tbc1d4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	tbc1d4
Entrez Gene ID	100379738
Full Name	TBC1 domain family member 4
General protein information	Preferred Names TBC1 domain family member 4 Names TBC1 domain family member 4
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004911830.3	XP_004911887.1	TBC1 domain family member 4 isoform X1
XM_031897106.1	XP_031752966.1	TBC1 domain family member 4 isoform X1
XM_002931938.4	XP_002931984.2	TBC1 domain family member 4 isoform X2
XM_004911830.4	XP_004911887.2	TBC1 domain family member 4 isoform X1
XM_002931938.5	XP_002931984.3	TBC1 domain family member 4 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	TBC1D4	NP_055647.2
Pan troglodytes (chimpanzee)	TBC1D4	XP_522684.3
	TBC1D4	NP_001165894.1
Canis lupus familiaris (dog)	TBC1D4	XP_005634029.1
Bos taurus (cattle)	TBC1D4	NP_001193239.1
Danio rerio (zebrafish)	si:ch211-222g23.4	XP_005167833.1
Xenopus tropicalis (tropical clawed frog)	tbc1d4	XP_004911886.1
Rattus norvegicus (Norway rat)	Tbc1d4	XP_006252481.1
Gallus gallus (chicken)	TBC1D4	XP_003640601.2
Mus musculus (house mouse)	Tbc1d4	NP_001074747.2

Gene Ontology

The following tbc1d4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tbc1d4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa52598	XM_004911830.4 Latest version!	Xenopus tropicalis TBC1 domain family member 4 (tbc1d4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa52598	XM_031897106.1 Latest version!	Xenopus tropicalis TBC1 domain family member 4 (tbc1d4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa52599	XM_002931938.5 Latest version!	Xenopus tropicalis TBC1 domain family member 4 (tbc1d4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa05928	XM_004911830.3 Latest version!	Xenopus tropicalis TBC1 domain family member 4 (tbc1d4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa05929	XM_002931938.4 Latest version!	Xenopus tropicalis TBC1 domain family member 4 (tbc1d4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tbc1d4 ( XM_031897106.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031752966.1 Xenopus tropicalis TBC1 domain family member 4 (tbc1d4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa52598
Clone ID Related Accession (Same CDS sequence)	XM_031897106.1 , XM_004911830.4
Accession Version	XM_031897106.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	TBC1 domain family member 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGAA GAAGTTCAGC AGACCTGTGC CACCCTCTTT TGGAAGTAAA TACAATGCCA 
GACAAGGTGC TCAAGGAGGA GAAAAGATTT CACTTGTGGT ACATTGGGTG GTCTTCCCTA 
GACCGTCGGA CAACCTTGCC AATGCTTCCA TGGCTTGTAG CTGAGATCCG CAGAAGGGCA 
GAAAGGCAAG ACCCTCCAGG ATGTGCGGCA CCTTGCAGAG AAGTCAATCT TTTGCTAGTT 
ACCCCACACA TCCGCTGTGT GCCAGCGTTG AATGGCGCCA ATGCCAACAG TGCCGTCTTC 
ATCTTTGAGC ACAAAGCACA GCATATATCC CGCTTCATCC ACAATAGCCA AGATCCTTCT 
TATTTTGCCT ATCTGATTAA GACTCAGCCT GACAACCCTG AATCTGAAAT GGCATGCCAT 
GTGTTCAAGG CCAATACCCC AAGACAGGTT TCTGATATCA TCAGCAGCAT ACGTCAAGTC 
TCCAAAGTTG CACTGAAAGA AGATTCCAAG CCTTGCAGAG ATAGTGAAGA TGCCTTTTAT 
AATTCACAGA AGTTTGAAGT GTTATACTGT GGGAAAGTAG TAATTGCAAA TAAAAAAGCA 
CCATCTACCC TTGTTGATGA CTGTATTGAG AAATTCAGCC AACATGAACG CCAGCGTCTA 
AAGTCATTAA ATGAACAGCA GAGCGCAGAA TGCAGCACTG ATTTTTCTTT AGCAGAAATC 
GATGTCCCAG GTTCCCCTTC TGATATGTTT CTGGAGCAGG ACTCTGAAAA TAATTTCATC 
CCTTCTTCTG AGCATCATTC AGGGATGTTT ACAGTTGGCA GTCAAAGCAA TCTGATTGGT 
TCACGAGTAA CATTTCCTGA GAGGATTTTA GAAGACTCTG GCTTTGAGGA TCAGCAAGAA 
TTTAGAACTC GGTGCAGTAG TGTTACAATT CACAAAAGAG CCCAGGATAA TAAAATACAG 
CCAAGAAGAA GGCATGCAAG TGCCCCAAGC CATGTTCAAC CCACTGATTC AGAGAAGAAT 
AGAACAATGC TCTTTCAGGT TGGACGTTTT GAAATCAATC TCATAAGCCC TGATACTAAA 
ACCGTGGAGC TTGAGAAGAA TTTCAAAGAT ATATCATCAT GTTCTCAGGG TATTAAACAC 
ATTGATCATT TTGGGTTGGT CTGCCGTGAA TGTGTGGAGC CTGGGATAAA TCAGTACATT 
TGTTATGTTT TTCAGTGTGC ATCTGAAGCA TTGGTGGAGG AAGTCATGCT TACTTTGAAG 
CAGGCATTCA GCACTGCTGC TGCCTTACAG AGTGCAAAGA CCCAGATCAA GCTATGTGAA 
GCGTGCCCTA TGCATGCCTT GCACAAGCTT TGTGAACGAA TTGAAGGCCT TTACCCACCA 
AGAGCAAAGC TTGTAATACA AAAGCAGCTG TCTTTGCTGA CTGATAACGA ACAAGCAGAT 
ATCTTTGAAC GTGTCCAGAA AATGAAGGCC ACCAGTGACC AAGAAGAAAA TGAGCTTGTG 
ATTCTGCATC TAAGGCAGTT ATGTGAGACA AAACAAAAGA CCCATGTTCA TATAGGAGAA 
GTGCAACAGA ATCTGCCATG CAACACAGTT CCAGAAAATG CAGCTCCAAG TGGCAGATTT 
AAACTGGATA TTCTGAAGAA CAAGGCTAAG AAATCCTTGA CCAGCTCTTT GGAAAATATC 
TTTTCAAGGG GAACAAATAA AATGAGAGGA CGATTGGGCA GTATGGACAG TTTTGAACGT 
TGTAACAGTT TTACATCTGA CAAGGACGGA TCACCCGGGG ACTCACCTCC TGCCACACCA 
CCATCTTCCC CTGTGTCCTC TTCCTGCCCG CCATTTCCAG AGGAAGATCC CGACTCACCT 
AAGGTTCGCA GACGAGCTCA CACTTTCAGC CACCCCACAT CCAGTGGCAA GCGGAGGATA 
ACATTTCCTG ACAGTAAATC ACCGAGTGCC AGGTTCCCCC TCCAGAGACA GAATTCCTTA 
GCAAATGATG TGGGACAAAG CAGCCCAGTT CCTATCGCTC GGCGATTTCG CACTGAGAGT 
GAGTCCTCGT CCTCCAGTCT CCCCTCATCC TTCTCTGCCC CTTCTTTTCT GAAAAATTTT 
TACCAGAATT CAGGGAGACA TGTCCCACAA TCTGAGAATG ACACCCCCAA GAGTGAAAGC 
GAAGGACGAA GGAGAACATT TTCTTCATGT AGCAACGACT CATTAAATGC TAGTGGGTCC 
TGTCTAATAC CACATCGAAT ATCGTGGCGG CAGCGGATTT TCCTCAGGGT AGCATCTCCA 
ATGACCAACT CTCTATCAGA TATGCAGCAT AAAGGCCAGC TCGATGTGAA CGACTTGCTG 
CCATTATCTC CTCTTCCTCC TGCAATGGAA GAAGACCCTC TAGCTGCTCT TCTTCAGGCA 
GAAGACTCGG CACTACAATC AGAAGGGAAG CGAAGCTCTG AAGACTTTCA GAACCTGTGG 
AAGAAAGCAA TACATCAGCA GATATTGTTA CTACGGATGG AAAAGGAGAA TATGAAGCTT 
GAAGCAAGCA GAGATGAACT GCAATCCCGG AAAGTCAAAT TGGACTATGA AGAAGTTGGA 
TTGTGCCAAA AAGAAGTCTT AAATCTTTGG GATAAGAAGT TAAACTGCAG AACAAAGATT 
CGGTTTGATA TGGAAGAGAT TTTTGCCACT CTTAAAGAAG GTGTACCGAA AGGCAAGCGT 
GGAGAGATAT GGCAGTTCCT TTCTGTGCAG TATCGGCTGA GACATCGGCT ACCAAGCAAA 
CAGCAACCAT CTGATGTTTC ATATAATGAT CTTCTCAAGC AACTTACAGC TCAGCAGCAT 
GCTATTCTTG TGGATTTAGG AAGGACCTTC CCCACCCATC CCTACTTCTC TGCCCAGCTG 
GGAGCAGGAC AGTTATCACT GTATAATCTT CTCAAAGCAT ACTCATTATT AGACAAAGAA 
GTTGGTTACT GCCAGGGAAT AAGCTTTGTG GCCGGTGTAC TTCTCCTGCA CATGAGTGAA 
CAGCAAGCTT TTGAAATGCT GAAATTTCTC ATGTATGATC TTGGCTTCCG CAAGCAGTAC 
CGCCCTGATA TGATGTCGCT ACAGATTCAG ATGTACCAGC TTTCCCGGCT TCTCCACGAC 
TACCACCGAG ATCTTTATAA CCACCTGGAG GAGAATGAAA TCAACCCAAG CCTCTATGCA 
GCCCCTTGGT TTCTCACACT TTTCGCTTCA CAGTTTCCCT TAGGTTTTGT TGCTAGGGTT 
TTTGATATCA TTTTTTTACA AGGGACTGAA GTCATTTTCA AAGTGGCTCT TTGCTTGCTC 
AGTAATAGCG AAGCACTTAT TATGGCCCGG GACAGCTTTG AGAGTATTGT GGATTTCCTG 
AAGAGCATGA TACCAGAAAT GACAAATGAA ACCATGGAAA AAATTATTAC ACAGGTTTTT 
GAGATGGACA TTTCAAAGCA ACTCCATGCT TATGAGGTTG AGTACCATGT CATAAATGAT 
GAGATGCTGG AGGCTTCCAT TCCTTCTGAT GACAATGAGT CCTTCCACAA GTTGGAAAAA 
TCCAATAATC AGCTTAAGAG ACAGAACATG GATTTACTGG AAAAACTTCA GGTAGCACAT 
ACGAAGATTC AGAGCTTGGA ATCCAATGTG GAAAATATTT TGTCCAGAGA GAATAAACTG 
AGAAATGTAG TCCGATCGAT GGAAGAGGAA AGATCAGCTT ATCAGAATGT GTTTGAGCTC 
ATGAAAAAAT ATGTGCCGAC CAATGTTCTG ACAGACTGTG AACTCTTGCT GAAAAATACC 
ACATGTAGTC CTAACAACAG GACCAAGGCT GGAAACAAGC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031752966.1
CDS	903..4727
Translation	MERRSSADLCHPLLEVNTMPDKVLKEEKRFHLWYIGWSSLDRRTTLPMLPWLVAEIRRRAERQDPPGCAAPCREVNLLLVTPHIRCVPALNGANANSAVFIFEHKAQHISRFIHNSQDPSYFAYLIKTQPDNPESEMACHVFKANTPRQVSDIISSIRQVSKVALKEDSKPCRDSEDAFYNSQKFEVLYCGKVVIANKKAPSTLVDDCIEKFSQHERQRLKSLNEQQSAECSTDFSLAEIDVPGSPSDMFLEQDSENNFIPSSEHHSGMFTVGSQSNLIGSRVTFPERILEDSGFEDQQEFRTRCSSVTIHKRAQDNKIQPRRRHASAPSHVQPTDSEKNRTMLFQVGRFEINLISPDTKTVELEKNFKDISSCSQGIKHIDHFGLVCRECVEPGINQYICYVFQCASEALVEEVMLTLKQAFSTAAALQSAKTQIKLCEACPMHALHKLCERIEGLYPPRAKLVIQKQLSLLTDNEQADIFERVQKMKATSDQEENELVILHLRQLCETKQKTHVHIGEVQQNLPCNTVPENAAPSGRFKLDILKNKAKKSLTSSLENIFSRGTNKMRGRLGSMDSFERCNSFTSDKDGSPGDSPPATPPSSPVSSSCPPFPEEDPDSPKVRRRAHTFSHPTSSGKRRITFPDSKSPSARFPLQRQNSLANDVGQSSPVPIARRFRTESESSSSSLPSSFSAPSFLKNFYQNSGRHVPQSENDTPKSESEGRRRTFSSCSNDSLNASGSCLIPHRISWRQRIFLRVASPMTNSLSDMQHKGQLDVNDLLPLSPLPPAMEEDPLAALLQAEDSALQSEGKRSSEDFQNLWKKAIHQQILLLRMEKENMKLEASRDELQSRKVKLDYEEVGLCQKEVLNLWDKKLNCRTKIRFDMEEIFATLKEGVPKGKRGEIWQFLSVQYRLRHRLPSKQQPSDVSYNDLLKQLTAQQHAILVDLGRTFPTHPYFSAQLGAGQLSLYNLLKAYSLLDKEVGYCQGISFVAGVLLLHMSEQQAFEMLKFLMYDLGFRKQYRPDMMSLQIQMYQLSRLLHDYHRDLYNHLEENEINPSLYAAPWFLTLFASQFPLGFVARVFDIIFLQGTEVIFKVALCLLSNSEALIMARDSFESIVDFLKSMIPEMTNETMEKIITQVFEMDISKQLHAYEVEYHVINDEMLEASIPSDDNESFHKLEKSNNQLKRQNMDLLEKLQVAHTKIQSLESNVENILSRENKLRNVVRSMEEERSAYQNVFELMKKYVPTNVLTDCELLLKNTTCSPNNRTKAGNKQ

