brf1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

  • Gene

  • Clones

  • gRNAs

The following brf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the brf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa20287 NM_001195621.1
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Xenopus tropicalis BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit (brf1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa20287
    Clone ID Related Accession (Same CDS sequence) NM_001195621.1
    Accession Version NM_001195621.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1998bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-06
    Organism Xenopus tropicalis(tropical clawed frog)
    Product transcription factor IIIB 90 kDa subunit
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAMC03032326.1, AAMC03032325.1 and AAMC03032324.1. On Sep 22, 2010 this sequence version replaced XM_002938794.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1696415, SAMEA1696417 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGAGCAGCC GGGTGTGTAA GAACTGCGGT TGTACCGATA TAGACGTAGA TGCGGCCCGC 
    GGGGATGCGG TGTGTACGGG CTGCGGCTCG GTGCTGGAGG ATAATATCAT CGTGTCTGAG
    GTGCAGTTCG TGGAGGGCGC AGGCGGGATC TCCTCGGCAG TTGGACAGTT CGTCTCCTCG
    GAATGTTCTG GTAAAACTCC ATCACTGGGA GAAGGATTTT ACACCAGCGT GGGAAAGGAG
    TCCCGTGCAC AAACTCTACA GAATGGGAAG CGTCAGATTC ACAACTTGGG AGTGCAGTTA
    CAGCTAAATA AGCATTGCCT GGACACTGCC TTCAACTTCT TTAAAATGGC TGTCAGCAAG
    CACCTTACCC GTGGTAGGAA GATGACCCAT GTCATAGCCG CCTGCTTGTA CTTGGTCTGT
    AGGACAGAAG GAACTCCCCA TATGTTACTT GATCTCAGTG ACCTTCTTCA GGTGAATGTG
    TATGTCCTTG GCAAGACCTT TCTTCTCTTA GCCAGAGAAC TCTGCATCAA TGCTCCTGCT
    ATAGACCCCT GCTTATATAT TCCTCGATTT GCACACTTGC TGGAGTTTGG CGATAAAAAT
    CATGAAGTAT CAATGACTGC ACTGAGGTTG GTCCAAAGGA TGAAAAGAGA TTGGATGCAC
    ACTGGGCGCC GCCCATCTGG ACTCTGCGGA GCTGCACTGC TTGTTGCATC CAGAATGCAT
    GATTTCCGGC GGACCATTAA AGAGGTCATC AGGGTGGTAA AGGTGTGTGA GTCTACACTG
    AGGAAAAGAT TAACAGAATT TGAAGATACT CCAACAAGTC AACTGACAAT TGATGAATTT
    ATGAAGATTG ATTTGGAACA AGAGTGTGAT CCTCCTTCCT TTACTGCTGG TCAAAGGAAG
    GTTAAAATAC AACAGCTTGA GCAAGAGTTG GCAAAAAAAC TTGATGAAGT AGAAGGGGAA
    ATATCAAGTT ATCAAGATGA AATAGAGAAT GAGCTGGAGA GTAGCAGACC AAAGCCCAAA
    GGAATATATG CTTCATATGC TAAAAATGGT TCAGAAGACA ACACTTCTAG CATACTTGCA
    GAAGAAGATG ATGATGAAGA CCTGGAAGCA GCCGCTAATC ACTTAAACAA GGATTTTTAC
    AGTGAACTTG CTAATGATAC ATCTAAAGGG ATTTGTTATG AGCACACCGT GAAGCCACTT
    CCTATAGAAT CTCTACTAGG CCCTCTCCCC ACTGCTGCAA GCTTGGGGAT TACAGACTCC
    ATCAAGGAAT GCATAGCAAT AAATGAGCAA GAGAAAAGTG ACAATGCAGG AGACGGCGAA
    CTCGACCTTA ATGGAATTGA TGACAATGAA ATAGACAGGT ATATTCTCAA TGAGAAGGAA
    GCAGAAATAA AGAAAGAGAT GTGGATGAAA GAGAATGAGG ACTACCTTCG AGAACAAAAG
    GAAAAGGAAG CGCGTATAGC TAAAGAGAAG GAGCTTGGTA TTTACAAAGA GCAAAAGCCA
    AGGAAACCTT CCAAGCGGAG GGCTCCAATC CAAGCCAGCA CTGCTGGGGA GGCAATTGAA
    AAGATGCTGG AGCAGAAGAA AATCTCCAGT AAAATTAACT ATGATGTACT CCGGGACCTC
    AACAGCAAAG GGAGCAGTAC TCCAAAGCAC GATAAGAACG ATATGGAGGA AGGGATCACT
    AGCAACCAAC TCTCCCGCAG GAAATCTGCT GCCAGTAAAA TTCTGTCCAA TCCCGTCAGC
    AACGCCGGCA AAAGGATAAG ACCACTGGTT TCAACGCAGC CATCTAAGAA AGTAGCATTT
    GAACAGGAAG TCAGAAATGA ACTTGAGTCA ACACCCCCAA AGATAGTAGT GGAGAGTGGG
    CCAGTCACAT ACAATCACGA GGATGATGCC GCCGCCGCCG AAGAAGAAGA GGAGGAAGAG
    GAAGAACACT GTGTGAGCGC ACTTCAGTTA ATGGGGGGAA ATGATTACGG CTGTGATATG
    GATGAGGATG ATTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001182550.1
    CDS139..2136
    Translation

