pola1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

  • Gene

  • Clones

  • gRNAs

The following pola1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pola1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa42678 XM_018091470.1
Latest version!
Xenopus tropicalis polymerase (DNA directed), alpha 1, catalytic subunit (pola1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OXa52095 XM_031896702.1
Latest version!
Xenopus tropicalis polymerase (DNA directed), alpha 1, catalytic subunit (pola1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa52096 XM_012957632.3
Latest version!
Xenopus tropicalis polymerase (DNA directed), alpha 1, catalytic subunit (pola1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa52097 XM_031896703.1
Latest version!
Xenopus tropicalis polymerase (DNA directed), alpha 1, catalytic subunit (pola1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa52097 XM_031896704.1
Latest version!
Xenopus tropicalis polymerase (DNA directed), alpha 1, catalytic subunit (pola1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa05773 XM_002933612.4
Latest version!
Xenopus tropicalis polymerase (DNA directed), alpha 1, catalytic subunit (pola1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa05773 XM_004911754.3
Latest version!
Xenopus tropicalis polymerase (DNA directed), alpha 1, catalytic subunit (pola1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa33001 XM_012957632.2
Latest version!
Xenopus tropicalis polymerase (DNA directed), alpha 1, catalytic subunit (pola1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa42678
    Clone ID Related Accession (Same CDS sequence) XM_018091470.1
    Accession Version XM_018091470.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4137bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA polymerase alpha catalytic subunit isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGCACTTGG TGTTCTGTGC GCCCCGCAGT ACCGTCTTCC CTCCTTACCT TGACATGTCA 
    GAATCTGGAA GTTTTGCAGC ATCACGTTCG CGGCGTGAAA AAACAGAAAA GAGCGGCCGT
    AAAGAAGCAT TGGAACGTTT GAAAAGAGCA AAAGCTGGTG AAAAAGTCAA ATATGAGGTG
    GAAGAAATTT CAAGCATTTA TGAGGAGGTG GATGAAGAAC AATACTCTAA AATTGTTCGT
    GACCGACAGG ATGATGATTG GATAGTGGAT GATGATGGGA CCGGTTATGT AGAAGATGGA
    AGAGAGATTT TTGATGATGA CTTAGAAGAC AATGCTCTTG CTGATTCAGG TAAAAGAGCT
    AAGGGAGCAC CCAAAGATAA ATCTAATGTA AAAAAGTCAT CGGTGTCGAA GCCAAATAAT
    ATCAAGTCAA TGTTTATGGC CAGTGCTGTC AAAAGGACAA CAGATAAAAC TGTGGATCTG
    TCAAAAGATG ACTTGCTTGG TGATTTACTG CAAGATTTAA AGTCACAGGC TCTTCCTATA
    ACACCACCCC CAGTAATTAC TCTAAAGAAG AAGAAACTTG CAGGGTCTCC TTTAAACCCT
    TTCTCGGTAC CACCTGCTGC CCCAAAGGTT TTACCTGTGA AAAAACCACC AGCTTTAGCA
    AAGCTTGGGC ATCTCTCAAG TGCTGCGCCA TCTAACAACT CTGCCAGTTC TTTTGTACCC
    CGGGAAATTA AGAATGAGCC AAATTCTGAT TTGATTTCTT CTGCTCCTTT GAATGTGAAA
    ACTCAAGTAA AAGAAGAGGA TACGTCAATG GTTGAGTTCG ATGATGGAGA TTTTGATGAA
    CCAATGGAAG AAGACAATTT GGAATCTCCT TCAGTTGTTT CTAGCACAAT TAAGAAGGAA
    GAACCAAAAA TTAAGTGTGT GAAAGAGGAT AGTGTTAAAA TGGAGGAAAG TTCTTTCATG
    ACTTCCGCTG CTTTAAATGA ATCTTGTTGG GATCAAATTG ATGAGGTTGA ATCAATGACC
    ACTGAAGTTC AGGTAGATTC AAGCCGCCTA CCCTTGGTTA CAGGTGCAGA TGGAAGTCAG
    GTGTTTAAGT TTTATTGGCT AGATGCCTAT GAAGACCAGT ACAGTCAGCC AGGAGTAGTC
    TACTTATTTG GAAAAGTTTG GATAGAGACA GCTGAGGCGT ATGTCAGCTG CTGTGTTAGT
    GTGAAGAACA TTGAGAGGAC TGTATATCTC CTACCTCGTG AAAATAGAGT GCAGCTTTCT
    ACAGGCAAAG ACACTGGAGT ACCAGTGTCT ATGATGAATG TATACCAGGA GTTTAATGAA
    AGTGTTGCAG AAAAGTACAA AATCATGAAG TTCAAGTCTA AGAAAGTTGA GAAGGATTAT
    GCTTTTGAAA TTCCTGATGT TCCAGCCAGC TCTGAATATC TAGAGGTTCG ATATTCTGCG
    GACTCACCTC AACTTCCTCA GGATCTAAAA GGAGAAACAT TTTCACATGT GTTTGGAACA
    AATACTTCAA GCCTGGAGCT TTTCTTGCTT AGCAGAAAAA TAAAAGGGCC CAGCTGGCTG
    GAGATAAAAA GCCCACAGCT GAGCAGTCAG CCCATGAGCT GGTGCAAGGT TGAGGCAGTG
    GTGATGAGGC CTGACCAAGT GAATGTTGTG AAGGATCTGG CACCTCCACC TGTTGTTGTT
    TTGTCGCTCA GTATGAAGAC TGTGCAGAAT GCAAAGACTC ACCAAAATGA GATTGTGGCA
    ATGGCTGCTT TGGTTCATCA CAAATTTCCC TTTGATAAAG CACCACCACA GCCACCATTC
    CAGACACATT TTTGTGTTCT GAGCAAACCT AATGATTGCA TTTTCCCATA CGACTTTAAG
    GAAGCGGTGA AACGAAAGAA TGCAAATATT GAGATTGCGT TAACTGAACG AACTCTACTG
    GGCTTTTTCC TAGCAAAAAT TCATAAAATT GATCCAGATA TTATTGTAGG CCATGACATT
    TATGGTTTTG ATCTCGAAGT GTTGCTTCAA AGAATTAGCT CGTGCAAGGT TCCTTTCTGG
    TCCAAAATCG GTAGACTGAG AAGATCTGTT ATGCCAAAAC TTGGGGGTCG AAGTGGCTTT
    GCAGAAAGGA ATGCAGCTTG TGGTCGCATC ATTTGCGATA CTGAGATTTC TGCCAAGGAG
    TTAATTCGAT GTAAAAGCTA TCATTTGTCA GAACTGGTAC ATCAAATATT GAAAACAGAG
    AGAGTTTTGA TGCCACCAGA AAACATCCGC AATGCATACA GTGATTCAGC ACATCTTCTT
    TACATGCTGG AGAACACCTG GACTGATGCA AAATTCATCC TTCAGATCAT GTGTGAGCTG
    AATGTTCTTC CCCTGGCCTT GCAGATCACA AATATTGCAG GCAATGTCTT GTCAAGGACA
    TTAATGGGTG GACGCTCAGA AAGAAACGAG TACCTTCTCC TTCATGCCTT CACAGAAAAT
    AACTTCATAG TGCCAGACAA GCCAGTGTTC AAGAAAATTC AACAGACCAC TGTGGAGGAC
    GATGATAATA TGGACACAGA TCAAAATAAA AATAAGAAAG GTCGAAAGAA GGCTGCCTAT
    GCTGGAGGAT TGGTCCTGGA ACCCAAAGTT GGTTTCTACG ACAAATTCAT CTTGCTTCTG
    GATTTCAACA GCTTGTACCC CTCTATCATT CAGGAATATA ATATCTGCTT TACAACAGTG
    CAAAGAGAGG CTCCTACTTC ACAGAAAGGT GAAGATCAGG AAGAAATTCC AGAGCTACCT
    CATTCTGATC TTGAAATGGG GATCTTACCA CGAGAGATAC GGAAACTGGT GGAGAGGAGG
    CGTCACGTGA AGCAGTTAAT GAAGCAGCCT GACCTAAACC CTGATTTATA CTTACAGTAT
    GACATCAGAC AAAAAGCTTT AAAGCTCACG GCAAACAGCA TGTATGGATG CCTGGGCTTC
    TCCTACAGCA GGTTCTATGC CAAACCTTTG GCTGCATTGG TTACTCACCA AGGGAGAGAG
    ATTTTGCTGC ATACCAAGGA AATGGTACAG AAGATGAATC TGGAAGTCAT ATATGGAGAC
    ACGGATTCTA TCATGATAAA CACCAATTGT AATAATTTAG AAGAAGTCTT CAAGCTTGGA
    AACCGGGTGA AAAGTGAGGT AAACAAATCA TACAAACTTT TAGAGATTGA CATTGATGGT
    ATCTTTAAAT CACTCTTGCT ACTTAAGAAA AAGAAGTATG CCGCTCTGAC TGTGGAGCCT
    ACAGGTGATG GAAAGTATGT TACTAAGCAG GAGCTTAAAG GTTTGGATAT TGTCAGAAGA
    GACTGGTGTG AACTTGCTAA AGAAGCTGGA AATTATGTTA TCAGTCAGAT TCTGTCTGAT
    CAGCCACGTG ACAATATTGT TGAGAACATT CAGAAGAAAT TAACTGAAAT TGGGGAGAAT
    GTAACAAATG GAACTGTGCC CATTAGCCAG TATGAAATAA ACAAGGCATT GACAAAGGAT
    CCTCAGGATT ACCCTGATAA AAAAAGCTTA CCTCACGTAC ATGTTGCCAT GTGGATAAAT
    TCCCAAGGTG GAAGAAAGGT GAAAGCTGGT GATACAATAT CCTATGTCAT TTGCCAGGAC
    GGATCAAATC TTAGTGCCAG CCAGAGAGCA TATGCTCCAG AGCAGCTGCA AAAACAAGAG
    AACCTTTCCA TTGACACACA ATACTACCTG TCACAGCAAG TCCACCCAGT AGTGGCTCGG
    ATCTGTGAGC CCATTGATGG AATTGATTCT GCACTCATTG CAATGTGGTT AGGACTTGAC
    CCCTCCCAGT TCCGGGCTCA CCGCCACCAT CAGCAGGATG AGGAAAATGA TGCATTGTTG
    GGAGGTCCTT CCCAACTCAC AGATGAGGAA AAATACAGAG ATTGTGAACG ATTCAAATTC
    TTCTGCCCAA AGTGTGGAAC AGAGAACATT TATGACAATG TTTTTGATGG CTCTGGGCTG
    CAGATTGAAC CCGGCTTGAA ACGCTGCAGT AAACCAGAGT GTGATGCTTC TCCCTTGGAC
    TACGTTATTC AGGTTCAAAA CAAGCTGCTC TTGGACATAA GACGTTACAT AAAGAAATAC
    TACAGTATTC AATTGCTTTG GAGACCACAT GACAAGTCAC TTAACTCCTT GAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017946959.1
    CDS50..4186
    Translation

    Target ORF information:

