ORF » Species Summary » Xenopus tropicalis » mib1 cDNA ORF clone

mib1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following mib1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mib1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa24081 NM_001129935.1
Latest version!		 Xenopus tropicalis mindbomb E3 ubiquitin protein ligase 1 (mib1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa32282 XM_012964358.2
Latest version!		 Xenopus tropicalis mindbomb E3 ubiquitin protein ligase 1 (mib1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    mib1 ( NM_001129935.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001123407.1 Xenopus tropicalis mindbomb E3 ubiquitin protein ligase 1 (mib1), mRNA.
    Price & Availability↑
    CloneID OXa24081
    Clone ID Related Accession (Same CDS sequence) NM_001129935.1
    Accession Version NM_001129935.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3033bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase MIB1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC167461.1. ##Evidence-Data-START## Transcript exon combination :: BC167461.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGCGGCT CCCGTAATAA CCGGGTGATG GTGGAGGGAG TCGGGGCTCG GGTTGCGCGG 
    GGCCCGGATT GGAAATGGGG GAAACAGGAC GGAGGAGAGG GTCATGTGGG CACCGTACGG 
    AGCTTCGAAA GCCCAGAAGA GGTGGTGGTG GTCTGGGACA ATGGCACCGC GGCCAATTAC 
    CGCTGCTCCG GGGCCTACGA CCTGAGGATA CTGGACAGTG CGCCTACTGG CATTAAACAT 
    GATGGGACCA TGTGTGATAC ATGCCGCCAG CAACCTATTA TTGGAATCCG TTGGAAATGT 
    GCTGAATGTA CTAATTATGA TCTATGCACT GTGTGCTACC ATGGGGATAA GCATCACCTT 
    AGACATCGCT TTTACAGGAT CACAACACCG GGAAGCGAGA GGGTGCTCCT GGAGTCTCGG 
    CGCAAATCAA AGAAAATTAC GGCAAGAGGT ATCTTTGCAG GCGCTAGAGT AGTGCGTGGA 
    GTAGATTGGC AGTGGGAAGA CCAAGATGGT GGTAATGGAA GGCGAGGCAA AGTAACAGAA 
    ATCCAAGATT GGAGCGCATC AAGCCCACAC AGTGCAGCAT ATGTCCTGTG GGACAATGGA 
    GCAAAAAATC TGTATAGGGT TGGATTTGAA GGCATGTCTG ACCTGAAATG TGTCCAGGAT 
    GCTAAAGGTG GTTCTTTTTA CAGAGACCAT TGTCCTGTGT TGGGTGAACA GAATGGGAAT 
    CGGAATCCTG GTGGCCTGCT CATTGGTGAT CTAGTTAATA TTGATTTAGA ACTGGAAATT 
    GTTCAGTCAT TGCAACATGG TCATGGAGGC TGGACCGATG GCATGTTCGA AACTCTAACC 
    ACTACTGGTA CTGTGTGTGG CATTGATGAA GACCATGATA TTGTAGTGCA ATACCCCAGT 
    GGAAACAGGT GGACATTTAA TCCAGCTGTT CTCACCAAGG CAAATGTTGT TCGCAGTGGC 
    GATGCTGCTC AAGGTGCAGA AGGTGGGACA TCACCTTTTC AAGTGGGTGA TCTTGTGCAA 
    ATATGTTATG ACTTAGAGAG AATAAAACTT CTTCAAAGAG GCCATGGTGA ATGGGCTGAA 
