phldb1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

  • Gene

  • Clones

  • gRNAs

The following phldb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the phldb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa32409 XM_012966171.3
Latest version!
Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa32410 XM_012966172.3
Latest version!
Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa32411 XM_012966173.3
Latest version!
Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa32412 XM_012966174.2
Latest version!
Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa32413 XM_012966175.3
Latest version!
Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa32414 XM_012966176.3
Latest version!
Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa32415 XM_012966177.3
Latest version!
Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa32416 XM_012966178.3
Latest version!
Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa32417 XM_012966179.3
Latest version!
Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa32418 XM_012966180.3
Latest version!
Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa32419 XM_012966182.3
Latest version!
Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa32420 XM_012966183.3
Latest version!
Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa32421 XM_012966184.3
Latest version!
Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa32422 XM_012966185.3
Latest version!
Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa32424 XM_012966188.3
Latest version!
Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa45339 XM_018095290.1
Latest version!
Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OXa61273 XM_031904870.1
Latest version!
Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa61274 XM_031904871.1
Latest version!
Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa61275 XM_031904872.1
Latest version!
Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa61276 XM_031904873.1
Latest version!
Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa32404 XM_012966165.3
Latest version!
Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa32405 XM_012966167.3
Latest version!
Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa32406 XM_012966168.3
Latest version!
Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa32407 XM_012966169.3
Latest version!
Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa32408 XM_012966170.3
Latest version!
Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa61272 XM_031904869.1
Latest version!
Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa02934 NM_001130332.1
Latest version!
Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa32423 XM_012966186.2
Latest version!
Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OXa32416 XM_012966178.2 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa32412 XM_012966174.1 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa32405 XM_012966167.2 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa32406 XM_012966168.2 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa32404 XM_012966165.2 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa32410 XM_012966172.2 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa32415 XM_012966177.2 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa32420 XM_012966183.2 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa32407 XM_012966169.2 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa32408 XM_012966170.2 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa32414 XM_012966176.2 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa32418 XM_012966180.2 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa32411 XM_012966173.2 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa32419 XM_012966182.2 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa32424 XM_012966188.2 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa32409 XM_012966171.2 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa32413 XM_012966175.2 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa32417 XM_012966179.2 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa32421 XM_012966184.2 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa32422 XM_012966185.2 Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa32409
    Clone ID Related Accession (Same CDS sequence) XM_012966171.2 , XM_012966171.3
    Accession Version XM_012966171.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4491bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pleckstrin homology-like domain family B member 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966171.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA
    GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG
    CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT
    CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA
    GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA
    ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT
    GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA
    ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA
    GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA
    TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA
    GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT
    CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC
    TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA
    CAAAAAGACA GTCTGAGCAC AGAAGATTCT CTTCCATCTG AAGTTGAAGG TCAACAAGTG
    ATAGAGGAGC AGCGCAGACG TCTTGCAGAG TTGAAACAGA GAGCTGCAGT TGAGGCACAA
    TCTCAGTGGG AATCTCTCCA TGGAGCCCCT AATCAAGGGT ACACGCCTAT AATTCACCAC
    TCCATTCTAC ACCATCAGCG GCCTCTCCAT TCTCGTGAAG ATGACCCTAC CCATGGATTT
    GACACTCTCA GTCTGGAGAG TTCTGATAGT TTGGACACTA GTGTTTCTAC AGGAAACTCT
    GCATGTTCCC CTGATAACAT GTCAAGTGCT AGTGGGTTAG ACATGCTAAA GATAGAGGAA
    ATGGAAAGAA TGCTTCTAGA AGCTCATGCA GAGCGTGCCA GGCTTGTAGA ATCCAGGGAG
    CGTGAGGTGG AGGCACGCAG AAAAGCACTA GAAGATGAGA GGCGAAGGAG AGAGGAGCTG
    GAAAGGCGTC TTCAAGATGA GACAGCTCAC AGGCAAAGAC TAGTAGAGAA GGAGGTCAAG
    ATGAGGGAGA AGCATTTTTC ACAGGCTCGT CCTCTTACAC GCTATCTGCC AATCCGGAAG
    GAAGACTTTG ATCTCCGAGC CCATGTTGAG TCCTCAGGCC ATGCAGTAGA TGCCTGCCCT
    CACATCATTT TAAGTGAGAA GATGTGTCGT GGCTTCCTGA CAAAAATGGG TGGAAAGATA
    AAATCATGGA AGAAGCGCTG GTTTGTTTTT GATCGCCTGA AACGGACCCT GTCCTATTAT
    GTAGATAAAC ATGAGGCAAA GCTGAAAGGA GTTATATATT TCCAGGCCAT AGAAGAGGTT
    TATTATGACC ACCTCAGGAG TGCAGCCAAG AGTCCTAACC CAGCTCTAAC ATTCTGTGTC
    AAGACTCACG ATCGCCTGTA TTACATGGTA GCTCCTTCCC CAGAGGCTAT GCGGATATGG
    ATGGATGTCA TTGTAACTGG GGCAGAGGGT TACACTCAGT TCATGAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821625.1
    CDS35..4525
    Translation

    Target ORF information:

    RefSeq Version XM_012966171.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966171.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA
    GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG
    CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT
    CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA
    GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA
    ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT
    GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA
    ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA
    GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA
    TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA
    GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT
    CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC
    TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA
    CAAAAAGACA GTCTGAGCAC AGAAGATTCT CTTCCATCTG AAGTTGAAGG TCAACAAGTG
    ATAGAGGAGC AGCGCAGACG TCTTGCAGAG TTGAAACAGA GAGCTGCAGT TGAGGCACAA
    TCTCAGTGGG AATCTCTCCA TGGAGCCCCT AATCAAGGGT ACACGCCTAT AATTCACCAC
    TCCATTCTAC ACCATCAGCG GCCTCTCCAT TCTCGTGAAG ATGACCCTAC CCATGGATTT
    GACACTCTCA GTCTGGAGAG TTCTGATAGT TTGGACACTA GTGTTTCTAC AGGAAACTCT
    GCATGTTCCC CTGATAACAT GTCAAGTGCT AGTGGGTTAG ACATGCTAAA GATAGAGGAA
    ATGGAAAGAA TGCTTCTAGA AGCTCATGCA GAGCGTGCCA GGCTTGTAGA ATCCAGGGAG
    CGTGAGGTGG AGGCACGCAG AAAAGCACTA GAAGATGAGA GGCGAAGGAG AGAGGAGCTG
    GAAAGGCGTC TTCAAGATGA GACAGCTCAC AGGCAAAGAC TAGTAGAGAA GGAGGTCAAG
    ATGAGGGAGA AGCATTTTTC ACAGGCTCGT CCTCTTACAC GCTATCTGCC AATCCGGAAG
    GAAGACTTTG ATCTCCGAGC CCATGTTGAG TCCTCAGGCC ATGCAGTAGA TGCCTGCCCT
    CACATCATTT TAAGTGAGAA GATGTGTCGT GGCTTCCTGA CAAAAATGGG TGGAAAGATA
    AAATCATGGA AGAAGCGCTG GTTTGTTTTT GATCGCCTGA AACGGACCCT GTCCTATTAT
    GTAGATAAAC ATGAGGCAAA GCTGAAAGGA GTTATATATT TCCAGGCCAT AGAAGAGGTT
    TATTATGACC ACCTCAGGAG TGCAGCCAAG AGTCCTAACC CAGCTCTAAC ATTCTGTGTC
    AAGACTCACG ATCGCCTGTA TTACATGGTA GCTCCTTCCC CAGAGGCTAT GCGGATATGG
    ATGGATGTCA TTGTAACTGG GGCAGAGGGT TACACTCAGT TCATGAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32410
    Clone ID Related Accession (Same CDS sequence) XM_012966172.3 , XM_012966172.2
    Accession Version XM_012966172.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4458bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pleckstrin homology-like domain family B member 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966172.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA
    GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG
    CAAGAGTACA TCACTTTAGA TCAGCTGCAG GATATTCTGG AAAGCATGCC CACAGGTTCT
    GCTCCAACTG CTGTGGCAAT GGCTGGCACT GATATCAACT CTGCCTCTTG TGCTCCTTCA
    CCTGACGACC CTCCTCCTGT ACATCTCTCG TCTTCATCTT CTTCCTCTCC CTCCATTTCT
    CTGGAGGTGA ATTCCCCAAC AACAGAAGGT CACTCTGCCC CCCTAGCACC TGATTTGGAT
    GCCTATTACC AAGATTTAGT AACTGCACCT TCTGCCTCTT CTTCTCCATC TTCTTCCCCT
    CCTCCACTCC CTGCTAAATC CCACTCATCA CGTAAGGCTT TGCAGATTTC TCGCTCTCGG
    ACAGAAATTG AGGGTGGAGG CACTCTTCCA CGGATGAAAA CAGTCTCCCC CGCTTCTTCT
    CACTTTTCAG TGGCCACTCT GGGCCGTAGT GTTTCCCCAA AGAATGCCCA TCTGACTCAG
    AATGGCACAG GCAGCCTCTC CAGGGGATTG GCAGTAACCA TGCAAGACAT TCAAAGCAAG
    AGACAGCTGG CACTGCAACA AAAAGACAGT CTGAGCACAG AAGATTCTCT TCCATCTGAA
    GTTGAAGGTC AACAAGTGAT AGAGGAGCAG CGCAGACGTC TTGCAGAGTT GAAACAGAGA
    GCTGCAGTTG AGGCACAATC TCAGTGGGAA TCTCTCCATG GAGCCCCTAA TCAAGGGTAC
    ACGCCTATAA TTCACCACTC CATTCTACAC CATCAGCGGC CTCTCCATTC TCGTGAAGAT
    GACCCTACCC ATGGATTTGA CACTCTCAGT CTGGAGAGTT CTGATAGTTT GGACACTAGT
    GTTTCTACAG GAAACTCTGC ATGTTCCCCT GATAACATGT CAAGTGCTAG TGGGTTAGAC
    ATGCTAAAGA TAGAGGAAAT GGAAAGAATG CTTCTAGAAG CTCATGCAGA GCGTGCCAGG
    CTTGTAGAAT CCAGGGAGCG TGAGGTGGAG GCACGCAGAA AAGCACTAGA AGATGAGAGG
    CGAAGGAGAG AGGAGCTGGA AAGGCGTCTT CAAGATGAGA CAGCTCACAG GCAAAGACTA
    GTAGAGAAGG AGGTCAAGAT GAGGGAGAAG CATTTTTCAC AGGCTCGTCC TCTTACACGC
    TATCTGCCAA TCCGGAAGGA AGACTTTGAT CTCCGAGCCC ATGTTGAGTC CTCAGGCCAT
    GCAGTAGATG CCTGCCCTCA CATCATTTTA AGTGAGAAGA TGTGTCGTGG CTTCCTGACA
    AAAATGGGTG GAAAGATAAA ATCATGGAAG AAGCGCTGGT TTGTTTTTGA TCGCCTGAAA
    CGGACCCTGT CCTATTATGT AGATAAACAT GAGGCAAAGC TGAAAGGAGT TATATATTTC
    CAGGCCATAG AAGAGGTTTA TTATGACCAC CTCAGGAGTG CAGCCAAGAA AGGACTTTTT
    AACCTCAGCC TGGCCACTCA GATATCCGAT TTTCTTTCTG TGCCCCTGTG GAAGGAGAGT
    CCTAACCCAG CTCTAACATT CTGTGTCAAG ACTCACGATC GCCTGTATTA CATGGTAGCT
    CCTTCCCCAG AGGCTATGCG GATATGGATG GATGTCATTG TAACTGGGGC AGAGGGTTAC
    ACTCAGTTCA TGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821626.1
    CDS35..4492
    Translation

    Target ORF information:

    RefSeq Version XM_012966172.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966172.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA
    GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG
    CAAGAGTACA TCACTTTAGA TCAGCTGCAG GATATTCTGG AAAGCATGCC CACAGGTTCT
    GCTCCAACTG CTGTGGCAAT GGCTGGCACT GATATCAACT CTGCCTCTTG TGCTCCTTCA
    CCTGACGACC CTCCTCCTGT ACATCTCTCG TCTTCATCTT CTTCCTCTCC CTCCATTTCT
    CTGGAGGTGA ATTCCCCAAC AACAGAAGGT CACTCTGCCC CCCTAGCACC TGATTTGGAT
    GCCTATTACC AAGATTTAGT AACTGCACCT TCTGCCTCTT CTTCTCCATC TTCTTCCCCT
    CCTCCACTCC CTGCTAAATC CCACTCATCA CGTAAGGCTT TGCAGATTTC TCGCTCTCGG
    ACAGAAATTG AGGGTGGAGG CACTCTTCCA CGGATGAAAA CAGTCTCCCC CGCTTCTTCT
    CACTTTTCAG TGGCCACTCT GGGCCGTAGT GTTTCCCCAA AGAATGCCCA TCTGACTCAG
    AATGGCACAG GCAGCCTCTC CAGGGGATTG GCAGTAACCA TGCAAGACAT TCAAAGCAAG
    AGACAGCTGG CACTGCAACA AAAAGACAGT CTGAGCACAG AAGATTCTCT TCCATCTGAA
    GTTGAAGGTC AACAAGTGAT AGAGGAGCAG CGCAGACGTC TTGCAGAGTT GAAACAGAGA
    GCTGCAGTTG AGGCACAATC TCAGTGGGAA TCTCTCCATG GAGCCCCTAA TCAAGGGTAC
    ACGCCTATAA TTCACCACTC CATTCTACAC CATCAGCGGC CTCTCCATTC TCGTGAAGAT
    GACCCTACCC ATGGATTTGA CACTCTCAGT CTGGAGAGTT CTGATAGTTT GGACACTAGT
    GTTTCTACAG GAAACTCTGC ATGTTCCCCT GATAACATGT CAAGTGCTAG TGGGTTAGAC
    ATGCTAAAGA TAGAGGAAAT GGAAAGAATG CTTCTAGAAG CTCATGCAGA GCGTGCCAGG
    CTTGTAGAAT CCAGGGAGCG TGAGGTGGAG GCACGCAGAA AAGCACTAGA AGATGAGAGG
    CGAAGGAGAG AGGAGCTGGA AAGGCGTCTT CAAGATGAGA CAGCTCACAG GCAAAGACTA
    GTAGAGAAGG AGGTCAAGAT GAGGGAGAAG CATTTTTCAC AGGCTCGTCC TCTTACACGC
    TATCTGCCAA TCCGGAAGGA AGACTTTGAT CTCCGAGCCC ATGTTGAGTC CTCAGGCCAT
    GCAGTAGATG CCTGCCCTCA CATCATTTTA AGTGAGAAGA TGTGTCGTGG CTTCCTGACA
    AAAATGGGTG GAAAGATAAA ATCATGGAAG AAGCGCTGGT TTGTTTTTGA TCGCCTGAAA
    CGGACCCTGT CCTATTATGT AGATAAACAT GAGGCAAAGC TGAAAGGAGT TATATATTTC
    CAGGCCATAG AAGAGGTTTA TTATGACCAC CTCAGGAGTG CAGCCAAGAA AGGACTTTTT
    AACCTCAGCC TGGCCACTCA GATATCCGAT TTTCTTTCTG TGCCCCTGTG GAAGGAGAGT
    CCTAACCCAG CTCTAACATT CTGTGTCAAG ACTCACGATC GCCTGTATTA CATGGTAGCT
    CCTTCCCCAG AGGCTATGCG GATATGGATG GATGTCATTG TAACTGGGGC AGAGGGTTAC
    ACTCAGTTCA TGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32411
    Clone ID Related Accession (Same CDS sequence) XM_012966173.3 , XM_012966173.2
    Accession Version XM_012966173.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4449bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pleckstrin homology-like domain family B member 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966173.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA
    GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG
    CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT
    CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA
    GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA
    ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT
    GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA
    ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA
    GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA
    TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA
    GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT
    CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC
    TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA
    CAAAAAGGTC AACAAGTGAT AGAGGAGCAG CGCAGACGTC TTGCAGAGTT GAAACAGAGA
    GCTGCAGTTG AGGCACAATC TCAGTGGGAA TCTCTCCATG GAGCCCCTAA TCAAGGGTAC
    ACGCCTATAA TTCACCACTC CATTCTACAC CATCAGCGGC CTCTCCATTC TCGTGAAGAT
    GACCCTACCC ATGGATTTGA CACTCTCAGT CTGGAGAGTT CTGATAGTTT GGACACTAGT
    GTTTCTACAG GAAACTCTGC ATGTTCCCCT GATAACATGT CAAGTGCTAG TGGGTTAGAC
    ATGCTAAAGA TAGAGGAAAT GGAAAGAATG CTTCTAGAAG CTCATGCAGA GCGTGCCAGG
    CTTGTAGAAT CCAGGGAGCG TGAGGTGGAG GCACGCAGAA AAGCACTAGA AGATGAGAGG
    CGAAGGAGAG AGGAGCTGGA AAGGCGTCTT CAAGATGAGA CAGCTCACAG GCAAAGACTA
    GTAGAGAAGG AGGTCAAGAT GAGGGAGAAG CATTTTTCAC AGGCTCGTCC TCTTACACGC
    TATCTGCCAA TCCGGAAGGA AGACTTTGAT CTCCGAGCCC ATGTTGAGTC CTCAGGCCAT
    GCAGTAGATG CCTGCCCTCA CATCATTTTA AGTGAGAAGA TGTGTCGTGG CTTCCTGACA
    AAAATGGGTG GAAAGATAAA ATCATGGAAG AAGCGCTGGT TTGTTTTTGA TCGCCTGAAA
    CGGACCCTGT CCTATTATGT AGATAAACAT GAGGCAAAGC TGAAAGGAGT TATATATTTC
    CAGGCCATAG AAGAGGTTTA TTATGACCAC CTCAGGAGTG CAGCCAAGAG TCCTAACCCA
    GCTCTAACAT TCTGTGTCAA GACTCACGAT CGCCTGTATT ACATGGTAGC TCCTTCCCCA
    GAGGCTATGC GGATATGGAT GGATGTCATT GTAACTGGGG CAGAGGGTTA CACTCAGTTC
    ATGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821627.1
    CDS35..4483
    Translation

    Target ORF information:

    RefSeq Version XM_012966173.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966173.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA
    GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG
    CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT
    CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA
    GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA
    ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT
    GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA
    ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA
    GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA
    TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA
    GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT
    CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC
    TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA
    CAAAAAGGTC AACAAGTGAT AGAGGAGCAG CGCAGACGTC TTGCAGAGTT GAAACAGAGA
    GCTGCAGTTG AGGCACAATC TCAGTGGGAA TCTCTCCATG GAGCCCCTAA TCAAGGGTAC
    ACGCCTATAA TTCACCACTC CATTCTACAC CATCAGCGGC CTCTCCATTC TCGTGAAGAT
    GACCCTACCC ATGGATTTGA CACTCTCAGT CTGGAGAGTT CTGATAGTTT GGACACTAGT
    GTTTCTACAG GAAACTCTGC ATGTTCCCCT GATAACATGT CAAGTGCTAG TGGGTTAGAC
    ATGCTAAAGA TAGAGGAAAT GGAAAGAATG CTTCTAGAAG CTCATGCAGA GCGTGCCAGG
    CTTGTAGAAT CCAGGGAGCG TGAGGTGGAG GCACGCAGAA AAGCACTAGA AGATGAGAGG
    CGAAGGAGAG AGGAGCTGGA AAGGCGTCTT CAAGATGAGA CAGCTCACAG GCAAAGACTA
    GTAGAGAAGG AGGTCAAGAT GAGGGAGAAG CATTTTTCAC AGGCTCGTCC TCTTACACGC
    TATCTGCCAA TCCGGAAGGA AGACTTTGAT CTCCGAGCCC ATGTTGAGTC CTCAGGCCAT
    GCAGTAGATG CCTGCCCTCA CATCATTTTA AGTGAGAAGA TGTGTCGTGG CTTCCTGACA
    AAAATGGGTG GAAAGATAAA ATCATGGAAG AAGCGCTGGT TTGTTTTTGA TCGCCTGAAA
    CGGACCCTGT CCTATTATGT AGATAAACAT GAGGCAAAGC TGAAAGGAGT TATATATTTC
    CAGGCCATAG AAGAGGTTTA TTATGACCAC CTCAGGAGTG CAGCCAAGAG TCCTAACCCA
    GCTCTAACAT TCTGTGTCAA GACTCACGAT CGCCTGTATT ACATGGTAGC TCCTTCCCCA
    GAGGCTATGC GGATATGGAT GGATGTCATT GTAACTGGGG CAGAGGGTTA CACTCAGTTC
    ATGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32412
    Clone ID Related Accession (Same CDS sequence) XM_012966174.2 , XM_012966174.1
    Accession Version XM_012966174.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4431bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pleckstrin homology-like domain family B member 1 isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966174.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGAAGGCAC TGAGTCATGA TGTGAGCAGC CCCTGCAGAA AAACACACAC AGTTATTCAG 
    AGCACACCAT TGGATCTCAT AGATACAGGA AAAAGTCTTA GGGTGCAGAC AGAAAAACCT
    CATCTTGTCA GCCTGGGAAG TGGACGACTC AGCACTGCAA TCACCCTACT ACCACTGGAA
    GAAGGAAGGA CAACCCTGGG TCATGCATCT AAGGACATTC CCCTGCAGGG ACCTGGCATT
    GCTGCAGAAC ATTGCTACAT TGAAAACATC AGAGGAAACC TCACCCTATA CCCATGTGGA
    AATCCTTGTG CCATCGATGG ACTGCTCATC ACCCAACCGA CACGTCTCAC TCAAGGGTGT
    ATGGTCTGTC TGGGTCAGTC CACCTTCCTG AGATTTAACC ACCCAGCTGA AGCTCACTGG
    ATGAAAACCA TGATTCCTTC CACTGGGAGA AGCCCTGCGC CAGGCTTGTA CCCATCAGAT
    TCTTCTTCCT CCTTGCTAAA TGGTTCTCGT GATGCTGCAT ACCCGAGAAC TGTACCTTCC
    CATACCTCAC TAGTAAGCTC CATTGAAAAA AATCTTCAGG ACATCATGGA CTCGTTGGTT
    TTAGAAGGGC CTGCACCACT GAGCAGGAAG CTTTCCCAGA CCTCACTTAA CCATATCACC
    CCTATGACCA ATGGAGAGAA CAGACGGTAC TTGCTTTCAT CTCCAACAAG TCCACCTTAC
    TCAAACTCAG GTTATGACAA TACCTCTCCA CTGTCTTCTC CTGGAAGTGG AAGTCTCACT
    AGCCCCTCTC CGGGTCAAGA TGCAGGAACG CCAACAGCAC CCCCTGTGGT GCCACTTCGA
    ACTTCTAGCT TTAGCCATTC CTTGCCACGA CCAGTTCCTA CCTATGGAGG AAGCAACCTT
    GACTTGCGTT CTAATCGCAA CACAGGTGGT GGGTCTCTGC TTAGTCCTTC AAGCCCAGCA
    ACAGGAAGCA GGATGGGGGG ACTAAGTGTG CCAAGCAGTC CACTCCTAGG TAGTAAGTTT
    CATCACCAGA TCACAGACCG CCCAACCAGC CCTTTTAGAC AGGTGGTAGG ACCAACACGG
    AGTCTGCCAC CAGAGAGTCC AAGACTTGGG CGGAGGAACT TTGAAAGCAT GAGGGACCTG
    CCTCCACTTA GCCCTTCTCT GCCCCGAAGA AGTTCATCTA GCTCCATTAA ACCTGCTCCA
    GACAGCCCAT CTCGAGTTCC AGACAGCCCC AGCACTTTAG GGCGACGTCG GGTGGGAAGT
    GCTAGCTCTC CTGGCACAGA AAATAATGCA AGTACAAGGC AGATAAGGGA GCGCAGCCCA
    TCTCCCTCCT CTCTTCAAGA GACCTCTCGT CGACTAACTC CAGCTTATAG CCTGGGATCG
    CTTGGGACCC CTTCACAAAG CCCCAGGGCA CAAAGAAAAG GGCCTGTGCT ACGGGAGCGC
    AAGAACAGCA TCAGTGAAAT TTCTGGCAAC CAAGAGGAGC TAATGGACTA CCACAGAAGG
    CAGAGAGAAG AAAGACTTAG AGAGCAGGAA ATGGAAAGGC TGGAGCGGCA GAGACTGGAA
    ACCATTTTAA ACTTGTGTGC CGAATACAGT AAAACAGATG ATACAGCTCC CCCAGCTCGC
    CTAGATGAAA TAAGGAGCTT CCCCACTTTG GGGGGTACTG AGGAAACCAC TGCATCCCTC
    TTCACTCGTA GACCTTCTTG GGAGAGCACC AGAAGTACCA ACCTGGAGGG AACAGGCAAT
    AGGGTGAGAG CACAGGGAGA ACACAATGAG GAAGAGACTC AGAGAGAAGA GAACAGCAGT
    ACAGAGAGTA CCCAACATGA GCAGGAAGAT GCTGTAAGCC CAACTGGGGC TGCAGAATTA
    CTAGCCCTGG AGGAGCAACG CAGGCACACC CTGGGGCATG TGGATCAGCT AAAGAATAGA
    GTGACTGAAT TGGAGGGTCA GTTGCAGGAA AGTGCAAGGG AGGCAGAGAT GGAACGTGCC
    CTTTTGCAAG GGGAACGGGA GGCTGAGCTG TCCCAGTTGC AGCATGAGCA AAGAATGGTC
    CAGCAGCTGC AGGACAGCCT GACCTCTTTA GAAAACAACT TGCAGAAGGA GAAAGACAAG
    GCACGTGAGA AACTGGAGCA GGAGAGGATG GAGCTGCAGA GTATTCAGCT TTCTCACCAC
    GACCTGCAGA GGCAGCTCCA TAACTGCCCT GAGGCTCTGC GGGAGCAGGT GCAGTGCCAG
    CTGACACGGG AGTCTGAACT CCTGGAGACG AAGATGAAGC TGTTTGAGGA TCTGGAGTTC
    CAGCAGCTAG AAAAGGAAAG CCGCCTGGAA GAAGAAAGAG AGACCACAAG TCAACAACTT
    TTACAGAGCA AGGGGGAATG CCAGCGAAGC ATTGCACGCA GGAAGGAACG CCTGGCTGCT
    TTGGAGATGC AAGCCAATCA GATTCGCCTC CAGGCAGCAC AGGATGCAGA CCATCTTGGC
    CAGGAAAGAA GCGCCATACT TTTACAGCTA CACAAGGAGA AAGAGAAGCT GGTTCAGTTG
    GAGAGAAGAT ACCAGGCACT AACAGGACGA CGGGGATTCT CAACTGGATC AGCTGCTCTC
    CGAGAGGATG CTCTTCAAAT CTATGAGACT AGAGACTGCT TACTCGCTCA AGCTGATGTT
    TCTTGCACAC CTGCTATCCC TTCTGCCTGC TCTCACTCCC CTTCCCGGCA GCAAGAGGAA
    TACCTGTGCC TCTCTGATGT TCTCCGCTTG TACAGCAGCT CGCCTCTTGT TCCTGCTTTT
    CCCCTTCTCT CTGCTGCAGA GCCCACCGCC GCCCTTGAGT ACATCACTTT AGATCAGCTG
    CAGGATATTC TGGAAAGCAT GCCCACAGGT TCTGCTCCAA CTGCTGTGGC AATGGCTGGC
    ACTGATATCA ACTCTGCCTC TTGTGCTCCT TCACCTGACG ACCCTCCTCC TGTACATCTC
    TCGTCTTCAT CTTCTTCCTC TCCCTCCATT TCTCTGGAGG TGAATTCCCC AACAACAGAA
    GGTCACTCTG CCCCCCTAGC ACCTGATTTG GATGCCTATT ACCAAGATTT AGTAACTGCA
    CCTTCTGCCT CTTCTTCTCC ATCTTCTTCC CCTCCTCCAC TCCCTGCTAA ATCCCACTCA
    TCACGTAAGG CTTTGCAGAT TTCTCGCTCT CGGACAGAAA TTGAGGGTGG AGGCACTCTT
    CCACGGATGA AAACAGTCTC CCCCGCTTCT TCTCACTTTT CAGTGGCCAC TCTGGGCCGT
    AGTGTTTCCC CAAAGAATGC CCATCTGACT CAGAATGGCA CAGGCAGCCT CTCCAGGGGA
    TTGGCAGTAA CCATGCAAGA CATTCAAAGC AAGAGACAGC TGGCACTGCA ACAAAAAGAC
    AGTCTGAGCA CAGAAGATTC TCTTCCATCT GAAGTTGAAG GTCAACAAGT GATAGAGGAG
    CAGCGCAGAC GTCTTGCAGA GTTGAAACAG AGAGCTGCAG TTGAGGCACA ATCTCAGTGG
    GAATCTCTCC ATGGAGCCCC TAATCAAGGG TACACGCCTA TAATTCACCA CTCCATTCTA
    CACCATCAGC GGCCTCTCCA TTCTCGTGAA GATGACCCTA CCCATGGATT TGACACTCTC
    AGTCTGGAGA GTTCTGATAG TTTGGACACT AGTGTTTCTA CAGGAAACTC TGCATGTTCC
    CCTGATAACA TGTCAAGTGC TAGTGGGTTA GACATGCTAA AGATAGAGGA AATGGAAAGA
    ATGCTTCTAG AAGCTCATGC AGAGCGTGCC AGGCTTGTAG AATCCAGGGA GCGTGAGGTG
    GAGGCACGCA GAAAAGCACT AGAAGATGAG AGGCGAAGGA GAGAGGAGCT GGAAAGGCGT
    CTTCAAGATG AGACAGCTCA CAGGCAAAGA CTAGTAGAGA AGGAGGTCAA GATGAGGGAG
    AAGCATTTTT CACAGGCTCG TCCTCTTACA CGCTATCTGC CAATCCGGAA GGAAGACTTT
    GATCTCCGAG CCCATGTTGA GTCCTCAGGC CATGCAGTAG ATGCCTGCCC TCACATCATT
    TTAAGTGAGA AGATGTGTCG TGGCTTCCTG ACAAAAATGG GTGGAAAGAT AAAATCATGG
    AAGAAGCGCT GGTTTGTTTT TGATCGCCTG AAACGGACCC TGTCCTATTA TGTAGATAAA
    CATGAGGCAA AGCTGAAAGG AGTTATATAT TTCCAGGCCA TAGAAGAGGT TTATTATGAC
    CACCTCAGGA GTGCAGCCAA GAAAGGACTT TTTAACCTCA GCCTGGCCAC TCAGATATCC
    GATTTTCTTT CTGTGCCCCT GTGGAAGGAG AGTCCTAACC CAGCTCTAAC ATTCTGTGTC
    AAGACTCACG ATCGCCTGTA TTACATGGTA GCTCCTTCCC CAGAGGCTAT GCGGATATGG
    ATGGATGTCA TTGTAACTGG GGCAGAGGGT TACACTCAGT TCATGAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821628.1
    CDS195..4625
    Translation

    Target ORF information:

    RefSeq Version XM_012966174.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966174.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGAAGGCAC TGAGTCATGA TGTGAGCAGC CCCTGCAGAA AAACACACAC AGTTATTCAG 
    AGCACACCAT TGGATCTCAT AGATACAGGA AAAAGTCTTA GGGTGCAGAC AGAAAAACCT
    CATCTTGTCA GCCTGGGAAG TGGACGACTC AGCACTGCAA TCACCCTACT ACCACTGGAA
    GAAGGAAGGA CAACCCTGGG TCATGCATCT AAGGACATTC CCCTGCAGGG ACCTGGCATT
    GCTGCAGAAC ATTGCTACAT TGAAAACATC AGAGGAAACC TCACCCTATA CCCATGTGGA
    AATCCTTGTG CCATCGATGG ACTGCTCATC ACCCAACCGA CACGTCTCAC TCAAGGGTGT
    ATGGTCTGTC TGGGTCAGTC CACCTTCCTG AGATTTAACC ACCCAGCTGA AGCTCACTGG
    ATGAAAACCA TGATTCCTTC CACTGGGAGA AGCCCTGCGC CAGGCTTGTA CCCATCAGAT
    TCTTCTTCCT CCTTGCTAAA TGGTTCTCGT GATGCTGCAT ACCCGAGAAC TGTACCTTCC
    CATACCTCAC TAGTAAGCTC CATTGAAAAA AATCTTCAGG ACATCATGGA CTCGTTGGTT
    TTAGAAGGGC CTGCACCACT GAGCAGGAAG CTTTCCCAGA CCTCACTTAA CCATATCACC
    CCTATGACCA ATGGAGAGAA CAGACGGTAC TTGCTTTCAT CTCCAACAAG TCCACCTTAC
    TCAAACTCAG GTTATGACAA TACCTCTCCA CTGTCTTCTC CTGGAAGTGG AAGTCTCACT
    AGCCCCTCTC CGGGTCAAGA TGCAGGAACG CCAACAGCAC CCCCTGTGGT GCCACTTCGA
    ACTTCTAGCT TTAGCCATTC CTTGCCACGA CCAGTTCCTA CCTATGGAGG AAGCAACCTT
    GACTTGCGTT CTAATCGCAA CACAGGTGGT GGGTCTCTGC TTAGTCCTTC AAGCCCAGCA
    ACAGGAAGCA GGATGGGGGG ACTAAGTGTG CCAAGCAGTC CACTCCTAGG TAGTAAGTTT
    CATCACCAGA TCACAGACCG CCCAACCAGC CCTTTTAGAC AGGTGGTAGG ACCAACACGG
    AGTCTGCCAC CAGAGAGTCC AAGACTTGGG CGGAGGAACT TTGAAAGCAT GAGGGACCTG
    CCTCCACTTA GCCCTTCTCT GCCCCGAAGA AGTTCATCTA GCTCCATTAA ACCTGCTCCA
    GACAGCCCAT CTCGAGTTCC AGACAGCCCC AGCACTTTAG GGCGACGTCG GGTGGGAAGT
    GCTAGCTCTC CTGGCACAGA AAATAATGCA AGTACAAGGC AGATAAGGGA GCGCAGCCCA
    TCTCCCTCCT CTCTTCAAGA GACCTCTCGT CGACTAACTC CAGCTTATAG CCTGGGATCG
    CTTGGGACCC CTTCACAAAG CCCCAGGGCA CAAAGAAAAG GGCCTGTGCT ACGGGAGCGC
    AAGAACAGCA TCAGTGAAAT TTCTGGCAAC CAAGAGGAGC TAATGGACTA CCACAGAAGG
    CAGAGAGAAG AAAGACTTAG AGAGCAGGAA ATGGAAAGGC TGGAGCGGCA GAGACTGGAA
    ACCATTTTAA ACTTGTGTGC CGAATACAGT AAAACAGATG ATACAGCTCC CCCAGCTCGC
    CTAGATGAAA TAAGGAGCTT CCCCACTTTG GGGGGTACTG AGGAAACCAC TGCATCCCTC
    TTCACTCGTA GACCTTCTTG GGAGAGCACC AGAAGTACCA ACCTGGAGGG AACAGGCAAT
    AGGGTGAGAG CACAGGGAGA ACACAATGAG GAAGAGACTC AGAGAGAAGA GAACAGCAGT
    ACAGAGAGTA CCCAACATGA GCAGGAAGAT GCTGTAAGCC CAACTGGGGC TGCAGAATTA
    CTAGCCCTGG AGGAGCAACG CAGGCACACC CTGGGGCATG TGGATCAGCT AAAGAATAGA
    GTGACTGAAT TGGAGGGTCA GTTGCAGGAA AGTGCAAGGG AGGCAGAGAT GGAACGTGCC
    CTTTTGCAAG GGGAACGGGA GGCTGAGCTG TCCCAGTTGC AGCATGAGCA AAGAATGGTC
    CAGCAGCTGC AGGACAGCCT GACCTCTTTA GAAAACAACT TGCAGAAGGA GAAAGACAAG
    GCACGTGAGA AACTGGAGCA GGAGAGGATG GAGCTGCAGA GTATTCAGCT TTCTCACCAC
    GACCTGCAGA GGCAGCTCCA TAACTGCCCT GAGGCTCTGC GGGAGCAGGT GCAGTGCCAG
    CTGACACGGG AGTCTGAACT CCTGGAGACG AAGATGAAGC TGTTTGAGGA TCTGGAGTTC
    CAGCAGCTAG AAAAGGAAAG CCGCCTGGAA GAAGAAAGAG AGACCACAAG TCAACAACTT
    TTACAGAGCA AGGGGGAATG CCAGCGAAGC ATTGCACGCA GGAAGGAACG CCTGGCTGCT
    TTGGAGATGC AAGCCAATCA GATTCGCCTC CAGGCAGCAC AGGATGCAGA CCATCTTGGC
    CAGGAAAGAA GCGCCATACT TTTACAGCTA CACAAGGAGA AAGAGAAGCT GGTTCAGTTG
    GAGAGAAGAT ACCAGGCACT AACAGGACGA CGGGGATTCT CAACTGGATC AGCTGCTCTC
    CGAGAGGATG CTCTTCAAAT CTATGAGACT AGAGACTGCT TACTCGCTCA AGCTGATGTT
    TCTTGCACAC CTGCTATCCC TTCTGCCTGC TCTCACTCCC CTTCCCGGCA GCAAGAGGAA
    TACCTGTGCC TCTCTGATGT TCTCCGCTTG TACAGCAGCT CGCCTCTTGT TCCTGCTTTT
    CCCCTTCTCT CTGCTGCAGA GCCCACCGCC GCCCTTGAGT ACATCACTTT AGATCAGCTG
    CAGGATATTC TGGAAAGCAT GCCCACAGGT TCTGCTCCAA CTGCTGTGGC AATGGCTGGC
    ACTGATATCA ACTCTGCCTC TTGTGCTCCT TCACCTGACG ACCCTCCTCC TGTACATCTC
    TCGTCTTCAT CTTCTTCCTC TCCCTCCATT TCTCTGGAGG TGAATTCCCC AACAACAGAA
    GGTCACTCTG CCCCCCTAGC ACCTGATTTG GATGCCTATT ACCAAGATTT AGTAACTGCA
    CCTTCTGCCT CTTCTTCTCC ATCTTCTTCC CCTCCTCCAC TCCCTGCTAA ATCCCACTCA
    TCACGTAAGG CTTTGCAGAT TTCTCGCTCT CGGACAGAAA TTGAGGGTGG AGGCACTCTT
    CCACGGATGA AAACAGTCTC CCCCGCTTCT TCTCACTTTT CAGTGGCCAC TCTGGGCCGT
    AGTGTTTCCC CAAAGAATGC CCATCTGACT CAGAATGGCA CAGGCAGCCT CTCCAGGGGA
    TTGGCAGTAA CCATGCAAGA CATTCAAAGC AAGAGACAGC TGGCACTGCA ACAAAAAGAC
    AGTCTGAGCA CAGAAGATTC TCTTCCATCT GAAGTTGAAG GTCAACAAGT GATAGAGGAG
    CAGCGCAGAC GTCTTGCAGA GTTGAAACAG AGAGCTGCAG TTGAGGCACA ATCTCAGTGG
    GAATCTCTCC ATGGAGCCCC TAATCAAGGG TACACGCCTA TAATTCACCA CTCCATTCTA
    CACCATCAGC GGCCTCTCCA TTCTCGTGAA GATGACCCTA CCCATGGATT TGACACTCTC
    AGTCTGGAGA GTTCTGATAG TTTGGACACT AGTGTTTCTA CAGGAAACTC TGCATGTTCC
    CCTGATAACA TGTCAAGTGC TAGTGGGTTA GACATGCTAA AGATAGAGGA AATGGAAAGA
    ATGCTTCTAG AAGCTCATGC AGAGCGTGCC AGGCTTGTAG AATCCAGGGA GCGTGAGGTG
    GAGGCACGCA GAAAAGCACT AGAAGATGAG AGGCGAAGGA GAGAGGAGCT GGAAAGGCGT
    CTTCAAGATG AGACAGCTCA CAGGCAAAGA CTAGTAGAGA AGGAGGTCAA GATGAGGGAG
    AAGCATTTTT CACAGGCTCG TCCTCTTACA CGCTATCTGC CAATCCGGAA GGAAGACTTT
    GATCTCCGAG CCCATGTTGA GTCCTCAGGC CATGCAGTAG ATGCCTGCCC TCACATCATT
    TTAAGTGAGA AGATGTGTCG TGGCTTCCTG ACAAAAATGG GTGGAAAGAT AAAATCATGG
    AAGAAGCGCT GGTTTGTTTT TGATCGCCTG AAACGGACCC TGTCCTATTA TGTAGATAAA
    CATGAGGCAA AGCTGAAAGG AGTTATATAT TTCCAGGCCA TAGAAGAGGT TTATTATGAC
    CACCTCAGGA GTGCAGCCAA GAAAGGACTT TTTAACCTCA GCCTGGCCAC TCAGATATCC
    GATTTTCTTT CTGTGCCCCT GTGGAAGGAG AGTCCTAACC CAGCTCTAAC ATTCTGTGTC
    AAGACTCACG ATCGCCTGTA TTACATGGTA GCTCCTTCCC CAGAGGCTAT GCGGATATGG
    ATGGATGTCA TTGTAACTGG GGCAGAGGGT TACACTCAGT TCATGAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32413
    Clone ID Related Accession (Same CDS sequence) XM_012966175.2 , XM_012966175.3
    Accession Version XM_012966175.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4431bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pleckstrin homology-like domain family B member 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966175.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG AGTCTGAACT CCTGGAGACG AAGATGAAGC TGTTTGAGGA TCTGGAGTTC
    CAGCAGCTAG AAAAGGAAAG CCGCCTGGAA GAAGAAAGAG AGACCACAAG TCAACAACTT
    TTACAGAGCA AGGGGGAATG CCAGCGAAGC ATTGCACGCA GGAAGGAACG CCTGGCTGCT
    TTGGAGATGC AAGCCAATCA GATTCGCCTC CAGGCAGCAC AGGATGCAGA CCATCTTGGC
    CAGGAAAGAA GCGCCATACT TTTACAGCTA CACAAGGAGA AAGAGAAGCT GGTTCAGTTG
    GAGAGAAGAT ACCAGGCACT AACAGGACGA CGGGGATTCT CAACTGGATC AGCTGCTCTC
    CGAGAGGATG CTCTTCAAAT CTATGAGACT AGAGACTGCT TACTCGCTCA AGCTGATGTT
    TCTTGCACAC CTGCTATCCC TTCTGCCTGC TCTCACTCCC CTTCCCGGCA GCAAGAGGAA
    TACCTGTGCC TCTCTGATGT TCTCCGCTTG TACAGCAGCT CGCCTCTTGT TCCTGCTTTT
    CCCCTTCTCT CTGCTGCAGA GCCCACCGCC GCCCTTGAGT ACATCACTTT AGATCAGCTG
    CAGGATATTC TGGAAAGCAT GCCCACAGGT TCTGCTCCAA CTGCTGTGGC AATGGCTGGC
    ACTGATATCA ACTCTGCCTC TTGTGCTCCT TCACCTGACG ACCCTCCTCC TGTACATCTC
    TCGTCTTCAT CTTCTTCCTC TCCCTCCATT TCTCTGGAGG TGAATTCCCC AACAACAGAA
    GGTCACTCTG CCCCCCTAGC ACCTGATTTG GATGCCTATT ACCAAGATTT AGTAACTGCA
    CCTTCTGCCT CTTCTTCTCC ATCTTCTTCC CCTCCTCCAC TCCCTGCTAA ATCCCACTCA
    TCACGTAAGG CTTTGCAGAT TTCTCGCTCT CGGACAGAAA TTGAGGGTGG AGGCACTCTT
    CCACGGATGA AAACAGTCTC CCCCGCTTCT TCTCACTTTT CAGTGGCCAC TCTGGGCCGT
    AGTGTTTCCC CAAAGAATGC CCATCTGACT CAGAATGGCA CAGGCAGCCT CTCCAGGGGA
    TTGGCAGTAA CCATGCAAGA CATTCAAAGC AAGAGACAGC TGGCACTGCA ACAAAAAGAC
    AGTCTGAGCA CAGAAGATTC TCTTCCATCT GAAGTTGAAG GTCAACAAGT GATAGAGGAG
    CAGCGCAGAC GTCTTGCAGA GTTGAAACAG AGAGCTGCAG TTGAGGCACA ATCTCAGTGG
    GAATCTCTCC ATGGAGCCCC TAATCAAGGG TACACGCCTA TAATTCACCA CTCCATTCTA
    CACCATCAGC GGCCTCTCCA TTCTCGTGAA GATGACCCTA CCCATGGATT TGACACTCTC
    AGTCTGGAGA GTTCTGATAG TTTGGACACT AGTGTTTCTA CAGGAAACTC TGCATGTTCC
    CCTGATAACA TGTCAAGTGC TAGTGGGTTA GACATGCTAA AGATAGAGGA AATGGAAAGA
    ATGCTTCTAG AAGCTCATGC AGAGCGTGCC AGGCTTGTAG AATCCAGGGA GCGTGAGGTG
    GAGGCACGCA GAAAAGCACT AGAAGATGAG AGGCGAAGGA GAGAGGAGCT GGAAAGGCGT
    CTTCAAGATG AGACAGCTCA CAGGCAAAGA CTAGTAGAGA AGGAGGTCAA GATGAGGGAG
    AAGCATTTTT CACAGGCTCG TCCTCTTACA CGCTATCTGC CAATCCGGAA GGAAGACTTT
    GATCTCCGAG CCCATGTTGA GTCCTCAGGC CATGCAGTAG ATGCCTGCCC TCACATCATT
    TTAAGTGAGA AGATGTGTCG TGGCTTCCTG ACAAAAATGG GTGGAAAGAT AAAATCATGG
    AAGAAGCGCT GGTTTGTTTT TGATCGCCTG AAACGGACCC TGTCCTATTA TGTAGATAAA
    CATGAGGCAA AGCTGAAAGG AGTTATATAT TTCCAGGCCA TAGAAGAGGT TTATTATGAC
    CACCTCAGGA GTGCAGCCAA GAAAGGACTT TTTAACCTCA GCCTGGCCAC TCAGATATCC
    GATTTTCTTT CTGTGCCCCT GTGGAAGGAG AGTCCTAACC CAGCTCTAAC ATTCTGTGTC
    AAGACTCACG ATCGCCTGTA TTACATGGTA GCTCCTTCCC CAGAGGCTAT GCGGATATGG
    ATGGATGTCA TTGTAACTGG GGCAGAGGGT TACACTCAGT TCATGAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821629.1
    CDS35..4465
    Translation

