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slc9a8 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Summary

Gene Symbol slc9a8
Entrez Gene ID 100216016
Full Name solute carrier family 9 member 8
General protein information
Preferred Names

solute carrier family 9 member 8

Names

sodium/hydrogen exchanger 8
solute carrier family 9 (sodium/hydrogen exchanger), member 8
solute carrier family 9, subfamily A (NHE8, cation proton antiporter 8), member 8
Gene Type protein-coding
Organism Xenopus tropicalis(tropical clawed frog)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_012971560.2 XP_012827014.1 sodium/hydrogen exchanger 8 isoform X1
XM_018097676.1 XP_017953165.1 sodium/hydrogen exchanger 8 isoform X1
NM_001197262.1 NP_001184191.1 sodium/hydrogen exchanger 8

Pathways from BioSystems


Homologies


Bos taurus (cattle) SLC9A8 XP_005215117.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP009446 XP_307859.4
Homo sapiens (human) SLC9A8 NP_001247420.1
Canis lupus familiaris (dog) SLC9A8 XP_005635223.1
Drosophila melanogaster (fruit fly) Nhe1 NP_608491.3
Arabidopsis thaliana (thale cress) NHX6 NP_178079.2
Arabidopsis thaliana (thale cress) NHX5 NP_175839.2
Mus musculus (house mouse) Slc9a8 NP_683731.1
Rattus norvegicus (Norway rat) Slc9a8 NP_001020452.1
Oryza sativa (rice) Os09g0286400 NP_001062780.1
Xenopus tropicalis (tropical clawed frog) slc9a8 NP_001184191.1
Pan troglodytes (chimpanzee) SLC9A8 XP_514716.3
Gallus gallus (chicken) SLC9A8 NP_001034364.1
Danio rerio (zebrafish) slc9a8 NP_001008586.1
Caenorhabditis elegans (roundworm) nhx-8 NP_001021729.1