    GCTATGCTAC CAACTCTAGG TAAAGTTGGC AGAGTACAAC AGATTTATTC AGACAACGAT 
    TTGAAAGTTG AAGTTTGTGG GACATCTTGG ACATATAATC CAGCTGCTGT TTCAAGAGTT 
    GCTTCTGTGG GATCAGCCAT TAGTAATGCC ACTGGTGAAA GACTGTCACA GCTATTGAAG 
    AAATTGTTTG AGACTCAGGA ATCAGGCGAC CTTAATGAGG AGCTGGTGAA AGCTGCTGCA 
    AATGGAGATG TGGCTAAAGT GGAGGACTTG CTGAAAAGAC AGGATGTAGA TGTAAATGGA 
    CAATGCGCTG GACACACAGC AATGCAAGCT GCCAGCCAGA ATGGACATGT AGATATCTTG 
    AAATTGCTTC TAAAGCACAG TGTAGATGTA GAAGCTGAGG ATAAAGATGG AGATCGGGCA 
    GTGCACCATG CAGCTTTTGG AGATGAAGGC ACTGTTGTTG AAGTGCTGCA TAGAGGGGGA 
    GCGGATTTAA ATGCACGGAA CAAGCGCAGA CAGACACCTC TTCATATTGC AGTTAATAAA 
    GGCCACTTGC AAGTTGTAAA AAAATTATTG GATTTTGGCT GCCACCCAAG CCTACAGGAT 
    TCAGAAGGTG ATACTCCGCT CCATGATGCT ATCAGCAAAA AACGTGATGA TATATTGGCT 
    GTACTTTTAG AGGCTGGAGC AGATGTTACC ATCACAAACA ACAATGGGTT CAATGCTCTT 
    CATCATGCTG CATTGCGGGG AAATCCCAGT GCTATGCGGG TCCTGCTCTC CAAGTTGCCC 
    CGTCCGTGGA TTGTAGATGA AAAGAAAGAT GATGGATACA CTGCTTTGCA CCTTGCTGCA 
    CTTAATAATC ATGTTGAAGT AGCGGAGTTA TTGGTTCATC AGGGAAACGC AAACCTGGAC 
    ATTCAGAATG TAAACCAGCA GACTGCTCTT CACTTGGCTG TGGAACGTCA GCATACACAA 
    ATTGTCAGAC TGTTGGTTCG TGCAGAAGCT AAACTGGACA TACAAGATAA AGATGGGGAT 
    ACACCATTAC ATGAAGCCCT TCGGCACCAT ACTTTGTCAC AGTTGCGGCA GCTTCAAGAC 
    ATGCAGGATG TTGGCAAGGT TGATACTACC TGGGAGCCAT CAAAGAACAC ATTAATAATG 
    GGACTTGGTA CTCAAGGAGC TGAGAAGAAA AGTGCAGCCT CCATTGCCTG CTTTTTGGCT 
    GCCAATGGAG CTGACCTTAC AATCCGTAAC AAAAAAGGAC AGTCCCCTCT TGACCTCTGT 
    CCAGACCCCA ACCTTTGCAA AGCTTTGGCT AAATGTCACA AGGAGAAATC CAGTGGTCAA 
    GTAGGATCCC ATAGTCCCTC TATGATAAGC AATGATTCCG AGACATTAGA AGAATGTATG 
    GTGTGTTCAG ATTTAAAGAG AGATACTCTT TTTGGGCCAT GTGGACACAT TGCTACATGT 
    TCTTTATGTT CTCCACGTGT TAAGAAATGC CTCATATGCA AAGAACAAGT CCAGTCCAGG 
    ACAAAGATAG AAGAATGTGT GGTGTGTTCA GATAAGAAGG CCGCGGTGCT CTTCCAGCCT 
    TGTGGCCATA TGTGTGCTTG TGAAAACTGT GCAAGTCTAA TGAAGAAATG TGTTCAGTGC 
    CGTGCTGTGG TTGAGCGAAG AGTGCCTTTT GTTATGTGTT GCAGTGGCAA AGGTACAGAG 
    GATGTCACAG ATGATATAGC CGGCGGAAAC ATACAAGCTT TACAGAAGGA CAAGGACAAT 
    ACCAATGTCA ATGCAGATGT GCAGAAGCTG CAGCAGCAGT TACAGGACAT AAAGGAACAG 
    ACAATGTGCC CTGTTTGTTT GGATCGGTTG AAGAATATGA TCTTCATGTG TGGACATGGA 
    ACTTGCCAGC TGTGTGGAGA TCGAATGAGT GAATGCCCCA TTTGTCGGAA GGCCATAGAG 
    AGAAGAATAT TATTATACAG TATGTCTTGT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001123407.1
    CDS184..3216