    Target ORF information:

    RefSeq Version XM_012966175.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966175.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG AGTCTGAACT CCTGGAGACG AAGATGAAGC TGTTTGAGGA TCTGGAGTTC
    CAGCAGCTAG AAAAGGAAAG CCGCCTGGAA GAAGAAAGAG AGACCACAAG TCAACAACTT
    TTACAGAGCA AGGGGGAATG CCAGCGAAGC ATTGCACGCA GGAAGGAACG CCTGGCTGCT
    TTGGAGATGC AAGCCAATCA GATTCGCCTC CAGGCAGCAC AGGATGCAGA CCATCTTGGC
    CAGGAAAGAA GCGCCATACT TTTACAGCTA CACAAGGAGA AAGAGAAGCT GGTTCAGTTG
    GAGAGAAGAT ACCAGGCACT AACAGGACGA CGGGGATTCT CAACTGGATC AGCTGCTCTC
    CGAGAGGATG CTCTTCAAAT CTATGAGACT AGAGACTGCT TACTCGCTCA AGCTGATGTT
    TCTTGCACAC CTGCTATCCC TTCTGCCTGC TCTCACTCCC CTTCCCGGCA GCAAGAGGAA
    TACCTGTGCC TCTCTGATGT TCTCCGCTTG TACAGCAGCT CGCCTCTTGT TCCTGCTTTT
    CCCCTTCTCT CTGCTGCAGA GCCCACCGCC GCCCTTGAGT ACATCACTTT AGATCAGCTG
    CAGGATATTC TGGAAAGCAT GCCCACAGGT TCTGCTCCAA CTGCTGTGGC AATGGCTGGC
    ACTGATATCA ACTCTGCCTC TTGTGCTCCT TCACCTGACG ACCCTCCTCC TGTACATCTC
    TCGTCTTCAT CTTCTTCCTC TCCCTCCATT TCTCTGGAGG TGAATTCCCC AACAACAGAA
    GGTCACTCTG CCCCCCTAGC ACCTGATTTG GATGCCTATT ACCAAGATTT AGTAACTGCA
    CCTTCTGCCT CTTCTTCTCC ATCTTCTTCC CCTCCTCCAC TCCCTGCTAA ATCCCACTCA
    TCACGTAAGG CTTTGCAGAT TTCTCGCTCT CGGACAGAAA TTGAGGGTGG AGGCACTCTT
    CCACGGATGA AAACAGTCTC CCCCGCTTCT TCTCACTTTT CAGTGGCCAC TCTGGGCCGT
    AGTGTTTCCC CAAAGAATGC CCATCTGACT CAGAATGGCA CAGGCAGCCT CTCCAGGGGA
    TTGGCAGTAA CCATGCAAGA CATTCAAAGC AAGAGACAGC TGGCACTGCA ACAAAAAGAC
    AGTCTGAGCA CAGAAGATTC TCTTCCATCT GAAGTTGAAG GTCAACAAGT GATAGAGGAG
    CAGCGCAGAC GTCTTGCAGA GTTGAAACAG AGAGCTGCAG TTGAGGCACA ATCTCAGTGG
    GAATCTCTCC ATGGAGCCCC TAATCAAGGG TACACGCCTA TAATTCACCA CTCCATTCTA
    CACCATCAGC GGCCTCTCCA TTCTCGTGAA GATGACCCTA CCCATGGATT TGACACTCTC
    AGTCTGGAGA GTTCTGATAG TTTGGACACT AGTGTTTCTA CAGGAAACTC TGCATGTTCC
    CCTGATAACA TGTCAAGTGC TAGTGGGTTA GACATGCTAA AGATAGAGGA AATGGAAAGA
    ATGCTTCTAG AAGCTCATGC AGAGCGTGCC AGGCTTGTAG AATCCAGGGA GCGTGAGGTG
    GAGGCACGCA GAAAAGCACT AGAAGATGAG AGGCGAAGGA GAGAGGAGCT GGAAAGGCGT
    CTTCAAGATG AGACAGCTCA CAGGCAAAGA CTAGTAGAGA AGGAGGTCAA GATGAGGGAG
    AAGCATTTTT CACAGGCTCG TCCTCTTACA CGCTATCTGC CAATCCGGAA GGAAGACTTT
    GATCTCCGAG CCCATGTTGA GTCCTCAGGC CATGCAGTAG ATGCCTGCCC TCACATCATT
    TTAAGTGAGA AGATGTGTCG TGGCTTCCTG ACAAAAATGG GTGGAAAGAT AAAATCATGG
    AAGAAGCGCT GGTTTGTTTT TGATCGCCTG AAACGGACCC TGTCCTATTA TGTAGATAAA
    CATGAGGCAA AGCTGAAAGG AGTTATATAT TTCCAGGCCA TAGAAGAGGT TTATTATGAC
    CACCTCAGGA GTGCAGCCAA GAAAGGACTT TTTAACCTCA GCCTGGCCAC TCAGATATCC
    GATTTTCTTT CTGTGCCCCT GTGGAAGGAG AGTCCTAACC CAGCTCTAAC ATTCTGTGTC
    AAGACTCACG ATCGCCTGTA TTACATGGTA GCTCCTTCCC CAGAGGCTAT GCGGATATGG
    ATGGATGTCA TTGTAACTGG GGCAGAGGGT TACACTCAGT TCATGAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32414
    Clone ID Related Accession (Same CDS sequence) XM_012966176.2 , XM_012966176.3
    Accession Version XM_012966176.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4401bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pleckstrin homology-like domain family B member 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966176.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA
    GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG
    CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT
    CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA
    GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA
    ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT
    GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGAT TTCTCGCTCT
    CGGACAGAAA TTGAGGGTGG AGGCACTCTT CCACGGATGA AAACAGTCTC CCCCGCTTCT
    TCTCACTTTT CAGTGGCCAC TCTGGGCCGT AGTGTTTCCC CAAAGAATGC CCATCTGACT
    CAGAATGGCA CAGGCAGCCT CTCCAGGGGA TTGGCAGTAA CCATGCAAGA CATTCAAAGC
    AAGAGACAGC TGGCACTGCA ACAAAAAGAC AGTCTGAGCA CAGAAGATTC TCTTCCATCT
    GAAGTTGAAG GTCAACAAGT GATAGAGGAG CAGCGCAGAC GTCTTGCAGA GTTGAAACAG
    AGAGCTGCAG TTGAGGCACA ATCTCAGTGG GAATCTCTCC ATGGAGCCCC TAATCAAGGG
    TACACGCCTA TAATTCACCA CTCCATTCTA CACCATCAGC GGCCTCTCCA TTCTCGTGAA
    GATGACCCTA CCCATGGATT TGACACTCTC AGTCTGGAGA GTTCTGATAG TTTGGACACT
    AGTGTTTCTA CAGGAAACTC TGCATGTTCC CCTGATAACA TGTCAAGTGC TAGTGGGTTA
    GACATGCTAA AGATAGAGGA AATGGAAAGA ATGCTTCTAG AAGCTCATGC AGAGCGTGCC
    AGGCTTGTAG AATCCAGGGA GCGTGAGGTG GAGGCACGCA GAAAAGCACT AGAAGATGAG
    AGGCGAAGGA GAGAGGAGCT GGAAAGGCGT CTTCAAGATG AGACAGCTCA CAGGCAAAGA
    CTAGTAGAGA AGGAGGTCAA GATGAGGGAG AAGCATTTTT CACAGGCTCG TCCTCTTACA
    CGCTATCTGC CAATCCGGAA GGAAGACTTT GATCTCCGAG CCCATGTTGA GTCCTCAGGC
    CATGCAGTAG ATGCCTGCCC TCACATCATT TTAAGTGAGA AGATGTGTCG TGGCTTCCTG
    ACAAAAATGG GTGGAAAGAT AAAATCATGG AAGAAGCGCT GGTTTGTTTT TGATCGCCTG
    AAACGGACCC TGTCCTATTA TGTAGATAAA CATGAGGCAA AGCTGAAAGG AGTTATATAT
    TTCCAGGCCA TAGAAGAGGT TTATTATGAC CACCTCAGGA GTGCAGCCAA GAAAGGACTT
    TTTAACCTCA GCCTGGCCAC TCAGATATCC GATTTTCTTT CTGTGCCCCT GTGGAAGGAG
    AGTCCTAACC CAGCTCTAAC ATTCTGTGTC AAGACTCACG ATCGCCTGTA TTACATGGTA
    GCTCCTTCCC CAGAGGCTAT GCGGATATGG ATGGATGTCA TTGTAACTGG GGCAGAGGGT
    TACACTCAGT TCATGAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821630.1
    CDS35..4435
    Translation

    Target ORF information:

    RefSeq Version XM_012966176.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966176.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA
    GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG
    CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT
    CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA
    GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA
    ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT
    GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGAT TTCTCGCTCT
    CGGACAGAAA TTGAGGGTGG AGGCACTCTT CCACGGATGA AAACAGTCTC CCCCGCTTCT
    TCTCACTTTT CAGTGGCCAC TCTGGGCCGT AGTGTTTCCC CAAAGAATGC CCATCTGACT
    CAGAATGGCA CAGGCAGCCT CTCCAGGGGA TTGGCAGTAA CCATGCAAGA CATTCAAAGC
    AAGAGACAGC TGGCACTGCA ACAAAAAGAC AGTCTGAGCA CAGAAGATTC TCTTCCATCT
    GAAGTTGAAG GTCAACAAGT GATAGAGGAG CAGCGCAGAC GTCTTGCAGA GTTGAAACAG
    AGAGCTGCAG TTGAGGCACA ATCTCAGTGG GAATCTCTCC ATGGAGCCCC TAATCAAGGG
    TACACGCCTA TAATTCACCA CTCCATTCTA CACCATCAGC GGCCTCTCCA TTCTCGTGAA
    GATGACCCTA CCCATGGATT TGACACTCTC AGTCTGGAGA GTTCTGATAG TTTGGACACT
    AGTGTTTCTA CAGGAAACTC TGCATGTTCC CCTGATAACA TGTCAAGTGC TAGTGGGTTA
    GACATGCTAA AGATAGAGGA AATGGAAAGA ATGCTTCTAG AAGCTCATGC AGAGCGTGCC
    AGGCTTGTAG AATCCAGGGA GCGTGAGGTG GAGGCACGCA GAAAAGCACT AGAAGATGAG
    AGGCGAAGGA GAGAGGAGCT GGAAAGGCGT CTTCAAGATG AGACAGCTCA CAGGCAAAGA
    CTAGTAGAGA AGGAGGTCAA GATGAGGGAG AAGCATTTTT CACAGGCTCG TCCTCTTACA
    CGCTATCTGC CAATCCGGAA GGAAGACTTT GATCTCCGAG CCCATGTTGA GTCCTCAGGC
    CATGCAGTAG ATGCCTGCCC TCACATCATT TTAAGTGAGA AGATGTGTCG TGGCTTCCTG
    ACAAAAATGG GTGGAAAGAT AAAATCATGG AAGAAGCGCT GGTTTGTTTT TGATCGCCTG
    AAACGGACCC TGTCCTATTA TGTAGATAAA CATGAGGCAA AGCTGAAAGG AGTTATATAT
    TTCCAGGCCA TAGAAGAGGT TTATTATGAC CACCTCAGGA GTGCAGCCAA GAAAGGACTT
    TTTAACCTCA GCCTGGCCAC TCAGATATCC GATTTTCTTT CTGTGCCCCT GTGGAAGGAG
    AGTCCTAACC CAGCTCTAAC ATTCTGTGTC AAGACTCACG ATCGCCTGTA TTACATGGTA
    GCTCCTTCCC CAGAGGCTAT GCGGATATGG ATGGATGTCA TTGTAACTGG GGCAGAGGGT
    TACACTCAGT TCATGAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32415
    Clone ID Related Accession (Same CDS sequence) XM_012966177.2 , XM_012966177.3
    Accession Version XM_012966177.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4221bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pleckstrin homology-like domain family B member 1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966177.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA
    GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG
    CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT
    CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGAT TTCTCGCTCT
    CGGACAGAAA TTGAGGGTGG AGGCACTCTT CCACGGATGA AAACAGTCTC CCCCGCTTCT
    TCTCACTTTT CAGTGGCCAC TCTGGGCCGT AGTGTTTCCC CAAAGAATGC CCATCTGACT
    CAGAATGGCA CAGGCAGCCT CTCCAGGGGA TTGGCAGTAA CCATGCAAGA CATTCAAAGC
    AAGAGACAGC TGGCACTGCA ACAAAAAGAC AGTCTGAGCA CAGAAGATTC TCTTCCATCT
    GAAGTTGAAG GTCAACAAGT GATAGAGGAG CAGCGCAGAC GTCTTGCAGA GTTGAAACAG
    AGAGCTGCAG TTGAGGCACA ATCTCAGTGG GAATCTCTCC ATGGAGCCCC TAATCAAGGG
    TACACGCCTA TAATTCACCA CTCCATTCTA CACCATCAGC GGCCTCTCCA TTCTCGTGAA
    GATGACCCTA CCCATGGATT TGACACTCTC AGTCTGGAGA GTTCTGATAG TTTGGACACT
    AGTGTTTCTA CAGGAAACTC TGCATGTTCC CCTGATAACA TGTCAAGTGC TAGTGGGTTA
    GACATGCTAA AGATAGAGGA AATGGAAAGA ATGCTTCTAG AAGCTCATGC AGAGCGTGCC
    AGGCTTGTAG AATCCAGGGA GCGTGAGGTG GAGGCACGCA GAAAAGCACT AGAAGATGAG
    AGGCGAAGGA GAGAGGAGCT GGAAAGGCGT CTTCAAGATG AGACAGCTCA CAGGCAAAGA
    CTAGTAGAGA AGGAGGTCAA GATGAGGGAG AAGCATTTTT CACAGGCTCG TCCTCTTACA
    CGCTATCTGC CAATCCGGAA GGAAGACTTT GATCTCCGAG CCCATGTTGA GTCCTCAGGC
    CATGCAGTAG ATGCCTGCCC TCACATCATT TTAAGTGAGA AGATGTGTCG TGGCTTCCTG
    ACAAAAATGG GTGGAAAGAT AAAATCATGG AAGAAGCGCT GGTTTGTTTT TGATCGCCTG
    AAACGGACCC TGTCCTATTA TGTAGATAAA CATGAGGCAA AGCTGAAAGG AGTTATATAT
    TTCCAGGCCA TAGAAGAGGT TTATTATGAC CACCTCAGGA GTGCAGCCAA GAAAGGACTT
    TTTAACCTCA GCCTGGCCAC TCAGATATCC GATTTTCTTT CTGTGCCCCT GTGGAAGGAG
    AGTCCTAACC CAGCTCTAAC ATTCTGTGTC AAGACTCACG ATCGCCTGTA TTACATGGTA
    GCTCCTTCCC CAGAGGCTAT GCGGATATGG ATGGATGTCA TTGTAACTGG GGCAGAGGGT
    TACACTCAGT TCATGAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821631.1
    CDS35..4255
    Translation

    Target ORF information:

    RefSeq Version XM_012966177.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966177.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA
    GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG
    CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT
    CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGAT TTCTCGCTCT
    CGGACAGAAA TTGAGGGTGG AGGCACTCTT CCACGGATGA AAACAGTCTC CCCCGCTTCT
    TCTCACTTTT CAGTGGCCAC TCTGGGCCGT AGTGTTTCCC CAAAGAATGC CCATCTGACT
    CAGAATGGCA CAGGCAGCCT CTCCAGGGGA TTGGCAGTAA CCATGCAAGA CATTCAAAGC
    AAGAGACAGC TGGCACTGCA ACAAAAAGAC AGTCTGAGCA CAGAAGATTC TCTTCCATCT
    GAAGTTGAAG GTCAACAAGT GATAGAGGAG CAGCGCAGAC GTCTTGCAGA GTTGAAACAG
    AGAGCTGCAG TTGAGGCACA ATCTCAGTGG GAATCTCTCC ATGGAGCCCC TAATCAAGGG
    TACACGCCTA TAATTCACCA CTCCATTCTA CACCATCAGC GGCCTCTCCA TTCTCGTGAA
    GATGACCCTA CCCATGGATT TGACACTCTC AGTCTGGAGA GTTCTGATAG TTTGGACACT
    AGTGTTTCTA CAGGAAACTC TGCATGTTCC CCTGATAACA TGTCAAGTGC TAGTGGGTTA
    GACATGCTAA AGATAGAGGA AATGGAAAGA ATGCTTCTAG AAGCTCATGC AGAGCGTGCC
    AGGCTTGTAG AATCCAGGGA GCGTGAGGTG GAGGCACGCA GAAAAGCACT AGAAGATGAG
    AGGCGAAGGA GAGAGGAGCT GGAAAGGCGT CTTCAAGATG AGACAGCTCA CAGGCAAAGA
    CTAGTAGAGA AGGAGGTCAA GATGAGGGAG AAGCATTTTT CACAGGCTCG TCCTCTTACA
    CGCTATCTGC CAATCCGGAA GGAAGACTTT GATCTCCGAG CCCATGTTGA GTCCTCAGGC
    CATGCAGTAG ATGCCTGCCC TCACATCATT TTAAGTGAGA AGATGTGTCG TGGCTTCCTG
    ACAAAAATGG GTGGAAAGAT AAAATCATGG AAGAAGCGCT GGTTTGTTTT TGATCGCCTG
    AAACGGACCC TGTCCTATTA TGTAGATAAA CATGAGGCAA AGCTGAAAGG AGTTATATAT
    TTCCAGGCCA TAGAAGAGGT TTATTATGAC CACCTCAGGA GTGCAGCCAA GAAAGGACTT
    TTTAACCTCA GCCTGGCCAC TCAGATATCC GATTTTCTTT CTGTGCCCCT GTGGAAGGAG
    AGTCCTAACC CAGCTCTAAC ATTCTGTGTC AAGACTCACG ATCGCCTGTA TTACATGGTA
    GCTCCTTCCC CAGAGGCTAT GCGGATATGG ATGGATGTCA TTGTAACTGG GGCAGAGGGT
    TACACTCAGT TCATGAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32416
    Clone ID Related Accession (Same CDS sequence) XM_012966178.2 , XM_012966178.3
    Accession Version XM_012966178.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4119bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pleckstrin homology-like domain family B member 1 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966178.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA
    GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG
    CAAGAGATTT CTCGCTCTCG GACAGAAATT GAGGGTGGAG GCACTCTTCC ACGGATGAAA
    ACAGTCTCCC CCGCTTCTTC TCACTTTTCA GTGGCCACTC TGGGCCGTAG TGTTTCCCCA
    AAGAATGCCC ATCTGACTCA GAATGGCACA GGCAGCCTCT CCAGGGGATT GGCAGTAACC
    ATGCAAGACA TTCAAAGCAA GAGACAGCTG GCACTGCAAC AAAAAGACAG TCTGAGCACA
    GAAGATTCTC TTCCATCTGA AGTTGAAGGT CAACAAGTGA TAGAGGAGCA GCGCAGACGT
    CTTGCAGAGT TGAAACAGAG AGCTGCAGTT GAGGCACAAT CTCAGTGGGA ATCTCTCCAT
    GGAGCCCCTA ATCAAGGGTA CACGCCTATA ATTCACCACT CCATTCTACA CCATCAGCGG
    CCTCTCCATT CTCGTGAAGA TGACCCTACC CATGGATTTG ACACTCTCAG TCTGGAGAGT
    TCTGATAGTT TGGACACTAG TGTTTCTACA GGAAACTCTG CATGTTCCCC TGATAACATG
    TCAAGTGCTA GTGGGTTAGA CATGCTAAAG ATAGAGGAAA TGGAAAGAAT GCTTCTAGAA
    GCTCATGCAG AGCGTGCCAG GCTTGTAGAA TCCAGGGAGC GTGAGGTGGA GGCACGCAGA
    AAAGCACTAG AAGATGAGAG GCGAAGGAGA GAGGAGCTGG AAAGGCGTCT TCAAGATGAG
    ACAGCTCACA GGCAAAGACT AGTAGAGAAG GAGGTCAAGA TGAGGGAGAA GCATTTTTCA
    CAGGCTCGTC CTCTTACACG CTATCTGCCA ATCCGGAAGG AAGACTTTGA TCTCCGAGCC
    CATGTTGAGT CCTCAGGCCA TGCAGTAGAT GCCTGCCCTC ACATCATTTT AAGTGAGAAG
    ATGTGTCGTG GCTTCCTGAC AAAAATGGGT GGAAAGATAA AATCATGGAA GAAGCGCTGG
    TTTGTTTTTG ATCGCCTGAA ACGGACCCTG TCCTATTATG TAGATAAACA TGAGGCAAAG
    CTGAAAGGAG TTATATATTT CCAGGCCATA GAAGAGGTTT ATTATGACCA CCTCAGGAGT
    GCAGCCAAGA AAGGACTTTT TAACCTCAGC CTGGCCACTC AGATATCCGA TTTTCTTTCT
    GTGCCCCTGT GGAAGGAGAG TCCTAACCCA GCTCTAACAT TCTGTGTCAA GACTCACGAT
    CGCCTGTATT ACATGGTAGC TCCTTCCCCA GAGGCTATGC GGATATGGAT GGATGTCATT
    GTAACTGGGG CAGAGGGTTA CACTCAGTTC ATGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821632.1
    CDS35..4153
    Translation

    Target ORF information:

    RefSeq Version XM_012966178.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966178.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA
    GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG
    CAAGAGATTT CTCGCTCTCG GACAGAAATT GAGGGTGGAG GCACTCTTCC ACGGATGAAA
    ACAGTCTCCC CCGCTTCTTC TCACTTTTCA GTGGCCACTC TGGGCCGTAG TGTTTCCCCA
    AAGAATGCCC ATCTGACTCA GAATGGCACA GGCAGCCTCT CCAGGGGATT GGCAGTAACC
    ATGCAAGACA TTCAAAGCAA GAGACAGCTG GCACTGCAAC AAAAAGACAG TCTGAGCACA
    GAAGATTCTC TTCCATCTGA AGTTGAAGGT CAACAAGTGA TAGAGGAGCA GCGCAGACGT
    CTTGCAGAGT TGAAACAGAG AGCTGCAGTT GAGGCACAAT CTCAGTGGGA ATCTCTCCAT
    GGAGCCCCTA ATCAAGGGTA CACGCCTATA ATTCACCACT CCATTCTACA CCATCAGCGG
    CCTCTCCATT CTCGTGAAGA TGACCCTACC CATGGATTTG ACACTCTCAG TCTGGAGAGT
    TCTGATAGTT TGGACACTAG TGTTTCTACA GGAAACTCTG CATGTTCCCC TGATAACATG
    TCAAGTGCTA GTGGGTTAGA CATGCTAAAG ATAGAGGAAA TGGAAAGAAT GCTTCTAGAA
    GCTCATGCAG AGCGTGCCAG GCTTGTAGAA TCCAGGGAGC GTGAGGTGGA GGCACGCAGA
    AAAGCACTAG AAGATGAGAG GCGAAGGAGA GAGGAGCTGG AAAGGCGTCT TCAAGATGAG
    ACAGCTCACA GGCAAAGACT AGTAGAGAAG GAGGTCAAGA TGAGGGAGAA GCATTTTTCA
    CAGGCTCGTC CTCTTACACG CTATCTGCCA ATCCGGAAGG AAGACTTTGA TCTCCGAGCC
    CATGTTGAGT CCTCAGGCCA TGCAGTAGAT GCCTGCCCTC ACATCATTTT AAGTGAGAAG
    ATGTGTCGTG GCTTCCTGAC AAAAATGGGT GGAAAGATAA AATCATGGAA GAAGCGCTGG
    TTTGTTTTTG ATCGCCTGAA ACGGACCCTG TCCTATTATG TAGATAAACA TGAGGCAAAG
    CTGAAAGGAG TTATATATTT CCAGGCCATA GAAGAGGTTT ATTATGACCA CCTCAGGAGT
    GCAGCCAAGA AAGGACTTTT TAACCTCAGC CTGGCCACTC AGATATCCGA TTTTCTTTCT
    GTGCCCCTGT GGAAGGAGAG TCCTAACCCA GCTCTAACAT TCTGTGTCAA GACTCACGAT
    CGCCTGTATT ACATGGTAGC TCCTTCCCCA GAGGCTATGC GGATATGGAT GGATGTCATT
    GTAACTGGGG CAGAGGGTTA CACTCAGTTC ATGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32417
    Clone ID Related Accession (Same CDS sequence) XM_012966179.2 , XM_012966179.3
    Accession Version XM_012966179.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4077bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pleckstrin homology-like domain family B member 1 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966179.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA
    GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG
    CAAGAGATTT CTCGCTCTCG GACAGAAATT GAGGGTGGAG GCACTCTTCC ACGGATGAAA
    ACAGTCTCCC CCGCTTCTTC TCACTTTTCA GTGGCCACTC TGGGCCGTAG TGTTTCCCCA
    AAGAATGCCC ATCTGACTCA GAATGGCACA GGCAGCCTCT CCAGGGGATT GGCAGTAACC
    ATGCAAGACA TTCAAAGCAA GAGACAGCTG GCACTGCAAC AAAAAGGTCA ACAAGTGATA
    GAGGAGCAGC GCAGACGTCT TGCAGAGTTG AAACAGAGAG CTGCAGTTGA GGCACAATCT
    CAGTGGGAAT CTCTCCATGG AGCCCCTAAT CAAGGGTACA CGCCTATAAT TCACCACTCC
    ATTCTACACC ATCAGCGGCC TCTCCATTCT CGTGAAGATG ACCCTACCCA TGGATTTGAC
    ACTCTCAGTC TGGAGAGTTC TGATAGTTTG GACACTAGTG TTTCTACAGG AAACTCTGCA
    TGTTCCCCTG ATAACATGTC AAGTGCTAGT GGGTTAGACA TGCTAAAGAT AGAGGAAATG
    GAAAGAATGC TTCTAGAAGC TCATGCAGAG CGTGCCAGGC TTGTAGAATC CAGGGAGCGT
    GAGGTGGAGG CACGCAGAAA AGCACTAGAA GATGAGAGGC GAAGGAGAGA GGAGCTGGAA
    AGGCGTCTTC AAGATGAGAC AGCTCACAGG CAAAGACTAG TAGAGAAGGA GGTCAAGATG
    AGGGAGAAGC ATTTTTCACA GGCTCGTCCT CTTACACGCT ATCTGCCAAT CCGGAAGGAA
    GACTTTGATC TCCGAGCCCA TGTTGAGTCC TCAGGCCATG CAGTAGATGC CTGCCCTCAC
    ATCATTTTAA GTGAGAAGAT GTGTCGTGGC TTCCTGACAA AAATGGGTGG AAAGATAAAA
    TCATGGAAGA AGCGCTGGTT TGTTTTTGAT CGCCTGAAAC GGACCCTGTC CTATTATGTA
    GATAAACATG AGGCAAAGCT GAAAGGAGTT ATATATTTCC AGGCCATAGA AGAGGTTTAT
    TATGACCACC TCAGGAGTGC AGCCAAGAAA GGACTTTTTA ACCTCAGCCT GGCCACTCAG
    ATATCCGATT TTCTTTCTGT GCCCCTGTGG AAGGAGAGTC CTAACCCAGC TCTAACATTC
    TGTGTCAAGA CTCACGATCG CCTGTATTAC ATGGTAGCTC CTTCCCCAGA GGCTATGCGG
    ATATGGATGG ATGTCATTGT AACTGGGGCA GAGGGTTACA CTCAGTTCAT GAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821633.1
    CDS35..4111
    Translation

    Target ORF information:

    RefSeq Version XM_012966179.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966179.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA
    GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG
    CAAGAGATTT CTCGCTCTCG GACAGAAATT GAGGGTGGAG GCACTCTTCC ACGGATGAAA
    ACAGTCTCCC CCGCTTCTTC TCACTTTTCA GTGGCCACTC TGGGCCGTAG TGTTTCCCCA
    AAGAATGCCC ATCTGACTCA GAATGGCACA GGCAGCCTCT CCAGGGGATT GGCAGTAACC
    ATGCAAGACA TTCAAAGCAA GAGACAGCTG GCACTGCAAC AAAAAGGTCA ACAAGTGATA
    GAGGAGCAGC GCAGACGTCT TGCAGAGTTG AAACAGAGAG CTGCAGTTGA GGCACAATCT
    CAGTGGGAAT CTCTCCATGG AGCCCCTAAT CAAGGGTACA CGCCTATAAT TCACCACTCC
    ATTCTACACC ATCAGCGGCC TCTCCATTCT CGTGAAGATG ACCCTACCCA TGGATTTGAC
    ACTCTCAGTC TGGAGAGTTC TGATAGTTTG GACACTAGTG TTTCTACAGG AAACTCTGCA
    TGTTCCCCTG ATAACATGTC AAGTGCTAGT GGGTTAGACA TGCTAAAGAT AGAGGAAATG
    GAAAGAATGC TTCTAGAAGC TCATGCAGAG CGTGCCAGGC TTGTAGAATC CAGGGAGCGT
    GAGGTGGAGG CACGCAGAAA AGCACTAGAA GATGAGAGGC GAAGGAGAGA GGAGCTGGAA
    AGGCGTCTTC AAGATGAGAC AGCTCACAGG CAAAGACTAG TAGAGAAGGA GGTCAAGATG
    AGGGAGAAGC ATTTTTCACA GGCTCGTCCT CTTACACGCT ATCTGCCAAT CCGGAAGGAA
    GACTTTGATC TCCGAGCCCA TGTTGAGTCC TCAGGCCATG CAGTAGATGC CTGCCCTCAC
    ATCATTTTAA GTGAGAAGAT GTGTCGTGGC TTCCTGACAA AAATGGGTGG AAAGATAAAA
    TCATGGAAGA AGCGCTGGTT TGTTTTTGAT CGCCTGAAAC GGACCCTGTC CTATTATGTA
    GATAAACATG AGGCAAAGCT GAAAGGAGTT ATATATTTCC AGGCCATAGA AGAGGTTTAT
    TATGACCACC TCAGGAGTGC AGCCAAGAAA GGACTTTTTA ACCTCAGCCT GGCCACTCAG
    ATATCCGATT TTCTTTCTGT GCCCCTGTGG AAGGAGAGTC CTAACCCAGC TCTAACATTC
    TGTGTCAAGA CTCACGATCG CCTGTATTAC ATGGTAGCTC CTTCCCCAGA GGCTATGCGG
    ATATGGATGG ATGTCATTGT AACTGGGGCA GAGGGTTACA CTCAGTTCAT GAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32418
    Clone ID Related Accession (Same CDS sequence) XM_012966180.2 , XM_012966180.3
    Accession Version XM_012966180.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4071bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pleckstrin homology-like domain family B member 1 isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966180.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTCTGTC TGGGTCAGTC CACCTTCCTG AGATTTAACC ACCCAGCTGA AGCTCACTGG 
    ATGAAAACCA TGATTCCTTC CACTGGGAGA AGCCCTGCGC CAGGCTTGTA CCCATCAGAT
    TCTTCTTCCT CCTTGCTAAA TGGTTCTCGT GATGCTGCAT ACCCGAGAAC TGTACCTTCC
    CATACCTCAC TAGTAAGCTC CATTGAAAAA AATCTTCAGG ACATCATGGA CTCGTTGGTT
    TTAGAAGGGC CTGCACCACT GAGCAGGAAG CTTTCCCAGA CCTCACTTAA CCATATCACC
    CCTATGACCA ATGGAGAGAA CAGACGGTAC TTGCTTTCAT CTCCAACAAG TCCACCTTAC
    TCAAACTCAG GTTATGACAA TACCTCTCCA CTGTCTTCTC CTGGAAGTGG AAGTCTCACT
    AGCCCCTCTC CGGGTCAAGA TGCAGGAACG CCAACAGCAC CCCCTGTGGT GCCACTTCGA
    ACTTCTAGCT TTAGCCATTC CTTGCCACGA CCAGTTCCTA CCTATGGAGG AAGCAACCTT
    GACTTGCGTT CTAATCGCAA CACAGGTGGT GGGTCTCTGC TTAGTCCTTC AAGCCCAGCA
    ACAGGAAGCA GGATGGGGGG ACTAAGTGTG CCAAGCAGTC CACTCCTAGG TAGTAAGTTT
    CATCACCAGA TCACAGACCG CCCAACCAGC CCTTTTAGAC AGGTGGTAGG ACCAACACGG
    AGTCTGCCAC CAGAGAGTCC AAGACTTGGG CGGAGGAACT TTGAAAGCAT GAGGGACCTG
    CCTCCACTTA GCCCTTCTCT GCCCCGAAGA AGTTCATCTA GCTCCATTAA ACCTGCTCCA
    GACAGCCCAT CTCGAGTTCC AGACAGCCCC AGCACTTTAG GGCGACGTCG GGTGGGAAGT
    GCTAGCTCTC CTGGCACAGA AAATAATGCA AGTACAAGGC AGATAAGGGA GCGCAGCCCA
    TCTCCCTCCT CTCTTCAAGA GACCTCTCGT CGACTAACTC CAGCTTATAG CCTGGGATCG
    CTTGGGACCC CTTCACAAAG CCCCAGGGCA CAAAGAAAAG GGCCTGTGCT ACGGGAGCGC
    AAGAACAGCA TCAGTGAAAT TTCTGGCAAC CAAGAGGAGC TAATGGACTA CCACAGAAGG
    CAGAGAGAAG AAAGACTTAG AGAGCAGGAA ATGGAAAGGC TGGAGCGGCA GAGACTGGAA
    ACCATTTTAA ACTTGTGTGC CGAATACAGT AAAACAGATG ATACAGCTCC CCCAGCTCGC
    CTAGATGAAA TAAGGAGCTT CCCCACTTTG GGGGGTACTG AGGAAACCAC TGCATCCCTC
    TTCACTCGTA GACCTTCTTG GGAGAGCACC AGAAGTACCA ACCTGGAGGG AACAGGCAAT
    AGGGTGAGAG CACAGGGAGA ACACAATGAG GAAGAGACTC AGAGAGAAGA GAACAGCAGT
    ACAGAGAGTA CCCAACATGA GCAGGAAGAT GCTGTAAGCC CAACTGGGGC TGCAGAATTA
    CTAGCCCTGG AGGAGCAACG CAGGCACACC CTGGGGCATG TGGATCAGCT AAAGAATAGA
    GTGACTGAAT TGGAGGGTCA GTTGCAGGAA AGTGCAAGGG AGGCAGAGAT GGAACGTGCC
    CTTTTGCAAG GGGAACGGGA GGCTGAGCTG TCCCAGTTGC AGCATGAGCA AAGAATGGTC
    CAGCAGCTGC AGGACAGCCT GACCTCTTTA GAAAACAACT TGCAGAAGGA GAAAGACAAG
    GCACGTGAGA AACTGGAGCA GGAGAGGATG GAGCTGCAGA GTATTCAGCT TTCTCACCAC
    GACCTGCAGA GGCAGCTCCA TAACTGCCCT GAGGCTCTGC GGGAGCAGGT GCAGTGCCAG
    CTGACACGGG AGTCTGAACT CCTGGAGACG AAGATGAAGC TGTTTGAGGA TCTGGAGTTC
    CAGCAGCTAG AAAAGGAAAG CCGCCTGGAA GAAGAAAGAG AGACCACAAG TCAACAACTT
    TTACAGAGCA AGGGGGAATG CCAGCGAAGC ATTGCACGCA GGAAGGAACG CCTGGCTGCT
    TTGGAGATGC AAGCCAATCA GATTCGCCTC CAGGCAGCAC AGGATGCAGA CCATCTTGGC
    CAGGAAAGAA GCGCCATACT TTTACAGCTA CACAAGGAGA AAGAGAAGCT GGTTCAGTTG
    GAGAGAAGAT ACCAGGCACT AACAGGACGA CGGGGATTCT CAACTGGATC AGCTGCTCTC
    CGAGAGGATG CTCTTCAAAT CTATGAGACT AGAGACTGCT TACTCGCTCA AGCTGATGTT
    TCTTGCACAC CTGCTATCCC TTCTGCCTGC TCTCACTCCC CTTCCCGGCA GCAAGAGGAA
    TACCTGTGCC TCTCTGATGT TCTCCGCTTG TACAGCAGCT CGCCTCTTGT TCCTGCTTTT
    CCCCTTCTCT CTGCTGCAGA GCCCACCGCC GCCCTTGAGT ACATCACTTT AGATCAGCTG
    CAGGATATTC TGGAAAGCAT GCCCACAGGT TCTGCTCCAA CTGCTGTGGC AATGGCTGGC
    ACTGATATCA ACTCTGCCTC TTGTGCTCCT TCACCTGACG ACCCTCCTCC TGTACATCTC
    TCGTCTTCAT CTTCTTCCTC TCCCTCCATT TCTCTGGAGG TGAATTCCCC AACAACAGAA
    GGTCACTCTG CCCCCCTAGC ACCTGATTTG GATGCCTATT ACCAAGATTT AGTAACTGCA
    CCTTCTGCCT CTTCTTCTCC ATCTTCTTCC CCTCCTCCAC TCCCTGCTAA ATCCCACTCA
    TCACGTAAGG CTTTGCAGAT TTCTCGCTCT CGGACAGAAA TTGAGGGTGG AGGCACTCTT
    CCACGGATGA AAACAGTCTC CCCCGCTTCT TCTCACTTTT CAGTGGCCAC TCTGGGCCGT
    AGTGTTTCCC CAAAGAATGC CCATCTGACT CAGAATGGCA CAGGCAGCCT CTCCAGGGGA
    TTGGCAGTAA CCATGCAAGA CATTCAAAGC AAGAGACAGC TGGCACTGCA ACAAAAAGAC
    AGTCTGAGCA CAGAAGATTC TCTTCCATCT GAAGTTGAAG GTCAACAAGT GATAGAGGAG
    CAGCGCAGAC GTCTTGCAGA GTTGAAACAG AGAGCTGCAG TTGAGGCACA ATCTCAGTGG
    GAATCTCTCC ATGGAGCCCC TAATCAAGGG TACACGCCTA TAATTCACCA CTCCATTCTA
    CACCATCAGC GGCCTCTCCA TTCTCGTGAA GATGACCCTA CCCATGGATT TGACACTCTC
    AGTCTGGAGA GTTCTGATAG TTTGGACACT AGTGTTTCTA CAGGAAACTC TGCATGTTCC
    CCTGATAACA TGTCAAGTGC TAGTGGGTTA GACATGCTAA AGATAGAGGA AATGGAAAGA
    ATGCTTCTAG AAGCTCATGC AGAGCGTGCC AGGCTTGTAG AATCCAGGGA GCGTGAGGTG
    GAGGCACGCA GAAAAGCACT AGAAGATGAG AGGCGAAGGA GAGAGGAGCT GGAAAGGCGT
    CTTCAAGATG AGACAGCTCA CAGGCAAAGA CTAGTAGAGA AGGAGGTCAA GATGAGGGAG
    AAGCATTTTT CACAGGCTCG TCCTCTTACA CGCTATCTGC CAATCCGGAA GGAAGACTTT
    GATCTCCGAG CCCATGTTGA GTCCTCAGGC CATGCAGTAG ATGCCTGCCC TCACATCATT
    TTAAGTGAGA AGATGTGTCG TGGCTTCCTG ACAAAAATGG GTGGAAAGAT AAAATCATGG
    AAGAAGCGCT GGTTTGTTTT TGATCGCCTG AAACGGACCC TGTCCTATTA TGTAGATAAA
    CATGAGGCAA AGCTGAAAGG AGTTATATAT TTCCAGGCCA TAGAAGAGGT TTATTATGAC
    CACCTCAGGA GTGCAGCCAA GAAAGGACTT TTTAACCTCA GCCTGGCCAC TCAGATATCC
    GATTTTCTTT CTGTGCCCCT GTGGAAGGAG AGTCCTAACC CAGCTCTAAC ATTCTGTGTC
    AAGACTCACG ATCGCCTGTA TTACATGGTA GCTCCTTCCC CAGAGGCTAT GCGGATATGG
    ATGGATGTCA TTGTAACTGG GGCAGAGGGT TACACTCAGT TCATGAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821634.1
    CDS622..4692
    Translation

    Target ORF information:

    RefSeq Version XM_012966180.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966180.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTCTGTC TGGGTCAGTC CACCTTCCTG AGATTTAACC ACCCAGCTGA AGCTCACTGG 
    ATGAAAACCA TGATTCCTTC CACTGGGAGA AGCCCTGCGC CAGGCTTGTA CCCATCAGAT
    TCTTCTTCCT CCTTGCTAAA TGGTTCTCGT GATGCTGCAT ACCCGAGAAC TGTACCTTCC
    CATACCTCAC TAGTAAGCTC CATTGAAAAA AATCTTCAGG ACATCATGGA CTCGTTGGTT
    TTAGAAGGGC CTGCACCACT GAGCAGGAAG CTTTCCCAGA CCTCACTTAA CCATATCACC
    CCTATGACCA ATGGAGAGAA CAGACGGTAC TTGCTTTCAT CTCCAACAAG TCCACCTTAC
    TCAAACTCAG GTTATGACAA TACCTCTCCA CTGTCTTCTC CTGGAAGTGG AAGTCTCACT
    AGCCCCTCTC CGGGTCAAGA TGCAGGAACG CCAACAGCAC CCCCTGTGGT GCCACTTCGA
    ACTTCTAGCT TTAGCCATTC CTTGCCACGA CCAGTTCCTA CCTATGGAGG AAGCAACCTT
    GACTTGCGTT CTAATCGCAA CACAGGTGGT GGGTCTCTGC TTAGTCCTTC AAGCCCAGCA
    ACAGGAAGCA GGATGGGGGG ACTAAGTGTG CCAAGCAGTC CACTCCTAGG TAGTAAGTTT
    CATCACCAGA TCACAGACCG CCCAACCAGC CCTTTTAGAC AGGTGGTAGG ACCAACACGG
    AGTCTGCCAC CAGAGAGTCC AAGACTTGGG CGGAGGAACT TTGAAAGCAT GAGGGACCTG
    CCTCCACTTA GCCCTTCTCT GCCCCGAAGA AGTTCATCTA GCTCCATTAA ACCTGCTCCA
    GACAGCCCAT CTCGAGTTCC AGACAGCCCC AGCACTTTAG GGCGACGTCG GGTGGGAAGT
    GCTAGCTCTC CTGGCACAGA AAATAATGCA AGTACAAGGC AGATAAGGGA GCGCAGCCCA
    TCTCCCTCCT CTCTTCAAGA GACCTCTCGT CGACTAACTC CAGCTTATAG CCTGGGATCG
    CTTGGGACCC CTTCACAAAG CCCCAGGGCA CAAAGAAAAG GGCCTGTGCT ACGGGAGCGC
    AAGAACAGCA TCAGTGAAAT TTCTGGCAAC CAAGAGGAGC TAATGGACTA CCACAGAAGG
    CAGAGAGAAG AAAGACTTAG AGAGCAGGAA ATGGAAAGGC TGGAGCGGCA GAGACTGGAA
    ACCATTTTAA ACTTGTGTGC CGAATACAGT AAAACAGATG ATACAGCTCC CCCAGCTCGC
    CTAGATGAAA TAAGGAGCTT CCCCACTTTG GGGGGTACTG AGGAAACCAC TGCATCCCTC
    TTCACTCGTA GACCTTCTTG GGAGAGCACC AGAAGTACCA ACCTGGAGGG AACAGGCAAT
    AGGGTGAGAG CACAGGGAGA ACACAATGAG GAAGAGACTC AGAGAGAAGA GAACAGCAGT
    ACAGAGAGTA CCCAACATGA GCAGGAAGAT GCTGTAAGCC CAACTGGGGC TGCAGAATTA
    CTAGCCCTGG AGGAGCAACG CAGGCACACC CTGGGGCATG TGGATCAGCT AAAGAATAGA
    GTGACTGAAT TGGAGGGTCA GTTGCAGGAA AGTGCAAGGG AGGCAGAGAT GGAACGTGCC
    CTTTTGCAAG GGGAACGGGA GGCTGAGCTG TCCCAGTTGC AGCATGAGCA AAGAATGGTC
    CAGCAGCTGC AGGACAGCCT GACCTCTTTA GAAAACAACT TGCAGAAGGA GAAAGACAAG
    GCACGTGAGA AACTGGAGCA GGAGAGGATG GAGCTGCAGA GTATTCAGCT TTCTCACCAC
    GACCTGCAGA GGCAGCTCCA TAACTGCCCT GAGGCTCTGC GGGAGCAGGT GCAGTGCCAG
    CTGACACGGG AGTCTGAACT CCTGGAGACG AAGATGAAGC TGTTTGAGGA TCTGGAGTTC
    CAGCAGCTAG AAAAGGAAAG CCGCCTGGAA GAAGAAAGAG AGACCACAAG TCAACAACTT
    TTACAGAGCA AGGGGGAATG CCAGCGAAGC ATTGCACGCA GGAAGGAACG CCTGGCTGCT
    TTGGAGATGC AAGCCAATCA GATTCGCCTC CAGGCAGCAC AGGATGCAGA CCATCTTGGC
    CAGGAAAGAA GCGCCATACT TTTACAGCTA CACAAGGAGA AAGAGAAGCT GGTTCAGTTG
    GAGAGAAGAT ACCAGGCACT AACAGGACGA CGGGGATTCT CAACTGGATC AGCTGCTCTC
    CGAGAGGATG CTCTTCAAAT CTATGAGACT AGAGACTGCT TACTCGCTCA AGCTGATGTT
    TCTTGCACAC CTGCTATCCC TTCTGCCTGC TCTCACTCCC CTTCCCGGCA GCAAGAGGAA
    TACCTGTGCC TCTCTGATGT TCTCCGCTTG TACAGCAGCT CGCCTCTTGT TCCTGCTTTT
    CCCCTTCTCT CTGCTGCAGA GCCCACCGCC GCCCTTGAGT ACATCACTTT AGATCAGCTG
    CAGGATATTC TGGAAAGCAT GCCCACAGGT TCTGCTCCAA CTGCTGTGGC AATGGCTGGC
    ACTGATATCA ACTCTGCCTC TTGTGCTCCT TCACCTGACG ACCCTCCTCC TGTACATCTC
    TCGTCTTCAT CTTCTTCCTC TCCCTCCATT TCTCTGGAGG TGAATTCCCC AACAACAGAA
    GGTCACTCTG CCCCCCTAGC ACCTGATTTG GATGCCTATT ACCAAGATTT AGTAACTGCA
    CCTTCTGCCT CTTCTTCTCC ATCTTCTTCC CCTCCTCCAC TCCCTGCTAA ATCCCACTCA
    TCACGTAAGG CTTTGCAGAT TTCTCGCTCT CGGACAGAAA TTGAGGGTGG AGGCACTCTT
    CCACGGATGA AAACAGTCTC CCCCGCTTCT TCTCACTTTT CAGTGGCCAC TCTGGGCCGT
    AGTGTTTCCC CAAAGAATGC CCATCTGACT CAGAATGGCA CAGGCAGCCT CTCCAGGGGA
    TTGGCAGTAA CCATGCAAGA CATTCAAAGC AAGAGACAGC TGGCACTGCA ACAAAAAGAC
    AGTCTGAGCA CAGAAGATTC TCTTCCATCT GAAGTTGAAG GTCAACAAGT GATAGAGGAG
    CAGCGCAGAC GTCTTGCAGA GTTGAAACAG AGAGCTGCAG TTGAGGCACA ATCTCAGTGG
    GAATCTCTCC ATGGAGCCCC TAATCAAGGG TACACGCCTA TAATTCACCA CTCCATTCTA
    CACCATCAGC GGCCTCTCCA TTCTCGTGAA GATGACCCTA CCCATGGATT TGACACTCTC
    AGTCTGGAGA GTTCTGATAG TTTGGACACT AGTGTTTCTA CAGGAAACTC TGCATGTTCC
    CCTGATAACA TGTCAAGTGC TAGTGGGTTA GACATGCTAA AGATAGAGGA AATGGAAAGA
    ATGCTTCTAG AAGCTCATGC AGAGCGTGCC AGGCTTGTAG AATCCAGGGA GCGTGAGGTG
    GAGGCACGCA GAAAAGCACT AGAAGATGAG AGGCGAAGGA GAGAGGAGCT GGAAAGGCGT
    CTTCAAGATG AGACAGCTCA CAGGCAAAGA CTAGTAGAGA AGGAGGTCAA GATGAGGGAG
    AAGCATTTTT CACAGGCTCG TCCTCTTACA CGCTATCTGC CAATCCGGAA GGAAGACTTT
    GATCTCCGAG CCCATGTTGA GTCCTCAGGC CATGCAGTAG ATGCCTGCCC TCACATCATT
    TTAAGTGAGA AGATGTGTCG TGGCTTCCTG ACAAAAATGG GTGGAAAGAT AAAATCATGG
    AAGAAGCGCT GGTTTGTTTT TGATCGCCTG AAACGGACCC TGTCCTATTA TGTAGATAAA
    CATGAGGCAA AGCTGAAAGG AGTTATATAT TTCCAGGCCA TAGAAGAGGT TTATTATGAC
    CACCTCAGGA GTGCAGCCAA GAAAGGACTT TTTAACCTCA GCCTGGCCAC TCAGATATCC
    GATTTTCTTT CTGTGCCCCT GTGGAAGGAG AGTCCTAACC CAGCTCTAAC ATTCTGTGTC
    AAGACTCACG ATCGCCTGTA TTACATGGTA GCTCCTTCCC CAGAGGCTAT GCGGATATGG
    ATGGATGTCA TTGTAACTGG GGCAGAGGGT TACACTCAGT TCATGAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32419
    Clone ID Related Accession (Same CDS sequence) XM_012966182.3 , XM_012966182.2
    Accession Version XM_012966182.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4008bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pleckstrin homology-like domain family B member 1 isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966182.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGATTTC TCGCTCTCGG ACAGAAATTG AGGGTGGAGG CACTCTTCCA
    CGGATGAAAA CAGTCTCCCC CGCTTCTTCT CACTTTTCAG TGGCCACTCT GGGCCGTAGT
    GTTTCCCCAA AGAATGCCCA TCTGACTCAG AATGGCACAG GCAGCCTCTC CAGGGGATTG
    GCAGTAACCA TGCAAGACAT TCAAAGCAAG AGACAGCTGG CACTGCAACA AAAAGACAGT
    CTGAGCACAG AAGATTCTCT TCCATCTGAA GTTGAAGGTC AACAAGTGAT AGAGGAGCAG
    CGCAGACGTC TTGCAGAGTT GAAACAGAGA GCTGCAGTTG AGGCACAATC TCAGTGGGAA
    TCTCTCCATG GAGCCCCTAA TCAAGGGTAC ACGCCTATAA TTCACCACTC CATTCTACAC
    CATCAGCGGC CTCTCCATTC TCGTGAAGAT GACCCTACCC ATGGATTTGA CACTCTCAGT
    CTGGAGAGTT CTGATAGTTT GGACACTAGT GTTTCTACAG GAAACTCTGC ATGTTCCCCT
    GATAACATGT CAAGTGCTAG TGGGTTAGAC ATGCTAAAGA TAGAGGAAAT GGAAAGAATG
    CTTCTAGAAG CTCATGCAGA GCGTGCCAGG CTTGTAGAAT CCAGGGAGCG TGAGGTGGAG
    GCACGCAGAA AAGCACTAGA AGATGAGAGG CGAAGGAGAG AGGAGCTGGA AAGGCGTCTT
    CAAGATGAGA CAGCTCACAG GCAAAGACTA GTAGAGAAGG AGGTCAAGAT GAGGGAGAAG
    CATTTTTCAC AGGCTCGTCC TCTTACACGC TATCTGCCAA TCCGGAAGGA AGACTTTGAT
    CTCCGAGCCC ATGTTGAGTC CTCAGGCCAT GCAGTAGATG CCTGCCCTCA CATCATTTTA
    AGTGAGAAGA TGTGTCGTGG CTTCCTGACA AAAATGGGTG GAAAGATAAA ATCATGGAAG
    AAGCGCTGGT TTGTTTTTGA TCGCCTGAAA CGGACCCTGT CCTATTATGT AGATAAACAT
    GAGGCAAAGC TGAAAGGAGT TATATATTTC CAGGCCATAG AAGAGGTTTA TTATGACCAC
    CTCAGGAGTG CAGCCAAGAA AGGACTTTTT AACCTCAGCC TGGCCACTCA GATATCCGAT
    TTTCTTTCTG TGCCCCTGTG GAAGGAGAGT CCTAACCCAG CTCTAACATT CTGTGTCAAG
    ACTCACGATC GCCTGTATTA CATGGTAGCT CCTTCCCCAG AGGCTATGCG GATATGGATG
    GATGTCATTG TAACTGGGGC AGAGGGTTAC ACTCAGTTCA TGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821636.1
    CDS35..4042
    Translation

    Target ORF information:

    RefSeq Version XM_012966182.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966182.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGATTTC TCGCTCTCGG ACAGAAATTG AGGGTGGAGG CACTCTTCCA
    CGGATGAAAA CAGTCTCCCC CGCTTCTTCT CACTTTTCAG TGGCCACTCT GGGCCGTAGT
    GTTTCCCCAA AGAATGCCCA TCTGACTCAG AATGGCACAG GCAGCCTCTC CAGGGGATTG
    GCAGTAACCA TGCAAGACAT TCAAAGCAAG AGACAGCTGG CACTGCAACA AAAAGACAGT
    CTGAGCACAG AAGATTCTCT TCCATCTGAA GTTGAAGGTC AACAAGTGAT AGAGGAGCAG
    CGCAGACGTC TTGCAGAGTT GAAACAGAGA GCTGCAGTTG AGGCACAATC TCAGTGGGAA
    TCTCTCCATG GAGCCCCTAA TCAAGGGTAC ACGCCTATAA TTCACCACTC CATTCTACAC
    CATCAGCGGC CTCTCCATTC TCGTGAAGAT GACCCTACCC ATGGATTTGA CACTCTCAGT
    CTGGAGAGTT CTGATAGTTT GGACACTAGT GTTTCTACAG GAAACTCTGC ATGTTCCCCT
    GATAACATGT CAAGTGCTAG TGGGTTAGAC ATGCTAAAGA TAGAGGAAAT GGAAAGAATG
    CTTCTAGAAG CTCATGCAGA GCGTGCCAGG CTTGTAGAAT CCAGGGAGCG TGAGGTGGAG
    GCACGCAGAA AAGCACTAGA AGATGAGAGG CGAAGGAGAG AGGAGCTGGA AAGGCGTCTT
    CAAGATGAGA CAGCTCACAG GCAAAGACTA GTAGAGAAGG AGGTCAAGAT GAGGGAGAAG
    CATTTTTCAC AGGCTCGTCC TCTTACACGC TATCTGCCAA TCCGGAAGGA AGACTTTGAT
    CTCCGAGCCC ATGTTGAGTC CTCAGGCCAT GCAGTAGATG CCTGCCCTCA CATCATTTTA
    AGTGAGAAGA TGTGTCGTGG CTTCCTGACA AAAATGGGTG GAAAGATAAA ATCATGGAAG
    AAGCGCTGGT TTGTTTTTGA TCGCCTGAAA CGGACCCTGT CCTATTATGT AGATAAACAT
    GAGGCAAAGC TGAAAGGAGT TATATATTTC CAGGCCATAG AAGAGGTTTA TTATGACCAC
    CTCAGGAGTG CAGCCAAGAA AGGACTTTTT AACCTCAGCC TGGCCACTCA GATATCCGAT
    TTTCTTTCTG TGCCCCTGTG GAAGGAGAGT CCTAACCCAG CTCTAACATT CTGTGTCAAG
    ACTCACGATC GCCTGTATTA CATGGTAGCT CCTTCCCCAG AGGCTATGCG GATATGGATG
    GATGTCATTG TAACTGGGGC AGAGGGTTAC ACTCAGTTCA TGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32420
    Clone ID Related Accession (Same CDS sequence) XM_012966183.2 , XM_012966183.3
    Accession Version XM_012966183.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3966bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pleckstrin homology-like domain family B member 1 isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966183.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGATTTC TCGCTCTCGG ACAGAAATTG AGGGTGGAGG CACTCTTCCA
    CGGATGAAAA CAGTCTCCCC CGCTTCTTCT CACTTTTCAG TGGCCACTCT GGGCCGTAGT
    GTTTCCCCAA AGAATGCCCA TCTGACTCAG AATGGCACAG GCAGCCTCTC CAGGGGATTG
    GCAGTAACCA TGCAAGACAT TCAAAGCAAG AGACAGCTGG CACTGCAACA AAAAGGTCAA
    CAAGTGATAG AGGAGCAGCG CAGACGTCTT GCAGAGTTGA AACAGAGAGC TGCAGTTGAG
    GCACAATCTC AGTGGGAATC TCTCCATGGA GCCCCTAATC AAGGGTACAC GCCTATAATT
    CACCACTCCA TTCTACACCA TCAGCGGCCT CTCCATTCTC GTGAAGATGA CCCTACCCAT
    GGATTTGACA CTCTCAGTCT GGAGAGTTCT GATAGTTTGG ACACTAGTGT TTCTACAGGA
    AACTCTGCAT GTTCCCCTGA TAACATGTCA AGTGCTAGTG GGTTAGACAT GCTAAAGATA
    GAGGAAATGG AAAGAATGCT TCTAGAAGCT CATGCAGAGC GTGCCAGGCT TGTAGAATCC
    AGGGAGCGTG AGGTGGAGGC ACGCAGAAAA GCACTAGAAG ATGAGAGGCG AAGGAGAGAG
    GAGCTGGAAA GGCGTCTTCA AGATGAGACA GCTCACAGGC AAAGACTAGT AGAGAAGGAG
    GTCAAGATGA GGGAGAAGCA TTTTTCACAG GCTCGTCCTC TTACACGCTA TCTGCCAATC
    CGGAAGGAAG ACTTTGATCT CCGAGCCCAT GTTGAGTCCT CAGGCCATGC AGTAGATGCC
    TGCCCTCACA TCATTTTAAG TGAGAAGATG TGTCGTGGCT TCCTGACAAA AATGGGTGGA
    AAGATAAAAT CATGGAAGAA GCGCTGGTTT GTTTTTGATC GCCTGAAACG GACCCTGTCC
    TATTATGTAG ATAAACATGA GGCAAAGCTG AAAGGAGTTA TATATTTCCA GGCCATAGAA
    GAGGTTTATT ATGACCACCT CAGGAGTGCA GCCAAGAAAG GACTTTTTAA CCTCAGCCTG
    GCCACTCAGA TATCCGATTT TCTTTCTGTG CCCCTGTGGA AGGAGAGTCC TAACCCAGCT
    CTAACATTCT GTGTCAAGAC TCACGATCGC CTGTATTACA TGGTAGCTCC TTCCCCAGAG
    GCTATGCGGA TATGGATGGA TGTCATTGTA ACTGGGGCAG AGGGTTACAC TCAGTTCATG
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821637.1
    CDS35..4000
    Translation

    Target ORF information:

    RefSeq Version XM_012966183.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966183.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGATTTC TCGCTCTCGG ACAGAAATTG AGGGTGGAGG CACTCTTCCA
    CGGATGAAAA CAGTCTCCCC CGCTTCTTCT CACTTTTCAG TGGCCACTCT GGGCCGTAGT
    GTTTCCCCAA AGAATGCCCA TCTGACTCAG AATGGCACAG GCAGCCTCTC CAGGGGATTG
    GCAGTAACCA TGCAAGACAT TCAAAGCAAG AGACAGCTGG CACTGCAACA AAAAGGTCAA
    CAAGTGATAG AGGAGCAGCG CAGACGTCTT GCAGAGTTGA AACAGAGAGC TGCAGTTGAG
    GCACAATCTC AGTGGGAATC TCTCCATGGA GCCCCTAATC AAGGGTACAC GCCTATAATT
    CACCACTCCA TTCTACACCA TCAGCGGCCT CTCCATTCTC GTGAAGATGA CCCTACCCAT
    GGATTTGACA CTCTCAGTCT GGAGAGTTCT GATAGTTTGG ACACTAGTGT TTCTACAGGA
    AACTCTGCAT GTTCCCCTGA TAACATGTCA AGTGCTAGTG GGTTAGACAT GCTAAAGATA
    GAGGAAATGG AAAGAATGCT TCTAGAAGCT CATGCAGAGC GTGCCAGGCT TGTAGAATCC
    AGGGAGCGTG AGGTGGAGGC ACGCAGAAAA GCACTAGAAG ATGAGAGGCG AAGGAGAGAG
    GAGCTGGAAA GGCGTCTTCA AGATGAGACA GCTCACAGGC AAAGACTAGT AGAGAAGGAG
    GTCAAGATGA GGGAGAAGCA TTTTTCACAG GCTCGTCCTC TTACACGCTA TCTGCCAATC
    CGGAAGGAAG ACTTTGATCT CCGAGCCCAT GTTGAGTCCT CAGGCCATGC AGTAGATGCC
    TGCCCTCACA TCATTTTAAG TGAGAAGATG TGTCGTGGCT TCCTGACAAA AATGGGTGGA
    AAGATAAAAT CATGGAAGAA GCGCTGGTTT GTTTTTGATC GCCTGAAACG GACCCTGTCC
    TATTATGTAG ATAAACATGA GGCAAAGCTG AAAGGAGTTA TATATTTCCA GGCCATAGAA
    GAGGTTTATT ATGACCACCT CAGGAGTGCA GCCAAGAAAG GACTTTTTAA CCTCAGCCTG
    GCCACTCAGA TATCCGATTT TCTTTCTGTG CCCCTGTGGA AGGAGAGTCC TAACCCAGCT
    CTAACATTCT GTGTCAAGAC TCACGATCGC CTGTATTACA TGGTAGCTCC TTCCCCAGAG
    GCTATGCGGA TATGGATGGA TGTCATTGTA ACTGGGGCAG AGGGTTACAC TCAGTTCATG
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32421
    Clone ID Related Accession (Same CDS sequence) XM_012966184.2 , XM_012966184.3
    Accession Version XM_012966184.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3837bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pleckstrin homology-like domain family B member 1 isoform X23
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966184.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG AGTCTGAACT CCTGGAGACG AAGATGAAGC TGTTTGAGGA TCTGGAGTTC
    CAGCAGCTAG AAAAGGAAAG CCGCCTGGAA GAAGAAAGAG AGACCACAAG TCAACAACTT
    TTACAGAGCA AGGGGGAATG CCAGCGAAGC ATTGCACGCA GGAAGGAACG CCTGGCTGCT
    TTGGAGATGC AAGCCAATCA GATTCGCCTC CAGGCAGCAC AGGATGCAGA CCATCTTGGC
    CAGGAAAGAA GCGCCATACT TTTACAGCTA CACAAGGAGA AAGAGAAGCT GGTTCAGTTG
    GAGAGAAGAT ACCAGGCACT AACAGGACGA CGGGGATTCT CAACTGGATC AGCTGCTCTC
    CGAGAGATTT CTCGCTCTCG GACAGAAATT GAGGGTGGAG GCACTCTTCC ACGGATGAAA
    ACAGTCTCCC CCGCTTCTTC TCACTTTTCA GTGGCCACTC TGGGCCGTAG TGTTTCCCCA
    AAGAATGCCC ATCTGACTCA GAATGGCACA GGCAGCCTCT CCAGGGGATT GGCAGTAACC
    ATGCAAGACA TTCAAAGCAA GAGACAGCTG GCACTGCAAC AAAAAGGTCA ACAAGTGATA
    GAGGAGCAGC GCAGACGTCT TGCAGAGTTG AAACAGAGAG CTGCAGTTGA GGCACAATCT
    CAGTGGGAAT CTCTCCATGG AGCCCCTAAT CAAGGGTACA CGCCTATAAT TCACCACTCC
    ATTCTACACC ATCAGCGGCC TCTCCATTCT CGTGAAGATG ACCCTACCCA TGGATTTGAC
    ACTCTCAGTC TGGAGAGTTC TGATAGTTTG GACACTAGTG TTTCTACAGG AAACTCTGCA
    TGTTCCCCTG ATAACATGTC AAGTGCTAGT GGGTTAGACA TGCTAAAGAT AGAGGAAATG
    GAAAGAATGC TTCTAGAAGC TCATGCAGAG CGTGCCAGGC TTGTAGAATC CAGGGAGCGT
    GAGGTGGAGG CACGCAGAAA AGCACTAGAA GATGAGAGGC GAAGGAGAGA GGAGCTGGAA
    AGGCGTCTTC AAGATGAGAC AGCTCACAGG CAAAGACTAG TAGAGAAGGA GGTCAAGATG
    AGGGAGAAGC ATTTTTCACA GGCTCGTCCT CTTACACGCT ATCTGCCAAT CCGGAAGGAA
    GACTTTGATC TCCGAGCCCA TGTTGAGTCC TCAGGCCATG CAGTAGATGC CTGCCCTCAC
    ATCATTTTAA GTGAGAAGAT GTGTCGTGGC TTCCTGACAA AAATGGGTGG AAAGATAAAA
    TCATGGAAGA AGCGCTGGTT TGTTTTTGAT CGCCTGAAAC GGACCCTGTC CTATTATGTA
    GATAAACATG AGGCAAAGCT GAAAGGAGTT ATATATTTCC AGGCCATAGA AGAGGTTTAT
    TATGACCACC TCAGGAGTGC AGCCAAGAAA GGACTTTTTA ACCTCAGCCT GGCCACTCAG
    ATATCCGATT TTCTTTCTGT GCCCCTGTGG AAGGAGAGTC CTAACCCAGC TCTAACATTC
    TGTGTCAAGA CTCACGATCG CCTGTATTAC ATGGTAGCTC CTTCCCCAGA GGCTATGCGG
    ATATGGATGG ATGTCATTGT AACTGGGGCA GAGGGTTACA CTCAGTTCAT GAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821638.1
    CDS35..3871
    Translation

    Target ORF information:

    RefSeq Version XM_012966184.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966184.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG AGTCTGAACT CCTGGAGACG AAGATGAAGC TGTTTGAGGA TCTGGAGTTC
    CAGCAGCTAG AAAAGGAAAG CCGCCTGGAA GAAGAAAGAG AGACCACAAG TCAACAACTT
    TTACAGAGCA AGGGGGAATG CCAGCGAAGC ATTGCACGCA GGAAGGAACG CCTGGCTGCT
    TTGGAGATGC AAGCCAATCA GATTCGCCTC CAGGCAGCAC AGGATGCAGA CCATCTTGGC
    CAGGAAAGAA GCGCCATACT TTTACAGCTA CACAAGGAGA AAGAGAAGCT GGTTCAGTTG
    GAGAGAAGAT ACCAGGCACT AACAGGACGA CGGGGATTCT CAACTGGATC AGCTGCTCTC
    CGAGAGATTT CTCGCTCTCG GACAGAAATT GAGGGTGGAG GCACTCTTCC ACGGATGAAA
    ACAGTCTCCC CCGCTTCTTC TCACTTTTCA GTGGCCACTC TGGGCCGTAG TGTTTCCCCA
    AAGAATGCCC ATCTGACTCA GAATGGCACA GGCAGCCTCT CCAGGGGATT GGCAGTAACC
    ATGCAAGACA TTCAAAGCAA GAGACAGCTG GCACTGCAAC AAAAAGGTCA ACAAGTGATA
    GAGGAGCAGC GCAGACGTCT TGCAGAGTTG AAACAGAGAG CTGCAGTTGA GGCACAATCT
    CAGTGGGAAT CTCTCCATGG AGCCCCTAAT CAAGGGTACA CGCCTATAAT TCACCACTCC
    ATTCTACACC ATCAGCGGCC TCTCCATTCT CGTGAAGATG ACCCTACCCA TGGATTTGAC
    ACTCTCAGTC TGGAGAGTTC TGATAGTTTG GACACTAGTG TTTCTACAGG AAACTCTGCA
    TGTTCCCCTG ATAACATGTC AAGTGCTAGT GGGTTAGACA TGCTAAAGAT AGAGGAAATG
    GAAAGAATGC TTCTAGAAGC TCATGCAGAG CGTGCCAGGC TTGTAGAATC CAGGGAGCGT
    GAGGTGGAGG CACGCAGAAA AGCACTAGAA GATGAGAGGC GAAGGAGAGA GGAGCTGGAA
    AGGCGTCTTC AAGATGAGAC AGCTCACAGG CAAAGACTAG TAGAGAAGGA GGTCAAGATG
    AGGGAGAAGC ATTTTTCACA GGCTCGTCCT CTTACACGCT ATCTGCCAAT CCGGAAGGAA
    GACTTTGATC TCCGAGCCCA TGTTGAGTCC TCAGGCCATG CAGTAGATGC CTGCCCTCAC
    ATCATTTTAA GTGAGAAGAT GTGTCGTGGC TTCCTGACAA AAATGGGTGG AAAGATAAAA
    TCATGGAAGA AGCGCTGGTT TGTTTTTGAT CGCCTGAAAC GGACCCTGTC CTATTATGTA
    GATAAACATG AGGCAAAGCT GAAAGGAGTT ATATATTTCC AGGCCATAGA AGAGGTTTAT
    TATGACCACC TCAGGAGTGC AGCCAAGAAA GGACTTTTTA ACCTCAGCCT GGCCACTCAG
    ATATCCGATT TTCTTTCTGT GCCCCTGTGG AAGGAGAGTC CTAACCCAGC TCTAACATTC
    TGTGTCAAGA CTCACGATCG CCTGTATTAC ATGGTAGCTC CTTCCCCAGA GGCTATGCGG
    ATATGGATGG ATGTCATTGT AACTGGGGCA GAGGGTTACA CTCAGTTCAT GAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32422
    Clone ID Related Accession (Same CDS sequence) XM_012966185.2 , XM_012966185.3
    Accession Version XM_012966185.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3768bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pleckstrin homology-like domain family B member 1 isoform X24
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966185.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG AGTCTGAACT CCTGGAGACG AAGATGAAGC TGTTTGAGGA TCTGGAGTTC
    CAGCAGCTAG AAAAGGAAAG CCGCCTGGAA GAAGAAAGAG AGACCACAAG TCAACAACTT
    TTACAGAGCA AGGGGGAATG CCAGCGAAGC ATTGCACGCA GGAAGGAACG CCTGGCTGCT
    TTGGAGATGC AAGCCAATCA GATTCGCCTC CAGGCAGCAC AGGATGCAGA CCATCTTGGC
    CAGGAAAGAA GCGCCATACT TTTACAGCTA CACAAGGAGA AAGAGAAGCT GGTTCAGTTG
    GAGAGAAGAT ACCAGGCACT AACAGGACGA CGGGGATTCT CAACTGGATC AGCTGCTCTC
    CGAGAGATTT CTCGCTCTCG GACAGAAATT GAGGGTGGAG GCACTCTTCC ACGGATGAAA
    ACAGTCTCCC CCGCTTCTTC TCACTTTTCA GTGGCCACTC TGGGCCGTAG TGTTTCCCCA
    AAGAATGCCC ATCTGACTCA GAATGGCACA GGCAGCCTCT CCAGGGGATT GGCAGTAACC
    ATGCAAGACA TTCAAAGCAA GAGACAGCTG GCACTGCAAC AAAAAGGTCA ACAAGTGATA
    GAGGAGCAGC GCAGACGTCT TGCAGAGTTG AAACAGAGAG CTGCAGTTGA GGCACAATCT
    CAGTGGGAAT CTCTCCATGG AGCCCCTAAT CAAGGGTACA CGCCTATAAT TCACCACTCC
    ATTCTACACC ATCAGCGGCC TCTCCATTCT CGTGAAGATG ACCCTACCCA TGGATTTGAC
    ACTCTCAGTC TGGAGAGTTC TGATAGTTTG GACACTAGTG TTTCTACAGG AAACTCTGCA
    TGTTCCCCTG ATAACATGTC AAGTGCTAGT GGGTTAGACA TGCTAAAGAT AGAGGAAATG
    GAAAGAATGC TTCTAGAAGC TCATGCAGAG CGTGCCAGGC TTGTAGAATC CAGGGAGCGT
    GAGGTGGAGG CACGCAGAAA AGCACTAGAA GATGAGAGGC GAAGGAGAGA GGAGCTGGAA
    AGGCGTCTTC AAGATGAGAC AGCTCACAGG CAAAGACTAG TAGAGAAGGA GGTCAAGATG
    AGGGAGAAGC ATTTTTCACA GGCTCGTCCT CTTACACGCT ATCTGCCAAT CCGGAAGGAA
    GACTTTGATC TCCGAGCCCA TGTTGAGTCC TCAGGCCATG CAGTAGATGC CTGCCCTCAC
    ATCATTTTAA GTGAGAAGAT GTGTCGTGGC TTCCTGACAA AAATGGGTGG AAAGATAAAA
    TCATGGAAGA AGCGCTGGTT TGTTTTTGAT CGCCTGAAAC GGACCCTGTC CTATTATGTA
    GATAAACATG AGGCAAAGCT GAAAGGAGTT ATATATTTCC AGGCCATAGA AGAGGTTTAT
    TATGACCACC TCAGGAGTGC AGCCAAGAGT CCTAACCCAG CTCTAACATT CTGTGTCAAG
    ACTCACGATC GCCTGTATTA CATGGTAGCT CCTTCCCCAG AGGCTATGCG GATATGGATG
    GATGTCATTG TAACTGGGGC AGAGGGTTAC ACTCAGTTCA TGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821639.1
    CDS35..3802
    Translation

    Target ORF information:

    RefSeq Version XM_012966185.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966185.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG AGTCTGAACT CCTGGAGACG AAGATGAAGC TGTTTGAGGA TCTGGAGTTC
    CAGCAGCTAG AAAAGGAAAG CCGCCTGGAA GAAGAAAGAG AGACCACAAG TCAACAACTT
    TTACAGAGCA AGGGGGAATG CCAGCGAAGC ATTGCACGCA GGAAGGAACG CCTGGCTGCT
    TTGGAGATGC AAGCCAATCA GATTCGCCTC CAGGCAGCAC AGGATGCAGA CCATCTTGGC
    CAGGAAAGAA GCGCCATACT TTTACAGCTA CACAAGGAGA AAGAGAAGCT GGTTCAGTTG
    GAGAGAAGAT ACCAGGCACT AACAGGACGA CGGGGATTCT CAACTGGATC AGCTGCTCTC
    CGAGAGATTT CTCGCTCTCG GACAGAAATT GAGGGTGGAG GCACTCTTCC ACGGATGAAA
    ACAGTCTCCC CCGCTTCTTC TCACTTTTCA GTGGCCACTC TGGGCCGTAG TGTTTCCCCA
    AAGAATGCCC ATCTGACTCA GAATGGCACA GGCAGCCTCT CCAGGGGATT GGCAGTAACC
    ATGCAAGACA TTCAAAGCAA GAGACAGCTG GCACTGCAAC AAAAAGGTCA ACAAGTGATA
    GAGGAGCAGC GCAGACGTCT TGCAGAGTTG AAACAGAGAG CTGCAGTTGA GGCACAATCT
    CAGTGGGAAT CTCTCCATGG AGCCCCTAAT CAAGGGTACA CGCCTATAAT TCACCACTCC
    ATTCTACACC ATCAGCGGCC TCTCCATTCT CGTGAAGATG ACCCTACCCA TGGATTTGAC
    ACTCTCAGTC TGGAGAGTTC TGATAGTTTG GACACTAGTG TTTCTACAGG AAACTCTGCA
    TGTTCCCCTG ATAACATGTC AAGTGCTAGT GGGTTAGACA TGCTAAAGAT AGAGGAAATG
    GAAAGAATGC TTCTAGAAGC TCATGCAGAG CGTGCCAGGC TTGTAGAATC CAGGGAGCGT
    GAGGTGGAGG CACGCAGAAA AGCACTAGAA GATGAGAGGC GAAGGAGAGA GGAGCTGGAA
    AGGCGTCTTC AAGATGAGAC AGCTCACAGG CAAAGACTAG TAGAGAAGGA GGTCAAGATG
    AGGGAGAAGC ATTTTTCACA GGCTCGTCCT CTTACACGCT ATCTGCCAAT CCGGAAGGAA
    GACTTTGATC TCCGAGCCCA TGTTGAGTCC TCAGGCCATG CAGTAGATGC CTGCCCTCAC
    ATCATTTTAA GTGAGAAGAT GTGTCGTGGC TTCCTGACAA AAATGGGTGG AAAGATAAAA
    TCATGGAAGA AGCGCTGGTT TGTTTTTGAT CGCCTGAAAC GGACCCTGTC CTATTATGTA
    GATAAACATG AGGCAAAGCT GAAAGGAGTT ATATATTTCC AGGCCATAGA AGAGGTTTAT
    TATGACCACC TCAGGAGTGC AGCCAAGAGT CCTAACCCAG CTCTAACATT CTGTGTCAAG
    ACTCACGATC GCCTGTATTA CATGGTAGCT CCTTCCCCAG AGGCTATGCG GATATGGATG
    GATGTCATTG TAACTGGGGC AGAGGGTTAC ACTCAGTTCA TGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32424
    Clone ID Related Accession (Same CDS sequence) XM_012966188.3 , XM_012966188.2
    Accession Version XM_012966188.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4389bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pleckstrin homology-like domain family B member 1 isoform X25
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966188.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG AGTCTGAACT CCTGGAGACG AAGATGAAGC TGTTTGAGGA TCTGGAGTTC
    CAGCAGCTAG AAAAGGAAAG CCGCCTGGAA GAAGAAAGAG AGACCACAAG TCAACAACTT
    TTACAGAGCA AGGGGGAATG CCAGCGAAGC ATTGCACGCA GGAAGGAACG CCTGGCTGCT
    TTGGAGATGC AAGCCAATCA GATTCGCCTC CAGGCAGCAC AGGATGCAGA CCATCTTGGC
    CAGGAAAGAA GCGCCATACT TTTACAGCTA CACAAGGAGA AAGAGAAGCT GGTTCAGTTG
    GAGAGAAGAT ACCAGGCACT AACAGGACGA CGGGGATTCT CAACTGGATC AGCTGCTCTC
    CGAGAGGATG CTCTTCAAAT CTATGAGACT AGAGACTGCT TACTCGCTCA AGCTGATGTT
    TCTTGCACAC CTGCTATCCC TTCTGCCTGC TCTCACTCCC CTTCCCGGCA GCAAGAGGAA
    TACCTGTGCC TCTCTGATGT TCTCCGCTTG TACAGCAGCT CGCCTCTTGT TCCTGCTTTT
    CCCCTTCTCT CTGCTGCAGA GCCCACCGCC GCCCTTGAGT ACATCACTTT AGATCAGCTG
    CAGGATATTC TGGAAAGCAT GCCCACAGGT TCTGCTCCAA CTGCTGTGGC AATGGCTGGC
    ACTGATATCA ACTCTGCCTC TTGTGCTCCT TCACCTGACG ACCCTCCTCC TGTACATCTC
    TCGTCTTCAT CTTCTTCCTC TCCCTCCATT TCTCTGGAGG TGAATTCCCC AACAACAGAA
    GGTCACTCTG CCCCCCTAGC ACCTGATTTG GATGCCTATT ACCAAGATTT AGTAACTGCA
    CCTTCTGCCT CTTCTTCTCC ATCTTCTTCC CCTCCTCCAC TCCCTGCTAA ATCCCACTCA
    TCACGTAAGG CTTTGCAGAT TTCTCGCTCT CGGACAGAAA TTGAGGGTGG AGGCACTCTT
    CCACGGATGA AAACAGTCTC CCCCGCTTCT TCTCACTTTT CAGTGGCCAC TCTGGGCCGT
    AGTGTTTCCC CAAAGAATGC CCATCTGACT CAGAATGGCA CAGGCAGCCT CTCCAGGGGA
    TTGGCAGTAA CCATGCAAGA CATTCAAAGC AAGAGACAGC TGGCACTGCA ACAAAAAGGT
    CAACAAGTGA TAGAGGAGCA GCGCAGACGT CTTGCAGAGT TGAAACAGAG AGCTGCAGTT
    GAGGCACAAT CTCAGTGGGA ATCTCTCCAT GGAGCCCCTA ATCAAGGGTA CACGCCTATA
    ATTCACCACT CCATTCTACA CCATCAGCGG CCTCTCCATT CTCGTGAAGA TGACCCTACC
    CATGGATTTG ACACTCTCAG TCTGGAGAGT TCTGATAGTT TGGACACTAG TGTTTCTACA
    GGAAACTCTG CATGTTCCCC TGATAACATG TCAAGTGCTA GTGGGTTAGA CATGCTAAAG
    ATAGAGGAAA TGGAAAGAAT GCTTCTAGAA GCTCATGCAG AGCGTGCCAG GCTTGTAGAA
    TCCAGGGAGC GTGAGGTGGA GGCACGCAGA AAAGCACTAG AAGATGAGAG GCGAAGGAGA
    GAGGAGCTGG AAAGGCGTCT TCAAGATGAG ACAGCTCACA GGCAAAGACT AGTAGAGAAG
    GAGGTCAAGA TGAGGGAGAA GCATTTTTCA CAGGCTCGTC CTCTTACACG CTATCTGCCA
    ATCCGGAAGG AAGACTTTGA TCTCCGAGCC CATGTTGAGT CCTCAGGCCA TGCAGTAGAT
    GCCTGCCCTC ACATCATTTT AAGTGAGAAG ATGTGTCGTG GCTTCCTGAC AAAAATGGGT
    GGAAAGATAA AATCATGGAA GAAGCGCTGG TTTGTTTTTG ATCGCCTGAA ACGGACCCTG
    TCCTATTATG TAGATAAACA TGAGGCAAAG CTGAAAGGAG TTATATATTT CCAGGCCATA
    GAAGAGGTTT ATTATGACCA CCTCAGGAGT GCAGCCAAGA AAGGACTTTT TAACCTCAGC
    CTGGCCACTC AGATATCCGA TTTTCTTTCT GTGCCCCTGT GGAAGGAGAG TCCTAACCCA
    GCTCTAACAT TCTGTGTCAA GACTCACGAT CGCCTGTATT ACATGGTAGC TCCTTCCCCA
    GAGGCTATGC GGATATGGAT GGATGTCATT GTAACTGGGG CAGAGGGTTA CACTCAGTTC
    ATGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821642.1
    CDS35..4423
    Translation

    Target ORF information:

