bub1b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following bub1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bub1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa64464 XM_031891202.1
Latest version!
Xenopus tropicalis BUB1B, mitotic checkpoint serine/threonine kinase (bub1b), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa30692 XM_012968790.2
Latest version!
Xenopus tropicalis BUB1B, mitotic checkpoint serine/threonine kinase (bub1b), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa64464
    Clone ID Related Accession (Same CDS sequence) XM_031891202.1
    Accession Version XM_031891202.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3135bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mitotic checkpoint serine/threonine-protein kinase BUB1 beta
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCGCAAG CTGGCGATGA ATGGGAGCTA AGCAAAGAAA ATGTGCAACC CCTGCGACAG 
    GGCAGAGTTA TGTCCACTCT GCAGGAGGTG CTCTCACAGC AGGAGAGTGC AAGCCACACT
    GCCATTCAGC AGCAGAAGCA AGCATTTGAG CTGGAACTGC GCTTCTATTC AGGTGATGAT
    CCACTAGATG TCTGGGACAG GTACATCAAG TGGGCTGAGC AGGCATTCCC TCAAGGAGGG
    AAAGAGAGCA ACTTGTGCCC ATTGCTGGAA AGAGCTGTTA AGATATTCCA TGAAGAACAG
    CGATATTATG ATGATCTGCG CTATCTTAGC ATTTGTCTCA AGTTTGCTAA TTTCTGCTCA
    GAGCCTCTGG ATTTGTACAG CTATTTGCAC AGCCAAAGAA TTGGGGTCTC TCATGCCCTA
    CTTTATATCA CTTGGGCCGA ACAGTTTGAA GCTCGAGGTA ATTTCAAGAA AGCTGACTCC
    ATATTTCAGC AGGGCATGCA ACACAAGGCT GAACCACTGG AAAAGCTAGA GATCCATCAT
    CGGCAGTTTC AGGCCCGAGT ATCCCGGCAA ATATTACAAG GAATTTCAGA GGGGCCAGAG
    GCAGAAGAAC CTGAAGTTCC AGAGCCACAG CGCAGCTCAC TGGCAGATCT AAAGTCCAGA
    GGGAAGAACA AAGTGAAAGC ACCCATTAAT CGAGTTGGAG ACTCGATCAA ACCACGTCCT
    CATGGCCTCA GCGTTCAAGC TGCTCCAACA CAGCAAATTC CAAGCCGCTC TCGCTTTGCT
    GTGTTTGATG AAAATGCAGC CTCAGCAGCC CCAGAGCTGC CCTCCTTGAC TCCACAGCAG
    TGGACTGCAC CACCCCCTGC ACGGTCTAAG GAAAATGAAC AGAAAGCTGG ACAATGGAAC
    TCTGGCAGGC CTTCTAGAAA CAGCCATCAG GCTGCTGCTG CCTCTGAACC GCCCCAAACT
    TTGCCCAGTT TCATGCCTTA TGTGGATGAA AGTGCTCAGC AGCAAACAGT AACTCCATGT
    AAAATAAACC CAGCAGTCAC TAGTGTCTTA AGCTCCCGCA AGCCAGGAAA AGAAGAGGAT
