hectd1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

  • Gene

  • Clones

  • gRNAs

The following hectd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hectd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa63529 XM_012968096.3
Latest version!
Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 1 (hectd1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa63529 XM_012968095.3
Latest version!
Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 1 (hectd1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa63530 XM_012968097.3
Latest version!
Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 1 (hectd1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa22713 NM_001137613.1
Latest version!
Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 1 (hectd1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OXa32491 XM_012968096.2
Latest version!
Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 1 (hectd1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OXa32491 XM_012968095.2
Latest version!
Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 1 (hectd1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OXa32492 XM_012968097.2
Latest version!
Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 1 (hectd1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa63529
    Clone ID Related Accession (Same CDS sequence) XM_012968095.3 , XM_012968096.3
    Accession Version XM_012968095.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012968095.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGGCTGACG TAGATCCAGA CACACTACTG GAATGGCTGC AGATGGGGCA GGGGGATGAG 
    AGAGACATGC AGCTCATTGC CTTGGAACAG CTCTGCATGC TGCTGCTCAT GTCGGATAAT
    GTGGATAGGT GTTTTGAAAC ATGCCCACCT CGCACCTTCC TGCCAGCCTT GTGTAAAATA
    TTCCTGGATG AAAGCGCCCC AGACAATGTG TTGGAAGTAA CAGCCCGTGC AATAACCTAT
    TACCTAGATG TGTCCGCCGA GTGCACGAGG CGTATTGTGG GAGTGGAAGG GGCTATCAAA
    GCTTTGTGCA ATAGGCTTGT GGTGGTGGAG CTCAACAACA GAACCAGTCG GGATTTGGCA
    GAACAGTGTG TCAAGGTGCT GGAGCTTATC TGTACACGTG AGTCTGGGGC TGTCTTTGAG
    GCAGGGGGCT TGAACTGCGT GTTGACATTC ATTCGGGACA GCGGACACCT AGTTCACAAG
    GATACACTGC ATTCTGCAAT GGCTGTGGTG TCCCGATTGT GTGGTAAGAT GGAGCCACAG
    GATGCATCAT TAGAAACGTG TGTGGAGTCT CTCTCCAGCC TGCTGAAGCA CGAAGACCAC
    CAGGTATCAG ATGGAGCTCT CCGCTGCTTT GCTTCCCTTG CTGACCGCTT TACACGCCGT
    GGTGTAGATC CAGCTCCTCT TGCACAGCAT GGGCTCACTG AGGAACTGCT GTCCCGAATG
    GCAGCAGCCG GAGGCACTGT ATCAGGTCCA TCTTCAGCCT GTAAAACAGG CCGTGGCACC
    AGTGGGGGAC CTTCCACCTC TGGAGACTCA AAAATCAGCA ATCAGGTCTC CACTATAGTC
    AGCCTGTTAT CAACACTATG CCGTGGTTCT CCAGTCGTCA CACATGATTT ACTGCGGGCA
    GAACTCTTAG ACTCCATGGA AAGTGCTCTT CAAGGTGACG AGCGTTGTGT GCTGGATACC
    ATGCGCCTTG TAGACCTTCT TTTGGTGCTG CTTTTTGAAG GCCGCAAAGC TCTGCCTAAG
    TCTAGTGCTG GGTCCACAGG TCGTATTCCT GGGCTGCGGC GGCTTGACAG CTCAGGAGAG
    CGATCTCACC GCCAACTGAT AGACTGCATC CGCAGCAAGG ACACAGATGC TCTCATAGAT
    GCCATTGATA CAGGTGCTTT TGAAGTAAAT TTTATGGATG ATGTTGGCCA GACTCTTCTG
    AATTGGGCCT CTGCTTTTGG CACACAGGAA ATGGTTGAAT TCCTGTGTGA GCGAGGTGCC
    GATGTAAATA GAGGGCAGAG GTCATCTTCA CTGCATTATG CTGCTTGCTT CGGAAGGCCT
    CAGGTGGCAA AGACCTTGCT TCGCCATGGA GCCAACCCAG ATTTAAGAGA TGAGGATGGA
    AAAACACCTT TGGATAAAGC CCGGGAGAGA GGACACAGCG AAGTGGTGGC CATTTTGCAG
    TCCCCAGGGG ACTGGATGTG CCCCGTAAAC AAAGGAGATG AAAAGAAAAA GAAAGACTCT
    AACCGGGAAG AGGAAGAATG CAATGAGCCA AAGGGAGACC CAGAAATGGC CCCCATCTAC
    CTAAAGAGGC TGCTGCCTGT CTTTGCACAG ACCTTTCAGC AAACCATGCT TCCCTCTATC
    AGGAAAGCAA GCTTAGCACT TATTCGGAAG ATGATCCATT TCTGCTCAGA AGCACTGCTT
    AAGGAAGTTT GTCACTCAGA CGCTGGACAC AATTTGCCCA CTGTTCTTGT TGAGATAACA
    GCCACTGTTT TAGACCAGGA GGACGATGAT GATGGTCACC TTCTGGCACT TCAAATCATT
    AGGGATTTGG TGGACAAAGG TGATGACCTT TTTCTTGATC AATTGGCCCG GCTTGGTGTT
    ATAAGCAAGG TTTCTACACT AGCTGGACCT ACATCAGATG ATGAGAATGA GGAGGATAGC
    AAGCCAGAGA AGGTGAGGAT ACACCTATTT CCTCTGTACA GTGCCCACAA AGGAACACAA
    GCAGCTGCAT CACAACTTAA AGAGGATGAG CCACAGGAAG ATGCCAAGGA GTTGCAACAG
    GGCCGGCCCT ACCATTGGCG GGACTGGTCT GTTATTCGCG GTAGGGACTG TTTGTACATC
    TGGAGTGATG CTGCAGCTCT GGAGCTCTCT AATGGTAGCA ATGGCTGGTT CCGCTTTATC
    CTAGATGGGA AACTGGCAAC CATGTATTCA AGTGGAAGCC CAGAGGGGGG TTCTGATAGC
    TCAGAAAGTA GAAGTGAATT TTTAGAAAAG TTACAGCGGG CCCGCAGCCA GGTCAAACCA
    TCCACTTCTA GCCAGCCTAT CTTGTCCACT CCTGGTCCAG GAAAGCTAAC AGTAGGCAAC
    TGGTCTCTCA CTTGTCTGAA AGATGGTGAG ATTGCAATCC ACAACTCGGA CGGGCAGCAG
    GCTACAATAC TGAAGGAGGA TCTACCAGGC TTTGTGTTTG AGTCAAACAG GGGTACCAAG
    CACTCCTTCA CGGCAGAAAC ATCACTAGGA TCTGAATTTG TAACTGGCTG GACAGGAAAG
    AGAGGCAGAA AGCTGAAGTC TAAGTTGGAG AAAACTAAAC AGAAGGTACG GACAATGGCA
    AGAGATTTGT ATGATGATCA TTTTAAGGCA GTGGAAAGCA TGCCCCGAGG AGTGGTAGTG
    ACGCTCCGAA ATATTGCAAC ACAGCTGGAA TCTGCTTGGG AACTTCATAC AAACAGACAG
    TGTATTGAAG GAGAGAACAC ATGGAGAGAT CTGATGAAAA CTGCCCTGGA AAACCTGATT
    GTGCTACTGA AGGATGAGAA TACTATTTCC CCTTATGAGA TGTGCAGCAG TGGACTGGTG
    CAGGCTTTGC TTACGGTGCT CAATAATAAT GAAGACTGTG ATATAAAACA AGACTGTGGG
    CAGCTGGTGG AGAGACTTAA TGTATTTAAA ACTGCCTTCA GCGAAAATGA AGATGATGAA
    AGCCGCCCTG CCGTTGCCCT CGTCCGGAAG TTGATTGCTG TGCTTGAATC AATAGAACGG
    CTGCCCCTTC ACCTGTATGA CACCCCTGGA TCTAGCTATA ACTTGCAGAT CCTGACCCGT
    AGGCTTCGGT TTCGGCTGGA GCGTGCACCT GGAGAAACGT CTCTAATCGA CCGTACCGGT
    CGGATGCTGA AAATGGAGCC ACTGGCAACT GTAGAGTCCT TGGAGCAGTA TTTACTGAAA
    ATGGTGGCTA AGCAGTGGTA CGACTTTGAC CGTTCGTCCT TTGTGTTTGT GCGCAAATTG
    CGCGAGGGCC AGAGTTGTGT GTTCCGCCAC CAGCATGACT TTGATGATAA TGGAATCATG
    TACTGGATTG GCACCAATGC AAAGACAGCT TATGAGTGGG TCAACCCTGC TGCTTACGGA
    CTAGTAGTTG TTACATCTTC TGAAGGCCGC AATTTGCCAT ATGGCCGCCT TGAGGATATC
    CTGAGCCGTG ATAGCTCTGC CCTTAATTGC CACACTAATG ATGATAAGAG TGCCTGGTTT
    GCCATTGACC TTGGCTTGTG GGTTGTGCCT TCTGCCTATA CACTGCGTCA TGCTCGTGGA
    TATGGCCGTT CCGCTCTTCG CAATTGGGTC TTCCAGGTGT CTAAAGATGG TCAGAACTGG
    ACCACCCTCT ACACCCACAT GGATGACTGC AGCCTGAATG AACCTGGCTC CACAGCCACA
    TGGCCTCTGG ACCCTGCGAG AGAAGAAAAG CAGGGTTGGC GTCATGTCCG GATAAAGCAG
    ACGGGAAAGA ATGCCAGTGG CCAGACCCAC TACCTCTCAC TTTCTGGCTT TGAGCTTTAT
    GGCAATGTGA CTGGGGTGTG TGAAGACCAA CTTGGTAAAG CTGCCAAAGA GGCAGAAGCC
    AACTTAAGGC GCCAGAGGCG GCTGGTACGC TCTCAGGTGC TAAAATATAT GGTGCCTGGA
    GCCCGTGTTA TTCGGGGCAT TGATTGGAAG TGGCGAGACC AAGATGGCAG TGCTCAAGGA
    GAAGGCACTG TTACAGGAGA ACTGCACAAT GGCTGGATTG ATGTCATATG GGATGCCGGT
    GGCTCTAACT CTTACCGCAT GGGCGCGGAA GGAAAATTTG ACCTGAAGCT GGCCCCGGGT
    TATGACCCAG AGTCTGCGGT GTCACCCAAA CCTGTTTCAT CCACTGTTGC AGGAACACCG
    CCCTCGTGGA GCAGCCTGGT GAAAAACAAC TGTCCTGACA AAGCCCCTCC CTCTTCCTCA
    TCCTCTTGTG TGGTCGTTGG AAGCGTGGCG GGCTCAGGCA GCCGCAAAGG AAGCAGCAGC
    TCGGTCTGTA GCGTGGCAAG CAGCAGCGAC GTAAGCCTAA GCTGTGCAAA GACAGAGAGG
    AGGGCAGAGG AGCAAGTCTC CGACATTCAC CATGATCCCA TCCTGCTTCT GTCCTCTAAC
    CAAGCAGCCT CGGGTTCCTC CACCTGCCCC CCTGGAGGAG AGACTGTGGG TGAAGGTGGA
    GATAGAAAGG CAGGAGAAGC ACCAGCAATC TCAATGGGTA TGGTGAGCAT TAGCTCCCCT
    GATGTCAGTT CCGTTTCCGA ACTCAGCAAC AAGGAAGTGG CAGTTCCACG ACCACTGGGA
    TCTTCTGCCA GCAACAGATT GTCTGTCAGT TCACTGTTGG CAGCTGGGGC ACCCATGAGT
    TCTAGCGCCA GTGTACCAAA CTTGTCCTCC AGGGAAACGT CCAGTCTGGA GTCCTTTGTA
    AGGAGAGTGG CAAATATCGC CAGGACAAAT GCAACCAACA ACATGAACCT GAGTCGTAGC
    AGCAGTGATA ACAATACCAA CACTCTGGGG AGGAATGCAG TCAGCTCAGC CACTTCACCA
    CTTATGGGAG CTCAGAGCTT TCCCAACCTG ACGACAACAG GAACCACATC CACCGTTACA
    ATGTCTACAT CCAGTGTCAC CAGCAGTAAT GTGGCAACAG CCACTACAGG GTTGTCTGTA
    GGTCAGTCCC TCAGTAACAC CCTGACTACA AGCCTAACCT CGACATCCAG TGAGAGTGAC
    ACTGGGCAGG AGGCAGAATA CTCCCTGTAT GACTTTCTTG ATAGCTGCCG TGCCAGCACT
    TTGCTTGCTG AACTTGATGA TGACGAGGAT CTCCCTGAGC CCGATGAGGA AGATGATGAA
    AACGAAGATG ATAATCAGGA AGAGCAAGAG TATGAAGAAG TTATGGAGGA GGAAGAGTAT
    GAGACCAAAG GTGGCCGACG CAGGACCTGG GATGATGATT ATGTGCTGAA GAGGCAGTTT
    TCTGCTTTGG TCCCGGCTTT TGACCCACGC CCAGGGAGAA CTAATGTTCA GCAGACCACT
    GATCTTGAAA TTCCTGCTCC AGGCACGCCC CACTCTGAGC TACTGGAGGA GGTAGAATGT
    GCCCCAGCTC CACGATTAGC ACTCACCCTA AAGGTGACAG GCCTTGGGAG CGGGAGAGAG
    GTGGAGCTCC CCCTGAACAA TTTCCGTTCC ACCATATTTT TCTATGTACA GAGACTGCTG
    CAACTCTCCT GCAATGGAGC CATCAAAACT GACAAGCTGA GAAGGATTTG GGAGCCCACC
    TACACGATTA TGTACAGAGA GATGAAGGAT TCAGATAAGC AAAAAGAATG TGGCAGACTG
    GGCTGTTGGT CTGTAGAGCA TGTAGAACAA TCCCTAGGCA CAGATGCCTT GCCAAAGAAT
    GACCTGATCA CTTACCTGCA GCGAAATGCA GATCCAGGGT TCTTGCGACG CTGGAAACTG
    ACAGGAACCA ACAAAAGCAT TAGGAAAAAC CGCAACTGTT CCCAGCTTAT TGCTGCTTAC
    AAGGACTTCT GTGAGAATGG CTGCAAGTCT CTCTCAATGC CAGCTGCTCT TGCCACACTC
    CAGAGTGCTG ATATCCTTAG TCATTCCCGA GAGCAGGCAC AAGCCAAAGC GGGCAGCAGC
    CAAAACTCCT GCGGGGTAGA AGATGTTTTG CAGCTCCTGC GAATTCTCTT TATTGTAGCC
    AGTGACCCAT ATTCTGCCAG GACCCCACAG GAAGATGGAG AAGATATGCT CCTTTTCAGT
    GTTCCTCCAG AGGAGTTTAC TAGCAAGAAA ATCACCACAA AGATTGTTCA GCAAATCGAG
    GAACCTTTAG CTTTAGCAAG TGGAGCTTTG CCAGACTGGT GTGAACAGCT GACCAGCAAA
    TGTCCCTTCT TAATACCCTT TGAAACCCGG CAGCTTTACT TCACATGCAC AGCATTTGGT
    GCCTCAAGGG CTATTGTGTG GCTGCAGAAC AGGCGGGAAG CCACAGTTGA GAGGAGCAGA
    ACAGCAAGTG CAGTGCGCAG AGACGACCCA GGGGAGTTCA GAGTGGGCCG TCTAAAACAC
    GAACGTGTGA AGGTCCCACG AGGGGAGTCT TTGATGGAGT GGGCAGAGAA TGTTATGCAA
    ATCCACGCAG ACCGAAAATC TGTGCTTGAG GTGGAGTTTC TGGGAGAAGA AGGAACAGGC
    TTGGGCCCTA CCCTTGAGTT TTATGCCCTG GTGGCAGCAG AGTTCCAGAA AACAGATTTG
    GGGATCTGGT TATGTGATGA TGACTTCCCA GATGATGAGT CTCGGCAGGT GGATCTAGGT
    GGTGGTTTGA AGCCCCCTGG TTACTATGTG CAGCGTTCCT GTGGCCTCTT CATTGCACCC
    TACCCACAGG ATAGTGAGGA ACTAGACCGA GTGACCAGAC TTTGCCATTT CCTTGGTGTT
    TTCCTTGCAA AGTGCATCCA GGACAATAGA TTAGTGGACT TGCCCATTTC AAAGCCGTTT
    TTTAAGCTTA TGTGTATGGG TGACATTAAG AGCAACATGA GCAAGCTTTT GTATGCCTCT
    CGGGGAGAGG AGAGTGAGCA CTGCACGGAG AGCCAGTCGG AGGCCTCTAC TGAGGATGGG
    CATGATGCCC TTTCAGTAGG AAGCTTTGAG GAAGACTGCA AATCTGAGTT CATCCTAGAC
    CCACCCAAAC CAAAGCCACC AGCCTGGTTT CAGGGTATTT TAACTTGGGA GGACTTTGAG
    CTAATAAACC CACATCGTGC TCGCTTCCTT AGGGACATTA GGGAGTTGGC TGTAAAGAGG
    AGACAGATTC TGGGGAACCG TTGCTTATCT GAGGATGAGA AGAACACTCA ACTTCAGGAA
    CTTATGCTAA AGAATCCTTC TGGATCTGGG CCTCCAGTCA GCATTGAGGA TTTGGGACTC
    AATTTCCAGT TCTGTCCATC TTCCCGGGTT TATGGCTTCT CTGCAGTCGA TCTAAGGCCA
    AATGGAGAGG ATGAGATGGT GACGATAGAC AACGCTGAGG AGTATGTTGA TCTTATGTTT
    GATTTCTGCA TGCAGACTGG TGTACAAAAG CAAATGGAAG CATTTAGAAG TGGCTTTAAT
    AAAGTGTTCC CTATGGAGAA ACTGGGCTCC TTCAGCCCCG AGGAAGTCCA AATGATACTG
    TGTGGAAACC AGTCTCCCTC TTGGTCCGCT GAGGACATCA TCAATTACAC AGAACCAAAG
    CTAGGATATA CCCGAGAGAG TCCTGGGTTC CTTCGATTCG TGAGAGTCCT ATGTGGCATG
    TCGTCAGATG AGAGGAAAGC GTTCCTGCAG TTTACAACGG GGTGCTCCAC ATTGCCTCCA
    GGAGGACTGG CCAATCTGCA TCCGCGTCTC ACAGTCGTGC GCAAGGTTGA TGCCACGGAT
    GCCAGCTACC CATCTGTAAA TACATGTGTG CATTACCTGA AGCTGCCGGA GTATTCCTCC
    GAGGAAATCA TGAGAGACCG CCTGCTTGCT GCTACCATGG AGAAAGGATT CCATCTCAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012823549.1
    CDS305..8047
    Translation

    Target ORF information:

    RefSeq Version XM_012968095.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 1 (hectd1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012968095.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGGCTGACG TAGATCCAGA CACACTACTG GAATGGCTGC AGATGGGGCA GGGGGATGAG 
    AGAGACATGC AGCTCATTGC CTTGGAACAG CTCTGCATGC TGCTGCTCAT GTCGGATAAT
    GTGGATAGGT GTTTTGAAAC ATGCCCACCT CGCACCTTCC TGCCAGCCTT GTGTAAAATA
    TTCCTGGATG AAAGCGCCCC AGACAATGTG TTGGAAGTAA CAGCCCGTGC AATAACCTAT
    TACCTAGATG TGTCCGCCGA GTGCACGAGG CGTATTGTGG GAGTGGAAGG GGCTATCAAA
    GCTTTGTGCA ATAGGCTTGT GGTGGTGGAG CTCAACAACA GAACCAGTCG GGATTTGGCA
    GAACAGTGTG TCAAGGTGCT GGAGCTTATC TGTACACGTG AGTCTGGGGC TGTCTTTGAG
    GCAGGGGGCT TGAACTGCGT GTTGACATTC ATTCGGGACA GCGGACACCT AGTTCACAAG
    GATACACTGC ATTCTGCAAT GGCTGTGGTG TCCCGATTGT GTGGTAAGAT GGAGCCACAG
    GATGCATCAT TAGAAACGTG TGTGGAGTCT CTCTCCAGCC TGCTGAAGCA CGAAGACCAC
    CAGGTATCAG ATGGAGCTCT CCGCTGCTTT GCTTCCCTTG CTGACCGCTT TACACGCCGT
    GGTGTAGATC CAGCTCCTCT TGCACAGCAT GGGCTCACTG AGGAACTGCT GTCCCGAATG
    GCAGCAGCCG GAGGCACTGT ATCAGGTCCA TCTTCAGCCT GTAAAACAGG CCGTGGCACC
    AGTGGGGGAC CTTCCACCTC TGGAGACTCA AAAATCAGCA ATCAGGTCTC CACTATAGTC
    AGCCTGTTAT CAACACTATG CCGTGGTTCT CCAGTCGTCA CACATGATTT ACTGCGGGCA
    GAACTCTTAG ACTCCATGGA AAGTGCTCTT CAAGGTGACG AGCGTTGTGT GCTGGATACC
    ATGCGCCTTG TAGACCTTCT TTTGGTGCTG CTTTTTGAAG GCCGCAAAGC TCTGCCTAAG
    TCTAGTGCTG GGTCCACAGG TCGTATTCCT GGGCTGCGGC GGCTTGACAG CTCAGGAGAG
    CGATCTCACC GCCAACTGAT AGACTGCATC CGCAGCAAGG ACACAGATGC TCTCATAGAT
    GCCATTGATA CAGGTGCTTT TGAAGTAAAT TTTATGGATG ATGTTGGCCA GACTCTTCTG
    AATTGGGCCT CTGCTTTTGG CACACAGGAA ATGGTTGAAT TCCTGTGTGA GCGAGGTGCC
    GATGTAAATA GAGGGCAGAG GTCATCTTCA CTGCATTATG CTGCTTGCTT CGGAAGGCCT
    CAGGTGGCAA AGACCTTGCT TCGCCATGGA GCCAACCCAG ATTTAAGAGA TGAGGATGGA
    AAAACACCTT TGGATAAAGC CCGGGAGAGA GGACACAGCG AAGTGGTGGC CATTTTGCAG
    TCCCCAGGGG ACTGGATGTG CCCCGTAAAC AAAGGAGATG AAAAGAAAAA GAAAGACTCT
    AACCGGGAAG AGGAAGAATG CAATGAGCCA AAGGGAGACC CAGAAATGGC CCCCATCTAC
    CTAAAGAGGC TGCTGCCTGT CTTTGCACAG ACCTTTCAGC AAACCATGCT TCCCTCTATC
    AGGAAAGCAA GCTTAGCACT TATTCGGAAG ATGATCCATT TCTGCTCAGA AGCACTGCTT
    AAGGAAGTTT GTCACTCAGA CGCTGGACAC AATTTGCCCA CTGTTCTTGT TGAGATAACA
    GCCACTGTTT TAGACCAGGA GGACGATGAT GATGGTCACC TTCTGGCACT TCAAATCATT
    AGGGATTTGG TGGACAAAGG TGATGACCTT TTTCTTGATC AATTGGCCCG GCTTGGTGTT
    ATAAGCAAGG TTTCTACACT AGCTGGACCT ACATCAGATG ATGAGAATGA GGAGGATAGC
    AAGCCAGAGA AGGTGAGGAT ACACCTATTT CCTCTGTACA GTGCCCACAA AGGAACACAA
    GCAGCTGCAT CACAACTTAA AGAGGATGAG CCACAGGAAG ATGCCAAGGA GTTGCAACAG
    GGCCGGCCCT ACCATTGGCG GGACTGGTCT GTTATTCGCG GTAGGGACTG TTTGTACATC
    TGGAGTGATG CTGCAGCTCT GGAGCTCTCT AATGGTAGCA ATGGCTGGTT CCGCTTTATC
    CTAGATGGGA AACTGGCAAC CATGTATTCA AGTGGAAGCC CAGAGGGGGG TTCTGATAGC
    TCAGAAAGTA GAAGTGAATT TTTAGAAAAG TTACAGCGGG CCCGCAGCCA GGTCAAACCA
    TCCACTTCTA GCCAGCCTAT CTTGTCCACT CCTGGTCCAG GAAAGCTAAC AGTAGGCAAC
    TGGTCTCTCA CTTGTCTGAA AGATGGTGAG ATTGCAATCC ACAACTCGGA CGGGCAGCAG
    GCTACAATAC TGAAGGAGGA TCTACCAGGC TTTGTGTTTG AGTCAAACAG GGGTACCAAG
    CACTCCTTCA CGGCAGAAAC ATCACTAGGA TCTGAATTTG TAACTGGCTG GACAGGAAAG
    AGAGGCAGAA AGCTGAAGTC TAAGTTGGAG AAAACTAAAC AGAAGGTACG GACAATGGCA
    AGAGATTTGT ATGATGATCA TTTTAAGGCA GTGGAAAGCA TGCCCCGAGG AGTGGTAGTG
    ACGCTCCGAA ATATTGCAAC ACAGCTGGAA TCTGCTTGGG AACTTCATAC AAACAGACAG
    TGTATTGAAG GAGAGAACAC ATGGAGAGAT CTGATGAAAA CTGCCCTGGA AAACCTGATT
    GTGCTACTGA AGGATGAGAA TACTATTTCC CCTTATGAGA TGTGCAGCAG TGGACTGGTG
    CAGGCTTTGC TTACGGTGCT CAATAATAAT GAAGACTGTG ATATAAAACA AGACTGTGGG
    CAGCTGGTGG AGAGACTTAA TGTATTTAAA ACTGCCTTCA GCGAAAATGA AGATGATGAA
    AGCCGCCCTG CCGTTGCCCT CGTCCGGAAG TTGATTGCTG TGCTTGAATC AATAGAACGG
    CTGCCCCTTC ACCTGTATGA CACCCCTGGA TCTAGCTATA ACTTGCAGAT CCTGACCCGT
    AGGCTTCGGT TTCGGCTGGA GCGTGCACCT GGAGAAACGT CTCTAATCGA CCGTACCGGT
    CGGATGCTGA AAATGGAGCC ACTGGCAACT GTAGAGTCCT TGGAGCAGTA TTTACTGAAA
    ATGGTGGCTA AGCAGTGGTA CGACTTTGAC CGTTCGTCCT TTGTGTTTGT GCGCAAATTG
    CGCGAGGGCC AGAGTTGTGT GTTCCGCCAC CAGCATGACT TTGATGATAA TGGAATCATG
    TACTGGATTG GCACCAATGC AAAGACAGCT TATGAGTGGG TCAACCCTGC TGCTTACGGA
    CTAGTAGTTG TTACATCTTC TGAAGGCCGC AATTTGCCAT ATGGCCGCCT TGAGGATATC
    CTGAGCCGTG ATAGCTCTGC CCTTAATTGC CACACTAATG ATGATAAGAG TGCCTGGTTT
    GCCATTGACC TTGGCTTGTG GGTTGTGCCT TCTGCCTATA CACTGCGTCA TGCTCGTGGA
    TATGGCCGTT CCGCTCTTCG CAATTGGGTC TTCCAGGTGT CTAAAGATGG TCAGAACTGG
    ACCACCCTCT ACACCCACAT GGATGACTGC AGCCTGAATG AACCTGGCTC CACAGCCACA
    TGGCCTCTGG ACCCTGCGAG AGAAGAAAAG CAGGGTTGGC GTCATGTCCG GATAAAGCAG
    ACGGGAAAGA ATGCCAGTGG CCAGACCCAC TACCTCTCAC TTTCTGGCTT TGAGCTTTAT
    GGCAATGTGA CTGGGGTGTG TGAAGACCAA CTTGGTAAAG CTGCCAAAGA GGCAGAAGCC
    AACTTAAGGC GCCAGAGGCG GCTGGTACGC TCTCAGGTGC TAAAATATAT GGTGCCTGGA
    GCCCGTGTTA TTCGGGGCAT TGATTGGAAG TGGCGAGACC AAGATGGCAG TGCTCAAGGA
    GAAGGCACTG TTACAGGAGA ACTGCACAAT GGCTGGATTG ATGTCATATG GGATGCCGGT
    GGCTCTAACT CTTACCGCAT GGGCGCGGAA GGAAAATTTG ACCTGAAGCT GGCCCCGGGT
    TATGACCCAG AGTCTGCGGT GTCACCCAAA CCTGTTTCAT CCACTGTTGC AGGAACACCG
    CCCTCGTGGA GCAGCCTGGT GAAAAACAAC TGTCCTGACA AAGCCCCTCC CTCTTCCTCA
    TCCTCTTGTG TGGTCGTTGG AAGCGTGGCG GGCTCAGGCA GCCGCAAAGG AAGCAGCAGC
    TCGGTCTGTA GCGTGGCAAG CAGCAGCGAC GTAAGCCTAA GCTGTGCAAA GACAGAGAGG
    AGGGCAGAGG AGCAAGTCTC CGACATTCAC CATGATCCCA TCCTGCTTCT GTCCTCTAAC
    CAAGCAGCCT CGGGTTCCTC CACCTGCCCC CCTGGAGGAG AGACTGTGGG TGAAGGTGGA
    GATAGAAAGG CAGGAGAAGC ACCAGCAATC TCAATGGGTA TGGTGAGCAT TAGCTCCCCT
    GATGTCAGTT CCGTTTCCGA ACTCAGCAAC AAGGAAGTGG CAGTTCCACG ACCACTGGGA
    TCTTCTGCCA GCAACAGATT GTCTGTCAGT TCACTGTTGG CAGCTGGGGC ACCCATGAGT
    TCTAGCGCCA GTGTACCAAA CTTGTCCTCC AGGGAAACGT CCAGTCTGGA GTCCTTTGTA
    AGGAGAGTGG CAAATATCGC CAGGACAAAT GCAACCAACA ACATGAACCT GAGTCGTAGC
    AGCAGTGATA ACAATACCAA CACTCTGGGG AGGAATGCAG TCAGCTCAGC CACTTCACCA
    CTTATGGGAG CTCAGAGCTT TCCCAACCTG ACGACAACAG GAACCACATC CACCGTTACA
    ATGTCTACAT CCAGTGTCAC CAGCAGTAAT GTGGCAACAG CCACTACAGG GTTGTCTGTA
    GGTCAGTCCC TCAGTAACAC CCTGACTACA AGCCTAACCT CGACATCCAG TGAGAGTGAC
    ACTGGGCAGG AGGCAGAATA CTCCCTGTAT GACTTTCTTG ATAGCTGCCG TGCCAGCACT
    TTGCTTGCTG AACTTGATGA TGACGAGGAT CTCCCTGAGC CCGATGAGGA AGATGATGAA
    AACGAAGATG ATAATCAGGA AGAGCAAGAG TATGAAGAAG TTATGGAGGA GGAAGAGTAT
    GAGACCAAAG GTGGCCGACG CAGGACCTGG GATGATGATT ATGTGCTGAA GAGGCAGTTT
    TCTGCTTTGG TCCCGGCTTT TGACCCACGC CCAGGGAGAA CTAATGTTCA GCAGACCACT
    GATCTTGAAA TTCCTGCTCC AGGCACGCCC CACTCTGAGC TACTGGAGGA GGTAGAATGT
    GCCCCAGCTC CACGATTAGC ACTCACCCTA AAGGTGACAG GCCTTGGGAG CGGGAGAGAG
    GTGGAGCTCC CCCTGAACAA TTTCCGTTCC ACCATATTTT TCTATGTACA GAGACTGCTG
    CAACTCTCCT GCAATGGAGC CATCAAAACT GACAAGCTGA GAAGGATTTG GGAGCCCACC
    TACACGATTA TGTACAGAGA GATGAAGGAT TCAGATAAGC AAAAAGAATG TGGCAGACTG
    GGCTGTTGGT CTGTAGAGCA TGTAGAACAA TCCCTAGGCA CAGATGCCTT GCCAAAGAAT
    GACCTGATCA CTTACCTGCA GCGAAATGCA GATCCAGGGT TCTTGCGACG CTGGAAACTG
    ACAGGAACCA ACAAAAGCAT TAGGAAAAAC CGCAACTGTT CCCAGCTTAT TGCTGCTTAC
    AAGGACTTCT GTGAGAATGG CTGCAAGTCT CTCTCAATGC CAGCTGCTCT TGCCACACTC
    CAGAGTGCTG ATATCCTTAG TCATTCCCGA GAGCAGGCAC AAGCCAAAGC GGGCAGCAGC
    CAAAACTCCT GCGGGGTAGA AGATGTTTTG CAGCTCCTGC GAATTCTCTT TATTGTAGCC
    AGTGACCCAT ATTCTGCCAG GACCCCACAG GAAGATGGAG AAGATATGCT CCTTTTCAGT
    GTTCCTCCAG AGGAGTTTAC TAGCAAGAAA ATCACCACAA AGATTGTTCA GCAAATCGAG
    GAACCTTTAG CTTTAGCAAG TGGAGCTTTG CCAGACTGGT GTGAACAGCT GACCAGCAAA
    TGTCCCTTCT TAATACCCTT TGAAACCCGG CAGCTTTACT TCACATGCAC AGCATTTGGT
    GCCTCAAGGG CTATTGTGTG GCTGCAGAAC AGGCGGGAAG CCACAGTTGA GAGGAGCAGA
    ACAGCAAGTG CAGTGCGCAG AGACGACCCA GGGGAGTTCA GAGTGGGCCG TCTAAAACAC
    GAACGTGTGA AGGTCCCACG AGGGGAGTCT TTGATGGAGT GGGCAGAGAA TGTTATGCAA
    ATCCACGCAG ACCGAAAATC TGTGCTTGAG GTGGAGTTTC TGGGAGAAGA AGGAACAGGC
    TTGGGCCCTA CCCTTGAGTT TTATGCCCTG GTGGCAGCAG AGTTCCAGAA AACAGATTTG
    GGGATCTGGT TATGTGATGA TGACTTCCCA GATGATGAGT CTCGGCAGGT GGATCTAGGT
    GGTGGTTTGA AGCCCCCTGG TTACTATGTG CAGCGTTCCT GTGGCCTCTT CATTGCACCC
    TACCCACAGG ATAGTGAGGA ACTAGACCGA GTGACCAGAC TTTGCCATTT CCTTGGTGTT
    TTCCTTGCAA AGTGCATCCA GGACAATAGA TTAGTGGACT TGCCCATTTC AAAGCCGTTT
    TTTAAGCTTA TGTGTATGGG TGACATTAAG AGCAACATGA GCAAGCTTTT GTATGCCTCT
    CGGGGAGAGG AGAGTGAGCA CTGCACGGAG AGCCAGTCGG AGGCCTCTAC TGAGGATGGG
    CATGATGCCC TTTCAGTAGG AAGCTTTGAG GAAGACTGCA AATCTGAGTT CATCCTAGAC
    CCACCCAAAC CAAAGCCACC AGCCTGGTTT CAGGGTATTT TAACTTGGGA GGACTTTGAG
    CTAATAAACC CACATCGTGC TCGCTTCCTT AGGGACATTA GGGAGTTGGC TGTAAAGAGG
    AGACAGATTC TGGGGAACCG TTGCTTATCT GAGGATGAGA AGAACACTCA ACTTCAGGAA
    CTTATGCTAA AGAATCCTTC TGGATCTGGG CCTCCAGTCA GCATTGAGGA TTTGGGACTC
    AATTTCCAGT TCTGTCCATC TTCCCGGGTT TATGGCTTCT CTGCAGTCGA TCTAAGGCCA
    AATGGAGAGG ATGAGATGGT GACGATAGAC AACGCTGAGG AGTATGTTGA TCTTATGTTT
    GATTTCTGCA TGCAGACTGG TGTACAAAAG CAAATGGAAG CATTTAGAAG TGGCTTTAAT
    AAAGTGTTCC CTATGGAGAA ACTGGGCTCC TTCAGCCCCG AGGAAGTCCA AATGATACTG
    TGTGGAAACC AGTCTCCCTC TTGGTCCGCT GAGGACATCA TCAATTACAC AGAACCAAAG
    CTAGGATATA CCCGAGAGAG TCCTGGGTTC CTTCGATTCG TGAGAGTCCT ATGTGGCATG
    TCGTCAGATG AGAGGAAAGC GTTCCTGCAG TTTACAACGG GGTGCTCCAC ATTGCCTCCA
    GGAGGACTGG CCAATCTGCA TCCGCGTCTC ACAGTCGTGC GCAAGGTTGA TGCCACGGAT
    GCCAGCTACC CATCTGTAAA TACATGTGTG CATTACCTGA AGCTGCCGGA GTATTCCTCC
    GAGGAAATCA TGAGAGACCG CCTGCTTGCT GCTACCATGG AGAAAGGATT CCATCTCAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa63529
    Clone ID Related Accession (Same CDS sequence) XM_012968095.3 , XM_012968096.3
    Accession Version XM_012968096.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012968096.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGGCTGACG TAGATCCAGA CACACTACTG GAATGGCTGC AGATGGGGCA GGGGGATGAG 
    AGAGACATGC AGCTCATTGC CTTGGAACAG CTCTGCATGC TGCTGCTCAT GTCGGATAAT
    GTGGATAGGT GTTTTGAAAC ATGCCCACCT CGCACCTTCC TGCCAGCCTT GTGTAAAATA
    TTCCTGGATG AAAGCGCCCC AGACAATGTG TTGGAAGTAA CAGCCCGTGC AATAACCTAT
    TACCTAGATG TGTCCGCCGA GTGCACGAGG CGTATTGTGG GAGTGGAAGG GGCTATCAAA
    GCTTTGTGCA ATAGGCTTGT GGTGGTGGAG CTCAACAACA GAACCAGTCG GGATTTGGCA
    GAACAGTGTG TCAAGGTGCT GGAGCTTATC TGTACACGTG AGTCTGGGGC TGTCTTTGAG
    GCAGGGGGCT TGAACTGCGT GTTGACATTC ATTCGGGACA GCGGACACCT AGTTCACAAG
    GATACACTGC ATTCTGCAAT GGCTGTGGTG TCCCGATTGT GTGGTAAGAT GGAGCCACAG
    GATGCATCAT TAGAAACGTG TGTGGAGTCT CTCTCCAGCC TGCTGAAGCA CGAAGACCAC
    CAGGTATCAG ATGGAGCTCT CCGCTGCTTT GCTTCCCTTG CTGACCGCTT TACACGCCGT
    GGTGTAGATC CAGCTCCTCT TGCACAGCAT GGGCTCACTG AGGAACTGCT GTCCCGAATG
    GCAGCAGCCG GAGGCACTGT ATCAGGTCCA TCTTCAGCCT GTAAAACAGG CCGTGGCACC
    AGTGGGGGAC CTTCCACCTC TGGAGACTCA AAAATCAGCA ATCAGGTCTC CACTATAGTC
    AGCCTGTTAT CAACACTATG CCGTGGTTCT CCAGTCGTCA CACATGATTT ACTGCGGGCA
    GAACTCTTAG ACTCCATGGA AAGTGCTCTT CAAGGTGACG AGCGTTGTGT GCTGGATACC
    ATGCGCCTTG TAGACCTTCT TTTGGTGCTG CTTTTTGAAG GCCGCAAAGC TCTGCCTAAG
    TCTAGTGCTG GGTCCACAGG TCGTATTCCT GGGCTGCGGC GGCTTGACAG CTCAGGAGAG
    CGATCTCACC GCCAACTGAT AGACTGCATC CGCAGCAAGG ACACAGATGC TCTCATAGAT
    GCCATTGATA CAGGTGCTTT TGAAGTAAAT TTTATGGATG ATGTTGGCCA GACTCTTCTG
    AATTGGGCCT CTGCTTTTGG CACACAGGAA ATGGTTGAAT TCCTGTGTGA GCGAGGTGCC
    GATGTAAATA GAGGGCAGAG GTCATCTTCA CTGCATTATG CTGCTTGCTT CGGAAGGCCT
    CAGGTGGCAA AGACCTTGCT TCGCCATGGA GCCAACCCAG ATTTAAGAGA TGAGGATGGA
    AAAACACCTT TGGATAAAGC CCGGGAGAGA GGACACAGCG AAGTGGTGGC CATTTTGCAG
    TCCCCAGGGG ACTGGATGTG CCCCGTAAAC AAAGGAGATG AAAAGAAAAA GAAAGACTCT
    AACCGGGAAG AGGAAGAATG CAATGAGCCA AAGGGAGACC CAGAAATGGC CCCCATCTAC
    CTAAAGAGGC TGCTGCCTGT CTTTGCACAG ACCTTTCAGC AAACCATGCT TCCCTCTATC
    AGGAAAGCAA GCTTAGCACT TATTCGGAAG ATGATCCATT TCTGCTCAGA AGCACTGCTT
    AAGGAAGTTT GTCACTCAGA CGCTGGACAC AATTTGCCCA CTGTTCTTGT TGAGATAACA
    GCCACTGTTT TAGACCAGGA GGACGATGAT GATGGTCACC TTCTGGCACT TCAAATCATT
    AGGGATTTGG TGGACAAAGG TGATGACCTT TTTCTTGATC AATTGGCCCG GCTTGGTGTT
    ATAAGCAAGG TTTCTACACT AGCTGGACCT ACATCAGATG ATGAGAATGA GGAGGATAGC
    AAGCCAGAGA AGGTGAGGAT ACACCTATTT CCTCTGTACA GTGCCCACAA AGGAACACAA
    GCAGCTGCAT CACAACTTAA AGAGGATGAG CCACAGGAAG ATGCCAAGGA GTTGCAACAG
    GGCCGGCCCT ACCATTGGCG GGACTGGTCT GTTATTCGCG GTAGGGACTG TTTGTACATC
    TGGAGTGATG CTGCAGCTCT GGAGCTCTCT AATGGTAGCA ATGGCTGGTT CCGCTTTATC
    CTAGATGGGA AACTGGCAAC CATGTATTCA AGTGGAAGCC CAGAGGGGGG TTCTGATAGC
    TCAGAAAGTA GAAGTGAATT TTTAGAAAAG TTACAGCGGG CCCGCAGCCA GGTCAAACCA
    TCCACTTCTA GCCAGCCTAT CTTGTCCACT CCTGGTCCAG GAAAGCTAAC AGTAGGCAAC
    TGGTCTCTCA CTTGTCTGAA AGATGGTGAG ATTGCAATCC ACAACTCGGA CGGGCAGCAG
    GCTACAATAC TGAAGGAGGA TCTACCAGGC TTTGTGTTTG AGTCAAACAG GGGTACCAAG
    CACTCCTTCA CGGCAGAAAC ATCACTAGGA TCTGAATTTG TAACTGGCTG GACAGGAAAG
    AGAGGCAGAA AGCTGAAGTC TAAGTTGGAG AAAACTAAAC AGAAGGTACG GACAATGGCA
    AGAGATTTGT ATGATGATCA TTTTAAGGCA GTGGAAAGCA TGCCCCGAGG AGTGGTAGTG
    ACGCTCCGAA ATATTGCAAC ACAGCTGGAA TCTGCTTGGG AACTTCATAC AAACAGACAG
    TGTATTGAAG GAGAGAACAC ATGGAGAGAT CTGATGAAAA CTGCCCTGGA AAACCTGATT
    GTGCTACTGA AGGATGAGAA TACTATTTCC CCTTATGAGA TGTGCAGCAG TGGACTGGTG
    CAGGCTTTGC TTACGGTGCT CAATAATAAT GAAGACTGTG ATATAAAACA AGACTGTGGG
    CAGCTGGTGG AGAGACTTAA TGTATTTAAA ACTGCCTTCA GCGAAAATGA AGATGATGAA
    AGCCGCCCTG CCGTTGCCCT CGTCCGGAAG TTGATTGCTG TGCTTGAATC AATAGAACGG
    CTGCCCCTTC ACCTGTATGA CACCCCTGGA TCTAGCTATA ACTTGCAGAT CCTGACCCGT
    AGGCTTCGGT TTCGGCTGGA GCGTGCACCT GGAGAAACGT CTCTAATCGA CCGTACCGGT
    CGGATGCTGA AAATGGAGCC ACTGGCAACT GTAGAGTCCT TGGAGCAGTA TTTACTGAAA
    ATGGTGGCTA AGCAGTGGTA CGACTTTGAC CGTTCGTCCT TTGTGTTTGT GCGCAAATTG
    CGCGAGGGCC AGAGTTGTGT GTTCCGCCAC CAGCATGACT TTGATGATAA TGGAATCATG
    TACTGGATTG GCACCAATGC AAAGACAGCT TATGAGTGGG TCAACCCTGC TGCTTACGGA
    CTAGTAGTTG TTACATCTTC TGAAGGCCGC AATTTGCCAT ATGGCCGCCT TGAGGATATC
    CTGAGCCGTG ATAGCTCTGC CCTTAATTGC CACACTAATG ATGATAAGAG TGCCTGGTTT
    GCCATTGACC TTGGCTTGTG GGTTGTGCCT TCTGCCTATA CACTGCGTCA TGCTCGTGGA
    TATGGCCGTT CCGCTCTTCG CAATTGGGTC TTCCAGGTGT CTAAAGATGG TCAGAACTGG
    ACCACCCTCT ACACCCACAT GGATGACTGC AGCCTGAATG AACCTGGCTC CACAGCCACA
    TGGCCTCTGG ACCCTGCGAG AGAAGAAAAG CAGGGTTGGC GTCATGTCCG GATAAAGCAG
    ACGGGAAAGA ATGCCAGTGG CCAGACCCAC TACCTCTCAC TTTCTGGCTT TGAGCTTTAT
    GGCAATGTGA CTGGGGTGTG TGAAGACCAA CTTGGTAAAG CTGCCAAAGA GGCAGAAGCC
    AACTTAAGGC GCCAGAGGCG GCTGGTACGC TCTCAGGTGC TAAAATATAT GGTGCCTGGA
    GCCCGTGTTA TTCGGGGCAT TGATTGGAAG TGGCGAGACC AAGATGGCAG TGCTCAAGGA
    GAAGGCACTG TTACAGGAGA ACTGCACAAT GGCTGGATTG ATGTCATATG GGATGCCGGT
    GGCTCTAACT CTTACCGCAT GGGCGCGGAA GGAAAATTTG ACCTGAAGCT GGCCCCGGGT
    TATGACCCAG AGTCTGCGGT GTCACCCAAA CCTGTTTCAT CCACTGTTGC AGGAACACCG
    CCCTCGTGGA GCAGCCTGGT GAAAAACAAC TGTCCTGACA AAGCCCCTCC CTCTTCCTCA
    TCCTCTTGTG TGGTCGTTGG AAGCGTGGCG GGCTCAGGCA GCCGCAAAGG AAGCAGCAGC
    TCGGTCTGTA GCGTGGCAAG CAGCAGCGAC GTAAGCCTAA GCTGTGCAAA GACAGAGAGG
    AGGGCAGAGG AGCAAGTCTC CGACATTCAC CATGATCCCA TCCTGCTTCT GTCCTCTAAC
    CAAGCAGCCT CGGGTTCCTC CACCTGCCCC CCTGGAGGAG AGACTGTGGG TGAAGGTGGA
    GATAGAAAGG CAGGAGAAGC ACCAGCAATC TCAATGGGTA TGGTGAGCAT TAGCTCCCCT
    GATGTCAGTT CCGTTTCCGA ACTCAGCAAC AAGGAAGTGG CAGTTCCACG ACCACTGGGA
    TCTTCTGCCA GCAACAGATT GTCTGTCAGT TCACTGTTGG CAGCTGGGGC ACCCATGAGT
    TCTAGCGCCA GTGTACCAAA CTTGTCCTCC AGGGAAACGT CCAGTCTGGA GTCCTTTGTA
    AGGAGAGTGG CAAATATCGC CAGGACAAAT GCAACCAACA ACATGAACCT GAGTCGTAGC
    AGCAGTGATA ACAATACCAA CACTCTGGGG AGGAATGCAG TCAGCTCAGC CACTTCACCA
    CTTATGGGAG CTCAGAGCTT TCCCAACCTG ACGACAACAG GAACCACATC CACCGTTACA
    ATGTCTACAT CCAGTGTCAC CAGCAGTAAT GTGGCAACAG CCACTACAGG GTTGTCTGTA
    GGTCAGTCCC TCAGTAACAC CCTGACTACA AGCCTAACCT CGACATCCAG TGAGAGTGAC
    ACTGGGCAGG AGGCAGAATA CTCCCTGTAT GACTTTCTTG ATAGCTGCCG TGCCAGCACT
    TTGCTTGCTG AACTTGATGA TGACGAGGAT CTCCCTGAGC CCGATGAGGA AGATGATGAA
    AACGAAGATG ATAATCAGGA AGAGCAAGAG TATGAAGAAG TTATGGAGGA GGAAGAGTAT
    GAGACCAAAG GTGGCCGACG CAGGACCTGG GATGATGATT ATGTGCTGAA GAGGCAGTTT
    TCTGCTTTGG TCCCGGCTTT TGACCCACGC CCAGGGAGAA CTAATGTTCA GCAGACCACT
    GATCTTGAAA TTCCTGCTCC AGGCACGCCC CACTCTGAGC TACTGGAGGA GGTAGAATGT
    GCCCCAGCTC CACGATTAGC ACTCACCCTA AAGGTGACAG GCCTTGGGAG CGGGAGAGAG
    GTGGAGCTCC CCCTGAACAA TTTCCGTTCC ACCATATTTT TCTATGTACA GAGACTGCTG
    CAACTCTCCT GCAATGGAGC CATCAAAACT GACAAGCTGA GAAGGATTTG GGAGCCCACC
    TACACGATTA TGTACAGAGA GATGAAGGAT TCAGATAAGC AAAAAGAATG TGGCAGACTG
    GGCTGTTGGT CTGTAGAGCA TGTAGAACAA TCCCTAGGCA CAGATGCCTT GCCAAAGAAT
    GACCTGATCA CTTACCTGCA GCGAAATGCA GATCCAGGGT TCTTGCGACG CTGGAAACTG
    ACAGGAACCA ACAAAAGCAT TAGGAAAAAC CGCAACTGTT CCCAGCTTAT TGCTGCTTAC
    AAGGACTTCT GTGAGAATGG CTGCAAGTCT CTCTCAATGC CAGCTGCTCT TGCCACACTC
    CAGAGTGCTG ATATCCTTAG TCATTCCCGA GAGCAGGCAC AAGCCAAAGC GGGCAGCAGC
    CAAAACTCCT GCGGGGTAGA AGATGTTTTG CAGCTCCTGC GAATTCTCTT TATTGTAGCC
    AGTGACCCAT ATTCTGCCAG GACCCCACAG GAAGATGGAG AAGATATGCT CCTTTTCAGT
    GTTCCTCCAG AGGAGTTTAC TAGCAAGAAA ATCACCACAA AGATTGTTCA GCAAATCGAG
    GAACCTTTAG CTTTAGCAAG TGGAGCTTTG CCAGACTGGT GTGAACAGCT GACCAGCAAA
    TGTCCCTTCT TAATACCCTT TGAAACCCGG CAGCTTTACT TCACATGCAC AGCATTTGGT
    GCCTCAAGGG CTATTGTGTG GCTGCAGAAC AGGCGGGAAG CCACAGTTGA GAGGAGCAGA
    ACAGCAAGTG CAGTGCGCAG AGACGACCCA GGGGAGTTCA GAGTGGGCCG TCTAAAACAC
    GAACGTGTGA AGGTCCCACG AGGGGAGTCT TTGATGGAGT GGGCAGAGAA TGTTATGCAA
    ATCCACGCAG ACCGAAAATC TGTGCTTGAG GTGGAGTTTC TGGGAGAAGA AGGAACAGGC
    TTGGGCCCTA CCCTTGAGTT TTATGCCCTG GTGGCAGCAG AGTTCCAGAA AACAGATTTG
    GGGATCTGGT TATGTGATGA TGACTTCCCA GATGATGAGT CTCGGCAGGT GGATCTAGGT
    GGTGGTTTGA AGCCCCCTGG TTACTATGTG CAGCGTTCCT GTGGCCTCTT CATTGCACCC
    TACCCACAGG ATAGTGAGGA ACTAGACCGA GTGACCAGAC TTTGCCATTT CCTTGGTGTT
    TTCCTTGCAA AGTGCATCCA GGACAATAGA TTAGTGGACT TGCCCATTTC AAAGCCGTTT
    TTTAAGCTTA TGTGTATGGG TGACATTAAG AGCAACATGA GCAAGCTTTT GTATGCCTCT
    CGGGGAGAGG AGAGTGAGCA CTGCACGGAG AGCCAGTCGG AGGCCTCTAC TGAGGATGGG
    CATGATGCCC TTTCAGTAGG AAGCTTTGAG GAAGACTGCA AATCTGAGTT CATCCTAGAC
    CCACCCAAAC CAAAGCCACC AGCCTGGTTT CAGGGTATTT TAACTTGGGA GGACTTTGAG
    CTAATAAACC CACATCGTGC TCGCTTCCTT AGGGACATTA GGGAGTTGGC TGTAAAGAGG
    AGACAGATTC TGGGGAACCG TTGCTTATCT GAGGATGAGA AGAACACTCA ACTTCAGGAA
    CTTATGCTAA AGAATCCTTC TGGATCTGGG CCTCCAGTCA GCATTGAGGA TTTGGGACTC
    AATTTCCAGT TCTGTCCATC TTCCCGGGTT TATGGCTTCT CTGCAGTCGA TCTAAGGCCA
    AATGGAGAGG ATGAGATGGT GACGATAGAC AACGCTGAGG AGTATGTTGA TCTTATGTTT
    GATTTCTGCA TGCAGACTGG TGTACAAAAG CAAATGGAAG CATTTAGAAG TGGCTTTAAT
    AAAGTGTTCC CTATGGAGAA ACTGGGCTCC TTCAGCCCCG AGGAAGTCCA AATGATACTG
    TGTGGAAACC AGTCTCCCTC TTGGTCCGCT GAGGACATCA TCAATTACAC AGAACCAAAG
    CTAGGATATA CCCGAGAGAG TCCTGGGTTC CTTCGATTCG TGAGAGTCCT ATGTGGCATG
    TCGTCAGATG AGAGGAAAGC GTTCCTGCAG TTTACAACGG GGTGCTCCAC ATTGCCTCCA
    GGAGGACTGG CCAATCTGCA TCCGCGTCTC ACAGTCGTGC GCAAGGTTGA TGCCACGGAT
    GCCAGCTACC CATCTGTAAA TACATGTGTG CATTACCTGA AGCTGCCGGA GTATTCCTCC
    GAGGAAATCA TGAGAGACCG CCTGCTTGCT GCTACCATGG AGAAAGGATT CCATCTCAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012823550.1
    CDS205..7947
    Translation

    Target ORF information:

    RefSeq Version XM_012968096.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 1 (hectd1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012968096.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGGCTGACG TAGATCCAGA CACACTACTG GAATGGCTGC AGATGGGGCA GGGGGATGAG 
    AGAGACATGC AGCTCATTGC CTTGGAACAG CTCTGCATGC TGCTGCTCAT GTCGGATAAT
    GTGGATAGGT GTTTTGAAAC ATGCCCACCT CGCACCTTCC TGCCAGCCTT GTGTAAAATA
    TTCCTGGATG AAAGCGCCCC AGACAATGTG TTGGAAGTAA CAGCCCGTGC AATAACCTAT
    TACCTAGATG TGTCCGCCGA GTGCACGAGG CGTATTGTGG GAGTGGAAGG GGCTATCAAA
    GCTTTGTGCA ATAGGCTTGT GGTGGTGGAG CTCAACAACA GAACCAGTCG GGATTTGGCA
    GAACAGTGTG TCAAGGTGCT GGAGCTTATC TGTACACGTG AGTCTGGGGC TGTCTTTGAG
    GCAGGGGGCT TGAACTGCGT GTTGACATTC ATTCGGGACA GCGGACACCT AGTTCACAAG
    GATACACTGC ATTCTGCAAT GGCTGTGGTG TCCCGATTGT GTGGTAAGAT GGAGCCACAG
    GATGCATCAT TAGAAACGTG TGTGGAGTCT CTCTCCAGCC TGCTGAAGCA CGAAGACCAC
    CAGGTATCAG ATGGAGCTCT CCGCTGCTTT GCTTCCCTTG CTGACCGCTT TACACGCCGT
    GGTGTAGATC CAGCTCCTCT TGCACAGCAT GGGCTCACTG AGGAACTGCT GTCCCGAATG
    GCAGCAGCCG GAGGCACTGT ATCAGGTCCA TCTTCAGCCT GTAAAACAGG CCGTGGCACC
    AGTGGGGGAC CTTCCACCTC TGGAGACTCA AAAATCAGCA ATCAGGTCTC CACTATAGTC
    AGCCTGTTAT CAACACTATG CCGTGGTTCT CCAGTCGTCA CACATGATTT ACTGCGGGCA
    GAACTCTTAG ACTCCATGGA AAGTGCTCTT CAAGGTGACG AGCGTTGTGT GCTGGATACC
    ATGCGCCTTG TAGACCTTCT TTTGGTGCTG CTTTTTGAAG GCCGCAAAGC TCTGCCTAAG
    TCTAGTGCTG GGTCCACAGG TCGTATTCCT GGGCTGCGGC GGCTTGACAG CTCAGGAGAG
    CGATCTCACC GCCAACTGAT AGACTGCATC CGCAGCAAGG ACACAGATGC TCTCATAGAT
    GCCATTGATA CAGGTGCTTT TGAAGTAAAT TTTATGGATG ATGTTGGCCA GACTCTTCTG
    AATTGGGCCT CTGCTTTTGG CACACAGGAA ATGGTTGAAT TCCTGTGTGA GCGAGGTGCC
    GATGTAAATA GAGGGCAGAG GTCATCTTCA CTGCATTATG CTGCTTGCTT CGGAAGGCCT
    CAGGTGGCAA AGACCTTGCT TCGCCATGGA GCCAACCCAG ATTTAAGAGA TGAGGATGGA
    AAAACACCTT TGGATAAAGC CCGGGAGAGA GGACACAGCG AAGTGGTGGC CATTTTGCAG
    TCCCCAGGGG ACTGGATGTG CCCCGTAAAC AAAGGAGATG AAAAGAAAAA GAAAGACTCT
    AACCGGGAAG AGGAAGAATG CAATGAGCCA AAGGGAGACC CAGAAATGGC CCCCATCTAC
    CTAAAGAGGC TGCTGCCTGT CTTTGCACAG ACCTTTCAGC AAACCATGCT TCCCTCTATC
    AGGAAAGCAA GCTTAGCACT TATTCGGAAG ATGATCCATT TCTGCTCAGA AGCACTGCTT
    AAGGAAGTTT GTCACTCAGA CGCTGGACAC AATTTGCCCA CTGTTCTTGT TGAGATAACA
    GCCACTGTTT TAGACCAGGA GGACGATGAT GATGGTCACC TTCTGGCACT TCAAATCATT
    AGGGATTTGG TGGACAAAGG TGATGACCTT TTTCTTGATC AATTGGCCCG GCTTGGTGTT
    ATAAGCAAGG TTTCTACACT AGCTGGACCT ACATCAGATG ATGAGAATGA GGAGGATAGC
    AAGCCAGAGA AGGTGAGGAT ACACCTATTT CCTCTGTACA GTGCCCACAA AGGAACACAA
    GCAGCTGCAT CACAACTTAA AGAGGATGAG CCACAGGAAG ATGCCAAGGA GTTGCAACAG
    GGCCGGCCCT ACCATTGGCG GGACTGGTCT GTTATTCGCG GTAGGGACTG TTTGTACATC
    TGGAGTGATG CTGCAGCTCT GGAGCTCTCT AATGGTAGCA ATGGCTGGTT CCGCTTTATC
    CTAGATGGGA AACTGGCAAC CATGTATTCA AGTGGAAGCC CAGAGGGGGG TTCTGATAGC
    TCAGAAAGTA GAAGTGAATT TTTAGAAAAG TTACAGCGGG CCCGCAGCCA GGTCAAACCA
    TCCACTTCTA GCCAGCCTAT CTTGTCCACT CCTGGTCCAG GAAAGCTAAC AGTAGGCAAC
    TGGTCTCTCA CTTGTCTGAA AGATGGTGAG ATTGCAATCC ACAACTCGGA CGGGCAGCAG
    GCTACAATAC TGAAGGAGGA TCTACCAGGC TTTGTGTTTG AGTCAAACAG GGGTACCAAG
    CACTCCTTCA CGGCAGAAAC ATCACTAGGA TCTGAATTTG TAACTGGCTG GACAGGAAAG
    AGAGGCAGAA AGCTGAAGTC TAAGTTGGAG AAAACTAAAC AGAAGGTACG GACAATGGCA
    AGAGATTTGT ATGATGATCA TTTTAAGGCA GTGGAAAGCA TGCCCCGAGG AGTGGTAGTG
    ACGCTCCGAA ATATTGCAAC ACAGCTGGAA TCTGCTTGGG AACTTCATAC AAACAGACAG
    TGTATTGAAG GAGAGAACAC ATGGAGAGAT CTGATGAAAA CTGCCCTGGA AAACCTGATT
    GTGCTACTGA AGGATGAGAA TACTATTTCC CCTTATGAGA TGTGCAGCAG TGGACTGGTG
    CAGGCTTTGC TTACGGTGCT CAATAATAAT GAAGACTGTG ATATAAAACA AGACTGTGGG
    CAGCTGGTGG AGAGACTTAA TGTATTTAAA ACTGCCTTCA GCGAAAATGA AGATGATGAA
    AGCCGCCCTG CCGTTGCCCT CGTCCGGAAG TTGATTGCTG TGCTTGAATC AATAGAACGG
    CTGCCCCTTC ACCTGTATGA CACCCCTGGA TCTAGCTATA ACTTGCAGAT CCTGACCCGT
    AGGCTTCGGT TTCGGCTGGA GCGTGCACCT GGAGAAACGT CTCTAATCGA CCGTACCGGT
    CGGATGCTGA AAATGGAGCC ACTGGCAACT GTAGAGTCCT TGGAGCAGTA TTTACTGAAA
    ATGGTGGCTA AGCAGTGGTA CGACTTTGAC CGTTCGTCCT TTGTGTTTGT GCGCAAATTG
    CGCGAGGGCC AGAGTTGTGT GTTCCGCCAC CAGCATGACT TTGATGATAA TGGAATCATG
    TACTGGATTG GCACCAATGC AAAGACAGCT TATGAGTGGG TCAACCCTGC TGCTTACGGA
    CTAGTAGTTG TTACATCTTC TGAAGGCCGC AATTTGCCAT ATGGCCGCCT TGAGGATATC
    CTGAGCCGTG ATAGCTCTGC CCTTAATTGC CACACTAATG ATGATAAGAG TGCCTGGTTT
    GCCATTGACC TTGGCTTGTG GGTTGTGCCT TCTGCCTATA CACTGCGTCA TGCTCGTGGA
    TATGGCCGTT CCGCTCTTCG CAATTGGGTC TTCCAGGTGT CTAAAGATGG TCAGAACTGG
    ACCACCCTCT ACACCCACAT GGATGACTGC AGCCTGAATG AACCTGGCTC CACAGCCACA
    TGGCCTCTGG ACCCTGCGAG AGAAGAAAAG CAGGGTTGGC GTCATGTCCG GATAAAGCAG
    ACGGGAAAGA ATGCCAGTGG CCAGACCCAC TACCTCTCAC TTTCTGGCTT TGAGCTTTAT
    GGCAATGTGA CTGGGGTGTG TGAAGACCAA CTTGGTAAAG CTGCCAAAGA GGCAGAAGCC
    AACTTAAGGC GCCAGAGGCG GCTGGTACGC TCTCAGGTGC TAAAATATAT GGTGCCTGGA
    GCCCGTGTTA TTCGGGGCAT TGATTGGAAG TGGCGAGACC AAGATGGCAG TGCTCAAGGA
    GAAGGCACTG TTACAGGAGA ACTGCACAAT GGCTGGATTG ATGTCATATG GGATGCCGGT
    GGCTCTAACT CTTACCGCAT GGGCGCGGAA GGAAAATTTG ACCTGAAGCT GGCCCCGGGT
    TATGACCCAG AGTCTGCGGT GTCACCCAAA CCTGTTTCAT CCACTGTTGC AGGAACACCG
    CCCTCGTGGA GCAGCCTGGT GAAAAACAAC TGTCCTGACA AAGCCCCTCC CTCTTCCTCA
    TCCTCTTGTG TGGTCGTTGG AAGCGTGGCG GGCTCAGGCA GCCGCAAAGG AAGCAGCAGC
    TCGGTCTGTA GCGTGGCAAG CAGCAGCGAC GTAAGCCTAA GCTGTGCAAA GACAGAGAGG
    AGGGCAGAGG AGCAAGTCTC CGACATTCAC CATGATCCCA TCCTGCTTCT GTCCTCTAAC
    CAAGCAGCCT CGGGTTCCTC CACCTGCCCC CCTGGAGGAG AGACTGTGGG TGAAGGTGGA
    GATAGAAAGG CAGGAGAAGC ACCAGCAATC TCAATGGGTA TGGTGAGCAT TAGCTCCCCT
    GATGTCAGTT CCGTTTCCGA ACTCAGCAAC AAGGAAGTGG CAGTTCCACG ACCACTGGGA
    TCTTCTGCCA GCAACAGATT GTCTGTCAGT TCACTGTTGG CAGCTGGGGC ACCCATGAGT
    TCTAGCGCCA GTGTACCAAA CTTGTCCTCC AGGGAAACGT CCAGTCTGGA GTCCTTTGTA
    AGGAGAGTGG CAAATATCGC CAGGACAAAT GCAACCAACA ACATGAACCT GAGTCGTAGC
    AGCAGTGATA ACAATACCAA CACTCTGGGG AGGAATGCAG TCAGCTCAGC CACTTCACCA
    CTTATGGGAG CTCAGAGCTT TCCCAACCTG ACGACAACAG GAACCACATC CACCGTTACA
    ATGTCTACAT CCAGTGTCAC CAGCAGTAAT GTGGCAACAG CCACTACAGG GTTGTCTGTA
    GGTCAGTCCC TCAGTAACAC CCTGACTACA AGCCTAACCT CGACATCCAG TGAGAGTGAC
    ACTGGGCAGG AGGCAGAATA CTCCCTGTAT GACTTTCTTG ATAGCTGCCG TGCCAGCACT
    TTGCTTGCTG AACTTGATGA TGACGAGGAT CTCCCTGAGC CCGATGAGGA AGATGATGAA
    AACGAAGATG ATAATCAGGA AGAGCAAGAG TATGAAGAAG TTATGGAGGA GGAAGAGTAT
    GAGACCAAAG GTGGCCGACG CAGGACCTGG GATGATGATT ATGTGCTGAA GAGGCAGTTT
    TCTGCTTTGG TCCCGGCTTT TGACCCACGC CCAGGGAGAA CTAATGTTCA GCAGACCACT
    GATCTTGAAA TTCCTGCTCC AGGCACGCCC CACTCTGAGC TACTGGAGGA GGTAGAATGT
    GCCCCAGCTC CACGATTAGC ACTCACCCTA AAGGTGACAG GCCTTGGGAG CGGGAGAGAG
    GTGGAGCTCC CCCTGAACAA TTTCCGTTCC ACCATATTTT TCTATGTACA GAGACTGCTG
    CAACTCTCCT GCAATGGAGC CATCAAAACT GACAAGCTGA GAAGGATTTG GGAGCCCACC
    TACACGATTA TGTACAGAGA GATGAAGGAT TCAGATAAGC AAAAAGAATG TGGCAGACTG
    GGCTGTTGGT CTGTAGAGCA TGTAGAACAA TCCCTAGGCA CAGATGCCTT GCCAAAGAAT
    GACCTGATCA CTTACCTGCA GCGAAATGCA GATCCAGGGT TCTTGCGACG CTGGAAACTG
    ACAGGAACCA ACAAAAGCAT TAGGAAAAAC CGCAACTGTT CCCAGCTTAT TGCTGCTTAC
    AAGGACTTCT GTGAGAATGG CTGCAAGTCT CTCTCAATGC CAGCTGCTCT TGCCACACTC
    CAGAGTGCTG ATATCCTTAG TCATTCCCGA GAGCAGGCAC AAGCCAAAGC GGGCAGCAGC
    CAAAACTCCT GCGGGGTAGA AGATGTTTTG CAGCTCCTGC GAATTCTCTT TATTGTAGCC
    AGTGACCCAT ATTCTGCCAG GACCCCACAG GAAGATGGAG AAGATATGCT CCTTTTCAGT
    GTTCCTCCAG AGGAGTTTAC TAGCAAGAAA ATCACCACAA AGATTGTTCA GCAAATCGAG
    GAACCTTTAG CTTTAGCAAG TGGAGCTTTG CCAGACTGGT GTGAACAGCT GACCAGCAAA
    TGTCCCTTCT TAATACCCTT TGAAACCCGG CAGCTTTACT TCACATGCAC AGCATTTGGT
    GCCTCAAGGG CTATTGTGTG GCTGCAGAAC AGGCGGGAAG CCACAGTTGA GAGGAGCAGA
    ACAGCAAGTG CAGTGCGCAG AGACGACCCA GGGGAGTTCA GAGTGGGCCG TCTAAAACAC
    GAACGTGTGA AGGTCCCACG AGGGGAGTCT TTGATGGAGT GGGCAGAGAA TGTTATGCAA
    ATCCACGCAG ACCGAAAATC TGTGCTTGAG GTGGAGTTTC TGGGAGAAGA AGGAACAGGC
    TTGGGCCCTA CCCTTGAGTT TTATGCCCTG GTGGCAGCAG AGTTCCAGAA AACAGATTTG
    GGGATCTGGT TATGTGATGA TGACTTCCCA GATGATGAGT CTCGGCAGGT GGATCTAGGT
    GGTGGTTTGA AGCCCCCTGG TTACTATGTG CAGCGTTCCT GTGGCCTCTT CATTGCACCC
    TACCCACAGG ATAGTGAGGA ACTAGACCGA GTGACCAGAC TTTGCCATTT CCTTGGTGTT
    TTCCTTGCAA AGTGCATCCA GGACAATAGA TTAGTGGACT TGCCCATTTC AAAGCCGTTT
    TTTAAGCTTA TGTGTATGGG TGACATTAAG AGCAACATGA GCAAGCTTTT GTATGCCTCT
    CGGGGAGAGG AGAGTGAGCA CTGCACGGAG AGCCAGTCGG AGGCCTCTAC TGAGGATGGG
    CATGATGCCC TTTCAGTAGG AAGCTTTGAG GAAGACTGCA AATCTGAGTT CATCCTAGAC
    CCACCCAAAC CAAAGCCACC AGCCTGGTTT CAGGGTATTT TAACTTGGGA GGACTTTGAG
    CTAATAAACC CACATCGTGC TCGCTTCCTT AGGGACATTA GGGAGTTGGC TGTAAAGAGG
    AGACAGATTC TGGGGAACCG TTGCTTATCT GAGGATGAGA AGAACACTCA ACTTCAGGAA
    CTTATGCTAA AGAATCCTTC TGGATCTGGG CCTCCAGTCA GCATTGAGGA TTTGGGACTC
    AATTTCCAGT TCTGTCCATC TTCCCGGGTT TATGGCTTCT CTGCAGTCGA TCTAAGGCCA
    AATGGAGAGG ATGAGATGGT GACGATAGAC AACGCTGAGG AGTATGTTGA TCTTATGTTT
    GATTTCTGCA TGCAGACTGG TGTACAAAAG CAAATGGAAG CATTTAGAAG TGGCTTTAAT
    AAAGTGTTCC CTATGGAGAA ACTGGGCTCC TTCAGCCCCG AGGAAGTCCA AATGATACTG
    TGTGGAAACC AGTCTCCCTC TTGGTCCGCT GAGGACATCA TCAATTACAC AGAACCAAAG
    CTAGGATATA CCCGAGAGAG TCCTGGGTTC CTTCGATTCG TGAGAGTCCT ATGTGGCATG
    TCGTCAGATG AGAGGAAAGC GTTCCTGCAG TTTACAACGG GGTGCTCCAC ATTGCCTCCA
    GGAGGACTGG CCAATCTGCA TCCGCGTCTC ACAGTCGTGC GCAAGGTTGA TGCCACGGAT
    GCCAGCTACC CATCTGTAAA TACATGTGTG CATTACCTGA AGCTGCCGGA GTATTCCTCC
    GAGGAAATCA TGAGAGACCG CCTGCTTGCT GCTACCATGG AGAAAGGATT CCATCTCAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa63530
    Clone ID Related Accession (Same CDS sequence) XM_012968097.3
    Accession Version XM_012968097.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7674bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012968097.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGCTGACG TAGATCCAGA CACACTACTG GAATGGCTGC AGATGGGGCA GGGGGATGAG 
    AGAGACATGC AGCTCATTGC CTTGGAACAG CTCTGCATGC TGCTGCTCAT GTCGGATAAT
    GTGGATAGGT GTTTTGAAAC ATGCCCACCT CGCACCTTCC TGCCAGCCTT GTGTAAAATA
    TTCCTGGATG AAAGCGCCCC AGACAATGTG TTGGAAGTAA CAGCCCGTGC AATAACCTAT
    TACCTAGATG TGTCCGCCGA GTGCACGAGG CGTATTGTGG GAGTGGAAGG GGCTATCAAA
    GCTTTGTGCA ATAGGCTTGT GGTGGTGGAG CTCAACAACA GAACCAGTCG GGATTTGGCA
    GAACAGTGTG TCAAGGTGCT GGAGCTTATC TGTACACGTG AGTCTGGGGC TGTCTTTGAG
    GCAGGGGGCT TGAACTGCGT GTTGACATTC ATTCGGGACA GCGGACACCT AGTTCACAAG
    GATACACTGC ATTCTGCAAT GGCTGTGGTG TCCCGATTGT GTGGTAAGAT GGAGCCACAG
    GATGCATCAT TAGAAACGTG TGTGGAGTCT CTCTCCAGCC TGCTGAAGCA CGAAGACCAC
    CAGGTATCAG ATGGAGCTCT CCGCTGCTTT GCTTCCCTTG CTGACCGCTT TACACGCCGT
    GGTGTAGATC CAGCTCCTCT TGCACAGCAT GGGCTCACTG AGGAACTGCT GTCCCGAATG
    GCAGCAGCCG GAGGCACTGT ATCAGGTCCA TCTTCAGCCT GTAAAACAGG CCGTGGCACC
    AGTGGGGGAC CTTCCACCTC TGGAGACTCA AAAATCAGCA ATCAGGTCTC CACTATAGTC
    AGCCTGTTAT CAACACTATG CCGTGGTTCT CCAGTCGTCA CACATGATTT ACTGCGGGCA
    GAACTCTTAG ACTCCATGGA AAGTGCTCTT CAAGGTGACG AGCGTTGTGT GCTGGATACC
    ATGCGCCTTG TAGACCTTCT TTTGGTGCTG CTTTTTGAAG GCCGCAAAGC TCTGCCTAAG
    TCTAGTGCTG GGTCCACAGG TCGTATTCCT GGGCTGCGGC GGCTTGACAG CTCAGGAGAG
    CGATCTCACC GCCAACTGAT AGACTGCATC CGCAGCAAGG ACACAGATGC TCTCATAGAT
    GCCATTGATA CAGGTGCTTT TGAAGTAAAT TTTATGGATG ATGTTGGCCA GACTCTTCTG
    AATTGGGCCT CTGCTTTTGG CACACAGGAA ATGGTTGAAT TCCTGTGTGA GCGAGGTGCC
    GATGTAAATA GAGGGCAGAG GTCATCTTCA CTGCATTATG CTGCTTGCTT CGGAAGGCCT
    CAGGTGGCAA AGACCTTGCT TCGCCATGGA GCCAACCCAG ATTTAAGAGA TGAGGATGGA
    AAAACACCTT TGGATAAAGC CCGGGAGAGA GGACACAGCG AAGTGGTGGC CATTTTGCAG
    TCCCCAGGGG ACTGGATGTG CCCCGTAAAC AAAGGAGATG AAAAGAAAAA GAAAGACTCT
    AACCGGGAAG AGGAAGAATG CAATGAGCCA AAGGGAGACC CAGAAATGGC CCCCATCTAC
    CTAAAGAGGC TGCTGCCTGT CTTTGCACAG ACCTTTCAGC AAACCATGCT TCCCTCTATC
    AGGAAAGCAA GCTTAGCACT TATTCGGAAG ATGATCCATT TCTGCTCAGA AGCACTGCTT
    AAGGAAGTTT GTCACTCAGA CGCTGGACAC AATTTGCCCA CTGTTCTTGT TGAGATAACA
    GCCACTGTTT TAGACCAGGA GGACGATGAT GATGGTCACC TTCTGGCACT TCAAATCATT
    AGGGATTTGG TGGACAAAGG TGATGACCTT TTTCTTGATC AATTGGCCCG GCTTGGTGTT
    ATAAGCAAGG TTTCTACACT AGCTGGACCT ACATCAGATG ATGAGAATGA GGAGGATAGC
    AAGCCAGAGA AGGAGGATGA GCCACAGGAA GATGCCAAGG AGTTGCAACA GGGCCGGCCC
    TACCATTGGC GGGACTGGTC TGTTATTCGC GGTAGGGACT GTTTGTACAT CTGGAGTGAT
    GCTGCAGCTC TGGAGCTCTC TAATGGTAGC AATGGCTGGT TCCGCTTTAT CCTAGATGGG
    AAACTGGCAA CCATGTATTC AAGTGGAAGC CCAGAGGGGG GTTCTGATAG CTCAGAAAGT
    AGAAGTGAAT TTTTAGAAAA GTTACAGCGG GCCCGCAGCC AGGTCAAACC ATCCACTTCT
    AGCCAGCCTA TCTTGTCCAC TCCTGGTCCA GGAAAGCTAA CAGTAGGCAA CTGGTCTCTC
    ACTTGTCTGA AAGATGGTGA GATTGCAATC CACAACTCGG ACGGGCAGCA GGCTACAATA
    CTGAAGGAGG ATCTACCAGG CTTTGTGTTT GAGTCAAACA GGGGTACCAA GCACTCCTTC
    ACGGCAGAAA CATCACTAGG ATCTGAATTT GTAACTGGCT GGACAGGAAA GAGAGGCAGA
    AAGCTGAAGT CTAAGTTGGA GAAAACTAAA CAGAAGGTAC GGACAATGGC AAGAGATTTG
    TATGATGATC ATTTTAAGGC AGTGGAAAGC ATGCCCCGAG GAGTGGTAGT GACGCTCCGA
    AATATTGCAA CACAGCTGGA ATCTGCTTGG GAACTTCATA CAAACAGACA GTGTATTGAA
    GGAGAGAACA CATGGAGAGA TCTGATGAAA ACTGCCCTGG AAAACCTGAT TGTGCTACTG
    AAGGATGAGA ATACTATTTC CCCTTATGAG ATGTGCAGCA GTGGACTGGT GCAGGCTTTG
    CTTACGGTGC TCAATAATAA TGAAGACTGT GATATAAAAC AAGACTGTGG GCAGCTGGTG
    GAGAGACTTA ATGTATTTAA AACTGCCTTC AGCGAAAATG AAGATGATGA AAGCCGCCCT
    GCCGTTGCCC TCGTCCGGAA GTTGATTGCT GTGCTTGAAT CAATAGAACG GCTGCCCCTT
    CACCTGTATG ACACCCCTGG ATCTAGCTAT AACTTGCAGA TCCTGACCCG TAGGCTTCGG
    TTTCGGCTGG AGCGTGCACC TGGAGAAACG TCTCTAATCG ACCGTACCGG TCGGATGCTG
    AAAATGGAGC CACTGGCAAC TGTAGAGTCC TTGGAGCAGT ATTTACTGAA AATGGTGGCT
    AAGCAGTGGT ACGACTTTGA CCGTTCGTCC TTTGTGTTTG TGCGCAAATT GCGCGAGGGC
    CAGAGTTGTG TGTTCCGCCA CCAGCATGAC TTTGATGATA ATGGAATCAT GTACTGGATT
    GGCACCAATG CAAAGACAGC TTATGAGTGG GTCAACCCTG CTGCTTACGG ACTAGTAGTT
    GTTACATCTT CTGAAGGCCG CAATTTGCCA TATGGCCGCC TTGAGGATAT CCTGAGCCGT
    GATAGCTCTG CCCTTAATTG CCACACTAAT GATGATAAGA GTGCCTGGTT TGCCATTGAC
    CTTGGCTTGT GGGTTGTGCC TTCTGCCTAT ACACTGCGTC ATGCTCGTGG ATATGGCCGT
    TCCGCTCTTC GCAATTGGGT CTTCCAGGTG TCTAAAGATG GTCAGAACTG GACCACCCTC
    TACACCCACA TGGATGACTG CAGCCTGAAT GAACCTGGCT CCACAGCCAC ATGGCCTCTG
    GACCCTGCGA GAGAAGAAAA GCAGGGTTGG CGTCATGTCC GGATAAAGCA GACGGGAAAG
    AATGCCAGTG GCCAGACCCA CTACCTCTCA CTTTCTGGCT TTGAGCTTTA TGGCAATGTG
    ACTGGGGTGT GTGAAGACCA ACTTGGTAAA GCTGCCAAAG AGGCAGAAGC CAACTTAAGG
    CGCCAGAGGC GGCTGGTACG CTCTCAGGTG CTAAAATATA TGGTGCCTGG AGCCCGTGTT
    ATTCGGGGCA TTGATTGGAA GTGGCGAGAC CAAGATGGCA GTGCTCAAGG AGAAGGCACT
    GTTACAGGAG AACTGCACAA TGGCTGGATT GATGTCATAT GGGATGCCGG TGGCTCTAAC
    TCTTACCGCA TGGGCGCGGA AGGAAAATTT GACCTGAAGC TGGCCCCGGG TTATGACCCA
    GAGTCTGCGG TGTCACCCAA ACCTGTTTCA TCCACTGTTG CAGGAACACC GCCCTCGTGG
    AGCAGCCTGG TGAAAAACAA CTGTCCTGAC AAAGCCCCTC CCTCTTCCTC ATCCTCTTGT
    GTGGTCGTTG GAAGCGTGGC GGGCTCAGGC AGCCGCAAAG GAAGCAGCAG CTCGGTCTGT
    AGCGTGGCAA GCAGCAGCGA CGTAAGCCTA AGCTGTGCAA AGACAGAGAG GAGGGCAGAG
    GAGCAAGTCT CCGACATTCA CCATGATCCC ATCCTGCTTC TGTCCTCTAA CCAAGCAGCC
    TCGGGTTCCT CCACCTGCCC CCCTGGAGGA GAGACTGTGG GTGAAGGTGG AGATAGAAAG
    GCAGGAGAAG CACCAGCAAT CTCAATGGGT ATGGTGAGCA TTAGCTCCCC TGATGTCAGT
    TCCGTTTCCG AACTCAGCAA CAAGGAAGTG GCAGTTCCAC GACCACTGGG ATCTTCTGCC
    AGCAACAGAT TGTCTGTCAG TTCACTGTTG GCAGCTGGGG CACCCATGAG TTCTAGCGCC
    AGTGTACCAA ACTTGTCCTC CAGGGAAACG TCCAGTCTGG AGTCCTTTGT AAGGAGAGTG
    GCAAATATCG CCAGGACAAA TGCAACCAAC AACATGAACC TGAGTCGTAG CAGCAGTGAT
    AACAATACCA ACACTCTGGG GAGGAATGCA GTCAGCTCAG CCACTTCACC ACTTATGGGA
    GCTCAGAGCT TTCCCAACCT GACGACAACA GGAACCACAT CCACCGTTAC AATGTCTACA
    TCCAGTGTCA CCAGCAGTAA TGTGGCAACA GCCACTACAG GGTTGTCTGT AGGTCAGTCC
    CTCAGTAACA CCCTGACTAC AAGCCTAACC TCGACATCCA GTGAGAGTGA CACTGGGCAG
    GAGGCAGAAT ACTCCCTGTA TGACTTTCTT GATAGCTGCC GTGCCAGCAC TTTGCTTGCT
    GAACTTGATG ATGACGAGGA TCTCCCTGAG CCCGATGAGG AAGATGATGA AAACGAAGAT
    GATAATCAGG AAGAGCAAGA GTATGAAGAA GTTATGGAGG AGGAAGAGTA TGAGACCAAA
    GGTGGCCGAC GCAGGACCTG GGATGATGAT TATGTGCTGA AGAGGCAGTT TTCTGCTTTG
    GTCCCGGCTT TTGACCCACG CCCAGGGAGA ACTAATGTTC AGCAGACCAC TGATCTTGAA
    ATTCCTGCTC CAGGCACGCC CCACTCTGAG CTACTGGAGG AGGTAGAATG TGCCCCAGCT
    CCACGATTAG CACTCACCCT AAAGGTGACA GGCCTTGGGA GCGGGAGAGA GGTGGAGCTC
    CCCCTGAACA ATTTCCGTTC CACCATATTT TTCTATGTAC AGAGACTGCT GCAACTCTCC
    TGCAATGGAG CCATCAAAAC TGACAAGCTG AGAAGGATTT GGGAGCCCAC CTACACGATT
    ATGTACAGAG AGATGAAGGA TTCAGATAAG CAAAAAGAAT GTGGCAGACT GGGCTGTTGG
    TCTGTAGAGC ATGTAGAACA ATCCCTAGGC ACAGATGCCT TGCCAAAGAA TGACCTGATC
    ACTTACCTGC AGCGAAATGC AGATCCAGGG TTCTTGCGAC GCTGGAAACT GACAGGAACC
    AACAAAAGCA TTAGGAAAAA CCGCAACTGT TCCCAGCTTA TTGCTGCTTA CAAGGACTTC
    TGTGAGAATG GCTGCAAGTC TCTCTCAATG CCAGCTGCTC TTGCCACACT CCAGAGTGCT
    GATATCCTTA GTCATTCCCG AGAGCAGGCA CAAGCCAAAG CGGGCAGCAG CCAAAACTCC
    TGCGGGGTAG AAGATGTTTT GCAGCTCCTG CGAATTCTCT TTATTGTAGC CAGTGACCCA
    TATTCTGCCA GGACCCCACA GGAAGATGGA GAAGATATGC TCCTTTTCAG TGTTCCTCCA
    GAGGAGTTTA CTAGCAAGAA AATCACCACA AAGATTGTTC AGCAAATCGA GGAACCTTTA
    GCTTTAGCAA GTGGAGCTTT GCCAGACTGG TGTGAACAGC TGACCAGCAA ATGTCCCTTC
    TTAATACCCT TTGAAACCCG GCAGCTTTAC TTCACATGCA CAGCATTTGG TGCCTCAAGG
    GCTATTGTGT GGCTGCAGAA CAGGCGGGAA GCCACAGTTG AGAGGAGCAG AACAGCAAGT
    GCAGTGCGCA GAGACGACCC AGGGGAGTTC AGAGTGGGCC GTCTAAAACA CGAACGTGTG
    AAGGTCCCAC GAGGGGAGTC TTTGATGGAG TGGGCAGAGA ATGTTATGCA AATCCACGCA
    GACCGAAAAT CTGTGCTTGA GGTGGAGTTT CTGGGAGAAG AAGGAACAGG CTTGGGCCCT
    ACCCTTGAGT TTTATGCCCT GGTGGCAGCA GAGTTCCAGA AAACAGATTT GGGGATCTGG
    TTATGTGATG ATGACTTCCC AGATGATGAG TCTCGGCAGG TGGATCTAGG TGGTGGTTTG
    AAGCCCCCTG GTTACTATGT GCAGCGTTCC TGTGGCCTCT TCATTGCACC CTACCCACAG
    GATAGTGAGG AACTAGACCG AGTGACCAGA CTTTGCCATT TCCTTGGTGT TTTCCTTGCA
    AAGTGCATCC AGGACAATAG ATTAGTGGAC TTGCCCATTT CAAAGCCGTT TTTTAAGCTT
    ATGTGTATGG GTGACATTAA GAGCAACATG AGCAAGCTTT TGTATGCCTC TCGGGGAGAG
    GAGAGTGAGC ACTGCACGGA GAGCCAGTCG GAGGCCTCTA CTGAGGATGG GCATGATGCC
    CTTTCAGTAG GAAGCTTTGA GGAAGACTGC AAATCTGAGT TCATCCTAGA CCCACCCAAA
    CCAAAGCCAC CAGCCTGGTT TCAGGGTATT TTAACTTGGG AGGACTTTGA GCTAATAAAC
    CCACATCGTG CTCGCTTCCT TAGGGACATT AGGGAGTTGG CTGTAAAGAG GAGACAGATT
    CTGGGGAACC GTTGCTTATC TGAGGATGAG AAGAACACTC AACTTCAGGA ACTTATGCTA
    AAGAATCCTT CTGGATCTGG GCCTCCAGTC AGCATTGAGG ATTTGGGACT CAATTTCCAG
    TTCTGTCCAT CTTCCCGGGT TTATGGCTTC TCTGCAGTCG ATCTAAGGCC AAATGGAGAG
    GATGAGATGG TGACGATAGA CAACGCTGAG GAGTATGTTG ATCTTATGTT TGATTTCTGC
    ATGCAGACTG GTGTACAAAA GCAAATGGAA GCATTTAGAA GTGGCTTTAA TAAAGTGTTC
    CCTATGGAGA AACTGGGCTC CTTCAGCCCC GAGGAAGTCC AAATGATACT GTGTGGAAAC
    CAGTCTCCCT CTTGGTCCGC TGAGGACATC ATCAATTACA CAGAACCAAA GCTAGGATAT
    ACCCGAGAGA GTCCTGGGTT CCTTCGATTC GTGAGAGTCC TATGTGGCAT GTCGTCAGAT
    GAGAGGAAAG CGTTCCTGCA GTTTACAACG GGGTGCTCCA CATTGCCTCC AGGAGGACTG
    GCCAATCTGC ATCCGCGTCT CACAGTCGTG CGCAAGGTTG ATGCCACGGA TGCCAGCTAC
    CCATCTGTAA ATACATGTGT GCATTACCTG AAGCTGCCGG AGTATTCCTC CGAGGAAATC
    ATGAGAGACC GCCTGCTTGC TGCTACCATG GAGAAAGGAT TCCATCTCAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012823551.1
    CDS305..7978
    Translation

    Target ORF information:

    RefSeq Version XM_012968097.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 1 (hectd1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012968097.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGCTGACG TAGATCCAGA CACACTACTG GAATGGCTGC AGATGGGGCA GGGGGATGAG 
    AGAGACATGC AGCTCATTGC CTTGGAACAG CTCTGCATGC TGCTGCTCAT GTCGGATAAT
    GTGGATAGGT GTTTTGAAAC ATGCCCACCT CGCACCTTCC TGCCAGCCTT GTGTAAAATA
    TTCCTGGATG AAAGCGCCCC AGACAATGTG TTGGAAGTAA CAGCCCGTGC AATAACCTAT
    TACCTAGATG TGTCCGCCGA GTGCACGAGG CGTATTGTGG GAGTGGAAGG GGCTATCAAA
    GCTTTGTGCA ATAGGCTTGT GGTGGTGGAG CTCAACAACA GAACCAGTCG GGATTTGGCA
    GAACAGTGTG TCAAGGTGCT GGAGCTTATC TGTACACGTG AGTCTGGGGC TGTCTTTGAG
    GCAGGGGGCT TGAACTGCGT GTTGACATTC ATTCGGGACA GCGGACACCT AGTTCACAAG
    GATACACTGC ATTCTGCAAT GGCTGTGGTG TCCCGATTGT GTGGTAAGAT GGAGCCACAG
    GATGCATCAT TAGAAACGTG TGTGGAGTCT CTCTCCAGCC TGCTGAAGCA CGAAGACCAC
    CAGGTATCAG ATGGAGCTCT CCGCTGCTTT GCTTCCCTTG CTGACCGCTT TACACGCCGT
    GGTGTAGATC CAGCTCCTCT TGCACAGCAT GGGCTCACTG AGGAACTGCT GTCCCGAATG
    GCAGCAGCCG GAGGCACTGT ATCAGGTCCA TCTTCAGCCT GTAAAACAGG CCGTGGCACC
    AGTGGGGGAC CTTCCACCTC TGGAGACTCA AAAATCAGCA ATCAGGTCTC CACTATAGTC
    AGCCTGTTAT CAACACTATG CCGTGGTTCT CCAGTCGTCA CACATGATTT ACTGCGGGCA
    GAACTCTTAG ACTCCATGGA AAGTGCTCTT CAAGGTGACG AGCGTTGTGT GCTGGATACC
    ATGCGCCTTG TAGACCTTCT TTTGGTGCTG CTTTTTGAAG GCCGCAAAGC TCTGCCTAAG
    TCTAGTGCTG GGTCCACAGG TCGTATTCCT GGGCTGCGGC GGCTTGACAG CTCAGGAGAG
    CGATCTCACC GCCAACTGAT AGACTGCATC CGCAGCAAGG ACACAGATGC TCTCATAGAT
    GCCATTGATA CAGGTGCTTT TGAAGTAAAT TTTATGGATG ATGTTGGCCA GACTCTTCTG
    AATTGGGCCT CTGCTTTTGG CACACAGGAA ATGGTTGAAT TCCTGTGTGA GCGAGGTGCC
    GATGTAAATA GAGGGCAGAG GTCATCTTCA CTGCATTATG CTGCTTGCTT CGGAAGGCCT
    CAGGTGGCAA AGACCTTGCT TCGCCATGGA GCCAACCCAG ATTTAAGAGA TGAGGATGGA
    AAAACACCTT TGGATAAAGC CCGGGAGAGA GGACACAGCG AAGTGGTGGC CATTTTGCAG
    TCCCCAGGGG ACTGGATGTG CCCCGTAAAC AAAGGAGATG AAAAGAAAAA GAAAGACTCT
    AACCGGGAAG AGGAAGAATG CAATGAGCCA AAGGGAGACC CAGAAATGGC CCCCATCTAC
    CTAAAGAGGC TGCTGCCTGT CTTTGCACAG ACCTTTCAGC AAACCATGCT TCCCTCTATC
    AGGAAAGCAA GCTTAGCACT TATTCGGAAG ATGATCCATT TCTGCTCAGA AGCACTGCTT
    AAGGAAGTTT GTCACTCAGA CGCTGGACAC AATTTGCCCA CTGTTCTTGT TGAGATAACA
    GCCACTGTTT TAGACCAGGA GGACGATGAT GATGGTCACC TTCTGGCACT TCAAATCATT
    AGGGATTTGG TGGACAAAGG TGATGACCTT TTTCTTGATC AATTGGCCCG GCTTGGTGTT
    ATAAGCAAGG TTTCTACACT AGCTGGACCT ACATCAGATG ATGAGAATGA GGAGGATAGC
    AAGCCAGAGA AGGAGGATGA GCCACAGGAA GATGCCAAGG AGTTGCAACA GGGCCGGCCC
    TACCATTGGC GGGACTGGTC TGTTATTCGC GGTAGGGACT GTTTGTACAT CTGGAGTGAT
    GCTGCAGCTC TGGAGCTCTC TAATGGTAGC AATGGCTGGT TCCGCTTTAT CCTAGATGGG
    AAACTGGCAA CCATGTATTC AAGTGGAAGC CCAGAGGGGG GTTCTGATAG CTCAGAAAGT
    AGAAGTGAAT TTTTAGAAAA GTTACAGCGG GCCCGCAGCC AGGTCAAACC ATCCACTTCT
    AGCCAGCCTA TCTTGTCCAC TCCTGGTCCA GGAAAGCTAA CAGTAGGCAA CTGGTCTCTC
    ACTTGTCTGA AAGATGGTGA GATTGCAATC CACAACTCGG ACGGGCAGCA GGCTACAATA
    CTGAAGGAGG ATCTACCAGG CTTTGTGTTT GAGTCAAACA GGGGTACCAA GCACTCCTTC
    ACGGCAGAAA CATCACTAGG ATCTGAATTT GTAACTGGCT GGACAGGAAA GAGAGGCAGA
    AAGCTGAAGT CTAAGTTGGA GAAAACTAAA CAGAAGGTAC GGACAATGGC AAGAGATTTG
    TATGATGATC ATTTTAAGGC AGTGGAAAGC ATGCCCCGAG GAGTGGTAGT GACGCTCCGA
    AATATTGCAA CACAGCTGGA ATCTGCTTGG GAACTTCATA CAAACAGACA GTGTATTGAA
    GGAGAGAACA CATGGAGAGA TCTGATGAAA ACTGCCCTGG AAAACCTGAT TGTGCTACTG
    AAGGATGAGA ATACTATTTC CCCTTATGAG ATGTGCAGCA GTGGACTGGT GCAGGCTTTG
    CTTACGGTGC TCAATAATAA TGAAGACTGT GATATAAAAC AAGACTGTGG GCAGCTGGTG
    GAGAGACTTA ATGTATTTAA AACTGCCTTC AGCGAAAATG AAGATGATGA AAGCCGCCCT
    GCCGTTGCCC TCGTCCGGAA GTTGATTGCT GTGCTTGAAT CAATAGAACG GCTGCCCCTT
    CACCTGTATG ACACCCCTGG ATCTAGCTAT AACTTGCAGA TCCTGACCCG TAGGCTTCGG
    TTTCGGCTGG AGCGTGCACC TGGAGAAACG TCTCTAATCG ACCGTACCGG TCGGATGCTG
    AAAATGGAGC CACTGGCAAC TGTAGAGTCC TTGGAGCAGT ATTTACTGAA AATGGTGGCT
    AAGCAGTGGT ACGACTTTGA CCGTTCGTCC TTTGTGTTTG TGCGCAAATT GCGCGAGGGC
    CAGAGTTGTG TGTTCCGCCA CCAGCATGAC TTTGATGATA ATGGAATCAT GTACTGGATT
    GGCACCAATG CAAAGACAGC TTATGAGTGG GTCAACCCTG CTGCTTACGG ACTAGTAGTT
    GTTACATCTT CTGAAGGCCG CAATTTGCCA TATGGCCGCC TTGAGGATAT CCTGAGCCGT
    GATAGCTCTG CCCTTAATTG CCACACTAAT GATGATAAGA GTGCCTGGTT TGCCATTGAC
    CTTGGCTTGT GGGTTGTGCC TTCTGCCTAT ACACTGCGTC ATGCTCGTGG ATATGGCCGT
    TCCGCTCTTC GCAATTGGGT CTTCCAGGTG TCTAAAGATG GTCAGAACTG GACCACCCTC
    TACACCCACA TGGATGACTG CAGCCTGAAT GAACCTGGCT CCACAGCCAC ATGGCCTCTG
    GACCCTGCGA GAGAAGAAAA GCAGGGTTGG CGTCATGTCC GGATAAAGCA GACGGGAAAG
    AATGCCAGTG GCCAGACCCA CTACCTCTCA CTTTCTGGCT TTGAGCTTTA TGGCAATGTG
    ACTGGGGTGT GTGAAGACCA ACTTGGTAAA GCTGCCAAAG AGGCAGAAGC CAACTTAAGG
    CGCCAGAGGC GGCTGGTACG CTCTCAGGTG CTAAAATATA TGGTGCCTGG AGCCCGTGTT
    ATTCGGGGCA TTGATTGGAA GTGGCGAGAC CAAGATGGCA GTGCTCAAGG AGAAGGCACT
    GTTACAGGAG AACTGCACAA TGGCTGGATT GATGTCATAT GGGATGCCGG TGGCTCTAAC
    TCTTACCGCA TGGGCGCGGA AGGAAAATTT GACCTGAAGC TGGCCCCGGG TTATGACCCA
    GAGTCTGCGG TGTCACCCAA ACCTGTTTCA TCCACTGTTG CAGGAACACC GCCCTCGTGG
    AGCAGCCTGG TGAAAAACAA CTGTCCTGAC AAAGCCCCTC CCTCTTCCTC ATCCTCTTGT
    GTGGTCGTTG GAAGCGTGGC GGGCTCAGGC AGCCGCAAAG GAAGCAGCAG CTCGGTCTGT
    AGCGTGGCAA GCAGCAGCGA CGTAAGCCTA AGCTGTGCAA AGACAGAGAG GAGGGCAGAG
    GAGCAAGTCT CCGACATTCA CCATGATCCC ATCCTGCTTC TGTCCTCTAA CCAAGCAGCC
    TCGGGTTCCT CCACCTGCCC CCCTGGAGGA GAGACTGTGG GTGAAGGTGG AGATAGAAAG
    GCAGGAGAAG CACCAGCAAT CTCAATGGGT ATGGTGAGCA TTAGCTCCCC TGATGTCAGT
    TCCGTTTCCG AACTCAGCAA CAAGGAAGTG GCAGTTCCAC GACCACTGGG ATCTTCTGCC
    AGCAACAGAT TGTCTGTCAG TTCACTGTTG GCAGCTGGGG CACCCATGAG TTCTAGCGCC
    AGTGTACCAA ACTTGTCCTC CAGGGAAACG TCCAGTCTGG AGTCCTTTGT AAGGAGAGTG
    GCAAATATCG CCAGGACAAA TGCAACCAAC AACATGAACC TGAGTCGTAG CAGCAGTGAT
    AACAATACCA ACACTCTGGG GAGGAATGCA GTCAGCTCAG CCACTTCACC ACTTATGGGA
    GCTCAGAGCT TTCCCAACCT GACGACAACA GGAACCACAT CCACCGTTAC AATGTCTACA
    TCCAGTGTCA CCAGCAGTAA TGTGGCAACA GCCACTACAG GGTTGTCTGT AGGTCAGTCC
    CTCAGTAACA CCCTGACTAC AAGCCTAACC TCGACATCCA GTGAGAGTGA CACTGGGCAG
    GAGGCAGAAT ACTCCCTGTA TGACTTTCTT GATAGCTGCC GTGCCAGCAC TTTGCTTGCT
    GAACTTGATG ATGACGAGGA TCTCCCTGAG CCCGATGAGG AAGATGATGA AAACGAAGAT
    GATAATCAGG AAGAGCAAGA GTATGAAGAA GTTATGGAGG AGGAAGAGTA TGAGACCAAA
    GGTGGCCGAC GCAGGACCTG GGATGATGAT TATGTGCTGA AGAGGCAGTT TTCTGCTTTG
    GTCCCGGCTT TTGACCCACG CCCAGGGAGA ACTAATGTTC AGCAGACCAC TGATCTTGAA
    ATTCCTGCTC CAGGCACGCC CCACTCTGAG CTACTGGAGG AGGTAGAATG TGCCCCAGCT
    CCACGATTAG CACTCACCCT AAAGGTGACA GGCCTTGGGA GCGGGAGAGA GGTGGAGCTC
    CCCCTGAACA ATTTCCGTTC CACCATATTT TTCTATGTAC AGAGACTGCT GCAACTCTCC
    TGCAATGGAG CCATCAAAAC TGACAAGCTG AGAAGGATTT GGGAGCCCAC CTACACGATT
    ATGTACAGAG AGATGAAGGA TTCAGATAAG CAAAAAGAAT GTGGCAGACT GGGCTGTTGG
    TCTGTAGAGC ATGTAGAACA ATCCCTAGGC ACAGATGCCT TGCCAAAGAA TGACCTGATC
    ACTTACCTGC AGCGAAATGC AGATCCAGGG TTCTTGCGAC GCTGGAAACT GACAGGAACC
    AACAAAAGCA TTAGGAAAAA CCGCAACTGT TCCCAGCTTA TTGCTGCTTA CAAGGACTTC
    TGTGAGAATG GCTGCAAGTC TCTCTCAATG CCAGCTGCTC TTGCCACACT CCAGAGTGCT
    GATATCCTTA GTCATTCCCG AGAGCAGGCA CAAGCCAAAG CGGGCAGCAG CCAAAACTCC
    TGCGGGGTAG AAGATGTTTT GCAGCTCCTG CGAATTCTCT TTATTGTAGC CAGTGACCCA
    TATTCTGCCA GGACCCCACA GGAAGATGGA GAAGATATGC TCCTTTTCAG TGTTCCTCCA
    GAGGAGTTTA CTAGCAAGAA AATCACCACA AAGATTGTTC AGCAAATCGA GGAACCTTTA
    GCTTTAGCAA GTGGAGCTTT GCCAGACTGG TGTGAACAGC TGACCAGCAA ATGTCCCTTC
    TTAATACCCT TTGAAACCCG GCAGCTTTAC TTCACATGCA CAGCATTTGG TGCCTCAAGG
    GCTATTGTGT GGCTGCAGAA CAGGCGGGAA GCCACAGTTG AGAGGAGCAG AACAGCAAGT
    GCAGTGCGCA GAGACGACCC AGGGGAGTTC AGAGTGGGCC GTCTAAAACA CGAACGTGTG
    AAGGTCCCAC GAGGGGAGTC TTTGATGGAG TGGGCAGAGA ATGTTATGCA AATCCACGCA
    GACCGAAAAT CTGTGCTTGA GGTGGAGTTT CTGGGAGAAG AAGGAACAGG CTTGGGCCCT
    ACCCTTGAGT TTTATGCCCT GGTGGCAGCA GAGTTCCAGA AAACAGATTT GGGGATCTGG
    TTATGTGATG ATGACTTCCC AGATGATGAG TCTCGGCAGG TGGATCTAGG TGGTGGTTTG
    AAGCCCCCTG GTTACTATGT GCAGCGTTCC TGTGGCCTCT TCATTGCACC CTACCCACAG
    GATAGTGAGG AACTAGACCG AGTGACCAGA CTTTGCCATT TCCTTGGTGT TTTCCTTGCA
    AAGTGCATCC AGGACAATAG ATTAGTGGAC TTGCCCATTT CAAAGCCGTT TTTTAAGCTT
    ATGTGTATGG GTGACATTAA GAGCAACATG AGCAAGCTTT TGTATGCCTC TCGGGGAGAG
    GAGAGTGAGC ACTGCACGGA GAGCCAGTCG GAGGCCTCTA CTGAGGATGG GCATGATGCC
    CTTTCAGTAG GAAGCTTTGA GGAAGACTGC AAATCTGAGT TCATCCTAGA CCCACCCAAA
    CCAAAGCCAC CAGCCTGGTT TCAGGGTATT TTAACTTGGG AGGACTTTGA GCTAATAAAC
    CCACATCGTG CTCGCTTCCT TAGGGACATT AGGGAGTTGG CTGTAAAGAG GAGACAGATT
    CTGGGGAACC GTTGCTTATC TGAGGATGAG AAGAACACTC AACTTCAGGA ACTTATGCTA
    AAGAATCCTT CTGGATCTGG GCCTCCAGTC AGCATTGAGG ATTTGGGACT CAATTTCCAG
    TTCTGTCCAT CTTCCCGGGT TTATGGCTTC TCTGCAGTCG ATCTAAGGCC AAATGGAGAG
    GATGAGATGG TGACGATAGA CAACGCTGAG GAGTATGTTG ATCTTATGTT TGATTTCTGC
    ATGCAGACTG GTGTACAAAA GCAAATGGAA GCATTTAGAA GTGGCTTTAA TAAAGTGTTC
    CCTATGGAGA AACTGGGCTC CTTCAGCCCC GAGGAAGTCC AAATGATACT GTGTGGAAAC
    CAGTCTCCCT CTTGGTCCGC TGAGGACATC ATCAATTACA CAGAACCAAA GCTAGGATAT
    ACCCGAGAGA GTCCTGGGTT CCTTCGATTC GTGAGAGTCC TATGTGGCAT GTCGTCAGAT
    GAGAGGAAAG CGTTCCTGCA GTTTACAACG GGGTGCTCCA CATTGCCTCC AGGAGGACTG
    GCCAATCTGC ATCCGCGTCT CACAGTCGTG CGCAAGGTTG ATGCCACGGA TGCCAGCTAC
    CCATCTGTAA ATACATGTGT GCATTACCTG AAGCTGCCGG AGTATTCCTC CGAGGAAATC
    ATGAGAGACC GCCTGCTTGC TGCTACCATG GAGAAAGGAT TCCATCTCAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa22713
    Clone ID Related Accession (Same CDS sequence) NM_001137613.1
    Accession Version NM_001137613.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7602bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase HECTD1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC168474.1. ##Evidence-Data-START## Transcript exon combination :: BC168474.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGGCTGACG TAGATCCAGA CACACTACTG GAATGGCTGC AGATGGGGCA GGGGGACGAG 
    AGAGACATGC AGCTCATTGC CTTGGAACAG CTCTGCATGC TGCTGCTCAT GTCGGATAAT
    GTGGATAGGT GTTTTGAAAC ATGCCCACCT CGCACCTTCC TGCCAGCCTT GTGTAAAATA
    TTCCTGGATG AAAGCGCCCC AGACAATGTG TTGGAAGTAA CAGCCCGTGC AATAACCTAT
    TACCTAGATG TGTCCGCCGA GTGCACGAGG CGTATTGTGG GAGTGGAAGG GGCTATCAAA
    GCTTTGTGCA ATAGGCTTGT GGTGGTGGAG CTCAACAACA GAACCAGTCG GGATTTGGCA
    GAACAGTGTG TCAAGGTGCT GGAGCTTATC TGTACACGTG AGTCTGGGGC TGTCTTTGAG
    GCAGGGGGCT TGAACTGCGT GTTGACATTC ATTCGGGACA GCGGACACCT AGTTCACAAG
    GATACGCTGC ATTCTGCAAT GGCTGTGGTG TCCCGACTGT GTGGTAAGAT GGAGCCACAG
    GATGCATCAT TAGAAACGTG TGTGGAGTCT CTCTCCAGCC TGCTGAAGCA CGAAGACCAT
    CAGGTATCAG ATGGAGCTCT CCGCTGCTTT GCTTCCCTTG CTGACCGCTT TACACGCCGT
    GGTGTAGATC CAGCTCCTCT TGCACAGCAT GGGCTCACTG AGGAACTGCT GTCCCGAATG
    GCAGCAGCCG GAGGCACTGT ATCAGGTCCA TCTTCAGCCT GTAAAACAGG CCGTGGCACC
    AGTGGGGGAC CTTCCACCTC TGGAGACTCA AAAATCAGCA ATCAGGTCTC CACTATAGTC
    AGCCTGTTAT CAACACTATG CCGTGGTTCT CCAGTCGTCA CACATGATTT ACTGCGGGCA
    GAACTCTTAG ACTCCATGGA AAGTGCTCTT CAAGGTGACG AGCGTTGTGT GCTGGATACC
    ATGCGCCTTG TAGACCTTCT TTTGGTGCTG CTTTTTGAAG GCCGCAAAGC TCTGCCTAAG
    TCTAGTGCTG GGTCCACAGG TCGTATTCCT GGGCTGCGGC GGCTTGACAG CTCAGGAGAG
    CGATCTCACC GCCAACTGAT AGACTGCATC CGCAGCAAGG ACACAGATGC TCTCATAGAT
    GCCATTGATA CAGGTGCTTT TGAAGTAAAT TTTATGGATG ATGTTGGCCA GACTCTTCTG
    AATTGGGCCT CTGCTTTTGG CACACAGGAA ATGGTTGAAT TCCTGTGTGA GCGAGGTGCC
    GATGTAAATA GAGGGCAGAG GTCATCTTCA CTGCATTATG CTGCTTGCTT CGGAAGGCCT
    CAGGTGGCAA AGACGTTGCT TCGCCATGGA GCCAACCCAG ATTTAAGAGA TGAGGATGGA
    AAAACACCTT TGGATAAAGC CCGGGAGAGA GGACACAGCG AAGTGGTGGC CATTTTGCAG
    TCCCCAGGGG ACTGGATGTG CCCCGTAAAC AAAGGAGATG AAAAGAAAAA GAAAGACTCT
    AACCGGGAAG AGGAAGAATG CAATGAGCCA AAGGGAGACC CAGAAATGGC CCCCATCTAC
    CTAAAGAGGC TGCTGCCTGT CTTTGCACAG ACCTTTCAGC AAACCATGCT TCCCTCTATC
    AGGAAAGCAA GCTTAGCACT TATTCGGAAG ATGATCCATT TCTGCTCAGA AGCACTGCTT
    AAGGAAGTTT GTCACTCAGA CGCTGGACAC AATTTGCCCA CTGTTCTTGT TGAGATAACA
    GCCACTGTTT TAGACCAGGA GGACGATGAT GATGGTCACC TTCTGGCACT TCAAATCATT
    AGGGATTTGG TGGACAAAGG TGATGACCTT TTTCTTGATC AATTGGCCCG GCTTGGTGTT
    ATAAGCAAGG TTTCTACACT AGCTGGACCT ACATCAGATG ATGAGAATGA GGAGGATAGC
    AAGCCAGAGA AGGTGAGGAT ACACCTATTT CCTCTGTACA GTGCCCACAA AGGAACACAA
    GCAGCTGCAT CACAACTTAA AGAGGATGAG CCACAGGAAG ATGCCAAGGA GTTGCAACAG
    GGCCGGCCCT ACCATTGGCG GGACTGGTCT GTTATTCGCG GTAGGGACTG TTTGTACATC
    TGGAGTGATG CTGCAGCTCT GGAGCTCTCT AATGGTAGCA ATGGCTGGTT CCGCTTTATC
    CTAGATGGGA AACTGGCAAC CATGTATTCA AGTGGAAGCC CAGAGGGGGG TTCTGATAGC
    TCAGAAAGTA GAAGTGAATT TTTAGAAAAG TTACAGCGGG CCCGCAGCCA GGTCAAACCA
    TCCACTTCTA GCCAGCCTAT CTTGTCCACT CCTGGTCCAG GAAAGCTAAC AGTAGGCAAC
    TGGTCTCTCA CTTGTCTGAA AGATGGTGAG ATTGCAATCC ACAACTCGGA CGGGCAGCAG
    GCTACAATAC TGAAGGAGGA TCTACCAGGC TTTGTGTTTG AGTCAAACAG GGGTACCAAG
    CACTCCTTCA CGGCAGAAAC ATCACTAGGA TCTGAATTTG TAACTGGCTG GACAGGAAAG
    AGAGGCAGAA AGCTGAAGTC TAAGTTGGAG AAAACTAAAC AGAAGGTACG GACAATGGCA
    AGAGATTTGT ATGATGATCA TTTTAAGGCA GTGGAAAGCA TGCCCCGAGG AGTGGTAGTG
    ACGCTTCGAA ATATTGCAAC ACAGCTGGAA TCTGCTTGGG AACTTCATAC AAACAGACAG
    TGTATTGAAG GAGAGAACAC ATGGAGAGAT CTGATGAAAA CTGCCCTGGA AAACCTGATT
    GTGCTACTGA AGGATGAGAA TACTATTTCC CCTTATGAGA TGTGCAGCAG TGGACTGGTG
    CAGGCTTTGC TTACGGTGCT CAATAATAAT GAAGACTGTG ATATAAAACA AGACTGTGGG
    CAGCTGGTGG AGAGACTTAA TGTATTTAAA ACTGCCTTCA GCGAAAATGA AGATGATGAA
    AGCCGCCCTG CCGTTGCCCT CGTCCGGAAG TTGATTGCTG TGCTTGAATC AATAGAACGG
    CTGCCCCTTC ACCTGTATGA CACCCCTGGA TCTAGCTATA ACTTGCAGAT CCTGACCCGT
    AGGCTTCGGT TTCGGCTGGA GCGTGCACCT GGAGAAACGT CTCTAATCGA CCGTACCGGT
    CGGATGCTGA AAATGGAGCC ACTGGCAACT GTAGAGTCCT TGGAGCAGTA TTTACTGAAA
    ATGGTGGCTA AGCAGTGGTA CGACTTTGAC CGTTCGTCCT TTGTGTTTGT GCGCAAATTG
    CGCGAGGGCC AGAGTTGTGT GTTCCGCCAC CAGCATGACT TTGATGATAA TGGAATCATG
    TACTGGATTG GCACCAATGC AAAGACAGCT TATGAGTGGG TCAACCCTGC TGCTTACGGA
    CTAGTAGTTG TTACATCTTC TGAAGGCCGC AATTTGCCAT ATGGCCGCCT TGAGGATATC
    CTGAGCCGTG ATAGCTCTGC CCTTAATTGC CACACTAATG ATGATAAGAG TGCCTGGTTT
    GCCATTGACC TTGGCTTGTG GGTTGTGCCT TCTGCCTATA CACTGCGTCA TGCTCGTGGA
    TATGGCCGTT CCGCTCTTCG CAATTGGGTC TTCCAGGTGT CTAAAGATGG TCAGAACTGG
    ACCACCCTCT ACACCCACAT GGATGACTGC AGCCTGAATG AACCTGGCTC CACAGCCACA
    TGGCCTCTGG ACCCTGCGAG AGAAGAAAAG CAGGGTTGGC GTCATGTCCG GATAAAGCAG
    ACGGGAAAGA ATGCCAGTGG CCAGACCCAC TACCTCTCAC TTTCTGGCTT TGAGCTTTAT
    GGCAATGTGA CTGGGGTGTG TGAAGACCAA CTTGGTAAAG CTGCCAAAGA GGCAGAAGCC
    AACTTAAGGC GCCAGAGGCG GCTGGTACGC TCTCAGGTGC TAAAATATAT GGTGCCTGGA
    GCCCGTGTTA TTCGGGGCAT TGATTGGAAG TGGCGAGACC AAGATGGCAG TGCTCAAGGA
    GAAGGCACTG TTACAGGAGA ACTGCACAAT GGAACACCGC CCTCGTGGAG CAGCCTGGTG
    AAAAACAACT GTCCTGACAA AGCCCCTCCC TCTTCCTCAT CCTCTTGTGT GGTCGTTGGA
    AGCGTGGCGG GCTCAGGCAG CCGCAAAGGA AGCAGCAGCT CGGTCTGTAG CGTGGCAAGC
    AGCAGCGACG TAAGCCTAAG CTGTGCAAAG ACAGAGAGGA GGGCAGAGGA GCAAGTCTCC
    GACATTCACC ATGATCCCAT CCTGCTTCTG TCCTCTAACC AAGCAGCCTC GGGTTCCTCC
    ACCTGCCCCC CTGGAGGAGA GACTGTGGGT GAAGGTGGAG ATAGAAAGGC AGGAGAAGCA
    CCAGCAATCT CAATGGGTAT GGTGAGCATT AGCTCCCCTG ATGTCAGTTC CGTTTCCGAA
    CTCAGCAACA AGGAAGTGGC AGTTCCACGA CCACTGGGAT CTTCTGCCAG CAACAGATTG
    TCTGTCAGTT CACTGTTGGC AGCTGGGGCA CCCATGAGTT CTAGCGCCAG TGTACCAAAC
    TTGTCCTCCA GGGAAACGTC CAGTCTGGAG TCCTTTGTAA GGAGAGTGGC AAATATCGCC
    AGGACAAATG CAACCAACAA CATGAACCTG AGTCGTAGCA GCAGTGATAA CAATACCAAC
    ACTCTGGGGA GGAATGCAGT CAGCTCAGCC ACTTCACCAC TTATGGGAGC TCAGAGCTTT
    CCCAACCTGA CGACAACAGG AACCACATCC ACTGTTACAA TGTCTACATC CAGTGTCACC
    AGCAGTAATG TGGCAACAGC CACTACAGGG TTGTCTGTAG GTCAGTCCCT CAGTAACACC
    CTGACTACAA GCCTAACCTC GACATCCAGT GAGAGTGACA CTGGGCAGGA GGCAGAATAC
    TCCCTGTATG ACTTTCTTGA TAGCTGCCGT GCCAGCACTT TGCTTGCTGA ACTTGATGAT
    GACGAGGATC TCCCTGAGCC CGATGAGGAA GATGATGAAA ACGAAGATGA TAATCAGGAA
    GAGCAAGAGT ATGAAGAAGT TATGGAGGAG GAAGAGTATG AGACCAAAGG TGGCCGACGC
    AGGACCTGGG ATGATGATTA TGTGCTGAAG AGGCAGTTTT CTGCTTTGGT CCCGGCTTTT
    GACCCACGCC CAGGGAGAAC TAATGTTCAG CAGACCACTG ATCTTGAAAT TCCTGCTCCA
    GGCACGCCCC ACTCTGAGCT ACTGGAGGAG GTAGAATGTG CCCCAGCTCC ACGATTAGCA
    CTCACCCTAA AGGTGACAGG CCTTGGGAGC GGGAGAGAGG TGGAGCTCCC CCTGAACAAT
    TTCCGTTCCA CCATATTTTT CTATGTACAG AGACTGCTGC AACTCTCCTG CAATGGAGCC
    ATCAAAACTG ACAAGCTGAG AAGGATTTGG GAGCCCACCT ACACGATTAT GTACAGAGAG
    ATGAAGGATT CAGATAAGCA AAAAGAATGT GGCAGACTGG GCTGTTGGTC TGTAGAGCAT
    GTGGAACAAT CCCTAGGCAC AGATGCCTTG CCAAAGAATG ACCTGATCAC TTACCTGCAG
    CGAAATGCAG ATCCAGGGTT CTTGCGACGC TGGAAACTGA CAGGAACCAA CAAAAGCATT
    AGGAAAAACC GCAACTGTTC CCAGCTTATT GCTGCTTACA AGGACTTCTG TGAGAATGGC
    TGCAAGTCTC TCTCAATGCC AGCTGCTCTT GCCACACTCC AGAGTGCTGA TATCCTTAGT
    CATTCCCGAG AGCAGGCACA AGCCAAAGCG GGCAGCAGCC AAAACTCCTG CGGGGTAGAA
    GATGTTTTGC AGCTCCTGCG AATTCTCTTT ATTGTAGCCA GTGACCCATA TTCTGCCAGG
    ACCCCACAGG AAGATGGAGA AGATATGCTC CTTTTCAGTG TTCCTCCAGA GGAGTTTACT
    AGCAAGAAAA TCACCACAAA GATTGTTCAG CAAATCGAGG AACCTTTAGC TTTAGCAAGT
    GGAGCTTTGC CAGACTGGTG TGAACAGCTG ACCAGCAAAT GTCCCTTCTT AATACCCTTT
    GAAACCCGGC AGCTTTACTT CACATGCACA GCATTTGGTG CCTCAAGGGC TATTGTGTGG
    CTGCAGAACA GGCGGGAAGC CACAGTTGAG AGGAGCAGAA CAGCAAGTGC AGTGCGCAGA
    GACGACCCAG GGGAGTTCAG AGTGGGCCGT CTAAAACACG AACGTGTGAA GGTCCCACGA
    GGGGAGTCTT TGATGGAGTG GGCAGAGAAT GTTATGCAAA TCCACGCAGA CCGAAAATCT
    GTGCTTGAGG TGGAGTTTCT GGGAGAAGAA GGAACAGGCT TGGGCCCTAC CCTTGAGTTT
    TATGCCCTGG TGGCAGCAGA GTTCCAGAAA ACAGATTTGG GGATCTGGTT ATGTGATGAT
    GACTTCCCAG ATGATGAGTC TCGGCAGGTG GATCTAGGTG GTGGTTTGAA GCCCCCTGGT
    TACTATGTGC AGCGTTCCTG TGGCCTCTTC ATTGCACCCT ACCCACAGGA TAGTGAGGAA
    CTAGACCGAG TGACCAGACT TTGCCATTTC CTTGGTGTTT TCCTTGCAAA GTGCATCCAG
    GACAATAGAT TAGTGGACTT GCCCATTTCA AAGCCGTTTT TTAAGCTTAT GTGTATGGGT
    GACATTAAGA GCAACATGAG CAAGCTTTTG TATGCCTCTC GGGGAGAGGA GAGTGAGCAC
    TGCACGGAGA GCCAGTCGGA GGCCTCTACT GAGGATGGGC ATGATGCCCT TTCAGTAGGA
    AGCTTTGAGG AAGACTGCAA ATCTGAGTTC ATCCTAGACC CACCCAAACC AAAGCCACCA
    GCCTGGTTTC AGGGTATTTT AACTTGGGAG GACTTTGAGC TAATAAACCC ACATCGTGCT
    CGCTTCCTTA GGGACATTAG GGAGTTGGCT GTAAAGAGGA GACAGATTCT GGGGAACCGT
    TGCTTATCTG AGGATGAGAA GAACACTCAA CTTCAGGAAC TTATGCTAAA GAATCCTTCT
    GGATCTGGGC CTCCAGTCAG CATTGAGGAT TTGGGACTCA ATTTCCAGTT CTGTCCATCT
    TCCCGGGTTT ATGGCTTCTC TGCAGTCGAT CTAAGGCCAA ATGGAGAGGA TGAGATGGTG
    ACGATAGACA ACGCTGAGGA GTATGTTGAT CTTATGTTTG ATTTCTGCAT GCAGACTGGT
    GTACAAAAGC AAATGGAAGC ATTTAGAAGT GGCTTTAATA AAGTGTTCCC TATGGAGAAA
    CTGGGCTCCT TCAGCCCCGA GGAAGTCCAA ATGATACTGT GTGGAAACCA GTCTCCCTCT
    TGGTCCGCTG AGGACATCAT CAATTACACA GAACCAAAGC TAGGATATAC CCGAGAGAGT
    CCTGGGTTCC TTCGATTCGT GAGAGTCCTA TGTGGCATGT CGTCAGATGA GAGGAAAGCG
    TTCCTGCAGT TTACAACGGG GTGCTCCACA TTGCCTCCAG GAGGACTGGC CAATCTGCAT
    CCGCGTCTCA CAGTCGTGCG CAAGGTTGAT GCCACGGATG CCAGCTACCC ATCTGTAAAT
    ACATGTGTGC ATTACCTGAA GCTGCCGGAG TATTCCTCCG AGGAAATCAT GAGAGACCGC
    CTGCTTGCTG CTACCATGGA GAAAGGATTC CATCTCAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001131085.1
    CDS181..7782
    Translation