    RefSeq Version XM_018091470.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis polymerase (DNA directed), alpha 1, catalytic subunit (pola1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091470.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGCACTTGG TGTTCTGTGC GCCCCGCAGT ACCGTCTTCC CTCCTTACCT TGACATGTCA 
    GAATCTGGAA GTTTTGCAGC ATCACGTTCG CGGCGTGAAA AAACAGAAAA GAGCGGCCGT
    AAAGAAGCAT TGGAACGTTT GAAAAGAGCA AAAGCTGGTG AAAAAGTCAA ATATGAGGTG
    GAAGAAATTT CAAGCATTTA TGAGGAGGTG GATGAAGAAC AATACTCTAA AATTGTTCGT
    GACCGACAGG ATGATGATTG GATAGTGGAT GATGATGGGA CCGGTTATGT AGAAGATGGA
    AGAGAGATTT TTGATGATGA CTTAGAAGAC AATGCTCTTG CTGATTCAGG TAAAAGAGCT
    AAGGGAGCAC CCAAAGATAA ATCTAATGTA AAAAAGTCAT CGGTGTCGAA GCCAAATAAT
    ATCAAGTCAA TGTTTATGGC CAGTGCTGTC AAAAGGACAA CAGATAAAAC TGTGGATCTG
    TCAAAAGATG ACTTGCTTGG TGATTTACTG CAAGATTTAA AGTCACAGGC TCTTCCTATA
    ACACCACCCC CAGTAATTAC TCTAAAGAAG AAGAAACTTG CAGGGTCTCC TTTAAACCCT
    TTCTCGGTAC CACCTGCTGC CCCAAAGGTT TTACCTGTGA AAAAACCACC AGCTTTAGCA
    AAGCTTGGGC ATCTCTCAAG TGCTGCGCCA TCTAACAACT CTGCCAGTTC TTTTGTACCC
    CGGGAAATTA AGAATGAGCC AAATTCTGAT TTGATTTCTT CTGCTCCTTT GAATGTGAAA
    ACTCAAGTAA AAGAAGAGGA TACGTCAATG GTTGAGTTCG ATGATGGAGA TTTTGATGAA
    CCAATGGAAG AAGACAATTT GGAATCTCCT TCAGTTGTTT CTAGCACAAT TAAGAAGGAA
    GAACCAAAAA TTAAGTGTGT GAAAGAGGAT AGTGTTAAAA TGGAGGAAAG TTCTTTCATG
    ACTTCCGCTG CTTTAAATGA ATCTTGTTGG GATCAAATTG ATGAGGTTGA ATCAATGACC
    ACTGAAGTTC AGGTAGATTC AAGCCGCCTA CCCTTGGTTA CAGGTGCAGA TGGAAGTCAG
    GTGTTTAAGT TTTATTGGCT AGATGCCTAT GAAGACCAGT ACAGTCAGCC AGGAGTAGTC
    TACTTATTTG GAAAAGTTTG GATAGAGACA GCTGAGGCGT ATGTCAGCTG CTGTGTTAGT
    GTGAAGAACA TTGAGAGGAC TGTATATCTC CTACCTCGTG AAAATAGAGT GCAGCTTTCT
    ACAGGCAAAG ACACTGGAGT ACCAGTGTCT ATGATGAATG TATACCAGGA GTTTAATGAA
    AGTGTTGCAG AAAAGTACAA AATCATGAAG TTCAAGTCTA AGAAAGTTGA GAAGGATTAT
    GCTTTTGAAA TTCCTGATGT TCCAGCCAGC TCTGAATATC TAGAGGTTCG ATATTCTGCG
    GACTCACCTC AACTTCCTCA GGATCTAAAA GGAGAAACAT TTTCACATGT GTTTGGAACA
    AATACTTCAA GCCTGGAGCT TTTCTTGCTT AGCAGAAAAA TAAAAGGGCC CAGCTGGCTG
    GAGATAAAAA GCCCACAGCT GAGCAGTCAG CCCATGAGCT GGTGCAAGGT TGAGGCAGTG
    GTGATGAGGC CTGACCAAGT GAATGTTGTG AAGGATCTGG CACCTCCACC TGTTGTTGTT
    TTGTCGCTCA GTATGAAGAC TGTGCAGAAT GCAAAGACTC ACCAAAATGA GATTGTGGCA
    ATGGCTGCTT TGGTTCATCA CAAATTTCCC TTTGATAAAG CACCACCACA GCCACCATTC
    CAGACACATT TTTGTGTTCT GAGCAAACCT AATGATTGCA TTTTCCCATA CGACTTTAAG
    GAAGCGGTGA AACGAAAGAA TGCAAATATT GAGATTGCGT TAACTGAACG AACTCTACTG
    GGCTTTTTCC TAGCAAAAAT TCATAAAATT GATCCAGATA TTATTGTAGG CCATGACATT
    TATGGTTTTG ATCTCGAAGT GTTGCTTCAA AGAATTAGCT CGTGCAAGGT TCCTTTCTGG
    TCCAAAATCG GTAGACTGAG AAGATCTGTT ATGCCAAAAC TTGGGGGTCG AAGTGGCTTT
    GCAGAAAGGA ATGCAGCTTG TGGTCGCATC ATTTGCGATA CTGAGATTTC TGCCAAGGAG
    TTAATTCGAT GTAAAAGCTA TCATTTGTCA GAACTGGTAC ATCAAATATT GAAAACAGAG
    AGAGTTTTGA TGCCACCAGA AAACATCCGC AATGCATACA GTGATTCAGC ACATCTTCTT
    TACATGCTGG AGAACACCTG GACTGATGCA AAATTCATCC TTCAGATCAT GTGTGAGCTG
    AATGTTCTTC CCCTGGCCTT GCAGATCACA AATATTGCAG GCAATGTCTT GTCAAGGACA
    TTAATGGGTG GACGCTCAGA AAGAAACGAG TACCTTCTCC TTCATGCCTT CACAGAAAAT
    AACTTCATAG TGCCAGACAA GCCAGTGTTC AAGAAAATTC AACAGACCAC TGTGGAGGAC
    GATGATAATA TGGACACAGA TCAAAATAAA AATAAGAAAG GTCGAAAGAA GGCTGCCTAT
    GCTGGAGGAT TGGTCCTGGA ACCCAAAGTT GGTTTCTACG ACAAATTCAT CTTGCTTCTG
    GATTTCAACA GCTTGTACCC CTCTATCATT CAGGAATATA ATATCTGCTT TACAACAGTG
    CAAAGAGAGG CTCCTACTTC ACAGAAAGGT GAAGATCAGG AAGAAATTCC AGAGCTACCT
    CATTCTGATC TTGAAATGGG GATCTTACCA CGAGAGATAC GGAAACTGGT GGAGAGGAGG
    CGTCACGTGA AGCAGTTAAT GAAGCAGCCT GACCTAAACC CTGATTTATA CTTACAGTAT
    GACATCAGAC AAAAAGCTTT AAAGCTCACG GCAAACAGCA TGTATGGATG CCTGGGCTTC
    TCCTACAGCA GGTTCTATGC CAAACCTTTG GCTGCATTGG TTACTCACCA AGGGAGAGAG
    ATTTTGCTGC ATACCAAGGA AATGGTACAG AAGATGAATC TGGAAGTCAT ATATGGAGAC
    ACGGATTCTA TCATGATAAA CACCAATTGT AATAATTTAG AAGAAGTCTT CAAGCTTGGA
    AACCGGGTGA AAAGTGAGGT AAACAAATCA TACAAACTTT TAGAGATTGA CATTGATGGT
    ATCTTTAAAT CACTCTTGCT ACTTAAGAAA AAGAAGTATG CCGCTCTGAC TGTGGAGCCT
    ACAGGTGATG GAAAGTATGT TACTAAGCAG GAGCTTAAAG GTTTGGATAT TGTCAGAAGA
    GACTGGTGTG AACTTGCTAA AGAAGCTGGA AATTATGTTA TCAGTCAGAT TCTGTCTGAT
    CAGCCACGTG ACAATATTGT TGAGAACATT CAGAAGAAAT TAACTGAAAT TGGGGAGAAT
    GTAACAAATG GAACTGTGCC CATTAGCCAG TATGAAATAA ACAAGGCATT GACAAAGGAT
    CCTCAGGATT ACCCTGATAA AAAAAGCTTA CCTCACGTAC ATGTTGCCAT GTGGATAAAT
    TCCCAAGGTG GAAGAAAGGT GAAAGCTGGT GATACAATAT CCTATGTCAT TTGCCAGGAC
    GGATCAAATC TTAGTGCCAG CCAGAGAGCA TATGCTCCAG AGCAGCTGCA AAAACAAGAG
    AACCTTTCCA TTGACACACA ATACTACCTG TCACAGCAAG TCCACCCAGT AGTGGCTCGG
    ATCTGTGAGC CCATTGATGG AATTGATTCT GCACTCATTG CAATGTGGTT AGGACTTGAC
    CCCTCCCAGT TCCGGGCTCA CCGCCACCAT CAGCAGGATG AGGAAAATGA TGCATTGTTG
    GGAGGTCCTT CCCAACTCAC AGATGAGGAA AAATACAGAG ATTGTGAACG ATTCAAATTC
    TTCTGCCCAA AGTGTGGAAC AGAGAACATT TATGACAATG TTTTTGATGG CTCTGGGCTG
    CAGATTGAAC CCGGCTTGAA ACGCTGCAGT AAACCAGAGT GTGATGCTTC TCCCTTGGAC
    TACGTTATTC AGGTTCAAAA CAAGCTGCTC TTGGACATAA GACGTTACAT AAAGAAATAC
    TACAGTATTC AATTGCTTTG GAGACCACAT GACAAGTCAC TTAACTCCTT GAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52095
    Clone ID Related Accession (Same CDS sequence) XM_031896702.1
    Accession Version XM_031896702.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4470bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA polymerase alpha catalytic subunit isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCACTTGG TGTTCTGTGC GCCCCGCAGT ACCGTCTTCC CTCCTTACCT TGACATGTCA 
    GAATCTGGAA GTTTTGCAGC ATCACGTTCG CGGCGTGAAA AAACAGAAAA GAGCGGCCGT
    AAAGAAGCAT TGGAACGTTT GAAAAGAGCA AAAGCTGGTG AAAAAGTCAA ATATGAGGTG
    GAAGAAATTT CAAGCATTTA TGAGGAGGTG GATGAAGAAC AATACTCTAA AATTGTTCGT
    GACCGACAGG ATGATGATTG GATAGTGGAT GATGATGGGA CCGGTTATGT AGAAGATGGA
    AGAGAGATTT TTGATGATGA CTTAGAAGAC AATGCTCTTG CTGATTCAGG TAAAAGAGCT
    AAGGGAGCAC CCAAAGATAA ATCTAATGTA AAAAAGTCAT CGGTGTCGAA GCCAAATAAT
    ATCAAGTCAA TGTTTATGGC CAGTGCTGTC AAAAGGACAA CAGATAAAAC TGTGGATCTG
    TCAAAAGATG ACTTGCTTGG TGATTTACTG CAAGATTTAA AGTCACAGGC TCTTCCTATA
    ACACCACCCC CAGTAATTAC TCTAAAGAAG AAGAAACTTG CAGGGTCTCC TTTAAACCCT
    TTCTCGGTAC CACCTGCTGC CCCAAAGGTT TTACCTGTGA AAAAACCACC AGCTTTAGCA
    AAGCTTGGGC ATCTCTCAAG TGCTGCGCCA TCTAACAACT CTGCCAGTTC TTTTGTACCC
    CGGGAAATTA AGAATGAGCC AAATTCTGAT TTGATTTCTT CTGCTCCTTT GAATGTGAAA
    ACTCAAGTAA AAGAAGAGGA TACGTCAATG GTTGAGTTCG ATGATGGAGA TTTTGATGAA
    CCAATGGAAG AAGACAATTT GGAATCTCCT TCAGTTGTTT CTAGCACAAT TAAGAAGGAA
    GAACCAAAAA TTAAGTGTGT GAAAGAGGAT AGTGTTAAAA TGGAGGAAAG TTCTTTCATG
    ACTTCCGCTG CTTTAAATGA ATCTTGTTGG GATCAAATTG ATGAGGTTGA ATCAATGACC
    ACTGAAGTTC AGGTAGATTC AAGCCGCCTA CCCTTGGTTA CAGGTGCAGA TGGAAGTCAG
    GTGTTTAAGT TTTATTGGCT AGATGCCTAT GAAGACCAGT ACAGTCAGCC AGGAGTAGTC
    TACTTATTTG GAAAAGTTTG GATAGAGACA GCTGAGGCGT ATGTCAGCTG CTGTGTTAGT
    GTGAAGAACA TTGAGAGGAC TGTATATCTC CTACCTCGTG AAAATAGAGT GCAGCTTTCT
    ACAGGCAAAG ACACTGGAGT ACCAGTGTCT ATGATGAATG TATACCAGGA GTTTAATGAA
    AGTGTTGCAG AAAAGTACAA AATCATGAAG TTCAAGTCTA AGAAAGTTGA GAAGGATTAT
    GCTTTTGAAA TTCCTGATGT TCCAGCCAGC TCTGAATATC TAGAGGTTCG ATATTCTGCG
    GACTCACCTC AACTTCCTCA GGATCTAAAA GGAGAAACAT TTTCACATGT GTTTGGAACA
    AATACTTCAA GCCTGGAGCT TTTCTTGCTT AGCAGAAAAA TAAAAGGGCC CAGCTGGCTG
    GAGATAAAAA GCCCACAGCT GAGCAGTCAG CCCATGAGCT GGTGCAAGGT TGAGGCAGTG
    GTGATGAGGC CTGACCAAGT GAATGTTGTG AAGGATCTGG CACCTCCACC TGTTGTTGTT
    TTGTCGCTCA GTATGAAGAC TGTGCAGAAT GCAAAGACTC ACCAAAATGA GATTGTGGCA
    ATGGCTGCTT TGGTTCATCA CAAATTTCCC CTTGATAAAG CACCACCACA GCCACCATTC
    CAGACACATT TTTGTGTTCT GAGCAAACCT AATGATTGCA TTTTCCCATA CGACTTTAAG
    GAAGCGGTGA AACGAAAGAA TGCAAATATT GAGATTGCGT TAACTGAACG AACTCTACTG
    GGCTTTTTCC TAGCAAAAAT TCATAAAATT GATCCAGATA TTATTGTAGG CCATGACATT
    TATGGTTTTG ATCTCGAAGT GTTGCTTCAA AGAATTAGCT CGTGCAAGGT TCCTTTCTGG
    TCCAAAATCG GTAGACTGAG AAGATCTGTT ATGCCAAAAC TTGGGGGTCG AAGTGGCTTT
    GCAGAAAGGA ATGCAGCTTG TGGTCGCATC ATTTGCGATA CTGAGATTTC TGCCAAGGAG
    TTAATTCGAT GTAAAAGCTA TCATTTGTCA GAACTGGTAC ATCAAATATT GAAAACAGAG
    AGAGTTTTGA TGCCACCAGA AAACATCCGC AATGCATACA GTGATTCAGC ACATCTTCTT
    TACATGCTGG AGAACACCTG GACTGATGCA AAATTCATCC TTCAGATCAT GTGTGAGCTG
    AATGTTCTTC CCCTGGCCTT GCAGATCACA AATATTGCAG GCAATGTCTT GTCAAGGACA
    TTAATGGGTG GACGCTCAGA AAGAAACGAG TACCTTCTCC TTCATGCCTT CACAGAAAAT
    AACTTCATAG TGCCAGACAA GCCAGTGTTC AAGAAAATTC AACAGACCAC TGTGGAGGAC
    GATGATAATA TGGACACAGA TCAAAATAAA AATAAGAAAG GTCGAAAGAA GGCTGCCTAT
    GCTGGAGGAT TGGTCCTGGA ACCCAAAGTT GGTTTCTACG ACAAATTCAT CTTGCTTCTG
    GATTTCAACA GCTTGTACCC CTCTATCATT CAGGAATATA ATATCTGCTT TACAACAGTG
    CAAAGAGAGG CTCCTACTTC ACAGAAAGGT GAAGATCAGG AAGAAATTCC AGAGCTACCT
    CATTCTGATC TTGAAATGGG GATCTTACCA CGAGAGATAC GGAAACTGGT GGAGAGGAGG
    CGTCACGTGA AGCAGTTAAT GAAGCAGCCT GACCTAAACC CTGATTTATA CTTACAGTAT
    GACATCAGAC AAAAAGCTTT AAAGCTCACG GCAAACAGCA TGTATGGATG CCTGGGCTTC
    TCCTACAGCA GGTTCTATGC CAAACCTTTG GCTGCATTGG TTACTCACCA AGGGAGAGAG
    ATTTTGCTGC ATACCAAGGA AATGGTACAG AAGATGAATC TGGAAGTCAT ATATGGAGAC
    ACGGATTCTA TCATGATAAA CACCAATTGT AATAATTTAG AAGAAGTCTT CAAGCTTGGA
    AACCGGGTGA AAAGTGAGGT AAACAAATCA TACAAACTTT TAGAGATTGA CATTGATGGT
    ATCTTTAAAT CACTCTTGCT ACTTAAGAAA AAGAAGTATG CCGCTCTGAC TGTGGAGCCT
    ACAGGTGATG GAAAGTATGT TACTAAGCAG GAGCTTAAAG GTTTGGATAT TGTCAGAAGA
    GACTGGTGTG AACTTGCTAA AGAAGCTGGA AATTATGTTA TCAGTCAGAT TCTGTCTGAT
    CAGCCACGTG ACAATATTGT TGAGAACATT CAGAAGAAAT TAACTGAAAT TGGGGAGAAT
    GTAACAAATG GAACTGTGCC CATTAGCCAG TATGAAATAA ACAAGGCATT GACAAAGGAT
    CCTCAGGATT ACCCTGATAA AAAAAGCTTA CCTCACGTAC ATGTTGCCAT GTGGATAAAT
    TCCCAAGGTG GAAGAAAGGT GAAAGCTGGT GATACAATAT CCTATGTCAT TTGCCAGGAC
    GGATCAAATC TTAGTGCCAG CCAGAGAGCA TATGCTCCAG AGCAGCTGCA AAAACAAGAG
    AACCTTTCCA TTGACACACA ATACTACCTG TCACAGCAAG TCCACCCAGT AGTGGCTCGG
    ATCTGTGAGC CCATTGATGG AATTGATTCT GCACTCATTG CAATGTGGTT AGGACTTGAC
    CCCTCCCAGT TCCGGGCTCA CCGCCACCAT CAGCAGGATG AGGAAAATGA TGCATTGTTG
    GGAGGTCCTT CCCAACTCAC AGATGAGGAA AAATACAGAG ATTGTGAACG ATTCAAATTC
    TTCTGCCCAA AGTGTGGAAC AGAGAACATT TATGACAATG TTTTTGATGG CTCTGGGCTG
    CAGATTGAAC CCGGCTTGAA ACGCTGCAGT AAACCAGAGT GTGATGCTTC TCCCTTGGAC
    TACGTTATTC AGGTTCAAAA CAAGCTGCTC TTGGACATAA GACGTTACAT AAAGAAATAC
    TACAGTGGTT GGCTTGTATG TGAAGAAAAA ACCTGCCAGA ACAGAACACG ACGAGTTCCT
    CTGAGCTTCT CCCGCAATGG GCCGATATGC CAAGCTTGCA CTAAAGCAAC GCTAAGATCA
    GAGTATCCGG AAAAGGCCCT GTACACTCAG CTTTGTTTCT ATAGATTCAT TTTTGACTGG
    GATTATGCAC TGGAGAAAGT TGTTTCTGAA CAAGAGAGAG TTATGGAAGA TGATCCTGGA
    TGTTTATGCA ACTTACTGGT TGGTCCTGAA ATATCAATCT TTTCTGGCGC TCTTATGATA
    AGAATGACTT TGGAAAAAAC ATTACTGACT CAAATAGTGA GCCTTAGACA TGCGCTGCAT
    TGCTTTTTCT GCACCTTTAC AAATCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752562.1
    CDS52..4521
    Translation

    Target ORF information:

    RefSeq Version XM_031896702.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis polymerase (DNA directed), alpha 1, catalytic subunit (pola1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896702.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCACTTGG TGTTCTGTGC GCCCCGCAGT ACCGTCTTCC CTCCTTACCT TGACATGTCA 
    GAATCTGGAA GTTTTGCAGC ATCACGTTCG CGGCGTGAAA AAACAGAAAA GAGCGGCCGT
    AAAGAAGCAT TGGAACGTTT GAAAAGAGCA AAAGCTGGTG AAAAAGTCAA ATATGAGGTG
    GAAGAAATTT CAAGCATTTA TGAGGAGGTG GATGAAGAAC AATACTCTAA AATTGTTCGT
    GACCGACAGG ATGATGATTG GATAGTGGAT GATGATGGGA CCGGTTATGT AGAAGATGGA
    AGAGAGATTT TTGATGATGA CTTAGAAGAC AATGCTCTTG CTGATTCAGG TAAAAGAGCT
    AAGGGAGCAC CCAAAGATAA ATCTAATGTA AAAAAGTCAT CGGTGTCGAA GCCAAATAAT
    ATCAAGTCAA TGTTTATGGC CAGTGCTGTC AAAAGGACAA CAGATAAAAC TGTGGATCTG
    TCAAAAGATG ACTTGCTTGG TGATTTACTG CAAGATTTAA AGTCACAGGC TCTTCCTATA
    ACACCACCCC CAGTAATTAC TCTAAAGAAG AAGAAACTTG CAGGGTCTCC TTTAAACCCT
    TTCTCGGTAC CACCTGCTGC CCCAAAGGTT TTACCTGTGA AAAAACCACC AGCTTTAGCA
    AAGCTTGGGC ATCTCTCAAG TGCTGCGCCA TCTAACAACT CTGCCAGTTC TTTTGTACCC
    CGGGAAATTA AGAATGAGCC AAATTCTGAT TTGATTTCTT CTGCTCCTTT GAATGTGAAA
    ACTCAAGTAA AAGAAGAGGA TACGTCAATG GTTGAGTTCG ATGATGGAGA TTTTGATGAA
    CCAATGGAAG AAGACAATTT GGAATCTCCT TCAGTTGTTT CTAGCACAAT TAAGAAGGAA
    GAACCAAAAA TTAAGTGTGT GAAAGAGGAT AGTGTTAAAA TGGAGGAAAG TTCTTTCATG
    ACTTCCGCTG CTTTAAATGA ATCTTGTTGG GATCAAATTG ATGAGGTTGA ATCAATGACC
    ACTGAAGTTC AGGTAGATTC AAGCCGCCTA CCCTTGGTTA CAGGTGCAGA TGGAAGTCAG
    GTGTTTAAGT TTTATTGGCT AGATGCCTAT GAAGACCAGT ACAGTCAGCC AGGAGTAGTC
    TACTTATTTG GAAAAGTTTG GATAGAGACA GCTGAGGCGT ATGTCAGCTG CTGTGTTAGT
    GTGAAGAACA TTGAGAGGAC TGTATATCTC CTACCTCGTG AAAATAGAGT GCAGCTTTCT
    ACAGGCAAAG ACACTGGAGT ACCAGTGTCT ATGATGAATG TATACCAGGA GTTTAATGAA
    AGTGTTGCAG AAAAGTACAA AATCATGAAG TTCAAGTCTA AGAAAGTTGA GAAGGATTAT
    GCTTTTGAAA TTCCTGATGT TCCAGCCAGC TCTGAATATC TAGAGGTTCG ATATTCTGCG
    GACTCACCTC AACTTCCTCA GGATCTAAAA GGAGAAACAT TTTCACATGT GTTTGGAACA
    AATACTTCAA GCCTGGAGCT TTTCTTGCTT AGCAGAAAAA TAAAAGGGCC CAGCTGGCTG
    GAGATAAAAA GCCCACAGCT GAGCAGTCAG CCCATGAGCT GGTGCAAGGT TGAGGCAGTG
    GTGATGAGGC CTGACCAAGT GAATGTTGTG AAGGATCTGG CACCTCCACC TGTTGTTGTT
    TTGTCGCTCA GTATGAAGAC TGTGCAGAAT GCAAAGACTC ACCAAAATGA GATTGTGGCA
    ATGGCTGCTT TGGTTCATCA CAAATTTCCC CTTGATAAAG CACCACCACA GCCACCATTC
    CAGACACATT TTTGTGTTCT GAGCAAACCT AATGATTGCA TTTTCCCATA CGACTTTAAG
    GAAGCGGTGA AACGAAAGAA TGCAAATATT GAGATTGCGT TAACTGAACG AACTCTACTG
    GGCTTTTTCC TAGCAAAAAT TCATAAAATT GATCCAGATA TTATTGTAGG CCATGACATT
    TATGGTTTTG ATCTCGAAGT GTTGCTTCAA AGAATTAGCT CGTGCAAGGT TCCTTTCTGG
    TCCAAAATCG GTAGACTGAG AAGATCTGTT ATGCCAAAAC TTGGGGGTCG AAGTGGCTTT
    GCAGAAAGGA ATGCAGCTTG TGGTCGCATC ATTTGCGATA CTGAGATTTC TGCCAAGGAG
    TTAATTCGAT GTAAAAGCTA TCATTTGTCA GAACTGGTAC ATCAAATATT GAAAACAGAG
    AGAGTTTTGA TGCCACCAGA AAACATCCGC AATGCATACA GTGATTCAGC ACATCTTCTT
    TACATGCTGG AGAACACCTG GACTGATGCA AAATTCATCC TTCAGATCAT GTGTGAGCTG
    AATGTTCTTC CCCTGGCCTT GCAGATCACA AATATTGCAG GCAATGTCTT GTCAAGGACA
    TTAATGGGTG GACGCTCAGA AAGAAACGAG TACCTTCTCC TTCATGCCTT CACAGAAAAT
    AACTTCATAG TGCCAGACAA GCCAGTGTTC AAGAAAATTC AACAGACCAC TGTGGAGGAC
    GATGATAATA TGGACACAGA TCAAAATAAA AATAAGAAAG GTCGAAAGAA GGCTGCCTAT
    GCTGGAGGAT TGGTCCTGGA ACCCAAAGTT GGTTTCTACG ACAAATTCAT CTTGCTTCTG
    GATTTCAACA GCTTGTACCC CTCTATCATT CAGGAATATA ATATCTGCTT TACAACAGTG
    CAAAGAGAGG CTCCTACTTC ACAGAAAGGT GAAGATCAGG AAGAAATTCC AGAGCTACCT
    CATTCTGATC TTGAAATGGG GATCTTACCA CGAGAGATAC GGAAACTGGT GGAGAGGAGG
    CGTCACGTGA AGCAGTTAAT GAAGCAGCCT GACCTAAACC CTGATTTATA CTTACAGTAT
    GACATCAGAC AAAAAGCTTT AAAGCTCACG GCAAACAGCA TGTATGGATG CCTGGGCTTC
    TCCTACAGCA GGTTCTATGC CAAACCTTTG GCTGCATTGG TTACTCACCA AGGGAGAGAG
    ATTTTGCTGC ATACCAAGGA AATGGTACAG AAGATGAATC TGGAAGTCAT ATATGGAGAC
    ACGGATTCTA TCATGATAAA CACCAATTGT AATAATTTAG AAGAAGTCTT CAAGCTTGGA
    AACCGGGTGA AAAGTGAGGT AAACAAATCA TACAAACTTT TAGAGATTGA CATTGATGGT
    ATCTTTAAAT CACTCTTGCT ACTTAAGAAA AAGAAGTATG CCGCTCTGAC TGTGGAGCCT
    ACAGGTGATG GAAAGTATGT TACTAAGCAG GAGCTTAAAG GTTTGGATAT TGTCAGAAGA
    GACTGGTGTG AACTTGCTAA AGAAGCTGGA AATTATGTTA TCAGTCAGAT TCTGTCTGAT
    CAGCCACGTG ACAATATTGT TGAGAACATT CAGAAGAAAT TAACTGAAAT TGGGGAGAAT
    GTAACAAATG GAACTGTGCC CATTAGCCAG TATGAAATAA ACAAGGCATT GACAAAGGAT
    CCTCAGGATT ACCCTGATAA AAAAAGCTTA CCTCACGTAC ATGTTGCCAT GTGGATAAAT
    TCCCAAGGTG GAAGAAAGGT GAAAGCTGGT GATACAATAT CCTATGTCAT TTGCCAGGAC
    GGATCAAATC TTAGTGCCAG CCAGAGAGCA TATGCTCCAG AGCAGCTGCA AAAACAAGAG
    AACCTTTCCA TTGACACACA ATACTACCTG TCACAGCAAG TCCACCCAGT AGTGGCTCGG
    ATCTGTGAGC CCATTGATGG AATTGATTCT GCACTCATTG CAATGTGGTT AGGACTTGAC
    CCCTCCCAGT TCCGGGCTCA CCGCCACCAT CAGCAGGATG AGGAAAATGA TGCATTGTTG
    GGAGGTCCTT CCCAACTCAC AGATGAGGAA AAATACAGAG ATTGTGAACG ATTCAAATTC
    TTCTGCCCAA AGTGTGGAAC AGAGAACATT TATGACAATG TTTTTGATGG CTCTGGGCTG
    CAGATTGAAC CCGGCTTGAA ACGCTGCAGT AAACCAGAGT GTGATGCTTC TCCCTTGGAC
    TACGTTATTC AGGTTCAAAA CAAGCTGCTC TTGGACATAA GACGTTACAT AAAGAAATAC
    TACAGTGGTT GGCTTGTATG TGAAGAAAAA ACCTGCCAGA ACAGAACACG ACGAGTTCCT
    CTGAGCTTCT CCCGCAATGG GCCGATATGC CAAGCTTGCA CTAAAGCAAC GCTAAGATCA
    GAGTATCCGG AAAAGGCCCT GTACACTCAG CTTTGTTTCT ATAGATTCAT TTTTGACTGG
    GATTATGCAC TGGAGAAAGT TGTTTCTGAA CAAGAGAGAG TTATGGAAGA TGATCCTGGA
    TGTTTATGCA ACTTACTGGT TGGTCCTGAA ATATCAATCT TTTCTGGCGC TCTTATGATA
    AGAATGACTT TGGAAAAAAC ATTACTGACT CAAATAGTGA GCCTTAGACA TGCGCTGCAT
    TGCTTTTTCT GCACCTTTAC AAATCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52096
    Clone ID Related Accession (Same CDS sequence) XM_012957632.3
    Accession Version XM_012957632.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4443bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA polymerase alpha catalytic subunit isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957632.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCACTTGG TGTTCTGTGC GCCCCGCAGT ACCGTCTTCC CTCCTTACCT TGACATGTCA 
    GAATCTGGAA GTTTTGCAGC ATCACGTTCG CGGCGTGAAA AAACAGAAAA GAGCGGCCGT
    AAAGAAGCAT TGGAACGTTT GAAAAGAGCA AAAGCTGGTG AAAAAGTCAA ATATGAGGTG
    GAAGAAATTT CAAGCATTTA TGAGGAGGTG GATGAAGAAC AATACTCTAA AATTGTTCGT
    GACCGACAGG ATGATGATTG GATAGTGGAT GATGATGGGA CCGGTTATGT AGAAGATGGA
    AGAGAGATTT TTGATGATGA CTTAGAAGAC AATGCTCTTG CTGATTCAGG TAAAAGAGCT
    AAGGGAGCAC CCAAAGATAA ATCTAATGTA AAAAAGTCAT CGGTGTCGAA GCCAAATAAT
    ATCAAGTCAA TGTTTATGGC CAGTGCTGTC AAAAGGACAA CAGATAAAAC TGTGGATCTG
    TCAAAAGATG ACTTGCTTGG TGATTTACTG CAAGATTTAA AGTCACAGGC TCTTCCTATA
    ACACCACCCC CAGTAATTAC TCTAAAGAAG AAGAAACTTG CAGGGTCTCC TTTAAACCCT
    TTCTCGGTAC CACCTGCTGC CCCAAAGGTT TTACCTGTGA AAAAACCACC AGCTTTAGCA
    AAGCTTGGGC ATCTCTCAAG TGCTGCGCCA TCTAACAACT CTGCCAGTTC TTTTGTACCC
    CGGGAAATTA AGAATGAGCC AAATTCTGAT TTGATTTCTT CTGCTCCTTT GAATGTGAAA
    ACTCAAGTAA AAGAAGAGGA TACGTCAATG GTTGAGTTCG ATGATGGAGA TTTTGATGAA
    CCAATGGAAG AAGACAATTT GGAATCTCCT TCAGTTGTTT CTAGCACAAT TAAGAAGGAA
    GAACCAAAAA TTAAGTGTGT GAAAGAGGAT AGTGTTAAAA TGGAGGAAAG TTCTTTCATG
    ACTTCCGCTG CTTTAAATGA ATCTTGTTGG GATCAAATTG ATGAGGTTGA ATCAATGACC
    ACTGAAGTTC AGGTAGATTC AAGCCGCCTA CCCTTGGTTA CAGGTGCAGA TGGAAGTCAG
    GTGTTTAAGT TTTATTGGCT AGATGCCTAT GAAGACCAGT ACAGTCAGCC AGGAGTAGTC
    TACTTATTTG GAAAAGTTTG GATAGAGACA GCTGAGGCGT ATGTCAGCTG CTGTGTTAGT
    GTGAAGAACA TTGAGAGGAC TGTATATCTC CTACCTCGTG AAAATAGAGT GCAGCTTTCT
    ACAGGCAAAG ACACTGGAGT ACCAGTGTCT ATGATGAATG TATACCAGGA GTTTAATGAA
    AGTGTTGCAG AAAAGTACAA AATCATGAAG TTCAAGTCTA AGAAAGTTGA GAAGGATTAT
    GCTTTTGAAA TTCCTGATGT TCCAGCCAGC TCTGAATATC TAGAGGTTCG ATATTCTGCG
    GACTCACCTC AACTTCCTCA GGATCTAAAA GGAGAAACAT TTTCACATGT GTTTGGAACA
    AATACTTCAA GCCTGGAGCT TTTCTTGCTT AGCAGAAAAA TAAAAGGGCC CAGCTGGCTG
    GAGATAAAAA GCCCACAGCT GAGCAGTCAG CCCATGAGCT GGTGCAAGGT TGAGGCAGTG
    GTGATGAGGC CTGACCAAGT GAATGTTGTG AAGGATCTGG CACCTCCACC TGTTGTTGTT
    TTGTCGCTCA GTATGAAGAC TGTGCAGAAT GCAAAGACTC ACCAAAATGA GATTGTGGCA
    ATGGCTGCTT TGGTTCATCA CAAATTTCCC CTTGATAAAG CACCACCACA GCCACCATTC
    CAGACACATT TTTGTGTTCT GAGCAAACCT AATGATTGCA TTTTCCCATA CGACTTTAAG
    GAAGCGGTGA AACGAAAGAA TGCAAATATT GAGATTGCGT TAACTGAACG AACTCTACTG
    GGCTTTTTCC TAGCAAAAAT TCATAAAATT GATCCAGATA TTATTGTAGG CCATGACATT
    TATGGTTTTG ATCTCGAAGT GTTGCTTCAA AGAATTAGCT CGTGCAAGGT TCCTTTCTGG
    TCCAAAATCG GTAGACTGAG AAGATCTGTT ATGCCAAAAC TTGGGGGTCG AAGTGGCTTT
    GCAGAAAGGA ATGCAGCTTG TGGTCGCATC ATTTGCGATA CTGAGATTTC TGCCAAGGAG
    TTAATTCGAT GTAAAAGCTA TCATTTGTCA GAACTGGTAC ATCAAATATT GAAAACAGAG
    AGAGTTTTGA TGCCACCAGA AAACATCCGC AATGCATACA GTGATTCAGC ACATCTTCTT
    TACATGCTGG AGAACACCTG GACTGATGCA AAATTCATCC TTCAGATCAT GTGTGAGCTG
    AATGTTCTTC CCCTGGCCTT GCAGATCACA AATATTGCAG GCAATGTCTT GTCAAGGACA
    TTAATGGGTG GACGCTCAGA AAGAAACGAG TACCTTCTCC TTCATGCCTT CACAGAAAAT
    AACTTCATAG TGCCAGACAA GCCAGTGTTC AAGAAAATTC AACAGACCAC TGTGGAGGAC
    GATGATAATA TGGACACAGA TCAAAATAAA AATAAGAAAG GTCGAAAGAA GGCTGCCTAT
    GCTGGAGGAT TGGTCCTGGA ACCCAAAGTT GGTTTCTACG ACAAATTCAT CTTGCTTCTG
    GATTTCAACA GCTTGTACCC CTCTATCATT CAGGAATATA ATATCTGCTT TACAACAGTG
    CAAAGAGAGG CTCCTACTTC ACAGAAAGGT GAAGATCAGG AAGAAATTCC AGAGCTACCT
    CATTCTGATC TTGAAATGGG GATCTTACCA CGAGAGATAC GGAAACTGGT GGAGAGGAGG
    CGTCACGTGA AGCAGTTAAT GAAGCAGCCT GACCTAAACC CTGATTTATA CTTACAGTAT
    GACATCAGAC AAAAAGCTTT AAAGCTCACG GCAAACAGCA TGTATGGATG CCTGGGCTTC
    TCCTACAGCA GGTTCTATGC CAAACCTTTG GCTGCATTGG TTACTCACCA AGGGAGAGAG
    ATTTTGCTGC ATACCAAGGA AATGGTACAG AAGATGAATC TGGAAGTCAT ATATGGAGAC
    ACGGATTCTA TCATGATAAA CACCAATTGT AATAATTTAG AAGAAGTCTT CAAGCTTGGA
    AACCGGGTGA AAAGTGAGGT AAACAAATCA TACAAACTTT TAGAGATTGA CATTGATGGT
    ATCTTTAAAT CACTCTTGCT ACTTAAGAAA AAGAAGTATG CCGCTCTGAC TGTGGAGCCT
    ACAGGTGATG GAAAGTATGT TACTAAGCAG GAGCTTAAAG GTTTGGATAT TGTCAGAAGA
    GACTGGTGTG AACTTGCTAA AGAAGCTGGA AATTATGTTA TCAGTCAGAT TCTGTCTGAT
    CAGCCACGTG ACAATATTGT TGAGAACATT CAGAAGAAAT TAACTGAAAT TGGGGAGAAT
    GTAACAAATG GAACTGTGCC CATTAGCCAG TATGAAATAA ACAAGGCATT GACAAAGGAT
    CCTCAGGATT ACCCTGATAA AAAAAGCTTA CCTCACGTAC ATGTTGCCAT GTGGATAAAT
    TCCCAAGGTG GAAGAAAGGT GAAAGCTGGT GATACAATAT CCTATGTCAT TTGCCAGGAC
    GGATCAAATC TTAGTGCCAG CCAGAGAGCA TATGCTCCAG AGCAGCTGCA AAAACAAGAG
    AACCTTTCCA TTGACACACA ATACTACCTG TCACAGCAAG TCCACCCAGT AGTGGCTCGG
    ATCTGTGAGC CCATTGATGG AATTGATTCT GCACTCATTG CAATGTGGTT AGGACTTGAC
    CCCTCCCAGT TCCGGGCTCA CCGCCACCAT CAGCAGGATG AGGAAAATGA TGCATTGTTG
    GGAGGTCCTT CCCAACTCAC AGATGAGGAA AAATACAGAG ATTGTGAACG ATTCAAATTC
    TTCTGCCCAA AGTGTGGAAC AGAGAACATT TATGACAATG TTTTTGATGG CTCTGGGCTG
    CAGATTGAAC CCGGCTTGAA ACGCTGCAGT AAACCAGAGT GTGATGCTTC TCCCTTGGAC
    TACGTTATTC AGGTTCAAAA CAAGCTGCTC TTGGACATAA GACGTTACAT AAAGAAATAC
    TACAGTGGTT GGCTTGTATG TGAAGAAAAA ACCTGCCAGA ACAGAACACG ACGAGTTCCT
    CTGAGCTTCT CCCGCAATGG GCCGATATGC CAAGCTTGCA CTAAAGCAAC GCTAAGATCA
    GAGTATCCGG AAAAGGCCCT GTACACTCAG CTTTGTTTCT ATAGATTCAT TTTTGACTGG
    GATTATGCAC TGGAGAAAGT TGTTTCTGAA CAAGAGAGAG GTCACTTAAA AAAGAAGCAT
    TTTCAAGAGT CAGAAAATCT GTACAAGAAG TTAAAGAGCA CTGTCGACCA AGTACTTTCA
    CAGAGCGGGT ATTCAGAAGT GAACCTCAGC AAGCTGTTCC AAACACTCAG CACCATTAAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813086.2
    CDS50..4492
    Translation

    Target ORF information:

    RefSeq Version XM_012957632.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis polymerase (DNA directed), alpha 1, catalytic subunit (pola1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957632.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCACTTGG TGTTCTGTGC GCCCCGCAGT ACCGTCTTCC CTCCTTACCT TGACATGTCA 
    GAATCTGGAA GTTTTGCAGC ATCACGTTCG CGGCGTGAAA AAACAGAAAA GAGCGGCCGT
    AAAGAAGCAT TGGAACGTTT GAAAAGAGCA AAAGCTGGTG AAAAAGTCAA ATATGAGGTG
    GAAGAAATTT CAAGCATTTA TGAGGAGGTG GATGAAGAAC AATACTCTAA AATTGTTCGT
    GACCGACAGG ATGATGATTG GATAGTGGAT GATGATGGGA CCGGTTATGT AGAAGATGGA
    AGAGAGATTT TTGATGATGA CTTAGAAGAC AATGCTCTTG CTGATTCAGG TAAAAGAGCT
    AAGGGAGCAC CCAAAGATAA ATCTAATGTA AAAAAGTCAT CGGTGTCGAA GCCAAATAAT
    ATCAAGTCAA TGTTTATGGC CAGTGCTGTC AAAAGGACAA CAGATAAAAC TGTGGATCTG
    TCAAAAGATG ACTTGCTTGG TGATTTACTG CAAGATTTAA AGTCACAGGC TCTTCCTATA
    ACACCACCCC CAGTAATTAC TCTAAAGAAG AAGAAACTTG CAGGGTCTCC TTTAAACCCT
    TTCTCGGTAC CACCTGCTGC CCCAAAGGTT TTACCTGTGA AAAAACCACC AGCTTTAGCA
    AAGCTTGGGC ATCTCTCAAG TGCTGCGCCA TCTAACAACT CTGCCAGTTC TTTTGTACCC
    CGGGAAATTA AGAATGAGCC AAATTCTGAT TTGATTTCTT CTGCTCCTTT GAATGTGAAA
    ACTCAAGTAA AAGAAGAGGA TACGTCAATG GTTGAGTTCG ATGATGGAGA TTTTGATGAA
    CCAATGGAAG AAGACAATTT GGAATCTCCT TCAGTTGTTT CTAGCACAAT TAAGAAGGAA
    GAACCAAAAA TTAAGTGTGT GAAAGAGGAT AGTGTTAAAA TGGAGGAAAG TTCTTTCATG
    ACTTCCGCTG CTTTAAATGA ATCTTGTTGG GATCAAATTG ATGAGGTTGA ATCAATGACC
    ACTGAAGTTC AGGTAGATTC AAGCCGCCTA CCCTTGGTTA CAGGTGCAGA TGGAAGTCAG
    GTGTTTAAGT TTTATTGGCT AGATGCCTAT GAAGACCAGT ACAGTCAGCC AGGAGTAGTC
    TACTTATTTG GAAAAGTTTG GATAGAGACA GCTGAGGCGT ATGTCAGCTG CTGTGTTAGT
    GTGAAGAACA TTGAGAGGAC TGTATATCTC CTACCTCGTG AAAATAGAGT GCAGCTTTCT
    ACAGGCAAAG ACACTGGAGT ACCAGTGTCT ATGATGAATG TATACCAGGA GTTTAATGAA
    AGTGTTGCAG AAAAGTACAA AATCATGAAG TTCAAGTCTA AGAAAGTTGA GAAGGATTAT
    GCTTTTGAAA TTCCTGATGT TCCAGCCAGC TCTGAATATC TAGAGGTTCG ATATTCTGCG
    GACTCACCTC AACTTCCTCA GGATCTAAAA GGAGAAACAT TTTCACATGT GTTTGGAACA
    AATACTTCAA GCCTGGAGCT TTTCTTGCTT AGCAGAAAAA TAAAAGGGCC CAGCTGGCTG
    GAGATAAAAA GCCCACAGCT GAGCAGTCAG CCCATGAGCT GGTGCAAGGT TGAGGCAGTG
    GTGATGAGGC CTGACCAAGT GAATGTTGTG AAGGATCTGG CACCTCCACC TGTTGTTGTT
    TTGTCGCTCA GTATGAAGAC TGTGCAGAAT GCAAAGACTC ACCAAAATGA GATTGTGGCA
    ATGGCTGCTT TGGTTCATCA CAAATTTCCC CTTGATAAAG CACCACCACA GCCACCATTC
    CAGACACATT TTTGTGTTCT GAGCAAACCT AATGATTGCA TTTTCCCATA CGACTTTAAG
    GAAGCGGTGA AACGAAAGAA TGCAAATATT GAGATTGCGT TAACTGAACG AACTCTACTG
    GGCTTTTTCC TAGCAAAAAT TCATAAAATT GATCCAGATA TTATTGTAGG CCATGACATT
    TATGGTTTTG ATCTCGAAGT GTTGCTTCAA AGAATTAGCT CGTGCAAGGT TCCTTTCTGG
    TCCAAAATCG GTAGACTGAG AAGATCTGTT ATGCCAAAAC TTGGGGGTCG AAGTGGCTTT
    GCAGAAAGGA ATGCAGCTTG TGGTCGCATC ATTTGCGATA CTGAGATTTC TGCCAAGGAG
    TTAATTCGAT GTAAAAGCTA TCATTTGTCA GAACTGGTAC ATCAAATATT GAAAACAGAG
    AGAGTTTTGA TGCCACCAGA AAACATCCGC AATGCATACA GTGATTCAGC ACATCTTCTT
    TACATGCTGG AGAACACCTG GACTGATGCA AAATTCATCC TTCAGATCAT GTGTGAGCTG
    AATGTTCTTC CCCTGGCCTT GCAGATCACA AATATTGCAG GCAATGTCTT GTCAAGGACA
    TTAATGGGTG GACGCTCAGA AAGAAACGAG TACCTTCTCC TTCATGCCTT CACAGAAAAT
    AACTTCATAG TGCCAGACAA GCCAGTGTTC AAGAAAATTC AACAGACCAC TGTGGAGGAC
    GATGATAATA TGGACACAGA TCAAAATAAA AATAAGAAAG GTCGAAAGAA GGCTGCCTAT
    GCTGGAGGAT TGGTCCTGGA ACCCAAAGTT GGTTTCTACG ACAAATTCAT CTTGCTTCTG
    GATTTCAACA GCTTGTACCC CTCTATCATT CAGGAATATA ATATCTGCTT TACAACAGTG
    CAAAGAGAGG CTCCTACTTC ACAGAAAGGT GAAGATCAGG AAGAAATTCC AGAGCTACCT
    CATTCTGATC TTGAAATGGG GATCTTACCA CGAGAGATAC GGAAACTGGT GGAGAGGAGG
    CGTCACGTGA AGCAGTTAAT GAAGCAGCCT GACCTAAACC CTGATTTATA CTTACAGTAT
    GACATCAGAC AAAAAGCTTT AAAGCTCACG GCAAACAGCA TGTATGGATG CCTGGGCTTC
    TCCTACAGCA GGTTCTATGC CAAACCTTTG GCTGCATTGG TTACTCACCA AGGGAGAGAG
    ATTTTGCTGC ATACCAAGGA AATGGTACAG AAGATGAATC TGGAAGTCAT ATATGGAGAC
    ACGGATTCTA TCATGATAAA CACCAATTGT AATAATTTAG AAGAAGTCTT CAAGCTTGGA
    AACCGGGTGA AAAGTGAGGT AAACAAATCA TACAAACTTT TAGAGATTGA CATTGATGGT
    ATCTTTAAAT CACTCTTGCT ACTTAAGAAA AAGAAGTATG CCGCTCTGAC TGTGGAGCCT
    ACAGGTGATG GAAAGTATGT TACTAAGCAG GAGCTTAAAG GTTTGGATAT TGTCAGAAGA
    GACTGGTGTG AACTTGCTAA AGAAGCTGGA AATTATGTTA TCAGTCAGAT TCTGTCTGAT
    CAGCCACGTG ACAATATTGT TGAGAACATT CAGAAGAAAT TAACTGAAAT TGGGGAGAAT
    GTAACAAATG GAACTGTGCC CATTAGCCAG TATGAAATAA ACAAGGCATT GACAAAGGAT
    CCTCAGGATT ACCCTGATAA AAAAAGCTTA CCTCACGTAC ATGTTGCCAT GTGGATAAAT
    TCCCAAGGTG GAAGAAAGGT GAAAGCTGGT GATACAATAT CCTATGTCAT TTGCCAGGAC
    GGATCAAATC TTAGTGCCAG CCAGAGAGCA TATGCTCCAG AGCAGCTGCA AAAACAAGAG
    AACCTTTCCA TTGACACACA ATACTACCTG TCACAGCAAG TCCACCCAGT AGTGGCTCGG
    ATCTGTGAGC CCATTGATGG AATTGATTCT GCACTCATTG CAATGTGGTT AGGACTTGAC
    CCCTCCCAGT TCCGGGCTCA CCGCCACCAT CAGCAGGATG AGGAAAATGA TGCATTGTTG
    GGAGGTCCTT CCCAACTCAC AGATGAGGAA AAATACAGAG ATTGTGAACG ATTCAAATTC
    TTCTGCCCAA AGTGTGGAAC AGAGAACATT TATGACAATG TTTTTGATGG CTCTGGGCTG
    CAGATTGAAC CCGGCTTGAA ACGCTGCAGT AAACCAGAGT GTGATGCTTC TCCCTTGGAC
    TACGTTATTC AGGTTCAAAA CAAGCTGCTC TTGGACATAA GACGTTACAT AAAGAAATAC
    TACAGTGGTT GGCTTGTATG TGAAGAAAAA ACCTGCCAGA ACAGAACACG ACGAGTTCCT
    CTGAGCTTCT CCCGCAATGG GCCGATATGC CAAGCTTGCA CTAAAGCAAC GCTAAGATCA
    GAGTATCCGG AAAAGGCCCT GTACACTCAG CTTTGTTTCT ATAGATTCAT TTTTGACTGG
    GATTATGCAC TGGAGAAAGT TGTTTCTGAA CAAGAGAGAG GTCACTTAAA AAAGAAGCAT
    TTTCAAGAGT CAGAAAATCT GTACAAGAAG TTAAAGAGCA CTGTCGACCA AGTACTTTCA
    CAGAGCGGGT ATTCAGAAGT GAACCTCAGC AAGCTGTTCC AAACACTCAG CACCATTAAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52097
    Clone ID Related Accession (Same CDS sequence) XM_031896703.1 , XM_031896704.1
    Accession Version XM_031896703.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4416bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA polymerase alpha catalytic subunit isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCAGAAT CTGGAAGTTT TGCAGCATCA CGTTCGCGGC GTGAAAAAAC AGAAAAGAGC 
    GGCCGTAAAG AAGCATTGGA ACGTTTGAAA AGAGCAAAAG CTGGTGAAAA AGTCAAATAT
    GAGGTGGAAG AAATTTCAAG CATTTATGAG GAGGTGGATG AAGAACAATA CTCTAAAATT
    GTTCGTGACC GACAGGATGA TGATTGGATA GTGGATGATG ATGGGACCGG TTATGTAGAA
    GATGGAAGAG AGATTTTTGA TGATGACTTA GAAGACAATG CTCTTGCTGA TTCAGGTAAA
    AGAGCTAAGG GAGCACCCAA AGATAAATCT AATGTAAAAA AGTCATCGGT GTCGAAGCCA
    AATAATATCA AGTCAATGTT TATGGCCAGT GCTGTCAAAA GGACAACAGA TAAAACTGTG
    GATCTGTCAA AAGATGACTT GCTTGGTGAT TTACTGCAAG ATTTAAAGTC ACAGGCTCTT
    CCTATAACAC CACCCCCAGT AATTACTCTA AAGAAGAAGA AACTTGCAGG GTCTCCTTTA
    AACCCTTTCT CGGTACCACC TGCTGCCCCA AAGGTTTTAC CTGTGAAAAA ACCACCAGCT
    TTAGCAAAGC TTGGGCATCT CTCAAGTGCT GCGCCATCTA ACAACTCTGC CAGTTCTTTT
    GTACCCCGGG AAATTAAGAA TGAGCCAAAT TCTGATTTGA TTTCTTCTGC TCCTTTGAAT
    GTGAAAACTC AAGTAAAAGA AGAGGATACG TCAATGGTTG AGTTCGATGA TGGAGATTTT
    GATGAACCAA TGGAAGAAGA CAATTTGGAA TCTCCTTCAG TTGTTTCTAG CACAATTAAG
    AAGGAAGAAC CAAAAATTAA GTGTGTGAAA GAGGATAGTG TTAAAATGGA GGAAAGTTCT
    TTCATGACTT CCGCTGCTTT AAATGAATCT TGTTGGGATC AAATTGATGA GGTTGAATCA
    ATGACCACTG AAGTTCAGGT AGATTCAAGC CGCCTACCCT TGGTTACAGG TGCAGATGGA
    AGTCAGGTGT TTAAGTTTTA TTGGCTAGAT GCCTATGAAG ACCAGTACAG TCAGCCAGGA
    GTAGTCTACT TATTTGGAAA AGTTTGGATA GAGACAGCTG AGGCGTATGT CAGCTGCTGT
    GTTAGTGTGA AGAACATTGA GAGGACTGTA TATCTCCTAC CTCGTGAAAA TAGAGTGCAG
    CTTTCTACAG GCAAAGACAC TGGAGTACCA GTGTCTATGA TGAATGTATA CCAGGAGTTT
    AATGAAAGTG TTGCAGAAAA GTACAAAATC ATGAAGTTCA AGTCTAAGAA AGTTGAGAAG
    GATTATGCTT TTGAAATTCC TGATGTTCCA GCCAGCTCTG AATATCTAGA GGTTCGATAT
    TCTGCGGACT CACCTCAACT TCCTCAGGAT CTAAAAGGAG AAACATTTTC ACATGTGTTT
    GGAACAAATA CTTCAAGCCT GGAGCTTTTC TTGCTTAGCA GAAAAATAAA AGGGCCCAGC
    TGGCTGGAGA TAAAAAGCCC ACAGCTGAGC AGTCAGCCCA TGAGCTGGTG CAAGGTTGAG
    GCAGTGGTGA TGAGGCCTGA CCAAGTGAAT GTTGTGAAGG ATCTGGCACC TCCACCTGTT
    GTTGTTTTGT CGCTCAGTAT GAAGACTGTG CAGAATGCAA AGACTCACCA AAATGAGATT
    GTGGCAATGG CTGCTTTGGT TCATCACAAA TTTCCCCTTG ATAAAGCACC ACCACAGCCA
    CCATTCCAGA CACATTTTTG TGTTCTGAGC AAACCTAATG ATTGCATTTT CCCATACGAC
    TTTAAGGAAG CGGTGAAACG AAAGAATGCA AATATTGAGA TTGCGTTAAC TGAACGAACT
    CTACTGGGCT TTTTCCTAGC AAAAATTCAT AAAATTGATC CAGATATTAT TGTAGGCCAT
    GACATTTATG GTTTTGATCT CGAAGTGTTG CTTCAAAGAA TTAGCTCGTG CAAGGTTCCT
    TTCTGGTCCA AAATCGGTAG ACTGAGAAGA TCTGTTATGC CAAAACTTGG GGGTCGAAGT
    GGCTTTGCAG AAAGGAATGC AGCTTGTGGT CGCATCATTT GCGATACTGA GATTTCTGCC
    AAGGAGTTAA TTCGATGTAA AAGCTATCAT TTGTCAGAAC TGGTACATCA AATATTGAAA
    ACAGAGAGAG TTTTGATGCC ACCAGAAAAC ATCCGCAATG CATACAGTGA TTCAGCACAT
    CTTCTTTACA TGCTGGAGAA CACCTGGACT GATGCAAAAT TCATCCTTCA GATCATGTGT
    GAGCTGAATG TTCTTCCCCT GGCCTTGCAG ATCACAAATA TTGCAGGCAA TGTCTTGTCA
    AGGACATTAA TGGGTGGACG CTCAGAAAGA AACGAGTACC TTCTCCTTCA TGCCTTCACA
    GAAAATAACT TCATAGTGCC AGACAAGCCA GTGTTCAAGA AAATTCAACA GACCACTGTG
    GAGGACGATG ATAATATGGA CACAGATCAA AATAAAAATA AGAAAGGTCG AAAGAAGGCT
    GCCTATGCTG GAGGATTGGT CCTGGAACCC AAAGTTGGTT TCTACGACAA ATTCATCTTG
    CTTCTGGATT TCAACAGCTT GTACCCCTCT ATCATTCAGG AATATAATAT CTGCTTTACA
    ACAGTGCAAA GAGAGGCTCC TACTTCACAG AAAGGTGAAG ATCAGGAAGA AATTCCAGAG
    CTACCTCATT CTGATCTTGA AATGGGGATC TTACCACGAG AGATACGGAA ACTGGTGGAG
    AGGAGGCGTC ACGTGAAGCA GTTAATGAAG CAGCCTGACC TAAACCCTGA TTTATACTTA
    CAGTATGACA TCAGACAAAA AGCTTTAAAG CTCACGGCAA ACAGCATGTA TGGATGCCTG
    GGCTTCTCCT ACAGCAGGTT CTATGCCAAA CCTTTGGCTG CATTGGTTAC TCACCAAGGG
    AGAGAGATTT TGCTGCATAC CAAGGAAATG GTACAGAAGA TGAATCTGGA AGTCATATAT
    GGAGACACGG ATTCTATCAT GATAAACACC AATTGTAATA ATTTAGAAGA AGTCTTCAAG
    CTTGGAAACC GGGTGAAAAG TGAGGTAAAC AAATCATACA AACTTTTAGA GATTGACATT
    GATGGTATCT TTAAATCACT CTTGCTACTT AAGAAAAAGA AGTATGCCGC TCTGACTGTG
    GAGCCTACAG GTGATGGAAA GTATGTTACT AAGCAGGAGC TTAAAGGTTT GGATATTGTC
    AGAAGAGACT GGTGTGAACT TGCTAAAGAA GCTGGAAATT ATGTTATCAG TCAGATTCTG
    TCTGATCAGC CACGTGACAA TATTGTTGAG AACATTCAGA AGAAATTAAC TGAAATTGGG
    GAGAATGTAA CAAATGGAAC TGTGCCCATT AGCCAGTATG AAATAAACAA GGCATTGACA
    AAGGATCCTC AGGATTACCC TGATAAAAAA AGCTTACCTC ACGTACATGT TGCCATGTGG
    ATAAATTCCC AAGGTGGAAG AAAGGTGAAA GCTGGTGATA CAATATCCTA TGTCATTTGC
    CAGGACGGAT CAAATCTTAG TGCCAGCCAG AGAGCATATG CTCCAGAGCA GCTGCAAAAA
    CAAGAGAACC TTTCCATTGA CACACAATAC TACCTGTCAC AGCAAGTCCA CCCAGTAGTG
    GCTCGGATCT GTGAGCCCAT TGATGGAATT GATTCTGCAC TCATTGCAAT GTGGTTAGGA
    CTTGACCCCT CCCAGTTCCG GGCTCACCGC CACCATCAGC AGGATGAGGA AAATGATGCA
    TTGTTGGGAG GTCCTTCCCA ACTCACAGAT GAGGAAAAAT ACAGAGATTG TGAACGATTC
    AAATTCTTCT GCCCAAAGTG TGGAACAGAG AACATTTATG ACAATGTTTT TGATGGCTCT
    GGGCTGCAGA TTGAACCCGG CTTGAAACGC TGCAGTAAAC CAGAGTGTGA TGCTTCTCCC
    TTGGACTACG TTATTCAGGT TCAAAACAAG CTGCTCTTGG ACATAAGACG TTACATAAAG
    AAATACTACA GTGGTTGGCT TGTATGTGAA GAAAAAACCT GCCAGAACAG AACACGACGA
    GTTCCTCTGA GCTTCTCCCG CAATGGGCCG ATATGCCAAG CTTGCACTAA AGCAACGCTA
    AGATCAGAGT ATCCGGAAAA GGCCCTGTAC ACTCAGCTTT GTTTCTATAG ATTCATTTTT
    GACTGGGATT ATGCACTGGA GAAAGTTGTT TCTGAACAAG AGAGAGTTAT GGAAGATGAT
    CCTGGATGTT TATGCAACTT ACTGGTTGGT CCTGAAATAT CAATCTTTTC TGGCGCTCTT
    ATGATAAGAA TGACTTTGGA AAAAACATTA CTGACTCAAA TAGTGAGCCT TAGACATGCG
    CTGCATTGCT TTTTCTGCAC CTTTACAAAT CTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752563.1
    CDS146..4561
    Translation

    Target ORF information:

    RefSeq Version XM_031896703.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis polymerase (DNA directed), alpha 1, catalytic subunit (pola1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896703.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCAGAAT CTGGAAGTTT TGCAGCATCA CGTTCGCGGC GTGAAAAAAC AGAAAAGAGC 
    GGCCGTAAAG AAGCATTGGA ACGTTTGAAA AGAGCAAAAG CTGGTGAAAA AGTCAAATAT
    GAGGTGGAAG AAATTTCAAG CATTTATGAG GAGGTGGATG AAGAACAATA CTCTAAAATT
    GTTCGTGACC GACAGGATGA TGATTGGATA GTGGATGATG ATGGGACCGG TTATGTAGAA
    GATGGAAGAG AGATTTTTGA TGATGACTTA GAAGACAATG CTCTTGCTGA TTCAGGTAAA
    AGAGCTAAGG GAGCACCCAA AGATAAATCT AATGTAAAAA AGTCATCGGT GTCGAAGCCA
    AATAATATCA AGTCAATGTT TATGGCCAGT GCTGTCAAAA GGACAACAGA TAAAACTGTG
    GATCTGTCAA AAGATGACTT GCTTGGTGAT TTACTGCAAG ATTTAAAGTC ACAGGCTCTT
    CCTATAACAC CACCCCCAGT AATTACTCTA AAGAAGAAGA AACTTGCAGG GTCTCCTTTA
    AACCCTTTCT CGGTACCACC TGCTGCCCCA AAGGTTTTAC CTGTGAAAAA ACCACCAGCT
    TTAGCAAAGC TTGGGCATCT CTCAAGTGCT GCGCCATCTA ACAACTCTGC CAGTTCTTTT
    GTACCCCGGG AAATTAAGAA TGAGCCAAAT TCTGATTTGA TTTCTTCTGC TCCTTTGAAT
    GTGAAAACTC AAGTAAAAGA AGAGGATACG TCAATGGTTG AGTTCGATGA TGGAGATTTT
    GATGAACCAA TGGAAGAAGA CAATTTGGAA TCTCCTTCAG TTGTTTCTAG CACAATTAAG
    AAGGAAGAAC CAAAAATTAA GTGTGTGAAA GAGGATAGTG TTAAAATGGA GGAAAGTTCT
    TTCATGACTT CCGCTGCTTT AAATGAATCT TGTTGGGATC AAATTGATGA GGTTGAATCA
    ATGACCACTG AAGTTCAGGT AGATTCAAGC CGCCTACCCT TGGTTACAGG TGCAGATGGA
    AGTCAGGTGT TTAAGTTTTA TTGGCTAGAT GCCTATGAAG ACCAGTACAG TCAGCCAGGA
    GTAGTCTACT TATTTGGAAA AGTTTGGATA GAGACAGCTG AGGCGTATGT CAGCTGCTGT
    GTTAGTGTGA AGAACATTGA GAGGACTGTA TATCTCCTAC CTCGTGAAAA TAGAGTGCAG
    CTTTCTACAG GCAAAGACAC TGGAGTACCA GTGTCTATGA TGAATGTATA CCAGGAGTTT
    AATGAAAGTG TTGCAGAAAA GTACAAAATC ATGAAGTTCA AGTCTAAGAA AGTTGAGAAG
    GATTATGCTT TTGAAATTCC TGATGTTCCA GCCAGCTCTG AATATCTAGA GGTTCGATAT
    TCTGCGGACT CACCTCAACT TCCTCAGGAT CTAAAAGGAG AAACATTTTC ACATGTGTTT
    GGAACAAATA CTTCAAGCCT GGAGCTTTTC TTGCTTAGCA GAAAAATAAA AGGGCCCAGC
    TGGCTGGAGA TAAAAAGCCC ACAGCTGAGC AGTCAGCCCA TGAGCTGGTG CAAGGTTGAG
    GCAGTGGTGA TGAGGCCTGA CCAAGTGAAT GTTGTGAAGG ATCTGGCACC TCCACCTGTT
    GTTGTTTTGT CGCTCAGTAT GAAGACTGTG CAGAATGCAA AGACTCACCA AAATGAGATT
    GTGGCAATGG CTGCTTTGGT TCATCACAAA TTTCCCCTTG ATAAAGCACC ACCACAGCCA
    CCATTCCAGA CACATTTTTG TGTTCTGAGC AAACCTAATG ATTGCATTTT CCCATACGAC
    TTTAAGGAAG CGGTGAAACG AAAGAATGCA AATATTGAGA TTGCGTTAAC TGAACGAACT
    CTACTGGGCT TTTTCCTAGC AAAAATTCAT AAAATTGATC CAGATATTAT TGTAGGCCAT
    GACATTTATG GTTTTGATCT CGAAGTGTTG CTTCAAAGAA TTAGCTCGTG CAAGGTTCCT
    TTCTGGTCCA AAATCGGTAG ACTGAGAAGA TCTGTTATGC CAAAACTTGG GGGTCGAAGT
    GGCTTTGCAG AAAGGAATGC AGCTTGTGGT CGCATCATTT GCGATACTGA GATTTCTGCC
    AAGGAGTTAA TTCGATGTAA AAGCTATCAT TTGTCAGAAC TGGTACATCA AATATTGAAA
    ACAGAGAGAG TTTTGATGCC ACCAGAAAAC ATCCGCAATG CATACAGTGA TTCAGCACAT
    CTTCTTTACA TGCTGGAGAA CACCTGGACT GATGCAAAAT TCATCCTTCA GATCATGTGT
    GAGCTGAATG TTCTTCCCCT GGCCTTGCAG ATCACAAATA TTGCAGGCAA TGTCTTGTCA
    AGGACATTAA TGGGTGGACG CTCAGAAAGA AACGAGTACC TTCTCCTTCA TGCCTTCACA
    GAAAATAACT TCATAGTGCC AGACAAGCCA GTGTTCAAGA AAATTCAACA GACCACTGTG
    GAGGACGATG ATAATATGGA CACAGATCAA AATAAAAATA AGAAAGGTCG AAAGAAGGCT
    GCCTATGCTG GAGGATTGGT CCTGGAACCC AAAGTTGGTT TCTACGACAA ATTCATCTTG
    CTTCTGGATT TCAACAGCTT GTACCCCTCT ATCATTCAGG AATATAATAT CTGCTTTACA
    ACAGTGCAAA GAGAGGCTCC TACTTCACAG AAAGGTGAAG ATCAGGAAGA AATTCCAGAG
    CTACCTCATT CTGATCTTGA AATGGGGATC TTACCACGAG AGATACGGAA ACTGGTGGAG
    AGGAGGCGTC ACGTGAAGCA GTTAATGAAG CAGCCTGACC TAAACCCTGA TTTATACTTA
    CAGTATGACA TCAGACAAAA AGCTTTAAAG CTCACGGCAA ACAGCATGTA TGGATGCCTG
    GGCTTCTCCT ACAGCAGGTT CTATGCCAAA CCTTTGGCTG CATTGGTTAC TCACCAAGGG
    AGAGAGATTT TGCTGCATAC CAAGGAAATG GTACAGAAGA TGAATCTGGA AGTCATATAT
    GGAGACACGG ATTCTATCAT GATAAACACC AATTGTAATA ATTTAGAAGA AGTCTTCAAG
    CTTGGAAACC GGGTGAAAAG TGAGGTAAAC AAATCATACA AACTTTTAGA GATTGACATT
    GATGGTATCT TTAAATCACT CTTGCTACTT AAGAAAAAGA AGTATGCCGC TCTGACTGTG
    GAGCCTACAG GTGATGGAAA GTATGTTACT AAGCAGGAGC TTAAAGGTTT GGATATTGTC
    AGAAGAGACT GGTGTGAACT TGCTAAAGAA GCTGGAAATT ATGTTATCAG TCAGATTCTG
    TCTGATCAGC CACGTGACAA TATTGTTGAG AACATTCAGA AGAAATTAAC TGAAATTGGG
    GAGAATGTAA CAAATGGAAC TGTGCCCATT AGCCAGTATG AAATAAACAA GGCATTGACA
    AAGGATCCTC AGGATTACCC TGATAAAAAA AGCTTACCTC ACGTACATGT TGCCATGTGG
    ATAAATTCCC AAGGTGGAAG AAAGGTGAAA GCTGGTGATA CAATATCCTA TGTCATTTGC
    CAGGACGGAT CAAATCTTAG TGCCAGCCAG AGAGCATATG CTCCAGAGCA GCTGCAAAAA
    CAAGAGAACC TTTCCATTGA CACACAATAC TACCTGTCAC AGCAAGTCCA CCCAGTAGTG
    GCTCGGATCT GTGAGCCCAT TGATGGAATT GATTCTGCAC TCATTGCAAT GTGGTTAGGA
    CTTGACCCCT CCCAGTTCCG GGCTCACCGC CACCATCAGC AGGATGAGGA AAATGATGCA
    TTGTTGGGAG GTCCTTCCCA ACTCACAGAT GAGGAAAAAT ACAGAGATTG TGAACGATTC
    AAATTCTTCT GCCCAAAGTG TGGAACAGAG AACATTTATG ACAATGTTTT TGATGGCTCT
    GGGCTGCAGA TTGAACCCGG CTTGAAACGC TGCAGTAAAC CAGAGTGTGA TGCTTCTCCC
    TTGGACTACG TTATTCAGGT TCAAAACAAG CTGCTCTTGG ACATAAGACG TTACATAAAG
    AAATACTACA GTGGTTGGCT TGTATGTGAA GAAAAAACCT GCCAGAACAG AACACGACGA
    GTTCCTCTGA GCTTCTCCCG CAATGGGCCG ATATGCCAAG CTTGCACTAA AGCAACGCTA
    AGATCAGAGT ATCCGGAAAA GGCCCTGTAC ACTCAGCTTT GTTTCTATAG ATTCATTTTT
    GACTGGGATT ATGCACTGGA GAAAGTTGTT TCTGAACAAG AGAGAGTTAT GGAAGATGAT
    CCTGGATGTT TATGCAACTT ACTGGTTGGT CCTGAAATAT CAATCTTTTC TGGCGCTCTT
    ATGATAAGAA TGACTTTGGA AAAAACATTA CTGACTCAAA TAGTGAGCCT TAGACATGCG
    CTGCATTGCT TTTTCTGCAC CTTTACAAAT CTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52097
    Clone ID Related Accession (Same CDS sequence) XM_031896703.1 , XM_031896704.1
    Accession Version XM_031896704.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4416bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA polymerase alpha catalytic subunit isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCAGAAT CTGGAAGTTT TGCAGCATCA CGTTCGCGGC GTGAAAAAAC AGAAAAGAGC 
    GGCCGTAAAG AAGCATTGGA ACGTTTGAAA AGAGCAAAAG CTGGTGAAAA AGTCAAATAT
    GAGGTGGAAG AAATTTCAAG CATTTATGAG GAGGTGGATG AAGAACAATA CTCTAAAATT
    GTTCGTGACC GACAGGATGA TGATTGGATA GTGGATGATG ATGGGACCGG TTATGTAGAA
    GATGGAAGAG AGATTTTTGA TGATGACTTA GAAGACAATG CTCTTGCTGA TTCAGGTAAA
    AGAGCTAAGG GAGCACCCAA AGATAAATCT AATGTAAAAA AGTCATCGGT GTCGAAGCCA
    AATAATATCA AGTCAATGTT TATGGCCAGT GCTGTCAAAA GGACAACAGA TAAAACTGTG
    GATCTGTCAA AAGATGACTT GCTTGGTGAT TTACTGCAAG ATTTAAAGTC ACAGGCTCTT
    CCTATAACAC CACCCCCAGT AATTACTCTA AAGAAGAAGA AACTTGCAGG GTCTCCTTTA
    AACCCTTTCT CGGTACCACC TGCTGCCCCA AAGGTTTTAC CTGTGAAAAA ACCACCAGCT
    TTAGCAAAGC TTGGGCATCT CTCAAGTGCT GCGCCATCTA ACAACTCTGC CAGTTCTTTT
    GTACCCCGGG AAATTAAGAA TGAGCCAAAT TCTGATTTGA TTTCTTCTGC TCCTTTGAAT
    GTGAAAACTC AAGTAAAAGA AGAGGATACG TCAATGGTTG AGTTCGATGA TGGAGATTTT
    GATGAACCAA TGGAAGAAGA CAATTTGGAA TCTCCTTCAG TTGTTTCTAG CACAATTAAG
    AAGGAAGAAC CAAAAATTAA GTGTGTGAAA GAGGATAGTG TTAAAATGGA GGAAAGTTCT
    TTCATGACTT CCGCTGCTTT AAATGAATCT TGTTGGGATC AAATTGATGA GGTTGAATCA
    ATGACCACTG AAGTTCAGGT AGATTCAAGC CGCCTACCCT TGGTTACAGG TGCAGATGGA
    AGTCAGGTGT TTAAGTTTTA TTGGCTAGAT GCCTATGAAG ACCAGTACAG TCAGCCAGGA
    GTAGTCTACT TATTTGGAAA AGTTTGGATA GAGACAGCTG AGGCGTATGT CAGCTGCTGT
    GTTAGTGTGA AGAACATTGA GAGGACTGTA TATCTCCTAC CTCGTGAAAA TAGAGTGCAG
    CTTTCTACAG GCAAAGACAC TGGAGTACCA GTGTCTATGA TGAATGTATA CCAGGAGTTT
    AATGAAAGTG TTGCAGAAAA GTACAAAATC ATGAAGTTCA AGTCTAAGAA AGTTGAGAAG
    GATTATGCTT TTGAAATTCC TGATGTTCCA GCCAGCTCTG AATATCTAGA GGTTCGATAT
    TCTGCGGACT CACCTCAACT TCCTCAGGAT CTAAAAGGAG AAACATTTTC ACATGTGTTT
    GGAACAAATA CTTCAAGCCT GGAGCTTTTC TTGCTTAGCA GAAAAATAAA AGGGCCCAGC
    TGGCTGGAGA TAAAAAGCCC ACAGCTGAGC AGTCAGCCCA TGAGCTGGTG CAAGGTTGAG
    GCAGTGGTGA TGAGGCCTGA CCAAGTGAAT GTTGTGAAGG ATCTGGCACC TCCACCTGTT
    GTTGTTTTGT CGCTCAGTAT GAAGACTGTG CAGAATGCAA AGACTCACCA AAATGAGATT
    GTGGCAATGG CTGCTTTGGT TCATCACAAA TTTCCCCTTG ATAAAGCACC ACCACAGCCA
    CCATTCCAGA CACATTTTTG TGTTCTGAGC AAACCTAATG ATTGCATTTT CCCATACGAC
    TTTAAGGAAG CGGTGAAACG AAAGAATGCA AATATTGAGA TTGCGTTAAC TGAACGAACT
    CTACTGGGCT TTTTCCTAGC AAAAATTCAT AAAATTGATC CAGATATTAT TGTAGGCCAT
    GACATTTATG GTTTTGATCT CGAAGTGTTG CTTCAAAGAA TTAGCTCGTG CAAGGTTCCT
    TTCTGGTCCA AAATCGGTAG ACTGAGAAGA TCTGTTATGC CAAAACTTGG GGGTCGAAGT
    GGCTTTGCAG AAAGGAATGC AGCTTGTGGT CGCATCATTT GCGATACTGA GATTTCTGCC
    AAGGAGTTAA TTCGATGTAA AAGCTATCAT TTGTCAGAAC TGGTACATCA AATATTGAAA
    ACAGAGAGAG TTTTGATGCC ACCAGAAAAC ATCCGCAATG CATACAGTGA TTCAGCACAT
    CTTCTTTACA TGCTGGAGAA CACCTGGACT GATGCAAAAT TCATCCTTCA GATCATGTGT
    GAGCTGAATG TTCTTCCCCT GGCCTTGCAG ATCACAAATA TTGCAGGCAA TGTCTTGTCA
    AGGACATTAA TGGGTGGACG CTCAGAAAGA AACGAGTACC TTCTCCTTCA TGCCTTCACA
    GAAAATAACT TCATAGTGCC AGACAAGCCA GTGTTCAAGA AAATTCAACA GACCACTGTG
    GAGGACGATG ATAATATGGA CACAGATCAA AATAAAAATA AGAAAGGTCG AAAGAAGGCT
    GCCTATGCTG GAGGATTGGT CCTGGAACCC AAAGTTGGTT TCTACGACAA ATTCATCTTG
    CTTCTGGATT TCAACAGCTT GTACCCCTCT ATCATTCAGG AATATAATAT CTGCTTTACA
    ACAGTGCAAA GAGAGGCTCC TACTTCACAG AAAGGTGAAG ATCAGGAAGA AATTCCAGAG
    CTACCTCATT CTGATCTTGA AATGGGGATC TTACCACGAG AGATACGGAA ACTGGTGGAG
    AGGAGGCGTC ACGTGAAGCA GTTAATGAAG CAGCCTGACC TAAACCCTGA TTTATACTTA
    CAGTATGACA TCAGACAAAA AGCTTTAAAG CTCACGGCAA ACAGCATGTA TGGATGCCTG
    GGCTTCTCCT ACAGCAGGTT CTATGCCAAA CCTTTGGCTG CATTGGTTAC TCACCAAGGG
    AGAGAGATTT TGCTGCATAC CAAGGAAATG GTACAGAAGA TGAATCTGGA AGTCATATAT
    GGAGACACGG ATTCTATCAT GATAAACACC AATTGTAATA ATTTAGAAGA AGTCTTCAAG
    CTTGGAAACC GGGTGAAAAG TGAGGTAAAC AAATCATACA AACTTTTAGA GATTGACATT
    GATGGTATCT TTAAATCACT CTTGCTACTT AAGAAAAAGA AGTATGCCGC TCTGACTGTG
    GAGCCTACAG GTGATGGAAA GTATGTTACT AAGCAGGAGC TTAAAGGTTT GGATATTGTC
    AGAAGAGACT GGTGTGAACT TGCTAAAGAA GCTGGAAATT ATGTTATCAG TCAGATTCTG
    TCTGATCAGC CACGTGACAA TATTGTTGAG AACATTCAGA AGAAATTAAC TGAAATTGGG
    GAGAATGTAA CAAATGGAAC TGTGCCCATT AGCCAGTATG AAATAAACAA GGCATTGACA
    AAGGATCCTC AGGATTACCC TGATAAAAAA AGCTTACCTC ACGTACATGT TGCCATGTGG
    ATAAATTCCC AAGGTGGAAG AAAGGTGAAA GCTGGTGATA CAATATCCTA TGTCATTTGC
    CAGGACGGAT CAAATCTTAG TGCCAGCCAG AGAGCATATG CTCCAGAGCA GCTGCAAAAA
    CAAGAGAACC TTTCCATTGA CACACAATAC TACCTGTCAC AGCAAGTCCA CCCAGTAGTG
    GCTCGGATCT GTGAGCCCAT TGATGGAATT GATTCTGCAC TCATTGCAAT GTGGTTAGGA
    CTTGACCCCT CCCAGTTCCG GGCTCACCGC CACCATCAGC AGGATGAGGA AAATGATGCA
    TTGTTGGGAG GTCCTTCCCA ACTCACAGAT GAGGAAAAAT ACAGAGATTG TGAACGATTC
    AAATTCTTCT GCCCAAAGTG TGGAACAGAG AACATTTATG ACAATGTTTT TGATGGCTCT
    GGGCTGCAGA TTGAACCCGG CTTGAAACGC TGCAGTAAAC CAGAGTGTGA TGCTTCTCCC
    TTGGACTACG TTATTCAGGT TCAAAACAAG CTGCTCTTGG ACATAAGACG TTACATAAAG
    AAATACTACA GTGGTTGGCT TGTATGTGAA GAAAAAACCT GCCAGAACAG AACACGACGA
    GTTCCTCTGA GCTTCTCCCG CAATGGGCCG ATATGCCAAG CTTGCACTAA AGCAACGCTA
    AGATCAGAGT ATCCGGAAAA GGCCCTGTAC ACTCAGCTTT GTTTCTATAG ATTCATTTTT
    GACTGGGATT ATGCACTGGA GAAAGTTGTT TCTGAACAAG AGAGAGTTAT GGAAGATGAT
    CCTGGATGTT TATGCAACTT ACTGGTTGGT CCTGAAATAT CAATCTTTTC TGGCGCTCTT
    ATGATAAGAA TGACTTTGGA AAAAACATTA CTGACTCAAA TAGTGAGCCT TAGACATGCG
    CTGCATTGCT TTTTCTGCAC CTTTACAAAT CTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752564.1
    CDS142..4557
    Translation

    Target ORF information:

    RefSeq Version XM_031896704.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis polymerase (DNA directed), alpha 1, catalytic subunit (pola1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896704.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCAGAAT CTGGAAGTTT TGCAGCATCA CGTTCGCGGC GTGAAAAAAC AGAAAAGAGC 
    GGCCGTAAAG AAGCATTGGA ACGTTTGAAA AGAGCAAAAG CTGGTGAAAA AGTCAAATAT
    GAGGTGGAAG AAATTTCAAG CATTTATGAG GAGGTGGATG AAGAACAATA CTCTAAAATT
    GTTCGTGACC GACAGGATGA TGATTGGATA GTGGATGATG ATGGGACCGG TTATGTAGAA
    GATGGAAGAG AGATTTTTGA TGATGACTTA GAAGACAATG CTCTTGCTGA TTCAGGTAAA
    AGAGCTAAGG GAGCACCCAA AGATAAATCT AATGTAAAAA AGTCATCGGT GTCGAAGCCA
    AATAATATCA AGTCAATGTT TATGGCCAGT GCTGTCAAAA GGACAACAGA TAAAACTGTG
    GATCTGTCAA AAGATGACTT GCTTGGTGAT TTACTGCAAG ATTTAAAGTC ACAGGCTCTT
    CCTATAACAC CACCCCCAGT AATTACTCTA AAGAAGAAGA AACTTGCAGG GTCTCCTTTA
    AACCCTTTCT CGGTACCACC TGCTGCCCCA AAGGTTTTAC CTGTGAAAAA ACCACCAGCT
    TTAGCAAAGC TTGGGCATCT CTCAAGTGCT GCGCCATCTA ACAACTCTGC CAGTTCTTTT
    GTACCCCGGG AAATTAAGAA TGAGCCAAAT TCTGATTTGA TTTCTTCTGC TCCTTTGAAT
    GTGAAAACTC AAGTAAAAGA AGAGGATACG TCAATGGTTG AGTTCGATGA TGGAGATTTT
    GATGAACCAA TGGAAGAAGA CAATTTGGAA TCTCCTTCAG TTGTTTCTAG CACAATTAAG
    AAGGAAGAAC CAAAAATTAA GTGTGTGAAA GAGGATAGTG TTAAAATGGA GGAAAGTTCT
    TTCATGACTT CCGCTGCTTT AAATGAATCT TGTTGGGATC AAATTGATGA GGTTGAATCA
    ATGACCACTG AAGTTCAGGT AGATTCAAGC CGCCTACCCT TGGTTACAGG TGCAGATGGA
    AGTCAGGTGT TTAAGTTTTA TTGGCTAGAT GCCTATGAAG ACCAGTACAG TCAGCCAGGA
    GTAGTCTACT TATTTGGAAA AGTTTGGATA GAGACAGCTG AGGCGTATGT CAGCTGCTGT
    GTTAGTGTGA AGAACATTGA GAGGACTGTA TATCTCCTAC CTCGTGAAAA TAGAGTGCAG
    CTTTCTACAG GCAAAGACAC TGGAGTACCA GTGTCTATGA TGAATGTATA CCAGGAGTTT
    AATGAAAGTG TTGCAGAAAA GTACAAAATC ATGAAGTTCA AGTCTAAGAA AGTTGAGAAG
    GATTATGCTT TTGAAATTCC TGATGTTCCA GCCAGCTCTG AATATCTAGA GGTTCGATAT
    TCTGCGGACT CACCTCAACT TCCTCAGGAT CTAAAAGGAG AAACATTTTC ACATGTGTTT
    GGAACAAATA CTTCAAGCCT GGAGCTTTTC TTGCTTAGCA GAAAAATAAA AGGGCCCAGC
    TGGCTGGAGA TAAAAAGCCC ACAGCTGAGC AGTCAGCCCA TGAGCTGGTG CAAGGTTGAG
    GCAGTGGTGA TGAGGCCTGA CCAAGTGAAT GTTGTGAAGG ATCTGGCACC TCCACCTGTT
    GTTGTTTTGT CGCTCAGTAT GAAGACTGTG CAGAATGCAA AGACTCACCA AAATGAGATT
    GTGGCAATGG CTGCTTTGGT TCATCACAAA TTTCCCCTTG ATAAAGCACC ACCACAGCCA
    CCATTCCAGA CACATTTTTG TGTTCTGAGC AAACCTAATG ATTGCATTTT CCCATACGAC
    TTTAAGGAAG CGGTGAAACG AAAGAATGCA AATATTGAGA TTGCGTTAAC TGAACGAACT
    CTACTGGGCT TTTTCCTAGC AAAAATTCAT AAAATTGATC CAGATATTAT TGTAGGCCAT
    GACATTTATG GTTTTGATCT CGAAGTGTTG CTTCAAAGAA TTAGCTCGTG CAAGGTTCCT
    TTCTGGTCCA AAATCGGTAG ACTGAGAAGA TCTGTTATGC CAAAACTTGG GGGTCGAAGT
    GGCTTTGCAG AAAGGAATGC AGCTTGTGGT CGCATCATTT GCGATACTGA GATTTCTGCC
    AAGGAGTTAA TTCGATGTAA AAGCTATCAT TTGTCAGAAC TGGTACATCA AATATTGAAA
    ACAGAGAGAG TTTTGATGCC ACCAGAAAAC ATCCGCAATG CATACAGTGA TTCAGCACAT
    CTTCTTTACA TGCTGGAGAA CACCTGGACT GATGCAAAAT TCATCCTTCA GATCATGTGT
    GAGCTGAATG TTCTTCCCCT GGCCTTGCAG ATCACAAATA TTGCAGGCAA TGTCTTGTCA
    AGGACATTAA TGGGTGGACG CTCAGAAAGA AACGAGTACC TTCTCCTTCA TGCCTTCACA
    GAAAATAACT TCATAGTGCC AGACAAGCCA GTGTTCAAGA AAATTCAACA GACCACTGTG
    GAGGACGATG ATAATATGGA CACAGATCAA AATAAAAATA AGAAAGGTCG AAAGAAGGCT
    GCCTATGCTG GAGGATTGGT CCTGGAACCC AAAGTTGGTT TCTACGACAA ATTCATCTTG
    CTTCTGGATT TCAACAGCTT GTACCCCTCT ATCATTCAGG AATATAATAT CTGCTTTACA
    ACAGTGCAAA GAGAGGCTCC TACTTCACAG AAAGGTGAAG ATCAGGAAGA AATTCCAGAG
    CTACCTCATT CTGATCTTGA AATGGGGATC TTACCACGAG AGATACGGAA ACTGGTGGAG
    AGGAGGCGTC ACGTGAAGCA GTTAATGAAG CAGCCTGACC TAAACCCTGA TTTATACTTA
    CAGTATGACA TCAGACAAAA AGCTTTAAAG CTCACGGCAA ACAGCATGTA TGGATGCCTG
    GGCTTCTCCT ACAGCAGGTT CTATGCCAAA CCTTTGGCTG CATTGGTTAC TCACCAAGGG
    AGAGAGATTT TGCTGCATAC CAAGGAAATG GTACAGAAGA TGAATCTGGA AGTCATATAT
    GGAGACACGG ATTCTATCAT GATAAACACC AATTGTAATA ATTTAGAAGA AGTCTTCAAG
    CTTGGAAACC GGGTGAAAAG TGAGGTAAAC AAATCATACA AACTTTTAGA GATTGACATT
    GATGGTATCT TTAAATCACT CTTGCTACTT AAGAAAAAGA AGTATGCCGC TCTGACTGTG
    GAGCCTACAG GTGATGGAAA GTATGTTACT AAGCAGGAGC TTAAAGGTTT GGATATTGTC
    AGAAGAGACT GGTGTGAACT TGCTAAAGAA GCTGGAAATT ATGTTATCAG TCAGATTCTG
    TCTGATCAGC CACGTGACAA TATTGTTGAG AACATTCAGA AGAAATTAAC TGAAATTGGG
    GAGAATGTAA CAAATGGAAC TGTGCCCATT AGCCAGTATG AAATAAACAA GGCATTGACA
    AAGGATCCTC AGGATTACCC TGATAAAAAA AGCTTACCTC ACGTACATGT TGCCATGTGG
    ATAAATTCCC AAGGTGGAAG AAAGGTGAAA GCTGGTGATA CAATATCCTA TGTCATTTGC
    CAGGACGGAT CAAATCTTAG TGCCAGCCAG AGAGCATATG CTCCAGAGCA GCTGCAAAAA
    CAAGAGAACC TTTCCATTGA CACACAATAC TACCTGTCAC AGCAAGTCCA CCCAGTAGTG
    GCTCGGATCT GTGAGCCCAT TGATGGAATT GATTCTGCAC TCATTGCAAT GTGGTTAGGA
    CTTGACCCCT CCCAGTTCCG GGCTCACCGC CACCATCAGC AGGATGAGGA AAATGATGCA
    TTGTTGGGAG GTCCTTCCCA ACTCACAGAT GAGGAAAAAT ACAGAGATTG TGAACGATTC
    AAATTCTTCT GCCCAAAGTG TGGAACAGAG AACATTTATG ACAATGTTTT TGATGGCTCT
    GGGCTGCAGA TTGAACCCGG CTTGAAACGC TGCAGTAAAC CAGAGTGTGA TGCTTCTCCC
    TTGGACTACG TTATTCAGGT TCAAAACAAG CTGCTCTTGG ACATAAGACG TTACATAAAG
    AAATACTACA GTGGTTGGCT TGTATGTGAA GAAAAAACCT GCCAGAACAG AACACGACGA
    GTTCCTCTGA GCTTCTCCCG CAATGGGCCG ATATGCCAAG CTTGCACTAA AGCAACGCTA
    AGATCAGAGT ATCCGGAAAA GGCCCTGTAC ACTCAGCTTT GTTTCTATAG ATTCATTTTT
    GACTGGGATT ATGCACTGGA GAAAGTTGTT TCTGAACAAG AGAGAGTTAT GGAAGATGAT
    CCTGGATGTT TATGCAACTT ACTGGTTGGT CCTGAAATAT CAATCTTTTC TGGCGCTCTT
    ATGATAAGAA TGACTTTGGA AAAAACATTA CTGACTCAAA TAGTGAGCCT TAGACATGCG
    CTGCATTGCT TTTTCTGCAC CTTTACAAAT CTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa05773
    Clone ID Related Accession (Same CDS sequence) XM_002933612.4 , XM_004911754.3
    Accession Version XM_002933612.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4389bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA polymerase alpha catalytic subunit isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002933612.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCAGAAT CTGGAAGTTT TGCAGCATCA CGTTCGCGGC GTGAAAAAAC AGAAAAGAGC 
    GGCCGTAAAG AAGCATTGGA ACGTTTGAAA AGAGCAAAAG CTGGTGAAAA AGTCAAATAT
    GAGGTGGAAG AAATTTCAAG CATTTATGAG GAGGTGGATG AAGAACAATA CTCTAAAATT
    GTTCGTGACC GACAGGATGA TGATTGGATA GTGGATGATG ATGGGACCGG TTATGTAGAA
    GATGGAAGAG AGATTTTTGA TGATGACTTA GAAGACAATG CTCTTGCTGA TTCAGGTAAA
    AGAGCTAAGG GAGCACCCAA AGATAAATCT AATGTAAAAA AGTCATCGGT GTCGAAGCCA
    AATAATATCA AGTCAATGTT TATGGCCAGT GCTGTCAAAA GGACAACAGA TAAAACTGTG
    GATCTGTCAA AAGATGACTT GCTTGGTGAT TTACTGCAAG ATTTAAAGTC ACAGGCTCTT
    CCTATAACAC CACCCCCAGT AATTACTCTA AAGAAGAAGA AACTTGCAGG GTCTCCTTTA
    AACCCTTTCT CGGTACCACC TGCTGCCCCA AAGGTTTTAC CTGTGAAAAA ACCACCAGCT
    TTAGCAAAGC TTGGGCATCT CTCAAGTGCT GCGCCATCTA ACAACTCTGC CAGTTCTTTT
    GTACCCCGGG AAATTAAGAA TGAGCCAAAT TCTGATTTGA TTTCTTCTGC TCCTTTGAAT
    GTGAAAACTC AAGTAAAAGA AGAGGATACG TCAATGGTTG AGTTCGATGA TGGAGATTTT
    GATGAACCAA TGGAAGAAGA CAATTTGGAA TCTCCTTCAG TTGTTTCTAG CACAATTAAG
    AAGGAAGAAC CAAAAATTAA GTGTGTGAAA GAGGATAGTG TTAAAATGGA GGAAAGTTCT
    TTCATGACTT CCGCTGCTTT AAATGAATCT TGTTGGGATC AAATTGATGA GGTTGAATCA
    ATGACCACTG AAGTTCAGGT AGATTCAAGC CGCCTACCCT TGGTTACAGG TGCAGATGGA
    AGTCAGGTGT TTAAGTTTTA TTGGCTAGAT GCCTATGAAG ACCAGTACAG TCAGCCAGGA
    GTAGTCTACT TATTTGGAAA AGTTTGGATA GAGACAGCTG AGGCGTATGT CAGCTGCTGT
    GTTAGTGTGA AGAACATTGA GAGGACTGTA TATCTCCTAC CTCGTGAAAA TAGAGTGCAG
    CTTTCTACAG GCAAAGACAC TGGAGTACCA GTGTCTATGA TGAATGTATA CCAGGAGTTT
    AATGAAAGTG TTGCAGAAAA GTACAAAATC ATGAAGTTCA AGTCTAAGAA AGTTGAGAAG
    GATTATGCTT TTGAAATTCC TGATGTTCCA GCCAGCTCTG AATATCTAGA GGTTCGATAT
    TCTGCGGACT CACCTCAACT TCCTCAGGAT CTAAAAGGAG AAACATTTTC ACATGTGTTT
    GGAACAAATA CTTCAAGCCT GGAGCTTTTC TTGCTTAGCA GAAAAATAAA AGGGCCCAGC
    TGGCTGGAGA TAAAAAGCCC ACAGCTGAGC AGTCAGCCCA TGAGCTGGTG CAAGGTTGAG
    GCAGTGGTGA TGAGGCCTGA CCAAGTGAAT GTTGTGAAGG ATCTGGCACC TCCACCTGTT
    GTTGTTTTGT CGCTCAGTAT GAAGACTGTG CAGAATGCAA AGACTCACCA AAATGAGATT
    GTGGCAATGG CTGCTTTGGT TCATCACAAA TTTCCCTTTG ATAAAGCACC ACCACAGCCA
    CCATTCCAGA CACATTTTTG TGTTCTGAGC AAACCTAATG ATTGCATTTT CCCATACGAC
    TTTAAGGAAG CGGTGAAACG AAAGAATGCA AATATTGAGA TTGCGTTAAC TGAACGAACT
    CTACTGGGCT TTTTCCTAGC AAAAATTCAT AAAATTGATC CAGATATTAT TGTAGGCCAT
    GACATTTATG GTTTTGATCT CGAAGTGTTG CTTCAAAGAA TTAGCTCGTG CAAGGTTCCT
    TTCTGGTCCA AAATCGGTAG ACTGAGAAGA TCTGTTATGC CAAAACTTGG GGGTCGAAGT
    GGCTTTGCAG AAAGGAATGC AGCTTGTGGT CGCATCATTT GCGATACTGA GATTTCTGCC
    AAGGAGTTAA TTCGATGTAA AAGCTATCAT TTGTCAGAAC TGGTACATCA AATATTGAAA
    ACAGAGAGAG TTTTGATGCC ACCAGAAAAC ATCCGCAATG CATACAGTGA TTCAGCACAT
    CTTCTTTACA TGCTGGAGAA CACCTGGACT GATGCAAAAT TCATCCTTCA GATCATGTGT
    GAGCTGAATG TTCTTCCCCT GGCCTTGCAG ATCACAAATA TTGCAGGCAA TGTCTTGTCA
    AGGACATTAA TGGGTGGACG CTCAGAAAGA AACGAGTACC TTCTCCTTCA TGCCTTCACA
    GAAAATAACT TCATAGTGCC AGACAAGCCA GTGTTCAAGA AAATTCAACA GACCACTGTG
    GAGGACGATG ATAATATGGA CACAGATCAA AATAAAAATA AGAAAGGTCG AAAGAAGGCT
    GCCTATGCTG GAGGATTGGT CCTGGAACCC AAAGTTGGTT TCTACGACAA ATTCATCTTG
    CTTCTGGATT TCAACAGCTT GTACCCCTCT ATCATTCAGG AATATAATAT CTGCTTTACA
    ACAGTGCAAA GAGAGGCTCC TACTTCACAG AAAGGTGAAG ATCAGGAAGA AATTCCAGAG
    CTACCTCATT CTGATCTTGA AATGGGGATC TTACCACGAG AGATACGGAA ACTGGTGGAG
    AGGAGGCGTC ACGTGAAGCA GTTAATGAAG CAGCCTGACC TAAACCCTGA TTTATACTTA
    CAGTATGACA TCAGACAAAA AGCTTTAAAG CTCACGGCAA ACAGCATGTA TGGATGCCTG
    GGCTTCTCCT ACAGCAGGTT CTATGCCAAA CCTTTGGCTG CATTGGTTAC TCACCAAGGG
    AGAGAGATTT TGCTGCATAC CAAGGAAATG GTACAGAAGA TGAATCTGGA AGTCATATAT
    GGAGACACGG ATTCTATCAT GATAAACACC AATTGTAATA ATTTAGAAGA AGTCTTCAAG
    CTTGGAAACC GGGTGAAAAG TGAGGTAAAC AAATCATACA AACTTTTAGA GATTGACATT
    GATGGTATCT TTAAATCACT CTTGCTACTT AAGAAAAAGA AGTATGCCGC TCTGACTGTG
    GAGCCTACAG GTGATGGAAA GTATGTTACT AAGCAGGAGC TTAAAGGTTT GGATATTGTC
    AGAAGAGACT GGTGTGAACT TGCTAAAGAA GCTGGAAATT ATGTTATCAG TCAGATTCTG
    TCTGATCAGC CACGTGACAA TATTGTTGAG AACATTCAGA AGAAATTAAC TGAAATTGGG
    GAGAATGTAA CAAATGGAAC TGTGCCCATT AGCCAGTATG AAATAAACAA GGCATTGACA
    AAGGATCCTC AGGATTACCC TGATAAAAAA AGCTTACCTC ACGTACATGT TGCCATGTGG
    ATAAATTCCC AAGGTGGAAG AAAGGTGAAA GCTGGTGATA CAATATCCTA TGTCATTTGC
    CAGGACGGAT CAAATCTTAG TGCCAGCCAG AGAGCATATG CTCCAGAGCA GCTGCAAAAA
    CAAGAGAACC TTTCCATTGA CACACAATAC TACCTGTCAC AGCAAGTCCA CCCAGTAGTG
    GCTCGGATCT GTGAGCCCAT TGATGGAATT GATTCTGCAC TCATTGCAAT GTGGTTAGGA
    CTTGACCCCT CCCAGTTCCG GGCTCACCGC CACCATCAGC AGGATGAGGA AAATGATGCA
    TTGTTGGGAG GTCCTTCCCA ACTCACAGAT GAGGAAAAAT ACAGAGATTG TGAACGATTC
    AAATTCTTCT GCCCAAAGTG TGGAACAGAG AACATTTATG ACAATGTTTT TGATGGCTCT
    GGGCTGCAGA TTGAACCCGG CTTGAAACGC TGCAGTAAAC CAGAGTGTGA TGCTTCTCCC
    TTGGACTACG TTATTCAGGT TCAAAACAAG CTGCTCTTGG ACATAAGACG TTACATAAAG
    AAATACTACA GTGGTTGGCT TGTATGTGAA GAAAAAACCT GCCAGAACAG AACACGACGA
    GTTCCTCTGA GCTTCTCCCG CAATGGGCCG ATATGCCAAG CTTGCACTAA AGCAACGCTA
    AGATCAGAGT ATCCGGAAAA GGCCCTGTAC ACTCAGCTTT GTTTCTATAG ATTCATTTTT
    GACTGGGATT ATGCACTGGA GAAAGTTGTT TCTGAACAAG AGAGAGGTCA CTTAAAAAAG
    AAGCATTTTC AAGAGTCAGA AAATCTGTAC AAGAAGTTAA AGAGCACTGT CGACCAAGTA
    CTTTCACAGA GCGGGTATTC AGAAGTGAAC CTCAGCAAGC TGTTCCAAAC ACTCAGCACC
    ATTAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002933658.1
    CDS170..4558
    Translation

    Target ORF information:

    RefSeq Version XM_002933612.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis polymerase (DNA directed), alpha 1, catalytic subunit (pola1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002933612.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCAGAAT CTGGAAGTTT TGCAGCATCA CGTTCGCGGC GTGAAAAAAC AGAAAAGAGC 
    GGCCGTAAAG AAGCATTGGA ACGTTTGAAA AGAGCAAAAG CTGGTGAAAA AGTCAAATAT
    GAGGTGGAAG AAATTTCAAG CATTTATGAG GAGGTGGATG AAGAACAATA CTCTAAAATT
    GTTCGTGACC GACAGGATGA TGATTGGATA GTGGATGATG ATGGGACCGG TTATGTAGAA
    GATGGAAGAG AGATTTTTGA TGATGACTTA GAAGACAATG CTCTTGCTGA TTCAGGTAAA
    AGAGCTAAGG GAGCACCCAA AGATAAATCT AATGTAAAAA AGTCATCGGT GTCGAAGCCA
    AATAATATCA AGTCAATGTT TATGGCCAGT GCTGTCAAAA GGACAACAGA TAAAACTGTG
    GATCTGTCAA AAGATGACTT GCTTGGTGAT TTACTGCAAG ATTTAAAGTC ACAGGCTCTT
    CCTATAACAC CACCCCCAGT AATTACTCTA AAGAAGAAGA AACTTGCAGG GTCTCCTTTA
    AACCCTTTCT CGGTACCACC TGCTGCCCCA AAGGTTTTAC CTGTGAAAAA ACCACCAGCT
    TTAGCAAAGC TTGGGCATCT CTCAAGTGCT GCGCCATCTA ACAACTCTGC CAGTTCTTTT
    GTACCCCGGG AAATTAAGAA TGAGCCAAAT TCTGATTTGA TTTCTTCTGC TCCTTTGAAT
    GTGAAAACTC AAGTAAAAGA AGAGGATACG TCAATGGTTG AGTTCGATGA TGGAGATTTT
    GATGAACCAA TGGAAGAAGA CAATTTGGAA TCTCCTTCAG TTGTTTCTAG CACAATTAAG
    AAGGAAGAAC CAAAAATTAA GTGTGTGAAA GAGGATAGTG TTAAAATGGA GGAAAGTTCT
    TTCATGACTT CCGCTGCTTT AAATGAATCT TGTTGGGATC AAATTGATGA GGTTGAATCA
    ATGACCACTG AAGTTCAGGT AGATTCAAGC CGCCTACCCT TGGTTACAGG TGCAGATGGA
    AGTCAGGTGT TTAAGTTTTA TTGGCTAGAT GCCTATGAAG ACCAGTACAG TCAGCCAGGA
    GTAGTCTACT TATTTGGAAA AGTTTGGATA GAGACAGCTG AGGCGTATGT CAGCTGCTGT
    GTTAGTGTGA AGAACATTGA GAGGACTGTA TATCTCCTAC CTCGTGAAAA TAGAGTGCAG
    CTTTCTACAG GCAAAGACAC TGGAGTACCA GTGTCTATGA TGAATGTATA CCAGGAGTTT
    AATGAAAGTG TTGCAGAAAA GTACAAAATC ATGAAGTTCA AGTCTAAGAA AGTTGAGAAG
    GATTATGCTT TTGAAATTCC TGATGTTCCA GCCAGCTCTG AATATCTAGA GGTTCGATAT
    TCTGCGGACT CACCTCAACT TCCTCAGGAT CTAAAAGGAG AAACATTTTC ACATGTGTTT
    GGAACAAATA CTTCAAGCCT GGAGCTTTTC TTGCTTAGCA GAAAAATAAA AGGGCCCAGC
    TGGCTGGAGA TAAAAAGCCC ACAGCTGAGC AGTCAGCCCA TGAGCTGGTG CAAGGTTGAG
    GCAGTGGTGA TGAGGCCTGA CCAAGTGAAT GTTGTGAAGG ATCTGGCACC TCCACCTGTT
    GTTGTTTTGT CGCTCAGTAT GAAGACTGTG CAGAATGCAA AGACTCACCA AAATGAGATT
    GTGGCAATGG CTGCTTTGGT TCATCACAAA TTTCCCTTTG ATAAAGCACC ACCACAGCCA
    CCATTCCAGA CACATTTTTG TGTTCTGAGC AAACCTAATG ATTGCATTTT CCCATACGAC
    TTTAAGGAAG CGGTGAAACG AAAGAATGCA AATATTGAGA TTGCGTTAAC TGAACGAACT
    CTACTGGGCT TTTTCCTAGC AAAAATTCAT AAAATTGATC CAGATATTAT TGTAGGCCAT
    GACATTTATG GTTTTGATCT CGAAGTGTTG CTTCAAAGAA TTAGCTCGTG CAAGGTTCCT
    TTCTGGTCCA AAATCGGTAG ACTGAGAAGA TCTGTTATGC CAAAACTTGG GGGTCGAAGT
    GGCTTTGCAG AAAGGAATGC AGCTTGTGGT CGCATCATTT GCGATACTGA GATTTCTGCC
    AAGGAGTTAA TTCGATGTAA AAGCTATCAT TTGTCAGAAC TGGTACATCA AATATTGAAA
    ACAGAGAGAG TTTTGATGCC ACCAGAAAAC ATCCGCAATG CATACAGTGA TTCAGCACAT
    CTTCTTTACA TGCTGGAGAA CACCTGGACT GATGCAAAAT TCATCCTTCA GATCATGTGT
    GAGCTGAATG TTCTTCCCCT GGCCTTGCAG ATCACAAATA TTGCAGGCAA TGTCTTGTCA
    AGGACATTAA TGGGTGGACG CTCAGAAAGA AACGAGTACC TTCTCCTTCA TGCCTTCACA
    GAAAATAACT TCATAGTGCC AGACAAGCCA GTGTTCAAGA AAATTCAACA GACCACTGTG
    GAGGACGATG ATAATATGGA CACAGATCAA AATAAAAATA AGAAAGGTCG AAAGAAGGCT
    GCCTATGCTG GAGGATTGGT CCTGGAACCC AAAGTTGGTT TCTACGACAA ATTCATCTTG
    CTTCTGGATT TCAACAGCTT GTACCCCTCT ATCATTCAGG AATATAATAT CTGCTTTACA
    ACAGTGCAAA GAGAGGCTCC TACTTCACAG AAAGGTGAAG ATCAGGAAGA AATTCCAGAG
    CTACCTCATT CTGATCTTGA AATGGGGATC TTACCACGAG AGATACGGAA ACTGGTGGAG
    AGGAGGCGTC ACGTGAAGCA GTTAATGAAG CAGCCTGACC TAAACCCTGA TTTATACTTA
    CAGTATGACA TCAGACAAAA AGCTTTAAAG CTCACGGCAA ACAGCATGTA TGGATGCCTG
    GGCTTCTCCT ACAGCAGGTT CTATGCCAAA CCTTTGGCTG CATTGGTTAC TCACCAAGGG
    AGAGAGATTT TGCTGCATAC CAAGGAAATG GTACAGAAGA TGAATCTGGA AGTCATATAT
    GGAGACACGG ATTCTATCAT GATAAACACC AATTGTAATA ATTTAGAAGA AGTCTTCAAG
    CTTGGAAACC GGGTGAAAAG TGAGGTAAAC AAATCATACA AACTTTTAGA GATTGACATT
    GATGGTATCT TTAAATCACT CTTGCTACTT AAGAAAAAGA AGTATGCCGC TCTGACTGTG
    GAGCCTACAG GTGATGGAAA GTATGTTACT AAGCAGGAGC TTAAAGGTTT GGATATTGTC
    AGAAGAGACT GGTGTGAACT TGCTAAAGAA GCTGGAAATT ATGTTATCAG TCAGATTCTG
    TCTGATCAGC CACGTGACAA TATTGTTGAG AACATTCAGA AGAAATTAAC TGAAATTGGG
    GAGAATGTAA CAAATGGAAC TGTGCCCATT AGCCAGTATG AAATAAACAA GGCATTGACA
    AAGGATCCTC AGGATTACCC TGATAAAAAA AGCTTACCTC ACGTACATGT TGCCATGTGG
    ATAAATTCCC AAGGTGGAAG AAAGGTGAAA GCTGGTGATA CAATATCCTA TGTCATTTGC
    CAGGACGGAT CAAATCTTAG TGCCAGCCAG AGAGCATATG CTCCAGAGCA GCTGCAAAAA
    CAAGAGAACC TTTCCATTGA CACACAATAC TACCTGTCAC AGCAAGTCCA CCCAGTAGTG
    GCTCGGATCT GTGAGCCCAT TGATGGAATT GATTCTGCAC TCATTGCAAT GTGGTTAGGA
    CTTGACCCCT CCCAGTTCCG GGCTCACCGC CACCATCAGC AGGATGAGGA AAATGATGCA
    TTGTTGGGAG GTCCTTCCCA ACTCACAGAT GAGGAAAAAT ACAGAGATTG TGAACGATTC
    AAATTCTTCT GCCCAAAGTG TGGAACAGAG AACATTTATG ACAATGTTTT TGATGGCTCT
    GGGCTGCAGA TTGAACCCGG CTTGAAACGC TGCAGTAAAC CAGAGTGTGA TGCTTCTCCC
    TTGGACTACG TTATTCAGGT TCAAAACAAG CTGCTCTTGG ACATAAGACG TTACATAAAG
    AAATACTACA GTGGTTGGCT TGTATGTGAA GAAAAAACCT GCCAGAACAG AACACGACGA
    GTTCCTCTGA GCTTCTCCCG CAATGGGCCG ATATGCCAAG CTTGCACTAA AGCAACGCTA
    AGATCAGAGT ATCCGGAAAA GGCCCTGTAC ACTCAGCTTT GTTTCTATAG ATTCATTTTT
    GACTGGGATT ATGCACTGGA GAAAGTTGTT TCTGAACAAG AGAGAGGTCA CTTAAAAAAG
    AAGCATTTTC AAGAGTCAGA AAATCTGTAC AAGAAGTTAA AGAGCACTGT CGACCAAGTA
    CTTTCACAGA GCGGGTATTC AGAAGTGAAC CTCAGCAAGC TGTTCCAAAC ACTCAGCACC
    ATTAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa05773
    Clone ID Related Accession (Same CDS sequence) XM_002933612.4 , XM_004911754.3
    Accession Version XM_004911754.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4389bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA polymerase alpha catalytic subunit isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004911754.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCAGAAT CTGGAAGTTT TGCAGCATCA CGTTCGCGGC GTGAAAAAAC AGAAAAGAGC 
    GGCCGTAAAG AAGCATTGGA ACGTTTGAAA AGAGCAAAAG CTGGTGAAAA AGTCAAATAT
    GAGGTGGAAG AAATTTCAAG CATTTATGAG GAGGTGGATG AAGAACAATA CTCTAAAATT
    GTTCGTGACC GACAGGATGA TGATTGGATA GTGGATGATG ATGGGACCGG TTATGTAGAA
    GATGGAAGAG AGATTTTTGA TGATGACTTA GAAGACAATG CTCTTGCTGA TTCAGGTAAA
    AGAGCTAAGG GAGCACCCAA AGATAAATCT AATGTAAAAA AGTCATCGGT GTCGAAGCCA
    AATAATATCA AGTCAATGTT TATGGCCAGT GCTGTCAAAA GGACAACAGA TAAAACTGTG
    GATCTGTCAA AAGATGACTT GCTTGGTGAT TTACTGCAAG ATTTAAAGTC ACAGGCTCTT
    CCTATAACAC CACCCCCAGT AATTACTCTA AAGAAGAAGA AACTTGCAGG GTCTCCTTTA
    AACCCTTTCT CGGTACCACC TGCTGCCCCA AAGGTTTTAC CTGTGAAAAA ACCACCAGCT
    TTAGCAAAGC TTGGGCATCT CTCAAGTGCT GCGCCATCTA ACAACTCTGC CAGTTCTTTT
    GTACCCCGGG AAATTAAGAA TGAGCCAAAT TCTGATTTGA TTTCTTCTGC TCCTTTGAAT
    GTGAAAACTC AAGTAAAAGA AGAGGATACG TCAATGGTTG AGTTCGATGA TGGAGATTTT
    GATGAACCAA TGGAAGAAGA CAATTTGGAA TCTCCTTCAG TTGTTTCTAG CACAATTAAG
    AAGGAAGAAC CAAAAATTAA GTGTGTGAAA GAGGATAGTG TTAAAATGGA GGAAAGTTCT
    TTCATGACTT CCGCTGCTTT AAATGAATCT TGTTGGGATC AAATTGATGA GGTTGAATCA
    ATGACCACTG AAGTTCAGGT AGATTCAAGC CGCCTACCCT TGGTTACAGG TGCAGATGGA
    AGTCAGGTGT TTAAGTTTTA TTGGCTAGAT GCCTATGAAG ACCAGTACAG TCAGCCAGGA
    GTAGTCTACT TATTTGGAAA AGTTTGGATA GAGACAGCTG AGGCGTATGT CAGCTGCTGT
    GTTAGTGTGA AGAACATTGA GAGGACTGTA TATCTCCTAC CTCGTGAAAA TAGAGTGCAG
    CTTTCTACAG GCAAAGACAC TGGAGTACCA GTGTCTATGA TGAATGTATA CCAGGAGTTT
    AATGAAAGTG TTGCAGAAAA GTACAAAATC ATGAAGTTCA AGTCTAAGAA AGTTGAGAAG
    GATTATGCTT TTGAAATTCC TGATGTTCCA GCCAGCTCTG AATATCTAGA GGTTCGATAT
    TCTGCGGACT CACCTCAACT TCCTCAGGAT CTAAAAGGAG AAACATTTTC ACATGTGTTT
    GGAACAAATA CTTCAAGCCT GGAGCTTTTC TTGCTTAGCA GAAAAATAAA AGGGCCCAGC
    TGGCTGGAGA TAAAAAGCCC ACAGCTGAGC AGTCAGCCCA TGAGCTGGTG CAAGGTTGAG
    GCAGTGGTGA TGAGGCCTGA CCAAGTGAAT GTTGTGAAGG ATCTGGCACC TCCACCTGTT
    GTTGTTTTGT CGCTCAGTAT GAAGACTGTG CAGAATGCAA AGACTCACCA AAATGAGATT
    GTGGCAATGG CTGCTTTGGT TCATCACAAA TTTCCCTTTG ATAAAGCACC ACCACAGCCA
    CCATTCCAGA CACATTTTTG TGTTCTGAGC AAACCTAATG ATTGCATTTT CCCATACGAC
    TTTAAGGAAG CGGTGAAACG AAAGAATGCA AATATTGAGA TTGCGTTAAC TGAACGAACT
    CTACTGGGCT TTTTCCTAGC AAAAATTCAT AAAATTGATC CAGATATTAT TGTAGGCCAT
    GACATTTATG GTTTTGATCT CGAAGTGTTG CTTCAAAGAA TTAGCTCGTG CAAGGTTCCT
    TTCTGGTCCA AAATCGGTAG ACTGAGAAGA TCTGTTATGC CAAAACTTGG GGGTCGAAGT
    GGCTTTGCAG AAAGGAATGC AGCTTGTGGT CGCATCATTT GCGATACTGA GATTTCTGCC
    AAGGAGTTAA TTCGATGTAA AAGCTATCAT TTGTCAGAAC TGGTACATCA AATATTGAAA
    ACAGAGAGAG TTTTGATGCC ACCAGAAAAC ATCCGCAATG CATACAGTGA TTCAGCACAT
    CTTCTTTACA TGCTGGAGAA CACCTGGACT GATGCAAAAT TCATCCTTCA GATCATGTGT
    GAGCTGAATG TTCTTCCCCT GGCCTTGCAG ATCACAAATA TTGCAGGCAA TGTCTTGTCA
    AGGACATTAA TGGGTGGACG CTCAGAAAGA AACGAGTACC TTCTCCTTCA TGCCTTCACA
    GAAAATAACT TCATAGTGCC AGACAAGCCA GTGTTCAAGA AAATTCAACA GACCACTGTG
    GAGGACGATG ATAATATGGA CACAGATCAA AATAAAAATA AGAAAGGTCG AAAGAAGGCT
    GCCTATGCTG GAGGATTGGT CCTGGAACCC AAAGTTGGTT TCTACGACAA ATTCATCTTG
    CTTCTGGATT TCAACAGCTT GTACCCCTCT ATCATTCAGG AATATAATAT CTGCTTTACA
    ACAGTGCAAA GAGAGGCTCC TACTTCACAG AAAGGTGAAG ATCAGGAAGA AATTCCAGAG
    CTACCTCATT CTGATCTTGA AATGGGGATC TTACCACGAG AGATACGGAA ACTGGTGGAG
    AGGAGGCGTC ACGTGAAGCA GTTAATGAAG CAGCCTGACC TAAACCCTGA TTTATACTTA
    CAGTATGACA TCAGACAAAA AGCTTTAAAG CTCACGGCAA ACAGCATGTA TGGATGCCTG
    GGCTTCTCCT ACAGCAGGTT CTATGCCAAA CCTTTGGCTG CATTGGTTAC TCACCAAGGG
    AGAGAGATTT TGCTGCATAC CAAGGAAATG GTACAGAAGA TGAATCTGGA AGTCATATAT
    GGAGACACGG ATTCTATCAT GATAAACACC AATTGTAATA ATTTAGAAGA AGTCTTCAAG
    CTTGGAAACC GGGTGAAAAG TGAGGTAAAC AAATCATACA AACTTTTAGA GATTGACATT
    GATGGTATCT TTAAATCACT CTTGCTACTT AAGAAAAAGA AGTATGCCGC TCTGACTGTG
    GAGCCTACAG GTGATGGAAA GTATGTTACT AAGCAGGAGC TTAAAGGTTT GGATATTGTC
    AGAAGAGACT GGTGTGAACT TGCTAAAGAA GCTGGAAATT ATGTTATCAG TCAGATTCTG
    TCTGATCAGC CACGTGACAA TATTGTTGAG AACATTCAGA AGAAATTAAC TGAAATTGGG
    GAGAATGTAA CAAATGGAAC TGTGCCCATT AGCCAGTATG AAATAAACAA GGCATTGACA
    AAGGATCCTC AGGATTACCC TGATAAAAAA AGCTTACCTC ACGTACATGT TGCCATGTGG
    ATAAATTCCC AAGGTGGAAG AAAGGTGAAA GCTGGTGATA CAATATCCTA TGTCATTTGC
    CAGGACGGAT CAAATCTTAG TGCCAGCCAG AGAGCATATG CTCCAGAGCA GCTGCAAAAA
    CAAGAGAACC TTTCCATTGA CACACAATAC TACCTGTCAC AGCAAGTCCA CCCAGTAGTG
    GCTCGGATCT GTGAGCCCAT TGATGGAATT GATTCTGCAC TCATTGCAAT GTGGTTAGGA
    CTTGACCCCT CCCAGTTCCG GGCTCACCGC CACCATCAGC AGGATGAGGA AAATGATGCA
    TTGTTGGGAG GTCCTTCCCA ACTCACAGAT GAGGAAAAAT ACAGAGATTG TGAACGATTC
    AAATTCTTCT GCCCAAAGTG TGGAACAGAG AACATTTATG ACAATGTTTT TGATGGCTCT
    GGGCTGCAGA TTGAACCCGG CTTGAAACGC TGCAGTAAAC CAGAGTGTGA TGCTTCTCCC
    TTGGACTACG TTATTCAGGT TCAAAACAAG CTGCTCTTGG ACATAAGACG TTACATAAAG
    AAATACTACA GTGGTTGGCT TGTATGTGAA GAAAAAACCT GCCAGAACAG AACACGACGA
    GTTCCTCTGA GCTTCTCCCG CAATGGGCCG ATATGCCAAG CTTGCACTAA AGCAACGCTA
    AGATCAGAGT ATCCGGAAAA GGCCCTGTAC ACTCAGCTTT GTTTCTATAG ATTCATTTTT
    GACTGGGATT ATGCACTGGA GAAAGTTGTT TCTGAACAAG AGAGAGGTCA CTTAAAAAAG
    AAGCATTTTC AAGAGTCAGA AAATCTGTAC AAGAAGTTAA AGAGCACTGT CGACCAAGTA
    CTTTCACAGA GCGGGTATTC AGAAGTGAAC CTCAGCAAGC TGTTCCAAAC ACTCAGCACC
    ATTAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004911811.1
    CDS166..4554
    Translation

    Target ORF information:

    RefSeq Version XM_004911754.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis polymerase (DNA directed), alpha 1, catalytic subunit (pola1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004911754.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCAGAAT CTGGAAGTTT TGCAGCATCA CGTTCGCGGC GTGAAAAAAC AGAAAAGAGC 
    GGCCGTAAAG AAGCATTGGA ACGTTTGAAA AGAGCAAAAG CTGGTGAAAA AGTCAAATAT
    GAGGTGGAAG AAATTTCAAG CATTTATGAG GAGGTGGATG AAGAACAATA CTCTAAAATT
    GTTCGTGACC GACAGGATGA TGATTGGATA GTGGATGATG ATGGGACCGG TTATGTAGAA
    GATGGAAGAG AGATTTTTGA TGATGACTTA GAAGACAATG CTCTTGCTGA TTCAGGTAAA
    AGAGCTAAGG GAGCACCCAA AGATAAATCT AATGTAAAAA AGTCATCGGT GTCGAAGCCA
    AATAATATCA AGTCAATGTT TATGGCCAGT GCTGTCAAAA GGACAACAGA TAAAACTGTG
    GATCTGTCAA AAGATGACTT GCTTGGTGAT TTACTGCAAG ATTTAAAGTC ACAGGCTCTT
    CCTATAACAC CACCCCCAGT AATTACTCTA AAGAAGAAGA AACTTGCAGG GTCTCCTTTA
    AACCCTTTCT CGGTACCACC TGCTGCCCCA AAGGTTTTAC CTGTGAAAAA ACCACCAGCT
    TTAGCAAAGC TTGGGCATCT CTCAAGTGCT GCGCCATCTA ACAACTCTGC CAGTTCTTTT
    GTACCCCGGG AAATTAAGAA TGAGCCAAAT TCTGATTTGA TTTCTTCTGC TCCTTTGAAT
    GTGAAAACTC AAGTAAAAGA AGAGGATACG TCAATGGTTG AGTTCGATGA TGGAGATTTT
    GATGAACCAA TGGAAGAAGA CAATTTGGAA TCTCCTTCAG TTGTTTCTAG CACAATTAAG
    AAGGAAGAAC CAAAAATTAA GTGTGTGAAA GAGGATAGTG TTAAAATGGA GGAAAGTTCT
    TTCATGACTT CCGCTGCTTT AAATGAATCT TGTTGGGATC AAATTGATGA GGTTGAATCA
    ATGACCACTG AAGTTCAGGT AGATTCAAGC CGCCTACCCT TGGTTACAGG TGCAGATGGA
    AGTCAGGTGT TTAAGTTTTA TTGGCTAGAT GCCTATGAAG ACCAGTACAG TCAGCCAGGA
    GTAGTCTACT TATTTGGAAA AGTTTGGATA GAGACAGCTG AGGCGTATGT CAGCTGCTGT
    GTTAGTGTGA AGAACATTGA GAGGACTGTA TATCTCCTAC CTCGTGAAAA TAGAGTGCAG
    CTTTCTACAG GCAAAGACAC TGGAGTACCA GTGTCTATGA TGAATGTATA CCAGGAGTTT
    AATGAAAGTG TTGCAGAAAA GTACAAAATC ATGAAGTTCA AGTCTAAGAA AGTTGAGAAG
    GATTATGCTT TTGAAATTCC TGATGTTCCA GCCAGCTCTG AATATCTAGA GGTTCGATAT
    TCTGCGGACT CACCTCAACT TCCTCAGGAT CTAAAAGGAG AAACATTTTC ACATGTGTTT
    GGAACAAATA CTTCAAGCCT GGAGCTTTTC TTGCTTAGCA GAAAAATAAA AGGGCCCAGC
    TGGCTGGAGA TAAAAAGCCC ACAGCTGAGC AGTCAGCCCA TGAGCTGGTG CAAGGTTGAG
    GCAGTGGTGA TGAGGCCTGA CCAAGTGAAT GTTGTGAAGG ATCTGGCACC TCCACCTGTT
    GTTGTTTTGT CGCTCAGTAT GAAGACTGTG CAGAATGCAA AGACTCACCA AAATGAGATT
    GTGGCAATGG CTGCTTTGGT TCATCACAAA TTTCCCTTTG ATAAAGCACC ACCACAGCCA
    CCATTCCAGA CACATTTTTG TGTTCTGAGC AAACCTAATG ATTGCATTTT CCCATACGAC
    TTTAAGGAAG CGGTGAAACG AAAGAATGCA AATATTGAGA TTGCGTTAAC TGAACGAACT
    CTACTGGGCT TTTTCCTAGC AAAAATTCAT AAAATTGATC CAGATATTAT TGTAGGCCAT
    GACATTTATG GTTTTGATCT CGAAGTGTTG CTTCAAAGAA TTAGCTCGTG CAAGGTTCCT
    TTCTGGTCCA AAATCGGTAG ACTGAGAAGA TCTGTTATGC CAAAACTTGG GGGTCGAAGT
    GGCTTTGCAG AAAGGAATGC AGCTTGTGGT CGCATCATTT GCGATACTGA GATTTCTGCC
    AAGGAGTTAA TTCGATGTAA AAGCTATCAT TTGTCAGAAC TGGTACATCA AATATTGAAA
    ACAGAGAGAG TTTTGATGCC ACCAGAAAAC ATCCGCAATG CATACAGTGA TTCAGCACAT
    CTTCTTTACA TGCTGGAGAA CACCTGGACT GATGCAAAAT TCATCCTTCA GATCATGTGT
    GAGCTGAATG TTCTTCCCCT GGCCTTGCAG ATCACAAATA TTGCAGGCAA TGTCTTGTCA
    AGGACATTAA TGGGTGGACG CTCAGAAAGA AACGAGTACC TTCTCCTTCA TGCCTTCACA
    GAAAATAACT TCATAGTGCC AGACAAGCCA GTGTTCAAGA AAATTCAACA GACCACTGTG
    GAGGACGATG ATAATATGGA CACAGATCAA AATAAAAATA AGAAAGGTCG AAAGAAGGCT
    GCCTATGCTG GAGGATTGGT CCTGGAACCC AAAGTTGGTT TCTACGACAA ATTCATCTTG
    CTTCTGGATT TCAACAGCTT GTACCCCTCT ATCATTCAGG AATATAATAT CTGCTTTACA
    ACAGTGCAAA GAGAGGCTCC TACTTCACAG AAAGGTGAAG ATCAGGAAGA AATTCCAGAG
    CTACCTCATT CTGATCTTGA AATGGGGATC TTACCACGAG AGATACGGAA ACTGGTGGAG
    AGGAGGCGTC ACGTGAAGCA GTTAATGAAG CAGCCTGACC TAAACCCTGA TTTATACTTA
    CAGTATGACA TCAGACAAAA AGCTTTAAAG CTCACGGCAA ACAGCATGTA TGGATGCCTG
    GGCTTCTCCT ACAGCAGGTT CTATGCCAAA CCTTTGGCTG CATTGGTTAC TCACCAAGGG
    AGAGAGATTT TGCTGCATAC CAAGGAAATG GTACAGAAGA TGAATCTGGA AGTCATATAT
    GGAGACACGG ATTCTATCAT GATAAACACC AATTGTAATA ATTTAGAAGA AGTCTTCAAG
    CTTGGAAACC GGGTGAAAAG TGAGGTAAAC AAATCATACA AACTTTTAGA GATTGACATT
    GATGGTATCT TTAAATCACT CTTGCTACTT AAGAAAAAGA AGTATGCCGC TCTGACTGTG
    GAGCCTACAG GTGATGGAAA GTATGTTACT AAGCAGGAGC TTAAAGGTTT GGATATTGTC
    AGAAGAGACT GGTGTGAACT TGCTAAAGAA GCTGGAAATT ATGTTATCAG TCAGATTCTG
    TCTGATCAGC CACGTGACAA TATTGTTGAG AACATTCAGA AGAAATTAAC TGAAATTGGG
    GAGAATGTAA CAAATGGAAC TGTGCCCATT AGCCAGTATG AAATAAACAA GGCATTGACA
    AAGGATCCTC AGGATTACCC TGATAAAAAA AGCTTACCTC ACGTACATGT TGCCATGTGG
    ATAAATTCCC AAGGTGGAAG AAAGGTGAAA GCTGGTGATA CAATATCCTA TGTCATTTGC
    CAGGACGGAT CAAATCTTAG TGCCAGCCAG AGAGCATATG CTCCAGAGCA GCTGCAAAAA
    CAAGAGAACC TTTCCATTGA CACACAATAC TACCTGTCAC AGCAAGTCCA CCCAGTAGTG
    GCTCGGATCT GTGAGCCCAT TGATGGAATT GATTCTGCAC TCATTGCAAT GTGGTTAGGA
    CTTGACCCCT CCCAGTTCCG GGCTCACCGC CACCATCAGC AGGATGAGGA AAATGATGCA
    TTGTTGGGAG GTCCTTCCCA ACTCACAGAT GAGGAAAAAT ACAGAGATTG TGAACGATTC
    AAATTCTTCT GCCCAAAGTG TGGAACAGAG AACATTTATG ACAATGTTTT TGATGGCTCT
    GGGCTGCAGA TTGAACCCGG CTTGAAACGC TGCAGTAAAC CAGAGTGTGA TGCTTCTCCC
    TTGGACTACG TTATTCAGGT TCAAAACAAG CTGCTCTTGG ACATAAGACG TTACATAAAG
    AAATACTACA GTGGTTGGCT TGTATGTGAA GAAAAAACCT GCCAGAACAG AACACGACGA
    GTTCCTCTGA GCTTCTCCCG CAATGGGCCG ATATGCCAAG CTTGCACTAA AGCAACGCTA
    AGATCAGAGT ATCCGGAAAA GGCCCTGTAC ACTCAGCTTT GTTTCTATAG ATTCATTTTT
    GACTGGGATT ATGCACTGGA GAAAGTTGTT TCTGAACAAG AGAGAGGTCA CTTAAAAAAG
    AAGCATTTTC AAGAGTCAGA AAATCTGTAC AAGAAGTTAA AGAGCACTGT CGACCAAGTA
    CTTTCACAGA GCGGGTATTC AGAAGTGAAC CTCAGCAAGC TGTTCCAAAC ACTCAGCACC
    ATTAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa33001
    Clone ID Related Accession (Same CDS sequence) XM_012957632.2
    Accession Version XM_012957632.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4443bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA polymerase alpha catalytic subunit isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957632.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCACTTGG TGTTCTGTGC GCCCCGCAGT ACCGTCTTCC CTCCTTACCT TGACATGTCA 
    GAATCTGGAA GTTTTGCAGC ATCACGTTCG CGGCGTGAAA AAACAGAAAA GAGCGGCCGT
    AAAGAAGCAT TGGAACGTTT GAAAAGAGCA AAAGCTGGTG AAAAAGTCAA ATATGAGGTG
    GAAGAAATTT CAAGCATTTA TGAGGAGGTG GATGAAGAAC AATACTCTAA AATTGTTCGT
    GACCGACAGG ATGATGATTG GATAGTGGAT GATGATGGGA CCGGTTATGT AGAAGATGGA
    AGAGAGATTT TTGATGATGA CTTAGAAGAC AATGCTCTTG CTGATTCAGG TAAAAGAGCT
    AAGGGAGCAC CCAAAGATAA ATCTAATGTA AAAAAGTCAT CGGTGTCGAA GCCAAATAAT
    ATCAAGTCAA TGTTTATGGC CAGTGCTGTC AAAAGGACAA CAGATAAAAC TGTGGATCTG
    TCAAAAGATG ACTTGCTTGG TGATTTACTG CAAGATTTAA AGTCACAGGC TCTTCCTATA
    ACACCACCCC CAGTAATTAC TCTAAAGAAG AAGAAACTTG CAGGGTCTCC TTTAAACCCT
    TTCTCGGTAC CACCTGCTGC CCCAAAGGTT TTACCTGTGA AAAAACCACC AGCTTTAGCA
    AAGCTTGGGC ATCTCTCAAG TGCTGCGCCA TCTAACAACT CTGCCAGTTC TTTTGTACCC
    CGGGAAATTA AGAATGAGCC AAATTCTGAT TTGATTTCTT CTGCTCCTTT GAATGTGAAA
    ACTCAAGTAA AAGAAGAGGA TACGTCAATG GTTGAGTTCG ATGATGGAGA TTTTGATGAA
    CCAATGGAAG AAGACAATTT GGAATCTCCT TCAGTTGTTT CTAGCACAAT TAAGAAGGAA
    GAACCAAAAA TTAAGTGTGT GAAAGAGGAT AGTGTTAAAA TGGAGGAAAG TTCTTTCATG
    ACTTCCGCTG CTTTAAATGA ATCTTGTTGG GATCAAATTG ATGAGGTTGA ATCAATGACC
    ACTGAAGTTC AGGTAGATTC AAGCCGCCTA CCCTTGGTTA CAGGTGCAGA TGGAAGTCAG
    GTGTTTAAGT TTTATTGGCT AGATGCCTAT GAAGACCAGT ACAGTCAGCC AGGAGTAGTC
    TACTTATTTG GAAAAGTTTG GATAGAGACA GCTGAGGCGT ATGTCAGCTG CTGTGTTAGT
    GTGAAGAACA TTGAGAGGAC TGTATATCTC CTACCTCGTG AAAATAGAGT GCAGCTTTCT
    ACAGGCAAAG ACACTGGAGT ACCAGTGTCT ATGATGAATG TATACCAGGA GTTTAATGAA
    AGTGTTGCAG AAAAGTACAA AATCATGAAG TTCAAGTCTA AGAAAGTTGA GAAGGATTAT
    GCTTTTGAAA TTCCTGATGT TCCAGCCAGC TCTGAATATC TAGAGGTTCG ATATTCTGCG
    GACTCACCTC AACTTCCTCA GGATCTAAAA GGAGAAACAT TTTCACATGT GTTTGGAACA
    AATACTTCAA GCCTGGAGCT TTTCTTGCTT AGCAGAAAAA TAAAAGGGCC CAGCTGGCTG
    GAGATAAAAA GCCCACAGCT GAGCAGTCAG CCCATGAGCT GGTGCAAGGT TGAGGCAGTG
    GTGATGAGGC CTGACCAAGT GAATGTTGTG AAGGATCTGG CACCTCCACC TGTTGTTGTT
    TTGTCGCTCA GTATGAAGAC TGTGCAGAAT GCAAAGACTC ACCAAAATGA GATTGTGGCA
    ATGGCTGCTT TGGTTCATCA CAAATTTCCC TTTGATAAAG CACCACCACA GCCACCATTC
    CAGACACATT TTTGTGTTCT GAGCAAACCT AATGATTGCA TTTTCCCATA CGACTTTAAG
    GAAGCGGTGA AACGAAAGAA TGCAAATATT GAGATTGCGT TAACTGAACG AACTCTACTG
    GGCTTTTTCC TAGCAAAAAT TCATAAAATT GATCCAGATA TTATTGTAGG CCATGACATT
    TATGGTTTTG ATCTCGAAGT GTTGCTTCAA AGAATTAGCT CGTGCAAGGT TCCTTTCTGG
    TCCAAAATCG GTAGACTGAG AAGATCTGTT ATGCCAAAAC TTGGGGGTCG AAGTGGCTTT
    GCAGAAAGGA ATGCAGCTTG TGGTCGCATC ATTTGCGATA CTGAGATTTC TGCCAAGGAG
    TTAATTCGAT GTAAAAGCTA TCATTTGTCA GAACTGGTAC ATCAAATATT GAAAACAGAG
    AGAGTTTTGA TGCCACCAGA AAACATCCGC AATGCATACA GTGATTCAGC ACATCTTCTT
    TACATGCTGG AGAACACCTG GACTGATGCA AAATTCATCC TTCAGATCAT GTGTGAGCTG
    AATGTTCTTC CCCTGGCCTT GCAGATCACA AATATTGCAG GCAATGTCTT GTCAAGGACA
    TTAATGGGTG GACGCTCAGA AAGAAACGAG TACCTTCTCC TTCATGCCTT CACAGAAAAT
    AACTTCATAG TGCCAGACAA GCCAGTGTTC AAGAAAATTC AACAGACCAC TGTGGAGGAC
    GATGATAATA TGGACACAGA TCAAAATAAA AATAAGAAAG GTCGAAAGAA GGCTGCCTAT
    GCTGGAGGAT TGGTCCTGGA ACCCAAAGTT GGTTTCTACG ACAAATTCAT CTTGCTTCTG
    GATTTCAACA GCTTGTACCC CTCTATCATT CAGGAATATA ATATCTGCTT TACAACAGTG
    CAAAGAGAGG CTCCTACTTC ACAGAAAGGT GAAGATCAGG AAGAAATTCC AGAGCTACCT
    CATTCTGATC TTGAAATGGG GATCTTACCA CGAGAGATAC GGAAACTGGT GGAGAGGAGG
    CGTCACGTGA AGCAGTTAAT GAAGCAGCCT GACCTAAACC CTGATTTATA CTTACAGTAT
    GACATCAGAC AAAAAGCTTT AAAGCTCACG GCAAACAGCA TGTATGGATG CCTGGGCTTC
    TCCTACAGCA GGTTCTATGC CAAACCTTTG GCTGCATTGG TTACTCACCA AGGGAGAGAG
    ATTTTGCTGC ATACCAAGGA AATGGTACAG AAGATGAATC TGGAAGTCAT ATATGGAGAC
    ACGGATTCTA TCATGATAAA CACCAATTGT AATAATTTAG AAGAAGTCTT CAAGCTTGGA
    AACCGGGTGA AAAGTGAGGT AAACAAATCA TACAAACTTT TAGAGATTGA CATTGATGGT
    ATCTTTAAAT CACTCTTGCT ACTTAAGAAA AAGAAGTATG CCGCTCTGAC TGTGGAGCCT
    ACAGGTGATG GAAAGTATGT TACTAAGCAG GAGCTTAAAG GTTTGGATAT TGTCAGAAGA
    GACTGGTGTG AACTTGCTAA AGAAGCTGGA AATTATGTTA TCAGTCAGAT TCTGTCTGAT
    CAGCCACGTG ACAATATTGT TGAGAACATT CAGAAGAAAT TAACTGAAAT TGGGGAGAAT
    GTAACAAATG GAACTGTGCC CATTAGCCAG TATGAAATAA ACAAGGCATT GACAAAGGAT
    CCTCAGGATT ACCCTGATAA AAAAAGCTTA CCTCACGTAC ATGTTGCCAT GTGGATAAAT
    TCCCAAGGTG GAAGAAAGGT GAAAGCTGGT GATACAATAT CCTATGTCAT TTGCCAGGAC
    GGATCAAATC TTAGTGCCAG CCAGAGAGCA TATGCTCCAG AGCAGCTGCA AAAACAAGAG
    AACCTTTCCA TTGACACACA ATACTACCTG TCACAGCAAG TCCACCCAGT AGTGGCTCGG
    ATCTGTGAGC CCATTGATGG AATTGATTCT GCACTCATTG CAATGTGGTT AGGACTTGAC
    CCCTCCCAGT TCCGGGCTCA CCGCCACCAT CAGCAGGATG AGGAAAATGA TGCATTGTTG
    GGAGGTCCTT CCCAACTCAC AGATGAGGAA AAATACAGAG ATTGTGAACG ATTCAAATTC
    TTCTGCCCAA AGTGTGGAAC AGAGAACATT TATGACAATG TTTTTGATGG CTCTGGGCTG
    CAGATTGAAC CCGGCTTGAA ACGCTGCAGT AAACCAGAGT GTGATGCTTC TCCCTTGGAC
    TACGTTATTC AGGTTCAAAA CAAGCTGCTC TTGGACATAA GACGTTACAT AAAGAAATAC
    TACAGTGGTT GGCTTGTATG TGAAGAAAAA ACCTGCCAGA ACAGAACACG ACGAGTTCCT
    CTGAGCTTCT CCCGCAATGG GCCGATATGC CAAGCTTGCA CTAAAGCAAC GCTAAGATCA
    GAGTATCCGG AAAAGGCCCT GTACACTCAG CTTTGTTTCT ATAGATTCAT TTTTGACTGG
    GATTATGCAC TGGAGAAAGT TGTTTCTGAA CAAGAGAGAG GTCACTTAAA AAAGAAGCAT
    TTTCAAGAGT CAGAAAATCT GTACAAGAAG TTAAAGAGCA CTGTCGACCA AGTACTTTCA
    CAGAGCGGGT ATTCAGAAGT GAACCTCAGC AAGCTGTTCC AAACACTCAG CACCATTAAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813086.1
    CDS48..4490
    Translation