    CCTCTGCAAA GGGTACAGAA CAATTCCCAA GGAAAAGAGG AGAAGGTTAT GTACTGCAAG
    GATAAAGTTT ACGCTGGCGT GGAAGAGTTC TCCTTGGAAG AGATAAGGGC AGAAATCTAC
    TTGGCAAAAA TACGGAAGAA GAGAGAAGAC GACCTACAGG AGATTGCACT GAGGAGGCAG
    GAAATGGAGC GACAGATACA GGAGATGGAG AAGCAGCTAA AGGGATCATG TATTGAAAGC
    GAAGAGATCG TAAGTGATCA GCCTGTACAG AATGTTGAAC CTATTATTGC CCCATGTAAT
    TCCAATACAA ATGAGAGTCT GTGTGCATCT CAGCAGGCAC CAGTCATGGA ATTTCCACAT
    TGCTTTGAAA TGGCTGATGC TGCTCCAACA CTGCCAAGGA TGGAGACCCT GCCCGTATTT
    AATGAAACCG GGAACAAGCC CAGCTTGCCA GGTTTGTCTC CAGCACTTTC CTGTGACTTG
    CCCTTCACAA TATTTGATGA ATCTGCAGAA GCTGCACCTA GCATGACTGA CCAGAAGAGA
    ATGGCTGCTC CTGTACGTCG CCCACTTGCT GCTGTTCCTA AGTCCATTGT TAAATCACAA
    GAATCCCAAC TAACCGACAC CCTGGATGGA ACTGAACCCT TAAATGAAGA GGCAATTGTG
    TGTGGCTCAG GTAAAAACAA ATCCTTATTC CCTGACCCAG AAGACACTTG TGATTTTGTG
    AGAGCTGCCC ATCTGGCGTC AACACCTTTC CATAGGGCTA GGGACCAATC AGGCGAATGC
    TTACAGAGTG ACTCGCTTGA TCTGGAAAGG CTCCCGCTTC GAGAGAAGAC ACCTGTGTGC
    GAGGAGTCTT ATAGACAAGA ACTCTGCATA AAGAAACTAA GCCCCATTTT AGAAGCAAGC
    CAAGAAGATA CACGCACATC AGTCTCCTCT GTGTCCTCAA TCTCGAGCAC AACCAGCATG
    TTCACTTCCA GGCCAATTCC AGAGAAGCTC GAGCTGGCTA CTCAGATTAC AGGGGTGTAT
    GAGTCTGAGC CAACCACAGA AGAGCTTCCA CAAGCAGAAG AACTTGCAGA ACTGCATAGG
    CAGTTGCTGG AATCTCTGCC AGAGCTGCTG GTCTCTCCAG AGATCCAACA TGAGGTTGGA
    ACGATGCCTG ATATGAAAGA ACAAGGAGAG GTGGTGCTGG GTTGTGAAAC TTACGGTCTA
    AAAAATGAAG TCATCCTTCA TCCTAACAGC AAGTTGTTTA TGGGTGCCCC TGTGGATTGG
    GATATGGGAG ACATGAAGGC ATTTGCTCTA AAGGTGGATT ATCAGCCACT TCCATGGGAT
    TTCTATGTTA CAATGCAGCT GAAGGAGCGC CTTGGAGATC TATTTGAAAC ATTTTTCATT
    GAACAAACTA ACTGCTTCCT CTACCAGAAT GGGTGCATCA GTCTCTACAA GGATATCAAC
    CGTTTCAGTA TTCAAGAGAT CCTTTTGGAT TCTGAAGAAC ATATCAAAGA AGTAATTGTG
    TTGGTAACAT ACAACTTGTT GAGCCTGGTG GAAAAGCTTC ACTCAGTGGA AATTGTTCAT
    GGAGATCTGA GGCCAGAGAC TCTACTTCTG GATGACAAGA TTTTTGATTT GTCGTCATCT
    CTGGAACTGA AGGGTTTATT TAAGCTGGTA GATTTTTCCC ATAGCGTGGA TCTGAAATTA
    TACCCCATAA TGAGCTCATT ACGTGGCTTC CCCATTGCTC AAACTGAAAG TGGTCAACAA
    TTTCTCAGTC CTCAGTCCTC CCCTTATCAG GTGGACCTTC TTGGCATTGC AGACCTGGTT