Gene Ontology


Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following slc9a8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the slc9a8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa20292 NM_001197262.1
Latest version!		 Xenopus tropicalis solute carrier family 9 member 8 (slc9a8), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $342.30
$489.00
OXa32516 XM_018097676.1
Latest version!		 Xenopus tropicalis solute carrier family 9, subfamily A (NHE8, cation proton antiporter 8), member 8 (slc9a8), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $342.30
$489.00
OXa32516 XM_012971560.2
Latest version!		 Xenopus tropicalis solute carrier family 9, subfamily A (NHE8, cation proton antiporter 8), member 8 (slc9a8), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $342.30
$489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OXa20292
Clone ID Related Accession (Same CDS sequence) NM_001197262.1
Accession Version NM_001197262.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1731bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-06-14
Organism Xenopus tropicalis(tropical clawed frog)
Product sodium/hydrogen exchanger 8
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL969787.2, EL803907.1, CX443172.1, AAMC03037109.1 and AAMC03037108.1. On or before Oct 17, 2010 this sequence version replaced XM_002933155.1, XM_002933156.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1025683, SAMEA1025684 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGCTGAAG CAGACTTTCA CAATGTCAGC CATGGAGAGT TTAACACCAC CTTGTTCACC 
ACCCTCGCAA TTTCGACAAA GCTTGTGCTT CCTACTCCAG AAAAGCCAAT TATACCAGTA 
CAGACTGGGG TCCAAGCTCA GCAGGAGGAA CAGTCCAGTG GCATGTCTAT ATTCTTTAGC 
CTTTTAGTTT TAGGCATTTG CATCATATTG GTGCATCTTC TGATAAAGTA CAGACTCCAT 
TTCCTGCCGG AAAGTGTTGC AGTCGTTTCA TTAGGTATTT TAATGGGAGC AGTGATAAAG 
ATCATAGAGT CCCAGAAACT AGCTAATTGG AAGGAAGAGG AAATGTTCCG TCCCAACACG 
TTCTTTCTGG TTCTCCTCCC CCCTATTATA TTTGAGTCTG GTTATTCCCT GCACAAGGGC 
AACTTCTTTC AGAACATAGG ATCCATCACC CTATTCGCAG TGTTTGGCAC AGCCATTTCT 
GCCTTTATTG TTGGTGGTGG GATTTACTTT TTAGGACAGG CTGATGTCAT CTACAAACTC 
ACAATGACAG ACAGCTTTGC TTTTGGCTCT TTAATATCGG CTGTGGACCC CGTAGCCACC 
ATTGCCATAT TCAATGCCCT TAATGTGGAT CCTGTACTCA ACATGTTGGT GTTTGGGGAA 
AGCATTCTCA ATGATGCAGT GTCCATCGTA CTGACCAACA CAGCGGAAGG TCTCAACAGA 
GGCAATATGG CTGATGTGAG CAGCTGGCAG ACTTTCCTAC AGGCCTTGGC ATATTTCCTC 
AAGATGTTTT TTGGTTCGGC GGCCCTCGGA ACACTGACCG GTCTGATTTC CGCATTAGTT 
TTAAAGCATC TGGATTTACG GAAGACACCT TCCTTGGAGT TTGGAATGAT GATCATCTTT 
GCTTACCTCC CCTATGGACT CTCAGAGGGC ATCTCTCTGT CTGGTATCAT GGCAATCTTG 
TTCTCTGGGA TAGTGATGTC CCATTACACA CATCACAACC TGTCCCCAGT CACACAGATA 
CTCATGCAAC AAACTCTGCG CACCGTGGCC TTCATGTGCG AAACCTGCGT TTTCGCTTTT 
CTCGGACTGT CGATTTTCAG TTTTCCTCAC AAGTTTGAAA TATCATTTGT CATCTGGTGC 
ATTGTTCTGG TCTTGCTGGG AAGAGCCGTC AACATATTCC CTCTGTCCTA CCTACTCAAC 
TTTTTCCGGG ACCATAAGAT CACCCCAAAA ATGATGTATA TCATGTGGTT CAGTGGCTTG 
CGAGGAGCCA TTCCATATGC CCTGAGCCTT CACTTGGAGC TGGAACCGAT TGAGAAACGC 
CAGCTGATAG GAACCACCAC TATAATTATC GTGCTCTTCA CAATTCTGAT TTTGGGAGGG 
GGCACCATGC CCCTCATTCG CCTGATCGAC ATCGAGGACT CCAAAGCACG CCGAAGGAGC 
AAAAAAGATG TGAACCTCAG TAAGACGGAA AAAATGGGTA ACACCATTGA GTCTGAGCAT 
TTGTCAGAGC TGACAGAAGA GGAATATGAG GCCCAGTACA TCAGACAACA CAACCTTAAA 
GGGTTTATGT GGCTGGATGC AAAGTATTTA AACCCTTTCT TTACTCGGAG GCTGACCCGG 
GAGGACCTTC ATCACGGGCG CATACAGATG AAGACCCTGA CCAATAAGTG GTATGAAGAG 
GTGCGCCAAG GGCCTTCAGG GTCCGAGGAT GATGAGCAGG AGCTTCTATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001184191.1
CDS21..1751
Translation

Target ORF information:

RefSeq Version NM_001197262.1
Organism Xenopus tropicalis(tropical clawed frog)
Definition Xenopus tropicalis solute carrier family 9 member 8 (slc9a8), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001197262.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGCTGAAG CAGACTTTCA CAATGTCAGC CATGGAGAGT TTAACACCAC CTTGTTCACC 
ACCCTCGCAA TTTCGACAAA GCTTGTGCTT CCTACTCCAG AAAAGCCAAT TATACCAGTA 
CAGACTGGGG TCCAAGCTCA GCAGGAGGAA CAGTCCAGTG GCATGTCTAT ATTCTTTAGC 
CTTTTAGTTT TAGGCATTTG CATCATATTG GTGCATCTTC TGATAAAGTA CAGACTCCAT 
TTCCTGCCGG AAAGTGTTGC AGTCGTTTCA TTAGGTATTT TAATGGGAGC AGTGATAAAG 
ATCATAGAGT CCCAGAAACT AGCTAATTGG AAGGAAGAGG AAATGTTCCG TCCCAACACG 
TTCTTTCTGG TTCTCCTCCC CCCTATTATA TTTGAGTCTG GTTATTCCCT GCACAAGGGC 
AACTTCTTTC AGAACATAGG ATCCATCACC CTATTCGCAG TGTTTGGCAC AGCCATTTCT 
GCCTTTATTG TTGGTGGTGG GATTTACTTT TTAGGACAGG CTGATGTCAT CTACAAACTC 
ACAATGACAG ACAGCTTTGC TTTTGGCTCT TTAATATCGG CTGTGGACCC CGTAGCCACC 
ATTGCCATAT TCAATGCCCT TAATGTGGAT CCTGTACTCA ACATGTTGGT GTTTGGGGAA 
AGCATTCTCA ATGATGCAGT GTCCATCGTA CTGACCAACA CAGCGGAAGG TCTCAACAGA 
GGCAATATGG CTGATGTGAG CAGCTGGCAG ACTTTCCTAC AGGCCTTGGC ATATTTCCTC 
AAGATGTTTT TTGGTTCGGC GGCCCTCGGA ACACTGACCG GTCTGATTTC CGCATTAGTT 
TTAAAGCATC TGGATTTACG GAAGACACCT TCCTTGGAGT TTGGAATGAT GATCATCTTT 
GCTTACCTCC CCTATGGACT CTCAGAGGGC ATCTCTCTGT CTGGTATCAT GGCAATCTTG 
TTCTCTGGGA TAGTGATGTC CCATTACACA CATCACAACC TGTCCCCAGT CACACAGATA 
CTCATGCAAC AAACTCTGCG CACCGTGGCC TTCATGTGCG AAACCTGCGT TTTCGCTTTT 
CTCGGACTGT CGATTTTCAG TTTTCCTCAC AAGTTTGAAA TATCATTTGT CATCTGGTGC 
ATTGTTCTGG TCTTGCTGGG AAGAGCCGTC AACATATTCC CTCTGTCCTA CCTACTCAAC 
TTTTTCCGGG ACCATAAGAT CACCCCAAAA ATGATGTATA TCATGTGGTT CAGTGGCTTG 
CGAGGAGCCA TTCCATATGC CCTGAGCCTT CACTTGGAGC TGGAACCGAT TGAGAAACGC 
CAGCTGATAG GAACCACCAC TATAATTATC GTGCTCTTCA CAATTCTGAT TTTGGGAGGG 
GGCACCATGC CCCTCATTCG CCTGATCGAC ATCGAGGACT CCAAAGCACG CCGAAGGAGC 
AAAAAAGATG TGAACCTCAG TAAGACGGAA AAAATGGGTA ACACCATTGA GTCTGAGCAT 
TTGTCAGAGC TGACAGAAGA GGAATATGAG GCCCAGTACA TCAGACAACA CAACCTTAAA 
GGGTTTATGT GGCTGGATGC AAAGTATTTA AACCCTTTCT TTACTCGGAG GCTGACCCGG 
GAGGACCTTC ATCACGGGCG CATACAGATG AAGACCCTGA CCAATAAGTG GTATGAAGAG 
GTGCGCCAAG GGCCTTCAGG GTCCGAGGAT GATGAGCAGG AGCTTCTATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OXa32516