Target ORF information:

RefSeq Version	XM_031897106.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis TBC1 domain family member 4 (tbc1d4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031897106.1

ORF Insert Sequence:

ATGGAGCGAA GAAGTTCAGC AGACCTGTGC CACCCTCTTT TGGAAGTAAA TACAATGCCA 
GACAAGGTGC TCAAGGAGGA GAAAAGATTT CACTTGTGGT ACATTGGGTG GTCTTCCCTA 
GACCGTCGGA CAACCTTGCC AATGCTTCCA TGGCTTGTAG CTGAGATCCG CAGAAGGGCA 
GAAAGGCAAG ACCCTCCAGG ATGTGCGGCA CCTTGCAGAG AAGTCAATCT TTTGCTAGTT 
ACCCCACACA TCCGCTGTGT GCCAGCGTTG AATGGCGCCA ATGCCAACAG TGCCGTCTTC 
ATCTTTGAGC ACAAAGCACA GCATATATCC CGCTTCATCC ACAATAGCCA AGATCCTTCT 
TATTTTGCCT ATCTGATTAA GACTCAGCCT GACAACCCTG AATCTGAAAT GGCATGCCAT 
GTGTTCAAGG CCAATACCCC AAGACAGGTT TCTGATATCA TCAGCAGCAT ACGTCAAGTC 
TCCAAAGTTG CACTGAAAGA AGATTCCAAG CCTTGCAGAG ATAGTGAAGA TGCCTTTTAT 
AATTCACAGA AGTTTGAAGT GTTATACTGT GGGAAAGTAG TAATTGCAAA TAAAAAAGCA 
CCATCTACCC TTGTTGATGA CTGTATTGAG AAATTCAGCC AACATGAACG CCAGCGTCTA 
AAGTCATTAA ATGAACAGCA GAGCGCAGAA TGCAGCACTG ATTTTTCTTT AGCAGAAATC 
GATGTCCCAG GTTCCCCTTC TGATATGTTT CTGGAGCAGG ACTCTGAAAA TAATTTCATC 
CCTTCTTCTG AGCATCATTC AGGGATGTTT ACAGTTGGCA GTCAAAGCAA TCTGATTGGT 
TCACGAGTAA CATTTCCTGA GAGGATTTTA GAAGACTCTG GCTTTGAGGA TCAGCAAGAA 
TTTAGAACTC GGTGCAGTAG TGTTACAATT CACAAAAGAG CCCAGGATAA TAAAATACAG 
CCAAGAAGAA GGCATGCAAG TGCCCCAAGC CATGTTCAAC CCACTGATTC AGAGAAGAAT 
AGAACAATGC TCTTTCAGGT TGGACGTTTT GAAATCAATC TCATAAGCCC TGATACTAAA 
ACCGTGGAGC TTGAGAAGAA TTTCAAAGAT ATATCATCAT GTTCTCAGGG TATTAAACAC 
ATTGATCATT TTGGGTTGGT CTGCCGTGAA TGTGTGGAGC CTGGGATAAA TCAGTACATT 
TGTTATGTTT TTCAGTGTGC ATCTGAAGCA TTGGTGGAGG AAGTCATGCT TACTTTGAAG 
CAGGCATTCA GCACTGCTGC TGCCTTACAG AGTGCAAAGA CCCAGATCAA GCTATGTGAA 
GCGTGCCCTA TGCATGCCTT GCACAAGCTT TGTGAACGAA TTGAAGGCCT TTACCCACCA 
AGAGCAAAGC TTGTAATACA AAAGCAGCTG TCTTTGCTGA CTGATAACGA ACAAGCAGAT 
ATCTTTGAAC GTGTCCAGAA AATGAAGGCC ACCAGTGACC AAGAAGAAAA TGAGCTTGTG 
ATTCTGCATC TAAGGCAGTT ATGTGAGACA AAACAAAAGA CCCATGTTCA TATAGGAGAA 
GTGCAACAGA ATCTGCCATG CAACACAGTT CCAGAAAATG CAGCTCCAAG TGGCAGATTT 
AAACTGGATA TTCTGAAGAA CAAGGCTAAG AAATCCTTGA CCAGCTCTTT GGAAAATATC 
TTTTCAAGGG GAACAAATAA AATGAGAGGA CGATTGGGCA GTATGGACAG TTTTGAACGT 
TGTAACAGTT TTACATCTGA CAAGGACGGA TCACCCGGGG ACTCACCTCC TGCCACACCA 
CCATCTTCCC CTGTGTCCTC TTCCTGCCCG CCATTTCCAG AGGAAGATCC CGACTCACCT 
AAGGTTCGCA GACGAGCTCA CACTTTCAGC CACCCCACAT CCAGTGGCAA GCGGAGGATA 
ACATTTCCTG ACAGTAAATC ACCGAGTGCC AGGTTCCCCC TCCAGAGACA GAATTCCTTA 
GCAAATGATG TGGGACAAAG CAGCCCAGTT CCTATCGCTC GGCGATTTCG CACTGAGAGT 
GAGTCCTCGT CCTCCAGTCT CCCCTCATCC TTCTCTGCCC CTTCTTTTCT GAAAAATTTT 
TACCAGAATT CAGGGAGACA TGTCCCACAA TCTGAGAATG ACACCCCCAA GAGTGAAAGC 
GAAGGACGAA GGAGAACATT TTCTTCATGT AGCAACGACT CATTAAATGC TAGTGGGTCC 
TGTCTAATAC CACATCGAAT ATCGTGGCGG CAGCGGATTT TCCTCAGGGT AGCATCTCCA 
ATGACCAACT CTCTATCAGA TATGCAGCAT AAAGGCCAGC TCGATGTGAA CGACTTGCTG 
CCATTATCTC CTCTTCCTCC TGCAATGGAA GAAGACCCTC TAGCTGCTCT TCTTCAGGCA 
GAAGACTCGG CACTACAATC AGAAGGGAAG CGAAGCTCTG AAGACTTTCA GAACCTGTGG 
AAGAAAGCAA TACATCAGCA GATATTGTTA CTACGGATGG AAAAGGAGAA TATGAAGCTT 
GAAGCAAGCA GAGATGAACT GCAATCCCGG AAAGTCAAAT TGGACTATGA AGAAGTTGGA 
TTGTGCCAAA AAGAAGTCTT AAATCTTTGG GATAAGAAGT TAAACTGCAG AACAAAGATT 
CGGTTTGATA TGGAAGAGAT TTTTGCCACT CTTAAAGAAG GTGTACCGAA AGGCAAGCGT 
GGAGAGATAT GGCAGTTCCT TTCTGTGCAG TATCGGCTGA GACATCGGCT ACCAAGCAAA 
CAGCAACCAT CTGATGTTTC ATATAATGAT CTTCTCAAGC AACTTACAGC TCAGCAGCAT 
GCTATTCTTG TGGATTTAGG AAGGACCTTC CCCACCCATC CCTACTTCTC TGCCCAGCTG 
GGAGCAGGAC AGTTATCACT GTATAATCTT CTCAAAGCAT ACTCATTATT AGACAAAGAA 
GTTGGTTACT GCCAGGGAAT AAGCTTTGTG GCCGGTGTAC TTCTCCTGCA CATGAGTGAA 
CAGCAAGCTT TTGAAATGCT GAAATTTCTC ATGTATGATC TTGGCTTCCG CAAGCAGTAC 
CGCCCTGATA TGATGTCGCT ACAGATTCAG ATGTACCAGC TTTCCCGGCT TCTCCACGAC 
TACCACCGAG ATCTTTATAA CCACCTGGAG GAGAATGAAA TCAACCCAAG CCTCTATGCA 
GCCCCTTGGT TTCTCACACT TTTCGCTTCA CAGTTTCCCT TAGGTTTTGT TGCTAGGGTT 
TTTGATATCA TTTTTTTACA AGGGACTGAA GTCATTTTCA AAGTGGCTCT TTGCTTGCTC 
AGTAATAGCG AAGCACTTAT TATGGCCCGG GACAGCTTTG AGAGTATTGT GGATTTCCTG 
AAGAGCATGA TACCAGAAAT GACAAATGAA ACCATGGAAA AAATTATTAC ACAGGTTTTT 
GAGATGGACA TTTCAAAGCA ACTCCATGCT TATGAGGTTG AGTACCATGT CATAAATGAT 
GAGATGCTGG AGGCTTCCAT TCCTTCTGAT GACAATGAGT CCTTCCACAA GTTGGAAAAA 
TCCAATAATC AGCTTAAGAG ACAGAACATG GATTTACTGG AAAAACTTCA GGTAGCACAT 
ACGAAGATTC AGAGCTTGGA ATCCAATGTG GAAAATATTT TGTCCAGAGA GAATAAACTG 
AGAAATGTAG TCCGATCGAT GGAAGAGGAA AGATCAGCTT ATCAGAATGT GTTTGAGCTC 
ATGAAAAAAT ATGTGCCGAC CAATGTTCTG ACAGACTGTG AACTCTTGCT GAAAAATACC 
ACATGTAGTC CTAACAACAG GACCAAGGCT GGAAACAAGC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tbc1d4 ( XM_004911830.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004911887.2 Xenopus tropicalis TBC1 domain family member 4 (tbc1d4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa52598
Clone ID Related Accession (Same CDS sequence)	XM_031897106.1 , XM_004911830.4
Accession Version	XM_004911830.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	TBC1 domain family member 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004911830.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGAA GAAGTTCAGC AGACCTGTGC CACCCTCTTT TGGAAGTAAA TACAATGCCA 
GACAAGGTGC TCAAGGAGGA GAAAAGATTT CACTTGTGGT ACATTGGGTG GTCTTCCCTA 
GACCGTCGGA CAACCTTGCC AATGCTTCCA TGGCTTGTAG CTGAGATCCG CAGAAGGGCA 
GAAAGGCAAG ACCCTCCAGG ATGTGCGGCA CCTTGCAGAG AAGTCAATCT TTTGCTAGTT 