    CACCTAATGA TTTTCAGGAA ACCATTGCAG CTGCACCAGG AAAATTCTGT TTGGATGATT
    TGTAAAGAGG TTCCTAGACT GCGTGGTGGA AATCTCTGGA ACAAATTCTT TACAAAGATC
    CTAAATGCAG AAGGCCCAAG CACACGTGTT CTGGGGGAGC TAAAGGGAGA AATGATGGGA
    CAATTTGACA GCTGCTTTCA AGACAAACTT TGCAACTATT TCATCCAGCT GGAGATGAGA
    CTGAACCCGT TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031747062.1
    CDS150..3284
    Translation

    Target ORF information:

    RefSeq Version XM_031891202.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis BUB1B, mitotic checkpoint serine/threonine kinase (bub1b), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031891202.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCGCAAG CTGGCGATGA ATGGGAGCTA AGCAAAGAAA ATGTGCAACC CCTGCGACAG 
    GGCAGAGTTA TGTCCACTCT GCAGGAGGTG CTCTCACAGC AGGAGAGTGC AAGCCACACT
    GCCATTCAGC AGCAGAAGCA AGCATTTGAG CTGGAACTGC GCTTCTATTC AGGTGATGAT
    CCACTAGATG TCTGGGACAG GTACATCAAG TGGGCTGAGC AGGCATTCCC TCAAGGAGGG
    AAAGAGAGCA ACTTGTGCCC ATTGCTGGAA AGAGCTGTTA AGATATTCCA TGAAGAACAG
    CGATATTATG ATGATCTGCG CTATCTTAGC ATTTGTCTCA AGTTTGCTAA TTTCTGCTCA
    GAGCCTCTGG ATTTGTACAG CTATTTGCAC AGCCAAAGAA TTGGGGTCTC TCATGCCCTA
    CTTTATATCA CTTGGGCCGA ACAGTTTGAA GCTCGAGGTA ATTTCAAGAA AGCTGACTCC
    ATATTTCAGC AGGGCATGCA ACACAAGGCT GAACCACTGG AAAAGCTAGA GATCCATCAT
    CGGCAGTTTC AGGCCCGAGT ATCCCGGCAA ATATTACAAG GAATTTCAGA GGGGCCAGAG
    GCAGAAGAAC CTGAAGTTCC AGAGCCACAG CGCAGCTCAC TGGCAGATCT AAAGTCCAGA
    GGGAAGAACA AAGTGAAAGC ACCCATTAAT CGAGTTGGAG ACTCGATCAA ACCACGTCCT
    CATGGCCTCA GCGTTCAAGC TGCTCCAACA CAGCAAATTC CAAGCCGCTC TCGCTTTGCT
    GTGTTTGATG AAAATGCAGC CTCAGCAGCC CCAGAGCTGC CCTCCTTGAC TCCACAGCAG
    TGGACTGCAC CACCCCCTGC ACGGTCTAAG GAAAATGAAC AGAAAGCTGG ACAATGGAAC
    TCTGGCAGGC CTTCTAGAAA CAGCCATCAG GCTGCTGCTG CCTCTGAACC GCCCCAAACT
    TTGCCCAGTT TCATGCCTTA TGTGGATGAA AGTGCTCAGC AGCAAACAGT AACTCCATGT
    AAAATAAACC CAGCAGTCAC TAGTGTCTTA AGCTCCCGCA AGCCAGGAAA AGAAGAGGAT
    CCTCTGCAAA GGGTACAGAA CAATTCCCAA GGAAAAGAGG AGAAGGTTAT GTACTGCAAG
    GATAAAGTTT ACGCTGGCGT GGAAGAGTTC TCCTTGGAAG AGATAAGGGC AGAAATCTAC