    Target ORF information:

    RefSeq Version NM_001137613.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 1 (hectd1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001137613.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGGCTGACG TAGATCCAGA CACACTACTG GAATGGCTGC AGATGGGGCA GGGGGACGAG 
    AGAGACATGC AGCTCATTGC CTTGGAACAG CTCTGCATGC TGCTGCTCAT GTCGGATAAT
    GTGGATAGGT GTTTTGAAAC ATGCCCACCT CGCACCTTCC TGCCAGCCTT GTGTAAAATA
    TTCCTGGATG AAAGCGCCCC AGACAATGTG TTGGAAGTAA CAGCCCGTGC AATAACCTAT
    TACCTAGATG TGTCCGCCGA GTGCACGAGG CGTATTGTGG GAGTGGAAGG GGCTATCAAA
    GCTTTGTGCA ATAGGCTTGT GGTGGTGGAG CTCAACAACA GAACCAGTCG GGATTTGGCA
    GAACAGTGTG TCAAGGTGCT GGAGCTTATC TGTACACGTG AGTCTGGGGC TGTCTTTGAG
    GCAGGGGGCT TGAACTGCGT GTTGACATTC ATTCGGGACA GCGGACACCT AGTTCACAAG
    GATACGCTGC ATTCTGCAAT GGCTGTGGTG TCCCGACTGT GTGGTAAGAT GGAGCCACAG
    GATGCATCAT TAGAAACGTG TGTGGAGTCT CTCTCCAGCC TGCTGAAGCA CGAAGACCAT
    CAGGTATCAG ATGGAGCTCT CCGCTGCTTT GCTTCCCTTG CTGACCGCTT TACACGCCGT
    GGTGTAGATC CAGCTCCTCT TGCACAGCAT GGGCTCACTG AGGAACTGCT GTCCCGAATG
    GCAGCAGCCG GAGGCACTGT ATCAGGTCCA TCTTCAGCCT GTAAAACAGG CCGTGGCACC
    AGTGGGGGAC CTTCCACCTC TGGAGACTCA AAAATCAGCA ATCAGGTCTC CACTATAGTC
    AGCCTGTTAT CAACACTATG CCGTGGTTCT CCAGTCGTCA CACATGATTT ACTGCGGGCA
    GAACTCTTAG ACTCCATGGA AAGTGCTCTT CAAGGTGACG AGCGTTGTGT GCTGGATACC
    ATGCGCCTTG TAGACCTTCT TTTGGTGCTG CTTTTTGAAG GCCGCAAAGC TCTGCCTAAG
    TCTAGTGCTG GGTCCACAGG TCGTATTCCT GGGCTGCGGC GGCTTGACAG CTCAGGAGAG
    CGATCTCACC GCCAACTGAT AGACTGCATC CGCAGCAAGG ACACAGATGC TCTCATAGAT
    GCCATTGATA CAGGTGCTTT TGAAGTAAAT TTTATGGATG ATGTTGGCCA GACTCTTCTG
    AATTGGGCCT CTGCTTTTGG CACACAGGAA ATGGTTGAAT TCCTGTGTGA GCGAGGTGCC
    GATGTAAATA GAGGGCAGAG GTCATCTTCA CTGCATTATG CTGCTTGCTT CGGAAGGCCT
    CAGGTGGCAA AGACGTTGCT TCGCCATGGA GCCAACCCAG ATTTAAGAGA TGAGGATGGA
    AAAACACCTT TGGATAAAGC CCGGGAGAGA GGACACAGCG AAGTGGTGGC CATTTTGCAG
    TCCCCAGGGG ACTGGATGTG CCCCGTAAAC AAAGGAGATG AAAAGAAAAA GAAAGACTCT
    AACCGGGAAG AGGAAGAATG CAATGAGCCA AAGGGAGACC CAGAAATGGC CCCCATCTAC
    CTAAAGAGGC TGCTGCCTGT CTTTGCACAG ACCTTTCAGC AAACCATGCT TCCCTCTATC
    AGGAAAGCAA GCTTAGCACT TATTCGGAAG ATGATCCATT TCTGCTCAGA AGCACTGCTT
    AAGGAAGTTT GTCACTCAGA CGCTGGACAC AATTTGCCCA CTGTTCTTGT TGAGATAACA
    GCCACTGTTT TAGACCAGGA GGACGATGAT GATGGTCACC TTCTGGCACT TCAAATCATT
    AGGGATTTGG TGGACAAAGG TGATGACCTT TTTCTTGATC AATTGGCCCG GCTTGGTGTT
    ATAAGCAAGG TTTCTACACT AGCTGGACCT ACATCAGATG ATGAGAATGA GGAGGATAGC
    AAGCCAGAGA AGGTGAGGAT ACACCTATTT CCTCTGTACA GTGCCCACAA AGGAACACAA
    GCAGCTGCAT CACAACTTAA AGAGGATGAG CCACAGGAAG ATGCCAAGGA GTTGCAACAG
    GGCCGGCCCT ACCATTGGCG GGACTGGTCT GTTATTCGCG GTAGGGACTG TTTGTACATC
    TGGAGTGATG CTGCAGCTCT GGAGCTCTCT AATGGTAGCA ATGGCTGGTT CCGCTTTATC
    CTAGATGGGA AACTGGCAAC CATGTATTCA AGTGGAAGCC CAGAGGGGGG TTCTGATAGC
    TCAGAAAGTA GAAGTGAATT TTTAGAAAAG TTACAGCGGG CCCGCAGCCA GGTCAAACCA
    TCCACTTCTA GCCAGCCTAT CTTGTCCACT CCTGGTCCAG GAAAGCTAAC AGTAGGCAAC
    TGGTCTCTCA CTTGTCTGAA AGATGGTGAG ATTGCAATCC ACAACTCGGA CGGGCAGCAG
    GCTACAATAC TGAAGGAGGA TCTACCAGGC TTTGTGTTTG AGTCAAACAG GGGTACCAAG
    CACTCCTTCA CGGCAGAAAC ATCACTAGGA TCTGAATTTG TAACTGGCTG GACAGGAAAG
    AGAGGCAGAA AGCTGAAGTC TAAGTTGGAG AAAACTAAAC AGAAGGTACG GACAATGGCA
    AGAGATTTGT ATGATGATCA TTTTAAGGCA GTGGAAAGCA TGCCCCGAGG AGTGGTAGTG
    ACGCTTCGAA ATATTGCAAC ACAGCTGGAA TCTGCTTGGG AACTTCATAC AAACAGACAG
    TGTATTGAAG GAGAGAACAC ATGGAGAGAT CTGATGAAAA CTGCCCTGGA AAACCTGATT
    GTGCTACTGA AGGATGAGAA TACTATTTCC CCTTATGAGA TGTGCAGCAG TGGACTGGTG
    CAGGCTTTGC TTACGGTGCT CAATAATAAT GAAGACTGTG ATATAAAACA AGACTGTGGG
    CAGCTGGTGG AGAGACTTAA TGTATTTAAA ACTGCCTTCA GCGAAAATGA AGATGATGAA
    AGCCGCCCTG CCGTTGCCCT CGTCCGGAAG TTGATTGCTG TGCTTGAATC AATAGAACGG
    CTGCCCCTTC ACCTGTATGA CACCCCTGGA TCTAGCTATA ACTTGCAGAT CCTGACCCGT
    AGGCTTCGGT TTCGGCTGGA GCGTGCACCT GGAGAAACGT CTCTAATCGA CCGTACCGGT
    CGGATGCTGA AAATGGAGCC ACTGGCAACT GTAGAGTCCT TGGAGCAGTA TTTACTGAAA
    ATGGTGGCTA AGCAGTGGTA CGACTTTGAC CGTTCGTCCT TTGTGTTTGT GCGCAAATTG
    CGCGAGGGCC AGAGTTGTGT GTTCCGCCAC CAGCATGACT TTGATGATAA TGGAATCATG
    TACTGGATTG GCACCAATGC AAAGACAGCT TATGAGTGGG TCAACCCTGC TGCTTACGGA
    CTAGTAGTTG TTACATCTTC TGAAGGCCGC AATTTGCCAT ATGGCCGCCT TGAGGATATC
    CTGAGCCGTG ATAGCTCTGC CCTTAATTGC CACACTAATG ATGATAAGAG TGCCTGGTTT
    GCCATTGACC TTGGCTTGTG GGTTGTGCCT TCTGCCTATA CACTGCGTCA TGCTCGTGGA
    TATGGCCGTT CCGCTCTTCG CAATTGGGTC TTCCAGGTGT CTAAAGATGG TCAGAACTGG
    ACCACCCTCT ACACCCACAT GGATGACTGC AGCCTGAATG AACCTGGCTC CACAGCCACA
    TGGCCTCTGG ACCCTGCGAG AGAAGAAAAG CAGGGTTGGC GTCATGTCCG GATAAAGCAG
    ACGGGAAAGA ATGCCAGTGG CCAGACCCAC TACCTCTCAC TTTCTGGCTT TGAGCTTTAT
    GGCAATGTGA CTGGGGTGTG TGAAGACCAA CTTGGTAAAG CTGCCAAAGA GGCAGAAGCC
    AACTTAAGGC GCCAGAGGCG GCTGGTACGC TCTCAGGTGC TAAAATATAT GGTGCCTGGA
    GCCCGTGTTA TTCGGGGCAT TGATTGGAAG TGGCGAGACC AAGATGGCAG TGCTCAAGGA
    GAAGGCACTG TTACAGGAGA ACTGCACAAT GGAACACCGC CCTCGTGGAG CAGCCTGGTG
    AAAAACAACT GTCCTGACAA AGCCCCTCCC TCTTCCTCAT CCTCTTGTGT GGTCGTTGGA
    AGCGTGGCGG GCTCAGGCAG CCGCAAAGGA AGCAGCAGCT CGGTCTGTAG CGTGGCAAGC
    AGCAGCGACG TAAGCCTAAG CTGTGCAAAG ACAGAGAGGA GGGCAGAGGA GCAAGTCTCC
    GACATTCACC ATGATCCCAT CCTGCTTCTG TCCTCTAACC AAGCAGCCTC GGGTTCCTCC
    ACCTGCCCCC CTGGAGGAGA GACTGTGGGT GAAGGTGGAG ATAGAAAGGC AGGAGAAGCA
    CCAGCAATCT CAATGGGTAT GGTGAGCATT AGCTCCCCTG ATGTCAGTTC CGTTTCCGAA
    CTCAGCAACA AGGAAGTGGC AGTTCCACGA CCACTGGGAT CTTCTGCCAG CAACAGATTG
    TCTGTCAGTT CACTGTTGGC AGCTGGGGCA CCCATGAGTT CTAGCGCCAG TGTACCAAAC
    TTGTCCTCCA GGGAAACGTC CAGTCTGGAG TCCTTTGTAA GGAGAGTGGC AAATATCGCC
    AGGACAAATG CAACCAACAA CATGAACCTG AGTCGTAGCA GCAGTGATAA CAATACCAAC
    ACTCTGGGGA GGAATGCAGT CAGCTCAGCC ACTTCACCAC TTATGGGAGC TCAGAGCTTT
    CCCAACCTGA CGACAACAGG AACCACATCC ACTGTTACAA TGTCTACATC CAGTGTCACC
    AGCAGTAATG TGGCAACAGC CACTACAGGG TTGTCTGTAG GTCAGTCCCT CAGTAACACC
    CTGACTACAA GCCTAACCTC GACATCCAGT GAGAGTGACA CTGGGCAGGA GGCAGAATAC
    TCCCTGTATG ACTTTCTTGA TAGCTGCCGT GCCAGCACTT TGCTTGCTGA ACTTGATGAT
    GACGAGGATC TCCCTGAGCC CGATGAGGAA GATGATGAAA ACGAAGATGA TAATCAGGAA
    GAGCAAGAGT ATGAAGAAGT TATGGAGGAG GAAGAGTATG AGACCAAAGG TGGCCGACGC
    AGGACCTGGG ATGATGATTA TGTGCTGAAG AGGCAGTTTT CTGCTTTGGT CCCGGCTTTT
    GACCCACGCC CAGGGAGAAC TAATGTTCAG CAGACCACTG ATCTTGAAAT TCCTGCTCCA
    GGCACGCCCC ACTCTGAGCT ACTGGAGGAG GTAGAATGTG CCCCAGCTCC ACGATTAGCA
    CTCACCCTAA AGGTGACAGG CCTTGGGAGC GGGAGAGAGG TGGAGCTCCC CCTGAACAAT
    TTCCGTTCCA CCATATTTTT CTATGTACAG AGACTGCTGC AACTCTCCTG CAATGGAGCC
    ATCAAAACTG ACAAGCTGAG AAGGATTTGG GAGCCCACCT ACACGATTAT GTACAGAGAG
    ATGAAGGATT CAGATAAGCA AAAAGAATGT GGCAGACTGG GCTGTTGGTC TGTAGAGCAT
    GTGGAACAAT CCCTAGGCAC AGATGCCTTG CCAAAGAATG ACCTGATCAC TTACCTGCAG
    CGAAATGCAG ATCCAGGGTT CTTGCGACGC TGGAAACTGA CAGGAACCAA CAAAAGCATT
    AGGAAAAACC GCAACTGTTC CCAGCTTATT GCTGCTTACA AGGACTTCTG TGAGAATGGC
    TGCAAGTCTC TCTCAATGCC AGCTGCTCTT GCCACACTCC AGAGTGCTGA TATCCTTAGT
    CATTCCCGAG AGCAGGCACA AGCCAAAGCG GGCAGCAGCC AAAACTCCTG CGGGGTAGAA
    GATGTTTTGC AGCTCCTGCG AATTCTCTTT ATTGTAGCCA GTGACCCATA TTCTGCCAGG
    ACCCCACAGG AAGATGGAGA AGATATGCTC CTTTTCAGTG TTCCTCCAGA GGAGTTTACT
    AGCAAGAAAA TCACCACAAA GATTGTTCAG CAAATCGAGG AACCTTTAGC TTTAGCAAGT
    GGAGCTTTGC CAGACTGGTG TGAACAGCTG ACCAGCAAAT GTCCCTTCTT AATACCCTTT
    GAAACCCGGC AGCTTTACTT CACATGCACA GCATTTGGTG CCTCAAGGGC TATTGTGTGG
    CTGCAGAACA GGCGGGAAGC CACAGTTGAG AGGAGCAGAA CAGCAAGTGC AGTGCGCAGA
    GACGACCCAG GGGAGTTCAG AGTGGGCCGT CTAAAACACG AACGTGTGAA GGTCCCACGA
    GGGGAGTCTT TGATGGAGTG GGCAGAGAAT GTTATGCAAA TCCACGCAGA CCGAAAATCT
    GTGCTTGAGG TGGAGTTTCT GGGAGAAGAA GGAACAGGCT TGGGCCCTAC CCTTGAGTTT
    TATGCCCTGG TGGCAGCAGA GTTCCAGAAA ACAGATTTGG GGATCTGGTT ATGTGATGAT
    GACTTCCCAG ATGATGAGTC TCGGCAGGTG GATCTAGGTG GTGGTTTGAA GCCCCCTGGT
    TACTATGTGC AGCGTTCCTG TGGCCTCTTC ATTGCACCCT ACCCACAGGA TAGTGAGGAA
    CTAGACCGAG TGACCAGACT TTGCCATTTC CTTGGTGTTT TCCTTGCAAA GTGCATCCAG
    GACAATAGAT TAGTGGACTT GCCCATTTCA AAGCCGTTTT TTAAGCTTAT GTGTATGGGT
    GACATTAAGA GCAACATGAG CAAGCTTTTG TATGCCTCTC GGGGAGAGGA GAGTGAGCAC
    TGCACGGAGA GCCAGTCGGA GGCCTCTACT GAGGATGGGC ATGATGCCCT TTCAGTAGGA
    AGCTTTGAGG AAGACTGCAA ATCTGAGTTC ATCCTAGACC CACCCAAACC AAAGCCACCA
    GCCTGGTTTC AGGGTATTTT AACTTGGGAG GACTTTGAGC TAATAAACCC ACATCGTGCT
    CGCTTCCTTA GGGACATTAG GGAGTTGGCT GTAAAGAGGA GACAGATTCT GGGGAACCGT
    TGCTTATCTG AGGATGAGAA GAACACTCAA CTTCAGGAAC TTATGCTAAA GAATCCTTCT
    GGATCTGGGC CTCCAGTCAG CATTGAGGAT TTGGGACTCA ATTTCCAGTT CTGTCCATCT
    TCCCGGGTTT ATGGCTTCTC TGCAGTCGAT CTAAGGCCAA ATGGAGAGGA TGAGATGGTG
    ACGATAGACA ACGCTGAGGA GTATGTTGAT CTTATGTTTG ATTTCTGCAT GCAGACTGGT
    GTACAAAAGC AAATGGAAGC ATTTAGAAGT GGCTTTAATA AAGTGTTCCC TATGGAGAAA
    CTGGGCTCCT TCAGCCCCGA GGAAGTCCAA ATGATACTGT GTGGAAACCA GTCTCCCTCT
    TGGTCCGCTG AGGACATCAT CAATTACACA GAACCAAAGC TAGGATATAC CCGAGAGAGT
    CCTGGGTTCC TTCGATTCGT GAGAGTCCTA TGTGGCATGT CGTCAGATGA GAGGAAAGCG
    TTCCTGCAGT TTACAACGGG GTGCTCCACA TTGCCTCCAG GAGGACTGGC CAATCTGCAT
    CCGCGTCTCA CAGTCGTGCG CAAGGTTGAT GCCACGGATG CCAGCTACCC ATCTGTAAAT
    ACATGTGTGC ATTACCTGAA GCTGCCGGAG TATTCCTCCG AGGAAATCAT GAGAGACCGC
    CTGCTTGCTG CTACCATGGA GAAAGGATTC CATCTCAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32491
    Clone ID Related Accession (Same CDS sequence) XM_012968096.2 , XM_012968095.2
    Accession Version XM_012968096.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968096.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGGCTGACG TAGATCCAGA CACACTACTG GAATGGCTGC AGATGGGGCA GGGGGACGAG 
    AGAGACATGC AGCTCATTGC CTTGGAACAG CTCTGCATGC TGCTGCTCAT GTCGGATAAT
    GTGGATAGGT GTTTTGAAAC ATGCCCACCT CGCACCTTCC TGCCAGCCTT GTGTAAAATA
    TTCCTGGATG AAAGCGCCCC AGACAATGTG TTGGAAGTAA CAGCCCGTGC AATAACCTAT
    TACCTAGATG TGTCCGCCGA GTGCACGAGG CGTATTGTGG GAGTGGAAGG GGCTATCAAA
    GCTTTGTGCA ATAGGCTTGT GGTGGTGGAG CTCAACAACA GAACCAGTCG GGATTTGGCA
    GAACAGTGTG TCAAGGTGCT GGAGCTTATC TGTACACGTG AGTCTGGGGC TGTCTTTGAG
    GCAGGGGGCT TGAACTGCGT GTTGACATTC ATTCGGGACA GCGGACACCT AGTTCACAAG
    GATACGCTGC ATTCTGCAAT GGCTGTGGTG TCCCGACTGT GTGGTAAGAT GGAGCCACAG
    GATGCATCAT TAGAAACGTG TGTGGAGTCT CTCTCCAGCC TGCTGAAGCA CGAAGACCAT
    CAGGTATCAG ATGGAGCTCT CCGCTGCTTT GCTTCCCTTG CTGACCGCTT TACACGCCGT
    GGTGTAGATC CAGCTCCTCT TGCACAGCAT GGGCTCACTG AGGAACTGCT GTCCCGAATG
    GCAGCAGCCG GAGGCACTGT ATCAGGTCCA TCTTCAGCCT GTAAAACAGG CCGTGGCACC
    AGTGGGGGAC CTTCCACCTC TGGAGACTCA AAAATCAGCA ATCAGGTCTC CACTATAGTC
    AGCCTGTTAT CAACACTATG CCGTGGTTCT CCAGTCGTCA CACATGATTT ACTGCGGGCA
    GAACTCTTAG ACTCCATGGA AAGTGCTCTT CAAGGTGACG AGCGTTGTGT GCTGGATACC
    ATGCGCCTTG TAGACCTTCT TTTGGTGCTG CTTTTCGAAG GCCGCAAAGC TCTGCCTAAG
    TCTAGTGCTG GGTCCACAGG TCGTATTCCT GGGCTGCGGC GGCTTGACAG CTCAGGAGAG
    CGATCTCACC GCCAACTGAT AGACTGCATC CGCAGCAAGG ACACAGATGC TCTCATAGAT
    GCCATTGATA CAGGTGCTTT TGAAGTAAAT TTTATGGATG ATGTTGGCCA GACTCTTCTG
    AATTGGGCCT CTGCTTTTGG CACACAGGAA ATGGTTGAAT TCCTGTGTGA GCGAGGTGCC
    GATGTAAATA GAGGGCAGAG GTCATCTTCA CTGCATTATG CTGCTTGCTT CGGAAGGCCT
    CAGGTGGCAA AGACGTTGCT TCGCCATGGA GCCAACCCAG ATTTAAGAGA TGAGGATGGA
    AAAACACCTT TGGATAAAGC CCGGGAGAGA GGACACAGCG AAGTGGTGGC CATTTTGCAG
    TCCCCAGGGG ACTGGATGTG CCCCGTAAAC AAAGGAGATG AAAAGAAAAA GAAAGACTCT
    AACCGGGAAG AGGAAGAATG CAATGAGCCA AAGGGAGACC CAGAAATGGC CCCCATCTAC
    CTAAAGAGGC TGCTGCCTGT CTTTGCACAG ACCTTTCAGC AAACCATGCT TCCCTCTATC
    AGGAAAGCAA GCTTAGCACT TATTCGGAAG ATGATCCATT TCTGCTCAGA AGCACTGCTT
    AAGGAAGTTT GTCACTCAGA CGCTGGACAC AATTTGCCCA CTGTTCTTGT TGAGATAACA
    GCCACTGTTT TAGACCAGGA GGACGATGAT GATGGTCACC TTCTGGCACT TCAAATCATT
    AGGGATTTGG TGGACAAAGG TGATGACCTT TTTCTTGATC AATTGGCCCG GCTTGGTGTT
    ATAAGCAAGG TTTCTACACT AGCTGGACCT ACATCAGATG ATGAGAATGA GGAGGATAGC
    AAGCCAGAGA AGGTGAGGAT ACACCTATTT CCTCTGTACA GTGCCCACAA AGGAACACAA
    GCAGCTGCAT CACAACTTAA AGAGGATGAG CCACAGGAAG ATGCCAAGGA GTTGCAACAG
    GGCCGGCCCT ACCATTGGCG GGACTGGTCT GTTATTCGCG GTAGGGACTG TTTGTACATC
    TGGAGTGATG CTGCAGCTCT GGAGCTCTCT AATGGTAGCA ATGGCTGGTT CCGCTTTATC
    CTAGATGGGA AACTGGCAAC CATGTATTCA AGTGGAAGCC CAGAGGGGGG TTCTGATAGC
    TCAGAAAGTA GAAGTGAATT TTTAGAAAAG TTACAGCGGG CCCGCAGCCA GGTCAAACCA
    TCCACTTCTA GCCAGCCTAT CTTGTCCACT CCTGGTCCAG GAAAGCTAAC AGTAGGCAAC
    TGGTCTCTCA CTTGTCTGAA AGATGGTGAG ATTGCAATCC ACAACTCGGA CGGGCAGCAG
    GCTACAATAC TGAAGGAGGA TCTACCAGGC TTTGTGTTTG AGTCAAACAG GGGTACCAAG
    CACTCCTTCA CGGCAGAAAC ATCACTAGGA TCTGAATTTG TAACTGGCTG GACAGGAAAG
    AGAGGCAGAA AGCTGAAGTC TAAGTTGGAG AAAACTAAAC AGAAGGTACG GACAATGGCA
    AGAGATTTGT ATGATGATCA TTTTAAGGCA GTGGAAAGCA TGCCCCGAGG AGTGGTAGTG
    ACGCTCCGAA ATATTGCAAC ACAGCTGGAA TCTGCTTGGG AACTTCATAC AAACAGACAG
    TGTATTGAAG GAGAGAACAC ATGGAGAGAT CTGATGAAAA CTGCCCTGGA AAACCTGATT
    GTGCTACTGA AGGATGAGAA TACTATTTCC CCTTATGAGA TGTGCAGCAG TGGACTGGTG
    CAGGCTTTGC TTACGGTGCT CAATAATAAT GAAGACTGTG ATATAAAACA AGACTGTGGG
    CAGCTGGTGG AGAGACTTAA TGTATTTAAA ACTGCCTTCA GCGAAAATGA AGATGATGAA
    AGCCGCCCTG CCGTTGCCCT CGTCCGGAAG TTGATTGCTG TGCTTGAATC AATAGAACGG
    CTGCCCCTTC ACCTGTATGA CACCCCTGGA TCTAGCTATA ACTTGCAGAT CCTGACCCGT
    AGGCTTCGGT TTCGGCTGGA GCGTGCACCT GGAGAAACGT CTCTAATCGA CCGTACCGGT
    CGGATGCTGA AAATGGAGCC ACTGGCAACT GTAGAGTCCT TGGAGCAGTA TTTACTGAAA
    ATGGTGGCTA AGCAGTGGTA CGACTTTGAC CGTTCGTCCT TTGTGTTTGT GCGCAAATTG
    CGCGAGGGCC AGAGTTGTGT GTTCCGCCAC CAGCATGACT TTGATGATAA TGGAATCATG
    TACTGGATTG GCACCAATGC AAAGACAGCT TATGAGTGGG TCAACCCTGC TGCTTACGGA
    CTAGTAGTTG TTACATCTTC TGAAGGCCGC AATTTGCCAT ATGGCCGCCT TGAGGATATC
    CTGAGCCGTG ATAGCTCTGC CCTTAATTGC CACACTAATG ATGATAAGAG TGCCTGGTTT
    GCCATTGACC TTGGCTTGTG GGTTGTGCCT TCTGCCTATA CACTGCGTCA TGCTCGTGGA
    TATGGCCGTT CCGCTCTTCG CAATTGGGTC TTCCAGGTGT CTAAAGATGG TCAGAACTGG
    ACCACCCTCT ACACCCACAT GGATGACTGC AGCCTGAATG AACCTGGCTC CACAGCCACA
    TGGCCTCTGG ACCCTGCGAG AGAAGAAAAG CAGGGTTGGC GTCATGTCCG GATAAAGCAG
    ACGGGAAAGA ATGCCAGTGG CCAGACCCAC TACCTCTCAC TTTCTGGCTT TGAGCTTTAT
    GGCAATGTGA CTGGGGTGTG TGAAGACCAA CTTGGTAAAG CTGCCAAAGA GGCAGAAGCC
    AACTTAAGGC GCCAGAGGCG GCTGGTACGC TCTCAGGTGC TAAAATATAT GGTGCCTGGA
    GCCCGTGTTA TTCGGGGCAT TGATTGGAAG TGGCGAGACC AAGATGGCAG TGCTCAAGGA
    GAAGGCACTG TTACAGGAGA ACTGCACAAT GGCTGGATTG ATGTCATATG GGATGCCGGT
    GGCTCTAACT CTTACCGCAT GGGCGCGGAA GGAAAATTTG ACCTGAAGCT GGCCCCGGGT
    TATGACCCAG AGTCTGCGGT GTCACCCAAA CCTGTTTCAT CCACTGTTGC AGGAACACCG
    CCCTCGTGGA GCAGCCTGGT GAAAAACAAC TGTCCTGACA AAGCCCCTCC CTCTTCCTCA
    TCCTCTTGTG TGGTCGTTGG AAGCGTGGCG GGCTCAGGCA GCCGCAAAGG AAGCAGCAGC
    TCGGTCTGTA GCGTGGCAAG CAGCAGCGAC GTAAGCCTAA GCTGTGCAAA GACAGAGAGG
    AGGGCAGAGG AGCAAGTCTC CGACATTCAC CATGATCCCA TCCTGCTTCT GTCCTCTAAC
    CAAGCAGCCT CGGGTTCCTC CACCTGCCCC CCTGGAGGAG AGACTGTGGG TGAAGGTGGA
    GATAGAAAGG CAGGAGAAGC ACCAGCAATC TCAATGGGTA TGGTGAGCAT TAGCTCCCCT
    GATGTCAGTT CCGTTTCCGA ACTCAGCAAC AAGGAAGTGG CAGTTCCACG ACCACTGGGA
    TCTTCTGCCA GCAACAGATT GTCTGTCAGT TCACTGTTGG CAGCTGGGGC ACCCATGAGT
    TCTAGCGCCA GTGTACCAAA CTTGTCCTCC AGGGAAACGT CCAGTCTGGA GTCCTTTGTA
    AGGAGAGTGG CAAATATCGC CAGGACAAAT GCAACCAACA ACATGAACCT GAGTCGTAGC
    AGCAGTGATA ACAATACCAA CACTCTGGGG AGGAATGCAG TCAGCTCAGC CACTTCACCA
    CTTATGGGAG CTCAGAGCTT TCCCAACCTG ACGACAACAG GAACCACATC CACTGTTACA
    ATGTCTACAT CCAGTGTCAC CAGCAGTAAT GTGGCAACAG CCACTACAGG GTTGTCTGTA
    GGTCAGTCCC TCAGTAACAC CCTGACTACA AGCCTAACCT CGACATCCAG TGAGAGTGAC
    ACTGGGCAGG AGGCAGAATA CTCCCTGTAT GACTTTCTTG ATAGCTGCCG TGCCAGCACT
    TTGCTTGCTG AACTTGATGA TGACGAGGAT CTCCCTGAGC CCGATGAGGA AGATGATGAA
    AACGAAGATG ATAATCAGGA AGAGCAAGAG TATGAAGAAG TTATGGAGGA GGAAGAGTAT
    GAGACCAAAG GTGGCCGACG CAGGACCTGG GATGATGATT ATGTGCTGAA GAGGCAGTTT
    TCTGCTTTGG TCCCGGCTTT TGACCCACGC CCAGGGAGAA CTAATGTTCA GCAGACCACT
    GATCTTGAAA TTCCTGCTCC AGGCACGCCC CACTCTGAGC TACTGGAGGA GGTAGAATGT
    GCCCCAGCTC CACGATTAGC ACTCACCCTA AAGGTGACAG GCCTTGGGAG TGGGAGAGAG
    GTGGAGCTCC CCCTGAACAA TTTCCGTTCC ACCATATTTT TCTATGTACA GAGACTGCTG
    CAACTCTCCT GCAATGGAGC CATCAAAACT GACAAGCTGA GAAGGATTTG GGAGCCCACC
    TACACGATTA TGTACAGAGA GATGAAGGAT TCAGATAAGC AAAAAGAATG TGGCAGACTG
    GGCTGTTGGT CTGTAGAGCA TGTGGAACAA TCCCTAGGCA CAGATGCCTT GCCAAAGAAT
    GACCTGATCA CTTACCTGCA GCGAAATGCA GATCCAGGGT TCTTGCGACG CTGGAAACTG
    ACAGGAACCA ACAAAAGCAT TAGGAAAAAC CGCAACTGTT CCCAGCTTAT TGCTGCTTAC
    AAGGACTTCT GTGAGAATGG CTGCAAGTCT CTCTCAATGC CAGCTGCTCT TGCCACACTC
    CAGAGTGCTG ATATCCTTAG TCATTCCCGA GAGCAGGCAC AAGCCAAAGC GGGCAGCAGC
    CAAAACTCCT GCGGGGTAGA AGATGTTTTG CAGCTCCTGC GAATTCTCTT TATTGTAGCC
    AGTGACCCAT ATTCTGCCAG GACCCCACAG GAAGATGGAG AAGATATGCT CCTTTTCAGT
    GTTCCTCCAG AGGAGTTTAC TAGCAAGAAA ATCACCACAA AGATTGTTCA GCAAATCGAG
    GAACCTTTAG CTTTAGCAAG TGGAGCTTTG CCAGACTGGT GTGAACAGCT GACCAGCAAA
    TGTCCCTTCT TAATACCCTT TGAAACCCGG CAGCTTTACT TCACATGCAC AGCATTTGGT
    GCCTCAAGGG CTATTGTGTG GCTGCAGAAC AGGCGGGAAG CCACAGTTGA GAGGAGCAGA
    ACAGCAAGTG CAGTGCGCAG AGACGACCCA GGGGAGTTCA GAGTGGGCCG TCTAAAACAC
    GAACGTGTGA AGGTCCCACG AGGGGAGTCT TTGATGGAGT GGGCAGAGAA TGTTATGCAA
    ATCCACGCAG ACCGAAAATC TGTGCTTGAG GTGGAGTTTC TGGGAGAAGA AGGAACAGGC
    TTGGGCCCTA CCCTTGAGTT TTATGCCCTG GTGGCAGCAG AGTTCCAGAA AACAGATTTG
    GGGATCTGGT TATGTGATGA TGACTTCCCA GATGATGAGT CTCGGCAGGT GGATCTAGGT
    GGTGGTTTGA AGCCCCCTGG TTACTATGTG CAGCGTTCCT GTGGCCTCTT CATTGCACCC
    TACCCACAGG ATAGTGAGGA ACTAGACCGA GTGACCAGAC TTTGCCATTT CCTTGGTGTT
    TTCCTTGCAA AGTGCATCCA GGACAATAGA TTAGTGGACT TGCCCATTTC AAAGCCGTTT
    TTTAAGCTTA TGTGTATGGG TGACATTAAG AGCAACATGA GCAAGCTTTT GTATGCCTCT
    CGGGGAGAGG AGAGTGAGCA CTGCACGGAG AGCCAGTCGG AGGCCTCTAC TGAGGATGGG
    CATGATGCCC TTTCAGTAGG AAGCTTTGAG GAAGACTGCA AATCTGAGTT CATCCTAGAC
    CCACCCAAAC CAAAGCCACC AGCCTGGTTT CAGGGTATTT TAACTTGGGA GGACTTTGAG
    CTAATAAACC CACATCGTGC TCGCTTCCTT AGGGACATTA GGGAGTTGGC TGTAAAGAGG
    AGACAGATTC TGGGGAACCG TTGCTTATCT GAGGATGAGA AGAACACTCA ACTTCAGGAA
    CTTATGCTAA AGAATCCTTC TGGATCTGGG CCTCCAGTCA GCATTGAGGA TTTGGGACTC
    AATTTCCAGT TCTGTCCATC TTCCCGGGTT TATGGCTTCT CTGCAGTCGA TCTAAGGCCA
    AATGGAGAGG ATGAGATGGT GACGATAGAC AACGCTGAGG AGTATGTTGA TCTTATGTTT
    GATTTCTGCA TGCAGACTGG TGTACAAAAG CAAATGGAAG CATTTAGAAG TGGCTTTAAT
    AAAGTGTTCC CTATGGAGAA ACTGGGCTCC TTCAGCCCCG AGGAAGTCCA AATGATACTG
    TGTGGAAACC AGTCTCCCTC TTGGTCCGCT GAGGACATCA TCAATTACAC AGAACCAAAG
    CTAGGATATA CCCGAGAGAG TCCTGGGTTC CTTCGATTCG TGAGAGTCCT ATGTGGCATG
    TCGTCAGATG AGAGGAAAGC GTTCCTGCAG TTTACAACGG GGTGCTCCAC ATTGCCTCCA
    GGAGGACTGG CCAATCTGCA TCCGCGTCTC ACAGTCGTGC GCAAGGTTGA TGCCACGGAT
    GCCAGCTACC CATCTGTAAA TACATGTGTG CATTACCTGA AGCTGCCGGA GTATTCCTCC
    GAGGAAATCA TGAGAGACCG CCTGCTTGCT GCTACCATGG AGAAAGGATT CCATCTCAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012823550.1
    CDS221..7963
    Translation