    RefSeq Version XM_012966188.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966188.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG AGTCTGAACT CCTGGAGACG AAGATGAAGC TGTTTGAGGA TCTGGAGTTC
    CAGCAGCTAG AAAAGGAAAG CCGCCTGGAA GAAGAAAGAG AGACCACAAG TCAACAACTT
    TTACAGAGCA AGGGGGAATG CCAGCGAAGC ATTGCACGCA GGAAGGAACG CCTGGCTGCT
    TTGGAGATGC AAGCCAATCA GATTCGCCTC CAGGCAGCAC AGGATGCAGA CCATCTTGGC
    CAGGAAAGAA GCGCCATACT TTTACAGCTA CACAAGGAGA AAGAGAAGCT GGTTCAGTTG
    GAGAGAAGAT ACCAGGCACT AACAGGACGA CGGGGATTCT CAACTGGATC AGCTGCTCTC
    CGAGAGGATG CTCTTCAAAT CTATGAGACT AGAGACTGCT TACTCGCTCA AGCTGATGTT
    TCTTGCACAC CTGCTATCCC TTCTGCCTGC TCTCACTCCC CTTCCCGGCA GCAAGAGGAA
    TACCTGTGCC TCTCTGATGT TCTCCGCTTG TACAGCAGCT CGCCTCTTGT TCCTGCTTTT
    CCCCTTCTCT CTGCTGCAGA GCCCACCGCC GCCCTTGAGT ACATCACTTT AGATCAGCTG
    CAGGATATTC TGGAAAGCAT GCCCACAGGT TCTGCTCCAA CTGCTGTGGC AATGGCTGGC
    ACTGATATCA ACTCTGCCTC TTGTGCTCCT TCACCTGACG ACCCTCCTCC TGTACATCTC
    TCGTCTTCAT CTTCTTCCTC TCCCTCCATT TCTCTGGAGG TGAATTCCCC AACAACAGAA
    GGTCACTCTG CCCCCCTAGC ACCTGATTTG GATGCCTATT ACCAAGATTT AGTAACTGCA
    CCTTCTGCCT CTTCTTCTCC ATCTTCTTCC CCTCCTCCAC TCCCTGCTAA ATCCCACTCA
    TCACGTAAGG CTTTGCAGAT TTCTCGCTCT CGGACAGAAA TTGAGGGTGG AGGCACTCTT
    CCACGGATGA AAACAGTCTC CCCCGCTTCT TCTCACTTTT CAGTGGCCAC TCTGGGCCGT
    AGTGTTTCCC CAAAGAATGC CCATCTGACT CAGAATGGCA CAGGCAGCCT CTCCAGGGGA
    TTGGCAGTAA CCATGCAAGA CATTCAAAGC AAGAGACAGC TGGCACTGCA ACAAAAAGGT
    CAACAAGTGA TAGAGGAGCA GCGCAGACGT CTTGCAGAGT TGAAACAGAG AGCTGCAGTT
    GAGGCACAAT CTCAGTGGGA ATCTCTCCAT GGAGCCCCTA ATCAAGGGTA CACGCCTATA
    ATTCACCACT CCATTCTACA CCATCAGCGG CCTCTCCATT CTCGTGAAGA TGACCCTACC
    CATGGATTTG ACACTCTCAG TCTGGAGAGT TCTGATAGTT TGGACACTAG TGTTTCTACA
    GGAAACTCTG CATGTTCCCC TGATAACATG TCAAGTGCTA GTGGGTTAGA CATGCTAAAG
    ATAGAGGAAA TGGAAAGAAT GCTTCTAGAA GCTCATGCAG AGCGTGCCAG GCTTGTAGAA
    TCCAGGGAGC GTGAGGTGGA GGCACGCAGA AAAGCACTAG AAGATGAGAG GCGAAGGAGA
    GAGGAGCTGG AAAGGCGTCT TCAAGATGAG ACAGCTCACA GGCAAAGACT AGTAGAGAAG
    GAGGTCAAGA TGAGGGAGAA GCATTTTTCA CAGGCTCGTC CTCTTACACG CTATCTGCCA
    ATCCGGAAGG AAGACTTTGA TCTCCGAGCC CATGTTGAGT CCTCAGGCCA TGCAGTAGAT
    GCCTGCCCTC ACATCATTTT AAGTGAGAAG ATGTGTCGTG GCTTCCTGAC AAAAATGGGT
    GGAAAGATAA AATCATGGAA GAAGCGCTGG TTTGTTTTTG ATCGCCTGAA ACGGACCCTG
    TCCTATTATG TAGATAAACA TGAGGCAAAG CTGAAAGGAG TTATATATTT CCAGGCCATA
    GAAGAGGTTT ATTATGACCA CCTCAGGAGT GCAGCCAAGA AAGGACTTTT TAACCTCAGC
    CTGGCCACTC AGATATCCGA TTTTCTTTCT GTGCCCCTGT GGAAGGAGAG TCCTAACCCA
    GCTCTAACAT TCTGTGTCAA GACTCACGAT CGCCTGTATT ACATGGTAGC TCCTTCCCCA
    GAGGCTATGC GGATATGGAT GGATGTCATT GTAACTGGGG CAGAGGGTTA CACTCAGTTC
    ATGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa45339
    Clone ID Related Accession (Same CDS sequence) XM_018095290.1
    Accession Version XM_018095290.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4278bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pleckstrin homology-like domain family B member 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA
    GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG
    CAAGAGGTGA ATTCCCCAAC AACAGAAGGT CACTCTGCCC CCCTAGCACC TGATTTGGAT
    GCCTATTACC AAGATTTAGT AACTGCACCT TCTGCCTCTT CTTCTCCATC TTCTTCCCCT
    CCTCCACTCC CTGCTAAATC CCACTCATCA CGTAAGGCTT TGCAGATTTC TCGCTCTCGG
    ACAGAAATTG AGGGTGGAGG CACTCTTCCA CGGATGAAAA CAGTCTCCCC CGCTTCTTCT
    CACTTTTCAG TGGCCACTCT GGGCCGTAGT GTTTCCCCAA AGAATGCCCA TCTGACTCAG
    AATGGCACAG GCAGCCTCTC CAGGGGATTG GCAGTAACCA TGCAAGACAT TCAAAGCAAG
    AGACAGCTGG CACTGCAACA AAAAGACAGT CTGAGCACAG AAGATTCTCT TCCATCTGAA
    GTTGAAGGTC AACAAGTGAT AGAGGAGCAG CGCAGACGTC TTGCAGAGTT GAAACAGAGA
    GCTGCAGTTG AGGCACAATC TCAGTGGGAA TCTCTCCATG GAGCCCCTAA TCAAGGGTAC
    ACGCCTATAA TTCACCACTC CATTCTACAC CATCAGCGGC CTCTCCATTC TCGTGAAGAT
    GACCCTACCC ATGGATTTGA CACTCTCAGT CTGGAGAGTT CTGATAGTTT GGACACTAGT
    GTTTCTACAG GAAACTCTGC ATGTTCCCCT GATAACATGT CAAGTGCTAG TGGGTTAGAC
    ATGCTAAAGA TAGAGGAAAT GGAAAGAATG CTTCTAGAAG CTCATGCAGA GCGTGCCAGG
    CTTGTAGAAT CCAGGGAGCG TGAGGTGGAG GCACGCAGAA AAGCACTAGA AGATGAGAGG
    CGAAGGAGAG AGGAGCTGGA AAGGCGTCTT CAAGATGAGA CAGCTCACAG GCAAAGACTA
    GTAGAGAAGG AGGTCAAGAT GAGGGAGAAG CATTTTTCAC AGGCTCGTCC TCTTACACGC
    TATCTGCCAA TCCGGAAGGA AGACTTTGAT CTCCGAGCCC ATGTTGAGTC CTCAGGCCAT
    GCAGTAGATG CCTGCCCTCA CATCATTTTA AGTGAGAAGA TGTGTCGTGG CTTCCTGACA
    AAAATGGGTG GAAAGATAAA ATCATGGAAG AAGCGCTGGT TTGTTTTTGA TCGCCTGAAA
    CGGACCCTGT CCTATTATGT AGATAAACAT GAGGCAAAGC TGAAAGGAGT TATATATTTC
    CAGGCCATAG AAGAGGTTTA TTATGACCAC CTCAGGAGTG CAGCCAAGAA AGGACTTTTT
    AACCTCAGCC TGGCCACTCA GATATCCGAT TTTCTTTCTG TGCCCCTGTG GAAGGAGAGT
    CCTAACCCAG CTCTAACATT CTGTGTCAAG ACTCACGATC GCCTGTATTA CATGGTAGCT
    CCTTCCCCAG AGGCTATGCG GATATGGATG GATGTCATTG TAACTGGGGC AGAGGGTTAC
    ACTCAGTTCA TGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017950779.1
    CDS61..4338
    Translation

    Target ORF information:

    RefSeq Version XM_018095290.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018095290.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA
    GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG
    CAAGAGGTGA ATTCCCCAAC AACAGAAGGT CACTCTGCCC CCCTAGCACC TGATTTGGAT
    GCCTATTACC AAGATTTAGT AACTGCACCT TCTGCCTCTT CTTCTCCATC TTCTTCCCCT
    CCTCCACTCC CTGCTAAATC CCACTCATCA CGTAAGGCTT TGCAGATTTC TCGCTCTCGG
    ACAGAAATTG AGGGTGGAGG CACTCTTCCA CGGATGAAAA CAGTCTCCCC CGCTTCTTCT
    CACTTTTCAG TGGCCACTCT GGGCCGTAGT GTTTCCCCAA AGAATGCCCA TCTGACTCAG
    AATGGCACAG GCAGCCTCTC CAGGGGATTG GCAGTAACCA TGCAAGACAT TCAAAGCAAG
    AGACAGCTGG CACTGCAACA AAAAGACAGT CTGAGCACAG AAGATTCTCT TCCATCTGAA
    GTTGAAGGTC AACAAGTGAT AGAGGAGCAG CGCAGACGTC TTGCAGAGTT GAAACAGAGA
    GCTGCAGTTG AGGCACAATC TCAGTGGGAA TCTCTCCATG GAGCCCCTAA TCAAGGGTAC
    ACGCCTATAA TTCACCACTC CATTCTACAC CATCAGCGGC CTCTCCATTC TCGTGAAGAT
    GACCCTACCC ATGGATTTGA CACTCTCAGT CTGGAGAGTT CTGATAGTTT GGACACTAGT
    GTTTCTACAG GAAACTCTGC ATGTTCCCCT GATAACATGT CAAGTGCTAG TGGGTTAGAC
    ATGCTAAAGA TAGAGGAAAT GGAAAGAATG CTTCTAGAAG CTCATGCAGA GCGTGCCAGG
    CTTGTAGAAT CCAGGGAGCG TGAGGTGGAG GCACGCAGAA AAGCACTAGA AGATGAGAGG
    CGAAGGAGAG AGGAGCTGGA AAGGCGTCTT CAAGATGAGA CAGCTCACAG GCAAAGACTA
    GTAGAGAAGG AGGTCAAGAT GAGGGAGAAG CATTTTTCAC AGGCTCGTCC TCTTACACGC
    TATCTGCCAA TCCGGAAGGA AGACTTTGAT CTCCGAGCCC ATGTTGAGTC CTCAGGCCAT
    GCAGTAGATG CCTGCCCTCA CATCATTTTA AGTGAGAAGA TGTGTCGTGG CTTCCTGACA
    AAAATGGGTG GAAAGATAAA ATCATGGAAG AAGCGCTGGT TTGTTTTTGA TCGCCTGAAA
    CGGACCCTGT CCTATTATGT AGATAAACAT GAGGCAAAGC TGAAAGGAGT TATATATTTC
    CAGGCCATAG AAGAGGTTTA TTATGACCAC CTCAGGAGTG CAGCCAAGAA AGGACTTTTT
    AACCTCAGCC TGGCCACTCA GATATCCGAT TTTCTTTCTG TGCCCCTGTG GAAGGAGAGT
    CCTAACCCAG CTCTAACATT CTGTGTCAAG ACTCACGATC GCCTGTATTA CATGGTAGCT
    CCTTCCCCAG AGGCTATGCG GATATGGATG GATGTCATTG TAACTGGGGC AGAGGGTTAC
    ACTCAGTTCA TGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa61273
    Clone ID Related Accession (Same CDS sequence) XM_031904870.1
    Accession Version XM_031904870.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4482bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pleckstrin homology-like domain family B member 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGATTATT TGGAAACAGA ATTCCCTGCT CAGACAGAAA TAGAGGACAC AATGAAGGCA 
    CTGAGTCATG ATGTGAGCAG CCCCTGCAGA AAAACACACA CAGTTATTCA GAGCACACCA
    TTGGATCTCA TAGATACAGG AAAAAGTCTT AGGGTGCAGA CAGAAAAACC TCATCTTGTC
    AGCCTGGGAA GTGGACGACT CAGCACTGCA ATCACCCTAC TACCACTGGA AGAAGGAAGG
    ACAACCCTGG GTCATGCATC TAAGGACATT CCCCTGCAGG GACCTGGCAT TGCTGCAGAA
    CATTGCTACA TTGAAAACAT CAGAGGAAAC CTCACCCTAT ACCCATGTGG AAATCCTTGT
    GCCATCGATG GACTGCTCAT CACCCAACCG ACACGTCTCA CTCAAGGGTG TATGGTCTGT
    CTGGGTCAGT CCACCTTCCT GAGATTTAAC CACCCAGCTG AAGCTCACTG GATGAAAACC
    ATGATTCCTT CCACTGGGAG AAGCCCTGCG CCAGGCTTGT ACCCATCAGA TTCTTCTTCC
    TCCTTGCTAA ATGGTTCTCG TGATGCTGCA TACCCGAGAA CTGTACCTTC CCATACCTCA
    CTAGTAAGCT CCATTGAAAA AAATCTTCAG GACATCATGG ACTCGTTGGT TTTAGAAGGG
    CCTGCACCAC TGAGCAGGAA GCTTTCCCAG ACCTCACTTA ACCATATCAC CCCTATGACC
    AATGGAGAGA ACAGACGGTA CTTGCTTTCA TCTCCAACAA GTCCACCTTA CTCAAACTCA
    GGTTATGACA ATACCTCTCC ACTGTCTTCT CCTGGAAGTG GAAGTCTCAC TAGCCCCTCT
    CCGGGTCAAG ATGCAGGAAC GCCAACAGCA CCCCCTGTGG TGCCACTTCG AACTTCTAGC
    TTTAGCCATT CCTTGCCACG ACCAGTTCCT ACCTATGGAG GAAGCAACCT TGACTTGCGT
    TCTAATCGCA ACACAGGTGG TGGGTCTCTG CTTAGTCCTT CAAGCCCAGC AACAGGAAGC
    AGGATGGGGG GACTAAGTGT GCCAAGCAGT CCACTCCTAG GTAGTAAGTT TCATCACCAG
    ATCACAGACC GCCCAACCAG CCCTTTTAGA CAGGTGGTAG GACCAACACG GAGTCTGCCA
    CCAGAGAGTC CAAGACTTGG GCGGAGGAAC TTTGAAAGCA TGAGGGACCT GCCTCCACTT
    AGCCCTTCTC TGCCCCGAAG AAGTTCATCT AGCTCCATTA AACCTGCTCC AGACAGCCCA
    TCTCGAGTTC CAGACAGCCC CAGCACTTTA GGGCGACGTC GGGTGGGAAG TGCTAGCTCT
    CCTGGCACAG AAAATAATGC AAGTACAAGG CAGATAAGGG AGCGCAGCCC ATCTCCCTCC
    TCTCTTCAAG AGACCTCTCG TCGACTAACT CCAGCTTATA GCCTGGGATC GCTTGGGACC
    CCTTCACAAA GCCCCAGGGC ACAAAGAAAA GGGCCTGTGC TACGGGAGCG CAAGAACAGC
    ATCAGTGAAA TTTCTGGCAA CCAAGAGGAG CTAATGGACT ACCACAGAAG GCAGAGAGAA
    GAAAGACTTA GAGAGCAGGA AATGGAAAGG CTGGAGCGGC AGAGACTGGA AACCATTTTA
    AACTTGTGTG CCGAATACAG TAAAACAGAT GATACAGCTC CCCCAGCTCG CCTAGATGAA
    ATAAGGAGCT TCCCCACTTT GGGGGGTACT GAGGAAACCA CTGCATCCCT CTTCACTCGT
    AGACCTTCTT GGGAGAGCAC CAGAAGTACC AACCTGGAGG GAACAGGCAA TAGGGTGAGA
    GCACAGGGAG AACACAATGA GGAAGAGACT CAGAGAGAAG AGAACAGCAG TACAGAGAGT
    ACCCAACATG AGCAGGAAGA TGCTGTAAGC CCAACTGGGG CTGCAGAATT ACTAGCCCTG
    GAGGAGCAAC GCAGGCACAC CCTGGGGCAT GTGGATCAGC TAAAGAATAG AGTGACTGAA
    TTGGAGGGTC AGTTGCAGGA AAGTGCAAGG GAGGCAGAGA TGGAACGTGC CCTTTTGCAA
    GGGGAACGGG AGGCTGAGCT GTCCCAGTTG CAGCATGAGC AAAGAATGGT CCAGCAGCTG
    CAGGACAGCC TGACCTCTTT AGAAAACAAC TTGCAGAAGG AGAAAGACAA GGCACGTGAG
    AAACTGGAGC AGGAGAGGAT GGAGCTGCAG AGTATTCAGC TTTCTCACCA CGACCTGCAG
    AGGCAGCTCC ATAACTGCCC TGAGGCTCTG CGGGAGCAGG TGCAGTGCCA GCTGACACGG
    GAGTCTGAAC TCCTGGAGAC GAAGATGAAG CTGTTTGAGG ATCTGGAGTT CCAGCAGCTA
    GAAAAGGAAA GCCGCCTGGA AGAAGAAAGA GAGACCACAA GTCAACAACT TTTACAGAGC
    AAGGGGGAAT GCCAGCGAAG CATTGCACGC AGGAAGGAAC GCCTGGCTGC TTTGGAGATG
    CAAGCCAATC AGATTCGCCT CCAGGCAGCA CAGGATGCAG ACCATCTTGG CCAGGAAAGA
    AGCGCCATAC TTTTACAGCT ACACAAGGAG AAAGAGAAGC TGGTTCAGTT GGAGAGAAGA
    TACCAGGCAC TAACAGGACG ACGGGGATTC TCAACTGGAT CAGCTGCTCT CCGAGAGGAT
    GCTCTTCAAA TCTATGAGAC TAGAGACTGC TTACTCGCTC AAGCTGATGT TTCTTGCACA
    CCTGCTATCC CTTCTGCCTG CTCTCACTCC CCTTCCCGGC AGCAAGAGGA ATACCTGTGC
    CTCTCTGATG TTCTCCGCTT GTACAGCAGC TCGCCTCTTG TTCCTGCTTT TCCCCTTCTC
    TCTGCTGCAG AGCCCACCGC CGCCCTTGAG TACATCACTT TAGATCAGCT GCAGGATATT
    CTGGAAAGCA TGCCCACAGG TTCTGCTCCA ACTGCTGTGG CAATGGCTGG CACTGATATC
    AACTCTGCCT CTTGTGCTCC TTCACCTGAC GACCCTCCTC CTGTACATCT CTCGTCTTCA
    TCTTCTTCCT CTCCCTCCAT TTCTCTGGAG GTGAATTCCC CAACAACAGA AGGTCACTCT
    GCCCCCCTAG CACCTGATTT GGATGCCTAT TACCAAGATT TAGTAACTGC ACCTTCTGCC
    TCTTCTTCTC CATCTTCTTC CCCTCCTCCA CTCCCTGCTA AATCCCACTC ATCACGTAAG
    GCTTTGCAGA TTTCTCGCTC TCGGACAGAA ATTGAGGGTG GAGGCACTCT TCCACGGATG
    AAAACAGTCT CCCCCGCTTC TTCTCACTTT TCAGTGGCCA CTCTGGGCCG TAGTGTTTCC
    CCAAAGAATG CCCATCTGAC TCAGAATGGC ACAGGCAGCC TCTCCAGGGG ATTGGCAGTA
    ACCATGCAAG ACATTCAAAG CAAGAGACAG CTGGCACTGC AACAAAAAGA CAGTCTGAGC
    ACAGAAGATT CTCTTCCATC TGAAGTTGAA GGTCAACAAG TGATAGAGGA GCAGCGCAGA
    CGTCTTGCAG AGTTGAAACA GAGAGCTGCA GTTGAGGCAC AATCTCAGTG GGAATCTCTC
    CATGGAGCCC CTAATCAAGG GTACACGCCT ATAATTCACC ACTCCATTCT ACACCATCAG
    CGGCCTCTCC ATTCTCGTGA AGATGACCCT ACCCATGGAT TTGACACTCT CAGTCTGGAG
    AGTTCTGATA GTTTGGACAC TAGTGTTTCT ACAGGAAACT CTGCATGTTC CCCTGATAAC
    ATGTCAAGTG CTAGTGGGTT AGACATGCTA AAGATAGAGG AAATGGAAAG AATGCTTCTA
    GAAGCTCATG CAGAGCGTGC CAGGCTTGTA GAATCCAGGG AGCGTGAGGT GGAGGCACGC
    AGAAAAGCAC TAGAAGATGA GAGGCGAAGG AGAGAGGAGC TGGAAAGGCG TCTTCAAGAT
    GAGACAGCTC ACAGGCAAAG ACTAGTAGAG AAGGAGGTCA AGATGAGGGA GAAGCATTTT
    TCACAGGCTC GTCCTCTTAC ACGCTATCTG CCAATCCGGA AGGAAGACTT TGATCTCCGA
    GCCCATGTTG AGTCCTCAGG CCATGCAGTA GATGCCTGCC CTCACATCAT TTTAAGTGAG
    AAGATGTGTC GTGGCTTCCT GACAAAAATG GGTGGAAAGA TAAAATCATG GAAGAAGCGC
    TGGTTTGTTT TTGATCGCCT GAAACGGACC CTGTCCTATT ATGTAGATAA ACATGAGGCA
    AAGCTGAAAG GAGTTATATA TTTCCAGGCC ATAGAAGAGG TTTATTATGA CCACCTCAGG
    AGTGCAGCCA AGAAAGGACT TTTTAACCTC AGCCTGGCCA CTCAGATATC CGATTTTCTT
    TCTGTGCCCC TGTGGAAGGA GAGTCCTAAC CCAGCTCTAA CATTCTGTGT CAAGACTCAC
    GATCGCCTGT ATTACATGGT AGCTCCTTCC CCAGAGGCTA TGCGGATATG GATGGATGTC
    ATTGTAACTG GGGCAGAGGG TTACACTCAG TTCATGAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031760730.1
    CDS16..4497
    Translation

    Target ORF information:

    RefSeq Version XM_031904870.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031904870.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGATTATT TGGAAACAGA ATTCCCTGCT CAGACAGAAA TAGAGGACAC AATGAAGGCA 
    CTGAGTCATG ATGTGAGCAG CCCCTGCAGA AAAACACACA CAGTTATTCA GAGCACACCA
    TTGGATCTCA TAGATACAGG AAAAAGTCTT AGGGTGCAGA CAGAAAAACC TCATCTTGTC
    AGCCTGGGAA GTGGACGACT CAGCACTGCA ATCACCCTAC TACCACTGGA AGAAGGAAGG
    ACAACCCTGG GTCATGCATC TAAGGACATT CCCCTGCAGG GACCTGGCAT TGCTGCAGAA
    CATTGCTACA TTGAAAACAT CAGAGGAAAC CTCACCCTAT ACCCATGTGG AAATCCTTGT
    GCCATCGATG GACTGCTCAT CACCCAACCG ACACGTCTCA CTCAAGGGTG TATGGTCTGT
    CTGGGTCAGT CCACCTTCCT GAGATTTAAC CACCCAGCTG AAGCTCACTG GATGAAAACC
    ATGATTCCTT CCACTGGGAG AAGCCCTGCG CCAGGCTTGT ACCCATCAGA TTCTTCTTCC
    TCCTTGCTAA ATGGTTCTCG TGATGCTGCA TACCCGAGAA CTGTACCTTC CCATACCTCA
    CTAGTAAGCT CCATTGAAAA AAATCTTCAG GACATCATGG ACTCGTTGGT TTTAGAAGGG
    CCTGCACCAC TGAGCAGGAA GCTTTCCCAG ACCTCACTTA ACCATATCAC CCCTATGACC
    AATGGAGAGA ACAGACGGTA CTTGCTTTCA TCTCCAACAA GTCCACCTTA CTCAAACTCA
    GGTTATGACA ATACCTCTCC ACTGTCTTCT CCTGGAAGTG GAAGTCTCAC TAGCCCCTCT
    CCGGGTCAAG ATGCAGGAAC GCCAACAGCA CCCCCTGTGG TGCCACTTCG AACTTCTAGC
    TTTAGCCATT CCTTGCCACG ACCAGTTCCT ACCTATGGAG GAAGCAACCT TGACTTGCGT
    TCTAATCGCA ACACAGGTGG TGGGTCTCTG CTTAGTCCTT CAAGCCCAGC AACAGGAAGC
    AGGATGGGGG GACTAAGTGT GCCAAGCAGT CCACTCCTAG GTAGTAAGTT TCATCACCAG
    ATCACAGACC GCCCAACCAG CCCTTTTAGA CAGGTGGTAG GACCAACACG GAGTCTGCCA
    CCAGAGAGTC CAAGACTTGG GCGGAGGAAC TTTGAAAGCA TGAGGGACCT GCCTCCACTT
    AGCCCTTCTC TGCCCCGAAG AAGTTCATCT AGCTCCATTA AACCTGCTCC AGACAGCCCA
    TCTCGAGTTC CAGACAGCCC CAGCACTTTA GGGCGACGTC GGGTGGGAAG TGCTAGCTCT
    CCTGGCACAG AAAATAATGC AAGTACAAGG CAGATAAGGG AGCGCAGCCC ATCTCCCTCC
    TCTCTTCAAG AGACCTCTCG TCGACTAACT CCAGCTTATA GCCTGGGATC GCTTGGGACC
    CCTTCACAAA GCCCCAGGGC ACAAAGAAAA GGGCCTGTGC TACGGGAGCG CAAGAACAGC
    ATCAGTGAAA TTTCTGGCAA CCAAGAGGAG CTAATGGACT ACCACAGAAG GCAGAGAGAA
    GAAAGACTTA GAGAGCAGGA AATGGAAAGG CTGGAGCGGC AGAGACTGGA AACCATTTTA
    AACTTGTGTG CCGAATACAG TAAAACAGAT GATACAGCTC CCCCAGCTCG CCTAGATGAA
    ATAAGGAGCT TCCCCACTTT GGGGGGTACT GAGGAAACCA CTGCATCCCT CTTCACTCGT
    AGACCTTCTT GGGAGAGCAC CAGAAGTACC AACCTGGAGG GAACAGGCAA TAGGGTGAGA
    GCACAGGGAG AACACAATGA GGAAGAGACT CAGAGAGAAG AGAACAGCAG TACAGAGAGT
    ACCCAACATG AGCAGGAAGA TGCTGTAAGC CCAACTGGGG CTGCAGAATT ACTAGCCCTG
    GAGGAGCAAC GCAGGCACAC CCTGGGGCAT GTGGATCAGC TAAAGAATAG AGTGACTGAA
    TTGGAGGGTC AGTTGCAGGA AAGTGCAAGG GAGGCAGAGA TGGAACGTGC CCTTTTGCAA
    GGGGAACGGG AGGCTGAGCT GTCCCAGTTG CAGCATGAGC AAAGAATGGT CCAGCAGCTG
    CAGGACAGCC TGACCTCTTT AGAAAACAAC TTGCAGAAGG AGAAAGACAA GGCACGTGAG
    AAACTGGAGC AGGAGAGGAT GGAGCTGCAG AGTATTCAGC TTTCTCACCA CGACCTGCAG
    AGGCAGCTCC ATAACTGCCC TGAGGCTCTG CGGGAGCAGG TGCAGTGCCA GCTGACACGG
    GAGTCTGAAC TCCTGGAGAC GAAGATGAAG CTGTTTGAGG ATCTGGAGTT CCAGCAGCTA
    GAAAAGGAAA GCCGCCTGGA AGAAGAAAGA GAGACCACAA GTCAACAACT TTTACAGAGC
    AAGGGGGAAT GCCAGCGAAG CATTGCACGC AGGAAGGAAC GCCTGGCTGC TTTGGAGATG
    CAAGCCAATC AGATTCGCCT CCAGGCAGCA CAGGATGCAG ACCATCTTGG CCAGGAAAGA
    AGCGCCATAC TTTTACAGCT ACACAAGGAG AAAGAGAAGC TGGTTCAGTT GGAGAGAAGA
    TACCAGGCAC TAACAGGACG ACGGGGATTC TCAACTGGAT CAGCTGCTCT CCGAGAGGAT
    GCTCTTCAAA TCTATGAGAC TAGAGACTGC TTACTCGCTC AAGCTGATGT TTCTTGCACA
    CCTGCTATCC CTTCTGCCTG CTCTCACTCC CCTTCCCGGC AGCAAGAGGA ATACCTGTGC
    CTCTCTGATG TTCTCCGCTT GTACAGCAGC TCGCCTCTTG TTCCTGCTTT TCCCCTTCTC
    TCTGCTGCAG AGCCCACCGC CGCCCTTGAG TACATCACTT TAGATCAGCT GCAGGATATT
    CTGGAAAGCA TGCCCACAGG TTCTGCTCCA ACTGCTGTGG CAATGGCTGG CACTGATATC
    AACTCTGCCT CTTGTGCTCC TTCACCTGAC GACCCTCCTC CTGTACATCT CTCGTCTTCA
    TCTTCTTCCT CTCCCTCCAT TTCTCTGGAG GTGAATTCCC CAACAACAGA AGGTCACTCT
    GCCCCCCTAG CACCTGATTT GGATGCCTAT TACCAAGATT TAGTAACTGC ACCTTCTGCC
    TCTTCTTCTC CATCTTCTTC CCCTCCTCCA CTCCCTGCTA AATCCCACTC ATCACGTAAG
    GCTTTGCAGA TTTCTCGCTC TCGGACAGAA ATTGAGGGTG GAGGCACTCT TCCACGGATG
    AAAACAGTCT CCCCCGCTTC TTCTCACTTT TCAGTGGCCA CTCTGGGCCG TAGTGTTTCC
    CCAAAGAATG CCCATCTGAC TCAGAATGGC ACAGGCAGCC TCTCCAGGGG ATTGGCAGTA
    ACCATGCAAG ACATTCAAAG CAAGAGACAG CTGGCACTGC AACAAAAAGA CAGTCTGAGC
    ACAGAAGATT CTCTTCCATC TGAAGTTGAA GGTCAACAAG TGATAGAGGA GCAGCGCAGA
    CGTCTTGCAG AGTTGAAACA GAGAGCTGCA GTTGAGGCAC AATCTCAGTG GGAATCTCTC
    CATGGAGCCC CTAATCAAGG GTACACGCCT ATAATTCACC ACTCCATTCT ACACCATCAG
    CGGCCTCTCC ATTCTCGTGA AGATGACCCT ACCCATGGAT TTGACACTCT CAGTCTGGAG
    AGTTCTGATA GTTTGGACAC TAGTGTTTCT ACAGGAAACT CTGCATGTTC CCCTGATAAC
    ATGTCAAGTG CTAGTGGGTT AGACATGCTA AAGATAGAGG AAATGGAAAG AATGCTTCTA
    GAAGCTCATG CAGAGCGTGC CAGGCTTGTA GAATCCAGGG AGCGTGAGGT GGAGGCACGC
    AGAAAAGCAC TAGAAGATGA GAGGCGAAGG AGAGAGGAGC TGGAAAGGCG TCTTCAAGAT
    GAGACAGCTC ACAGGCAAAG ACTAGTAGAG AAGGAGGTCA AGATGAGGGA GAAGCATTTT
    TCACAGGCTC GTCCTCTTAC ACGCTATCTG CCAATCCGGA AGGAAGACTT TGATCTCCGA
    GCCCATGTTG AGTCCTCAGG CCATGCAGTA GATGCCTGCC CTCACATCAT TTTAAGTGAG
    AAGATGTGTC GTGGCTTCCT GACAAAAATG GGTGGAAAGA TAAAATCATG GAAGAAGCGC
    TGGTTTGTTT TTGATCGCCT GAAACGGACC CTGTCCTATT ATGTAGATAA ACATGAGGCA
    AAGCTGAAAG GAGTTATATA TTTCCAGGCC ATAGAAGAGG TTTATTATGA CCACCTCAGG
    AGTGCAGCCA AGAAAGGACT TTTTAACCTC AGCCTGGCCA CTCAGATATC CGATTTTCTT
    TCTGTGCCCC TGTGGAAGGA GAGTCCTAAC CCAGCTCTAA CATTCTGTGT CAAGACTCAC
    GATCGCCTGT ATTACATGGT AGCTCCTTCC CCAGAGGCTA TGCGGATATG GATGGATGTC
    ATTGTAACTG GGGCAGAGGG TTACACTCAG TTCATGAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa61274
    Clone ID Related Accession (Same CDS sequence) XM_031904871.1
    Accession Version XM_031904871.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4347bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pleckstrin homology-like domain family B member 1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGTACAT CACTTTAGAT CAGCTGCAGG ATATTCTGGA AAGCATGCCC
    ACAGGTTCTG CTCCAACTGC TGTGGCAATG GCTGGCACTG ATATCAACTC TGCCTCTTGT
    GCTCCTTCAC CTGACGACCC TCCTCCTGTA CATCTCTCGT CTTCATCTTC TTCCTCTCCC
    TCCATTTCTC TGGAGGTGAA TTCCCCAACA ACAGAAGGTC ACTCTGCCCC CCTAGCACCT
    GATTTGGATG CCTATTACCA AGATTTAGTA ACTGCACCTT CTGCCTCTTC TTCTCCATCT
    TCTTCCCCTC CTCCACTCCC TGCTAAATCC CACTCATCAC GTAAGGCTTT GCAGATTTCT
    CGCTCTCGGA CAGAAATTGA GGGTGGAGGC ACTCTTCCAC GGATGAAAAC AGTCTCCCCC
    GCTTCTTCTC ACTTTTCAGT GGCCACTCTG GGCCGTAGTG TTTCCCCAAA GAATGCCCAT
    CTGACTCAGA ATGGCACAGG CAGCCTCTCC AGGGGATTGG CAGTAACCAT GCAAGACATT
    CAAAGCAAGA GACAGCTGGC ACTGCAACAA AAAGACAGTC TGAGCACAGA AGATTCTCTT
    CCATCTGAAG TTGAAGGTCA ACAAGTGATA GAGGAGCAGC GCAGACGTCT TGCAGAGTTG
    AAACAGAGAG CTGCAGTTGA GGCACAATCT CAGTGGGAAT CTCTCCATGG AGCCCCTAAT
    CAAGGGTACA CGCCTATAAT TCACCACTCC ATTCTACACC ATCAGCGGCC TCTCCATTCT
    CGTGAAGATG ACCCTACCCA TGGATTTGAC ACTCTCAGTC TGGAGAGTTC TGATAGTTTG
    GACACTAGTG TTTCTACAGG AAACTCTGCA TGTTCCCCTG ATAACATGTC AAGTGCTAGT
    GGGTTAGACA TGCTAAAGAT AGAGGAAATG GAAAGAATGC TTCTAGAAGC TCATGCAGAG
    CGTGCCAGGC TTGTAGAATC CAGGGAGCGT GAGGTGGAGG CACGCAGAAA AGCACTAGAA
    GATGAGAGGC GAAGGAGAGA GGAGCTGGAA AGGCGTCTTC AAGATGAGAC AGCTCACAGG
    CAAAGACTAG TAGAGAAGGA GGTCAAGATG AGGGAGAAGC ATTTTTCACA GGCTCGTCCT
    CTTACACGCT ATCTGCCAAT CCGGAAGGAA GACTTTGATC TCCGAGCCCA TGTTGAGTCC
    TCAGGCCATG CAGTAGATGC CTGCCCTCAC ATCATTTTAA GTGAGAAGAT GTGTCGTGGC
    TTCCTGACAA AAATGGGTGG AAAGATAAAA TCATGGAAGA AGCGCTGGTT TGTTTTTGAT
    CGCCTGAAAC GGACCCTGTC CTATTATGTA GATAAACATG AGGCAAAGCT GAAAGGAGTT
    ATATATTTCC AGGCCATAGA AGAGGTTTAT TATGACCACC TCAGGAGTGC AGCCAAGAAA
    GGACTTTTTA ACCTCAGCCT GGCCACTCAG ATATCCGATT TTCTTTCTGT GCCCCTGTGG
    AAGGAGAGTC CTAACCCAGC TCTAACATTC TGTGTCAAGA CTCACGATCG CCTGTATTAC
    ATGGTAGCTC CTTCCCCAGA GGCTATGCGG ATATGGATGG ATGTCATTGT AACTGGGGCA
    GAGGGTTACA CTCAGTTCAT GAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031760731.1
    CDS35..4381
    Translation

    Target ORF information:

    RefSeq Version XM_031904871.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031904871.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGTACAT CACTTTAGAT CAGCTGCAGG ATATTCTGGA AAGCATGCCC
    ACAGGTTCTG CTCCAACTGC TGTGGCAATG GCTGGCACTG ATATCAACTC TGCCTCTTGT
    GCTCCTTCAC CTGACGACCC TCCTCCTGTA CATCTCTCGT CTTCATCTTC TTCCTCTCCC
    TCCATTTCTC TGGAGGTGAA TTCCCCAACA ACAGAAGGTC ACTCTGCCCC CCTAGCACCT
    GATTTGGATG CCTATTACCA AGATTTAGTA ACTGCACCTT CTGCCTCTTC TTCTCCATCT
    TCTTCCCCTC CTCCACTCCC TGCTAAATCC CACTCATCAC GTAAGGCTTT GCAGATTTCT
    CGCTCTCGGA CAGAAATTGA GGGTGGAGGC ACTCTTCCAC GGATGAAAAC AGTCTCCCCC
    GCTTCTTCTC ACTTTTCAGT GGCCACTCTG GGCCGTAGTG TTTCCCCAAA GAATGCCCAT
    CTGACTCAGA ATGGCACAGG CAGCCTCTCC AGGGGATTGG CAGTAACCAT GCAAGACATT
    CAAAGCAAGA GACAGCTGGC ACTGCAACAA AAAGACAGTC TGAGCACAGA AGATTCTCTT
    CCATCTGAAG TTGAAGGTCA ACAAGTGATA GAGGAGCAGC GCAGACGTCT TGCAGAGTTG
    AAACAGAGAG CTGCAGTTGA GGCACAATCT CAGTGGGAAT CTCTCCATGG AGCCCCTAAT
    CAAGGGTACA CGCCTATAAT TCACCACTCC ATTCTACACC ATCAGCGGCC TCTCCATTCT
    CGTGAAGATG ACCCTACCCA TGGATTTGAC ACTCTCAGTC TGGAGAGTTC TGATAGTTTG
    GACACTAGTG TTTCTACAGG AAACTCTGCA TGTTCCCCTG ATAACATGTC AAGTGCTAGT
    GGGTTAGACA TGCTAAAGAT AGAGGAAATG GAAAGAATGC TTCTAGAAGC TCATGCAGAG
    CGTGCCAGGC TTGTAGAATC CAGGGAGCGT GAGGTGGAGG CACGCAGAAA AGCACTAGAA
    GATGAGAGGC GAAGGAGAGA GGAGCTGGAA AGGCGTCTTC AAGATGAGAC AGCTCACAGG
    CAAAGACTAG TAGAGAAGGA GGTCAAGATG AGGGAGAAGC ATTTTTCACA GGCTCGTCCT
    CTTACACGCT ATCTGCCAAT CCGGAAGGAA GACTTTGATC TCCGAGCCCA TGTTGAGTCC
    TCAGGCCATG CAGTAGATGC CTGCCCTCAC ATCATTTTAA GTGAGAAGAT GTGTCGTGGC
    TTCCTGACAA AAATGGGTGG AAAGATAAAA TCATGGAAGA AGCGCTGGTT TGTTTTTGAT
    CGCCTGAAAC GGACCCTGTC CTATTATGTA GATAAACATG AGGCAAAGCT GAAAGGAGTT
    ATATATTTCC AGGCCATAGA AGAGGTTTAT TATGACCACC TCAGGAGTGC AGCCAAGAAA
    GGACTTTTTA ACCTCAGCCT GGCCACTCAG ATATCCGATT TTCTTTCTGT GCCCCTGTGG
    AAGGAGAGTC CTAACCCAGC TCTAACATTC TGTGTCAAGA CTCACGATCG CCTGTATTAC
    ATGGTAGCTC CTTCCCCAGA GGCTATGCGG ATATGGATGG ATGTCATTGT AACTGGGGCA
    GAGGGTTACA CTCAGTTCAT GAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa61275
    Clone ID Related Accession (Same CDS sequence) XM_031904872.1
    Accession Version XM_031904872.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4233bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pleckstrin homology-like domain family B member 1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG GAAGATGCTG TAAGCCCAAC TGGGGCTGCA
    GAATTACTAG CCCTGGAGGA GCAACGCAGG CACACCCTGG GGCATGTGGA TCAGCTAAAG
    AATAGAGTGA CTGAATTGGA GGGTCAGTTG CAGGAAAGTG CAAGGGAGGC AGAGATGGAA
    CGTGCCCTTT TGCAAGGGGA ACGGGAGGCT GAGCTGTCCC AGTTGCAGCA TGAGCAAAGA
    ATGGTCCAGC AGCTGCAGGA CAGCCTGACC TCTTTAGAAA ACAACTTGCA GAAGGAGAAA
    GACAAGGCAC GTGAGAAACT GGAGCAGGAG AGGATGGAGC TGCAGAGTAT TCAGCTTTCT
    CACCACGACC TGCAGAGGCA GCTCCATAAC TGCCCTGAGG CTCTGCGGGA GCAGGTGCAG
    TGCCAGCTGA CACGGGAGTC TGAACTCCTG GAGACGAAGA TGAAGCTGTT TGAGGATCTG
    GAGTTCCAGC AGCTAGAAAA GGAAAGCCGC CTGGAAGAAG AAAGAGAGAC CACAAGTCAA
    CAACTTTTAC AGAGCAAGGG GGAATGCCAG CGAAGCATTG CACGCAGGAA GGAACGCCTG
    GCTGCTTTGG AGATGCAAGC CAATCAGATT CGCCTCCAGG CAGCACAGGA TGCAGACCAT
    CTTGGCCAGG AAAGAAGCGC CATACTTTTA CAGCTACACA AGGAGAAAGA GAAGCTGGTT
    CAGTTGGAGA GAAGATACCA GGCACTAACA GGACGACGGG GATTCTCAAC TGGATCAGCT
    GCTCTCCGAG AGTACATCAC TTTAGATCAG CTGCAGGATA TTCTGGAAAG CATGCCCACA
    GGTTCTGCTC CAACTGCTGT GGCAATGGCT GGCACTGATA TCAACTCTGC CTCTTGTGCT
    CCTTCACCTG ACGACCCTCC TCCTGTACAT CTCTCGTCTT CATCTTCTTC CTCTCCCTCC
    ATTTCTCTGG AGGTGAATTC CCCAACAACA GAAGGTCACT CTGCCCCCCT AGCACCTGAT
    TTGGATGCCT ATTACCAAGA TTTAGTAACT GCACCTTCTG CCTCTTCTTC TCCATCTTCT
    TCCCCTCCTC CACTCCCTGC TAAATCCCAC TCATCACGTA AGGCTTTGCA GATTTCTCGC
    TCTCGGACAG AAATTGAGGG TGGAGGCACT CTTCCACGGA TGAAAACAGT CTCCCCCGCT
    TCTTCTCACT TTTCAGTGGC CACTCTGGGC CGTAGTGTTT CCCCAAAGAA TGCCCATCTG
    ACTCAGAATG GCACAGGCAG CCTCTCCAGG GGATTGGCAG TAACCATGCA AGACATTCAA
    AGCAAGAGAC AGCTGGCACT GCAACAAAAA GGTCAACAAG TGATAGAGGA GCAGCGCAGA
    CGTCTTGCAG AGTTGAAACA GAGAGCTGCA GTTGAGGCAC AATCTCAGTG GGAATCTCTC
    CATGGAGCCC CTAATCAAGG GTACACGCCT ATAATTCACC ACTCCATTCT ACACCATCAG
    CGGCCTCTCC ATTCTCGTGA AGATGACCCT ACCCATGGAT TTGACACTCT CAGTCTGGAG
    AGTTCTGATA GTTTGGACAC TAGTGTTTCT ACAGGAAACT CTGCATGTTC CCCTGATAAC
    ATGTCAAGTG CTAGTGGGTT AGACATGCTA AAGATAGAGG AAATGGAAAG AATGCTTCTA
    GAAGCTCATG CAGAGCGTGC CAGGCTTGTA GAATCCAGGG AGCGTGAGGT GGAGGCACGC
    AGAAAAGCAC TAGAAGATGA GAGGCGAAGG AGAGAGGAGC TGGAAAGGCG TCTTCAAGAT
    GAGACAGCTC ACAGGCAAAG ACTAGTAGAG AAGGAGGTCA AGATGAGGGA GAAGCATTTT
    TCACAGGCTC GTCCTCTTAC ACGCTATCTG CCAATCCGGA AGGAAGACTT TGATCTCCGA
    GCCCATGTTG AGTCCTCAGG CCATGCAGTA GATGCCTGCC CTCACATCAT TTTAAGTGAG
    AAGATGTGTC GTGGCTTCCT GACAAAAATG GGTGGAAAGA TAAAATCATG GAAGAAGCGC
    TGGTTTGTTT TTGATCGCCT GAAACGGACC CTGTCCTATT ATGTAGATAA ACATGAGGCA
    AAGCTGAAAG GAGTTATATA TTTCCAGGCC ATAGAAGAGG TTTATTATGA CCACCTCAGG
    AGTGCAGCCA AGAGTCCTAA CCCAGCTCTA ACATTCTGTG TCAAGACTCA CGATCGCCTG
    TATTACATGG TAGCTCCTTC CCCAGAGGCT ATGCGGATAT GGATGGATGT CATTGTAACT
    GGGGCAGAGG GTTACACTCA GTTCATGAAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031760732.1
    CDS35..4267
    Translation

    Target ORF information:

    RefSeq Version XM_031904872.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031904872.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG GAAGATGCTG TAAGCCCAAC TGGGGCTGCA
    GAATTACTAG CCCTGGAGGA GCAACGCAGG CACACCCTGG GGCATGTGGA TCAGCTAAAG
    AATAGAGTGA CTGAATTGGA GGGTCAGTTG CAGGAAAGTG CAAGGGAGGC AGAGATGGAA
    CGTGCCCTTT TGCAAGGGGA ACGGGAGGCT GAGCTGTCCC AGTTGCAGCA TGAGCAAAGA
    ATGGTCCAGC AGCTGCAGGA CAGCCTGACC TCTTTAGAAA ACAACTTGCA GAAGGAGAAA
    GACAAGGCAC GTGAGAAACT GGAGCAGGAG AGGATGGAGC TGCAGAGTAT TCAGCTTTCT
    CACCACGACC TGCAGAGGCA GCTCCATAAC TGCCCTGAGG CTCTGCGGGA GCAGGTGCAG
    TGCCAGCTGA CACGGGAGTC TGAACTCCTG GAGACGAAGA TGAAGCTGTT TGAGGATCTG
    GAGTTCCAGC AGCTAGAAAA GGAAAGCCGC CTGGAAGAAG AAAGAGAGAC CACAAGTCAA
    CAACTTTTAC AGAGCAAGGG GGAATGCCAG CGAAGCATTG CACGCAGGAA GGAACGCCTG
    GCTGCTTTGG AGATGCAAGC CAATCAGATT CGCCTCCAGG CAGCACAGGA TGCAGACCAT
    CTTGGCCAGG AAAGAAGCGC CATACTTTTA CAGCTACACA AGGAGAAAGA GAAGCTGGTT
    CAGTTGGAGA GAAGATACCA GGCACTAACA GGACGACGGG GATTCTCAAC TGGATCAGCT
    GCTCTCCGAG AGTACATCAC TTTAGATCAG CTGCAGGATA TTCTGGAAAG CATGCCCACA
    GGTTCTGCTC CAACTGCTGT GGCAATGGCT GGCACTGATA TCAACTCTGC CTCTTGTGCT
    CCTTCACCTG ACGACCCTCC TCCTGTACAT CTCTCGTCTT CATCTTCTTC CTCTCCCTCC
    ATTTCTCTGG AGGTGAATTC CCCAACAACA GAAGGTCACT CTGCCCCCCT AGCACCTGAT
    TTGGATGCCT ATTACCAAGA TTTAGTAACT GCACCTTCTG CCTCTTCTTC TCCATCTTCT
    TCCCCTCCTC CACTCCCTGC TAAATCCCAC TCATCACGTA AGGCTTTGCA GATTTCTCGC
    TCTCGGACAG AAATTGAGGG TGGAGGCACT CTTCCACGGA TGAAAACAGT CTCCCCCGCT
    TCTTCTCACT TTTCAGTGGC CACTCTGGGC CGTAGTGTTT CCCCAAAGAA TGCCCATCTG
    ACTCAGAATG GCACAGGCAG CCTCTCCAGG GGATTGGCAG TAACCATGCA AGACATTCAA
    AGCAAGAGAC AGCTGGCACT GCAACAAAAA GGTCAACAAG TGATAGAGGA GCAGCGCAGA
    CGTCTTGCAG AGTTGAAACA GAGAGCTGCA GTTGAGGCAC AATCTCAGTG GGAATCTCTC
    CATGGAGCCC CTAATCAAGG GTACACGCCT ATAATTCACC ACTCCATTCT ACACCATCAG
    CGGCCTCTCC ATTCTCGTGA AGATGACCCT ACCCATGGAT TTGACACTCT CAGTCTGGAG
    AGTTCTGATA GTTTGGACAC TAGTGTTTCT ACAGGAAACT CTGCATGTTC CCCTGATAAC
    ATGTCAAGTG CTAGTGGGTT AGACATGCTA AAGATAGAGG AAATGGAAAG AATGCTTCTA
    GAAGCTCATG CAGAGCGTGC CAGGCTTGTA GAATCCAGGG AGCGTGAGGT GGAGGCACGC
    AGAAAAGCAC TAGAAGATGA GAGGCGAAGG AGAGAGGAGC TGGAAAGGCG TCTTCAAGAT
    GAGACAGCTC ACAGGCAAAG ACTAGTAGAG AAGGAGGTCA AGATGAGGGA GAAGCATTTT
    TCACAGGCTC GTCCTCTTAC ACGCTATCTG CCAATCCGGA AGGAAGACTT TGATCTCCGA
    GCCCATGTTG AGTCCTCAGG CCATGCAGTA GATGCCTGCC CTCACATCAT TTTAAGTGAG
    AAGATGTGTC GTGGCTTCCT GACAAAAATG GGTGGAAAGA TAAAATCATG GAAGAAGCGC
    TGGTTTGTTT TTGATCGCCT GAAACGGACC CTGTCCTATT ATGTAGATAA ACATGAGGCA
    AAGCTGAAAG GAGTTATATA TTTCCAGGCC ATAGAAGAGG TTTATTATGA CCACCTCAGG
    AGTGCAGCCA AGAGTCCTAA CCCAGCTCTA ACATTCTGTG TCAAGACTCA CGATCGCCTG
    TATTACATGG TAGCTCCTTC CCCAGAGGCT ATGCGGATAT GGATGGATGT CATTGTAACT
    GGGGCAGAGG GTTACACTCA GTTCATGAAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa61276
    Clone ID Related Accession (Same CDS sequence) XM_031904873.1
    Accession Version XM_031904873.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4218bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pleckstrin homology-like domain family B member 1 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCTTCCTC TCCTCCCTCT CTTCCCCTCG CCACATCCTG CCCAAATTAC AGAAAAAGGC 
    AGAGCAGATT ATTTGGAAAC AGAATTCCCT GCTCAGACAG AAATAGAGGA CACAATGAAG
    GCACTGAGTC ATGATGTGAG CAGCCCCTGC AGAAAAACAC ACACAGTTAT TCAGAGCACA
    CCATTGGATC TCATAGATAC AGGAAAAAGT CTTAGGGTGC AGACAGAAAA ACCTCATCTT
    GTCAGCCTGG GAAGTGGACG ACTCAGCACT GCAATCACCC TACTACCACT GGAAGAAGGA
    AGGACAACCC TGGGTCATGC ATCTAAGGAC ATTCCCCTGC AGGGACCTGG CATTGCTGCA
    GAACATTGCT ACATTGAAAA CATCAGAGGA AACCTCACCC TATACCCATG TGGAAATCCT
    TGTGCCATCG ATGGACTGCT CATCACCCAA CCGACACGTC TCACTCAAGG GTGTATGGTC
    TGTCTGGGTC AGTCCACCTT CCTGAGATTT AACCACCCAG CTGAAGCTCA CTGGATGAAA
    ACCATGATTC CTTCCACTGG GAGAAGCCCT GCGCCAGGCT TGTACCCATC AGATTCTTCT
    TCCTCCTTGC TAAATGGTTC TCGTGATGCT GCATACCCGA GAACTGTACC TTCCCATACC
    TCACTAGTAA GCTCCATTGA AAAAAATCTT CAGGACATCA TGGACTCGTT GGTTTTAGAA
    GGGCCTGCAC CACTGAGCAG GAAGCTTTCC CAGACCTCAC TTAACCATAT CACCCCTATG
    ACCAATGGAG AGAACAGACG GTACTTGCTT TCATCTCCAA CAAGTCCACC TTACTCAAAC
    TCAGGTTATG ACAATACCTC TCCACTGTCT TCTCCTGGAA GTGGAAGTCT CACTAGCCCC
    TCTCCGGGTC AAGATGCAGG AACGCCAACA GCACCCCCTG TGGTGCCACT TCGAACTTCT
    AGCTTTAGCC ATTCCTTGCC ACGACCAGTT CCTACCTATG GAGGAAGCAA CCTTGACTTG
    CGTTCTAATC GCAACACAGG TGGTGGGTCT CTGCTTAGTC CTTCAAGCCC AGCAACAGGA
    AGCAGGATGG GGGGACTAAG TGTGCCAAGC AGTCCACTCC TAGGTAGTAA GTTTCATCAC
    CAGATCACAG ACCGCCCAAC CAGCCCTTTT AGACAGGTGG TAGGACCAAC ACGGAGTCTG
    CCACCAGAGA GTCCAAGACT TGGGCGGAGG AACTTTGAAA GCATGAGGGA CCTGCCTCCA
    CTTAGCCCTT CTCTGCCCCG AAGAAGTTCA TCTAGCTCCA TTAAACCTGC TCCAGACAGC
    CCATCTCGAG TTCCAGACAG CCCCAGCACT TTAGGGCGAC GTCGGGTGGG AAGTGCTAGC
    TCTCCTGGCA CAGAAAATAA TGCAAGTACA AGGCAGATAA GGGAGCGCAG CCCATCTCCC
    TCCTCTCTTC AAGAGACCTC TCGTCGACTA ACTCCAGCTT ATAGCCTGGG ATCGCTTGGG
    ACCCCTTCAC AAAGCCCCAG GGCACAAAGA AAAGGGCCTG TGCTACGGGA GCGCAAGAAC
    AGCATCAGTG AAATTTCTGG CAACCAAGAG GAGCTAATGG ACTACCACAG AAGGCAGAGA
    GAAGAAAGAC TTAGAGAGCA GGAAATGGAA AGGCTGGAGC GGCAGAGACT GGAAACCATT
    TTAAACTTGT GTGCCGAATA CAGTAAAACA GATGATACAG CTCCCCCAGC TCGCCTAGAT
    GAAATAAGGA GCTTCCCCAC TTTGGGGGGT ACTGAGGAAA CCACTGCATC CCTCTTCACT
    CGTAGACCTT CTTGGGAGAG CACCAGAAGT ACCAACCTGG AGGGAACAGG CAATAGGGTG
    AGAGCACAGG GAGAACACAA TGAGGAAGAG ACTCAGAGAG AAGAGAACAG CAGTACAGAG
    AGTACCCAAC ATGAGGAAGA TGCTGTAAGC CCAACTGGGG CTGCAGAATT ACTAGCCCTG
    GAGGAGCAAC GCAGGCACAC CCTGGGGCAT GTGGATCAGC TAAAGAATAG AGTGACTGAA
    TTGGAGGGTC AGTTGCAGGA AAGTGCAAGG GAGGCAGAGA TGGAACGTGC CCTTTTGCAA
    GGGGAACGGG AGGCTGAGCT GTCCCAGTTG CAGCATGAGC AAAGAATGGT CCAGCAGCTG
    CAGGACAGCC TGACCTCTTT AGAAAACAAC TTGCAGAAGG AGAAAGACAA GGCACGTGAG
    AAACTGGAGC AGGAGAGGAT GGAGCTGCAG AGTATTCAGC TTTCTCACCA CGACCTGCAG
    AGGCAGCTCC ATAACTGCCC TGAGGCTCTG CGGGAGCAGG TGCAGTGCCA GCTGACACGG
    GAGTCTGAAC TCCTGGAGAC GAAGATGAAG CTGTTTGAGG ATCTGGAGTT CCAGCAGCTA
    GAAAAGGAAA GCCGCCTGGA AGAAGAAAGA GAGACCACAA GTCAACAACT TTTACAGAGC
    AAGGGGGAAT GCCAGCGAAG CATTGCACGC AGGAAGGAAC GCCTGGCTGC TTTGGAGATG
    CAAGCCAATC AGATTCGCCT CCAGGCAGCA CAGGATGCAG ACCATCTTGG CCAGGAAAGA
    AGCGCCATAC TTTTACAGCT ACACAAGGAG AAAGAGAAGC TGGTTCAGTT GGAGAGAAGA
    TACCAGGCAC TAACAGGACG ACGGGGATTC TCAACTGGAT CAGCTGCTCT CCGAGAGTAC
    ATCACTTTAG ATCAGCTGCA GGATATTCTG GAAAGCATGC CCACAGGTTC TGCTCCAACT
    GCTGTGGCAA TGGCTGGCAC TGATATCAAC TCTGCCTCTT GTGCTCCTTC ACCTGACGAC
    CCTCCTCCTG TACATCTCTC GTCTTCATCT TCTTCCTCTC CCTCCATTTC TCTGGAGGTG
    AATTCCCCAA CAACAGAAGG TCACTCTGCC CCCCTAGCAC CTGATTTGGA TGCCTATTAC
    CAAGATTTAG TAACTGCACC TTCTGCCTCT TCTTCTCCAT CTTCTTCCCC TCCTCCACTC
    CCTGCTAAAT CCCACTCATC ACGTAAGGCT TTGCAGATTT CTCGCTCTCG GACAGAAATT
    GAGGGTGGAG GCACTCTTCC ACGGATGAAA ACAGTCTCCC CCGCTTCTTC TCACTTTTCA
    GTGGCCACTC TGGGCCGTAG TGTTTCCCCA AAGAATGCCC ATCTGACTCA GAATGGCACA
    GGCAGCCTCT CCAGGGGATT GGCAGTAACC ATGCAAGACA TTCAAAGCAA GAGACAGCTG
    GCACTGCAAC AAAAAGGTCA ACAAGTGATA GAGGAGCAGC GCAGACGTCT TGCAGAGTTG
    AAACAGAGAG CTGCAGTTGA GGCACAATCT CAGTGGGAAT CTCTCCATGG AGCCCCTAAT
    CAAGGGTACA CGCCTATAAT TCACCACTCC ATTCTACACC ATCAGCGGCC TCTCCATTCT
    CGTGAAGATG ACCCTACCCA TGGATTTGAC ACTCTCAGTC TGGAGAGTTC TGATAGTTTG
    GACACTAGTG TTTCTACAGG AAACTCTGCA TGTTCCCCTG ATAACATGTC AAGTGCTAGT
    GGGTTAGACA TGCTAAAGAT AGAGGAAATG GAAAGAATGC TTCTAGAAGC TCATGCAGAG
    CGTGCCAGGC TTGTAGAATC CAGGGAGCGT GAGGTGGAGG CACGCAGAAA AGCACTAGAA
    GATGAGAGGC GAAGGAGAGA GGAGCTGGAA AGGCGTCTTC AAGATGAGAC AGCTCACAGG
    CAAAGACTAG TAGAGAAGGA GGTCAAGATG AGGGAGAAGC ATTTTTCACA GGCTCGTCCT
    CTTACACGCT ATCTGCCAAT CCGGAAGGAA GACTTTGATC TCCGAGCCCA TGTTGAGTCC
    TCAGGCCATG CAGTAGATGC CTGCCCTCAC ATCATTTTAA GTGAGAAGAT GTGTCGTGGC
    TTCCTGACAA AAATGGGTGG AAAGATAAAA TCATGGAAGA AGCGCTGGTT TGTTTTTGAT
    CGCCTGAAAC GGACCCTGTC CTATTATGTA GATAAACATG AGGCAAAGCT GAAAGGAGTT
    ATATATTTCC AGGCCATAGA AGAGGTTTAT TATGACCACC TCAGGAGTGC AGCCAAGAGT
    CCTAACCCAG CTCTAACATT CTGTGTCAAG ACTCACGATC GCCTGTATTA CATGGTAGCT
    CCTTCCCCAG AGGCTATGCG GATATGGATG GATGTCATTG TAACTGGGGC AGAGGGTTAC
    ACTCAGTTCA TGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031760733.1
    CDS264..4481
    Translation

    Target ORF information:

    RefSeq Version XM_031904873.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031904873.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCTTCCTC TCCTCCCTCT CTTCCCCTCG CCACATCCTG CCCAAATTAC AGAAAAAGGC 
    AGAGCAGATT ATTTGGAAAC AGAATTCCCT GCTCAGACAG AAATAGAGGA CACAATGAAG
    GCACTGAGTC ATGATGTGAG CAGCCCCTGC AGAAAAACAC ACACAGTTAT TCAGAGCACA
    CCATTGGATC TCATAGATAC AGGAAAAAGT CTTAGGGTGC AGACAGAAAA ACCTCATCTT
    GTCAGCCTGG GAAGTGGACG ACTCAGCACT GCAATCACCC TACTACCACT GGAAGAAGGA
    AGGACAACCC TGGGTCATGC ATCTAAGGAC ATTCCCCTGC AGGGACCTGG CATTGCTGCA
    GAACATTGCT ACATTGAAAA CATCAGAGGA AACCTCACCC TATACCCATG TGGAAATCCT
    TGTGCCATCG ATGGACTGCT CATCACCCAA CCGACACGTC TCACTCAAGG GTGTATGGTC
    TGTCTGGGTC AGTCCACCTT CCTGAGATTT AACCACCCAG CTGAAGCTCA CTGGATGAAA
    ACCATGATTC CTTCCACTGG GAGAAGCCCT GCGCCAGGCT TGTACCCATC AGATTCTTCT
    TCCTCCTTGC TAAATGGTTC TCGTGATGCT GCATACCCGA GAACTGTACC TTCCCATACC
    TCACTAGTAA GCTCCATTGA AAAAAATCTT CAGGACATCA TGGACTCGTT GGTTTTAGAA
    GGGCCTGCAC CACTGAGCAG GAAGCTTTCC CAGACCTCAC TTAACCATAT CACCCCTATG
    ACCAATGGAG AGAACAGACG GTACTTGCTT TCATCTCCAA CAAGTCCACC TTACTCAAAC
    TCAGGTTATG ACAATACCTC TCCACTGTCT TCTCCTGGAA GTGGAAGTCT CACTAGCCCC
    TCTCCGGGTC AAGATGCAGG AACGCCAACA GCACCCCCTG TGGTGCCACT TCGAACTTCT
    AGCTTTAGCC ATTCCTTGCC ACGACCAGTT CCTACCTATG GAGGAAGCAA CCTTGACTTG
    CGTTCTAATC GCAACACAGG TGGTGGGTCT CTGCTTAGTC CTTCAAGCCC AGCAACAGGA
    AGCAGGATGG GGGGACTAAG TGTGCCAAGC AGTCCACTCC TAGGTAGTAA GTTTCATCAC
    CAGATCACAG ACCGCCCAAC CAGCCCTTTT AGACAGGTGG TAGGACCAAC ACGGAGTCTG
    CCACCAGAGA GTCCAAGACT TGGGCGGAGG AACTTTGAAA GCATGAGGGA CCTGCCTCCA
    CTTAGCCCTT CTCTGCCCCG AAGAAGTTCA TCTAGCTCCA TTAAACCTGC TCCAGACAGC
    CCATCTCGAG TTCCAGACAG CCCCAGCACT TTAGGGCGAC GTCGGGTGGG AAGTGCTAGC
    TCTCCTGGCA CAGAAAATAA TGCAAGTACA AGGCAGATAA GGGAGCGCAG CCCATCTCCC
    TCCTCTCTTC AAGAGACCTC TCGTCGACTA ACTCCAGCTT ATAGCCTGGG ATCGCTTGGG
    ACCCCTTCAC AAAGCCCCAG GGCACAAAGA AAAGGGCCTG TGCTACGGGA GCGCAAGAAC
    AGCATCAGTG AAATTTCTGG CAACCAAGAG GAGCTAATGG ACTACCACAG AAGGCAGAGA
    GAAGAAAGAC TTAGAGAGCA GGAAATGGAA AGGCTGGAGC GGCAGAGACT GGAAACCATT
    TTAAACTTGT GTGCCGAATA CAGTAAAACA GATGATACAG CTCCCCCAGC TCGCCTAGAT
    GAAATAAGGA GCTTCCCCAC TTTGGGGGGT ACTGAGGAAA CCACTGCATC CCTCTTCACT
    CGTAGACCTT CTTGGGAGAG CACCAGAAGT ACCAACCTGG AGGGAACAGG CAATAGGGTG
    AGAGCACAGG GAGAACACAA TGAGGAAGAG ACTCAGAGAG AAGAGAACAG CAGTACAGAG
    AGTACCCAAC ATGAGGAAGA TGCTGTAAGC CCAACTGGGG CTGCAGAATT ACTAGCCCTG
    GAGGAGCAAC GCAGGCACAC CCTGGGGCAT GTGGATCAGC TAAAGAATAG AGTGACTGAA
    TTGGAGGGTC AGTTGCAGGA AAGTGCAAGG GAGGCAGAGA TGGAACGTGC CCTTTTGCAA
    GGGGAACGGG AGGCTGAGCT GTCCCAGTTG CAGCATGAGC AAAGAATGGT CCAGCAGCTG
    CAGGACAGCC TGACCTCTTT AGAAAACAAC TTGCAGAAGG AGAAAGACAA GGCACGTGAG
    AAACTGGAGC AGGAGAGGAT GGAGCTGCAG AGTATTCAGC TTTCTCACCA CGACCTGCAG
    AGGCAGCTCC ATAACTGCCC TGAGGCTCTG CGGGAGCAGG TGCAGTGCCA GCTGACACGG
    GAGTCTGAAC TCCTGGAGAC GAAGATGAAG CTGTTTGAGG ATCTGGAGTT CCAGCAGCTA
    GAAAAGGAAA GCCGCCTGGA AGAAGAAAGA GAGACCACAA GTCAACAACT TTTACAGAGC
    AAGGGGGAAT GCCAGCGAAG CATTGCACGC AGGAAGGAAC GCCTGGCTGC TTTGGAGATG
    CAAGCCAATC AGATTCGCCT CCAGGCAGCA CAGGATGCAG ACCATCTTGG CCAGGAAAGA
    AGCGCCATAC TTTTACAGCT ACACAAGGAG AAAGAGAAGC TGGTTCAGTT GGAGAGAAGA
    TACCAGGCAC TAACAGGACG ACGGGGATTC TCAACTGGAT CAGCTGCTCT CCGAGAGTAC
    ATCACTTTAG ATCAGCTGCA GGATATTCTG GAAAGCATGC CCACAGGTTC TGCTCCAACT
    GCTGTGGCAA TGGCTGGCAC TGATATCAAC TCTGCCTCTT GTGCTCCTTC ACCTGACGAC
    CCTCCTCCTG TACATCTCTC GTCTTCATCT TCTTCCTCTC CCTCCATTTC TCTGGAGGTG
    AATTCCCCAA CAACAGAAGG TCACTCTGCC CCCCTAGCAC CTGATTTGGA TGCCTATTAC
    CAAGATTTAG TAACTGCACC TTCTGCCTCT TCTTCTCCAT CTTCTTCCCC TCCTCCACTC
    CCTGCTAAAT CCCACTCATC ACGTAAGGCT TTGCAGATTT CTCGCTCTCG GACAGAAATT
    GAGGGTGGAG GCACTCTTCC ACGGATGAAA ACAGTCTCCC CCGCTTCTTC TCACTTTTCA
    GTGGCCACTC TGGGCCGTAG TGTTTCCCCA AAGAATGCCC ATCTGACTCA GAATGGCACA
    GGCAGCCTCT CCAGGGGATT GGCAGTAACC ATGCAAGACA TTCAAAGCAA GAGACAGCTG
    GCACTGCAAC AAAAAGGTCA ACAAGTGATA GAGGAGCAGC GCAGACGTCT TGCAGAGTTG
    AAACAGAGAG CTGCAGTTGA GGCACAATCT CAGTGGGAAT CTCTCCATGG AGCCCCTAAT
    CAAGGGTACA CGCCTATAAT TCACCACTCC ATTCTACACC ATCAGCGGCC TCTCCATTCT
    CGTGAAGATG ACCCTACCCA TGGATTTGAC ACTCTCAGTC TGGAGAGTTC TGATAGTTTG
    GACACTAGTG TTTCTACAGG AAACTCTGCA TGTTCCCCTG ATAACATGTC AAGTGCTAGT
    GGGTTAGACA TGCTAAAGAT AGAGGAAATG GAAAGAATGC TTCTAGAAGC TCATGCAGAG
    CGTGCCAGGC TTGTAGAATC CAGGGAGCGT GAGGTGGAGG CACGCAGAAA AGCACTAGAA
    GATGAGAGGC GAAGGAGAGA GGAGCTGGAA AGGCGTCTTC AAGATGAGAC AGCTCACAGG
    CAAAGACTAG TAGAGAAGGA GGTCAAGATG AGGGAGAAGC ATTTTTCACA GGCTCGTCCT
    CTTACACGCT ATCTGCCAAT CCGGAAGGAA GACTTTGATC TCCGAGCCCA TGTTGAGTCC
    TCAGGCCATG CAGTAGATGC CTGCCCTCAC ATCATTTTAA GTGAGAAGAT GTGTCGTGGC
    TTCCTGACAA AAATGGGTGG AAAGATAAAA TCATGGAAGA AGCGCTGGTT TGTTTTTGAT
    CGCCTGAAAC GGACCCTGTC CTATTATGTA GATAAACATG AGGCAAAGCT GAAAGGAGTT
    ATATATTTCC AGGCCATAGA AGAGGTTTAT TATGACCACC TCAGGAGTGC AGCCAAGAGT
    CCTAACCCAG CTCTAACATT CTGTGTCAAG ACTCACGATC GCCTGTATTA CATGGTAGCT
    CCTTCCCCAG AGGCTATGCG GATATGGATG GATGTCATTG TAACTGGGGC AGAGGGTTAC
    ACTCAGTTCA TGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32420
    Clone ID Related Accession (Same CDS sequence) XM_012966183.2 , XM_012966183.3
    Accession Version XM_012966183.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3966bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pleckstrin homology-like domain family B member 1 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966183.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGATTTC TCGCTCTCGG ACAGAAATTG AGGGTGGAGG CACTCTTCCA
    CGGATGAAAA CAGTCTCCCC CGCTTCTTCT CACTTTTCAG TGGCCACTCT GGGCCGTAGT
    GTTTCCCCAA AGAATGCCCA TCTGACTCAG AATGGCACAG GCAGCCTCTC CAGGGGATTG
    GCAGTAACCA TGCAAGACAT TCAAAGCAAG AGACAGCTGG CACTGCAACA AAAAGGTCAA
    CAAGTGATAG AGGAGCAGCG CAGACGTCTT GCAGAGTTGA AACAGAGAGC TGCAGTTGAG
    GCACAATCTC AGTGGGAATC TCTCCATGGA GCCCCTAATC AAGGGTACAC GCCTATAATT
    CACCACTCCA TTCTACACCA TCAGCGGCCT CTCCATTCTC GTGAAGATGA CCCTACCCAT
    GGATTTGACA CTCTCAGTCT GGAGAGTTCT GATAGTTTGG ACACTAGTGT TTCTACAGGA
    AACTCTGCAT GTTCCCCTGA TAACATGTCA AGTGCTAGTG GGTTAGACAT GCTAAAGATA
    GAGGAAATGG AAAGAATGCT TCTAGAAGCT CATGCAGAGC GTGCCAGGCT TGTAGAATCC
    AGGGAGCGTG AGGTGGAGGC ACGCAGAAAA GCACTAGAAG ATGAGAGGCG AAGGAGAGAG
    GAGCTGGAAA GGCGTCTTCA AGATGAGACA GCTCACAGGC AAAGACTAGT AGAGAAGGAG
    GTCAAGATGA GGGAGAAGCA TTTTTCACAG GCTCGTCCTC TTACACGCTA TCTGCCAATC
    CGGAAGGAAG ACTTTGATCT CCGAGCCCAT GTTGAGTCCT CAGGCCATGC AGTAGATGCC
    TGCCCTCACA TCATTTTAAG TGAGAAGATG TGTCGTGGCT TCCTGACAAA AATGGGTGGA
    AAGATAAAAT CATGGAAGAA GCGCTGGTTT GTTTTTGATC GCCTGAAACG GACCCTGTCC
    TATTATGTAG ATAAACATGA GGCAAAGCTG AAAGGAGTTA TATATTTCCA GGCCATAGAA
    GAGGTTTATT ATGACCACCT CAGGAGTGCA GCCAAGAAAG GACTTTTTAA CCTCAGCCTG
    GCCACTCAGA TATCCGATTT TCTTTCTGTG CCCCTGTGGA AGGAGAGTCC TAACCCAGCT
    CTAACATTCT GTGTCAAGAC TCACGATCGC CTGTATTACA TGGTAGCTCC TTCCCCAGAG
    GCTATGCGGA TATGGATGGA TGTCATTGTA ACTGGGGCAG AGGGTTACAC TCAGTTCATG
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821637.1
    CDS58..4023
    Translation

    Target ORF information:

    RefSeq Version XM_012966183.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966183.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGATTTC TCGCTCTCGG ACAGAAATTG AGGGTGGAGG CACTCTTCCA
    CGGATGAAAA CAGTCTCCCC CGCTTCTTCT CACTTTTCAG TGGCCACTCT GGGCCGTAGT
    GTTTCCCCAA AGAATGCCCA TCTGACTCAG AATGGCACAG GCAGCCTCTC CAGGGGATTG
    GCAGTAACCA TGCAAGACAT TCAAAGCAAG AGACAGCTGG CACTGCAACA AAAAGGTCAA
    CAAGTGATAG AGGAGCAGCG CAGACGTCTT GCAGAGTTGA AACAGAGAGC TGCAGTTGAG
    GCACAATCTC AGTGGGAATC TCTCCATGGA GCCCCTAATC AAGGGTACAC GCCTATAATT
    CACCACTCCA TTCTACACCA TCAGCGGCCT CTCCATTCTC GTGAAGATGA CCCTACCCAT
    GGATTTGACA CTCTCAGTCT GGAGAGTTCT GATAGTTTGG ACACTAGTGT TTCTACAGGA
    AACTCTGCAT GTTCCCCTGA TAACATGTCA AGTGCTAGTG GGTTAGACAT GCTAAAGATA
    GAGGAAATGG AAAGAATGCT TCTAGAAGCT CATGCAGAGC GTGCCAGGCT TGTAGAATCC
    AGGGAGCGTG AGGTGGAGGC ACGCAGAAAA GCACTAGAAG ATGAGAGGCG AAGGAGAGAG
    GAGCTGGAAA GGCGTCTTCA AGATGAGACA GCTCACAGGC AAAGACTAGT AGAGAAGGAG
    GTCAAGATGA GGGAGAAGCA TTTTTCACAG GCTCGTCCTC TTACACGCTA TCTGCCAATC
    CGGAAGGAAG ACTTTGATCT CCGAGCCCAT GTTGAGTCCT CAGGCCATGC AGTAGATGCC
    TGCCCTCACA TCATTTTAAG TGAGAAGATG TGTCGTGGCT TCCTGACAAA AATGGGTGGA
    AAGATAAAAT CATGGAAGAA GCGCTGGTTT GTTTTTGATC GCCTGAAACG GACCCTGTCC
    TATTATGTAG ATAAACATGA GGCAAAGCTG AAAGGAGTTA TATATTTCCA GGCCATAGAA
    GAGGTTTATT ATGACCACCT CAGGAGTGCA GCCAAGAAAG GACTTTTTAA CCTCAGCCTG
    GCCACTCAGA TATCCGATTT TCTTTCTGTG CCCCTGTGGA AGGAGAGTCC TAACCCAGCT
    CTAACATTCT GTGTCAAGAC TCACGATCGC CTGTATTACA TGGTAGCTCC TTCCCCAGAG
    GCTATGCGGA TATGGATGGA TGTCATTGTA ACTGGGGCAG AGGGTTACAC TCAGTTCATG
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32421
    Clone ID Related Accession (Same CDS sequence) XM_012966184.2 , XM_012966184.3
    Accession Version XM_012966184.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3837bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pleckstrin homology-like domain family B member 1 isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966184.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG AGTCTGAACT CCTGGAGACG AAGATGAAGC TGTTTGAGGA TCTGGAGTTC
    CAGCAGCTAG AAAAGGAAAG CCGCCTGGAA GAAGAAAGAG AGACCACAAG TCAACAACTT
    TTACAGAGCA AGGGGGAATG CCAGCGAAGC ATTGCACGCA GGAAGGAACG CCTGGCTGCT
    TTGGAGATGC AAGCCAATCA GATTCGCCTC CAGGCAGCAC AGGATGCAGA CCATCTTGGC
    CAGGAAAGAA GCGCCATACT TTTACAGCTA CACAAGGAGA AAGAGAAGCT GGTTCAGTTG
    GAGAGAAGAT ACCAGGCACT AACAGGACGA CGGGGATTCT CAACTGGATC AGCTGCTCTC
    CGAGAGATTT CTCGCTCTCG GACAGAAATT GAGGGTGGAG GCACTCTTCC ACGGATGAAA
    ACAGTCTCCC CCGCTTCTTC TCACTTTTCA GTGGCCACTC TGGGCCGTAG TGTTTCCCCA
    AAGAATGCCC ATCTGACTCA GAATGGCACA GGCAGCCTCT CCAGGGGATT GGCAGTAACC
    ATGCAAGACA TTCAAAGCAA GAGACAGCTG GCACTGCAAC AAAAAGGTCA ACAAGTGATA
    GAGGAGCAGC GCAGACGTCT TGCAGAGTTG AAACAGAGAG CTGCAGTTGA GGCACAATCT
    CAGTGGGAAT CTCTCCATGG AGCCCCTAAT CAAGGGTACA CGCCTATAAT TCACCACTCC
    ATTCTACACC ATCAGCGGCC TCTCCATTCT CGTGAAGATG ACCCTACCCA TGGATTTGAC
    ACTCTCAGTC TGGAGAGTTC TGATAGTTTG GACACTAGTG TTTCTACAGG AAACTCTGCA
    TGTTCCCCTG ATAACATGTC AAGTGCTAGT GGGTTAGACA TGCTAAAGAT AGAGGAAATG
    GAAAGAATGC TTCTAGAAGC TCATGCAGAG CGTGCCAGGC TTGTAGAATC CAGGGAGCGT
    GAGGTGGAGG CACGCAGAAA AGCACTAGAA GATGAGAGGC GAAGGAGAGA GGAGCTGGAA
    AGGCGTCTTC AAGATGAGAC AGCTCACAGG CAAAGACTAG TAGAGAAGGA GGTCAAGATG
    AGGGAGAAGC ATTTTTCACA GGCTCGTCCT CTTACACGCT ATCTGCCAAT CCGGAAGGAA
    GACTTTGATC TCCGAGCCCA TGTTGAGTCC TCAGGCCATG CAGTAGATGC CTGCCCTCAC
    ATCATTTTAA GTGAGAAGAT GTGTCGTGGC TTCCTGACAA AAATGGGTGG AAAGATAAAA
    TCATGGAAGA AGCGCTGGTT TGTTTTTGAT CGCCTGAAAC GGACCCTGTC CTATTATGTA
    GATAAACATG AGGCAAAGCT GAAAGGAGTT ATATATTTCC AGGCCATAGA AGAGGTTTAT
    TATGACCACC TCAGGAGTGC AGCCAAGAAA GGACTTTTTA ACCTCAGCCT GGCCACTCAG
    ATATCCGATT TTCTTTCTGT GCCCCTGTGG AAGGAGAGTC CTAACCCAGC TCTAACATTC
    TGTGTCAAGA CTCACGATCG CCTGTATTAC ATGGTAGCTC CTTCCCCAGA GGCTATGCGG
    ATATGGATGG ATGTCATTGT AACTGGGGCA GAGGGTTACA CTCAGTTCAT GAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821638.1
    CDS58..3894
    Translation

    Target ORF information:

    RefSeq Version XM_012966184.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966184.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG AGTCTGAACT CCTGGAGACG AAGATGAAGC TGTTTGAGGA TCTGGAGTTC
    CAGCAGCTAG AAAAGGAAAG CCGCCTGGAA GAAGAAAGAG AGACCACAAG TCAACAACTT
    TTACAGAGCA AGGGGGAATG CCAGCGAAGC ATTGCACGCA GGAAGGAACG CCTGGCTGCT
    TTGGAGATGC AAGCCAATCA GATTCGCCTC CAGGCAGCAC AGGATGCAGA CCATCTTGGC
    CAGGAAAGAA GCGCCATACT TTTACAGCTA CACAAGGAGA AAGAGAAGCT GGTTCAGTTG
    GAGAGAAGAT ACCAGGCACT AACAGGACGA CGGGGATTCT CAACTGGATC AGCTGCTCTC
    CGAGAGATTT CTCGCTCTCG GACAGAAATT GAGGGTGGAG GCACTCTTCC ACGGATGAAA
    ACAGTCTCCC CCGCTTCTTC TCACTTTTCA GTGGCCACTC TGGGCCGTAG TGTTTCCCCA
    AAGAATGCCC ATCTGACTCA GAATGGCACA GGCAGCCTCT CCAGGGGATT GGCAGTAACC
    ATGCAAGACA TTCAAAGCAA GAGACAGCTG GCACTGCAAC AAAAAGGTCA ACAAGTGATA
    GAGGAGCAGC GCAGACGTCT TGCAGAGTTG AAACAGAGAG CTGCAGTTGA GGCACAATCT
    CAGTGGGAAT CTCTCCATGG AGCCCCTAAT CAAGGGTACA CGCCTATAAT TCACCACTCC
    ATTCTACACC ATCAGCGGCC TCTCCATTCT CGTGAAGATG ACCCTACCCA TGGATTTGAC
    ACTCTCAGTC TGGAGAGTTC TGATAGTTTG GACACTAGTG TTTCTACAGG AAACTCTGCA
    TGTTCCCCTG ATAACATGTC AAGTGCTAGT GGGTTAGACA TGCTAAAGAT AGAGGAAATG
    GAAAGAATGC TTCTAGAAGC TCATGCAGAG CGTGCCAGGC TTGTAGAATC CAGGGAGCGT
    GAGGTGGAGG CACGCAGAAA AGCACTAGAA GATGAGAGGC GAAGGAGAGA GGAGCTGGAA
    AGGCGTCTTC AAGATGAGAC AGCTCACAGG CAAAGACTAG TAGAGAAGGA GGTCAAGATG
    AGGGAGAAGC ATTTTTCACA GGCTCGTCCT CTTACACGCT ATCTGCCAAT CCGGAAGGAA
    GACTTTGATC TCCGAGCCCA TGTTGAGTCC TCAGGCCATG CAGTAGATGC CTGCCCTCAC
    ATCATTTTAA GTGAGAAGAT GTGTCGTGGC TTCCTGACAA AAATGGGTGG AAAGATAAAA
    TCATGGAAGA AGCGCTGGTT TGTTTTTGAT CGCCTGAAAC GGACCCTGTC CTATTATGTA
    GATAAACATG AGGCAAAGCT GAAAGGAGTT ATATATTTCC AGGCCATAGA AGAGGTTTAT
    TATGACCACC TCAGGAGTGC AGCCAAGAAA GGACTTTTTA ACCTCAGCCT GGCCACTCAG
    ATATCCGATT TTCTTTCTGT GCCCCTGTGG AAGGAGAGTC CTAACCCAGC TCTAACATTC
    TGTGTCAAGA CTCACGATCG CCTGTATTAC ATGGTAGCTC CTTCCCCAGA GGCTATGCGG
    ATATGGATGG ATGTCATTGT AACTGGGGCA GAGGGTTACA CTCAGTTCAT GAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32422
    Clone ID Related Accession (Same CDS sequence) XM_012966185.2 , XM_012966185.3
    Accession Version XM_012966185.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3768bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pleckstrin homology-like domain family B member 1 isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966185.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG AGTCTGAACT CCTGGAGACG AAGATGAAGC TGTTTGAGGA TCTGGAGTTC
    CAGCAGCTAG AAAAGGAAAG CCGCCTGGAA GAAGAAAGAG AGACCACAAG TCAACAACTT
    TTACAGAGCA AGGGGGAATG CCAGCGAAGC ATTGCACGCA GGAAGGAACG CCTGGCTGCT
    TTGGAGATGC AAGCCAATCA GATTCGCCTC CAGGCAGCAC AGGATGCAGA CCATCTTGGC
    CAGGAAAGAA GCGCCATACT TTTACAGCTA CACAAGGAGA AAGAGAAGCT GGTTCAGTTG
    GAGAGAAGAT ACCAGGCACT AACAGGACGA CGGGGATTCT CAACTGGATC AGCTGCTCTC
    CGAGAGATTT CTCGCTCTCG GACAGAAATT GAGGGTGGAG GCACTCTTCC ACGGATGAAA
    ACAGTCTCCC CCGCTTCTTC TCACTTTTCA GTGGCCACTC TGGGCCGTAG TGTTTCCCCA
    AAGAATGCCC ATCTGACTCA GAATGGCACA GGCAGCCTCT CCAGGGGATT GGCAGTAACC
    ATGCAAGACA TTCAAAGCAA GAGACAGCTG GCACTGCAAC AAAAAGGTCA ACAAGTGATA
    GAGGAGCAGC GCAGACGTCT TGCAGAGTTG AAACAGAGAG CTGCAGTTGA GGCACAATCT
    CAGTGGGAAT CTCTCCATGG AGCCCCTAAT CAAGGGTACA CGCCTATAAT TCACCACTCC
    ATTCTACACC ATCAGCGGCC TCTCCATTCT CGTGAAGATG ACCCTACCCA TGGATTTGAC
    ACTCTCAGTC TGGAGAGTTC TGATAGTTTG GACACTAGTG TTTCTACAGG AAACTCTGCA
    TGTTCCCCTG ATAACATGTC AAGTGCTAGT GGGTTAGACA TGCTAAAGAT AGAGGAAATG
    GAAAGAATGC TTCTAGAAGC TCATGCAGAG CGTGCCAGGC TTGTAGAATC CAGGGAGCGT
    GAGGTGGAGG CACGCAGAAA AGCACTAGAA GATGAGAGGC GAAGGAGAGA GGAGCTGGAA
    AGGCGTCTTC AAGATGAGAC AGCTCACAGG CAAAGACTAG TAGAGAAGGA GGTCAAGATG
    AGGGAGAAGC ATTTTTCACA GGCTCGTCCT CTTACACGCT ATCTGCCAAT CCGGAAGGAA
    GACTTTGATC TCCGAGCCCA TGTTGAGTCC TCAGGCCATG CAGTAGATGC CTGCCCTCAC
    ATCATTTTAA GTGAGAAGAT GTGTCGTGGC TTCCTGACAA AAATGGGTGG AAAGATAAAA
    TCATGGAAGA AGCGCTGGTT TGTTTTTGAT CGCCTGAAAC GGACCCTGTC CTATTATGTA
    GATAAACATG AGGCAAAGCT GAAAGGAGTT ATATATTTCC AGGCCATAGA AGAGGTTTAT
    TATGACCACC TCAGGAGTGC AGCCAAGAGT CCTAACCCAG CTCTAACATT CTGTGTCAAG
    ACTCACGATC GCCTGTATTA CATGGTAGCT CCTTCCCCAG AGGCTATGCG GATATGGATG
    GATGTCATTG TAACTGGGGC AGAGGGTTAC ACTCAGTTCA TGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821639.1
    CDS56..3823
    Translation

    Target ORF information:

    RefSeq Version XM_012966185.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966185.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG AGTCTGAACT CCTGGAGACG AAGATGAAGC TGTTTGAGGA TCTGGAGTTC
    CAGCAGCTAG AAAAGGAAAG CCGCCTGGAA GAAGAAAGAG AGACCACAAG TCAACAACTT
    TTACAGAGCA AGGGGGAATG CCAGCGAAGC ATTGCACGCA GGAAGGAACG CCTGGCTGCT
    TTGGAGATGC AAGCCAATCA GATTCGCCTC CAGGCAGCAC AGGATGCAGA CCATCTTGGC
    CAGGAAAGAA GCGCCATACT TTTACAGCTA CACAAGGAGA AAGAGAAGCT GGTTCAGTTG
    GAGAGAAGAT ACCAGGCACT AACAGGACGA CGGGGATTCT CAACTGGATC AGCTGCTCTC
    CGAGAGATTT CTCGCTCTCG GACAGAAATT GAGGGTGGAG GCACTCTTCC ACGGATGAAA
    ACAGTCTCCC CCGCTTCTTC TCACTTTTCA GTGGCCACTC TGGGCCGTAG TGTTTCCCCA
    AAGAATGCCC ATCTGACTCA GAATGGCACA GGCAGCCTCT CCAGGGGATT GGCAGTAACC
    ATGCAAGACA TTCAAAGCAA GAGACAGCTG GCACTGCAAC AAAAAGGTCA ACAAGTGATA
    GAGGAGCAGC GCAGACGTCT TGCAGAGTTG AAACAGAGAG CTGCAGTTGA GGCACAATCT
    CAGTGGGAAT CTCTCCATGG AGCCCCTAAT CAAGGGTACA CGCCTATAAT TCACCACTCC
    ATTCTACACC ATCAGCGGCC TCTCCATTCT CGTGAAGATG ACCCTACCCA TGGATTTGAC
    ACTCTCAGTC TGGAGAGTTC TGATAGTTTG GACACTAGTG TTTCTACAGG AAACTCTGCA
    TGTTCCCCTG ATAACATGTC AAGTGCTAGT GGGTTAGACA TGCTAAAGAT AGAGGAAATG
    GAAAGAATGC TTCTAGAAGC TCATGCAGAG CGTGCCAGGC TTGTAGAATC CAGGGAGCGT
    GAGGTGGAGG CACGCAGAAA AGCACTAGAA GATGAGAGGC GAAGGAGAGA GGAGCTGGAA
    AGGCGTCTTC AAGATGAGAC AGCTCACAGG CAAAGACTAG TAGAGAAGGA GGTCAAGATG
    AGGGAGAAGC ATTTTTCACA GGCTCGTCCT CTTACACGCT ATCTGCCAAT CCGGAAGGAA
    GACTTTGATC TCCGAGCCCA TGTTGAGTCC TCAGGCCATG CAGTAGATGC CTGCCCTCAC
    ATCATTTTAA GTGAGAAGAT GTGTCGTGGC TTCCTGACAA AAATGGGTGG AAAGATAAAA
    TCATGGAAGA AGCGCTGGTT TGTTTTTGAT CGCCTGAAAC GGACCCTGTC CTATTATGTA
    GATAAACATG AGGCAAAGCT GAAAGGAGTT ATATATTTCC AGGCCATAGA AGAGGTTTAT
    TATGACCACC TCAGGAGTGC AGCCAAGAGT CCTAACCCAG CTCTAACATT CTGTGTCAAG
    ACTCACGATC GCCTGTATTA CATGGTAGCT CCTTCCCCAG AGGCTATGCG GATATGGATG
    GATGTCATTG TAACTGGGGC AGAGGGTTAC ACTCAGTTCA TGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32404
    Clone ID Related Accession (Same CDS sequence) XM_012966165.3 , XM_012966165.2
    Accession Version XM_012966165.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4560bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pleckstrin homology-like domain family B member 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966165.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA
    GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG
    CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT
    CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA
    GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA
    ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT
    GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA
    ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA
    GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA
    TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA
    GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT
    CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC
    TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA
    CAAAAAGACA GTCTGAGCAC AGAAGATTCT CTTCCATCTG AAGTTGAAGG TCAACAAGTG
    ATAGAGGAGC AGCGCAGACG TCTTGCAGAG TTGAAACAGA GAGCTGCAGT TGAGGCACAA
    TCTCAGTGGG AATCTCTCCA TGGAGCCCCT AATCAAGGGT ACACGCCTAT AATTCACCAC
    TCCATTCTAC ACCATCAGCG GCCTCTCCAT TCTCGTGAAG ATGACCCTAC CCATGGATTT
    GACACTCTCA GTCTGGAGAG TTCTGATAGT TTGGACACTA GTGTTTCTAC AGGAAACTCT
    GCATGTTCCC CTGATAACAT GTCAAGTGCT AGTGGGTTAG ACATGCTAAA GATAGAGGAA
    ATGGAAAGAA TGCTTCTAGA AGCTCATGCA GAGCGTGCCA GGCTTGTAGA ATCCAGGGAG
    CGTGAGGTGG AGGCACGCAG AAAAGCACTA GAAGATGAGA GGCGAAGGAG AGAGGAGCTG
    GAAAGGCGTC TTCAAGATGA GACAGCTCAC AGGCAAAGAC TAGTAGAGAA GGAGGTCAAG
    ATGAGGGAGA AGCATTTTTC ACAGGCTCGT CCTCTTACAC GCTATCTGCC AATCCGGAAG
    GAAGACTTTG ATCTCCGAGC CCATGTTGAG TCCTCAGGCC ATGCAGTAGA TGCCTGCCCT
    CACATCATTT TAAGTGAGAA GATGTGTCGT GGCTTCCTGA CAAAAATGGG TGGAAAGATA
    AAATCATGGA AGAAGCGCTG GTTTGTTTTT GATCGCCTGA AACGGACCCT GTCCTATTAT
    GTAGATAAAC ATGAGGCAAA GCTGAAAGGA GTTATATATT TCCAGGCCAT AGAAGAGGTT
    TATTATGACC ACCTCAGGAG TGCAGCCAAG AAAGGACTTT TTAACCTCAG CCTGGCCACT
    CAGATATCCG ATTTTCTTTC TGTGCCCCTG TGGAAGGAGA GTCCTAACCC AGCTCTAACA
    TTCTGTGTCA AGACTCACGA TCGCCTGTAT TACATGGTAG CTCCTTCCCC AGAGGCTATG
    CGGATATGGA TGGATGTCAT TGTAACTGGG GCAGAGGGTT ACACTCAGTT CATGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821619.1
    CDS35..4594
    Translation

    Target ORF information:

    RefSeq Version XM_012966165.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966165.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA
    GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG
    CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT
    CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA
    GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA
    ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT
    GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA
    ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA
    GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA
    TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA
    GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT
    CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC
    TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA
    CAAAAAGACA GTCTGAGCAC AGAAGATTCT CTTCCATCTG AAGTTGAAGG TCAACAAGTG
    ATAGAGGAGC AGCGCAGACG TCTTGCAGAG TTGAAACAGA GAGCTGCAGT TGAGGCACAA
    TCTCAGTGGG AATCTCTCCA TGGAGCCCCT AATCAAGGGT ACACGCCTAT AATTCACCAC
    TCCATTCTAC ACCATCAGCG GCCTCTCCAT TCTCGTGAAG ATGACCCTAC CCATGGATTT
    GACACTCTCA GTCTGGAGAG TTCTGATAGT TTGGACACTA GTGTTTCTAC AGGAAACTCT
    GCATGTTCCC CTGATAACAT GTCAAGTGCT AGTGGGTTAG ACATGCTAAA GATAGAGGAA
    ATGGAAAGAA TGCTTCTAGA AGCTCATGCA GAGCGTGCCA GGCTTGTAGA ATCCAGGGAG
    CGTGAGGTGG AGGCACGCAG AAAAGCACTA GAAGATGAGA GGCGAAGGAG AGAGGAGCTG
    GAAAGGCGTC TTCAAGATGA GACAGCTCAC AGGCAAAGAC TAGTAGAGAA GGAGGTCAAG
    ATGAGGGAGA AGCATTTTTC ACAGGCTCGT CCTCTTACAC GCTATCTGCC AATCCGGAAG
    GAAGACTTTG ATCTCCGAGC CCATGTTGAG TCCTCAGGCC ATGCAGTAGA TGCCTGCCCT
    CACATCATTT TAAGTGAGAA GATGTGTCGT GGCTTCCTGA CAAAAATGGG TGGAAAGATA
    AAATCATGGA AGAAGCGCTG GTTTGTTTTT GATCGCCTGA AACGGACCCT GTCCTATTAT
    GTAGATAAAC ATGAGGCAAA GCTGAAAGGA GTTATATATT TCCAGGCCAT AGAAGAGGTT
    TATTATGACC ACCTCAGGAG TGCAGCCAAG AAAGGACTTT TTAACCTCAG CCTGGCCACT
    CAGATATCCG ATTTTCTTTC TGTGCCCCTG TGGAAGGAGA GTCCTAACCC AGCTCTAACA
    TTCTGTGTCA AGACTCACGA TCGCCTGTAT TACATGGTAG CTCCTTCCCC AGAGGCTATG
    CGGATATGGA TGGATGTCAT TGTAACTGGG GCAGAGGGTT ACACTCAGTT CATGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32405
    Clone ID Related Accession (Same CDS sequence) XM_012966167.2 , XM_012966167.3
    Accession Version XM_012966167.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4557bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pleckstrin homology-like domain family B member 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966167.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG GAAGATGCTG TAAGCCCAAC TGGGGCTGCA
    GAATTACTAG CCCTGGAGGA GCAACGCAGG CACACCCTGG GGCATGTGGA TCAGCTAAAG
    AATAGAGTGA CTGAATTGGA GGGTCAGTTG CAGGAAAGTG CAAGGGAGGC AGAGATGGAA
    CGTGCCCTTT TGCAAGGGGA ACGGGAGGCT GAGCTGTCCC AGTTGCAGCA TGAGCAAAGA
    ATGGTCCAGC AGCTGCAGGA CAGCCTGACC TCTTTAGAAA ACAACTTGCA GAAGGAGAAA
    GACAAGGCAC GTGAGAAACT GGAGCAGGAG AGGATGGAGC TGCAGAGTAT TCAGCTTTCT
    CACCACGACC TGCAGAGGCA GCTCCATAAC TGCCCTGAGG CTCTGCGGGA GCAGGTGCAG
    TGCCAGCTGA CACGGGAGTC TGAACTCCTG GAGACGAAGA TGAAGCTGTT TGAGGATCTG
    GAGTTCCAGC AGCTAGAAAA GGAAAGCCGC CTGGAAGAAG AAAGAGAGAC CACAAGTCAA
    CAACTTTTAC AGAGCAAGGG GGAATGCCAG CGAAGCATTG CACGCAGGAA GGAACGCCTG
    GCTGCTTTGG AGATGCAAGC CAATCAGATT CGCCTCCAGG CAGCACAGGA TGCAGACCAT
    CTTGGCCAGG AAAGAAGCGC CATACTTTTA CAGCTACACA AGGAGAAAGA GAAGCTGGTT
    CAGTTGGAGA GAAGATACCA GGCACTAACA GGACGACGGG GATTCTCAAC TGGATCAGCT
    GCTCTCCGAG AGGATGCTCT TCAAATCTAT GAGACTAGAG ACTGCTTACT CGCTCAAGCT
    GATGTTTCTT GCACACCTGC TATCCCTTCT GCCTGCTCTC ACTCCCCTTC CCGGCAGCAA
    GAGGAATACC TGTGCCTCTC TGATGTTCTC CGCTTGTACA GCAGCTCGCC TCTTGTTCCT
    GCTTTTCCCC TTCTCTCTGC TGCAGAGCCC ACCGCCGCCC TTGAGTACAT CACTTTAGAT
    CAGCTGCAGG ATATTCTGGA AAGCATGCCC ACAGGTTCTG CTCCAACTGC TGTGGCAATG
    GCTGGCACTG ATATCAACTC TGCCTCTTGT GCTCCTTCAC CTGACGACCC TCCTCCTGTA
    CATCTCTCGT CTTCATCTTC TTCCTCTCCC TCCATTTCTC TGGAGGTGAA TTCCCCAACA
    ACAGAAGGTC ACTCTGCCCC CCTAGCACCT GATTTGGATG CCTATTACCA AGATTTAGTA
    ACTGCACCTT CTGCCTCTTC TTCTCCATCT TCTTCCCCTC CTCCACTCCC TGCTAAATCC
    CACTCATCAC GTAAGGCTTT GCAGATTTCT CGCTCTCGGA CAGAAATTGA GGGTGGAGGC
    ACTCTTCCAC GGATGAAAAC AGTCTCCCCC GCTTCTTCTC ACTTTTCAGT GGCCACTCTG
    GGCCGTAGTG TTTCCCCAAA GAATGCCCAT CTGACTCAGA ATGGCACAGG CAGCCTCTCC
    AGGGGATTGG CAGTAACCAT GCAAGACATT CAAAGCAAGA GACAGCTGGC ACTGCAACAA
    AAAGACAGTC TGAGCACAGA AGATTCTCTT CCATCTGAAG TTGAAGGTCA ACAAGTGATA
    GAGGAGCAGC GCAGACGTCT TGCAGAGTTG AAACAGAGAG CTGCAGTTGA GGCACAATCT
    CAGTGGGAAT CTCTCCATGG AGCCCCTAAT CAAGGGTACA CGCCTATAAT TCACCACTCC
    ATTCTACACC ATCAGCGGCC TCTCCATTCT CGTGAAGATG ACCCTACCCA TGGATTTGAC
    ACTCTCAGTC TGGAGAGTTC TGATAGTTTG GACACTAGTG TTTCTACAGG AAACTCTGCA
    TGTTCCCCTG ATAACATGTC AAGTGCTAGT GGGTTAGACA TGCTAAAGAT AGAGGAAATG
    GAAAGAATGC TTCTAGAAGC TCATGCAGAG CGTGCCAGGC TTGTAGAATC CAGGGAGCGT
    GAGGTGGAGG CACGCAGAAA AGCACTAGAA GATGAGAGGC GAAGGAGAGA GGAGCTGGAA
    AGGCGTCTTC AAGATGAGAC AGCTCACAGG CAAAGACTAG TAGAGAAGGA GGTCAAGATG
    AGGGAGAAGC ATTTTTCACA GGCTCGTCCT CTTACACGCT ATCTGCCAAT CCGGAAGGAA
    GACTTTGATC TCCGAGCCCA TGTTGAGTCC TCAGGCCATG CAGTAGATGC CTGCCCTCAC
    ATCATTTTAA GTGAGAAGAT GTGTCGTGGC TTCCTGACAA AAATGGGTGG AAAGATAAAA
    TCATGGAAGA AGCGCTGGTT TGTTTTTGAT CGCCTGAAAC GGACCCTGTC CTATTATGTA
    GATAAACATG AGGCAAAGCT GAAAGGAGTT ATATATTTCC AGGCCATAGA AGAGGTTTAT
    TATGACCACC TCAGGAGTGC AGCCAAGAAA GGACTTTTTA ACCTCAGCCT GGCCACTCAG
    ATATCCGATT TTCTTTCTGT GCCCCTGTGG AAGGAGAGTC CTAACCCAGC TCTAACATTC
    TGTGTCAAGA CTCACGATCG CCTGTATTAC ATGGTAGCTC CTTCCCCAGA GGCTATGCGG
    ATATGGATGG ATGTCATTGT AACTGGGGCA GAGGGTTACA CTCAGTTCAT GAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821621.1
    CDS35..4591
    Translation

    Target ORF information:

    RefSeq Version XM_012966167.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966167.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG GAAGATGCTG TAAGCCCAAC TGGGGCTGCA
    GAATTACTAG CCCTGGAGGA GCAACGCAGG CACACCCTGG GGCATGTGGA TCAGCTAAAG
    AATAGAGTGA CTGAATTGGA GGGTCAGTTG CAGGAAAGTG CAAGGGAGGC AGAGATGGAA
    CGTGCCCTTT TGCAAGGGGA ACGGGAGGCT GAGCTGTCCC AGTTGCAGCA TGAGCAAAGA
    ATGGTCCAGC AGCTGCAGGA CAGCCTGACC TCTTTAGAAA ACAACTTGCA GAAGGAGAAA
    GACAAGGCAC GTGAGAAACT GGAGCAGGAG AGGATGGAGC TGCAGAGTAT TCAGCTTTCT
    CACCACGACC TGCAGAGGCA GCTCCATAAC TGCCCTGAGG CTCTGCGGGA GCAGGTGCAG
    TGCCAGCTGA CACGGGAGTC TGAACTCCTG GAGACGAAGA TGAAGCTGTT TGAGGATCTG
    GAGTTCCAGC AGCTAGAAAA GGAAAGCCGC CTGGAAGAAG AAAGAGAGAC CACAAGTCAA
    CAACTTTTAC AGAGCAAGGG GGAATGCCAG CGAAGCATTG CACGCAGGAA GGAACGCCTG
    GCTGCTTTGG AGATGCAAGC CAATCAGATT CGCCTCCAGG CAGCACAGGA TGCAGACCAT
    CTTGGCCAGG AAAGAAGCGC CATACTTTTA CAGCTACACA AGGAGAAAGA GAAGCTGGTT
    CAGTTGGAGA GAAGATACCA GGCACTAACA GGACGACGGG GATTCTCAAC TGGATCAGCT
    GCTCTCCGAG AGGATGCTCT TCAAATCTAT GAGACTAGAG ACTGCTTACT CGCTCAAGCT
    GATGTTTCTT GCACACCTGC TATCCCTTCT GCCTGCTCTC ACTCCCCTTC CCGGCAGCAA
    GAGGAATACC TGTGCCTCTC TGATGTTCTC CGCTTGTACA GCAGCTCGCC TCTTGTTCCT
    GCTTTTCCCC TTCTCTCTGC TGCAGAGCCC ACCGCCGCCC TTGAGTACAT CACTTTAGAT
    CAGCTGCAGG ATATTCTGGA AAGCATGCCC ACAGGTTCTG CTCCAACTGC TGTGGCAATG
    GCTGGCACTG ATATCAACTC TGCCTCTTGT GCTCCTTCAC CTGACGACCC TCCTCCTGTA
    CATCTCTCGT CTTCATCTTC TTCCTCTCCC TCCATTTCTC TGGAGGTGAA TTCCCCAACA
    ACAGAAGGTC ACTCTGCCCC CCTAGCACCT GATTTGGATG CCTATTACCA AGATTTAGTA
    ACTGCACCTT CTGCCTCTTC TTCTCCATCT TCTTCCCCTC CTCCACTCCC TGCTAAATCC
    CACTCATCAC GTAAGGCTTT GCAGATTTCT CGCTCTCGGA CAGAAATTGA GGGTGGAGGC
    ACTCTTCCAC GGATGAAAAC AGTCTCCCCC GCTTCTTCTC ACTTTTCAGT GGCCACTCTG
    GGCCGTAGTG TTTCCCCAAA GAATGCCCAT CTGACTCAGA ATGGCACAGG CAGCCTCTCC
    AGGGGATTGG CAGTAACCAT GCAAGACATT CAAAGCAAGA GACAGCTGGC ACTGCAACAA
    AAAGACAGTC TGAGCACAGA AGATTCTCTT CCATCTGAAG TTGAAGGTCA ACAAGTGATA
    GAGGAGCAGC GCAGACGTCT TGCAGAGTTG AAACAGAGAG CTGCAGTTGA GGCACAATCT
    CAGTGGGAAT CTCTCCATGG AGCCCCTAAT CAAGGGTACA CGCCTATAAT TCACCACTCC
    ATTCTACACC ATCAGCGGCC TCTCCATTCT CGTGAAGATG ACCCTACCCA TGGATTTGAC
    ACTCTCAGTC TGGAGAGTTC TGATAGTTTG GACACTAGTG TTTCTACAGG AAACTCTGCA
    TGTTCCCCTG ATAACATGTC AAGTGCTAGT GGGTTAGACA TGCTAAAGAT AGAGGAAATG
    GAAAGAATGC TTCTAGAAGC TCATGCAGAG CGTGCCAGGC TTGTAGAATC CAGGGAGCGT
    GAGGTGGAGG CACGCAGAAA AGCACTAGAA GATGAGAGGC GAAGGAGAGA GGAGCTGGAA
    AGGCGTCTTC AAGATGAGAC AGCTCACAGG CAAAGACTAG TAGAGAAGGA GGTCAAGATG
    AGGGAGAAGC ATTTTTCACA GGCTCGTCCT CTTACACGCT ATCTGCCAAT CCGGAAGGAA
    GACTTTGATC TCCGAGCCCA TGTTGAGTCC TCAGGCCATG CAGTAGATGC CTGCCCTCAC
    ATCATTTTAA GTGAGAAGAT GTGTCGTGGC TTCCTGACAA AAATGGGTGG AAAGATAAAA
    TCATGGAAGA AGCGCTGGTT TGTTTTTGAT CGCCTGAAAC GGACCCTGTC CTATTATGTA
    GATAAACATG AGGCAAAGCT GAAAGGAGTT ATATATTTCC AGGCCATAGA AGAGGTTTAT
    TATGACCACC TCAGGAGTGC AGCCAAGAAA GGACTTTTTA ACCTCAGCCT GGCCACTCAG
    ATATCCGATT TTCTTTCTGT GCCCCTGTGG AAGGAGAGTC CTAACCCAGC TCTAACATTC
    TGTGTCAAGA CTCACGATCG CCTGTATTAC ATGGTAGCTC CTTCCCCAGA GGCTATGCGG
    ATATGGATGG ATGTCATTGT AACTGGGGCA GAGGGTTACA CTCAGTTCAT GAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32406
    Clone ID Related Accession (Same CDS sequence) XM_012966168.2 , XM_012966168.3
    Accession Version XM_012966168.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4539bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pleckstrin homology-like domain family B member 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966168.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGAG
    TCTGAACTCC TGGAGACGAA GATGAAGCTG TTTGAGGATC TGGAGTTCCA GCAGCTAGAA
    AAGGAAAGCC GCCTGGAAGA AGAAAGAGAG ACCACAAGTC AACAACTTTT ACAGAGCAAG
    GGGGAATGCC AGCGAAGCAT TGCACGCAGG AAGGAACGCC TGGCTGCTTT GGAGATGCAA
    GCCAATCAGA TTCGCCTCCA GGCAGCACAG GATGCAGACC ATCTTGGCCA GGAAAGAAGC
    GCCATACTTT TACAGCTACA CAAGGAGAAA GAGAAGCTGG TTCAGTTGGA GAGAAGATAC
    CAGGCACTAA CAGGACGACG GGGATTCTCA ACTGGATCAG CTGCTCTCCG AGAGGATGCT
    CTTCAAATCT ATGAGACTAG AGACTGCTTA CTCGCTCAAG CTGATGTTTC TTGCACACCT
    GCTATCCCTT CTGCCTGCTC TCACTCCCCT TCCCGGCAGC AAGAGGAATA CCTGTGCCTC
    TCTGATGTTC TCCGCTTGTA CAGCAGCTCG CCTCTTGTTC CTGCTTTTCC CCTTCTCTCT
    GCTGCAGAGC CCACCGCCGC CCTTGAGTAC ATCACTTTAG ATCAGCTGCA GGATATTCTG
    GAAAGCATGC CCACAGGTTC TGCTCCAACT GCTGTGGCAA TGGCTGGCAC TGATATCAAC
    TCTGCCTCTT GTGCTCCTTC ACCTGACGAC CCTCCTCCTG TACATCTCTC GTCTTCATCT
    TCTTCCTCTC CCTCCATTTC TCTGGAGGTG AATTCCCCAA CAACAGAAGG TCACTCTGCC
    CCCCTAGCAC CTGATTTGGA TGCCTATTAC CAAGATTTAG TAACTGCACC TTCTGCCTCT
    TCTTCTCCAT CTTCTTCCCC TCCTCCACTC CCTGCTAAAT CCCACTCATC ACGTAAGGCT
    TTGCAGATTT CTCGCTCTCG GACAGAAATT GAGGGTGGAG GCACTCTTCC ACGGATGAAA
    ACAGTCTCCC CCGCTTCTTC TCACTTTTCA GTGGCCACTC TGGGCCGTAG TGTTTCCCCA
    AAGAATGCCC ATCTGACTCA GAATGGCACA GGCAGCCTCT CCAGGGGATT GGCAGTAACC
    ATGCAAGACA TTCAAAGCAA GAGACAGCTG GCACTGCAAC AAAAAGACAG TCTGAGCACA
    GAAGATTCTC TTCCATCTGA AGTTGAAGGT CAACAAGTGA TAGAGGAGCA GCGCAGACGT
    CTTGCAGAGT TGAAACAGAG AGCTGCAGTT GAGGCACAAT CTCAGTGGGA ATCTCTCCAT
    GGAGCCCCTA ATCAAGGGTA CACGCCTATA ATTCACCACT CCATTCTACA CCATCAGCGG
    CCTCTCCATT CTCGTGAAGA TGACCCTACC CATGGATTTG ACACTCTCAG TCTGGAGAGT
    TCTGATAGTT TGGACACTAG TGTTTCTACA GGAAACTCTG CATGTTCCCC TGATAACATG
    TCAAGTGCTA GTGGGTTAGA CATGCTAAAG ATAGAGGAAA TGGAAAGAAT GCTTCTAGAA
    GCTCATGCAG AGCGTGCCAG GCTTGTAGAA TCCAGGGAGC GTGAGGTGGA GGCACGCAGA
    AAAGCACTAG AAGATGAGAG GCGAAGGAGA GAGGAGCTGG AAAGGCGTCT TCAAGATGAG
    ACAGCTCACA GGCAAAGACT AGTAGAGAAG GAGGTCAAGA TGAGGGAGAA GCATTTTTCA
    CAGGCTCGTC CTCTTACACG CTATCTGCCA ATCCGGAAGG AAGACTTTGA TCTCCGAGCC
    CATGTTGAGT CCTCAGGCCA TGCAGTAGAT GCCTGCCCTC ACATCATTTT AAGTGAGAAG
    ATGTGTCGTG GCTTCCTGAC AAAAATGGGT GGAAAGATAA AATCATGGAA GAAGCGCTGG
    TTTGTTTTTG ATCGCCTGAA ACGGACCCTG TCCTATTATG TAGATAAACA TGAGGCAAAG
    CTGAAAGGAG TTATATATTT CCAGGCCATA GAAGAGGTTT ATTATGACCA CCTCAGGAGT
    GCAGCCAAGA AAGGACTTTT TAACCTCAGC CTGGCCACTC AGATATCCGA TTTTCTTTCT
    GTGCCCCTGT GGAAGGAGAG TCCTAACCCA GCTCTAACAT TCTGTGTCAA GACTCACGAT
    CGCCTGTATT ACATGGTAGC TCCTTCCCCA GAGGCTATGC GGATATGGAT GGATGTCATT
    GTAACTGGGG CAGAGGGTTA CACTCAGTTC ATGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821622.1
    CDS35..4573
    Translation

    Target ORF information:

    RefSeq Version XM_012966168.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966168.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGAG
    TCTGAACTCC TGGAGACGAA GATGAAGCTG TTTGAGGATC TGGAGTTCCA GCAGCTAGAA
    AAGGAAAGCC GCCTGGAAGA AGAAAGAGAG ACCACAAGTC AACAACTTTT ACAGAGCAAG
    GGGGAATGCC AGCGAAGCAT TGCACGCAGG AAGGAACGCC TGGCTGCTTT GGAGATGCAA
    GCCAATCAGA TTCGCCTCCA GGCAGCACAG GATGCAGACC ATCTTGGCCA GGAAAGAAGC
    GCCATACTTT TACAGCTACA CAAGGAGAAA GAGAAGCTGG TTCAGTTGGA GAGAAGATAC
    CAGGCACTAA CAGGACGACG GGGATTCTCA ACTGGATCAG CTGCTCTCCG AGAGGATGCT
    CTTCAAATCT ATGAGACTAG AGACTGCTTA CTCGCTCAAG CTGATGTTTC TTGCACACCT
    GCTATCCCTT CTGCCTGCTC TCACTCCCCT TCCCGGCAGC AAGAGGAATA CCTGTGCCTC
    TCTGATGTTC TCCGCTTGTA CAGCAGCTCG CCTCTTGTTC CTGCTTTTCC CCTTCTCTCT
    GCTGCAGAGC CCACCGCCGC CCTTGAGTAC ATCACTTTAG ATCAGCTGCA GGATATTCTG
    GAAAGCATGC CCACAGGTTC TGCTCCAACT GCTGTGGCAA TGGCTGGCAC TGATATCAAC
    TCTGCCTCTT GTGCTCCTTC ACCTGACGAC CCTCCTCCTG TACATCTCTC GTCTTCATCT
    TCTTCCTCTC CCTCCATTTC TCTGGAGGTG AATTCCCCAA CAACAGAAGG TCACTCTGCC
    CCCCTAGCAC CTGATTTGGA TGCCTATTAC CAAGATTTAG TAACTGCACC TTCTGCCTCT
    TCTTCTCCAT CTTCTTCCCC TCCTCCACTC CCTGCTAAAT CCCACTCATC ACGTAAGGCT
    TTGCAGATTT CTCGCTCTCG GACAGAAATT GAGGGTGGAG GCACTCTTCC ACGGATGAAA
    ACAGTCTCCC CCGCTTCTTC TCACTTTTCA GTGGCCACTC TGGGCCGTAG TGTTTCCCCA
    AAGAATGCCC ATCTGACTCA GAATGGCACA GGCAGCCTCT CCAGGGGATT GGCAGTAACC
    ATGCAAGACA TTCAAAGCAA GAGACAGCTG GCACTGCAAC AAAAAGACAG TCTGAGCACA
    GAAGATTCTC TTCCATCTGA AGTTGAAGGT CAACAAGTGA TAGAGGAGCA GCGCAGACGT
    CTTGCAGAGT TGAAACAGAG AGCTGCAGTT GAGGCACAAT CTCAGTGGGA ATCTCTCCAT
    GGAGCCCCTA ATCAAGGGTA CACGCCTATA ATTCACCACT CCATTCTACA CCATCAGCGG
    CCTCTCCATT CTCGTGAAGA TGACCCTACC CATGGATTTG ACACTCTCAG TCTGGAGAGT
    TCTGATAGTT TGGACACTAG TGTTTCTACA GGAAACTCTG CATGTTCCCC TGATAACATG
    TCAAGTGCTA GTGGGTTAGA CATGCTAAAG ATAGAGGAAA TGGAAAGAAT GCTTCTAGAA
    GCTCATGCAG AGCGTGCCAG GCTTGTAGAA TCCAGGGAGC GTGAGGTGGA GGCACGCAGA
    AAAGCACTAG AAGATGAGAG GCGAAGGAGA GAGGAGCTGG AAAGGCGTCT TCAAGATGAG
    ACAGCTCACA GGCAAAGACT AGTAGAGAAG GAGGTCAAGA TGAGGGAGAA GCATTTTTCA
    CAGGCTCGTC CTCTTACACG CTATCTGCCA ATCCGGAAGG AAGACTTTGA TCTCCGAGCC
    CATGTTGAGT CCTCAGGCCA TGCAGTAGAT GCCTGCCCTC ACATCATTTT AAGTGAGAAG
    ATGTGTCGTG GCTTCCTGAC AAAAATGGGT GGAAAGATAA AATCATGGAA GAAGCGCTGG
    TTTGTTTTTG ATCGCCTGAA ACGGACCCTG TCCTATTATG TAGATAAACA TGAGGCAAAG
    CTGAAAGGAG TTATATATTT CCAGGCCATA GAAGAGGTTT ATTATGACCA CCTCAGGAGT
    GCAGCCAAGA AAGGACTTTT TAACCTCAGC CTGGCCACTC AGATATCCGA TTTTCTTTCT
    GTGCCCCTGT GGAAGGAGAG TCCTAACCCA GCTCTAACAT TCTGTGTCAA GACTCACGAT
    CGCCTGTATT ACATGGTAGC TCCTTCCCCA GAGGCTATGC GGATATGGAT GGATGTCATT
    GTAACTGGGG CAGAGGGTTA CACTCAGTTC ATGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32407
    Clone ID Related Accession (Same CDS sequence) XM_012966169.3 , XM_012966169.2
    Accession Version XM_012966169.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4521bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pleckstrin homology-like domain family B member 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966169.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA
    GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG
    CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT
    CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA
    GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA
    ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT
    GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA
    ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA
    GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA
    TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA
    GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT
    CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC
    TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA
    CAAAAAGACA GTCTGAGCAC AGAAGATTCT CTTCCATCTG AAGTTGAAGG TCAACAAGTG
    ATAGAGGAGC AGCGCAGACG TCTTGCAGAG TTGAAACAGA GAGCTGCAGT TGAGGCACAA
    TCTCAGTGGG AATCTCTCCA TGGAGCCCCT AATCAAGGGT ACACGCCTAT AATTCACCAC
    TCCATTCTAC ACCATCAGCG GCCTCTCCAT TCTCGTGAAG ATGACCCTAC CCATGGATTT
    GACACTCTCA GTCTGGAGAG TTCTGATAGT TTGGACACTA GTGTTTCTAC AGGAAACTCT
    GCATGTTCCC CTGATAACAT GTCAAGTGCT AGTGGGTTAG ACATGCTAAA GATAGAGGAA
    ATGGAAAGAA TGCTTCTAGA AGCTCATGCA GAGCGTGCCA GGCTTGTAGA ATCCAGGGAG
    CGTGAGGTGG AGGCACGCAG AAAAGCACTA GAAGATGAGA GGCGAAGGAG AGAGGAGCTG
    GAAAGGCGTC TTCAAGATGA GACAGCTCAC AGGCAAAGAC TAGTAGAGAA GGAGGTCAAG
    ATGAGGGAGA AGCATTTTTC ACAGGCTCGT CCTCTTACAC GCTATCTGCC AATCCGGAAG
    GAAGACTTTG ATCTCCGAGC CCATGTTGAG TCCTCAGGCC ATGCAGTAGA TGCCTGCCCT
    CACATCATTT TAAGTGAGAA GATGTGTCGT GGCTTCCTGA CAAAAATGGG TGGAAAGATA
    AAATCATGGA AGAAGCGCTG GTTTGTTTTT GATCGCCTGA AACGGACCCT GTCCTATTAT
    GTAGATAAAC ATGAGGCAAA GCTGAAAGGA GTTATATATT TCCAGGCCAT AGAAGAGGTT
    TATTATGACC ACCTCAGGAG TGCAGCCAAG AAAGGACTTT TTAACCTCAG CCTGGCCACT
    AGTCCTAACC CAGCTCTAAC ATTCTGTGTC AAGACTCACG ATCGCCTGTA TTACATGGTA
    GCTCCTTCCC CAGAGGCTAT GCGGATATGG ATGGATGTCA TTGTAACTGG GGCAGAGGGT
    TACACTCAGT TCATGAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821623.1
    CDS35..4555
    Translation

    Target ORF information:

    RefSeq Version XM_012966169.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966169.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA
    GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG
    CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT
    CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA
    GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA
    ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT
    GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA
    ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA
    GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA
    TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA
    GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT
    CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC
    TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA
    CAAAAAGACA GTCTGAGCAC AGAAGATTCT CTTCCATCTG AAGTTGAAGG TCAACAAGTG
    ATAGAGGAGC AGCGCAGACG TCTTGCAGAG TTGAAACAGA GAGCTGCAGT TGAGGCACAA
    TCTCAGTGGG AATCTCTCCA TGGAGCCCCT AATCAAGGGT ACACGCCTAT AATTCACCAC
    TCCATTCTAC ACCATCAGCG GCCTCTCCAT TCTCGTGAAG ATGACCCTAC CCATGGATTT
    GACACTCTCA GTCTGGAGAG TTCTGATAGT TTGGACACTA GTGTTTCTAC AGGAAACTCT
    GCATGTTCCC CTGATAACAT GTCAAGTGCT AGTGGGTTAG ACATGCTAAA GATAGAGGAA
    ATGGAAAGAA TGCTTCTAGA AGCTCATGCA GAGCGTGCCA GGCTTGTAGA ATCCAGGGAG
    CGTGAGGTGG AGGCACGCAG AAAAGCACTA GAAGATGAGA GGCGAAGGAG AGAGGAGCTG
    GAAAGGCGTC TTCAAGATGA GACAGCTCAC AGGCAAAGAC TAGTAGAGAA GGAGGTCAAG
    ATGAGGGAGA AGCATTTTTC ACAGGCTCGT CCTCTTACAC GCTATCTGCC AATCCGGAAG
    GAAGACTTTG ATCTCCGAGC CCATGTTGAG TCCTCAGGCC ATGCAGTAGA TGCCTGCCCT
    CACATCATTT TAAGTGAGAA GATGTGTCGT GGCTTCCTGA CAAAAATGGG TGGAAAGATA
    AAATCATGGA AGAAGCGCTG GTTTGTTTTT GATCGCCTGA AACGGACCCT GTCCTATTAT
    GTAGATAAAC ATGAGGCAAA GCTGAAAGGA GTTATATATT TCCAGGCCAT AGAAGAGGTT
    TATTATGACC ACCTCAGGAG TGCAGCCAAG AAAGGACTTT TTAACCTCAG CCTGGCCACT
    AGTCCTAACC CAGCTCTAAC ATTCTGTGTC AAGACTCACG ATCGCCTGTA TTACATGGTA
    GCTCCTTCCC CAGAGGCTAT GCGGATATGG ATGGATGTCA TTGTAACTGG GGCAGAGGGT
    TACACTCAGT TCATGAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32408
    Clone ID Related Accession (Same CDS sequence) XM_012966170.3 , XM_012966170.2
    Accession Version XM_012966170.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4518bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pleckstrin homology-like domain family B member 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966170.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA
    GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG
    CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT
    CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA
    GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA
    ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT
    GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA
    ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA
    GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA
    TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA
    GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT
    CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC
    TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA
    CAAAAAGGTC AACAAGTGAT AGAGGAGCAG CGCAGACGTC TTGCAGAGTT GAAACAGAGA
    GCTGCAGTTG AGGCACAATC TCAGTGGGAA TCTCTCCATG GAGCCCCTAA TCAAGGGTAC
    ACGCCTATAA TTCACCACTC CATTCTACAC CATCAGCGGC CTCTCCATTC TCGTGAAGAT
    GACCCTACCC ATGGATTTGA CACTCTCAGT CTGGAGAGTT CTGATAGTTT GGACACTAGT
    GTTTCTACAG GAAACTCTGC ATGTTCCCCT GATAACATGT CAAGTGCTAG TGGGTTAGAC
    ATGCTAAAGA TAGAGGAAAT GGAAAGAATG CTTCTAGAAG CTCATGCAGA GCGTGCCAGG
    CTTGTAGAAT CCAGGGAGCG TGAGGTGGAG GCACGCAGAA AAGCACTAGA AGATGAGAGG
    CGAAGGAGAG AGGAGCTGGA AAGGCGTCTT CAAGATGAGA CAGCTCACAG GCAAAGACTA
    GTAGAGAAGG AGGTCAAGAT GAGGGAGAAG CATTTTTCAC AGGCTCGTCC TCTTACACGC
    TATCTGCCAA TCCGGAAGGA AGACTTTGAT CTCCGAGCCC ATGTTGAGTC CTCAGGCCAT
    GCAGTAGATG CCTGCCCTCA CATCATTTTA AGTGAGAAGA TGTGTCGTGG CTTCCTGACA
    AAAATGGGTG GAAAGATAAA ATCATGGAAG AAGCGCTGGT TTGTTTTTGA TCGCCTGAAA
    CGGACCCTGT CCTATTATGT AGATAAACAT GAGGCAAAGC TGAAAGGAGT TATATATTTC
    CAGGCCATAG AAGAGGTTTA TTATGACCAC CTCAGGAGTG CAGCCAAGAA AGGACTTTTT
    AACCTCAGCC TGGCCACTCA GATATCCGAT TTTCTTTCTG TGCCCCTGTG GAAGGAGAGT
    CCTAACCCAG CTCTAACATT CTGTGTCAAG ACTCACGATC GCCTGTATTA CATGGTAGCT
    CCTTCCCCAG AGGCTATGCG GATATGGATG GATGTCATTG TAACTGGGGC AGAGGGTTAC
    ACTCAGTTCA TGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821624.1
    CDS35..4552
    Translation

    Target ORF information:

    RefSeq Version XM_012966170.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966170.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA
    GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG
    CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT
    CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA
    GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA
    ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT
    GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA
    ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA
    GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA
    TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA
    GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT
    CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC
    TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA
    CAAAAAGGTC AACAAGTGAT AGAGGAGCAG CGCAGACGTC TTGCAGAGTT GAAACAGAGA
    GCTGCAGTTG AGGCACAATC TCAGTGGGAA TCTCTCCATG GAGCCCCTAA TCAAGGGTAC
    ACGCCTATAA TTCACCACTC CATTCTACAC CATCAGCGGC CTCTCCATTC TCGTGAAGAT
    GACCCTACCC ATGGATTTGA CACTCTCAGT CTGGAGAGTT CTGATAGTTT GGACACTAGT
    GTTTCTACAG GAAACTCTGC ATGTTCCCCT GATAACATGT CAAGTGCTAG TGGGTTAGAC
    ATGCTAAAGA TAGAGGAAAT GGAAAGAATG CTTCTAGAAG CTCATGCAGA GCGTGCCAGG
    CTTGTAGAAT CCAGGGAGCG TGAGGTGGAG GCACGCAGAA AAGCACTAGA AGATGAGAGG
    CGAAGGAGAG AGGAGCTGGA AAGGCGTCTT CAAGATGAGA CAGCTCACAG GCAAAGACTA
    GTAGAGAAGG AGGTCAAGAT GAGGGAGAAG CATTTTTCAC AGGCTCGTCC TCTTACACGC
    TATCTGCCAA TCCGGAAGGA AGACTTTGAT CTCCGAGCCC ATGTTGAGTC CTCAGGCCAT
    GCAGTAGATG CCTGCCCTCA CATCATTTTA AGTGAGAAGA TGTGTCGTGG CTTCCTGACA
    AAAATGGGTG GAAAGATAAA ATCATGGAAG AAGCGCTGGT TTGTTTTTGA TCGCCTGAAA
    CGGACCCTGT CCTATTATGT AGATAAACAT GAGGCAAAGC TGAAAGGAGT TATATATTTC
    CAGGCCATAG AAGAGGTTTA TTATGACCAC CTCAGGAGTG CAGCCAAGAA AGGACTTTTT
    AACCTCAGCC TGGCCACTCA GATATCCGAT TTTCTTTCTG TGCCCCTGTG GAAGGAGAGT
    CCTAACCCAG CTCTAACATT CTGTGTCAAG ACTCACGATC GCCTGTATTA CATGGTAGCT
    CCTTCCCCAG AGGCTATGCG GATATGGATG GATGTCATTG TAACTGGGGC AGAGGGTTAC
    ACTCAGTTCA TGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa61272
    Clone ID Related Accession (Same CDS sequence) XM_031904869.1
    Accession Version XM_031904869.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4557bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pleckstrin homology-like domain family B member 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA
    GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG
    CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT
    CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA
    GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA
    ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT
    GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA
    ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA
    GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA
    TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA
    GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT
    CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC
    TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA
    CAAAAAGACA GTCTGAGCAC AGAAGATTCT CTTCCATCTG AAGTTGAAGG TCAACAAGTG
    ATAGAGGAGC AGCGCAGACG TCTTGCAGAG TTGAAACAGA GAGCTGCAGT TGAGGCACAA
    TCTCAGTGGG AATCTCTCCA TGGAGCCCCT AATCAAGGGT ACACGCCTAT AATTCACCAC
    TCCATTCTAC ACCATCAGCG GCCTCTCCAT TCTCGTGAAG ATGACCCTAC CCATGGATTT
    GACACTCTCA GTCTGGAGAG TTCTGATAGT TTGGACACTA GTGTTTCTAC AGGAAACTCT
    GCATGTTCCC CTGATAACAT GTCAAGTGCT AGTGGGTTAG ACATGCTAAA GATAGAGGAA
    ATGGAAAGAA TGCTTCTAGA AGCTCATGCA GAGCGTGCCA GGCTTGTAGA ATCCAGGGAG
    CGTGAGGTGG AGGCACGCAG AAAAGCACTA GAAGATGAGA GGCGAAGGAG AGAGGAGCTG
    GAAAGGCGTC TTCAAGATGA GACAGCTCAC AGGCAAAGAC TAGTAGAGAA GGAGGTCAAG
    ATGAGGGAGA AGCATTTTTC ACAGGCTCGT CCTCTTACAC GCTATCTGCC AATCCGGAAG
    GAAGACTTTG ATCTCCGAGC CCATGTTGAG TCCTCAGGCC ATGCAGTAGA TGCCTGCCCT
    CACATCATTT TAAGTGAGAA GATGTGTCGT GGCTTCCTGA CAAAAATGGG TGGAAAGATA
    AAATCATGGA AGAAGCGCTG GTTTGTTTTT GATCGCCTGA AACGGACCCT GTCCTATTAT
    GTAGATAAAC ATGAGGCAAA GCTGAAAGGA GTTATATATT TCCAGGCCAT AGAAGAGGTT
    TATTATGACC ACCTCAGGAG TGCAGCCAAG AAAGGACTTT TTAACCTCAG CCTGGCCACT
    ATATCCGATT TTCTTTCTGT GCCCCTGTGG AAGGAGAGTC CTAACCCAGC TCTAACATTC
    TGTGTCAAGA CTCACGATCG CCTGTATTAC ATGGTAGCTC CTTCCCCAGA GGCTATGCGG
    ATATGGATGG ATGTCATTGT AACTGGGGCA GAGGGTTACA CTCAGTTCAT GAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031760729.1
    CDS35..4591
    Translation