ACCCCACACA TCCGCTGTGT GCCAGCGTTG AATGGCGCCA ATGCCAACAG TGCCGTCTTC 
ATCTTTGAGC ACAAAGCACA GCATATATCC CGCTTCATCC ACAATAGCCA AGATCCTTCT 
TATTTTGCCT ATCTGATTAA GACTCAGCCT GACAACCCTG AATCTGAAAT GGCATGCCAT 
GTGTTCAAGG CCAATACCCC AAGACAGGTT TCTGATATCA TCAGCAGCAT ACGTCAAGTC 
TCCAAAGTTG CACTGAAAGA AGATTCCAAG CCTTGCAGAG ATAGTGAAGA TGCCTTTTAT 
AATTCACAGA AGTTTGAAGT GTTATACTGT GGGAAAGTAG TAATTGCAAA TAAAAAAGCA 
CCATCTACCC TTGTTGATGA CTGTATTGAG AAATTCAGCC AACATGAACG CCAGCGTCTA 
AAGTCATTAA ATGAACAGCA GAGCGCAGAA TGCAGCACTG ATTTTTCTTT AGCAGAAATC 
GATGTCCCAG GTTCCCCTTC TGATATGTTT CTGGAGCAGG ACTCTGAAAA TAATTTCATC 
CCTTCTTCTG AGCATCATTC AGGGATGTTT ACAGTTGGCA GTCAAAGCAA TCTGATTGGT 
TCACGAGTAA CATTTCCTGA GAGGATTTTA GAAGACTCTG GCTTTGAGGA TCAGCAAGAA 
TTTAGAACTC GGTGCAGTAG TGTTACAATT CACAAAAGAG CCCAGGATAA TAAAATACAG 
CCAAGAAGAA GGCATGCAAG TGCCCCAAGC CATGTTCAAC CCACTGATTC AGAGAAGAAT 
AGAACAATGC TCTTTCAGGT TGGACGTTTT GAAATCAATC TCATAAGCCC TGATACTAAA 
ACCGTGGAGC TTGAGAAGAA TTTCAAAGAT ATATCATCAT GTTCTCAGGG TATTAAACAC 
ATTGATCATT TTGGGTTGGT CTGCCGTGAA TGTGTGGAGC CTGGGATAAA TCAGTACATT 
TGTTATGTTT TTCAGTGTGC ATCTGAAGCA TTGGTGGAGG AAGTCATGCT TACTTTGAAG 
CAGGCATTCA GCACTGCTGC TGCCTTACAG AGTGCAAAGA CCCAGATCAA GCTATGTGAA 
GCGTGCCCTA TGCATGCCTT GCACAAGCTT TGTGAACGAA TTGAAGGCCT TTACCCACCA 
AGAGCAAAGC TTGTAATACA AAAGCAGCTG TCTTTGCTGA CTGATAACGA ACAAGCAGAT 
ATCTTTGAAC GTGTCCAGAA AATGAAGGCC ACCAGTGACC AAGAAGAAAA TGAGCTTGTG 
ATTCTGCATC TAAGGCAGTT ATGTGAGACA AAACAAAAGA CCCATGTTCA TATAGGAGAA 
GTGCAACAGA ATCTGCCATG CAACACAGTT CCAGAAAATG CAGCTCCAAG TGGCAGATTT 
AAACTGGATA TTCTGAAGAA CAAGGCTAAG AAATCCTTGA CCAGCTCTTT GGAAAATATC 
TTTTCAAGGG GAACAAATAA AATGAGAGGA CGATTGGGCA GTATGGACAG TTTTGAACGT 
TGTAACAGTT TTACATCTGA CAAGGACGGA TCACCCGGGG ACTCACCTCC TGCCACACCA 
CCATCTTCCC CTGTGTCCTC TTCCTGCCCG CCATTTCCAG AGGAAGATCC CGACTCACCT 
AAGGTTCGCA GACGAGCTCA CACTTTCAGC CACCCCACAT CCAGTGGCAA GCGGAGGATA 
ACATTTCCTG ACAGTAAATC ACCGAGTGCC AGGTTCCCCC TCCAGAGACA GAATTCCTTA 
GCAAATGATG TGGGACAAAG CAGCCCAGTT CCTATCGCTC GGCGATTTCG CACTGAGAGT 
GAGTCCTCGT CCTCCAGTCT CCCCTCATCC TTCTCTGCCC CTTCTTTTCT GAAAAATTTT 
TACCAGAATT CAGGGAGACA TGTCCCACAA TCTGAGAATG ACACCCCCAA GAGTGAAAGC 
GAAGGACGAA GGAGAACATT TTCTTCATGT AGCAACGACT CATTAAATGC TAGTGGGTCC 
TGTCTAATAC CACATCGAAT ATCGTGGCGG CAGCGGATTT TCCTCAGGGT AGCATCTCCA 
ATGACCAACT CTCTATCAGA TATGCAGCAT AAAGGCCAGC TCGATGTGAA CGACTTGCTG 
CCATTATCTC CTCTTCCTCC TGCAATGGAA GAAGACCCTC TAGCTGCTCT TCTTCAGGCA 
GAAGACTCGG CACTACAATC AGAAGGGAAG CGAAGCTCTG AAGACTTTCA GAACCTGTGG 
AAGAAAGCAA TACATCAGCA GATATTGTTA CTACGGATGG AAAAGGAGAA TATGAAGCTT 
GAAGCAAGCA GAGATGAACT GCAATCCCGG AAAGTCAAAT TGGACTATGA AGAAGTTGGA 
TTGTGCCAAA AAGAAGTCTT AAATCTTTGG GATAAGAAGT TAAACTGCAG AACAAAGATT 
CGGTTTGATA TGGAAGAGAT TTTTGCCACT CTTAAAGAAG GTGTACCGAA AGGCAAGCGT 
GGAGAGATAT GGCAGTTCCT TTCTGTGCAG TATCGGCTGA GACATCGGCT ACCAAGCAAA 
CAGCAACCAT CTGATGTTTC ATATAATGAT CTTCTCAAGC AACTTACAGC TCAGCAGCAT 
GCTATTCTTG TGGATTTAGG AAGGACCTTC CCCACCCATC CCTACTTCTC TGCCCAGCTG 
GGAGCAGGAC AGTTATCACT GTATAATCTT CTCAAAGCAT ACTCATTATT AGACAAAGAA 
GTTGGTTACT GCCAGGGAAT AAGCTTTGTG GCCGGTGTAC TTCTCCTGCA CATGAGTGAA 
CAGCAAGCTT TTGAAATGCT GAAATTTCTC ATGTATGATC TTGGCTTCCG CAAGCAGTAC 
CGCCCTGATA TGATGTCGCT ACAGATTCAG ATGTACCAGC TTTCCCGGCT TCTCCACGAC 
TACCACCGAG ATCTTTATAA CCACCTGGAG GAGAATGAAA TCAACCCAAG CCTCTATGCA 
GCCCCTTGGT TTCTCACACT TTTCGCTTCA CAGTTTCCCT TAGGTTTTGT TGCTAGGGTT 
TTTGATATCA TTTTTTTACA AGGGACTGAA GTCATTTTCA AAGTGGCTCT TTGCTTGCTC 
AGTAATAGCG AAGCACTTAT TATGGCCCGG GACAGCTTTG AGAGTATTGT GGATTTCCTG 
AAGAGCATGA TACCAGAAAT GACAAATGAA ACCATGGAAA AAATTATTAC ACAGGTTTTT 
GAGATGGACA TTTCAAAGCA ACTCCATGCT TATGAGGTTG AGTACCATGT CATAAATGAT 
GAGATGCTGG AGGCTTCCAT TCCTTCTGAT GACAATGAGT CCTTCCACAA GTTGGAAAAA 
TCCAATAATC AGCTTAAGAG ACAGAACATG GATTTACTGG AAAAACTTCA GGTAGCACAT 
ACGAAGATTC AGAGCTTGGA ATCCAATGTG GAAAATATTT TGTCCAGAGA GAATAAACTG 
AGAAATGTAG TCCGATCGAT GGAAGAGGAA AGATCAGCTT ATCAGAATGT GTTTGAGCTC 
ATGAAAAAAT ATGTGCCGAC CAATGTTCTG ACAGACTGTG AACTCTTGCT GAAAAATACC 
ACATGTAGTC CTAACAACAG GACCAAGGCT GGAAACAAGC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004911887.2
CDS	755..4579
Translation	MERRSSADLCHPLLEVNTMPDKVLKEEKRFHLWYIGWSSLDRRTTLPMLPWLVAEIRRRAERQDPPGCAAPCREVNLLLVTPHIRCVPALNGANANSAVFIFEHKAQHISRFIHNSQDPSYFAYLIKTQPDNPESEMACHVFKANTPRQVSDIISSIRQVSKVALKEDSKPCRDSEDAFYNSQKFEVLYCGKVVIANKKAPSTLVDDCIEKFSQHERQRLKSLNEQQSAECSTDFSLAEIDVPGSPSDMFLEQDSENNFIPSSEHHSGMFTVGSQSNLIGSRVTFPERILEDSGFEDQQEFRTRCSSVTIHKRAQDNKIQPRRRHASAPSHVQPTDSEKNRTMLFQVGRFEINLISPDTKTVELEKNFKDISSCSQGIKHIDHFGLVCRECVEPGINQYICYVFQCASEALVEEVMLTLKQAFSTAAALQSAKTQIKLCEACPMHALHKLCERIEGLYPPRAKLVIQKQLSLLTDNEQADIFERVQKMKATSDQEENELVILHLRQLCETKQKTHVHIGEVQQNLPCNTVPENAAPSGRFKLDILKNKAKKSLTSSLENIFSRGTNKMRGRLGSMDSFERCNSFTSDKDGSPGDSPPATPPSSPVSSSCPPFPEEDPDSPKVRRRAHTFSHPTSSGKRRITFPDSKSPSARFPLQRQNSLANDVGQSSPVPIARRFRTESESSSSSLPSSFSAPSFLKNFYQNSGRHVPQSENDTPKSESEGRRRTFSSCSNDSLNASGSCLIPHRISWRQRIFLRVASPMTNSLSDMQHKGQLDVNDLLPLSPLPPAMEEDPLAALLQAEDSALQSEGKRSSEDFQNLWKKAIHQQILLLRMEKENMKLEASRDELQSRKVKLDYEEVGLCQKEVLNLWDKKLNCRTKIRFDMEEIFATLKEGVPKGKRGEIWQFLSVQYRLRHRLPSKQQPSDVSYNDLLKQLTAQQHAILVDLGRTFPTHPYFSAQLGAGQLSLYNLLKAYSLLDKEVGYCQGISFVAGVLLLHMSEQQAFEMLKFLMYDLGFRKQYRPDMMSLQIQMYQLSRLLHDYHRDLYNHLEENEINPSLYAAPWFLTLFASQFPLGFVARVFDIIFLQGTEVIFKVALCLLSNSEALIMARDSFESIVDFLKSMIPEMTNETMEKIITQVFEMDISKQLHAYEVEYHVINDEMLEASIPSDDNESFHKLEKSNNQLKRQNMDLLEKLQVAHTKIQSLESNVENILSRENKLRNVVRSMEEERSAYQNVFELMKKYVPTNVLTDCELLLKNTTCSPNNRTKAGNKQ

