pola1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	pola1
Entrez Gene ID	100379926
Full Name	polymerase (DNA directed), alpha 1, catalytic subunit
Synonyms	pola
General protein information	Preferred Names polymerase (DNA directed), alpha 1, catalytic subunit Names DNA polymerase alpha catalytic subunit DNA polymerase alpha
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002933612.4	XP_002933658.1	DNA polymerase alpha catalytic subunit isoform X2
XM_018091470.1	XP_017946959.1	DNA polymerase alpha catalytic subunit isoform X3
XM_012957632.2	XP_012813086.1	DNA polymerase alpha catalytic subunit isoform X1
XM_004911754.3	XP_004911811.1	DNA polymerase alpha catalytic subunit isoform X2
XM_031896703.1	XP_031752563.1	DNA polymerase alpha catalytic subunit isoform X3
XM_031896704.1	XP_031752564.1	DNA polymerase alpha catalytic subunit isoform X3
XM_031896702.1	XP_031752562.1	DNA polymerase alpha catalytic subunit isoform X1
XM_012957632.3	XP_012813086.2	DNA polymerase alpha catalytic subunit isoform X2

Pathways from BioSystems

Homologies

	POLA1	XP_001091195.1
Gallus gallus (chicken)	POLA1	XP_416792.3
Eremothecium gossypii	AGOS_AER381C	NP_985237.1
Homo sapiens (human)	POLA1	NP_058633.2
Rattus norvegicus (Norway rat)	Pola1	NP_445931.1
Schizosaccharomyces pombe (fission yeast)	pol1	NP_594186.1
Pan troglodytes (chimpanzee)	POLA1	XP_003317445.2
Mus musculus (house mouse)	Pola1	NP_032918.1
Danio rerio (zebrafish)	pola1	XP_005162809.1
Drosophila melanogaster (fruit fly)	DNApol-alpha180	NP_536736.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001929	XP_321134.5
Caenorhabditis elegans (roundworm)	Y47D3A.29	NP_001255109.1
Magnaporthe oryzae (rice blast fungus)	MGG_06397	XP_003717174.1
Neurospora crassa	NCU07870	XP_963131.2
Arabidopsis thaliana (thale cress)	ICU2	NP_201511.2
Xenopus tropicalis (tropical clawed frog)	pola1	XP_004911812.1
Bos taurus (cattle)	POLA1	NP_001192994.1
Saccharomyces cerevisiae (baker's yeast)	POL1	NP_014297.3
Kluyveromyces lactis	KLLA0C13255g	XP_452791.1
Canis lupus familiaris (dog)	POLA1	XP_005641271.1
Oryza sativa (rice)	Os01g0868300	NP_001044920.1

Gene Ontology

The following pola1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pola1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa42678	XM_018091470.1 Latest version!	Xenopus tropicalis polymerase (DNA directed), alpha 1, catalytic subunit (pola1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OXa52095	XM_031896702.1 Latest version!	Xenopus tropicalis polymerase (DNA directed), alpha 1, catalytic subunit (pola1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa52096	XM_012957632.3 Latest version!	Xenopus tropicalis polymerase (DNA directed), alpha 1, catalytic subunit (pola1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa52097	XM_031896703.1 Latest version!	Xenopus tropicalis polymerase (DNA directed), alpha 1, catalytic subunit (pola1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa52097	XM_031896704.1 Latest version!	Xenopus tropicalis polymerase (DNA directed), alpha 1, catalytic subunit (pola1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa05773	XM_002933612.4 Latest version!	Xenopus tropicalis polymerase (DNA directed), alpha 1, catalytic subunit (pola1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa05773	XM_004911754.3 Latest version!	Xenopus tropicalis polymerase (DNA directed), alpha 1, catalytic subunit (pola1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa33001	XM_012957632.2 Latest version!	Xenopus tropicalis polymerase (DNA directed), alpha 1, catalytic subunit (pola1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pola1 ( XM_018091470.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017946959.1 Xenopus tropicalis polymerase (DNA directed), alpha 1, catalytic subunit (pola1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa42678
Clone ID Related Accession (Same CDS sequence)	XM_018091470.1
Accession Version	XM_018091470.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA polymerase alpha catalytic subunit isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTTGG TGTTCTGTGC GCCCCGCAGT ACCGTCTTCC CTCCTTACCT TGACATGTCA 
GAATCTGGAA GTTTTGCAGC ATCACGTTCG CGGCGTGAAA AAACAGAAAA GAGCGGCCGT 
AAAGAAGCAT TGGAACGTTT GAAAAGAGCA AAAGCTGGTG AAAAAGTCAA ATATGAGGTG 
GAAGAAATTT CAAGCATTTA TGAGGAGGTG GATGAAGAAC AATACTCTAA AATTGTTCGT 
GACCGACAGG ATGATGATTG GATAGTGGAT GATGATGGGA CCGGTTATGT AGAAGATGGA 
AGAGAGATTT TTGATGATGA CTTAGAAGAC AATGCTCTTG CTGATTCAGG TAAAAGAGCT 
AAGGGAGCAC CCAAAGATAA ATCTAATGTA AAAAAGTCAT CGGTGTCGAA GCCAAATAAT 
ATCAAGTCAA TGTTTATGGC CAGTGCTGTC AAAAGGACAA CAGATAAAAC TGTGGATCTG 
TCAAAAGATG ACTTGCTTGG TGATTTACTG CAAGATTTAA AGTCACAGGC TCTTCCTATA 
ACACCACCCC CAGTAATTAC TCTAAAGAAG AAGAAACTTG CAGGGTCTCC TTTAAACCCT 
TTCTCGGTAC CACCTGCTGC CCCAAAGGTT TTACCTGTGA AAAAACCACC AGCTTTAGCA 
AAGCTTGGGC ATCTCTCAAG TGCTGCGCCA TCTAACAACT CTGCCAGTTC TTTTGTACCC 
CGGGAAATTA AGAATGAGCC AAATTCTGAT TTGATTTCTT CTGCTCCTTT GAATGTGAAA 
ACTCAAGTAA AAGAAGAGGA TACGTCAATG GTTGAGTTCG ATGATGGAGA TTTTGATGAA 
CCAATGGAAG AAGACAATTT GGAATCTCCT TCAGTTGTTT CTAGCACAAT TAAGAAGGAA 
GAACCAAAAA TTAAGTGTGT GAAAGAGGAT AGTGTTAAAA TGGAGGAAAG TTCTTTCATG 
ACTTCCGCTG CTTTAAATGA ATCTTGTTGG GATCAAATTG ATGAGGTTGA ATCAATGACC 
ACTGAAGTTC AGGTAGATTC AAGCCGCCTA CCCTTGGTTA CAGGTGCAGA TGGAAGTCAG 
GTGTTTAAGT TTTATTGGCT AGATGCCTAT GAAGACCAGT ACAGTCAGCC AGGAGTAGTC 
TACTTATTTG GAAAAGTTTG GATAGAGACA GCTGAGGCGT ATGTCAGCTG CTGTGTTAGT 
GTGAAGAACA TTGAGAGGAC TGTATATCTC CTACCTCGTG AAAATAGAGT GCAGCTTTCT 
ACAGGCAAAG ACACTGGAGT ACCAGTGTCT ATGATGAATG TATACCAGGA GTTTAATGAA 
AGTGTTGCAG AAAAGTACAA AATCATGAAG TTCAAGTCTA AGAAAGTTGA GAAGGATTAT 
GCTTTTGAAA TTCCTGATGT TCCAGCCAGC TCTGAATATC TAGAGGTTCG ATATTCTGCG 
GACTCACCTC AACTTCCTCA GGATCTAAAA GGAGAAACAT TTTCACATGT GTTTGGAACA 
AATACTTCAA GCCTGGAGCT TTTCTTGCTT AGCAGAAAAA TAAAAGGGCC CAGCTGGCTG 
GAGATAAAAA GCCCACAGCT GAGCAGTCAG CCCATGAGCT GGTGCAAGGT TGAGGCAGTG 
GTGATGAGGC CTGACCAAGT GAATGTTGTG AAGGATCTGG CACCTCCACC TGTTGTTGTT 
TTGTCGCTCA GTATGAAGAC TGTGCAGAAT GCAAAGACTC ACCAAAATGA GATTGTGGCA 
ATGGCTGCTT TGGTTCATCA CAAATTTCCC TTTGATAAAG CACCACCACA GCCACCATTC 
CAGACACATT TTTGTGTTCT GAGCAAACCT AATGATTGCA TTTTCCCATA CGACTTTAAG 
GAAGCGGTGA AACGAAAGAA TGCAAATATT GAGATTGCGT TAACTGAACG AACTCTACTG 
GGCTTTTTCC TAGCAAAAAT TCATAAAATT GATCCAGATA TTATTGTAGG CCATGACATT 
TATGGTTTTG ATCTCGAAGT GTTGCTTCAA AGAATTAGCT CGTGCAAGGT TCCTTTCTGG 
TCCAAAATCG GTAGACTGAG AAGATCTGTT ATGCCAAAAC TTGGGGGTCG AAGTGGCTTT 
GCAGAAAGGA ATGCAGCTTG TGGTCGCATC ATTTGCGATA CTGAGATTTC TGCCAAGGAG 
TTAATTCGAT GTAAAAGCTA TCATTTGTCA GAACTGGTAC ATCAAATATT GAAAACAGAG 
AGAGTTTTGA TGCCACCAGA AAACATCCGC AATGCATACA GTGATTCAGC ACATCTTCTT 
TACATGCTGG AGAACACCTG GACTGATGCA AAATTCATCC TTCAGATCAT GTGTGAGCTG 
AATGTTCTTC CCCTGGCCTT GCAGATCACA AATATTGCAG GCAATGTCTT GTCAAGGACA 
TTAATGGGTG GACGCTCAGA AAGAAACGAG TACCTTCTCC TTCATGCCTT CACAGAAAAT 
AACTTCATAG TGCCAGACAA GCCAGTGTTC AAGAAAATTC AACAGACCAC TGTGGAGGAC 
GATGATAATA TGGACACAGA TCAAAATAAA AATAAGAAAG GTCGAAAGAA GGCTGCCTAT 
GCTGGAGGAT TGGTCCTGGA ACCCAAAGTT GGTTTCTACG ACAAATTCAT CTTGCTTCTG 
GATTTCAACA GCTTGTACCC CTCTATCATT CAGGAATATA ATATCTGCTT TACAACAGTG 
CAAAGAGAGG CTCCTACTTC ACAGAAAGGT GAAGATCAGG AAGAAATTCC AGAGCTACCT 
CATTCTGATC TTGAAATGGG GATCTTACCA CGAGAGATAC GGAAACTGGT GGAGAGGAGG 
CGTCACGTGA AGCAGTTAAT GAAGCAGCCT GACCTAAACC CTGATTTATA CTTACAGTAT 
GACATCAGAC AAAAAGCTTT AAAGCTCACG GCAAACAGCA TGTATGGATG CCTGGGCTTC 
TCCTACAGCA GGTTCTATGC CAAACCTTTG GCTGCATTGG TTACTCACCA AGGGAGAGAG 
ATTTTGCTGC ATACCAAGGA AATGGTACAG AAGATGAATC TGGAAGTCAT ATATGGAGAC 
ACGGATTCTA TCATGATAAA CACCAATTGT AATAATTTAG AAGAAGTCTT CAAGCTTGGA 
AACCGGGTGA AAAGTGAGGT AAACAAATCA TACAAACTTT TAGAGATTGA CATTGATGGT 
ATCTTTAAAT CACTCTTGCT ACTTAAGAAA AAGAAGTATG CCGCTCTGAC TGTGGAGCCT 
ACAGGTGATG GAAAGTATGT TACTAAGCAG GAGCTTAAAG GTTTGGATAT TGTCAGAAGA 
GACTGGTGTG AACTTGCTAA AGAAGCTGGA AATTATGTTA TCAGTCAGAT TCTGTCTGAT 
CAGCCACGTG ACAATATTGT TGAGAACATT CAGAAGAAAT TAACTGAAAT TGGGGAGAAT 
GTAACAAATG GAACTGTGCC CATTAGCCAG TATGAAATAA ACAAGGCATT GACAAAGGAT 
CCTCAGGATT ACCCTGATAA AAAAAGCTTA CCTCACGTAC ATGTTGCCAT GTGGATAAAT 
TCCCAAGGTG GAAGAAAGGT GAAAGCTGGT GATACAATAT CCTATGTCAT TTGCCAGGAC 
GGATCAAATC TTAGTGCCAG CCAGAGAGCA TATGCTCCAG AGCAGCTGCA AAAACAAGAG 
AACCTTTCCA TTGACACACA ATACTACCTG TCACAGCAAG TCCACCCAGT AGTGGCTCGG 
ATCTGTGAGC CCATTGATGG AATTGATTCT GCACTCATTG CAATGTGGTT AGGACTTGAC 
CCCTCCCAGT TCCGGGCTCA CCGCCACCAT CAGCAGGATG AGGAAAATGA TGCATTGTTG 
GGAGGTCCTT CCCAACTCAC AGATGAGGAA AAATACAGAG ATTGTGAACG ATTCAAATTC 
TTCTGCCCAA AGTGTGGAAC AGAGAACATT TATGACAATG TTTTTGATGG CTCTGGGCTG 
CAGATTGAAC CCGGCTTGAA ACGCTGCAGT AAACCAGAGT GTGATGCTTC TCCCTTGGAC 
TACGTTATTC AGGTTCAAAA CAAGCTGCTC TTGGACATAA GACGTTACAT AAAGAAATAC 
TACAGTATTC AATTGCTTTG GAGACCACAT GACAAGTCAC TTAACTCCTT GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017946959.1
CDS	50..4186
Translation	MHLVFCAPRSTVFPPYLDMSESGSFAASRSRREKTEKSGRKEALERLKRAKAGEKVKYEVEEISSIYEEVDEEQYSKIVRDRQDDDWIVDDDGTGYVEDGREIFDDDLEDNALADSGKRAKGAPKDKSNVKKSSVSKPNNIKSMFMASAVKRTTDKTVDLSKDDLLGDLLQDLKSQALPITPPPVITLKKKKLAGSPLNPFSVPPAAPKVLPVKKPPALAKLGHLSSAAPSNNSASSFVPREIKNEPNSDLISSAPLNVKTQVKEEDTSMVEFDDGDFDEPMEEDNLESPSVVSSTIKKEEPKIKCVKEDSVKMEESSFMTSAALNESCWDQIDEVESMTTEVQVDSSRLPLVTGADGSQVFKFYWLDAYEDQYSQPGVVYLFGKVWIETAEAYVSCCVSVKNIERTVYLLPRENRVQLSTGKDTGVPVSMMNVYQEFNESVAEKYKIMKFKSKKVEKDYAFEIPDVPASSEYLEVRYSADSPQLPQDLKGETFSHVFGTNTSSLELFLLSRKIKGPSWLEIKSPQLSSQPMSWCKVEAVVMRPDQVNVVKDLAPPPVVVLSLSMKTVQNAKTHQNEIVAMAALVHHKFPFDKAPPQPPFQTHFCVLSKPNDCIFPYDFKEAVKRKNANIEIALTERTLLGFFLAKIHKIDPDIIVGHDIYGFDLEVLLQRISSCKVPFWSKIGRLRRSVMPKLGGRSGFAERNAACGRIICDTEISAKELIRCKSYHLSELVHQILKTERVLMPPENIRNAYSDSAHLLYMLENTWTDAKFILQIMCELNVLPLALQITNIAGNVLSRTLMGGRSERNEYLLLHAFTENNFIVPDKPVFKKIQQTTVEDDDNMDTDQNKNKKGRKKAAYAGGLVLEPKVGFYDKFILLLDFNSLYPSIIQEYNICFTTVQREAPTSQKGEDQEEIPELPHSDLEMGILPREIRKLVERRRHVKQLMKQPDLNPDLYLQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTHQGREILLHTKEMVQKMNLEVIYGDTDSIMINTNCNNLEEVFKLGNRVKSEVNKSYKLLEIDIDGIFKSLLLLKKKKYAALTVEPTGDGKYVTKQELKGLDIVRRDWCELAKEAGNYVISQILSDQPRDNIVENIQKKLTEIGENVTNGTVPISQYEINKALTKDPQDYPDKKSLPHVHVAMWINSQGGRKVKAGDTISYVICQDGSNLSASQRAYAPEQLQKQENLSIDTQYYLSQQVHPVVARICEPIDGIDSALIAMWLGLDPSQFRAHRHHQQDEENDALLGGPSQLTDEEKYRDCERFKFFCPKCGTENIYDNVFDGSGLQIEPGLKRCSKPECDASPLDYVIQVQNKLLLDIRRYIKKYYSIQLLWRPHDKSLNSLK

Target ORF information:

RefSeq Version	XM_018091470.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis polymerase (DNA directed), alpha 1, catalytic subunit (pola1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091470.1

ORF Insert Sequence:

ATGCACTTGG TGTTCTGTGC GCCCCGCAGT ACCGTCTTCC CTCCTTACCT TGACATGTCA 
GAATCTGGAA GTTTTGCAGC ATCACGTTCG CGGCGTGAAA AAACAGAAAA GAGCGGCCGT 
AAAGAAGCAT TGGAACGTTT GAAAAGAGCA AAAGCTGGTG AAAAAGTCAA ATATGAGGTG 
GAAGAAATTT CAAGCATTTA TGAGGAGGTG GATGAAGAAC AATACTCTAA AATTGTTCGT 
GACCGACAGG ATGATGATTG GATAGTGGAT GATGATGGGA CCGGTTATGT AGAAGATGGA 
AGAGAGATTT TTGATGATGA CTTAGAAGAC AATGCTCTTG CTGATTCAGG TAAAAGAGCT 
AAGGGAGCAC CCAAAGATAA ATCTAATGTA AAAAAGTCAT CGGTGTCGAA GCCAAATAAT 
ATCAAGTCAA TGTTTATGGC CAGTGCTGTC AAAAGGACAA CAGATAAAAC TGTGGATCTG 
TCAAAAGATG ACTTGCTTGG TGATTTACTG CAAGATTTAA AGTCACAGGC TCTTCCTATA 
ACACCACCCC CAGTAATTAC TCTAAAGAAG AAGAAACTTG CAGGGTCTCC TTTAAACCCT 
TTCTCGGTAC CACCTGCTGC CCCAAAGGTT TTACCTGTGA AAAAACCACC AGCTTTAGCA 
AAGCTTGGGC ATCTCTCAAG TGCTGCGCCA TCTAACAACT CTGCCAGTTC TTTTGTACCC 
CGGGAAATTA AGAATGAGCC AAATTCTGAT TTGATTTCTT CTGCTCCTTT GAATGTGAAA 
ACTCAAGTAA AAGAAGAGGA TACGTCAATG GTTGAGTTCG ATGATGGAGA TTTTGATGAA 
CCAATGGAAG AAGACAATTT GGAATCTCCT TCAGTTGTTT CTAGCACAAT TAAGAAGGAA 
GAACCAAAAA TTAAGTGTGT GAAAGAGGAT AGTGTTAAAA TGGAGGAAAG TTCTTTCATG 
ACTTCCGCTG CTTTAAATGA ATCTTGTTGG GATCAAATTG ATGAGGTTGA ATCAATGACC 
ACTGAAGTTC AGGTAGATTC AAGCCGCCTA CCCTTGGTTA CAGGTGCAGA TGGAAGTCAG 
GTGTTTAAGT TTTATTGGCT AGATGCCTAT GAAGACCAGT ACAGTCAGCC AGGAGTAGTC 
TACTTATTTG GAAAAGTTTG GATAGAGACA GCTGAGGCGT ATGTCAGCTG CTGTGTTAGT 
GTGAAGAACA TTGAGAGGAC TGTATATCTC CTACCTCGTG AAAATAGAGT GCAGCTTTCT 
ACAGGCAAAG ACACTGGAGT ACCAGTGTCT ATGATGAATG TATACCAGGA GTTTAATGAA 
AGTGTTGCAG AAAAGTACAA AATCATGAAG TTCAAGTCTA AGAAAGTTGA GAAGGATTAT 
GCTTTTGAAA TTCCTGATGT TCCAGCCAGC TCTGAATATC TAGAGGTTCG ATATTCTGCG 
GACTCACCTC AACTTCCTCA GGATCTAAAA GGAGAAACAT TTTCACATGT GTTTGGAACA 
AATACTTCAA GCCTGGAGCT TTTCTTGCTT AGCAGAAAAA TAAAAGGGCC CAGCTGGCTG 
GAGATAAAAA GCCCACAGCT GAGCAGTCAG CCCATGAGCT GGTGCAAGGT TGAGGCAGTG 
GTGATGAGGC CTGACCAAGT GAATGTTGTG AAGGATCTGG CACCTCCACC TGTTGTTGTT 
TTGTCGCTCA GTATGAAGAC TGTGCAGAAT GCAAAGACTC ACCAAAATGA GATTGTGGCA 
ATGGCTGCTT TGGTTCATCA CAAATTTCCC TTTGATAAAG CACCACCACA GCCACCATTC 
CAGACACATT TTTGTGTTCT GAGCAAACCT AATGATTGCA TTTTCCCATA CGACTTTAAG 
GAAGCGGTGA AACGAAAGAA TGCAAATATT GAGATTGCGT TAACTGAACG AACTCTACTG 
GGCTTTTTCC TAGCAAAAAT TCATAAAATT GATCCAGATA TTATTGTAGG CCATGACATT 
TATGGTTTTG ATCTCGAAGT GTTGCTTCAA AGAATTAGCT CGTGCAAGGT TCCTTTCTGG 
TCCAAAATCG GTAGACTGAG AAGATCTGTT ATGCCAAAAC TTGGGGGTCG AAGTGGCTTT 
GCAGAAAGGA ATGCAGCTTG TGGTCGCATC ATTTGCGATA CTGAGATTTC TGCCAAGGAG 
TTAATTCGAT GTAAAAGCTA TCATTTGTCA GAACTGGTAC ATCAAATATT GAAAACAGAG 
AGAGTTTTGA TGCCACCAGA AAACATCCGC AATGCATACA GTGATTCAGC ACATCTTCTT 
TACATGCTGG AGAACACCTG GACTGATGCA AAATTCATCC TTCAGATCAT GTGTGAGCTG 
AATGTTCTTC CCCTGGCCTT GCAGATCACA AATATTGCAG GCAATGTCTT GTCAAGGACA 
TTAATGGGTG GACGCTCAGA AAGAAACGAG TACCTTCTCC TTCATGCCTT CACAGAAAAT 
AACTTCATAG TGCCAGACAA GCCAGTGTTC AAGAAAATTC AACAGACCAC TGTGGAGGAC 
GATGATAATA TGGACACAGA TCAAAATAAA AATAAGAAAG GTCGAAAGAA GGCTGCCTAT 
GCTGGAGGAT TGGTCCTGGA ACCCAAAGTT GGTTTCTACG ACAAATTCAT CTTGCTTCTG 
GATTTCAACA GCTTGTACCC CTCTATCATT CAGGAATATA ATATCTGCTT TACAACAGTG 
CAAAGAGAGG CTCCTACTTC ACAGAAAGGT GAAGATCAGG AAGAAATTCC AGAGCTACCT 
CATTCTGATC TTGAAATGGG GATCTTACCA CGAGAGATAC GGAAACTGGT GGAGAGGAGG 
CGTCACGTGA AGCAGTTAAT GAAGCAGCCT GACCTAAACC CTGATTTATA CTTACAGTAT 
GACATCAGAC AAAAAGCTTT AAAGCTCACG GCAAACAGCA TGTATGGATG CCTGGGCTTC 
TCCTACAGCA GGTTCTATGC CAAACCTTTG GCTGCATTGG TTACTCACCA AGGGAGAGAG 
ATTTTGCTGC ATACCAAGGA AATGGTACAG AAGATGAATC TGGAAGTCAT ATATGGAGAC 
ACGGATTCTA TCATGATAAA CACCAATTGT AATAATTTAG AAGAAGTCTT CAAGCTTGGA 
AACCGGGTGA AAAGTGAGGT AAACAAATCA TACAAACTTT TAGAGATTGA CATTGATGGT 
ATCTTTAAAT CACTCTTGCT ACTTAAGAAA AAGAAGTATG CCGCTCTGAC TGTGGAGCCT 
ACAGGTGATG GAAAGTATGT TACTAAGCAG GAGCTTAAAG GTTTGGATAT TGTCAGAAGA 
GACTGGTGTG AACTTGCTAA AGAAGCTGGA AATTATGTTA TCAGTCAGAT TCTGTCTGAT 
CAGCCACGTG ACAATATTGT TGAGAACATT CAGAAGAAAT TAACTGAAAT TGGGGAGAAT 
GTAACAAATG GAACTGTGCC CATTAGCCAG TATGAAATAA ACAAGGCATT GACAAAGGAT 
CCTCAGGATT ACCCTGATAA AAAAAGCTTA CCTCACGTAC ATGTTGCCAT GTGGATAAAT 
TCCCAAGGTG GAAGAAAGGT GAAAGCTGGT GATACAATAT CCTATGTCAT TTGCCAGGAC 
GGATCAAATC TTAGTGCCAG CCAGAGAGCA TATGCTCCAG AGCAGCTGCA AAAACAAGAG 
AACCTTTCCA TTGACACACA ATACTACCTG TCACAGCAAG TCCACCCAGT AGTGGCTCGG 
ATCTGTGAGC CCATTGATGG AATTGATTCT GCACTCATTG CAATGTGGTT AGGACTTGAC 
CCCTCCCAGT TCCGGGCTCA CCGCCACCAT CAGCAGGATG AGGAAAATGA TGCATTGTTG 
GGAGGTCCTT CCCAACTCAC AGATGAGGAA AAATACAGAG ATTGTGAACG ATTCAAATTC 
TTCTGCCCAA AGTGTGGAAC AGAGAACATT TATGACAATG TTTTTGATGG CTCTGGGCTG 
CAGATTGAAC CCGGCTTGAA ACGCTGCAGT AAACCAGAGT GTGATGCTTC TCCCTTGGAC 
TACGTTATTC AGGTTCAAAA CAAGCTGCTC TTGGACATAA GACGTTACAT AAAGAAATAC 
TACAGTATTC AATTGCTTTG GAGACCACAT GACAAGTCAC TTAACTCCTT GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pola1 ( XM_031896702.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031752562.1 Xenopus tropicalis polymerase (DNA directed), alpha 1, catalytic subunit (pola1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa52095
Clone ID Related Accession (Same CDS sequence)	XM_031896702.1
Accession Version	XM_031896702.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA polymerase alpha catalytic subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTTGG TGTTCTGTGC GCCCCGCAGT ACCGTCTTCC CTCCTTACCT TGACATGTCA 
GAATCTGGAA GTTTTGCAGC ATCACGTTCG CGGCGTGAAA AAACAGAAAA GAGCGGCCGT 
AAAGAAGCAT TGGAACGTTT GAAAAGAGCA AAAGCTGGTG AAAAAGTCAA ATATGAGGTG 
GAAGAAATTT CAAGCATTTA TGAGGAGGTG GATGAAGAAC AATACTCTAA AATTGTTCGT 
GACCGACAGG ATGATGATTG GATAGTGGAT GATGATGGGA CCGGTTATGT AGAAGATGGA 
AGAGAGATTT TTGATGATGA CTTAGAAGAC AATGCTCTTG CTGATTCAGG TAAAAGAGCT 
AAGGGAGCAC CCAAAGATAA ATCTAATGTA AAAAAGTCAT CGGTGTCGAA GCCAAATAAT 
ATCAAGTCAA TGTTTATGGC CAGTGCTGTC AAAAGGACAA CAGATAAAAC TGTGGATCTG 
TCAAAAGATG ACTTGCTTGG TGATTTACTG CAAGATTTAA AGTCACAGGC TCTTCCTATA 
ACACCACCCC CAGTAATTAC TCTAAAGAAG AAGAAACTTG CAGGGTCTCC TTTAAACCCT 
TTCTCGGTAC CACCTGCTGC CCCAAAGGTT TTACCTGTGA AAAAACCACC AGCTTTAGCA 
AAGCTTGGGC ATCTCTCAAG TGCTGCGCCA TCTAACAACT CTGCCAGTTC TTTTGTACCC 
CGGGAAATTA AGAATGAGCC AAATTCTGAT TTGATTTCTT CTGCTCCTTT GAATGTGAAA 
ACTCAAGTAA AAGAAGAGGA TACGTCAATG GTTGAGTTCG ATGATGGAGA TTTTGATGAA 
CCAATGGAAG AAGACAATTT GGAATCTCCT TCAGTTGTTT CTAGCACAAT TAAGAAGGAA 
GAACCAAAAA TTAAGTGTGT GAAAGAGGAT AGTGTTAAAA TGGAGGAAAG TTCTTTCATG 
ACTTCCGCTG CTTTAAATGA ATCTTGTTGG GATCAAATTG ATGAGGTTGA ATCAATGACC 
ACTGAAGTTC AGGTAGATTC AAGCCGCCTA CCCTTGGTTA CAGGTGCAGA TGGAAGTCAG 
GTGTTTAAGT TTTATTGGCT AGATGCCTAT GAAGACCAGT ACAGTCAGCC AGGAGTAGTC 
TACTTATTTG GAAAAGTTTG GATAGAGACA GCTGAGGCGT ATGTCAGCTG CTGTGTTAGT 
GTGAAGAACA TTGAGAGGAC TGTATATCTC CTACCTCGTG AAAATAGAGT GCAGCTTTCT 
ACAGGCAAAG ACACTGGAGT ACCAGTGTCT ATGATGAATG TATACCAGGA GTTTAATGAA 
AGTGTTGCAG AAAAGTACAA AATCATGAAG TTCAAGTCTA AGAAAGTTGA GAAGGATTAT 
GCTTTTGAAA TTCCTGATGT TCCAGCCAGC TCTGAATATC TAGAGGTTCG ATATTCTGCG 
GACTCACCTC AACTTCCTCA GGATCTAAAA GGAGAAACAT TTTCACATGT GTTTGGAACA 
AATACTTCAA GCCTGGAGCT TTTCTTGCTT AGCAGAAAAA TAAAAGGGCC CAGCTGGCTG 
GAGATAAAAA GCCCACAGCT GAGCAGTCAG CCCATGAGCT GGTGCAAGGT TGAGGCAGTG 
GTGATGAGGC CTGACCAAGT GAATGTTGTG AAGGATCTGG CACCTCCACC TGTTGTTGTT 
TTGTCGCTCA GTATGAAGAC TGTGCAGAAT GCAAAGACTC ACCAAAATGA GATTGTGGCA 
ATGGCTGCTT TGGTTCATCA CAAATTTCCC CTTGATAAAG CACCACCACA GCCACCATTC 
CAGACACATT TTTGTGTTCT GAGCAAACCT AATGATTGCA TTTTCCCATA CGACTTTAAG 
GAAGCGGTGA AACGAAAGAA TGCAAATATT GAGATTGCGT TAACTGAACG AACTCTACTG 
GGCTTTTTCC TAGCAAAAAT TCATAAAATT GATCCAGATA TTATTGTAGG CCATGACATT 
TATGGTTTTG ATCTCGAAGT GTTGCTTCAA AGAATTAGCT CGTGCAAGGT TCCTTTCTGG 
TCCAAAATCG GTAGACTGAG AAGATCTGTT ATGCCAAAAC TTGGGGGTCG AAGTGGCTTT 
GCAGAAAGGA ATGCAGCTTG TGGTCGCATC ATTTGCGATA CTGAGATTTC TGCCAAGGAG 
TTAATTCGAT GTAAAAGCTA TCATTTGTCA GAACTGGTAC ATCAAATATT GAAAACAGAG 
AGAGTTTTGA TGCCACCAGA AAACATCCGC AATGCATACA GTGATTCAGC ACATCTTCTT 
TACATGCTGG AGAACACCTG GACTGATGCA AAATTCATCC TTCAGATCAT GTGTGAGCTG 
AATGTTCTTC CCCTGGCCTT GCAGATCACA AATATTGCAG GCAATGTCTT GTCAAGGACA 
TTAATGGGTG GACGCTCAGA AAGAAACGAG TACCTTCTCC TTCATGCCTT CACAGAAAAT 
AACTTCATAG TGCCAGACAA GCCAGTGTTC AAGAAAATTC AACAGACCAC TGTGGAGGAC 
GATGATAATA TGGACACAGA TCAAAATAAA AATAAGAAAG GTCGAAAGAA GGCTGCCTAT 
GCTGGAGGAT TGGTCCTGGA ACCCAAAGTT GGTTTCTACG ACAAATTCAT CTTGCTTCTG 
GATTTCAACA GCTTGTACCC CTCTATCATT CAGGAATATA ATATCTGCTT TACAACAGTG 
CAAAGAGAGG CTCCTACTTC ACAGAAAGGT GAAGATCAGG AAGAAATTCC AGAGCTACCT 
CATTCTGATC TTGAAATGGG GATCTTACCA CGAGAGATAC GGAAACTGGT GGAGAGGAGG 
CGTCACGTGA AGCAGTTAAT GAAGCAGCCT GACCTAAACC CTGATTTATA CTTACAGTAT 
GACATCAGAC AAAAAGCTTT AAAGCTCACG GCAAACAGCA TGTATGGATG CCTGGGCTTC 
TCCTACAGCA GGTTCTATGC CAAACCTTTG GCTGCATTGG TTACTCACCA AGGGAGAGAG 
ATTTTGCTGC ATACCAAGGA AATGGTACAG AAGATGAATC TGGAAGTCAT ATATGGAGAC 
ACGGATTCTA TCATGATAAA CACCAATTGT AATAATTTAG AAGAAGTCTT CAAGCTTGGA 
AACCGGGTGA AAAGTGAGGT AAACAAATCA TACAAACTTT TAGAGATTGA CATTGATGGT 
ATCTTTAAAT CACTCTTGCT ACTTAAGAAA AAGAAGTATG CCGCTCTGAC TGTGGAGCCT 
ACAGGTGATG GAAAGTATGT TACTAAGCAG GAGCTTAAAG GTTTGGATAT TGTCAGAAGA 
GACTGGTGTG AACTTGCTAA AGAAGCTGGA AATTATGTTA TCAGTCAGAT TCTGTCTGAT 
CAGCCACGTG ACAATATTGT TGAGAACATT CAGAAGAAAT TAACTGAAAT TGGGGAGAAT 
GTAACAAATG GAACTGTGCC CATTAGCCAG TATGAAATAA ACAAGGCATT GACAAAGGAT 
CCTCAGGATT ACCCTGATAA AAAAAGCTTA CCTCACGTAC ATGTTGCCAT GTGGATAAAT 
TCCCAAGGTG GAAGAAAGGT GAAAGCTGGT GATACAATAT CCTATGTCAT TTGCCAGGAC 
GGATCAAATC TTAGTGCCAG CCAGAGAGCA TATGCTCCAG AGCAGCTGCA AAAACAAGAG 
AACCTTTCCA TTGACACACA ATACTACCTG TCACAGCAAG TCCACCCAGT AGTGGCTCGG 
ATCTGTGAGC CCATTGATGG AATTGATTCT GCACTCATTG CAATGTGGTT AGGACTTGAC 
CCCTCCCAGT TCCGGGCTCA CCGCCACCAT CAGCAGGATG AGGAAAATGA TGCATTGTTG 
GGAGGTCCTT CCCAACTCAC AGATGAGGAA AAATACAGAG ATTGTGAACG ATTCAAATTC 
TTCTGCCCAA AGTGTGGAAC AGAGAACATT TATGACAATG TTTTTGATGG CTCTGGGCTG 
CAGATTGAAC CCGGCTTGAA ACGCTGCAGT AAACCAGAGT GTGATGCTTC TCCCTTGGAC 
TACGTTATTC AGGTTCAAAA CAAGCTGCTC TTGGACATAA GACGTTACAT AAAGAAATAC 
TACAGTGGTT GGCTTGTATG TGAAGAAAAA ACCTGCCAGA ACAGAACACG ACGAGTTCCT 
CTGAGCTTCT CCCGCAATGG GCCGATATGC CAAGCTTGCA CTAAAGCAAC GCTAAGATCA 
GAGTATCCGG AAAAGGCCCT GTACACTCAG CTTTGTTTCT ATAGATTCAT TTTTGACTGG 
GATTATGCAC TGGAGAAAGT TGTTTCTGAA CAAGAGAGAG TTATGGAAGA TGATCCTGGA 
TGTTTATGCA ACTTACTGGT TGGTCCTGAA ATATCAATCT TTTCTGGCGC TCTTATGATA 
AGAATGACTT TGGAAAAAAC ATTACTGACT CAAATAGTGA GCCTTAGACA TGCGCTGCAT 
TGCTTTTTCT GCACCTTTAC AAATCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031752562.1
CDS	52..4521
Translation	MHLVFCAPRSTVFPPYLDMSESGSFAASRSRREKTEKSGRKEALERLKRAKAGEKVKYEVEEISSIYEEVDEEQYSKIVRDRQDDDWIVDDDGTGYVEDGREIFDDDLEDNALADSGKRAKGAPKDKSNVKKSSVSKPNNIKSMFMASAVKRTTDKTVDLSKDDLLGDLLQDLKSQALPITPPPVITLKKKKLAGSPLNPFSVPPAAPKVLPVKKPPALAKLGHLSSAAPSNNSASSFVPREIKNEPNSDLISSAPLNVKTQVKEEDTSMVEFDDGDFDEPMEEDNLESPSVVSSTIKKEEPKIKCVKEDSVKMEESSFMTSAALNESCWDQIDEVESMTTEVQVDSSRLPLVTGADGSQVFKFYWLDAYEDQYSQPGVVYLFGKVWIETAEAYVSCCVSVKNIERTVYLLPRENRVQLSTGKDTGVPVSMMNVYQEFNESVAEKYKIMKFKSKKVEKDYAFEIPDVPASSEYLEVRYSADSPQLPQDLKGETFSHVFGTNTSSLELFLLSRKIKGPSWLEIKSPQLSSQPMSWCKVEAVVMRPDQVNVVKDLAPPPVVVLSLSMKTVQNAKTHQNEIVAMAALVHHKFPLDKAPPQPPFQTHFCVLSKPNDCIFPYDFKEAVKRKNANIEIALTERTLLGFFLAKIHKIDPDIIVGHDIYGFDLEVLLQRISSCKVPFWSKIGRLRRSVMPKLGGRSGFAERNAACGRIICDTEISAKELIRCKSYHLSELVHQILKTERVLMPPENIRNAYSDSAHLLYMLENTWTDAKFILQIMCELNVLPLALQITNIAGNVLSRTLMGGRSERNEYLLLHAFTENNFIVPDKPVFKKIQQTTVEDDDNMDTDQNKNKKGRKKAAYAGGLVLEPKVGFYDKFILLLDFNSLYPSIIQEYNICFTTVQREAPTSQKGEDQEEIPELPHSDLEMGILPREIRKLVERRRHVKQLMKQPDLNPDLYLQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTHQGREILLHTKEMVQKMNLEVIYGDTDSIMINTNCNNLEEVFKLGNRVKSEVNKSYKLLEIDIDGIFKSLLLLKKKKYAALTVEPTGDGKYVTKQELKGLDIVRRDWCELAKEAGNYVISQILSDQPRDNIVENIQKKLTEIGENVTNGTVPISQYEINKALTKDPQDYPDKKSLPHVHVAMWINSQGGRKVKAGDTISYVICQDGSNLSASQRAYAPEQLQKQENLSIDTQYYLSQQVHPVVARICEPIDGIDSALIAMWLGLDPSQFRAHRHHQQDEENDALLGGPSQLTDEEKYRDCERFKFFCPKCGTENIYDNVFDGSGLQIEPGLKRCSKPECDASPLDYVIQVQNKLLLDIRRYIKKYYSGWLVCEEKTCQNRTRRVPLSFSRNGPICQACTKATLRSEYPEKALYTQLCFYRFIFDWDYALEKVVSEQERVMEDDPGCLCNLLVGPEISIFSGALMIRMTLEKTLLTQIVSLRHALHCFFCTFTNL

Target ORF information:

RefSeq Version	XM_031896702.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis polymerase (DNA directed), alpha 1, catalytic subunit (pola1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031896702.1

ORF Insert Sequence:

ATGCACTTGG TGTTCTGTGC GCCCCGCAGT ACCGTCTTCC CTCCTTACCT TGACATGTCA 
GAATCTGGAA GTTTTGCAGC ATCACGTTCG CGGCGTGAAA AAACAGAAAA GAGCGGCCGT 
AAAGAAGCAT TGGAACGTTT GAAAAGAGCA AAAGCTGGTG AAAAAGTCAA ATATGAGGTG 
GAAGAAATTT CAAGCATTTA TGAGGAGGTG GATGAAGAAC AATACTCTAA AATTGTTCGT 
GACCGACAGG ATGATGATTG GATAGTGGAT GATGATGGGA CCGGTTATGT AGAAGATGGA 
AGAGAGATTT TTGATGATGA CTTAGAAGAC AATGCTCTTG CTGATTCAGG TAAAAGAGCT 
AAGGGAGCAC CCAAAGATAA ATCTAATGTA AAAAAGTCAT CGGTGTCGAA GCCAAATAAT 
ATCAAGTCAA TGTTTATGGC CAGTGCTGTC AAAAGGACAA CAGATAAAAC TGTGGATCTG 
TCAAAAGATG ACTTGCTTGG TGATTTACTG CAAGATTTAA AGTCACAGGC TCTTCCTATA 
ACACCACCCC CAGTAATTAC TCTAAAGAAG AAGAAACTTG CAGGGTCTCC TTTAAACCCT 
TTCTCGGTAC CACCTGCTGC CCCAAAGGTT TTACCTGTGA AAAAACCACC AGCTTTAGCA 
AAGCTTGGGC ATCTCTCAAG TGCTGCGCCA TCTAACAACT CTGCCAGTTC TTTTGTACCC 
CGGGAAATTA AGAATGAGCC AAATTCTGAT TTGATTTCTT CTGCTCCTTT GAATGTGAAA 
ACTCAAGTAA AAGAAGAGGA TACGTCAATG GTTGAGTTCG ATGATGGAGA TTTTGATGAA 
CCAATGGAAG AAGACAATTT GGAATCTCCT TCAGTTGTTT CTAGCACAAT TAAGAAGGAA 
GAACCAAAAA TTAAGTGTGT GAAAGAGGAT AGTGTTAAAA TGGAGGAAAG TTCTTTCATG 
ACTTCCGCTG CTTTAAATGA ATCTTGTTGG GATCAAATTG ATGAGGTTGA ATCAATGACC 
ACTGAAGTTC AGGTAGATTC AAGCCGCCTA CCCTTGGTTA CAGGTGCAGA TGGAAGTCAG 
GTGTTTAAGT TTTATTGGCT AGATGCCTAT GAAGACCAGT ACAGTCAGCC AGGAGTAGTC 
TACTTATTTG GAAAAGTTTG GATAGAGACA GCTGAGGCGT ATGTCAGCTG CTGTGTTAGT 
GTGAAGAACA TTGAGAGGAC TGTATATCTC CTACCTCGTG AAAATAGAGT GCAGCTTTCT 
ACAGGCAAAG ACACTGGAGT ACCAGTGTCT ATGATGAATG TATACCAGGA GTTTAATGAA 
AGTGTTGCAG AAAAGTACAA AATCATGAAG TTCAAGTCTA AGAAAGTTGA GAAGGATTAT 
GCTTTTGAAA TTCCTGATGT TCCAGCCAGC TCTGAATATC TAGAGGTTCG ATATTCTGCG 
GACTCACCTC AACTTCCTCA GGATCTAAAA GGAGAAACAT TTTCACATGT GTTTGGAACA 
AATACTTCAA GCCTGGAGCT TTTCTTGCTT AGCAGAAAAA TAAAAGGGCC CAGCTGGCTG 
GAGATAAAAA GCCCACAGCT GAGCAGTCAG CCCATGAGCT GGTGCAAGGT TGAGGCAGTG 
GTGATGAGGC CTGACCAAGT GAATGTTGTG AAGGATCTGG CACCTCCACC TGTTGTTGTT 
TTGTCGCTCA GTATGAAGAC TGTGCAGAAT GCAAAGACTC ACCAAAATGA GATTGTGGCA 
ATGGCTGCTT TGGTTCATCA CAAATTTCCC CTTGATAAAG CACCACCACA GCCACCATTC 
CAGACACATT TTTGTGTTCT GAGCAAACCT AATGATTGCA TTTTCCCATA CGACTTTAAG 
GAAGCGGTGA AACGAAAGAA TGCAAATATT GAGATTGCGT TAACTGAACG AACTCTACTG 
GGCTTTTTCC TAGCAAAAAT TCATAAAATT GATCCAGATA TTATTGTAGG CCATGACATT 
TATGGTTTTG ATCTCGAAGT GTTGCTTCAA AGAATTAGCT CGTGCAAGGT TCCTTTCTGG 
TCCAAAATCG GTAGACTGAG AAGATCTGTT ATGCCAAAAC TTGGGGGTCG AAGTGGCTTT 
GCAGAAAGGA ATGCAGCTTG TGGTCGCATC ATTTGCGATA CTGAGATTTC TGCCAAGGAG 
TTAATTCGAT GTAAAAGCTA TCATTTGTCA GAACTGGTAC ATCAAATATT GAAAACAGAG 
AGAGTTTTGA TGCCACCAGA AAACATCCGC AATGCATACA GTGATTCAGC ACATCTTCTT 
TACATGCTGG AGAACACCTG GACTGATGCA AAATTCATCC TTCAGATCAT GTGTGAGCTG 
AATGTTCTTC CCCTGGCCTT GCAGATCACA AATATTGCAG GCAATGTCTT GTCAAGGACA 
TTAATGGGTG GACGCTCAGA AAGAAACGAG TACCTTCTCC TTCATGCCTT CACAGAAAAT 
AACTTCATAG TGCCAGACAA GCCAGTGTTC AAGAAAATTC AACAGACCAC TGTGGAGGAC 
GATGATAATA TGGACACAGA TCAAAATAAA AATAAGAAAG GTCGAAAGAA GGCTGCCTAT 
GCTGGAGGAT TGGTCCTGGA ACCCAAAGTT GGTTTCTACG ACAAATTCAT CTTGCTTCTG 
GATTTCAACA GCTTGTACCC CTCTATCATT CAGGAATATA ATATCTGCTT TACAACAGTG 
CAAAGAGAGG CTCCTACTTC ACAGAAAGGT GAAGATCAGG AAGAAATTCC AGAGCTACCT 
CATTCTGATC TTGAAATGGG GATCTTACCA CGAGAGATAC GGAAACTGGT GGAGAGGAGG 
CGTCACGTGA AGCAGTTAAT GAAGCAGCCT GACCTAAACC CTGATTTATA CTTACAGTAT 
GACATCAGAC AAAAAGCTTT AAAGCTCACG GCAAACAGCA TGTATGGATG CCTGGGCTTC 
TCCTACAGCA GGTTCTATGC CAAACCTTTG GCTGCATTGG TTACTCACCA AGGGAGAGAG 
ATTTTGCTGC ATACCAAGGA AATGGTACAG AAGATGAATC TGGAAGTCAT ATATGGAGAC 
ACGGATTCTA TCATGATAAA CACCAATTGT AATAATTTAG AAGAAGTCTT CAAGCTTGGA 
AACCGGGTGA AAAGTGAGGT AAACAAATCA TACAAACTTT TAGAGATTGA CATTGATGGT 
ATCTTTAAAT CACTCTTGCT ACTTAAGAAA AAGAAGTATG CCGCTCTGAC TGTGGAGCCT 
ACAGGTGATG GAAAGTATGT TACTAAGCAG GAGCTTAAAG GTTTGGATAT TGTCAGAAGA 
GACTGGTGTG AACTTGCTAA AGAAGCTGGA AATTATGTTA TCAGTCAGAT TCTGTCTGAT 
CAGCCACGTG ACAATATTGT TGAGAACATT CAGAAGAAAT TAACTGAAAT TGGGGAGAAT 
GTAACAAATG GAACTGTGCC CATTAGCCAG TATGAAATAA ACAAGGCATT GACAAAGGAT 
CCTCAGGATT ACCCTGATAA AAAAAGCTTA CCTCACGTAC ATGTTGCCAT GTGGATAAAT 
TCCCAAGGTG GAAGAAAGGT GAAAGCTGGT GATACAATAT CCTATGTCAT TTGCCAGGAC 
GGATCAAATC TTAGTGCCAG CCAGAGAGCA TATGCTCCAG AGCAGCTGCA AAAACAAGAG 
AACCTTTCCA TTGACACACA ATACTACCTG TCACAGCAAG TCCACCCAGT AGTGGCTCGG 
ATCTGTGAGC CCATTGATGG AATTGATTCT GCACTCATTG CAATGTGGTT AGGACTTGAC 
CCCTCCCAGT TCCGGGCTCA CCGCCACCAT CAGCAGGATG AGGAAAATGA TGCATTGTTG 
GGAGGTCCTT CCCAACTCAC AGATGAGGAA AAATACAGAG ATTGTGAACG ATTCAAATTC 
TTCTGCCCAA AGTGTGGAAC AGAGAACATT TATGACAATG TTTTTGATGG CTCTGGGCTG 
CAGATTGAAC CCGGCTTGAA ACGCTGCAGT AAACCAGAGT GTGATGCTTC TCCCTTGGAC 
TACGTTATTC AGGTTCAAAA CAAGCTGCTC TTGGACATAA GACGTTACAT AAAGAAATAC 
TACAGTGGTT GGCTTGTATG TGAAGAAAAA ACCTGCCAGA ACAGAACACG ACGAGTTCCT 
CTGAGCTTCT CCCGCAATGG GCCGATATGC CAAGCTTGCA CTAAAGCAAC GCTAAGATCA 
GAGTATCCGG AAAAGGCCCT GTACACTCAG CTTTGTTTCT ATAGATTCAT TTTTGACTGG 
GATTATGCAC TGGAGAAAGT TGTTTCTGAA CAAGAGAGAG TTATGGAAGA TGATCCTGGA 
TGTTTATGCA ACTTACTGGT TGGTCCTGAA ATATCAATCT TTTCTGGCGC TCTTATGATA 
AGAATGACTT TGGAAAAAAC ATTACTGACT CAAATAGTGA GCCTTAGACA TGCGCTGCAT 
TGCTTTTTCT GCACCTTTAC AAATCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pola1 ( XM_012957632.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012813086.2 Xenopus tropicalis polymerase (DNA directed), alpha 1, catalytic subunit (pola1), transcript variant X2, mRNA.