    Target ORF information:

    RefSeq Version XM_031904869.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031904869.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA
    GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG
    CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT
    CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA
    GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA
    ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT
    GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA
    ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA
    GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA
    TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA
    GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT
    CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC
    TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA
    CAAAAAGACA GTCTGAGCAC AGAAGATTCT CTTCCATCTG AAGTTGAAGG TCAACAAGTG
    ATAGAGGAGC AGCGCAGACG TCTTGCAGAG TTGAAACAGA GAGCTGCAGT TGAGGCACAA
    TCTCAGTGGG AATCTCTCCA TGGAGCCCCT AATCAAGGGT ACACGCCTAT AATTCACCAC
    TCCATTCTAC ACCATCAGCG GCCTCTCCAT TCTCGTGAAG ATGACCCTAC CCATGGATTT
    GACACTCTCA GTCTGGAGAG TTCTGATAGT TTGGACACTA GTGTTTCTAC AGGAAACTCT
    GCATGTTCCC CTGATAACAT GTCAAGTGCT AGTGGGTTAG ACATGCTAAA GATAGAGGAA
    ATGGAAAGAA TGCTTCTAGA AGCTCATGCA GAGCGTGCCA GGCTTGTAGA ATCCAGGGAG
    CGTGAGGTGG AGGCACGCAG AAAAGCACTA GAAGATGAGA GGCGAAGGAG AGAGGAGCTG
    GAAAGGCGTC TTCAAGATGA GACAGCTCAC AGGCAAAGAC TAGTAGAGAA GGAGGTCAAG
    ATGAGGGAGA AGCATTTTTC ACAGGCTCGT CCTCTTACAC GCTATCTGCC AATCCGGAAG
    GAAGACTTTG ATCTCCGAGC CCATGTTGAG TCCTCAGGCC ATGCAGTAGA TGCCTGCCCT
    CACATCATTT TAAGTGAGAA GATGTGTCGT GGCTTCCTGA CAAAAATGGG TGGAAAGATA
    AAATCATGGA AGAAGCGCTG GTTTGTTTTT GATCGCCTGA AACGGACCCT GTCCTATTAT
    GTAGATAAAC ATGAGGCAAA GCTGAAAGGA GTTATATATT TCCAGGCCAT AGAAGAGGTT
    TATTATGACC ACCTCAGGAG TGCAGCCAAG AAAGGACTTT TTAACCTCAG CCTGGCCACT
    ATATCCGATT TTCTTTCTGT GCCCCTGTGG AAGGAGAGTC CTAACCCAGC TCTAACATTC
    TGTGTCAAGA CTCACGATCG CCTGTATTAC ATGGTAGCTC CTTCCCCAGA GGCTATGCGG
    ATATGGATGG ATGTCATTGT AACTGGGGCA GAGGGTTACA CTCAGTTCAT GAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa02934
    Clone ID Related Accession (Same CDS sequence) NM_001130332.1
    Accession Version NM_001130332.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4104bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pleckstrin homology-like domain family B member 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC167599.1. ##Evidence-Data-START## Transcript exon combination :: BC167599.1 [ECO:0000332] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAAGGCAC TGAGTCATGA TGTGAGCAGC CCCTGCAGAA AAACACACAC AGTTATTCAG 
    AGCACACCAT TGGATCTCAT AGATACAGGA AAAAGTCTTA GGGTGCAGAC AGAAAAACCT
    CATCTTGTCA GCCTGGGAAG TGGACGACTC AGCACTGCAA TCACCCTACT ACCACTGGAA
    GAAGGAAGGA CAACCCTGGG TCATGCATCT AAGGACATTC CCCTGCAGGG ACCTGGCATT
    GCTGCAGAAC ATTGCTACAT TGAAAACATC AGAGGAAACC TCACCCTATA CCCATGTGGA
    AATCCTTGTG CCATCGATGG ACTGCTCATC ACCCAACCGA CACGTCTCAC TCAAGGGTGT
    ATGGTCTGTC TGGGTCAGTC CACCTTCCTG AGATTTAACC ACCCAGCTGA AGCTCACTGG
    ATGAAAACCA TGATTCCTTC CACTGGGAGA AGCCCTGCGC CAGGCTTGTA CCCATCAGAT
    TCTTCTTCCT CCTTGCTAAA TGGTTCTCGT GATGCTGCAT ACCCGAGAAC TGTACCTTCC
    CATACCTCAC TAGTAAGCTC CATTGAAAAA AATCTTCAGG ACATCATGGA CTCGTTGGTT
    TTAGAAGGGC CTGCACCACT GAGCAGGAAG CTTTCCCAGA CCTCACTTAA CCATATCACC
    CCTATGACCA ATGGAGAGAA CAGACGGTAC TTGCTTTCAT CTCCAACAAG TCCACCTTAC
    TCAAACTCAG GTTATGACAA TACCTCTCCA CTGTCTTCTC CTGGAAGTGG AAGTCTCACT
    AGCCCCTCTC CGGGTCAAGA TGCAGGAACG CCAACAGCAC CCCCTGTGGT GCCACTTCGA
    ACTTCTAGCT TTAGCCATTC CTTGCCACGA CCAGTTCCTA CCTATGGAGG AAGCAACCTT
    GACTTGCGTT CTAATCGCAA CACAGGTGGT GGGTCTCTGC TTAGTCCTTC AAGCCCAGCA
    ACAGGAAGCA GGATGGGGGG ACTAAGTGTG CCAAGCAGTC CACTCCTAGG TAGTAAGTTT
    CATCACCAGA TCACAGACCG CCCAACCAGC CCTTTTAGAC AGGTGGTAGG ACCAACACGG
    AGTCTGCCAC CAGAGAGTCC AAGACTTGGG CGGAGGAACT TTGAAAGCAT GAGGGACCTG
    CCTCCACTTA GCCCTTCTCT GCCCCGAAGA AGTTCATCTA GCTCCATTAA ACCTGCTCCA
    GACAGCCCAT CTCGAGTTCC AGACAGCCCC AGCACTTTAG GGCGACGTCG GGTGGGAAGT
    GCTAGCTCTC CTGGCACAGA AAATAATGCA AGTACAAGGC AGATAAGGGA GCGCAGCCCA
    TCTCCCTCCT CTCTTCAAGA GACCTCTCGT CGACTAACTC CAGCTTATAG CCTGGGATCG
    CTTGGGACCC CTTCACAAAG CCCCAGGGCA CAAAGAAAAG GGCCTGTGCT ACGGGAGCGC
    AAGAACAGCA TCAGTGAAAT TTCTGGCAAC CAAGAGGAGC TAATGGACTA CCACAGAAGG
    CAGAGAGAAG AAAGACTTAG AGAGCAGGAA ATGGAAAGGC TGGAGCGGCA GAGACTGGAA
    ACCATTTTAA ACTTGTGTGC CGAATACAGT AAAACAGATG ATACAGCTCC CCCAGCTCGC
    CTAGATGAAA TAAGGAGCTT CCCCACTTTG GGGGGTACTG AGGAAACCAC TGCATCCCTC
    TTCACTCGTA GACCTTCTTG GGAGAGCACC AGAAGTACCA ACCTGGAGGG AACAGGCAAT
    AGGGTGAGAG CACAGGGAGA ACACAATGAG GAAGAGACTC AGAGAGAAGA GAACAGCAGT
    ACAGAGAGTA CCCAACATGA GGAAGATGCT GTAAGCCCAA CTGGGGCTGC AGAATTACTA
    GCCCTGGAGG AGCAACGCAG GCACACCCTG GGGCATGTGG ATCAGCTAAA GAATAGAGTG
    ACTGAATTGG AGGGTCAGTT GCAGGAAAGT GCAAGGGAGG CAGAGATGGA ACGTGCCCTT
    TTGCAAGGGG AACGGGAGGC TGAGCTGTCC CAGTTGCAGC ATGAGCAAAG AATGGTCCAG
    CAGCTGCAGG ACAGCCTGAC CTCTTTAGAA AACAACTTGC AGAAGGAGAA AGACAAGGCA
    CGTGAGAAAC TGGAGCAGGA GAGGATGGAG CTGCAGAGTA TTCAGCTTTC TCACCACGAC
    CTGCAGAGGC AGCTCCATAA CTGCCCTGAG GCTCTGCGGG AGCAGGTGCA GTGCCAGCTG
    ACACGGGAGT CTGAACTCCT GGAGACGAAG ATGAAGCTGT TTGAGGATCT GGAGTTCCAG
    CAGCTAGAAA AGGAAAGCCG CCTGGAAGAA GAAAGAGAGA CCACAAGTCA ACAACTTTTA
    CAGAGCAAGG GGGAATGCCA GCGAAGCATT GCACGCAGGA AGGAACGCCT GGCTGCTTTG
    GAGATGCAAG CCAATCAGAT TCGCCTCCAG GCAGCACAGG ATGCAGACCA TCTTGGCCAG
    GAAAGAAGCG CCATACTTTT ACAGCTACAC AAGGAGAAAG AGAAGCTGGT TCAGTTGGAG
    AGAAGATACC AGGCACTAAC AGGACGACGG GGATTCTCAA CTGGATCAGC TGCTCTCCGA
    GAGTACATCA CTTTAGATCA GCTGCAGGAT ATTCTGGAAA GCATGCCCAC AGGTTCTGCT
    CCAACTGCTG TGGCAATGGC TGGCACTGAT ATCAACTCTG CCTCTTGTGC TCCTTCACCT
    GACGACCCTC CTCCTGTACA TCTCTCGTCT TCATCTTCTT CCTCTCCCTC CATTTCTCTG
    GAGGTGAATT CCCCAACAAC AGAAGGTCAC TCTGCCCCCC TAGCACCTGA TTTGGATGCC
    TATTACCAAG ATTTAGTAAC TGCACCTTCT GCCTCTTCTT CTCCATCTTC TTCCCCTCCT
    CCACTCCCTG CTAAATCCCA CTCATCACGT AAGGCTTTGC AGATTTCTCG CTCTCGGACA
    GAAATTGAGG GTGGAGGCAC TCTTCCACGG ATGAAAACAG TCTCCCCCGC TTCTTCTCAC
    TTTTCAGTGG CCACTCTGGG CCGTAGTGTT TCCCCAAAGA ATGCCCATCT GACTCAGAAT
    GGCACAGGCA GCCTCTCCAG GGGATTGGCA GTAACCATGC AAGACATTCA AAGCAAGAGA
    CAGCTGGCAC TGCAACAAAA AGGTCAACAA GTGATAGAGG AGCAGCGCAG ACGTCTTGCA
    GAGTTGAAAC AGAGAGCTGC AGTTGAGGCA CAATCTCAGT GGGAATCTCT CCATGGAGCC
    CCTAATCAAG GGTACACGCC TATAATTCAC CACTCCATTC TACACCATCA GCGGCCTCTC
    CATTCTCGTG AAGATGACCC TACCCATGGA TTTGACACTC TCAGTCTGGA GAGTTCTGAT
    AGTTTGGACA CTAGTGTTTC TACAGGAAAC TCTGCATGTT CCCCTGATAA CATGTCAAGT
    GCTAGTGGGT TAGACATGCT AAAGATAGAG GAAATGGAAA GAATGCTTCT AGAAGCTCAT
    GCAGAGCGTG CCAGGCTTGT AGAATCCAGG GAGCGTGAGG TGGAGGCACG CAGAAAAGCA
    CTAGAAGATG AGAGGCGAAG GAGAGAGGAG CTGGAAAGGC GTCTTCAAGA TGAGACAGCT
    CACAGGCAAA GACTAGTAGA GAAGGAGGTC AAGATGAGGG AGAAGCATTT TTCACAGGCT
    CGTCCTCTTA CACGCTATCT GCCAATCCGG AAGGAAGACT TTGATCTCCG AGCCCATGTT
    GAGTCCTCAG GCCATGCAGT AGATGCCTGC CCTCACATCA TTTTAAGTGA GAAGATGTGT
    CGTGGCTTCC TGACAAAAAT GGGTGGAAAG ATAAAATCAT GGAAGAAGCG CTGGTTTGTT
    TTTGATCGCC TGAAACGGAC CCTGTCCTAT TATGTAGATA AACATGAGGC AAAGCTGAAA
    GGAGTTATAT ATTTCCAGGC CATAGAAGAG GTTTATTATG ACCACCTCAG GAGTGCAGCC
    AAGAGTCCTA ACCCAGCTCT AACATTCTGT GTCAAGACTC ACGATCGCCT GTATTACATG
    GTAGCTCCTT CCCCAGAGGC TATGCGGATA TGGATGGATG TCATTGTAAC TGGGGCAGAG
    GGTTACACTC AGTTCATGAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001123804.1
    CDS378..4481
    Translation

    Target ORF information:

    RefSeq Version NM_001130332.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001130332.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAAGGCAC TGAGTCATGA TGTGAGCAGC CCCTGCAGAA AAACACACAC AGTTATTCAG 
    AGCACACCAT TGGATCTCAT AGATACAGGA AAAAGTCTTA GGGTGCAGAC AGAAAAACCT
    CATCTTGTCA GCCTGGGAAG TGGACGACTC AGCACTGCAA TCACCCTACT ACCACTGGAA
    GAAGGAAGGA CAACCCTGGG TCATGCATCT AAGGACATTC CCCTGCAGGG ACCTGGCATT
    GCTGCAGAAC ATTGCTACAT TGAAAACATC AGAGGAAACC TCACCCTATA CCCATGTGGA
    AATCCTTGTG CCATCGATGG ACTGCTCATC ACCCAACCGA CACGTCTCAC TCAAGGGTGT
    ATGGTCTGTC TGGGTCAGTC CACCTTCCTG AGATTTAACC ACCCAGCTGA AGCTCACTGG
    ATGAAAACCA TGATTCCTTC CACTGGGAGA AGCCCTGCGC CAGGCTTGTA CCCATCAGAT
    TCTTCTTCCT CCTTGCTAAA TGGTTCTCGT GATGCTGCAT ACCCGAGAAC TGTACCTTCC
    CATACCTCAC TAGTAAGCTC CATTGAAAAA AATCTTCAGG ACATCATGGA CTCGTTGGTT
    TTAGAAGGGC CTGCACCACT GAGCAGGAAG CTTTCCCAGA CCTCACTTAA CCATATCACC
    CCTATGACCA ATGGAGAGAA CAGACGGTAC TTGCTTTCAT CTCCAACAAG TCCACCTTAC
    TCAAACTCAG GTTATGACAA TACCTCTCCA CTGTCTTCTC CTGGAAGTGG AAGTCTCACT
    AGCCCCTCTC CGGGTCAAGA TGCAGGAACG CCAACAGCAC CCCCTGTGGT GCCACTTCGA
    ACTTCTAGCT TTAGCCATTC CTTGCCACGA CCAGTTCCTA CCTATGGAGG AAGCAACCTT
    GACTTGCGTT CTAATCGCAA CACAGGTGGT GGGTCTCTGC TTAGTCCTTC AAGCCCAGCA
    ACAGGAAGCA GGATGGGGGG ACTAAGTGTG CCAAGCAGTC CACTCCTAGG TAGTAAGTTT
    CATCACCAGA TCACAGACCG CCCAACCAGC CCTTTTAGAC AGGTGGTAGG ACCAACACGG
    AGTCTGCCAC CAGAGAGTCC AAGACTTGGG CGGAGGAACT TTGAAAGCAT GAGGGACCTG
    CCTCCACTTA GCCCTTCTCT GCCCCGAAGA AGTTCATCTA GCTCCATTAA ACCTGCTCCA
    GACAGCCCAT CTCGAGTTCC AGACAGCCCC AGCACTTTAG GGCGACGTCG GGTGGGAAGT
    GCTAGCTCTC CTGGCACAGA AAATAATGCA AGTACAAGGC AGATAAGGGA GCGCAGCCCA
    TCTCCCTCCT CTCTTCAAGA GACCTCTCGT CGACTAACTC CAGCTTATAG CCTGGGATCG
    CTTGGGACCC CTTCACAAAG CCCCAGGGCA CAAAGAAAAG GGCCTGTGCT ACGGGAGCGC
    AAGAACAGCA TCAGTGAAAT TTCTGGCAAC CAAGAGGAGC TAATGGACTA CCACAGAAGG
    CAGAGAGAAG AAAGACTTAG AGAGCAGGAA ATGGAAAGGC TGGAGCGGCA GAGACTGGAA
    ACCATTTTAA ACTTGTGTGC CGAATACAGT AAAACAGATG ATACAGCTCC CCCAGCTCGC
    CTAGATGAAA TAAGGAGCTT CCCCACTTTG GGGGGTACTG AGGAAACCAC TGCATCCCTC
    TTCACTCGTA GACCTTCTTG GGAGAGCACC AGAAGTACCA ACCTGGAGGG AACAGGCAAT
    AGGGTGAGAG CACAGGGAGA ACACAATGAG GAAGAGACTC AGAGAGAAGA GAACAGCAGT
    ACAGAGAGTA CCCAACATGA GGAAGATGCT GTAAGCCCAA CTGGGGCTGC AGAATTACTA
    GCCCTGGAGG AGCAACGCAG GCACACCCTG GGGCATGTGG ATCAGCTAAA GAATAGAGTG
    ACTGAATTGG AGGGTCAGTT GCAGGAAAGT GCAAGGGAGG CAGAGATGGA ACGTGCCCTT
    TTGCAAGGGG AACGGGAGGC TGAGCTGTCC CAGTTGCAGC ATGAGCAAAG AATGGTCCAG
    CAGCTGCAGG ACAGCCTGAC CTCTTTAGAA AACAACTTGC AGAAGGAGAA AGACAAGGCA
    CGTGAGAAAC TGGAGCAGGA GAGGATGGAG CTGCAGAGTA TTCAGCTTTC TCACCACGAC
    CTGCAGAGGC AGCTCCATAA CTGCCCTGAG GCTCTGCGGG AGCAGGTGCA GTGCCAGCTG
    ACACGGGAGT CTGAACTCCT GGAGACGAAG ATGAAGCTGT TTGAGGATCT GGAGTTCCAG
    CAGCTAGAAA AGGAAAGCCG CCTGGAAGAA GAAAGAGAGA CCACAAGTCA ACAACTTTTA
    CAGAGCAAGG GGGAATGCCA GCGAAGCATT GCACGCAGGA AGGAACGCCT GGCTGCTTTG
    GAGATGCAAG CCAATCAGAT TCGCCTCCAG GCAGCACAGG ATGCAGACCA TCTTGGCCAG
    GAAAGAAGCG CCATACTTTT ACAGCTACAC AAGGAGAAAG AGAAGCTGGT TCAGTTGGAG
    AGAAGATACC AGGCACTAAC AGGACGACGG GGATTCTCAA CTGGATCAGC TGCTCTCCGA
    GAGTACATCA CTTTAGATCA GCTGCAGGAT ATTCTGGAAA GCATGCCCAC AGGTTCTGCT
    CCAACTGCTG TGGCAATGGC TGGCACTGAT ATCAACTCTG CCTCTTGTGC TCCTTCACCT
    GACGACCCTC CTCCTGTACA TCTCTCGTCT TCATCTTCTT CCTCTCCCTC CATTTCTCTG
    GAGGTGAATT CCCCAACAAC AGAAGGTCAC TCTGCCCCCC TAGCACCTGA TTTGGATGCC
    TATTACCAAG ATTTAGTAAC TGCACCTTCT GCCTCTTCTT CTCCATCTTC TTCCCCTCCT
    CCACTCCCTG CTAAATCCCA CTCATCACGT AAGGCTTTGC AGATTTCTCG CTCTCGGACA
    GAAATTGAGG GTGGAGGCAC TCTTCCACGG ATGAAAACAG TCTCCCCCGC TTCTTCTCAC
    TTTTCAGTGG CCACTCTGGG CCGTAGTGTT TCCCCAAAGA ATGCCCATCT GACTCAGAAT
    GGCACAGGCA GCCTCTCCAG GGGATTGGCA GTAACCATGC AAGACATTCA AAGCAAGAGA
    CAGCTGGCAC TGCAACAAAA AGGTCAACAA GTGATAGAGG AGCAGCGCAG ACGTCTTGCA
    GAGTTGAAAC AGAGAGCTGC AGTTGAGGCA CAATCTCAGT GGGAATCTCT CCATGGAGCC
    CCTAATCAAG GGTACACGCC TATAATTCAC CACTCCATTC TACACCATCA GCGGCCTCTC
    CATTCTCGTG AAGATGACCC TACCCATGGA TTTGACACTC TCAGTCTGGA GAGTTCTGAT
    AGTTTGGACA CTAGTGTTTC TACAGGAAAC TCTGCATGTT CCCCTGATAA CATGTCAAGT
    GCTAGTGGGT TAGACATGCT AAAGATAGAG GAAATGGAAA GAATGCTTCT AGAAGCTCAT
    GCAGAGCGTG CCAGGCTTGT AGAATCCAGG GAGCGTGAGG TGGAGGCACG CAGAAAAGCA
    CTAGAAGATG AGAGGCGAAG GAGAGAGGAG CTGGAAAGGC GTCTTCAAGA TGAGACAGCT
    CACAGGCAAA GACTAGTAGA GAAGGAGGTC AAGATGAGGG AGAAGCATTT TTCACAGGCT
    CGTCCTCTTA CACGCTATCT GCCAATCCGG AAGGAAGACT TTGATCTCCG AGCCCATGTT
    GAGTCCTCAG GCCATGCAGT AGATGCCTGC CCTCACATCA TTTTAAGTGA GAAGATGTGT
    CGTGGCTTCC TGACAAAAAT GGGTGGAAAG ATAAAATCAT GGAAGAAGCG CTGGTTTGTT
    TTTGATCGCC TGAAACGGAC CCTGTCCTAT TATGTAGATA AACATGAGGC AAAGCTGAAA
    GGAGTTATAT ATTTCCAGGC CATAGAAGAG GTTTATTATG ACCACCTCAG GAGTGCAGCC
    AAGAGTCCTA ACCCAGCTCT AACATTCTGT GTCAAGACTC ACGATCGCCT GTATTACATG
    GTAGCTCCTT CCCCAGAGGC TATGCGGATA TGGATGGATG TCATTGTAAC TGGGGCAGAG
    GGTTACACTC AGTTCATGAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32404
    Clone ID Related Accession (Same CDS sequence) XM_012966165.3 , XM_012966165.2
    Accession Version XM_012966165.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4560bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pleckstrin homology-like domain family B member 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966165.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA
    GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG
    CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT
    CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA
    GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA
    ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT
    GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA
    ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA
    GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA
    TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA
    GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT
    CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC
    TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA
    CAAAAAGACA GTCTGAGCAC AGAAGATTCT CTTCCATCTG AAGTTGAAGG TCAACAAGTG
    ATAGAGGAGC AGCGCAGACG TCTTGCAGAG TTGAAACAGA GAGCTGCAGT TGAGGCACAA
    TCTCAGTGGG AATCTCTCCA TGGAGCCCCT AATCAAGGGT ACACGCCTAT AATTCACCAC
    TCCATTCTAC ACCATCAGCG GCCTCTCCAT TCTCGTGAAG ATGACCCTAC CCATGGATTT
    GACACTCTCA GTCTGGAGAG TTCTGATAGT TTGGACACTA GTGTTTCTAC AGGAAACTCT
    GCATGTTCCC CTGATAACAT GTCAAGTGCT AGTGGGTTAG ACATGCTAAA GATAGAGGAA
    ATGGAAAGAA TGCTTCTAGA AGCTCATGCA GAGCGTGCCA GGCTTGTAGA ATCCAGGGAG
    CGTGAGGTGG AGGCACGCAG AAAAGCACTA GAAGATGAGA GGCGAAGGAG AGAGGAGCTG
    GAAAGGCGTC TTCAAGATGA GACAGCTCAC AGGCAAAGAC TAGTAGAGAA GGAGGTCAAG
    ATGAGGGAGA AGCATTTTTC ACAGGCTCGT CCTCTTACAC GCTATCTGCC AATCCGGAAG
    GAAGACTTTG ATCTCCGAGC CCATGTTGAG TCCTCAGGCC ATGCAGTAGA TGCCTGCCCT
    CACATCATTT TAAGTGAGAA GATGTGTCGT GGCTTCCTGA CAAAAATGGG TGGAAAGATA
    AAATCATGGA AGAAGCGCTG GTTTGTTTTT GATCGCCTGA AACGGACCCT GTCCTATTAT
    GTAGATAAAC ATGAGGCAAA GCTGAAAGGA GTTATATATT TCCAGGCCAT AGAAGAGGTT
    TATTATGACC ACCTCAGGAG TGCAGCCAAG AAAGGACTTT TTAACCTCAG CCTGGCCACT
    CAGATATCCG ATTTTCTTTC TGTGCCCCTG TGGAAGGAGA GTCCTAACCC AGCTCTAACA
    TTCTGTGTCA AGACTCACGA TCGCCTGTAT TACATGGTAG CTCCTTCCCC AGAGGCTATG
    CGGATATGGA TGGATGTCAT TGTAACTGGG GCAGAGGGTT ACACTCAGTT CATGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821619.1
    CDS64..4623
    Translation

    Target ORF information:

    RefSeq Version XM_012966165.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966165.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA
    GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG
    CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT
    CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA
    GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA
    ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT
    GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA
    ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA
    GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA
    TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA
    GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT
    CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC
    TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA
    CAAAAAGACA GTCTGAGCAC AGAAGATTCT CTTCCATCTG AAGTTGAAGG TCAACAAGTG
    ATAGAGGAGC AGCGCAGACG TCTTGCAGAG TTGAAACAGA GAGCTGCAGT TGAGGCACAA
    TCTCAGTGGG AATCTCTCCA TGGAGCCCCT AATCAAGGGT ACACGCCTAT AATTCACCAC
    TCCATTCTAC ACCATCAGCG GCCTCTCCAT TCTCGTGAAG ATGACCCTAC CCATGGATTT
    GACACTCTCA GTCTGGAGAG TTCTGATAGT TTGGACACTA GTGTTTCTAC AGGAAACTCT
    GCATGTTCCC CTGATAACAT GTCAAGTGCT AGTGGGTTAG ACATGCTAAA GATAGAGGAA
    ATGGAAAGAA TGCTTCTAGA AGCTCATGCA GAGCGTGCCA GGCTTGTAGA ATCCAGGGAG
    CGTGAGGTGG AGGCACGCAG AAAAGCACTA GAAGATGAGA GGCGAAGGAG AGAGGAGCTG
    GAAAGGCGTC TTCAAGATGA GACAGCTCAC AGGCAAAGAC TAGTAGAGAA GGAGGTCAAG
    ATGAGGGAGA AGCATTTTTC ACAGGCTCGT CCTCTTACAC GCTATCTGCC AATCCGGAAG
    GAAGACTTTG ATCTCCGAGC CCATGTTGAG TCCTCAGGCC ATGCAGTAGA TGCCTGCCCT
    CACATCATTT TAAGTGAGAA GATGTGTCGT GGCTTCCTGA CAAAAATGGG TGGAAAGATA
    AAATCATGGA AGAAGCGCTG GTTTGTTTTT GATCGCCTGA AACGGACCCT GTCCTATTAT
    GTAGATAAAC ATGAGGCAAA GCTGAAAGGA GTTATATATT TCCAGGCCAT AGAAGAGGTT
    TATTATGACC ACCTCAGGAG TGCAGCCAAG AAAGGACTTT TTAACCTCAG CCTGGCCACT
    CAGATATCCG ATTTTCTTTC TGTGCCCCTG TGGAAGGAGA GTCCTAACCC AGCTCTAACA
    TTCTGTGTCA AGACTCACGA TCGCCTGTAT TACATGGTAG CTCCTTCCCC AGAGGCTATG
    CGGATATGGA TGGATGTCAT TGTAACTGGG GCAGAGGGTT ACACTCAGTT CATGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32405
    Clone ID Related Accession (Same CDS sequence) XM_012966167.2 , XM_012966167.3
    Accession Version XM_012966167.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4557bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pleckstrin homology-like domain family B member 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966167.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG GAAGATGCTG TAAGCCCAAC TGGGGCTGCA
    GAATTACTAG CCCTGGAGGA GCAACGCAGG CACACCCTGG GGCATGTGGA TCAGCTAAAG
    AATAGAGTGA CTGAATTGGA GGGTCAGTTG CAGGAAAGTG CAAGGGAGGC AGAGATGGAA
    CGTGCCCTTT TGCAAGGGGA ACGGGAGGCT GAGCTGTCCC AGTTGCAGCA TGAGCAAAGA
    ATGGTCCAGC AGCTGCAGGA CAGCCTGACC TCTTTAGAAA ACAACTTGCA GAAGGAGAAA
    GACAAGGCAC GTGAGAAACT GGAGCAGGAG AGGATGGAGC TGCAGAGTAT TCAGCTTTCT
    CACCACGACC TGCAGAGGCA GCTCCATAAC TGCCCTGAGG CTCTGCGGGA GCAGGTGCAG
    TGCCAGCTGA CACGGGAGTC TGAACTCCTG GAGACGAAGA TGAAGCTGTT TGAGGATCTG
    GAGTTCCAGC AGCTAGAAAA GGAAAGCCGC CTGGAAGAAG AAAGAGAGAC CACAAGTCAA
    CAACTTTTAC AGAGCAAGGG GGAATGCCAG CGAAGCATTG CACGCAGGAA GGAACGCCTG
    GCTGCTTTGG AGATGCAAGC CAATCAGATT CGCCTCCAGG CAGCACAGGA TGCAGACCAT
    CTTGGCCAGG AAAGAAGCGC CATACTTTTA CAGCTACACA AGGAGAAAGA GAAGCTGGTT
    CAGTTGGAGA GAAGATACCA GGCACTAACA GGACGACGGG GATTCTCAAC TGGATCAGCT
    GCTCTCCGAG AGGATGCTCT TCAAATCTAT GAGACTAGAG ACTGCTTACT CGCTCAAGCT
    GATGTTTCTT GCACACCTGC TATCCCTTCT GCCTGCTCTC ACTCCCCTTC CCGGCAGCAA
    GAGGAATACC TGTGCCTCTC TGATGTTCTC CGCTTGTACA GCAGCTCGCC TCTTGTTCCT
    GCTTTTCCCC TTCTCTCTGC TGCAGAGCCC ACCGCCGCCC TTGAGTACAT CACTTTAGAT
    CAGCTGCAGG ATATTCTGGA AAGCATGCCC ACAGGTTCTG CTCCAACTGC TGTGGCAATG
    GCTGGCACTG ATATCAACTC TGCCTCTTGT GCTCCTTCAC CTGACGACCC TCCTCCTGTA
    CATCTCTCGT CTTCATCTTC TTCCTCTCCC TCCATTTCTC TGGAGGTGAA TTCCCCAACA
    ACAGAAGGTC ACTCTGCCCC CCTAGCACCT GATTTGGATG CCTATTACCA AGATTTAGTA
    ACTGCACCTT CTGCCTCTTC TTCTCCATCT TCTTCCCCTC CTCCACTCCC TGCTAAATCC
    CACTCATCAC GTAAGGCTTT GCAGATTTCT CGCTCTCGGA CAGAAATTGA GGGTGGAGGC
    ACTCTTCCAC GGATGAAAAC AGTCTCCCCC GCTTCTTCTC ACTTTTCAGT GGCCACTCTG
    GGCCGTAGTG TTTCCCCAAA GAATGCCCAT CTGACTCAGA ATGGCACAGG CAGCCTCTCC
    AGGGGATTGG CAGTAACCAT GCAAGACATT CAAAGCAAGA GACAGCTGGC ACTGCAACAA
    AAAGACAGTC TGAGCACAGA AGATTCTCTT CCATCTGAAG TTGAAGGTCA ACAAGTGATA
    GAGGAGCAGC GCAGACGTCT TGCAGAGTTG AAACAGAGAG CTGCAGTTGA GGCACAATCT
    CAGTGGGAAT CTCTCCATGG AGCCCCTAAT CAAGGGTACA CGCCTATAAT TCACCACTCC
    ATTCTACACC ATCAGCGGCC TCTCCATTCT CGTGAAGATG ACCCTACCCA TGGATTTGAC
    ACTCTCAGTC TGGAGAGTTC TGATAGTTTG GACACTAGTG TTTCTACAGG AAACTCTGCA
    TGTTCCCCTG ATAACATGTC AAGTGCTAGT GGGTTAGACA TGCTAAAGAT AGAGGAAATG
    GAAAGAATGC TTCTAGAAGC TCATGCAGAG CGTGCCAGGC TTGTAGAATC CAGGGAGCGT
    GAGGTGGAGG CACGCAGAAA AGCACTAGAA GATGAGAGGC GAAGGAGAGA GGAGCTGGAA
    AGGCGTCTTC AAGATGAGAC AGCTCACAGG CAAAGACTAG TAGAGAAGGA GGTCAAGATG
    AGGGAGAAGC ATTTTTCACA GGCTCGTCCT CTTACACGCT ATCTGCCAAT CCGGAAGGAA
    GACTTTGATC TCCGAGCCCA TGTTGAGTCC TCAGGCCATG CAGTAGATGC CTGCCCTCAC
    ATCATTTTAA GTGAGAAGAT GTGTCGTGGC TTCCTGACAA AAATGGGTGG AAAGATAAAA
    TCATGGAAGA AGCGCTGGTT TGTTTTTGAT CGCCTGAAAC GGACCCTGTC CTATTATGTA
    GATAAACATG AGGCAAAGCT GAAAGGAGTT ATATATTTCC AGGCCATAGA AGAGGTTTAT
    TATGACCACC TCAGGAGTGC AGCCAAGAAA GGACTTTTTA ACCTCAGCCT GGCCACTCAG
    ATATCCGATT TTCTTTCTGT GCCCCTGTGG AAGGAGAGTC CTAACCCAGC TCTAACATTC
    TGTGTCAAGA CTCACGATCG CCTGTATTAC ATGGTAGCTC CTTCCCCAGA GGCTATGCGG
    ATATGGATGG ATGTCATTGT AACTGGGGCA GAGGGTTACA CTCAGTTCAT GAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821621.1
    CDS63..4619
    Translation

    Target ORF information:

    RefSeq Version XM_012966167.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966167.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG GAAGATGCTG TAAGCCCAAC TGGGGCTGCA
    GAATTACTAG CCCTGGAGGA GCAACGCAGG CACACCCTGG GGCATGTGGA TCAGCTAAAG
    AATAGAGTGA CTGAATTGGA GGGTCAGTTG CAGGAAAGTG CAAGGGAGGC AGAGATGGAA
    CGTGCCCTTT TGCAAGGGGA ACGGGAGGCT GAGCTGTCCC AGTTGCAGCA TGAGCAAAGA
    ATGGTCCAGC AGCTGCAGGA CAGCCTGACC TCTTTAGAAA ACAACTTGCA GAAGGAGAAA
    GACAAGGCAC GTGAGAAACT GGAGCAGGAG AGGATGGAGC TGCAGAGTAT TCAGCTTTCT
    CACCACGACC TGCAGAGGCA GCTCCATAAC TGCCCTGAGG CTCTGCGGGA GCAGGTGCAG
    TGCCAGCTGA CACGGGAGTC TGAACTCCTG GAGACGAAGA TGAAGCTGTT TGAGGATCTG
    GAGTTCCAGC AGCTAGAAAA GGAAAGCCGC CTGGAAGAAG AAAGAGAGAC CACAAGTCAA
    CAACTTTTAC AGAGCAAGGG GGAATGCCAG CGAAGCATTG CACGCAGGAA GGAACGCCTG
    GCTGCTTTGG AGATGCAAGC CAATCAGATT CGCCTCCAGG CAGCACAGGA TGCAGACCAT
    CTTGGCCAGG AAAGAAGCGC CATACTTTTA CAGCTACACA AGGAGAAAGA GAAGCTGGTT
    CAGTTGGAGA GAAGATACCA GGCACTAACA GGACGACGGG GATTCTCAAC TGGATCAGCT
    GCTCTCCGAG AGGATGCTCT TCAAATCTAT GAGACTAGAG ACTGCTTACT CGCTCAAGCT
    GATGTTTCTT GCACACCTGC TATCCCTTCT GCCTGCTCTC ACTCCCCTTC CCGGCAGCAA
    GAGGAATACC TGTGCCTCTC TGATGTTCTC CGCTTGTACA GCAGCTCGCC TCTTGTTCCT
    GCTTTTCCCC TTCTCTCTGC TGCAGAGCCC ACCGCCGCCC TTGAGTACAT CACTTTAGAT
    CAGCTGCAGG ATATTCTGGA AAGCATGCCC ACAGGTTCTG CTCCAACTGC TGTGGCAATG
    GCTGGCACTG ATATCAACTC TGCCTCTTGT GCTCCTTCAC CTGACGACCC TCCTCCTGTA
    CATCTCTCGT CTTCATCTTC TTCCTCTCCC TCCATTTCTC TGGAGGTGAA TTCCCCAACA
    ACAGAAGGTC ACTCTGCCCC CCTAGCACCT GATTTGGATG CCTATTACCA AGATTTAGTA
    ACTGCACCTT CTGCCTCTTC TTCTCCATCT TCTTCCCCTC CTCCACTCCC TGCTAAATCC
    CACTCATCAC GTAAGGCTTT GCAGATTTCT CGCTCTCGGA CAGAAATTGA GGGTGGAGGC
    ACTCTTCCAC GGATGAAAAC AGTCTCCCCC GCTTCTTCTC ACTTTTCAGT GGCCACTCTG
    GGCCGTAGTG TTTCCCCAAA GAATGCCCAT CTGACTCAGA ATGGCACAGG CAGCCTCTCC
    AGGGGATTGG CAGTAACCAT GCAAGACATT CAAAGCAAGA GACAGCTGGC ACTGCAACAA
    AAAGACAGTC TGAGCACAGA AGATTCTCTT CCATCTGAAG TTGAAGGTCA ACAAGTGATA
    GAGGAGCAGC GCAGACGTCT TGCAGAGTTG AAACAGAGAG CTGCAGTTGA GGCACAATCT
    CAGTGGGAAT CTCTCCATGG AGCCCCTAAT CAAGGGTACA CGCCTATAAT TCACCACTCC
    ATTCTACACC ATCAGCGGCC TCTCCATTCT CGTGAAGATG ACCCTACCCA TGGATTTGAC
    ACTCTCAGTC TGGAGAGTTC TGATAGTTTG GACACTAGTG TTTCTACAGG AAACTCTGCA
    TGTTCCCCTG ATAACATGTC AAGTGCTAGT GGGTTAGACA TGCTAAAGAT AGAGGAAATG
    GAAAGAATGC TTCTAGAAGC TCATGCAGAG CGTGCCAGGC TTGTAGAATC CAGGGAGCGT
    GAGGTGGAGG CACGCAGAAA AGCACTAGAA GATGAGAGGC GAAGGAGAGA GGAGCTGGAA
    AGGCGTCTTC AAGATGAGAC AGCTCACAGG CAAAGACTAG TAGAGAAGGA GGTCAAGATG
    AGGGAGAAGC ATTTTTCACA GGCTCGTCCT CTTACACGCT ATCTGCCAAT CCGGAAGGAA
    GACTTTGATC TCCGAGCCCA TGTTGAGTCC TCAGGCCATG CAGTAGATGC CTGCCCTCAC
    ATCATTTTAA GTGAGAAGAT GTGTCGTGGC TTCCTGACAA AAATGGGTGG AAAGATAAAA
    TCATGGAAGA AGCGCTGGTT TGTTTTTGAT CGCCTGAAAC GGACCCTGTC CTATTATGTA
    GATAAACATG AGGCAAAGCT GAAAGGAGTT ATATATTTCC AGGCCATAGA AGAGGTTTAT
    TATGACCACC TCAGGAGTGC AGCCAAGAAA GGACTTTTTA ACCTCAGCCT GGCCACTCAG
    ATATCCGATT TTCTTTCTGT GCCCCTGTGG AAGGAGAGTC CTAACCCAGC TCTAACATTC
    TGTGTCAAGA CTCACGATCG CCTGTATTAC ATGGTAGCTC CTTCCCCAGA GGCTATGCGG
    ATATGGATGG ATGTCATTGT AACTGGGGCA GAGGGTTACA CTCAGTTCAT GAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32406
    Clone ID Related Accession (Same CDS sequence) XM_012966168.2 , XM_012966168.3
    Accession Version XM_012966168.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4539bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pleckstrin homology-like domain family B member 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966168.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGAG
    TCTGAACTCC TGGAGACGAA GATGAAGCTG TTTGAGGATC TGGAGTTCCA GCAGCTAGAA
    AAGGAAAGCC GCCTGGAAGA AGAAAGAGAG ACCACAAGTC AACAACTTTT ACAGAGCAAG
    GGGGAATGCC AGCGAAGCAT TGCACGCAGG AAGGAACGCC TGGCTGCTTT GGAGATGCAA
    GCCAATCAGA TTCGCCTCCA GGCAGCACAG GATGCAGACC ATCTTGGCCA GGAAAGAAGC
    GCCATACTTT TACAGCTACA CAAGGAGAAA GAGAAGCTGG TTCAGTTGGA GAGAAGATAC
    CAGGCACTAA CAGGACGACG GGGATTCTCA ACTGGATCAG CTGCTCTCCG AGAGGATGCT
    CTTCAAATCT ATGAGACTAG AGACTGCTTA CTCGCTCAAG CTGATGTTTC TTGCACACCT
    GCTATCCCTT CTGCCTGCTC TCACTCCCCT TCCCGGCAGC AAGAGGAATA CCTGTGCCTC
    TCTGATGTTC TCCGCTTGTA CAGCAGCTCG CCTCTTGTTC CTGCTTTTCC CCTTCTCTCT
    GCTGCAGAGC CCACCGCCGC CCTTGAGTAC ATCACTTTAG ATCAGCTGCA GGATATTCTG
    GAAAGCATGC CCACAGGTTC TGCTCCAACT GCTGTGGCAA TGGCTGGCAC TGATATCAAC
    TCTGCCTCTT GTGCTCCTTC ACCTGACGAC CCTCCTCCTG TACATCTCTC GTCTTCATCT
    TCTTCCTCTC CCTCCATTTC TCTGGAGGTG AATTCCCCAA CAACAGAAGG TCACTCTGCC
    CCCCTAGCAC CTGATTTGGA TGCCTATTAC CAAGATTTAG TAACTGCACC TTCTGCCTCT
    TCTTCTCCAT CTTCTTCCCC TCCTCCACTC CCTGCTAAAT CCCACTCATC ACGTAAGGCT
    TTGCAGATTT CTCGCTCTCG GACAGAAATT GAGGGTGGAG GCACTCTTCC ACGGATGAAA
    ACAGTCTCCC CCGCTTCTTC TCACTTTTCA GTGGCCACTC TGGGCCGTAG TGTTTCCCCA
    AAGAATGCCC ATCTGACTCA GAATGGCACA GGCAGCCTCT CCAGGGGATT GGCAGTAACC
    ATGCAAGACA TTCAAAGCAA GAGACAGCTG GCACTGCAAC AAAAAGACAG TCTGAGCACA
    GAAGATTCTC TTCCATCTGA AGTTGAAGGT CAACAAGTGA TAGAGGAGCA GCGCAGACGT
    CTTGCAGAGT TGAAACAGAG AGCTGCAGTT GAGGCACAAT CTCAGTGGGA ATCTCTCCAT
    GGAGCCCCTA ATCAAGGGTA CACGCCTATA ATTCACCACT CCATTCTACA CCATCAGCGG
    CCTCTCCATT CTCGTGAAGA TGACCCTACC CATGGATTTG ACACTCTCAG TCTGGAGAGT
    TCTGATAGTT TGGACACTAG TGTTTCTACA GGAAACTCTG CATGTTCCCC TGATAACATG
    TCAAGTGCTA GTGGGTTAGA CATGCTAAAG ATAGAGGAAA TGGAAAGAAT GCTTCTAGAA
    GCTCATGCAG AGCGTGCCAG GCTTGTAGAA TCCAGGGAGC GTGAGGTGGA GGCACGCAGA
    AAAGCACTAG AAGATGAGAG GCGAAGGAGA GAGGAGCTGG AAAGGCGTCT TCAAGATGAG
    ACAGCTCACA GGCAAAGACT AGTAGAGAAG GAGGTCAAGA TGAGGGAGAA GCATTTTTCA
    CAGGCTCGTC CTCTTACACG CTATCTGCCA ATCCGGAAGG AAGACTTTGA TCTCCGAGCC
    CATGTTGAGT CCTCAGGCCA TGCAGTAGAT GCCTGCCCTC ACATCATTTT AAGTGAGAAG
    ATGTGTCGTG GCTTCCTGAC AAAAATGGGT GGAAAGATAA AATCATGGAA GAAGCGCTGG
    TTTGTTTTTG ATCGCCTGAA ACGGACCCTG TCCTATTATG TAGATAAACA TGAGGCAAAG
    CTGAAAGGAG TTATATATTT CCAGGCCATA GAAGAGGTTT ATTATGACCA CCTCAGGAGT
    GCAGCCAAGA AAGGACTTTT TAACCTCAGC CTGGCCACTC AGATATCCGA TTTTCTTTCT
    GTGCCCCTGT GGAAGGAGAG TCCTAACCCA GCTCTAACAT TCTGTGTCAA GACTCACGAT
    CGCCTGTATT ACATGGTAGC TCCTTCCCCA GAGGCTATGC GGATATGGAT GGATGTCATT
    GTAACTGGGG CAGAGGGTTA CACTCAGTTC ATGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821622.1
    CDS64..4602
    Translation