Target ORF information:

RefSeq Version	XM_004911830.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis TBC1 domain family member 4 (tbc1d4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911830.4

ORF Insert Sequence:

ATGGAGCGAA GAAGTTCAGC AGACCTGTGC CACCCTCTTT TGGAAGTAAA TACAATGCCA 
GACAAGGTGC TCAAGGAGGA GAAAAGATTT CACTTGTGGT ACATTGGGTG GTCTTCCCTA 
GACCGTCGGA CAACCTTGCC AATGCTTCCA TGGCTTGTAG CTGAGATCCG CAGAAGGGCA 
GAAAGGCAAG ACCCTCCAGG ATGTGCGGCA CCTTGCAGAG AAGTCAATCT TTTGCTAGTT 
ACCCCACACA TCCGCTGTGT GCCAGCGTTG AATGGCGCCA ATGCCAACAG TGCCGTCTTC 
ATCTTTGAGC ACAAAGCACA GCATATATCC CGCTTCATCC ACAATAGCCA AGATCCTTCT 
TATTTTGCCT ATCTGATTAA GACTCAGCCT GACAACCCTG AATCTGAAAT GGCATGCCAT 
GTGTTCAAGG CCAATACCCC AAGACAGGTT TCTGATATCA TCAGCAGCAT ACGTCAAGTC 
TCCAAAGTTG CACTGAAAGA AGATTCCAAG CCTTGCAGAG ATAGTGAAGA TGCCTTTTAT 
AATTCACAGA AGTTTGAAGT GTTATACTGT GGGAAAGTAG TAATTGCAAA TAAAAAAGCA 
CCATCTACCC TTGTTGATGA CTGTATTGAG AAATTCAGCC AACATGAACG CCAGCGTCTA 
AAGTCATTAA ATGAACAGCA GAGCGCAGAA TGCAGCACTG ATTTTTCTTT AGCAGAAATC 
GATGTCCCAG GTTCCCCTTC TGATATGTTT CTGGAGCAGG ACTCTGAAAA TAATTTCATC 
CCTTCTTCTG AGCATCATTC AGGGATGTTT ACAGTTGGCA GTCAAAGCAA TCTGATTGGT 
TCACGAGTAA CATTTCCTGA GAGGATTTTA GAAGACTCTG GCTTTGAGGA TCAGCAAGAA 
TTTAGAACTC GGTGCAGTAG TGTTACAATT CACAAAAGAG CCCAGGATAA TAAAATACAG 
CCAAGAAGAA GGCATGCAAG TGCCCCAAGC CATGTTCAAC CCACTGATTC AGAGAAGAAT 
AGAACAATGC TCTTTCAGGT TGGACGTTTT GAAATCAATC TCATAAGCCC TGATACTAAA 
ACCGTGGAGC TTGAGAAGAA TTTCAAAGAT ATATCATCAT GTTCTCAGGG TATTAAACAC 
ATTGATCATT TTGGGTTGGT CTGCCGTGAA TGTGTGGAGC CTGGGATAAA TCAGTACATT 
TGTTATGTTT TTCAGTGTGC ATCTGAAGCA TTGGTGGAGG AAGTCATGCT TACTTTGAAG 
CAGGCATTCA GCACTGCTGC TGCCTTACAG AGTGCAAAGA CCCAGATCAA GCTATGTGAA 
GCGTGCCCTA TGCATGCCTT GCACAAGCTT TGTGAACGAA TTGAAGGCCT TTACCCACCA 
AGAGCAAAGC TTGTAATACA AAAGCAGCTG TCTTTGCTGA CTGATAACGA ACAAGCAGAT 
ATCTTTGAAC GTGTCCAGAA AATGAAGGCC ACCAGTGACC AAGAAGAAAA TGAGCTTGTG 
ATTCTGCATC TAAGGCAGTT ATGTGAGACA AAACAAAAGA CCCATGTTCA TATAGGAGAA 
GTGCAACAGA ATCTGCCATG CAACACAGTT CCAGAAAATG CAGCTCCAAG TGGCAGATTT 
AAACTGGATA TTCTGAAGAA CAAGGCTAAG AAATCCTTGA CCAGCTCTTT GGAAAATATC 
TTTTCAAGGG GAACAAATAA AATGAGAGGA CGATTGGGCA GTATGGACAG TTTTGAACGT 
TGTAACAGTT TTACATCTGA CAAGGACGGA TCACCCGGGG ACTCACCTCC TGCCACACCA 
CCATCTTCCC CTGTGTCCTC TTCCTGCCCG CCATTTCCAG AGGAAGATCC CGACTCACCT 
AAGGTTCGCA GACGAGCTCA CACTTTCAGC CACCCCACAT CCAGTGGCAA GCGGAGGATA 
ACATTTCCTG ACAGTAAATC ACCGAGTGCC AGGTTCCCCC TCCAGAGACA GAATTCCTTA 
GCAAATGATG TGGGACAAAG CAGCCCAGTT CCTATCGCTC GGCGATTTCG CACTGAGAGT 
GAGTCCTCGT CCTCCAGTCT CCCCTCATCC TTCTCTGCCC CTTCTTTTCT GAAAAATTTT 
TACCAGAATT CAGGGAGACA TGTCCCACAA TCTGAGAATG ACACCCCCAA GAGTGAAAGC 
GAAGGACGAA GGAGAACATT TTCTTCATGT AGCAACGACT CATTAAATGC TAGTGGGTCC 
TGTCTAATAC CACATCGAAT ATCGTGGCGG CAGCGGATTT TCCTCAGGGT AGCATCTCCA 
ATGACCAACT CTCTATCAGA TATGCAGCAT AAAGGCCAGC TCGATGTGAA CGACTTGCTG 
CCATTATCTC CTCTTCCTCC TGCAATGGAA GAAGACCCTC TAGCTGCTCT TCTTCAGGCA 
GAAGACTCGG CACTACAATC AGAAGGGAAG CGAAGCTCTG AAGACTTTCA GAACCTGTGG 
AAGAAAGCAA TACATCAGCA GATATTGTTA CTACGGATGG AAAAGGAGAA TATGAAGCTT 
GAAGCAAGCA GAGATGAACT GCAATCCCGG AAAGTCAAAT TGGACTATGA AGAAGTTGGA 
TTGTGCCAAA AAGAAGTCTT AAATCTTTGG GATAAGAAGT TAAACTGCAG AACAAAGATT 
CGGTTTGATA TGGAAGAGAT TTTTGCCACT CTTAAAGAAG GTGTACCGAA AGGCAAGCGT 
GGAGAGATAT GGCAGTTCCT TTCTGTGCAG TATCGGCTGA GACATCGGCT ACCAAGCAAA 
CAGCAACCAT CTGATGTTTC ATATAATGAT CTTCTCAAGC AACTTACAGC TCAGCAGCAT 
GCTATTCTTG TGGATTTAGG AAGGACCTTC CCCACCCATC CCTACTTCTC TGCCCAGCTG 
GGAGCAGGAC AGTTATCACT GTATAATCTT CTCAAAGCAT ACTCATTATT AGACAAAGAA 
GTTGGTTACT GCCAGGGAAT AAGCTTTGTG GCCGGTGTAC TTCTCCTGCA CATGAGTGAA 
CAGCAAGCTT TTGAAATGCT GAAATTTCTC ATGTATGATC TTGGCTTCCG CAAGCAGTAC 
CGCCCTGATA TGATGTCGCT ACAGATTCAG ATGTACCAGC TTTCCCGGCT TCTCCACGAC 
TACCACCGAG ATCTTTATAA CCACCTGGAG GAGAATGAAA TCAACCCAAG CCTCTATGCA 
GCCCCTTGGT TTCTCACACT TTTCGCTTCA CAGTTTCCCT TAGGTTTTGT TGCTAGGGTT 
TTTGATATCA TTTTTTTACA AGGGACTGAA GTCATTTTCA AAGTGGCTCT TTGCTTGCTC 
AGTAATAGCG AAGCACTTAT TATGGCCCGG GACAGCTTTG AGAGTATTGT GGATTTCCTG 
AAGAGCATGA TACCAGAAAT GACAAATGAA ACCATGGAAA AAATTATTAC ACAGGTTTTT 
GAGATGGACA TTTCAAAGCA ACTCCATGCT TATGAGGTTG AGTACCATGT CATAAATGAT 
GAGATGCTGG AGGCTTCCAT TCCTTCTGAT GACAATGAGT CCTTCCACAA GTTGGAAAAA 
TCCAATAATC AGCTTAAGAG ACAGAACATG GATTTACTGG AAAAACTTCA GGTAGCACAT 
ACGAAGATTC AGAGCTTGGA ATCCAATGTG GAAAATATTT TGTCCAGAGA GAATAAACTG 
AGAAATGTAG TCCGATCGAT GGAAGAGGAA AGATCAGCTT ATCAGAATGT GTTTGAGCTC 
ATGAAAAAAT ATGTGCCGAC CAATGTTCTG ACAGACTGTG AACTCTTGCT GAAAAATACC 
ACATGTAGTC CTAACAACAG GACCAAGGCT GGAAACAAGC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tbc1d4 ( XM_002931938.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002931984.3 Xenopus tropicalis TBC1 domain family member 4 (tbc1d4), transcript variant X3, mRNA.

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CloneID	OXa52599
Clone ID Related Accession (Same CDS sequence)	XM_002931938.5
Accession Version	XM_002931938.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	TBC1 domain family member 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002931938.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGAA GAAGTTCAGC AGACCTGTGC CACCCTCTTT TGGAAGTAAA TACAATGCCA 
GACAAGGTGC TCAAGGAGGA GAAAAGATTT CACTTGTGGT ACATTGGGTG GTCTTCCCTA 
GACCGTCGGA CAACCTTGCC AATGCTTCCA TGGCTTGTAG CTGAGATCCG CAGAAGGGCA 
GAAAGGCAAG ACCCTCCAGG ATGTGCGGCA CCTTGCAGAG AAGTCAATCT TTTGCTAGTT 
ACCCCACACA TCCGCTGTGT GCCAGCGTTG AATGGCGCCA ATGCCAACAG TGCCGTCTTC 
ATCTTTGAGC ACAAAGCACA GCATATATCC CGCTTCATCC ACAATAGCCA AGATCCTTCT 
TATTTTGCCT ATCTGATTAA GACTCAGCCT GACAACCCTG AATCTGAAAT GGCATGCCAT 
GTGTTCAAGG CCAATACCCC AAGACAGGTT TCTGATATCA TCAGCAGCAT ACGTCAAGTC 
TCCAAAGTTG CACTGAAAGA AGATTCCAAG CCTTGCAGAG ATAGTGAAGA TGCCTTTTAT 
AATTCACAGA AGTTTGAAGT GTTATACTGT GGGAAAGTAG TAATTGCAAA TAAAAAAGCA 
CCATCTACCC TTGTTGATGA CTGTATTGAG AAATTCAGCC AACATGAACG CCAGCGTCTA 
AAGTCATTAA ATGAACAGCA GAGCGCAGAA TGCAGCACTG ATTTTTCTTT AGCAGAAATC 
GATGTCCCAG GTTCCCCTTC TGATATGTTT CTGGAGCAGG ACTCTGAAAA TAATTTCATC 
CCTTCTTCTG AGCATCATTC AGGGATGTTT ACAGTTGGCA GTCAAAGCAA TCTGATTGGT 
TCACGAGTAA CATTTCCTGA GAGGATTTTA GAAGACTCTG GCTTTGAGGA TCAGCAAGAA 
TTTAGAACTC GGTGCAGTAG TGTTACAATT CACAAAAGAG CCCAGGATAA TAAAATACAG 
CCAAGAAGAA GGCATGCAAG TGCCCCAAGC CATGTTCAAC CCACTGATTC AGAGAAGAAT 
AGAACAATGC TCTTTCAGGT TGGACGTTTT GAAATCAATC TCATAAGCCC TGATACTAAA 
ACCGTGGAGC TTGAGAAGAA TTTCAAAGAT ATATCATCAT GTTCTCAGGG TATTAAACAC 
ATTGATCATT TTGGGTTGGT CTGCCGTGAA TGTGTGGAGC CTGGGATAAA TCAGTACATT 
TGTTATGTTT TTCAGTGTGC ATCTGAAGCA TTGGTGGAGG AAGTCATGCT TACTTTGAAG 
CAGGCATTCA GCACTGCTGC TGCCTTACAG AGTGCAAAGA CCCAGATCAA GCTATGTGAA 
GCGTGCCCTA TGCATGCCTT GCACAAGCTT TGTGAACGAA TTGAAGGCCT TTACCCACCA 
AGAGCAAAGC TTGTAATACA AAAGCAGCTG TCTTTGCTGA CTGATAACGA ACAAGCAGAT 
ATCTTTGAAC GTGTCCAGAA AATGAAGGCC ACCAGTGACC AAGAAGAAAA TGAGCTTGTG 
ATTCTGCATC TAAGGCAGTT ATGTGAGACA AAACAAAAGA CCCATGTTCA TATAGGAGAA 
GTGCAACAGA ATCTGCCATG CAACACAGTT CCAGAAAATG CAGCTCCAAG TGGCAGATTT 
AAACTGGATA TTCTGAAGAA CAAGGCTAAG AAATCCTTGA CCAGCTCTTT GGAAAATATC 
TTTTCAAGGG GAACAAATAA AATGAGAGGA CGATTGGGCA GTATGGACAG TTTTGAACGT 
TGTAACAGTT TTACATCTGA CAAGGACGGA TCACCCGGGG ACTCACCTCC TGCCACACCA 
CCATCTTCCC CTGTGTCCTC TTCCTGCCCG CCATTTCCAG AGGAAGATCC CGACTCACCT 
AAGGTTCGCA GACGAGCTCA CACTTTCAGC CACCCCACAT CCAGTGGCAA GCGGAGGATA 
ACATTTCCTG ACAGTAAATC ACCGAGTGCC AGGTTCCCCC TCCAGAGACA GAATTCCTTA 
GCAAATGATG TGGGACAAAG CAGTGAAAGC GAAGGACGAA GGAGAACATT TTCTTCATGT 
AGCAACGACT CATTAAATGC TAGTGGGTCC TGTCTAATAC CACATCGAAT ATCGTGGCGG 
CAGCGGATTT TCCTCAGGGT AGCATCTCCA ATGACCAACT CTCTATCAGA TATGCAGCAT 
AAAGGCCAGC TCGATGTGAA CGACTTGCTG CCATTATCTC CTCTTCCTCC TGCAATGGAA 
GAAGACCCTC TAGCTGCTCT TCTTCAGGCA GAAGACTCGG CACTACAATC AGAAGGGAAG 
CGAAGCTCTG AAGACTTTCA GAACCTGTGG AAGAAAGCAA TACATCAGCA GATATTGTTA 
CTACGGATGG AAAAGGAGAA TATGAAGCTT GAAGCAAGCA GAGATGAACT GCAATCCCGG 
AAAGTCAAAT TGGACTATGA AGAAGTTGGA TTGTGCCAAA AAGAAGTCTT AAATCTTTGG 
GATAAGAAGT TAAACTGCAG AACAAAGATT CGGTTTGATA TGGAAGAGAT TTTTGCCACT 
CTTAAAGAAG GTGTACCGAA AGGCAAGCGT GGAGAGATAT GGCAGTTCCT TTCTGTGCAG 
TATCGGCTGA GACATCGGCT ACCAAGCAAA CAGCAACCAT CTGATGTTTC ATATAATGAT 
CTTCTCAAGC AACTTACAGC TCAGCAGCAT GCTATTCTTG TGGATTTAGG AAGGACCTTC 
CCCACCCATC CCTACTTCTC TGCCCAGCTG GGAGCAGGAC AGTTATCACT GTATAATCTT 
CTCAAAGCAT ACTCATTATT AGACAAAGAA GTTGGTTACT GCCAGGGAAT AAGCTTTGTG 
GCCGGTGTAC TTCTCCTGCA CATGAGTGAA CAGCAAGCTT TTGAAATGCT GAAATTTCTC 
ATGTATGATC TTGGCTTCCG CAAGCAGTAC CGCCCTGATA TGATGTCGCT ACAGATTCAG 
ATGTACCAGC TTTCCCGGCT TCTCCACGAC TACCACCGAG ATCTTTATAA CCACCTGGAG 
GAGAATGAAA TCAACCCAAG CCTCTATGCA GCCCCTTGGT TTCTCACACT TTTCGCTTCA 
CAGTTTCCCT TAGGTTTTGT TGCTAGGGTT TTTGATATCA TTTTTTTACA AGGGACTGAA 
GTCATTTTCA AAGTGGCTCT TTGCTTGCTC AGTAATAGCG AAGCACTTAT TATGGCCCGG 
GACAGCTTTG AGAGTATTGT GGATTTCCTG AAGAGCATGA TACCAGAAAT GACAAATGAA 
ACCATGGAAA AAATTATTAC ACAGGTTTTT GAGATGGACA TTTCAAAGCA ACTCCATGCT 
TATGAGGTTG AGTACCATGT CATAAATGAT GAGATGCTGG AGGCTTCCAT TCCTTCTGAT 
GACAATGAGT CCTTCCACAA GTTGGAAAAA TCCAATAATC AGCTTAAGAG ACAGAACATG 
GATTTACTGG AAAAACTTCA GGTAGCACAT ACGAAGATTC AGAGCTTGGA ATCCAATGTG 
GAAAATATTT TGTCCAGAGA GAATAAACTG AGAAATGTAG TCCGATCGAT GGAAGAGGAA 
AGATCAGCTT ATCAGAATGT GTTTGAGCTC ATGAAAAAAT ATGTGCCGAC CAATGTTCTG 
ACAGACTGTG AACTCTTGCT GAAAAATACC ACATGTAGTC CTAACAACAG GACCAAGGCT 
GGAAACAAGC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002931984.3
CDS	903..4577
Translation	MERRSSADLCHPLLEVNTMPDKVLKEEKRFHLWYIGWSSLDRRTTLPMLPWLVAEIRRRAERQDPPGCAAPCREVNLLLVTPHIRCVPALNGANANSAVFIFEHKAQHISRFIHNSQDPSYFAYLIKTQPDNPESEMACHVFKANTPRQVSDIISSIRQVSKVALKEDSKPCRDSEDAFYNSQKFEVLYCGKVVIANKKAPSTLVDDCIEKFSQHERQRLKSLNEQQSAECSTDFSLAEIDVPGSPSDMFLEQDSENNFIPSSEHHSGMFTVGSQSNLIGSRVTFPERILEDSGFEDQQEFRTRCSSVTIHKRAQDNKIQPRRRHASAPSHVQPTDSEKNRTMLFQVGRFEINLISPDTKTVELEKNFKDISSCSQGIKHIDHFGLVCRECVEPGINQYICYVFQCASEALVEEVMLTLKQAFSTAAALQSAKTQIKLCEACPMHALHKLCERIEGLYPPRAKLVIQKQLSLLTDNEQADIFERVQKMKATSDQEENELVILHLRQLCETKQKTHVHIGEVQQNLPCNTVPENAAPSGRFKLDILKNKAKKSLTSSLENIFSRGTNKMRGRLGSMDSFERCNSFTSDKDGSPGDSPPATPPSSPVSSSCPPFPEEDPDSPKVRRRAHTFSHPTSSGKRRITFPDSKSPSARFPLQRQNSLANDVGQSSESEGRRRTFSSCSNDSLNASGSCLIPHRISWRQRIFLRVASPMTNSLSDMQHKGQLDVNDLLPLSPLPPAMEEDPLAALLQAEDSALQSEGKRSSEDFQNLWKKAIHQQILLLRMEKENMKLEASRDELQSRKVKLDYEEVGLCQKEVLNLWDKKLNCRTKIRFDMEEIFATLKEGVPKGKRGEIWQFLSVQYRLRHRLPSKQQPSDVSYNDLLKQLTAQQHAILVDLGRTFPTHPYFSAQLGAGQLSLYNLLKAYSLLDKEVGYCQGISFVAGVLLLHMSEQQAFEMLKFLMYDLGFRKQYRPDMMSLQIQMYQLSRLLHDYHRDLYNHLEENEINPSLYAAPWFLTLFASQFPLGFVARVFDIIFLQGTEVIFKVALCLLSNSEALIMARDSFESIVDFLKSMIPEMTNETMEKIITQVFEMDISKQLHAYEVEYHVINDEMLEASIPSDDNESFHKLEKSNNQLKRQNMDLLEKLQVAHTKIQSLESNVENILSRENKLRNVVRSMEEERSAYQNVFELMKKYVPTNVLTDCELLLKNTTCSPNNRTKAGNKQ