    Target ORF information:

    RefSeq Version XM_012957632.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis polymerase (DNA directed), alpha 1, catalytic subunit (pola1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957632.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCACTTGG TGTTCTGTGC GCCCCGCAGT ACCGTCTTCC CTCCTTACCT TGACATGTCA 
    GAATCTGGAA GTTTTGCAGC ATCACGTTCG CGGCGTGAAA AAACAGAAAA GAGCGGCCGT
    AAAGAAGCAT TGGAACGTTT GAAAAGAGCA AAAGCTGGTG AAAAAGTCAA ATATGAGGTG
    GAAGAAATTT CAAGCATTTA TGAGGAGGTG GATGAAGAAC AATACTCTAA AATTGTTCGT
    GACCGACAGG ATGATGATTG GATAGTGGAT GATGATGGGA CCGGTTATGT AGAAGATGGA
    AGAGAGATTT TTGATGATGA CTTAGAAGAC AATGCTCTTG CTGATTCAGG TAAAAGAGCT
    AAGGGAGCAC CCAAAGATAA ATCTAATGTA AAAAAGTCAT CGGTGTCGAA GCCAAATAAT
    ATCAAGTCAA TGTTTATGGC CAGTGCTGTC AAAAGGACAA CAGATAAAAC TGTGGATCTG
    TCAAAAGATG ACTTGCTTGG TGATTTACTG CAAGATTTAA AGTCACAGGC TCTTCCTATA
    ACACCACCCC CAGTAATTAC TCTAAAGAAG AAGAAACTTG CAGGGTCTCC TTTAAACCCT
    TTCTCGGTAC CACCTGCTGC CCCAAAGGTT TTACCTGTGA AAAAACCACC AGCTTTAGCA
    AAGCTTGGGC ATCTCTCAAG TGCTGCGCCA TCTAACAACT CTGCCAGTTC TTTTGTACCC
    CGGGAAATTA AGAATGAGCC AAATTCTGAT TTGATTTCTT CTGCTCCTTT GAATGTGAAA
    ACTCAAGTAA AAGAAGAGGA TACGTCAATG GTTGAGTTCG ATGATGGAGA TTTTGATGAA
    CCAATGGAAG AAGACAATTT GGAATCTCCT TCAGTTGTTT CTAGCACAAT TAAGAAGGAA
    GAACCAAAAA TTAAGTGTGT GAAAGAGGAT AGTGTTAAAA TGGAGGAAAG TTCTTTCATG
    ACTTCCGCTG CTTTAAATGA ATCTTGTTGG GATCAAATTG ATGAGGTTGA ATCAATGACC
    ACTGAAGTTC AGGTAGATTC AAGCCGCCTA CCCTTGGTTA CAGGTGCAGA TGGAAGTCAG
    GTGTTTAAGT TTTATTGGCT AGATGCCTAT GAAGACCAGT ACAGTCAGCC AGGAGTAGTC
    TACTTATTTG GAAAAGTTTG GATAGAGACA GCTGAGGCGT ATGTCAGCTG CTGTGTTAGT
    GTGAAGAACA TTGAGAGGAC TGTATATCTC CTACCTCGTG AAAATAGAGT GCAGCTTTCT
    ACAGGCAAAG ACACTGGAGT ACCAGTGTCT ATGATGAATG TATACCAGGA GTTTAATGAA
    AGTGTTGCAG AAAAGTACAA AATCATGAAG TTCAAGTCTA AGAAAGTTGA GAAGGATTAT
    GCTTTTGAAA TTCCTGATGT TCCAGCCAGC TCTGAATATC TAGAGGTTCG ATATTCTGCG
    GACTCACCTC AACTTCCTCA GGATCTAAAA GGAGAAACAT TTTCACATGT GTTTGGAACA
    AATACTTCAA GCCTGGAGCT TTTCTTGCTT AGCAGAAAAA TAAAAGGGCC CAGCTGGCTG
    GAGATAAAAA GCCCACAGCT GAGCAGTCAG CCCATGAGCT GGTGCAAGGT TGAGGCAGTG
    GTGATGAGGC CTGACCAAGT GAATGTTGTG AAGGATCTGG CACCTCCACC TGTTGTTGTT
    TTGTCGCTCA GTATGAAGAC TGTGCAGAAT GCAAAGACTC ACCAAAATGA GATTGTGGCA
    ATGGCTGCTT TGGTTCATCA CAAATTTCCC TTTGATAAAG CACCACCACA GCCACCATTC
    CAGACACATT TTTGTGTTCT GAGCAAACCT AATGATTGCA TTTTCCCATA CGACTTTAAG
    GAAGCGGTGA AACGAAAGAA TGCAAATATT GAGATTGCGT TAACTGAACG AACTCTACTG
    GGCTTTTTCC TAGCAAAAAT TCATAAAATT GATCCAGATA TTATTGTAGG CCATGACATT
    TATGGTTTTG ATCTCGAAGT GTTGCTTCAA AGAATTAGCT CGTGCAAGGT TCCTTTCTGG
    TCCAAAATCG GTAGACTGAG AAGATCTGTT ATGCCAAAAC TTGGGGGTCG AAGTGGCTTT
    GCAGAAAGGA ATGCAGCTTG TGGTCGCATC ATTTGCGATA CTGAGATTTC TGCCAAGGAG
    TTAATTCGAT GTAAAAGCTA TCATTTGTCA GAACTGGTAC ATCAAATATT GAAAACAGAG
    AGAGTTTTGA TGCCACCAGA AAACATCCGC AATGCATACA GTGATTCAGC ACATCTTCTT
    TACATGCTGG AGAACACCTG GACTGATGCA AAATTCATCC TTCAGATCAT GTGTGAGCTG
    AATGTTCTTC CCCTGGCCTT GCAGATCACA AATATTGCAG GCAATGTCTT GTCAAGGACA
    TTAATGGGTG GACGCTCAGA AAGAAACGAG TACCTTCTCC TTCATGCCTT CACAGAAAAT
    AACTTCATAG TGCCAGACAA GCCAGTGTTC AAGAAAATTC AACAGACCAC TGTGGAGGAC
    GATGATAATA TGGACACAGA TCAAAATAAA AATAAGAAAG GTCGAAAGAA GGCTGCCTAT
    GCTGGAGGAT TGGTCCTGGA ACCCAAAGTT GGTTTCTACG ACAAATTCAT CTTGCTTCTG
    GATTTCAACA GCTTGTACCC CTCTATCATT CAGGAATATA ATATCTGCTT TACAACAGTG
    CAAAGAGAGG CTCCTACTTC ACAGAAAGGT GAAGATCAGG AAGAAATTCC AGAGCTACCT
    CATTCTGATC TTGAAATGGG GATCTTACCA CGAGAGATAC GGAAACTGGT GGAGAGGAGG
    CGTCACGTGA AGCAGTTAAT GAAGCAGCCT GACCTAAACC CTGATTTATA CTTACAGTAT
    GACATCAGAC AAAAAGCTTT AAAGCTCACG GCAAACAGCA TGTATGGATG CCTGGGCTTC
    TCCTACAGCA GGTTCTATGC CAAACCTTTG GCTGCATTGG TTACTCACCA AGGGAGAGAG
    ATTTTGCTGC ATACCAAGGA AATGGTACAG AAGATGAATC TGGAAGTCAT ATATGGAGAC
    ACGGATTCTA TCATGATAAA CACCAATTGT AATAATTTAG AAGAAGTCTT CAAGCTTGGA
    AACCGGGTGA AAAGTGAGGT AAACAAATCA TACAAACTTT TAGAGATTGA CATTGATGGT
    ATCTTTAAAT CACTCTTGCT ACTTAAGAAA AAGAAGTATG CCGCTCTGAC TGTGGAGCCT
    ACAGGTGATG GAAAGTATGT TACTAAGCAG GAGCTTAAAG GTTTGGATAT TGTCAGAAGA
    GACTGGTGTG AACTTGCTAA AGAAGCTGGA AATTATGTTA TCAGTCAGAT TCTGTCTGAT
    CAGCCACGTG ACAATATTGT TGAGAACATT CAGAAGAAAT TAACTGAAAT TGGGGAGAAT
    GTAACAAATG GAACTGTGCC CATTAGCCAG TATGAAATAA ACAAGGCATT GACAAAGGAT
    CCTCAGGATT ACCCTGATAA AAAAAGCTTA CCTCACGTAC ATGTTGCCAT GTGGATAAAT
    TCCCAAGGTG GAAGAAAGGT GAAAGCTGGT GATACAATAT CCTATGTCAT TTGCCAGGAC
    GGATCAAATC TTAGTGCCAG CCAGAGAGCA TATGCTCCAG AGCAGCTGCA AAAACAAGAG
    AACCTTTCCA TTGACACACA ATACTACCTG TCACAGCAAG TCCACCCAGT AGTGGCTCGG
    ATCTGTGAGC CCATTGATGG AATTGATTCT GCACTCATTG CAATGTGGTT AGGACTTGAC
    CCCTCCCAGT TCCGGGCTCA CCGCCACCAT CAGCAGGATG AGGAAAATGA TGCATTGTTG
    GGAGGTCCTT CCCAACTCAC AGATGAGGAA AAATACAGAG ATTGTGAACG ATTCAAATTC
    TTCTGCCCAA AGTGTGGAAC AGAGAACATT TATGACAATG TTTTTGATGG CTCTGGGCTG
    CAGATTGAAC CCGGCTTGAA ACGCTGCAGT AAACCAGAGT GTGATGCTTC TCCCTTGGAC
    TACGTTATTC AGGTTCAAAA CAAGCTGCTC TTGGACATAA GACGTTACAT AAAGAAATAC
    TACAGTGGTT GGCTTGTATG TGAAGAAAAA ACCTGCCAGA ACAGAACACG ACGAGTTCCT
    CTGAGCTTCT CCCGCAATGG GCCGATATGC CAAGCTTGCA CTAAAGCAAC GCTAAGATCA
    GAGTATCCGG AAAAGGCCCT GTACACTCAG CTTTGTTTCT ATAGATTCAT TTTTGACTGG
    GATTATGCAC TGGAGAAAGT TGTTTCTGAA CAAGAGAGAG GTCACTTAAA AAAGAAGCAT
    TTTCAAGAGT CAGAAAATCT GTACAAGAAG TTAAAGAGCA CTGTCGACCA AGTACTTTCA
    CAGAGCGGGT ATTCAGAAGT GAACCTCAGC AAGCTGTTCC AAACACTCAG CACCATTAAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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