Clone ID Related Accession (Same CDS sequence) XM_012971560.2 , XM_018097676.1
Accession Version XM_012971560.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1569bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-09-07
Organism Xenopus tropicalis(tropical clawed frog)
Product sodium/hydrogen exchanger 8 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012971560.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGTCTATAT TCTTTAGCCT TTTAGTTTTA GGCATTTGCA TCATATTGGT GCATCTTCTG 
ATAAAGTACA GACTCCATTT CCTGCCGGAA AGTGTTGCAG TCGTTTCATT AGGTATTTTA 
ATGGGAGCAG TGATAAAGAT CATAGAGTCC CAGAAACTAG CTAATTGGAA GGAAGAGGAA 
ATGTTCCGTC CCAACACGTT CTTTCTGGTT CTCCTCCCCC CTATTATATT TGAGTCTGGT 
TATTCCCTGC ACAAGGGCAA CTTCTTTCAG AACATAGGAT CCATCACCCT ATTCGCAGTG 
TTTGGCACAG CCATTTCTGC CTTTATTGTT GGTGGTGGGA TTTACTTTTT AGGACAGGCT 
GATGTCATCT ACAAACTCAC AATGACAGAC AGCTTTGCTT TTGGCTCTTT AATATCGGCT 
GTGGACCCCG TAGCCACCAT TGCCATATTC AATGCCCTTA ATGTGGATCC TGTACTCAAC 
ATGTTGGTGT TTGGGGAAAG CATTCTCAAT GATGCAGTGT CCATCGTACT GACCAACACA 
GCGGAAGGTC TCAACAGAGG CAATATGGCT GATGTGAGCA GCTGGCAGAC TTTCCTACAG 
GCCTTGGCAT ATTTCCTCAA GATGTTTTTT GGTTCGGCGG CCCTCGGAAC ACTGACCGGT 
CTGATTTCCG CATTAGTTTT AAAGCATCTG GATTTACGGA AGACACCTTC CTTGGAGTTT 
GGAATGATGA TCATCTTTGC TTACCTCCCC TATGGACTCT CAGAGGGCAT CTCTCTGTCT 
GGTATCATGG CAATCTTGTT CTCTGGGATA GTGATGTCCC ATTACACACA TCACAACCTG 
TCCCCAGTCA CACAGATACT CATGCAACAA ACTCTGCGCA CCGTGGCCTT CATGTGCGAA 
ACCTGCGTTT TCGCTTTTCT CGGACTGTCG ATTTTCAGTT TTCCTCACAA GTTTGAAATA 
TCATTTGTCA TCTGGTGCAT TGTTCTGGTC TTGCTGGGAA GAGCCGTCAA CATATTCCCT 
CTGTCCTACC TACTCAACTT TTTCCGGGAC CATAAGATCA CCCCAAAAAT GATGTATATC 
ATGTGGTTCA GTGGCTTGCG AGGAGCCATT CCGTATGCCC TGAGCCTTCA CTTGGAGCTG 
GAACCGATTG AGAAACGCCA GCTGATAGGA ACCACCACTA TAATTATCGT GCTCTTCACA 
ATTCTGATTT TGGGAGGGGG CACCATGCCC CTCATTCGCC TGATCGACAT CGAGGACTCC 
AAAGCACGCC GAAGGAGCAA AAAAGATGTG AACCTCAGTA AGACGGAAAA AATGGGTAAC 
ACCATTGAGT CTGAGCATTT GTCAGAGCTG ACAGAAGAGG AATATGAGGC CCAGTACATC 
AGACAACACA ACCTTAAAGG GTTTATGTGG CTGGATGCAA AGTATTTAAA CCCTTTCTTT 
ACTCGGAGGC TGACCCGGGA GGACCTTCAT CACGGGCGCA TACAGATGAA GACCCTGACC 
AATAAGTGGT ATGAAGAGGT GCGCCAAGGG CCTTCAGGGT CCGAGGATGA TGAGCAGGAG 
CTTCTATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_012827014.1
CDS383..1951
Translation