    TTGGCAAAAA TACGGAAGAA GAGAGAAGAC GACCTACAGG AGATTGCACT GAGGAGGCAG
    GAAATGGAGC GACAGATACA GGAGATGGAG AAGCAGCTAA AGGGATCATG TATTGAAAGC
    GAAGAGATCG TAAGTGATCA GCCTGTACAG AATGTTGAAC CTATTATTGC CCCATGTAAT
    TCCAATACAA ATGAGAGTCT GTGTGCATCT CAGCAGGCAC CAGTCATGGA ATTTCCACAT
    TGCTTTGAAA TGGCTGATGC TGCTCCAACA CTGCCAAGGA TGGAGACCCT GCCCGTATTT
    AATGAAACCG GGAACAAGCC CAGCTTGCCA GGTTTGTCTC CAGCACTTTC CTGTGACTTG
    CCCTTCACAA TATTTGATGA ATCTGCAGAA GCTGCACCTA GCATGACTGA CCAGAAGAGA
    ATGGCTGCTC CTGTACGTCG CCCACTTGCT GCTGTTCCTA AGTCCATTGT TAAATCACAA
    GAATCCCAAC TAACCGACAC CCTGGATGGA ACTGAACCCT TAAATGAAGA GGCAATTGTG
    TGTGGCTCAG GTAAAAACAA ATCCTTATTC CCTGACCCAG AAGACACTTG TGATTTTGTG
    AGAGCTGCCC ATCTGGCGTC AACACCTTTC CATAGGGCTA GGGACCAATC AGGCGAATGC
    TTACAGAGTG ACTCGCTTGA TCTGGAAAGG CTCCCGCTTC GAGAGAAGAC ACCTGTGTGC
    GAGGAGTCTT ATAGACAAGA ACTCTGCATA AAGAAACTAA GCCCCATTTT AGAAGCAAGC
    CAAGAAGATA CACGCACATC AGTCTCCTCT GTGTCCTCAA TCTCGAGCAC AACCAGCATG
    TTCACTTCCA GGCCAATTCC AGAGAAGCTC GAGCTGGCTA CTCAGATTAC AGGGGTGTAT
    GAGTCTGAGC CAACCACAGA AGAGCTTCCA CAAGCAGAAG AACTTGCAGA ACTGCATAGG
    CAGTTGCTGG AATCTCTGCC AGAGCTGCTG GTCTCTCCAG AGATCCAACA TGAGGTTGGA
    ACGATGCCTG ATATGAAAGA ACAAGGAGAG GTGGTGCTGG GTTGTGAAAC TTACGGTCTA
    AAAAATGAAG TCATCCTTCA TCCTAACAGC AAGTTGTTTA TGGGTGCCCC TGTGGATTGG
    GATATGGGAG ACATGAAGGC ATTTGCTCTA AAGGTGGATT ATCAGCCACT TCCATGGGAT
    TTCTATGTTA CAATGCAGCT GAAGGAGCGC CTTGGAGATC TATTTGAAAC ATTTTTCATT
    GAACAAACTA ACTGCTTCCT CTACCAGAAT GGGTGCATCA GTCTCTACAA GGATATCAAC
    CGTTTCAGTA TTCAAGAGAT CCTTTTGGAT TCTGAAGAAC ATATCAAAGA AGTAATTGTG
    TTGGTAACAT ACAACTTGTT GAGCCTGGTG GAAAAGCTTC ACTCAGTGGA AATTGTTCAT
    GGAGATCTGA GGCCAGAGAC TCTACTTCTG GATGACAAGA TTTTTGATTT GTCGTCATCT
    CTGGAACTGA AGGGTTTATT TAAGCTGGTA GATTTTTCCC ATAGCGTGGA TCTGAAATTA
    TACCCCATAA TGAGCTCATT ACGTGGCTTC CCCATTGCTC AAACTGAAAG TGGTCAACAA
    TTTCTCAGTC CTCAGTCCTC CCCTTATCAG GTGGACCTTC TTGGCATTGC AGACCTGGTT
    CACCTAATGA TTTTCAGGAA ACCATTGCAG CTGCACCAGG AAAATTCTGT TTGGATGATT