    Target ORF information:

    RefSeq Version XM_012966168.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966168.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGAG
    TCTGAACTCC TGGAGACGAA GATGAAGCTG TTTGAGGATC TGGAGTTCCA GCAGCTAGAA
    AAGGAAAGCC GCCTGGAAGA AGAAAGAGAG ACCACAAGTC AACAACTTTT ACAGAGCAAG
    GGGGAATGCC AGCGAAGCAT TGCACGCAGG AAGGAACGCC TGGCTGCTTT GGAGATGCAA
    GCCAATCAGA TTCGCCTCCA GGCAGCACAG GATGCAGACC ATCTTGGCCA GGAAAGAAGC
    GCCATACTTT TACAGCTACA CAAGGAGAAA GAGAAGCTGG TTCAGTTGGA GAGAAGATAC
    CAGGCACTAA CAGGACGACG GGGATTCTCA ACTGGATCAG CTGCTCTCCG AGAGGATGCT
    CTTCAAATCT ATGAGACTAG AGACTGCTTA CTCGCTCAAG CTGATGTTTC TTGCACACCT
    GCTATCCCTT CTGCCTGCTC TCACTCCCCT TCCCGGCAGC AAGAGGAATA CCTGTGCCTC
    TCTGATGTTC TCCGCTTGTA CAGCAGCTCG CCTCTTGTTC CTGCTTTTCC CCTTCTCTCT
    GCTGCAGAGC CCACCGCCGC CCTTGAGTAC ATCACTTTAG ATCAGCTGCA GGATATTCTG
    GAAAGCATGC CCACAGGTTC TGCTCCAACT GCTGTGGCAA TGGCTGGCAC TGATATCAAC
    TCTGCCTCTT GTGCTCCTTC ACCTGACGAC CCTCCTCCTG TACATCTCTC GTCTTCATCT
    TCTTCCTCTC CCTCCATTTC TCTGGAGGTG AATTCCCCAA CAACAGAAGG TCACTCTGCC
    CCCCTAGCAC CTGATTTGGA TGCCTATTAC CAAGATTTAG TAACTGCACC TTCTGCCTCT
    TCTTCTCCAT CTTCTTCCCC TCCTCCACTC CCTGCTAAAT CCCACTCATC ACGTAAGGCT
    TTGCAGATTT CTCGCTCTCG GACAGAAATT GAGGGTGGAG GCACTCTTCC ACGGATGAAA
    ACAGTCTCCC CCGCTTCTTC TCACTTTTCA GTGGCCACTC TGGGCCGTAG TGTTTCCCCA
    AAGAATGCCC ATCTGACTCA GAATGGCACA GGCAGCCTCT CCAGGGGATT GGCAGTAACC
    ATGCAAGACA TTCAAAGCAA GAGACAGCTG GCACTGCAAC AAAAAGACAG TCTGAGCACA
    GAAGATTCTC TTCCATCTGA AGTTGAAGGT CAACAAGTGA TAGAGGAGCA GCGCAGACGT
    CTTGCAGAGT TGAAACAGAG AGCTGCAGTT GAGGCACAAT CTCAGTGGGA ATCTCTCCAT
    GGAGCCCCTA ATCAAGGGTA CACGCCTATA ATTCACCACT CCATTCTACA CCATCAGCGG
    CCTCTCCATT CTCGTGAAGA TGACCCTACC CATGGATTTG ACACTCTCAG TCTGGAGAGT
    TCTGATAGTT TGGACACTAG TGTTTCTACA GGAAACTCTG CATGTTCCCC TGATAACATG
    TCAAGTGCTA GTGGGTTAGA CATGCTAAAG ATAGAGGAAA TGGAAAGAAT GCTTCTAGAA
    GCTCATGCAG AGCGTGCCAG GCTTGTAGAA TCCAGGGAGC GTGAGGTGGA GGCACGCAGA
    AAAGCACTAG AAGATGAGAG GCGAAGGAGA GAGGAGCTGG AAAGGCGTCT TCAAGATGAG
    ACAGCTCACA GGCAAAGACT AGTAGAGAAG GAGGTCAAGA TGAGGGAGAA GCATTTTTCA
    CAGGCTCGTC CTCTTACACG CTATCTGCCA ATCCGGAAGG AAGACTTTGA TCTCCGAGCC
    CATGTTGAGT CCTCAGGCCA TGCAGTAGAT GCCTGCCCTC ACATCATTTT AAGTGAGAAG
    ATGTGTCGTG GCTTCCTGAC AAAAATGGGT GGAAAGATAA AATCATGGAA GAAGCGCTGG
    TTTGTTTTTG ATCGCCTGAA ACGGACCCTG TCCTATTATG TAGATAAACA TGAGGCAAAG
    CTGAAAGGAG TTATATATTT CCAGGCCATA GAAGAGGTTT ATTATGACCA CCTCAGGAGT
    GCAGCCAAGA AAGGACTTTT TAACCTCAGC CTGGCCACTC AGATATCCGA TTTTCTTTCT
    GTGCCCCTGT GGAAGGAGAG TCCTAACCCA GCTCTAACAT TCTGTGTCAA GACTCACGAT
    CGCCTGTATT ACATGGTAGC TCCTTCCCCA GAGGCTATGC GGATATGGAT GGATGTCATT
    GTAACTGGGG CAGAGGGTTA CACTCAGTTC ATGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32407
    Clone ID Related Accession (Same CDS sequence) XM_012966169.3 , XM_012966169.2
    Accession Version XM_012966169.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4521bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pleckstrin homology-like domain family B member 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966169.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA
    GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG
    CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT
    CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA
    GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA
    ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT
    GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA
    ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA
    GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA
    TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA
    GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT
    CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC
    TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA
    CAAAAAGACA GTCTGAGCAC AGAAGATTCT CTTCCATCTG AAGTTGAAGG TCAACAAGTG
    ATAGAGGAGC AGCGCAGACG TCTTGCAGAG TTGAAACAGA GAGCTGCAGT TGAGGCACAA
    TCTCAGTGGG AATCTCTCCA TGGAGCCCCT AATCAAGGGT ACACGCCTAT AATTCACCAC
    TCCATTCTAC ACCATCAGCG GCCTCTCCAT TCTCGTGAAG ATGACCCTAC CCATGGATTT
    GACACTCTCA GTCTGGAGAG TTCTGATAGT TTGGACACTA GTGTTTCTAC AGGAAACTCT
    GCATGTTCCC CTGATAACAT GTCAAGTGCT AGTGGGTTAG ACATGCTAAA GATAGAGGAA
    ATGGAAAGAA TGCTTCTAGA AGCTCATGCA GAGCGTGCCA GGCTTGTAGA ATCCAGGGAG
    CGTGAGGTGG AGGCACGCAG AAAAGCACTA GAAGATGAGA GGCGAAGGAG AGAGGAGCTG
    GAAAGGCGTC TTCAAGATGA GACAGCTCAC AGGCAAAGAC TAGTAGAGAA GGAGGTCAAG
    ATGAGGGAGA AGCATTTTTC ACAGGCTCGT CCTCTTACAC GCTATCTGCC AATCCGGAAG
    GAAGACTTTG ATCTCCGAGC CCATGTTGAG TCCTCAGGCC ATGCAGTAGA TGCCTGCCCT
    CACATCATTT TAAGTGAGAA GATGTGTCGT GGCTTCCTGA CAAAAATGGG TGGAAAGATA
    AAATCATGGA AGAAGCGCTG GTTTGTTTTT GATCGCCTGA AACGGACCCT GTCCTATTAT
    GTAGATAAAC ATGAGGCAAA GCTGAAAGGA GTTATATATT TCCAGGCCAT AGAAGAGGTT
    TATTATGACC ACCTCAGGAG TGCAGCCAAG AAAGGACTTT TTAACCTCAG CCTGGCCACT
    AGTCCTAACC CAGCTCTAAC ATTCTGTGTC AAGACTCACG ATCGCCTGTA TTACATGGTA
    GCTCCTTCCC CAGAGGCTAT GCGGATATGG ATGGATGTCA TTGTAACTGG GGCAGAGGGT
    TACACTCAGT TCATGAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821623.1
    CDS63..4583
    Translation

    Target ORF information:

    RefSeq Version XM_012966169.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966169.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA
    GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG
    CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT
    CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA
    GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA
    ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT
    GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA
    ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA
    GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA
    TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA
    GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT
    CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC
    TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA
    CAAAAAGACA GTCTGAGCAC AGAAGATTCT CTTCCATCTG AAGTTGAAGG TCAACAAGTG
    ATAGAGGAGC AGCGCAGACG TCTTGCAGAG TTGAAACAGA GAGCTGCAGT TGAGGCACAA
    TCTCAGTGGG AATCTCTCCA TGGAGCCCCT AATCAAGGGT ACACGCCTAT AATTCACCAC
    TCCATTCTAC ACCATCAGCG GCCTCTCCAT TCTCGTGAAG ATGACCCTAC CCATGGATTT
    GACACTCTCA GTCTGGAGAG TTCTGATAGT TTGGACACTA GTGTTTCTAC AGGAAACTCT
    GCATGTTCCC CTGATAACAT GTCAAGTGCT AGTGGGTTAG ACATGCTAAA GATAGAGGAA
    ATGGAAAGAA TGCTTCTAGA AGCTCATGCA GAGCGTGCCA GGCTTGTAGA ATCCAGGGAG
    CGTGAGGTGG AGGCACGCAG AAAAGCACTA GAAGATGAGA GGCGAAGGAG AGAGGAGCTG
    GAAAGGCGTC TTCAAGATGA GACAGCTCAC AGGCAAAGAC TAGTAGAGAA GGAGGTCAAG
    ATGAGGGAGA AGCATTTTTC ACAGGCTCGT CCTCTTACAC GCTATCTGCC AATCCGGAAG
    GAAGACTTTG ATCTCCGAGC CCATGTTGAG TCCTCAGGCC ATGCAGTAGA TGCCTGCCCT
    CACATCATTT TAAGTGAGAA GATGTGTCGT GGCTTCCTGA CAAAAATGGG TGGAAAGATA
    AAATCATGGA AGAAGCGCTG GTTTGTTTTT GATCGCCTGA AACGGACCCT GTCCTATTAT
    GTAGATAAAC ATGAGGCAAA GCTGAAAGGA GTTATATATT TCCAGGCCAT AGAAGAGGTT
    TATTATGACC ACCTCAGGAG TGCAGCCAAG AAAGGACTTT TTAACCTCAG CCTGGCCACT
    AGTCCTAACC CAGCTCTAAC ATTCTGTGTC AAGACTCACG ATCGCCTGTA TTACATGGTA
    GCTCCTTCCC CAGAGGCTAT GCGGATATGG ATGGATGTCA TTGTAACTGG GGCAGAGGGT
    TACACTCAGT TCATGAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32408
    Clone ID Related Accession (Same CDS sequence) XM_012966170.3 , XM_012966170.2
    Accession Version XM_012966170.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4518bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pleckstrin homology-like domain family B member 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966170.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA
    GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG
    CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT
    CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA
    GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA
    ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT
    GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA
    ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA
    GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA
    TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA
    GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT
    CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC
    TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA
    CAAAAAGGTC AACAAGTGAT AGAGGAGCAG CGCAGACGTC TTGCAGAGTT GAAACAGAGA
    GCTGCAGTTG AGGCACAATC TCAGTGGGAA TCTCTCCATG GAGCCCCTAA TCAAGGGTAC
    ACGCCTATAA TTCACCACTC CATTCTACAC CATCAGCGGC CTCTCCATTC TCGTGAAGAT
    GACCCTACCC ATGGATTTGA CACTCTCAGT CTGGAGAGTT CTGATAGTTT GGACACTAGT
    GTTTCTACAG GAAACTCTGC ATGTTCCCCT GATAACATGT CAAGTGCTAG TGGGTTAGAC
    ATGCTAAAGA TAGAGGAAAT GGAAAGAATG CTTCTAGAAG CTCATGCAGA GCGTGCCAGG
    CTTGTAGAAT CCAGGGAGCG TGAGGTGGAG GCACGCAGAA AAGCACTAGA AGATGAGAGG
    CGAAGGAGAG AGGAGCTGGA AAGGCGTCTT CAAGATGAGA CAGCTCACAG GCAAAGACTA
    GTAGAGAAGG AGGTCAAGAT GAGGGAGAAG CATTTTTCAC AGGCTCGTCC TCTTACACGC
    TATCTGCCAA TCCGGAAGGA AGACTTTGAT CTCCGAGCCC ATGTTGAGTC CTCAGGCCAT
    GCAGTAGATG CCTGCCCTCA CATCATTTTA AGTGAGAAGA TGTGTCGTGG CTTCCTGACA
    AAAATGGGTG GAAAGATAAA ATCATGGAAG AAGCGCTGGT TTGTTTTTGA TCGCCTGAAA
    CGGACCCTGT CCTATTATGT AGATAAACAT GAGGCAAAGC TGAAAGGAGT TATATATTTC
    CAGGCCATAG AAGAGGTTTA TTATGACCAC CTCAGGAGTG CAGCCAAGAA AGGACTTTTT
    AACCTCAGCC TGGCCACTCA GATATCCGAT TTTCTTTCTG TGCCCCTGTG GAAGGAGAGT
    CCTAACCCAG CTCTAACATT CTGTGTCAAG ACTCACGATC GCCTGTATTA CATGGTAGCT
    CCTTCCCCAG AGGCTATGCG GATATGGATG GATGTCATTG TAACTGGGGC AGAGGGTTAC
    ACTCAGTTCA TGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821624.1
    CDS63..4580
    Translation

    Target ORF information:

    RefSeq Version XM_012966170.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966170.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA
    GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG
    CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT
    CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA
    GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA
    ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT
    GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA
    ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA
    GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA
    TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA
    GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT
    CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC
    TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA
    CAAAAAGGTC AACAAGTGAT AGAGGAGCAG CGCAGACGTC TTGCAGAGTT GAAACAGAGA
    GCTGCAGTTG AGGCACAATC TCAGTGGGAA TCTCTCCATG GAGCCCCTAA TCAAGGGTAC
    ACGCCTATAA TTCACCACTC CATTCTACAC CATCAGCGGC CTCTCCATTC TCGTGAAGAT
    GACCCTACCC ATGGATTTGA CACTCTCAGT CTGGAGAGTT CTGATAGTTT GGACACTAGT
    GTTTCTACAG GAAACTCTGC ATGTTCCCCT GATAACATGT CAAGTGCTAG TGGGTTAGAC
    ATGCTAAAGA TAGAGGAAAT GGAAAGAATG CTTCTAGAAG CTCATGCAGA GCGTGCCAGG
    CTTGTAGAAT CCAGGGAGCG TGAGGTGGAG GCACGCAGAA AAGCACTAGA AGATGAGAGG
    CGAAGGAGAG AGGAGCTGGA AAGGCGTCTT CAAGATGAGA CAGCTCACAG GCAAAGACTA
    GTAGAGAAGG AGGTCAAGAT GAGGGAGAAG CATTTTTCAC AGGCTCGTCC TCTTACACGC
    TATCTGCCAA TCCGGAAGGA AGACTTTGAT CTCCGAGCCC ATGTTGAGTC CTCAGGCCAT
    GCAGTAGATG CCTGCCCTCA CATCATTTTA AGTGAGAAGA TGTGTCGTGG CTTCCTGACA
    AAAATGGGTG GAAAGATAAA ATCATGGAAG AAGCGCTGGT TTGTTTTTGA TCGCCTGAAA
    CGGACCCTGT CCTATTATGT AGATAAACAT GAGGCAAAGC TGAAAGGAGT TATATATTTC
    CAGGCCATAG AAGAGGTTTA TTATGACCAC CTCAGGAGTG CAGCCAAGAA AGGACTTTTT
    AACCTCAGCC TGGCCACTCA GATATCCGAT TTTCTTTCTG TGCCCCTGTG GAAGGAGAGT
    CCTAACCCAG CTCTAACATT CTGTGTCAAG ACTCACGATC GCCTGTATTA CATGGTAGCT
    CCTTCCCCAG AGGCTATGCG GATATGGATG GATGTCATTG TAACTGGGGC AGAGGGTTAC
    ACTCAGTTCA TGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32409
    Clone ID Related Accession (Same CDS sequence) XM_012966171.2 , XM_012966171.3
    Accession Version XM_012966171.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4491bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pleckstrin homology-like domain family B member 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966171.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA
    GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG
    CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT
    CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA
    GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA
    ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT
    GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA
    ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA
    GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA
    TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA
    GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT
    CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC
    TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA
    CAAAAAGACA GTCTGAGCAC AGAAGATTCT CTTCCATCTG AAGTTGAAGG TCAACAAGTG
    ATAGAGGAGC AGCGCAGACG TCTTGCAGAG TTGAAACAGA GAGCTGCAGT TGAGGCACAA
    TCTCAGTGGG AATCTCTCCA TGGAGCCCCT AATCAAGGGT ACACGCCTAT AATTCACCAC
    TCCATTCTAC ACCATCAGCG GCCTCTCCAT TCTCGTGAAG ATGACCCTAC CCATGGATTT
    GACACTCTCA GTCTGGAGAG TTCTGATAGT TTGGACACTA GTGTTTCTAC AGGAAACTCT
    GCATGTTCCC CTGATAACAT GTCAAGTGCT AGTGGGTTAG ACATGCTAAA GATAGAGGAA
    ATGGAAAGAA TGCTTCTAGA AGCTCATGCA GAGCGTGCCA GGCTTGTAGA ATCCAGGGAG
    CGTGAGGTGG AGGCACGCAG AAAAGCACTA GAAGATGAGA GGCGAAGGAG AGAGGAGCTG
    GAAAGGCGTC TTCAAGATGA GACAGCTCAC AGGCAAAGAC TAGTAGAGAA GGAGGTCAAG
    ATGAGGGAGA AGCATTTTTC ACAGGCTCGT CCTCTTACAC GCTATCTGCC AATCCGGAAG
    GAAGACTTTG ATCTCCGAGC CCATGTTGAG TCCTCAGGCC ATGCAGTAGA TGCCTGCCCT
    CACATCATTT TAAGTGAGAA GATGTGTCGT GGCTTCCTGA CAAAAATGGG TGGAAAGATA
    AAATCATGGA AGAAGCGCTG GTTTGTTTTT GATCGCCTGA AACGGACCCT GTCCTATTAT
    GTAGATAAAC ATGAGGCAAA GCTGAAAGGA GTTATATATT TCCAGGCCAT AGAAGAGGTT
    TATTATGACC ACCTCAGGAG TGCAGCCAAG AGTCCTAACC CAGCTCTAAC ATTCTGTGTC
    AAGACTCACG ATCGCCTGTA TTACATGGTA GCTCCTTCCC CAGAGGCTAT GCGGATATGG
    ATGGATGTCA TTGTAACTGG GGCAGAGGGT TACACTCAGT TCATGAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821625.1
    CDS62..4552
    Translation

    Target ORF information:

    RefSeq Version XM_012966171.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966171.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA
    GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG
    CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT
    CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA
    GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA
    ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT
    GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA
    ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA
    GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA
    TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA
    GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT
    CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC
    TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA
    CAAAAAGACA GTCTGAGCAC AGAAGATTCT CTTCCATCTG AAGTTGAAGG TCAACAAGTG
    ATAGAGGAGC AGCGCAGACG TCTTGCAGAG TTGAAACAGA GAGCTGCAGT TGAGGCACAA
    TCTCAGTGGG AATCTCTCCA TGGAGCCCCT AATCAAGGGT ACACGCCTAT AATTCACCAC
    TCCATTCTAC ACCATCAGCG GCCTCTCCAT TCTCGTGAAG ATGACCCTAC CCATGGATTT
    GACACTCTCA GTCTGGAGAG TTCTGATAGT TTGGACACTA GTGTTTCTAC AGGAAACTCT
    GCATGTTCCC CTGATAACAT GTCAAGTGCT AGTGGGTTAG ACATGCTAAA GATAGAGGAA
    ATGGAAAGAA TGCTTCTAGA AGCTCATGCA GAGCGTGCCA GGCTTGTAGA ATCCAGGGAG
    CGTGAGGTGG AGGCACGCAG AAAAGCACTA GAAGATGAGA GGCGAAGGAG AGAGGAGCTG
    GAAAGGCGTC TTCAAGATGA GACAGCTCAC AGGCAAAGAC TAGTAGAGAA GGAGGTCAAG
    ATGAGGGAGA AGCATTTTTC ACAGGCTCGT CCTCTTACAC GCTATCTGCC AATCCGGAAG
    GAAGACTTTG ATCTCCGAGC CCATGTTGAG TCCTCAGGCC ATGCAGTAGA TGCCTGCCCT
    CACATCATTT TAAGTGAGAA GATGTGTCGT GGCTTCCTGA CAAAAATGGG TGGAAAGATA
    AAATCATGGA AGAAGCGCTG GTTTGTTTTT GATCGCCTGA AACGGACCCT GTCCTATTAT
    GTAGATAAAC ATGAGGCAAA GCTGAAAGGA GTTATATATT TCCAGGCCAT AGAAGAGGTT
    TATTATGACC ACCTCAGGAG TGCAGCCAAG AGTCCTAACC CAGCTCTAAC ATTCTGTGTC
    AAGACTCACG ATCGCCTGTA TTACATGGTA GCTCCTTCCC CAGAGGCTAT GCGGATATGG
    ATGGATGTCA TTGTAACTGG GGCAGAGGGT TACACTCAGT TCATGAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32410
    Clone ID Related Accession (Same CDS sequence) XM_012966172.3 , XM_012966172.2
    Accession Version XM_012966172.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4458bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pleckstrin homology-like domain family B member 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966172.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA
    GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG
    CAAGAGTACA TCACTTTAGA TCAGCTGCAG GATATTCTGG AAAGCATGCC CACAGGTTCT
    GCTCCAACTG CTGTGGCAAT GGCTGGCACT GATATCAACT CTGCCTCTTG TGCTCCTTCA
    CCTGACGACC CTCCTCCTGT ACATCTCTCG TCTTCATCTT CTTCCTCTCC CTCCATTTCT
    CTGGAGGTGA ATTCCCCAAC AACAGAAGGT CACTCTGCCC CCCTAGCACC TGATTTGGAT
    GCCTATTACC AAGATTTAGT AACTGCACCT TCTGCCTCTT CTTCTCCATC TTCTTCCCCT
    CCTCCACTCC CTGCTAAATC CCACTCATCA CGTAAGGCTT TGCAGATTTC TCGCTCTCGG
    ACAGAAATTG AGGGTGGAGG CACTCTTCCA CGGATGAAAA CAGTCTCCCC CGCTTCTTCT
    CACTTTTCAG TGGCCACTCT GGGCCGTAGT GTTTCCCCAA AGAATGCCCA TCTGACTCAG
    AATGGCACAG GCAGCCTCTC CAGGGGATTG GCAGTAACCA TGCAAGACAT TCAAAGCAAG
    AGACAGCTGG CACTGCAACA AAAAGACAGT CTGAGCACAG AAGATTCTCT TCCATCTGAA
    GTTGAAGGTC AACAAGTGAT AGAGGAGCAG CGCAGACGTC TTGCAGAGTT GAAACAGAGA
    GCTGCAGTTG AGGCACAATC TCAGTGGGAA TCTCTCCATG GAGCCCCTAA TCAAGGGTAC
    ACGCCTATAA TTCACCACTC CATTCTACAC CATCAGCGGC CTCTCCATTC TCGTGAAGAT
    GACCCTACCC ATGGATTTGA CACTCTCAGT CTGGAGAGTT CTGATAGTTT GGACACTAGT
    GTTTCTACAG GAAACTCTGC ATGTTCCCCT GATAACATGT CAAGTGCTAG TGGGTTAGAC
    ATGCTAAAGA TAGAGGAAAT GGAAAGAATG CTTCTAGAAG CTCATGCAGA GCGTGCCAGG
    CTTGTAGAAT CCAGGGAGCG TGAGGTGGAG GCACGCAGAA AAGCACTAGA AGATGAGAGG
    CGAAGGAGAG AGGAGCTGGA AAGGCGTCTT CAAGATGAGA CAGCTCACAG GCAAAGACTA
    GTAGAGAAGG AGGTCAAGAT GAGGGAGAAG CATTTTTCAC AGGCTCGTCC TCTTACACGC
    TATCTGCCAA TCCGGAAGGA AGACTTTGAT CTCCGAGCCC ATGTTGAGTC CTCAGGCCAT
    GCAGTAGATG CCTGCCCTCA CATCATTTTA AGTGAGAAGA TGTGTCGTGG CTTCCTGACA
    AAAATGGGTG GAAAGATAAA ATCATGGAAG AAGCGCTGGT TTGTTTTTGA TCGCCTGAAA
    CGGACCCTGT CCTATTATGT AGATAAACAT GAGGCAAAGC TGAAAGGAGT TATATATTTC
    CAGGCCATAG AAGAGGTTTA TTATGACCAC CTCAGGAGTG CAGCCAAGAA AGGACTTTTT
    AACCTCAGCC TGGCCACTCA GATATCCGAT TTTCTTTCTG TGCCCCTGTG GAAGGAGAGT
    CCTAACCCAG CTCTAACATT CTGTGTCAAG ACTCACGATC GCCTGTATTA CATGGTAGCT
    CCTTCCCCAG AGGCTATGCG GATATGGATG GATGTCATTG TAACTGGGGC AGAGGGTTAC
    ACTCAGTTCA TGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821626.1
    CDS63..4520
    Translation

    Target ORF information:

    RefSeq Version XM_012966172.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966172.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA
    GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG
    CAAGAGTACA TCACTTTAGA TCAGCTGCAG GATATTCTGG AAAGCATGCC CACAGGTTCT
    GCTCCAACTG CTGTGGCAAT GGCTGGCACT GATATCAACT CTGCCTCTTG TGCTCCTTCA
    CCTGACGACC CTCCTCCTGT ACATCTCTCG TCTTCATCTT CTTCCTCTCC CTCCATTTCT
    CTGGAGGTGA ATTCCCCAAC AACAGAAGGT CACTCTGCCC CCCTAGCACC TGATTTGGAT
    GCCTATTACC AAGATTTAGT AACTGCACCT TCTGCCTCTT CTTCTCCATC TTCTTCCCCT
    CCTCCACTCC CTGCTAAATC CCACTCATCA CGTAAGGCTT TGCAGATTTC TCGCTCTCGG
    ACAGAAATTG AGGGTGGAGG CACTCTTCCA CGGATGAAAA CAGTCTCCCC CGCTTCTTCT
    CACTTTTCAG TGGCCACTCT GGGCCGTAGT GTTTCCCCAA AGAATGCCCA TCTGACTCAG
    AATGGCACAG GCAGCCTCTC CAGGGGATTG GCAGTAACCA TGCAAGACAT TCAAAGCAAG
    AGACAGCTGG CACTGCAACA AAAAGACAGT CTGAGCACAG AAGATTCTCT TCCATCTGAA
    GTTGAAGGTC AACAAGTGAT AGAGGAGCAG CGCAGACGTC TTGCAGAGTT GAAACAGAGA
    GCTGCAGTTG AGGCACAATC TCAGTGGGAA TCTCTCCATG GAGCCCCTAA TCAAGGGTAC
    ACGCCTATAA TTCACCACTC CATTCTACAC CATCAGCGGC CTCTCCATTC TCGTGAAGAT
    GACCCTACCC ATGGATTTGA CACTCTCAGT CTGGAGAGTT CTGATAGTTT GGACACTAGT
    GTTTCTACAG GAAACTCTGC ATGTTCCCCT GATAACATGT CAAGTGCTAG TGGGTTAGAC
    ATGCTAAAGA TAGAGGAAAT GGAAAGAATG CTTCTAGAAG CTCATGCAGA GCGTGCCAGG
    CTTGTAGAAT CCAGGGAGCG TGAGGTGGAG GCACGCAGAA AAGCACTAGA AGATGAGAGG
    CGAAGGAGAG AGGAGCTGGA AAGGCGTCTT CAAGATGAGA CAGCTCACAG GCAAAGACTA
    GTAGAGAAGG AGGTCAAGAT GAGGGAGAAG CATTTTTCAC AGGCTCGTCC TCTTACACGC
    TATCTGCCAA TCCGGAAGGA AGACTTTGAT CTCCGAGCCC ATGTTGAGTC CTCAGGCCAT
    GCAGTAGATG CCTGCCCTCA CATCATTTTA AGTGAGAAGA TGTGTCGTGG CTTCCTGACA
    AAAATGGGTG GAAAGATAAA ATCATGGAAG AAGCGCTGGT TTGTTTTTGA TCGCCTGAAA
    CGGACCCTGT CCTATTATGT AGATAAACAT GAGGCAAAGC TGAAAGGAGT TATATATTTC
    CAGGCCATAG AAGAGGTTTA TTATGACCAC CTCAGGAGTG CAGCCAAGAA AGGACTTTTT
    AACCTCAGCC TGGCCACTCA GATATCCGAT TTTCTTTCTG TGCCCCTGTG GAAGGAGAGT
    CCTAACCCAG CTCTAACATT CTGTGTCAAG ACTCACGATC GCCTGTATTA CATGGTAGCT
    CCTTCCCCAG AGGCTATGCG GATATGGATG GATGTCATTG TAACTGGGGC AGAGGGTTAC
    ACTCAGTTCA TGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32411
    Clone ID Related Accession (Same CDS sequence) XM_012966173.3 , XM_012966173.2
    Accession Version XM_012966173.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4449bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pleckstrin homology-like domain family B member 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966173.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA
    GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG
    CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT
    CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA
    GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA
    ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT
    GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA
    ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA
    GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA
    TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA
    GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT
    CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC
    TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA
    CAAAAAGGTC AACAAGTGAT AGAGGAGCAG CGCAGACGTC TTGCAGAGTT GAAACAGAGA
    GCTGCAGTTG AGGCACAATC TCAGTGGGAA TCTCTCCATG GAGCCCCTAA TCAAGGGTAC
    ACGCCTATAA TTCACCACTC CATTCTACAC CATCAGCGGC CTCTCCATTC TCGTGAAGAT
    GACCCTACCC ATGGATTTGA CACTCTCAGT CTGGAGAGTT CTGATAGTTT GGACACTAGT
    GTTTCTACAG GAAACTCTGC ATGTTCCCCT GATAACATGT CAAGTGCTAG TGGGTTAGAC
    ATGCTAAAGA TAGAGGAAAT GGAAAGAATG CTTCTAGAAG CTCATGCAGA GCGTGCCAGG
    CTTGTAGAAT CCAGGGAGCG TGAGGTGGAG GCACGCAGAA AAGCACTAGA AGATGAGAGG
    CGAAGGAGAG AGGAGCTGGA AAGGCGTCTT CAAGATGAGA CAGCTCACAG GCAAAGACTA
    GTAGAGAAGG AGGTCAAGAT GAGGGAGAAG CATTTTTCAC AGGCTCGTCC TCTTACACGC
    TATCTGCCAA TCCGGAAGGA AGACTTTGAT CTCCGAGCCC ATGTTGAGTC CTCAGGCCAT
    GCAGTAGATG CCTGCCCTCA CATCATTTTA AGTGAGAAGA TGTGTCGTGG CTTCCTGACA
    AAAATGGGTG GAAAGATAAA ATCATGGAAG AAGCGCTGGT TTGTTTTTGA TCGCCTGAAA
    CGGACCCTGT CCTATTATGT AGATAAACAT GAGGCAAAGC TGAAAGGAGT TATATATTTC
    CAGGCCATAG AAGAGGTTTA TTATGACCAC CTCAGGAGTG CAGCCAAGAG TCCTAACCCA
    GCTCTAACAT TCTGTGTCAA GACTCACGAT CGCCTGTATT ACATGGTAGC TCCTTCCCCA
    GAGGCTATGC GGATATGGAT GGATGTCATT GTAACTGGGG CAGAGGGTTA CACTCAGTTC
    ATGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821627.1
    CDS61..4509
    Translation

    Target ORF information:

    RefSeq Version XM_012966173.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pleckstrin homology-like domain, family B, member 1 (phldb1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966173.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAAAGGT GCTGGGGTCC CCTAACTGAG TTTTACCACA GAATTGCCTG CACTTGCTGG 
    CATCAGGGGA GGGGTGGACA GGATTATTTG GAAACAGAAT TCCCTGCTCA GACAGAAATA
    GAGGACACAA TGAAGGCACT GAGTCATGAT GTGAGCAGCC CCTGCAGAAA AACACACACA
    GTTATTCAGA GCACACCATT GGATCTCATA GATACAGGAA AAAGTCTTAG GGTGCAGACA
    GAAAAACCTC ATCTTGTCAG CCTGGGAAGT GGACGACTCA GCACTGCAAT CACCCTACTA
    CCACTGGAAG AAGGAAGGAC AACCCTGGGT CATGCATCTA AGGACATTCC CCTGCAGGGA
    CCTGGCATTG CTGCAGAACA TTGCTACATT GAAAACATCA GAGGAAACCT CACCCTATAC
    CCATGTGGAA ATCCTTGTGC CATCGATGGA CTGCTCATCA CCCAACCGAC ACGTCTCACT
    CAAGGGTGTA TGGTCTGTCT GGGTCAGTCC ACCTTCCTGA GATTTAACCA CCCAGCTGAA
    GCTCACTGGA TGAAAACCAT GATTCCTTCC ACTGGGAGAA GCCCTGCGCC AGGCTTGTAC
    CCATCAGATT CTTCTTCCTC CTTGCTAAAT GGTTCTCGTG ATGCTGCATA CCCGAGAACT
    GTACCTTCCC ATACCTCACT AGTAAGCTCC ATTGAAAAAA ATCTTCAGGA CATCATGGAC
    TCGTTGGTTT TAGAAGGGCC TGCACCACTG AGCAGGAAGC TTTCCCAGAC CTCACTTAAC
    CATATCACCC CTATGACCAA TGGAGAGAAC AGACGGTACT TGCTTTCATC TCCAACAAGT
    CCACCTTACT CAAACTCAGG TTATGACAAT ACCTCTCCAC TGTCTTCTCC TGGAAGTGGA
    AGTCTCACTA GCCCCTCTCC GGGTCAAGAT GCAGGAACGC CAACAGCACC CCCTGTGGTG
    CCACTTCGAA CTTCTAGCTT TAGCCATTCC TTGCCACGAC CAGTTCCTAC CTATGGAGGA
    AGCAACCTTG ACTTGCGTTC TAATCGCAAC ACAGGTGGTG GGTCTCTGCT TAGTCCTTCA
    AGCCCAGCAA CAGGAAGCAG GATGGGGGGA CTAAGTGTGC CAAGCAGTCC ACTCCTAGGT
    AGTAAGTTTC ATCACCAGAT CACAGACCGC CCAACCAGCC CTTTTAGACA GGTGGTAGGA
    CCAACACGGA GTCTGCCACC AGAGAGTCCA AGACTTGGGC GGAGGAACTT TGAAAGCATG
    AGGGACCTGC CTCCACTTAG CCCTTCTCTG CCCCGAAGAA GTTCATCTAG CTCCATTAAA
    CCTGCTCCAG ACAGCCCATC TCGAGTTCCA GACAGCCCCA GCACTTTAGG GCGACGTCGG
    GTGGGAAGTG CTAGCTCTCC TGGCACAGAA AATAATGCAA GTACAAGGCA GATAAGGGAG
    CGCAGCCCAT CTCCCTCCTC TCTTCAAGAG ACCTCTCGTC GACTAACTCC AGCTTATAGC
    CTGGGATCGC TTGGGACCCC TTCACAAAGC CCCAGGGCAC AAAGAAAAGG GCCTGTGCTA
    CGGGAGCGCA AGAACAGCAT CAGTGAAATT TCTGGCAACC AAGAGGAGCT AATGGACTAC
    CACAGAAGGC AGAGAGAAGA AAGACTTAGA GAGCAGGAAA TGGAAAGGCT GGAGCGGCAG
    AGACTGGAAA CCATTTTAAA CTTGTGTGCC GAATACAGTA AAACAGATGA TACAGCTCCC
    CCAGCTCGCC TAGATGAAAT AAGGAGCTTC CCCACTTTGG GGGGTACTGA GGAAACCACT
    GCATCCCTCT TCACTCGTAG ACCTTCTTGG GAGAGCACCA GAAGTACCAA CCTGGAGGGA
    ACAGGCAATA GGGTGAGAGC ACAGGGAGAA CACAATGAGG AAGAGACTCA GAGAGAAGAG
    AACAGCAGTA CAGAGAGTAC CCAACATGAG CAGGAAGATG CTGTAAGCCC AACTGGGGCT
    GCAGAATTAC TAGCCCTGGA GGAGCAACGC AGGCACACCC TGGGGCATGT GGATCAGCTA
    AAGAATAGAG TGACTGAATT GGAGGGTCAG TTGCAGGAAA GTGCAAGGGA GGCAGAGATG
    GAACGTGCCC TTTTGCAAGG GGAACGGGAG GCTGAGCTGT CCCAGTTGCA GCATGAGCAA
    AGAATGGTCC AGCAGCTGCA GGACAGCCTG ACCTCTTTAG AAAACAACTT GCAGAAGGAG
    AAAGACAAGG CACGTGAGAA ACTGGAGCAG GAGAGGATGG AGCTGCAGAG TATTCAGCTT
    TCTCACCACG ACCTGCAGAG GCAGCTCCAT AACTGCCCTG AGGCTCTGCG GGAGCAGGTG
    CAGTGCCAGC TGACACGGGA GTCTGAACTC CTGGAGACGA AGATGAAGCT GTTTGAGGAT
    CTGGAGTTCC AGCAGCTAGA AAAGGAAAGC CGCCTGGAAG AAGAAAGAGA GACCACAAGT
    CAACAACTTT TACAGAGCAA GGGGGAATGC CAGCGAAGCA TTGCACGCAG GAAGGAACGC
    CTGGCTGCTT TGGAGATGCA AGCCAATCAG ATTCGCCTCC AGGCAGCACA GGATGCAGAC
    CATCTTGGCC AGGAAAGAAG CGCCATACTT TTACAGCTAC ACAAGGAGAA AGAGAAGCTG
    GTTCAGTTGG AGAGAAGATA CCAGGCACTA ACAGGACGAC GGGGATTCTC AACTGGATCA
    GCTGCTCTCC GAGAGGATGC TCTTCAAATC TATGAGACTA GAGACTGCTT ACTCGCTCAA
    GCTGATGTTT CTTGCACACC TGCTATCCCT TCTGCCTGCT CTCACTCCCC TTCCCGGCAG
    CAAGAGGAAT ACCTGTGCCT CTCTGATGTT CTCCGCTTGT ACAGCAGCTC GCCTCTTGTT
    CCTGCTTTTC CCCTTCTCTC TGCTGCAGAG CCCACCGCCG CCCTTGAGTA CATCACTTTA
    GATCAGCTGC AGGATATTCT GGAAAGCATG CCCACAGGTT CTGCTCCAAC TGCTGTGGCA
    ATGGCTGGCA CTGATATCAA CTCTGCCTCT TGTGCTCCTT CACCTGACGA CCCTCCTCCT
    GTACATCTCT CGTCTTCATC TTCTTCCTCT CCCTCCATTT CTCTGGAGGT GAATTCCCCA
    ACAACAGAAG GTCACTCTGC CCCCCTAGCA CCTGATTTGG ATGCCTATTA CCAAGATTTA
    GTAACTGCAC CTTCTGCCTC TTCTTCTCCA TCTTCTTCCC CTCCTCCACT CCCTGCTAAA
    TCCCACTCAT CACGTAAGGC TTTGCAGATT TCTCGCTCTC GGACAGAAAT TGAGGGTGGA
    GGCACTCTTC CACGGATGAA AACAGTCTCC CCCGCTTCTT CTCACTTTTC AGTGGCCACT
    CTGGGCCGTA GTGTTTCCCC AAAGAATGCC CATCTGACTC AGAATGGCAC AGGCAGCCTC
    TCCAGGGGAT TGGCAGTAAC CATGCAAGAC ATTCAAAGCA AGAGACAGCT GGCACTGCAA
    CAAAAAGGTC AACAAGTGAT AGAGGAGCAG CGCAGACGTC TTGCAGAGTT GAAACAGAGA
    GCTGCAGTTG AGGCACAATC TCAGTGGGAA TCTCTCCATG GAGCCCCTAA TCAAGGGTAC
    ACGCCTATAA TTCACCACTC CATTCTACAC CATCAGCGGC CTCTCCATTC TCGTGAAGAT
    GACCCTACCC ATGGATTTGA CACTCTCAGT CTGGAGAGTT CTGATAGTTT GGACACTAGT
    GTTTCTACAG GAAACTCTGC ATGTTCCCCT GATAACATGT CAAGTGCTAG TGGGTTAGAC
    ATGCTAAAGA TAGAGGAAAT GGAAAGAATG CTTCTAGAAG CTCATGCAGA GCGTGCCAGG
    CTTGTAGAAT CCAGGGAGCG TGAGGTGGAG GCACGCAGAA AAGCACTAGA AGATGAGAGG
    CGAAGGAGAG AGGAGCTGGA AAGGCGTCTT CAAGATGAGA CAGCTCACAG GCAAAGACTA
    GTAGAGAAGG AGGTCAAGAT GAGGGAGAAG CATTTTTCAC AGGCTCGTCC TCTTACACGC
    TATCTGCCAA TCCGGAAGGA AGACTTTGAT CTCCGAGCCC ATGTTGAGTC CTCAGGCCAT
    GCAGTAGATG CCTGCCCTCA CATCATTTTA AGTGAGAAGA TGTGTCGTGG CTTCCTGACA
    AAAATGGGTG GAAAGATAAA ATCATGGAAG AAGCGCTGGT TTGTTTTTGA TCGCCTGAAA
    CGGACCCTGT CCTATTATGT AGATAAACAT GAGGCAAAGC TGAAAGGAGT TATATATTTC
    CAGGCCATAG AAGAGGTTTA TTATGACCAC CTCAGGAGTG CAGCCAAGAG TCCTAACCCA
    GCTCTAACAT TCTGTGTCAA GACTCACGAT CGCCTGTATT ACATGGTAGC TCCTTCCCCA
    GAGGCTATGC GGATATGGAT GGATGTCATT GTAACTGGGG CAGAGGGTTA CACTCAGTTC
    ATGAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.