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CloneID	OXa52096
Clone ID Related Accession (Same CDS sequence)	XM_012957632.3
Accession Version	XM_012957632.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4443bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA polymerase alpha catalytic subunit isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957632.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTTGG TGTTCTGTGC GCCCCGCAGT ACCGTCTTCC CTCCTTACCT TGACATGTCA 
GAATCTGGAA GTTTTGCAGC ATCACGTTCG CGGCGTGAAA AAACAGAAAA GAGCGGCCGT 
AAAGAAGCAT TGGAACGTTT GAAAAGAGCA AAAGCTGGTG AAAAAGTCAA ATATGAGGTG 
GAAGAAATTT CAAGCATTTA TGAGGAGGTG GATGAAGAAC AATACTCTAA AATTGTTCGT 
GACCGACAGG ATGATGATTG GATAGTGGAT GATGATGGGA CCGGTTATGT AGAAGATGGA 
AGAGAGATTT TTGATGATGA CTTAGAAGAC AATGCTCTTG CTGATTCAGG TAAAAGAGCT 
AAGGGAGCAC CCAAAGATAA ATCTAATGTA AAAAAGTCAT CGGTGTCGAA GCCAAATAAT 
ATCAAGTCAA TGTTTATGGC CAGTGCTGTC AAAAGGACAA CAGATAAAAC TGTGGATCTG 
TCAAAAGATG ACTTGCTTGG TGATTTACTG CAAGATTTAA AGTCACAGGC TCTTCCTATA 
ACACCACCCC CAGTAATTAC TCTAAAGAAG AAGAAACTTG CAGGGTCTCC TTTAAACCCT 
TTCTCGGTAC CACCTGCTGC CCCAAAGGTT TTACCTGTGA AAAAACCACC AGCTTTAGCA 
AAGCTTGGGC ATCTCTCAAG TGCTGCGCCA TCTAACAACT CTGCCAGTTC TTTTGTACCC 
CGGGAAATTA AGAATGAGCC AAATTCTGAT TTGATTTCTT CTGCTCCTTT GAATGTGAAA 
ACTCAAGTAA AAGAAGAGGA TACGTCAATG GTTGAGTTCG ATGATGGAGA TTTTGATGAA 
CCAATGGAAG AAGACAATTT GGAATCTCCT TCAGTTGTTT CTAGCACAAT TAAGAAGGAA 
GAACCAAAAA TTAAGTGTGT GAAAGAGGAT AGTGTTAAAA TGGAGGAAAG TTCTTTCATG 
ACTTCCGCTG CTTTAAATGA ATCTTGTTGG GATCAAATTG ATGAGGTTGA ATCAATGACC 
ACTGAAGTTC AGGTAGATTC AAGCCGCCTA CCCTTGGTTA CAGGTGCAGA TGGAAGTCAG 
GTGTTTAAGT TTTATTGGCT AGATGCCTAT GAAGACCAGT ACAGTCAGCC AGGAGTAGTC 
TACTTATTTG GAAAAGTTTG GATAGAGACA GCTGAGGCGT ATGTCAGCTG CTGTGTTAGT 
GTGAAGAACA TTGAGAGGAC TGTATATCTC CTACCTCGTG AAAATAGAGT GCAGCTTTCT 
ACAGGCAAAG ACACTGGAGT ACCAGTGTCT ATGATGAATG TATACCAGGA GTTTAATGAA 
AGTGTTGCAG AAAAGTACAA AATCATGAAG TTCAAGTCTA AGAAAGTTGA GAAGGATTAT 
GCTTTTGAAA TTCCTGATGT TCCAGCCAGC TCTGAATATC TAGAGGTTCG ATATTCTGCG 
GACTCACCTC AACTTCCTCA GGATCTAAAA GGAGAAACAT TTTCACATGT GTTTGGAACA 
AATACTTCAA GCCTGGAGCT TTTCTTGCTT AGCAGAAAAA TAAAAGGGCC CAGCTGGCTG 
GAGATAAAAA GCCCACAGCT GAGCAGTCAG CCCATGAGCT GGTGCAAGGT TGAGGCAGTG 
GTGATGAGGC CTGACCAAGT GAATGTTGTG AAGGATCTGG CACCTCCACC TGTTGTTGTT 
TTGTCGCTCA GTATGAAGAC TGTGCAGAAT GCAAAGACTC ACCAAAATGA GATTGTGGCA 
ATGGCTGCTT TGGTTCATCA CAAATTTCCC CTTGATAAAG CACCACCACA GCCACCATTC 
CAGACACATT TTTGTGTTCT GAGCAAACCT AATGATTGCA TTTTCCCATA CGACTTTAAG 
GAAGCGGTGA AACGAAAGAA TGCAAATATT GAGATTGCGT TAACTGAACG AACTCTACTG 
GGCTTTTTCC TAGCAAAAAT TCATAAAATT GATCCAGATA TTATTGTAGG CCATGACATT 
TATGGTTTTG ATCTCGAAGT GTTGCTTCAA AGAATTAGCT CGTGCAAGGT TCCTTTCTGG 
TCCAAAATCG GTAGACTGAG AAGATCTGTT ATGCCAAAAC TTGGGGGTCG AAGTGGCTTT 
GCAGAAAGGA ATGCAGCTTG TGGTCGCATC ATTTGCGATA CTGAGATTTC TGCCAAGGAG 
TTAATTCGAT GTAAAAGCTA TCATTTGTCA GAACTGGTAC ATCAAATATT GAAAACAGAG 
AGAGTTTTGA TGCCACCAGA AAACATCCGC AATGCATACA GTGATTCAGC ACATCTTCTT 
TACATGCTGG AGAACACCTG GACTGATGCA AAATTCATCC TTCAGATCAT GTGTGAGCTG 
AATGTTCTTC CCCTGGCCTT GCAGATCACA AATATTGCAG GCAATGTCTT GTCAAGGACA 
TTAATGGGTG GACGCTCAGA AAGAAACGAG TACCTTCTCC TTCATGCCTT CACAGAAAAT 
AACTTCATAG TGCCAGACAA GCCAGTGTTC AAGAAAATTC AACAGACCAC TGTGGAGGAC 
GATGATAATA TGGACACAGA TCAAAATAAA AATAAGAAAG GTCGAAAGAA GGCTGCCTAT 
GCTGGAGGAT TGGTCCTGGA ACCCAAAGTT GGTTTCTACG ACAAATTCAT CTTGCTTCTG 
GATTTCAACA GCTTGTACCC CTCTATCATT CAGGAATATA ATATCTGCTT TACAACAGTG 
CAAAGAGAGG CTCCTACTTC ACAGAAAGGT GAAGATCAGG AAGAAATTCC AGAGCTACCT 
CATTCTGATC TTGAAATGGG GATCTTACCA CGAGAGATAC GGAAACTGGT GGAGAGGAGG 
CGTCACGTGA AGCAGTTAAT GAAGCAGCCT GACCTAAACC CTGATTTATA CTTACAGTAT 
GACATCAGAC AAAAAGCTTT AAAGCTCACG GCAAACAGCA TGTATGGATG CCTGGGCTTC 
TCCTACAGCA GGTTCTATGC CAAACCTTTG GCTGCATTGG TTACTCACCA AGGGAGAGAG 
ATTTTGCTGC ATACCAAGGA AATGGTACAG AAGATGAATC TGGAAGTCAT ATATGGAGAC 
ACGGATTCTA TCATGATAAA CACCAATTGT AATAATTTAG AAGAAGTCTT CAAGCTTGGA 
AACCGGGTGA AAAGTGAGGT AAACAAATCA TACAAACTTT TAGAGATTGA CATTGATGGT 
ATCTTTAAAT CACTCTTGCT ACTTAAGAAA AAGAAGTATG CCGCTCTGAC TGTGGAGCCT 
ACAGGTGATG GAAAGTATGT TACTAAGCAG GAGCTTAAAG GTTTGGATAT TGTCAGAAGA 
GACTGGTGTG AACTTGCTAA AGAAGCTGGA AATTATGTTA TCAGTCAGAT TCTGTCTGAT 
CAGCCACGTG ACAATATTGT TGAGAACATT CAGAAGAAAT TAACTGAAAT TGGGGAGAAT 
GTAACAAATG GAACTGTGCC CATTAGCCAG TATGAAATAA ACAAGGCATT GACAAAGGAT 
CCTCAGGATT ACCCTGATAA AAAAAGCTTA CCTCACGTAC ATGTTGCCAT GTGGATAAAT 
TCCCAAGGTG GAAGAAAGGT GAAAGCTGGT GATACAATAT CCTATGTCAT TTGCCAGGAC 
GGATCAAATC TTAGTGCCAG CCAGAGAGCA TATGCTCCAG AGCAGCTGCA AAAACAAGAG 
AACCTTTCCA TTGACACACA ATACTACCTG TCACAGCAAG TCCACCCAGT AGTGGCTCGG 
ATCTGTGAGC CCATTGATGG AATTGATTCT GCACTCATTG CAATGTGGTT AGGACTTGAC 
CCCTCCCAGT TCCGGGCTCA CCGCCACCAT CAGCAGGATG AGGAAAATGA TGCATTGTTG 
GGAGGTCCTT CCCAACTCAC AGATGAGGAA AAATACAGAG ATTGTGAACG ATTCAAATTC 
TTCTGCCCAA AGTGTGGAAC AGAGAACATT TATGACAATG TTTTTGATGG CTCTGGGCTG 
CAGATTGAAC CCGGCTTGAA ACGCTGCAGT AAACCAGAGT GTGATGCTTC TCCCTTGGAC 
TACGTTATTC AGGTTCAAAA CAAGCTGCTC TTGGACATAA GACGTTACAT AAAGAAATAC 
TACAGTGGTT GGCTTGTATG TGAAGAAAAA ACCTGCCAGA ACAGAACACG ACGAGTTCCT 
CTGAGCTTCT CCCGCAATGG GCCGATATGC CAAGCTTGCA CTAAAGCAAC GCTAAGATCA 
GAGTATCCGG AAAAGGCCCT GTACACTCAG CTTTGTTTCT ATAGATTCAT TTTTGACTGG 
GATTATGCAC TGGAGAAAGT TGTTTCTGAA CAAGAGAGAG GTCACTTAAA AAAGAAGCAT 
TTTCAAGAGT CAGAAAATCT GTACAAGAAG TTAAAGAGCA CTGTCGACCA AGTACTTTCA 
CAGAGCGGGT ATTCAGAAGT GAACCTCAGC AAGCTGTTCC AAACACTCAG CACCATTAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012813086.2
CDS	50..4492
Translation	MHLVFCAPRSTVFPPYLDMSESGSFAASRSRREKTEKSGRKEALERLKRAKAGEKVKYEVEEISSIYEEVDEEQYSKIVRDRQDDDWIVDDDGTGYVEDGREIFDDDLEDNALADSGKRAKGAPKDKSNVKKSSVSKPNNIKSMFMASAVKRTTDKTVDLSKDDLLGDLLQDLKSQALPITPPPVITLKKKKLAGSPLNPFSVPPAAPKVLPVKKPPALAKLGHLSSAAPSNNSASSFVPREIKNEPNSDLISSAPLNVKTQVKEEDTSMVEFDDGDFDEPMEEDNLESPSVVSSTIKKEEPKIKCVKEDSVKMEESSFMTSAALNESCWDQIDEVESMTTEVQVDSSRLPLVTGADGSQVFKFYWLDAYEDQYSQPGVVYLFGKVWIETAEAYVSCCVSVKNIERTVYLLPRENRVQLSTGKDTGVPVSMMNVYQEFNESVAEKYKIMKFKSKKVEKDYAFEIPDVPASSEYLEVRYSADSPQLPQDLKGETFSHVFGTNTSSLELFLLSRKIKGPSWLEIKSPQLSSQPMSWCKVEAVVMRPDQVNVVKDLAPPPVVVLSLSMKTVQNAKTHQNEIVAMAALVHHKFPLDKAPPQPPFQTHFCVLSKPNDCIFPYDFKEAVKRKNANIEIALTERTLLGFFLAKIHKIDPDIIVGHDIYGFDLEVLLQRISSCKVPFWSKIGRLRRSVMPKLGGRSGFAERNAACGRIICDTEISAKELIRCKSYHLSELVHQILKTERVLMPPENIRNAYSDSAHLLYMLENTWTDAKFILQIMCELNVLPLALQITNIAGNVLSRTLMGGRSERNEYLLLHAFTENNFIVPDKPVFKKIQQTTVEDDDNMDTDQNKNKKGRKKAAYAGGLVLEPKVGFYDKFILLLDFNSLYPSIIQEYNICFTTVQREAPTSQKGEDQEEIPELPHSDLEMGILPREIRKLVERRRHVKQLMKQPDLNPDLYLQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTHQGREILLHTKEMVQKMNLEVIYGDTDSIMINTNCNNLEEVFKLGNRVKSEVNKSYKLLEIDIDGIFKSLLLLKKKKYAALTVEPTGDGKYVTKQELKGLDIVRRDWCELAKEAGNYVISQILSDQPRDNIVENIQKKLTEIGENVTNGTVPISQYEINKALTKDPQDYPDKKSLPHVHVAMWINSQGGRKVKAGDTISYVICQDGSNLSASQRAYAPEQLQKQENLSIDTQYYLSQQVHPVVARICEPIDGIDSALIAMWLGLDPSQFRAHRHHQQDEENDALLGGPSQLTDEEKYRDCERFKFFCPKCGTENIYDNVFDGSGLQIEPGLKRCSKPECDASPLDYVIQVQNKLLLDIRRYIKKYYSGWLVCEEKTCQNRTRRVPLSFSRNGPICQACTKATLRSEYPEKALYTQLCFYRFIFDWDYALEKVVSEQERGHLKKKHFQESENLYKKLKSTVDQVLSQSGYSEVNLSKLFQTLSTIK

Target ORF information:

RefSeq Version	XM_012957632.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis polymerase (DNA directed), alpha 1, catalytic subunit (pola1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957632.3

ORF Insert Sequence:

ATGCACTTGG TGTTCTGTGC GCCCCGCAGT ACCGTCTTCC CTCCTTACCT TGACATGTCA 
GAATCTGGAA GTTTTGCAGC ATCACGTTCG CGGCGTGAAA AAACAGAAAA GAGCGGCCGT 
AAAGAAGCAT TGGAACGTTT GAAAAGAGCA AAAGCTGGTG AAAAAGTCAA ATATGAGGTG 
GAAGAAATTT CAAGCATTTA TGAGGAGGTG GATGAAGAAC AATACTCTAA AATTGTTCGT 
GACCGACAGG ATGATGATTG GATAGTGGAT GATGATGGGA CCGGTTATGT AGAAGATGGA 
AGAGAGATTT TTGATGATGA CTTAGAAGAC AATGCTCTTG CTGATTCAGG TAAAAGAGCT 
AAGGGAGCAC CCAAAGATAA ATCTAATGTA AAAAAGTCAT CGGTGTCGAA GCCAAATAAT 
ATCAAGTCAA TGTTTATGGC CAGTGCTGTC AAAAGGACAA CAGATAAAAC TGTGGATCTG 
TCAAAAGATG ACTTGCTTGG TGATTTACTG CAAGATTTAA AGTCACAGGC TCTTCCTATA 
ACACCACCCC CAGTAATTAC TCTAAAGAAG AAGAAACTTG CAGGGTCTCC TTTAAACCCT 
TTCTCGGTAC CACCTGCTGC CCCAAAGGTT TTACCTGTGA AAAAACCACC AGCTTTAGCA 
AAGCTTGGGC ATCTCTCAAG TGCTGCGCCA TCTAACAACT CTGCCAGTTC TTTTGTACCC 
CGGGAAATTA AGAATGAGCC AAATTCTGAT TTGATTTCTT CTGCTCCTTT GAATGTGAAA 
ACTCAAGTAA AAGAAGAGGA TACGTCAATG GTTGAGTTCG ATGATGGAGA TTTTGATGAA 
CCAATGGAAG AAGACAATTT GGAATCTCCT TCAGTTGTTT CTAGCACAAT TAAGAAGGAA 
GAACCAAAAA TTAAGTGTGT GAAAGAGGAT AGTGTTAAAA TGGAGGAAAG TTCTTTCATG 
ACTTCCGCTG CTTTAAATGA ATCTTGTTGG GATCAAATTG ATGAGGTTGA ATCAATGACC 
ACTGAAGTTC AGGTAGATTC AAGCCGCCTA CCCTTGGTTA CAGGTGCAGA TGGAAGTCAG 
GTGTTTAAGT TTTATTGGCT AGATGCCTAT GAAGACCAGT ACAGTCAGCC AGGAGTAGTC 
TACTTATTTG GAAAAGTTTG GATAGAGACA GCTGAGGCGT ATGTCAGCTG CTGTGTTAGT 
GTGAAGAACA TTGAGAGGAC TGTATATCTC CTACCTCGTG AAAATAGAGT GCAGCTTTCT 
ACAGGCAAAG ACACTGGAGT ACCAGTGTCT ATGATGAATG TATACCAGGA GTTTAATGAA 
AGTGTTGCAG AAAAGTACAA AATCATGAAG TTCAAGTCTA AGAAAGTTGA GAAGGATTAT 
GCTTTTGAAA TTCCTGATGT TCCAGCCAGC TCTGAATATC TAGAGGTTCG ATATTCTGCG 
GACTCACCTC AACTTCCTCA GGATCTAAAA GGAGAAACAT TTTCACATGT GTTTGGAACA 
AATACTTCAA GCCTGGAGCT TTTCTTGCTT AGCAGAAAAA TAAAAGGGCC CAGCTGGCTG 
GAGATAAAAA GCCCACAGCT GAGCAGTCAG CCCATGAGCT GGTGCAAGGT TGAGGCAGTG 
GTGATGAGGC CTGACCAAGT GAATGTTGTG AAGGATCTGG CACCTCCACC TGTTGTTGTT 
TTGTCGCTCA GTATGAAGAC TGTGCAGAAT GCAAAGACTC ACCAAAATGA GATTGTGGCA 
ATGGCTGCTT TGGTTCATCA CAAATTTCCC CTTGATAAAG CACCACCACA GCCACCATTC 
CAGACACATT TTTGTGTTCT GAGCAAACCT AATGATTGCA TTTTCCCATA CGACTTTAAG 
GAAGCGGTGA AACGAAAGAA TGCAAATATT GAGATTGCGT TAACTGAACG AACTCTACTG 
GGCTTTTTCC TAGCAAAAAT TCATAAAATT GATCCAGATA TTATTGTAGG CCATGACATT 
TATGGTTTTG ATCTCGAAGT GTTGCTTCAA AGAATTAGCT CGTGCAAGGT TCCTTTCTGG 
TCCAAAATCG GTAGACTGAG AAGATCTGTT ATGCCAAAAC TTGGGGGTCG AAGTGGCTTT 
GCAGAAAGGA ATGCAGCTTG TGGTCGCATC ATTTGCGATA CTGAGATTTC TGCCAAGGAG 
TTAATTCGAT GTAAAAGCTA TCATTTGTCA GAACTGGTAC ATCAAATATT GAAAACAGAG 
AGAGTTTTGA TGCCACCAGA AAACATCCGC AATGCATACA GTGATTCAGC ACATCTTCTT 
TACATGCTGG AGAACACCTG GACTGATGCA AAATTCATCC TTCAGATCAT GTGTGAGCTG 
AATGTTCTTC CCCTGGCCTT GCAGATCACA AATATTGCAG GCAATGTCTT GTCAAGGACA 
TTAATGGGTG GACGCTCAGA AAGAAACGAG TACCTTCTCC TTCATGCCTT CACAGAAAAT 
AACTTCATAG TGCCAGACAA GCCAGTGTTC AAGAAAATTC AACAGACCAC TGTGGAGGAC 
GATGATAATA TGGACACAGA TCAAAATAAA AATAAGAAAG GTCGAAAGAA GGCTGCCTAT 
GCTGGAGGAT TGGTCCTGGA ACCCAAAGTT GGTTTCTACG ACAAATTCAT CTTGCTTCTG 
GATTTCAACA GCTTGTACCC CTCTATCATT CAGGAATATA ATATCTGCTT TACAACAGTG 
CAAAGAGAGG CTCCTACTTC ACAGAAAGGT GAAGATCAGG AAGAAATTCC AGAGCTACCT 
CATTCTGATC TTGAAATGGG GATCTTACCA CGAGAGATAC GGAAACTGGT GGAGAGGAGG 
CGTCACGTGA AGCAGTTAAT GAAGCAGCCT GACCTAAACC CTGATTTATA CTTACAGTAT 
GACATCAGAC AAAAAGCTTT AAAGCTCACG GCAAACAGCA TGTATGGATG CCTGGGCTTC 
TCCTACAGCA GGTTCTATGC CAAACCTTTG GCTGCATTGG TTACTCACCA AGGGAGAGAG 
ATTTTGCTGC ATACCAAGGA AATGGTACAG AAGATGAATC TGGAAGTCAT ATATGGAGAC 
ACGGATTCTA TCATGATAAA CACCAATTGT AATAATTTAG AAGAAGTCTT CAAGCTTGGA 
AACCGGGTGA AAAGTGAGGT AAACAAATCA TACAAACTTT TAGAGATTGA CATTGATGGT 
ATCTTTAAAT CACTCTTGCT ACTTAAGAAA AAGAAGTATG CCGCTCTGAC TGTGGAGCCT 
ACAGGTGATG GAAAGTATGT TACTAAGCAG GAGCTTAAAG GTTTGGATAT TGTCAGAAGA 
GACTGGTGTG AACTTGCTAA AGAAGCTGGA AATTATGTTA TCAGTCAGAT TCTGTCTGAT 
CAGCCACGTG ACAATATTGT TGAGAACATT CAGAAGAAAT TAACTGAAAT TGGGGAGAAT 
GTAACAAATG GAACTGTGCC CATTAGCCAG TATGAAATAA ACAAGGCATT GACAAAGGAT 
CCTCAGGATT ACCCTGATAA AAAAAGCTTA CCTCACGTAC ATGTTGCCAT GTGGATAAAT 
TCCCAAGGTG GAAGAAAGGT GAAAGCTGGT GATACAATAT CCTATGTCAT TTGCCAGGAC 
GGATCAAATC TTAGTGCCAG CCAGAGAGCA TATGCTCCAG AGCAGCTGCA AAAACAAGAG 
AACCTTTCCA TTGACACACA ATACTACCTG TCACAGCAAG TCCACCCAGT AGTGGCTCGG 
ATCTGTGAGC CCATTGATGG AATTGATTCT GCACTCATTG CAATGTGGTT AGGACTTGAC 
CCCTCCCAGT TCCGGGCTCA CCGCCACCAT CAGCAGGATG AGGAAAATGA TGCATTGTTG 
GGAGGTCCTT CCCAACTCAC AGATGAGGAA AAATACAGAG ATTGTGAACG ATTCAAATTC 
TTCTGCCCAA AGTGTGGAAC AGAGAACATT TATGACAATG TTTTTGATGG CTCTGGGCTG 
CAGATTGAAC CCGGCTTGAA ACGCTGCAGT AAACCAGAGT GTGATGCTTC TCCCTTGGAC 
TACGTTATTC AGGTTCAAAA CAAGCTGCTC TTGGACATAA GACGTTACAT AAAGAAATAC 
TACAGTGGTT GGCTTGTATG TGAAGAAAAA ACCTGCCAGA ACAGAACACG ACGAGTTCCT 
CTGAGCTTCT CCCGCAATGG GCCGATATGC CAAGCTTGCA CTAAAGCAAC GCTAAGATCA 
GAGTATCCGG AAAAGGCCCT GTACACTCAG CTTTGTTTCT ATAGATTCAT TTTTGACTGG 
GATTATGCAC TGGAGAAAGT TGTTTCTGAA CAAGAGAGAG GTCACTTAAA AAAGAAGCAT 
TTTCAAGAGT CAGAAAATCT GTACAAGAAG TTAAAGAGCA CTGTCGACCA AGTACTTTCA 
CAGAGCGGGT ATTCAGAAGT GAACCTCAGC AAGCTGTTCC AAACACTCAG CACCATTAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pola1 ( XM_031896703.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031752563.1 Xenopus tropicalis polymerase (DNA directed), alpha 1, catalytic subunit (pola1), transcript variant X3, mRNA.