    TGTAAAGAGG TTCCTAGACT GCGTGGTGGA AATCTCTGGA ACAAATTCTT TACAAAGATC
    CTAAATGCAG AAGGCCCAAG CACACGTGTT CTGGGGGAGC TAAAGGGAGA AATGATGGGA
    CAATTTGACA GCTGCTTTCA AGACAAACTT TGCAACTATT TCATCCAGCT GGAGATGAGA
    CTGAACCCGT TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30692
    Clone ID Related Accession (Same CDS sequence) XM_012968790.2
    Accession Version XM_012968790.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3069bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mitotic checkpoint serine/threonine-protein kinase BUB1 beta
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968790.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 2% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCGCAAG CTGGCGATGA ATGGGAGCTA AGCAAAGAAA ATGTGCAACC CCTGCGACAG 
    GGCAGAGTTA TGTCCACTCT GCAGGAGGTG CTCTCACAGC AGGAGAGTGC AAGCCACACT
    GCCATTCAGC AGCAGAAGCA AGCATTTGAG CTGGAACTGC GCTTCTATTC AGGTGATGAT
    CCACTAGATG TCTGGGACAG GTACATCAAG TGGGCTGAGC AGGCATTCCC TCAAGGAGGG
    AAAGAGAGCA ACTTGTGCCC ATTGCTGGAA AGAGCTGTTA AGATATTCCA TGAAGAACAG
    AGATATTATG ATGATCTGCG CTATCTTAGC ATTTGTCTCA AGTTTGCTAA TTTCTGCTCA
    GAGCCTCTGG ATTTGTACAG CTATTTGCAC AGCCAAAGAA TTGGGGTCTC TCATGCCCTA
    CTTTATATCA CTTGGGCCGA ACAGTTTGAA GCTCGAGGTA ATTTCAAGAA AGCTGACTCC
    ATATTTCAGC AGGGCATGCA ACACAAGGCT GAACCACTGG AAAAGCTAGA GATCCATCAT
    CGGCAGTTTC AGGCCCGAGT ATCCCGGCAA ATATTACAAG GAATTTCAGA GGGGCCAGAG
    GCAGAAGAAC CTGAAGTTCC AGAGCCACAG CGCAGCTCAC TGGCAGATCT AAAGTCCAGA
    GGGAAGAACA AAGTGAAAGC ACCCATTAAT CGAGTTGGAG ACTCGATCAA ACCACGTCCT
    CATGGCCTCA GCGTTCAAGC TGCTCCAACA CAGCAAATTC CAAGCCGCTC TCGCTTTGCT
    GTGTTTGATG AAAATGCAGC CTCAGCAGCC CCAGAGCTGC CCTCCTTGAC TCCACAGCAG
    TGGACTGCAC CACCCCCTGC ACGGTCTAAG GAAAATGAAC AGAAAGCTGG ACAATGGAAC
    TCTGGCAGGC CTTCTAGAAA CAGCCATCAG GCTGCTGCTG CCTCTGAACC GCCCCAAACT
    TTGCCCAGTT TCATGCCTTA TGTGGATGAA AGTGCTCAGC AGCAAACAGT AACTCCATGT
    AAAATAAACC CAGCAGTCAC TAGTGTCTTA AGCTCCCGCA AGCCAGGAAA AGAAGAGGAT
    CCTCTGCAAA GGGTACAGAA CAATTCCCAA GGAAAAGAGG AGAAGGTTAT GTACTGCAAG
    GATAAAGTTT ACGCTGGCGT GGAAGAGTTC TCCTTGGAAG AGATAAGGGC AGAAATCTAC
