAACTT
    TTCCCCCCTT CATCTTTAGT AGACCCAACG TTGGCTCCAC TCACTAGGAG AGCTTCCCAA
    TCATCTCTTG AAAGCTCCAC GGGACCTTCT TATGCTCGTT CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa59919
    Clone ID Related Accession (Same CDS sequence) XM_031903577.1
    Accession Version XM_031903577.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3966bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 2 isoform X31
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCAGTTCG AGACGCTGCG CCAGTTTTGC AACACGGTCC TGTCGCATTT CCACGAAGCT 
    TTCCCCACTC CGCCGAACAT ACTGCAGAAT GATCTGTTCG GGCAGCCAGG GAACAGCCCC
    GGGAAAAGAA GCCCTTCAAT ACCTAGAGAC CAGAATCGGA GATATGATAC AAGGGACGAT
    ATAGGTGAAC ATTCACAGCA TGCTACATCA GACAGCGCCA TGCCTAGATC ACCATCAGAC
    TACTCGGACA GGCGATTGCA ACGAGGGTCT CAGCTCTATG ATGAGCCTGA GCACGGTGAC
    TACAGAAGAA GTAGGCGGCG CTCCAATGAG TATTCGGTAG ATGAGGAGGA TATCCAAGAC
    AGGGAGGAAT ATGAAAGGCA GAGAAGAGAG GAGGAGTACC AGGCAAGGTA CCGGAGTGAC
    CCCAACTTGG CCAGGTACCC TGTTAAGCCA CAACCGTATG AAGAGCAAAT GCGCATCCAT
    GCAGAGGTTT CAAGGGCTCG ACAACGAAGG CACAGCGATG TGTCTTTAGC TCAGACAGAA
    TTTGAGGAGT CTCGGATGTC AACGCTGAGG ATGGAGAGAC CTTCAAGGCA AAGATCTGCG
    TCAGAGCGTA GAGCAGCGAT GGAAAGCCAG CGTTCATATT CAATAGAAAG AACTAGAGAT
    GTGCAAGGGC CAGGTCCTGG TCGGCAAAGG ACCTCAAATC ATAGTCCCCC AACACCAAGA
    AGGAGTCCCA TTCCCAATGA TAGGCCAGAT ATGAGGCGCA CAGATTCTTT ACGGAAACAG
    CACCACTTGG ACCCAAGTTC TGCCGTAAGG AAAACCAAAA GGGAAAAAAT GGAAACTATG
    TTAAGGAATG ATTCTCTTAG CTCAGATCAG TCTGAATCTG TCCGGCCCCC ACCACCAAAG
    CCTCATAAAA CAAAAAAGGG AGGTAAAATG CGTCAGGTTT CCTTGAGCAG TTCAGAAGAA
    GAGTTGGCGT CTACGCCGGA GTACACGAGC TGCGATGATG TGGAGATTGA AAGTGAAAGT
    GTTAGTGAGA AAGGTGACAT GGACTACAAC TGGGTGGAGC ATTCGTCTTG GCACAGCAGT
    GAGGCCTCCC CAATGTCTTT GCACCCTGTC ACGTGGCAAC CTTCCAAAGA TGGAGACCGC
    TTAATTGGTC GCATATTATT AAACAAGCGG CTCAAAGATG GGAGCGTCCC GCGAGATTCT
    GGAGCCATGC TGGGGCTGAA GGTCGTTGGA GGGAAAATGA CAGAATCAGG TCGACTTTGT
    GCGTTTATCA CCAAAGTGAA GAAGGGAAGC CTGGCAGATA CAGTAGGCCA TCTTCGACCA
    GGTGATGAGG TTTTAGAATG GAGTGGGCGG ATTTTGCAAG GAGCGACGTT CGAAGAAGTT
    TACAATATTA TACTGGAGTC CAAACCCGAG GCACAAGTAG AATTATTGGT CTCCAGACCC
    ATAGGAGATA TACCCCGGAT TCCTGACAGC ACACACGCAC AACTGGAATC CAGTTCTAGC
    TCATTTGAAT CCCAGAAAAT GGATCGCCCT TCTATTTCAG TTACTTCCCC CATGAGTCCT
    GGAATGTTAC GGGATGTCCC CCAGTTTCTT TCTGGACAAC TTTCAATTAA ACTCTGGTAT
    GACAAGGTTG GCCACCAGTT AATTGTCACG ATATTAGGAG CAAAGGATCT GCCATCGCGG
    GAAGATGGGA GGCCGAGAAA TCCTTACGTA AAAATTTACT TTCTTCCAGA TCGAAGTGAT
    AAGAACAAAA GGAGAACAAA GACTGTGAAG AAAACCGTAG AGCCCAAATG GAACCAAACA
    TTCATCTATT CTCCGGTTCA TCGGAGGGAA TTTCGGGAAC GAATGTTGGA AATAACACTT
    TGGGATCAAG CTCGGGTTCG AGAAGAAGAG AGTGAATTTT TAGGAGAGAT TCTAATTGAA
    TTAGAAACTG CACTATTAGA TGATGAGCCA CATTGGTACA AGCTACAGAC GCATGATGTG
    TCTTCATTGC CACTTCCACA CCCATCCCCG TTCATGCAAA GAAGGCAGCT GCACGGCGAG
    AGCCCAACCC GGAGGTTGCA AAGATCCAAA AGGATAAGCG ACAGCGAAGT GTCTGACTAC
    GACTGTGATG ATGGCGTTGG TGTTGTTTCA GACTTTCGGC ATAATGGGCG TGACCTGCAG
    AGTTCAACGT TATCTGTTCC GGAACAAGTA ATGTCGTCCA ATCACTGTTC CCGGACTGGG
    CCCCCGCATG TAGACAGCAT AGGAAGAACG AGGTCATGGT CTCCCAGTGT CCCTCCTCCG
    CAAAGTAGGA ATTTGGAACA GGGACCGAGA GGGACAAGAT CTGGCCCTGC ACATTACAAT
    ACCATGAGCA GAGTGGAAAG GCATCGAGTA TTAGATAACC ACTACTCCCC AGACAGAGAC
    AGGAATTGTG AAGCGGCAGA TAGACAGCCA TATCAAAGAT CCAGGTCCAC TGAACAACGG
    CCTGTGCTGG AGCGGACCAG CTCCCGCTCC CGATCTACTG AGCGTCCTGA CTCAAACCTC
    ATGAGGTCGA TGCCTTCATT AATGACGGGA AGATCTGCCC CTCCTTCACC CGCCTTATCG
    AGGGCACACC CTCCTCGTAC CGGCTCTGTT CAGACAAGTC CTTCAGGCAC CCCGTTAGCA
    GGGCGGAGGG GGCGCCAGCT CCCGCAACTT CCCCCAAAAG GCACGTTAGA AAGAAAAGAT
    ATGGACTCAA CGCGGAGGCG AAACTCAGGT GCTATGGACG TAGAAGAAAG GACGCGACAG
    CTGAAAGTGA AGATCAACAA ATACAAGCAG GTGGGAGGAT CAGATTCTCG GCTAGAGAAG
    GATTATCATC CAAAGTATCG GACGGGGCGG GATCCGCAGA GGGGCTCAGA CAATATGTCC
    AACAAGTCAT CAGACAGCGA TGTTAGTGAT GTGTCTGCCG TATCACGGAC CAGCAGCGCC
    TCTCGTTTTA GTAGTACGAG TTATATGTCT GTGCAGTCGG AAAGGCCTCG AGGGAACATG
    AAAATAGGTG CATTGGCATC CAAAATGCAA AGCAGGCAAA TGGGCGCCTC AGGGAAGAAC
    ATGACCAAAA GTACCAGCAT CGGAGGGGAT ATGTACACCC TGGAGAAGAA CGACGGAAGC
    CAGTCTGACA CAGCCGTGGG CACTGTGGGT GCGGGTGGAA AGAAGAGACG TTCAAGCATA
    GGCGCCAAAA TGGTGGCCAT TGTGGGCTTG TCACGAAAAA GCCGAAGTAC CTCACAGCTC
    AGCCAAACAG AAGTCGGAGG GAAGAAGCTG AAGAGCACCA TCCAGAGAAG TACGGAAACA
    GGCCTGGCCG TGGAAATGAG AAACTGGATG AGCCGCCAAG CAAGCAGGGA GTCCACAGAT
    GGGAGTATGA ACAGCTACAG CTCAGAGGGG AATCTGATAT TCCCTGGGGT CCGACTGGGT
    TCCGACAGCC AGTTCAGTGA CTTTTTGGAT GGTCTTGGTC CTGCCCAGCT GGTTGGTCGC
    CAGACTTTGG CCACGCCCTC TATGGGAGAT ATTCAGGTTG GAATGGCTGA CAAAAAAGGC
    CAGTTGGAGG TGGAGGTGAT ACGAGCCCGT GGTCTTGTTG TAAAACCAGG CTCAAAGACA
    CTGCCAGCAC CCTATGTTAA GGTGTATCTC TTAGAAAATG GAGTGTGCAT AGCCAAAAAG
    AAAACAAAAG TAGCAAGAAA AACGCTGGAG CCTCTATACC AGCAAGTGTT AGCGTTTGAG
    GAATCACCAC AAGGGAAGGT CCTACAGATC ATTGTTTGGG GTGACTATGG ACGCATGGAC
    CACAAGTCCT TCATGGGGGT GGCTCAGATA CTTTTAGATG AACTGGACCT ATCCAGCATG
    GTGATTGGAT GGTATAAACT TTTCCCCCCT TCATCTTTAG TAGACCCAAC GTTGGCTCCA
    CTCACTAGGA GAGCTTCCCA ATCATCTCTT GAAAGCTCCA CGGGACCTTC TTATGCTCGT
    TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031759437.1
    CDS302..4267
    Translation

    Target ORF information:

    RefSeq Version XM_031903577.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 2 (rims2), transcript variant X31, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031903577.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCAGTTCG AGACGCTGCG CCAGTTTTGC AACACGGTCC TGTCGCATTT CCACGAAGCT 
    TTCCCCACTC CGCCGAACAT ACTGCAGAAT GATCTGTTCG GGCAGCCAGG GAACAGCCCC
    GGGAAAAGAA GCCCTTCAAT ACCTAGAGAC CAGAATCGGA GATATGATAC AAGGGACGAT
    ATAGGTGAAC ATTCACAGCA TGCTACATCA GACAGCGCCA TGCCTAGATC ACCATCAGAC
    TACTCGGACA GGCGATTGCA ACGAGGGTCT CAGCTCTATG ATGAGCCTGA GCACGGTGAC
    TACAGAAGAA GTAGGCGGCG CTCCAATGAG TATTCGGTAG ATGAGGAGGA TATCCAAGAC
    AGGGAGGAAT ATGAAAGGCA GAGAAGAGAG GAGGAGTACC AGGCAAGGTA CCGGAGTGAC
    CCCAACTTGG CCAGGTACCC TGTTAAGCCA CAACCGTATG AAGAGCAAAT GCGCATCCAT
    GCAGAGGTTT CAAGGGCTCG ACAACGAAGG CACAGCGATG TGTCTTTAGC TCAGACAGAA
    TTTGAGGAGT CTCGGATGTC AACGCTGAGG ATGGAGAGAC CTTCAAGGCA AAGATCTGCG
    TCAGAGCGTA GAGCAGCGAT GGAAAGCCAG CGTTCATATT CAATAGAAAG AACTAGAGAT
    GTGCAAGGGC CAGGTCCTGG TCGGCAAAGG ACCTCAAATC ATAGTCCCCC AACACCAAGA
    AGGAGTCCCA TTCCCAATGA TAGGCCAGAT ATGAGGCGCA CAGATTCTTT ACGGAAACAG
    CACCACTTGG ACCCAAGTTC TGCCGTAAGG AAAACCAAAA GGGAAAAAAT GGAAACTATG
    TTAAGGAATG ATTCTCTTAG CTCAGATCAG TCTGAATCTG TCCGGCCCCC ACCACCAAAG
    CCTCATAAAA CAAAAAAGGG AGGTAAAATG CGTCAGGTTT CCTTGAGCAG TTCAGAAGAA
    GAGTTGGCGT CTACGCCGGA GTACACGAGC TGCGATGATG TGGAGATTGA AAGTGAAAGT
    GTTAGTGAGA AAGGTGACAT GGACTACAAC TGGGTGGAGC ATTCGTCTTG GCACAGCAGT
    GAGGCCTCCC CAATGTCTTT GCACCCTGTC ACGTGGCAAC CTTCCAAAGA TGGAGACCGC
    TTAATTGGTC GCATATTATT AAACAAGCGG CTCAAAGATG GGAGCGTCCC GCGAGATTCT
    GGAGCCATGC TGGGGCTGAA GGTCGTTGGA GGGAAAATGA CAGAATCAGG TCGACTTTGT
    GCGTTTATCA CCAAAGTGAA GAAGGGAAGC CTGGCAGATA CAGTAGGCCA TCTTCGACCA
    GGTGATGAGG TTTTAGAATG GAGTGGGCGG ATTTTGCAAG GAGCGACGTT CGAAGAAGTT
    TACAATATTA TACTGGAGTC CAAACCCGAG GCACAAGTAG AATTATTGGT CTCCAGACCC
    ATAGGAGATA TACCCCGGAT TCCTGACAGC ACACACGCAC AACTGGAATC CAGTTCTAGC
    TCATTTGAAT CCCAGAAAAT GGATCGCCCT TCTATTTCAG TTACTTCCCC CATGAGTCCT
    GGAATGTTAC GGGATGTCCC CCAGTTTCTT TCTGGACAAC TTTCAATTAA ACTCTGGTAT
    GACAAGGTTG GCCACCAGTT AATTGTCACG ATATTAGGAG CAAAGGATCT GCCATCGCGG
    GAAGATGGGA GGCCGAGAAA TCCTTACGTA AAAATTTACT TTCTTCCAGA TCGAAGTGAT
    AAGAACAAAA GGAGAACAAA GACTGTGAAG AAAACCGTAG AGCCCAAATG GAACCAAACA
    TTCATCTATT CTCCGGTTCA TCGGAGGGAA TTTCGGGAAC GAATGTTGGA AATAACACTT
    TGGGATCAAG CTCGGGTTCG AGAAGAAGAG AGTGAATTTT TAGGAGAGAT TCTAATTGAA
    TTAGAAACTG CACTATTAGA TGATGAGCCA CATTGGTACA AGCTACAGAC GCATGATGTG
    TCTTCATTGC CACTTCCACA CCCATCCCCG TTCATGCAAA GAAGGCAGCT GCACGGCGAG
    AGCCCAACCC GGAGGTTGCA AAGATCCAAA AGGATAAGCG ACAGCGAAGT GTCTGACTAC
    GACTGTGATG ATGGCGTTGG TGTTGTTTCA GACTTTCGGC ATAATGGGCG TGACCTGCAG
    AGTTCAACGT TATCTGTTCC GGAACAAGTA ATGTCGTCCA ATCACTGTTC CCGGACTGGG
    CCCCCGCATG TAGACAGCAT AGGAAGAACG AGGTCATGGT CTCCCAGTGT CCCTCCTCCG
    CAAAGTAGGA ATTTGGAACA GGGACCGAGA GGGACAAGAT CTGGCCCTGC ACATTACAAT
    ACCATGAGCA GAGTGGAAAG GCATCGAGTA TTAGATAACC ACTACTCCCC AGACAGAGAC
    AGGAATTGTG AAGCGGCAGA TAGACAGCCA TATCAAAGAT CCAGGTCCAC TGAACAACGG
    CCTGTGCTGG AGCGGACCAG CTCCCGCTCC CGATCTACTG AGCGTCCTGA CTCAAACCTC
    ATGAGGTCGA TGCCTTCATT AATGACGGGA AGATCTGCCC CTCCTTCACC CGCCTTATCG
    AGGGCACACC CTCCTCGTAC CGGCTCTGTT CAGACAAGTC CTTCAGGCAC CCCGTTAGCA
    GGGCGGAGGG GGCGCCAGCT CCCGCAACTT CCCCCAAAAG GCACGTTAGA AAGAAAAGAT
    ATGGACTCAA CGCGGAGGCG AAACTCAGGT GCTATGGACG TAGAAGAAAG GACGCGACAG
    CTGAAAGTGA AGATCAACAA ATACAAGCAG GTGGGAGGAT CAGATTCTCG GCTAGAGAAG
    GATTATCATC CAAAGTATCG GACGGGGCGG GATCCGCAGA GGGGCTCAGA CAATATGTCC
    AACAAGTCAT CAGACAGCGA TGTTAGTGAT GTGTCTGCCG TATCACGGAC CAGCAGCGCC
    TCTCGTTTTA GTAGTACGAG TTATATGTCT GTGCAGTCGG AAAGGCCTCG AGGGAACATG
    AAAATAGGTG CATTGGCATC CAAAATGCAA AGCAGGCAAA TGGGCGCCTC AGGGAAGAAC
    ATGACCAAAA GTACCAGCAT CGGAGGGGAT ATGTACACCC TGGAGAAGAA CGACGGAAGC
    CAGTCTGACA CAGCCGTGGG CACTGTGGGT GCGGGTGGAA AGAAGAGACG TTCAAGCATA
    GGCGCCAAAA TGGTGGCCAT TGTGGGCTTG TCACGAAAAA GCCGAAGTAC CTCACAGCTC
    AGCCAAACAG AAGTCGGAGG GAAGAAGCTG AAGAGCACCA TCCAGAGAAG TACGGAAACA
    GGCCTGGCCG TGGAAATGAG AAACTGGATG AGCCGCCAAG CAAGCAGGGA GTCCACAGAT
    GGGAGTATGA ACAGCTACAG CTCAGAGGGG AATCTGATAT TCCCTGGGGT CCGACTGGGT
    TCCGACAGCC AGTTCAGTGA CTTTTTGGAT GGTCTTGGTC CTGCCCAGCT GGTTGGTCGC
    CAGACTTTGG CCACGCCCTC TATGGGAGAT ATTCAGGTTG GAATGGCTGA CAAAAAAGGC
    CAGTTGGAGG TGGAGGTGAT ACGAGCCCGT GGTCTTGTTG TAAAACCAGG CTCAAAGACA
    CTGCCAGCAC CCTATGTTAA GGTGTATCTC TTAGAAAATG GAGTGTGCAT AGCCAAAAAG
    AAAACAAAAG TAGCAAGAAA AACGCTGGAG CCTCTATACC AGCAAGTGTT AGCGTTTGAG
    GAATCACCAC AAGGGAAGGT CCTACAGATC ATTGTTTGGG GTGACTATGG ACGCATGGAC
    CACAAGTCCT TCATGGGGGT GGCTCAGATA CTTTTAGATG AACTGGACCT ATCCAGCATG
    GTGATTGGAT GGTATAAACT TTTCCCCCCT TCATCTTTAG TAGACCCAAC GTTGGCTCCA
    CTCACTAGGA GAGCTTCCCA ATCATCTCTT GAAAGCTCCA CGGGACCTTC TTATGCTCGT
    TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa59926
    Clone ID Related Accession (Same CDS sequence) XM_031903581.1
    Accession Version XM_031903581.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4200bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 2 isoform X38
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTCTGCTC CACTAGGGCC CAGGGGCGGC CCTCTGCCTC CTGCACAGCC ACAGGCTCCA 
    ATGCCAGAGA TGCCGGACCT CAGCCACCTG ACAGAGGATG AGAGGAAAAT CATAATGGCT
    GTGATGGAGA GGCAGAAGAA AGAAGAGGAG AAGGAGCAGT CAGTGTTAAA AAAATTGCAC
    CAGCAGTTTG AAATGTACAA GGAGCAAGTG AAGAAGATGG GCGAGGAAGC ACAGCAGCAG
    CAGGAGCAGA AGGGCGAAGC TCCAACCTGT GGAATCTGCC ACAAAACAAA GTTTGCTGAT
    GGCTGTGGCC ACGACTGTTC GTATTGCCAA ACTAAATTCT GCGCGCGGTG TGGAGGGCGA
    GTGTCTCTGC GCTCAAATAA GGAGGACAAA GTGGTAATGT GGGTATGTAA TTTGTGCCGA
    AAACAACAAG AAATCCTCAC CAAATCAGGG GCCTGGTTTT ACAGCAGTGG GTCAAGCACA
    CCCCTAAAGC CCGGCGAAGA GAGCCAGGCA GGGTACCAAA CGGAAGAAGC ACCTCAGGAG
    AAAAAAGCTA AGCTACAAGA GCAGTCCCAA TACCAGGGAC CCTCAGGGGA CTTATTAACA
    CCAGCCTCGG ACAAAAACCG ACCGCAAGGG ATCGTCCGGC AAGATTCCAT CAAAAATGGT
    TCCGAACTGA AGCACCCGAT TCCTGGCGAC CTCGCATCTG ACAGGAAAAG AAGCCCTTCA
    ATACCTAGAG ACCAGAATCG GAGATATGAT ACAAGGGACG ATATAGGTGA ACATTCACAG
    CATGCTACAT CAGACAGCGC CATGCCTAGA TCACCATCAG ACTACTCGGA CAGGCGATTG
    CAACGAGGGT CTCAGCTCTA TGATGAGCCT GAGCACGGTG ACTACAGAAG AAGTAGGCGG
    CGCTCCAATG AGTATTCGGT AGATGAGGAG GATATCCAAG ACAGGGAGGA ATATGAAAGG
    CAGAGAAGAG AGGAGGAGTA CCAGGCAAGG TACCGGAGTG ACCCCAACTT GGCCAGGTAC
    CCTGTTAAGC CACAACCGTA TGAAGAGCAA ATGCGCATCC ATGCAGAGGT TTCAAGGGCT
    CGACAACGAA GGCACAGCGA TGTGTCTTTA GCTCAGACAG AATTTGAGGA GTCTCGGATG
    TCAACGCTGA GGATGGAGAG ACCTTCAAGG CAAAGATCTG CGTCAGAGCG TAGAGCAGCG
    ATGGAAAGCC AGCGTTCATA TTCAATAGAA AGAACTAGAG ATGTGCAAGG GCCAGGTCCT
    GGTCGGCAAA GGACCTCAAA TCATAGTCCC CCAACACCAA GAAGGAGTCC CATTCCCAAT
    GATAGGCCAG ATATGAGGCG CACAGATTCT TTACGGAAAC AGCACCACTT GGACCCAAGT
    TCTGCCGTAA GGAAAACCAA AAGGGAAAAA ATGGAAACTA TGTTAAGGAA TGATTCTCTT
    AGCTCAGATC AGTCTGAATC TGTCCGGCCC CCACCACCAA AGCCTCATAA AACAAAAAAG
    GGAGGTAAAA TGCGTCAGGT TTCCTTGAGC AGTTCAGAAG AAGAGTTGGC GTCTACGCCG
    GAGTACACGA GCTGCGATGA TGTGGAGATT GAAAGTGAAA GTGTTAGTGA GAAAGGGGAC
    AGTCAAAGGG GAAAAAGAAA AACTAGTGAG CAGGCAGTTT TGTCGGATTC TAACATCTTG
    TCTGAGAGAC AAAAGAAAAT GGTGTGCTTT GGAGGCCACT CTTTGGAAGA GGATTTGGAA
    TGGTCTGAGC CTCAGATTAA AGACTCTGGG GTAGACACGT GTAGTAGCAC CACCCTTAAC
    GAAGAGCATA GCCATAGCGA GAAGCACCCT GTCACGTGGC AACCTTCCAA AGATGGAGAC
    CGCTTAATTG GTCGCATATT ATTAAACAAG CGGCTCAAAG ATGGGAGCGT CCCGCGAGAT
    TCTGGAGCCA TGCTGGGGCT GAAGGTCGTT GGAGGGAAAA TGACAGAATC AGGTCGACTT
    TGTGCGTTTA TCACCAAAGT GAAGAAGGGA AGCCTGGCAG ATACAGTAGG CCATCTTCGA
    CCAGGTGATG AGGTTTTAGA ATGGAGTGGG CGGATTTTGC AAGGAGCGAC GTTCGAAGAA
    GTTTACAATA TTATACTGGA GTCCAAACCC GAGGCACAAG TAGAATTATT GGTCTCCAGA
    CCCATAGGAG ATATACCCCG GATTCCTGAC AGCACACACG CACAACTGGA ATCCAGTTCT
    AGCTCATTTG AATCCCAGAA AATGGATCGC CCTTCTATTT CAGTTACTTC CCCCATGAGT
    CCTGGAATGT TACGGGATGT CCCCCAGTTT CTTTCTGGAC AACTTTCAAT TAAACTCTGG
    TATGACAAGG TTGGCCACCA GTTAATTGTC ACGATATTAG GAGCAAAGGA TCTGCCATCG
    CGGGAAGATG GGAGGCCGAG AAATCCTTAC GTAAAAATTT ACTTTCTTCC AGATCGAAGT
    GATAAGAACA AAAGGAGAAC AAAGACTGTG AAGAAAACCG TAGAGCCCAA ATGGAACCAA
    ACATTCATCT ATTCTCCGGT TCATCGGAGG GAATTTCGGG AACGAATGTT GGAAATAACA
    CTTTGGGATC AAGCTCGGGT TCGAGAAGAA GAGAGTGAAT TTTTAGGAGA GATTCTAATT
    GAATTAGAAA CTGCACTATT AGATGATGAG CCACATTGGT ACAAGCTACA GACGCATGAT
    GTGTCTTCAT TGCCACTTCC ACACCCATCC CCGTTCATGC AAAGAAGGCA GCTGCACGGC
    GAGAGCCCAA CCCGGAGGTT GCAAAGATCC AAAAGGATAA GCGACAGCGA AGTGTCTGAC
    TACGACTGTG ATGATGGCGT TGGTGTTGTT TCAGACTTTC GGCATAATGG GCGTGACCTG
    CAGAGTTCAA CGTTATCTGT TCCGGAACAA GTAATGTCGT CCAATCACTG TTCCCGGACT
    GGGCCCCCGC ATGTAGACAG CATAGGAAGA ACGAGGTCAT GGTCTCCCAG TGTCCCTCCT
    CCGCAAAGTA GGAATTTGGA ACAGGGACCG AGAGGGACAA GATCTGGCCC TGCACATTAC
    AATACCATGA GCAGAGTGGA AAGGCATCGA GTATTAGATA ACCACTACTC CCCAGACAGA
    GACAGTCATT ATCTTACTCT ACCTCGCACC AGATTCAGTC AGTCCTTTGA CCATCACCTA
    GCAGAGCCAA GGAATTGTGA AGCGGCAGAT AGACAGCCAT ATCAAAGATC CAGGTCCACT
    GAACAACGGC CTGTGCTGGA GCGGACCAGC TCCCGCTCCC GATCTACTGA GCGTCCTGAC
    TCAAACCTCA TGAGGTCGAT GCCTTCATTA ATGACGGGAA GATCTGCCCC TCCTTCACCC
    GCCTTATCGA GGGCACACCC TCCTCGTACC GGCTCTGTTC AGACAAGTCC TTCAGGCACC
    CCGTTAGCAG GGCGGAGGGG GCGCCAGCTC CCGCAACTTC CCCCAAAAGG CACGTTAGAA
    AGAAAAGTCG GAGGGAAGAA GCTGAAGAGC ACCATCCAGA GAAGTACGGA AACAGGCCTG
    GCCGTGGAAA TGAGAAACTG GATGAGCCGC CAAGCAAGCA GGGAGTCCAC AGATGGGAGT
    ATGAACAGCT ACAGCTCAGA GGGGAATCTG ATATTCCCTG GGGTCCGACT GGGTTCCGAC
    AGCCAGTTCA GTGACTTTTT GGATGGTCTT GGTCCTGCCC AGCTGGTTGG TCGCCAGACT
    TTGGCCACGC CCTCTATGGG AGATATTCAG GTTGGAATGG CTGACAAAAA AGGCCAGTTG
    GAGGTGGAGG TGATACGAGC CCGTGGTCTT GTTGTAAAAC CAGGCTCAAA GACACTGCCA
    GCACCCTATG TTAAGGTGTA TCTCTTAGAA AATGGAGTGT GCATAGCCAA AAAGAAAACA
    AAAGTAGCAA GAAAAACGCT GGAGCCTCTA TACCAGCAAG TGTTAGCGTT TGAGGAATCA
    CCACAAGGGA AGGTCCTACA GATCATTGTT TGGGGTGACT ATGGACGCAT GGACCACAAG
    TCCTTCATGG GGGTGGCTCA GATACTTTTA GATGAACTGG ACCTATCCAG CATGGTGATT
    GGATGGTATA AACTTTTCCC CCCTTCATCT TTAGTAGACC CAACGTTGGC TCCACTCACT
    AGGAGAGCTT CCCAATCATC TCTTGAAAGC TCCACGGGAC CTTCTTATGC TCGTTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031759441.1
    CDS420..4619
    Translation

    Target ORF information:

    RefSeq Version XM_031903581.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 2 (rims2), transcript variant X38, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031903581.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTCTGCTC CACTAGGGCC CAGGGGCGGC CCTCTGCCTC CTGCACAGCC ACAGGCTCCA 
    ATGCCAGAGA TGCCGGACCT CAGCCACCTG ACAGAGGATG AGAGGAAAAT CATAATGGCT
    GTGATGGAGA GGCAGAAGAA AGAAGAGGAG AAGGAGCAGT CAGTGTTAAA AAAATTGCAC
    CAGCAGTTTG AAATGTACAA GGAGCAAGTG AAGAAGATGG GCGAGGAAGC ACAGCAGCAG
    CAGGAGCAGA AGGGCGAAGC TCCAACCTGT GGAATCTGCC ACAAAACAAA GTTTGCTGAT
    GGCTGTGGCC ACGACTGTTC GTATTGCCAA ACTAAATTCT GCGCGCGGTG TGGAGGGCGA
    GTGTCTCTGC GCTCAAATAA GGAGGACAAA GTGGTAATGT GGGTATGTAA TTTGTGCCGA
    AAACAACAAG AAATCCTCAC CAAATCAGGG GCCTGGTTTT ACAGCAGTGG GTCAAGCACA
    CCCCTAAAGC CCGGCGAAGA GAGCCAGGCA GGGTACCAAA CGGAAGAAGC ACCTCAGGAG
    AAAAAAGCTA AGCTACAAGA GCAGTCCCAA TACCAGGGAC CCTCAGGGGA CTTATTAACA
    CCAGCCTCGG ACAAAAACCG ACCGCAAGGG ATCGTCCGGC AAGATTCCAT CAAAAATGGT
    TCCGAACTGA AGCACCCGAT TCCTGGCGAC CTCGCATCTG ACAGGAAAAG AAGCCCTTCA
    ATACCTAGAG ACCAGAATCG GAGATATGAT ACAAGGGACG ATATAGGTGA ACATTCACAG
    CATGCTACAT CAGACAGCGC CATGCCTAGA TCACCATCAG ACTACTCGGA CAGGCGATTG
    CAACGAGGGT CTCAGCTCTA TGATGAGCCT GAGCACGGTG ACTACAGAAG AAGTAGGCGG
    CGCTCCAATG AGTATTCGGT AGATGAGGAG GATATCCAAG ACAGGGAGGA ATATGAAAGG
    CAGAGAAGAG AGGAGGAGTA CCAGGCAAGG TACCGGAGTG ACCCCAACTT GGCCAGGTAC
    CCTGTTAAGC CACAACCGTA TGAAGAGCAA ATGCGCATCC ATGCAGAGGT TTCAAGGGCT
    CGACAACGAA GGCACAGCGA TGTGTCTTTA GCTCAGACAG AATTTGAGGA GTCTCGGATG
    TCAACGCTGA GGATGGAGAG ACCTTCAAGG CAAAGATCTG CGTCAGAGCG TAGAGCAGCG
    ATGGAAAGCC AGCGTTCATA TTCAATAGAA AGAACTAGAG ATGTGCAAGG GCCAGGTCCT
    GGTCGGCAAA GGACCTCAAA TCATAGTCCC CCAACACCAA GAAGGAGTCC CATTCCCAAT
    GATAGGCCAG ATATGAGGCG CACAGATTCT TTACGGAAAC AGCACCACTT GGACCCAAGT
    TCTGCCGTAA GGAAAACCAA AAGGGAAAAA ATGGAAACTA TGTTAAGGAA TGATTCTCTT
    AGCTCAGATC AGTCTGAATC TGTCCGGCCC CCACCACCAA AGCCTCATAA AACAAAAAAG
    GGAGGTAAAA TGCGTCAGGT TTCCTTGAGC AGTTCAGAAG AAGAGTTGGC GTCTACGCCG
    GAGTACACGA GCTGCGATGA TGTGGAGATT GAAAGTGAAA GTGTTAGTGA GAAAGGGGAC
    AGTCAAAGGG GAAAAAGAAA AACTAGTGAG CAGGCAGTTT TGTCGGATTC TAACATCTTG
    TCTGAGAGAC AAAAGAAAAT GGTGTGCTTT GGAGGCCACT CTTTGGAAGA GGATTTGGAA
    TGGTCTGAGC CTCAGATTAA AGACTCTGGG GTAGACACGT GTAGTAGCAC CACCCTTAAC
    GAAGAGCATA GCCATAGCGA GAAGCACCCT GTCACGTGGC AACCTTCCAA AGATGGAGAC
    CGCTTAATTG GTCGCATATT ATTAAACAAG CGGCTCAAAG ATGGGAGCGT CCCGCGAGAT
    TCTGGAGCCA TGCTGGGGCT GAAGGTCGTT GGAGGGAAAA TGACAGAATC AGGTCGACTT
    TGTGCGTTTA TCACCAAAGT GAAGAAGGGA AGCCTGGCAG ATACAGTAGG CCATCTTCGA
    CCAGGTGATG AGGTTTTAGA ATGGAGTGGG CGGATTTTGC AAGGAGCGAC GTTCGAAGAA
    GTTTACAATA TTATACTGGA GTCCAAACCC GAGGCACAAG TAGAATTATT GGTCTCCAGA
    CCCATAGGAG ATATACCCCG GATTCCTGAC AGCACACACG CACAACTGGA ATCCAGTTCT
    AGCTCATTTG AATCCCAGAA AATGGATCGC CCTTCTATTT CAGTTACTTC CCCCATGAGT
    CCTGGAATGT TACGGGATGT CCCCCAGTTT CTTTCTGGAC AACTTTCAAT TAAACTCTGG
    TATGACAAGG TTGGCCACCA GTTAATTGTC ACGATATTAG GAGCAAAGGA TCTGCCATCG
    CGGGAAGATG GGAGGCCGAG AAATCCTTAC GTAAAAATTT ACTTTCTTCC AGATCGAAGT
    GATAAGAACA AAAGGAGAAC AAAGACTGTG AAGAAAACCG TAGAGCCCAA ATGGAACCAA
    ACATTCATCT ATTCTCCGGT TCATCGGAGG GAATTTCGGG AACGAATGTT GGAAATAACA
    CTTTGGGATC AAGCTCGGGT TCGAGAAGAA GAGAGTGAAT TTTTAGGAGA GATTCTAATT
    GAATTAGAAA CTGCACTATT AGATGATGAG CCACATTGGT ACAAGCTACA GACGCATGAT
    GTGTCTTCAT TGCCACTTCC ACACCCATCC CCGTTCATGC AAAGAAGGCA GCTGCACGGC
    GAGAGCCCAA CCCGGAGGTT GCAAAGATCC AAAAGGATAA GCGACAGCGA AGTGTCTGAC
    TACGACTGTG ATGATGGCGT TGGTGTTGTT TCAGACTTTC GGCATAATGG GCGTGACCTG
    CAGAGTTCAA CGTTATCTGT TCCGGAACAA GTAATGTCGT CCAATCACTG TTCCCGGACT
    GGGCCCCCGC ATGTAGACAG CATAGGAAGA ACGAGGTCAT GGTCTCCCAG TGTCCCTCCT
    CCGCAAAGTA GGAATTTGGA ACAGGGACCG AGAGGGACAA GATCTGGCCC TGCACATTAC
    AATACCATGA GCAGAGTGGA AAGGCATCGA GTATTAGATA ACCACTACTC CCCAGACAGA
    GACAGTCATT ATCTTACTCT ACCTCGCACC AGATTCAGTC AGTCCTTTGA CCATCACCTA
    GCAGAGCCAA GGAATTGTGA AGCGGCAGAT AGACAGCCAT ATCAAAGATC CAGGTCCACT
    GAACAACGGC CTGTGCTGGA GCGGACCAGC TCCCGCTCCC GATCTACTGA GCGTCCTGAC
    TCAAACCTCA TGAGGTCGAT GCCTTCATTA ATGACGGGAA GATCTGCCCC TCCTTCACCC
    GCCTTATCGA GGGCACACCC TCCTCGTACC GGCTCTGTTC AGACAAGTCC TTCAGGCACC
    CCGTTAGCAG GGCGGAGGGG GCGCCAGCTC CCGCAACTTC CCCCAAAAGG CACGTTAGAA
    AGAAAAGTCG GAGGGAAGAA GCTGAAGAGC ACCATCCAGA GAAGTACGGA AACAGGCCTG
    GCCGTGGAAA TGAGAAACTG GATGAGCCGC CAAGCAAGCA GGGAGTCCAC AGATGGGAGT
    ATGAACAGCT ACAGCTCAGA GGGGAATCTG ATATTCCCTG GGGTCCGACT GGGTTCCGAC
    AGCCAGTTCA GTGACTTTTT GGATGGTCTT GGTCCTGCCC AGCTGGTTGG TCGCCAGACT
    TTGGCCACGC CCTCTATGGG AGATATTCAG GTTGGAATGG CTGACAAAAA AGGCCAGTTG
    GAGGTGGAGG TGATACGAGC CCGTGGTCTT GTTGTAAAAC CAGGCTCAAA GACACTGCCA
    GCACCCTATG TTAAGGTGTA TCTCTTAGAA AATGGAGTGT GCATAGCCAA AAAGAAAACA
    AAAGTAGCAA GAAAAACGCT GGAGCCTCTA TACCAGCAAG TGTTAGCGTT TGAGGAATCA
    CCACAAGGGA AGGTCCTACA GATCATTGTT TGGGGTGACT ATGGACGCAT GGACCACAAG
    TCCTTCATGG GGGTGGCTCA GATACTTTTA GATGAACTGG ACCTATCCAG CATGGTGATT
    GGATGGTATA AACTTTTCCC CCCTTCATCT TTAGTAGACC CAACGTTGGC TCCACTCACT
    AGGAGAGCTT CCCAATCATC TCTTGAAAGC TCCACGGGAC CTTCTTATGC TCGTTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa59928
    Clone ID Related Accession (Same CDS sequence) XM_031903583.1
    Accession Version XM_031903583.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4062bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 2 isoform X40
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCAGTTCG AGACGCTGCG CCAGTTTTGC AACACGGTCC TGTCGCATTT CCACGAAGCT 
    TTCCCCACTC CGCCGAACAT ACTGCAGAAT GATCTGTTCG GGCAGCCAGG GAACAGCCCC
    GGGAAAAGAA GCCCTTCAAT ACCTAGAGAC CAGAATCGGA GATATGATAC AAGGGACGAT
    ATAGGTGAAC ATTCACAGCA TGCTACATCA GACAGCGCCA TGCCTAGATC ACCATCAGAC
    TACTCGGACA GGCGATTGCA ACGAGGGTCT CAGCTCTATG ATGAGCCTGA GCACGGTGAC
    TACAGAAGAA GTAGGCGGCG CTCCAATGAG TATTCGGTAG ATGAGGAGGA TATCCAAGAC
    AGGGAGGAAT ATGAAAGGCA GAGAAGAGAG GAGGAGTACC AGGCAAGGTA CCGGAGTGAC
    CCCAACTTGG CCAGGTACCC TGTTAAGCCA CAACCGTATG AAGAGCAAAT GCGCATCCAT
    GCAGAGGTTT CAAGGGCTCG ACAACGAAGG CACAGCGATG TGTCTTTAGC TCAGACAGAA
    TTTGAGGAGT CTCGGATGTC AACGCTGAGG ATGGAGAGAC CTTCAAGGCA AAGATCTGCG
    TCAGAGCGTA GAGCAGCGAT GGAAAGCCAG CGTTCATATT CAATAGAAAG AACTAGAGAT
    GTGCAAGGGC CAGGTCCTGG TCGGCAAAGG ACCTCAAATC ATAGTCCCCC AACACCAAGA
    AGGAGTCCCA TTCCCAATGA TAGGCCAGAT ATGAGGCGCA CAGATTCTTT ACGGAAACAG
    CACCACTTGG ACCCAAGTTC TGCCGTAAGG AAAACCAAAA GGGAAAAAAT GGAAACTATG
    TTAAGGAATG ATTCTCTTAG CTCAGATCAG TCTGAATCTG TCCGGCCCCC ACCACCAAAG
    CCTCATAAAA CAAAAAAGGG AGGTAAAATG CGTCAGGTTT CCTTGAGCAG TTCAGAAGAA
    GAGTTGGCGT CTACGCCGGA GTACACGAGC TGCGATGATG TGGAGATTGA AAGTGAAAGT
    GTTAGTGAGA AAGGTGACAT GGACTACAAC TGGGTGGAGC ATTCGTCTTG GCACAGCAGT
    GAGGCCTCCC CAATGTCTTT GCACCCTGTC ACGTGGCAAC CTTCCAAAGA TGGAGACCGC
    TTAATTGGTC GCATATTATT AAACAAGCGG CTCAAAGATG GGAGCGTCCC GCGAGATTCT
    GGAGCCATGC TGGGGCTGAA GGTCGTTGGA GGGAAAATGA CAGAATCAGG TCGACTTTGT
    GCGTTTATCA CCAAAGTGAA GAAGGGAAGC CTGGCAGATA CAGTAGGCCA TCTTCGACCA
    GGTGATGAGG TTTTAGAATG GAGTGGGCGG ATTTTGCAAG GAGCGACGTT CGAAGAAGTT
    TACAATATTA TACTGGAGTC CAAACCCGAG GCACAAGTAG AATTATTGGT CTCCAGACCC
    ATAGGAGATA TACCCCGGAT TCCTGACAGC ACACACGCAC AACTGGAATC CAGTTCTAGC
    TCATTTGAAT CCCAGAAAAT GGATCGCCCT TCTATTTCAG TTACTTCCCC CATGAGTCCT
    GGAATGTTAC GGGATGTCCC CCAGTTTCTT TCTGGACAAC TTTCAATTAA ACTCTGGTAT
    GACAAGGTTG GCCACCAGTT AATTGTCACG ATATTAGGAG CAAAGGATCT GCCATCGCGG
    GAAGATGGGA GGCCGAGAAA TCCTTACGTA AAAATTTACT TTCTTCCAGA TCGAAGTGAT
    AAGAACAAAA GGAGAACAAA GACTGTGAAG AAAACCGTAG AGCCCAAATG GAACCAAACA
    TTCATCTATT CTCCGGTTCA TCGGAGGGAA TTTCGGGAAC GAATGTTGGA AATAACACTT
    TGGGATCAAG CTCGGGTTCG AGAAGAAGAG AGTGAATTTT TAGGAGAGAT TCTAATTGAA
    TTAGAAACTG CACTATTAGA TGATGAGCCA CATTGGTACA AGCTACAGAC GCATGATGTG
    TCTTCATTGC CACTTCCACA CCCATCCCCG TTCATGCAAA GAAGGCAGCT GCACGGCGAG
    AGCCCAACCC GGAGGTTGCA AAGATCCAAA AGGATAAGCG ACAGCGAAGT GTCTGACTAC
    GACTGTGATG ATGGCGTTGG TGTTGTTTCA GACTTTCGGC ATAATGGGCG TGACCTGCAG
    AGTTCAACGT TATCTGTTCC GGAACAAGTA ATGTCGTCCA ATCACTGTTC CCGGACTGGG
    CCCCCGCATG TAGACAGCAT AGGAAGAACG AGGTCATGGT CTCCCAGTGT CCCTCCTCCG
    CAAAGTAGGA ATTTGGAACA GGGACCGAGA GGGACAAGAT CTGGCCCTGC ACATTACAAT
    ACCATGAGCA GAGTGGAAAG GCATCGAGTA TTAGATAACC ACTACTCCCC AGACAGAGAC
    AGTCATTATC TTACTCTACC TCGCACCAGA TTCAGTCAGT CCTTTGACCA TCACCTAGCA
    GAGCCAAGGA ATTGTGAAGC GGCAGATAGA CAGCCATATC AAAGATCCAG GTCCACTGAA
    CAACGGCCTG TGCTGGAGCG GACCAGCTCC CGCTCCCGAT CTACTGAGCG TCCTGACTCA
    AACCTCATGA GGTCGATGCC TTCATTAATG ACGGGAAGAT CTGCCCCTCC TTCACCCGCC
    TTATCGAGGG CACACCCTCC TCGTACCGGC TCTGTTCAGA CAAGTCCTTC AGGCACCCCG
    TTAGCAGGGC GGAGGGGGCG CCAGCTCCCG CAACTTCCCC CAAAAGGCAC GTTAGAAAGA
    AAAAATGGAG ACAAGGAAAT AGAGCTTTAT GAAGATATGG ACTCAACGCG GAGGCGAAAC
    TCAGGTGCTA TGGACGTAGA AGAAAGGACG CGACAGCTGA AAGTGAAGAT CAACAAATAC
    AAGCAGGTGG GAGGATCAGA TTCTCGGCTA GAGAAGGATT ATCATCCAAA GTATCGGACG
    GGGCGGGATC CGCAGAGGGG CTCAGACAAT ATGTCCAACA AGTCATCAGA CAGCGATGTT
    AGTGATGTGT CTGCCGTATC ACGGACCAGC AGCGCCTCTC GTTTTAGTAG TACGAGTTAT
    ATGTCTGTGC AGTCGGAAAG GCCTCGAGGG AACATGAAAA TAGGTGCATT GGCATCCAAA
    ATGCAAAGCA GGCAAATGGG CGCCTCAGGG AAGAACATGA CCAAAAGTAC CAGCATCGGA
    GGGGATATGT ACACCCTGGA GAAGAACGAC GGAAGCCAGT CTGACACAGC CGTGGGCACT
    GTGGGTGCGG GTGGAAAGAA GAGACGTTCA AGCATAGGCG CCAAAATGGT GGCCATTGTG
    GGCTTGTCAC GAAAAAGCCG AAGTACCTCA CAGCTCAGCC AAACAGAAGT CGGAGGGAAG
    AAGCTGAAGA GCACCATCCA GAGAAGTACG GAAACAGGCC TGGCCGTGGA AATGAGAAAC
    TGGATGAGCC GCCAAGCAAG CAGGGAGTCC ACAGATGGGA GTATGAACAG CTACAGCTCA
    GAGGGGAATC TGATATTCCC TGGGGTCCGA CTGGGTTCCG ACAGCCAGTT CAGTGACTTT
    TTGGATGGTC TTGGTCCTGC CCAGCTGGTT GGTCGCCAGA CTTTGGCCAC GCCCTCTATG
    GGAGATATTC AGGTTGGAAT GGCTGACAAA AAAGGCCAGT TGGAGGTGGA GGTGATACGA
    GCCCGTGGTC TTGTTGTAAA ACCAGGCTCA AAGACACTGC CAGCACCCTA TGTTAAGGTG
    TATCTCTTAG AAAATGGAGT GTGCATAGCC AAAAAGAAAA CAAAAGTAGC AAGAAAAACG
    CTGGAGCCTC TATACCAGCA AGTGTTAGCG TTTGAGGAAT CACCACAAGG GAAGGTCCTA
    CAGATCATTG TTTGGGGTGA CTATGGACGC ATGGACCACA AGTCCTTCAT GGGGGTGGCT
    CAGATACTTT TAGATGAACT GGACCTATCC AGCATGGTGA TTGGATGGTA TAAACTTTTC
    CCCCCTTCAT CTTTAGTAGA CCCAACGTTG GCTCCACTCA CTAGGAGAGC TTCCCAATCA
    TCTCTTGAAA GCTCCACGGG ACCTTCTTAT GCTCGTTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031759443.1
    CDS305..4366
    Translation