Target ORF information:

RefSeq Version XM_012971560.2
Organism Xenopus tropicalis(tropical clawed frog)
Definition Xenopus tropicalis solute carrier family 9, subfamily A (NHE8, cation proton antiporter 8), member 8 (slc9a8), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_012971560.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGTCTATAT TCTTTAGCCT TTTAGTTTTA GGCATTTGCA TCATATTGGT GCATCTTCTG 
ATAAAGTACA GACTCCATTT CCTGCCGGAA AGTGTTGCAG TCGTTTCATT AGGTATTTTA 
ATGGGAGCAG TGATAAAGAT CATAGAGTCC CAGAAACTAG CTAATTGGAA GGAAGAGGAA 
ATGTTCCGTC CCAACACGTT CTTTCTGGTT CTCCTCCCCC CTATTATATT TGAGTCTGGT 
TATTCCCTGC ACAAGGGCAA CTTCTTTCAG AACATAGGAT CCATCACCCT ATTCGCAGTG 
TTTGGCACAG CCATTTCTGC CTTTATTGTT GGTGGTGGGA TTTACTTTTT AGGACAGGCT 
GATGTCATCT ACAAACTCAC AATGACAGAC AGCTTTGCTT TTGGCTCTTT AATATCGGCT 
GTGGACCCCG TAGCCACCAT TGCCATATTC AATGCCCTTA ATGTGGATCC TGTACTCAAC 
ATGTTGGTGT TTGGGGAAAG CATTCTCAAT GATGCAGTGT CCATCGTACT GACCAACACA 
GCGGAAGGTC TCAACAGAGG CAATATGGCT GATGTGAGCA GCTGGCAGAC TTTCCTACAG 
GCCTTGGCAT ATTTCCTCAA GATGTTTTTT GGTTCGGCGG CCCTCGGAAC ACTGACCGGT 
CTGATTTCCG CATTAGTTTT AAAGCATCTG GATTTACGGA AGACACCTTC CTTGGAGTTT 
GGAATGATGA TCATCTTTGC TTACCTCCCC TATGGACTCT CAGAGGGCAT CTCTCTGTCT 
GGTATCATGG CAATCTTGTT CTCTGGGATA GTGATGTCCC ATTACACACA TCACAACCTG 
TCCCCAGTCA CACAGATACT CATGCAACAA ACTCTGCGCA CCGTGGCCTT CATGTGCGAA 
ACCTGCGTTT TCGCTTTTCT CGGACTGTCG ATTTTCAGTT TTCCTCACAA GTTTGAAATA 
TCATTTGTCA TCTGGTGCAT TGTTCTGGTC TTGCTGGGAA GAGCCGTCAA CATATTCCCT 
CTGTCCTACC TACTCAACTT TTTCCGGGAC CATAAGATCA CCCCAAAAAT GATGTATATC 
ATGTGGTTCA GTGGCTTGCG AGGAGCCATT CCGTATGCCC TGAGCCTTCA CTTGGAGCTG 
GAACCGATTG AGAAACGCCA GCTGATAGGA ACCACCACTA TAATTATCGT GCTCTTCACA 
ATTCTGATTT TGGGAGGGGG CACCATGCCC CTCATTCGCC TGATCGACAT CGAGGACTCC 
AAAGCACGCC GAAGGAGCAA AAAAGATGTG AACCTCAGTA AGACGGAAAA AATGGGTAAC 
ACCATTGAGT CTGAGCATTT GTCAGAGCTG ACAGAAGAGG AATATGAGGC CCAGTACATC 
AGACAACACA ACCTTAAAGG GTTTATGTGG CTGGATGCAA AGTATTTAAA CCCTTTCTTT 
ACTCGGAGGC TGACCCGGGA GGACCTTCAT CACGGGCGCA TACAGATGAA GACCCTGACC 
AATAAGTGGT ATGAAGAGGT GCGCCAAGGG CCTTCAGGGT CCGAGGATGA TGAGCAGGAG 
CTTCTATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OXa32516