    Translation

    Target ORF information:

    RefSeq Version NM_001129935.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mindbomb E3 ubiquitin protein ligase 1 (mib1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001129935.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGCGGCT CCCGTAATAA CCGGGTGATG GTGGAGGGAG TCGGGGCTCG GGTTGCGCGG 
    GGCCCGGATT GGAAATGGGG GAAACAGGAC GGAGGAGAGG GTCATGTGGG CACCGTACGG 
    AGCTTCGAAA GCCCAGAAGA GGTGGTGGTG GTCTGGGACA ATGGCACCGC GGCCAATTAC 
    CGCTGCTCCG GGGCCTACGA CCTGAGGATA CTGGACAGTG CGCCTACTGG CATTAAACAT 
    GATGGGACCA TGTGTGATAC ATGCCGCCAG CAACCTATTA TTGGAATCCG TTGGAAATGT 
    GCTGAATGTA CTAATTATGA TCTATGCACT GTGTGCTACC ATGGGGATAA GCATCACCTT 
    AGACATCGCT TTTACAGGAT CACAACACCG GGAAGCGAGA GGGTGCTCCT GGAGTCTCGG 
    CGCAAATCAA AGAAAATTAC GGCAAGAGGT ATCTTTGCAG GCGCTAGAGT AGTGCGTGGA 
    GTAGATTGGC AGTGGGAAGA CCAAGATGGT GGTAATGGAA GGCGAGGCAA AGTAACAGAA 
    ATCCAAGATT GGAGCGCATC AAGCCCACAC AGTGCAGCAT ATGTCCTGTG GGACAATGGA 
    GCAAAAAATC TGTATAGGGT TGGATTTGAA GGCATGTCTG ACCTGAAATG TGTCCAGGAT 
    GCTAAAGGTG GTTCTTTTTA CAGAGACCAT TGTCCTGTGT TGGGTGAACA GAATGGGAAT 
    CGGAATCCTG GTGGCCTGCT CATTGGTGAT CTAGTTAATA TTGATTTAGA ACTGGAAATT 
    GTTCAGTCAT TGCAACATGG TCATGGAGGC TGGACCGATG GCATGTTCGA AACTCTAACC 
    ACTACTGGTA CTGTGTGTGG CATTGATGAA GACCATGATA TTGTAGTGCA ATACCCCAGT 
    GGAAACAGGT GGACATTTAA TCCAGCTGTT CTCACCAAGG CAAATGTTGT TCGCAGTGGC 
    GATGCTGCTC AAGGTGCAGA AGGTGGGACA TCACCTTTTC AAGTGGGTGA TCTTGTGCAA 
    ATATGTTATG ACTTAGAGAG AATAAAACTT CTTCAAAGAG GCCATGGTGA ATGGGCTGAA 
    GCTATGCTAC CAACTCTAGG TAAAGTTGGC AGAGTACAAC AGATTTATTC AGACAACGAT 
    TTGAAAGTTG AAGTTTGTGG GACATCTTGG ACATATAATC CAGCTGCTGT TTCAAGAGTT 
    GCTTCTGTGG GATCAGCCAT TAGTAATGCC ACTGGTGAAA GACTGTCACA GCTATTGAAG 
    AAATTGTTTG AGACTCAGGA ATCAGGCGAC CTTAATGAGG AGCTGGTGAA AGCTGCTGCA 
    AATGGAGATG TGGCTAAAGT GGAGGACTTG CTGAAAAGAC AGGATGTAGA TGTAAATGGA 
    CAATGCGCTG GACACACAGC AATGCAAGCT GCCAGCCAGA ATGGACATGT AGATATCTTG 
    AAATTGCTTC TAAAGCACAG TGTAGATGTA GAAGCTGAGG ATAAAGATGG AGATCGGGCA 
    GTGCACCATG CAGCTTTTGG AGATGAAGGC ACTGTTGTTG AAGTGCTGCA TAGAGGGGGA 
    GCGGATTTAA ATGCACGGAA CAAGCGCAGA CAGACACCTC TTCATATTGC AGTTAATAAA 
    GGCCACTTGC AAGTTGTAAA AAAATTATTG GATTTTGGCT GCCACCCAAG CCTACAGGAT 
    TCAGAAGGTG ATACTCCGCT CCATGATGCT ATCAGCAAAA AACGTGATGA TATATTGGCT 
    GTACTTTTAG AGGCTGGAGC AGATGTTACC ATCACAAACA ACAATGGGTT CAATGCTCTT 