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CloneID	OXa52097
Clone ID Related Accession (Same CDS sequence)	XM_031896703.1 , XM_031896704.1
Accession Version	XM_031896703.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA polymerase alpha catalytic subunit isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAAT CTGGAAGTTT TGCAGCATCA CGTTCGCGGC GTGAAAAAAC AGAAAAGAGC 
GGCCGTAAAG AAGCATTGGA ACGTTTGAAA AGAGCAAAAG CTGGTGAAAA AGTCAAATAT 
GAGGTGGAAG AAATTTCAAG CATTTATGAG GAGGTGGATG AAGAACAATA CTCTAAAATT 
GTTCGTGACC GACAGGATGA TGATTGGATA GTGGATGATG ATGGGACCGG TTATGTAGAA 
GATGGAAGAG AGATTTTTGA TGATGACTTA GAAGACAATG CTCTTGCTGA TTCAGGTAAA 
AGAGCTAAGG GAGCACCCAA AGATAAATCT AATGTAAAAA AGTCATCGGT GTCGAAGCCA 
AATAATATCA AGTCAATGTT TATGGCCAGT GCTGTCAAAA GGACAACAGA TAAAACTGTG 
GATCTGTCAA AAGATGACTT GCTTGGTGAT TTACTGCAAG ATTTAAAGTC ACAGGCTCTT 
CCTATAACAC CACCCCCAGT AATTACTCTA AAGAAGAAGA AACTTGCAGG GTCTCCTTTA 
AACCCTTTCT CGGTACCACC TGCTGCCCCA AAGGTTTTAC CTGTGAAAAA ACCACCAGCT 
TTAGCAAAGC TTGGGCATCT CTCAAGTGCT GCGCCATCTA ACAACTCTGC CAGTTCTTTT 
GTACCCCGGG AAATTAAGAA TGAGCCAAAT TCTGATTTGA TTTCTTCTGC TCCTTTGAAT 
GTGAAAACTC AAGTAAAAGA AGAGGATACG TCAATGGTTG AGTTCGATGA TGGAGATTTT 
GATGAACCAA TGGAAGAAGA CAATTTGGAA TCTCCTTCAG TTGTTTCTAG CACAATTAAG 
AAGGAAGAAC CAAAAATTAA GTGTGTGAAA GAGGATAGTG TTAAAATGGA GGAAAGTTCT 
TTCATGACTT CCGCTGCTTT AAATGAATCT TGTTGGGATC AAATTGATGA GGTTGAATCA 
ATGACCACTG AAGTTCAGGT AGATTCAAGC CGCCTACCCT TGGTTACAGG TGCAGATGGA 
AGTCAGGTGT TTAAGTTTTA TTGGCTAGAT GCCTATGAAG ACCAGTACAG TCAGCCAGGA 
GTAGTCTACT TATTTGGAAA AGTTTGGATA GAGACAGCTG AGGCGTATGT CAGCTGCTGT 
GTTAGTGTGA AGAACATTGA GAGGACTGTA TATCTCCTAC CTCGTGAAAA TAGAGTGCAG 
CTTTCTACAG GCAAAGACAC TGGAGTACCA GTGTCTATGA TGAATGTATA CCAGGAGTTT 
AATGAAAGTG TTGCAGAAAA GTACAAAATC ATGAAGTTCA AGTCTAAGAA AGTTGAGAAG 
GATTATGCTT TTGAAATTCC TGATGTTCCA GCCAGCTCTG AATATCTAGA GGTTCGATAT 
TCTGCGGACT CACCTCAACT TCCTCAGGAT CTAAAAGGAG AAACATTTTC ACATGTGTTT 
GGAACAAATA CTTCAAGCCT GGAGCTTTTC TTGCTTAGCA GAAAAATAAA AGGGCCCAGC 
TGGCTGGAGA TAAAAAGCCC ACAGCTGAGC AGTCAGCCCA TGAGCTGGTG CAAGGTTGAG 
GCAGTGGTGA TGAGGCCTGA CCAAGTGAAT GTTGTGAAGG ATCTGGCACC TCCACCTGTT 
GTTGTTTTGT CGCTCAGTAT GAAGACTGTG CAGAATGCAA AGACTCACCA AAATGAGATT 
GTGGCAATGG CTGCTTTGGT TCATCACAAA TTTCCCCTTG ATAAAGCACC ACCACAGCCA 
CCATTCCAGA CACATTTTTG TGTTCTGAGC AAACCTAATG ATTGCATTTT CCCATACGAC 
TTTAAGGAAG CGGTGAAACG AAAGAATGCA AATATTGAGA TTGCGTTAAC TGAACGAACT 
CTACTGGGCT TTTTCCTAGC AAAAATTCAT AAAATTGATC CAGATATTAT TGTAGGCCAT 
GACATTTATG GTTTTGATCT CGAAGTGTTG CTTCAAAGAA TTAGCTCGTG CAAGGTTCCT 
TTCTGGTCCA AAATCGGTAG ACTGAGAAGA TCTGTTATGC CAAAACTTGG GGGTCGAAGT 
GGCTTTGCAG AAAGGAATGC AGCTTGTGGT CGCATCATTT GCGATACTGA GATTTCTGCC 
AAGGAGTTAA TTCGATGTAA AAGCTATCAT TTGTCAGAAC TGGTACATCA AATATTGAAA 
ACAGAGAGAG TTTTGATGCC ACCAGAAAAC ATCCGCAATG CATACAGTGA TTCAGCACAT 
CTTCTTTACA TGCTGGAGAA CACCTGGACT GATGCAAAAT TCATCCTTCA GATCATGTGT 
GAGCTGAATG TTCTTCCCCT GGCCTTGCAG ATCACAAATA TTGCAGGCAA TGTCTTGTCA 
AGGACATTAA TGGGTGGACG CTCAGAAAGA AACGAGTACC TTCTCCTTCA TGCCTTCACA 
GAAAATAACT TCATAGTGCC AGACAAGCCA GTGTTCAAGA AAATTCAACA GACCACTGTG 
GAGGACGATG ATAATATGGA CACAGATCAA AATAAAAATA AGAAAGGTCG AAAGAAGGCT 
GCCTATGCTG GAGGATTGGT CCTGGAACCC AAAGTTGGTT TCTACGACAA ATTCATCTTG 
CTTCTGGATT TCAACAGCTT GTACCCCTCT ATCATTCAGG AATATAATAT CTGCTTTACA 
ACAGTGCAAA GAGAGGCTCC TACTTCACAG AAAGGTGAAG ATCAGGAAGA AATTCCAGAG 
CTACCTCATT CTGATCTTGA AATGGGGATC TTACCACGAG AGATACGGAA ACTGGTGGAG 
AGGAGGCGTC ACGTGAAGCA GTTAATGAAG CAGCCTGACC TAAACCCTGA TTTATACTTA 
CAGTATGACA TCAGACAAAA AGCTTTAAAG CTCACGGCAA ACAGCATGTA TGGATGCCTG 
GGCTTCTCCT ACAGCAGGTT CTATGCCAAA CCTTTGGCTG CATTGGTTAC TCACCAAGGG 
AGAGAGATTT TGCTGCATAC CAAGGAAATG GTACAGAAGA TGAATCTGGA AGTCATATAT 
GGAGACACGG ATTCTATCAT GATAAACACC AATTGTAATA ATTTAGAAGA AGTCTTCAAG 
CTTGGAAACC GGGTGAAAAG TGAGGTAAAC AAATCATACA AACTTTTAGA GATTGACATT 
GATGGTATCT TTAAATCACT CTTGCTACTT AAGAAAAAGA AGTATGCCGC TCTGACTGTG 
GAGCCTACAG GTGATGGAAA GTATGTTACT AAGCAGGAGC TTAAAGGTTT GGATATTGTC 
AGAAGAGACT GGTGTGAACT TGCTAAAGAA GCTGGAAATT ATGTTATCAG TCAGATTCTG 
TCTGATCAGC CACGTGACAA TATTGTTGAG AACATTCAGA AGAAATTAAC TGAAATTGGG 
GAGAATGTAA CAAATGGAAC TGTGCCCATT AGCCAGTATG AAATAAACAA GGCATTGACA 
AAGGATCCTC AGGATTACCC TGATAAAAAA AGCTTACCTC ACGTACATGT TGCCATGTGG 
ATAAATTCCC AAGGTGGAAG AAAGGTGAAA GCTGGTGATA CAATATCCTA TGTCATTTGC 
CAGGACGGAT CAAATCTTAG TGCCAGCCAG AGAGCATATG CTCCAGAGCA GCTGCAAAAA 
CAAGAGAACC TTTCCATTGA CACACAATAC TACCTGTCAC AGCAAGTCCA CCCAGTAGTG 
GCTCGGATCT GTGAGCCCAT TGATGGAATT GATTCTGCAC TCATTGCAAT GTGGTTAGGA 
CTTGACCCCT CCCAGTTCCG GGCTCACCGC CACCATCAGC AGGATGAGGA AAATGATGCA 
TTGTTGGGAG GTCCTTCCCA ACTCACAGAT GAGGAAAAAT ACAGAGATTG TGAACGATTC 
AAATTCTTCT GCCCAAAGTG TGGAACAGAG AACATTTATG ACAATGTTTT TGATGGCTCT 
GGGCTGCAGA TTGAACCCGG CTTGAAACGC TGCAGTAAAC CAGAGTGTGA TGCTTCTCCC 
TTGGACTACG TTATTCAGGT TCAAAACAAG CTGCTCTTGG ACATAAGACG TTACATAAAG 
AAATACTACA GTGGTTGGCT TGTATGTGAA GAAAAAACCT GCCAGAACAG AACACGACGA 
GTTCCTCTGA GCTTCTCCCG CAATGGGCCG ATATGCCAAG CTTGCACTAA AGCAACGCTA 
AGATCAGAGT ATCCGGAAAA GGCCCTGTAC ACTCAGCTTT GTTTCTATAG ATTCATTTTT 
GACTGGGATT ATGCACTGGA GAAAGTTGTT TCTGAACAAG AGAGAGTTAT GGAAGATGAT 
CCTGGATGTT TATGCAACTT ACTGGTTGGT CCTGAAATAT CAATCTTTTC TGGCGCTCTT 
ATGATAAGAA TGACTTTGGA AAAAACATTA CTGACTCAAA TAGTGAGCCT TAGACATGCG 
CTGCATTGCT TTTTCTGCAC CTTTACAAAT CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031752563.1
CDS	146..4561
Translation	MSESGSFAASRSRREKTEKSGRKEALERLKRAKAGEKVKYEVEEISSIYEEVDEEQYSKIVRDRQDDDWIVDDDGTGYVEDGREIFDDDLEDNALADSGKRAKGAPKDKSNVKKSSVSKPNNIKSMFMASAVKRTTDKTVDLSKDDLLGDLLQDLKSQALPITPPPVITLKKKKLAGSPLNPFSVPPAAPKVLPVKKPPALAKLGHLSSAAPSNNSASSFVPREIKNEPNSDLISSAPLNVKTQVKEEDTSMVEFDDGDFDEPMEEDNLESPSVVSSTIKKEEPKIKCVKEDSVKMEESSFMTSAALNESCWDQIDEVESMTTEVQVDSSRLPLVTGADGSQVFKFYWLDAYEDQYSQPGVVYLFGKVWIETAEAYVSCCVSVKNIERTVYLLPRENRVQLSTGKDTGVPVSMMNVYQEFNESVAEKYKIMKFKSKKVEKDYAFEIPDVPASSEYLEVRYSADSPQLPQDLKGETFSHVFGTNTSSLELFLLSRKIKGPSWLEIKSPQLSSQPMSWCKVEAVVMRPDQVNVVKDLAPPPVVVLSLSMKTVQNAKTHQNEIVAMAALVHHKFPLDKAPPQPPFQTHFCVLSKPNDCIFPYDFKEAVKRKNANIEIALTERTLLGFFLAKIHKIDPDIIVGHDIYGFDLEVLLQRISSCKVPFWSKIGRLRRSVMPKLGGRSGFAERNAACGRIICDTEISAKELIRCKSYHLSELVHQILKTERVLMPPENIRNAYSDSAHLLYMLENTWTDAKFILQIMCELNVLPLALQITNIAGNVLSRTLMGGRSERNEYLLLHAFTENNFIVPDKPVFKKIQQTTVEDDDNMDTDQNKNKKGRKKAAYAGGLVLEPKVGFYDKFILLLDFNSLYPSIIQEYNICFTTVQREAPTSQKGEDQEEIPELPHSDLEMGILPREIRKLVERRRHVKQLMKQPDLNPDLYLQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTHQGREILLHTKEMVQKMNLEVIYGDTDSIMINTNCNNLEEVFKLGNRVKSEVNKSYKLLEIDIDGIFKSLLLLKKKKYAALTVEPTGDGKYVTKQELKGLDIVRRDWCELAKEAGNYVISQILSDQPRDNIVENIQKKLTEIGENVTNGTVPISQYEINKALTKDPQDYPDKKSLPHVHVAMWINSQGGRKVKAGDTISYVICQDGSNLSASQRAYAPEQLQKQENLSIDTQYYLSQQVHPVVARICEPIDGIDSALIAMWLGLDPSQFRAHRHHQQDEENDALLGGPSQLTDEEKYRDCERFKFFCPKCGTENIYDNVFDGSGLQIEPGLKRCSKPECDASPLDYVIQVQNKLLLDIRRYIKKYYSGWLVCEEKTCQNRTRRVPLSFSRNGPICQACTKATLRSEYPEKALYTQLCFYRFIFDWDYALEKVVSEQERVMEDDPGCLCNLLVGPEISIFSGALMIRMTLEKTLLTQIVSLRHALHCFFCTFTNL

Target ORF information:

RefSeq Version	XM_031896703.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis polymerase (DNA directed), alpha 1, catalytic subunit (pola1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031896703.1

ORF Insert Sequence:

ATGTCAGAAT CTGGAAGTTT TGCAGCATCA CGTTCGCGGC GTGAAAAAAC AGAAAAGAGC 
GGCCGTAAAG AAGCATTGGA ACGTTTGAAA AGAGCAAAAG CTGGTGAAAA AGTCAAATAT 
GAGGTGGAAG AAATTTCAAG CATTTATGAG GAGGTGGATG AAGAACAATA CTCTAAAATT 
GTTCGTGACC GACAGGATGA TGATTGGATA GTGGATGATG ATGGGACCGG TTATGTAGAA 
GATGGAAGAG AGATTTTTGA TGATGACTTA GAAGACAATG CTCTTGCTGA TTCAGGTAAA 
AGAGCTAAGG GAGCACCCAA AGATAAATCT AATGTAAAAA AGTCATCGGT GTCGAAGCCA 
AATAATATCA AGTCAATGTT TATGGCCAGT GCTGTCAAAA GGACAACAGA TAAAACTGTG 
GATCTGTCAA AAGATGACTT GCTTGGTGAT TTACTGCAAG ATTTAAAGTC ACAGGCTCTT 
CCTATAACAC CACCCCCAGT AATTACTCTA AAGAAGAAGA AACTTGCAGG GTCTCCTTTA 
AACCCTTTCT CGGTACCACC TGCTGCCCCA AAGGTTTTAC CTGTGAAAAA ACCACCAGCT 
TTAGCAAAGC TTGGGCATCT CTCAAGTGCT GCGCCATCTA ACAACTCTGC CAGTTCTTTT 
GTACCCCGGG AAATTAAGAA TGAGCCAAAT TCTGATTTGA TTTCTTCTGC TCCTTTGAAT 
GTGAAAACTC AAGTAAAAGA AGAGGATACG TCAATGGTTG AGTTCGATGA TGGAGATTTT 
GATGAACCAA TGGAAGAAGA CAATTTGGAA TCTCCTTCAG TTGTTTCTAG CACAATTAAG 
AAGGAAGAAC CAAAAATTAA GTGTGTGAAA GAGGATAGTG TTAAAATGGA GGAAAGTTCT 
TTCATGACTT CCGCTGCTTT AAATGAATCT TGTTGGGATC AAATTGATGA GGTTGAATCA 
ATGACCACTG AAGTTCAGGT AGATTCAAGC CGCCTACCCT TGGTTACAGG TGCAGATGGA 
AGTCAGGTGT TTAAGTTTTA TTGGCTAGAT GCCTATGAAG ACCAGTACAG TCAGCCAGGA 
GTAGTCTACT TATTTGGAAA AGTTTGGATA GAGACAGCTG AGGCGTATGT CAGCTGCTGT 
GTTAGTGTGA AGAACATTGA GAGGACTGTA TATCTCCTAC CTCGTGAAAA TAGAGTGCAG 
CTTTCTACAG GCAAAGACAC TGGAGTACCA GTGTCTATGA TGAATGTATA CCAGGAGTTT 
AATGAAAGTG TTGCAGAAAA GTACAAAATC ATGAAGTTCA AGTCTAAGAA AGTTGAGAAG 
GATTATGCTT TTGAAATTCC TGATGTTCCA GCCAGCTCTG AATATCTAGA GGTTCGATAT 
TCTGCGGACT CACCTCAACT TCCTCAGGAT CTAAAAGGAG AAACATTTTC ACATGTGTTT 
GGAACAAATA CTTCAAGCCT GGAGCTTTTC TTGCTTAGCA GAAAAATAAA AGGGCCCAGC 
TGGCTGGAGA TAAAAAGCCC ACAGCTGAGC AGTCAGCCCA TGAGCTGGTG CAAGGTTGAG 
GCAGTGGTGA TGAGGCCTGA CCAAGTGAAT GTTGTGAAGG ATCTGGCACC TCCACCTGTT 
GTTGTTTTGT CGCTCAGTAT GAAGACTGTG CAGAATGCAA AGACTCACCA AAATGAGATT 
GTGGCAATGG CTGCTTTGGT TCATCACAAA TTTCCCCTTG ATAAAGCACC ACCACAGCCA 
CCATTCCAGA CACATTTTTG TGTTCTGAGC AAACCTAATG ATTGCATTTT CCCATACGAC 
TTTAAGGAAG CGGTGAAACG AAAGAATGCA AATATTGAGA TTGCGTTAAC TGAACGAACT 
CTACTGGGCT TTTTCCTAGC AAAAATTCAT AAAATTGATC CAGATATTAT TGTAGGCCAT 
GACATTTATG GTTTTGATCT CGAAGTGTTG CTTCAAAGAA TTAGCTCGTG CAAGGTTCCT 
TTCTGGTCCA AAATCGGTAG ACTGAGAAGA TCTGTTATGC CAAAACTTGG GGGTCGAAGT 
GGCTTTGCAG AAAGGAATGC AGCTTGTGGT CGCATCATTT GCGATACTGA GATTTCTGCC 
AAGGAGTTAA TTCGATGTAA AAGCTATCAT TTGTCAGAAC TGGTACATCA AATATTGAAA 
ACAGAGAGAG TTTTGATGCC ACCAGAAAAC ATCCGCAATG CATACAGTGA TTCAGCACAT 
CTTCTTTACA TGCTGGAGAA CACCTGGACT GATGCAAAAT TCATCCTTCA GATCATGTGT 
GAGCTGAATG TTCTTCCCCT GGCCTTGCAG ATCACAAATA TTGCAGGCAA TGTCTTGTCA 
AGGACATTAA TGGGTGGACG CTCAGAAAGA AACGAGTACC TTCTCCTTCA TGCCTTCACA 
GAAAATAACT TCATAGTGCC AGACAAGCCA GTGTTCAAGA AAATTCAACA GACCACTGTG 
GAGGACGATG ATAATATGGA CACAGATCAA AATAAAAATA AGAAAGGTCG AAAGAAGGCT 
GCCTATGCTG GAGGATTGGT CCTGGAACCC AAAGTTGGTT TCTACGACAA ATTCATCTTG 
CTTCTGGATT TCAACAGCTT GTACCCCTCT ATCATTCAGG AATATAATAT CTGCTTTACA 
ACAGTGCAAA GAGAGGCTCC TACTTCACAG AAAGGTGAAG ATCAGGAAGA AATTCCAGAG 
CTACCTCATT CTGATCTTGA AATGGGGATC TTACCACGAG AGATACGGAA ACTGGTGGAG 
AGGAGGCGTC ACGTGAAGCA GTTAATGAAG CAGCCTGACC TAAACCCTGA TTTATACTTA 
CAGTATGACA TCAGACAAAA AGCTTTAAAG CTCACGGCAA ACAGCATGTA TGGATGCCTG 
GGCTTCTCCT ACAGCAGGTT CTATGCCAAA CCTTTGGCTG CATTGGTTAC TCACCAAGGG 
AGAGAGATTT TGCTGCATAC CAAGGAAATG GTACAGAAGA TGAATCTGGA AGTCATATAT 
GGAGACACGG ATTCTATCAT GATAAACACC AATTGTAATA ATTTAGAAGA AGTCTTCAAG 
CTTGGAAACC GGGTGAAAAG TGAGGTAAAC AAATCATACA AACTTTTAGA GATTGACATT 
GATGGTATCT TTAAATCACT CTTGCTACTT AAGAAAAAGA AGTATGCCGC TCTGACTGTG 
GAGCCTACAG GTGATGGAAA GTATGTTACT AAGCAGGAGC TTAAAGGTTT GGATATTGTC 
AGAAGAGACT GGTGTGAACT TGCTAAAGAA GCTGGAAATT ATGTTATCAG TCAGATTCTG 
TCTGATCAGC CACGTGACAA TATTGTTGAG AACATTCAGA AGAAATTAAC TGAAATTGGG 
GAGAATGTAA CAAATGGAAC TGTGCCCATT AGCCAGTATG AAATAAACAA GGCATTGACA 
AAGGATCCTC AGGATTACCC TGATAAAAAA AGCTTACCTC ACGTACATGT TGCCATGTGG 
ATAAATTCCC AAGGTGGAAG AAAGGTGAAA GCTGGTGATA CAATATCCTA TGTCATTTGC 
CAGGACGGAT CAAATCTTAG TGCCAGCCAG AGAGCATATG CTCCAGAGCA GCTGCAAAAA 
CAAGAGAACC TTTCCATTGA CACACAATAC TACCTGTCAC AGCAAGTCCA CCCAGTAGTG 
GCTCGGATCT GTGAGCCCAT TGATGGAATT GATTCTGCAC TCATTGCAAT GTGGTTAGGA 
CTTGACCCCT CCCAGTTCCG GGCTCACCGC CACCATCAGC AGGATGAGGA AAATGATGCA 
TTGTTGGGAG GTCCTTCCCA ACTCACAGAT GAGGAAAAAT ACAGAGATTG TGAACGATTC 
AAATTCTTCT GCCCAAAGTG TGGAACAGAG AACATTTATG ACAATGTTTT TGATGGCTCT 
GGGCTGCAGA TTGAACCCGG CTTGAAACGC TGCAGTAAAC CAGAGTGTGA TGCTTCTCCC 
TTGGACTACG TTATTCAGGT TCAAAACAAG CTGCTCTTGG ACATAAGACG TTACATAAAG 
AAATACTACA GTGGTTGGCT TGTATGTGAA GAAAAAACCT GCCAGAACAG AACACGACGA 
GTTCCTCTGA GCTTCTCCCG CAATGGGCCG ATATGCCAAG CTTGCACTAA AGCAACGCTA 
AGATCAGAGT ATCCGGAAAA GGCCCTGTAC ACTCAGCTTT GTTTCTATAG ATTCATTTTT 
GACTGGGATT ATGCACTGGA GAAAGTTGTT TCTGAACAAG AGAGAGTTAT GGAAGATGAT 
CCTGGATGTT TATGCAACTT ACTGGTTGGT CCTGAAATAT CAATCTTTTC TGGCGCTCTT 
ATGATAAGAA TGACTTTGGA AAAAACATTA CTGACTCAAA TAGTGAGCCT TAGACATGCG 
CTGCATTGCT TTTTCTGCAC CTTTACAAAT CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pola1 ( XM_031896704.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031752564.1 Xenopus tropicalis polymerase (DNA directed), alpha 1, catalytic subunit (pola1), transcript variant X4, mRNA.