Target ORF information:

RefSeq Version	XM_002931938.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis TBC1 domain family member 4 (tbc1d4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002931938.5

ORF Insert Sequence:

ATGGAGCGAA GAAGTTCAGC AGACCTGTGC CACCCTCTTT TGGAAGTAAA TACAATGCCA 
GACAAGGTGC TCAAGGAGGA GAAAAGATTT CACTTGTGGT ACATTGGGTG GTCTTCCCTA 
GACCGTCGGA CAACCTTGCC AATGCTTCCA TGGCTTGTAG CTGAGATCCG CAGAAGGGCA 
GAAAGGCAAG ACCCTCCAGG ATGTGCGGCA CCTTGCAGAG AAGTCAATCT TTTGCTAGTT 
ACCCCACACA TCCGCTGTGT GCCAGCGTTG AATGGCGCCA ATGCCAACAG TGCCGTCTTC 
ATCTTTGAGC ACAAAGCACA GCATATATCC CGCTTCATCC ACAATAGCCA AGATCCTTCT 
TATTTTGCCT ATCTGATTAA GACTCAGCCT GACAACCCTG AATCTGAAAT GGCATGCCAT 
GTGTTCAAGG CCAATACCCC AAGACAGGTT TCTGATATCA TCAGCAGCAT ACGTCAAGTC 
TCCAAAGTTG CACTGAAAGA AGATTCCAAG CCTTGCAGAG ATAGTGAAGA TGCCTTTTAT 
AATTCACAGA AGTTTGAAGT GTTATACTGT GGGAAAGTAG TAATTGCAAA TAAAAAAGCA 
CCATCTACCC TTGTTGATGA CTGTATTGAG AAATTCAGCC AACATGAACG CCAGCGTCTA 
AAGTCATTAA ATGAACAGCA GAGCGCAGAA TGCAGCACTG ATTTTTCTTT AGCAGAAATC 
GATGTCCCAG GTTCCCCTTC TGATATGTTT CTGGAGCAGG ACTCTGAAAA TAATTTCATC 
CCTTCTTCTG AGCATCATTC AGGGATGTTT ACAGTTGGCA GTCAAAGCAA TCTGATTGGT 
TCACGAGTAA CATTTCCTGA GAGGATTTTA GAAGACTCTG GCTTTGAGGA TCAGCAAGAA 
TTTAGAACTC GGTGCAGTAG TGTTACAATT CACAAAAGAG CCCAGGATAA TAAAATACAG 
CCAAGAAGAA GGCATGCAAG TGCCCCAAGC CATGTTCAAC CCACTGATTC AGAGAAGAAT 
AGAACAATGC TCTTTCAGGT TGGACGTTTT GAAATCAATC TCATAAGCCC TGATACTAAA 
ACCGTGGAGC TTGAGAAGAA TTTCAAAGAT ATATCATCAT GTTCTCAGGG TATTAAACAC 
ATTGATCATT TTGGGTTGGT CTGCCGTGAA TGTGTGGAGC CTGGGATAAA TCAGTACATT 
TGTTATGTTT TTCAGTGTGC ATCTGAAGCA TTGGTGGAGG AAGTCATGCT TACTTTGAAG 
CAGGCATTCA GCACTGCTGC TGCCTTACAG AGTGCAAAGA CCCAGATCAA GCTATGTGAA 
GCGTGCCCTA TGCATGCCTT GCACAAGCTT TGTGAACGAA TTGAAGGCCT TTACCCACCA 
AGAGCAAAGC TTGTAATACA AAAGCAGCTG TCTTTGCTGA CTGATAACGA ACAAGCAGAT 
ATCTTTGAAC GTGTCCAGAA AATGAAGGCC ACCAGTGACC AAGAAGAAAA TGAGCTTGTG 
ATTCTGCATC TAAGGCAGTT ATGTGAGACA AAACAAAAGA CCCATGTTCA TATAGGAGAA 
GTGCAACAGA ATCTGCCATG CAACACAGTT CCAGAAAATG CAGCTCCAAG TGGCAGATTT 
AAACTGGATA TTCTGAAGAA CAAGGCTAAG AAATCCTTGA CCAGCTCTTT GGAAAATATC 
TTTTCAAGGG GAACAAATAA AATGAGAGGA CGATTGGGCA GTATGGACAG TTTTGAACGT 
TGTAACAGTT TTACATCTGA CAAGGACGGA TCACCCGGGG ACTCACCTCC TGCCACACCA 
CCATCTTCCC CTGTGTCCTC TTCCTGCCCG CCATTTCCAG AGGAAGATCC CGACTCACCT 
AAGGTTCGCA GACGAGCTCA CACTTTCAGC CACCCCACAT CCAGTGGCAA GCGGAGGATA 
ACATTTCCTG ACAGTAAATC ACCGAGTGCC AGGTTCCCCC TCCAGAGACA GAATTCCTTA 
GCAAATGATG TGGGACAAAG CAGTGAAAGC GAAGGACGAA GGAGAACATT TTCTTCATGT 
AGCAACGACT CATTAAATGC TAGTGGGTCC TGTCTAATAC CACATCGAAT ATCGTGGCGG 
CAGCGGATTT TCCTCAGGGT AGCATCTCCA ATGACCAACT CTCTATCAGA TATGCAGCAT 
AAAGGCCAGC TCGATGTGAA CGACTTGCTG CCATTATCTC CTCTTCCTCC TGCAATGGAA 
GAAGACCCTC TAGCTGCTCT TCTTCAGGCA GAAGACTCGG CACTACAATC AGAAGGGAAG 
CGAAGCTCTG AAGACTTTCA GAACCTGTGG AAGAAAGCAA TACATCAGCA GATATTGTTA 
CTACGGATGG AAAAGGAGAA TATGAAGCTT GAAGCAAGCA GAGATGAACT GCAATCCCGG 
AAAGTCAAAT TGGACTATGA AGAAGTTGGA TTGTGCCAAA AAGAAGTCTT AAATCTTTGG 
GATAAGAAGT TAAACTGCAG AACAAAGATT CGGTTTGATA TGGAAGAGAT TTTTGCCACT 
CTTAAAGAAG GTGTACCGAA AGGCAAGCGT GGAGAGATAT GGCAGTTCCT TTCTGTGCAG 
TATCGGCTGA GACATCGGCT ACCAAGCAAA CAGCAACCAT CTGATGTTTC ATATAATGAT 
CTTCTCAAGC AACTTACAGC TCAGCAGCAT GCTATTCTTG TGGATTTAGG AAGGACCTTC 
CCCACCCATC CCTACTTCTC TGCCCAGCTG GGAGCAGGAC AGTTATCACT GTATAATCTT 
CTCAAAGCAT ACTCATTATT AGACAAAGAA GTTGGTTACT GCCAGGGAAT AAGCTTTGTG 
GCCGGTGTAC TTCTCCTGCA CATGAGTGAA CAGCAAGCTT TTGAAATGCT GAAATTTCTC 
ATGTATGATC TTGGCTTCCG CAAGCAGTAC CGCCCTGATA TGATGTCGCT ACAGATTCAG 
ATGTACCAGC TTTCCCGGCT TCTCCACGAC TACCACCGAG ATCTTTATAA CCACCTGGAG 
GAGAATGAAA TCAACCCAAG CCTCTATGCA GCCCCTTGGT TTCTCACACT TTTCGCTTCA 
CAGTTTCCCT TAGGTTTTGT TGCTAGGGTT TTTGATATCA TTTTTTTACA AGGGACTGAA 
GTCATTTTCA AAGTGGCTCT TTGCTTGCTC AGTAATAGCG AAGCACTTAT TATGGCCCGG 
GACAGCTTTG AGAGTATTGT GGATTTCCTG AAGAGCATGA TACCAGAAAT GACAAATGAA 
ACCATGGAAA AAATTATTAC ACAGGTTTTT GAGATGGACA TTTCAAAGCA ACTCCATGCT 
TATGAGGTTG AGTACCATGT CATAAATGAT GAGATGCTGG AGGCTTCCAT TCCTTCTGAT 
GACAATGAGT CCTTCCACAA GTTGGAAAAA TCCAATAATC AGCTTAAGAG ACAGAACATG 
GATTTACTGG AAAAACTTCA GGTAGCACAT ACGAAGATTC AGAGCTTGGA ATCCAATGTG 
GAAAATATTT TGTCCAGAGA GAATAAACTG AGAAATGTAG TCCGATCGAT GGAAGAGGAA 
AGATCAGCTT ATCAGAATGT GTTTGAGCTC ATGAAAAAAT ATGTGCCGAC CAATGTTCTG 
ACAGACTGTG AACTCTTGCT GAAAAATACC ACATGTAGTC CTAACAACAG GACCAAGGCT 
GGAAACAAGC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tbc1d4 ( XM_004911830.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004911887.1 Xenopus tropicalis TBC1 domain family member 4 (tbc1d4), transcript variant X1, mRNA.