    TTGGCAAAAA TACGGAAGAA GAGAGAAGAC GACCTACAGG AGATTGCACT GAGGAGGCAG
    GAAATGGAGC GACAGATACA GGAGATGGAG AAGCAGCTAA AGGGATCATG TATTGAAAGC
    GAAGAGATCG TAAGTGATCA GGCCAAAACC AGTGGTGGCA CAAATGCCTC TATCTGCCTG
    TCACTCATTC ATAAAGTAAA GTCATACTGG AGGATGGAGA CCCTGCCCGT ATTTAATGAA
    ACAGGGAACA AGCCCAGCTT GCCAGGTTTG TCTCCAGCAC TTTCCTGTGA CTTGCCCTTC
    ACAATATTTG ATGAATCTGC AGAAGCTGCA CCTAGCATGA CTGACCAGAA GAGAATGGCT
    GCTCCTGTAC GTCGCCCACT TGCTGCTGTT CCTAAGTCCA TTGTTAAATC ACAAGAATCC
    CAACTAACCG ACACCCTGGA TGGAATTGAA CCCTTAAATG AAGAGGCAAT TGTGTGTGGC
    TCAGGTAAAA ACAAATCCTT ATTCCCTGAC CCAGAAGACA CTTGTGATTT TGTGAGAGCT
    GCCCATCTGG CGTCAACACC TTTCCATAGG GCTAGGGACC AATCAGGCGA ATGCTTACAG
    AGTGACTCGC TTGATCTGGA AAGGCTCCCG CTTCGAGAGA AGACACCTGT GTGCGAGGAG
    TCTTATAGAC AAGAACTCTG CATAAAGAAA CTAAGCCCCA TTTTAGAAGC AAGCCAAGAA
    GATACACGCA CATCAGTCTC CTCTGTGTCC TCAATCTCGA GCACAACCAG CATGTTCACT
    TCCAGGCCAA TTCCAGAGAA GCTCGAGCTG GCTACTCAGA TTACAGGGGT GTATGAGTCT
    GAGCCAACCA CAGAAGAGCT TCCACAAGCA GAAGAACTTG CAGAACTGCA TAGGCAGTTG
    CTGGAATCTC TGCCAGAGCT GCTGGTCTCT CCAGAGATCC AACATGAGGT TGGAACGATG
    CCTGATATGA AAGAACAAGG AGAGGTGGTG CTGGGTTGTG AAACTTACGG TCTAAAAAAT
    GAAGTCATCC TTCATCCTAA CAGCAAGTTG TTTATGGGTG CCCCTGTGGA TTGGGATATG
    GGAGACATGA AGGCATTTGC TCTAAAGGTG GATTATCAGC CACTTCCATG GGATTTCTAT
    GTTACAATGC AGCTGAAGGA GCGCCTTGGA GATCTATTTG AAACATTTTT CATTGAACAA
    ACTAACTGCT TCCTCTACCA GAATGGGTGC ATCAGTCTCT ACAAGGATAT CAACCGTTTC
    AGTATTCAAG AGATCCTTTT GGATTCTGAA GAACATATCA AAGAAGTAAT TGTGTTGGTA
    ACATACAACT TGTTGAGCCT GGTGGAAAAG CTTCACTCAG TGGAAATTGT TCATGGAGAT
    CTGAGGCCAG AGACTCTACT TCTGGATGAC AAGATTTTTG ATTTGTCGTC ATCTCTGGAA
    CTGGAGGGTT TATTTAAGCT GGTAGATTTT TCCCATAGCG TGGATCTGAA ATTATACCCC
    ATAATGAGCT CATTACGTGG CTTCCCCATT GCTCAAACTG AAAGTGGTCA ACAATTTCTC
    AGTCCTCAGT CCTCCCCTTA TCAGGTGGAC CTTCTTGGCA TTGCAGACCT GGTTCACCTA
    ATGATTTTCA GGAAACCATT GCAGCTGCAC CAGGAAAATT CTGTTTGGAT GATTTGTAAA
    GAGGTTCCTA GACTGCGTGG TGGAAATCTC TGGAACAAAT TCTTTACAAA GATCCTAAAT
    GCAGAAGGCC CAAGCACACG TGTTCTGGGG GAGCTAAAGG GAGAAATGAT GGGACAATTT