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CloneID	OXa52097
Clone ID Related Accession (Same CDS sequence)	XM_031896703.1 , XM_031896704.1
Accession Version	XM_031896704.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA polymerase alpha catalytic subunit isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAAT CTGGAAGTTT TGCAGCATCA CGTTCGCGGC GTGAAAAAAC AGAAAAGAGC 
GGCCGTAAAG AAGCATTGGA ACGTTTGAAA AGAGCAAAAG CTGGTGAAAA AGTCAAATAT 
GAGGTGGAAG AAATTTCAAG CATTTATGAG GAGGTGGATG AAGAACAATA CTCTAAAATT 
GTTCGTGACC GACAGGATGA TGATTGGATA GTGGATGATG ATGGGACCGG TTATGTAGAA 
GATGGAAGAG AGATTTTTGA TGATGACTTA GAAGACAATG CTCTTGCTGA TTCAGGTAAA 
AGAGCTAAGG GAGCACCCAA AGATAAATCT AATGTAAAAA AGTCATCGGT GTCGAAGCCA 
AATAATATCA AGTCAATGTT TATGGCCAGT GCTGTCAAAA GGACAACAGA TAAAACTGTG 
GATCTGTCAA AAGATGACTT GCTTGGTGAT TTACTGCAAG ATTTAAAGTC ACAGGCTCTT 
CCTATAACAC CACCCCCAGT AATTACTCTA AAGAAGAAGA AACTTGCAGG GTCTCCTTTA 
AACCCTTTCT CGGTACCACC TGCTGCCCCA AAGGTTTTAC CTGTGAAAAA ACCACCAGCT 
TTAGCAAAGC TTGGGCATCT CTCAAGTGCT GCGCCATCTA ACAACTCTGC CAGTTCTTTT 
GTACCCCGGG AAATTAAGAA TGAGCCAAAT TCTGATTTGA TTTCTTCTGC TCCTTTGAAT 
GTGAAAACTC AAGTAAAAGA AGAGGATACG TCAATGGTTG AGTTCGATGA TGGAGATTTT 
GATGAACCAA TGGAAGAAGA CAATTTGGAA TCTCCTTCAG TTGTTTCTAG CACAATTAAG 
AAGGAAGAAC CAAAAATTAA GTGTGTGAAA GAGGATAGTG TTAAAATGGA GGAAAGTTCT 
TTCATGACTT CCGCTGCTTT AAATGAATCT TGTTGGGATC AAATTGATGA GGTTGAATCA 
ATGACCACTG AAGTTCAGGT AGATTCAAGC CGCCTACCCT TGGTTACAGG TGCAGATGGA 
AGTCAGGTGT TTAAGTTTTA TTGGCTAGAT GCCTATGAAG ACCAGTACAG TCAGCCAGGA 
GTAGTCTACT TATTTGGAAA AGTTTGGATA GAGACAGCTG AGGCGTATGT CAGCTGCTGT 
GTTAGTGTGA AGAACATTGA GAGGACTGTA TATCTCCTAC CTCGTGAAAA TAGAGTGCAG 
CTTTCTACAG GCAAAGACAC TGGAGTACCA GTGTCTATGA TGAATGTATA CCAGGAGTTT 
AATGAAAGTG TTGCAGAAAA GTACAAAATC ATGAAGTTCA AGTCTAAGAA AGTTGAGAAG 
GATTATGCTT TTGAAATTCC TGATGTTCCA GCCAGCTCTG AATATCTAGA GGTTCGATAT 
TCTGCGGACT CACCTCAACT TCCTCAGGAT CTAAAAGGAG AAACATTTTC ACATGTGTTT 
GGAACAAATA CTTCAAGCCT GGAGCTTTTC TTGCTTAGCA GAAAAATAAA AGGGCCCAGC 
TGGCTGGAGA TAAAAAGCCC ACAGCTGAGC AGTCAGCCCA TGAGCTGGTG CAAGGTTGAG 
GCAGTGGTGA TGAGGCCTGA CCAAGTGAAT GTTGTGAAGG ATCTGGCACC TCCACCTGTT 
GTTGTTTTGT CGCTCAGTAT GAAGACTGTG CAGAATGCAA AGACTCACCA AAATGAGATT 
GTGGCAATGG CTGCTTTGGT TCATCACAAA TTTCCCCTTG ATAAAGCACC ACCACAGCCA 
CCATTCCAGA CACATTTTTG TGTTCTGAGC AAACCTAATG ATTGCATTTT CCCATACGAC 
TTTAAGGAAG CGGTGAAACG AAAGAATGCA AATATTGAGA TTGCGTTAAC TGAACGAACT 
CTACTGGGCT TTTTCCTAGC AAAAATTCAT AAAATTGATC CAGATATTAT TGTAGGCCAT 
GACATTTATG GTTTTGATCT CGAAGTGTTG CTTCAAAGAA TTAGCTCGTG CAAGGTTCCT 
TTCTGGTCCA AAATCGGTAG ACTGAGAAGA TCTGTTATGC CAAAACTTGG GGGTCGAAGT 
GGCTTTGCAG AAAGGAATGC AGCTTGTGGT CGCATCATTT GCGATACTGA GATTTCTGCC 
AAGGAGTTAA TTCGATGTAA AAGCTATCAT TTGTCAGAAC TGGTACATCA AATATTGAAA 
ACAGAGAGAG TTTTGATGCC ACCAGAAAAC ATCCGCAATG CATACAGTGA TTCAGCACAT 
CTTCTTTACA TGCTGGAGAA CACCTGGACT GATGCAAAAT TCATCCTTCA GATCATGTGT 
GAGCTGAATG TTCTTCCCCT GGCCTTGCAG ATCACAAATA TTGCAGGCAA TGTCTTGTCA 
AGGACATTAA TGGGTGGACG CTCAGAAAGA AACGAGTACC TTCTCCTTCA TGCCTTCACA 
GAAAATAACT TCATAGTGCC AGACAAGCCA GTGTTCAAGA AAATTCAACA GACCACTGTG 
GAGGACGATG ATAATATGGA CACAGATCAA AATAAAAATA AGAAAGGTCG AAAGAAGGCT 
GCCTATGCTG GAGGATTGGT CCTGGAACCC AAAGTTGGTT TCTACGACAA ATTCATCTTG 
CTTCTGGATT TCAACAGCTT GTACCCCTCT ATCATTCAGG AATATAATAT CTGCTTTACA 
ACAGTGCAAA GAGAGGCTCC TACTTCACAG AAAGGTGAAG ATCAGGAAGA AATTCCAGAG 
CTACCTCATT CTGATCTTGA AATGGGGATC TTACCACGAG AGATACGGAA ACTGGTGGAG 
AGGAGGCGTC ACGTGAAGCA GTTAATGAAG CAGCCTGACC TAAACCCTGA TTTATACTTA 
CAGTATGACA TCAGACAAAA AGCTTTAAAG CTCACGGCAA ACAGCATGTA TGGATGCCTG 
GGCTTCTCCT ACAGCAGGTT CTATGCCAAA CCTTTGGCTG CATTGGTTAC TCACCAAGGG 
AGAGAGATTT TGCTGCATAC CAAGGAAATG GTACAGAAGA TGAATCTGGA AGTCATATAT 
GGAGACACGG ATTCTATCAT GATAAACACC AATTGTAATA ATTTAGAAGA AGTCTTCAAG 
CTTGGAAACC GGGTGAAAAG TGAGGTAAAC AAATCATACA AACTTTTAGA GATTGACATT 
GATGGTATCT TTAAATCACT CTTGCTACTT AAGAAAAAGA AGTATGCCGC TCTGACTGTG 
GAGCCTACAG GTGATGGAAA GTATGTTACT AAGCAGGAGC TTAAAGGTTT GGATATTGTC 
AGAAGAGACT GGTGTGAACT TGCTAAAGAA GCTGGAAATT ATGTTATCAG TCAGATTCTG 
TCTGATCAGC CACGTGACAA TATTGTTGAG AACATTCAGA AGAAATTAAC TGAAATTGGG 
GAGAATGTAA CAAATGGAAC TGTGCCCATT AGCCAGTATG AAATAAACAA GGCATTGACA 
AAGGATCCTC AGGATTACCC TGATAAAAAA AGCTTACCTC ACGTACATGT TGCCATGTGG 
ATAAATTCCC AAGGTGGAAG AAAGGTGAAA GCTGGTGATA CAATATCCTA TGTCATTTGC 
CAGGACGGAT CAAATCTTAG TGCCAGCCAG AGAGCATATG CTCCAGAGCA GCTGCAAAAA 
CAAGAGAACC TTTCCATTGA CACACAATAC TACCTGTCAC AGCAAGTCCA CCCAGTAGTG 
GCTCGGATCT GTGAGCCCAT TGATGGAATT GATTCTGCAC TCATTGCAAT GTGGTTAGGA 
CTTGACCCCT CCCAGTTCCG GGCTCACCGC CACCATCAGC AGGATGAGGA AAATGATGCA 
TTGTTGGGAG GTCCTTCCCA ACTCACAGAT GAGGAAAAAT ACAGAGATTG TGAACGATTC 
AAATTCTTCT GCCCAAAGTG TGGAACAGAG AACATTTATG ACAATGTTTT TGATGGCTCT 
GGGCTGCAGA TTGAACCCGG CTTGAAACGC TGCAGTAAAC CAGAGTGTGA TGCTTCTCCC 
TTGGACTACG TTATTCAGGT TCAAAACAAG CTGCTCTTGG ACATAAGACG TTACATAAAG 
AAATACTACA GTGGTTGGCT TGTATGTGAA GAAAAAACCT GCCAGAACAG AACACGACGA 
GTTCCTCTGA GCTTCTCCCG CAATGGGCCG ATATGCCAAG CTTGCACTAA AGCAACGCTA 
AGATCAGAGT ATCCGGAAAA GGCCCTGTAC ACTCAGCTTT GTTTCTATAG ATTCATTTTT 
GACTGGGATT ATGCACTGGA GAAAGTTGTT TCTGAACAAG AGAGAGTTAT GGAAGATGAT 
CCTGGATGTT TATGCAACTT ACTGGTTGGT CCTGAAATAT CAATCTTTTC TGGCGCTCTT 
ATGATAAGAA TGACTTTGGA AAAAACATTA CTGACTCAAA TAGTGAGCCT TAGACATGCG 
CTGCATTGCT TTTTCTGCAC CTTTACAAAT CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031752564.1
CDS	142..4557
Translation	MSESGSFAASRSRREKTEKSGRKEALERLKRAKAGEKVKYEVEEISSIYEEVDEEQYSKIVRDRQDDDWIVDDDGTGYVEDGREIFDDDLEDNALADSGKRAKGAPKDKSNVKKSSVSKPNNIKSMFMASAVKRTTDKTVDLSKDDLLGDLLQDLKSQALPITPPPVITLKKKKLAGSPLNPFSVPPAAPKVLPVKKPPALAKLGHLSSAAPSNNSASSFVPREIKNEPNSDLISSAPLNVKTQVKEEDTSMVEFDDGDFDEPMEEDNLESPSVVSSTIKKEEPKIKCVKEDSVKMEESSFMTSAALNESCWDQIDEVESMTTEVQVDSSRLPLVTGADGSQVFKFYWLDAYEDQYSQPGVVYLFGKVWIETAEAYVSCCVSVKNIERTVYLLPRENRVQLSTGKDTGVPVSMMNVYQEFNESVAEKYKIMKFKSKKVEKDYAFEIPDVPASSEYLEVRYSADSPQLPQDLKGETFSHVFGTNTSSLELFLLSRKIKGPSWLEIKSPQLSSQPMSWCKVEAVVMRPDQVNVVKDLAPPPVVVLSLSMKTVQNAKTHQNEIVAMAALVHHKFPLDKAPPQPPFQTHFCVLSKPNDCIFPYDFKEAVKRKNANIEIALTERTLLGFFLAKIHKIDPDIIVGHDIYGFDLEVLLQRISSCKVPFWSKIGRLRRSVMPKLGGRSGFAERNAACGRIICDTEISAKELIRCKSYHLSELVHQILKTERVLMPPENIRNAYSDSAHLLYMLENTWTDAKFILQIMCELNVLPLALQITNIAGNVLSRTLMGGRSERNEYLLLHAFTENNFIVPDKPVFKKIQQTTVEDDDNMDTDQNKNKKGRKKAAYAGGLVLEPKVGFYDKFILLLDFNSLYPSIIQEYNICFTTVQREAPTSQKGEDQEEIPELPHSDLEMGILPREIRKLVERRRHVKQLMKQPDLNPDLYLQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTHQGREILLHTKEMVQKMNLEVIYGDTDSIMINTNCNNLEEVFKLGNRVKSEVNKSYKLLEIDIDGIFKSLLLLKKKKYAALTVEPTGDGKYVTKQELKGLDIVRRDWCELAKEAGNYVISQILSDQPRDNIVENIQKKLTEIGENVTNGTVPISQYEINKALTKDPQDYPDKKSLPHVHVAMWINSQGGRKVKAGDTISYVICQDGSNLSASQRAYAPEQLQKQENLSIDTQYYLSQQVHPVVARICEPIDGIDSALIAMWLGLDPSQFRAHRHHQQDEENDALLGGPSQLTDEEKYRDCERFKFFCPKCGTENIYDNVFDGSGLQIEPGLKRCSKPECDASPLDYVIQVQNKLLLDIRRYIKKYYSGWLVCEEKTCQNRTRRVPLSFSRNGPICQACTKATLRSEYPEKALYTQLCFYRFIFDWDYALEKVVSEQERVMEDDPGCLCNLLVGPEISIFSGALMIRMTLEKTLLTQIVSLRHALHCFFCTFTNL

Target ORF information:

RefSeq Version	XM_031896704.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis polymerase (DNA directed), alpha 1, catalytic subunit (pola1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031896704.1

ORF Insert Sequence:

ATGTCAGAAT CTGGAAGTTT TGCAGCATCA CGTTCGCGGC GTGAAAAAAC AGAAAAGAGC 
GGCCGTAAAG AAGCATTGGA ACGTTTGAAA AGAGCAAAAG CTGGTGAAAA AGTCAAATAT 
GAGGTGGAAG AAATTTCAAG CATTTATGAG GAGGTGGATG AAGAACAATA CTCTAAAATT 
GTTCGTGACC GACAGGATGA TGATTGGATA GTGGATGATG ATGGGACCGG TTATGTAGAA 
GATGGAAGAG AGATTTTTGA TGATGACTTA GAAGACAATG CTCTTGCTGA TTCAGGTAAA 
AGAGCTAAGG GAGCACCCAA AGATAAATCT AATGTAAAAA AGTCATCGGT GTCGAAGCCA 
AATAATATCA AGTCAATGTT TATGGCCAGT GCTGTCAAAA GGACAACAGA TAAAACTGTG 
GATCTGTCAA AAGATGACTT GCTTGGTGAT TTACTGCAAG ATTTAAAGTC ACAGGCTCTT 
CCTATAACAC CACCCCCAGT AATTACTCTA AAGAAGAAGA AACTTGCAGG GTCTCCTTTA 
AACCCTTTCT CGGTACCACC TGCTGCCCCA AAGGTTTTAC CTGTGAAAAA ACCACCAGCT 
TTAGCAAAGC TTGGGCATCT CTCAAGTGCT GCGCCATCTA ACAACTCTGC CAGTTCTTTT 
GTACCCCGGG AAATTAAGAA TGAGCCAAAT TCTGATTTGA TTTCTTCTGC TCCTTTGAAT 
GTGAAAACTC AAGTAAAAGA AGAGGATACG TCAATGGTTG AGTTCGATGA TGGAGATTTT 
GATGAACCAA TGGAAGAAGA CAATTTGGAA TCTCCTTCAG TTGTTTCTAG CACAATTAAG 
AAGGAAGAAC CAAAAATTAA GTGTGTGAAA GAGGATAGTG TTAAAATGGA GGAAAGTTCT 
TTCATGACTT CCGCTGCTTT AAATGAATCT TGTTGGGATC AAATTGATGA GGTTGAATCA 
ATGACCACTG AAGTTCAGGT AGATTCAAGC CGCCTACCCT TGGTTACAGG TGCAGATGGA 
AGTCAGGTGT TTAAGTTTTA TTGGCTAGAT GCCTATGAAG ACCAGTACAG TCAGCCAGGA 
GTAGTCTACT TATTTGGAAA AGTTTGGATA GAGACAGCTG AGGCGTATGT CAGCTGCTGT 
GTTAGTGTGA AGAACATTGA GAGGACTGTA TATCTCCTAC CTCGTGAAAA TAGAGTGCAG 
CTTTCTACAG GCAAAGACAC TGGAGTACCA GTGTCTATGA TGAATGTATA CCAGGAGTTT 
AATGAAAGTG TTGCAGAAAA GTACAAAATC ATGAAGTTCA AGTCTAAGAA AGTTGAGAAG 
GATTATGCTT TTGAAATTCC TGATGTTCCA GCCAGCTCTG AATATCTAGA GGTTCGATAT 
TCTGCGGACT CACCTCAACT TCCTCAGGAT CTAAAAGGAG AAACATTTTC ACATGTGTTT 
GGAACAAATA CTTCAAGCCT GGAGCTTTTC TTGCTTAGCA GAAAAATAAA AGGGCCCAGC 
TGGCTGGAGA TAAAAAGCCC ACAGCTGAGC AGTCAGCCCA TGAGCTGGTG CAAGGTTGAG 
GCAGTGGTGA TGAGGCCTGA CCAAGTGAAT GTTGTGAAGG ATCTGGCACC TCCACCTGTT 
GTTGTTTTGT CGCTCAGTAT GAAGACTGTG CAGAATGCAA AGACTCACCA AAATGAGATT 
GTGGCAATGG CTGCTTTGGT TCATCACAAA TTTCCCCTTG ATAAAGCACC ACCACAGCCA 
CCATTCCAGA CACATTTTTG TGTTCTGAGC AAACCTAATG ATTGCATTTT CCCATACGAC 
TTTAAGGAAG CGGTGAAACG AAAGAATGCA AATATTGAGA TTGCGTTAAC TGAACGAACT 
CTACTGGGCT TTTTCCTAGC AAAAATTCAT AAAATTGATC CAGATATTAT TGTAGGCCAT 
GACATTTATG GTTTTGATCT CGAAGTGTTG CTTCAAAGAA TTAGCTCGTG CAAGGTTCCT 
TTCTGGTCCA AAATCGGTAG ACTGAGAAGA TCTGTTATGC CAAAACTTGG GGGTCGAAGT 
GGCTTTGCAG AAAGGAATGC AGCTTGTGGT CGCATCATTT GCGATACTGA GATTTCTGCC 
AAGGAGTTAA TTCGATGTAA AAGCTATCAT TTGTCAGAAC TGGTACATCA AATATTGAAA 
ACAGAGAGAG TTTTGATGCC ACCAGAAAAC ATCCGCAATG CATACAGTGA TTCAGCACAT 
CTTCTTTACA TGCTGGAGAA CACCTGGACT GATGCAAAAT TCATCCTTCA GATCATGTGT 
GAGCTGAATG TTCTTCCCCT GGCCTTGCAG ATCACAAATA TTGCAGGCAA TGTCTTGTCA 
AGGACATTAA TGGGTGGACG CTCAGAAAGA AACGAGTACC TTCTCCTTCA TGCCTTCACA 
GAAAATAACT TCATAGTGCC AGACAAGCCA GTGTTCAAGA AAATTCAACA GACCACTGTG 
GAGGACGATG ATAATATGGA CACAGATCAA AATAAAAATA AGAAAGGTCG AAAGAAGGCT 
GCCTATGCTG GAGGATTGGT CCTGGAACCC AAAGTTGGTT TCTACGACAA ATTCATCTTG 
CTTCTGGATT TCAACAGCTT GTACCCCTCT ATCATTCAGG AATATAATAT CTGCTTTACA 
ACAGTGCAAA GAGAGGCTCC TACTTCACAG AAAGGTGAAG ATCAGGAAGA AATTCCAGAG 
CTACCTCATT CTGATCTTGA AATGGGGATC TTACCACGAG AGATACGGAA ACTGGTGGAG 
AGGAGGCGTC ACGTGAAGCA GTTAATGAAG CAGCCTGACC TAAACCCTGA TTTATACTTA 
CAGTATGACA TCAGACAAAA AGCTTTAAAG CTCACGGCAA ACAGCATGTA TGGATGCCTG 
GGCTTCTCCT ACAGCAGGTT CTATGCCAAA CCTTTGGCTG CATTGGTTAC TCACCAAGGG 
AGAGAGATTT TGCTGCATAC CAAGGAAATG GTACAGAAGA TGAATCTGGA AGTCATATAT 
GGAGACACGG ATTCTATCAT GATAAACACC AATTGTAATA ATTTAGAAGA AGTCTTCAAG 
CTTGGAAACC GGGTGAAAAG TGAGGTAAAC AAATCATACA AACTTTTAGA GATTGACATT 
GATGGTATCT TTAAATCACT CTTGCTACTT AAGAAAAAGA AGTATGCCGC TCTGACTGTG 
GAGCCTACAG GTGATGGAAA GTATGTTACT AAGCAGGAGC TTAAAGGTTT GGATATTGTC 
AGAAGAGACT GGTGTGAACT TGCTAAAGAA GCTGGAAATT ATGTTATCAG TCAGATTCTG 
TCTGATCAGC CACGTGACAA TATTGTTGAG AACATTCAGA AGAAATTAAC TGAAATTGGG 
GAGAATGTAA CAAATGGAAC TGTGCCCATT AGCCAGTATG AAATAAACAA GGCATTGACA 
AAGGATCCTC AGGATTACCC TGATAAAAAA AGCTTACCTC ACGTACATGT TGCCATGTGG 
ATAAATTCCC AAGGTGGAAG AAAGGTGAAA GCTGGTGATA CAATATCCTA TGTCATTTGC 
CAGGACGGAT CAAATCTTAG TGCCAGCCAG AGAGCATATG CTCCAGAGCA GCTGCAAAAA 
CAAGAGAACC TTTCCATTGA CACACAATAC TACCTGTCAC AGCAAGTCCA CCCAGTAGTG 
GCTCGGATCT GTGAGCCCAT TGATGGAATT GATTCTGCAC TCATTGCAAT GTGGTTAGGA 
CTTGACCCCT CCCAGTTCCG GGCTCACCGC CACCATCAGC AGGATGAGGA AAATGATGCA 
TTGTTGGGAG GTCCTTCCCA ACTCACAGAT GAGGAAAAAT ACAGAGATTG TGAACGATTC 
AAATTCTTCT GCCCAAAGTG TGGAACAGAG AACATTTATG ACAATGTTTT TGATGGCTCT 
GGGCTGCAGA TTGAACCCGG CTTGAAACGC TGCAGTAAAC CAGAGTGTGA TGCTTCTCCC 
TTGGACTACG TTATTCAGGT TCAAAACAAG CTGCTCTTGG ACATAAGACG TTACATAAAG 
AAATACTACA GTGGTTGGCT TGTATGTGAA GAAAAAACCT GCCAGAACAG AACACGACGA 
GTTCCTCTGA GCTTCTCCCG CAATGGGCCG ATATGCCAAG CTTGCACTAA AGCAACGCTA 
AGATCAGAGT ATCCGGAAAA GGCCCTGTAC ACTCAGCTTT GTTTCTATAG ATTCATTTTT 
GACTGGGATT ATGCACTGGA GAAAGTTGTT TCTGAACAAG AGAGAGTTAT GGAAGATGAT 
CCTGGATGTT TATGCAACTT ACTGGTTGGT CCTGAAATAT CAATCTTTTC TGGCGCTCTT 
ATGATAAGAA TGACTTTGGA AAAAACATTA CTGACTCAAA TAGTGAGCCT TAGACATGCG 
CTGCATTGCT TTTTCTGCAC CTTTACAAAT CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pola1 ( XM_002933612.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002933658.1 Xenopus tropicalis polymerase (DNA directed), alpha 1, catalytic subunit (pola1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa05773
Clone ID Related Accession (Same CDS sequence)	XM_002933612.4 , XM_004911754.3
Accession Version	XM_002933612.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA polymerase alpha catalytic subunit isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002933612.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAAT CTGGAAGTTT TGCAGCATCA CGTTCGCGGC GTGAAAAAAC AGAAAAGAGC 
GGCCGTAAAG AAGCATTGGA ACGTTTGAAA AGAGCAAAAG CTGGTGAAAA AGTCAAATAT 
GAGGTGGAAG AAATTTCAAG CATTTATGAG GAGGTGGATG AAGAACAATA CTCTAAAATT 
GTTCGTGACC GACAGGATGA TGATTGGATA GTGGATGATG ATGGGACCGG TTATGTAGAA 
GATGGAAGAG AGATTTTTGA TGATGACTTA GAAGACAATG CTCTTGCTGA TTCAGGTAAA 
AGAGCTAAGG GAGCACCCAA AGATAAATCT AATGTAAAAA AGTCATCGGT GTCGAAGCCA 
AATAATATCA AGTCAATGTT TATGGCCAGT GCTGTCAAAA GGACAACAGA TAAAACTGTG 
GATCTGTCAA AAGATGACTT GCTTGGTGAT TTACTGCAAG ATTTAAAGTC ACAGGCTCTT 
CCTATAACAC CACCCCCAGT AATTACTCTA AAGAAGAAGA AACTTGCAGG GTCTCCTTTA 
AACCCTTTCT CGGTACCACC TGCTGCCCCA AAGGTTTTAC CTGTGAAAAA ACCACCAGCT 
TTAGCAAAGC TTGGGCATCT CTCAAGTGCT GCGCCATCTA ACAACTCTGC CAGTTCTTTT 
GTACCCCGGG AAATTAAGAA TGAGCCAAAT TCTGATTTGA TTTCTTCTGC TCCTTTGAAT 
GTGAAAACTC AAGTAAAAGA AGAGGATACG TCAATGGTTG AGTTCGATGA TGGAGATTTT 
GATGAACCAA TGGAAGAAGA CAATTTGGAA TCTCCTTCAG TTGTTTCTAG CACAATTAAG 
AAGGAAGAAC CAAAAATTAA GTGTGTGAAA GAGGATAGTG TTAAAATGGA GGAAAGTTCT 
TTCATGACTT CCGCTGCTTT AAATGAATCT TGTTGGGATC AAATTGATGA GGTTGAATCA 
ATGACCACTG AAGTTCAGGT AGATTCAAGC CGCCTACCCT TGGTTACAGG TGCAGATGGA 
AGTCAGGTGT TTAAGTTTTA TTGGCTAGAT GCCTATGAAG ACCAGTACAG TCAGCCAGGA 
GTAGTCTACT TATTTGGAAA AGTTTGGATA GAGACAGCTG AGGCGTATGT CAGCTGCTGT 
GTTAGTGTGA AGAACATTGA GAGGACTGTA TATCTCCTAC CTCGTGAAAA TAGAGTGCAG 
CTTTCTACAG GCAAAGACAC TGGAGTACCA GTGTCTATGA TGAATGTATA CCAGGAGTTT 
AATGAAAGTG TTGCAGAAAA GTACAAAATC ATGAAGTTCA AGTCTAAGAA AGTTGAGAAG 
GATTATGCTT TTGAAATTCC TGATGTTCCA GCCAGCTCTG AATATCTAGA GGTTCGATAT 
TCTGCGGACT CACCTCAACT TCCTCAGGAT CTAAAAGGAG AAACATTTTC ACATGTGTTT 
GGAACAAATA CTTCAAGCCT GGAGCTTTTC TTGCTTAGCA GAAAAATAAA AGGGCCCAGC 
TGGCTGGAGA TAAAAAGCCC ACAGCTGAGC AGTCAGCCCA TGAGCTGGTG CAAGGTTGAG 
GCAGTGGTGA TGAGGCCTGA CCAAGTGAAT GTTGTGAAGG ATCTGGCACC TCCACCTGTT 
GTTGTTTTGT CGCTCAGTAT GAAGACTGTG CAGAATGCAA AGACTCACCA AAATGAGATT 
GTGGCAATGG CTGCTTTGGT TCATCACAAA TTTCCCTTTG ATAAAGCACC ACCACAGCCA 
CCATTCCAGA CACATTTTTG TGTTCTGAGC AAACCTAATG ATTGCATTTT CCCATACGAC 
TTTAAGGAAG CGGTGAAACG AAAGAATGCA AATATTGAGA TTGCGTTAAC TGAACGAACT 
CTACTGGGCT TTTTCCTAGC AAAAATTCAT AAAATTGATC CAGATATTAT TGTAGGCCAT 
GACATTTATG GTTTTGATCT CGAAGTGTTG CTTCAAAGAA TTAGCTCGTG CAAGGTTCCT 
TTCTGGTCCA AAATCGGTAG ACTGAGAAGA TCTGTTATGC CAAAACTTGG GGGTCGAAGT 
GGCTTTGCAG AAAGGAATGC AGCTTGTGGT CGCATCATTT GCGATACTGA GATTTCTGCC 
AAGGAGTTAA TTCGATGTAA AAGCTATCAT TTGTCAGAAC TGGTACATCA AATATTGAAA 
ACAGAGAGAG TTTTGATGCC ACCAGAAAAC ATCCGCAATG CATACAGTGA TTCAGCACAT 
CTTCTTTACA TGCTGGAGAA CACCTGGACT GATGCAAAAT TCATCCTTCA GATCATGTGT 
GAGCTGAATG TTCTTCCCCT GGCCTTGCAG ATCACAAATA TTGCAGGCAA TGTCTTGTCA 
AGGACATTAA TGGGTGGACG CTCAGAAAGA AACGAGTACC TTCTCCTTCA TGCCTTCACA 
GAAAATAACT TCATAGTGCC AGACAAGCCA GTGTTCAAGA AAATTCAACA GACCACTGTG 
GAGGACGATG ATAATATGGA CACAGATCAA AATAAAAATA AGAAAGGTCG AAAGAAGGCT 
GCCTATGCTG GAGGATTGGT CCTGGAACCC AAAGTTGGTT TCTACGACAA ATTCATCTTG 
CTTCTGGATT TCAACAGCTT GTACCCCTCT ATCATTCAGG AATATAATAT CTGCTTTACA 
ACAGTGCAAA GAGAGGCTCC TACTTCACAG AAAGGTGAAG ATCAGGAAGA AATTCCAGAG 
CTACCTCATT CTGATCTTGA AATGGGGATC TTACCACGAG AGATACGGAA ACTGGTGGAG 
AGGAGGCGTC ACGTGAAGCA GTTAATGAAG CAGCCTGACC TAAACCCTGA TTTATACTTA 
CAGTATGACA TCAGACAAAA AGCTTTAAAG CTCACGGCAA ACAGCATGTA TGGATGCCTG 
GGCTTCTCCT ACAGCAGGTT CTATGCCAAA CCTTTGGCTG CATTGGTTAC TCACCAAGGG 
AGAGAGATTT TGCTGCATAC CAAGGAAATG GTACAGAAGA TGAATCTGGA AGTCATATAT 
GGAGACACGG ATTCTATCAT GATAAACACC AATTGTAATA ATTTAGAAGA AGTCTTCAAG 
CTTGGAAACC GGGTGAAAAG TGAGGTAAAC AAATCATACA AACTTTTAGA GATTGACATT 
GATGGTATCT TTAAATCACT CTTGCTACTT AAGAAAAAGA AGTATGCCGC TCTGACTGTG 
GAGCCTACAG GTGATGGAAA GTATGTTACT AAGCAGGAGC TTAAAGGTTT GGATATTGTC 
AGAAGAGACT GGTGTGAACT TGCTAAAGAA GCTGGAAATT ATGTTATCAG TCAGATTCTG 
TCTGATCAGC CACGTGACAA TATTGTTGAG AACATTCAGA AGAAATTAAC TGAAATTGGG 
GAGAATGTAA CAAATGGAAC TGTGCCCATT AGCCAGTATG AAATAAACAA GGCATTGACA 
AAGGATCCTC AGGATTACCC TGATAAAAAA AGCTTACCTC ACGTACATGT TGCCATGTGG 
ATAAATTCCC AAGGTGGAAG AAAGGTGAAA GCTGGTGATA CAATATCCTA TGTCATTTGC 
CAGGACGGAT CAAATCTTAG TGCCAGCCAG AGAGCATATG CTCCAGAGCA GCTGCAAAAA 
CAAGAGAACC TTTCCATTGA CACACAATAC TACCTGTCAC AGCAAGTCCA CCCAGTAGTG 
GCTCGGATCT GTGAGCCCAT TGATGGAATT GATTCTGCAC TCATTGCAAT GTGGTTAGGA 
CTTGACCCCT CCCAGTTCCG GGCTCACCGC CACCATCAGC AGGATGAGGA AAATGATGCA 
TTGTTGGGAG GTCCTTCCCA ACTCACAGAT GAGGAAAAAT ACAGAGATTG TGAACGATTC 
AAATTCTTCT GCCCAAAGTG TGGAACAGAG AACATTTATG ACAATGTTTT TGATGGCTCT 
GGGCTGCAGA TTGAACCCGG CTTGAAACGC TGCAGTAAAC CAGAGTGTGA TGCTTCTCCC 
TTGGACTACG TTATTCAGGT TCAAAACAAG CTGCTCTTGG ACATAAGACG TTACATAAAG 
AAATACTACA GTGGTTGGCT TGTATGTGAA GAAAAAACCT GCCAGAACAG AACACGACGA 
GTTCCTCTGA GCTTCTCCCG CAATGGGCCG ATATGCCAAG CTTGCACTAA AGCAACGCTA 
AGATCAGAGT ATCCGGAAAA GGCCCTGTAC ACTCAGCTTT GTTTCTATAG ATTCATTTTT 
GACTGGGATT ATGCACTGGA GAAAGTTGTT TCTGAACAAG AGAGAGGTCA CTTAAAAAAG 
AAGCATTTTC AAGAGTCAGA AAATCTGTAC AAGAAGTTAA AGAGCACTGT CGACCAAGTA 
CTTTCACAGA GCGGGTATTC AGAAGTGAAC CTCAGCAAGC TGTTCCAAAC ACTCAGCACC 
ATTAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002933658.1
CDS	170..4558
Translation	MSESGSFAASRSRREKTEKSGRKEALERLKRAKAGEKVKYEVEEISSIYEEVDEEQYSKIVRDRQDDDWIVDDDGTGYVEDGREIFDDDLEDNALADSGKRAKGAPKDKSNVKKSSVSKPNNIKSMFMASAVKRTTDKTVDLSKDDLLGDLLQDLKSQALPITPPPVITLKKKKLAGSPLNPFSVPPAAPKVLPVKKPPALAKLGHLSSAAPSNNSASSFVPREIKNEPNSDLISSAPLNVKTQVKEEDTSMVEFDDGDFDEPMEEDNLESPSVVSSTIKKEEPKIKCVKEDSVKMEESSFMTSAALNESCWDQIDEVESMTTEVQVDSSRLPLVTGADGSQVFKFYWLDAYEDQYSQPGVVYLFGKVWIETAEAYVSCCVSVKNIERTVYLLPRENRVQLSTGKDTGVPVSMMNVYQEFNESVAEKYKIMKFKSKKVEKDYAFEIPDVPASSEYLEVRYSADSPQLPQDLKGETFSHVFGTNTSSLELFLLSRKIKGPSWLEIKSPQLSSQPMSWCKVEAVVMRPDQVNVVKDLAPPPVVVLSLSMKTVQNAKTHQNEIVAMAALVHHKFPFDKAPPQPPFQTHFCVLSKPNDCIFPYDFKEAVKRKNANIEIALTERTLLGFFLAKIHKIDPDIIVGHDIYGFDLEVLLQRISSCKVPFWSKIGRLRRSVMPKLGGRSGFAERNAACGRIICDTEISAKELIRCKSYHLSELVHQILKTERVLMPPENIRNAYSDSAHLLYMLENTWTDAKFILQIMCELNVLPLALQITNIAGNVLSRTLMGGRSERNEYLLLHAFTENNFIVPDKPVFKKIQQTTVEDDDNMDTDQNKNKKGRKKAAYAGGLVLEPKVGFYDKFILLLDFNSLYPSIIQEYNICFTTVQREAPTSQKGEDQEEIPELPHSDLEMGILPREIRKLVERRRHVKQLMKQPDLNPDLYLQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTHQGREILLHTKEMVQKMNLEVIYGDTDSIMINTNCNNLEEVFKLGNRVKSEVNKSYKLLEIDIDGIFKSLLLLKKKKYAALTVEPTGDGKYVTKQELKGLDIVRRDWCELAKEAGNYVISQILSDQPRDNIVENIQKKLTEIGENVTNGTVPISQYEINKALTKDPQDYPDKKSLPHVHVAMWINSQGGRKVKAGDTISYVICQDGSNLSASQRAYAPEQLQKQENLSIDTQYYLSQQVHPVVARICEPIDGIDSALIAMWLGLDPSQFRAHRHHQQDEENDALLGGPSQLTDEEKYRDCERFKFFCPKCGTENIYDNVFDGSGLQIEPGLKRCSKPECDASPLDYVIQVQNKLLLDIRRYIKKYYSGWLVCEEKTCQNRTRRVPLSFSRNGPICQACTKATLRSEYPEKALYTQLCFYRFIFDWDYALEKVVSEQERGHLKKKHFQESENLYKKLKSTVDQVLSQSGYSEVNLSKLFQTLSTIK

Target ORF information:

RefSeq Version	XM_002933612.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis polymerase (DNA directed), alpha 1, catalytic subunit (pola1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002933612.4

ORF Insert Sequence:

ATGTCAGAAT CTGGAAGTTT TGCAGCATCA CGTTCGCGGC GTGAAAAAAC AGAAAAGAGC 
GGCCGTAAAG AAGCATTGGA ACGTTTGAAA AGAGCAAAAG CTGGTGAAAA AGTCAAATAT 
GAGGTGGAAG AAATTTCAAG CATTTATGAG GAGGTGGATG AAGAACAATA CTCTAAAATT 
GTTCGTGACC GACAGGATGA TGATTGGATA GTGGATGATG ATGGGACCGG TTATGTAGAA 
GATGGAAGAG AGATTTTTGA TGATGACTTA GAAGACAATG CTCTTGCTGA TTCAGGTAAA 
AGAGCTAAGG GAGCACCCAA AGATAAATCT AATGTAAAAA AGTCATCGGT GTCGAAGCCA 
AATAATATCA AGTCAATGTT TATGGCCAGT GCTGTCAAAA GGACAACAGA TAAAACTGTG 
GATCTGTCAA AAGATGACTT GCTTGGTGAT TTACTGCAAG ATTTAAAGTC ACAGGCTCTT 
CCTATAACAC CACCCCCAGT AATTACTCTA AAGAAGAAGA AACTTGCAGG GTCTCCTTTA 
AACCCTTTCT CGGTACCACC TGCTGCCCCA AAGGTTTTAC CTGTGAAAAA ACCACCAGCT 
TTAGCAAAGC TTGGGCATCT CTCAAGTGCT GCGCCATCTA ACAACTCTGC CAGTTCTTTT 
GTACCCCGGG AAATTAAGAA TGAGCCAAAT TCTGATTTGA TTTCTTCTGC TCCTTTGAAT 
GTGAAAACTC AAGTAAAAGA AGAGGATACG TCAATGGTTG AGTTCGATGA TGGAGATTTT 
GATGAACCAA TGGAAGAAGA CAATTTGGAA TCTCCTTCAG TTGTTTCTAG CACAATTAAG 
AAGGAAGAAC CAAAAATTAA GTGTGTGAAA GAGGATAGTG TTAAAATGGA GGAAAGTTCT 
TTCATGACTT CCGCTGCTTT AAATGAATCT TGTTGGGATC AAATTGATGA GGTTGAATCA 
ATGACCACTG AAGTTCAGGT AGATTCAAGC CGCCTACCCT TGGTTACAGG TGCAGATGGA 
AGTCAGGTGT TTAAGTTTTA TTGGCTAGAT GCCTATGAAG ACCAGTACAG TCAGCCAGGA 
GTAGTCTACT TATTTGGAAA AGTTTGGATA GAGACAGCTG AGGCGTATGT CAGCTGCTGT 
GTTAGTGTGA AGAACATTGA GAGGACTGTA TATCTCCTAC CTCGTGAAAA TAGAGTGCAG 
CTTTCTACAG GCAAAGACAC TGGAGTACCA GTGTCTATGA TGAATGTATA CCAGGAGTTT 
AATGAAAGTG TTGCAGAAAA GTACAAAATC ATGAAGTTCA AGTCTAAGAA AGTTGAGAAG 
GATTATGCTT TTGAAATTCC TGATGTTCCA GCCAGCTCTG AATATCTAGA GGTTCGATAT 
TCTGCGGACT CACCTCAACT TCCTCAGGAT CTAAAAGGAG AAACATTTTC ACATGTGTTT 
GGAACAAATA CTTCAAGCCT GGAGCTTTTC TTGCTTAGCA GAAAAATAAA AGGGCCCAGC 
TGGCTGGAGA TAAAAAGCCC ACAGCTGAGC AGTCAGCCCA TGAGCTGGTG CAAGGTTGAG 
GCAGTGGTGA TGAGGCCTGA CCAAGTGAAT GTTGTGAAGG ATCTGGCACC TCCACCTGTT 
GTTGTTTTGT CGCTCAGTAT GAAGACTGTG CAGAATGCAA AGACTCACCA AAATGAGATT 
GTGGCAATGG CTGCTTTGGT TCATCACAAA TTTCCCTTTG ATAAAGCACC ACCACAGCCA 
CCATTCCAGA CACATTTTTG TGTTCTGAGC AAACCTAATG ATTGCATTTT CCCATACGAC 
TTTAAGGAAG CGGTGAAACG AAAGAATGCA AATATTGAGA TTGCGTTAAC TGAACGAACT 
CTACTGGGCT TTTTCCTAGC AAAAATTCAT AAAATTGATC CAGATATTAT TGTAGGCCAT 
GACATTTATG GTTTTGATCT CGAAGTGTTG CTTCAAAGAA TTAGCTCGTG CAAGGTTCCT 
TTCTGGTCCA AAATCGGTAG ACTGAGAAGA TCTGTTATGC CAAAACTTGG GGGTCGAAGT 
GGCTTTGCAG AAAGGAATGC AGCTTGTGGT CGCATCATTT GCGATACTGA GATTTCTGCC 
AAGGAGTTAA TTCGATGTAA AAGCTATCAT TTGTCAGAAC TGGTACATCA AATATTGAAA 
ACAGAGAGAG TTTTGATGCC ACCAGAAAAC ATCCGCAATG CATACAGTGA TTCAGCACAT 
CTTCTTTACA TGCTGGAGAA CACCTGGACT GATGCAAAAT TCATCCTTCA GATCATGTGT 
GAGCTGAATG TTCTTCCCCT GGCCTTGCAG ATCACAAATA TTGCAGGCAA TGTCTTGTCA 
AGGACATTAA TGGGTGGACG CTCAGAAAGA AACGAGTACC TTCTCCTTCA TGCCTTCACA 
GAAAATAACT TCATAGTGCC AGACAAGCCA GTGTTCAAGA AAATTCAACA GACCACTGTG 
GAGGACGATG ATAATATGGA CACAGATCAA AATAAAAATA AGAAAGGTCG AAAGAAGGCT 
GCCTATGCTG GAGGATTGGT CCTGGAACCC AAAGTTGGTT TCTACGACAA ATTCATCTTG 
CTTCTGGATT TCAACAGCTT GTACCCCTCT ATCATTCAGG AATATAATAT CTGCTTTACA 
ACAGTGCAAA GAGAGGCTCC TACTTCACAG AAAGGTGAAG ATCAGGAAGA AATTCCAGAG 
CTACCTCATT CTGATCTTGA AATGGGGATC TTACCACGAG AGATACGGAA ACTGGTGGAG 
AGGAGGCGTC ACGTGAAGCA GTTAATGAAG CAGCCTGACC TAAACCCTGA TTTATACTTA 
CAGTATGACA TCAGACAAAA AGCTTTAAAG CTCACGGCAA ACAGCATGTA TGGATGCCTG 
GGCTTCTCCT ACAGCAGGTT CTATGCCAAA CCTTTGGCTG CATTGGTTAC TCACCAAGGG 
AGAGAGATTT TGCTGCATAC CAAGGAAATG GTACAGAAGA TGAATCTGGA AGTCATATAT 
GGAGACACGG ATTCTATCAT GATAAACACC AATTGTAATA ATTTAGAAGA AGTCTTCAAG 
CTTGGAAACC GGGTGAAAAG TGAGGTAAAC AAATCATACA AACTTTTAGA GATTGACATT 
GATGGTATCT TTAAATCACT CTTGCTACTT AAGAAAAAGA AGTATGCCGC TCTGACTGTG 
GAGCCTACAG GTGATGGAAA GTATGTTACT AAGCAGGAGC TTAAAGGTTT GGATATTGTC 
AGAAGAGACT GGTGTGAACT TGCTAAAGAA GCTGGAAATT ATGTTATCAG TCAGATTCTG 
TCTGATCAGC CACGTGACAA TATTGTTGAG AACATTCAGA AGAAATTAAC TGAAATTGGG 
GAGAATGTAA CAAATGGAAC TGTGCCCATT AGCCAGTATG AAATAAACAA GGCATTGACA 
AAGGATCCTC AGGATTACCC TGATAAAAAA AGCTTACCTC ACGTACATGT TGCCATGTGG 
ATAAATTCCC AAGGTGGAAG AAAGGTGAAA GCTGGTGATA CAATATCCTA TGTCATTTGC 
CAGGACGGAT CAAATCTTAG TGCCAGCCAG AGAGCATATG CTCCAGAGCA GCTGCAAAAA 
CAAGAGAACC TTTCCATTGA CACACAATAC TACCTGTCAC AGCAAGTCCA CCCAGTAGTG 
GCTCGGATCT GTGAGCCCAT TGATGGAATT GATTCTGCAC TCATTGCAAT GTGGTTAGGA 
CTTGACCCCT CCCAGTTCCG GGCTCACCGC CACCATCAGC AGGATGAGGA AAATGATGCA 
TTGTTGGGAG GTCCTTCCCA ACTCACAGAT GAGGAAAAAT ACAGAGATTG TGAACGATTC 
AAATTCTTCT GCCCAAAGTG TGGAACAGAG AACATTTATG ACAATGTTTT TGATGGCTCT 
GGGCTGCAGA TTGAACCCGG CTTGAAACGC TGCAGTAAAC CAGAGTGTGA TGCTTCTCCC 
TTGGACTACG TTATTCAGGT TCAAAACAAG CTGCTCTTGG ACATAAGACG TTACATAAAG 
AAATACTACA GTGGTTGGCT TGTATGTGAA GAAAAAACCT GCCAGAACAG AACACGACGA 
GTTCCTCTGA GCTTCTCCCG CAATGGGCCG ATATGCCAAG CTTGCACTAA AGCAACGCTA 
AGATCAGAGT ATCCGGAAAA GGCCCTGTAC ACTCAGCTTT GTTTCTATAG ATTCATTTTT 
GACTGGGATT ATGCACTGGA GAAAGTTGTT TCTGAACAAG AGAGAGGTCA CTTAAAAAAG 
AAGCATTTTC AAGAGTCAGA AAATCTGTAC AAGAAGTTAA AGAGCACTGT CGACCAAGTA 
CTTTCACAGA GCGGGTATTC AGAAGTGAAC CTCAGCAAGC TGTTCCAAAC ACTCAGCACC 
ATTAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pola1 ( XM_004911754.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004911811.1 Xenopus tropicalis polymerase (DNA directed), alpha 1, catalytic subunit (pola1), transcript variant X3, mRNA.