    Target ORF information:

    RefSeq Version XM_012968096.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 1 (hectd1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012968096.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGGCTGACG TAGATCCAGA CACACTACTG GAATGGCTGC AGATGGGGCA GGGGGACGAG 
    AGAGACATGC AGCTCATTGC CTTGGAACAG CTCTGCATGC TGCTGCTCAT GTCGGATAAT
    GTGGATAGGT GTTTTGAAAC ATGCCCACCT CGCACCTTCC TGCCAGCCTT GTGTAAAATA
    TTCCTGGATG AAAGCGCCCC AGACAATGTG TTGGAAGTAA CAGCCCGTGC AATAACCTAT
    TACCTAGATG TGTCCGCCGA GTGCACGAGG CGTATTGTGG GAGTGGAAGG GGCTATCAAA
    GCTTTGTGCA ATAGGCTTGT GGTGGTGGAG CTCAACAACA GAACCAGTCG GGATTTGGCA
    GAACAGTGTG TCAAGGTGCT GGAGCTTATC TGTACACGTG AGTCTGGGGC TGTCTTTGAG
    GCAGGGGGCT TGAACTGCGT GTTGACATTC ATTCGGGACA GCGGACACCT AGTTCACAAG
    GATACGCTGC ATTCTGCAAT GGCTGTGGTG TCCCGACTGT GTGGTAAGAT GGAGCCACAG
    GATGCATCAT TAGAAACGTG TGTGGAGTCT CTCTCCAGCC TGCTGAAGCA CGAAGACCAT
    CAGGTATCAG ATGGAGCTCT CCGCTGCTTT GCTTCCCTTG CTGACCGCTT TACACGCCGT
    GGTGTAGATC CAGCTCCTCT TGCACAGCAT GGGCTCACTG AGGAACTGCT GTCCCGAATG
    GCAGCAGCCG GAGGCACTGT ATCAGGTCCA TCTTCAGCCT GTAAAACAGG CCGTGGCACC
    AGTGGGGGAC CTTCCACCTC TGGAGACTCA AAAATCAGCA ATCAGGTCTC CACTATAGTC
    AGCCTGTTAT CAACACTATG CCGTGGTTCT CCAGTCGTCA CACATGATTT ACTGCGGGCA
    GAACTCTTAG ACTCCATGGA AAGTGCTCTT CAAGGTGACG AGCGTTGTGT GCTGGATACC
    ATGCGCCTTG TAGACCTTCT TTTGGTGCTG CTTTTCGAAG GCCGCAAAGC TCTGCCTAAG
    TCTAGTGCTG GGTCCACAGG TCGTATTCCT GGGCTGCGGC GGCTTGACAG CTCAGGAGAG
    CGATCTCACC GCCAACTGAT AGACTGCATC CGCAGCAAGG ACACAGATGC TCTCATAGAT
    GCCATTGATA CAGGTGCTTT TGAAGTAAAT TTTATGGATG ATGTTGGCCA GACTCTTCTG
    AATTGGGCCT CTGCTTTTGG CACACAGGAA ATGGTTGAAT TCCTGTGTGA GCGAGGTGCC
    GATGTAAATA GAGGGCAGAG GTCATCTTCA CTGCATTATG CTGCTTGCTT CGGAAGGCCT
    CAGGTGGCAA AGACGTTGCT TCGCCATGGA GCCAACCCAG ATTTAAGAGA TGAGGATGGA
    AAAACACCTT TGGATAAAGC CCGGGAGAGA GGACACAGCG AAGTGGTGGC CATTTTGCAG
    TCCCCAGGGG ACTGGATGTG CCCCGTAAAC AAAGGAGATG AAAAGAAAAA GAAAGACTCT
    AACCGGGAAG AGGAAGAATG CAATGAGCCA AAGGGAGACC CAGAAATGGC CCCCATCTAC
    CTAAAGAGGC TGCTGCCTGT CTTTGCACAG ACCTTTCAGC AAACCATGCT TCCCTCTATC
    AGGAAAGCAA GCTTAGCACT TATTCGGAAG ATGATCCATT TCTGCTCAGA AGCACTGCTT
    AAGGAAGTTT GTCACTCAGA CGCTGGACAC AATTTGCCCA CTGTTCTTGT TGAGATAACA
    GCCACTGTTT TAGACCAGGA GGACGATGAT GATGGTCACC TTCTGGCACT TCAAATCATT
    AGGGATTTGG TGGACAAAGG TGATGACCTT TTTCTTGATC AATTGGCCCG GCTTGGTGTT
    ATAAGCAAGG TTTCTACACT AGCTGGACCT ACATCAGATG ATGAGAATGA GGAGGATAGC
    AAGCCAGAGA AGGTGAGGAT ACACCTATTT CCTCTGTACA GTGCCCACAA AGGAACACAA
    GCAGCTGCAT CACAACTTAA AGAGGATGAG CCACAGGAAG ATGCCAAGGA GTTGCAACAG
    GGCCGGCCCT ACCATTGGCG GGACTGGTCT GTTATTCGCG GTAGGGACTG TTTGTACATC
    TGGAGTGATG CTGCAGCTCT GGAGCTCTCT AATGGTAGCA ATGGCTGGTT CCGCTTTATC
    CTAGATGGGA AACTGGCAAC CATGTATTCA AGTGGAAGCC CAGAGGGGGG TTCTGATAGC
    TCAGAAAGTA GAAGTGAATT TTTAGAAAAG TTACAGCGGG CCCGCAGCCA GGTCAAACCA
    TCCACTTCTA GCCAGCCTAT CTTGTCCACT CCTGGTCCAG GAAAGCTAAC AGTAGGCAAC
    TGGTCTCTCA CTTGTCTGAA AGATGGTGAG ATTGCAATCC ACAACTCGGA CGGGCAGCAG
    GCTACAATAC TGAAGGAGGA TCTACCAGGC TTTGTGTTTG AGTCAAACAG GGGTACCAAG
    CACTCCTTCA CGGCAGAAAC ATCACTAGGA TCTGAATTTG TAACTGGCTG GACAGGAAAG
    AGAGGCAGAA AGCTGAAGTC TAAGTTGGAG AAAACTAAAC AGAAGGTACG GACAATGGCA
    AGAGATTTGT ATGATGATCA TTTTAAGGCA GTGGAAAGCA TGCCCCGAGG AGTGGTAGTG
    ACGCTCCGAA ATATTGCAAC ACAGCTGGAA TCTGCTTGGG AACTTCATAC AAACAGACAG
    TGTATTGAAG GAGAGAACAC ATGGAGAGAT CTGATGAAAA CTGCCCTGGA AAACCTGATT
    GTGCTACTGA AGGATGAGAA TACTATTTCC CCTTATGAGA TGTGCAGCAG TGGACTGGTG
    CAGGCTTTGC TTACGGTGCT CAATAATAAT GAAGACTGTG ATATAAAACA AGACTGTGGG
    CAGCTGGTGG AGAGACTTAA TGTATTTAAA ACTGCCTTCA GCGAAAATGA AGATGATGAA
    AGCCGCCCTG CCGTTGCCCT CGTCCGGAAG TTGATTGCTG TGCTTGAATC AATAGAACGG
    CTGCCCCTTC ACCTGTATGA CACCCCTGGA TCTAGCTATA ACTTGCAGAT CCTGACCCGT
    AGGCTTCGGT TTCGGCTGGA GCGTGCACCT GGAGAAACGT CTCTAATCGA CCGTACCGGT
    CGGATGCTGA AAATGGAGCC ACTGGCAACT GTAGAGTCCT TGGAGCAGTA TTTACTGAAA
    ATGGTGGCTA AGCAGTGGTA CGACTTTGAC CGTTCGTCCT TTGTGTTTGT GCGCAAATTG
    CGCGAGGGCC AGAGTTGTGT GTTCCGCCAC CAGCATGACT TTGATGATAA TGGAATCATG
    TACTGGATTG GCACCAATGC AAAGACAGCT TATGAGTGGG TCAACCCTGC TGCTTACGGA
    CTAGTAGTTG TTACATCTTC TGAAGGCCGC AATTTGCCAT ATGGCCGCCT TGAGGATATC
    CTGAGCCGTG ATAGCTCTGC CCTTAATTGC CACACTAATG ATGATAAGAG TGCCTGGTTT
    GCCATTGACC TTGGCTTGTG GGTTGTGCCT TCTGCCTATA CACTGCGTCA TGCTCGTGGA
    TATGGCCGTT CCGCTCTTCG CAATTGGGTC TTCCAGGTGT CTAAAGATGG TCAGAACTGG
    ACCACCCTCT ACACCCACAT GGATGACTGC AGCCTGAATG AACCTGGCTC CACAGCCACA
    TGGCCTCTGG ACCCTGCGAG AGAAGAAAAG CAGGGTTGGC GTCATGTCCG GATAAAGCAG
    ACGGGAAAGA ATGCCAGTGG CCAGACCCAC TACCTCTCAC TTTCTGGCTT TGAGCTTTAT
    GGCAATGTGA CTGGGGTGTG TGAAGACCAA CTTGGTAAAG CTGCCAAAGA GGCAGAAGCC
    AACTTAAGGC GCCAGAGGCG GCTGGTACGC TCTCAGGTGC TAAAATATAT GGTGCCTGGA
    GCCCGTGTTA TTCGGGGCAT TGATTGGAAG TGGCGAGACC AAGATGGCAG TGCTCAAGGA
    GAAGGCACTG TTACAGGAGA ACTGCACAAT GGCTGGATTG ATGTCATATG GGATGCCGGT
    GGCTCTAACT CTTACCGCAT GGGCGCGGAA GGAAAATTTG ACCTGAAGCT GGCCCCGGGT
    TATGACCCAG AGTCTGCGGT GTCACCCAAA CCTGTTTCAT CCACTGTTGC AGGAACACCG
    CCCTCGTGGA GCAGCCTGGT GAAAAACAAC TGTCCTGACA AAGCCCCTCC CTCTTCCTCA
    TCCTCTTGTG TGGTCGTTGG AAGCGTGGCG GGCTCAGGCA GCCGCAAAGG AAGCAGCAGC
    TCGGTCTGTA GCGTGGCAAG CAGCAGCGAC GTAAGCCTAA GCTGTGCAAA GACAGAGAGG
    AGGGCAGAGG AGCAAGTCTC CGACATTCAC CATGATCCCA TCCTGCTTCT GTCCTCTAAC
    CAAGCAGCCT CGGGTTCCTC CACCTGCCCC CCTGGAGGAG AGACTGTGGG TGAAGGTGGA
    GATAGAAAGG CAGGAGAAGC ACCAGCAATC TCAATGGGTA TGGTGAGCAT TAGCTCCCCT
    GATGTCAGTT CCGTTTCCGA ACTCAGCAAC AAGGAAGTGG CAGTTCCACG ACCACTGGGA
    TCTTCTGCCA GCAACAGATT GTCTGTCAGT TCACTGTTGG CAGCTGGGGC ACCCATGAGT
    TCTAGCGCCA GTGTACCAAA CTTGTCCTCC AGGGAAACGT CCAGTCTGGA GTCCTTTGTA
    AGGAGAGTGG CAAATATCGC CAGGACAAAT GCAACCAACA ACATGAACCT GAGTCGTAGC
    AGCAGTGATA ACAATACCAA CACTCTGGGG AGGAATGCAG TCAGCTCAGC CACTTCACCA
    CTTATGGGAG CTCAGAGCTT TCCCAACCTG ACGACAACAG GAACCACATC CACTGTTACA
    ATGTCTACAT CCAGTGTCAC CAGCAGTAAT GTGGCAACAG CCACTACAGG GTTGTCTGTA
    GGTCAGTCCC TCAGTAACAC CCTGACTACA AGCCTAACCT CGACATCCAG TGAGAGTGAC
    ACTGGGCAGG AGGCAGAATA CTCCCTGTAT GACTTTCTTG ATAGCTGCCG TGCCAGCACT
    TTGCTTGCTG AACTTGATGA TGACGAGGAT CTCCCTGAGC CCGATGAGGA AGATGATGAA
    AACGAAGATG ATAATCAGGA AGAGCAAGAG TATGAAGAAG TTATGGAGGA GGAAGAGTAT
    GAGACCAAAG GTGGCCGACG CAGGACCTGG GATGATGATT ATGTGCTGAA GAGGCAGTTT
    TCTGCTTTGG TCCCGGCTTT TGACCCACGC CCAGGGAGAA CTAATGTTCA GCAGACCACT
    GATCTTGAAA TTCCTGCTCC AGGCACGCCC CACTCTGAGC TACTGGAGGA GGTAGAATGT
    GCCCCAGCTC CACGATTAGC ACTCACCCTA AAGGTGACAG GCCTTGGGAG TGGGAGAGAG
    GTGGAGCTCC CCCTGAACAA TTTCCGTTCC ACCATATTTT TCTATGTACA GAGACTGCTG
    CAACTCTCCT GCAATGGAGC CATCAAAACT GACAAGCTGA GAAGGATTTG GGAGCCCACC
    TACACGATTA TGTACAGAGA GATGAAGGAT TCAGATAAGC AAAAAGAATG TGGCAGACTG
    GGCTGTTGGT CTGTAGAGCA TGTGGAACAA TCCCTAGGCA CAGATGCCTT GCCAAAGAAT
    GACCTGATCA CTTACCTGCA GCGAAATGCA GATCCAGGGT TCTTGCGACG CTGGAAACTG
    ACAGGAACCA ACAAAAGCAT TAGGAAAAAC CGCAACTGTT CCCAGCTTAT TGCTGCTTAC
    AAGGACTTCT GTGAGAATGG CTGCAAGTCT CTCTCAATGC CAGCTGCTCT TGCCACACTC
    CAGAGTGCTG ATATCCTTAG TCATTCCCGA GAGCAGGCAC AAGCCAAAGC GGGCAGCAGC
    CAAAACTCCT GCGGGGTAGA AGATGTTTTG CAGCTCCTGC GAATTCTCTT TATTGTAGCC
    AGTGACCCAT ATTCTGCCAG GACCCCACAG GAAGATGGAG AAGATATGCT CCTTTTCAGT
    GTTCCTCCAG AGGAGTTTAC TAGCAAGAAA ATCACCACAA AGATTGTTCA GCAAATCGAG
    GAACCTTTAG CTTTAGCAAG TGGAGCTTTG CCAGACTGGT GTGAACAGCT GACCAGCAAA
    TGTCCCTTCT TAATACCCTT TGAAACCCGG CAGCTTTACT TCACATGCAC AGCATTTGGT
    GCCTCAAGGG CTATTGTGTG GCTGCAGAAC AGGCGGGAAG CCACAGTTGA GAGGAGCAGA
    ACAGCAAGTG CAGTGCGCAG AGACGACCCA GGGGAGTTCA GAGTGGGCCG TCTAAAACAC
    GAACGTGTGA AGGTCCCACG AGGGGAGTCT TTGATGGAGT GGGCAGAGAA TGTTATGCAA
    ATCCACGCAG ACCGAAAATC TGTGCTTGAG GTGGAGTTTC TGGGAGAAGA AGGAACAGGC
    TTGGGCCCTA CCCTTGAGTT TTATGCCCTG GTGGCAGCAG AGTTCCAGAA AACAGATTTG
    GGGATCTGGT TATGTGATGA TGACTTCCCA GATGATGAGT CTCGGCAGGT GGATCTAGGT
    GGTGGTTTGA AGCCCCCTGG TTACTATGTG CAGCGTTCCT GTGGCCTCTT CATTGCACCC
    TACCCACAGG ATAGTGAGGA ACTAGACCGA GTGACCAGAC TTTGCCATTT CCTTGGTGTT
    TTCCTTGCAA AGTGCATCCA GGACAATAGA TTAGTGGACT TGCCCATTTC AAAGCCGTTT
    TTTAAGCTTA TGTGTATGGG TGACATTAAG AGCAACATGA GCAAGCTTTT GTATGCCTCT
    CGGGGAGAGG AGAGTGAGCA CTGCACGGAG AGCCAGTCGG AGGCCTCTAC TGAGGATGGG
    CATGATGCCC TTTCAGTAGG AAGCTTTGAG GAAGACTGCA AATCTGAGTT CATCCTAGAC
    CCACCCAAAC CAAAGCCACC AGCCTGGTTT CAGGGTATTT TAACTTGGGA GGACTTTGAG
    CTAATAAACC CACATCGTGC TCGCTTCCTT AGGGACATTA GGGAGTTGGC TGTAAAGAGG
    AGACAGATTC TGGGGAACCG TTGCTTATCT GAGGATGAGA AGAACACTCA ACTTCAGGAA
    CTTATGCTAA AGAATCCTTC TGGATCTGGG CCTCCAGTCA GCATTGAGGA TTTGGGACTC
    AATTTCCAGT TCTGTCCATC TTCCCGGGTT TATGGCTTCT CTGCAGTCGA TCTAAGGCCA
    AATGGAGAGG ATGAGATGGT GACGATAGAC AACGCTGAGG AGTATGTTGA TCTTATGTTT
    GATTTCTGCA TGCAGACTGG TGTACAAAAG CAAATGGAAG CATTTAGAAG TGGCTTTAAT
    AAAGTGTTCC CTATGGAGAA ACTGGGCTCC TTCAGCCCCG AGGAAGTCCA AATGATACTG
    TGTGGAAACC AGTCTCCCTC TTGGTCCGCT GAGGACATCA TCAATTACAC AGAACCAAAG
    CTAGGATATA CCCGAGAGAG TCCTGGGTTC CTTCGATTCG TGAGAGTCCT ATGTGGCATG
    TCGTCAGATG AGAGGAAAGC GTTCCTGCAG TTTACAACGG GGTGCTCCAC ATTGCCTCCA
    GGAGGACTGG CCAATCTGCA TCCGCGTCTC ACAGTCGTGC GCAAGGTTGA TGCCACGGAT
    GCCAGCTACC CATCTGTAAA TACATGTGTG CATTACCTGA AGCTGCCGGA GTATTCCTCC
    GAGGAAATCA TGAGAGACCG CCTGCTTGCT GCTACCATGG AGAAAGGATT CCATCTCAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32491
    Clone ID Related Accession (Same CDS sequence) XM_012968096.2 , XM_012968095.2
    Accession Version XM_012968095.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968095.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGGCTGACG TAGATCCAGA CACACTACTG GAATGGCTGC AGATGGGGCA GGGGGACGAG 
    AGAGACATGC AGCTCATTGC CTTGGAACAG CTCTGCATGC TGCTGCTCAT GTCGGATAAT
    GTGGATAGGT GTTTTGAAAC ATGCCCACCT CGCACCTTCC TGCCAGCCTT GTGTAAAATA
    TTCCTGGATG AAAGCGCCCC AGACAATGTG TTGGAAGTAA CAGCCCGTGC AATAACCTAT
    TACCTAGATG TGTCCGCCGA GTGCACGAGG CGTATTGTGG GAGTGGAAGG GGCTATCAAA
    GCTTTGTGCA ATAGGCTTGT GGTGGTGGAG CTCAACAACA GAACCAGTCG GGATTTGGCA
    GAACAGTGTG TCAAGGTGCT GGAGCTTATC TGTACACGTG AGTCTGGGGC TGTCTTTGAG
    GCAGGGGGCT TGAACTGCGT GTTGACATTC ATTCGGGACA GCGGACACCT AGTTCACAAG
    GATACGCTGC ATTCTGCAAT GGCTGTGGTG TCCCGACTGT GTGGTAAGAT GGAGCCACAG
    GATGCATCAT TAGAAACGTG TGTGGAGTCT CTCTCCAGCC TGCTGAAGCA CGAAGACCAT
    CAGGTATCAG ATGGAGCTCT CCGCTGCTTT GCTTCCCTTG CTGACCGCTT TACACGCCGT
    GGTGTAGATC CAGCTCCTCT TGCACAGCAT GGGCTCACTG AGGAACTGCT GTCCCGAATG
    GCAGCAGCCG GAGGCACTGT ATCAGGTCCA TCTTCAGCCT GTAAAACAGG CCGTGGCACC
    AGTGGGGGAC CTTCCACCTC TGGAGACTCA AAAATCAGCA ATCAGGTCTC CACTATAGTC
    AGCCTGTTAT CAACACTATG CCGTGGTTCT CCAGTCGTCA CACATGATTT ACTGCGGGCA
    GAACTCTTAG ACTCCATGGA AAGTGCTCTT CAAGGTGACG AGCGTTGTGT GCTGGATACC
    ATGCGCCTTG TAGACCTTCT TTTGGTGCTG CTTTTCGAAG GCCGCAAAGC TCTGCCTAAG
    TCTAGTGCTG GGTCCACAGG TCGTATTCCT GGGCTGCGGC GGCTTGACAG CTCAGGAGAG
    CGATCTCACC GCCAACTGAT AGACTGCATC CGCAGCAAGG ACACAGATGC TCTCATAGAT
    GCCATTGATA CAGGTGCTTT TGAAGTAAAT TTTATGGATG ATGTTGGCCA GACTCTTCTG
    AATTGGGCCT CTGCTTTTGG CACACAGGAA ATGGTTGAAT TCCTGTGTGA GCGAGGTGCC
    GATGTAAATA GAGGGCAGAG GTCATCTTCA CTGCATTATG CTGCTTGCTT CGGAAGGCCT
    CAGGTGGCAA AGACGTTGCT TCGCCATGGA GCCAACCCAG ATTTAAGAGA TGAGGATGGA
    AAAACACCTT TGGATAAAGC CCGGGAGAGA GGACACAGCG AAGTGGTGGC CATTTTGCAG
    TCCCCAGGGG ACTGGATGTG CCCCGTAAAC AAAGGAGATG AAAAGAAAAA GAAAGACTCT
    AACCGGGAAG AGGAAGAATG CAATGAGCCA AAGGGAGACC CAGAAATGGC CCCCATCTAC
    CTAAAGAGGC TGCTGCCTGT CTTTGCACAG ACCTTTCAGC AAACCATGCT TCCCTCTATC
    AGGAAAGCAA GCTTAGCACT TATTCGGAAG ATGATCCATT TCTGCTCAGA AGCACTGCTT
    AAGGAAGTTT GTCACTCAGA CGCTGGACAC AATTTGCCCA CTGTTCTTGT TGAGATAACA
    GCCACTGTTT TAGACCAGGA GGACGATGAT GATGGTCACC TTCTGGCACT TCAAATCATT
    AGGGATTTGG TGGACAAAGG TGATGACCTT TTTCTTGATC AATTGGCCCG GCTTGGTGTT
    ATAAGCAAGG TTTCTACACT AGCTGGACCT ACATCAGATG ATGAGAATGA GGAGGATAGC
    AAGCCAGAGA AGGTGAGGAT ACACCTATTT CCTCTGTACA GTGCCCACAA AGGAACACAA
    GCAGCTGCAT CACAACTTAA AGAGGATGAG CCACAGGAAG ATGCCAAGGA GTTGCAACAG
    GGCCGGCCCT ACCATTGGCG GGACTGGTCT GTTATTCGCG GTAGGGACTG TTTGTACATC
    TGGAGTGATG CTGCAGCTCT GGAGCTCTCT AATGGTAGCA ATGGCTGGTT CCGCTTTATC
    CTAGATGGGA AACTGGCAAC CATGTATTCA AGTGGAAGCC CAGAGGGGGG TTCTGATAGC
    TCAGAAAGTA GAAGTGAATT TTTAGAAAAG TTACAGCGGG CCCGCAGCCA GGTCAAACCA
    TCCACTTCTA GCCAGCCTAT CTTGTCCACT CCTGGTCCAG GAAAGCTAAC AGTAGGCAAC
    TGGTCTCTCA CTTGTCTGAA AGATGGTGAG ATTGCAATCC ACAACTCGGA CGGGCAGCAG
    GCTACAATAC TGAAGGAGGA TCTACCAGGC TTTGTGTTTG AGTCAAACAG GGGTACCAAG
    CACTCCTTCA CGGCAGAAAC ATCACTAGGA TCTGAATTTG TAACTGGCTG GACAGGAAAG
    AGAGGCAGAA AGCTGAAGTC TAAGTTGGAG AAAACTAAAC AGAAGGTACG GACAATGGCA
    AGAGATTTGT ATGATGATCA TTTTAAGGCA GTGGAAAGCA TGCCCCGAGG AGTGGTAGTG
    ACGCTCCGAA ATATTGCAAC ACAGCTGGAA TCTGCTTGGG AACTTCATAC AAACAGACAG
    TGTATTGAAG GAGAGAACAC ATGGAGAGAT CTGATGAAAA CTGCCCTGGA AAACCTGATT
    GTGCTACTGA AGGATGAGAA TACTATTTCC CCTTATGAGA TGTGCAGCAG TGGACTGGTG
    CAGGCTTTGC TTACGGTGCT CAATAATAAT GAAGACTGTG ATATAAAACA AGACTGTGGG
    CAGCTGGTGG AGAGACTTAA TGTATTTAAA ACTGCCTTCA GCGAAAATGA AGATGATGAA
    AGCCGCCCTG CCGTTGCCCT CGTCCGGAAG TTGATTGCTG TGCTTGAATC AATAGAACGG
    CTGCCCCTTC ACCTGTATGA CACCCCTGGA TCTAGCTATA ACTTGCAGAT CCTGACCCGT
    AGGCTTCGGT TTCGGCTGGA GCGTGCACCT GGAGAAACGT CTCTAATCGA CCGTACCGGT
    CGGATGCTGA AAATGGAGCC ACTGGCAACT GTAGAGTCCT TGGAGCAGTA TTTACTGAAA
    ATGGTGGCTA AGCAGTGGTA CGACTTTGAC CGTTCGTCCT TTGTGTTTGT GCGCAAATTG
    CGCGAGGGCC AGAGTTGTGT GTTCCGCCAC CAGCATGACT TTGATGATAA TGGAATCATG
    TACTGGATTG GCACCAATGC AAAGACAGCT TATGAGTGGG TCAACCCTGC TGCTTACGGA
    CTAGTAGTTG TTACATCTTC TGAAGGCCGC AATTTGCCAT ATGGCCGCCT TGAGGATATC
    CTGAGCCGTG ATAGCTCTGC CCTTAATTGC CACACTAATG ATGATAAGAG TGCCTGGTTT
    GCCATTGACC TTGGCTTGTG GGTTGTGCCT TCTGCCTATA CACTGCGTCA TGCTCGTGGA
    TATGGCCGTT CCGCTCTTCG CAATTGGGTC TTCCAGGTGT CTAAAGATGG TCAGAACTGG
    ACCACCCTCT ACACCCACAT GGATGACTGC AGCCTGAATG AACCTGGCTC CACAGCCACA
    TGGCCTCTGG ACCCTGCGAG AGAAGAAAAG CAGGGTTGGC GTCATGTCCG GATAAAGCAG
    ACGGGAAAGA ATGCCAGTGG CCAGACCCAC TACCTCTCAC TTTCTGGCTT TGAGCTTTAT
    GGCAATGTGA CTGGGGTGTG TGAAGACCAA CTTGGTAAAG CTGCCAAAGA GGCAGAAGCC
    AACTTAAGGC GCCAGAGGCG GCTGGTACGC TCTCAGGTGC TAAAATATAT GGTGCCTGGA
    GCCCGTGTTA TTCGGGGCAT TGATTGGAAG TGGCGAGACC AAGATGGCAG TGCTCAAGGA
    GAAGGCACTG TTACAGGAGA ACTGCACAAT GGCTGGATTG ATGTCATATG GGATGCCGGT
    GGCTCTAACT CTTACCGCAT GGGCGCGGAA GGAAAATTTG ACCTGAAGCT GGCCCCGGGT
    TATGACCCAG AGTCTGCGGT GTCACCCAAA CCTGTTTCAT CCACTGTTGC AGGAACACCG
    CCCTCGTGGA GCAGCCTGGT GAAAAACAAC TGTCCTGACA AAGCCCCTCC CTCTTCCTCA
    TCCTCTTGTG TGGTCGTTGG AAGCGTGGCG GGCTCAGGCA GCCGCAAAGG AAGCAGCAGC
    TCGGTCTGTA GCGTGGCAAG CAGCAGCGAC GTAAGCCTAA GCTGTGCAAA GACAGAGAGG
    AGGGCAGAGG AGCAAGTCTC CGACATTCAC CATGATCCCA TCCTGCTTCT GTCCTCTAAC
    CAAGCAGCCT CGGGTTCCTC CACCTGCCCC CCTGGAGGAG AGACTGTGGG TGAAGGTGGA
    GATAGAAAGG CAGGAGAAGC ACCAGCAATC TCAATGGGTA TGGTGAGCAT TAGCTCCCCT
    GATGTCAGTT CCGTTTCCGA ACTCAGCAAC AAGGAAGTGG CAGTTCCACG ACCACTGGGA
    TCTTCTGCCA GCAACAGATT GTCTGTCAGT TCACTGTTGG CAGCTGGGGC ACCCATGAGT
    TCTAGCGCCA GTGTACCAAA CTTGTCCTCC AGGGAAACGT CCAGTCTGGA GTCCTTTGTA
    AGGAGAGTGG CAAATATCGC CAGGACAAAT GCAACCAACA ACATGAACCT GAGTCGTAGC
    AGCAGTGATA ACAATACCAA CACTCTGGGG AGGAATGCAG TCAGCTCAGC CACTTCACCA
    CTTATGGGAG CTCAGAGCTT TCCCAACCTG ACGACAACAG GAACCACATC CACTGTTACA
    ATGTCTACAT CCAGTGTCAC CAGCAGTAAT GTGGCAACAG CCACTACAGG GTTGTCTGTA
    GGTCAGTCCC TCAGTAACAC CCTGACTACA AGCCTAACCT CGACATCCAG TGAGAGTGAC
    ACTGGGCAGG AGGCAGAATA CTCCCTGTAT GACTTTCTTG ATAGCTGCCG TGCCAGCACT
    TTGCTTGCTG AACTTGATGA TGACGAGGAT CTCCCTGAGC CCGATGAGGA AGATGATGAA
    AACGAAGATG ATAATCAGGA AGAGCAAGAG TATGAAGAAG TTATGGAGGA GGAAGAGTAT
    GAGACCAAAG GTGGCCGACG CAGGACCTGG GATGATGATT ATGTGCTGAA GAGGCAGTTT
    TCTGCTTTGG TCCCGGCTTT TGACCCACGC CCAGGGAGAA CTAATGTTCA GCAGACCACT
    GATCTTGAAA TTCCTGCTCC AGGCACGCCC CACTCTGAGC TACTGGAGGA GGTAGAATGT
    GCCCCAGCTC CACGATTAGC ACTCACCCTA AAGGTGACAG GCCTTGGGAG TGGGAGAGAG
    GTGGAGCTCC CCCTGAACAA TTTCCGTTCC ACCATATTTT TCTATGTACA GAGACTGCTG
    CAACTCTCCT GCAATGGAGC CATCAAAACT GACAAGCTGA GAAGGATTTG GGAGCCCACC
    TACACGATTA TGTACAGAGA GATGAAGGAT TCAGATAAGC AAAAAGAATG TGGCAGACTG
    GGCTGTTGGT CTGTAGAGCA TGTGGAACAA TCCCTAGGCA CAGATGCCTT GCCAAAGAAT
    GACCTGATCA CTTACCTGCA GCGAAATGCA GATCCAGGGT TCTTGCGACG CTGGAAACTG
    ACAGGAACCA ACAAAAGCAT TAGGAAAAAC CGCAACTGTT CCCAGCTTAT TGCTGCTTAC
    AAGGACTTCT GTGAGAATGG CTGCAAGTCT CTCTCAATGC CAGCTGCTCT TGCCACACTC
    CAGAGTGCTG ATATCCTTAG TCATTCCCGA GAGCAGGCAC AAGCCAAAGC GGGCAGCAGC
    CAAAACTCCT GCGGGGTAGA AGATGTTTTG CAGCTCCTGC GAATTCTCTT TATTGTAGCC
    AGTGACCCAT ATTCTGCCAG GACCCCACAG GAAGATGGAG AAGATATGCT CCTTTTCAGT
    GTTCCTCCAG AGGAGTTTAC TAGCAAGAAA ATCACCACAA AGATTGTTCA GCAAATCGAG
    GAACCTTTAG CTTTAGCAAG TGGAGCTTTG CCAGACTGGT GTGAACAGCT GACCAGCAAA
    TGTCCCTTCT TAATACCCTT TGAAACCCGG CAGCTTTACT TCACATGCAC AGCATTTGGT
    GCCTCAAGGG CTATTGTGTG GCTGCAGAAC AGGCGGGAAG CCACAGTTGA GAGGAGCAGA
    ACAGCAAGTG CAGTGCGCAG AGACGACCCA GGGGAGTTCA GAGTGGGCCG TCTAAAACAC
    GAACGTGTGA AGGTCCCACG AGGGGAGTCT TTGATGGAGT GGGCAGAGAA TGTTATGCAA
    ATCCACGCAG ACCGAAAATC TGTGCTTGAG GTGGAGTTTC TGGGAGAAGA AGGAACAGGC
    TTGGGCCCTA CCCTTGAGTT TTATGCCCTG GTGGCAGCAG AGTTCCAGAA AACAGATTTG
    GGGATCTGGT TATGTGATGA TGACTTCCCA GATGATGAGT CTCGGCAGGT GGATCTAGGT
    GGTGGTTTGA AGCCCCCTGG TTACTATGTG CAGCGTTCCT GTGGCCTCTT CATTGCACCC
    TACCCACAGG ATAGTGAGGA ACTAGACCGA GTGACCAGAC TTTGCCATTT CCTTGGTGTT
    TTCCTTGCAA AGTGCATCCA GGACAATAGA TTAGTGGACT TGCCCATTTC AAAGCCGTTT
    TTTAAGCTTA TGTGTATGGG TGACATTAAG AGCAACATGA GCAAGCTTTT GTATGCCTCT
    CGGGGAGAGG AGAGTGAGCA CTGCACGGAG AGCCAGTCGG AGGCCTCTAC TGAGGATGGG
    CATGATGCCC TTTCAGTAGG AAGCTTTGAG GAAGACTGCA AATCTGAGTT CATCCTAGAC
    CCACCCAAAC CAAAGCCACC AGCCTGGTTT CAGGGTATTT TAACTTGGGA GGACTTTGAG
    CTAATAAACC CACATCGTGC TCGCTTCCTT AGGGACATTA GGGAGTTGGC TGTAAAGAGG
    AGACAGATTC TGGGGAACCG TTGCTTATCT GAGGATGAGA AGAACACTCA ACTTCAGGAA
    CTTATGCTAA AGAATCCTTC TGGATCTGGG CCTCCAGTCA GCATTGAGGA TTTGGGACTC
    AATTTCCAGT TCTGTCCATC TTCCCGGGTT TATGGCTTCT CTGCAGTCGA TCTAAGGCCA
    AATGGAGAGG ATGAGATGGT GACGATAGAC AACGCTGAGG AGTATGTTGA TCTTATGTTT
    GATTTCTGCA TGCAGACTGG TGTACAAAAG CAAATGGAAG CATTTAGAAG TGGCTTTAAT
    AAAGTGTTCC CTATGGAGAA ACTGGGCTCC TTCAGCCCCG AGGAAGTCCA AATGATACTG
    TGTGGAAACC AGTCTCCCTC TTGGTCCGCT GAGGACATCA TCAATTACAC AGAACCAAAG
    CTAGGATATA CCCGAGAGAG TCCTGGGTTC CTTCGATTCG TGAGAGTCCT ATGTGGCATG
    TCGTCAGATG AGAGGAAAGC GTTCCTGCAG TTTACAACGG GGTGCTCCAC ATTGCCTCCA
    GGAGGACTGG CCAATCTGCA TCCGCGTCTC ACAGTCGTGC GCAAGGTTGA TGCCACGGAT
    GCCAGCTACC CATCTGTAAA TACATGTGTG CATTACCTGA AGCTGCCGGA GTATTCCTCC
    GAGGAAATCA TGAGAGACCG CCTGCTTGCT GCTACCATGG AGAAAGGATT CCATCTCAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012823549.1
    CDS316..8058
    Translation