    Target ORF information:

    RefSeq Version NM_001195621.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit (brf1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001195621.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGAGCAGCC GGGTGTGTAA GAACTGCGGT TGTACCGATA TAGACGTAGA TGCGGCCCGC 
    GGGGATGCGG TGTGTACGGG CTGCGGCTCG GTGCTGGAGG ATAATATCAT CGTGTCTGAG
    GTGCAGTTCG TGGAGGGCGC AGGCGGGATC TCCTCGGCAG TTGGACAGTT CGTCTCCTCG
    GAATGTTCTG GTAAAACTCC ATCACTGGGA GAAGGATTTT ACACCAGCGT GGGAAAGGAG
    TCCCGTGCAC AAACTCTACA GAATGGGAAG CGTCAGATTC ACAACTTGGG AGTGCAGTTA
    CAGCTAAATA AGCATTGCCT GGACACTGCC TTCAACTTCT TTAAAATGGC TGTCAGCAAG
    CACCTTACCC GTGGTAGGAA GATGACCCAT GTCATAGCCG CCTGCTTGTA CTTGGTCTGT
    AGGACAGAAG GAACTCCCCA TATGTTACTT GATCTCAGTG ACCTTCTTCA GGTGAATGTG
    TATGTCCTTG GCAAGACCTT TCTTCTCTTA GCCAGAGAAC TCTGCATCAA TGCTCCTGCT
    ATAGACCCCT GCTTATATAT TCCTCGATTT GCACACTTGC TGGAGTTTGG CGATAAAAAT
    CATGAAGTAT CAATGACTGC ACTGAGGTTG GTCCAAAGGA TGAAAAGAGA TTGGATGCAC
    ACTGGGCGCC GCCCATCTGG ACTCTGCGGA GCTGCACTGC TTGTTGCATC CAGAATGCAT
    GATTTCCGGC GGACCATTAA AGAGGTCATC AGGGTGGTAA AGGTGTGTGA GTCTACACTG
    AGGAAAAGAT TAACAGAATT TGAAGATACT CCAACAAGTC AACTGACAAT TGATGAATTT
    ATGAAGATTG ATTTGGAACA AGAGTGTGAT CCTCCTTCCT TTACTGCTGG TCAAAGGAAG
    GTTAAAATAC AACAGCTTGA GCAAGAGTTG GCAAAAAAAC TTGATGAAGT AGAAGGGGAA
    ATATCAAGTT ATCAAGATGA AATAGAGAAT GAGCTGGAGA GTAGCAGACC AAAGCCCAAA
    GGAATATATG CTTCATATGC TAAAAATGGT TCAGAAGACA ACACTTCTAG CATACTTGCA
    GAAGAAGATG ATGATGAAGA CCTGGAAGCA GCCGCTAATC ACTTAAACAA GGATTTTTAC
    AGTGAACTTG CTAATGATAC ATCTAAAGGG ATTTGTTATG AGCACACCGT GAAGCCACTT
    CCTATAGAAT CTCTACTAGG CCCTCTCCCC ACTGCTGCAA GCTTGGGGAT TACAGACTCC
    ATCAAGGAAT GCATAGCAAT AAATGAGCAA GAGAAAAGTG ACAATGCAGG AGACGGCGAA
    CTCGACCTTA ATGGAATTGA TGACAATGAA ATAGACAGGT ATATTCTCAA TGAGAAGGAA
    GCAGAAATAA AGAAAGAGAT GTGGATGAAA GAGAATGAGG ACTACCTTCG AGAACAAAAG
    GAAAAGGAAG CGCGTATAGC TAAAGAGAAG GAGCTTGGTA TTTACAAAGA GCAAAAGCCA
    AGGAAACCTT CCAAGCGGAG GGCTCCAATC CAAGCCAGCA CTGCTGGGGA GGCAATTGAA
    AAGATGCTGG AGCAGAAGAA AATCTCCAGT AAAATTAACT ATGATGTACT CCGGGACCTC
    AACAGCAAAG GGAGCAGTAC TCCAAAGCAC GATAAGAACG ATATGGAGGA AGGGATCACT
    AGCAACCAAC TCTCCCGCAG GAAATCTGCT GCCAGTAAAA TTCTGTCCAA TCCCGTCAGC
    AACGCCGGCA AAAGGATAAG ACCACTGGTT TCAACGCAGC CATCTAAGAA AGTAGCATTT
    GAACAGGAAG TCAGAAATGA ACTTGAGTCA ACACCCCCAA AGATAGTAGT GGAGAGTGGG
    CCAGTCACAT ACAATCACGA GGATGATGCC GCCGCCGCCG AAGAAGAAGA GGAGGAAGAG
    GAAGAACACT GTGTGAGCGC ACTTCAGTTA ATGGGGGGAA ATGATTACGG CTGTGATATG
    GATGAGGATG ATTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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