    CATCATGCTG CATTGCGGGG AAATCCCAGT GCTATGCGGG TCCTGCTCTC CAAGTTGCCC 
    CGTCCGTGGA TTGTAGATGA AAAGAAAGAT GATGGATACA CTGCTTTGCA CCTTGCTGCA 
    CTTAATAATC ATGTTGAAGT AGCGGAGTTA TTGGTTCATC AGGGAAACGC AAACCTGGAC 
    ATTCAGAATG TAAACCAGCA GACTGCTCTT CACTTGGCTG TGGAACGTCA GCATACACAA 
    ATTGTCAGAC TGTTGGTTCG TGCAGAAGCT AAACTGGACA TACAAGATAA AGATGGGGAT 
    ACACCATTAC ATGAAGCCCT TCGGCACCAT ACTTTGTCAC AGTTGCGGCA GCTTCAAGAC 
    ATGCAGGATG TTGGCAAGGT TGATACTACC TGGGAGCCAT CAAAGAACAC ATTAATAATG 
    GGACTTGGTA CTCAAGGAGC TGAGAAGAAA AGTGCAGCCT CCATTGCCTG CTTTTTGGCT 
    GCCAATGGAG CTGACCTTAC AATCCGTAAC AAAAAAGGAC AGTCCCCTCT TGACCTCTGT 
    CCAGACCCCA ACCTTTGCAA AGCTTTGGCT AAATGTCACA AGGAGAAATC CAGTGGTCAA 
    GTAGGATCCC ATAGTCCCTC TATGATAAGC AATGATTCCG AGACATTAGA AGAATGTATG 
    GTGTGTTCAG ATTTAAAGAG AGATACTCTT TTTGGGCCAT GTGGACACAT TGCTACATGT 
    TCTTTATGTT CTCCACGTGT TAAGAAATGC CTCATATGCA AAGAACAAGT CCAGTCCAGG 
    ACAAAGATAG AAGAATGTGT GGTGTGTTCA GATAAGAAGG CCGCGGTGCT CTTCCAGCCT 
    TGTGGCCATA TGTGTGCTTG TGAAAACTGT GCAAGTCTAA TGAAGAAATG TGTTCAGTGC 
    CGTGCTGTGG TTGAGCGAAG AGTGCCTTTT GTTATGTGTT GCAGTGGCAA AGGTACAGAG 
    GATGTCACAG ATGATATAGC CGGCGGAAAC ATACAAGCTT TACAGAAGGA CAAGGACAAT 
    ACCAATGTCA ATGCAGATGT GCAGAAGCTG CAGCAGCAGT TACAGGACAT AAAGGAACAG 
    ACAATGTGCC CTGTTTGTTT GGATCGGTTG AAGAATATGA TCTTCATGTG TGGACATGGA 
    ACTTGCCAGC TGTGTGGAGA TCGAATGAGT GAATGCCCCA TTTGTCGGAA GGCCATAGAG 
    AGAAGAATAT TATTATACAG TATGTCTTGT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    mib1 ( XM_012964358.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819812.1 Xenopus tropicalis mindbomb E3 ubiquitin protein ligase 1 (mib1), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OXa32282
    Clone ID Related Accession (Same CDS sequence) XM_012964358.2
    Accession Version XM_012964358.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3021bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase MIB1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964358.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGCGGCT CCCGTAATAA CCGGGTGATG GTGGAGGGGG TCGGGGCTCG GGTTGCGCGG 
    GGCCCGGATT GGAAATGGGG GAAACAGGAC GGAGGAGAGG GTCATGTGGG CACCGTACGG 
    AGCTTCGAAA GCCCAGAAGA GGTGGTGGTG GTCTGGGACA ATGGCACCGC GGCCAATTAC 
    CGCTGCTCGG GGGCCTACGA CCTGAGGATA CTGGACAGTG CGCCTACTGG CATTAAACAT 
    GATGGGACCA TGTGTGATAC ATGCCGCCAG CAACCTATTA TTGGAATCCG TTGGAAATGT 