    Target ORF information:

    RefSeq Version XM_031903583.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 2 (rims2), transcript variant X40, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031903583.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCAGTTCG AGACGCTGCG CCAGTTTTGC AACACGGTCC TGTCGCATTT CCACGAAGCT 
    TTCCCCACTC CGCCGAACAT ACTGCAGAAT GATCTGTTCG GGCAGCCAGG GAACAGCCCC
    GGGAAAAGAA GCCCTTCAAT ACCTAGAGAC CAGAATCGGA GATATGATAC AAGGGACGAT
    ATAGGTGAAC ATTCACAGCA TGCTACATCA GACAGCGCCA TGCCTAGATC ACCATCAGAC
    TACTCGGACA GGCGATTGCA ACGAGGGTCT CAGCTCTATG ATGAGCCTGA GCACGGTGAC
    TACAGAAGAA GTAGGCGGCG CTCCAATGAG TATTCGGTAG ATGAGGAGGA TATCCAAGAC
    AGGGAGGAAT ATGAAAGGCA GAGAAGAGAG GAGGAGTACC AGGCAAGGTA CCGGAGTGAC
    CCCAACTTGG CCAGGTACCC TGTTAAGCCA CAACCGTATG AAGAGCAAAT GCGCATCCAT
    GCAGAGGTTT CAAGGGCTCG ACAACGAAGG CACAGCGATG TGTCTTTAGC TCAGACAGAA
    TTTGAGGAGT CTCGGATGTC AACGCTGAGG ATGGAGAGAC CTTCAAGGCA AAGATCTGCG
    TCAGAGCGTA GAGCAGCGAT GGAAAGCCAG CGTTCATATT CAATAGAAAG AACTAGAGAT
    GTGCAAGGGC CAGGTCCTGG TCGGCAAAGG ACCTCAAATC ATAGTCCCCC AACACCAAGA
    AGGAGTCCCA TTCCCAATGA TAGGCCAGAT ATGAGGCGCA CAGATTCTTT ACGGAAACAG
    CACCACTTGG ACCCAAGTTC TGCCGTAAGG AAAACCAAAA GGGAAAAAAT GGAAACTATG
    TTAAGGAATG ATTCTCTTAG CTCAGATCAG TCTGAATCTG TCCGGCCCCC ACCACCAAAG
    CCTCATAAAA CAAAAAAGGG AGGTAAAATG CGTCAGGTTT CCTTGAGCAG TTCAGAAGAA
    GAGTTGGCGT CTACGCCGGA GTACACGAGC TGCGATGATG TGGAGATTGA AAGTGAAAGT
    GTTAGTGAGA AAGGTGACAT GGACTACAAC TGGGTGGAGC ATTCGTCTTG GCACAGCAGT
    GAGGCCTCCC CAATGTCTTT GCACCCTGTC ACGTGGCAAC CTTCCAAAGA TGGAGACCGC
    TTAATTGGTC GCATATTATT AAACAAGCGG CTCAAAGATG GGAGCGTCCC GCGAGATTCT
    GGAGCCATGC TGGGGCTGAA GGTCGTTGGA GGGAAAATGA CAGAATCAGG TCGACTTTGT
    GCGTTTATCA CCAAAGTGAA GAAGGGAAGC CTGGCAGATA CAGTAGGCCA TCTTCGACCA
    GGTGATGAGG TTTTAGAATG GAGTGGGCGG ATTTTGCAAG GAGCGACGTT CGAAGAAGTT
    TACAATATTA TACTGGAGTC CAAACCCGAG GCACAAGTAG AATTATTGGT CTCCAGACCC
    ATAGGAGATA TACCCCGGAT TCCTGACAGC ACACACGCAC AACTGGAATC CAGTTCTAGC
    TCATTTGAAT CCCAGAAAAT GGATCGCCCT TCTATTTCAG TTACTTCCCC CATGAGTCCT
    GGAATGTTAC GGGATGTCCC CCAGTTTCTT TCTGGACAAC TTTCAATTAA ACTCTGGTAT
    GACAAGGTTG GCCACCAGTT AATTGTCACG ATATTAGGAG CAAAGGATCT GCCATCGCGG
    GAAGATGGGA GGCCGAGAAA TCCTTACGTA AAAATTTACT TTCTTCCAGA TCGAAGTGAT
    AAGAACAAAA GGAGAACAAA GACTGTGAAG AAAACCGTAG AGCCCAAATG GAACCAAACA
    TTCATCTATT CTCCGGTTCA TCGGAGGGAA TTTCGGGAAC GAATGTTGGA AATAACACTT
    TGGGATCAAG CTCGGGTTCG AGAAGAAGAG AGTGAATTTT TAGGAGAGAT TCTAATTGAA
    TTAGAAACTG CACTATTAGA TGATGAGCCA CATTGGTACA AGCTACAGAC GCATGATGTG
    TCTTCATTGC CACTTCCACA CCCATCCCCG TTCATGCAAA GAAGGCAGCT GCACGGCGAG
    AGCCCAACCC GGAGGTTGCA AAGATCCAAA AGGATAAGCG ACAGCGAAGT GTCTGACTAC
    GACTGTGATG ATGGCGTTGG TGTTGTTTCA GACTTTCGGC ATAATGGGCG TGACCTGCAG
    AGTTCAACGT TATCTGTTCC GGAACAAGTA ATGTCGTCCA ATCACTGTTC CCGGACTGGG
    CCCCCGCATG TAGACAGCAT AGGAAGAACG AGGTCATGGT CTCCCAGTGT CCCTCCTCCG
    CAAAGTAGGA ATTTGGAACA GGGACCGAGA GGGACAAGAT CTGGCCCTGC ACATTACAAT
    ACCATGAGCA GAGTGGAAAG GCATCGAGTA TTAGATAACC ACTACTCCCC AGACAGAGAC
    AGTCATTATC TTACTCTACC TCGCACCAGA TTCAGTCAGT CCTTTGACCA TCACCTAGCA
    GAGCCAAGGA ATTGTGAAGC GGCAGATAGA CAGCCATATC AAAGATCCAG GTCCACTGAA
    CAACGGCCTG TGCTGGAGCG GACCAGCTCC CGCTCCCGAT CTACTGAGCG TCCTGACTCA
    AACCTCATGA GGTCGATGCC TTCATTAATG ACGGGAAGAT CTGCCCCTCC TTCACCCGCC
    TTATCGAGGG CACACCCTCC TCGTACCGGC TCTGTTCAGA CAAGTCCTTC AGGCACCCCG
    TTAGCAGGGC GGAGGGGGCG CCAGCTCCCG CAACTTCCCC CAAAAGGCAC GTTAGAAAGA
    AAAAATGGAG ACAAGGAAAT AGAGCTTTAT GAAGATATGG ACTCAACGCG GAGGCGAAAC
    TCAGGTGCTA TGGACGTAGA AGAAAGGACG CGACAGCTGA AAGTGAAGAT CAACAAATAC
    AAGCAGGTGG GAGGATCAGA TTCTCGGCTA GAGAAGGATT ATCATCCAAA GTATCGGACG
    GGGCGGGATC CGCAGAGGGG CTCAGACAAT ATGTCCAACA AGTCATCAGA CAGCGATGTT
    AGTGATGTGT CTGCCGTATC ACGGACCAGC AGCGCCTCTC GTTTTAGTAG TACGAGTTAT
    ATGTCTGTGC AGTCGGAAAG GCCTCGAGGG AACATGAAAA TAGGTGCATT GGCATCCAAA
    ATGCAAAGCA GGCAAATGGG CGCCTCAGGG AAGAACATGA CCAAAAGTAC CAGCATCGGA
    GGGGATATGT ACACCCTGGA GAAGAACGAC GGAAGCCAGT CTGACACAGC CGTGGGCACT
    GTGGGTGCGG GTGGAAAGAA GAGACGTTCA AGCATAGGCG CCAAAATGGT GGCCATTGTG
    GGCTTGTCAC GAAAAAGCCG AAGTACCTCA CAGCTCAGCC AAACAGAAGT CGGAGGGAAG
    AAGCTGAAGA GCACCATCCA GAGAAGTACG GAAACAGGCC TGGCCGTGGA AATGAGAAAC
    TGGATGAGCC GCCAAGCAAG CAGGGAGTCC ACAGATGGGA GTATGAACAG CTACAGCTCA
    GAGGGGAATC TGATATTCCC TGGGGTCCGA CTGGGTTCCG ACAGCCAGTT CAGTGACTTT
    TTGGATGGTC TTGGTCCTGC CCAGCTGGTT GGTCGCCAGA CTTTGGCCAC GCCCTCTATG
    GGAGATATTC AGGTTGGAAT GGCTGACAAA AAAGGCCAGT TGGAGGTGGA GGTGATACGA
    GCCCGTGGTC TTGTTGTAAA ACCAGGCTCA AAGACACTGC CAGCACCCTA TGTTAAGGTG
    TATCTCTTAG AAAATGGAGT GTGCATAGCC AAAAAGAAAA CAAAAGTAGC AAGAAAAACG
    CTGGAGCCTC TATACCAGCA AGTGTTAGCG TTTGAGGAAT CACCACAAGG GAAGGTCCTA
    CAGATCATTG TTTGGGGTGA CTATGGACGC ATGGACCACA AGTCCTTCAT GGGGGTGGCT
    CAGATACTTT TAGATGAACT GGACCTATCC AGCATGGTGA TTGGATGGTA TAAACTTTTC
    CCCCCTTCAT CTTTAGTAGA CCCAACGTTG GCTCCACTCA CTAGGAGAGC TTCCCAATCA
    TCTCTTGAAA GCTCCACGGG ACCTTCTTAT GCTCGTTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa59929
    Clone ID Related Accession (Same CDS sequence) XM_031903584.1
    Accession Version XM_031903584.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4059bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 2 isoform X41
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCTGCTC CACTAGGGCC CAGGGGCGGC CCTCTGCCTC CTGCACAGCC ACAGGCTCCA 
    ATGCCAGAGA TGCCGGACCT CAGCCACCTG ACAGAGGATG AGAGGAAAAT CATAATGGCT
    GTGATGGAGA GGCAGAAGAA AGAAGAGGAG AAGGAGCAGT CAGTGTTAAA AAAATTGCAC
    CAGCAGTTTG AAATGTACAA GGAGCAAGTG AAGAAGATGG GCGAGGAAGC ACAGCAGCAG
    CAGGAGCAGA AGGGCGAAGC TCCAACCTGT GGAATCTGCC ACAAAACAAA GTTTGCTGAT
    GGCTGTGGCC ACGACTGTTC GTATTGCCAA ACTAAATTCT GCGCGCGGTG TGGAGGGCGA
    GTGTCTCTGC GCTCAAATAA GGAGGACAAA GTGGTAATGT GGGTATGTAA TTTGTGCCGA
    AAACAACAAG AAATCCTCAC CAAATCAGGG GCCTGGTTTT ACAGCAGTGG GTCAAGCACA
    CCCCTAAAGC CCGGCGAAGA GAGCCAGGCA GGGTACCAAA CGGAAGAAGC ACCTCAGGAG
    AAAAAAGCTA AGCTACAAGA GCAGTCCCAA TACCAGGGAC CCTCAGGGGA CTTATTAACA
    CCAGCCTCGG ACAAAAACCG ACCGCAAGGG ATCGTCCGGC AAGATTCCAT CAAAAATGGT
    TCCGAACTGA AGCACCCGAT TCCTGGCGAC CTCGCATCTG ACAGGAAAAG AAGCCCTTCA
    ATACCTAGAG ACCAGAATCG GAGATATGAT ACAAGGGACG ATATAGGTGA ACATTCACAG
    CATGCTACAT CAGACAGCGC CATGCCTAGA TCACCATCAG ACTACTCGGA CAGGCGATTG
    CAACGAGGGT CTCAGCTCTA TGATGAGCCT GAGCACGGTG ACTACAGAAG AAGTAGGCGG
    CGCTCCAATG AGTATTCGGT AGATGAGGAG GATATCCAAG ACAGGGAGGA ATATGAAAGG
    CAGAGAAGAG AGGAGGAGTA CCAGGCAAGG TACCGGAGTG ACCCCAACTT GGCCAGGTAC
    CCTGTTAAGC CACAACCGTA TGAAGAGCAA ATGCGCATCC ATGCAGAGGT TTCAAGGGCT
    CGACAACGAA GGCACAGCGA TGTGTCTTTA GCTCAGACAG AATTTGAGGA GTCTCGGATG
    TCAACGCTGA GGATGGAGAG ACCTTCAAGG CAAAGATCTG CGTCAGAGCG TAGAGCAGCG
    ATGGAAAGCC AGCGTTCATA TTCAATAGAA AGAACTAGAG ATGTGCAAGG GCCAGGTCCT
    GGTCGGCAAA GGACCTCAAA TCATAGTCCC CCAACACCAA GAAGGAGTCC CATTCCCAAT
    GATAGGCCAG ATATGAGGCG CACAGATTCT TTACGGAAAC AGCACCACTT GGACCCAAGT
    TCTGCCGTAA GGAAAACCAA AAGGGAAAAA ATGGAAACTA TGTTAAGGAA TGATTCTCTT
    AGCTCAGATC AGTCTGAATC TGTCCGGCCC CCACCACCAA AGCCTCATAA AACAAAAAAG
    GGAGGTAAAA TGCGTCAGGT TTCCTTGAGC AGTTCAGAAG AAGAGTTGGC GTCTACGCCG
    GAGTACACGA GCTGCGATGA TGTGGAGATT GAAAGTGAAA GTGTTAGTGA GAAAGGTGAC
    ATGGACTACA ACTGGGTGGA GCATTCGTCT TGGCACAGCA GTGAGGCCTC CCCAATGTCT
    TTGCACCCTG TCACGTGGCA ACCTTCCAAA GATGGAGACC GCTTAATTGG TCGCATATTA
    TTAAACAAGC GGCTCAAAGA TGGGAGCGTC CCGCGAGATT CTGGAGCCAT GCTGGGGCTG
    AAGGTCGTTG GAGGGAAAAT GACAGAATCA GGTCGACTTT GTGCGTTTAT CACCAAAGTG
    AAGAAGGGAA GCCTGGCAGA TACAGTAGGC CATCTTCGAC CAGGTGATGA GGTTTTAGAA
    TGGAGTGGGC GGATTTTGCA AGGAGCGACG TTCGAAGAAG TTTACAATAT TATACTGGAG
    TCCAAACCCG AGGCACAAGT AGAATTATTG GTCTCCAGAC CCATAGGAGA TATACCCCGG
    ATTCCTGACA GCACACACGC ACAACTGGAA TCCAGTTCTA GCTCATTTGA ATCCCAGAAA
    ATGGATCGCC CTTCTATTTC AGTTACTTCC CCCATGAGTC CTGGAATGTT ACGGGATGTC
    CCCCAGTTTC TTTCTGGACA ACTTTCAATT AAACTCTGGT ATGACAAGGT TGGCCACCAG
    TTAATTGTCA CGATATTAGG AGCAAAGGAT CTGCCATCGC GGGAAGATGG GAGGCCGAGA
    AATCCTTACG TAAAAATTTA CTTTCTTCCA GATCGAAGTG ATAAGAACAA AAGGAGAACA
    AAGACTGTGA AGAAAACCGT AGAGCCCAAA TGGAACCAAA CATTCATCTA TTCTCCGGTT
    CATCGGAGGG AATTTCGGGA ACGAATGTTG GAAATAACAC TTTGGGATCA AGCTCGGGTT
    CGAGAAGAAG AGAGTGAATT TTTAGGAGAG ATTCTAATTG AATTAGAAAC TGCACTATTA
    GATGATGAGC CACATTGGTA CAAGCTACAG ACGCATGATG TGTCTTCATT GCCACTTCCA
    CACCCATCCC CGTTCATGCA AAGAAGGCAG CTGCACGGCG AGAGCCCAAC CCGGAGGTTG
    CAAAGATCCA AAAGGATAAG CGACAGCGAA GTGTCTGACT ACGACTGTGA TGATGGCGTT
    GGTGTTGTTT CAGACTTTCG GCATAATGGG CGTGACCTGC AGAGTTCAAC GTTATCTGTT
    CCGGAACAAG TAATGTCGTC CAATCACTGT TCCCGGACTG GGCCCCCGCA TGTAGACAGC
    ATAGGAAGAA CGAGGTCATG GTCTCCCAGT GTCCCTCCTC CGCAAAGTAG GAATTTGGAA
    CAGGGACCGA GAGGGACAAG ATCTGGCCCT GCACATTACA ATACCATGAG CAGAGTGGAA
    AGGCATCGAG TATTAGATAA CCACTACTCC CCAGACAGAG ACAGTCATTA TCTTACTCTA
    CCTCGCACCA GATTCAGTCA GTCCTTTGAC CATCACCTAG CAGAGCCAAG GAATTGTGAA
    GCGGCAGATA GACAGCCATA TCAAAGATCC AGGTCCACTG AACAACGGCC TGTGCTGGAG
    CGGACCAGCT CCCGCTCCCG ATCTACTGAG CGTCCTGACT CAAACCTCAT GAGGTCGATG
    CCTTCATTAA TGACGGGAAG ATCTGCCCCT CCTTCACCCG CCTTATCGAG GGCACACCCT
    CCTCGTACCG GCTCTGTTCA GACAAGTCCT TCAGGCACCC CGTTAGCAGG GCGGAGGGGG
    CGCCAGCTCC CGCAACTTCC CCCAAAAGGC ACGTTAGAAA GAAAAGTCGG AGGGAAGAAG
    CTGAAGAGCA CCATCCAGAG AAGTACGGAA ACAGGCCTGG CCGTGGAAAT GAGAAACTGG
    ATGAGCCGCC AAGCAAGCAG GGAGTCCACA GATGGGAGTA TGAACAGCTA CAGCTCAGAG
    GGGAATCTGA TATTCCCTGG GGTCCGACTG GGTTCCGACA GCCAGTTCAG TGACTTTTTG
    GATGGTCTTG GTCCTGCCCA GCTGGTTGGT CGCCAGACTT TGGCCACGCC CTCTATGGGA
    GATATTCAGG TTGGAATGGC TGACAAAAAA GGCCAGTTGG AGGTGGAGGT GATACGAGCC
    CGTGGTCTTG TTGTAAAACC AGGCTCAAAG ACACTGCCAG CACCCTATGT TAAGGTGTAT
    CTCTTAGAAA ATGGAGTGTG CATAGCCAAA AAGAAAACAA AAGTAGCAAG AAAAACGCTG
    GAGCCTCTAT ACCAGCAAGT GTTAGCGTTT GAGGAATCAC CACAAGGGAA GGTCCTACAG
    ATCATTGTTT GGGGTGACTA TGGACGCATG GACCACAAGT CCTTCATGGG GGTGGCTCAG
    ATACTTTTAG ATGAACTGGA CCTATCCAGC ATGGTGATTG GATGGTATAA ACTTTTCCCC
    CCTTCATCTT TAGTAGACCC AACGTTGGCT CCACTCACTA GGAGAGCTTC CCAATCATCT
    CTTGAAAGCT CCACGGGACC TTCTTATGCT CGTTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031759444.1
    CDS419..4477
    Translation

    Target ORF information:

    RefSeq Version XM_031903584.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 2 (rims2), transcript variant X41, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031903584.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCTGCTC CACTAGGGCC CAGGGGCGGC CCTCTGCCTC CTGCACAGCC ACAGGCTCCA 
    ATGCCAGAGA TGCCGGACCT CAGCCACCTG ACAGAGGATG AGAGGAAAAT CATAATGGCT
    GTGATGGAGA GGCAGAAGAA AGAAGAGGAG AAGGAGCAGT CAGTGTTAAA AAAATTGCAC
    CAGCAGTTTG AAATGTACAA GGAGCAAGTG AAGAAGATGG GCGAGGAAGC ACAGCAGCAG
    CAGGAGCAGA AGGGCGAAGC TCCAACCTGT GGAATCTGCC ACAAAACAAA GTTTGCTGAT
    GGCTGTGGCC ACGACTGTTC GTATTGCCAA ACTAAATTCT GCGCGCGGTG TGGAGGGCGA
    GTGTCTCTGC GCTCAAATAA GGAGGACAAA GTGGTAATGT GGGTATGTAA TTTGTGCCGA
    AAACAACAAG AAATCCTCAC CAAATCAGGG GCCTGGTTTT ACAGCAGTGG GTCAAGCACA
    CCCCTAAAGC CCGGCGAAGA GAGCCAGGCA GGGTACCAAA CGGAAGAAGC ACCTCAGGAG
    AAAAAAGCTA AGCTACAAGA GCAGTCCCAA TACCAGGGAC CCTCAGGGGA CTTATTAACA
    CCAGCCTCGG ACAAAAACCG ACCGCAAGGG ATCGTCCGGC AAGATTCCAT CAAAAATGGT
    TCCGAACTGA AGCACCCGAT TCCTGGCGAC CTCGCATCTG ACAGGAAAAG AAGCCCTTCA
    ATACCTAGAG ACCAGAATCG GAGATATGAT ACAAGGGACG ATATAGGTGA ACATTCACAG
    CATGCTACAT CAGACAGCGC CATGCCTAGA TCACCATCAG ACTACTCGGA CAGGCGATTG
    CAACGAGGGT CTCAGCTCTA TGATGAGCCT GAGCACGGTG ACTACAGAAG AAGTAGGCGG
    CGCTCCAATG AGTATTCGGT AGATGAGGAG GATATCCAAG ACAGGGAGGA ATATGAAAGG
    CAGAGAAGAG AGGAGGAGTA CCAGGCAAGG TACCGGAGTG ACCCCAACTT GGCCAGGTAC
    CCTGTTAAGC CACAACCGTA TGAAGAGCAA ATGCGCATCC ATGCAGAGGT TTCAAGGGCT
    CGACAACGAA GGCACAGCGA TGTGTCTTTA GCTCAGACAG AATTTGAGGA GTCTCGGATG
    TCAACGCTGA GGATGGAGAG ACCTTCAAGG CAAAGATCTG CGTCAGAGCG TAGAGCAGCG
    ATGGAAAGCC AGCGTTCATA TTCAATAGAA AGAACTAGAG ATGTGCAAGG GCCAGGTCCT
    GGTCGGCAAA GGACCTCAAA TCATAGTCCC CCAACACCAA GAAGGAGTCC CATTCCCAAT
    GATAGGCCAG ATATGAGGCG CACAGATTCT TTACGGAAAC AGCACCACTT GGACCCAAGT
    TCTGCCGTAA GGAAAACCAA AAGGGAAAAA ATGGAAACTA TGTTAAGGAA TGATTCTCTT
    AGCTCAGATC AGTCTGAATC TGTCCGGCCC CCACCACCAA AGCCTCATAA AACAAAAAAG
    GGAGGTAAAA TGCGTCAGGT TTCCTTGAGC AGTTCAGAAG AAGAGTTGGC GTCTACGCCG
    GAGTACACGA GCTGCGATGA TGTGGAGATT GAAAGTGAAA GTGTTAGTGA GAAAGGTGAC
    ATGGACTACA ACTGGGTGGA GCATTCGTCT TGGCACAGCA GTGAGGCCTC CCCAATGTCT
    TTGCACCCTG TCACGTGGCA ACCTTCCAAA GATGGAGACC GCTTAATTGG TCGCATATTA
    TTAAACAAGC GGCTCAAAGA TGGGAGCGTC CCGCGAGATT CTGGAGCCAT GCTGGGGCTG
    AAGGTCGTTG GAGGGAAAAT GACAGAATCA GGTCGACTTT GTGCGTTTAT CACCAAAGTG
    AAGAAGGGAA GCCTGGCAGA TACAGTAGGC CATCTTCGAC CAGGTGATGA GGTTTTAGAA
    TGGAGTGGGC GGATTTTGCA AGGAGCGACG TTCGAAGAAG TTTACAATAT TATACTGGAG
    TCCAAACCCG AGGCACAAGT AGAATTATTG GTCTCCAGAC CCATAGGAGA TATACCCCGG
    ATTCCTGACA GCACACACGC ACAACTGGAA TCCAGTTCTA GCTCATTTGA ATCCCAGAAA
    ATGGATCGCC CTTCTATTTC AGTTACTTCC CCCATGAGTC CTGGAATGTT ACGGGATGTC
    CCCCAGTTTC TTTCTGGACA ACTTTCAATT AAACTCTGGT ATGACAAGGT TGGCCACCAG
    TTAATTGTCA CGATATTAGG AGCAAAGGAT CTGCCATCGC GGGAAGATGG GAGGCCGAGA
    AATCCTTACG TAAAAATTTA CTTTCTTCCA GATCGAAGTG ATAAGAACAA AAGGAGAACA
    AAGACTGTGA AGAAAACCGT AGAGCCCAAA TGGAACCAAA CATTCATCTA TTCTCCGGTT
    CATCGGAGGG AATTTCGGGA ACGAATGTTG GAAATAACAC TTTGGGATCA AGCTCGGGTT
    CGAGAAGAAG AGAGTGAATT TTTAGGAGAG ATTCTAATTG AATTAGAAAC TGCACTATTA
    GATGATGAGC CACATTGGTA CAAGCTACAG ACGCATGATG TGTCTTCATT GCCACTTCCA
    CACCCATCCC CGTTCATGCA AAGAAGGCAG CTGCACGGCG AGAGCCCAAC CCGGAGGTTG
    CAAAGATCCA AAAGGATAAG CGACAGCGAA GTGTCTGACT ACGACTGTGA TGATGGCGTT
    GGTGTTGTTT CAGACTTTCG GCATAATGGG CGTGACCTGC AGAGTTCAAC GTTATCTGTT
    CCGGAACAAG TAATGTCGTC CAATCACTGT TCCCGGACTG GGCCCCCGCA TGTAGACAGC
    ATAGGAAGAA CGAGGTCATG GTCTCCCAGT GTCCCTCCTC CGCAAAGTAG GAATTTGGAA
    CAGGGACCGA GAGGGACAAG ATCTGGCCCT GCACATTACA ATACCATGAG CAGAGTGGAA
    AGGCATCGAG TATTAGATAA CCACTACTCC CCAGACAGAG ACAGTCATTA TCTTACTCTA
    CCTCGCACCA GATTCAGTCA GTCCTTTGAC CATCACCTAG CAGAGCCAAG GAATTGTGAA
    GCGGCAGATA GACAGCCATA TCAAAGATCC AGGTCCACTG AACAACGGCC TGTGCTGGAG
    CGGACCAGCT CCCGCTCCCG ATCTACTGAG CGTCCTGACT CAAACCTCAT GAGGTCGATG
    CCTTCATTAA TGACGGGAAG ATCTGCCCCT CCTTCACCCG CCTTATCGAG GGCACACCCT
    CCTCGTACCG GCTCTGTTCA GACAAGTCCT TCAGGCACCC CGTTAGCAGG GCGGAGGGGG
    CGCCAGCTCC CGCAACTTCC CCCAAAAGGC ACGTTAGAAA GAAAAGTCGG AGGGAAGAAG
    CTGAAGAGCA CCATCCAGAG AAGTACGGAA ACAGGCCTGG CCGTGGAAAT GAGAAACTGG
    ATGAGCCGCC AAGCAAGCAG GGAGTCCACA GATGGGAGTA TGAACAGCTA CAGCTCAGAG
    GGGAATCTGA TATTCCCTGG GGTCCGACTG GGTTCCGACA GCCAGTTCAG TGACTTTTTG
    GATGGTCTTG GTCCTGCCCA GCTGGTTGGT CGCCAGACTT TGGCCACGCC CTCTATGGGA
    GATATTCAGG TTGGAATGGC TGACAAAAAA GGCCAGTTGG AGGTGGAGGT GATACGAGCC
    CGTGGTCTTG TTGTAAAACC AGGCTCAAAG ACACTGCCAG CACCCTATGT TAAGGTGTAT
    CTCTTAGAAA ATGGAGTGTG CATAGCCAAA AAGAAAACAA AAGTAGCAAG AAAAACGCTG
    GAGCCTCTAT ACCAGCAAGT GTTAGCGTTT GAGGAATCAC CACAAGGGAA GGTCCTACAG
    ATCATTGTTT GGGGTGACTA TGGACGCATG GACCACAAGT CCTTCATGGG GGTGGCTCAG
    ATACTTTTAG ATGAACTGGA CCTATCCAGC ATGGTGATTG GATGGTATAA ACTTTTCCCC
    CCTTCATCTT TAGTAGACCC AACGTTGGCT CCACTCACTA GGAGAGCTTC CCAATCATCT
    CTTGAAAGCT CCACGGGACC TTCTTATGCT CGTTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa59930
    Clone ID Related Accession (Same CDS sequence) XM_031903585.1
    Accession Version XM_031903585.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3819bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 2 isoform X42
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCAGTTCG AGACGCTGCG CCAGTTTTGC AACACGGTCC TGTCGCATTT CCACGAAGCT 
    TTCCCCACTC CGCCGAACAT ACTGCAGAAT GATCTGTTCG GGCAGCCAGG GAACAGCCCC
    GGGAAAAGAA GCCCTTCAAT ACCTAGAGAC CAGAATCGGA GATATGATAC AAGGGACGAT
    ATAGGTGAAC ATTCACAGCA TGCTACATCA GACAGCGCCA TGCCTAGATC ACCATCAGAC
    TACTCGGACA GGCGATTGCA ACGAGGGTCT CAGCTCTATG ATGAGCCTGA GCACGGTGAC
    TACAGAAGAA GTAGGCGGCG CTCCAATGAG TATTCGGTAG ATGAGGAGGA TATCCAAGAC
    AGGGAGGAAT ATGAAAGGCA GAGAAGAGAG GAGGAGTACC AGGCAAGGTA CCGGAGTGAC
    CCCAACTTGG CCAGGTACCC TGTTAAGCCA CAACCGTATG AAGAGCAAAT GCGCATCCAT
    GCAGAGGTTT CAAGGGCTCG ACAACGAAGG CACAGCGATG TGTCTTTAGC TCAGACAGAA
    TTTGAGGAGT CTCGGATGTC AACGCTGAGG ATGGAGAGAC CTTCAAGGCA AAGATCTGCG
    TCAGAGCGTA GAGCAGCGAT GGAAAGCCAG CGTTCATATT CAATAGAAAG AACTAGAGAT
    GTGCAAGGGC CAGGTCCTGG TCGGCAAAGG ACCTCAAATC ATAGTCCCCC AACACCAAGA
    AGGAGTCCCA TTCCCAATGA TAGGCCAGAT ATGAGGCGCA CAGATTCTTT ACGGAAACAG
    CACCACTTGG ACCCAAGTTC TGCCGTAAGG AAAACCAAAA GGGAAAAAAT GGAAACTATG
    TTAAGGAATG ATTCTCTTAG CTCAGATCAG TCTGAATCTG TCCGGCCCCC ACCACCAAAG
    CCTCATAAAA CAAAAAAGGG AGGTAAAATG CGTCAGGTTT CCTTGAGCAG TTCAGAAGAA
    GAGTTGGCGT CTACGCCGGA GTACACGAGC TGCGATGATG TGGAGATTGA AAGTGAAAGT
    GTTAGTGAGA AAGGTGACAT GGACTACAAC TGGGTGGAGC ATTCGTCTTG GCACAGCAGT
    GAGGCCTCCC CAATGTCTTT GCACCCTGTC ACGTGGCAAC CTTCCAAAGA TGGAGACCGC
    TTAATTGGTC GCATATTATT AAACAAGCGG CTCAAAGATG GGAGCGTCCC GCGAGATTCT
    GGAGCCATGC TGGGGCTGAA GGTCGTTGGA GGGAAAATGA CAGAATCAGG TCGACTTTGT
    GCGTTTATCA CCAAAGTGAA GAAGGGAAGC CTGGCAGATA CAGTAGGCCA TCTTCGACCA
    GGTGATGAGG TTTTAGAATG GAGTGGGCGG ATTTTGCAAG GAGCGACGTT CGAAGAAGTT
    TACAATATTA TACTGGAGTC CAAACCCGAG GCACAAGTAG AATTATTGGT CTCCAGACCC
    ATAGGAGATA TACCCCGGAT TCCTGACAGC ACACACGCAC AACTGGAATC CAGTTCTAGC
    TCATTTGAAT CCCAGAAAAT GGATCGCCCT TCTATTTCAG TTACTTCCCC CATGAGTCCT
    GGAATGTTAC GGGATGTCCC CCAGTTTCTT TCTGGACAAC TTTCAATTAA ACTCTGGTAT
    GACAAGGTTG GCCACCAGTT AATTGTCACG ATATTAGGAG CAAAGGATCT GCCATCGCGG
    GAAGATGGGA GGCCGAGAAA TCCTTACGTA AAAATTTACT TTCTTCCAGA TCGAAGTGAT
    AAGAACAAAA GGAGAACAAA GACTGTGAAG AAAACCGTAG AGCCCAAATG GAACCAAACA
    TTCATCTATT CTCCGGTTCA TCGGAGGGAA TTTCGGGAAC GAATGTTGGA AATAACACTT
    TGGGATCAAG CTCGGGTTCG AGAAGAAGAG AGTGAATTTT TAGGAGAGAT TCTAATTGAA
    TTAGAAACTG CACTATTAGA TGATGAGCCA CATTGGTACA AGCTACAGAC GCATGATGTG
    TCTTCATTGC CACTTCCACA CCCATCCCCG TTCATGCAAA GAAGGCAGCT GCACGGCGAG
    AGCCCAACCC GGAGGTTGCA AAGATCCAAA AGGATAAGCG ACAGCGAAGT GTCTGACTAC
    GACTGTGATG ATGGCGTTGG TGTTGTTTCA GACTTTCGGC ATAATGGGCG TGACCTGCAG
    AGTTCAACGT TATCTGTTCC GGAACAAGTA ATGTCGTCCA ATCACTGTTC CCGGACTGGG
    CCCCCGCATG TAGACAGCAT AGGAAGAACG AGGTCATGGT CTCCCAGTGT CCCTCCTCCG
    CAAAGTAGGA ATTTGGAACA GGGACCGAGA GGGACAAGAT CTGGCCCTGC ACATTACAAT
    ACCATGAGCA GAGTGGAAAG GCATCGAGTA TTAGATAACC ACTACTCCCC AGACAGAGAC
    AGGGCACACC CTCCTCGTAC CGGCTCTGTT CAGACAAGTC CTTCAGGCAC CCCGTTAGCA
    GGGCGGAGGG GGCGCCAGCT CCCGCAACTT CCCCCAAAAG GCACGTTAGA AAGAAAAAAT
    GGAGACAAGG AAATAGAGCT TTATGAAGAA GATATGGACT CAACGCGGAG GCGAAACTCA
    GGTGCTATGG ACGTAGAAGA AAGGACGCGA CAGCTGAAAG TGAAGATCAA CAAATACAAG
    CAGGTGGGAG GATCAGATTC TCGGCTAGAG AAGGATTATC ATCCAAAGTA TCGGACGGGG
    CGGGATCCGC AGAGGGGCTC AGACAATATG TCCAACAAGT CATCAGACAG CGATGTTAGT
    GATGTGTCTG CCGTATCACG GACCAGCAGC GCCTCTCGTT TTAGTAGTAC GAGTTATATG
    TCTGTGCAGT CGGAAAGGCC TCGAGGGAAC ATGAAAATAG GTGCATTGGC ATCCAAAATG
    CAAAGCAGGC AAATGGGCGC CTCAGGGAAG AACATGACCA AAAGTACCAG CATCGGAGGG
    GATATGTACA CCCTGGAGAA GAACGACGGA AGCCAGTCTG ACACAGCCGT GGGCACTGTG
    GGTGCGGGTG GAAAGAAGAG ACGTTCAAGC ATAGGCGCCA AAATGGTGGC CATTGTGGGC
    TTGTCACGAA AAAGCCGAAG TACCTCACAG CTCAGCCAAA CAGAAGTCGG AGGGAAGAAG
    CTGAAGAGCA CCATCCAGAG AAGTACGGAA ACAGGCCTGG CCGTGGAAAT GAGAAACTGG
    ATGAGCCGCC AAGCAAGCAG GGAGTCCACA GATGGGAGTA TGAACAGCTA CAGCTCAGAG
    GGGAATCTGA TATTCCCTGG GGTCCGACTG GGTTCCGACA GCCAGTTCAG TGACTTTTTG
    GATGGTCTTG GTCCTGCCCA GCTGGTTGGT CGCCAGACTT TGGCCACGCC CTCTATGGGA
    GATATTCAGG TTGGAATGGC TGACAAAAAA GGCCAGTTGG AGGTGGAGGT GATACGAGCC
    CGTGGTCTTG TTGTAAAACC AGGCTCAAAG ACACTGCCAG CACCCTATGT TAAGGTGTAT
    CTCTTAGAAA ATGGAGTGTG CATAGCCAAA AAGAAAACAA AAGTAGCAAG AAAAACGCTG
    GAGCCTCTAT ACCAGCAAGT GTTAGCGTTT GAGGAATCAC CACAAGGGAA GGTCCTACAG
    ATCATTGTTT GGGGTGACTA TGGACGCATG GACCACAAGT CCTTCATGGG GGTGGCTCAG
    ATACTTTTAG ATGAACTGGA CCTATCCAGC ATGGTGATTG GATGGTATAA ACTTTTCCCC
    CCTTCATCTT TAGTAGACCC AACGTTGGCT CCACTCACTA GGAGAGCTTC CCAATCATCT
    CTTGAAAGCT CCACGGGACC TTCTTATGCT CGTTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031759445.1
    CDS302..4120
    Translation

    Target ORF information:

    RefSeq Version XM_031903585.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 2 (rims2), transcript variant X42, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031903585.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCAGTTCG AGACGCTGCG CCAGTTTTGC AACACGGTCC TGTCGCATTT CCACGAAGCT 
    TTCCCCACTC CGCCGAACAT ACTGCAGAAT GATCTGTTCG GGCAGCCAGG GAACAGCCCC
    GGGAAAAGAA GCCCTTCAAT ACCTAGAGAC CAGAATCGGA GATATGATAC AAGGGACGAT
    ATAGGTGAAC ATTCACAGCA TGCTACATCA GACAGCGCCA TGCCTAGATC ACCATCAGAC
    TACTCGGACA GGCGATTGCA ACGAGGGTCT CAGCTCTATG ATGAGCCTGA GCACGGTGAC
    TACAGAAGAA GTAGGCGGCG CTCCAATGAG TATTCGGTAG ATGAGGAGGA TATCCAAGAC
    AGGGAGGAAT ATGAAAGGCA GAGAAGAGAG GAGGAGTACC AGGCAAGGTA CCGGAGTGAC
    CCCAACTTGG CCAGGTACCC TGTTAAGCCA CAACCGTATG AAGAGCAAAT GCGCATCCAT
    GCAGAGGTTT CAAGGGCTCG ACAACGAAGG CACAGCGATG TGTCTTTAGC TCAGACAGAA
    TTTGAGGAGT CTCGGATGTC AACGCTGAGG ATGGAGAGAC CTTCAAGGCA AAGATCTGCG
    TCAGAGCGTA GAGCAGCGAT GGAAAGCCAG CGTTCATATT CAATAGAAAG AACTAGAGAT
    GTGCAAGGGC CAGGTCCTGG TCGGCAAAGG ACCTCAAATC ATAGTCCCCC AACACCAAGA
    AGGAGTCCCA TTCCCAATGA TAGGCCAGAT ATGAGGCGCA CAGATTCTTT ACGGAAACAG
    CACCACTTGG ACCCAAGTTC TGCCGTAAGG AAAACCAAAA GGGAAAAAAT GGAAACTATG
    TTAAGGAATG ATTCTCTTAG CTCAGATCAG TCTGAATCTG TCCGGCCCCC ACCACCAAAG
    CCTCATAAAA CAAAAAAGGG AGGTAAAATG CGTCAGGTTT CCTTGAGCAG TTCAGAAGAA
    GAGTTGGCGT CTACGCCGGA GTACACGAGC TGCGATGATG TGGAGATTGA AAGTGAAAGT
    GTTAGTGAGA AAGGTGACAT GGACTACAAC TGGGTGGAGC ATTCGTCTTG GCACAGCAGT
    GAGGCCTCCC CAATGTCTTT GCACCCTGTC ACGTGGCAAC CTTCCAAAGA TGGAGACCGC
    TTAATTGGTC GCATATTATT AAACAAGCGG CTCAAAGATG GGAGCGTCCC GCGAGATTCT
    GGAGCCATGC TGGGGCTGAA GGTCGTTGGA GGGAAAATGA CAGAATCAGG TCGACTTTGT
    GCGTTTATCA CCAAAGTGAA GAAGGGAAGC CTGGCAGATA CAGTAGGCCA TCTTCGACCA
    GGTGATGAGG TTTTAGAATG GAGTGGGCGG ATTTTGCAAG GAGCGACGTT CGAAGAAGTT
    TACAATATTA TACTGGAGTC CAAACCCGAG GCACAAGTAG AATTATTGGT CTCCAGACCC
    ATAGGAGATA TACCCCGGAT TCCTGACAGC ACACACGCAC AACTGGAATC CAGTTCTAGC
    TCATTTGAAT CCCAGAAAAT GGATCGCCCT TCTATTTCAG TTACTTCCCC CATGAGTCCT
    GGAATGTTAC GGGATGTCCC CCAGTTTCTT TCTGGACAAC TTTCAATTAA ACTCTGGTAT
    GACAAGGTTG GCCACCAGTT AATTGTCACG ATATTAGGAG CAAAGGATCT GCCATCGCGG
    GAAGATGGGA GGCCGAGAAA TCCTTACGTA AAAATTTACT TTCTTCCAGA TCGAAGTGAT
    AAGAACAAAA GGAGAACAAA GACTGTGAAG AAAACCGTAG AGCCCAAATG GAACCAAACA
    TTCATCTATT CTCCGGTTCA TCGGAGGGAA TTTCGGGAAC GAATGTTGGA AATAACACTT
    TGGGATCAAG CTCGGGTTCG AGAAGAAGAG AGTGAATTTT TAGGAGAGAT TCTAATTGAA
    TTAGAAACTG CACTATTAGA TGATGAGCCA CATTGGTACA AGCTACAGAC GCATGATGTG
    TCTTCATTGC CACTTCCACA CCCATCCCCG TTCATGCAAA GAAGGCAGCT GCACGGCGAG
    AGCCCAACCC GGAGGTTGCA AAGATCCAAA AGGATAAGCG ACAGCGAAGT GTCTGACTAC
    GACTGTGATG ATGGCGTTGG TGTTGTTTCA GACTTTCGGC ATAATGGGCG TGACCTGCAG
    AGTTCAACGT TATCTGTTCC GGAACAAGTA ATGTCGTCCA ATCACTGTTC CCGGACTGGG
    CCCCCGCATG TAGACAGCAT AGGAAGAACG AGGTCATGGT CTCCCAGTGT CCCTCCTCCG
    CAAAGTAGGA ATTTGGAACA GGGACCGAGA GGGACAAGAT CTGGCCCTGC ACATTACAAT
    ACCATGAGCA GAGTGGAAAG GCATCGAGTA TTAGATAACC ACTACTCCCC AGACAGAGAC
    AGGGCACACC CTCCTCGTAC CGGCTCTGTT CAGACAAGTC CTTCAGGCAC CCCGTTAGCA
    GGGCGGAGGG GGCGCCAGCT CCCGCAACTT CCCCCAAAAG GCACGTTAGA AAGAAAAAAT
    GGAGACAAGG AAATAGAGCT TTATGAAGAA GATATGGACT CAACGCGGAG GCGAAACTCA
    GGTGCTATGG ACGTAGAAGA AAGGACGCGA CAGCTGAAAG TGAAGATCAA CAAATACAAG
    CAGGTGGGAG GATCAGATTC TCGGCTAGAG AAGGATTATC ATCCAAAGTA TCGGACGGGG
    CGGGATCCGC AGAGGGGCTC AGACAATATG TCCAACAAGT CATCAGACAG CGATGTTAGT
    GATGTGTCTG CCGTATCACG GACCAGCAGC GCCTCTCGTT TTAGTAGTAC GAGTTATATG
    TCTGTGCAGT CGGAAAGGCC TCGAGGGAAC ATGAAAATAG GTGCATTGGC ATCCAAAATG
    CAAAGCAGGC AAATGGGCGC CTCAGGGAAG AACATGACCA AAAGTACCAG CATCGGAGGG
    GATATGTACA CCCTGGAGAA GAACGACGGA AGCCAGTCTG ACACAGCCGT GGGCACTGTG
    GGTGCGGGTG GAAAGAAGAG ACGTTCAAGC ATAGGCGCCA AAATGGTGGC CATTGTGGGC
    TTGTCACGAA AAAGCCGAAG TACCTCACAG CTCAGCCAAA CAGAAGTCGG AGGGAAGAAG
    CTGAAGAGCA CCATCCAGAG AAGTACGGAA ACAGGCCTGG CCGTGGAAAT GAGAAACTGG
    ATGAGCCGCC AAGCAAGCAG GGAGTCCACA GATGGGAGTA TGAACAGCTA CAGCTCAGAG
    GGGAATCTGA TATTCCCTGG GGTCCGACTG GGTTCCGACA GCCAGTTCAG TGACTTTTTG
    GATGGTCTTG GTCCTGCCCA GCTGGTTGGT CGCCAGACTT TGGCCACGCC CTCTATGGGA
    GATATTCAGG TTGGAATGGC TGACAAAAAA GGCCAGTTGG AGGTGGAGGT GATACGAGCC
    CGTGGTCTTG TTGTAAAACC AGGCTCAAAG ACACTGCCAG CACCCTATGT TAAGGTGTAT
    CTCTTAGAAA ATGGAGTGTG CATAGCCAAA AAGAAAACAA AAGTAGCAAG AAAAACGCTG
    GAGCCTCTAT ACCAGCAAGT GTTAGCGTTT GAGGAATCAC CACAAGGGAA GGTCCTACAG
    ATCATTGTTT GGGGTGACTA TGGACGCATG GACCACAAGT CCTTCATGGG GGTGGCTCAG
    ATACTTTTAG ATGAACTGGA CCTATCCAGC ATGGTGATTG GATGGTATAA ACTTTTCCCC
    CCTTCATCTT TAGTAGACCC AACGTTGGCT CCACTCACTA GGAGAGCTTC CCAATCATCT
    CTTGAAAGCT CCACGGGACC TTCTTATGCT CGTTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa59897
    Clone ID Related Accession (Same CDS sequence) XM_031903556.1
    Accession Version XM_031903556.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5199bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTCTGCTC CACTAGGGCC CAGGGGCGGC CCTCTGCCTC CTGCACAGCC ACAGGCTCCA 
    ATGCCAGAGA TGCCGGACCT CAGCCACCTG ACAGAGGATG AGAGGAAAAT CATAATGGCT
    GTGATGGAGA GGCAGAAGAA AGAAGAGGAG AAGGAGCAGT CAGTGTTAAA GGTCAAAGAA
    GAACAAAAAG CCCAGCCGGC ACAGTGGTTT CCCTTTAGTG GGATCACTGA GCTGGTTAAT
    AACGTTCTTC AGCCACAGCC AAAAGAGCAA AATGAAAAGG AGCCGGAGAT AAAATTGCAC
    CAGCAGTTTG AAATGTACAA GGAGCAAGTG AAGAAGATGG GCGAGGAAGC ACAGCAGCAG
    CAGGAGCAGA AGGGCGAAGC TCCAACCTGT GGAATCTGCC ACAAAACAAA GTTTGCTGAT
    GGCTGTGGCC ACGACTGTTC GTATTGCCAA ACTAAATTCT GCGCGCGGTG TGGAGGGCGA
    GTGTCTCTGC GCTCAAATAA GGAGGACAAA GTGGTAATGT GGGTATGTAA TTTGTGCCGA
    AAACAACAAG AAATCCTCAC CAAATCAGGG GCCTGGTTTT ACAGCAGTGG GTCAAGCACA
    CCCCTAAAGC CCGGCGAAGA GAGCCAGGCA GGGTACCAAA CGGAAGAAGC ACCTCAGGAG
    AAAAAAGCTA AGCTACAAGA GCAGTCCCAA TACCAGGGAC CCTCAGGGGA CTTATTAACA
    CCAGCCTCGG ACAAAAACCG ACCGCAAGGG ATCGTCCGGC AAGATTCCAT CAAAAATGGT
    TCCGAACTGA AGCACCCGAT TCCTGGCGAC CTCGCATCTG ACAGGAAAAG AAGCCCTTCA
    ATACCTAGAG ACCAGAATCG GAGATATGAT ACAAGGGACG ATATAGGTGA ACATTCACAG
    CATGCTACAT CAGACAGCGC CATGCCTAGA TCACCATCAG ACTACTCGGA CAGGCGATTG
    CAACGAGGGT CTCAGCTCTA TGATGAGCCT GAGCACGGTG ACTACAGAAG AAGTAGGCGG
    CGCTCCAATG AGTATTCGGT AGATGAGGAG GATATCCAAG ACAGGGAGGA ATATGAAAGG
    CAGAGAAGAG AGGAGGAGTA CCAGGCAAGG TACCGGAGTG ACCCCAACTT GGCCAGGTAC
    CCTGTTAAGC CACAACCGTA TGAAGAGCAA ATGCGCATCC ATGCAGAGGT TTCAAGGGCT
    CGACAACGAA GGCACAGCGA TGTGTCTTTA GCTCAGACAG AATTTGAGGA GTCTCGGATG
    TCAACGCTGA GGATGGAGAG ACCTTCAAGG CAAAGATCTG CGTCAGAGCG TAGAGCAGCG
    ATGGAAAGCC AGCGTTCATA TTCAATAGAA AGAACTAGAG ATGTGCAAGG GCCAGGTCCT
    GGTCGGCAAA GGACCTCAAA TCATAGTCCC CCAACACCAA GAAGGAGTCC CATTCCCAAT
    GATAGGCCAG ATATGAGGCG CACAGATTCT TTACGGAAAC AGCACCACTT GGACCCAAGT
    TCTGCCGTAA GGAAAACCAA AAGGGAAAAA ATGGAAACTA TGTTAAGGAA TGATTCTCTT
    AGCTCAGATC AGTCTGAATC TGTCCGGCCC CCACCACCAA AGCCTCATAA AACAAAAAAG
    GGAGGTAAAA TGCGTCAGGT TTCCTTGAGC AGTTCAGAAG AAGAGTTGGC GTCTACGCCG
    GAGTACACGA GCTGCGATGA TGTGGAGATT GAAAGTGAAA GTGTTAGTGA GAAAGGGGAC
    AGTCAAAGGG GAAAAAGAAA AACTAGTGAG CAGGCAGTTT TGTCGGATTC TAACATCTTG
    TCTGAGAGAC AAAAGAAAAT GGTGTGCTTT GGAGGCCACT CTTTGGAAGA GGATTTGGAA
    TGGTCTGAGC CTCAGATTAA AGACTCTGGG GTAGACACGT GTAGTAGCAC CACCCTTAAC
    GAAGAGCATA GCCATAGCGA GAAGCACCCT GTCACGTGGC AACCTTCCAA AGATGGAGAC
    CGCTTAATTG GTCGCATATT ATTAAACAAG CGGCTCAAAG ATGGGAGCGT CCCGCGAGAT
    TCTGGAGCCA TGCTGGGGCT GAAGGTCGTT GGAGGGAAAA TGACAGAATC AGGTCGACTT
    TGTGCGTTTA TCACCAAAGT GAAGAAGGGA AGCCTGGCAG ATACAGTAGG CCATCTTCGA
    CCAGGTGATG AGGTTTTAGA ATGGAGTGGG CGGATTTTGC AAGGAGCGAC GTTCGAAGAA
    GTTTACAATA TTATACTGGA GTCCAAACCC GAGGCACAAG TAGAATTATT GGTCTCCAGA
    CCCATAGGAG ATATACCCCG GATTCCTGAC AGCACACACG CACAACTGGA ATCCAGTTCT
    AGCTCATTTG AATCCCAGAA AATGGATCGC CCTTCTATTT CAGTTACTTC CCCCATGAGT
    CCTGGAATGT TACGGGATGT CCCCCAGTTT CTTTCTGGAC AACTTTCAAG CCAAAGCCTT
    AATAGAAGAA CAGCGCCTTT TGTTCCTAGG GTTCAGATTA AACTCTGGTA TGACAAGGTT
    GGCCACCAGT TAATTGTCAC GATATTAGGA GCAAAGGATC TGCCATCGCG GGAAGATGGG
    AGGCCGAGAA ATCCTTACGT AAAAATTTAC TTTCTTCCAG ATCGAAGTGA TAAGAACAAA
    AGGAGAACAA AGACTGTGAA GAAAACCGTA GAGCCCAAAT GGAACCAAAC ATTCATCTAT
    TCTCCGGTTC ATCGGAGGGA ATTTCGGGAA CGAATGTTGG AAATAACACT TTGGGATCAA
    GCTCGGGTTC GAGAAGAAGA GAGTGAATTT TTAGGAGAGA TTCTAATTGA ATTAGAAACT
    GCACTATTAG ATGATGAGCC ACATTGGTAC AAGCTACAGA CGCATGATGT GTCTTCATTG
    CCACTTCCAC ACCCATCCCC GTTCATGCAA AGAAGGCAGC TGCACGGCGA GAGCCCAACC
    CGGAGGTTGC AAAATAAAGG CTCATATTCA TATAATTCAG GATCCAAAAG GATAAGCGAC
    AGCGAAGTGT CTGACTACGA CTGTGATGAT GGCGTTGGTG TTGTTTCAGA CTTTCGGCAT
    AATGGGCGTG ACCTGCAGAG TTCAACGTTA TCTGTTCCGG AACAAGTAAT GTCGTCCAAT
    CACTGTTCCC GGACTGGGCC CCCGCATGTA GACAGCATAG GAAGAACGAG GTCATGGTCT
    CCCAGTGTCC CTCCTCCGCA AAGTAGGAAT TTGGAACAGG GACCGAGAGG GACAAGATCT
    GGCCCTGCAC ATTACAATAC CATGAGCAGA GTGGAAAGGC ATCGAGTATT AGATAACCAC
    TACTCCCCAG ACAGAGACAG GAATTGTGAA GCGGCAGATA GACAGCCATA TCAAAGATCC
    AGGTCCACTG AACAACGGCC TGTGCTGGAG CGGACCAGCT CCCGCTCCCG ATCTACTGAG
    CGTCCTGACT CAAACCTCAT GAGGTCGATG CCTTCATTAA TGACGGGAAG ATCTGCCCCT
    CCTTCACCCG CCTTATCGAG GGCACACCCT CCTCGTACCG GCTCTGTTCA GACAAGTCCT
    TCAGGCACCC CGTTAGCAGG GCGGAGGGGG CGCCAGCTCC CGCAACTTCC CCCAAAAGGC
    ACGTTAGAAA GAAAAGATAT GGACTCAACG CGGAGGCGAA ACTCAGAGGA AAAGGCTGCA
    GAACAAGAGA ATGGGAACCC AGGTGCTATG GACGTAGAAG AAAGGACGCG ACAGCTGAAA
    GTGAAGATCA ACAAATACAA GCAGGTGGGA GGATCAGATT CTCGGCTAGA GAAGGATTAT
    CATCCAAAGT ATCGGACGGG GCGGGATCCG CAGAGGGGCT CAGACAATAT GTCCAACAAG
    TCATCAGACA GCGATGTTAG TGATGTGTCT GCCGTATCAC GGACCAGCAG CGCCTCTCGT
    TTTAGTAGTA CGAGTTATAT GTCTGTGCAG TCGGAAAGGC CTCGAGGGAA CATGAAAATA
    GGTAGAAGGA CAAGGGAAGT AGAGGAGAGA ATAGAGAGGG CTGAGGGAGG GCAGCATCAC
    AAAGAAATAG GTTTTCTGGA GGAAGAGGAG GACGTTAGGC AGGAAGTAGA TAGTGAGAAG
    GAAACAGAAG TGCAGCATAT AATTGACAAC TGCATGAAGG GGCCAAGCAA AGAAAGTGTA
    GAGGAAGAGA GTCAACAGAA TGAGAAAGAT GAGGAAAGCC ATGATGTTGA GGAAGCCCAT
    AATGAGAATC TGCCAAGGAG GTCCTCAGAA AATGATATAA GTGCATTGGC ATCCAAAATG
    CAAAGCAGGC AAATGGGCGC CTCAGGGAAG AACATGACCA AAAGTACCAG CATCGGAGGG
    GATATGTACA CCCTGGAGAA GAACGACGGA AGCCAGTCTG ACACAGCCGT GGGCACTGTG
    GGTGCGGGTG GAAAGAAGAG ACGTTCAAGC ATAGGCGCCA AAATGGTGGC CATTGTGGGC
    TTGTCACGAA AAAGCCGAAG TACCTCACAG CTCAGCCAAA CAGAAGTCGG AGGGAAGAAG
    CTGAAGAGCA CCATCCAGAG AAGTACGGAA ACAGGCCTGG CCGTGGAAAT GAGAAACTGG
    ATGAGCCGCC AAGCAAGCAG GGAGTCCACA GATGGGAGTA TGAACAGCTA CAGCTCAGAG
    GGGAATCTGA TATTCCCTGG GGTCCGACTG GGTTCCGACA GCCAGTTCAG TGACTTTTTG
    GATGGTCTTG GTCCTGCCCA GCTGGTTGGT CGCCAGACTT TGGCCACGCC CTCTATGGGA
    GATATTCAGG TTGGAATGGC TGACAAAAAA GGCCAGTTGG AGGTGGAGGT GATACGAGCC
    CGTGGTCTTG TTGTAAAACC AGGCTCAAAG ACACTGCCAG CACCCTATGT TAAGGTGTAT
    CTCTTAGAAA ATGGAGTGTG CATAGCCAAA AAGAAAACAA AAGTAGCAAG AAAAACGCTG
    GAGCCTCTAT ACCAGCAAGT GTTAGCGTTT GAGGAATCAC CACAAGGGAA GGTCCTACAG
    ATCATTGTTT GGGGTGACTA TGGACGCATG GACCACAAGT CCTTCATGGG GGTGGCTCAG
    ATACTTTTAG ATGAACTGGA CCTATCCAGC ATGGTGATTG GATGGTATAA ACTTTTCCCC
    CCTTCATCTT TAGTAGACCC AACGTTGGCT CCACTCACTA GGAGAGCTTC CCAATCATCT
    CTTGAAAGCT CCACGGGACC TTCTTATGCT CGTTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031759416.1
    CDS422..5620
    Translation

    Target ORF information:

    RefSeq Version XM_031903556.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 2 (rims2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031903556.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTCTGCTC CACTAGGGCC CAGGGGCGGC CCTCTGCCTC CTGCACAGCC ACAGGCTCCA 
    ATGCCAGAGA TGCCGGACCT CAGCCACCTG ACAGAGGATG AGAGGAAAAT CATAATGGCT
    GTGATGGAGA GGCAGAAGAA AGAAGAGGAG AAGGAGCAGT CAGTGTTAAA GGTCAAAGAA
    GAACAAAAAG CCCAGCCGGC ACAGTGGTTT CCCTTTAGTG GGATCACTGA GCTGGTTAAT
    AACGTTCTTC AGCCACAGCC AAAAGAGCAA AATGAAAAGG AGCCGGAGAT AAAATTGCAC
    CAGCAGTTTG AAATGTACAA GGAGCAAGTG AAGAAGATGG GCGAGGAAGC ACAGCAGCAG
    CAGGAGCAGA AGGGCGAAGC TCCAACCTGT GGAATCTGCC ACAAAACAAA GTTTGCTGAT
    GGCTGTGGCC ACGACTGTTC GTATTGCCAA ACTAAATTCT GCGCGCGGTG TGGAGGGCGA
    GTGTCTCTGC GCTCAAATAA GGAGGACAAA GTGGTAATGT GGGTATGTAA TTTGTGCCGA
    AAACAACAAG AAATCCTCAC CAAATCAGGG GCCTGGTTTT ACAGCAGTGG GTCAAGCACA
    CCCCTAAAGC CCGGCGAAGA GAGCCAGGCA GGGTACCAAA CGGAAGAAGC ACCTCAGGAG
    AAAAAAGCTA AGCTACAAGA GCAGTCCCAA TACCAGGGAC CCTCAGGGGA CTTATTAACA
    CCAGCCTCGG ACAAAAACCG ACCGCAAGGG ATCGTCCGGC AAGATTCCAT CAAAAATGGT
    TCCGAACTGA AGCACCCGAT TCCTGGCGAC CTCGCATCTG ACAGGAAAAG AAGCCCTTCA
    ATACCTAGAG ACCAGAATCG GAGATATGAT ACAAGGGACG ATATAGGTGA ACATTCACAG
    CATGCTACAT CAGACAGCGC CATGCCTAGA TCACCATCAG ACTACTCGGA CAGGCGATTG
    CAACGAGGGT CTCAGCTCTA TGATGAGCCT GAGCACGGTG ACTACAGAAG AAGTAGGCGG
    CGCTCCAATG AGTATTCGGT AGATGAGGAG GATATCCAAG ACAGGGAGGA ATATGAAAGG
    CAGAGAAGAG AGGAGGAGTA CCAGGCAAGG TACCGGAGTG ACCCCAACTT GGCCAGGTAC
    CCTGTTAAGC CACAACCGTA TGAAGAGCAA ATGCGCATCC ATGCAGAGGT TTCAAGGGCT
    CGACAACGAA GGCACAGCGA TGTGTCTTTA GCTCAGACAG AATTTGAGGA GTCTCGGATG
    TCAACGCTGA GGATGGAGAG ACCTTCAAGG CAAAGATCTG CGTCAGAGCG TAGAGCAGCG
    ATGGAAAGCC AGCGTTCATA TTCAATAGAA AGAACTAGAG ATGTGCAAGG GCCAGGTCCT
    GGTCGGCAAA GGACCTCAAA TCATAGTCCC CCAACACCAA GAAGGAGTCC CATTCCCAAT
    GATAGGCCAG ATATGAGGCG CACAGATTCT TTACGGAAAC AGCACCACTT GGACCCAAGT
    TCTGCCGTAA GGAAAACCAA AAGGGAAAAA ATGGAAACTA TGTTAAGGAA TGATTCTCTT
    AGCTCAGATC AGTCTGAATC TGTCCGGCCC CCACCACCAA AGCCTCATAA AACAAAAAAG
    GGAGGTAAAA TGCGTCAGGT TTCCTTGAGC AGTTCAGAAG AAGAGTTGGC GTCTACGCCG
    GAGTACACGA GCTGCGATGA TGTGGAGATT GAAAGTGAAA GTGTTAGTGA GAAAGGGGAC
    AGTCAAAGGG GAAAAAGAAA AACTAGTGAG CAGGCAGTTT TGTCGGATTC TAACATCTTG
    TCTGAGAGAC AAAAGAAAAT GGTGTGCTTT GGAGGCCACT CTTTGGAAGA GGATTTGGAA
    TGGTCTGAGC CTCAGATTAA AGACTCTGGG GTAGACACGT GTAGTAGCAC CACCCTTAAC
    GAAGAGCATA GCCATAGCGA GAAGCACCCT GTCACGTGGC AACCTTCCAA AGATGGAGAC
    CGCTTAATTG GTCGCATATT ATTAAACAAG CGGCTCAAAG ATGGGAGCGT CCCGCGAGAT
    TCTGGAGCCA TGCTGGGGCT GAAGGTCGTT GGAGGGAAAA TGACAGAATC AGGTCGACTT
    TGTGCGTTTA TCACCAAAGT GAAGAAGGGA AGCCTGGCAG ATACAGTAGG CCATCTTCGA
    CCAGGTGATG AGGTTTTAGA ATGGAGTGGG CGGATTTTGC AAGGAGCGAC GTTCGAAGAA
    GTTTACAATA TTATACTGGA GTCCAAACCC GAGGCACAAG TAGAATTATT GGTCTCCAGA
    CCCATAGGAG ATATACCCCG GATTCCTGAC AGCACACACG CACAACTGGA ATCCAGTTCT
    AGCTCATTTG AATCCCAGAA AATGGATCGC CCTTCTATTT CAGTTACTTC CCCCATGAGT
    CCTGGAATGT TACGGGATGT CCCCCAGTTT CTTTCTGGAC AACTTTCAAG CCAAAGCCTT
    AATAGAAGAA CAGCGCCTTT TGTTCCTAGG GTTCAGATTA AACTCTGGTA TGACAAGGTT
    GGCCACCAGT TAATTGTCAC GATATTAGGA GCAAAGGATC TGCCATCGCG GGAAGATGGG
    AGGCCGAGAA ATCCTTACGT AAAAATTTAC TTTCTTCCAG ATCGAAGTGA TAAGAACAAA
    AGGAGAACAA AGACTGTGAA GAAAACCGTA GAGCCCAAAT GGAACCAAAC ATTCATCTAT
    TCTCCGGTTC ATCGGAGGGA ATTTCGGGAA CGAATGTTGG AAATAACACT TTGGGATCAA
    GCTCGGGTTC GAGAAGAAGA GAGTGAATTT TTAGGAGAGA TTCTAATTGA ATTAGAAACT
    GCACTATTAG ATGATGAGCC ACATTGGTAC AAGCTACAGA CGCATGATGT GTCTTCATTG
    CCACTTCCAC ACCCATCCCC GTTCATGCAA AGAAGGCAGC TGCACGGCGA GAGCCCAACC
    CGGAGGTTGC AAAATAAAGG CTCATATTCA TATAATTCAG GATCCAAAAG GATAAGCGAC
    AGCGAAGTGT CTGACTACGA CTGTGATGAT GGCGTTGGTG TTGTTTCAGA CTTTCGGCAT
    AATGGGCGTG ACCTGCAGAG TTCAACGTTA TCTGTTCCGG AACAAGTAAT GTCGTCCAAT
    CACTGTTCCC GGACTGGGCC CCCGCATGTA GACAGCATAG GAAGAACGAG GTCATGGTCT
    CCCAGTGTCC CTCCTCCGCA AAGTAGGAAT TTGGAACAGG GACCGAGAGG GACAAGATCT
    GGCCCTGCAC ATTACAATAC CATGAGCAGA GTGGAAAGGC ATCGAGTATT AGATAACCAC
    TACTCCCCAG ACAGAGACAG GAATTGTGAA GCGGCAGATA GACAGCCATA TCAAAGATCC
    AGGTCCACTG AACAACGGCC TGTGCTGGAG CGGACCAGCT CCCGCTCCCG ATCTACTGAG
    CGTCCTGACT CAAACCTCAT GAGGTCGATG CCTTCATTAA TGACGGGAAG ATCTGCCCCT
    CCTTCACCCG CCTTATCGAG GGCACACCCT CCTCGTACCG GCTCTGTTCA GACAAGTCCT
    TCAGGCACCC CGTTAGCAGG GCGGAGGGGG CGCCAGCTCC CGCAACTTCC CCCAAAAGGC
    ACGTTAGAAA GAAAAGATAT GGACTCAACG CGGAGGCGAA ACTCAGAGGA AAAGGCTGCA
    GAACAAGAGA ATGGGAACCC AGGTGCTATG GACGTAGAAG AAAGGACGCG ACAGCTGAAA
    GTGAAGATCA ACAAATACAA GCAGGTGGGA GGATCAGATT CTCGGCTAGA GAAGGATTAT
    CATCCAAAGT ATCGGACGGG GCGGGATCCG CAGAGGGGCT CAGACAATAT GTCCAACAAG
    TCATCAGACA GCGATGTTAG TGATGTGTCT GCCGTATCAC GGACCAGCAG CGCCTCTCGT
    TTTAGTAGTA CGAGTTATAT GTCTGTGCAG TCGGAAAGGC CTCGAGGGAA CATGAAAATA
    GGTAGAAGGA CAAGGGAAGT AGAGGAGAGA ATAGAGAGGG CTGAGGGAGG GCAGCATCAC
    AAAGAAATAG GTTTTCTGGA GGAAGAGGAG GACGTTAGGC AGGAAGTAGA TAGTGAGAAG
    GAAACAGAAG TGCAGCATAT AATTGACAAC TGCATGAAGG GGCCAAGCAA AGAAAGTGTA
    GAGGAAGAGA GTCAACAGAA TGAGAAAGAT GAGGAAAGCC ATGATGTTGA GGAAGCCCAT
    AATGAGAATC TGCCAAGGAG GTCCTCAGAA AATGATATAA GTGCATTGGC ATCCAAAATG
    CAAAGCAGGC AAATGGGCGC CTCAGGGAAG AACATGACCA AAAGTACCAG CATCGGAGGG
    GATATGTACA CCCTGGAGAA GAACGACGGA AGCCAGTCTG ACACAGCCGT GGGCACTGTG
    GGTGCGGGTG GAAAGAAGAG ACGTTCAAGC ATAGGCGCCA AAATGGTGGC CATTGTGGGC
    TTGTCACGAA AAAGCCGAAG TACCTCACAG CTCAGCCAAA CAGAAGTCGG AGGGAAGAAG
    CTGAAGAGCA CCATCCAGAG AAGTACGGAA ACAGGCCTGG CCGTGGAAAT GAGAAACTGG
    ATGAGCCGCC AAGCAAGCAG GGAGTCCACA GATGGGAGTA TGAACAGCTA CAGCTCAGAG
    GGGAATCTGA TATTCCCTGG GGTCCGACTG GGTTCCGACA GCCAGTTCAG TGACTTTTTG
    GATGGTCTTG GTCCTGCCCA GCTGGTTGGT CGCCAGACTT TGGCCACGCC CTCTATGGGA
    GATATTCAGG TTGGAATGGC TGACAAAAAA GGCCAGTTGG AGGTGGAGGT GATACGAGCC
    CGTGGTCTTG TTGTAAAACC AGGCTCAAAG ACACTGCCAG CACCCTATGT TAAGGTGTAT
    CTCTTAGAAA ATGGAGTGTG CATAGCCAAA AAGAAAACAA AAGTAGCAAG AAAAACGCTG
    GAGCCTCTAT ACCAGCAAGT GTTAGCGTTT GAGGAATCAC CACAAGGGAA GGTCCTACAG
    ATCATTGTTT GGGGTGACTA TGGACGCATG GACCACAAGT CCTTCATGGG GGTGGCTCAG
    ATACTTTTAG ATGAACTGGA CCTATCCAGC ATGGTGATTG GATGGTATAA ACTTTTCCCC
    CCTTCATCTT TAGTAGACCC AACGTTGGCT CCACTCACTA GGAGAGCTTC CCAATCATCT
    CTTGAAAGCT CCACGGGACC TTCTTATGCT CGTTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa59898
    Clone ID Related Accession (Same CDS sequence) XM_031903557.1
    Accession Version XM_031903557.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5169bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 2 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTCTGCTC CACTAGGGCC CAGGGGCGGC CCTCTGCCTC CTGCACAGCC ACAGGCTCCA 
    ATGCCAGAGA TGCCGGACCT CAGCCACCTG ACAGAGGATG AGAGGAAAAT CATAATGGCT
    GTGATGGAGA GGCAGAAGAA AGAAGAGGAG AAGGAGCAGT CAGTGTTAAA AAAATTGCAC
    CAGCAGTTTG AAATGTACAA GGAGCAAGTG AAGAAGATGG GCGAGGAAGC ACAGCAGCAG
    CAGGAGCAGA AGGGCGAAGC TCCAACCTGT GGAATCTGCC ACAAAACAAA GTTTGCTGAT
    GGCTGTGGCC ACGACTGTTC GTATTGCCAA ACTAAATTCT GCGCGCGGTG TGGAGGGCGA
    GTGTCTCTGC GCTCAAATAA GGAGGACAAA GTGGTAATGT GGGTATGTAA TTTGTGCCGA
    AAACAACAAG AAATCCTCAC CAAATCAGGG GCCTGGTTTT ACAGCAGTGG GTCAAGCACA
    CCCCTAAAGC CCGGCGAAGA GAGCCAGGCA GGGTACCAAA CGGAAGAAGC ACCTCAGGAG
    AAAAAAGCTA AGCTACAAGA GCAGTCCCAA TACCAGGGAC CCTCAGGGGA CTTATTAACA
    CCAGCCTCGG ACAAAAACCG ACCGCAAGGG ATCGTCCGGC AAGATTCCAT CAAAAATGGT
    TCCGAACTGA AGCACCCGAT TCCTGGCGAC CTCGCATCTG ACAGGAAAAG AAGCCCTTCA
    ATACCTAGAG ACCAGAATCG GAGATATGAT ACAAGGGACG ATATAGGTGA ACATTCACAG
    CATGCTACAT CAGACAGCGC CATGCCTAGA TCACCATCAG ACTACTCGGA CAGGCGATTG
    CAACGAGGGT CTCAGCTCTA TGATGAGCCT GAGCACGGTG ACTACAGAAG AAGTAGGCGG
    CGCTCCAATG AGTATTCGGT AGATGAGGAG GATATCCAAG ACAGGGAGGA ATATGAAAGG
    CAGAGAAGAG AGGAGGAGTA CCAGGCAAGG TACCGGAGTG ACCCCAACTT GGCCAGGTAC
    CCTGTTAAGC CACAACCGTA TGAAGAGCAA ATGCGCATCC ATGCAGAGGT TTCAAGGGCT
    CGACAACGAA GGCACAGCGA TGTGTCTTTA GCTCAGACAG AATTTGAGGA GTCTCGGATG
    TCAACGCTGA GGATGGAGAG ACCTTCAAGG CAAAGATCTG CGTCAGAGCG TAGAGCAGCG
    ATGGAAAGCC AGCGTTCATA TTCAATAGAA AGAACTAGAG ATGTGCAAGG GCCAGGTCCT
    GGTCGGCAAA GGACCTCAAA TCATAGTCCC CCAACACCAA GAAGGAGTCC CATTCCCAAT
    GATAGGCCAG ATATGAGGCG CACAGATTCT TTACGGAAAC AGCACCACTT GGACCCAAGT
    TCTGCCGTAA GGAAAACCAA AAGGGAAAAA ATGGAAACTA TGTTAAGGAA TGATTCTCTT
    AGCTCAGATC AGTCTGAATC TGTCCGGCCC CCACCACCAA AGCCTCATAA AACAAAAAAG
    GGAGGTAAAA TGCGTCAGGT TTCCTTGAGC AGTTCAGAAG AAGAGTTGGC GTCTACGCCG
    GAGTACACGA GCTGCGATGA TGTGGAGATT GAAAGTGAAA GTGTTAGTGA GAAAGGGGAC
    AGTCAAAGGG GAAAAAGAAA AACTAGTGAG CAGGCAGTTT TGTCGGATTC TAACATCTTG
    TCTGAGAGAC AAAAGAAAAT GGTGTGCTTT GGAGGCCACT CTTTGGAAGA GGATTTGGAA
    TGGTCTGAGC CTCAGATTAA AGACTCTGGG GTAGACACGT GTAGTAGCAC CACCCTTAAC
    GAAGAGCATA GCCATAGCGA GAAGCACCCT GTCACGTGGC AACCTTCCAA AGATGGAGAC
    CGCTTAATTG GTCGCATATT ATTAAACAAG CGGCTCAAAG ATGGGAGCGT CCCGCGAGAT
    TCTGGAGCCA TGCTGGGGCT GAAGGTCGTT GGAGGGAAAA TGACAGAATC AGGTCGACTT
    TGTGCGTTTA TCACCAAAGT GAAGAAGGGA AGCCTGGCAG ATACAGTAGG CCATCTTCGA
    CCAGGTGATG AGGTTTTAGA ATGGAGTGGG CGGATTTTGC AAGGAGCGAC GTTCGAAGAA
    GTTTACAATA TTATACTGGA GTCCAAACCC GAGGCACAAG TAGAATTATT GGTCTCCAGA
    CCCATAGGAG ATATACCCCG GATTCCTGAC AGCACACACG CACAACTGGA ATCCAGTTCT
    AGCTCATTTG AATCCCAGAA AATGGATCGC CCTTCTATTT CAGTTACTTC CCCCATGAGT
    CCTGGAATGT TACGGGATGT CCCCCAGTTT CTTTCTGGAC AACTTTCAAG CCAAAGCCTT
    AATAGAAGAA CAGCGCCTTT TGTTCCTAGG GTTCAGATTA AACTCTGGTA TGACAAGGTT
    GGCCACCAGT TAATTGTCAC GATATTAGGA GCAAAGGATC TGCCATCGCG GGAAGATGGG
    AGGCCGAGAA ATCCTTACGT AAAAATTTAC TTTCTTCCAG ATCGAAGTGA TAAGAACAAA
    AGGAGAACAA AGACTGTGAA GAAAACCGTA GAGCCCAAAT GGAACCAAAC ATTCATCTAT
    TCTCCGGTTC ATCGGAGGGA ATTTCGGGAA CGAATGTTGG AAATAACACT TTGGGATCAA
    GCTCGGGTTC GAGAAGAAGA GAGTGAATTT TTAGGAGAGA TTCTAATTGA ATTAGAAACT
    GCACTATTAG ATGATGAGCC ACATTGGTAC AAGCTACAGA CGCATGATGT GTCTTCATTG
    CCACTTCCAC ACCCATCCCC GTTCATGCAA AGAAGGCAGC TGCACGGCGA GAGCCCAACC
    CGGAGGTTGC AAAATAAAGG CTCATATTCA TATAATTCAG GATCCAAAAG GATAAGCGAC
    AGCGAAGTGT CTGACTACGA CTGTGATGAT GGCGTTGGTG TTGTTTCAGA CTTTCGGCAT
    AATGGGCGTG ACCTGCAGAG TTCAACGTTA TCTGTTCCGG AACAAGTAAT GTCGTCCAAT
    CACTGTTCCC GGACTGGGCC CCCGCATGTA GACAGCATAG GAAGAACGAG GTCATGGTCT
    CCCAGTGTCC CTCCTCCGCA AAGTAGGAAT TTGGAACAGG GACCGAGAGG GACAAGATCT
    GGCCCTGCAC ATTACAATAC CATGAGCAGA GTGGAAAGGC ATCGAGTATT AGATAACCAC
    TACTCCCCAG ACAGAGACAG GAATTGTGAA GCGGCAGATA GACAGCCATA TCAAAGATCC
    AGGTCCACTG AACAACGGCC TGTGCTGGAG CGGACCAGCT CCCGCTCCCG ATCTACTGAG
    CGTCCTGACT CAAACCTCAT GAGGTCGATG CCTTCATTAA TGACGGGAAG ATCTGCCCCT
    CCTTCACCCG CCTTATCGAG GGCACACCCT CCTCGTACCG GCTCTGTTCA GACAAGTCCT
    TCAGGCACCC CGTTAGCAGG GCGGAGGGGG CGCCAGCTCC CGCAACTTCC CCCAAAAGGC
    ACGTTAGAAA GAAAAGTTAC TTGGGAAGAC CAGCAAAGAA AGGTGAATGG TTCAATAACT
    GATGACAAAC ATTTGCCAAG AAAGAAACAG CATTCTGAGC CAGAAGATAT GGACTCAACG
    CGGAGGCGAA ACTCAGAGGA AAAGGCTGCA GAACAAGAGA ATGGGAACCC AGGTGCTATG
    GACGTAGAAG AAAGGACGCG ACAGCTGAAA GTGAAGATCA ACAAATACAA GCAGGTGGGA
    GGATCAGATT CTCGGCTAGA GAAGGATTAT CATCCAAAGT ATCGGACGGG GCGGGATCCG
    CAGAGGGGCT CAGACAATAT GTCCAACAAG TCATCAGACA GCGATGTTAG TGATGTGTCT
    GCCGTATCAC GGACCAGCAG CGCCTCTCGT TTTAGTAGTA CGAGTTATAT GTCTGTGCAG
    TCGGAAAGGC CTCGAGGGAA CATGAAAATA GGTAGAAGGA CAAGGGAAGT AGAGGAGAGA
    ATAGAGAGGG CTGAGGGAGG GCAGCATCAC AAAGAAATAG GTTTTCTGGA GGAAGAGGAG
    GACGTTAGGC AGGAAGTAGA TAGTGAGAAG GAAACAGAAG TGCAGCATAT AATTGACAAC
    TGCATGAAGG GGCCAAGCAA AGAAAGTGTA GAGGAAGAGA GTCAACAGAA TGAGAAAGAT
    GAGGAAAGCC ATGATGTTGA GGAAGCCCAT AATGAGAATC TGCCAAGGAG GTCCTCAGAA
    AATGATATAA GTGCATTGGC ATCCAAAATG CAAAGCAGGC AAATGGGCGC CTCAGGGAAG
    AACATGACCA AAAGTACCAG CATCGGAGGG GATATGTACA CCCTGGAGAA GAACGACGGA
    AGCCAGTCTG ACACAGCCGT GGGCACTGTG GGTGCGGGTG GAAAGAAGAG ACGTTCAAGC
    ATAGGCGCCA AAATGGTGGC CATTGTGGGC TTGTCACGAA AAAGCCGAAG TACCTCACAG
    CTCAGCCAAA CAGAAGTCGG AGGGAAGAAG CTGAAGAGCA CCATCCAGAG AAGTACGGAA
    ACAGGCCTGG CCGTGGAAAT GAGAAACTGG ATGAGCCGCC AAGCAAGCAG GGAGTCCACA
    GATGGGAGTA TGAACAGCTA CAGCTCAGAG GGGAATCTGA TATTCCCTGG GGTCCGACTG
    GGTTCCGACA GCCAGTTCAG TGACTTTTTG GATGGTCTTG GTCCTGCCCA GCTGGTTGGT
    CGCCAGACTT TGGCCACGCC CTCTATGGGA GATATTCAGG TTGGAATGGC TGACAAAAAA
    GGCCAGTTGG AGGTGGAGGT GATACGAGCC CGTGGTCTTG TTGTAAAACC AGGCTCAAAG
    ACACTGCCAG CACCCTATGT TAAGGTGTAT CTCTTAGAAA ATGGAGTGTG CATAGCCAAA
    AAGAAAACAA AAGTAGCAAG AAAAACGCTG GAGCCTCTAT ACCAGCAAGT GTTAGCGTTT
    GAGGAATCAC CACAAGGGAA GGTCCTACAG ATCATTGTTT GGGGTGACTA TGGACGCATG
    GACCACAAGT CCTTCATGGG GGTGGCTCAG ATACTTTTAG ATGAACTGGA CCTATCCAGC
    ATGGTGATTG GATGGTATAA ACTTTTCCCC CCTTCATCTT TAGTAGACCC AACGTTGGCT
    CCACTCACTA GGAGAGCTTC CCAATCATCT CTTGAAAGCT CCACGGGACC TTCTTATGCT
    CGTTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031759417.1
    CDS422..5590
    Translation

    Target ORF information:

    RefSeq Version XM_031903557.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 2 (rims2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031903557.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTCTGCTC CACTAGGGCC CAGGGGCGGC CCTCTGCCTC CTGCACAGCC ACAGGCTCCA 
    ATGCCAGAGA TGCCGGACCT CAGCCACCTG ACAGAGGATG AGAGGAAAAT CATAATGGCT
    GTGATGGAGA GGCAGAAGAA AGAAGAGGAG AAGGAGCAGT CAGTGTTAAA AAAATTGCAC
    CAGCAGTTTG AAATGTACAA GGAGCAAGTG AAGAAGATGG GCGAGGAAGC ACAGCAGCAG
    CAGGAGCAGA AGGGCGAAGC TCCAACCTGT GGAATCTGCC ACAAAACAAA GTTTGCTGAT
    GGCTGTGGCC ACGACTGTTC GTATTGCCAA ACTAAATTCT GCGCGCGGTG TGGAGGGCGA
    GTGTCTCTGC GCTCAAATAA GGAGGACAAA GTGGTAATGT GGGTATGTAA TTTGTGCCGA
    AAACAACAAG AAATCCTCAC CAAATCAGGG GCCTGGTTTT ACAGCAGTGG GTCAAGCACA
    CCCCTAAAGC CCGGCGAAGA GAGCCAGGCA GGGTACCAAA CGGAAGAAGC ACCTCAGGAG
    AAAAAAGCTA AGCTACAAGA GCAGTCCCAA TACCAGGGAC CCTCAGGGGA CTTATTAACA
    CCAGCCTCGG ACAAAAACCG ACCGCAAGGG ATCGTCCGGC AAGATTCCAT CAAAAATGGT
    TCCGAACTGA AGCACCCGAT TCCTGGCGAC CTCGCATCTG ACAGGAAAAG AAGCCCTTCA
    ATACCTAGAG ACCAGAATCG GAGATATGAT ACAAGGGACG ATATAGGTGA ACATTCACAG
    CATGCTACAT CAGACAGCGC CATGCCTAGA TCACCATCAG ACTACTCGGA CAGGCGATTG
    CAACGAGGGT CTCAGCTCTA TGATGAGCCT GAGCACGGTG ACTACAGAAG AAGTAGGCGG
    CGCTCCAATG AGTATTCGGT AGATGAGGAG GATATCCAAG ACAGGGAGGA ATATGAAAGG
    CAGAGAAGAG AGGAGGAGTA CCAGGCAAGG TACCGGAGTG ACCCCAACTT GGCCAGGTAC
    CCTGTTAAGC CACAACCGTA TGAAGAGCAA ATGCGCATCC ATGCAGAGGT TTCAAGGGCT
    CGACAACGAA GGCACAGCGA TGTGTCTTTA GCTCAGACAG AATTTGAGGA GTCTCGGATG
    TCAACGCTGA GGATGGAGAG ACCTTCAAGG CAAAGATCTG CGTCAGAGCG TAGAGCAGCG
    ATGGAAAGCC AGCGTTCATA TTCAATAGAA AGAACTAGAG ATGTGCAAGG GCCAGGTCCT
    GGTCGGCAAA GGACCTCAAA TCATAGTCCC CCAACACCAA GAAGGAGTCC CATTCCCAAT
    GATAGGCCAG ATATGAGGCG CACAGATTCT TTACGGAAAC AGCACCACTT GGACCCAAGT
    TCTGCCGTAA GGAAAACCAA AAGGGAAAAA ATGGAAACTA TGTTAAGGAA TGATTCTCTT
    AGCTCAGATC AGTCTGAATC TGTCCGGCCC CCACCACCAA AGCCTCATAA AACAAAAAAG
    GGAGGTAAAA TGCGTCAGGT TTCCTTGAGC AGTTCAGAAG AAGAGTTGGC GTCTACGCCG
    GAGTACACGA GCTGCGATGA TGTGGAGATT GAAAGTGAAA GTGTTAGTGA GAAAGGGGAC
    AGTCAAAGGG GAAAAAGAAA AACTAGTGAG CAGGCAGTTT TGTCGGATTC TAACATCTTG
    TCTGAGAGAC AAAAGAAAAT GGTGTGCTTT GGAGGCCACT CTTTGGAAGA GGATTTGGAA
    TGGTCTGAGC CTCAGATTAA AGACTCTGGG GTAGACACGT GTAGTAGCAC CACCCTTAAC
    GAAGAGCATA GCCATAGCGA GAAGCACCCT GTCACGTGGC AACCTTCCAA AGATGGAGAC
    CGCTTAATTG GTCGCATATT ATTAAACAAG CGGCTCAAAG ATGGGAGCGT CCCGCGAGAT
    TCTGGAGCCA TGCTGGGGCT GAAGGTCGTT GGAGGGAAAA TGACAGAATC AGGTCGACTT
    TGTGCGTTTA TCACCAAAGT GAAGAAGGGA AGCCTGGCAG ATACAGTAGG CCATCTTCGA
    CCAGGTGATG AGGTTTTAGA ATGGAGTGGG CGGATTTTGC AAGGAGCGAC GTTCGAAGAA
    GTTTACAATA TTATACTGGA GTCCAAACCC GAGGCACAAG TAGAATTATT GGTCTCCAGA
    CCCATAGGAG ATATACCCCG GATTCCTGAC AGCACACACG CACAACTGGA ATCCAGTTCT
    AGCTCATTTG AATCCCAGAA AATGGATCGC CCTTCTATTT CAGTTACTTC CCCCATGAGT
    CCTGGAATGT TACGGGATGT CCCCCAGTTT CTTTCTGGAC AACTTTCAAG CCAAAGCCTT
    AATAGAAGAA CAGCGCCTTT TGTTCCTAGG GTTCAGATTA AACTCTGGTA TGACAAGGTT
    GGCCACCAGT TAATTGTCAC GATATTAGGA GCAAAGGATC TGCCATCGCG GGAAGATGGG
    AGGCCGAGAA ATCCTTACGT AAAAATTTAC TTTCTTCCAG ATCGAAGTGA TAAGAACAAA
    AGGAGAACAA AGACTGTGAA GAAAACCGTA GAGCCCAAAT GGAACCAAAC ATTCATCTAT
    TCTCCGGTTC ATCGGAGGGA ATTTCGGGAA CGAATGTTGG AAATAACACT TTGGGATCAA
    GCTCGGGTTC GAGAAGAAGA GAGTGAATTT TTAGGAGAGA TTCTAATTGA ATTAGAAACT
    GCACTATTAG ATGATGAGCC ACATTGGTAC AAGCTACAGA CGCATGATGT GTCTTCATTG
    CCACTTCCAC ACCCATCCCC GTTCATGCAA AGAAGGCAGC TGCACGGCGA GAGCCCAACC
    CGGAGGTTGC AAAATAAAGG CTCATATTCA TATAATTCAG GATCCAAAAG GATAAGCGAC
    AGCGAAGTGT CTGACTACGA CTGTGATGAT GGCGTTGGTG TTGTTTCAGA CTTTCGGCAT
    AATGGGCGTG ACCTGCAGAG TTCAACGTTA TCTGTTCCGG AACAAGTAAT GTCGTCCAAT
    CACTGTTCCC GGACTGGGCC CCCGCATGTA GACAGCATAG GAAGAACGAG GTCATGGTCT
    CCCAGTGTCC CTCCTCCGCA AAGTAGGAAT TTGGAACAGG GACCGAGAGG GACAAGATCT
    GGCCCTGCAC ATTACAATAC CATGAGCAGA GTGGAAAGGC ATCGAGTATT AGATAACCAC
    TACTCCCCAG ACAGAGACAG GAATTGTGAA GCGGCAGATA GACAGCCATA TCAAAGATCC
    AGGTCCACTG AACAACGGCC TGTGCTGGAG CGGACCAGCT CCCGCTCCCG ATCTACTGAG
    CGTCCTGACT CAAACCTCAT GAGGTCGATG CCTTCATTAA TGACGGGAAG ATCTGCCCCT
    CCTTCACCCG CCTTATCGAG GGCACACCCT CCTCGTACCG GCTCTGTTCA GACAAGTCCT
    TCAGGCACCC CGTTAGCAGG GCGGAGGGGG CGCCAGCTCC CGCAACTTCC CCCAAAAGGC
    ACGTTAGAAA GAAAAGTTAC TTGGGAAGAC CAGCAAAGAA AGGTGAATGG TTCAATAACT
    GATGACAAAC ATTTGCCAAG AAAGAAACAG CATTCTGAGC CAGAAGATAT GGACTCAACG
    CGGAGGCGAA ACTCAGAGGA AAAGGCTGCA GAACAAGAGA ATGGGAACCC AGGTGCTATG
    GACGTAGAAG AAAGGACGCG ACAGCTGAAA GTGAAGATCA ACAAATACAA GCAGGTGGGA
    GGATCAGATT CTCGGCTAGA GAAGGATTAT CATCCAAAGT ATCGGACGGG GCGGGATCCG
    CAGAGGGGCT CAGACAATAT GTCCAACAAG TCATCAGACA GCGATGTTAG TGATGTGTCT
    GCCGTATCAC GGACCAGCAG CGCCTCTCGT TTTAGTAGTA CGAGTTATAT GTCTGTGCAG
    TCGGAAAGGC CTCGAGGGAA CATGAAAATA GGTAGAAGGA CAAGGGAAGT AGAGGAGAGA
    ATAGAGAGGG CTGAGGGAGG GCAGCATCAC AAAGAAATAG GTTTTCTGGA GGAAGAGGAG
    GACGTTAGGC AGGAAGTAGA TAGTGAGAAG GAAACAGAAG TGCAGCATAT AATTGACAAC
    TGCATGAAGG GGCCAAGCAA AGAAAGTGTA GAGGAAGAGA GTCAACAGAA TGAGAAAGAT
    GAGGAAAGCC ATGATGTTGA GGAAGCCCAT AATGAGAATC TGCCAAGGAG GTCCTCAGAA
    AATGATATAA GTGCATTGGC ATCCAAAATG CAAAGCAGGC AAATGGGCGC CTCAGGGAAG
    AACATGACCA AAAGTACCAG CATCGGAGGG GATATGTACA CCCTGGAGAA GAACGACGGA
    AGCCAGTCTG ACACAGCCGT GGGCACTGTG GGTGCGGGTG GAAAGAAGAG ACGTTCAAGC
    ATAGGCGCCA AAATGGTGGC CATTGTGGGC TTGTCACGAA AAAGCCGAAG TACCTCACAG
    CTCAGCCAAA CAGAAGTCGG AGGGAAGAAG CTGAAGAGCA CCATCCAGAG AAGTACGGAA
    ACAGGCCTGG CCGTGGAAAT GAGAAACTGG ATGAGCCGCC AAGCAAGCAG GGAGTCCACA
    GATGGGAGTA TGAACAGCTA CAGCTCAGAG GGGAATCTGA TATTCCCTGG GGTCCGACTG
    GGTTCCGACA GCCAGTTCAG TGACTTTTTG GATGGTCTTG GTCCTGCCCA GCTGGTTGGT
    CGCCAGACTT TGGCCACGCC CTCTATGGGA GATATTCAGG TTGGAATGGC TGACAAAAAA
    GGCCAGTTGG AGGTGGAGGT GATACGAGCC CGTGGTCTTG TTGTAAAACC AGGCTCAAAG
    ACACTGCCAG CACCCTATGT TAAGGTGTAT CTCTTAGAAA ATGGAGTGTG CATAGCCAAA
    AAGAAAACAA AAGTAGCAAG AAAAACGCTG GAGCCTCTAT ACCAGCAAGT GTTAGCGTTT
    GAGGAATCAC CACAAGGGAA GGTCCTACAG ATCATTGTTT GGGGTGACTA TGGACGCATG
    GACCACAAGT CCTTCATGGG GGTGGCTCAG ATACTTTTAG ATGAACTGGA CCTATCCAGC
    ATGGTGATTG GATGGTATAA ACTTTTCCCC CCTTCATCTT TAGTAGACCC AACGTTGGCT
    CCACTCACTA GGAGAGCTTC CCAATCATCT CTTGAAAGCT CCACGGGACC TTCTTATGCT
    CGTTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa59899
    Clone ID Related Accession (Same CDS sequence) XM_031903558.1
    Accession Version XM_031903558.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5163bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 2 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTCTGCTC CACTAGGGCC CAGGGGCGGC CCTCTGCCTC CTGCACAGCC ACAGGCTCCA 
    ATGCCAGAGA TGCCGGACCT CAGCCACCTG ACAGAGGATG AGAGGAAAAT CATAATGGCT
    GTGATGGAGA GGCAGAAGAA AGAAGAGGAG AAGGAGCAGT CAGTGTTAAA GGTCAAAGAA
    GAACAAAAAG CCCAGCCGGC ACAGTGGTTT CCCTTTAGTG GGATCACTGA GCTGGTTAAT
    AACGTTCTTC AGCCACAGCC AAAAGAGCAA AATGAAAAGG AGCCGGAGAT AAAATTGCAC
    CAGCAGTTTG AAATGTACAA GGAGCAAGTG AAGAAGATGG GCGAGGAAGC ACAGCAGCAG
    CAGGAGCAGA AGGGCGAAGC TCCAACCTGT GGAATCTGCC ACAAAACAAA GTTTGCTGAT
    GGCTGTGGCC ACGACTGTTC GTATTGCCAA ACTAAATTCT GCGCGCGGTG TGGAGGGCGA
    GTGTCTCTGC GCTCAAATAA GGAGGACAAA GTGGTAATGT GGGTATGTAA TTTGTGCCGA
    AAACAACAAG AAATCCTCAC CAAATCAGGG GCCTGGTTTT ACAGCAGTGG GTCAAGCACA
    CCCCTAAAGC CCGGCGAAGA GAGCCAGGCA GGGTACCAAA CGGAAGAAGC ACCTCAGGAG
    AAAAAAGCTA AGCTACAAGA GCAGTCCCAA TACCAGGGAC CCTCAGGGGA CTTATTAACA
    CCAGCCTCGG ACAAAAACCG ACCGCAAGGG ATCGTCCGGC AAGATTCCAT CAAAAATGGT
    TCCGAACTGA AGCACCCGAT TCCTGGCGAC CTCGCATCTG ACAGGAAAAG AAGCCCTTCA
    ATACCTAGAG ACCAGAATCG GAGATATGAT ACAAGGGACG ATATAGGTGA ACATTCACAG
    CATGCTACAT CAGACAGCGC CATGCCTAGA TCACCATCAG ACTACTCGGA CAGGCGATTG
    CAACGAGGGT CTCAGCTCTA TGATGAGCCT GAGCACGGTG ACTACAGAAG AAGTAGGCGG
    CGCTCCAATG AGTATTCGGT AGATGAGGAG GATATCCAAG ACAGGGAGGA ATATGAAAGG
    CAGAGAAGAG AGGAGGAGTA CCAGGCAAGG TACCGGAGTG ACCCCAACTT GGCCAGGTAC
    CCTGTTAAGC CACAACCGTA TGAAGAGCAA ATGCGCATCC ATGCAGAGGT TTCAAGGGCT
    CGACAACGAA GGCACAGCGA TGTGTCTTTA GCTCAGACAG AATTTGAGGA GTCTCGGATG
    TCAACGCTGA GGATGGAGAG ACCTTCAAGG CAAAGATCTG CGTCAGAGCG TAGAGCAGCG
    ATGGAAAGCC AGCGTTCATA TTCAATAGAA AGAACTAGAG ATGTGCAAGG GCCAGGTCCT
    GGTCGGCAAA GGACCTCAAA TCATAGTCCC CCAACACCAA GAAGGAGTCC CATTCCCAAT
    GATAGGCCAG ATATGAGGCG CACAGATTCT TTACGGAAAC AGCACCACTT GGACCCAAGT
    TCTGCCGTAA GGAAAACCAA AAGGGAAAAA ATGGAAACTA TGTTAAGGAA TGATTCTCTT
    AGCTCAGATC AGTCTGAATC TGTCCGGCCC CCACCACCAA AGCCTCATAA AACAAAAAAG
    GGAGGTAAAA TGCGTCAGGT TTCCTTGAGC AGTTCAGAAG AAGAGTTGGC GTCTACGCCG
    GAGTACACGA GCTGCGATGA TGTGGAGATT GAAAGTGAAA GTGTTAGTGA GAAAGGGGAC
    AGTCAAAGGG GAAAAAGAAA AACTAGTGAG CAGGCAGTTT TGTCGGATTC TAACATCTTG
    TCTGAGAGAC AAAAGAAAAT GGTGTGCTTT GGAGGCCACT CTTTGGAAGA GGATTTGGAA
    TGGTCTGAGC CTCAGATTAA AGACTCTGGG GTAGACACGT GTAGTAGCAC CACCCTTAAC
    GAAGAGCATA GCCATAGCGA GAAGCACCCT GTCACGTGGC AACCTTCCAA AGATGGAGAC
    CGCTTAATTG GTCGCATATT ATTAAACAAG CGGCTCAAAG ATGGGAGCGT CCCGCGAGAT
    TCTGGAGCCA TGCTGGGGCT GAAGGTCGTT GGAGGGAAAA TGACAGAATC AGGTCGACTT
    TGTGCGTTTA TCACCAAAGT GAAGAAGGGA AGCCTGGCAG ATACAGTAGG CCATCTTCGA
    CCAGGTGATG AGGTTTTAGA ATGGAGTGGG CGGATTTTGC AAGGAGCGAC GTTCGAAGAA
    GTTTACAATA TTATACTGGA GTCCAAACCC GAGGCACAAG TAGAATTATT GGTCTCCAGA
    CCCATAGGAG ATATACCCCG GATTCCTGAC AGCACACACG CACAACTGGA ATCCAGTTCT
    AGCTCATTTG AATCCCAGAA AATGGATCGC CCTTCTATTT CAGTTACTTC CCCCATGAGT
    CCTGGAATGT TACGGGATGT CCCCCAGTTT CTTTCTGGAC AACTTTCAAG CCAAAGCCTT
    AATAGAAGAA CAGCGCCTTT TGTTCCTAGG GTTCAGATTA AACTCTGGTA TGACAAGGTT
    GGCCACCAGT TAATTGTCAC GATATTAGGA GCAAAGGATC TGCCATCGCG GGAAGATGGG
    AGGCCGAGAA ATCCTTACGT AAAAATTTAC TTTCTTCCAG ATCGAAGTGA TAAGAACAAA
    AGGAGAACAA AGACTGTGAA GAAAACCGTA GAGCCCAAAT GGAACCAAAC ATTCATCTAT
    TCTCCGGTTC ATCGGAGGGA ATTTCGGGAA CGAATGTTGG AAATAACACT TTGGGATCAA
    GCTCGGGTTC GAGAAGAAGA GAGTGAATTT TTAGGAGAGA TTCTAATTGA ATTAGAAACT
    GCACTATTAG ATGATGAGCC ACATTGGTAC AAGCTACAGA CGCATGATGT GTCTTCATTG
    CCACTTCCAC ACCCATCCCC GTTCATGCAA AGAAGGCAGC TGCACGGCGA GAGCCCAACC
    CGGAGGTTGC AAAATAAAGG CTCATATTCA TATAATTCAG GATCCAAAAG GATAAGCGAC
    AGCGAAGTGT CTGACTACGA CTGTGATGAT GGCGTTGGTG TTGTTTCAGA CTTTCGGCAT
    AATGGGCGTG ACCTGCAGAG TTCAACGTTA TCTGTTCCGG AACAAGTAAT GTCGTCCAAT
    CACTGTTCCC GGACTGGGCC CCCGCATGTA GACAGCATAG GAAGAACGAG GTCATGGTCT
    CCCAGTGTCC CTCCTCCGCA AAGTAGGAAT TTGGAACAGG GACCGAGAGG GACAAGATCT
    GGCCCTGCAC ATTACAATAC CATGAGCAGA GTGGAAAGGC ATCGAGTATT AGATAACCAC
    TACTCCCCAG ACAGAGACAG GAATTGTGAA GCGGCAGATA GACAGCCATA TCAAAGATCC
    AGGTCCACTG AACAACGGCC TGTGCTGGAG CGGACCAGCT CCCGCTCCCG ATCTACTGAG
    CGTCCTGACT CAAACCTCAT GAGGTCGATG CCTTCATTAA TGACGGGAAG ATCTGCCCCT
    CCTTCACCCG CCTTATCGAG GGCACACCCT CCTCGTACCG GCTCTGTTCA GACAAGTCCT
    TCAGGCACCC CGTTAGCAGG GCGGAGGGGG CGCCAGCTCC CGCAACTTCC CCCAAAAGGC
    ACGTTAGAAA GAAAAGATAT GGACTCAACG CGGAGGCGAA ACTCAGGTGC TATGGACGTA
    GAAGAAAGGA CGCGACAGCT GAAAGTGAAG ATCAACAAAT ACAAGCAGGT GGGAGGATCA
    GATTCTCGGC TAGAGAAGGA TTATCATCCA AAGTATCGGA CGGGGCGGGA TCCGCAGAGG
    GGCTCAGACA ATATGTCCAA CAAGTCATCA GACAGCGATG TTAGTGATGT GTCTGCCGTA
    TCACGGACCA GCAGCGCCTC TCGTTTTAGT AGTACGAGTT ATATGTCTGT GCAGTCGGAA
    AGGCCTCGAG GGAACATGAA AATAGGTAGA AGGACAAGGG AAGTAGAGGA GAGAATAGAG
    AGGGCTGAGG GAGGGCAGCA TCACAAAGAA ATAGGTTTTC TGGAGGAAGA GGAGGACGTT
    AGGCAGGAAG TAGATAGTGA GAAGGAAACA GAAGTGCAGC ATATAATTGA CAACTGCATG
    AAGGGGCCAA GCAAAGAAAG TGTAGAGGAA GAGAGTCAAC AGAATGAGAA AGATGAGGAA
    AGCCATGATG TTGAGGAAGC CCATAATGAG AATCTGCCAA GGAGGTCCTC AGAAAATGAT
    ATAAGTGCAT TGGCATCCAA AATGCAAAGC AGGCAAATGG GCGCCTCAGG GAAGAACATG
    ACCAAAAGTA CCAGCATCGG AGGGGATATG TACACCCTGG AGAAGAACGA CGGAAGCCAG
    TCTGACACAG CCGTGGGCAC TGTGGGTGCG GGTGGAAAGA AGAGACGTTC AAGCATAGGC
    GCCAAAATGG TGGCCATTGT GGGCTTGTCA CGAAAAAGCC GAAGTACCTC ACAGCTCAGC
    CAAACAGAAG TCGGAGGGAA GAAGCTGAAG AGCACCATCC AGAGAAGTAC GGAAACAGGC
    CTGGCCGTGG AAATGAGAAA CTGGATGAGC CGCCAAGCAA GCAGGGAGTC CACAGATGGG
    AGTATGAACA GCTACAGCTC AGAGGGGAAT CTGATATTCC CTGGGGTCCG ACTGGGTTCC
    GACAGCCAGT TCAGTGACTT TTTGGATGGT CTTGGTCCTG CCCAGCTGGT TGGTCGCCAG
    ACTTTGGCCA CGCCCTCTAT GGGAGATATT CAGGTTGGAA TGGCTGACAA AAAAGGCCAG
    TTGGAGGTGG AGGTGATACG AGCCCGTGGT CTTGTTGTAA AACCAGGCTC AAAGACACTG
    CCAGCACCCT ATGTTAAGGT GTATCTCTTA GAAAATGGAG TGTGCATAGC CAAAAAGAAA
    ACAAAAGTAG CAAGAAAAAC GCTGGAGCCT CTATACCAGC AAGTGTTAGC GTTTGAGGAA
    TCACCACAAG GGAAGGTCCT ACAGATCATT GTTTGGGGTG ACTATGGACG CATGGACCAC
    AAGTCCTTCA TGGGGGTGGC TCAGATACTT TTAGATGAAC TGGACCTATC CAGCATGGTG
    ATTGGATGGT ATAAACTTTT CCCCCCTTCA TCTTTAGTAG ACCCAACGTT GGCTCCACTC
    ACTAGGAGAG CTTCCCAATC ATCTCTTGAA AGCTCCACGG GACCTTCTTA TGCTCGTTCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031759418.1
    CDS422..5584
    Translation

    Target ORF information:

    RefSeq Version XM_031903558.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 2 (rims2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031903558.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTCTGCTC CACTAGGGCC CAGGGGCGGC CCTCTGCCTC CTGCACAGCC ACAGGCTCCA 
    ATGCCAGAGA TGCCGGACCT CAGCCACCTG ACAGAGGATG AGAGGAAAAT CATAATGGCT
    GTGATGGAGA GGCAGAAGAA AGAAGAGGAG AAGGAGCAGT CAGTGTTAAA GGTCAAAGAA
    GAACAAAAAG CCCAGCCGGC ACAGTGGTTT CCCTTTAGTG GGATCACTGA GCTGGTTAAT
    AACGTTCTTC AGCCACAGCC AAAAGAGCAA AATGAAAAGG AGCCGGAGAT AAAATTGCAC
    CAGCAGTTTG AAATGTACAA GGAGCAAGTG AAGAAGATGG GCGAGGAAGC ACAGCAGCAG
    CAGGAGCAGA AGGGCGAAGC TCCAACCTGT GGAATCTGCC ACAAAACAAA GTTTGCTGAT
    GGCTGTGGCC ACGACTGTTC GTATTGCCAA ACTAAATTCT GCGCGCGGTG TGGAGGGCGA
    GTGTCTCTGC GCTCAAATAA GGAGGACAAA GTGGTAATGT GGGTATGTAA TTTGTGCCGA
    AAACAACAAG AAATCCTCAC CAAATCAGGG GCCTGGTTTT ACAGCAGTGG GTCAAGCACA
    CCCCTAAAGC CCGGCGAAGA GAGCCAGGCA GGGTACCAAA CGGAAGAAGC ACCTCAGGAG
    AAAAAAGCTA AGCTACAAGA GCAGTCCCAA TACCAGGGAC CCTCAGGGGA CTTATTAACA
    CCAGCCTCGG ACAAAAACCG ACCGCAAGGG ATCGTCCGGC AAGATTCCAT CAAAAATGGT
    TCCGAACTGA AGCACCCGAT TCCTGGCGAC CTCGCATCTG ACAGGAAAAG AAGCCCTTCA
    ATACCTAGAG ACCAGAATCG GAGATATGAT ACAAGGGACG ATATAGGTGA ACATTCACAG
    CATGCTACAT CAGACAGCGC CATGCCTAGA TCACCATCAG ACTACTCGGA CAGGCGATTG
    CAACGAGGGT CTCAGCTCTA TGATGAGCCT GAGCACGGTG ACTACAGAAG AAGTAGGCGG
    CGCTCCAATG AGTATTCGGT AGATGAGGAG GATATCCAAG ACAGGGAGGA ATATGAAAGG
    CAGAGAAGAG AGGAGGAGTA CCAGGCAAGG TACCGGAGTG ACCCCAACTT GGCCAGGTAC
    CCTGTTAAGC CACAACCGTA TGAAGAGCAA ATGCGCATCC ATGCAGAGGT TTCAAGGGCT
    CGACAACGAA GGCACAGCGA TGTGTCTTTA GCTCAGACAG AATTTGAGGA GTCTCGGATG
    TCAACGCTGA GGATGGAGAG ACCTTCAAGG CAAAGATCTG CGTCAGAGCG TAGAGCAGCG
    ATGGAAAGCC AGCGTTCATA TTCAATAGAA AGAACTAGAG ATGTGCAAGG GCCAGGTCCT
    GGTCGGCAAA GGACCTCAAA TCATAGTCCC CCAACACCAA GAAGGAGTCC CATTCCCAAT
    GATAGGCCAG ATATGAGGCG CACAGATTCT TTACGGAAAC AGCACCACTT GGACCCAAGT
    TCTGCCGTAA GGAAAACCAA AAGGGAAAAA ATGGAAACTA TGTTAAGGAA TGATTCTCTT
    AGCTCAGATC AGTCTGAATC TGTCCGGCCC CCACCACCAA AGCCTCATAA AACAAAAAAG
    GGAGGTAAAA TGCGTCAGGT TTCCTTGAGC AGTTCAGAAG AAGAGTTGGC GTCTACGCCG
    GAGTACACGA GCTGCGATGA TGTGGAGATT GAAAGTGAAA GTGTTAGTGA GAAAGGGGAC
    AGTCAAAGGG GAAAAAGAAA AACTAGTGAG CAGGCAGTTT TGTCGGATTC TAACATCTTG
    TCTGAGAGAC AAAAGAAAAT GGTGTGCTTT GGAGGCCACT CTTTGGAAGA GGATTTGGAA
    TGGTCTGAGC CTCAGATTAA AGACTCTGGG GTAGACACGT GTAGTAGCAC CACCCTTAAC
    GAAGAGCATA GCCATAGCGA GAAGCACCCT GTCACGTGGC AACCTTCCAA AGATGGAGAC
    CGCTTAATTG GTCGCATATT ATTAAACAAG CGGCTCAAAG ATGGGAGCGT CCCGCGAGAT
    TCTGGAGCCA TGCTGGGGCT GAAGGTCGTT GGAGGGAAAA TGACAGAATC AGGTCGACTT
    TGTGCGTTTA TCACCAAAGT GAAGAAGGGA AGCCTGGCAG ATACAGTAGG CCATCTTCGA
    CCAGGTGATG AGGTTTTAGA ATGGAGTGGG CGGATTTTGC AAGGAGCGAC GTTCGAAGAA
    GTTTACAATA TTATACTGGA GTCCAAACCC GAGGCACAAG TAGAATTATT GGTCTCCAGA
    CCCATAGGAG ATATACCCCG GATTCCTGAC AGCACACACG CACAACTGGA ATCCAGTTCT
    AGCTCATTTG AATCCCAGAA AATGGATCGC CCTTCTATTT CAGTTACTTC CCCCATGAGT
    CCTGGAATGT TACGGGATGT CCCCCAGTTT CTTTCTGGAC AACTTTCAAG CCAAAGCCTT
    AATAGAAGAA CAGCGCCTTT TGTTCCTAGG GTTCAGATTA AACTCTGGTA TGACAAGGTT
    GGCCACCAGT TAATTGTCAC GATATTAGGA GCAAAGGATC TGCCATCGCG GGAAGATGGG
    AGGCCGAGAA ATCCTTACGT AAAAATTTAC TTTCTTCCAG ATCGAAGTGA TAAGAACAAA
    AGGAGAACAA AGACTGTGAA GAAAACCGTA GAGCCCAAAT GGAACCAAAC ATTCATCTAT
    TCTCCGGTTC ATCGGAGGGA ATTTCGGGAA CGAATGTTGG AAATAACACT TTGGGATCAA
    GCTCGGGTTC GAGAAGAAGA GAGTGAATTT TTAGGAGAGA TTCTAATTGA ATTAGAAACT
    GCACTATTAG ATGATGAGCC ACATTGGTAC AAGCTACAGA CGCATGATGT GTCTTCATTG
    CCACTTCCAC ACCCATCCCC GTTCATGCAA AGAAGGCAGC TGCACGGCGA GAGCCCAACC
    CGGAGGTTGC AAAATAAAGG CTCATATTCA TATAATTCAG GATCCAAAAG GATAAGCGAC
    AGCGAAGTGT CTGACTACGA CTGTGATGAT GGCGTTGGTG TTGTTTCAGA CTTTCGGCAT
    AATGGGCGTG ACCTGCAGAG TTCAACGTTA TCTGTTCCGG AACAAGTAAT GTCGTCCAAT
    CACTGTTCCC GGACTGGGCC CCCGCATGTA GACAGCATAG GAAGAACGAG GTCATGGTCT
    CCCAGTGTCC CTCCTCCGCA AAGTAGGAAT TTGGAACAGG GACCGAGAGG GACAAGATCT
    GGCCCTGCAC ATTACAATAC CATGAGCAGA GTGGAAAGGC ATCGAGTATT AGATAACCAC
    TACTCCCCAG ACAGAGACAG GAATTGTGAA GCGGCAGATA GACAGCCATA TCAAAGATCC
    AGGTCCACTG AACAACGGCC TGTGCTGGAG CGGACCAGCT CCCGCTCCCG ATCTACTGAG
    CGTCCTGACT CAAACCTCAT GAGGTCGATG CCTTCATTAA TGACGGGAAG ATCTGCCCCT
    CCTTCACCCG CCTTATCGAG GGCACACCCT CCTCGTACCG GCTCTGTTCA GACAAGTCCT
    TCAGGCACCC CGTTAGCAGG GCGGAGGGGG CGCCAGCTCC CGCAACTTCC CCCAAAAGGC
    ACGTTAGAAA GAAAAGATAT GGACTCAACG CGGAGGCGAA ACTCAGGTGC TATGGACGTA
    GAAGAAAGGA CGCGACAGCT GAAAGTGAAG ATCAACAAAT ACAAGCAGGT GGGAGGATCA
    GATTCTCGGC TAGAGAAGGA TTATCATCCA AAGTATCGGA CGGGGCGGGA TCCGCAGAGG
    GGCTCAGACA ATATGTCCAA CAAGTCATCA GACAGCGATG TTAGTGATGT GTCTGCCGTA
    TCACGGACCA GCAGCGCCTC TCGTTTTAGT AGTACGAGTT ATATGTCTGT GCAGTCGGAA
    AGGCCTCGAG GGAACATGAA AATAGGTAGA AGGACAAGGG AAGTAGAGGA GAGAATAGAG
    AGGGCTGAGG GAGGGCAGCA TCACAAAGAA ATAGGTTTTC TGGAGGAAGA GGAGGACGTT
    AGGCAGGAAG TAGATAGTGA GAAGGAAACA GAAGTGCAGC ATATAATTGA CAACTGCATG
    AAGGGGCCAA GCAAAGAAAG TGTAGAGGAA GAGAGTCAAC AGAATGAGAA AGATGAGGAA
    AGCCATGATG TTGAGGAAGC CCATAATGAG AATCTGCCAA GGAGGTCCTC AGAAAATGAT
    ATAAGTGCAT TGGCATCCAA AATGCAAAGC AGGCAAATGG GCGCCTCAGG GAAGAACATG
    ACCAAAAGTA CCAGCATCGG AGGGGATATG TACACCCTGG AGAAGAACGA CGGAAGCCAG
    TCTGACACAG CCGTGGGCAC TGTGGGTGCG GGTGGAAAGA AGAGACGTTC AAGCATAGGC
    GCCAAAATGG TGGCCATTGT GGGCTTGTCA CGAAAAAGCC GAAGTACCTC ACAGCTCAGC
    CAAACAGAAG TCGGAGGGAA GAAGCTGAAG AGCACCATCC AGAGAAGTAC GGAAACAGGC
    CTGGCCGTGG AAATGAGAAA CTGGATGAGC CGCCAAGCAA GCAGGGAGTC CACAGATGGG
    AGTATGAACA GCTACAGCTC AGAGGGGAAT CTGATATTCC CTGGGGTCCG ACTGGGTTCC
    GACAGCCAGT TCAGTGACTT TTTGGATGGT CTTGGTCCTG CCCAGCTGGT TGGTCGCCAG
    ACTTTGGCCA CGCCCTCTAT GGGAGATATT CAGGTTGGAA TGGCTGACAA AAAAGGCCAG
    TTGGAGGTGG AGGTGATACG AGCCCGTGGT CTTGTTGTAA AACCAGGCTC AAAGACACTG
    CCAGCACCCT ATGTTAAGGT GTATCTCTTA GAAAATGGAG TGTGCATAGC CAAAAAGAAA
    ACAAAAGTAG CAAGAAAAAC GCTGGAGCCT CTATACCAGC AAGTGTTAGC GTTTGAGGAA
    TCACCACAAG GGAAGGTCCT ACAGATCATT GTTTGGGGTG ACTATGGACG CATGGACCAC
    AAGTCCTTCA TGGGGGTGGC TCAGATACTT TTAGATGAAC TGGACCTATC CAGCATGGTG
    ATTGGATGGT ATAAACTTTT CCCCCCTTCA TCTTTAGTAG ACCCAACGTT GGCTCCACTC
    ACTAGGAGAG CTTCCCAATC ATCTCTTGAA AGCTCCACGG GACCTTCTTA TGCTCGTTCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa59900
    Clone ID Related Accession (Same CDS sequence) XM_031903559.1
    Accession Version XM_031903559.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5148bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 2 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCTGCTC CACTAGGGCC CAGGGGCGGC CCTCTGCCTC CTGCACAGCC ACAGGCTCCA 
    ATGCCAGAGA TGCCGGACCT CAGCCACCTG ACAGAGGATG AGAGGAAAAT CATAATGGCT
    GTGATGGAGA GGCAGAAGAA AGAAGAGGAG AAGGAGCAGT CAGTGTTAAA GGTCAAAGAA
    GAACAAAAAG CCCAGCCGGC ACAGTGGTTT CCCTTTAGTG GGATCACTGA GCTGGTTAAT
    AACGTTCTTC AGCCACAGCC AAAAGAGCAA AATGAAAAGG AGCCGGAGAT AAAATTGCAC
    CAGCAGTTTG AAATGTACAA GGAGCAAGTG AAGAAGATGG GCGAGGAAGC ACAGCAGCAG
    CAGGAGCAGA AGGGCGAAGC TCCAACCTGT GGAATCTGCC ACAAAACAAA GTTTGCTGAT
    GGCTGTGGCC ACGACTGTTC GTATTGCCAA ACTAAATTCT GCGCGCGGTG TGGAGGGCGA
    GTGTCTCTGC GCTCAAATAA GGAGGACAAA GTGGTAATGT GGGTATGTAA TTTGTGCCGA
    AAACAACAAG AAATCCTCAC CAAATCAGGG GCCTGGTTTT ACAGCAGTGG GTCAAGCACA
    CCCCTAAAGC CCGGCGAAGA GAGCCAGGCA GGGTACCAAA CGGAAGAAGC ACCTCAGGAG
    AAAAAAGCTA AGCTACAAGA GCAGTCCCAA TACCAGGGAC CCTCAGGGGA CTTATTAACA
    CCAGCCTCGG ACAAAAACCG ACCGCAAGGG ATCGTCCGGC AAGATTCCAT CAAAAATGGT
    TCCGAACTGA AGCACCCGAT TCCTGGCGAC CTCGCATCTG ACAGGAAAAG AAGCCCTTCA
    ATACCTAGAG ACCAGAATCG GAGATATGAT ACAAGGGACG ATATAGGTGA ACATTCACAG
    CATGCTACAT CAGACAGCGC CATGCCTAGA TCACCATCAG ACTACTCGGA CAGGCGATTG
    CAACGAGGGT CTCAGCTCTA TGATGAGCCT GAGCACGGTG ACTACAGAAG AAGTAGGCGG
    CGCTCCAATG AGTATTCGGT AGATGAGGAG GATATCCAAG ACAGGGAGGA ATATGAAAGG
    CAGAGAAGAG AGGAGGAGTA CCAGGCAAGG TACCGGAGTG ACCCCAACTT GGCCAGGTAC
    CCTGTTAAGC CACAACCGTA TGAAGAGCAA ATGCGCATCC ATGCAGAGGT TTCAAGGGCT
    CGACAACGAA GGCACAGCGA TGTGTCTTTA GCTCAGACAG AATTTGAGGA GTCTCGGATG
    TCAACGCTGA GGATGGAGAG ACCTTCAAGG CAAAGATCTG CGTCAGAGCG TAGAGCAGCG
    ATGGAAAGCC AGCGTTCATA TTCAATAGAA AGAACTAGAG ATGTGCAAGG GCCAGGTCCT
    GGTCGGCAAA GGACCTCAAA TCATAGTCCC CCAACACCAA GAAGGAGTCC CATTCCCAAT
    GATAGGCCAG ATATGAGGCG CACAGATTCT TTACGGAAAC AGCACCACTT GGACCCAAGT
    TCTGCCGTAA GGAAAACCAA AAGGGAAAAA ATGGAAACTA TGTTAAGGAA TGATTCTCTT
    AGCTCAGATC AGTCTGAATC TGTCCGGCCC CCACCACCAA AGCCTCATAA AACAAAAAAG
    GGAGGTAAAA TGCGTCAGGT TTCCTTGAGC AGTTCAGAAG AAGAGTTGGC GTCTACGCCG
    GAGTACACGA GCTGCGATGA TGTGGAGATT GAAAGTGAAA GTGTTAGTGA GAAAGGTGAC
    ATGGACTACA ACTGGGTGGA GCATTCGTCT TGGCACAGCA GTGAGGCCTC CCCAATGTCT
    TTGCACCCTG TCACGTGGCA ACCTTCCAAA GATGGAGACC GCTTAATTGG TCGCATATTA
    TTAAACAAGC GGCTCAAAGA TGGGAGCGTC CCGCGAGATT CTGGAGCCAT GCTGGGGCTG
    AAGGTCGTTG GAGGGAAAAT GACAGAATCA GGTCGACTTT GTGCGTTTAT CACCAAAGTG
    AAGAAGGGAA GCCTGGCAGA TACAGTAGGC CATCTTCGAC CAGGTGATGA GGTTTTAGAA
    TGGAGTGGGC GGATTTTGCA AGGAGCGACG TTCGAAGAAG TTTACAATAT TATACTGGAG
    TCCAAACCCG AGGCACAAGT AGAATTATTG GTCTCCAGAC CCATAGGAGA TATACCCCGG
    ATTCCTGACA GCACACACGC ACAACTGGAA TCCAGTTCTA GCTCATTTGA ATCCCAGAAA
    ATGGATCGCC CTTCTATTTC AGTTACTTCC CCCATGAGTC CTGGAATGTT ACGGGATGTC
    CCCCAGTTTC TTTCTGGACA ACTTTCAAGC CAAAGCCTTA ATAGAAGAAC AGCGCCTTTT
    GTTCCTAGGG TTCAGATTAA ACTCTGGTAT GACAAGGTTG GCCACCAGTT AATTGTCACG
    ATATTAGGAG CAAAGGATCT GCCATCGCGG GAAGATGGGA GGCCGAGAAA TCCTTACGTA
    AAAATTTACT TTCTTCCAGA TCGAAGTGAT AAGAACAAAA GGAGAACAAA GACTGTGAAG
    AAAACCGTAG AGCCCAAATG GAACCAAACA TTCATCTATT CTCCGGTTCA TCGGAGGGAA
    TTTCGGGAAC GAATGTTGGA AATAACACTT TGGGATCAAG CTCGGGTTCG AGAAGAAGAG
    AGTGAATTTT TAGGAGAGAT TCTAATTGAA TTAGAAACTG CACTATTAGA TGATGAGCCA
    CATTGGTACA AGCTACAGAC GCATGATGTG TCTTCATTGC CACTTCCACA CCCATCCCCG
    TTCATGCAAA GAAGGCAGCT GCACGGCGAG AGCCCAACCC GGAGGTTGCA AAATAAAGGC
    TCATATTCAT ATAATTCAGG ATCCAAAAGG ATAAGCGACA GCGAAGTGTC TGACTACGAC
    TGTGATGATG GCGTTGGTGT TGTTTCAGAC TTTCGGCATA ATGGGCGTGA CCTGCAGAGT
    TCAACGTTAT CTGTTCCGGA ACAAGTAATG TCGTCCAATC ACTGTTCCCG GACTGGGCCC
    CCGCATGTAG ACAGCATAGG AAGAACGAGG TCATGGTCTC CCAGTGTCCC TCCTCCGCAA
    AGTAGGAATT TGGAACAGGG ACCGAGAGGG ACAAGATCTG GCCCTGCACA TTACAATACC
    ATGAGCAGAG TGGAAAGGCA TCGAGTATTA GATAACCACT ACTCCCCAGA CAGAGACAGG
    AATTGTGAAG CGGCAGATAG ACAGCCATAT CAAAGATCCA GGTCCACTGA ACAACGGCCT
    GTGCTGGAGC GGACCAGCTC CCGCTCCCGA TCTACTGAGC GTCCTGACTC AAACCTCATG
    AGGTCGATGC CTTCATTAAT GACGGGAAGA TCTGCCCCTC CTTCACCCGC CTTATCGAGG
    GCACACCCTC CTCGTACCGG CTCTGTTCAG ACAAGTCCTT CAGGCACCCC GTTAGCAGGG
    CGGAGGGGGC GCCAGCTCCC GCAACTTCCC CCAAAAGGCA CGTTAGAAAG AAAAGTTACT
    TGGGAAGACC AGCAAAGAAA GGTGAATGGT TCAATAACTG ATGACAAACA TTTGCCAAGA
    AAGAAACAGC ATTCTGAGCC AGAAGATATG GACTCAACGC GGAGGCGAAA CTCAGAGGAA
    AAGGCTGCAG AACAAGAGAA TGGGAACCCA GGTGCTATGG ACGTAGAAGA AAGGACGCGA
    CAGCTGAAAG TGAAGATCAA CAAATACAAG CAGGTGGGAG GATCAGATTC TCGGCTAGAG
    AAGGATTATC ATCCAAAGTA TCGGACGGGG CGGGATCCGC AGAGGGGCTC AGACAATATG
    TCCAACAAGT CATCAGACAG CGATGTTAGT GATGTGTCTG CCGTATCACG GACCAGCAGC
    GCCTCTCGTT TTAGTAGTAC GAGTTATATG TCTGTGCAGT CGGAAAGGCC TCGAGGGAAC
    ATGAAAATAG GTAGAAGGAC AAGGGAAGTA GAGGAGAGAA TAGAGAGGGC TGAGGGAGGG
    CAGCATCACA AAGAAATAGG TTTTCTGGAG GAAGAGGAGG ACGTTAGGCA GGAAGTAGAT
    AGTGAGAAGG AAACAGAAGT GCAGCATATA ATTGACAACT GCATGAAGGG GCCAAGCAAA
    GAAAGTGTAG AGGAAGAGAG TCAACAGAAT GAGAAAGATG AGGAAAGCCA TGATGTTGAG
    GAAGCCCATA ATGAGAATCT GCCAAGGAGG TCCTCAGAAA ATGATATAAG TGCATTGGCA
    TCCAAAATGC AAAGCAGGCA AATGGGCGCC TCAGGGAAGA ACATGACCAA AAGTACCAGC
    ATCGGAGGGG ATATGTACAC CCTGGAGAAG AACGACGGAA GCCAGTCTGA CACAGCCGTG
    GGCACTGTGG GTGCGGGTGG AAAGAAGAGA CGTTCAAGCA TAGGCGCCAA AATGGTGGCC
    ATTGTGGGCT TGTCACGAAA AAGCCGAAGT ACCTCACAGC TCAGCCAAAC AGAAGTCGGA
    GGGAAGAAGC TGAAGAGCAC CATCCAGAGA AGTACGGAAA CAGGCCTGGC CGTGGAAATG
    AGAAACTGGA TGAGCCGCCA AGCAAGCAGG GAGTCCACAG ATGGGAGTAT GAACAGCTAC
    AGCTCAGAGG GGAATCTGAT ATTCCCTGGG GTCCGACTGG GTTCCGACAG CCAGTTCAGT
    GACTTTTTGG ATGGTCTTGG TCCTGCCCAG CTGGTTGGTC GCCAGACTTT GGCCACGCCC
    TCTATGGGAG ATATTCAGGT TGGAATGGCT GACAAAAAAG GCCAGTTGGA GGTGGAGGTG
    ATACGAGCCC GTGGTCTTGT TGTAAAACCA GGCTCAAAGA CACTGCCAGC ACCCTATGTT
    AAGGTGTATC TCTTAGAAAA TGGAGTGTGC ATAGCCAAAA AGAAAACAAA AGTAGCAAGA
    AAAACGCTGG AGCCTCTATA CCAGCAAGTG TTAGCGTTTG AGGAATCACC ACAAGGGAAG
    GTCCTACAGA TCATTGTTTG GGGTGACTAT GGACGCATGG ACCACAAGTC CTTCATGGGG
    GTGGCTCAGA TACTTTTAGA TGAACTGGAC CTATCCAGCA TGGTGATTGG ATGGTATAAA
    CTTTTCCCCC CTTCATCTTT AGTAGACCCA ACGTTGGCTC CACTCACTAG GAGAGCTTCC
    CAATCATCTC TTGAAAGCTC CACGGGACCT TCTTATGCTC GTTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031759419.1
    CDS421..5568
    Translation

    Target ORF information:

    RefSeq Version XM_031903559.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 2 (rims2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031903559.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCTGCTC CACTAGGGCC CAGGGGCGGC CCTCTGCCTC CTGCACAGCC ACAGGCTCCA 
    ATGCCAGAGA TGCCGGACCT CAGCCACCTG ACAGAGGATG AGAGGAAAAT CATAATGGCT
    GTGATGGAGA GGCAGAAGAA AGAAGAGGAG AAGGAGCAGT CAGTGTTAAA GGTCAAAGAA
    GAACAAAAAG CCCAGCCGGC ACAGTGGTTT CCCTTTAGTG GGATCACTGA GCTGGTTAAT
    AACGTTCTTC AGCCACAGCC AAAAGAGCAA AATGAAAAGG AGCCGGAGAT AAAATTGCAC
    CAGCAGTTTG AAATGTACAA GGAGCAAGTG AAGAAGATGG GCGAGGAAGC ACAGCAGCAG
    CAGGAGCAGA AGGGCGAAGC TCCAACCTGT GGAATCTGCC ACAAAACAAA GTTTGCTGAT
    GGCTGTGGCC ACGACTGTTC GTATTGCCAA ACTAAATTCT GCGCGCGGTG TGGAGGGCGA
    GTGTCTCTGC GCTCAAATAA GGAGGACAAA GTGGTAATGT GGGTATGTAA TTTGTGCCGA
    AAACAACAAG AAATCCTCAC CAAATCAGGG GCCTGGTTTT ACAGCAGTGG GTCAAGCACA
    CCCCTAAAGC CCGGCGAAGA GAGCCAGGCA GGGTACCAAA CGGAAGAAGC ACCTCAGGAG
    AAAAAAGCTA AGCTACAAGA GCAGTCCCAA TACCAGGGAC CCTCAGGGGA CTTATTAACA
    CCAGCCTCGG ACAAAAACCG ACCGCAAGGG ATCGTCCGGC AAGATTCCAT CAAAAATGGT
    TCCGAACTGA AGCACCCGAT TCCTGGCGAC CTCGCATCTG ACAGGAAAAG AAGCCCTTCA
    ATACCTAGAG ACCAGAATCG GAGATATGAT ACAAGGGACG ATATAGGTGA ACATTCACAG
    CATGCTACAT CAGACAGCGC CATGCCTAGA TCACCATCAG ACTACTCGGA CAGGCGATTG
    CAACGAGGGT CTCAGCTCTA TGATGAGCCT GAGCACGGTG ACTACAGAAG AAGTAGGCGG
    CGCTCCAATG AGTATTCGGT AGATGAGGAG GATATCCAAG ACAGGGAGGA ATATGAAAGG
    CAGAGAAGAG AGGAGGAGTA CCAGGCAAGG TACCGGAGTG ACCCCAACTT GGCCAGGTAC
    CCTGTTAAGC CACAACCGTA TGAAGAGCAA ATGCGCATCC ATGCAGAGGT TTCAAGGGCT
    CGACAACGAA GGCACAGCGA TGTGTCTTTA GCTCAGACAG AATTTGAGGA GTCTCGGATG
    TCAACGCTGA GGATGGAGAG ACCTTCAAGG CAAAGATCTG CGTCAGAGCG TAGAGCAGCG
    ATGGAAAGCC AGCGTTCATA TTCAATAGAA AGAACTAGAG ATGTGCAAGG GCCAGGTCCT
    GGTCGGCAAA GGACCTCAAA TCATAGTCCC CCAACACCAA GAAGGAGTCC CATTCCCAAT
    GATAGGCCAG ATATGAGGCG CACAGATTCT TTACGGAAAC AGCACCACTT GGACCCAAGT
    TCTGCCGTAA GGAAAACCAA AAGGGAAAAA ATGGAAACTA TGTTAAGGAA TGATTCTCTT
    AGCTCAGATC AGTCTGAATC TGTCCGGCCC CCACCACCAA AGCCTCATAA AACAAAAAAG
    GGAGGTAAAA TGCGTCAGGT TTCCTTGAGC AGTTCAGAAG AAGAGTTGGC GTCTACGCCG
    GAGTACACGA GCTGCGATGA TGTGGAGATT GAAAGTGAAA GTGTTAGTGA GAAAGGTGAC
    ATGGACTACA ACTGGGTGGA GCATTCGTCT TGGCACAGCA GTGAGGCCTC CCCAATGTCT
    TTGCACCCTG TCACGTGGCA ACCTTCCAAA GATGGAGACC GCTTAATTGG TCGCATATTA
    TTAAACAAGC GGCTCAAAGA TGGGAGCGTC CCGCGAGATT CTGGAGCCAT GCTGGGGCTG
    AAGGTCGTTG GAGGGAAAAT GACAGAATCA GGTCGACTTT GTGCGTTTAT CACCAAAGTG
    AAGAAGGGAA GCCTGGCAGA TACAGTAGGC CATCTTCGAC CAGGTGATGA GGTTTTAGAA
    TGGAGTGGGC GGATTTTGCA AGGAGCGACG TTCGAAGAAG TTTACAATAT TATACTGGAG
    TCCAAACCCG AGGCACAAGT AGAATTATTG GTCTCCAGAC CCATAGGAGA TATACCCCGG
    ATTCCTGACA GCACACACGC ACAACTGGAA TCCAGTTCTA GCTCATTTGA ATCCCAGAAA
    ATGGATCGCC CTTCTATTTC AGTTACTTCC CCCATGAGTC CTGGAATGTT ACGGGATGTC
    CCCCAGTTTC TTTCTGGACA ACTTTCAAGC CAAAGCCTTA ATAGAAGAAC AGCGCCTTTT
    GTTCCTAGGG TTCAGATTAA ACTCTGGTAT GACAAGGTTG GCCACCAGTT AATTGTCACG
    ATATTAGGAG CAAAGGATCT GCCATCGCGG GAAGATGGGA GGCCGAGAAA TCCTTACGTA
    AAAATTTACT TTCTTCCAGA TCGAAGTGAT AAGAACAAAA GGAGAACAAA GACTGTGAAG
    AAAACCGTAG AGCCCAAATG GAACCAAACA TTCATCTATT CTCCGGTTCA TCGGAGGGAA
    TTTCGGGAAC GAATGTTGGA AATAACACTT TGGGATCAAG CTCGGGTTCG AGAAGAAGAG
    AGTGAATTTT TAGGAGAGAT TCTAATTGAA TTAGAAACTG CACTATTAGA TGATGAGCCA
    CATTGGTACA AGCTACAGAC GCATGATGTG TCTTCATTGC CACTTCCACA CCCATCCCCG
    TTCATGCAAA GAAGGCAGCT GCACGGCGAG AGCCCAACCC GGAGGTTGCA AAATAAAGGC
    TCATATTCAT ATAATTCAGG ATCCAAAAGG ATAAGCGACA GCGAAGTGTC TGACTACGAC
    TGTGATGATG GCGTTGGTGT TGTTTCAGAC TTTCGGCATA ATGGGCGTGA CCTGCAGAGT
    TCAACGTTAT CTGTTCCGGA ACAAGTAATG TCGTCCAATC ACTGTTCCCG GACTGGGCCC
    CCGCATGTAG ACAGCATAGG AAGAACGAGG TCATGGTCTC CCAGTGTCCC TCCTCCGCAA
    AGTAGGAATT TGGAACAGGG ACCGAGAGGG ACAAGATCTG GCCCTGCACA TTACAATACC
    ATGAGCAGAG TGGAAAGGCA TCGAGTATTA GATAACCACT ACTCCCCAGA CAGAGACAGG
    AATTGTGAAG CGGCAGATAG ACAGCCATAT CAAAGATCCA GGTCCACTGA ACAACGGCCT
    GTGCTGGAGC GGACCAGCTC CCGCTCCCGA TCTACTGAGC GTCCTGACTC AAACCTCATG
    AGGTCGATGC CTTCATTAAT GACGGGAAGA TCTGCCCCTC CTTCACCCGC CTTATCGAGG
    GCACACCCTC CTCGTACCGG CTCTGTTCAG ACAAGTCCTT CAGGCACCCC GTTAGCAGGG
    CGGAGGGGGC GCCAGCTCCC GCAACTTCCC CCAAAAGGCA CGTTAGAAAG AAAAGTTACT
    TGGGAAGACC AGCAAAGAAA GGTGAATGGT TCAATAACTG ATGACAAACA TTTGCCAAGA
    AAGAAACAGC ATTCTGAGCC AGAAGATATG GACTCAACGC GGAGGCGAAA CTCAGAGGAA
    AAGGCTGCAG AACAAGAGAA TGGGAACCCA GGTGCTATGG ACGTAGAAGA AAGGACGCGA
    CAGCTGAAAG TGAAGATCAA CAAATACAAG CAGGTGGGAG GATCAGATTC TCGGCTAGAG
    AAGGATTATC ATCCAAAGTA TCGGACGGGG CGGGATCCGC AGAGGGGCTC AGACAATATG
    TCCAACAAGT CATCAGACAG CGATGTTAGT GATGTGTCTG CCGTATCACG GACCAGCAGC
    GCCTCTCGTT TTAGTAGTAC GAGTTATATG TCTGTGCAGT CGGAAAGGCC TCGAGGGAAC
    ATGAAAATAG GTAGAAGGAC AAGGGAAGTA GAGGAGAGAA TAGAGAGGGC TGAGGGAGGG
    CAGCATCACA AAGAAATAGG TTTTCTGGAG GAAGAGGAGG ACGTTAGGCA GGAAGTAGAT
    AGTGAGAAGG AAACAGAAGT GCAGCATATA ATTGACAACT GCATGAAGGG GCCAAGCAAA
    GAAAGTGTAG AGGAAGAGAG TCAACAGAAT GAGAAAGATG AGGAAAGCCA TGATGTTGAG
    GAAGCCCATA ATGAGAATCT GCCAAGGAGG TCCTCAGAAA ATGATATAAG TGCATTGGCA
    TCCAAAATGC AAAGCAGGCA AATGGGCGCC TCAGGGAAGA ACATGACCAA AAGTACCAGC
    ATCGGAGGGG ATATGTACAC CCTGGAGAAG AACGACGGAA GCCAGTCTGA CACAGCCGTG
    GGCACTGTGG GTGCGGGTGG AAAGAAGAGA CGTTCAAGCA TAGGCGCCAA AATGGTGGCC
    ATTGTGGGCT TGTCACGAAA AAGCCGAAGT ACCTCACAGC TCAGCCAAAC AGAAGTCGGA
    GGGAAGAAGC TGAAGAGCAC CATCCAGAGA AGTACGGAAA CAGGCCTGGC CGTGGAAATG
    AGAAACTGGA TGAGCCGCCA AGCAAGCAGG GAGTCCACAG ATGGGAGTAT GAACAGCTAC
    AGCTCAGAGG GGAATCTGAT ATTCCCTGGG GTCCGACTGG GTTCCGACAG CCAGTTCAGT
    GACTTTTTGG ATGGTCTTGG TCCTGCCCAG CTGGTTGGTC GCCAGACTTT GGCCACGCCC
    TCTATGGGAG ATATTCAGGT TGGAATGGCT GACAAAAAAG GCCAGTTGGA GGTGGAGGTG
    ATACGAGCCC GTGGTCTTGT TGTAAAACCA GGCTCAAAGA CACTGCCAGC ACCCTATGTT
    AAGGTGTATC TCTTAGAAAA TGGAGTGTGC ATAGCCAAAA AGAAAACAAA AGTAGCAAGA
    AAAACGCTGG AGCCTCTATA CCAGCAAGTG TTAGCGTTTG AGGAATCACC ACAAGGGAAG
    GTCCTACAGA TCATTGTTTG GGGTGACTAT GGACGCATGG ACCACAAGTC CTTCATGGGG
    GTGGCTCAGA TACTTTTAGA TGAACTGGAC CTATCCAGCA TGGTGATTGG ATGGTATAAA
    CTTTTCCCCC CTTCATCTTT AGTAGACCCA ACGTTGGCTC CACTCACTAG GAGAGCTTCC
    CAATCATCTC TTGAAAGCTC CACGGGACCT TCTTATGCTC GTTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa59901
    Clone ID Related Accession (Same CDS sequence) XM_031903560.1
    Accession Version XM_031903560.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5130bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 2 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCTGCTC CACTAGGGCC CAGGGGCGGC CCTCTGCCTC CTGCACAGCC ACAGGCTCCA 
    ATGCCAGAGA TGCCGGACCT CAGCCACCTG ACAGAGGATG AGAGGAAAAT CATAATGGCT
    GTGATGGAGA GGCAGAAGAA AGAAGAGGAG AAGGAGCAGT CAGTGTTAAA GGTCAAAGAA
    GAACAAAAAG CCCAGCCGGC ACAGTGGTTT CCCTTTAGTG GGATCACTGA GCTGGTTAAT
    AACGTTCTTC AGCCACAGCC AAAAGAGCAA AATGAAAAGG AGCCGGAGAT AAAATTGCAC
    CAGCAGTTTG AAATGTACAA GGAGCAAGTG AAGAAGATGG GCGAGGAAGC ACAGCAGCAG
    CAGGAGCAGA AGGGCGAAGC TCCAACCTGT GGAATCTGCC ACAAAACAAA GTTTGCTGAT
    GGCTGTGGCC ACGACTGTTC GTATTGCCAA ACTAAATTCT GCGCGCGGTG TGGAGGGCGA
    GTGTCTCTGC GCTCAAATAA GGAGGACAAA GTGGTAATGT GGGTATGTAA TTTGTGCCGA
    AAACAACAAG AAATCCTCAC CAAATCAGGG GCCTGGTTTT ACAGCAGTGG GTCAAGCACA
    CCCCTAAAGC CCGGCGAAGA GAGCCAGGCA GGGTACCAAA CGGAAGAAGC ACCTCAGGAG
    AAAAAAGCTA AGCTACAAGA GCAGTCCCAA TACCAGGGAC CCTCAGGGGA CTTATTAACA
    CCAGCCTCGG ACAAAAACCG ACCGCAAGGG ATCGTCCGGC AAGATTCCAT CAAAAATGGT
    TCCGAACTGA AGCACCCGAT TCCTGGCGAC CTCGCATCTG ACAGGAAAAG AAGCCCTTCA
    ATACCTAGAG ACCAGAATCG GAGATATGAT ACAAGGGACG ATATAGGTGA ACATTCACAG
    CATGCTACAT CAGACAGCGC CATGCCTAGA TCACCATCAG ACTACTCGGA CAGGCGATTG
    CAACGAGGGT CTCAGCTCTA TGATGAGCCT GAGCACGGTG ACTACAGAAG AAGTAGGCGG
    CGCTCCAATG AGTATTCGGT AGATGAGGAG GATATCCAAG ACAGGGAGGA ATATGAAAGG
    CAGAGAAGAG AGGAGGAGTA CCAGGCAAGG TACCGGAGTG ACCCCAACTT GGCCAGGTAC
    CCTGTTAAGC CACAACCGTA TGAAGAGCAA ATGCGCATCC ATGCAGAGGT TTCAAGGGCT
    CGACAACGAA GGCACAGCGA TGTGTCTTTA GCTCAGACAG AATTTGAGGA GTCTCGGATG
    TCAACGCTGA GGATGGAGAG ACCTTCAAGG CAAAGATCTG CGTCAGAGCG TAGAGCAGCG
    ATGGAAAGCC AGCGTTCATA TTCAATAGAA AGAACTAGAG ATGTGCAAGG GCCAGGTCCT
    GGTCGGCAAA GGACCTCAAA TCATAGTCCC CCAACACCAA GAAGGAGTCC CATTCCCAAT
    GATAGGCCAG ATATGAGGCG CACAGATTCT TTACGGAAAC AGCACCACTT GGACCCAAGT
    TCTGCCGTAA GGAAAACCAA AAGGGAAAAA ATGGAAACTA TGTTAAGGAA TGATTCTCTT
    AGCTCAGATC AGTCTGAATC TGTCCGGCCC CCACCACCAA AGCCTCATAA AACAAAAAAG
    GGAGGTAAAA TGCGTCAGGT TTCCTTGAGC AGTTCAGAAG AAGAGTTGGC GTCTACGCCG
    GAGTACACGA GCTGCGATGA TGTGGAGATT GAAAGTGAAA GTGTTAGTGA GAAAGGGGAC
    AGTCAAAGGG GAAAAAGAAA AACTAGTGAG CAGGCAGTTT TGTCGGATTC TAACATCTTG
    TCTGAGAGAC AAAAGAAAAT GGTGTGCTTT GGAGGCCACT CTTTGGAAGA GGATTTGGAA
    TGGTCTGAGC CTCAGATTAA AGACTCTGGG GTAGACACGT GTAGTAGCAC CACCCTTAAC
    GAAGAGCATA GCCATAGCGA GAAGCACCCT GTCACGTGGC AACCTTCCAA AGATGGAGAC
    CGCTTAATTG GTCGCATATT ATTAAACAAG CGGCTCAAAG ATGGGAGCGT CCCGCGAGAT
    TCTGGAGCCA TGCTGGGGCT GAAGGTCGTT GGAGGGAAAA TGACAGAATC AGGTCGACTT
    TGTGCGTTTA TCACCAAAGT GAAGAAGGGA AGCCTGGCAG ATACAGTAGG CCATCTTCGA
    CCAGGTGATG AGGTTTTAGA ATGGAGTGGG CGGATTTTGC AAGGAGCGAC GTTCGAAGAA
    GTTTACAATA TTATACTGGA GTCCAAACCC GAGGCACAAG TAGAATTATT GGTCTCCAGA
    CCCATAGGAG ATATACCCCG GATTCCTGAC AGCACACACG CACAACTGGA ATCCAGTTCT
    AGCTCATTTG AATCCCAGAA AATGGATCGC CCTTCTATTT CAGTTACTTC CCCCATGAGT
    CCTGGAATGT TACGGGATGT CCCCCAGTTT CTTTCTGGAC AACTTTCAAG CCAAAGCCTT
    AATAGAAGAA CAGCGCCTTT TGTTCCTAGG GTTCAGATTA AACTCTGGTA TGACAAGGTT
    GGCCACCAGT TAATTGTCAC GATATTAGGA GCAAAGGATC TGCCATCGCG GGAAGATGGG
    AGGCCGAGAA ATCCTTACGT AAAAATTTAC TTTCTTCCAG ATCGAAGTGA TAAGAACAAA
    AGGAGAACAA AGACTGTGAA GAAAACCGTA GAGCCCAAAT GGAACCAAAC ATTCATCTAT
    TCTCCGGTTC ATCGGAGGGA ATTTCGGGAA CGAATGTTGG AAATAACACT TTGGGATCAA
    GCTCGGGTTC GAGAAGAAGA GAGTGAATTT TTAGGAGAGA TTCTAATTGA ATTAGAAACT
    GCACTATTAG ATGATGAGCC ACATTGGTAC AAGCTACAGA CGCATGATGT GTCTTCATTG
    CCACTTCCAC ACCCATCCCC GTTCATGCAA AGAAGGCAGC TGCACGGCGA GAGCCCAACC
    CGGAGGTTGC AAAATAAAGG CTCATATTCA TATAATTCAG GATCCAAAAG GATAAGCGAC
    AGCGAAGTGT CTGACTACGA CTGTGATGAT GGCGTTGGTG TTGTTTCAGA CTTTCGGCAT
    AATGGGCGTG ACCTGCAGAG TTCAACGTTA TCTGTTCCGG AACAAGTAAT GTCGTCCAAT
    CACTGTTCCC GGACTGGGCC CCCGCATGTA GACAGCATAG GAAGAACGAG GTCATGGTCT
    CCCAGTGTCC CTCCTCCGCA AAGTAGGAAT TTGGAACAGG GACCGAGAGG GACAAGATCT
    GGCCCTGCAC ATTACAATAC CATGAGCAGA GTGGAAAGGC ATCGAGTATT AGATAACCAC
    TACTCCCCAG ACAGAGACAG GAATTGTGAA GCGGCAGATA GACAGCCATA TCAAAGATCC
    AGGTCCACTG AACAACGGCC TGTGCTGGAG CGGACCAGCT CCCGCTCCCG ATCTACTGAG
    CGTCCTGACT CAAACCTCAT GAGGTCGATG CCTTCATTAA TGACGGGAAG ATCTGCCCCT
    CCTTCACCCG CCTTATCGAG GGCACACCCT CCTCGTACCG GCTCTGTTCA GACAAGTCCT
    TCAGGCACCC CGTTAGCAGG GCGGAGGGGG CGCCAGCTCC CGCAACTTCC CCCAAAAGGC
    ACGTTAGAAA GAAGTGCTAT GGACGTAGAA GAAAGGACGC GACAGCTGAA AGTGAAGATC
    AACAAATACA AGCAGGTGGG AGGATCAGAT TCTCGGCTAG AGAAGGATTA TCATCCAAAG
    TATCGGACGG GGCGGGATCC GCAGAGGGGC TCAGACAATA TGTCCAACAA GTCATCAGAC
    AGCGATGTTA GTGATGTGTC TGCCGTATCA CGGACCAGCA GCGCCTCTCG TTTTAGTAGT
    ACGAGTTATA TGTCTGTGCA GTCGGAAAGG CCTCGAGGGA ACATGAAAAT AGGTAGAAGG
    ACAAGGGAAG TAGAGGAGAG AATAGAGAGG GCTGAGGGAG GGCAGCATCA CAAAGAAATA
    GGTTTTCTGG AGGAAGAGGA GGACGTTAGG CAGGAAGTAG ATAGTGAGAA GGAAACAGAA
    GTGCAGCATA TAATTGACAA CTGCATGAAG GGGCCAAGCA AAGAAAGTGT AGAGGAAGAG
    AGTCAACAGA ATGAGAAAGA TGAGGAAAGC CATGATGTTG AGGAAGCCCA TAATGAGAAT
    CTGCCAAGGA GGTCCTCAGA AAATGATATA AGTGCATTGG CATCCAAAAT GCAAAGCAGG
    CAAATGGGCG CCTCAGGGAA GAACATGACC AAAAGTACCA GCATCGGAGG GGATATGTAC
    ACCCTGGAGA AGAACGACGG AAGCCAGTCT GACACAGCCG TGGGCACTGT GGGTGCGGGT
    GGAAAGAAGA GACGTTCAAG CATAGGCGCC AAAATGGTGG CCATTGTGGG CTTGTCACGA
    AAAAGCCGAA GTACCTCACA GCTCAGCCAA ACAGAAGTCG GAGGGAAGAA GCTGAAGAGC
    ACCATCCAGA GAAGTACGGA AACAGGCCTG GCCGTGGAAA TGAGAAACTG GATGAGCCGC
    CAAGCAAGCA GGGAGTCCAC AGATGGGAGT ATGAACAGCT ACAGCTCAGA GGGGAATCTG
    ATATTCCCTG GGGTCCGACT GGGTTCCGAC AGCCAGTTCA GTGACTTTTT GGATGGTCTT
    GGTCCTGCCC AGCTGGTTGG TCGCCAGACT TTGGCCACGC CCTCTATGGG AGATATTCAG
    GTTGGAATGG CTGACAAAAA AGGCCAGTTG GAGGTGGAGG TGATACGAGC CCGTGGTCTT
    GTTGTAAAAC CAGGCTCAAA GACACTGCCA GCACCCTATG TTAAGGTGTA TCTCTTAGAA
    AATGGAGTGT GCATAGCCAA AAAGAAAACA AAAGTAGCAA GAAAAACGCT GGAGCCTCTA
    TACCAGCAAG TGTTAGCGTT TGAGGAATCA CCACAAGGGA AGGTCCTACA GATCATTGTT
    TGGGGTGACT ATGGACGCAT GGACCACAAG TCCTTCATGG GGGTGGCTCA GATACTTTTA
    GATGAACTGG ACCTATCCAG CATGGTGATT GGATGGTATA AACTTTTCCC CCCTTCATCT
    TTAGTAGACC CAACGTTGGC TCCACTCACT AGGAGAGCTT CCCAATCATC TCTTGAAAGC
    TCCACGGGAC CTTCTTATGC TCGTTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031759420.1
    CDS421..5550
    Translation

    Target ORF information:

    RefSeq Version XM_031903560.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 2 (rims2), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031903560.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCTGCTC CACTAGGGCC CAGGGGCGGC CCTCTGCCTC CTGCACAGCC ACAGGCTCCA 
    ATGCCAGAGA TGCCGGACCT CAGCCACCTG ACAGAGGATG AGAGGAAAAT CATAATGGCT
    GTGATGGAGA GGCAGAAGAA AGAAGAGGAG AAGGAGCAGT CAGTGTTAAA GGTCAAAGAA
    GAACAAAAAG CCCAGCCGGC ACAGTGGTTT CCCTTTAGTG GGATCACTGA GCTGGTTAAT
    AACGTTCTTC AGCCACAGCC AAAAGAGCAA AATGAAAAGG AGCCGGAGAT AAAATTGCAC
    CAGCAGTTTG AAATGTACAA GGAGCAAGTG AAGAAGATGG GCGAGGAAGC ACAGCAGCAG
    CAGGAGCAGA AGGGCGAAGC TCCAACCTGT GGAATCTGCC ACAAAACAAA GTTTGCTGAT
    GGCTGTGGCC ACGACTGTTC GTATTGCCAA ACTAAATTCT GCGCGCGGTG TGGAGGGCGA
    GTGTCTCTGC GCTCAAATAA GGAGGACAAA GTGGTAATGT GGGTATGTAA TTTGTGCCGA
    AAACAACAAG AAATCCTCAC CAAATCAGGG GCCTGGTTTT ACAGCAGTGG GTCAAGCACA
    CCCCTAAAGC CCGGCGAAGA GAGCCAGGCA GGGTACCAAA CGGAAGAAGC ACCTCAGGAG
    AAAAAAGCTA AGCTACAAGA GCAGTCCCAA TACCAGGGAC CCTCAGGGGA CTTATTAACA
    CCAGCCTCGG ACAAAAACCG ACCGCAAGGG ATCGTCCGGC AAGATTCCAT CAAAAATGGT
    TCCGAACTGA AGCACCCGAT TCCTGGCGAC CTCGCATCTG ACAGGAAAAG AAGCCCTTCA
    ATACCTAGAG ACCAGAATCG GAGATATGAT ACAAGGGACG ATATAGGTGA ACATTCACAG
    CATGCTACAT CAGACAGCGC CATGCCTAGA TCACCATCAG ACTACTCGGA CAGGCGATTG
    CAACGAGGGT CTCAGCTCTA TGATGAGCCT GAGCACGGTG ACTACAGAAG AAGTAGGCGG
    CGCTCCAATG AGTATTCGGT AGATGAGGAG GATATCCAAG ACAGGGAGGA ATATGAAAGG
    CAGAGAAGAG AGGAGGAGTA CCAGGCAAGG TACCGGAGTG ACCCCAACTT GGCCAGGTAC
    CCTGTTAAGC CACAACCGTA TGAAGAGCAA ATGCGCATCC ATGCAGAGGT TTCAAGGGCT
    CGACAACGAA GGCACAGCGA TGTGTCTTTA GCTCAGACAG AATTTGAGGA GTCTCGGATG
    TCAACGCTGA GGATGGAGAG ACCTTCAAGG CAAAGATCTG CGTCAGAGCG TAGAGCAGCG
    ATGGAAAGCC AGCGTTCATA TTCAATAGAA AGAACTAGAG ATGTGCAAGG GCCAGGTCCT
    GGTCGGCAAA GGACCTCAAA TCATAGTCCC CCAACACCAA GAAGGAGTCC CATTCCCAAT
    GATAGGCCAG ATATGAGGCG CACAGATTCT TTACGGAAAC AGCACCACTT GGACCCAAGT
    TCTGCCGTAA GGAAAACCAA AAGGGAAAAA ATGGAAACTA TGTTAAGGAA TGATTCTCTT
    AGCTCAGATC AGTCTGAATC TGTCCGGCCC CCACCACCAA AGCCTCATAA AACAAAAAAG
    GGAGGTAAAA TGCGTCAGGT TTCCTTGAGC AGTTCAGAAG AAGAGTTGGC GTCTACGCCG
    GAGTACACGA GCTGCGATGA TGTGGAGATT GAAAGTGAAA GTGTTAGTGA GAAAGGGGAC
    AGTCAAAGGG GAAAAAGAAA AACTAGTGAG CAGGCAGTTT TGTCGGATTC TAACATCTTG
    TCTGAGAGAC AAAAGAAAAT GGTGTGCTTT GGAGGCCACT CTTTGGAAGA GGATTTGGAA
    TGGTCTGAGC CTCAGATTAA AGACTCTGGG GTAGACACGT GTAGTAGCAC CACCCTTAAC
    GAAGAGCATA GCCATAGCGA GAAGCACCCT GTCACGTGGC AACCTTCCAA AGATGGAGAC
    CGCTTAATTG GTCGCATATT ATTAAACAAG CGGCTCAAAG ATGGGAGCGT CCCGCGAGAT
    TCTGGAGCCA TGCTGGGGCT GAAGGTCGTT GGAGGGAAAA TGACAGAATC AGGTCGACTT
    TGTGCGTTTA TCACCAAAGT GAAGAAGGGA AGCCTGGCAG ATACAGTAGG CCATCTTCGA
    CCAGGTGATG AGGTTTTAGA ATGGAGTGGG CGGATTTTGC AAGGAGCGAC GTTCGAAGAA
    GTTTACAATA TTATACTGGA GTCCAAACCC GAGGCACAAG TAGAATTATT GGTCTCCAGA
    CCCATAGGAG ATATACCCCG GATTCCTGAC AGCACACACG CACAACTGGA ATCCAGTTCT
    AGCTCATTTG AATCCCAGAA AATGGATCGC CCTTCTATTT CAGTTACTTC CCCCATGAGT
    CCTGGAATGT TACGGGATGT CCCCCAGTTT CTTTCTGGAC AACTTTCAAG CCAAAGCCTT
    AATAGAAGAA CAGCGCCTTT TGTTCCTAGG GTTCAGATTA AACTCTGGTA TGACAAGGTT
    GGCCACCAGT TAATTGTCAC GATATTAGGA GCAAAGGATC TGCCATCGCG GGAAGATGGG
    AGGCCGAGAA ATCCTTACGT AAAAATTTAC TTTCTTCCAG ATCGAAGTGA TAAGAACAAA
    AGGAGAACAA AGACTGTGAA GAAAACCGTA GAGCCCAAAT GGAACCAAAC ATTCATCTAT
    TCTCCGGTTC ATCGGAGGGA ATTTCGGGAA CGAATGTTGG AAATAACACT TTGGGATCAA
    GCTCGGGTTC GAGAAGAAGA GAGTGAATTT TTAGGAGAGA TTCTAATTGA ATTAGAAACT
    GCACTATTAG ATGATGAGCC ACATTGGTAC AAGCTACAGA CGCATGATGT GTCTTCATTG
    CCACTTCCAC ACCCATCCCC GTTCATGCAA AGAAGGCAGC TGCACGGCGA GAGCCCAACC
    CGGAGGTTGC AAAATAAAGG CTCATATTCA TATAATTCAG GATCCAAAAG GATAAGCGAC
    AGCGAAGTGT CTGACTACGA CTGTGATGAT GGCGTTGGTG TTGTTTCAGA CTTTCGGCAT
    AATGGGCGTG ACCTGCAGAG TTCAACGTTA TCTGTTCCGG AACAAGTAAT GTCGTCCAAT
    CACTGTTCCC GGACTGGGCC CCCGCATGTA GACAGCATAG GAAGAACGAG GTCATGGTCT
    CCCAGTGTCC CTCCTCCGCA AAGTAGGAAT TTGGAACAGG GACCGAGAGG GACAAGATCT
    GGCCCTGCAC ATTACAATAC CATGAGCAGA GTGGAAAGGC ATCGAGTATT AGATAACCAC
    TACTCCCCAG ACAGAGACAG GAATTGTGAA GCGGCAGATA GACAGCCATA TCAAAGATCC
    AGGTCCACTG AACAACGGCC TGTGCTGGAG CGGACCAGCT CCCGCTCCCG ATCTACTGAG
    CGTCCTGACT CAAACCTCAT GAGGTCGATG CCTTCATTAA TGACGGGAAG ATCTGCCCCT
    CCTTCACCCG CCTTATCGAG GGCACACCCT CCTCGTACCG GCTCTGTTCA GACAAGTCCT
    TCAGGCACCC CGTTAGCAGG GCGGAGGGGG CGCCAGCTCC CGCAACTTCC CCCAAAAGGC
    ACGTTAGAAA GAAGTGCTAT GGACGTAGAA GAAAGGACGC GACAGCTGAA AGTGAAGATC
    AACAAATACA AGCAGGTGGG AGGATCAGAT TCTCGGCTAG AGAAGGATTA TCATCCAAAG
    TATCGGACGG GGCGGGATCC GCAGAGGGGC TCAGACAATA TGTCCAACAA GTCATCAGAC
    AGCGATGTTA GTGATGTGTC TGCCGTATCA CGGACCAGCA GCGCCTCTCG TTTTAGTAGT
    ACGAGTTATA TGTCTGTGCA GTCGGAAAGG CCTCGAGGGA ACATGAAAAT AGGTAGAAGG
    ACAAGGGAAG TAGAGGAGAG AATAGAGAGG GCTGAGGGAG GGCAGCATCA CAAAGAAATA
    GGTTTTCTGG AGGAAGAGGA GGACGTTAGG CAGGAAGTAG ATAGTGAGAA GGAAACAGAA
    GTGCAGCATA TAATTGACAA CTGCATGAAG GGGCCAAGCA AAGAAAGTGT AGAGGAAGAG
    AGTCAACAGA ATGAGAAAGA TGAGGAAAGC CATGATGTTG AGGAAGCCCA TAATGAGAAT
    CTGCCAAGGA GGTCCTCAGA AAATGATATA AGTGCATTGG CATCCAAAAT GCAAAGCAGG
    CAAATGGGCG CCTCAGGGAA GAACATGACC AAAAGTACCA GCATCGGAGG GGATATGTAC
    ACCCTGGAGA AGAACGACGG AAGCCAGTCT GACACAGCCG TGGGCACTGT GGGTGCGGGT
    GGAAAGAAGA GACGTTCAAG CATAGGCGCC AAAATGGTGG CCATTGTGGG CTTGTCACGA
    AAAAGCCGAA GTACCTCACA GCTCAGCCAA ACAGAAGTCG GAGGGAAGAA GCTGAAGAGC
    ACCATCCAGA GAAGTACGGA AACAGGCCTG GCCGTGGAAA TGAGAAACTG GATGAGCCGC
    CAAGCAAGCA GGGAGTCCAC AGATGGGAGT ATGAACAGCT ACAGCTCAGA GGGGAATCTG
    ATATTCCCTG GGGTCCGACT GGGTTCCGAC AGCCAGTTCA GTGACTTTTT GGATGGTCTT
    GGTCCTGCCC AGCTGGTTGG TCGCCAGACT TTGGCCACGC CCTCTATGGG AGATATTCAG
    GTTGGAATGG CTGACAAAAA AGGCCAGTTG GAGGTGGAGG TGATACGAGC CCGTGGTCTT
    GTTGTAAAAC CAGGCTCAAA GACACTGCCA GCACCCTATG TTAAGGTGTA TCTCTTAGAA
    AATGGAGTGT GCATAGCCAA AAAGAAAACA AAAGTAGCAA GAAAAACGCT GGAGCCTCTA
    TACCAGCAAG TGTTAGCGTT TGAGGAATCA CCACAAGGGA AGGTCCTACA GATCATTGTT
    TGGGGTGACT ATGGACGCAT GGACCACAAG TCCTTCATGG GGGTGGCTCA GATACTTTTA
    GATGAACTGG ACCTATCCAG CATGGTGATT GGATGGTATA AACTTTTCCC CCCTTCATCT
    TTAGTAGACC CAACGTTGGC TCCACTCACT AGGAGAGCTT CCCAATCATC TCTTGAAAGC
    TCCACGGGAC CTTCTTATGC TCGTTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa59904
    Clone ID Related Accession (Same CDS sequence) XM_031903563.1
    Accession Version XM_031903563.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5010bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 2 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTCTGCTC CACTAGGGCC CAGGGGCGGC CCTCTGCCTC CTGCACAGCC ACAGGCTCCA 
    ATGCCAGAGA TGCCGGACCT CAGCCACCTG ACAGAGGATG AGAGGAAAAT CATAATGGCT
    GTGATGGAGA GGCAGAAGAA AGAAGAGGAG AAGGAGCAGT CAGTGTTAAA GGTCAAAGAA
    GAACAAAAAG CCCAGCCGGC ACAGTGGTTT CCCTTTAGTG GGATCACTGA GCTGGTTAAT
    AACGTTCTTC AGCCACAGCC AAAAGAGCAA AATGAAAAGG AGCCGGAGAT AAAATTGCAC
    CAGCAGTTTG AAATGTACAA GGAGCAAGTG AAGAAGATGG GCGAGGAAGC ACAGCAGCAG
    CAGGAGCAGA AGGGCGAAGC TCCAACCTGT GGAATCTGCC ACAAAACAAA GTTTGCTGAT
    GGCTGTGGCC ACGACTGTTC GTATTGCCAA ACTAAATTCT GCGCGCGGTG TGGAGGGCGA
    GTGTCTCTGC GCTCAAATAA GGAGGACAAA GTGGTAATGT GGGTATGTAA TTTGTGCCGA
    AAACAACAAG AAATCCTCAC CAAATCAGGG GCCTGGTTTT ACAGCAGTGG GTCAAGCACA
    CCCCTAAAGC CCGGCGAAGA GAGCCAGGCA GGGTACCAAA CGGAAGAAGC ACCTCAGGAG
    AAAAAAGCTA AGCTACAAGA GCAGTCCCAA TACCAGGGAC CCTCAGGGGA CTTATTAACA
    CCAGCCTCGG ACAAAAACCG ACCGCAAGGG ATCGTCCGGC AAGATTCCAT CAAAAATGGT
    TCCGAACTGA AGCACCCGAT TCCTGGCGAC CTCGCATCTG ACAGGAAAAG AAGCCCTTCA
    ATACCTAGAG ACCAGAATCG GAGATATGAT ACAAGGGACG ATATAGGTGA ACATTCACAG
    CATGCTACAT CAGACAGCGC CATGCCTAGA TCACCATCAG ACTACTCGGA CAGGCGATTG
    CAACGAGGGT CTCAGCTCTA TGATGAGCCT GAGCACGGTG ACTACAGAAG AAGTAGGCGG
    CGCTCCAATG AGTATTCGGT AGATGAGGAG GATATCCAAG ACAGGGAGGA ATATGAAAGG
    CAGAGAAGAG AGGAGGAGTA CCAGGCAAGG TACCGGAGTG ACCCCAACTT GGCCAGGTAC
    CCTGTTAAGC CACAACCGTA TGAAGAGCAA ATGCGCATCC ATGCAGAGGT TTCAAGGGCT
    CGACAACGAA GGCACAGCGA TGTGTCTTTA GCTCAGACAG AATTTGAGGA GTCTCGGATG
    TCAACGCTGA GGATGGAGAG ACCTTCAAGG CAAAGATCTG CGTCAGAGCG TAGAGCAGCG
    ATGGAAAGCC AGCGTTCATA TTCAATAGAA AGAACTAGAG ATGTGCAAGG GCCAGGTCCT
    GGTCGGCAAA GGACCTCAAA TCATAGTCCC CCAACACCAA GAAGGAGTCC CATTCCCAAT
    GATAGGCCAG ATATGAGGCG CACAGATTCT TTACGGAAAC AGCACCACTT GGACCCAAGT
    TCTGCCGTAA GGAAAACCAA AAGGGAAAAA ATGGAAACTA TGTTAAGGAA TGATTCTCTT
    AGCTCAGATC AGTCTGAATC TGTCCGGCCC CCACCACCAA AGCCTCATAA AACAAAAAAG
    GGAGGTAAAA TGCGTCAGGT TTCCTTGAGC AGTTCAGAAG AAGAGTTGGC GTCTACGCCG
    GAGTACACGA GCTGCGATGA TGTGGAGATT GAAAGTGAAA GTGTTAGTGA GAAAGGGGAC
    AGTCAAAGGG GAAAAAGAAA AACTAGTGAG CAGGCAGTTT TGTCGGATTC TAACATCTTG
    TCTGAGAGAC AAAAGAAAAT GGTGTGCTTT GGAGGCCACT CTTTGGAAGA GGATTTGGAA
    TGGTCTGAGC CTCAGATTAA AGACTCTGGG GTAGACACGT GTAGTAGCAC CACCCTTAAC
    GAAGAGCATA GCCATAGCGA GAAGCACCCT GTCACGTGGC AACCTTCCAA AGATGGAGAC
    CGCTTAATTG GTCGCATATT ATTAAACAAG CGGCTCAAAG ATGGGAGCGT CCCGCGAGAT
    TCTGGAGCCA TGCTGGGGCT GAAGGTCGTT GGAGGGAAAA TGACAGAATC AGGTCGACTT
    TGTGCGTTTA TCACCAAAGT GAAGAAGGGA AGCCTGGCAG ATACAGTAGG CCATCTTCGA
    CCAGGTGATG AGGTTTTAGA ATGGAGTGGG CGGATTTTGC AAGGAGCGAC GTTCGAAGAA
    GTTTACAATA TTATACTGGA GTCCAAACCC GAGGCACAAG TAGAATTATT GGTCTCCAGA
    CCCATAGGAG ATATACCCCG GATTCCTGAC AGCACACACG CACAACTGGA ATCCAGTTCT
    AGCTCATTTG AATCCCAGAA AATGGATCGC CCTTCTATTT CAGTTACTTC CCCCATGAGT
    CCTGGAATGT TACGGGATGT CCCCCAGTTT CTTTCTGGAC AACTTTCAAG CCAAAGCCTT
    AATAGAAGAA CAGCGCCTTT TGTTCCTAGG GTTCAGATTA AACTCTGGTA TGACAAGGTT
    GGCCACCAGT TAATTGTCAC GATATTAGGA GCAAAGGATC TGCCATCGCG GGAAGATGGG
    AGGCCGAGAA ATCCTTACGT AAAAATTTAC TTTCTTCCAG ATCGAAGTGA TAAGAACAAA
    AGGAGAACAA AGACTGTGAA GAAAACCGTA GAGCCCAAAT GGAACCAAAC ATTCATCTAT
    TCTCCGGTTC ATCGGAGGGA ATTTCGGGAA CGAATGTTGG AAATAACACT TTGGGATCAA
    GCTCGGGTTC GAGAAGAAGA GAGTGAATTT TTAGGAGAGA TTCTAATTGA ATTAGAAACT
    GCACTATTAG ATGATGAGCC ACATTGGTAC AAGCTACAGA CGCATGATGT GTCTTCATTG
    CCACTTCCAC ACCCATCCCC GTTCATGCAA AGAAGGCAGC TGCACGGCGA GAGCCCAACC
    CGGAGGTTGC AAAATAAAGG CTCATATTCA TATAATTCAG GATCCAAAAG GATAAGCGAC
    AGCGAAGTGT CTGACTACGA CTGTGATGAT GGCGTTGGTG TTGTTTCAGA CTTTCGGCAT
    AATGGGCGTG ACCTGCAGAG TTCAACGTTA TCTGTTCCGG AACAAGTAAT GTCGTCCAAT
    CACTGTTCCC GGACTGGGCC CCCGCATGTA GACAGCATAG GAAGAACGAG GTCATGGTCT
    CCCAGTGTCC CTCCTCCGCA AAGTAGGAAT TTGGAACAGG GACCGAGAGG GACAAGATCT
    GGCCCTGCAC ATTACAATAC CATGAGCAGA GTGGAAAGGC ATCGAGTATT AGATAACCAC
    TACTCCCCAG ACAGAGACAG GAATTGTGAA GCGGCAGATA GACAGCCATA TCAAAGATCC
    AGGTCCACTG AACAACGGCC TGTGCTGGAG CGGACCAGCT CCCGCTCCCG ATCTACTGAG
    CGTCCTGACT CAAACCTCAT GAGGTCGATG CCTTCATTAA TGACGGGAAG ATCTGCCCCT
    CCTTCACCCG CCTTATCGAG GGCACACCCT CCTCGTACCG GCTCTGTTCA GACAAGTCCT
    TCAGGCACCC CGTTAGCAGG GCGGAGGGGG CGCCAGCTCC CGCAACTTCC CCCAAAAGGC
    ACGTTAGAAA GAAAAGTTAC TTGGGAAGAC CAGCAAAGAA AGGTGAATGG TTCAATAACT
    GATGACAAAC ATTTGCCAAG AAAGAAACAG CATTCTGAGC CAGAAGATAT GGACTCAACG
    CGGAGGCGAA ACTCAGAGGA AAAGGCTGCA GAACAAGAGA ATGGGAACCC AGGTGCTATG
    GACGTAGAAG AAAGGACGCG ACAGCTGAAA GTGAAGATCA ACAAATACAA GCAGGTGGGA
    GGATCAGATT CTCGGCTAGA GAAGGATTAT CATCCAAAGT ATCGGACGGG GCGGGATCCG
    CAGAGGGGCT CAGACAATAT GTCCAACAAG TCATCAGACA GCGATGTTAG TGATGTGTCT
    GCCGTATCAC GGACCAGCAG CGCCTCTCGT TTTAGTAGTA CGAGTTATAT GTCTGTGCAG
    TCGGAAAGGC CTCGAGGGAA CATGAAAATA GGTGCATTGG CATCCAAAAT GCAAAGCAGG
    CAAATGGGCG CCTCAGGGAA GAACATGACC AAAAGTACCA GCATCGGAGG GGATATGTAC
    ACCCTGGAGA AGAACGACGG AAGCCAGTCT GACACAGCCG TGGGCACTGT GGGTGCGGGT
    GGAAAGAAGA GACGTTCAAG CATAGGCGCC AAAATGGTGG CCATTGTGGG CTTGTCACGA
    AAAAGCCGAA GTACCTCACA GCTCAGCCAA ACAGAAGTCG GAGGGAAGAA GCTGAAGAGC
    ACCATCCAGA GAAGTACGGA AACAGGCCTG GCCGTGGAAA TGAGAAACTG GATGAGCCGC
    CAAGCAAGCA GGGAGTCCAC AGATGGGAGT ATGAACAGCT ACAGCTCAGA GGGGAATCTG
    ATATTCCCTG GGGTCCGACT GGGTTCCGAC AGCCAGTTCA GTGACTTTTT GGATGGTCTT
    GGTCCTGCCC AGCTGGTTGG TCGCCAGACT TTGGCCACGC CCTCTATGGG AGATATTCAG
    GTTGGAATGG CTGACAAAAA AGGCCAGTTG GAGGTGGAGG TGATACGAGC CCGTGGTCTT
    GTTGTAAAAC CAGGCTCAAA GACACTGCCA GCACCCTATG TTAAGGTGTA TCTCTTAGAA
    AATGGAGTGT GCATAGCCAA AAAGAAAACA AAAGTAGCAA GAAAAACGCT GGAGCCTCTA
    TACCAGCAAG TGTTAGCGTT TGAGGAATCA CCACAAGGGA AGGTCCTACA GATCATTGTT
    TGGGGTGACT ATGGACGCAT GGACCACAAG TCCTTCATGG GGGTGGCTCA GATACTTTTA
    GATGAACTGG ACCTATCCAG CATGGTGATT GGATGGTATA AACTTTTCCC CCCTTCATCT
    TTAGTAGACC CAACGTTGGC TCCACTCACT AGGAGAGCTT CCCAATCATC TCTTGAAAGC
    TCCACGGGAC CTTCTTATGC TCGTTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031759423.1
    CDS420..5429
    Translation

    Target ORF information:

    RefSeq Version XM_031903563.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 2 (rims2), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031903563.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTCTGCTC CACTAGGGCC CAGGGGCGGC CCTCTGCCTC CTGCACAGCC ACAGGCTCCA 
    ATGCCAGAGA TGCCGGACCT CAGCCACCTG ACAGAGGATG AGAGGAAAAT CATAATGGCT
    GTGATGGAGA GGCAGAAGAA AGAAGAGGAG AAGGAGCAGT CAGTGTTAAA GGTCAAAGAA
    GAACAAAAAG CCCAGCCGGC ACAGTGGTTT CCCTTTAGTG GGATCACTGA GCTGGTTAAT
    AACGTTCTTC AGCCACAGCC AAAAGAGCAA AATGAAAAGG AGCCGGAGAT AAAATTGCAC
    CAGCAGTTTG AAATGTACAA GGAGCAAGTG AAGAAGATGG GCGAGGAAGC ACAGCAGCAG
    CAGGAGCAGA AGGGCGAAGC TCCAACCTGT GGAATCTGCC ACAAAACAAA GTTTGCTGAT
    GGCTGTGGCC ACGACTGTTC GTATTGCCAA ACTAAATTCT GCGCGCGGTG TGGAGGGCGA
    GTGTCTCTGC GCTCAAATAA GGAGGACAAA GTGGTAATGT GGGTATGTAA TTTGTGCCGA
    AAACAACAAG AAATCCTCAC CAAATCAGGG GCCTGGTTTT ACAGCAGTGG GTCAAGCACA
    CCCCTAAAGC CCGGCGAAGA GAGCCAGGCA GGGTACCAAA CGGAAGAAGC ACCTCAGGAG
    AAAAAAGCTA AGCTACAAGA GCAGTCCCAA TACCAGGGAC CCTCAGGGGA CTTATTAACA
    CCAGCCTCGG ACAAAAACCG ACCGCAAGGG ATCGTCCGGC AAGATTCCAT CAAAAATGGT
    TCCGAACTGA AGCACCCGAT TCCTGGCGAC CTCGCATCTG ACAGGAAAAG AAGCCCTTCA
    ATACCTAGAG ACCAGAATCG GAGATATGAT ACAAGGGACG ATATAGGTGA ACATTCACAG
    CATGCTACAT CAGACAGCGC CATGCCTAGA TCACCATCAG ACTACTCGGA CAGGCGATTG
    CAACGAGGGT CTCAGCTCTA TGATGAGCCT GAGCACGGTG ACTACAGAAG AAGTAGGCGG
    CGCTCCAATG AGTATTCGGT AGATGAGGAG GATATCCAAG ACAGGGAGGA ATATGAAAGG
    CAGAGAAGAG AGGAGGAGTA CCAGGCAAGG TACCGGAGTG ACCCCAACTT GGCCAGGTAC
    CCTGTTAAGC CACAACCGTA TGAAGAGCAA ATGCGCATCC ATGCAGAGGT TTCAAGGGCT
    CGACAACGAA GGCACAGCGA TGTGTCTTTA GCTCAGACAG AATTTGAGGA GTCTCGGATG
    TCAACGCTGA GGATGGAGAG ACCTTCAAGG CAAAGATCTG CGTCAGAGCG TAGAGCAGCG
    ATGGAAAGCC AGCGTTCATA TTCAATAGAA AGAACTAGAG ATGTGCAAGG GCCAGGTCCT
    GGTCGGCAAA GGACCTCAAA TCATAGTCCC CCAACACCAA GAAGGAGTCC CATTCCCAAT
    GATAGGCCAG ATATGAGGCG CACAGATTCT TTACGGAAAC AGCACCACTT GGACCCAAGT
    TCTGCCGTAA GGAAAACCAA AAGGGAAAAA ATGGAAACTA TGTTAAGGAA TGATTCTCTT
    AGCTCAGATC AGTCTGAATC TGTCCGGCCC CCACCACCAA AGCCTCATAA AACAAAAAAG
    GGAGGTAAAA TGCGTCAGGT TTCCTTGAGC AGTTCAGAAG AAGAGTTGGC GTCTACGCCG
    GAGTACACGA GCTGCGATGA TGTGGAGATT GAAAGTGAAA GTGTTAGTGA GAAAGGGGAC
    AGTCAAAGGG GAAAAAGAAA AACTAGTGAG CAGGCAGTTT TGTCGGATTC TAACATCTTG
    TCTGAGAGAC AAAAGAAAAT GGTGTGCTTT GGAGGCCACT CTTTGGAAGA GGATTTGGAA
    TGGTCTGAGC CTCAGATTAA AGACTCTGGG GTAGACACGT GTAGTAGCAC CACCCTTAAC
    GAAGAGCATA GCCATAGCGA GAAGCACCCT GTCACGTGGC AACCTTCCAA AGATGGAGAC
    CGCTTAATTG GTCGCATATT ATTAAACAAG CGGCTCAAAG ATGGGAGCGT CCCGCGAGAT
    TCTGGAGCCA TGCTGGGGCT GAAGGTCGTT GGAGGGAAAA TGACAGAATC AGGTCGACTT
    TGTGCGTTTA TCACCAAAGT GAAGAAGGGA AGCCTGGCAG ATACAGTAGG CCATCTTCGA
    CCAGGTGATG AGGTTTTAGA ATGGAGTGGG CGGATTTTGC AAGGAGCGAC GTTCGAAGAA
    GTTTACAATA TTATACTGGA GTCCAAACCC GAGGCACAAG TAGAATTATT GGTCTCCAGA
    CCCATAGGAG ATATACCCCG GATTCCTGAC AGCACACACG CACAACTGGA ATCCAGTTCT
    AGCTCATTTG AATCCCAGAA AATGGATCGC CCTTCTATTT CAGTTACTTC CCCCATGAGT
    CCTGGAATGT TACGGGATGT CCCCCAGTTT CTTTCTGGAC AACTTTCAAG CCAAAGCCTT
    AATAGAAGAA CAGCGCCTTT TGTTCCTAGG GTTCAGATTA AACTCTGGTA TGACAAGGTT
    GGCCACCAGT TAATTGTCAC GATATTAGGA GCAAAGGATC TGCCATCGCG GGAAGATGGG
    AGGCCGAGAA ATCCTTACGT AAAAATTTAC TTTCTTCCAG ATCGAAGTGA TAAGAACAAA
    AGGAGAACAA AGACTGTGAA GAAAACCGTA GAGCCCAAAT GGAACCAAAC ATTCATCTAT
    TCTCCGGTTC ATCGGAGGGA ATTTCGGGAA CGAATGTTGG AAATAACACT TTGGGATCAA
    GCTCGGGTTC GAGAAGAAGA GAGTGAATTT TTAGGAGAGA TTCTAATTGA ATTAGAAACT
    GCACTATTAG ATGATGAGCC ACATTGGTAC AAGCTACAGA CGCATGATGT GTCTTCATTG
    CCACTTCCAC ACCCATCCCC GTTCATGCAA AGAAGGCAGC TGCACGGCGA GAGCCCAACC
    CGGAGGTTGC AAAATAAAGG CTCATATTCA TATAATTCAG GATCCAAAAG GATAAGCGAC
    AGCGAAGTGT CTGACTACGA CTGTGATGAT GGCGTTGGTG TTGTTTCAGA CTTTCGGCAT
    AATGGGCGTG ACCTGCAGAG TTCAACGTTA TCTGTTCCGG AACAAGTAAT GTCGTCCAAT
    CACTGTTCCC GGACTGGGCC CCCGCATGTA GACAGCATAG GAAGAACGAG GTCATGGTCT
    CCCAGTGTCC CTCCTCCGCA AAGTAGGAAT TTGGAACAGG GACCGAGAGG GACAAGATCT
    GGCCCTGCAC ATTACAATAC CATGAGCAGA GTGGAAAGGC ATCGAGTATT AGATAACCAC
    TACTCCCCAG ACAGAGACAG GAATTGTGAA GCGGCAGATA GACAGCCATA TCAAAGATCC
    AGGTCCACTG AACAACGGCC TGTGCTGGAG CGGACCAGCT CCCGCTCCCG ATCTACTGAG
    CGTCCTGACT CAAACCTCAT GAGGTCGATG CCTTCATTAA TGACGGGAAG ATCTGCCCCT
    CCTTCACCCG CCTTATCGAG GGCACACCCT CCTCGTACCG GCTCTGTTCA GACAAGTCCT
    TCAGGCACCC CGTTAGCAGG GCGGAGGGGG CGCCAGCTCC CGCAACTTCC CCCAAAAGGC
    ACGTTAGAAA GAAAAGTTAC TTGGGAAGAC CAGCAAAGAA AGGTGAATGG TTCAATAACT
    GATGACAAAC ATTTGCCAAG AAAGAAACAG CATTCTGAGC CAGAAGATAT GGACTCAACG
    CGGAGGCGAA ACTCAGAGGA AAAGGCTGCA GAACAAGAGA ATGGGAACCC AGGTGCTATG
    GACGTAGAAG AAAGGACGCG ACAGCTGAAA GTGAAGATCA ACAAATACAA GCAGGTGGGA
    GGATCAGATT CTCGGCTAGA GAAGGATTAT CATCCAAAGT ATCGGACGGG GCGGGATCCG
    CAGAGGGGCT CAGACAATAT GTCCAACAAG TCATCAGACA GCGATGTTAG TGATGTGTCT
    GCCGTATCAC GGACCAGCAG CGCCTCTCGT TTTAGTAGTA CGAGTTATAT GTCTGTGCAG
    TCGGAAAGGC CTCGAGGGAA CATGAAAATA GGTGCATTGG CATCCAAAAT GCAAAGCAGG
    CAAATGGGCG CCTCAGGGAA GAACATGACC AAAAGTACCA GCATCGGAGG GGATATGTAC
    ACCCTGGAGA AGAACGACGG AAGCCAGTCT GACACAGCCG TGGGCACTGT GGGTGCGGGT
    GGAAAGAAGA GACGTTCAAG CATAGGCGCC AAAATGGTGG CCATTGTGGG CTTGTCACGA
    AAAAGCCGAA GTACCTCACA GCTCAGCCAA ACAGAAGTCG GAGGGAAGAA GCTGAAGAGC
    ACCATCCAGA GAAGTACGGA AACAGGCCTG GCCGTGGAAA TGAGAAACTG GATGAGCCGC
    CAAGCAAGCA GGGAGTCCAC AGATGGGAGT ATGAACAGCT ACAGCTCAGA GGGGAATCTG
    ATATTCCCTG GGGTCCGACT GGGTTCCGAC AGCCAGTTCA GTGACTTTTT GGATGGTCTT
    GGTCCTGCCC AGCTGGTTGG TCGCCAGACT TTGGCCACGC CCTCTATGGG AGATATTCAG
    GTTGGAATGG CTGACAAAAA AGGCCAGTTG GAGGTGGAGG TGATACGAGC CCGTGGTCTT
    GTTGTAAAAC CAGGCTCAAA GACACTGCCA GCACCCTATG TTAAGGTGTA TCTCTTAGAA
    AATGGAGTGT GCATAGCCAA AAAGAAAACA AAAGTAGCAA GAAAAACGCT GGAGCCTCTA
    TACCAGCAAG TGTTAGCGTT TGAGGAATCA CCACAAGGGA AGGTCCTACA GATCATTGTT
    TGGGGTGACT ATGGACGCAT GGACCACAAG TCCTTCATGG GGGTGGCTCA GATACTTTTA
    GATGAACTGG ACCTATCCAG CATGGTGATT GGATGGTATA AACTTTTCCC CCCTTCATCT
    TTAGTAGACC CAACGTTGGC TCCACTCACT AGGAGAGCTT CCCAATCATC TCTTGAAAGC
    TCCACGGGAC CTTCTTATGC TCGTTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa59909
    Clone ID Related Accession (Same CDS sequence) XM_031903568.1
    Accession Version XM_031903568.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4851bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 2 isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTCTGCTC CACTAGGGCC CAGGGGCGGC CCTCTGCCTC CTGCACAGCC ACAGGCTCCA 
    ATGCCAGAGA TGCCGGACCT CAGCCACCTG ACAGAGGATG AGAGGAAAAT CATAATGGCT
    GTGATGGAGA GGCAGAAGAA AGAAGAGGAG AAGGAGCAGT CAGTGTTAAA GGTCAAAGAA
    GAACAAAAAG CCCAGCCGGC ACAGTGGTTT CCCTTTAGTG GGATCACTGA GCTGGTTAAT
    AACGTTCTTC AGCCACAGCC AAAAGAGCAA AATGAAAAGG AGCCGGAGAT AAAATTGCAC
    CAGCAGTTTG AAATGTACAA GGAGCAAGTG AAGAAGATGG GCGAGGAAGC ACAGCAGCAG
    CAGGAGCAGA AGGGCGAAGC TCCAACCTGT GGAATCTGCC ACAAAACAAA GTTTGCTGAT
    GGCTGTGGCC ACGACTGTTC GTATTGCCAA ACTAAATTCT GCGCGCGGTG TGGAGGGCGA
    GTGTCTCTGC GCTCAAATAA GGAGGACAAA GTGGTAATGT GGGTATGTAA TTTGTGCCGA
    AAACAACAAG AAATCCTCAC CAAATCAGGG GCCTGGTTTT ACAGCAGTGG GTCAAGCACA
    CCCCTAAAGC CCGGCGAAGA GAGCCAGGCA GGGTACCAAA CGGAAGAAGC ACCTCAGGAG
    AAAAAAGCTA AGCTACAAGA GCAGTCCCAA TACCAGGGAC CCTCAGGGGA CTTATTAACA
    CCAGCCTCGG ACAAAAACCG ACCGCAAGGG ATCGTCCGGC AAGATTCCAT CAAAAATGGT
    TCCGAACTGA AGCACCCGAT TCCTGGCGAC CTCGCATCTG ACAGGAAAAG AAGCCCTTCA
    ATACCTAGAG ACCAGAATCG GAGATATGAT ACAAGGGACG ATATAGGTGA ACATTCACAG
    CATGCTACAT CAGACAGCGC CATGCCTAGA TCACCATCAG ACTACTCGGA CAGGCGATTG
    CAACGAGGGT CTCAGCTCTA TGATGAGCCT GAGCACGGTG ACTACAGAAG AAGTAGGCGG
    CGCTCCAATG AGTATTCGGT AGATGAGGAG GATATCCAAG ACAGGGAGGA ATATGAAAGG
    CAGAGAAGAG AGGAGGAGTA CCAGGCAAGG TACCGGAGTG ACCCCAACTT GGCCAGGTAC
    CCTGTTAAGC CACAACCGTA TGAAGAGCAA ATGCGCATCC ATGCAGAGGT TTCAAGGGCT
    CGACAACGAA GGCACAGCGA TGTGTCTTTA GCTCAGACAG AATTTGAGGA GTCTCGGATG
    TCAACGCTGA GGATGGAGAG ACCTTCAAGG CAAAGATCTG CGTCAGAGCG TAGAGCAGCG
    ATGGAAAGCC AGCGTTCATA TTCAATAGAA AGAACTAGAG ATGTGCAAGG GCCAGGTCCT
    GGTCGGCAAA GGACCTCAAA TCATAGTCCC CCAACACCAA GAAGGAGTCC CATTCCCAAT
    GATAGGCCAG ATATGAGGCG CACAGATTCT TTACGGAAAC AGCACCACTT GGACCCAAGT
    TCTGCCGTAA GGAAAACCAA AAGGGAAAAA ATGGAAACTA TGTTAAGGAA TGATTCTCTT
    AGCTCAGATC AGTCTGAATC TGTCCGGCCC CCACCACCAA AGCCTCATAA AACAAAAAAG
    GGAGGTAAAA TGCGTCAGGT TTCCTTGAGC AGTTCAGAAG AAGAGTTGGC GTCTACGCCG
    GAGTACACGA GCTGCGATGA TGTGGAGATT GAAAGTGAAA GTGTTAGTGA GAAAGGGGAC
    AGTCAAAGGG GAAAAAGAAA AACTAGTGAG CAGGCAGTTT TGTCGGATTC TAACATCTTG
    TCTGAGAGAC AAAAGAAAAT GGTGTGCTTT GGAGGCCACT CTTTGGAAGA GGATTTGGAA
    TGGTCTGAGC CTCAGATTAA AGACTCTGGG GTAGACACGT GTAGTAGCAC CACCCTTAAC
    GAAGAGCATA GCCATAGCGA GAAGCACCCT GTCACGTGGC AACCTTCCAA AGATGGAGAC
    CGCTTAATTG GTCGCATATT ATTAAACAAG CGGCTCAAAG ATGGGAGCGT CCCGCGAGAT
    TCTGGAGCCA TGCTGGGGCT GAAGGTCGTT GGAGGGAAAA TGACAGAATC AGGTCGACTT
    TGTGCGTTTA TCACCAAAGT GAAGAAGGGA AGCCTGGCAG ATACAGTAGG CCATCTTCGA
    CCAGGTGATG AGGTTTTAGA ATGGAGTGGG CGGATTTTGC AAGGAGCGAC GTTCGAAGAA
    GTTTACAATA TTATACTGGA GTCCAAACCC GAGGCACAAG TAGAATTATT GGTCTCCAGA
    CCCATAGGAG ATATACCCCG GATTCCTGAC AGCACACACG CACAACTGGA ATCCAGTTCT
    AGCTCATTTG AATCCCAGAA AATGGATCGC CCTTCTATTT CAGTTACTTC CCCCATGAGT
    CCTGGAATGT TACGGGATGT CCCCCAGTTT CTTTCTGGAC AACTTTCAAG CCAAAGCCTT
    AATAGAAGAA CAGCGCCTTT TGTTCCTAGG GTTCAGATTA AACTCTGGTA TGACAAGGTT
    GGCCACCAGT TAATTGTCAC GATATTAGGA GCAAAGGATC TGCCATCGCG GGAAGATGGG
    AGGCCGAGAA ATCCTTACGT AAAAATTTAC TTTCTTCCAG ATCGAAGTGA TAAGAACAAA
    AGGAGAACAA AGACTGTGAA GAAAACCGTA GAGCCCAAAT GGAACCAAAC ATTCATCTAT
    TCTCCGGTTC ATCGGAGGGA ATTTCGGGAA CGAATGTTGG AAATAACACT TTGGGATCAA
    GCTCGGGTTC GAGAAGAAGA GAGTGAATTT TTAGGAGAGA TTCTAATTGA ATTAGAAACT
    GCACTATTAG ATGATGAGCC ACATTGGTAC AAGCTACAGA CGCATGATGT GTCTTCATTG
    CCACTTCCAC ACCCATCCCC GTTCATGCAA AGAAGGCAGC TGCACGGCGA GAGCCCAACC
    CGGAGGTTGC AAAATAAAGG CTCATATTCA TATAATTCAG GATCCAAAAG GATAAGCGAC
    AGCGAAGTGT CTGACTACGA CTGTGATGAT GGCGTTGGTG TTGTTTCAGA CTTTCGGCAT
    AATGGGCGTG ACCTGCAGAG TTCAACGTTA TCTGTTCCGG AACAAGTAAT GTCGTCCAAT
    CACTGTTCCC GGACTGGGCC CCCGCATGTA GACAGCATAG GAAGAACGAG GTCATGGTCT
    CCCAGTGTCC CTCCTCCGCA AAGTAGGAAT TTGGAACAGG GACCGAGAGG GACAAGATCT
    GGCCCTGCAC ATTACAATAC CATGAGCAGA GTGGAAAGGC ATCGAGTATT AGATAACCAC
    TACTCCCCAG ACAGAGACAG GAATTGTGAA GCGGCAGATA GACAGCCATA TCAAAGATCC
    AGGTCCACTG AACAACGGCC TGTGCTGGAG CGGACCAGCT CCCGCTCCCG ATCTACTGAG
    CGTCCTGACT CAAACCTCAT GAGGTCGATG CCTTCATTAA TGACGGGAAG ATCTGCCCCT
    CCTTCACCCG CCTTATCGAG GGCACACCCT CCTCGTACCG GCTCTGTTCA GACAAGTCCT
    TCAGGCACCC CGTTAGCAGG GCGGAGGGGG CGCCAGCTCC CGCAACTTCC CCCAAAAGGC
    ACGTTAGAAA GAAGTGCTAT GGACGTAGAA GAAAGGACGC GACAGCTGAA AGTGAAGATC
    AACAAATACA AGCAGGTGGG AGGATCAGAT TCTCGGCTAG AGAAGGATTA TCATCCAAAG
    TATCGGACGG GGCGGGATCC GCAGAGGGGC TCAGACAATA TGTCCAACAA GTCATCAGAC
    AGCGATGTTA GTGATGTGTC TGCCGTATCA CGGACCAGCA GCGCCTCTCG TTTTAGTAGT
    ACGAGTTATA TGTCTGTGCA GTCGGAAAGG CCTCGAGGGA ACATGAAAAT AGGTGCATTG
    GCATCCAAAA TGCAAAGCAG GCAAATGGGC GCCTCAGGGA AGAACATGAC CAAAAGTACC
    AGCATCGGAG GGGATATGTA CACCCTGGAG AAGAACGACG GAAGCCAGTC TGACACAGCC
    GTGGGCACTG TGGGTGCGGG TGGAAAGAAG AGACGTTCAA GCATAGGCGC CAAAATGGTG
    GCCATTGTGG GCTTGTCACG AAAAAGCCGA AGTACCTCAC AGCTCAGCCA AACAGAAGTC
    GGAGGGAAGA AGCTGAAGAG CACCATCCAG AGAAGTACGG AAACAGGCCT GGCCGTGGAA
    ATGAGAAACT GGATGAGCCG CCAAGCAAGC AGGGAGTCCA CAGATGGGAG TATGAACAGC
    TACAGCTCAG AGGGGAATCT GATATTCCCT GGGGTCCGAC TGGGTTCCGA CAGCCAGTTC
    AGTGACTTTT TGGATGGTCT TGGTCCTGCC CAGCTGGTTG GTCGCCAGAC TTTGGCCACG
    CCCTCTATGG GAGATATTCA GGTTGGAATG GCTGACAAAA AAGGCCAGTT GGAGGTGGAG
    GTGATACGAG CCCGTGGTCT TGTTGTAAAA CCAGGCTCAA AGACACTGCC AGCACCCTAT
    GTTAAGGTGT ATCTCTTAGA AAATGGAGTG TGCATAGCCA AAAAGAAAAC AAAAGTAGCA
    AGAAAAACGC TGGAGCCTCT ATACCAGCAA GTGTTAGCGT TTGAGGAATC ACCACAAGGG
    AAGGTCCTAC AGATCATTGT TTGGGGTGAC TATGGACGCA TGGACCACAA GTCCTTCATG
    GGGGTGGCTC AGATACTTTT AGATGAACTG GACCTATCCA GCATGGTGAT TGGATGGTAT
    AAACTTTTCC CCCCTTCATC TTTAGTAGAC CCAACGTTGG CTCCACTCAC TAGGAGAGCT
    TCCCAATCAT CTCTTGAAAG CTCCACGGGA CCTTCTTATG CTCGTTCATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031759428.1
    CDS419..5269
    Translation

    Target ORF information:

    RefSeq Version XM_031903568.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 2 (rims2), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031903568.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTCTGCTC CACTAGGGCC CAGGGGCGGC CCTCTGCCTC CTGCACAGCC ACAGGCTCCA 
    ATGCCAGAGA TGCCGGACCT CAGCCACCTG ACAGAGGATG AGAGGAAAAT CATAATGGCT
    GTGATGGAGA GGCAGAAGAA AGAAGAGGAG AAGGAGCAGT CAGTGTTAAA GGTCAAAGAA
    GAACAAAAAG CCCAGCCGGC ACAGTGGTTT CCCTTTAGTG GGATCACTGA GCTGGTTAAT
    AACGTTCTTC AGCCACAGCC AAAAGAGCAA AATGAAAAGG AGCCGGAGAT AAAATTGCAC
    CAGCAGTTTG AAATGTACAA GGAGCAAGTG AAGAAGATGG GCGAGGAAGC ACAGCAGCAG
    CAGGAGCAGA AGGGCGAAGC TCCAACCTGT GGAATCTGCC ACAAAACAAA GTTTGCTGAT
    GGCTGTGGCC ACGACTGTTC GTATTGCCAA ACTAAATTCT GCGCGCGGTG TGGAGGGCGA
    GTGTCTCTGC GCTCAAATAA GGAGGACAAA GTGGTAATGT GGGTATGTAA TTTGTGCCGA
    AAACAACAAG AAATCCTCAC CAAATCAGGG GCCTGGTTTT ACAGCAGTGG GTCAAGCACA
    CCCCTAAAGC CCGGCGAAGA GAGCCAGGCA GGGTACCAAA CGGAAGAAGC ACCTCAGGAG
    AAAAAAGCTA AGCTACAAGA GCAGTCCCAA TACCAGGGAC CCTCAGGGGA CTTATTAACA
    CCAGCCTCGG ACAAAAACCG ACCGCAAGGG ATCGTCCGGC AAGATTCCAT CAAAAATGGT
    TCCGAACTGA AGCACCCGAT TCCTGGCGAC CTCGCATCTG ACAGGAAAAG AAGCCCTTCA
    ATACCTAGAG ACCAGAATCG GAGATATGAT ACAAGGGACG ATATAGGTGA ACATTCACAG
    CATGCTACAT CAGACAGCGC CATGCCTAGA TCACCATCAG ACTACTCGGA CAGGCGATTG
    CAACGAGGGT CTCAGCTCTA TGATGAGCCT GAGCACGGTG ACTACAGAAG AAGTAGGCGG
    CGCTCCAATG AGTATTCGGT AGATGAGGAG GATATCCAAG ACAGGGAGGA ATATGAAAGG
    CAGAGAAGAG AGGAGGAGTA CCAGGCAAGG TACCGGAGTG ACCCCAACTT GGCCAGGTAC
    CCTGTTAAGC CACAACCGTA TGAAGAGCAA ATGCGCATCC ATGCAGAGGT TTCAAGGGCT
    CGACAACGAA GGCACAGCGA TGTGTCTTTA GCTCAGACAG AATTTGAGGA GTCTCGGATG
    TCAACGCTGA GGATGGAGAG ACCTTCAAGG CAAAGATCTG CGTCAGAGCG TAGAGCAGCG
    ATGGAAAGCC AGCGTTCATA TTCAATAGAA AGAACTAGAG ATGTGCAAGG GCCAGGTCCT
    GGTCGGCAAA GGACCTCAAA TCATAGTCCC CCAACACCAA GAAGGAGTCC CATTCCCAAT
    GATAGGCCAG ATATGAGGCG CACAGATTCT TTACGGAAAC AGCACCACTT GGACCCAAGT
    TCTGCCGTAA GGAAAACCAA AAGGGAAAAA ATGGAAACTA TGTTAAGGAA TGATTCTCTT
    AGCTCAGATC AGTCTGAATC TGTCCGGCCC CCACCACCAA AGCCTCATAA AACAAAAAAG
    GGAGGTAAAA TGCGTCAGGT TTCCTTGAGC AGTTCAGAAG AAGAGTTGGC GTCTACGCCG
    GAGTACACGA GCTGCGATGA TGTGGAGATT GAAAGTGAAA GTGTTAGTGA GAAAGGGGAC
    AGTCAAAGGG GAAAAAGAAA AACTAGTGAG CAGGCAGTTT TGTCGGATTC TAACATCTTG
    TCTGAGAGAC AAAAGAAAAT GGTGTGCTTT GGAGGCCACT CTTTGGAAGA GGATTTGGAA
    TGGTCTGAGC CTCAGATTAA AGACTCTGGG GTAGACACGT GTAGTAGCAC CACCCTTAAC
    GAAGAGCATA GCCATAGCGA GAAGCACCCT GTCACGTGGC AACCTTCCAA AGATGGAGAC
    CGCTTAATTG GTCGCATATT ATTAAACAAG CGGCTCAAAG ATGGGAGCGT CCCGCGAGAT
    TCTGGAGCCA TGCTGGGGCT GAAGGTCGTT GGAGGGAAAA TGACAGAATC AGGTCGACTT
    TGTGCGTTTA TCACCAAAGT GAAGAAGGGA AGCCTGGCAG ATACAGTAGG CCATCTTCGA
    CCAGGTGATG AGGTTTTAGA ATGGAGTGGG CGGATTTTGC AAGGAGCGAC GTTCGAAGAA
    GTTTACAATA TTATACTGGA GTCCAAACCC GAGGCACAAG TAGAATTATT GGTCTCCAGA
    CCCATAGGAG ATATACCCCG GATTCCTGAC AGCACACACG CACAACTGGA ATCCAGTTCT
    AGCTCATTTG AATCCCAGAA AATGGATCGC CCTTCTATTT CAGTTACTTC CCCCATGAGT
    CCTGGAATGT TACGGGATGT CCCCCAGTTT CTTTCTGGAC AACTTTCAAG CCAAAGCCTT
    AATAGAAGAA CAGCGCCTTT TGTTCCTAGG GTTCAGATTA AACTCTGGTA TGACAAGGTT
    GGCCACCAGT TAATTGTCAC GATATTAGGA GCAAAGGATC TGCCATCGCG GGAAGATGGG
    AGGCCGAGAA ATCCTTACGT AAAAATTTAC TTTCTTCCAG ATCGAAGTGA TAAGAACAAA
    AGGAGAACAA AGACTGTGAA GAAAACCGTA GAGCCCAAAT GGAACCAAAC ATTCATCTAT
    TCTCCGGTTC ATCGGAGGGA ATTTCGGGAA CGAATGTTGG AAATAACACT TTGGGATCAA
    GCTCGGGTTC GAGAAGAAGA GAGTGAATTT TTAGGAGAGA TTCTAATTGA ATTAGAAACT
    GCACTATTAG ATGATGAGCC ACATTGGTAC AAGCTACAGA CGCATGATGT GTCTTCATTG
    CCACTTCCAC ACCCATCCCC GTTCATGCAA AGAAGGCAGC TGCACGGCGA GAGCCCAACC
    CGGAGGTTGC AAAATAAAGG CTCATATTCA TATAATTCAG GATCCAAAAG GATAAGCGAC
    AGCGAAGTGT CTGACTACGA CTGTGATGAT GGCGTTGGTG TTGTTTCAGA CTTTCGGCAT
    AATGGGCGTG ACCTGCAGAG TTCAACGTTA TCTGTTCCGG AACAAGTAAT GTCGTCCAAT
    CACTGTTCCC GGACTGGGCC CCCGCATGTA GACAGCATAG GAAGAACGAG GTCATGGTCT
    CCCAGTGTCC CTCCTCCGCA AAGTAGGAAT TTGGAACAGG GACCGAGAGG GACAAGATCT
    GGCCCTGCAC ATTACAATAC CATGAGCAGA GTGGAAAGGC ATCGAGTATT AGATAACCAC
    TACTCCCCAG ACAGAGACAG GAATTGTGAA GCGGCAGATA GACAGCCATA TCAAAGATCC
    AGGTCCACTG AACAACGGCC TGTGCTGGAG CGGACCAGCT CCCGCTCCCG ATCTACTGAG
    CGTCCTGACT CAAACCTCAT GAGGTCGATG CCTTCATTAA TGACGGGAAG ATCTGCCCCT
    CCTTCACCCG CCTTATCGAG GGCACACCCT CCTCGTACCG GCTCTGTTCA GACAAGTCCT
    TCAGGCACCC CGTTAGCAGG GCGGAGGGGG CGCCAGCTCC CGCAACTTCC CCCAAAAGGC
    ACGTTAGAAA GAAGTGCTAT GGACGTAGAA GAAAGGACGC GACAGCTGAA AGTGAAGATC
    AACAAATACA AGCAGGTGGG AGGATCAGAT TCTCGGCTAG AGAAGGATTA TCATCCAAAG
    TATCGGACGG GGCGGGATCC GCAGAGGGGC TCAGACAATA TGTCCAACAA GTCATCAGAC
    AGCGATGTTA GTGATGTGTC TGCCGTATCA CGGACCAGCA GCGCCTCTCG TTTTAGTAGT
    ACGAGTTATA TGTCTGTGCA GTCGGAAAGG CCTCGAGGGA ACATGAAAAT AGGTGCATTG
    GCATCCAAAA TGCAAAGCAG GCAAATGGGC GCCTCAGGGA AGAACATGAC CAAAAGTACC
    AGCATCGGAG GGGATATGTA CACCCTGGAG AAGAACGACG GAAGCCAGTC TGACACAGCC
    GTGGGCACTG TGGGTGCGGG TGGAAAGAAG AGACGTTCAA GCATAGGCGC CAAAATGGTG
    GCCATTGTGG GCTTGTCACG AAAAAGCCGA AGTACCTCAC AGCTCAGCCA AACAGAAGTC
    GGAGGGAAGA AGCTGAAGAG CACCATCCAG AGAAGTACGG AAACAGGCCT GGCCGTGGAA
    ATGAGAAACT GGATGAGCCG CCAAGCAAGC AGGGAGTCCA CAGATGGGAG TATGAACAGC
    TACAGCTCAG AGGGGAATCT GATATTCCCT GGGGTCCGAC TGGGTTCCGA CAGCCAGTTC
    AGTGACTTTT TGGATGGTCT TGGTCCTGCC CAGCTGGTTG GTCGCCAGAC TTTGGCCACG
    CCCTCTATGG GAGATATTCA GGTTGGAATG GCTGACAAAA AAGGCCAGTT GGAGGTGGAG
    GTGATACGAG CCCGTGGTCT TGTTGTAAAA CCAGGCTCAA AGACACTGCC AGCACCCTAT
    GTTAAGGTGT ATCTCTTAGA AAATGGAGTG TGCATAGCCA AAAAGAAAAC AAAAGTAGCA
    AGAAAAACGC TGGAGCCTCT ATACCAGCAA GTGTTAGCGT TTGAGGAATC ACCACAAGGG
    AAGGTCCTAC AGATCATTGT TTGGGGTGAC TATGGACGCA TGGACCACAA GTCCTTCATG
    GGGGTGGCTC AGATACTTTT AGATGAACTG GACCTATCCA GCATGGTGAT TGGATGGTAT
    AAACTTTTCC CCCCTTCATC TTTAGTAGAC CCAACGTTGG CTCCACTCAC TAGGAGAGCT
    TCCCAATCAT CTCTTGAAAG CTCCACGGGA CCTTCTTATG CTCGTTCATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa59910
    Clone ID Related Accession (Same CDS sequence) XM_031903569.1
    Accession Version XM_031903569.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4827bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 2 isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTCTGCTC CACTAGGGCC CAGGGGCGGC CCTCTGCCTC CTGCACAGCC ACAGGCTCCA 
    ATGCCAGAGA TGCCGGACCT CAGCCACCTG ACAGAGGATG AGAGGAAAAT CATAATGGCT
    GTGATGGAGA GGCAGAAGAA AGAAGAGGAG AAGGAGCAGT CAGTGTTAAA AAAATTGCAC
    CAGCAGTTTG AAATGTACAA GGAGCAAGTG AAGAAGATGG GCGAGGAAGC ACAGCAGCAG
    CAGGAGCAGA AGGGCGAAGC TCCAACCTGT GGAATCTGCC ACAAAACAAA GTTTGCTGAT
    GGCTGTGGCC ACGACTGTTC GTATTGCCAA ACTAAATTCT GCGCGCGGTG TGGAGGGCGA
    GTGTCTCTGC GCTCAAATAA GGAGGACAAA GTGGTAATGT GGGTATGTAA TTTGTGCCGA
    AAACAACAAG AAATCCTCAC CAAATCAGGG GCCTGGTTTT ACAGCAGTGG GTCAAGCACA
    CCCCTAAAGC CCGGCGAAGA GAGCCAGGCA GGGTACCAAA CGGAAGAAGC ACCTCAGGAG
    AAAAAAGCTA AGCTACAAGA GCAGTCCCAA TACCAGGGAC CCTCAGGGGA CTTATTAACA
    CCAGCCTCGG ACAAAAACCG ACCGCAAGGG ATCGTCCGGC AAGATTCCAT CAAAAATGGT
    TCCGAACTGA AGCACCCGAT TCCTGGCGAC CTCGCATCTG ACAGGAAAAG AAGCCCTTCA
    ATACCTAGAG ACCAGAATCG GAGATATGAT ACAAGGGACG ATATAGGTGA ACATTCACAG
    CATGCTACAT CAGACAGCGC CATGCCTAGA TCACCATCAG ACTACTCGGA CAGGCGATTG
    CAACGAGGGT CTCAGCTCTA TGATGAGCCT GAGCACGGTG ACTACAGAAG AAGTAGGCGG
    CGCTCCAATG AGTATTCGGT AGATGAGGAG GATATCCAAG ACAGGGAGGA ATATGAAAGG
    CAGAGAAGAG AGGAGGAGTA CCAGGCAAGG TACCGGAGTG ACCCCAACTT GGCCAGGTAC
    CCTGTTAAGC CACAACCGTA TGAAGAGCAA ATGCGCATCC ATGCAGAGGT TTCAAGGGCT
    CGACAACGAA GGCACAGCGA TGTGTCTTTA GCTCAGACAG AATTTGAGGA GTCTCGGATG
    TCAACGCTGA GGATGGAGAG ACCTTCAAGG CAAAGATCTG CGTCAGAGCG TAGAGCAGCG
    ATGGAAAGCC AGCGTTCATA TTCAATAGAA AGAACTAGAG ATGTGCAAGG GCCAGGTCCT
    GGTCGGCAAA GGACCTCAAA TCATAGTCCC CCAACACCAA GAAGGAGTCC CATTCCCAAT
    GATAGGCCAG ATATGAGGCG CACAGATTCT TTACGGAAAC AGCACCACTT GGACCCAAGT
    TCTGCCGTAA GGAAAACCAA AAGGGAAAAA ATGGAAACTA TGTTAAGGAA TGATTCTCTT
    AGCTCAGATC AGTCTGAATC TGTCCGGCCC CCACCACCAA AGCCTCATAA AACAAAAAAG
    GGAGGTAAAA TGCGTCAGGT TTCCTTGAGC AGTTCAGAAG AAGAGTTGGC GTCTACGCCG
    GAGTACACGA GCTGCGATGA TGTGGAGATT GAAAGTGAAA GTGTTAGTGA GAAAGGTGAC
    ATGGACTACA ACTGGGTGGA GCATTCGTCT TGGCACAGCA GTGAGGCCTC CCCAATGTCT
    TTGCACCCTG TCACGTGGCA ACCTTCCAAA GATGGAGACC GCTTAATTGG TCGCATATTA
    TTAAACAAGC GGCTCAAAGA TGGGAGCGTC CCGCGAGATT CTGGAGCCAT GCTGGGGCTG
    AAGGTCGTTG GAGGGAAAAT GACAGAATCA GGTCGACTTT GTGCGTTTAT CACCAAAGTG
    AAGAAGGGAA GCCTGGCAGA TACAGTAGGC CATCTTCGAC CAGGTGATGA GGTTTTAGAA
    TGGAGTGGGC GGATTTTGCA AGGAGCGACG TTCGAAGAAG TTTACAATAT TATACTGGAG
    TCCAAACCCG AGGCACAAGT AGAATTATTG GTCTCCAGAC CCATAGGAGA TATACCCCGG
    ATTCCTGACA GCACACACGC ACAACTGGAA TCCAGTTCTA GCTCATTTGA ATCCCAGAAA
    ATGGATCGCC CTTCTATTTC AGTTACTTCC CCCATGAGTC CTGGAATGTT ACGGGATGTC
    CCCCAGTTTC TTTCTGGACA ACTTTCAATT AAACTCTGGT ATGACAAGGT TGGCCACCAG
    TTAATTGTCA CGATATTAGG AGCAAAGGAT CTGCCATCGC GGGAAGATGG GAGGCCGAGA
    AATCCTTACG TAAAAATTTA CTTTCTTCCA GATCGAAGTG ATAAGAACAA AAGGAGAACA
    AAGACTGTGA AGAAAACCGT AGAGCCCAAA TGGAACCAAA CATTCATCTA TTCTCCGGTT
    CATCGGAGGG AATTTCGGGA ACGAATGTTG GAAATAACAC TTTGGGATCA AGCTCGGGTT
    CGAGAAGAAG AGAGTGAATT TTTAGGAGAG ATTCTAATTG AATTAGAAAC TGCACTATTA
    GATGATGAGC CACATTGGTA CAAGCTACAG ACGCATGATG TGTCTTCATT GCCACTTCCA
    CACCCATCCC CGTTCATGCA AAGAAGGCAG CTGCACGGCG AGAGCCCAAC CCGGAGGTTG
    CAAAGATCCA AAAGGATAAG CGACAGCGAA GTGTCTGACT ACGACTGTGA TGATGGCGTT
    GGTGTTGTTT CAGACTTTCG GCATAATGGG CGTGACCTGC AGAGTTCAAC GTTATCTGTT
    CCGGAACAAG TAATGTCGTC CAATCACTGT TCCCGGACTG GGCCCCCGCA TGTAGACAGC
    ATAGGAAGAA CGAGGTCATG GTCTCCCAGT GTCCCTCCTC CGCAAAGTAG GAATTTGGAA
    CAGGGACCGA GAGGGACAAG ATCTGGCCCT GCACATTACA ATACCATGAG CAGAGTGGAA
    AGGCATCGAG TATTAGATAA CCACTACTCC CCAGACAGAG ACAGGAATTG TGAAGCGGCA
    GATAGACAGC CATATCAAAG ATCCAGGTCC ACTGAACAAC GGCCTGTGCT GGAGCGGACC
    AGCTCCCGCT CCCGATCTAC TGAGCGTCCT GACTCAAACC TCATGAGGTC GATGCCTTCA
    TTAATGACGG GAAGATCTGC CCCTCCTTCA CCCGCCTTAT CGAGGGCACA CCCTCCTCGT
    ACCGGCTCTG TTCAGACAAG TCCTTCAGGC ACCCCGTTAG CAGGGCGGAG GGGGCGCCAG
    CTCCCGCAAC TTCCCCCAAA AGGCACGTTA GAAAGAAAAA ATGGAGACAA GGAAATAGAG
    CTTTATGAAG GTGCTATGGA CGTAGAAGAA AGGACGCGAC AGCTGAAAGT GAAGATCAAC
    AAATACAAGC AGGTGGGAGG ATCAGATTCT CGGCTAGAGA AGGATTATCA TCCAAAGTAT
    CGGACGGGGC GGGATCCGCA GAGGGGCTCA GACAATATGT CCAACAAGTC ATCAGACAGC
    GATGTTAGTG ATGTGTCTGC CGTATCACGG ACCAGCAGCG CCTCTCGTTT TAGTAGTACG
    AGTTATATGT CTGTGCAGTC GGAAAGGCCT CGAGGGAACA TGAAAATAGG TAGAAGGACA
    AGGGAAGTAG AGGAGAGAAT AGAGAGGGCT GAGGGAGGGC AGCATCACAA AGAAATAGGT
    TTTCTGGAGG AAGAGGAGGA CGTTAGGCAG GAAGTAGATA GTGAGAAGGA AACAGAAGTG
    CAGCATATAA TTGACAACTG CATGAAGGGG CCAAGCAAAG AAAGTGTAGA GGAAGAGAGT
    CAACAGAATG AGAAAGATGA GGAAAGCCAT GATGTTGAGG AAGCCCATAA TGAGAATCTG
    CCAAGGAGGT CCTCAGAAAA TGATATAAGT GCATTGGCAT CCAAAATGCA AAGCAGGCAA
    ATGGGCGCCT CAGGGAAGAA CATGACCAAA AGTACCAGCA TCGGAGGGGA TATGTACACC
    CTGGAGAAGA ACGACGGAAG CCAGTCTGAC ACAGCCGTGG GCACTGTGGG TGCGGGTGGA
    AAGAAGAGAC GTTCAAGCAT AGGCGCCAAA ATGGTGGCCA TTGTGGGCTT GTCACGAAAA
    AGCCGAAGTA CCTCACAGCT CAGCCAAACA GAAGTCGGAG GGAAGAAGCT GAAGAGCACC
    ATCCAGAGAA GTACGGAAAC AGGCCTGGCC GTGGAAATGA GAAACTGGAT GAGCCGCCAA
    GCAAGCAGGG AGTCCACAGA TGGGAGTATG AACAGCTACA GCTCAGAGGG GAATCTGATA
    TTCCCTGGGG TCCGACTGGG TTCCGACAGC CAGTTCAGTG ACTTTTTGGA TGGTCTTGGT
    CCTGCCCAGC TGGTTGGTCG CCAGACTTTG GCCACGCCCT CTATGGGAGA TATTCAGGTT
    GGAATGGCTG ACAAAAAAGG CCAGTTGGAG GTGGAGGTGA TACGAGCCCG TGGTCTTGTT
    GTAAAACCAG GCTCAAAGAC ACTGCCAGCA CCCTATGTTA AGGTGTATCT CTTAGAAAAT
    GGAGTGTGCA TAGCCAAAAA GAAAACAAAA GTAGCAAGAA AAACGCTGGA GCCTCTATAC
    CAGCAAGTGT TAGCGTTTGA GGAATCACCA CAAGGGAAGG TCCTACAGAT CATTGTTTGG
    GGTGACTATG GACGCATGGA CCACAAGTCC TTCATGGGGG TGGCTCAGAT ACTTTTAGAT
    GAACTGGACC TATCCAGCAT GGTGATTGGA TGGTATAAAC TTTTCCCCCC TTCATCTTTA
    GTAGACCCAA CGTTGGCTCC ACTCACTAGG AGAGCTTCCC AATCATCTCT TGAAAGCTCC
    ACGGGACCTT CTTATGCTCG TTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031759429.1
    CDS419..5245
    Translation

    Target ORF information:

    RefSeq Version XM_031903569.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 2 (rims2), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031903569.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTCTGCTC CACTAGGGCC CAGGGGCGGC CCTCTGCCTC CTGCACAGCC ACAGGCTCCA 
    ATGCCAGAGA TGCCGGACCT CAGCCACCTG ACAGAGGATG AGAGGAAAAT CATAATGGCT
    GTGATGGAGA GGCAGAAGAA AGAAGAGGAG AAGGAGCAGT CAGTGTTAAA AAAATTGCAC
    CAGCAGTTTG AAATGTACAA GGAGCAAGTG AAGAAGATGG GCGAGGAAGC ACAGCAGCAG
    CAGGAGCAGA AGGGCGAAGC TCCAACCTGT GGAATCTGCC ACAAAACAAA GTTTGCTGAT
    GGCTGTGGCC ACGACTGTTC GTATTGCCAA ACTAAATTCT GCGCGCGGTG TGGAGGGCGA
    GTGTCTCTGC GCTCAAATAA GGAGGACAAA GTGGTAATGT GGGTATGTAA TTTGTGCCGA
    AAACAACAAG AAATCCTCAC CAAATCAGGG GCCTGGTTTT ACAGCAGTGG GTCAAGCACA
    CCCCTAAAGC CCGGCGAAGA GAGCCAGGCA GGGTACCAAA CGGAAGAAGC ACCTCAGGAG
    AAAAAAGCTA AGCTACAAGA GCAGTCCCAA TACCAGGGAC CCTCAGGGGA CTTATTAACA
    CCAGCCTCGG ACAAAAACCG ACCGCAAGGG ATCGTCCGGC AAGATTCCAT CAAAAATGGT
    TCCGAACTGA AGCACCCGAT TCCTGGCGAC CTCGCATCTG ACAGGAAAAG AAGCCCTTCA
    ATACCTAGAG ACCAGAATCG GAGATATGAT ACAAGGGACG ATATAGGTGA ACATTCACAG
    CATGCTACAT CAGACAGCGC CATGCCTAGA TCACCATCAG ACTACTCGGA CAGGCGATTG
    CAACGAGGGT CTCAGCTCTA TGATGAGCCT GAGCACGGTG ACTACAGAAG AAGTAGGCGG
    CGCTCCAATG AGTATTCGGT AGATGAGGAG GATATCCAAG ACAGGGAGGA ATATGAAAGG
    CAGAGAAGAG AGGAGGAGTA CCAGGCAAGG TACCGGAGTG ACCCCAACTT GGCCAGGTAC
    CCTGTTAAGC CACAACCGTA TGAAGAGCAA ATGCGCATCC ATGCAGAGGT TTCAAGGGCT
    CGACAACGAA GGCACAGCGA TGTGTCTTTA GCTCAGACAG AATTTGAGGA GTCTCGGATG
    TCAACGCTGA GGATGGAGAG ACCTTCAAGG CAAAGATCTG CGTCAGAGCG TAGAGCAGCG
    ATGGAAAGCC AGCGTTCATA TTCAATAGAA AGAACTAGAG ATGTGCAAGG GCCAGGTCCT
    GGTCGGCAAA GGACCTCAAA TCATAGTCCC CCAACACCAA GAAGGAGTCC CATTCCCAAT
    GATAGGCCAG ATATGAGGCG CACAGATTCT TTACGGAAAC AGCACCACTT GGACCCAAGT
    TCTGCCGTAA GGAAAACCAA AAGGGAAAAA ATGGAAACTA TGTTAAGGAA TGATTCTCTT
    AGCTCAGATC AGTCTGAATC TGTCCGGCCC CCACCACCAA AGCCTCATAA AACAAAAAAG
    GGAGGTAAAA TGCGTCAGGT TTCCTTGAGC AGTTCAGAAG AAGAGTTGGC GTCTACGCCG
    GAGTACACGA GCTGCGATGA TGTGGAGATT GAAAGTGAAA GTGTTAGTGA GAAAGGTGAC
    ATGGACTACA ACTGGGTGGA GCATTCGTCT TGGCACAGCA GTGAGGCCTC CCCAATGTCT
    TTGCACCCTG TCACGTGGCA ACCTTCCAAA GATGGAGACC GCTTAATTGG TCGCATATTA
    TTAAACAAGC GGCTCAAAGA TGGGAGCGTC CCGCGAGATT CTGGAGCCAT GCTGGGGCTG
    AAGGTCGTTG GAGGGAAAAT GACAGAATCA GGTCGACTTT GTGCGTTTAT CACCAAAGTG
    AAGAAGGGAA GCCTGGCAGA TACAGTAGGC CATCTTCGAC CAGGTGATGA GGTTTTAGAA
    TGGAGTGGGC GGATTTTGCA AGGAGCGACG TTCGAAGAAG TTTACAATAT TATACTGGAG
    TCCAAACCCG AGGCACAAGT AGAATTATTG GTCTCCAGAC CCATAGGAGA TATACCCCGG
    ATTCCTGACA GCACACACGC ACAACTGGAA TCCAGTTCTA GCTCATTTGA ATCCCAGAAA
    ATGGATCGCC CTTCTATTTC AGTTACTTCC CCCATGAGTC CTGGAATGTT ACGGGATGTC
    CCCCAGTTTC TTTCTGGACA ACTTTCAATT AAACTCTGGT ATGACAAGGT TGGCCACCAG
    TTAATTGTCA CGATATTAGG AGCAAAGGAT CTGCCATCGC GGGAAGATGG GAGGCCGAGA
    AATCCTTACG TAAAAATTTA CTTTCTTCCA GATCGAAGTG ATAAGAACAA AAGGAGAACA
    AAGACTGTGA AGAAAACCGT AGAGCCCAAA TGGAACCAAA CATTCATCTA TTCTCCGGTT
    CATCGGAGGG AATTTCGGGA ACGAATGTTG GAAATAACAC TTTGGGATCA AGCTCGGGTT
    CGAGAAGAAG AGAGTGAATT TTTAGGAGAG ATTCTAATTG AATTAGAAAC TGCACTATTA
    GATGATGAGC CACATTGGTA CAAGCTACAG ACGCATGATG TGTCTTCATT GCCACTTCCA
    CACCCATCCC CGTTCATGCA AAGAAGGCAG CTGCACGGCG AGAGCCCAAC CCGGAGGTTG
    CAAAGATCCA AAAGGATAAG CGACAGCGAA GTGTCTGACT ACGACTGTGA TGATGGCGTT
    GGTGTTGTTT CAGACTTTCG GCATAATGGG CGTGACCTGC AGAGTTCAAC GTTATCTGTT
    CCGGAACAAG TAATGTCGTC CAATCACTGT TCCCGGACTG GGCCCCCGCA TGTAGACAGC
    ATAGGAAGAA CGAGGTCATG GTCTCCCAGT GTCCCTCCTC CGCAAAGTAG GAATTTGGAA
    CAGGGACCGA GAGGGACAAG ATCTGGCCCT GCACATTACA ATACCATGAG CAGAGTGGAA
    AGGCATCGAG TATTAGATAA CCACTACTCC CCAGACAGAG ACAGGAATTG TGAAGCGGCA
    GATAGACAGC CATATCAAAG ATCCAGGTCC ACTGAACAAC GGCCTGTGCT GGAGCGGACC
    AGCTCCCGCT CCCGATCTAC TGAGCGTCCT GACTCAAACC TCATGAGGTC GATGCCTTCA
    TTAATGACGG GAAGATCTGC CCCTCCTTCA CCCGCCTTAT CGAGGGCACA CCCTCCTCGT
    ACCGGCTCTG TTCAGACAAG TCCTTCAGGC ACCCCGTTAG CAGGGCGGAG GGGGCGCCAG
    CTCCCGCAAC TTCCCCCAAA AGGCACGTTA GAAAGAAAAA ATGGAGACAA GGAAATAGAG
    CTTTATGAAG GTGCTATGGA CGTAGAAGAA AGGACGCGAC AGCTGAAAGT GAAGATCAAC
    AAATACAAGC AGGTGGGAGG ATCAGATTCT CGGCTAGAGA AGGATTATCA TCCAAAGTAT
    CGGACGGGGC GGGATCCGCA GAGGGGCTCA GACAATATGT CCAACAAGTC ATCAGACAGC
    GATGTTAGTG ATGTGTCTGC CGTATCACGG ACCAGCAGCG CCTCTCGTTT TAGTAGTACG
    AGTTATATGT CTGTGCAGTC GGAAAGGCCT CGAGGGAACA TGAAAATAGG TAGAAGGACA
    AGGGAAGTAG AGGAGAGAAT AGAGAGGGCT GAGGGAGGGC AGCATCACAA AGAAATAGGT
    TTTCTGGAGG AAGAGGAGGA CGTTAGGCAG GAAGTAGATA GTGAGAAGGA AACAGAAGTG
    CAGCATATAA TTGACAACTG CATGAAGGGG CCAAGCAAAG AAAGTGTAGA GGAAGAGAGT
    CAACAGAATG AGAAAGATGA GGAAAGCCAT GATGTTGAGG AAGCCCATAA TGAGAATCTG
    CCAAGGAGGT CCTCAGAAAA TGATATAAGT GCATTGGCAT CCAAAATGCA AAGCAGGCAA
    ATGGGCGCCT CAGGGAAGAA CATGACCAAA AGTACCAGCA TCGGAGGGGA TATGTACACC
    CTGGAGAAGA ACGACGGAAG CCAGTCTGAC ACAGCCGTGG GCACTGTGGG TGCGGGTGGA
    AAGAAGAGAC GTTCAAGCAT AGGCGCCAAA ATGGTGGCCA TTGTGGGCTT GTCACGAAAA
    AGCCGAAGTA CCTCACAGCT CAGCCAAACA GAAGTCGGAG GGAAGAAGCT GAAGAGCACC
    ATCCAGAGAA GTACGGAAAC AGGCCTGGCC GTGGAAATGA GAAACTGGAT GAGCCGCCAA
    GCAAGCAGGG AGTCCACAGA TGGGAGTATG AACAGCTACA GCTCAGAGGG GAATCTGATA
    TTCCCTGGGG TCCGACTGGG TTCCGACAGC CAGTTCAGTG ACTTTTTGGA TGGTCTTGGT
    CCTGCCCAGC TGGTTGGTCG CCAGACTTTG GCCACGCCCT CTATGGGAGA TATTCAGGTT
    GGAATGGCTG ACAAAAAAGG CCAGTTGGAG GTGGAGGTGA TACGAGCCCG TGGTCTTGTT
    GTAAAACCAG GCTCAAAGAC ACTGCCAGCA CCCTATGTTA AGGTGTATCT CTTAGAAAAT
    GGAGTGTGCA TAGCCAAAAA GAAAACAAAA GTAGCAAGAA AAACGCTGGA GCCTCTATAC
    CAGCAAGTGT TAGCGTTTGA GGAATCACCA CAAGGGAAGG TCCTACAGAT CATTGTTTGG
    GGTGACTATG GACGCATGGA CCACAAGTCC TTCATGGGGG TGGCTCAGAT ACTTTTAGAT
    GAACTGGACC TATCCAGCAT GGTGATTGGA TGGTATAAAC TTTTCCCCCC TTCATCTTTA
    GTAGACCCAA CGTTGGCTCC ACTCACTAGG AGAGCTTCCC AATCATCTCT TGAAAGCTCC
    ACGGGACCTT CTTATGCTCG TTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa59913
    Clone ID Related Accession (Same CDS sequence) XM_031903571.1
    Accession Version XM_031903571.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4626bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 2 isoform X25
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTCTGCTC CACTAGGGCC CAGGGGCGGC CCTCTGCCTC CTGCACAGCC ACAGGCTCCA 
    ATGCCAGAGA TGCCGGACCT CAGCCACCTG ACAGAGGATG AGAGGAAAAT CATAATGGCT
    GTGATGGAGA GGCAGAAGAA AGAAGAGGAG AAGGAGCAGT CAGTGTTAAA GGTCAAAGAA
    GAACAAAAAG CCCAGCCGGC ACAGTGGTTT CCCTTTAGTG GGATCACTGA GCTGGTTAAT
    AACGTTCTTC AGCCACAGCC AAAAGAGCAA AATGAAAAGG AGCCGGAGAT AAAATTGCAC
    CAGCAGTTTG AAATGTACAA GGAGCAAGTG AAGAAGATGG GCGAGGAAGC ACAGCAGCAG
    CAGGAGCAGA AGGGCGAAGC TCCAACCTGT GGAATCTGCC ACAAAACAAA GTTTGCTGAT
    GGCTGTGGCC ACGACTGTTC GTATTGCCAA ACTAAATTCT GCGCGCGGTG TGGAGGGCGA
    GTGTCTCTGC GCTCAAATAA GGAGGACAAA GTGGTAATGT GGGTATGTAA TTTGTGCCGA
    AAACAACAAG AAATCCTCAC CAAATCAGGG GCCTGGTTTT ACAGCAGTGG GTCAAGCACA
    CCCCTAAAGC CCGGCGAAGA GAGCCAGGCA GGGTACCAAA CGGAAGAAGC ACCTCAGGAG
    AAAAAAGCTA AGCTACAAGA GCAGTCCCAA TACCAGGGAC CCTCAGGGGA CTTATTAACA
    CCAGCCTCGG ACAAAAACCG ACCGCAAGGG ATCGTCCGGC AAGATTCCAT CAAAAATGGT
    TCCGAACTGA AGCACCCGAT TCCTGGCGAC CTCGCATCTG ACAGGAAAAG AAGCCCTTCA
    ATACCTAGAG ACCAGAATCG GAGATATGAT ACAAGGGACG ATATAGGTGA ACATTCACAG
    CATGCTACAT CAGACAGCGC CATGCCTAGA TCACCATCAG ACTACTCGGA CAGGCGATTG
    CAACGAGGGT CTCAGCTCTA TGATGAGCCT GAGCACGGTG ACTACAGAAG AAGTAGGCGG
    CGCTCCAATG AGTATTCGGT AGATGAGGAG GATATCCAAG ACAGGGAGGA ATATGAAAGG
    CAGAGAAGAG AGGAGGAGTA CCAGGCAAGG TACCGGAGTG ACCCCAACTT GGCCAGGTAC
    CCTGTTAAGC CACAACCGTA TGAAGAGCAA ATGCGCATCC ATGCAGAGGT TTCAAGGGCT
    CGACAACGAA GGCACAGCGA TGTGTCTTTA GCTCAGACAG AATTTGAGGA GTCTCGGATG
    TCAACGCTGA GGATGGAGAG ACCTTCAAGG CAAAGATCTG CGTCAGAGCG TAGAGCAGCG
    ATGGAAAGCC AGCGTTCATA TTCAATAGAA AGAACTAGAG ATGTGCAAGG GCCAGGTCCT
    GGTCGGCAAA GGACCTCAAA TCATAGTCCC CCAACACCAA GAAGGAGTCC CATTCCCAAT
    GATAGGCCAG ATATGAGGCG CACAGATTCT TTACGGAAAC AGCACCACTT GGACCCAAGT
    TCTGCCGTAA GGAAAACCAA AAGGGAAAAA ATGGAAACTA TGTTAAGGAA TGATTCTCTT
    AGCTCAGATC AGTCTGAATC TGTCCGGCCC CCACCACCAA AGCCTCATAA AACAAAAAAG
    GGAGGTAAAA TGCGTCAGGT TTCCTTGAGC AGTTCAGAAG AAGAGTTGGC GTCTACGCCG
    GAGTACACGA GCTGCGATGA TGTGGAGATT GAAAGTGAAA GTGTTAGTGA GAAAGGGGAC
    AGTCAAAGGG GAAAAAGAAA AACTAGTGAG CAGGCAGTTT TGTCGGATTC TAACATCTTG
    TCTGAGAGAC AAAAGAAAAT GGTGTGCTTT GGAGGCCACT CTTTGGAAGA GGATTTGGAA
    TGGTCTGAGC CTCAGATTAA AGACTCTGGG GTAGACACGT GTAGTAGCAC CACCCTTAAC
    GAAGAGCATA GCCATAGCGA GAAGCACCCT GTCACGTGGC AACCTTCCAA AGATGGAGAC
    CGCTTAATTG GTCGCATATT ATTAAACAAG CGGCTCAAAG ATGGGAGCGT CCCGCGAGAT
    TCTGGAGCCA TGCTGGGGCT GAAGGTCGTT GGAGGGAAAA TGACAGAATC AGGTCGACTT
    TGTGCGTTTA TCACCAAAGT GAAGAAGGGA AGCCTGGCAG ATACAGTAGG CCATCTTCGA
    CCAGGTGATG AGGTTTTAGA ATGGAGTGGG CGGATTTTGC AAGGAGCGAC GTTCGAAGAA
    GTTTACAATA TTATACTGGA GTCCAAACCC GAGGCACAAG TAGAATTATT GGTCTCCAGA
    CCCATAGGAG ATATACCCCG GATTCCTGAC AGCACACACG CACAACTGGA ATCCAGTTCT
    AGCTCATTTG AATCCCAGAA AATGGATCGC CCTTCTATTT CAGTTACTTC CCCCATGAGT
    CCTGGAATGT TACGGGATGT CCCCCAGTTT CTTTCTGGAC AACTTTCAAG CCAAAGCCTT
    AATAGAAGAA CAGCGCCTTT TGTTCCTAGG GTTCAGATTA AACTCTGGTA TGACAAGGTT
    GGCCACCAGT TAATTGTCAC GATATTAGGA GCAAAGGATC TGCCATCGCG GGAAGATGGG
    AGGCCGAGAA ATCCTTACGT AAAAATTTAC TTTCTTCCAG ATCGAAGTGA TAAGAACAAA
    AGGAGAACAA AGACTGTGAA GAAAACCGTA GAGCCCAAAT GGAACCAAAC ATTCATCTAT
    TCTCCGGTTC ATCGGAGGGA ATTTCGGGAA CGAATGTTGG AAATAACACT TTGGGATCAA
    GCTCGGGTTC GAGAAGAAGA GAGTGAATTT TTAGGAGAGA TTCTAATTGA ATTAGAAACT
    GCACTATTAG ATGATGAGCC ACATTGGTAC AAGCTACAGA CGCATGATGT GTCTTCATTG
    CCACTTCCAC ACCCATCCCC GTTCATGCAA AGAAGGCAGC TGCACGGCGA GAGCCCAACC
    CGGAGGTTGC AAAATAAAGG CTCATATTCA TATAATTCAG GATCCAAAAG GATAAGCGAC
    AGCGAAGTGT CTGACTACGA CTGTGATGAT GGCGTTGGTG TTGTTTCAGA CTTTCGGCAT
    AATGGGCGTG ACCTGCAGAG TTCAACGTTA TCTGTTCCGG AACAAGTAAT GTCGTCCAAT
    CACTGTTCCC GGACTGGGCC CCCGCATGTA GACAGCATAG GAAGAACGAG GTCATGGTCT
    CCCAGTGTCC CTCCTCCGCA AAGTAGGAAT TTGGAACAGG GACCGAGAGG GACAAGATCT
    GGCCCTGCAC ATTACAATAC CATGAGCAGA GTGGAAAGGC ATCGAGTATT AGATAACCAC
    TACTCCCCAG ACAGAGACAG GAATTGTGAA GCGGCAGATA GACAGCCATA TCAAAGATCC
    AGGTCCACTG AACAACGGCC TGTGCTGGAG CGGACCAGCT CCCGCTCCCG ATCTACTGAG
    CGTCCTGACT CAAACCTCAT GAGGTCGATG CCTTCATTAA TGACGGGAAG ATCTGCCCCT
    CCTTCACCCG CCTTATCGAG GGCACACCCT CCTCGTACCG GCTCTGTTCA GACAAGTCCT
    TCAGGCACCC CGTTAGCAGG GCGGAGGGGG CGCCAGCTCC CGCAACTTCC CCCAAAAGGC
    ACGTTAGAAA GAAAAGTTAC TTGGGAAGAC CAGCAAAGAA AGGTGAATGG TTCAATAACT
    GATGACAAAC ATTTGCCAAG AAAGAAACAG CATTCTGAGC CAGAAGATAT GGACTCAACG
    CGGAGGCGAA ACTCAGAGGA AAAGGCTGCA GAACAAGAGA ATGGGAACCC AGGTGCTATG
    GACGTAGAAG AAAGGACGCG ACAGCTGAAA GTGAAGATCA ACAAATACAA GCAGGTGGGA
    GGATCAGATT CTCGGCTAGA GAAGGATTAT CATCCAAAGT ATCGGACGGG GCGGGATCCG
    CAGAGGGGCT CAGACAATAT GTCCAACAAG TCATCAGACA GCGATGTTAG TGATGTGTCT
    GCCGTATCAC GGACCAGCAG CGCCTCTCGT TTTAGTAGTA CGAGTTATAT GTCTGTGCAG
    TCGGAAAGGC CTCGAGGGAA CATGAAAATA GGTCTGATAT TCCCTGGGGT CCGACTGGGT
    TCCGACAGCC AGTTCAGTGA CTTTTTGGAT GGTCTTGGTC CTGCCCAGCT GGTTGGTCGC
    CAGACTTTGG CCACGCCCTC TATGGGAGAT ATTCAGGTTG GAATGGCTGA CAAAAAAGGC
    CAGTTGGAGG TGGAGGTGAT ACGAGCCCGT GGTCTTGTTG TAAAACCAGG CTCAAAGACA
    CTGCCAGCAC CCTATGTTAA GGTGTATCTC TTAGAAAATG GAGTGTGCAT AGCCAAAAAG
    AAAACAAAAG TAGCAAGAAA AACGCTGGAG CCTCTATACC AGCAAGTGTT AGCGTTTGAG
    GAATCACCAC AAGGGAAGGT CCTACAGATC ATTGTTTGGG GTGACTATGG ACGCATGGAC
    CACAAGTCCT TCATGGGGGT GGCTCAGATA CTTTTAGATG AACTGGACCT ATCCAGCATG
    GTGATTGGAT GGTATAAACT TTTCCCCCCT TCATCTTTAG TAGACCCAAC GTTGGCTCCA
    CTCACTAGGA GAGCTTCCCA ATCATCTCTT GAAAGCTCCA CGGGACCTTC TTATGCTCGT
    TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031759431.1
    CDS423..5048
    Translation

    Target ORF information:

    RefSeq Version XM_031903571.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 2 (rims2), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031903571.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTCTGCTC CACTAGGGCC CAGGGGCGGC CCTCTGCCTC CTGCACAGCC ACAGGCTCCA 
    ATGCCAGAGA TGCCGGACCT CAGCCACCTG ACAGAGGATG AGAGGAAAAT CATAATGGCT
    GTGATGGAGA GGCAGAAGAA AGAAGAGGAG AAGGAGCAGT CAGTGTTAAA GGTCAAAGAA
    GAACAAAAAG CCCAGCCGGC ACAGTGGTTT CCCTTTAGTG GGATCACTGA GCTGGTTAAT
    AACGTTCTTC AGCCACAGCC AAAAGAGCAA AATGAAAAGG AGCCGGAGAT AAAATTGCAC
    CAGCAGTTTG AAATGTACAA GGAGCAAGTG AAGAAGATGG GCGAGGAAGC ACAGCAGCAG
    CAGGAGCAGA AGGGCGAAGC TCCAACCTGT GGAATCTGCC ACAAAACAAA GTTTGCTGAT
    GGCTGTGGCC ACGACTGTTC GTATTGCCAA ACTAAATTCT GCGCGCGGTG TGGAGGGCGA
    GTGTCTCTGC GCTCAAATAA GGAGGACAAA GTGGTAATGT GGGTATGTAA TTTGTGCCGA
    AAACAACAAG AAATCCTCAC CAAATCAGGG GCCTGGTTTT ACAGCAGTGG GTCAAGCACA
    CCCCTAAAGC CCGGCGAAGA GAGCCAGGCA GGGTACCAAA CGGAAGAAGC ACCTCAGGAG
    AAAAAAGCTA AGCTACAAGA GCAGTCCCAA TACCAGGGAC CCTCAGGGGA CTTATTAACA
    CCAGCCTCGG ACAAAAACCG ACCGCAAGGG ATCGTCCGGC AAGATTCCAT CAAAAATGGT
    TCCGAACTGA AGCACCCGAT TCCTGGCGAC CTCGCATCTG ACAGGAAAAG AAGCCCTTCA
    ATACCTAGAG ACCAGAATCG GAGATATGAT ACAAGGGACG ATATAGGTGA ACATTCACAG
    CATGCTACAT CAGACAGCGC CATGCCTAGA TCACCATCAG ACTACTCGGA CAGGCGATTG
    CAACGAGGGT CTCAGCTCTA TGATGAGCCT GAGCACGGTG ACTACAGAAG AAGTAGGCGG
    CGCTCCAATG AGTATTCGGT AGATGAGGAG GATATCCAAG ACAGGGAGGA ATATGAAAGG
    CAGAGAAGAG AGGAGGAGTA CCAGGCAAGG TACCGGAGTG ACCCCAACTT GGCCAGGTAC
    CCTGTTAAGC CACAACCGTA TGAAGAGCAA ATGCGCATCC ATGCAGAGGT TTCAAGGGCT
    CGACAACGAA GGCACAGCGA TGTGTCTTTA GCTCAGACAG AATTTGAGGA GTCTCGGATG
    TCAACGCTGA GGATGGAGAG ACCTTCAAGG CAAAGATCTG CGTCAGAGCG TAGAGCAGCG
    ATGGAAAGCC AGCGTTCATA TTCAATAGAA AGAACTAGAG ATGTGCAAGG GCCAGGTCCT
    GGTCGGCAAA GGACCTCAAA TCATAGTCCC CCAACACCAA GAAGGAGTCC CATTCCCAAT
    GATAGGCCAG ATATGAGGCG CACAGATTCT TTACGGAAAC AGCACCACTT GGACCCAAGT
    TCTGCCGTAA GGAAAACCAA AAGGGAAAAA ATGGAAACTA TGTTAAGGAA TGATTCTCTT
    AGCTCAGATC AGTCTGAATC TGTCCGGCCC CCACCACCAA AGCCTCATAA AACAAAAAAG
    GGAGGTAAAA TGCGTCAGGT TTCCTTGAGC AGTTCAGAAG AAGAGTTGGC GTCTACGCCG
    GAGTACACGA GCTGCGATGA TGTGGAGATT GAAAGTGAAA GTGTTAGTGA GAAAGGGGAC
    AGTCAAAGGG GAAAAAGAAA AACTAGTGAG CAGGCAGTTT TGTCGGATTC TAACATCTTG
    TCTGAGAGAC AAAAGAAAAT GGTGTGCTTT GGAGGCCACT CTTTGGAAGA GGATTTGGAA
    TGGTCTGAGC CTCAGATTAA AGACTCTGGG GTAGACACGT GTAGTAGCAC CACCCTTAAC
    GAAGAGCATA GCCATAGCGA GAAGCACCCT GTCACGTGGC AACCTTCCAA AGATGGAGAC
    CGCTTAATTG GTCGCATATT ATTAAACAAG CGGCTCAAAG ATGGGAGCGT CCCGCGAGAT
    TCTGGAGCCA TGCTGGGGCT GAAGGTCGTT GGAGGGAAAA TGACAGAATC AGGTCGACTT
    TGTGCGTTTA TCACCAAAGT GAAGAAGGGA AGCCTGGCAG ATACAGTAGG CCATCTTCGA
    CCAGGTGATG AGGTTTTAGA ATGGAGTGGG CGGATTTTGC AAGGAGCGAC GTTCGAAGAA
    GTTTACAATA TTATACTGGA GTCCAAACCC GAGGCACAAG TAGAATTATT GGTCTCCAGA
    CCCATAGGAG ATATACCCCG GATTCCTGAC AGCACACACG CACAACTGGA ATCCAGTTCT
    AGCTCATTTG AATCCCAGAA AATGGATCGC CCTTCTATTT CAGTTACTTC CCCCATGAGT
    CCTGGAATGT TACGGGATGT CCCCCAGTTT CTTTCTGGAC AACTTTCAAG CCAAAGCCTT
    AATAGAAGAA CAGCGCCTTT TGTTCCTAGG GTTCAGATTA AACTCTGGTA TGACAAGGTT
    GGCCACCAGT TAATTGTCAC GATATTAGGA GCAAAGGATC TGCCATCGCG GGAAGATGGG
    AGGCCGAGAA ATCCTTACGT AAAAATTTAC TTTCTTCCAG ATCGAAGTGA TAAGAACAAA
    AGGAGAACAA AGACTGTGAA GAAAACCGTA GAGCCCAAAT GGAACCAAAC ATTCATCTAT
    TCTCCGGTTC ATCGGAGGGA ATTTCGGGAA CGAATGTTGG AAATAACACT TTGGGATCAA
    GCTCGGGTTC GAGAAGAAGA GAGTGAATTT TTAGGAGAGA TTCTAATTGA ATTAGAAACT
    GCACTATTAG ATGATGAGCC ACATTGGTAC AAGCTACAGA CGCATGATGT GTCTTCATTG
    CCACTTCCAC ACCCATCCCC GTTCATGCAA AGAAGGCAGC TGCACGGCGA GAGCCCAACC
    CGGAGGTTGC AAAATAAAGG CTCATATTCA TATAATTCAG GATCCAAAAG GATAAGCGAC
    AGCGAAGTGT CTGACTACGA CTGTGATGAT GGCGTTGGTG TTGTTTCAGA CTTTCGGCAT
    AATGGGCGTG ACCTGCAGAG TTCAACGTTA TCTGTTCCGG AACAAGTAAT GTCGTCCAAT
    CACTGTTCCC GGACTGGGCC CCCGCATGTA GACAGCATAG GAAGAACGAG GTCATGGTCT
    CCCAGTGTCC CTCCTCCGCA AAGTAGGAAT TTGGAACAGG GACCGAGAGG GACAAGATCT
    GGCCCTGCAC ATTACAATAC CATGAGCAGA GTGGAAAGGC ATCGAGTATT AGATAACCAC
    TACTCCCCAG ACAGAGACAG GAATTGTGAA GCGGCAGATA GACAGCCATA TCAAAGATCC
    AGGTCCACTG AACAACGGCC TGTGCTGGAG CGGACCAGCT CCCGCTCCCG ATCTACTGAG
    CGTCCTGACT CAAACCTCAT GAGGTCGATG CCTTCATTAA TGACGGGAAG ATCTGCCCCT
    CCTTCACCCG CCTTATCGAG GGCACACCCT CCTCGTACCG GCTCTGTTCA GACAAGTCCT
    TCAGGCACCC CGTTAGCAGG GCGGAGGGGG CGCCAGCTCC CGCAACTTCC CCCAAAAGGC
    ACGTTAGAAA GAAAAGTTAC TTGGGAAGAC CAGCAAAGAA AGGTGAATGG TTCAATAACT
    GATGACAAAC ATTTGCCAAG AAAGAAACAG CATTCTGAGC CAGAAGATAT GGACTCAACG
    CGGAGGCGAA ACTCAGAGGA AAAGGCTGCA GAACAAGAGA ATGGGAACCC AGGTGCTATG
    GACGTAGAAG AAAGGACGCG ACAGCTGAAA GTGAAGATCA ACAAATACAA GCAGGTGGGA
    GGATCAGATT CTCGGCTAGA GAAGGATTAT CATCCAAAGT ATCGGACGGG GCGGGATCCG
    CAGAGGGGCT CAGACAATAT GTCCAACAAG TCATCAGACA GCGATGTTAG TGATGTGTCT
    GCCGTATCAC GGACCAGCAG CGCCTCTCGT TTTAGTAGTA CGAGTTATAT GTCTGTGCAG
    TCGGAAAGGC CTCGAGGGAA CATGAAAATA GGTCTGATAT TCCCTGGGGT CCGACTGGGT
    TCCGACAGCC AGTTCAGTGA CTTTTTGGAT GGTCTTGGTC CTGCCCAGCT GGTTGGTCGC
    CAGACTTTGG CCACGCCCTC TATGGGAGAT ATTCAGGTTG GAATGGCTGA CAAAAAAGGC
    CAGTTGGAGG TGGAGGTGAT ACGAGCCCGT GGTCTTGTTG TAAAACCAGG CTCAAAGACA
    CTGCCAGCAC CCTATGTTAA GGTGTATCTC TTAGAAAATG GAGTGTGCAT AGCCAAAAAG
    AAAACAAAAG TAGCAAGAAA AACGCTGGAG CCTCTATACC AGCAAGTGTT AGCGTTTGAG
    GAATCACCAC AAGGGAAGGT CCTACAGATC ATTGTTTGGG GTGACTATGG ACGCATGGAC
    CACAAGTCCT TCATGGGGGT GGCTCAGATA CTTTTAGATG AACTGGACCT ATCCAGCATG
    GTGATTGGAT GGTATAAACT TTTCCCCCCT TCATCTTTAG TAGACCCAAC GTTGGCTCCA
    CTCACTAGGA GAGCTTCCCA ATCATCTCTT GAAAGCTCCA CGGGACCTTC TTATGCTCGT
    TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa59915
    Clone ID Related Accession (Same CDS sequence) XM_031903573.1
    Accession Version XM_031903573.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4581bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 2 isoform X27
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTCTGCTC CACTAGGGCC CAGGGGCGGC CCTCTGCCTC CTGCACAGCC ACAGGCTCCA 
    ATGCCAGAGA TGCCGGACCT CAGCCACCTG ACAGAGGATG AGAGGAAAAT CATAATGGCT
    GTGATGGAGA GGCAGAAGAA AGAAGAGGAG AAGGAGCAGT CAGTGTTAAA GGTCAAAGAA
    GAACAAAAAG CCCAGCCGGC ACAGTGGTTT CCCTTTAGTG GGATCACTGA GCTGGTTAAT
    AACGTTCTTC AGCCACAGCC AAAAGAGCAA AATGAAAAGG AGCCGGAGAT AAAATTGCAC
    CAGCAGTTTG AAATGTACAA GGAGCAAGTG AAGAAGATGG GCGAGGAAGC ACAGCAGCAG
    CAGGAGCAGA AGGGCGAAGC TCCAACCTGT GGAATCTGCC ACAAAACAAA GTTTGCTGAT
    GGCTGTGGCC ACGACTGTTC GTATTGCCAA ACTAAATTCT GCGCGCGGTG TGGAGGGCGA
    GTGTCTCTGC GCTCAAATAA GGTAATGTGG GTATGTAATT TGTGCCGAAA ACAACAAGAA
    ATCCTCACCA AATCAGGGGC CTGGTTTTAC AGCAGTGGGT CAAGCACACC CCTAAAGCCC
    GGCGAAGAGA GCCAGGCAGG GTACCAAACG GAAGAAGCAC CTCAGGAGAA AAAAGCTAAG
    CTACAAGAGC AGTCCCAATA CCAGGGACCC TCAGGGGACT TATTAACACC AGCCTCGGAC
    AAAAACCGAC CGCAAGGGAT CGTCCGGCAA GATTCCATCA AAAATGGTTC CGAACTGAAG
    CACCCGATTC CTGGCGACCT CGCATCTGAC AGGAAAAGAA GCCCTTCAAT ACCTAGAGAC
    CAGAATCGGA GATATGATAC AAGGGACGAT ATAGGTGAAC ATTCACAGCA TGCTACATCA
    GACAGCGCCA TGCCTAGATC ACCATCAGAC TACTCGGACA GGCGATTGCA ACGAGGGTCT
    CAGCTCTATG ATGAGCCTGA GCACGGTGAC TACAGAAGAA GTAGGCGGCG CTCCAATGAG
    TATTCGGTAG ATGAGGAGGA TATCCAAGAC AGGGAGGAAT ATGAAAGGCA GAGAAGAGAG
    GAGGAGTACC AGGCAAGGTA CCGGAGTGAC CCCAACTTGG CCAGGTACCC TGTTAAGCCA
    CAACCGTATG AAGAGCAAAT GCGCATCCAT GCAGAGGTTT CAAGGGCTCG ACAACGAAGG
    CACAGCGATG TGTCTTTAGC TCAGACAGAA TTTGAGGAGT CTCGGATGTC AACGCTGAGG
    ATGGAGAGAC CTTCAAGGCA AAGATCTGCG TCAGAGCGTA GAGCAGCGAT GGAAAGCCAG
    CGTTCATATT CAATAGAAAG AACTAGAGAT GTGCAAGGGC CAGGTCCTGG TCGGCAAAGG
    ACCTCAAATC ATAGTCCCCC AACACCAAGA AGGAGTCCCA TTCCCAATGA TAGGCCAGAT
    ATGAGGCGCA CAGATTCTTT ACGGAAACAG CACCACTTGG ACCCAAGTTC TGCCGTAAGG
    AAAACCAAAA GGGAAAAAAT GGAAACTATG TTAAGGAATG ATTCTCTTAG CTCAGATCAG
    TCTGAATCTG TCCGGCCCCC ACCACCAAAG CCTCATAAAA CAAAAAAGGG AGGTAAAATG
    CGTCAGGTTT CCTTGAGCAG TTCAGAAGAA GAGTTGGCGT CTACGCCGGA GTACACGAGC
    TGCGATGATG TGGAGATTGA AAGTGAAAGT GTTAGTGAGA AAGGGGACAG TCAAAGGGGA
    AAAAGAAAAA CTAGTGAGCA GGCAGTTTTG TCGGATTCTA ACATCTTGTC TGAGAGACAA
    AAGAAAATGG TGTGCTTTGG AGGCCACTCT TTGGAAGAGG ATTTGGAATG GTCTGAGCCT
    CAGATTAAAG ACTCTGGGGT AGACACGTGT AGTAGCACCA CCCTTAACGA AGAGCATAGC
    CATAGCGAGA AGCACCCTGT CACGTGGCAA CCTTCCAAAG ATGGAGACCG CTTAATTGGT
    CGCATATTAT TAAACAAGCG GCTCAAAGAT GGGAGCGTCC CGCGAGATTC TGGAGCCATG
    CTGGGGCTGA AGGTCGTTGG AGGGAAAATG ACAGAATCAG GTCGACTTTG TGCGTTTATC
    ACCAAAGTGA AGAAGGGAAG CCTGGCAGAT ACAGTAGGCC ATCTTCGACC AGGTGATGAG
    GTTTTAGAAT GGAGTGGGCG GATTTTGCAA GGAGCGACGT TCGAAGAAGT TTACAATATT
    ATACTGGAGT CCAAACCCGA GGCACAAGTA GAATTATTGG TCTCCAGACC CATAGGAGAT
    ATACCCCGGA TTCCTGACAG CACACACGCA CAACTGGAAT CCAGTTCTAG CTCATTTGAA
    TCCCAGAAAA TGGATCGCCC TTCTATTTCA GTTACTTCCC CCATGAGTCC TGGAATGTTA
    CGGGATGTCC CCCAGTTTCT TTCTGGACAA CTTTCAATTA AACTCTGGTA TGACAAGGTT
    GGCCACCAGT TAATTGTCAC GATATTAGGA GCAAAGGATC TGCCATCGCG GGAAGATGGG
    AGGCCGAGAA ATCCTTACGT AAAAATTTAC TTTCTTCCAG ATCGAAGTGA TAAGAACAAA
    AGGAGAACAA AGACTGTGAA GAAAACCGTA GAGCCCAAAT GGAACCAAAC ATTCATCTAT
    TCTCCGGTTC ATCGGAGGGA ATTTCGGGAA CGAATGTTGG AAATAACACT TTGGGATCAA
    GCTCGGGTTC GAGAAGAAGA GAGTGAATTT TTAGGAGAGA TTCTAATTGA ATTAGAAACT
    GCACTATTAG ATGATGAGCC ACATTGGTAC AAGCTACAGA CGCATGATGT GTCTTCATTG
    CCACTTCCAC ACCCATCCCC GTTCATGCAA AGAAGGCAGC TGCACGGCGA GAGCCCAACC
    CGGAGGTTGC AAAGATCCAA AAGGATAAGC GACAGCGAAG TGTCTGACTA CGACTGTGAT
    GATGGCGTTG GTGTTGTTTC AGACTTTCGG CATAATGGGC GTGACCTGCA GAGTTCAACG
    TTATCTGTTC CGGAACAAGT AATGTCGTCC AATCACTGTT CCCGGACTGG GCCCCCGCAT
    GTAGACAGCA TAGGAAGAAC GAGGTCATGG TCTCCCAGTG TCCCTCCTCC GCAAAGGAAT
    TTGGAACAGG GACCGAGAGG GACAAGATCT GGCCCTGCAC ATTACAATAC CATGAGCAGA
    GTGGAAAGGC ATCGAGTATT AGATAACCAC TACTCCCCAG ACAGAGACAG GGCACACCCT
    CCTCGTACCG GCTCTGTTCA GACAAGTCCT TCAGGCACCC CGTTAGCAGG GCGGAGGGGG
    CGCCAGCTCC CGCAACTTCC CCCAAAAGGC ACGTTAGAAA GAAGTGCTAT GGACGTAGAA
    GAAAGGACGC GACAGCTGAA AGTGAAGATC AACAAATACA AGCAGGTGGG AGGATCAGAT
    TCTCGGCTAG AGAAGGATTA TCATCCAAAG TATCGGACGG GGCGGGATCC GCAGAGGGGC
    TCAGACAATA TGTCCAACAA GTCATCAGAC AGCGATGTTA GTGATGTGTC TGCCGTATCA
    CGGACCAGCA GCGCCTCTCG TTTTAGTAGT ACGAGTTATA TGTCTGTGCA GTCGGAAAGG
    CCTCGAGGGA ACATGAAAAT AGGTGCATTG GCATCCAAAA TGCAAAGCAG GCAAATGGGC
    GCCTCAGGGA AGAACATGAC CAAAAGTACC AGCATCGGAG GGGATATGTA CACCCTGGAG
    AAGAACGACG GAAGCCAGTC TGACACAGCC GTGGGCACTG TGGGTGCGGG TGGAAAGAAG
    AGACGTTCAA GCATAGGCGC CAAAATGGTG GCCATTGTGG GCTTGTCACG AAAAAGCCGA
    AGTACCTCAC AGCTCAGCCA AACAGAAGTC GGAGGGAAGA AGCTGAAGAG CACCATCCAG
    AGAAGTACGG AAACAGGCCT GGCCGTGGAA ATGAGAAACT GGATGAGCCG CCAAGCAAGC
    AGGGAGTCCA CAGATGGGAG TATGAACAGC TACAGCTCAG AGGGGAATCT GATATTCCCT
    GGGGTCCGAC TGGGTTCCGA CAGCCAGTTC AGTGACTTTT TGGATGGTCT TGGTCCTGCC
    CAGCTGGTTG GTCGCCAGAC TTTGGCCACG CCCTCTATGG GAGATATTCA GGTTGGAATG
    GCTGACAAAA AAGGCCAGTT GGAGGTGGAG GTGATACGAG CCCGTGGTCT TGTTGTAAAA
    CCAGGCTCAA AGACACTGCC AGCACCCTAT GTTAAGGTGT ATCTCTTAGA AAATGGAGTG
    TGCATAGCCA AAAAGAAAAC AAAAGTAGCA AGAAAAACGC TGGAGCCTCT ATACCAGCAA
    GTGTTAGCGT TTGAGGAATC ACCACAAGGG AAGGTCCTAC AGATCATTGT TTGGGGTGAC
    TATGGACGCA TGGACCACAA GTCCTTCATG GGGGTGGCTC AGATACTTTT AGATGAACTG
    GACCTATCCA GCATGGTGAT TGGATGGTAT AAACTTTTCC CCCCTTCATC TTTAGTAGAC
    CCAACGTTGG CTCCACTCAC TAGGAGAGCT TCCCAATCAT CTCTTGAAAG CTCCACGGGA
    CCTTCTTATG CTCGTTCATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031759433.1
    CDS1..4581
    Translation

    Target ORF information:

    RefSeq Version XM_031903573.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 2 (rims2), transcript variant X27, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031903573.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTCTGCTC CACTAGGGCC CAGGGGCGGC CCTCTGCCTC CTGCACAGCC ACAGGCTCCA 
    ATGCCAGAGA TGCCGGACCT CAGCCACCTG ACAGAGGATG AGAGGAAAAT CATAATGGCT
    GTGATGGAGA GGCAGAAGAA AGAAGAGGAG AAGGAGCAGT CAGTGTTAAA GGTCAAAGAA
    GAACAAAAAG CCCAGCCGGC ACAGTGGTTT CCCTTTAGTG GGATCACTGA GCTGGTTAAT
    AACGTTCTTC AGCCACAGCC AAAAGAGCAA AATGAAAAGG AGCCGGAGAT AAAATTGCAC
    CAGCAGTTTG AAATGTACAA GGAGCAAGTG AAGAAGATGG GCGAGGAAGC ACAGCAGCAG
    CAGGAGCAGA AGGGCGAAGC TCCAACCTGT GGAATCTGCC ACAAAACAAA GTTTGCTGAT
    GGCTGTGGCC ACGACTGTTC GTATTGCCAA ACTAAATTCT GCGCGCGGTG TGGAGGGCGA
    GTGTCTCTGC GCTCAAATAA GGTAATGTGG GTATGTAATT TGTGCCGAAA ACAACAAGAA
    ATCCTCACCA AATCAGGGGC CTGGTTTTAC AGCAGTGGGT CAAGCACACC CCTAAAGCCC
    GGCGAAGAGA GCCAGGCAGG GTACCAAACG GAAGAAGCAC CTCAGGAGAA AAAAGCTAAG
    CTACAAGAGC AGTCCCAATA CCAGGGACCC TCAGGGGACT TATTAACACC AGCCTCGGAC
    AAAAACCGAC CGCAAGGGAT CGTCCGGCAA GATTCCATCA AAAATGGTTC CGAACTGAAG
    CACCCGATTC CTGGCGACCT CGCATCTGAC AGGAAAAGAA GCCCTTCAAT ACCTAGAGAC
    CAGAATCGGA GATATGATAC AAGGGACGAT ATAGGTGAAC ATTCACAGCA TGCTACATCA
    GACAGCGCCA TGCCTAGATC ACCATCAGAC TACTCGGACA GGCGATTGCA ACGAGGGTCT
    CAGCTCTATG ATGAGCCTGA GCACGGTGAC TACAGAAGAA GTAGGCGGCG CTCCAATGAG
    TATTCGGTAG ATGAGGAGGA TATCCAAGAC AGGGAGGAAT ATGAAAGGCA GAGAAGAGAG
    GAGGAGTACC AGGCAAGGTA CCGGAGTGAC CCCAACTTGG CCAGGTACCC TGTTAAGCCA
    CAACCGTATG AAGAGCAAAT GCGCATCCAT GCAGAGGTTT CAAGGGCTCG ACAACGAAGG
    CACAGCGATG TGTCTTTAGC TCAGACAGAA TTTGAGGAGT CTCGGATGTC AACGCTGAGG
    ATGGAGAGAC CTTCAAGGCA AAGATCTGCG TCAGAGCGTA GAGCAGCGAT GGAAAGCCAG
    CGTTCATATT CAATAGAAAG AACTAGAGAT GTGCAAGGGC CAGGTCCTGG TCGGCAAAGG
    ACCTCAAATC ATAGTCCCCC AACACCAAGA AGGAGTCCCA TTCCCAATGA TAGGCCAGAT
    ATGAGGCGCA CAGATTCTTT ACGGAAACAG CACCACTTGG ACCCAAGTTC TGCCGTAAGG
    AAAACCAAAA GGGAAAAAAT GGAAACTATG TTAAGGAATG ATTCTCTTAG CTCAGATCAG
    TCTGAATCTG TCCGGCCCCC ACCACCAAAG CCTCATAAAA CAAAAAAGGG AGGTAAAATG
    CGTCAGGTTT CCTTGAGCAG TTCAGAAGAA GAGTTGGCGT CTACGCCGGA GTACACGAGC
    TGCGATGATG TGGAGATTGA AAGTGAAAGT GTTAGTGAGA AAGGGGACAG TCAAAGGGGA
    AAAAGAAAAA CTAGTGAGCA GGCAGTTTTG TCGGATTCTA ACATCTTGTC TGAGAGACAA
    AAGAAAATGG TGTGCTTTGG AGGCCACTCT TTGGAAGAGG ATTTGGAATG GTCTGAGCCT
    CAGATTAAAG ACTCTGGGGT AGACACGTGT AGTAGCACCA CCCTTAACGA AGAGCATAGC
    CATAGCGAGA AGCACCCTGT CACGTGGCAA CCTTCCAAAG ATGGAGACCG CTTAATTGGT
    CGCATATTAT TAAACAAGCG GCTCAAAGAT GGGAGCGTCC CGCGAGATTC TGGAGCCATG
    CTGGGGCTGA AGGTCGTTGG AGGGAAAATG ACAGAATCAG GTCGACTTTG TGCGTTTATC
    ACCAAAGTGA AGAAGGGAAG CCTGGCAGAT ACAGTAGGCC ATCTTCGACC AGGTGATGAG
    GTTTTAGAAT GGAGTGGGCG GATTTTGCAA GGAGCGACGT TCGAAGAAGT TTACAATATT
    ATACTGGAGT CCAAACCCGA GGCACAAGTA GAATTATTGG TCTCCAGACC CATAGGAGAT
    ATACCCCGGA TTCCTGACAG CACACACGCA CAACTGGAAT CCAGTTCTAG CTCATTTGAA
    TCCCAGAAAA TGGATCGCCC TTCTATTTCA GTTACTTCCC CCATGAGTCC TGGAATGTTA
    CGGGATGTCC CCCAGTTTCT TTCTGGACAA CTTTCAATTA AACTCTGGTA TGACAAGGTT
    GGCCACCAGT TAATTGTCAC GATATTAGGA GCAAAGGATC TGCCATCGCG GGAAGATGGG
    AGGCCGAGAA ATCCTTACGT AAAAATTTAC TTTCTTCCAG ATCGAAGTGA TAAGAACAAA
    AGGAGAACAA AGACTGTGAA GAAAACCGTA GAGCCCAAAT GGAACCAAAC ATTCATCTAT
    TCTCCGGTTC ATCGGAGGGA ATTTCGGGAA CGAATGTTGG AAATAACACT TTGGGATCAA
    GCTCGGGTTC GAGAAGAAGA GAGTGAATTT TTAGGAGAGA TTCTAATTGA ATTAGAAACT
    GCACTATTAG ATGATGAGCC ACATTGGTAC AAGCTACAGA CGCATGATGT GTCTTCATTG
    CCACTTCCAC ACCCATCCCC GTTCATGCAA AGAAGGCAGC TGCACGGCGA GAGCCCAACC
    CGGAGGTTGC AAAGATCCAA AAGGATAAGC GACAGCGAAG TGTCTGACTA CGACTGTGAT
    GATGGCGTTG GTGTTGTTTC AGACTTTCGG CATAATGGGC GTGACCTGCA GAGTTCAACG
    TTATCTGTTC CGGAACAAGT AATGTCGTCC AATCACTGTT CCCGGACTGG GCCCCCGCAT
    GTAGACAGCA TAGGAAGAAC GAGGTCATGG TCTCCCAGTG TCCCTCCTCC GCAAAGGAAT
    TTGGAACAGG GACCGAGAGG GACAAGATCT GGCCCTGCAC ATTACAATAC CATGAGCAGA
    GTGGAAAGGC ATCGAGTATT AGATAACCAC TACTCCCCAG ACAGAGACAG GGCACACCCT
    CCTCGTACCG GCTCTGTTCA GACAAGTCCT TCAGGCACCC CGTTAGCAGG GCGGAGGGGG
    CGCCAGCTCC CGCAACTTCC CCCAAAAGGC ACGTTAGAAA GAAGTGCTAT GGACGTAGAA
    GAAAGGACGC GACAGCTGAA AGTGAAGATC AACAAATACA AGCAGGTGGG AGGATCAGAT
    TCTCGGCTAG AGAAGGATTA TCATCCAAAG TATCGGACGG GGCGGGATCC GCAGAGGGGC
    TCAGACAATA TGTCCAACAA GTCATCAGAC AGCGATGTTA GTGATGTGTC TGCCGTATCA
    CGGACCAGCA GCGCCTCTCG TTTTAGTAGT ACGAGTTATA TGTCTGTGCA GTCGGAAAGG
    CCTCGAGGGA ACATGAAAAT AGGTGCATTG GCATCCAAAA TGCAAAGCAG GCAAATGGGC
    GCCTCAGGGA AGAACATGAC CAAAAGTACC AGCATCGGAG GGGATATGTA CACCCTGGAG
    AAGAACGACG GAAGCCAGTC TGACACAGCC GTGGGCACTG TGGGTGCGGG TGGAAAGAAG
    AGACGTTCAA GCATAGGCGC CAAAATGGTG GCCATTGTGG GCTTGTCACG AAAAAGCCGA
    AGTACCTCAC AGCTCAGCCA AACAGAAGTC GGAGGGAAGA AGCTGAAGAG CACCATCCAG
    AGAAGTACGG AAACAGGCCT GGCCGTGGAA ATGAGAAACT GGATGAGCCG CCAAGCAAGC
    AGGGAGTCCA CAGATGGGAG TATGAACAGC TACAGCTCAG AGGGGAATCT GATATTCCCT
    GGGGTCCGAC TGGGTTCCGA CAGCCAGTTC AGTGACTTTT TGGATGGTCT TGGTCCTGCC
    CAGCTGGTTG GTCGCCAGAC TTTGGCCACG CCCTCTATGG GAGATATTCA GGTTGGAATG
    GCTGACAAAA AAGGCCAGTT GGAGGTGGAG GTGATACGAG CCCGTGGTCT TGTTGTAAAA
    CCAGGCTCAA AGACACTGCC AGCACCCTAT GTTAAGGTGT ATCTCTTAGA AAATGGAGTG
    TGCATAGCCA AAAAGAAAAC AAAAGTAGCA AGAAAAACGC TGGAGCCTCT ATACCAGCAA
    GTGTTAGCGT TTGAGGAATC ACCACAAGGG AAGGTCCTAC AGATCATTGT TTGGGGTGAC
    TATGGACGCA TGGACCACAA GTCCTTCATG GGGGTGGCTC AGATACTTTT AGATGAACTG
    GACCTATCCA GCATGGTGAT TGGATGGTAT AAACTTTTCC CCCCTTCATC TTTAGTAGAC
    CCAACGTTGG CTCCACTCAC TAGGAGAGCT TCCCAATCAT CTCTTGAAAG CTCCACGGGA
    CCTTCTTATG CTCGTTCATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa59917
    Clone ID Related Accession (Same CDS sequence) XM_031903575.1
    Accession Version XM_031903575.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4524bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 2 isoform X29
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCATTCGC ATGAAAATGA TGCCGGCTCT TTTCCAGATG TGCAATGGCT GTTTGCAAGG 
    AAAAGAAGCC CTTCAATACC TAGAGACCAG AATCGGAGAT ATGATACAAG GGACGATATA
    GGTGAACATT CACAGCATGC TACATCAGAC AGCGCCATGC CTAGATCACC ATCAGACTAC
    TCGGACAGGC GATTGCAACG AGGGTCTCAG CTCTATGATG AGCCTGAGCA CGGTGACTAC
    AGAAGAAGTA GGCGGCGCTC CAATGAGTAT TCGGTAGATG AGGAGGATAT CCAAGACAGG
    GAGGAATATG AAAGGCAGAG AAGAGAGGAG GAGTACCAGG CAAGGTACCG GAGTGACCCC
    AACTTGGCCA GGTACCCTGT TAAGCCACAA CCGTATGAAG AGCAAATGCG CATCCATGCA
    GAGGTTTCAA GGGCTCGACA ACGAAGGCAC AGCGATGTGT CTTTAGCTCA GACAGAATTT
    GAGGAGTCTC GGATGTCAAC GCTGAGGATG GAGAGACCTT CAAGGCAAAG ATCTGCGTCA
    GAGCGTAGAG CAGCGATGGA AAGCCAGCGT TCATATTCAA TAGAAAGAAC TAGAGATGTG
    CAAGGGCCAG GTCCTGGTCG GCAAAGGACC TCAAATCATA GTCCCCCAAC ACCAAGAAGG
    AGTCCCATTC CCAATGATAG GCCAGATATG AGGCGCACAG ATTCTTTACG GAAACAGCAC
    CACTTGGACC CAAGTTCTGC CGTAAGGAAA ACCAAAAGGG AAAAAATGGA AACTATGTTA
    AGGAATGATT CTCTTAGCTC AGATCAGTCT GAATCTGTCC GGCCCCCACC ACCAAAGCCT
    CATAAAACAA AAAAGGGAGG TAAAATGCGT CAGGTTTCCT TGAGCAGTTC AGAAGAAGAG
    TTGGCGTCTA CGCCGGAGTA CACGAGCTGC GATGATGTGG AGATTGAAAG TGAAAGTGTT
    AGTGAGAAAG GGGACAGTCA AAGGGGAAAA AGAAAAACTA GTGAGCAGGC AGTTTTGTCG
    GATTCTAACA TCTTGTCTGA GAGACAAAAG AAAATGGTGT GCTTTGGAGG CCACTCTTTG
    GAAGAGGATT TGGAATGGTC TGAGCCTCAG ATTAAAGACT CTGGGGTAGA CACGTGTAGT
    AGCACCACCC TTAACGAAGA GCATAGCCAT AGCGAGAAGC ACCCTGTCAC GTGGCAACCT
    TCCAAAGATG GAGACCGCTT AATTGGTCGC ATATTATTAA ACAAGCGGCT CAAAGATGGG
    AGCGTCCCGC GAGATTCTGG AGCCATGCTG GGGCTGAAGG TCGTTGGAGG GAAAATGACA
    GAATCAGGTC GACTTTGTGC GTTTATCACC AAAGTGAAGA AGGGAAGCCT GGCAGATACA
    GTAGGCCATC TTCGACCAGG TGATGAGGTT TTAGAATGGA GTGGGCGGAT TTTGCAAGGA
    GCGACGTTCG AAGAAGTTTA CAATATTATA CTGGAGTCCA AACCCGAGGC ACAAGTAGAA
    TTATTGGTCT CCAGACCCAT AGGAGATATA CCCCGGATTC CTGACAGCAC ACACGCACAA
    CTGGAATCCA GTTCTAGCTC ATTTGAATCC CAGAAAATGG ATCGCCCTTC TATTTCAGTT
    ACTTCCCCCA TGAGTCCTGG AATGTTACGG GATGTCCCCC AGTTTCTTTC TGGACAACTT
    TCAAGCCAAA GCCTTAATAG AAGAACAGCG CCTTTTGTTC CTAGGGTTCA GATTAAACTC
    TGGTATGACA AGGTTGGCCA CCAGTTAATT GTCACGATAT TAGGAGCAAA GGATCTGCCA
    TCGCGGGAAG ATGGGAGGCC GAGAAATCCT TACGTAAAAA TTTACTTTCT TCCAGATCGA
    AGTGATAAGA ACAAAAGGAG AACAAAGACT GTGAAGAAAA CCGTAGAGCC CAAATGGAAC
    CAAACATTCA TCTATTCTCC GGTTCATCGG AGGGAATTTC GGGAACGAAT GTTGGAAATA
    ACACTTTGGG ATCAAGCTCG GGTTCGAGAA GAAGAGAGTG AATTTTTAGG AGAGATTCTA
    ATTGAATTAG AAACTGCACT ATTAGATGAT GAGCCACATT GGTACAAGCT ACAGACGCAT
    GATGTGTCTT CATTGCCACT TCCACACCCA TCCCCGTTCA TGCAAAGAAG GCAGCTGCAC
    GGCGAGAGCC CAACCCGGAG GTTGCAAAAT AAAGGCTCAT ATTCATATAA TTCAGGATCC
    AAAAGGATAA GCGACAGCGA AGTGTCTGAC TACGACTGTG ATGATGGCGT TGGTGTTGTT
    TCAGACTTTC GGCATAATGG GCGTGACCTG CAGAGTTCAA CGTTATCTGT TCCGGAACAA
    GTAATGTCGT CCAATCACTG TTCCCGGACT GGGCCCCCGC ATGTAGACAG CATAGGAAGA
    ACGAGGTCAT GGTCTCCCAG TGTCCCTCCT CCGCAAAGTA GGAATTTGGA ACAGGGACCG
    AGAGGGACAA GATCTGGCCC TGCACATTAC AATACCATGA GCAGAGTGGA AAGGCATCGA
    GTATTAGATA ACCACTACTC CCCAGACAGA GACAGGAATT GTGAAGCGGC AGATAGACAG
    CCATATCAAA GATCCAGGTC CACTGAACAA CGGCCTGTGC TGGAGCGGAC CAGCTCCCGC
    TCCCGATCTA CTGAGCGTCC TGACTCAAAC CTCATGAGGT CGATGCCTTC ATTAATGACG
    GGAAGATCTG CCCCTCCTTC ACCCGCCTTA TCGAGGGCAC ACCCTCCTCG TACCGGCTCT
    GTTCAGACAA GTCCTTCAGG CACCCCGTTA GCAGGGCGGA GGGGGCGCCA GCTCCCGCAA
    CTTCCCCCAA AAGGCACGTT AGAAAGAAAA GTTACTTGGG AAGACCAGCA AAGAAAGGTG
    AATGGTTCAA TAACTGATGA CAAACATTTG CCAAGAAAGA AACAGCATTC TGAGCCAGAA
    GATATGGACT CAACGCGGAG GCGAAACTCA GAGGAAAAGG CTGCAGAACA AGAGAATGGG
    AACCCAGGTG CTATGGACGT AGAAGAAAGG ACGCGACAGC TGAAAGTGAA GATCAACAAA
    TACAAGCAGG TGGGAGGATC AGATTCTCGG CTAGAGAAGG ATTATCATCC AAAGTATCGG
    ACGGGGCGGG ATCCGCAGAG GGGCTCAGAC AATATGTCCA ACAAGTCATC AGACAGCGAT
    GTTAGTGATG TGTCTGCCGT ATCACGGACC AGCAGCGCCT CTCGTTTTAG TAGTACGAGT
    TATATGTCTG TGCAGTCGGA AAGGCCTCGA GGGAACATGA AAATAGGTAG AAGGACAAGG
    GAAGTAGAGG AGAGAATAGA GAGGGCTGAG GGAGGGCAGC ATCACAAAGA AATAGGTTTT
    CTGGAGGAAG AGGAGGACGT TAGGCAGGAA GTAGATAGTG AGAAGGAAAC AGAAGTGCAG
    CATATAATTG ACAACTGCAT GAAGGGGCCA AGCAAAGAAA GTGTAGAGGA AGAGAGTCAA
    CAGAATGAGA AAGATGAGGA AAGCCATGAT GTTGAGGAAG CCCATAATGA GAATCTGCCA
    AGGAGGTCCT CAGAAAATGA TATAAGTGCA TTGGCATCCA AAATGCAAAG CAGGCAAATG
    GGCGCCTCAG GGAAGAACAT GACCAAAAGT ACCAGCATCG GAGGGGATAT GTACACCCTG
    GAGAAGAACG ACGGAAGCCA GTCTGACACA GCCGTGGGCA CTGTGGGTGC GGGTGGAAAG
    AAGAGACGTT CAAGCATAGG CGCCAAAATG GTGGCCATTG TGGGCTTGTC ACGAAAAAGC
    CGAAGTACCT CACAGCTCAG CCAAACAGAA GTCGGAGGGA AGAAGCTGAA GAGCACCATC
    CAGAGAAGTA CGGAAACAGG CCTGGCCGTG GAAATGAGAA ACTGGATGAG CCGCCAAGCA
    AGCAGGGAGT CCACAGATGG GAGTATGAAC AGCTACAGCT CAGAGGGGAA TCTGATATTC
    CCTGGGGTCC GACTGGGTTC CGACAGCCAG TTCAGTGACT TTTTGGATGG TCTTGGTCCT
    GCCCAGCTGG TTGGTCGCCA GACTTTGGCC ACGCCCTCTA TGGGAGATAT TCAGGTTGGA
    ATGGCTGACA AAAAAGGCCA GTTGGAGGTG GAGGTGATAC GAGCCCGTGG TCTTGTTGTA
    AAACCAGGCT CAAAGACACT GCCAGCACCC TATGTTAAGG TGTATCTCTT AGAAAATGGA
    GTGTGCATAG CCAAAAAGAA AACAAAAGTA GCAAGAAAAA CGCTGGAGCC TCTATACCAG
    CAAGTGTTAG CGTTTGAGGA ATCACCACAA GGGAAGGTCC TACAGATCAT TGTTTGGGGT
    GACTATGGAC GCATGGACCA CAAGTCCTTC ATGGGGGTGG CTCAGATACT TTTAGATGAA
    CTGGACCTAT CCAGCATGGT GATTGGATGG TATAAACTTT TCCCCCCTTC ATCTTTAGTA
    GACCCAACGT TGGCTCCACT CACTAGGAGA GCTTCCCAAT CATCTCTTGA AAGCTCCACG
    GGACCTTCTT ATGCTCGTTC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031759435.1
    CDS275..4798
    Translation

    Target ORF information:

    RefSeq Version XM_031903575.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 2 (rims2), transcript variant X29, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031903575.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCATTCGC ATGAAAATGA TGCCGGCTCT TTTCCAGATG TGCAATGGCT GTTTGCAAGG 
    AAAAGAAGCC CTTCAATACC TAGAGACCAG AATCGGAGAT ATGATACAAG GGACGATATA
    GGTGAACATT CACAGCATGC TACATCAGAC AGCGCCATGC CTAGATCACC ATCAGACTAC
    TCGGACAGGC GATTGCAACG AGGGTCTCAG CTCTATGATG AGCCTGAGCA CGGTGACTAC
    AGAAGAAGTA GGCGGCGCTC CAATGAGTAT TCGGTAGATG AGGAGGATAT CCAAGACAGG
    GAGGAATATG AAAGGCAGAG AAGAGAGGAG GAGTACCAGG CAAGGTACCG GAGTGACCCC
    AACTTGGCCA GGTACCCTGT TAAGCCACAA CCGTATGAAG AGCAAATGCG CATCCATGCA
    GAGGTTTCAA GGGCTCGACA ACGAAGGCAC AGCGATGTGT CTTTAGCTCA GACAGAATTT
    GAGGAGTCTC GGATGTCAAC GCTGAGGATG GAGAGACCTT CAAGGCAAAG ATCTGCGTCA
    GAGCGTAGAG CAGCGATGGA AAGCCAGCGT TCATATTCAA TAGAAAGAAC TAGAGATGTG
    CAAGGGCCAG GTCCTGGTCG GCAAAGGACC TCAAATCATA GTCCCCCAAC ACCAAGAAGG
    AGTCCCATTC CCAATGATAG GCCAGATATG AGGCGCACAG ATTCTTTACG GAAACAGCAC
    CACTTGGACC CAAGTTCTGC CGTAAGGAAA ACCAAAAGGG AAAAAATGGA AACTATGTTA
    AGGAATGATT CTCTTAGCTC AGATCAGTCT GAATCTGTCC GGCCCCCACC ACCAAAGCCT
    CATAAAACAA AAAAGGGAGG TAAAATGCGT CAGGTTTCCT TGAGCAGTTC AGAAGAAGAG
    TTGGCGTCTA CGCCGGAGTA CACGAGCTGC GATGATGTGG AGATTGAAAG TGAAAGTGTT
    AGTGAGAAAG GGGACAGTCA AAGGGGAAAA AGAAAAACTA GTGAGCAGGC AGTTTTGTCG
    GATTCTAACA TCTTGTCTGA GAGACAAAAG AAAATGGTGT GCTTTGGAGG CCACTCTTTG
    GAAGAGGATT TGGAATGGTC TGAGCCTCAG ATTAAAGACT CTGGGGTAGA CACGTGTAGT
    AGCACCACCC TTAACGAAGA GCATAGCCAT AGCGAGAAGC ACCCTGTCAC GTGGCAACCT
    TCCAAAGATG GAGACCGCTT AATTGGTCGC ATATTATTAA ACAAGCGGCT CAAAGATGGG
    AGCGTCCCGC GAGATTCTGG AGCCATGCTG GGGCTGAAGG TCGTTGGAGG GAAAATGACA
    GAATCAGGTC GACTTTGTGC GTTTATCACC AAAGTGAAGA AGGGAAGCCT GGCAGATACA
    GTAGGCCATC TTCGACCAGG TGATGAGGTT TTAGAATGGA GTGGGCGGAT TTTGCAAGGA
    GCGACGTTCG AAGAAGTTTA CAATATTATA CTGGAGTCCA AACCCGAGGC ACAAGTAGAA
    TTATTGGTCT CCAGACCCAT AGGAGATATA CCCCGGATTC CTGACAGCAC ACACGCACAA
    CTGGAATCCA GTTCTAGCTC ATTTGAATCC CAGAAAATGG ATCGCCCTTC TATTTCAGTT
    ACTTCCCCCA TGAGTCCTGG AATGTTACGG GATGTCCCCC AGTTTCTTTC TGGACAACTT
    TCAAGCCAAA GCCTTAATAG AAGAACAGCG CCTTTTGTTC CTAGGGTTCA GATTAAACTC
    TGGTATGACA AGGTTGGCCA CCAGTTAATT GTCACGATAT TAGGAGCAAA GGATCTGCCA
    TCGCGGGAAG ATGGGAGGCC GAGAAATCCT TACGTAAAAA TTTACTTTCT TCCAGATCGA
    AGTGATAAGA ACAAAAGGAG AACAAAGACT GTGAAGAAAA CCGTAGAGCC CAAATGGAAC
    CAAACATTCA TCTATTCTCC GGTTCATCGG AGGGAATTTC GGGAACGAAT GTTGGAAATA
    ACACTTTGGG ATCAAGCTCG GGTTCGAGAA GAAGAGAGTG AATTTTTAGG AGAGATTCTA
    ATTGAATTAG AAACTGCACT ATTAGATGAT GAGCCACATT GGTACAAGCT ACAGACGCAT
    GATGTGTCTT CATTGCCACT TCCACACCCA TCCCCGTTCA TGCAAAGAAG GCAGCTGCAC
    GGCGAGAGCC CAACCCGGAG GTTGCAAAAT AAAGGCTCAT ATTCATATAA TTCAGGATCC
    AAAAGGATAA GCGACAGCGA AGTGTCTGAC TACGACTGTG ATGATGGCGT TGGTGTTGTT
    TCAGACTTTC GGCATAATGG GCGTGACCTG CAGAGTTCAA CGTTATCTGT TCCGGAACAA
    GTAATGTCGT CCAATCACTG TTCCCGGACT GGGCCCCCGC ATGTAGACAG CATAGGAAGA
    ACGAGGTCAT GGTCTCCCAG TGTCCCTCCT CCGCAAAGTA GGAATTTGGA ACAGGGACCG
    AGAGGGACAA GATCTGGCCC TGCACATTAC AATACCATGA GCAGAGTGGA AAGGCATCGA
    GTATTAGATA ACCACTACTC CCCAGACAGA GACAGGAATT GTGAAGCGGC AGATAGACAG
    CCATATCAAA GATCCAGGTC CACTGAACAA CGGCCTGTGC TGGAGCGGAC CAGCTCCCGC
    TCCCGATCTA CTGAGCGTCC TGACTCAAAC CTCATGAGGT CGATGCCTTC ATTAATGACG
    GGAAGATCTG CCCCTCCTTC ACCCGCCTTA TCGAGGGCAC ACCCTCCTCG TACCGGCTCT
    GTTCAGACAA GTCCTTCAGG CACCCCGTTA GCAGGGCGGA GGGGGCGCCA GCTCCCGCAA
    CTTCCCCCAA AAGGCACGTT AGAAAGAAAA GTTACTTGGG AAGACCAGCA AAGAAAGGTG
    AATGGTTCAA TAACTGATGA CAAACATTTG CCAAGAAAGA AACAGCATTC TGAGCCAGAA
    GATATGGACT CAACGCGGAG GCGAAACTCA GAGGAAAAGG CTGCAGAACA AGAGAATGGG
    AACCCAGGTG CTATGGACGT AGAAGAAAGG ACGCGACAGC TGAAAGTGAA GATCAACAAA
    TACAAGCAGG TGGGAGGATC AGATTCTCGG CTAGAGAAGG ATTATCATCC AAAGTATCGG
    ACGGGGCGGG ATCCGCAGAG GGGCTCAGAC AATATGTCCA ACAAGTCATC AGACAGCGAT
    GTTAGTGATG TGTCTGCCGT ATCACGGACC AGCAGCGCCT CTCGTTTTAG TAGTACGAGT
    TATATGTCTG TGCAGTCGGA AAGGCCTCGA GGGAACATGA AAATAGGTAG AAGGACAAGG
    GAAGTAGAGG AGAGAATAGA GAGGGCTGAG GGAGGGCAGC ATCACAAAGA AATAGGTTTT
    CTGGAGGAAG AGGAGGACGT TAGGCAGGAA GTAGATAGTG AGAAGGAAAC AGAAGTGCAG
    CATATAATTG ACAACTGCAT GAAGGGGCCA AGCAAAGAAA GTGTAGAGGA AGAGAGTCAA
    CAGAATGAGA AAGATGAGGA AAGCCATGAT GTTGAGGAAG CCCATAATGA GAATCTGCCA
    AGGAGGTCCT CAGAAAATGA TATAAGTGCA TTGGCATCCA AAATGCAAAG CAGGCAAATG
    GGCGCCTCAG GGAAGAACAT GACCAAAAGT ACCAGCATCG GAGGGGATAT GTACACCCTG
    GAGAAGAACG ACGGAAGCCA GTCTGACACA GCCGTGGGCA CTGTGGGTGC GGGTGGAAAG
    AAGAGACGTT CAAGCATAGG CGCCAAAATG GTGGCCATTG TGGGCTTGTC ACGAAAAAGC
    CGAAGTACCT CACAGCTCAG CCAAACAGAA GTCGGAGGGA AGAAGCTGAA GAGCACCATC
    CAGAGAAGTA CGGAAACAGG CCTGGCCGTG GAAATGAGAA ACTGGATGAG CCGCCAAGCA
    AGCAGGGAGT CCACAGATGG GAGTATGAAC AGCTACAGCT CAGAGGGGAA TCTGATATTC
    CCTGGGGTCC GACTGGGTTC CGACAGCCAG TTCAGTGACT TTTTGGATGG TCTTGGTCCT
    GCCCAGCTGG TTGGTCGCCA GACTTTGGCC ACGCCCTCTA TGGGAGATAT TCAGGTTGGA
    ATGGCTGACA AAAAAGGCCA GTTGGAGGTG GAGGTGATAC GAGCCCGTGG TCTTGTTGTA
    AAACCAGGCT CAAAGACACT GCCAGCACCC TATGTTAAGG TGTATCTCTT AGAAAATGGA
    GTGTGCATAG CCAAAAAGAA AACAAAAGTA GCAAGAAAAA CGCTGGAGCC TCTATACCAG
    CAAGTGTTAG CGTTTGAGGA ATCACCACAA GGGAAGGTCC TACAGATCAT TGTTTGGGGT
    GACTATGGAC GCATGGACCA CAAGTCCTTC ATGGGGGTGG CTCAGATACT TTTAGATGAA
    CTGGACCTAT CCAGCATGGT GATTGGATGG TATAAACTTT TCCCCCCTTC ATCTTTAGTA
    GACCCAACGT TGGCTCCACT CACTAGGAGA GCTTCCCAAT CATCTCTTGA AAGCTCCACG
    GGACCTTCTT ATGCTCGTTC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa11423
    Clone ID Related Accession (Same CDS sequence) XM_004915096.3
    Accession Version XM_004915096.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4707bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulating synaptic membrane exocytosis protein 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004915096.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTCTGCTC CACTAGGGCC CAGGGGCGGC CCTCTGCCTC CTGCACAGCC ACAGGCTCCA 
    ATGCCAGAGA TGCCGGACCT CAGCCACCTG ACAGAGGATG AGAGGAAAAT CATAATGGCT
    GTGATGGAGA GGCAGAAGAA AGAAGAGGAG AAGGAGCAGT CAGTGTTAAA AAAATTGCAC
    CAGCAGTTTG AAATGTACAA GGAGCAAGTG AAGAAGATGG GCGAGGAAGC ACAGCAGCAG
    CAGGAGCAGA AGGGCGAAGC TCCAACCTGT GGAATCTGCC ACAAAACAAA GTTTGCTGAT
    GGCTGTGGCC ACGACTGTTC GTATTGCCAA ACTAAATTCT GCGCGCGGTG TGGAGGGCGA
    GTGTCTCTGC GCTCAAATAA GGTAATGTGG GTATGTAATT TGTGCCGAAA ACAACAAGAA
    ATCCTCACCA AATCAGGGGC CTGGTTTTAC AGCAGTGGGT CAAGCACACC CCTAAAGCCC
    GGCGAAGAGA GCCAGGCAGG GTACCAAACG GAAGAAGCAC CTCAGGAGAA AAAAGCTAAG
    CTACAAGAGC AGTCCCAATA CCAGGGACCC TCAGGGGACT TATTAACACC AGCCTCGGAC
    AAAAACCGAC CGCAAGGGAT CGTCCGGCAA GATTCCATCA AAAATGGTTC CGAACTGAAG
    CACCCGATTC CTGGCGACCT CGCATCTGAC AGGAAAAGAA GCCCTTCAAT ACCTAGAGAC
    CAGAATCGGA GATATGATAC AAGGGACGAT ATAGGTGAAC ATTCACAGCA TGCTACATCA
    GACAGCGCCA TGCCTAGATC ACCATCAGAC TACTCGGACA GGCGATTGCA ACGAGGGTCT
    CAGCTCTATG ATGAGCCTGA GCACGGTGAC TACAGAAGAA GTAGGCGGCG CTCCAATGAG
    TATTCGGTAG ATGAGGAGGA TATCCAAGAC AGGGAGGAAT ATGAAAGGCA GAGAAGAGAG
    GAGGAGTACC AGGCAAGGTA CCGGAGTGAC CCCAACTTGG CCAGGTACCC TGTTAAGCCA
    CAACCGTATG AAGAGCAAAT GCGCATCCAT GCAGAGGTTT CAAGGGCTCG ACAACGAAGG
    CACAGCGATG TGTCTTTAGC TCAGACAGAA TTTGAGGAGT CTCGGATGTC AACGCTGAGG
    ATGGAGAGAC CTTCAAGGCA AAGATCTGCG TCAGAGCGTA GAGCAGCGAT GGAAAGCCAG
    CGTTCATATT CAATAGAAAG AACTAGAGAT GTGCAAGGGC CAGGTCCTGG TCGGCAAAGG
    ACCTCAAATC ATAGTCCCCC AACACCAAGA AGGAGTCCCA TTCCCAATGA TAGGCCAGAT
    ATGAGGCGCA CAGATTCTTT ACGGAAACAG CACCACTTGG ACCCAAGTTC TGCCGTAAGG
    AAAACCAAAA GGGAAAAAAT GGAAACTATG TTAAGGAATG ATTCTCTTAG CTCAGATCAG
    TCTGAATCTG TCCGGCCCCC ACCACCAAAG CCTCATAAAA CAAAAAAGGG AGGTAAAATG
    CGTCAGGTTT CCTTGAGCAG TTCAGAAGAA GAGTTGGCGT CTACGCCGGA GTACACGAGC
    TGCGATGATG TGGAGATTGA AAGTGAAAGT GTTAGTGAGA AAGGGGACAG TCAAAGGGGA
    AAAAGAAAAA CTAGTGAGCA GGCAGTTTTG TCGGATTCTA ACATCTTGTC TGAGAGACAA
    AAGAAAATGG TGTGCTTTGG AGGCCACTCT TTGGAAGAGG ATTTGGAATG GTCTGAGCCT
    CAGATTAAAG ACTCTGGGGT AGACACGTGT AGTAGCACCA CCCTTAACGA AGAGCATAGC
    CATAGCGAGA AGCACCCTGT CACGTGGCAA CCTTCCAAAG ATGGAGACCG CTTAATTGGT
    CGCATATTAT TAAACAAGCG GCTCAAAGAT GGGAGCGTCC CGCGAGATTC TGGAGCCATG
    CTGGGGCTGA AGGTCGTTGG AGGGAAAATG ACAGAATCAG GTCGACTTTG TGCGTTTATC
    ACCAAAGTGA AGAAGGGAAG CCTGGCAGAT ACAGTAGGCC ATCTTCGACC AGGTGATGAG
    GTTTTAGAAT GGAGTGGGCG GATTTTGCAA GGAGCGACGT TCGAAGAAGT TTACAATATT
    ATACTGGAGT CCAAACCCGA GGCACAAGTA GAATTATTGG TCTCCAGACC CATAGGAGAT
    ATACCCCGGA TTCCTGACAG CACACACGCA CAACTGGAAT CCAGTTCTAG CTCATTTGAA
    TCCCAGAAAA TGGATCGCCC TTCTATTTCA GTTACTTCCC CCATGAGTCC TGGAATGTTA
    CGGGATGTCC CCCAGTTTCT TTCTGGACAA CTTTCAATTA AACTCTGGTA TGACAAGGTT
    GGCCACCAGT TAATTGTCAC GATATTAGGA GCAAAGGATC TGCCATCGCG GGAAGATGGG
    AGGCCGAGAA ATCCTTACGT AAAAATTTAC TTTCTTCCAG ATCGAAGTGA TAAGAACAAA
    AGGAGAACAA AGACTGTGAA GAAAACCGTA GAGCCCAAAT GGAACCAAAC ATTCATCTAT
    TCTCCGGTTC ATCGGAGGGA ATTTCGGGAA CGAATGTTGG AAATAACACT TTGGGATCAA
    GCTCGGGTTC GAGAAGAAGA GAGTGAATTT TTAGGAGAGA TTCTAATTGA ATTAGAAACT
    GCACTATTAG ATGATGAGCC ACATTGGTAC AAGCTACAGA CGCATGATGT GTCTTCATTG
    CCACTTCCAC ACCCATCCCC GTTCATGCAA AGAAGGCAGC TGCACGGCGA GAGCCCAACC
    CGGAGGTTGC AAAGATCCAA AAGGATAAGC GACAGCGAAG TGTCTGACTA CGACTGTGAT
    GATGGCGTTG GTGTTGTTTC AGACTTTCGG CATAATGGGC GTGACCTGCA GAGTTCAACG
    TTATCTGTTC CGGAACAAGT AATGTCGTCC AATCACTGTT CCCGGACTGG GCCCCCGCAT
    GTAGACAGCA TAGGAAGAAC GAGGTCATGG TCTCCCAGTG TCCCTCCTCC GCAAAGGAAT
    TTGGAACAGG GACCGAGAGG GACAAGATCT GGCCCTGCAC ATTACAATAC CATGAGCAGA
    GTGGAAAGGC ATCGAGTATT AGATAACCAC TACTCCCCAG ACAGAGACAG TCATTATCTT
    ACTCTACCTC GCACCAGATT CAGTCAGTCC TTTGACCATC ACCTAGCAGA GCCAAGGAAT
    TGTGAAGCGG CAGATAGACA GCCATATCAA AGATCCAGGT CCACTGAACA ACGGCCTGTG
    CTGGAGCGGA CCAGCTCCCG CTCCCGATCT ACTGAGCGTC CTGACTCAAA CCTCATGAGG
    TCGATGCCTT CATTAATGAC GGGAAGATCT GCCCCTCCTT CACCCGCCTT ATCGAGGGCA
    CACCCTCCTC GTACCGGCTC TGTTCAGACA AGTCCTTCAG GCACCCCGTT AGCAGGGCGG
    AGGGGGCGCC AGCTCCCGCA ACTTCCCCCA AAAGGCACGT TAGAAAGAAG TGCTATGGAC
    GTAGAAGAAA GAACGCGACA GCTGAAAGTG AAGATCAACA AATACAAGCA GGTGGGAGGA
    TCAGATTCTC GGCTAGAGAA GGATTATCAT CCAAAGTATC GGACGGGGCG GGATCCGCAG
    AGGGGCTCAG ACAATATGTC CAACAAGTCA TCAGACAGCG ATGTTAGTGA TGTGTCTGCC
    GTATCACGGA CCAGCAGCGC CTCTCGTTTT AGTAGTACGA GTTATATGTC TGTGCAGTCG
    GAAAGGCCTC GAGGGAACAT GAAAATAGGT GCATTGGCAT CCAAAATGCA AAGCAGGCAA
    ATGGGCGCCT CAGGGAAGAA CATGACCAAA AGTACCAGCA TCGGAGGGGA TATGTACACC
    CTGGAGAAGA ACGACGGAAG CCAGTCTGAC ACAGCCGTGG GCACTGTGGG TGCGGGTGGA
    AAGAAGAGAC GTTCAAGCAT AGGCGCCAAA ATGGTGGCCA TTGTGGGCTT GTCACGAAAA
    AGCCGAAGTA CCTCACAGCT CAGCCAAACA GAAGTCGGAG GGAAGAAGCT GAAGAGCACC
    ATCCAGAGAA GTACGGAAAC AGGCCTGGCC GTGGAAATGA GAAACTGGAT GAGCCGCCAA
    GCAAGCAGGG AGTCCACAGA TGGGAGTATG AACAGCTACA GCTCAGAGGG GAATCTGATA
    TTCCCTGGGG TCCGACTGGG TTCCGACAGC CAGTTCAGTG ACTTTTTGGA TGGTCTTGGT
    CCTGCCCAGC TGGTTGGTCG CCAGACTTTG GCCACGCCCT CTATGGGAGA TATTCAGGTT
    GGAATGGCTG ACAAAAAAGG CCAGTTGGAG GTGGAGGTGA TACGAGCCCG GGGTCTTGTT
    GTAAAACCAG GCTCAAAGAC ACTGCCAGCA CCCTATGTTA AGGTGTATCT CTTAGAAAAT
    GGAGTGTGCA TAGCCAAAAA GAAAACAAAA GTAGCAAGAA AAACGCTGGA GCCTCTATAC
    CAGCAAGTGT TAGCGTTTGA GGAATCACCA CAAGGGAAGG TCCTACAGAT CATTGTTTGG
    GGTGACTATG GACGCATGGA CCACAAGTCC TTCATGGGGG TGGCTCAGAT ACTTTTAGAT
    GAACTGGACC TATCCAGCAT GGTGATTGGA TGGTATAAAC TTTTCCCCCC TTCATCTTTA
    GTAGACCCAA CGTTGGCTCC ACTCACTAGG AGAGCTTCCC AATCATCTCT TGAAAGCTCC
    ACGGGACCTT CTTATGCTCG TTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004915153.1
    CDS648..5354
    Translation

    Target ORF information:

    RefSeq Version XM_004915096.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulating synaptic membrane exocytosis 2 (rims2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004915096.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTCTGCTC CACTAGGGCC CAGGGGCGGC CCTCTGCCTC CTGCACAGCC ACAGGCTCCA 
    ATGCCAGAGA TGCCGGACCT CAGCCACCTG ACAGAGGATG AGAGGAAAAT CATAATGGCT
    GTGATGGAGA GGCAGAAGAA AGAAGAGGAG AAGGAGCAGT CAGTGTTAAA AAAATTGCAC
    CAGCAGTTTG AAATGTACAA GGAGCAAGTG AAGAAGATGG GCGAGGAAGC ACAGCAGCAG
    CAGGAGCAGA AGGGCGAAGC TCCAACCTGT GGAATCTGCC ACAAAACAAA GTTTGCTGAT
    GGCTGTGGCC ACGACTGTTC GTATTGCCAA ACTAAATTCT GCGCGCGGTG TGGAGGGCGA
    GTGTCTCTGC GCTCAAATAA GGTAATGTGG GTATGTAATT TGTGCCGAAA ACAACAAGAA
    ATCCTCACCA AATCAGGGGC CTGGTTTTAC AGCAGTGGGT CAAGCACACC CCTAAAGCCC
    GGCGAAGAGA GCCAGGCAGG GTACCAAACG GAAGAAGCAC CTCAGGAGAA AAAAGCTAAG
    CTACAAGAGC AGTCCCAATA CCAGGGACCC TCAGGGGACT TATTAACACC AGCCTCGGAC
    AAAAACCGAC CGCAAGGGAT CGTCCGGCAA GATTCCATCA AAAATGGTTC CGAACTGAAG
    CACCCGATTC CTGGCGACCT CGCATCTGAC AGGAAAAGAA GCCCTTCAAT ACCTAGAGAC
    CAGAATCGGA GATATGATAC AAGGGACGAT ATAGGTGAAC ATTCACAGCA TGCTACATCA
    GACAGCGCCA TGCCTAGATC ACCATCAGAC TACTCGGACA GGCGATTGCA ACGAGGGTCT
    CAGCTCTATG ATGAGCCTGA GCACGGTGAC TACAGAAGAA GTAGGCGGCG CTCCAATGAG
    TATTCGGTAG ATGAGGAGGA TATCCAAGAC AGGGAGGAAT ATGAAAGGCA GAGAAGAGAG
    GAGGAGTACC AGGCAAGGTA CCGGAGTGAC CCCAACTTGG CCAGGTACCC TGTTAAGCCA
    CAACCGTATG AAGAGCAAAT GCGCATCCAT GCAGAGGTTT CAAGGGCTCG ACAACGAAGG
    CACAGCGATG TGTCTTTAGC TCAGACAGAA TTTGAGGAGT CTCGGATGTC AACGCTGAGG
    ATGGAGAGAC CTTCAAGGCA AAGATCTGCG TCAGAGCGTA GAGCAGCGAT GGAAAGCCAG
    CGTTCATATT CAATAGAAAG AACTAGAGAT GTGCAAGGGC CAGGTCCTGG TCGGCAAAGG
    ACCTCAAATC ATAGTCCCCC AACACCAAGA AGGAGTCCCA TTCCCAATGA TAGGCCAGAT
    ATGAGGCGCA CAGATTCTTT ACGGAAACAG CACCACTTGG ACCCAAGTTC TGCCGTAAGG
    AAAACCAAAA GGGAAAAAAT GGAAACTATG TTAAGGAATG ATTCTCTTAG CTCAGATCAG
    TCTGAATCTG TCCGGCCCCC ACCACCAAAG CCTCATAAAA CAAAAAAGGG AGGTAAAATG
    CGTCAGGTTT CCTTGAGCAG TTCAGAAGAA GAGTTGGCGT CTACGCCGGA GTACACGAGC
    TGCGATGATG TGGAGATTGA AAGTGAAAGT GTTAGTGAGA AAGGGGACAG TCAAAGGGGA
    AAAAGAAAAA CTAGTGAGCA GGCAGTTTTG TCGGATTCTA ACATCTTGTC TGAGAGACAA
    AAGAAAATGG TGTGCTTTGG AGGCCACTCT TTGGAAGAGG ATTTGGAATG GTCTGAGCCT
    CAGATTAAAG ACTCTGGGGT AGACACGTGT AGTAGCACCA CCCTTAACGA AGAGCATAGC
    CATAGCGAGA AGCACCCTGT CACGTGGCAA CCTTCCAAAG ATGGAGACCG CTTAATTGGT
    CGCATATTAT TAAACAAGCG GCTCAAAGAT GGGAGCGTCC CGCGAGATTC TGGAGCCATG
    CTGGGGCTGA AGGTCGTTGG AGGGAAAATG ACAGAATCAG GTCGACTTTG TGCGTTTATC
    ACCAAAGTGA AGAAGGGAAG CCTGGCAGAT ACAGTAGGCC ATCTTCGACC AGGTGATGAG
    GTTTTAGAAT GGAGTGGGCG GATTTTGCAA GGAGCGACGT TCGAAGAAGT TTACAATATT
    ATACTGGAGT CCAAACCCGA GGCACAAGTA GAATTATTGG TCTCCAGACC CATAGGAGAT
    ATACCCCGGA TTCCTGACAG CACACACGCA CAACTGGAAT CCAGTTCTAG CTCATTTGAA
    TCCCAGAAAA TGGATCGCCC TTCTATTTCA GTTACTTCCC CCATGAGTCC TGGAATGTTA
    CGGGATGTCC CCCAGTTTCT TTCTGGACAA CTTTCAATTA AACTCTGGTA TGACAAGGTT
    GGCCACCAGT TAATTGTCAC GATATTAGGA GCAAAGGATC TGCCATCGCG GGAAGATGGG
    AGGCCGAGAA ATCCTTACGT AAAAATTTAC TTTCTTCCAG ATCGAAGTGA TAAGAACAAA
    AGGAGAACAA AGACTGTGAA GAAAACCGTA GAGCCCAAAT GGAACCAAAC ATTCATCTAT
    TCTCCGGTTC ATCGGAGGGA ATTTCGGGAA CGAATGTTGG AAATAACACT TTGGGATCAA
    GCTCGGGTTC GAGAAGAAGA GAGTGAATTT TTAGGAGAGA TTCTAATTGA ATTAGAAACT
    GCACTATTAG ATGATGAGCC ACATTGGTAC AAGCTACAGA CGCATGATGT GTCTTCATTG
    CCACTTCCAC ACCCATCCCC GTTCATGCAA AGAAGGCAGC TGCACGGCGA GAGCCCAACC
    CGGAGGTTGC AAAGATCCAA AAGGATAAGC GACAGCGAAG TGTCTGACTA CGACTGTGAT
    GATGGCGTTG GTGTTGTTTC AGACTTTCGG CATAATGGGC GTGACCTGCA GAGTTCAACG
    TTATCTGTTC CGGAACAAGT AATGTCGTCC AATCACTGTT CCCGGACTGG GCCCCCGCAT
    GTAGACAGCA TAGGAAGAAC GAGGTCATGG TCTCCCAGTG TCCCTCCTCC GCAAAGGAAT
    TTGGAACAGG GACCGAGAGG GACAAGATCT GGCCCTGCAC ATTACAATAC CATGAGCAGA
    GTGGAAAGGC ATCGAGTATT AGATAACCAC TACTCCCCAG ACAGAGACAG TCATTATCTT
    ACTCTACCTC GCACCAGATT CAGTCAGTCC TTTGACCATC ACCTAGCAGA GCCAAGGAAT
    TGTGAAGCGG CAGATAGACA GCCATATCAA AGATCCAGGT CCACTGAACA ACGGCCTGTG
    CTGGAGCGGA CCAGCTCCCG CTCCCGATCT ACTGAGCGTC CTGACTCAAA CCTCATGAGG
    TCGATGCCTT CATTAATGAC GGGAAGATCT GCCCCTCCTT CACCCGCCTT ATCGAGGGCA
    CACCCTCCTC GTACCGGCTC TGTTCAGACA AGTCCTTCAG GCACCCCGTT AGCAGGGCGG
    AGGGGGCGCC AGCTCCCGCA ACTTCCCCCA AAAGGCACGT TAGAAAGAAG TGCTATGGAC
    GTAGAAGAAA GAACGCGACA GCTGAAAGTG AAGATCAACA AATACAAGCA GGTGGGAGGA
    TCAGATTCTC GGCTAGAGAA GGATTATCAT CCAAAGTATC GGACGGGGCG GGATCCGCAG
    AGGGGCTCAG ACAATATGTC CAACAAGTCA TCAGACAGCG ATGTTAGTGA TGTGTCTGCC
    GTATCACGGA CCAGCAGCGC CTCTCGTTTT AGTAGTACGA GTTATATGTC TGTGCAGTCG
    GAAAGGCCTC GAGGGAACAT GAAAATAGGT GCATTGGCAT CCAAAATGCA AAGCAGGCAA
    ATGGGCGCCT CAGGGAAGAA CATGACCAAA AGTACCAGCA TCGGAGGGGA TATGTACACC
    CTGGAGAAGA ACGACGGAAG CCAGTCTGAC ACAGCCGTGG GCACTGTGGG TGCGGGTGGA
    AAGAAGAGAC GTTCAAGCAT AGGCGCCAAA ATGGTGGCCA TTGTGGGCTT GTCACGAAAA
    AGCCGAAGTA CCTCACAGCT CAGCCAAACA GAAGTCGGAG GGAAGAAGCT GAAGAGCACC
    ATCCAGAGAA GTACGGAAAC AGGCCTGGCC GTGGAAATGA GAAACTGGAT GAGCCGCCAA
    GCAAGCAGGG AGTCCACAGA TGGGAGTATG AACAGCTACA GCTCAGAGGG GAATCTGATA
    TTCCCTGGGG TCCGACTGGG TTCCGACAGC CAGTTCAGTG ACTTTTTGGA TGGTCTTGGT
    CCTGCCCAGC TGGTTGGTCG CCAGACTTTG GCCACGCCCT CTATGGGAGA TATTCAGGTT
    GGAATGGCTG ACAAAAAAGG CCAGTTGGAG GTGGAGGTGA TACGAGCCCG GGGTCTTGTT
    GTAAAACCAG GCTCAAAGAC ACTGCCAGCA CCCTATGTTA AGGTGTATCT CTTAGAAAAT
    GGAGTGTGCA TAGCCAAAAA GAAAACAAAA GTAGCAAGAA AAACGCTGGA GCCTCTATAC
    CAGCAAGTGT TAGCGTTTGA GGAATCACCA CAAGGGAAGG TCCTACAGAT CATTGTTTGG
    GGTGACTATG GACGCATGGA CCACAAGTCC TTCATGGGGG TGGCTCAGAT ACTTTTAGAT
    GAACTGGACC TATCCAGCAT GGTGATTGGA TGGTATAAAC TTTTCCCCCC TTCATCTTTA
    GTAGACCCAA CGTTGGCTCC ACTCACTAGG AGAGCTTCCC AATCATCTCT TGAAAGCTCC
    ACGGGACCTT CTTATGCTCG TTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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