    Target ORF information:

    RefSeq Version XM_012968095.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 1 (hectd1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012968095.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGGCTGACG TAGATCCAGA CACACTACTG GAATGGCTGC AGATGGGGCA GGGGGACGAG 
    AGAGACATGC AGCTCATTGC CTTGGAACAG CTCTGCATGC TGCTGCTCAT GTCGGATAAT
    GTGGATAGGT GTTTTGAAAC ATGCCCACCT CGCACCTTCC TGCCAGCCTT GTGTAAAATA
    TTCCTGGATG AAAGCGCCCC AGACAATGTG TTGGAAGTAA CAGCCCGTGC AATAACCTAT
    TACCTAGATG TGTCCGCCGA GTGCACGAGG CGTATTGTGG GAGTGGAAGG GGCTATCAAA
    GCTTTGTGCA ATAGGCTTGT GGTGGTGGAG CTCAACAACA GAACCAGTCG GGATTTGGCA
    GAACAGTGTG TCAAGGTGCT GGAGCTTATC TGTACACGTG AGTCTGGGGC TGTCTTTGAG
    GCAGGGGGCT TGAACTGCGT GTTGACATTC ATTCGGGACA GCGGACACCT AGTTCACAAG
    GATACGCTGC ATTCTGCAAT GGCTGTGGTG TCCCGACTGT GTGGTAAGAT GGAGCCACAG
    GATGCATCAT TAGAAACGTG TGTGGAGTCT CTCTCCAGCC TGCTGAAGCA CGAAGACCAT
    CAGGTATCAG ATGGAGCTCT CCGCTGCTTT GCTTCCCTTG CTGACCGCTT TACACGCCGT
    GGTGTAGATC CAGCTCCTCT TGCACAGCAT GGGCTCACTG AGGAACTGCT GTCCCGAATG
    GCAGCAGCCG GAGGCACTGT ATCAGGTCCA TCTTCAGCCT GTAAAACAGG CCGTGGCACC
    AGTGGGGGAC CTTCCACCTC TGGAGACTCA AAAATCAGCA ATCAGGTCTC CACTATAGTC
    AGCCTGTTAT CAACACTATG CCGTGGTTCT CCAGTCGTCA CACATGATTT ACTGCGGGCA
    GAACTCTTAG ACTCCATGGA AAGTGCTCTT CAAGGTGACG AGCGTTGTGT GCTGGATACC
    ATGCGCCTTG TAGACCTTCT TTTGGTGCTG CTTTTCGAAG GCCGCAAAGC TCTGCCTAAG
    TCTAGTGCTG GGTCCACAGG TCGTATTCCT GGGCTGCGGC GGCTTGACAG CTCAGGAGAG
    CGATCTCACC GCCAACTGAT AGACTGCATC CGCAGCAAGG ACACAGATGC TCTCATAGAT
    GCCATTGATA CAGGTGCTTT TGAAGTAAAT TTTATGGATG ATGTTGGCCA GACTCTTCTG
    AATTGGGCCT CTGCTTTTGG CACACAGGAA ATGGTTGAAT TCCTGTGTGA GCGAGGTGCC
    GATGTAAATA GAGGGCAGAG GTCATCTTCA CTGCATTATG CTGCTTGCTT CGGAAGGCCT
    CAGGTGGCAA AGACGTTGCT TCGCCATGGA GCCAACCCAG ATTTAAGAGA TGAGGATGGA
    AAAACACCTT TGGATAAAGC CCGGGAGAGA GGACACAGCG AAGTGGTGGC CATTTTGCAG
    TCCCCAGGGG ACTGGATGTG CCCCGTAAAC AAAGGAGATG AAAAGAAAAA GAAAGACTCT
    AACCGGGAAG AGGAAGAATG CAATGAGCCA AAGGGAGACC CAGAAATGGC CCCCATCTAC
    CTAAAGAGGC TGCTGCCTGT CTTTGCACAG ACCTTTCAGC AAACCATGCT TCCCTCTATC
    AGGAAAGCAA GCTTAGCACT TATTCGGAAG ATGATCCATT TCTGCTCAGA AGCACTGCTT
    AAGGAAGTTT GTCACTCAGA CGCTGGACAC AATTTGCCCA CTGTTCTTGT TGAGATAACA
    GCCACTGTTT TAGACCAGGA GGACGATGAT GATGGTCACC TTCTGGCACT TCAAATCATT
    AGGGATTTGG TGGACAAAGG TGATGACCTT TTTCTTGATC AATTGGCCCG GCTTGGTGTT
    ATAAGCAAGG TTTCTACACT AGCTGGACCT ACATCAGATG ATGAGAATGA GGAGGATAGC
    AAGCCAGAGA AGGTGAGGAT ACACCTATTT CCTCTGTACA GTGCCCACAA AGGAACACAA
    GCAGCTGCAT CACAACTTAA AGAGGATGAG CCACAGGAAG ATGCCAAGGA GTTGCAACAG
    GGCCGGCCCT ACCATTGGCG GGACTGGTCT GTTATTCGCG GTAGGGACTG TTTGTACATC
    TGGAGTGATG CTGCAGCTCT GGAGCTCTCT AATGGTAGCA ATGGCTGGTT CCGCTTTATC
    CTAGATGGGA AACTGGCAAC CATGTATTCA AGTGGAAGCC CAGAGGGGGG TTCTGATAGC
    TCAGAAAGTA GAAGTGAATT TTTAGAAAAG TTACAGCGGG CCCGCAGCCA GGTCAAACCA
    TCCACTTCTA GCCAGCCTAT CTTGTCCACT CCTGGTCCAG GAAAGCTAAC AGTAGGCAAC
    TGGTCTCTCA CTTGTCTGAA AGATGGTGAG ATTGCAATCC ACAACTCGGA CGGGCAGCAG
    GCTACAATAC TGAAGGAGGA TCTACCAGGC TTTGTGTTTG AGTCAAACAG GGGTACCAAG
    CACTCCTTCA CGGCAGAAAC ATCACTAGGA TCTGAATTTG TAACTGGCTG GACAGGAAAG
    AGAGGCAGAA AGCTGAAGTC TAAGTTGGAG AAAACTAAAC AGAAGGTACG GACAATGGCA
    AGAGATTTGT ATGATGATCA TTTTAAGGCA GTGGAAAGCA TGCCCCGAGG AGTGGTAGTG
    ACGCTCCGAA ATATTGCAAC ACAGCTGGAA TCTGCTTGGG AACTTCATAC AAACAGACAG
    TGTATTGAAG GAGAGAACAC ATGGAGAGAT CTGATGAAAA CTGCCCTGGA AAACCTGATT
    GTGCTACTGA AGGATGAGAA TACTATTTCC CCTTATGAGA TGTGCAGCAG TGGACTGGTG
    CAGGCTTTGC TTACGGTGCT CAATAATAAT GAAGACTGTG ATATAAAACA AGACTGTGGG
    CAGCTGGTGG AGAGACTTAA TGTATTTAAA ACTGCCTTCA GCGAAAATGA AGATGATGAA
    AGCCGCCCTG CCGTTGCCCT CGTCCGGAAG TTGATTGCTG TGCTTGAATC AATAGAACGG
    CTGCCCCTTC ACCTGTATGA CACCCCTGGA TCTAGCTATA ACTTGCAGAT CCTGACCCGT
    AGGCTTCGGT TTCGGCTGGA GCGTGCACCT GGAGAAACGT CTCTAATCGA CCGTACCGGT
    CGGATGCTGA AAATGGAGCC ACTGGCAACT GTAGAGTCCT TGGAGCAGTA TTTACTGAAA
    ATGGTGGCTA AGCAGTGGTA CGACTTTGAC CGTTCGTCCT TTGTGTTTGT GCGCAAATTG
    CGCGAGGGCC AGAGTTGTGT GTTCCGCCAC CAGCATGACT TTGATGATAA TGGAATCATG
    TACTGGATTG GCACCAATGC AAAGACAGCT TATGAGTGGG TCAACCCTGC TGCTTACGGA
    CTAGTAGTTG TTACATCTTC TGAAGGCCGC AATTTGCCAT ATGGCCGCCT TGAGGATATC
    CTGAGCCGTG ATAGCTCTGC CCTTAATTGC CACACTAATG ATGATAAGAG TGCCTGGTTT
    GCCATTGACC TTGGCTTGTG GGTTGTGCCT TCTGCCTATA CACTGCGTCA TGCTCGTGGA
    TATGGCCGTT CCGCTCTTCG CAATTGGGTC TTCCAGGTGT CTAAAGATGG TCAGAACTGG
    ACCACCCTCT ACACCCACAT GGATGACTGC AGCCTGAATG AACCTGGCTC CACAGCCACA
    TGGCCTCTGG ACCCTGCGAG AGAAGAAAAG CAGGGTTGGC GTCATGTCCG GATAAAGCAG
    ACGGGAAAGA ATGCCAGTGG CCAGACCCAC TACCTCTCAC TTTCTGGCTT TGAGCTTTAT
    GGCAATGTGA CTGGGGTGTG TGAAGACCAA CTTGGTAAAG CTGCCAAAGA GGCAGAAGCC
    AACTTAAGGC GCCAGAGGCG GCTGGTACGC TCTCAGGTGC TAAAATATAT GGTGCCTGGA
    GCCCGTGTTA TTCGGGGCAT TGATTGGAAG TGGCGAGACC AAGATGGCAG TGCTCAAGGA
    GAAGGCACTG TTACAGGAGA ACTGCACAAT GGCTGGATTG ATGTCATATG GGATGCCGGT
    GGCTCTAACT CTTACCGCAT GGGCGCGGAA GGAAAATTTG ACCTGAAGCT GGCCCCGGGT
    TATGACCCAG AGTCTGCGGT GTCACCCAAA CCTGTTTCAT CCACTGTTGC AGGAACACCG
    CCCTCGTGGA GCAGCCTGGT GAAAAACAAC TGTCCTGACA AAGCCCCTCC CTCTTCCTCA
    TCCTCTTGTG TGGTCGTTGG AAGCGTGGCG GGCTCAGGCA GCCGCAAAGG AAGCAGCAGC
    TCGGTCTGTA GCGTGGCAAG CAGCAGCGAC GTAAGCCTAA GCTGTGCAAA GACAGAGAGG
    AGGGCAGAGG AGCAAGTCTC CGACATTCAC CATGATCCCA TCCTGCTTCT GTCCTCTAAC
    CAAGCAGCCT CGGGTTCCTC CACCTGCCCC CCTGGAGGAG AGACTGTGGG TGAAGGTGGA
    GATAGAAAGG CAGGAGAAGC ACCAGCAATC TCAATGGGTA TGGTGAGCAT TAGCTCCCCT
    GATGTCAGTT CCGTTTCCGA ACTCAGCAAC AAGGAAGTGG CAGTTCCACG ACCACTGGGA
    TCTTCTGCCA GCAACAGATT GTCTGTCAGT TCACTGTTGG CAGCTGGGGC ACCCATGAGT
    TCTAGCGCCA GTGTACCAAA CTTGTCCTCC AGGGAAACGT CCAGTCTGGA GTCCTTTGTA
    AGGAGAGTGG CAAATATCGC CAGGACAAAT GCAACCAACA ACATGAACCT GAGTCGTAGC
    AGCAGTGATA ACAATACCAA CACTCTGGGG AGGAATGCAG TCAGCTCAGC CACTTCACCA
    CTTATGGGAG CTCAGAGCTT TCCCAACCTG ACGACAACAG GAACCACATC CACTGTTACA
    ATGTCTACAT CCAGTGTCAC CAGCAGTAAT GTGGCAACAG CCACTACAGG GTTGTCTGTA
    GGTCAGTCCC TCAGTAACAC CCTGACTACA AGCCTAACCT CGACATCCAG TGAGAGTGAC
    ACTGGGCAGG AGGCAGAATA CTCCCTGTAT GACTTTCTTG ATAGCTGCCG TGCCAGCACT
    TTGCTTGCTG AACTTGATGA TGACGAGGAT CTCCCTGAGC CCGATGAGGA AGATGATGAA
    AACGAAGATG ATAATCAGGA AGAGCAAGAG TATGAAGAAG TTATGGAGGA GGAAGAGTAT
    GAGACCAAAG GTGGCCGACG CAGGACCTGG GATGATGATT ATGTGCTGAA GAGGCAGTTT
    TCTGCTTTGG TCCCGGCTTT TGACCCACGC CCAGGGAGAA CTAATGTTCA GCAGACCACT
    GATCTTGAAA TTCCTGCTCC AGGCACGCCC CACTCTGAGC TACTGGAGGA GGTAGAATGT
    GCCCCAGCTC CACGATTAGC ACTCACCCTA AAGGTGACAG GCCTTGGGAG TGGGAGAGAG
    GTGGAGCTCC CCCTGAACAA TTTCCGTTCC ACCATATTTT TCTATGTACA GAGACTGCTG
    CAACTCTCCT GCAATGGAGC CATCAAAACT GACAAGCTGA GAAGGATTTG GGAGCCCACC
    TACACGATTA TGTACAGAGA GATGAAGGAT TCAGATAAGC AAAAAGAATG TGGCAGACTG
    GGCTGTTGGT CTGTAGAGCA TGTGGAACAA TCCCTAGGCA CAGATGCCTT GCCAAAGAAT
    GACCTGATCA CTTACCTGCA GCGAAATGCA GATCCAGGGT TCTTGCGACG CTGGAAACTG
    ACAGGAACCA ACAAAAGCAT TAGGAAAAAC CGCAACTGTT CCCAGCTTAT TGCTGCTTAC
    AAGGACTTCT GTGAGAATGG CTGCAAGTCT CTCTCAATGC CAGCTGCTCT TGCCACACTC
    CAGAGTGCTG ATATCCTTAG TCATTCCCGA GAGCAGGCAC AAGCCAAAGC GGGCAGCAGC
    CAAAACTCCT GCGGGGTAGA AGATGTTTTG CAGCTCCTGC GAATTCTCTT TATTGTAGCC
    AGTGACCCAT ATTCTGCCAG GACCCCACAG GAAGATGGAG AAGATATGCT CCTTTTCAGT
    GTTCCTCCAG AGGAGTTTAC TAGCAAGAAA ATCACCACAA AGATTGTTCA GCAAATCGAG
    GAACCTTTAG CTTTAGCAAG TGGAGCTTTG CCAGACTGGT GTGAACAGCT GACCAGCAAA
    TGTCCCTTCT TAATACCCTT TGAAACCCGG CAGCTTTACT TCACATGCAC AGCATTTGGT
    GCCTCAAGGG CTATTGTGTG GCTGCAGAAC AGGCGGGAAG CCACAGTTGA GAGGAGCAGA
    ACAGCAAGTG CAGTGCGCAG AGACGACCCA GGGGAGTTCA GAGTGGGCCG TCTAAAACAC
    GAACGTGTGA AGGTCCCACG AGGGGAGTCT TTGATGGAGT GGGCAGAGAA TGTTATGCAA
    ATCCACGCAG ACCGAAAATC TGTGCTTGAG GTGGAGTTTC TGGGAGAAGA AGGAACAGGC
    TTGGGCCCTA CCCTTGAGTT TTATGCCCTG GTGGCAGCAG AGTTCCAGAA AACAGATTTG
    GGGATCTGGT TATGTGATGA TGACTTCCCA GATGATGAGT CTCGGCAGGT GGATCTAGGT
    GGTGGTTTGA AGCCCCCTGG TTACTATGTG CAGCGTTCCT GTGGCCTCTT CATTGCACCC
    TACCCACAGG ATAGTGAGGA ACTAGACCGA GTGACCAGAC TTTGCCATTT CCTTGGTGTT
    TTCCTTGCAA AGTGCATCCA GGACAATAGA TTAGTGGACT TGCCCATTTC AAAGCCGTTT
    TTTAAGCTTA TGTGTATGGG TGACATTAAG AGCAACATGA GCAAGCTTTT GTATGCCTCT
    CGGGGAGAGG AGAGTGAGCA CTGCACGGAG AGCCAGTCGG AGGCCTCTAC TGAGGATGGG
    CATGATGCCC TTTCAGTAGG AAGCTTTGAG GAAGACTGCA AATCTGAGTT CATCCTAGAC
    CCACCCAAAC CAAAGCCACC AGCCTGGTTT CAGGGTATTT TAACTTGGGA GGACTTTGAG
    CTAATAAACC CACATCGTGC TCGCTTCCTT AGGGACATTA GGGAGTTGGC TGTAAAGAGG
    AGACAGATTC TGGGGAACCG TTGCTTATCT GAGGATGAGA AGAACACTCA ACTTCAGGAA
    CTTATGCTAA AGAATCCTTC TGGATCTGGG CCTCCAGTCA GCATTGAGGA TTTGGGACTC
    AATTTCCAGT TCTGTCCATC TTCCCGGGTT TATGGCTTCT CTGCAGTCGA TCTAAGGCCA
    AATGGAGAGG ATGAGATGGT GACGATAGAC AACGCTGAGG AGTATGTTGA TCTTATGTTT
    GATTTCTGCA TGCAGACTGG TGTACAAAAG CAAATGGAAG CATTTAGAAG TGGCTTTAAT
    AAAGTGTTCC CTATGGAGAA ACTGGGCTCC TTCAGCCCCG AGGAAGTCCA AATGATACTG
    TGTGGAAACC AGTCTCCCTC TTGGTCCGCT GAGGACATCA TCAATTACAC AGAACCAAAG
    CTAGGATATA CCCGAGAGAG TCCTGGGTTC CTTCGATTCG TGAGAGTCCT ATGTGGCATG
    TCGTCAGATG AGAGGAAAGC GTTCCTGCAG TTTACAACGG GGTGCTCCAC ATTGCCTCCA
    GGAGGACTGG CCAATCTGCA TCCGCGTCTC ACAGTCGTGC GCAAGGTTGA TGCCACGGAT
    GCCAGCTACC CATCTGTAAA TACATGTGTG CATTACCTGA AGCTGCCGGA GTATTCCTCC
    GAGGAAATCA TGAGAGACCG CCTGCTTGCT GCTACCATGG AGAAAGGATT CCATCTCAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32492
    Clone ID Related Accession (Same CDS sequence) XM_012968097.2
    Accession Version XM_012968097.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7674bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968097.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGCTGACG TAGATCCAGA CACACTACTG GAATGGCTGC AGATGGGGCA GGGGGACGAG 
    AGAGACATGC AGCTCATTGC CTTGGAACAG CTCTGCATGC TGCTGCTCAT GTCGGATAAT
    GTGGATAGGT GTTTTGAAAC ATGCCCACCT CGCACCTTCC TGCCAGCCTT GTGTAAAATA
    TTCCTGGATG AAAGCGCCCC AGACAATGTG TTGGAAGTAA CAGCCCGTGC AATAACCTAT
    TACCTAGATG TGTCCGCCGA GTGCACGAGG CGTATTGTGG GAGTGGAAGG GGCTATCAAA
    GCTTTGTGCA ATAGGCTTGT GGTGGTGGAG CTCAACAACA GAACCAGTCG GGATTTGGCA
    GAACAGTGTG TCAAGGTGCT GGAGCTTATC TGTACACGTG AGTCTGGGGC TGTCTTTGAG
    GCAGGGGGCT TGAACTGCGT GTTGACATTC ATTCGGGACA GCGGACACCT AGTTCACAAG
    GATACGCTGC ATTCTGCAAT GGCTGTGGTG TCCCGACTGT GTGGTAAGAT GGAGCCACAG
    GATGCATCAT TAGAAACGTG TGTGGAGTCT CTCTCCAGCC TGCTGAAGCA CGAAGACCAT
    CAGGTATCAG ATGGAGCTCT CCGCTGCTTT GCTTCCCTTG CTGACCGCTT TACACGCCGT
    GGTGTAGATC CAGCTCCTCT TGCACAGCAT GGGCTCACTG AGGAACTGCT GTCCCGAATG
    GCAGCAGCCG GAGGCACTGT ATCAGGTCCA TCTTCAGCCT GTAAAACAGG CCGTGGCACC
    AGTGGGGGAC CTTCCACCTC TGGAGACTCA AAAATCAGCA ATCAGGTCTC CACTATAGTC
    AGCCTGTTAT CAACACTATG CCGTGGTTCT CCAGTCGTCA CACATGATTT ACTGCGGGCA
    GAACTCTTAG ACTCCATGGA AAGTGCTCTT CAAGGTGACG AGCGTTGTGT GCTGGATACC
    ATGCGCCTTG TAGACCTTCT TTTGGTGCTG CTTTTCGAAG GCCGCAAAGC TCTGCCTAAG
    TCTAGTGCTG GGTCCACAGG TCGTATTCCT GGGCTGCGGC GGCTTGACAG CTCAGGAGAG
    CGATCTCACC GCCAACTGAT AGACTGCATC CGCAGCAAGG ACACAGATGC TCTCATAGAT
    GCCATTGATA CAGGTGCTTT TGAAGTAAAT TTTATGGATG ATGTTGGCCA GACTCTTCTG
    AATTGGGCCT CTGCTTTTGG CACACAGGAA ATGGTTGAAT TCCTGTGTGA GCGAGGTGCC
    GATGTAAATA GAGGGCAGAG GTCATCTTCA CTGCATTATG CTGCTTGCTT CGGAAGGCCT
    CAGGTGGCAA AGACGTTGCT TCGCCATGGA GCCAACCCAG ATTTAAGAGA TGAGGATGGA
    AAAACACCTT TGGATAAAGC CCGGGAGAGA GGACACAGCG AAGTGGTGGC CATTTTGCAG
    TCCCCAGGGG ACTGGATGTG CCCCGTAAAC AAAGGAGATG AAAAGAAAAA GAAAGACTCT
    AACCGGGAAG AGGAAGAATG CAATGAGCCA AAGGGAGACC CAGAAATGGC CCCCATCTAC
    CTAAAGAGGC TGCTGCCTGT CTTTGCACAG ACCTTTCAGC AAACCATGCT TCCCTCTATC
    AGGAAAGCAA GCTTAGCACT TATTCGGAAG ATGATCCATT TCTGCTCAGA AGCACTGCTT
    AAGGAAGTTT GTCACTCAGA CGCTGGACAC AATTTGCCCA CTGTTCTTGT TGAGATAACA
    GCCACTGTTT TAGACCAGGA GGACGATGAT GATGGTCACC TTCTGGCACT TCAAATCATT
    AGGGATTTGG TGGACAAAGG TGATGACCTT TTTCTTGATC AATTGGCCCG GCTTGGTGTT
    ATAAGCAAGG TTTCTACACT AGCTGGACCT ACATCAGATG ATGAGAATGA GGAGGATAGC
    AAGCCAGAGA AGGAGGATGA GCCACAGGAA GATGCCAAGG AGTTGCAACA GGGCCGGCCC
    TACCATTGGC GGGACTGGTC TGTTATTCGC GGTAGGGACT GTTTGTACAT CTGGAGTGAT
    GCTGCAGCTC TGGAGCTCTC TAATGGTAGC AATGGCTGGT TCCGCTTTAT CCTAGATGGG
    AAACTGGCAA CCATGTATTC AAGTGGAAGC CCAGAGGGGG GTTCTGATAG CTCAGAAAGT
    AGAAGTGAAT TTTTAGAAAA GTTACAGCGG GCCCGCAGCC AGGTCAAACC ATCCACTTCT
    AGCCAGCCTA TCTTGTCCAC TCCTGGTCCA GGAAAGCTAA CAGTAGGCAA CTGGTCTCTC
    ACTTGTCTGA AAGATGGTGA GATTGCAATC CACAACTCGG ACGGGCAGCA GGCTACAATA
    CTGAAGGAGG ATCTACCAGG CTTTGTGTTT GAGTCAAACA GGGGTACCAA GCACTCCTTC
    ACGGCAGAAA CATCACTAGG ATCTGAATTT GTAACTGGCT GGACAGGAAA GAGAGGCAGA
    AAGCTGAAGT CTAAGTTGGA GAAAACTAAA CAGAAGGTAC GGACAATGGC AAGAGATTTG
    TATGATGATC ATTTTAAGGC AGTGGAAAGC ATGCCCCGAG GAGTGGTAGT GACGCTCCGA
    AATATTGCAA CACAGCTGGA ATCTGCTTGG GAACTTCATA CAAACAGACA GTGTATTGAA
    GGAGAGAACA CATGGAGAGA TCTGATGAAA ACTGCCCTGG AAAACCTGAT TGTGCTACTG
    AAGGATGAGA ATACTATTTC CCCTTATGAG ATGTGCAGCA GTGGACTGGT GCAGGCTTTG
    CTTACGGTGC TCAATAATAA TGAAGACTGT GATATAAAAC AAGACTGTGG GCAGCTGGTG
    GAGAGACTTA ATGTATTTAA AACTGCCTTC AGCGAAAATG AAGATGATGA AAGCCGCCCT
    GCCGTTGCCC TCGTCCGGAA GTTGATTGCT GTGCTTGAAT CAATAGAACG GCTGCCCCTT
    CACCTGTATG ACACCCCTGG ATCTAGCTAT AACTTGCAGA TCCTGACCCG TAGGCTTCGG
    TTTCGGCTGG AGCGTGCACC TGGAGAAACG TCTCTAATCG ACCGTACCGG TCGGATGCTG
    AAAATGGAGC CACTGGCAAC TGTAGAGTCC TTGGAGCAGT ATTTACTGAA AATGGTGGCT
    AAGCAGTGGT ACGACTTTGA CCGTTCGTCC TTTGTGTTTG TGCGCAAATT GCGCGAGGGC
    CAGAGTTGTG TGTTCCGCCA CCAGCATGAC TTTGATGATA ATGGAATCAT GTACTGGATT
    GGCACCAATG CAAAGACAGC TTATGAGTGG GTCAACCCTG CTGCTTACGG ACTAGTAGTT
    GTTACATCTT CTGAAGGCCG CAATTTGCCA TATGGCCGCC TTGAGGATAT CCTGAGCCGT
    GATAGCTCTG CCCTTAATTG CCACACTAAT GATGATAAGA GTGCCTGGTT TGCCATTGAC
    CTTGGCTTGT GGGTTGTGCC TTCTGCCTAT ACACTGCGTC ATGCTCGTGG ATATGGCCGT
    TCCGCTCTTC GCAATTGGGT CTTCCAGGTG TCTAAAGATG GTCAGAACTG GACCACCCTC
    TACACCCACA TGGATGACTG CAGCCTGAAT GAACCTGGCT CCACAGCCAC ATGGCCTCTG
    GACCCTGCGA GAGAAGAAAA GCAGGGTTGG CGTCATGTCC GGATAAAGCA GACGGGAAAG
    AATGCCAGTG GCCAGACCCA CTACCTCTCA CTTTCTGGCT TTGAGCTTTA TGGCAATGTG
    ACTGGGGTGT GTGAAGACCA ACTTGGTAAA GCTGCCAAAG AGGCAGAAGC CAACTTAAGG
    CGCCAGAGGC GGCTGGTACG CTCTCAGGTG CTAAAATATA TGGTGCCTGG AGCCCGTGTT
    ATTCGGGGCA TTGATTGGAA GTGGCGAGAC CAAGATGGCA GTGCTCAAGG AGAAGGCACT
    GTTACAGGAG AACTGCACAA TGGCTGGATT GATGTCATAT GGGATGCCGG TGGCTCTAAC
    TCTTACCGCA TGGGCGCGGA AGGAAAATTT GACCTGAAGC TGGCCCCGGG TTATGACCCA
    GAGTCTGCGG TGTCACCCAA ACCTGTTTCA TCCACTGTTG CAGGAACACC GCCCTCGTGG
    AGCAGCCTGG TGAAAAACAA CTGTCCTGAC AAAGCCCCTC CCTCTTCCTC ATCCTCTTGT
    GTGGTCGTTG GAAGCGTGGC GGGCTCAGGC AGCCGCAAAG GAAGCAGCAG CTCGGTCTGT
    AGCGTGGCAA GCAGCAGCGA CGTAAGCCTA AGCTGTGCAA AGACAGAGAG GAGGGCAGAG
    GAGCAAGTCT CCGACATTCA CCATGATCCC ATCCTGCTTC TGTCCTCTAA CCAAGCAGCC
    TCGGGTTCCT CCACCTGCCC CCCTGGAGGA GAGACTGTGG GTGAAGGTGG AGATAGAAAG
    GCAGGAGAAG CACCAGCAAT CTCAATGGGT ATGGTGAGCA TTAGCTCCCC TGATGTCAGT
    TCCGTTTCCG AACTCAGCAA CAAGGAAGTG GCAGTTCCAC GACCACTGGG ATCTTCTGCC
    AGCAACAGAT TGTCTGTCAG TTCACTGTTG GCAGCTGGGG CACCCATGAG TTCTAGCGCC
    AGTGTACCAA ACTTGTCCTC CAGGGAAACG TCCAGTCTGG AGTCCTTTGT AAGGAGAGTG
    GCAAATATCG CCAGGACAAA TGCAACCAAC AACATGAACC TGAGTCGTAG CAGCAGTGAT
    AACAATACCA ACACTCTGGG GAGGAATGCA GTCAGCTCAG CCACTTCACC ACTTATGGGA
    GCTCAGAGCT TTCCCAACCT GACGACAACA GGAACCACAT CCACTGTTAC AATGTCTACA
    TCCAGTGTCA CCAGCAGTAA TGTGGCAACA GCCACTACAG GGTTGTCTGT AGGTCAGTCC
    CTCAGTAACA CCCTGACTAC AAGCCTAACC TCGACATCCA GTGAGAGTGA CACTGGGCAG
    GAGGCAGAAT ACTCCCTGTA TGACTTTCTT GATAGCTGCC GTGCCAGCAC TTTGCTTGCT
    GAACTTGATG ATGACGAGGA TCTCCCTGAG CCCGATGAGG AAGATGATGA AAACGAAGAT
    GATAATCAGG AAGAGCAAGA GTATGAAGAA GTTATGGAGG AGGAAGAGTA TGAGACCAAA
    GGTGGCCGAC GCAGGACCTG GGATGATGAT TATGTGCTGA AGAGGCAGTT TTCTGCTTTG
    GTCCCGGCTT TTGACCCACG CCCAGGGAGA ACTAATGTTC AGCAGACCAC TGATCTTGAA
    ATTCCTGCTC CAGGCACGCC CCACTCTGAG CTACTGGAGG AGGTAGAATG TGCCCCAGCT
    CCACGATTAG CACTCACCCT AAAGGTGACA GGCCTTGGGA GTGGGAGAGA GGTGGAGCTC
    CCCCTGAACA ATTTCCGTTC CACCATATTT TTCTATGTAC AGAGACTGCT GCAACTCTCC
    TGCAATGGAG CCATCAAAAC TGACAAGCTG AGAAGGATTT GGGAGCCCAC CTACACGATT
    ATGTACAGAG AGATGAAGGA TTCAGATAAG CAAAAAGAAT GTGGCAGACT GGGCTGTTGG
    TCTGTAGAGC ATGTGGAACA ATCCCTAGGC ACAGATGCCT TGCCAAAGAA TGACCTGATC
    ACTTACCTGC AGCGAAATGC AGATCCAGGG TTCTTGCGAC GCTGGAAACT GACAGGAACC
    AACAAAAGCA TTAGGAAAAA CCGCAACTGT TCCCAGCTTA TTGCTGCTTA CAAGGACTTC
    TGTGAGAATG GCTGCAAGTC TCTCTCAATG CCAGCTGCTC TTGCCACACT CCAGAGTGCT
    GATATCCTTA GTCATTCCCG AGAGCAGGCA CAAGCCAAAG CGGGCAGCAG CCAAAACTCC
    TGCGGGGTAG AAGATGTTTT GCAGCTCCTG CGAATTCTCT TTATTGTAGC CAGTGACCCA
    TATTCTGCCA GGACCCCACA GGAAGATGGA GAAGATATGC TCCTTTTCAG TGTTCCTCCA
    GAGGAGTTTA CTAGCAAGAA AATCACCACA AAGATTGTTC AGCAAATCGA GGAACCTTTA
    GCTTTAGCAA GTGGAGCTTT GCCAGACTGG TGTGAACAGC TGACCAGCAA ATGTCCCTTC
    TTAATACCCT TTGAAACCCG GCAGCTTTAC TTCACATGCA CAGCATTTGG TGCCTCAAGG
    GCTATTGTGT GGCTGCAGAA CAGGCGGGAA GCCACAGTTG AGAGGAGCAG AACAGCAAGT
    GCAGTGCGCA GAGACGACCC AGGGGAGTTC AGAGTGGGCC GTCTAAAACA CGAACGTGTG
    AAGGTCCCAC GAGGGGAGTC TTTGATGGAG TGGGCAGAGA ATGTTATGCA AATCCACGCA
    GACCGAAAAT CTGTGCTTGA GGTGGAGTTT CTGGGAGAAG AAGGAACAGG CTTGGGCCCT
    ACCCTTGAGT TTTATGCCCT GGTGGCAGCA GAGTTCCAGA AAACAGATTT GGGGATCTGG
    TTATGTGATG ATGACTTCCC AGATGATGAG TCTCGGCAGG TGGATCTAGG TGGTGGTTTG
    AAGCCCCCTG GTTACTATGT GCAGCGTTCC TGTGGCCTCT TCATTGCACC CTACCCACAG
    GATAGTGAGG AACTAGACCG AGTGACCAGA CTTTGCCATT TCCTTGGTGT TTTCCTTGCA
    AAGTGCATCC AGGACAATAG ATTAGTGGAC TTGCCCATTT CAAAGCCGTT TTTTAAGCTT
    ATGTGTATGG GTGACATTAA GAGCAACATG AGCAAGCTTT TGTATGCCTC TCGGGGAGAG
    GAGAGTGAGC ACTGCACGGA GAGCCAGTCG GAGGCCTCTA CTGAGGATGG GCATGATGCC
    CTTTCAGTAG GAAGCTTTGA GGAAGACTGC AAATCTGAGT TCATCCTAGA CCCACCCAAA
    CCAAAGCCAC CAGCCTGGTT TCAGGGTATT TTAACTTGGG AGGACTTTGA GCTAATAAAC
    CCACATCGTG CTCGCTTCCT TAGGGACATT AGGGAGTTGG CTGTAAAGAG GAGACAGATT
    CTGGGGAACC GTTGCTTATC TGAGGATGAG AAGAACACTC AACTTCAGGA ACTTATGCTA
    AAGAATCCTT CTGGATCTGG GCCTCCAGTC AGCATTGAGG ATTTGGGACT CAATTTCCAG
    TTCTGTCCAT CTTCCCGGGT TTATGGCTTC TCTGCAGTCG ATCTAAGGCC AAATGGAGAG
    GATGAGATGG TGACGATAGA CAACGCTGAG GAGTATGTTG ATCTTATGTT TGATTTCTGC
    ATGCAGACTG GTGTACAAAA GCAAATGGAA GCATTTAGAA GTGGCTTTAA TAAAGTGTTC
    CCTATGGAGA AACTGGGCTC CTTCAGCCCC GAGGAAGTCC AAATGATACT GTGTGGAAAC
    CAGTCTCCCT CTTGGTCCGC TGAGGACATC ATCAATTACA CAGAACCAAA GCTAGGATAT
    ACCCGAGAGA GTCCTGGGTT CCTTCGATTC GTGAGAGTCC TATGTGGCAT GTCGTCAGAT
    GAGAGGAAAG CGTTCCTGCA GTTTACAACG GGGTGCTCCA CATTGCCTCC AGGAGGACTG
    GCCAATCTGC ATCCGCGTCT CACAGTCGTG CGCAAGGTTG ATGCCACGGA TGCCAGCTAC
    CCATCTGTAA ATACATGTGT GCATTACCTG AAGCTGCCGG AGTATTCCTC CGAGGAAATC
    ATGAGAGACC GCCTGCTTGC TGCTACCATG GAGAAAGGAT TCCATCTCAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012823551.1
    CDS315..7988
    Translation