    GCTGAATGTA CTAATTATGA TCTATGCACT GTGTGCTACC ATGGGGATAA GCATCACCTT 
    AGACATCGCT TTTACAGGAT CACAACACCG GGAAGCGAGA GGGTGCTCCT GGAGTCTCGG 
    CGCAAATCAA AGAAAATTAC GGCAAGAGGT ATCTTTGCAG GCGCTAGAGT AGTGCGTGGA 
    GTAGATTGGC AGTGGGAAGA CCAAGATGGT GGTAATGGAA GGCGAGGCAA AGTAACAGAA 
    ATCCAAGATT GGAGCGCATC AAGCCCACAC AGTGCAGCAT ATGTCCTGTG GGACAATGGA 
    GCAAAAAATC TGTATAGGGT TGGATTTGAA GGCATGTCTG ACCTGAAATG TGTCCAGGAT 
    GCTAAAGGTG GTTCTTTTTA CAGAGACCAT TGTCCTGTGT TGGGTGAACA GAATGGGAAT 
    CGGAATCCTG GTGGCCTGCT CATTGGTGAT CTAGTTAATA TTGATTTAGA ACTGGAAATT 
    GTTCAGTCAT TGCAACATGG TCATGGAGGC TGGACCGATG GCATGTTCGA AACTCTAACC 
    ACTACTGGTA CTGTGTGTGG CATTGATGAA GACCATGATA TTGTAGTGCA ATACCCCAGT 
    GGAAACAGGT GGACATTTAA TCCAGCTGTT CTCACCAAGG CAAATGTTGT TCGCAGTGGC 
    GATGCTGCTC AAGGTGCAGA AGGTGGGACA TCACCTTTTC AAGTGGGTGA TCTTGTGCAA 
    ATATGTTATG ACTTAGAGAG AATAAAACTT CTTCAAAGAG GCCATGGTGA ATGGGCTGAA 
    GCTATGCTAC CAACTCTAGG TAAAGTTGGC AGAGTACAAC AGATTTATTC AGACAACGAT 
    TTGAAAGTTG AAGTTTGTGG GACATCTTGG ACATATAATC CAGCTGCTGT TTCAAGAGTT 
    GCTTCTGTGG GATCAGCCAT TAGTAATGCC ACTGGTGAAA GACTGTCACA GCTATTGAAG 
    AAATTGTTTG AGACTCAGGA ATCAGGCGAC CTTAATGAGG AGCTGGTGAA AGCTGCTGCA 
    AATGGAGATG TGGCTAAAGT GGAGGACTTG CTGAAAAGAC AGGATGTAGA TGTAAATGGA 
    CAATGCGCTG GACACACAGC AATGCAAGCT GCCAGCCAGA ATGGACATGT AGATATCTTG 
    AAATTGCTTC TAAAGCACAG TGTAGATGTA GAAGCTGAGG ATAAAGATGG AGATCGGGCA 
    GTGCACCATG CAGCTTTTGG AGATGAAGGC ACTGTTGTTG AAGTGCTGCA TAGAGGGGGA 
    GCGGATTTAA ATGCACGGAA CAAGCGCAGA CAGACACCTC TTCATATTGC AGTTAATAAA 
    GGCCACTTGC AAGTTGTAAA AAAATTATTG GATTTTGGCT GCCACCCAAG CCTACAGGAT 
    TCAGAAGGTG ATACTCCGCT CCATGATGCT ATCAGCAAAA AACGTGATGA TATATTGGCT 
    GTACTTTTAG AGGCTGGAGC AGATGTTACC ATCACAAACA ACAATGGGTT CAATGCTCTT 
    CATCATGCTG CATTGCGGGG AAATCCCAGT GCTATGCGGG TCCTGCTCTC CAAGTTGCCC 
    CGTCCGTGGA TTGTAGATGA AAAGAAAGAT GATGGATACA CTGCTTTGCA CCTTGCTGCA 
    CTTAATAATC ATGTTGAAGT AGCGGAGTTA TTGGTTCATC AGGGAAACGC AAACCTGGAC 
    ATTCAGAATG TAAACCAGCA GACTGCTCTT CACTTGGCTG TGGAACGTCA GCATACACAA 
    ATTGTCAGAC TGTTGGTTCG TGCAGAAGCT AAACTGGACA TACAAGATAA AGATGGGGAT 
    ACACCATTAC ATGAAGCCCT TCGGCACCAT ACTTTGTCAC AGTTGCGGCA GCTTCAAGAC 
    ATGCAGGATG TTGGCAAGGT TGATACTACC TGGGAGCCAT CAAAGAACAC ATTAATAATG 
    GGACTTGGTA CTCAAGGAGC TGAGAAGAAA AGTGCAGCCT CCATTGCCTG CTTTTTGGCT 
    GCCAATGGAG CTGACCTTAC AATCCGTAAC AAAAAAGGAC AGTCCCCTCT TGACCTCTGT 