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CloneID	OXa05928
Clone ID Related Accession (Same CDS sequence)	XM_004911830.3
Accession Version	XM_004911830.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3822bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	TBC1 domain family member 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004911830.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGAA GAAGTTCAGC AGACCTGTGC CACCCTCTTT TGGAAGTAAA TACAATGCCA 
GACAAGGTGC TCAAGGAGGA GAAAAGATTT CACTTGTGGT ACATTGGGTG GTCTTCCCTA 
GACCGTCGGA CAACCTTGCC AATGCTTCCA TGGCTTGTAG CTGAGATCCG CAGAAGGGCA 
GAAAGGCAAG ACCCTCCAGG ATGTGCGGCA CCTTGCAGAG AAGTCAATCT TTTGCTAGTT 
ACCCCACACA TCCGCTGTGT GCCAGCGTTG AATGGCGCCA ATGCCAACAG TGCCGTCTTC 
ATCTTTGAGC ACAAAGCACA GCATATATCC CGCTTCATCC ACAATAGCCA AGATCCTTCT 
TATTTTGCCT ATCTGATTAA GACTCAGCCT GACAACCCTG AATCTGAAAT GGCATGCCAT 
GTGTTCAAGG CCAATACCCC AAGACAGGTT TCTGATATCA TCAGTAGCAT ACGTCAAGTC 
TCCAAAGTTG CACTGAAAGA AGATTCCAAG CCTTGCAGAG ATAGTGAAGA TGCCTTTTAT 
AATTCACAGA AGTTTGAAGT GTTATACTGT GGGAAAGTAG TAATTGCAAA TAAAAAAGCA 
CCATCTACCC TTGTTGATGA CTGTATTGAG AAATTCAGCC AACATGAACG CCAGCGTCTA 
AAGTCATTAA ATGAACAGCA GAGCGCAGAA TGCAGCACTG ATTTTTCTTT AGCAGAAATC 
GATGTCCCAG GTTCCCCTTC TGATATGTTT CTGGAGCAGG ACTCTGAAAA TAATTTCATC 
CCTTCTTCTG AGCATCATTC AGGGATGTTT ACAGTTGGCA GTCAAAGCAA TCTGATTGGT 
TCACGAGTAA CATTTCCTGA GAGGATTTTA GAAGACTCTG GCTTTGAGGA TCAGCAAGAA 
TTTAGAACTC GGTGCAGTAG TGTTACAATT CACAAAAGAG CCCAGGATAA TAAAATACAG 
CCAAGAAGAA GGCATGCAAG TGCCCCAAGC CATGTTCAAC CCACTGATTC AGAGAAGAAT 
AGAACAATGC TCTTTCAGGT TGGACGTTTT GAAATCAATC TCATAAGCCC TGATACTAAA 
ACCGTGGAGC TTGAGAAGAA TTTCAAAGAT ATATCATCAT GTTCTCAGGG TATTAAACAC 
ATTGATCATT TTGGGTTGGT CTGCCGTGAA TGTGTGGAGC CTGGGATAAA TCAGTACATT 
TGTTATGTTT TTCAGTGTGC ATCTGAAGCA TTGGTGGAGG AAGTCATGCT TACTTTGAAG 
CAGGCATTCA GCACTGCTGC TGCCTTACAG AGTGCAAAGA CCCAGATCAA GCTATGTGAA 
GCGTGCCCTA TGCATGCCTT GCACAAGCTT TGTGAACGAA TTGAAGGCCT TTACCCACCA 
AGAGCAAAGC TTGTAATACA AAAGCAGCTG TCTTTGCTGA CTGATAACGA ACAAGCAGAT 
ATCTTTGAAC GTGTCCAGAA AATGAAGGCC ACCAGTGACC AAGAAGAAAA TGAGCTTGTG 
ATTCTGCATC TAAGGCAGTT ATGTGAGACA AAACAAAAGA CCCATGTTCA TATAGGAGAA 
GTGCAACAGA ATCTGCCATG CAACACAGTT CCAGAAAATG CAGCTCCAAG TGGCAGATTT 
AAACTGGATA TTCTGAAGAA CAAGGCTAAG AAATCCTTGA CCAGCTCTTT GGAAAATATC 
TTTTCAAGGG GAACAAATAA AATGAGAGGA CGATTGGGCA GTATGGACAG TTTTGAACGT 
TGTAACAGTT TTACATCTGA CAAGGACGGA TCACCCGGGG ACTCACCTCC TGCCACACCA 
CCATCTTCCC CTGTGTCCTC TTCCTGCCCG CCATTTCCAG AGGAAGATCC CGACTCACCT 
AAGGTTCGCA GACGAGCTCA CACTTTCAGC CACCCCACAT CCAGTGGCAA GCGGAGGATA 
ACATTTCCTG ACAGTAAATC ACCGAGTGCC AGGTTCCCCC TCCAGAGACA GAATTCCTTA 
GCAAATGATG TGGGACAAAG CAGCCCAGTT CCTATCGCTC GGCGATTTCG CACTGAGAGT 
GAGTCCTCGT CCTCCAGTCT CCCCTCATCC TTCTCTGCCC CTTCTTTTCT GAAAAATTTT 
TACCAGAATT CAGGGAGACA TGTCCCACAA TCTGAGAATG ACACCCCCAA GAGTGAAAGC 
GAAGGACGAA GGAGAACATT TTCTTCATGT AGCAACGACT CATTAAATGC TAGTGGGTCC 
TGTCTAATAC CACATCGAAT ATCGTGGCGG CAGCGGATTT TCCTCAGGGT AGCATCTCCA 
ATGACCAACT CTCTATCAGA TATGCAGCAT AAAGGCCAGC TCGATGTGAA CGACTTGCTG 
CCATTATCTC CTCTTCCTCC TGCAATGGAA GAAGACCCTC TAGCTGCTCT TCAGGCAGAA 
GACTCGGCAC TACAATCAGA AGGGAAGCGA AGCTCTGAAG ACTTTCAGAA CCTGTGGAAG 
AAAGCAATAC ATCAGCAGAT ATTGTTACTA CGGATGGAAA AGGAGAATAT GAAGCTTGAA 
GCAAGCAGAG ATGAACTGCA ATCCCGGAAA GTCAAATTGG ACTATGAAGA AGTTGGATTG 
TGCCAAAAAG AAGTCTTAAA TCTTTGGGAT AAGAAGTTAA ACTGCAGAAC AAAGATTCGG 
TTTGATATGG AAGAGATTTT TGCCACTCTT AAAGAAGGTG TACCGAAAGG CAAGCGTGGA 
GAGATATGGC AGTTCCTTTC TGTGCAGTAT CGGCTGAGAC ATCGGCTACC AAGCAAACAG 
CAACCATCTG ATGTTTCATA TAATGATCTT CTCAAGCAAC TTACAGCTCA GCAGCATGCT 
ATTCTTGTGG ATTTAGGAAG GACCTTCCCC ACCCATCCCT ACTTCTCTGC CCAGCTGGGA 
GCAGGACAGT TATCACTGTA TAATCTTCTC AAAGCATACT CATTATTAGA CAAAGAAGTT 
GGTTACTGCC AGGGAATAAG CTTTGTGGCC GGTGTACTTC TCCTGCACAT GAGTGAACAG 
CAAGCTTTTG AAATGCTGAA ATTTCTCATG TATGATCTTG GCTTCCGCAA GCAGTACCGC 
CCTGATATGA TGTCGCTACA GATTCAGATG TACCAGCTTT CCCGGCTTCT CCACGACTAC 
CACCGAGATC TTTATAACCA CCTGGAGGAG AATGAAATCA ACCCAAGCCT CTATGCAGCC 
CCTTGGTTTC TCACACTTTT CGCTTCACAG TTTCCCTTAG GTTTTGTTGC TAGGGTTTTT 
GATATCATTT TTTTACAAGG GACTGAAGTC ATTTTCAAAG TGGCTCTTTG CTTGCTCAGT 
AATAGCGAAG CACTTATTAT GGCCCGGGAC AGCTTTGAGA GTATTGTGGA TTTCCTGAAG 
AGCATGATAC CAGAAATGAC AAATGAAACC ATGGAAAAAA TTATTACACA GGTTTTTGAG 
ATGGACATTT CAAAGCAACT CCATGCTTAT GAGGTTGAGT ACCATGTCAT AAATGATGAG 
ATGCTGGAGG CTTCCATTCC TTCTGATGAC AATGAGTCCT TCCACAAGTT GGAAAAATCC 
AATAATCAGC TTAAGAGACA GAACATGGAT TTACTGGAAA AACTTCAGGT AGCACATACG 
AAGATTCAGA GCTTGGAATC CAATGTGGAA AATATTTTGT CCAGAGAGAA TAAACTGAGA 
AATGTAGTCC GATCGATGGA AGAGGAAAGA TCAGCTTATC AGAATGTGTT TGAGCTCATG 
AAAAAATATG TGCCGACCAA TGTTCTGACA GACTGTGAAC TCTTGCTGAA AAATACCACA 
TGTAGTCCTA ACAACAGGAC CAAGGCTGGA AACAAGCAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004911887.1
CDS	1148..4969
Translation	MERRSSADLCHPLLEVNTMPDKVLKEEKRFHLWYIGWSSLDRRTTLPMLPWLVAEIRRRAERQDPPGCAAPCREVNLLLVTPHIRCVPALNGANANSAVFIFEHKAQHISRFIHNSQDPSYFAYLIKTQPDNPESEMACHVFKANTPRQVSDIISSIRQVSKVALKEDSKPCRDSEDAFYNSQKFEVLYCGKVVIANKKAPSTLVDDCIEKFSQHERQRLKSLNEQQSAECSTDFSLAEIDVPGSPSDMFLEQDSENNFIPSSEHHSGMFTVGSQSNLIGSRVTFPERILEDSGFEDQQEFRTRCSSVTIHKRAQDNKIQPRRRHASAPSHVQPTDSEKNRTMLFQVGRFEINLISPDTKTVELEKNFKDISSCSQGIKHIDHFGLVCRECVEPGINQYICYVFQCASEALVEEVMLTLKQAFSTAAALQSAKTQIKLCEACPMHALHKLCERIEGLYPPRAKLVIQKQLSLLTDNEQADIFERVQKMKATSDQEENELVILHLRQLCETKQKTHVHIGEVQQNLPCNTVPENAAPSGRFKLDILKNKAKKSLTSSLENIFSRGTNKMRGRLGSMDSFERCNSFTSDKDGSPGDSPPATPPSSPVSSSCPPFPEEDPDSPKVRRRAHTFSHPTSSGKRRITFPDSKSPSARFPLQRQNSLANDVGQSSPVPIARRFRTESESSSSSLPSSFSAPSFLKNFYQNSGRHVPQSENDTPKSESEGRRRTFSSCSNDSLNASGSCLIPHRISWRQRIFLRVASPMTNSLSDMQHKGQLDVNDLLPLSPLPPAMEEDPLAALQAEDSALQSEGKRSSEDFQNLWKKAIHQQILLLRMEKENMKLEASRDELQSRKVKLDYEEVGLCQKEVLNLWDKKLNCRTKIRFDMEEIFATLKEGVPKGKRGEIWQFLSVQYRLRHRLPSKQQPSDVSYNDLLKQLTAQQHAILVDLGRTFPTHPYFSAQLGAGQLSLYNLLKAYSLLDKEVGYCQGISFVAGVLLLHMSEQQAFEMLKFLMYDLGFRKQYRPDMMSLQIQMYQLSRLLHDYHRDLYNHLEENEINPSLYAAPWFLTLFASQFPLGFVARVFDIIFLQGTEVIFKVALCLLSNSEALIMARDSFESIVDFLKSMIPEMTNETMEKIITQVFEMDISKQLHAYEVEYHVINDEMLEASIPSDDNESFHKLEKSNNQLKRQNMDLLEKLQVAHTKIQSLESNVENILSRENKLRNVVRSMEEERSAYQNVFELMKKYVPTNVLTDCELLLKNTTCSPNNRTKAGNKQ

Target ORF information:

RefSeq Version	XM_004911830.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis TBC1 domain family member 4 (tbc1d4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911830.3

ORF Insert Sequence:

ATGGAGCGAA GAAGTTCAGC AGACCTGTGC CACCCTCTTT TGGAAGTAAA TACAATGCCA 
GACAAGGTGC TCAAGGAGGA GAAAAGATTT CACTTGTGGT ACATTGGGTG GTCTTCCCTA 
GACCGTCGGA CAACCTTGCC AATGCTTCCA TGGCTTGTAG CTGAGATCCG CAGAAGGGCA 
GAAAGGCAAG ACCCTCCAGG ATGTGCGGCA CCTTGCAGAG AAGTCAATCT TTTGCTAGTT 
ACCCCACACA TCCGCTGTGT GCCAGCGTTG AATGGCGCCA ATGCCAACAG TGCCGTCTTC 
ATCTTTGAGC ACAAAGCACA GCATATATCC CGCTTCATCC ACAATAGCCA AGATCCTTCT 
TATTTTGCCT ATCTGATTAA GACTCAGCCT GACAACCCTG AATCTGAAAT GGCATGCCAT 
GTGTTCAAGG CCAATACCCC AAGACAGGTT TCTGATATCA TCAGTAGCAT ACGTCAAGTC 
TCCAAAGTTG CACTGAAAGA AGATTCCAAG CCTTGCAGAG ATAGTGAAGA TGCCTTTTAT 
AATTCACAGA AGTTTGAAGT GTTATACTGT GGGAAAGTAG TAATTGCAAA TAAAAAAGCA 
CCATCTACCC TTGTTGATGA CTGTATTGAG AAATTCAGCC AACATGAACG CCAGCGTCTA 
AAGTCATTAA ATGAACAGCA GAGCGCAGAA TGCAGCACTG ATTTTTCTTT AGCAGAAATC 
GATGTCCCAG GTTCCCCTTC TGATATGTTT CTGGAGCAGG ACTCTGAAAA TAATTTCATC 
CCTTCTTCTG AGCATCATTC AGGGATGTTT ACAGTTGGCA GTCAAAGCAA TCTGATTGGT 
TCACGAGTAA CATTTCCTGA GAGGATTTTA GAAGACTCTG GCTTTGAGGA TCAGCAAGAA 
TTTAGAACTC GGTGCAGTAG TGTTACAATT CACAAAAGAG CCCAGGATAA TAAAATACAG 
CCAAGAAGAA GGCATGCAAG TGCCCCAAGC CATGTTCAAC CCACTGATTC AGAGAAGAAT 
AGAACAATGC TCTTTCAGGT TGGACGTTTT GAAATCAATC TCATAAGCCC TGATACTAAA 
ACCGTGGAGC TTGAGAAGAA TTTCAAAGAT ATATCATCAT GTTCTCAGGG TATTAAACAC 
ATTGATCATT TTGGGTTGGT CTGCCGTGAA TGTGTGGAGC CTGGGATAAA TCAGTACATT 
TGTTATGTTT TTCAGTGTGC ATCTGAAGCA TTGGTGGAGG AAGTCATGCT TACTTTGAAG 
CAGGCATTCA GCACTGCTGC TGCCTTACAG AGTGCAAAGA CCCAGATCAA GCTATGTGAA 
GCGTGCCCTA TGCATGCCTT GCACAAGCTT TGTGAACGAA TTGAAGGCCT TTACCCACCA 
AGAGCAAAGC TTGTAATACA AAAGCAGCTG TCTTTGCTGA CTGATAACGA ACAAGCAGAT 
ATCTTTGAAC GTGTCCAGAA AATGAAGGCC ACCAGTGACC AAGAAGAAAA TGAGCTTGTG 
ATTCTGCATC TAAGGCAGTT ATGTGAGACA AAACAAAAGA CCCATGTTCA TATAGGAGAA 
GTGCAACAGA ATCTGCCATG CAACACAGTT CCAGAAAATG CAGCTCCAAG TGGCAGATTT 
AAACTGGATA TTCTGAAGAA CAAGGCTAAG AAATCCTTGA CCAGCTCTTT GGAAAATATC 
TTTTCAAGGG GAACAAATAA AATGAGAGGA CGATTGGGCA GTATGGACAG TTTTGAACGT 
TGTAACAGTT TTACATCTGA CAAGGACGGA TCACCCGGGG ACTCACCTCC TGCCACACCA 
CCATCTTCCC CTGTGTCCTC TTCCTGCCCG CCATTTCCAG AGGAAGATCC CGACTCACCT 
AAGGTTCGCA GACGAGCTCA CACTTTCAGC CACCCCACAT CCAGTGGCAA GCGGAGGATA 
ACATTTCCTG ACAGTAAATC ACCGAGTGCC AGGTTCCCCC TCCAGAGACA GAATTCCTTA 
GCAAATGATG TGGGACAAAG CAGCCCAGTT CCTATCGCTC GGCGATTTCG CACTGAGAGT 
GAGTCCTCGT CCTCCAGTCT CCCCTCATCC TTCTCTGCCC CTTCTTTTCT GAAAAATTTT 
TACCAGAATT CAGGGAGACA TGTCCCACAA TCTGAGAATG ACACCCCCAA GAGTGAAAGC 
GAAGGACGAA GGAGAACATT TTCTTCATGT AGCAACGACT CATTAAATGC TAGTGGGTCC 
TGTCTAATAC CACATCGAAT ATCGTGGCGG CAGCGGATTT TCCTCAGGGT AGCATCTCCA 
ATGACCAACT CTCTATCAGA TATGCAGCAT AAAGGCCAGC TCGATGTGAA CGACTTGCTG 
CCATTATCTC CTCTTCCTCC TGCAATGGAA GAAGACCCTC TAGCTGCTCT TCAGGCAGAA 
GACTCGGCAC TACAATCAGA AGGGAAGCGA AGCTCTGAAG ACTTTCAGAA CCTGTGGAAG 
AAAGCAATAC ATCAGCAGAT ATTGTTACTA CGGATGGAAA AGGAGAATAT GAAGCTTGAA 
GCAAGCAGAG ATGAACTGCA ATCCCGGAAA GTCAAATTGG ACTATGAAGA AGTTGGATTG 
TGCCAAAAAG AAGTCTTAAA TCTTTGGGAT AAGAAGTTAA ACTGCAGAAC AAAGATTCGG 
TTTGATATGG AAGAGATTTT TGCCACTCTT AAAGAAGGTG TACCGAAAGG CAAGCGTGGA 
GAGATATGGC AGTTCCTTTC TGTGCAGTAT CGGCTGAGAC ATCGGCTACC AAGCAAACAG 
CAACCATCTG ATGTTTCATA TAATGATCTT CTCAAGCAAC TTACAGCTCA GCAGCATGCT 
ATTCTTGTGG ATTTAGGAAG GACCTTCCCC ACCCATCCCT ACTTCTCTGC CCAGCTGGGA 
GCAGGACAGT TATCACTGTA TAATCTTCTC AAAGCATACT CATTATTAGA CAAAGAAGTT 
GGTTACTGCC AGGGAATAAG CTTTGTGGCC GGTGTACTTC TCCTGCACAT GAGTGAACAG 
CAAGCTTTTG AAATGCTGAA ATTTCTCATG TATGATCTTG GCTTCCGCAA GCAGTACCGC 
CCTGATATGA TGTCGCTACA GATTCAGATG TACCAGCTTT CCCGGCTTCT CCACGACTAC 
CACCGAGATC TTTATAACCA CCTGGAGGAG AATGAAATCA ACCCAAGCCT CTATGCAGCC 
CCTTGGTTTC TCACACTTTT CGCTTCACAG TTTCCCTTAG GTTTTGTTGC TAGGGTTTTT 
GATATCATTT TTTTACAAGG GACTGAAGTC ATTTTCAAAG TGGCTCTTTG CTTGCTCAGT 
AATAGCGAAG CACTTATTAT GGCCCGGGAC AGCTTTGAGA GTATTGTGGA TTTCCTGAAG 
AGCATGATAC CAGAAATGAC AAATGAAACC ATGGAAAAAA TTATTACACA GGTTTTTGAG 
ATGGACATTT CAAAGCAACT CCATGCTTAT GAGGTTGAGT ACCATGTCAT AAATGATGAG 
ATGCTGGAGG CTTCCATTCC TTCTGATGAC AATGAGTCCT TCCACAAGTT GGAAAAATCC 
AATAATCAGC TTAAGAGACA GAACATGGAT TTACTGGAAA AACTTCAGGT AGCACATACG 
AAGATTCAGA GCTTGGAATC CAATGTGGAA AATATTTTGT CCAGAGAGAA TAAACTGAGA 
AATGTAGTCC GATCGATGGA AGAGGAAAGA TCAGCTTATC AGAATGTGTT TGAGCTCATG 
AAAAAATATG TGCCGACCAA TGTTCTGACA GACTGTGAAC TCTTGCTGAA AAATACCACA 
TGTAGTCCTA ACAACAGGAC CAAGGCTGGA AACAAGCAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tbc1d4 ( XM_002931938.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002931984.2 Xenopus tropicalis TBC1 domain family member 4 (tbc1d4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa05929
Clone ID Related Accession (Same CDS sequence)	XM_002931938.4
Accession Version	XM_002931938.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	TBC1 domain family member 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002931938.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGAA GAAGTTCAGC AGACCTGTGC CACCCTCTTT TGGAAGTAAA TACAATGCCA 
GACAAGGTGC TCAAGGAGGA GAAAAGATTT CACTTGTGGT ACATTGGGTG GTCTTCCCTA 
GACCGTCGGA CAACCTTGCC AATGCTTCCA TGGCTTGTAG CTGAGATCCG CAGAAGGGCA 
GAAAGGCAAG ACCCTCCAGG ATGTGCGGCA CCTTGCAGAG AAGTCAATCT TTTGCTAGTT 
ACCCCACACA TCCGCTGTGT GCCAGCGTTG AATGGCGCCA ATGCCAACAG TGCCGTCTTC 
ATCTTTGAGC ACAAAGCACA GCATATATCC CGCTTCATCC ACAATAGCCA AGATCCTTCT 
TATTTTGCCT ATCTGATTAA GACTCAGCCT GACAACCCTG AATCTGAAAT GGCATGCCAT 
GTGTTCAAGG CCAATACCCC AAGACAGGTT TCTGATATCA TCAGTAGCAT ACGTCAAGTC 
TCCAAAGTTG CACTGAAAGA AGATTCCAAG CCTTGCAGAG ATAGTGAAGA TGCCTTTTAT 
AATTCACAGA AGTTTGAAGT GTTATACTGT GGGAAAGTAG TAATTGCAAA TAAAAAAGCA 
CCATCTACCC TTGTTGATGA CTGTATTGAG AAATTCAGCC AACATGAACG CCAGCGTCTA 
AAGTCATTAA ATGAACAGCA GAGCGCAGAA TGCAGCACTG ATTTTTCTTT AGCAGAAATC 
GATGTCCCAG GTTCCCCTTC TGATATGTTT CTGGAGCAGG ACTCTGAAAA TAATTTCATC 
CCTTCTTCTG AGCATCATTC AGGGATGTTT ACAGTTGGCA GTCAAAGCAA TCTGATTGGT 
TCACGAGTAA CATTTCCTGA GAGGATTTTA GAAGACTCTG GCTTTGAGGA TCAGCAAGAA 
TTTAGAACTC GGTGCAGTAG TGTTACAATT CACAAAAGAG CCCAGGATAA TAAAATACAG 
CCAAGAAGAA GGCATGCAAG TGCCCCAAGC CATGTTCAAC CCACTGATTC AGAGAAGAAT 
AGAACAATGC TCTTTCAGGT TGGACGTTTT GAAATCAATC TCATAAGCCC TGATACTAAA 
ACCGTGGAGC TTGAGAAGAA TTTCAAAGAT ATATCATCAT GTTCTCAGGG TATTAAACAC 
ATTGATCATT TTGGGTTGGT CTGCCGTGAA TGTGTGGAGC CTGGGATAAA TCAGTACATT 
TGTTATGTTT TTCAGTGTGC ATCTGAAGCA TTGGTGGAGG AAGTCATGCT TACTTTGAAG 
CAGGCATTCA GCACTGCTGC TGCCTTACAG AGTGCAAAGA CCCAGATCAA GCTATGTGAA 
GCGTGCCCTA TGCATGCCTT GCACAAGCTT TGTGAACGAA TTGAAGGCCT TTACCCACCA 
AGAGCAAAGC TTGTAATACA AAAGCAGCTG TCTTTGCTGA CTGATAACGA ACAAGCAGAT 
ATCTTTGAAC GTGTCCAGAA AATGAAGGCC ACCAGTGACC AAGAAGAAAA TGAGCTTGTG 
ATTCTGCATC TAAGGCAGTT ATGTGAGACA AAACAAAAGA CCCATGTTCA TATAGGAGAA 
GTGCAACAGA ATCTGCCATG CAACACAGTT CCAGAAAATG CAGCTCCAAG TGGCAGATTT 
AAACTGGATA TTCTGAAGAA CAAGGCTAAG AAATCCTTGA CCAGCTCTTT GGAAAATATC 
TTTTCAAGGG GAACAAATAA AATGAGAGGA CGATTGGGCA GTATGGACAG TTTTGAACGT 
TGTAACAGTT TTACATCTGA CAAGGACGGA TCACCCGGGG ACTCACCTCC TGCCACACCA 
CCATCTTCCC CTGTGTCCTC TTCCTGCCCG CCATTTCCAG AGGAAGATCC CGACTCACCT 
AAGGTTCGCA GACGAGCTCA CACTTTCAGC CACCCCACAT CCAGTGGCAA GCGGAGGATA 
ACATTTCCTG ACAGTAAATC ACCGAGTGCC AGGTTCCCCC TCCAGAGACA GAATTCCTTA 
GCAAATGATG TGGGACAAAG CAGTGAAAGC GAAGGACGAA GGAGAACATT TTCTTCATGT 
AGCAACGACT CATTAAATGC TAGTGGGTCC TGTCTAATAC CACATCGAAT ATCGTGGCGG 
CAGCGGATTT TCCTCAGGGT AGCATCTCCA ATGACCAACT CTCTATCAGA TATGCAGCAT 
AAAGGCCAGC TCGATGTGAA CGACTTGCTG CCATTATCTC CTCTTCCTCC TGCAATGGAA 
GAAGACCCTC TAGCTGCTCT TCAGGCAGAA GACTCGGCAC TACAATCAGA AGGGAAGCGA 
AGCTCTGAAG ACTTTCAGAA CCTGTGGAAG AAAGCAATAC ATCAGCAGAT ATTGTTACTA 
CGGATGGAAA AGGAGAATAT GAAGCTTGAA GCAAGCAGAG ATGAACTGCA ATCCCGGAAA 
GTCAAATTGG ACTATGAAGA AGTTGGATTG TGCCAAAAAG AAGTCTTAAA TCTTTGGGAT 
AAGAAGTTAA ACTGCAGAAC AAAGATTCGG TTTGATATGG AAGAGATTTT TGCCACTCTT 
AAAGAAGGTG TACCGAAAGG CAAGCGTGGA GAGATATGGC AGTTCCTTTC TGTGCAGTAT 
CGGCTGAGAC ATCGGCTACC AAGCAAACAG CAACCATCTG ATGTTTCATA TAATGATCTT 
CTCAAGCAAC TTACAGCTCA GCAGCATGCT ATTCTTGTGG ATTTAGGAAG GACCTTCCCC 
ACCCATCCCT ACTTCTCTGC CCAGCTGGGA GCAGGACAGT TATCACTGTA TAATCTTCTC 
AAAGCATACT CATTATTAGA CAAAGAAGTT GGTTACTGCC AGGGAATAAG CTTTGTGGCC 
GGTGTACTTC TCCTGCACAT GAGTGAACAG CAAGCTTTTG AAATGCTGAA ATTTCTCATG 
TATGATCTTG GCTTCCGCAA GCAGTACCGC CCTGATATGA TGTCGCTACA GATTCAGATG 
TACCAGCTTT CCCGGCTTCT CCACGACTAC CACCGAGATC TTTATAACCA CCTGGAGGAG 
AATGAAATCA ACCCAAGCCT CTATGCAGCC CCTTGGTTTC TCACACTTTT CGCTTCACAG 
TTTCCCTTAG GTTTTGTTGC TAGGGTTTTT GATATCATTT TTTTACAAGG GACTGAAGTC 
ATTTTCAAAG TGGCTCTTTG CTTGCTCAGT AATAGCGAAG CACTTATTAT GGCCCGGGAC 
AGCTTTGAGA GTATTGTGGA TTTCCTGAAG AGCATGATAC CAGAAATGAC AAATGAAACC 
ATGGAAAAAA TTATTACACA GGTTTTTGAG ATGGACATTT CAAAGCAACT CCATGCTTAT 
GAGGTTGAGT ACCATGTCAT AAATGATGAG ATGCTGGAGG CTTCCATTCC TTCTGATGAC 
AATGAGTCCT TCCACAAGTT GGAAAAATCC AATAATCAGC TTAAGAGACA GAACATGGAT 
TTACTGGAAA AACTTCAGGT AGCACATACG AAGATTCAGA GCTTGGAATC CAATGTGGAA 
AATATTTTGT CCAGAGAGAA TAAACTGAGA AATGTAGTCC GATCGATGGA AGAGGAAAGA 
TCAGCTTATC AGAATGTGTT TGAGCTCATG AAAAAATATG TGCCGACCAA TGTTCTGACA 
GACTGTGAAC TCTTGCTGAA AAATACCACA TGTAGTCCTA ACAACAGGAC CAAGGCTGGA 
AACAAGCAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002931984.2
CDS	1292..4963
Translation	MERRSSADLCHPLLEVNTMPDKVLKEEKRFHLWYIGWSSLDRRTTLPMLPWLVAEIRRRAERQDPPGCAAPCREVNLLLVTPHIRCVPALNGANANSAVFIFEHKAQHISRFIHNSQDPSYFAYLIKTQPDNPESEMACHVFKANTPRQVSDIISSIRQVSKVALKEDSKPCRDSEDAFYNSQKFEVLYCGKVVIANKKAPSTLVDDCIEKFSQHERQRLKSLNEQQSAECSTDFSLAEIDVPGSPSDMFLEQDSENNFIPSSEHHSGMFTVGSQSNLIGSRVTFPERILEDSGFEDQQEFRTRCSSVTIHKRAQDNKIQPRRRHASAPSHVQPTDSEKNRTMLFQVGRFEINLISPDTKTVELEKNFKDISSCSQGIKHIDHFGLVCRECVEPGINQYICYVFQCASEALVEEVMLTLKQAFSTAAALQSAKTQIKLCEACPMHALHKLCERIEGLYPPRAKLVIQKQLSLLTDNEQADIFERVQKMKATSDQEENELVILHLRQLCETKQKTHVHIGEVQQNLPCNTVPENAAPSGRFKLDILKNKAKKSLTSSLENIFSRGTNKMRGRLGSMDSFERCNSFTSDKDGSPGDSPPATPPSSPVSSSCPPFPEEDPDSPKVRRRAHTFSHPTSSGKRRITFPDSKSPSARFPLQRQNSLANDVGQSSESEGRRRTFSSCSNDSLNASGSCLIPHRISWRQRIFLRVASPMTNSLSDMQHKGQLDVNDLLPLSPLPPAMEEDPLAALQAEDSALQSEGKRSSEDFQNLWKKAIHQQILLLRMEKENMKLEASRDELQSRKVKLDYEEVGLCQKEVLNLWDKKLNCRTKIRFDMEEIFATLKEGVPKGKRGEIWQFLSVQYRLRHRLPSKQQPSDVSYNDLLKQLTAQQHAILVDLGRTFPTHPYFSAQLGAGQLSLYNLLKAYSLLDKEVGYCQGISFVAGVLLLHMSEQQAFEMLKFLMYDLGFRKQYRPDMMSLQIQMYQLSRLLHDYHRDLYNHLEENEINPSLYAAPWFLTLFASQFPLGFVARVFDIIFLQGTEVIFKVALCLLSNSEALIMARDSFESIVDFLKSMIPEMTNETMEKIITQVFEMDISKQLHAYEVEYHVINDEMLEASIPSDDNESFHKLEKSNNQLKRQNMDLLEKLQVAHTKIQSLESNVENILSRENKLRNVVRSMEEERSAYQNVFELMKKYVPTNVLTDCELLLKNTTCSPNNRTKAGNKQ