Clone ID Related Accession (Same CDS sequence) XM_012971560.2 , XM_018097676.1
Accession Version XM_018097676.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1569bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-09-07
Organism Xenopus tropicalis(tropical clawed frog)
Product sodium/hydrogen exchanger 8 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGTCTATAT TCTTTAGCCT TTTAGTTTTA GGCATTTGCA TCATATTGGT GCATCTTCTG 
ATAAAGTACA GACTCCATTT CCTGCCGGAA AGTGTTGCAG TCGTTTCATT AGGTATTTTA 
ATGGGAGCAG TGATAAAGAT CATAGAGTCC CAGAAACTAG CTAATTGGAA GGAAGAGGAA 
ATGTTCCGTC CCAACACGTT CTTTCTGGTT CTCCTCCCCC CTATTATATT TGAGTCTGGT 
TATTCCCTGC ACAAGGGCAA CTTCTTTCAG AACATAGGAT CCATCACCCT ATTCGCAGTG 
TTTGGCACAG CCATTTCTGC CTTTATTGTT GGTGGTGGGA TTTACTTTTT AGGACAGGCT 
GATGTCATCT ACAAACTCAC AATGACAGAC AGCTTTGCTT TTGGCTCTTT AATATCGGCT 
GTGGACCCCG TAGCCACCAT TGCCATATTC AATGCCCTTA ATGTGGATCC TGTACTCAAC 
ATGTTGGTGT TTGGGGAAAG CATTCTCAAT GATGCAGTGT CCATCGTACT GACCAACACA 
GCGGAAGGTC TCAACAGAGG CAATATGGCT GATGTGAGCA GCTGGCAGAC TTTCCTACAG 
GCCTTGGCAT ATTTCCTCAA GATGTTTTTT GGTTCGGCGG CCCTCGGAAC ACTGACCGGT 
CTGATTTCCG CATTAGTTTT AAAGCATCTG GATTTACGGA AGACACCTTC CTTGGAGTTT 
GGAATGATGA TCATCTTTGC TTACCTCCCC TATGGACTCT CAGAGGGCAT CTCTCTGTCT 
GGTATCATGG CAATCTTGTT CTCTGGGATA GTGATGTCCC ATTACACACA TCACAACCTG 
TCCCCAGTCA CACAGATACT CATGCAACAA ACTCTGCGCA CCGTGGCCTT CATGTGCGAA 
ACCTGCGTTT TCGCTTTTCT CGGACTGTCG ATTTTCAGTT TTCCTCACAA GTTTGAAATA 
TCATTTGTCA TCTGGTGCAT TGTTCTGGTC TTGCTGGGAA GAGCCGTCAA CATATTCCCT 
CTGTCCTACC TACTCAACTT TTTCCGGGAC CATAAGATCA CCCCAAAAAT GATGTATATC 
ATGTGGTTCA GTGGCTTGCG AGGAGCCATT CCGTATGCCC TGAGCCTTCA CTTGGAGCTG 
GAACCGATTG AGAAACGCCA GCTGATAGGA ACCACCACTA TAATTATCGT GCTCTTCACA 
ATTCTGATTT TGGGAGGGGG CACCATGCCC CTCATTCGCC TGATCGACAT CGAGGACTCC 
AAAGCACGCC GAAGGAGCAA AAAAGATGTG AACCTCAGTA AGACGGAAAA AATGGGTAAC 
ACCATTGAGT CTGAGCATTT GTCAGAGCTG ACAGAAGAGG AATATGAGGC CCAGTACATC 
AGACAACACA ACCTTAAAGG GTTTATGTGG CTGGATGCAA AGTATTTAAA CCCTTTCTTT 
ACTCGGAGGC TGACCCGGGA GGACCTTCAT CACGGGCGCA TACAGATGAA GACCCTGACC 
AATAAGTGGT ATGAAGAGGT GCGCCAAGGG CCTTCAGGGT CCGAGGATGA TGAGCAGGAG 
CTTCTATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_017953165.1
CDS224..1792
Translation