    CCAGACCCCA ACCTTTGCAA AGCTTTGGCT AAATGTCACA AGGAGAAATC CAGTGGTCAA 
    GTAGGATCCC ATAGTCCCTC TATGATAAGC AATGATTCTG AGACATTAGA AGAATGTATG 
    GTGTGTTCAG ATTTAAAGAG AGATACTCTT TTTGGGCCAT GTGGACACAT TGCTACATGT 
    TCTTTATGTT CTCCACGTGT TAAGAAATGC CTCATATGCA AAGAACAAGT CCAGTCCAGG 
    ACAAAGATAG AAGAATGTGT GGTGTGTTCA GATAAGAAGG CCGCGGTGCT CTTCCAGCCT 
    TGTGGCCATA TGTGTGCTTG TGAAAACTGT GCAAGTCTAA TGAAGAAATG TGTTCAGTGC 
    CGTGCTGTGG TTGAGCGAAG AGTGCCTTTT GTTATGTGTT GCAGTGGCAA AGGTACAGAG 
    GATGTCACAG ATGATATAGC CGGCGGAAAC ATACAAGCTT TACAGAAGGA CAAGGACAAT 
    ACCAATGTCA ATGCAGATGT GCAGAAGCTG CAGCAGCAGT TACAGGACAT AAAGGAACAG 
    ACAATGTGCC CTGTTTGTTT GGATCGGTTG AAGAATATGA TCTTCATGTG TGGACATGGA 
    ACTTGCCAGC TGTGTGGAGA TCGAATGAGT GAATGCCCCA TTTGTCGGAA GGCCATAGAG 
    AGAAGAATAT TATTGTACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012819812.1
    CDS280..3300
    Translation

    Target ORF information:

    RefSeq Version XM_012964358.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mindbomb E3 ubiquitin protein ligase 1 (mib1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012964358.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGCGGCT CCCGTAATAA CCGGGTGATG GTGGAGGGGG TCGGGGCTCG GGTTGCGCGG 
    GGCCCGGATT GGAAATGGGG GAAACAGGAC GGAGGAGAGG GTCATGTGGG CACCGTACGG 
    AGCTTCGAAA GCCCAGAAGA GGTGGTGGTG GTCTGGGACA ATGGCACCGC GGCCAATTAC 
    CGCTGCTCGG GGGCCTACGA CCTGAGGATA CTGGACAGTG CGCCTACTGG CATTAAACAT 
    GATGGGACCA TGTGTGATAC ATGCCGCCAG CAACCTATTA TTGGAATCCG TTGGAAATGT 
    GCTGAATGTA CTAATTATGA TCTATGCACT GTGTGCTACC ATGGGGATAA GCATCACCTT 
    AGACATCGCT TTTACAGGAT CACAACACCG GGAAGCGAGA GGGTGCTCCT GGAGTCTCGG 
    CGCAAATCAA AGAAAATTAC GGCAAGAGGT ATCTTTGCAG GCGCTAGAGT AGTGCGTGGA 
    GTAGATTGGC AGTGGGAAGA CCAAGATGGT GGTAATGGAA GGCGAGGCAA AGTAACAGAA 
    ATCCAAGATT GGAGCGCATC AAGCCCACAC AGTGCAGCAT ATGTCCTGTG GGACAATGGA 
    GCAAAAAATC TGTATAGGGT TGGATTTGAA GGCATGTCTG ACCTGAAATG TGTCCAGGAT 
    GCTAAAGGTG GTTCTTTTTA CAGAGACCAT TGTCCTGTGT TGGGTGAACA GAATGGGAAT 
    CGGAATCCTG GTGGCCTGCT CATTGGTGAT CTAGTTAATA TTGATTTAGA ACTGGAAATT 
    GTTCAGTCAT TGCAACATGG TCATGGAGGC TGGACCGATG GCATGTTCGA AACTCTAACC 
    ACTACTGGTA CTGTGTGTGG CATTGATGAA GACCATGATA TTGTAGTGCA ATACCCCAGT 
    GGAAACAGGT GGACATTTAA TCCAGCTGTT CTCACCAAGG CAAATGTTGT TCGCAGTGGC 
    GATGCTGCTC AAGGTGCAGA AGGTGGGACA TCACCTTTTC AAGTGGGTGA TCTTGTGCAA 
    ATATGTTATG ACTTAGAGAG AATAAAACTT CTTCAAAGAG GCCATGGTGA ATGGGCTGAA 