Target ORF information:

RefSeq Version	XM_002931938.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis TBC1 domain family member 4 (tbc1d4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002931938.4

ORF Insert Sequence:

ATGGAGCGAA GAAGTTCAGC AGACCTGTGC CACCCTCTTT TGGAAGTAAA TACAATGCCA 
GACAAGGTGC TCAAGGAGGA GAAAAGATTT CACTTGTGGT ACATTGGGTG GTCTTCCCTA 
GACCGTCGGA CAACCTTGCC AATGCTTCCA TGGCTTGTAG CTGAGATCCG CAGAAGGGCA 
GAAAGGCAAG ACCCTCCAGG ATGTGCGGCA CCTTGCAGAG AAGTCAATCT TTTGCTAGTT 
ACCCCACACA TCCGCTGTGT GCCAGCGTTG AATGGCGCCA ATGCCAACAG TGCCGTCTTC 
ATCTTTGAGC ACAAAGCACA GCATATATCC CGCTTCATCC ACAATAGCCA AGATCCTTCT 
TATTTTGCCT ATCTGATTAA GACTCAGCCT GACAACCCTG AATCTGAAAT GGCATGCCAT 
GTGTTCAAGG CCAATACCCC AAGACAGGTT TCTGATATCA TCAGTAGCAT ACGTCAAGTC 
TCCAAAGTTG CACTGAAAGA AGATTCCAAG CCTTGCAGAG ATAGTGAAGA TGCCTTTTAT 
AATTCACAGA AGTTTGAAGT GTTATACTGT GGGAAAGTAG TAATTGCAAA TAAAAAAGCA 
CCATCTACCC TTGTTGATGA CTGTATTGAG AAATTCAGCC AACATGAACG CCAGCGTCTA 
AAGTCATTAA ATGAACAGCA GAGCGCAGAA TGCAGCACTG ATTTTTCTTT AGCAGAAATC 
GATGTCCCAG GTTCCCCTTC TGATATGTTT CTGGAGCAGG ACTCTGAAAA TAATTTCATC 
CCTTCTTCTG AGCATCATTC AGGGATGTTT ACAGTTGGCA GTCAAAGCAA TCTGATTGGT 
TCACGAGTAA CATTTCCTGA GAGGATTTTA GAAGACTCTG GCTTTGAGGA TCAGCAAGAA 
TTTAGAACTC GGTGCAGTAG TGTTACAATT CACAAAAGAG CCCAGGATAA TAAAATACAG 
CCAAGAAGAA GGCATGCAAG TGCCCCAAGC CATGTTCAAC CCACTGATTC AGAGAAGAAT 
AGAACAATGC TCTTTCAGGT TGGACGTTTT GAAATCAATC TCATAAGCCC TGATACTAAA 
ACCGTGGAGC TTGAGAAGAA TTTCAAAGAT ATATCATCAT GTTCTCAGGG TATTAAACAC 
ATTGATCATT TTGGGTTGGT CTGCCGTGAA TGTGTGGAGC CTGGGATAAA TCAGTACATT 
TGTTATGTTT TTCAGTGTGC ATCTGAAGCA TTGGTGGAGG AAGTCATGCT TACTTTGAAG 
CAGGCATTCA GCACTGCTGC TGCCTTACAG AGTGCAAAGA CCCAGATCAA GCTATGTGAA 
GCGTGCCCTA TGCATGCCTT GCACAAGCTT TGTGAACGAA TTGAAGGCCT TTACCCACCA 
AGAGCAAAGC TTGTAATACA AAAGCAGCTG TCTTTGCTGA CTGATAACGA ACAAGCAGAT 
ATCTTTGAAC GTGTCCAGAA AATGAAGGCC ACCAGTGACC AAGAAGAAAA TGAGCTTGTG 
ATTCTGCATC TAAGGCAGTT ATGTGAGACA AAACAAAAGA CCCATGTTCA TATAGGAGAA 
GTGCAACAGA ATCTGCCATG CAACACAGTT CCAGAAAATG CAGCTCCAAG TGGCAGATTT 
AAACTGGATA TTCTGAAGAA CAAGGCTAAG AAATCCTTGA CCAGCTCTTT GGAAAATATC 
TTTTCAAGGG GAACAAATAA AATGAGAGGA CGATTGGGCA GTATGGACAG TTTTGAACGT 
TGTAACAGTT TTACATCTGA CAAGGACGGA TCACCCGGGG ACTCACCTCC TGCCACACCA 
CCATCTTCCC CTGTGTCCTC TTCCTGCCCG CCATTTCCAG AGGAAGATCC CGACTCACCT 
AAGGTTCGCA GACGAGCTCA CACTTTCAGC CACCCCACAT CCAGTGGCAA GCGGAGGATA 
ACATTTCCTG ACAGTAAATC ACCGAGTGCC AGGTTCCCCC TCCAGAGACA GAATTCCTTA 
GCAAATGATG TGGGACAAAG CAGTGAAAGC GAAGGACGAA GGAGAACATT TTCTTCATGT 
AGCAACGACT CATTAAATGC TAGTGGGTCC TGTCTAATAC CACATCGAAT ATCGTGGCGG 
CAGCGGATTT TCCTCAGGGT AGCATCTCCA ATGACCAACT CTCTATCAGA TATGCAGCAT 
AAAGGCCAGC TCGATGTGAA CGACTTGCTG CCATTATCTC CTCTTCCTCC TGCAATGGAA 
GAAGACCCTC TAGCTGCTCT TCAGGCAGAA GACTCGGCAC TACAATCAGA AGGGAAGCGA 
AGCTCTGAAG ACTTTCAGAA CCTGTGGAAG AAAGCAATAC ATCAGCAGAT ATTGTTACTA 
CGGATGGAAA AGGAGAATAT GAAGCTTGAA GCAAGCAGAG ATGAACTGCA ATCCCGGAAA 
GTCAAATTGG ACTATGAAGA AGTTGGATTG TGCCAAAAAG AAGTCTTAAA TCTTTGGGAT 
AAGAAGTTAA ACTGCAGAAC AAAGATTCGG TTTGATATGG AAGAGATTTT TGCCACTCTT 
AAAGAAGGTG TACCGAAAGG CAAGCGTGGA GAGATATGGC AGTTCCTTTC TGTGCAGTAT 
CGGCTGAGAC ATCGGCTACC AAGCAAACAG CAACCATCTG ATGTTTCATA TAATGATCTT 
CTCAAGCAAC TTACAGCTCA GCAGCATGCT ATTCTTGTGG ATTTAGGAAG GACCTTCCCC 
ACCCATCCCT ACTTCTCTGC CCAGCTGGGA GCAGGACAGT TATCACTGTA TAATCTTCTC 
AAAGCATACT CATTATTAGA CAAAGAAGTT GGTTACTGCC AGGGAATAAG CTTTGTGGCC 
GGTGTACTTC TCCTGCACAT GAGTGAACAG CAAGCTTTTG AAATGCTGAA ATTTCTCATG 
TATGATCTTG GCTTCCGCAA GCAGTACCGC CCTGATATGA TGTCGCTACA GATTCAGATG 
TACCAGCTTT CCCGGCTTCT CCACGACTAC CACCGAGATC TTTATAACCA CCTGGAGGAG 
AATGAAATCA ACCCAAGCCT CTATGCAGCC CCTTGGTTTC TCACACTTTT CGCTTCACAG 
TTTCCCTTAG GTTTTGTTGC TAGGGTTTTT GATATCATTT TTTTACAAGG GACTGAAGTC 
ATTTTCAAAG TGGCTCTTTG CTTGCTCAGT AATAGCGAAG CACTTATTAT GGCCCGGGAC 
AGCTTTGAGA GTATTGTGGA TTTCCTGAAG AGCATGATAC CAGAAATGAC AAATGAAACC 
ATGGAAAAAA TTATTACACA GGTTTTTGAG ATGGACATTT CAAAGCAACT CCATGCTTAT 
GAGGTTGAGT ACCATGTCAT AAATGATGAG ATGCTGGAGG CTTCCATTCC TTCTGATGAC 
AATGAGTCCT TCCACAAGTT GGAAAAATCC AATAATCAGC TTAAGAGACA GAACATGGAT 
TTACTGGAAA AACTTCAGGT AGCACATACG AAGATTCAGA GCTTGGAATC CAATGTGGAA 
AATATTTTGT CCAGAGAGAA TAAACTGAGA AATGTAGTCC GATCGATGGA AGAGGAAAGA 
TCAGCTTATC AGAATGTGTT TGAGCTCATG AAAAAATATG TGCCGACCAA TGTTCTGACA 
GACTGTGAAC TCTTGCTGAA AAATACCACA TGTAGTCCTA ACAACAGGAC CAAGGCTGGA 
AACAAGCAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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tbc1d4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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