    GACAGCTGCT TTCAAGACAA ACTTTGCAAC TATTTCATCC AGCTGGAGAT GAGACTGAAC
    CCGTTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012824244.1
    CDS152..3220
    Translation

    Target ORF information:

    RefSeq Version XM_012968790.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis BUB1B, mitotic checkpoint serine/threonine kinase (bub1b), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012968790.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCGCAAG CTGGCGATGA ATGGGAGCTA AGCAAAGAAA ATGTGCAACC CCTGCGACAG 
    GGCAGAGTTA TGTCCACTCT GCAGGAGGTG CTCTCACAGC AGGAGAGTGC AAGCCACACT
    GCCATTCAGC AGCAGAAGCA AGCATTTGAG CTGGAACTGC GCTTCTATTC AGGTGATGAT
    CCACTAGATG TCTGGGACAG GTACATCAAG TGGGCTGAGC AGGCATTCCC TCAAGGAGGG
    AAAGAGAGCA ACTTGTGCCC ATTGCTGGAA AGAGCTGTTA AGATATTCCA TGAAGAACAG
    AGATATTATG ATGATCTGCG CTATCTTAGC ATTTGTCTCA AGTTTGCTAA TTTCTGCTCA
    GAGCCTCTGG ATTTGTACAG CTATTTGCAC AGCCAAAGAA TTGGGGTCTC TCATGCCCTA
    CTTTATATCA CTTGGGCCGA ACAGTTTGAA GCTCGAGGTA ATTTCAAGAA AGCTGACTCC
    ATATTTCAGC AGGGCATGCA ACACAAGGCT GAACCACTGG AAAAGCTAGA GATCCATCAT
    CGGCAGTTTC AGGCCCGAGT ATCCCGGCAA ATATTACAAG GAATTTCAGA GGGGCCAGAG
    GCAGAAGAAC CTGAAGTTCC AGAGCCACAG CGCAGCTCAC TGGCAGATCT AAAGTCCAGA
    GGGAAGAACA AAGTGAAAGC ACCCATTAAT CGAGTTGGAG ACTCGATCAA ACCACGTCCT
    CATGGCCTCA GCGTTCAAGC TGCTCCAACA CAGCAAATTC CAAGCCGCTC TCGCTTTGCT
    GTGTTTGATG AAAATGCAGC CTCAGCAGCC CCAGAGCTGC CCTCCTTGAC TCCACAGCAG
    TGGACTGCAC CACCCCCTGC ACGGTCTAAG GAAAATGAAC AGAAAGCTGG ACAATGGAAC
    TCTGGCAGGC CTTCTAGAAA CAGCCATCAG GCTGCTGCTG CCTCTGAACC GCCCCAAACT
    TTGCCCAGTT TCATGCCTTA TGTGGATGAA AGTGCTCAGC AGCAAACAGT AACTCCATGT
    AAAATAAACC CAGCAGTCAC TAGTGTCTTA AGCTCCCGCA AGCCAGGAAA AGAAGAGGAT
    CCTCTGCAAA GGGTACAGAA CAATTCCCAA GGAAAAGAGG AGAAGGTTAT GTACTGCAAG
    GATAAAGTTT ACGCTGGCGT GGAAGAGTTC TCCTTGGAAG AGATAAGGGC AGAAATCTAC
    TTGGCAAAAA TACGGAAGAA GAGAGAAGAC GACCTACAGG AGATTGCACT GAGGAGGCAG
    GAAATGGAGC GACAGATACA GGAGATGGAG AAGCAGCTAA AGGGATCATG TATTGAAAGC
    GAAGAGATCG TAAGTGATCA GGCCAAAACC AGTGGTGGCA CAAATGCCTC TATCTGCCTG