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CloneID	OXa05773
Clone ID Related Accession (Same CDS sequence)	XM_002933612.4 , XM_004911754.3
Accession Version	XM_004911754.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA polymerase alpha catalytic subunit isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004911754.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAAT CTGGAAGTTT TGCAGCATCA CGTTCGCGGC GTGAAAAAAC AGAAAAGAGC 
GGCCGTAAAG AAGCATTGGA ACGTTTGAAA AGAGCAAAAG CTGGTGAAAA AGTCAAATAT 
GAGGTGGAAG AAATTTCAAG CATTTATGAG GAGGTGGATG AAGAACAATA CTCTAAAATT 
GTTCGTGACC GACAGGATGA TGATTGGATA GTGGATGATG ATGGGACCGG TTATGTAGAA 
GATGGAAGAG AGATTTTTGA TGATGACTTA GAAGACAATG CTCTTGCTGA TTCAGGTAAA 
AGAGCTAAGG GAGCACCCAA AGATAAATCT AATGTAAAAA AGTCATCGGT GTCGAAGCCA 
AATAATATCA AGTCAATGTT TATGGCCAGT GCTGTCAAAA GGACAACAGA TAAAACTGTG 
GATCTGTCAA AAGATGACTT GCTTGGTGAT TTACTGCAAG ATTTAAAGTC ACAGGCTCTT 
CCTATAACAC CACCCCCAGT AATTACTCTA AAGAAGAAGA AACTTGCAGG GTCTCCTTTA 
AACCCTTTCT CGGTACCACC TGCTGCCCCA AAGGTTTTAC CTGTGAAAAA ACCACCAGCT 
TTAGCAAAGC TTGGGCATCT CTCAAGTGCT GCGCCATCTA ACAACTCTGC CAGTTCTTTT 
GTACCCCGGG AAATTAAGAA TGAGCCAAAT TCTGATTTGA TTTCTTCTGC TCCTTTGAAT 
GTGAAAACTC AAGTAAAAGA AGAGGATACG TCAATGGTTG AGTTCGATGA TGGAGATTTT 
GATGAACCAA TGGAAGAAGA CAATTTGGAA TCTCCTTCAG TTGTTTCTAG CACAATTAAG 
AAGGAAGAAC CAAAAATTAA GTGTGTGAAA GAGGATAGTG TTAAAATGGA GGAAAGTTCT 
TTCATGACTT CCGCTGCTTT AAATGAATCT TGTTGGGATC AAATTGATGA GGTTGAATCA 
ATGACCACTG AAGTTCAGGT AGATTCAAGC CGCCTACCCT TGGTTACAGG TGCAGATGGA 
AGTCAGGTGT TTAAGTTTTA TTGGCTAGAT GCCTATGAAG ACCAGTACAG TCAGCCAGGA 
GTAGTCTACT TATTTGGAAA AGTTTGGATA GAGACAGCTG AGGCGTATGT CAGCTGCTGT 
GTTAGTGTGA AGAACATTGA GAGGACTGTA TATCTCCTAC CTCGTGAAAA TAGAGTGCAG 
CTTTCTACAG GCAAAGACAC TGGAGTACCA GTGTCTATGA TGAATGTATA CCAGGAGTTT 
AATGAAAGTG TTGCAGAAAA GTACAAAATC ATGAAGTTCA AGTCTAAGAA AGTTGAGAAG 
GATTATGCTT TTGAAATTCC TGATGTTCCA GCCAGCTCTG AATATCTAGA GGTTCGATAT 
TCTGCGGACT CACCTCAACT TCCTCAGGAT CTAAAAGGAG AAACATTTTC ACATGTGTTT 
GGAACAAATA CTTCAAGCCT GGAGCTTTTC TTGCTTAGCA GAAAAATAAA AGGGCCCAGC 
TGGCTGGAGA TAAAAAGCCC ACAGCTGAGC AGTCAGCCCA TGAGCTGGTG CAAGGTTGAG 
GCAGTGGTGA TGAGGCCTGA CCAAGTGAAT GTTGTGAAGG ATCTGGCACC TCCACCTGTT 
GTTGTTTTGT CGCTCAGTAT GAAGACTGTG CAGAATGCAA AGACTCACCA AAATGAGATT 
GTGGCAATGG CTGCTTTGGT TCATCACAAA TTTCCCTTTG ATAAAGCACC ACCACAGCCA 
CCATTCCAGA CACATTTTTG TGTTCTGAGC AAACCTAATG ATTGCATTTT CCCATACGAC 
TTTAAGGAAG CGGTGAAACG AAAGAATGCA AATATTGAGA TTGCGTTAAC TGAACGAACT 
CTACTGGGCT TTTTCCTAGC AAAAATTCAT AAAATTGATC CAGATATTAT TGTAGGCCAT 
GACATTTATG GTTTTGATCT CGAAGTGTTG CTTCAAAGAA TTAGCTCGTG CAAGGTTCCT 
TTCTGGTCCA AAATCGGTAG ACTGAGAAGA TCTGTTATGC CAAAACTTGG GGGTCGAAGT 
GGCTTTGCAG AAAGGAATGC AGCTTGTGGT CGCATCATTT GCGATACTGA GATTTCTGCC 
AAGGAGTTAA TTCGATGTAA AAGCTATCAT TTGTCAGAAC TGGTACATCA AATATTGAAA 
ACAGAGAGAG TTTTGATGCC ACCAGAAAAC ATCCGCAATG CATACAGTGA TTCAGCACAT 
CTTCTTTACA TGCTGGAGAA CACCTGGACT GATGCAAAAT TCATCCTTCA GATCATGTGT 
GAGCTGAATG TTCTTCCCCT GGCCTTGCAG ATCACAAATA TTGCAGGCAA TGTCTTGTCA 
AGGACATTAA TGGGTGGACG CTCAGAAAGA AACGAGTACC TTCTCCTTCA TGCCTTCACA 
GAAAATAACT TCATAGTGCC AGACAAGCCA GTGTTCAAGA AAATTCAACA GACCACTGTG 
GAGGACGATG ATAATATGGA CACAGATCAA AATAAAAATA AGAAAGGTCG AAAGAAGGCT 
GCCTATGCTG GAGGATTGGT CCTGGAACCC AAAGTTGGTT TCTACGACAA ATTCATCTTG 
CTTCTGGATT TCAACAGCTT GTACCCCTCT ATCATTCAGG AATATAATAT CTGCTTTACA 
ACAGTGCAAA GAGAGGCTCC TACTTCACAG AAAGGTGAAG ATCAGGAAGA AATTCCAGAG 
CTACCTCATT CTGATCTTGA AATGGGGATC TTACCACGAG AGATACGGAA ACTGGTGGAG 
AGGAGGCGTC ACGTGAAGCA GTTAATGAAG CAGCCTGACC TAAACCCTGA TTTATACTTA 
CAGTATGACA TCAGACAAAA AGCTTTAAAG CTCACGGCAA ACAGCATGTA TGGATGCCTG 
GGCTTCTCCT ACAGCAGGTT CTATGCCAAA CCTTTGGCTG CATTGGTTAC TCACCAAGGG 
AGAGAGATTT TGCTGCATAC CAAGGAAATG GTACAGAAGA TGAATCTGGA AGTCATATAT 
GGAGACACGG ATTCTATCAT GATAAACACC AATTGTAATA ATTTAGAAGA AGTCTTCAAG 
CTTGGAAACC GGGTGAAAAG TGAGGTAAAC AAATCATACA AACTTTTAGA GATTGACATT 
GATGGTATCT TTAAATCACT CTTGCTACTT AAGAAAAAGA AGTATGCCGC TCTGACTGTG 
GAGCCTACAG GTGATGGAAA GTATGTTACT AAGCAGGAGC TTAAAGGTTT GGATATTGTC 
AGAAGAGACT GGTGTGAACT TGCTAAAGAA GCTGGAAATT ATGTTATCAG TCAGATTCTG 
TCTGATCAGC CACGTGACAA TATTGTTGAG AACATTCAGA AGAAATTAAC TGAAATTGGG 
GAGAATGTAA CAAATGGAAC TGTGCCCATT AGCCAGTATG AAATAAACAA GGCATTGACA 
AAGGATCCTC AGGATTACCC TGATAAAAAA AGCTTACCTC ACGTACATGT TGCCATGTGG 
ATAAATTCCC AAGGTGGAAG AAAGGTGAAA GCTGGTGATA CAATATCCTA TGTCATTTGC 
CAGGACGGAT CAAATCTTAG TGCCAGCCAG AGAGCATATG CTCCAGAGCA GCTGCAAAAA 
CAAGAGAACC TTTCCATTGA CACACAATAC TACCTGTCAC AGCAAGTCCA CCCAGTAGTG 
GCTCGGATCT GTGAGCCCAT TGATGGAATT GATTCTGCAC TCATTGCAAT GTGGTTAGGA 
CTTGACCCCT CCCAGTTCCG GGCTCACCGC CACCATCAGC AGGATGAGGA AAATGATGCA 
TTGTTGGGAG GTCCTTCCCA ACTCACAGAT GAGGAAAAAT ACAGAGATTG TGAACGATTC 
AAATTCTTCT GCCCAAAGTG TGGAACAGAG AACATTTATG ACAATGTTTT TGATGGCTCT 
GGGCTGCAGA TTGAACCCGG CTTGAAACGC TGCAGTAAAC CAGAGTGTGA TGCTTCTCCC 
TTGGACTACG TTATTCAGGT TCAAAACAAG CTGCTCTTGG ACATAAGACG TTACATAAAG 
AAATACTACA GTGGTTGGCT TGTATGTGAA GAAAAAACCT GCCAGAACAG AACACGACGA 
GTTCCTCTGA GCTTCTCCCG CAATGGGCCG ATATGCCAAG CTTGCACTAA AGCAACGCTA 
AGATCAGAGT ATCCGGAAAA GGCCCTGTAC ACTCAGCTTT GTTTCTATAG ATTCATTTTT 
GACTGGGATT ATGCACTGGA GAAAGTTGTT TCTGAACAAG AGAGAGGTCA CTTAAAAAAG 
AAGCATTTTC AAGAGTCAGA AAATCTGTAC AAGAAGTTAA AGAGCACTGT CGACCAAGTA 
CTTTCACAGA GCGGGTATTC AGAAGTGAAC CTCAGCAAGC TGTTCCAAAC ACTCAGCACC 
ATTAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004911811.1
CDS	166..4554
Translation	MSESGSFAASRSRREKTEKSGRKEALERLKRAKAGEKVKYEVEEISSIYEEVDEEQYSKIVRDRQDDDWIVDDDGTGYVEDGREIFDDDLEDNALADSGKRAKGAPKDKSNVKKSSVSKPNNIKSMFMASAVKRTTDKTVDLSKDDLLGDLLQDLKSQALPITPPPVITLKKKKLAGSPLNPFSVPPAAPKVLPVKKPPALAKLGHLSSAAPSNNSASSFVPREIKNEPNSDLISSAPLNVKTQVKEEDTSMVEFDDGDFDEPMEEDNLESPSVVSSTIKKEEPKIKCVKEDSVKMEESSFMTSAALNESCWDQIDEVESMTTEVQVDSSRLPLVTGADGSQVFKFYWLDAYEDQYSQPGVVYLFGKVWIETAEAYVSCCVSVKNIERTVYLLPRENRVQLSTGKDTGVPVSMMNVYQEFNESVAEKYKIMKFKSKKVEKDYAFEIPDVPASSEYLEVRYSADSPQLPQDLKGETFSHVFGTNTSSLELFLLSRKIKGPSWLEIKSPQLSSQPMSWCKVEAVVMRPDQVNVVKDLAPPPVVVLSLSMKTVQNAKTHQNEIVAMAALVHHKFPFDKAPPQPPFQTHFCVLSKPNDCIFPYDFKEAVKRKNANIEIALTERTLLGFFLAKIHKIDPDIIVGHDIYGFDLEVLLQRISSCKVPFWSKIGRLRRSVMPKLGGRSGFAERNAACGRIICDTEISAKELIRCKSYHLSELVHQILKTERVLMPPENIRNAYSDSAHLLYMLENTWTDAKFILQIMCELNVLPLALQITNIAGNVLSRTLMGGRSERNEYLLLHAFTENNFIVPDKPVFKKIQQTTVEDDDNMDTDQNKNKKGRKKAAYAGGLVLEPKVGFYDKFILLLDFNSLYPSIIQEYNICFTTVQREAPTSQKGEDQEEIPELPHSDLEMGILPREIRKLVERRRHVKQLMKQPDLNPDLYLQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTHQGREILLHTKEMVQKMNLEVIYGDTDSIMINTNCNNLEEVFKLGNRVKSEVNKSYKLLEIDIDGIFKSLLLLKKKKYAALTVEPTGDGKYVTKQELKGLDIVRRDWCELAKEAGNYVISQILSDQPRDNIVENIQKKLTEIGENVTNGTVPISQYEINKALTKDPQDYPDKKSLPHVHVAMWINSQGGRKVKAGDTISYVICQDGSNLSASQRAYAPEQLQKQENLSIDTQYYLSQQVHPVVARICEPIDGIDSALIAMWLGLDPSQFRAHRHHQQDEENDALLGGPSQLTDEEKYRDCERFKFFCPKCGTENIYDNVFDGSGLQIEPGLKRCSKPECDASPLDYVIQVQNKLLLDIRRYIKKYYSGWLVCEEKTCQNRTRRVPLSFSRNGPICQACTKATLRSEYPEKALYTQLCFYRFIFDWDYALEKVVSEQERGHLKKKHFQESENLYKKLKSTVDQVLSQSGYSEVNLSKLFQTLSTIK

Target ORF information:

RefSeq Version	XM_004911754.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis polymerase (DNA directed), alpha 1, catalytic subunit (pola1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911754.3

ORF Insert Sequence:

ATGTCAGAAT CTGGAAGTTT TGCAGCATCA CGTTCGCGGC GTGAAAAAAC AGAAAAGAGC 
GGCCGTAAAG AAGCATTGGA ACGTTTGAAA AGAGCAAAAG CTGGTGAAAA AGTCAAATAT 
GAGGTGGAAG AAATTTCAAG CATTTATGAG GAGGTGGATG AAGAACAATA CTCTAAAATT 
GTTCGTGACC GACAGGATGA TGATTGGATA GTGGATGATG ATGGGACCGG TTATGTAGAA 
GATGGAAGAG AGATTTTTGA TGATGACTTA GAAGACAATG CTCTTGCTGA TTCAGGTAAA 
AGAGCTAAGG GAGCACCCAA AGATAAATCT AATGTAAAAA AGTCATCGGT GTCGAAGCCA 
AATAATATCA AGTCAATGTT TATGGCCAGT GCTGTCAAAA GGACAACAGA TAAAACTGTG 
GATCTGTCAA AAGATGACTT GCTTGGTGAT TTACTGCAAG ATTTAAAGTC ACAGGCTCTT 
CCTATAACAC CACCCCCAGT AATTACTCTA AAGAAGAAGA AACTTGCAGG GTCTCCTTTA 
AACCCTTTCT CGGTACCACC TGCTGCCCCA AAGGTTTTAC CTGTGAAAAA ACCACCAGCT 
TTAGCAAAGC TTGGGCATCT CTCAAGTGCT GCGCCATCTA ACAACTCTGC CAGTTCTTTT 
GTACCCCGGG AAATTAAGAA TGAGCCAAAT TCTGATTTGA TTTCTTCTGC TCCTTTGAAT 
GTGAAAACTC AAGTAAAAGA AGAGGATACG TCAATGGTTG AGTTCGATGA TGGAGATTTT 
GATGAACCAA TGGAAGAAGA CAATTTGGAA TCTCCTTCAG TTGTTTCTAG CACAATTAAG 
AAGGAAGAAC CAAAAATTAA GTGTGTGAAA GAGGATAGTG TTAAAATGGA GGAAAGTTCT 
TTCATGACTT CCGCTGCTTT AAATGAATCT TGTTGGGATC AAATTGATGA GGTTGAATCA 
ATGACCACTG AAGTTCAGGT AGATTCAAGC CGCCTACCCT TGGTTACAGG TGCAGATGGA 
AGTCAGGTGT TTAAGTTTTA TTGGCTAGAT GCCTATGAAG ACCAGTACAG TCAGCCAGGA 
GTAGTCTACT TATTTGGAAA AGTTTGGATA GAGACAGCTG AGGCGTATGT CAGCTGCTGT 
GTTAGTGTGA AGAACATTGA GAGGACTGTA TATCTCCTAC CTCGTGAAAA TAGAGTGCAG 
CTTTCTACAG GCAAAGACAC TGGAGTACCA GTGTCTATGA TGAATGTATA CCAGGAGTTT 
AATGAAAGTG TTGCAGAAAA GTACAAAATC ATGAAGTTCA AGTCTAAGAA AGTTGAGAAG 
GATTATGCTT TTGAAATTCC TGATGTTCCA GCCAGCTCTG AATATCTAGA GGTTCGATAT 
TCTGCGGACT CACCTCAACT TCCTCAGGAT CTAAAAGGAG AAACATTTTC ACATGTGTTT 
GGAACAAATA CTTCAAGCCT GGAGCTTTTC TTGCTTAGCA GAAAAATAAA AGGGCCCAGC 
TGGCTGGAGA TAAAAAGCCC ACAGCTGAGC AGTCAGCCCA TGAGCTGGTG CAAGGTTGAG 
GCAGTGGTGA TGAGGCCTGA CCAAGTGAAT GTTGTGAAGG ATCTGGCACC TCCACCTGTT 
GTTGTTTTGT CGCTCAGTAT GAAGACTGTG CAGAATGCAA AGACTCACCA AAATGAGATT 
GTGGCAATGG CTGCTTTGGT TCATCACAAA TTTCCCTTTG ATAAAGCACC ACCACAGCCA 
CCATTCCAGA CACATTTTTG TGTTCTGAGC AAACCTAATG ATTGCATTTT CCCATACGAC 
TTTAAGGAAG CGGTGAAACG AAAGAATGCA AATATTGAGA TTGCGTTAAC TGAACGAACT 
CTACTGGGCT TTTTCCTAGC AAAAATTCAT AAAATTGATC CAGATATTAT TGTAGGCCAT 
GACATTTATG GTTTTGATCT CGAAGTGTTG CTTCAAAGAA TTAGCTCGTG CAAGGTTCCT 
TTCTGGTCCA AAATCGGTAG ACTGAGAAGA TCTGTTATGC CAAAACTTGG GGGTCGAAGT 
GGCTTTGCAG AAAGGAATGC AGCTTGTGGT CGCATCATTT GCGATACTGA GATTTCTGCC 
AAGGAGTTAA TTCGATGTAA AAGCTATCAT TTGTCAGAAC TGGTACATCA AATATTGAAA 
ACAGAGAGAG TTTTGATGCC ACCAGAAAAC ATCCGCAATG CATACAGTGA TTCAGCACAT 
CTTCTTTACA TGCTGGAGAA CACCTGGACT GATGCAAAAT TCATCCTTCA GATCATGTGT 
GAGCTGAATG TTCTTCCCCT GGCCTTGCAG ATCACAAATA TTGCAGGCAA TGTCTTGTCA 
AGGACATTAA TGGGTGGACG CTCAGAAAGA AACGAGTACC TTCTCCTTCA TGCCTTCACA 
GAAAATAACT TCATAGTGCC AGACAAGCCA GTGTTCAAGA AAATTCAACA GACCACTGTG 
GAGGACGATG ATAATATGGA CACAGATCAA AATAAAAATA AGAAAGGTCG AAAGAAGGCT 
GCCTATGCTG GAGGATTGGT CCTGGAACCC AAAGTTGGTT TCTACGACAA ATTCATCTTG 
CTTCTGGATT TCAACAGCTT GTACCCCTCT ATCATTCAGG AATATAATAT CTGCTTTACA 
ACAGTGCAAA GAGAGGCTCC TACTTCACAG AAAGGTGAAG ATCAGGAAGA AATTCCAGAG 
CTACCTCATT CTGATCTTGA AATGGGGATC TTACCACGAG AGATACGGAA ACTGGTGGAG 
AGGAGGCGTC ACGTGAAGCA GTTAATGAAG CAGCCTGACC TAAACCCTGA TTTATACTTA 
CAGTATGACA TCAGACAAAA AGCTTTAAAG CTCACGGCAA ACAGCATGTA TGGATGCCTG 
GGCTTCTCCT ACAGCAGGTT CTATGCCAAA CCTTTGGCTG CATTGGTTAC TCACCAAGGG 
AGAGAGATTT TGCTGCATAC CAAGGAAATG GTACAGAAGA TGAATCTGGA AGTCATATAT 
GGAGACACGG ATTCTATCAT GATAAACACC AATTGTAATA ATTTAGAAGA AGTCTTCAAG 
CTTGGAAACC GGGTGAAAAG TGAGGTAAAC AAATCATACA AACTTTTAGA GATTGACATT 
GATGGTATCT TTAAATCACT CTTGCTACTT AAGAAAAAGA AGTATGCCGC TCTGACTGTG 
GAGCCTACAG GTGATGGAAA GTATGTTACT AAGCAGGAGC TTAAAGGTTT GGATATTGTC 
AGAAGAGACT GGTGTGAACT TGCTAAAGAA GCTGGAAATT ATGTTATCAG TCAGATTCTG 
TCTGATCAGC CACGTGACAA TATTGTTGAG AACATTCAGA AGAAATTAAC TGAAATTGGG 
GAGAATGTAA CAAATGGAAC TGTGCCCATT AGCCAGTATG AAATAAACAA GGCATTGACA 
AAGGATCCTC AGGATTACCC TGATAAAAAA AGCTTACCTC ACGTACATGT TGCCATGTGG 
ATAAATTCCC AAGGTGGAAG AAAGGTGAAA GCTGGTGATA CAATATCCTA TGTCATTTGC 
CAGGACGGAT CAAATCTTAG TGCCAGCCAG AGAGCATATG CTCCAGAGCA GCTGCAAAAA 
CAAGAGAACC TTTCCATTGA CACACAATAC TACCTGTCAC AGCAAGTCCA CCCAGTAGTG 
GCTCGGATCT GTGAGCCCAT TGATGGAATT GATTCTGCAC TCATTGCAAT GTGGTTAGGA 
CTTGACCCCT CCCAGTTCCG GGCTCACCGC CACCATCAGC AGGATGAGGA AAATGATGCA 
TTGTTGGGAG GTCCTTCCCA ACTCACAGAT GAGGAAAAAT ACAGAGATTG TGAACGATTC 
AAATTCTTCT GCCCAAAGTG TGGAACAGAG AACATTTATG ACAATGTTTT TGATGGCTCT 
GGGCTGCAGA TTGAACCCGG CTTGAAACGC TGCAGTAAAC CAGAGTGTGA TGCTTCTCCC 
TTGGACTACG TTATTCAGGT TCAAAACAAG CTGCTCTTGG ACATAAGACG TTACATAAAG 
AAATACTACA GTGGTTGGCT TGTATGTGAA GAAAAAACCT GCCAGAACAG AACACGACGA 
GTTCCTCTGA GCTTCTCCCG CAATGGGCCG ATATGCCAAG CTTGCACTAA AGCAACGCTA 
AGATCAGAGT ATCCGGAAAA GGCCCTGTAC ACTCAGCTTT GTTTCTATAG ATTCATTTTT 
GACTGGGATT ATGCACTGGA GAAAGTTGTT TCTGAACAAG AGAGAGGTCA CTTAAAAAAG 
AAGCATTTTC AAGAGTCAGA AAATCTGTAC AAGAAGTTAA AGAGCACTGT CGACCAAGTA 
CTTTCACAGA GCGGGTATTC AGAAGTGAAC CTCAGCAAGC TGTTCCAAAC ACTCAGCACC 
ATTAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pola1 ( XM_012957632.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012813086.1 Xenopus tropicalis polymerase (DNA directed), alpha 1, catalytic subunit (pola1), transcript variant X1, mRNA.