Target ORF information:

RefSeq Version XM_018097676.1
Organism Xenopus tropicalis(tropical clawed frog)
Definition Xenopus tropicalis solute carrier family 9, subfamily A (NHE8, cation proton antiporter 8), member 8 (slc9a8), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_018097676.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGTCTATAT TCTTTAGCCT TTTAGTTTTA GGCATTTGCA TCATATTGGT GCATCTTCTG 
ATAAAGTACA GACTCCATTT CCTGCCGGAA AGTGTTGCAG TCGTTTCATT AGGTATTTTA 
ATGGGAGCAG TGATAAAGAT CATAGAGTCC CAGAAACTAG CTAATTGGAA GGAAGAGGAA 
ATGTTCCGTC CCAACACGTT CTTTCTGGTT CTCCTCCCCC CTATTATATT TGAGTCTGGT 
TATTCCCTGC ACAAGGGCAA CTTCTTTCAG AACATAGGAT CCATCACCCT ATTCGCAGTG 
TTTGGCACAG CCATTTCTGC CTTTATTGTT GGTGGTGGGA TTTACTTTTT AGGACAGGCT 
GATGTCATCT ACAAACTCAC AATGACAGAC AGCTTTGCTT TTGGCTCTTT AATATCGGCT 
GTGGACCCCG TAGCCACCAT TGCCATATTC AATGCCCTTA ATGTGGATCC TGTACTCAAC 
ATGTTGGTGT TTGGGGAAAG CATTCTCAAT GATGCAGTGT CCATCGTACT GACCAACACA 
GCGGAAGGTC TCAACAGAGG CAATATGGCT GATGTGAGCA GCTGGCAGAC TTTCCTACAG 
GCCTTGGCAT ATTTCCTCAA GATGTTTTTT GGTTCGGCGG CCCTCGGAAC ACTGACCGGT 
CTGATTTCCG CATTAGTTTT AAAGCATCTG GATTTACGGA AGACACCTTC CTTGGAGTTT 
GGAATGATGA TCATCTTTGC TTACCTCCCC TATGGACTCT CAGAGGGCAT CTCTCTGTCT 
GGTATCATGG CAATCTTGTT CTCTGGGATA GTGATGTCCC ATTACACACA TCACAACCTG 
TCCCCAGTCA CACAGATACT CATGCAACAA ACTCTGCGCA CCGTGGCCTT CATGTGCGAA 
ACCTGCGTTT TCGCTTTTCT CGGACTGTCG ATTTTCAGTT TTCCTCACAA GTTTGAAATA 
TCATTTGTCA TCTGGTGCAT TGTTCTGGTC TTGCTGGGAA GAGCCGTCAA CATATTCCCT 
CTGTCCTACC TACTCAACTT TTTCCGGGAC CATAAGATCA CCCCAAAAAT GATGTATATC 
ATGTGGTTCA GTGGCTTGCG AGGAGCCATT CCGTATGCCC TGAGCCTTCA CTTGGAGCTG 
GAACCGATTG AGAAACGCCA GCTGATAGGA ACCACCACTA TAATTATCGT GCTCTTCACA 
ATTCTGATTT TGGGAGGGGG CACCATGCCC CTCATTCGCC TGATCGACAT CGAGGACTCC 
AAAGCACGCC GAAGGAGCAA AAAAGATGTG AACCTCAGTA AGACGGAAAA AATGGGTAAC 
ACCATTGAGT CTGAGCATTT GTCAGAGCTG ACAGAAGAGG AATATGAGGC CCAGTACATC 
AGACAACACA ACCTTAAAGG GTTTATGTGG CTGGATGCAA AGTATTTAAA CCCTTTCTTT 
ACTCGGAGGC TGACCCGGGA GGACCTTCAT CACGGGCGCA TACAGATGAA GACCCTGACC 
AATAAGTGGT ATGAAGAGGT GCGCCAAGGG CCTTCAGGGT CCGAGGATGA TGAGCAGGAG 
CTTCTATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P