    TCACTCATTC ATAAAGTAAA GTCATACTGG AGGATGGAGA CCCTGCCCGT ATTTAATGAA
    ACAGGGAACA AGCCCAGCTT GCCAGGTTTG TCTCCAGCAC TTTCCTGTGA CTTGCCCTTC
    ACAATATTTG ATGAATCTGC AGAAGCTGCA CCTAGCATGA CTGACCAGAA GAGAATGGCT
    GCTCCTGTAC GTCGCCCACT TGCTGCTGTT CCTAAGTCCA TTGTTAAATC ACAAGAATCC
    CAACTAACCG ACACCCTGGA TGGAATTGAA CCCTTAAATG AAGAGGCAAT TGTGTGTGGC
    TCAGGTAAAA ACAAATCCTT ATTCCCTGAC CCAGAAGACA CTTGTGATTT TGTGAGAGCT
    GCCCATCTGG CGTCAACACC TTTCCATAGG GCTAGGGACC AATCAGGCGA ATGCTTACAG
    AGTGACTCGC TTGATCTGGA AAGGCTCCCG CTTCGAGAGA AGACACCTGT GTGCGAGGAG
    TCTTATAGAC AAGAACTCTG CATAAAGAAA CTAAGCCCCA TTTTAGAAGC AAGCCAAGAA
    GATACACGCA CATCAGTCTC CTCTGTGTCC TCAATCTCGA GCACAACCAG CATGTTCACT
    TCCAGGCCAA TTCCAGAGAA GCTCGAGCTG GCTACTCAGA TTACAGGGGT GTATGAGTCT
    GAGCCAACCA CAGAAGAGCT TCCACAAGCA GAAGAACTTG CAGAACTGCA TAGGCAGTTG
    CTGGAATCTC TGCCAGAGCT GCTGGTCTCT CCAGAGATCC AACATGAGGT TGGAACGATG
    CCTGATATGA AAGAACAAGG AGAGGTGGTG CTGGGTTGTG AAACTTACGG TCTAAAAAAT
    GAAGTCATCC TTCATCCTAA CAGCAAGTTG TTTATGGGTG CCCCTGTGGA TTGGGATATG
    GGAGACATGA AGGCATTTGC TCTAAAGGTG GATTATCAGC CACTTCCATG GGATTTCTAT
    GTTACAATGC AGCTGAAGGA GCGCCTTGGA GATCTATTTG AAACATTTTT CATTGAACAA
    ACTAACTGCT TCCTCTACCA GAATGGGTGC ATCAGTCTCT ACAAGGATAT CAACCGTTTC
    AGTATTCAAG AGATCCTTTT GGATTCTGAA GAACATATCA AAGAAGTAAT TGTGTTGGTA
    ACATACAACT TGTTGAGCCT GGTGGAAAAG CTTCACTCAG TGGAAATTGT TCATGGAGAT
    CTGAGGCCAG AGACTCTACT TCTGGATGAC AAGATTTTTG ATTTGTCGTC ATCTCTGGAA
    CTGGAGGGTT TATTTAAGCT GGTAGATTTT TCCCATAGCG TGGATCTGAA ATTATACCCC
    ATAATGAGCT CATTACGTGG CTTCCCCATT GCTCAAACTG AAAGTGGTCA ACAATTTCTC
    AGTCCTCAGT CCTCCCCTTA TCAGGTGGAC CTTCTTGGCA TTGCAGACCT GGTTCACCTA
    ATGATTTTCA GGAAACCATT GCAGCTGCAC CAGGAAAATT CTGTTTGGAT GATTTGTAAA
    GAGGTTCCTA GACTGCGTGG TGGAAATCTC TGGAACAAAT TCTTTACAAA GATCCTAAAT
    GCAGAAGGCC CAAGCACACG TGTTCTGGGG GAGCTAAAGG GAGAAATGAT GGGACAATTT
    GACAGCTGCT TTCAAGACAA ACTTTGCAAC TATTTCATCC AGCTGGAGAT GAGACTGAAC
    CCGTTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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