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CloneID	OXa33001
Clone ID Related Accession (Same CDS sequence)	XM_012957632.2
Accession Version	XM_012957632.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4443bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA polymerase alpha catalytic subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957632.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTTGG TGTTCTGTGC GCCCCGCAGT ACCGTCTTCC CTCCTTACCT TGACATGTCA 
GAATCTGGAA GTTTTGCAGC ATCACGTTCG CGGCGTGAAA AAACAGAAAA GAGCGGCCGT 
AAAGAAGCAT TGGAACGTTT GAAAAGAGCA AAAGCTGGTG AAAAAGTCAA ATATGAGGTG 
GAAGAAATTT CAAGCATTTA TGAGGAGGTG GATGAAGAAC AATACTCTAA AATTGTTCGT 
GACCGACAGG ATGATGATTG GATAGTGGAT GATGATGGGA CCGGTTATGT AGAAGATGGA 
AGAGAGATTT TTGATGATGA CTTAGAAGAC AATGCTCTTG CTGATTCAGG TAAAAGAGCT 
AAGGGAGCAC CCAAAGATAA ATCTAATGTA AAAAAGTCAT CGGTGTCGAA GCCAAATAAT 
ATCAAGTCAA TGTTTATGGC CAGTGCTGTC AAAAGGACAA CAGATAAAAC TGTGGATCTG 
TCAAAAGATG ACTTGCTTGG TGATTTACTG CAAGATTTAA AGTCACAGGC TCTTCCTATA 
ACACCACCCC CAGTAATTAC TCTAAAGAAG AAGAAACTTG CAGGGTCTCC TTTAAACCCT 
TTCTCGGTAC CACCTGCTGC CCCAAAGGTT TTACCTGTGA AAAAACCACC AGCTTTAGCA 
AAGCTTGGGC ATCTCTCAAG TGCTGCGCCA TCTAACAACT CTGCCAGTTC TTTTGTACCC 
CGGGAAATTA AGAATGAGCC AAATTCTGAT TTGATTTCTT CTGCTCCTTT GAATGTGAAA 
ACTCAAGTAA AAGAAGAGGA TACGTCAATG GTTGAGTTCG ATGATGGAGA TTTTGATGAA 
CCAATGGAAG AAGACAATTT GGAATCTCCT TCAGTTGTTT CTAGCACAAT TAAGAAGGAA 
GAACCAAAAA TTAAGTGTGT GAAAGAGGAT AGTGTTAAAA TGGAGGAAAG TTCTTTCATG 
ACTTCCGCTG CTTTAAATGA ATCTTGTTGG GATCAAATTG ATGAGGTTGA ATCAATGACC 
ACTGAAGTTC AGGTAGATTC AAGCCGCCTA CCCTTGGTTA CAGGTGCAGA TGGAAGTCAG 
GTGTTTAAGT TTTATTGGCT AGATGCCTAT GAAGACCAGT ACAGTCAGCC AGGAGTAGTC 
TACTTATTTG GAAAAGTTTG GATAGAGACA GCTGAGGCGT ATGTCAGCTG CTGTGTTAGT 
GTGAAGAACA TTGAGAGGAC TGTATATCTC CTACCTCGTG AAAATAGAGT GCAGCTTTCT 
ACAGGCAAAG ACACTGGAGT ACCAGTGTCT ATGATGAATG TATACCAGGA GTTTAATGAA 
AGTGTTGCAG AAAAGTACAA AATCATGAAG TTCAAGTCTA AGAAAGTTGA GAAGGATTAT 
GCTTTTGAAA TTCCTGATGT TCCAGCCAGC TCTGAATATC TAGAGGTTCG ATATTCTGCG 
GACTCACCTC AACTTCCTCA GGATCTAAAA GGAGAAACAT TTTCACATGT GTTTGGAACA 
AATACTTCAA GCCTGGAGCT TTTCTTGCTT AGCAGAAAAA TAAAAGGGCC CAGCTGGCTG 
GAGATAAAAA GCCCACAGCT GAGCAGTCAG CCCATGAGCT GGTGCAAGGT TGAGGCAGTG 
GTGATGAGGC CTGACCAAGT GAATGTTGTG AAGGATCTGG CACCTCCACC TGTTGTTGTT 
TTGTCGCTCA GTATGAAGAC TGTGCAGAAT GCAAAGACTC ACCAAAATGA GATTGTGGCA 
ATGGCTGCTT TGGTTCATCA CAAATTTCCC TTTGATAAAG CACCACCACA GCCACCATTC 
CAGACACATT TTTGTGTTCT GAGCAAACCT AATGATTGCA TTTTCCCATA CGACTTTAAG 
GAAGCGGTGA AACGAAAGAA TGCAAATATT GAGATTGCGT TAACTGAACG AACTCTACTG 
GGCTTTTTCC TAGCAAAAAT TCATAAAATT GATCCAGATA TTATTGTAGG CCATGACATT 
TATGGTTTTG ATCTCGAAGT GTTGCTTCAA AGAATTAGCT CGTGCAAGGT TCCTTTCTGG 
TCCAAAATCG GTAGACTGAG AAGATCTGTT ATGCCAAAAC TTGGGGGTCG AAGTGGCTTT 
GCAGAAAGGA ATGCAGCTTG TGGTCGCATC ATTTGCGATA CTGAGATTTC TGCCAAGGAG 
TTAATTCGAT GTAAAAGCTA TCATTTGTCA GAACTGGTAC ATCAAATATT GAAAACAGAG 
AGAGTTTTGA TGCCACCAGA AAACATCCGC AATGCATACA GTGATTCAGC ACATCTTCTT 
TACATGCTGG AGAACACCTG GACTGATGCA AAATTCATCC TTCAGATCAT GTGTGAGCTG 
AATGTTCTTC CCCTGGCCTT GCAGATCACA AATATTGCAG GCAATGTCTT GTCAAGGACA 
TTAATGGGTG GACGCTCAGA AAGAAACGAG TACCTTCTCC TTCATGCCTT CACAGAAAAT 
AACTTCATAG TGCCAGACAA GCCAGTGTTC AAGAAAATTC AACAGACCAC TGTGGAGGAC 
GATGATAATA TGGACACAGA TCAAAATAAA AATAAGAAAG GTCGAAAGAA GGCTGCCTAT 
GCTGGAGGAT TGGTCCTGGA ACCCAAAGTT GGTTTCTACG ACAAATTCAT CTTGCTTCTG 
GATTTCAACA GCTTGTACCC CTCTATCATT CAGGAATATA ATATCTGCTT TACAACAGTG 
CAAAGAGAGG CTCCTACTTC ACAGAAAGGT GAAGATCAGG AAGAAATTCC AGAGCTACCT 
CATTCTGATC TTGAAATGGG GATCTTACCA CGAGAGATAC GGAAACTGGT GGAGAGGAGG 
CGTCACGTGA AGCAGTTAAT GAAGCAGCCT GACCTAAACC CTGATTTATA CTTACAGTAT 
GACATCAGAC AAAAAGCTTT AAAGCTCACG GCAAACAGCA TGTATGGATG CCTGGGCTTC 
TCCTACAGCA GGTTCTATGC CAAACCTTTG GCTGCATTGG TTACTCACCA AGGGAGAGAG 
ATTTTGCTGC ATACCAAGGA AATGGTACAG AAGATGAATC TGGAAGTCAT ATATGGAGAC 
ACGGATTCTA TCATGATAAA CACCAATTGT AATAATTTAG AAGAAGTCTT CAAGCTTGGA 
AACCGGGTGA AAAGTGAGGT AAACAAATCA TACAAACTTT TAGAGATTGA CATTGATGGT 
ATCTTTAAAT CACTCTTGCT ACTTAAGAAA AAGAAGTATG CCGCTCTGAC TGTGGAGCCT 
ACAGGTGATG GAAAGTATGT TACTAAGCAG GAGCTTAAAG GTTTGGATAT TGTCAGAAGA 
GACTGGTGTG AACTTGCTAA AGAAGCTGGA AATTATGTTA TCAGTCAGAT TCTGTCTGAT 
CAGCCACGTG ACAATATTGT TGAGAACATT CAGAAGAAAT TAACTGAAAT TGGGGAGAAT 
GTAACAAATG GAACTGTGCC CATTAGCCAG TATGAAATAA ACAAGGCATT GACAAAGGAT 
CCTCAGGATT ACCCTGATAA AAAAAGCTTA CCTCACGTAC ATGTTGCCAT GTGGATAAAT 
TCCCAAGGTG GAAGAAAGGT GAAAGCTGGT GATACAATAT CCTATGTCAT TTGCCAGGAC 
GGATCAAATC TTAGTGCCAG CCAGAGAGCA TATGCTCCAG AGCAGCTGCA AAAACAAGAG 
AACCTTTCCA TTGACACACA ATACTACCTG TCACAGCAAG TCCACCCAGT AGTGGCTCGG 
ATCTGTGAGC CCATTGATGG AATTGATTCT GCACTCATTG CAATGTGGTT AGGACTTGAC 
CCCTCCCAGT TCCGGGCTCA CCGCCACCAT CAGCAGGATG AGGAAAATGA TGCATTGTTG 
GGAGGTCCTT CCCAACTCAC AGATGAGGAA AAATACAGAG ATTGTGAACG ATTCAAATTC 
TTCTGCCCAA AGTGTGGAAC AGAGAACATT TATGACAATG TTTTTGATGG CTCTGGGCTG 
CAGATTGAAC CCGGCTTGAA ACGCTGCAGT AAACCAGAGT GTGATGCTTC TCCCTTGGAC 
TACGTTATTC AGGTTCAAAA CAAGCTGCTC TTGGACATAA GACGTTACAT AAAGAAATAC 
TACAGTGGTT GGCTTGTATG TGAAGAAAAA ACCTGCCAGA ACAGAACACG ACGAGTTCCT 
CTGAGCTTCT CCCGCAATGG GCCGATATGC CAAGCTTGCA CTAAAGCAAC GCTAAGATCA 
GAGTATCCGG AAAAGGCCCT GTACACTCAG CTTTGTTTCT ATAGATTCAT TTTTGACTGG 
GATTATGCAC TGGAGAAAGT TGTTTCTGAA CAAGAGAGAG GTCACTTAAA AAAGAAGCAT 
TTTCAAGAGT CAGAAAATCT GTACAAGAAG TTAAAGAGCA CTGTCGACCA AGTACTTTCA 
CAGAGCGGGT ATTCAGAAGT GAACCTCAGC AAGCTGTTCC AAACACTCAG CACCATTAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012813086.1
CDS	48..4490
Translation	MHLVFCAPRSTVFPPYLDMSESGSFAASRSRREKTEKSGRKEALERLKRAKAGEKVKYEVEEISSIYEEVDEEQYSKIVRDRQDDDWIVDDDGTGYVEDGREIFDDDLEDNALADSGKRAKGAPKDKSNVKKSSVSKPNNIKSMFMASAVKRTTDKTVDLSKDDLLGDLLQDLKSQALPITPPPVITLKKKKLAGSPLNPFSVPPAAPKVLPVKKPPALAKLGHLSSAAPSNNSASSFVPREIKNEPNSDLISSAPLNVKTQVKEEDTSMVEFDDGDFDEPMEEDNLESPSVVSSTIKKEEPKIKCVKEDSVKMEESSFMTSAALNESCWDQIDEVESMTTEVQVDSSRLPLVTGADGSQVFKFYWLDAYEDQYSQPGVVYLFGKVWIETAEAYVSCCVSVKNIERTVYLLPRENRVQLSTGKDTGVPVSMMNVYQEFNESVAEKYKIMKFKSKKVEKDYAFEIPDVPASSEYLEVRYSADSPQLPQDLKGETFSHVFGTNTSSLELFLLSRKIKGPSWLEIKSPQLSSQPMSWCKVEAVVMRPDQVNVVKDLAPPPVVVLSLSMKTVQNAKTHQNEIVAMAALVHHKFPFDKAPPQPPFQTHFCVLSKPNDCIFPYDFKEAVKRKNANIEIALTERTLLGFFLAKIHKIDPDIIVGHDIYGFDLEVLLQRISSCKVPFWSKIGRLRRSVMPKLGGRSGFAERNAACGRIICDTEISAKELIRCKSYHLSELVHQILKTERVLMPPENIRNAYSDSAHLLYMLENTWTDAKFILQIMCELNVLPLALQITNIAGNVLSRTLMGGRSERNEYLLLHAFTENNFIVPDKPVFKKIQQTTVEDDDNMDTDQNKNKKGRKKAAYAGGLVLEPKVGFYDKFILLLDFNSLYPSIIQEYNICFTTVQREAPTSQKGEDQEEIPELPHSDLEMGILPREIRKLVERRRHVKQLMKQPDLNPDLYLQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTHQGREILLHTKEMVQKMNLEVIYGDTDSIMINTNCNNLEEVFKLGNRVKSEVNKSYKLLEIDIDGIFKSLLLLKKKKYAALTVEPTGDGKYVTKQELKGLDIVRRDWCELAKEAGNYVISQILSDQPRDNIVENIQKKLTEIGENVTNGTVPISQYEINKALTKDPQDYPDKKSLPHVHVAMWINSQGGRKVKAGDTISYVICQDGSNLSASQRAYAPEQLQKQENLSIDTQYYLSQQVHPVVARICEPIDGIDSALIAMWLGLDPSQFRAHRHHQQDEENDALLGGPSQLTDEEKYRDCERFKFFCPKCGTENIYDNVFDGSGLQIEPGLKRCSKPECDASPLDYVIQVQNKLLLDIRRYIKKYYSGWLVCEEKTCQNRTRRVPLSFSRNGPICQACTKATLRSEYPEKALYTQLCFYRFIFDWDYALEKVVSEQERGHLKKKHFQESENLYKKLKSTVDQVLSQSGYSEVNLSKLFQTLSTIK

Target ORF information:

RefSeq Version	XM_012957632.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis polymerase (DNA directed), alpha 1, catalytic subunit (pola1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957632.2

ORF Insert Sequence:

ATGCACTTGG TGTTCTGTGC GCCCCGCAGT ACCGTCTTCC CTCCTTACCT TGACATGTCA 
GAATCTGGAA GTTTTGCAGC ATCACGTTCG CGGCGTGAAA AAACAGAAAA GAGCGGCCGT 
AAAGAAGCAT TGGAACGTTT GAAAAGAGCA AAAGCTGGTG AAAAAGTCAA ATATGAGGTG 
GAAGAAATTT CAAGCATTTA TGAGGAGGTG GATGAAGAAC AATACTCTAA AATTGTTCGT 
GACCGACAGG ATGATGATTG GATAGTGGAT GATGATGGGA CCGGTTATGT AGAAGATGGA 
AGAGAGATTT TTGATGATGA CTTAGAAGAC AATGCTCTTG CTGATTCAGG TAAAAGAGCT 
AAGGGAGCAC CCAAAGATAA ATCTAATGTA AAAAAGTCAT CGGTGTCGAA GCCAAATAAT 
ATCAAGTCAA TGTTTATGGC CAGTGCTGTC AAAAGGACAA CAGATAAAAC TGTGGATCTG 
TCAAAAGATG ACTTGCTTGG TGATTTACTG CAAGATTTAA AGTCACAGGC TCTTCCTATA 
ACACCACCCC CAGTAATTAC TCTAAAGAAG AAGAAACTTG CAGGGTCTCC TTTAAACCCT 
TTCTCGGTAC CACCTGCTGC CCCAAAGGTT TTACCTGTGA AAAAACCACC AGCTTTAGCA 
AAGCTTGGGC ATCTCTCAAG TGCTGCGCCA TCTAACAACT CTGCCAGTTC TTTTGTACCC 
CGGGAAATTA AGAATGAGCC AAATTCTGAT TTGATTTCTT CTGCTCCTTT GAATGTGAAA 
ACTCAAGTAA AAGAAGAGGA TACGTCAATG GTTGAGTTCG ATGATGGAGA TTTTGATGAA 
CCAATGGAAG AAGACAATTT GGAATCTCCT TCAGTTGTTT CTAGCACAAT TAAGAAGGAA 
GAACCAAAAA TTAAGTGTGT GAAAGAGGAT AGTGTTAAAA TGGAGGAAAG TTCTTTCATG 
ACTTCCGCTG CTTTAAATGA ATCTTGTTGG GATCAAATTG ATGAGGTTGA ATCAATGACC 
ACTGAAGTTC AGGTAGATTC AAGCCGCCTA CCCTTGGTTA CAGGTGCAGA TGGAAGTCAG 
GTGTTTAAGT TTTATTGGCT AGATGCCTAT GAAGACCAGT ACAGTCAGCC AGGAGTAGTC 
TACTTATTTG GAAAAGTTTG GATAGAGACA GCTGAGGCGT ATGTCAGCTG CTGTGTTAGT 
GTGAAGAACA TTGAGAGGAC TGTATATCTC CTACCTCGTG AAAATAGAGT GCAGCTTTCT 
ACAGGCAAAG ACACTGGAGT ACCAGTGTCT ATGATGAATG TATACCAGGA GTTTAATGAA 
AGTGTTGCAG AAAAGTACAA AATCATGAAG TTCAAGTCTA AGAAAGTTGA GAAGGATTAT 
GCTTTTGAAA TTCCTGATGT TCCAGCCAGC TCTGAATATC TAGAGGTTCG ATATTCTGCG 
GACTCACCTC AACTTCCTCA GGATCTAAAA GGAGAAACAT TTTCACATGT GTTTGGAACA 
AATACTTCAA GCCTGGAGCT TTTCTTGCTT AGCAGAAAAA TAAAAGGGCC CAGCTGGCTG 
GAGATAAAAA GCCCACAGCT GAGCAGTCAG CCCATGAGCT GGTGCAAGGT TGAGGCAGTG 
GTGATGAGGC CTGACCAAGT GAATGTTGTG AAGGATCTGG CACCTCCACC TGTTGTTGTT 
TTGTCGCTCA GTATGAAGAC TGTGCAGAAT GCAAAGACTC ACCAAAATGA GATTGTGGCA 
ATGGCTGCTT TGGTTCATCA CAAATTTCCC TTTGATAAAG CACCACCACA GCCACCATTC 
CAGACACATT TTTGTGTTCT GAGCAAACCT AATGATTGCA TTTTCCCATA CGACTTTAAG 
GAAGCGGTGA AACGAAAGAA TGCAAATATT GAGATTGCGT TAACTGAACG AACTCTACTG 
GGCTTTTTCC TAGCAAAAAT TCATAAAATT GATCCAGATA TTATTGTAGG CCATGACATT 
TATGGTTTTG ATCTCGAAGT GTTGCTTCAA AGAATTAGCT CGTGCAAGGT TCCTTTCTGG 
TCCAAAATCG GTAGACTGAG AAGATCTGTT ATGCCAAAAC TTGGGGGTCG AAGTGGCTTT 
GCAGAAAGGA ATGCAGCTTG TGGTCGCATC ATTTGCGATA CTGAGATTTC TGCCAAGGAG 
TTAATTCGAT GTAAAAGCTA TCATTTGTCA GAACTGGTAC ATCAAATATT GAAAACAGAG 
AGAGTTTTGA TGCCACCAGA AAACATCCGC AATGCATACA GTGATTCAGC ACATCTTCTT 
TACATGCTGG AGAACACCTG GACTGATGCA AAATTCATCC TTCAGATCAT GTGTGAGCTG 
AATGTTCTTC CCCTGGCCTT GCAGATCACA AATATTGCAG GCAATGTCTT GTCAAGGACA 
TTAATGGGTG GACGCTCAGA AAGAAACGAG TACCTTCTCC TTCATGCCTT CACAGAAAAT 
AACTTCATAG TGCCAGACAA GCCAGTGTTC AAGAAAATTC AACAGACCAC TGTGGAGGAC 
GATGATAATA TGGACACAGA TCAAAATAAA AATAAGAAAG GTCGAAAGAA GGCTGCCTAT 
GCTGGAGGAT TGGTCCTGGA ACCCAAAGTT GGTTTCTACG ACAAATTCAT CTTGCTTCTG 
GATTTCAACA GCTTGTACCC CTCTATCATT CAGGAATATA ATATCTGCTT TACAACAGTG 
CAAAGAGAGG CTCCTACTTC ACAGAAAGGT GAAGATCAGG AAGAAATTCC AGAGCTACCT 
CATTCTGATC TTGAAATGGG GATCTTACCA CGAGAGATAC GGAAACTGGT GGAGAGGAGG 
CGTCACGTGA AGCAGTTAAT GAAGCAGCCT GACCTAAACC CTGATTTATA CTTACAGTAT 
GACATCAGAC AAAAAGCTTT AAAGCTCACG GCAAACAGCA TGTATGGATG CCTGGGCTTC 
TCCTACAGCA GGTTCTATGC CAAACCTTTG GCTGCATTGG TTACTCACCA AGGGAGAGAG 
ATTTTGCTGC ATACCAAGGA AATGGTACAG AAGATGAATC TGGAAGTCAT ATATGGAGAC 
ACGGATTCTA TCATGATAAA CACCAATTGT AATAATTTAG AAGAAGTCTT CAAGCTTGGA 
AACCGGGTGA AAAGTGAGGT AAACAAATCA TACAAACTTT TAGAGATTGA CATTGATGGT 
ATCTTTAAAT CACTCTTGCT ACTTAAGAAA AAGAAGTATG CCGCTCTGAC TGTGGAGCCT 
ACAGGTGATG GAAAGTATGT TACTAAGCAG GAGCTTAAAG GTTTGGATAT TGTCAGAAGA 
GACTGGTGTG AACTTGCTAA AGAAGCTGGA AATTATGTTA TCAGTCAGAT TCTGTCTGAT 
CAGCCACGTG ACAATATTGT TGAGAACATT CAGAAGAAAT TAACTGAAAT TGGGGAGAAT 
GTAACAAATG GAACTGTGCC CATTAGCCAG TATGAAATAA ACAAGGCATT GACAAAGGAT 
CCTCAGGATT ACCCTGATAA AAAAAGCTTA CCTCACGTAC ATGTTGCCAT GTGGATAAAT 
TCCCAAGGTG GAAGAAAGGT GAAAGCTGGT GATACAATAT CCTATGTCAT TTGCCAGGAC 
GGATCAAATC TTAGTGCCAG CCAGAGAGCA TATGCTCCAG AGCAGCTGCA AAAACAAGAG 
AACCTTTCCA TTGACACACA ATACTACCTG TCACAGCAAG TCCACCCAGT AGTGGCTCGG 
ATCTGTGAGC CCATTGATGG AATTGATTCT GCACTCATTG CAATGTGGTT AGGACTTGAC 
CCCTCCCAGT TCCGGGCTCA CCGCCACCAT CAGCAGGATG AGGAAAATGA TGCATTGTTG 
GGAGGTCCTT CCCAACTCAC AGATGAGGAA AAATACAGAG ATTGTGAACG ATTCAAATTC 
TTCTGCCCAA AGTGTGGAAC AGAGAACATT TATGACAATG TTTTTGATGG CTCTGGGCTG 
CAGATTGAAC CCGGCTTGAA ACGCTGCAGT AAACCAGAGT GTGATGCTTC TCCCTTGGAC 
TACGTTATTC AGGTTCAAAA CAAGCTGCTC TTGGACATAA GACGTTACAT AAAGAAATAC 
TACAGTGGTT GGCTTGTATG TGAAGAAAAA ACCTGCCAGA ACAGAACACG ACGAGTTCCT 
CTGAGCTTCT CCCGCAATGG GCCGATATGC CAAGCTTGCA CTAAAGCAAC GCTAAGATCA 
GAGTATCCGG AAAAGGCCCT GTACACTCAG CTTTGTTTCT ATAGATTCAT TTTTGACTGG 
GATTATGCAC TGGAGAAAGT TGTTTCTGAA CAAGAGAGAG GTCACTTAAA AAAGAAGCAT 
TTTCAAGAGT CAGAAAATCT GTACAAGAAG TTAAAGAGCA CTGTCGACCA AGTACTTTCA 
CAGAGCGGGT ATTCAGAAGT GAACCTCAGC AAGCTGTTCC AAACACTCAG CACCATTAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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pola1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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