    GCTATGCTAC CAACTCTAGG TAAAGTTGGC AGAGTACAAC AGATTTATTC AGACAACGAT 
    TTGAAAGTTG AAGTTTGTGG GACATCTTGG ACATATAATC CAGCTGCTGT TTCAAGAGTT 
    GCTTCTGTGG GATCAGCCAT TAGTAATGCC ACTGGTGAAA GACTGTCACA GCTATTGAAG 
    AAATTGTTTG AGACTCAGGA ATCAGGCGAC CTTAATGAGG AGCTGGTGAA AGCTGCTGCA 
    AATGGAGATG TGGCTAAAGT GGAGGACTTG CTGAAAAGAC AGGATGTAGA TGTAAATGGA 
    CAATGCGCTG GACACACAGC AATGCAAGCT GCCAGCCAGA ATGGACATGT AGATATCTTG 
    AAATTGCTTC TAAAGCACAG TGTAGATGTA GAAGCTGAGG ATAAAGATGG AGATCGGGCA 
    GTGCACCATG CAGCTTTTGG AGATGAAGGC ACTGTTGTTG AAGTGCTGCA TAGAGGGGGA 
    GCGGATTTAA ATGCACGGAA CAAGCGCAGA CAGACACCTC TTCATATTGC AGTTAATAAA 
    GGCCACTTGC AAGTTGTAAA AAAATTATTG GATTTTGGCT GCCACCCAAG CCTACAGGAT 
    TCAGAAGGTG ATACTCCGCT CCATGATGCT ATCAGCAAAA AACGTGATGA TATATTGGCT 
    GTACTTTTAG AGGCTGGAGC AGATGTTACC ATCACAAACA ACAATGGGTT CAATGCTCTT 
    CATCATGCTG CATTGCGGGG AAATCCCAGT GCTATGCGGG TCCTGCTCTC CAAGTTGCCC 
    CGTCCGTGGA TTGTAGATGA AAAGAAAGAT GATGGATACA CTGCTTTGCA CCTTGCTGCA 
    CTTAATAATC ATGTTGAAGT AGCGGAGTTA TTGGTTCATC AGGGAAACGC AAACCTGGAC 
    ATTCAGAATG TAAACCAGCA GACTGCTCTT CACTTGGCTG TGGAACGTCA GCATACACAA 
    ATTGTCAGAC TGTTGGTTCG TGCAGAAGCT AAACTGGACA TACAAGATAA AGATGGGGAT 
    ACACCATTAC ATGAAGCCCT TCGGCACCAT ACTTTGTCAC AGTTGCGGCA GCTTCAAGAC 
    ATGCAGGATG TTGGCAAGGT TGATACTACC TGGGAGCCAT CAAAGAACAC ATTAATAATG 
    GGACTTGGTA CTCAAGGAGC TGAGAAGAAA AGTGCAGCCT CCATTGCCTG CTTTTTGGCT 
    GCCAATGGAG CTGACCTTAC AATCCGTAAC AAAAAAGGAC AGTCCCCTCT TGACCTCTGT 
    CCAGACCCCA ACCTTTGCAA AGCTTTGGCT AAATGTCACA AGGAGAAATC CAGTGGTCAA 
    GTAGGATCCC ATAGTCCCTC TATGATAAGC AATGATTCTG AGACATTAGA AGAATGTATG 
    GTGTGTTCAG ATTTAAAGAG AGATACTCTT TTTGGGCCAT GTGGACACAT TGCTACATGT 
    TCTTTATGTT CTCCACGTGT TAAGAAATGC CTCATATGCA AAGAACAAGT CCAGTCCAGG 
    ACAAAGATAG AAGAATGTGT GGTGTGTTCA GATAAGAAGG CCGCGGTGCT CTTCCAGCCT 
    TGTGGCCATA TGTGTGCTTG TGAAAACTGT GCAAGTCTAA TGAAGAAATG TGTTCAGTGC 
    CGTGCTGTGG TTGAGCGAAG AGTGCCTTTT GTTATGTGTT GCAGTGGCAA AGGTACAGAG 
    GATGTCACAG ATGATATAGC CGGCGGAAAC ATACAAGCTT TACAGAAGGA CAAGGACAAT 
    ACCAATGTCA ATGCAGATGT GCAGAAGCTG CAGCAGCAGT TACAGGACAT AAAGGAACAG 
    ACAATGTGCC CTGTTTGTTT GGATCGGTTG AAGAATATGA TCTTCATGTG TGGACATGGA 
    ACTTGCCAGC TGTGTGGAGA TCGAATGAGT GAATGCCCCA TTTGTCGGAA GGCCATAGAG 
    AGAAGAATAT TATTGTACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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