    Target ORF information:

    RefSeq Version XM_012968097.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis HECT domain containing E3 ubiquitin protein ligase 1 (hectd1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012968097.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGCTGACG TAGATCCAGA CACACTACTG GAATGGCTGC AGATGGGGCA GGGGGACGAG 
    AGAGACATGC AGCTCATTGC CTTGGAACAG CTCTGCATGC TGCTGCTCAT GTCGGATAAT
    GTGGATAGGT GTTTTGAAAC ATGCCCACCT CGCACCTTCC TGCCAGCCTT GTGTAAAATA
    TTCCTGGATG AAAGCGCCCC AGACAATGTG TTGGAAGTAA CAGCCCGTGC AATAACCTAT
    TACCTAGATG TGTCCGCCGA GTGCACGAGG CGTATTGTGG GAGTGGAAGG GGCTATCAAA
    GCTTTGTGCA ATAGGCTTGT GGTGGTGGAG CTCAACAACA GAACCAGTCG GGATTTGGCA
    GAACAGTGTG TCAAGGTGCT GGAGCTTATC TGTACACGTG AGTCTGGGGC TGTCTTTGAG
    GCAGGGGGCT TGAACTGCGT GTTGACATTC ATTCGGGACA GCGGACACCT AGTTCACAAG
    GATACGCTGC ATTCTGCAAT GGCTGTGGTG TCCCGACTGT GTGGTAAGAT GGAGCCACAG
    GATGCATCAT TAGAAACGTG TGTGGAGTCT CTCTCCAGCC TGCTGAAGCA CGAAGACCAT
    CAGGTATCAG ATGGAGCTCT CCGCTGCTTT GCTTCCCTTG CTGACCGCTT TACACGCCGT
    GGTGTAGATC CAGCTCCTCT TGCACAGCAT GGGCTCACTG AGGAACTGCT GTCCCGAATG
    GCAGCAGCCG GAGGCACTGT ATCAGGTCCA TCTTCAGCCT GTAAAACAGG CCGTGGCACC
    AGTGGGGGAC CTTCCACCTC TGGAGACTCA AAAATCAGCA ATCAGGTCTC CACTATAGTC
    AGCCTGTTAT CAACACTATG CCGTGGTTCT CCAGTCGTCA CACATGATTT ACTGCGGGCA
    GAACTCTTAG ACTCCATGGA AAGTGCTCTT CAAGGTGACG AGCGTTGTGT GCTGGATACC
    ATGCGCCTTG TAGACCTTCT TTTGGTGCTG CTTTTCGAAG GCCGCAAAGC TCTGCCTAAG
    TCTAGTGCTG GGTCCACAGG TCGTATTCCT GGGCTGCGGC GGCTTGACAG CTCAGGAGAG
    CGATCTCACC GCCAACTGAT AGACTGCATC CGCAGCAAGG ACACAGATGC TCTCATAGAT
    GCCATTGATA CAGGTGCTTT TGAAGTAAAT TTTATGGATG ATGTTGGCCA GACTCTTCTG
    AATTGGGCCT CTGCTTTTGG CACACAGGAA ATGGTTGAAT TCCTGTGTGA GCGAGGTGCC
    GATGTAAATA GAGGGCAGAG GTCATCTTCA CTGCATTATG CTGCTTGCTT CGGAAGGCCT
    CAGGTGGCAA AGACGTTGCT TCGCCATGGA GCCAACCCAG ATTTAAGAGA TGAGGATGGA
    AAAACACCTT TGGATAAAGC CCGGGAGAGA GGACACAGCG AAGTGGTGGC CATTTTGCAG
    TCCCCAGGGG ACTGGATGTG CCCCGTAAAC AAAGGAGATG AAAAGAAAAA GAAAGACTCT
    AACCGGGAAG AGGAAGAATG CAATGAGCCA AAGGGAGACC CAGAAATGGC CCCCATCTAC
    CTAAAGAGGC TGCTGCCTGT CTTTGCACAG ACCTTTCAGC AAACCATGCT TCCCTCTATC
    AGGAAAGCAA GCTTAGCACT TATTCGGAAG ATGATCCATT TCTGCTCAGA AGCACTGCTT
    AAGGAAGTTT GTCACTCAGA CGCTGGACAC AATTTGCCCA CTGTTCTTGT TGAGATAACA
    GCCACTGTTT TAGACCAGGA GGACGATGAT GATGGTCACC TTCTGGCACT TCAAATCATT
    AGGGATTTGG TGGACAAAGG TGATGACCTT TTTCTTGATC AATTGGCCCG GCTTGGTGTT
    ATAAGCAAGG TTTCTACACT AGCTGGACCT ACATCAGATG ATGAGAATGA GGAGGATAGC
    AAGCCAGAGA AGGAGGATGA GCCACAGGAA GATGCCAAGG AGTTGCAACA GGGCCGGCCC
    TACCATTGGC GGGACTGGTC TGTTATTCGC GGTAGGGACT GTTTGTACAT CTGGAGTGAT
    GCTGCAGCTC TGGAGCTCTC TAATGGTAGC AATGGCTGGT TCCGCTTTAT CCTAGATGGG
    AAACTGGCAA CCATGTATTC AAGTGGAAGC CCAGAGGGGG GTTCTGATAG CTCAGAAAGT
    AGAAGTGAAT TTTTAGAAAA GTTACAGCGG GCCCGCAGCC AGGTCAAACC ATCCACTTCT
    AGCCAGCCTA TCTTGTCCAC TCCTGGTCCA GGAAAGCTAA CAGTAGGCAA CTGGTCTCTC
    ACTTGTCTGA AAGATGGTGA GATTGCAATC CACAACTCGG ACGGGCAGCA GGCTACAATA
    CTGAAGGAGG ATCTACCAGG CTTTGTGTTT GAGTCAAACA GGGGTACCAA GCACTCCTTC
    ACGGCAGAAA CATCACTAGG ATCTGAATTT GTAACTGGCT GGACAGGAAA GAGAGGCAGA
    AAGCTGAAGT CTAAGTTGGA GAAAACTAAA CAGAAGGTAC GGACAATGGC AAGAGATTTG
    TATGATGATC ATTTTAAGGC AGTGGAAAGC ATGCCCCGAG GAGTGGTAGT GACGCTCCGA
    AATATTGCAA CACAGCTGGA ATCTGCTTGG GAACTTCATA CAAACAGACA GTGTATTGAA
    GGAGAGAACA CATGGAGAGA TCTGATGAAA ACTGCCCTGG AAAACCTGAT TGTGCTACTG
    AAGGATGAGA ATACTATTTC CCCTTATGAG ATGTGCAGCA GTGGACTGGT GCAGGCTTTG
    CTTACGGTGC TCAATAATAA TGAAGACTGT GATATAAAAC AAGACTGTGG GCAGCTGGTG
    GAGAGACTTA ATGTATTTAA AACTGCCTTC AGCGAAAATG AAGATGATGA AAGCCGCCCT
    GCCGTTGCCC TCGTCCGGAA GTTGATTGCT GTGCTTGAAT CAATAGAACG GCTGCCCCTT
    CACCTGTATG ACACCCCTGG ATCTAGCTAT AACTTGCAGA TCCTGACCCG TAGGCTTCGG
    TTTCGGCTGG AGCGTGCACC TGGAGAAACG TCTCTAATCG ACCGTACCGG TCGGATGCTG
    AAAATGGAGC CACTGGCAAC TGTAGAGTCC TTGGAGCAGT ATTTACTGAA AATGGTGGCT
    AAGCAGTGGT ACGACTTTGA CCGTTCGTCC TTTGTGTTTG TGCGCAAATT GCGCGAGGGC
    CAGAGTTGTG TGTTCCGCCA CCAGCATGAC TTTGATGATA ATGGAATCAT GTACTGGATT
    GGCACCAATG CAAAGACAGC TTATGAGTGG GTCAACCCTG CTGCTTACGG ACTAGTAGTT
    GTTACATCTT CTGAAGGCCG CAATTTGCCA TATGGCCGCC TTGAGGATAT CCTGAGCCGT
    GATAGCTCTG CCCTTAATTG CCACACTAAT GATGATAAGA GTGCCTGGTT TGCCATTGAC
    CTTGGCTTGT GGGTTGTGCC TTCTGCCTAT ACACTGCGTC ATGCTCGTGG ATATGGCCGT
    TCCGCTCTTC GCAATTGGGT CTTCCAGGTG TCTAAAGATG GTCAGAACTG GACCACCCTC
    TACACCCACA TGGATGACTG CAGCCTGAAT GAACCTGGCT CCACAGCCAC ATGGCCTCTG
    GACCCTGCGA GAGAAGAAAA GCAGGGTTGG CGTCATGTCC GGATAAAGCA GACGGGAAAG
    AATGCCAGTG GCCAGACCCA CTACCTCTCA CTTTCTGGCT TTGAGCTTTA TGGCAATGTG
    ACTGGGGTGT GTGAAGACCA ACTTGGTAAA GCTGCCAAAG AGGCAGAAGC CAACTTAAGG
    CGCCAGAGGC GGCTGGTACG CTCTCAGGTG CTAAAATATA TGGTGCCTGG AGCCCGTGTT
    ATTCGGGGCA TTGATTGGAA GTGGCGAGAC CAAGATGGCA GTGCTCAAGG AGAAGGCACT
    GTTACAGGAG AACTGCACAA TGGCTGGATT GATGTCATAT GGGATGCCGG TGGCTCTAAC
    TCTTACCGCA TGGGCGCGGA AGGAAAATTT GACCTGAAGC TGGCCCCGGG TTATGACCCA
    GAGTCTGCGG TGTCACCCAA ACCTGTTTCA TCCACTGTTG CAGGAACACC GCCCTCGTGG
    AGCAGCCTGG TGAAAAACAA CTGTCCTGAC AAAGCCCCTC CCTCTTCCTC ATCCTCTTGT
    GTGGTCGTTG GAAGCGTGGC GGGCTCAGGC AGCCGCAAAG GAAGCAGCAG CTCGGTCTGT
    AGCGTGGCAA GCAGCAGCGA CGTAAGCCTA AGCTGTGCAA AGACAGAGAG GAGGGCAGAG
    GAGCAAGTCT CCGACATTCA CCATGATCCC ATCCTGCTTC TGTCCTCTAA CCAAGCAGCC
    TCGGGTTCCT CCACCTGCCC CCCTGGAGGA GAGACTGTGG GTGAAGGTGG AGATAGAAAG
    GCAGGAGAAG CACCAGCAAT CTCAATGGGT ATGGTGAGCA TTAGCTCCCC TGATGTCAGT
    TCCGTTTCCG AACTCAGCAA CAAGGAAGTG GCAGTTCCAC GACCACTGGG ATCTTCTGCC
    AGCAACAGAT TGTCTGTCAG TTCACTGTTG GCAGCTGGGG CACCCATGAG TTCTAGCGCC
    AGTGTACCAA ACTTGTCCTC CAGGGAAACG TCCAGTCTGG AGTCCTTTGT AAGGAGAGTG
    GCAAATATCG CCAGGACAAA TGCAACCAAC AACATGAACC TGAGTCGTAG CAGCAGTGAT
    AACAATACCA ACACTCTGGG GAGGAATGCA GTCAGCTCAG CCACTTCACC ACTTATGGGA
    GCTCAGAGCT TTCCCAACCT GACGACAACA GGAACCACAT CCACTGTTAC AATGTCTACA
    TCCAGTGTCA CCAGCAGTAA TGTGGCAACA GCCACTACAG GGTTGTCTGT AGGTCAGTCC
    CTCAGTAACA CCCTGACTAC AAGCCTAACC TCGACATCCA GTGAGAGTGA CACTGGGCAG
    GAGGCAGAAT ACTCCCTGTA TGACTTTCTT GATAGCTGCC GTGCCAGCAC TTTGCTTGCT
    GAACTTGATG ATGACGAGGA TCTCCCTGAG CCCGATGAGG AAGATGATGA AAACGAAGAT
    GATAATCAGG AAGAGCAAGA GTATGAAGAA GTTATGGAGG AGGAAGAGTA TGAGACCAAA
    GGTGGCCGAC GCAGGACCTG GGATGATGAT TATGTGCTGA AGAGGCAGTT TTCTGCTTTG
    GTCCCGGCTT TTGACCCACG CCCAGGGAGA ACTAATGTTC AGCAGACCAC TGATCTTGAA
    ATTCCTGCTC CAGGCACGCC CCACTCTGAG CTACTGGAGG AGGTAGAATG TGCCCCAGCT
    CCACGATTAG CACTCACCCT AAAGGTGACA GGCCTTGGGA GTGGGAGAGA GGTGGAGCTC
    CCCCTGAACA ATTTCCGTTC CACCATATTT TTCTATGTAC AGAGACTGCT GCAACTCTCC
    TGCAATGGAG CCATCAAAAC TGACAAGCTG AGAAGGATTT GGGAGCCCAC CTACACGATT
    ATGTACAGAG AGATGAAGGA TTCAGATAAG CAAAAAGAAT GTGGCAGACT GGGCTGTTGG
    TCTGTAGAGC ATGTGGAACA ATCCCTAGGC ACAGATGCCT TGCCAAAGAA TGACCTGATC
    ACTTACCTGC AGCGAAATGC AGATCCAGGG TTCTTGCGAC GCTGGAAACT GACAGGAACC
    AACAAAAGCA TTAGGAAAAA CCGCAACTGT TCCCAGCTTA TTGCTGCTTA CAAGGACTTC
    TGTGAGAATG GCTGCAAGTC TCTCTCAATG CCAGCTGCTC TTGCCACACT CCAGAGTGCT
    GATATCCTTA GTCATTCCCG AGAGCAGGCA CAAGCCAAAG CGGGCAGCAG CCAAAACTCC
    TGCGGGGTAG AAGATGTTTT GCAGCTCCTG CGAATTCTCT TTATTGTAGC CAGTGACCCA
    TATTCTGCCA GGACCCCACA GGAAGATGGA GAAGATATGC TCCTTTTCAG TGTTCCTCCA
    GAGGAGTTTA CTAGCAAGAA AATCACCACA AAGATTGTTC AGCAAATCGA GGAACCTTTA
    GCTTTAGCAA GTGGAGCTTT GCCAGACTGG TGTGAACAGC TGACCAGCAA ATGTCCCTTC
    TTAATACCCT TTGAAACCCG GCAGCTTTAC TTCACATGCA CAGCATTTGG TGCCTCAAGG
    GCTATTGTGT GGCTGCAGAA CAGGCGGGAA GCCACAGTTG AGAGGAGCAG AACAGCAAGT
    GCAGTGCGCA GAGACGACCC AGGGGAGTTC AGAGTGGGCC GTCTAAAACA CGAACGTGTG
    AAGGTCCCAC GAGGGGAGTC TTTGATGGAG TGGGCAGAGA ATGTTATGCA AATCCACGCA
    GACCGAAAAT CTGTGCTTGA GGTGGAGTTT CTGGGAGAAG AAGGAACAGG CTTGGGCCCT
    ACCCTTGAGT TTTATGCCCT GGTGGCAGCA GAGTTCCAGA AAACAGATTT GGGGATCTGG
    TTATGTGATG ATGACTTCCC AGATGATGAG TCTCGGCAGG TGGATCTAGG TGGTGGTTTG
    AAGCCCCCTG GTTACTATGT GCAGCGTTCC TGTGGCCTCT TCATTGCACC CTACCCACAG
    GATAGTGAGG AACTAGACCG AGTGACCAGA CTTTGCCATT TCCTTGGTGT TTTCCTTGCA
    AAGTGCATCC AGGACAATAG ATTAGTGGAC TTGCCCATTT CAAAGCCGTT TTTTAAGCTT
    ATGTGTATGG GTGACATTAA GAGCAACATG AGCAAGCTTT TGTATGCCTC TCGGGGAGAG
    GAGAGTGAGC ACTGCACGGA GAGCCAGTCG GAGGCCTCTA CTGAGGATGG GCATGATGCC
    CTTTCAGTAG GAAGCTTTGA GGAAGACTGC AAATCTGAGT TCATCCTAGA CCCACCCAAA
    CCAAAGCCAC CAGCCTGGTT TCAGGGTATT TTAACTTGGG AGGACTTTGA GCTAATAAAC
    CCACATCGTG CTCGCTTCCT TAGGGACATT AGGGAGTTGG CTGTAAAGAG GAGACAGATT
    CTGGGGAACC GTTGCTTATC TGAGGATGAG AAGAACACTC AACTTCAGGA ACTTATGCTA
    AAGAATCCTT CTGGATCTGG GCCTCCAGTC AGCATTGAGG ATTTGGGACT CAATTTCCAG
    TTCTGTCCAT CTTCCCGGGT TTATGGCTTC TCTGCAGTCG ATCTAAGGCC AAATGGAGAG
    GATGAGATGG TGACGATAGA CAACGCTGAG GAGTATGTTG ATCTTATGTT TGATTTCTGC
    ATGCAGACTG GTGTACAAAA GCAAATGGAA GCATTTAGAA GTGGCTTTAA TAAAGTGTTC
    CCTATGGAGA AACTGGGCTC CTTCAGCCCC GAGGAAGTCC AAATGATACT GTGTGGAAAC
    CAGTCTCCCT CTTGGTCCGC TGAGGACATC ATCAATTACA CAGAACCAAA GCTAGGATAT
    ACCCGAGAGA GTCCTGGGTT CCTTCGATTC GTGAGAGTCC TATGTGGCAT GTCGTCAGAT
    GAGAGGAAAG CGTTCCTGCA GTTTACAACG GGGTGCTCCA CATTGCCTCC AGGAGGACTG
    GCCAATCTGC ATCCGCGTCT CACAGTCGTG CGCAAGGTTG ATGCCACGGA TGCCAGCTAC
    CCATCTGTAA ATACATGTGT GCATTACCTG AAGCTGCCGG AGTATTCCTC CGAGGAAATC
    ATGAGAGACC GCCTGCTTGC TGCTACCATG GAGAAAGGAT TCCATCTCAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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