eif4g1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

  • Gene

  • Clones

  • gRNAs

The following eif4g1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eif4g1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa10204 XM_002935144.5
Latest version!
Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa10204 XM_004914398.4
Latest version!
Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa10204 XM_012963377.3
Latest version!
Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa10205 XM_004914400.4
Latest version!
Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa10205 XM_004914401.4
Latest version!
Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa10205 XM_012963378.3
Latest version!
Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa10206 XM_004914402.4
Latest version!
Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa10204 XM_018094420.1
Latest version!
Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa10204 XM_018094419.1
Latest version!
Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
View Old Accession Versions >>
OXa10204 XM_004914398.3 Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa10205 XM_004914401.3 Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa10205 XM_012963378.2 Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa10204 XM_002935144.4 Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa10205 XM_004914400.3 Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa10204 XM_012963377.2 Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa10206 XM_004914402.3 Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa10204
    Clone ID Related Accession (Same CDS sequence) XM_018094420.1 , XM_004914398.4 , XM_004914398.3 , XM_002935144.5 , XM_018094419.1 , XM_012963377.3 , XM_002935144.4 , XM_012963377.2
    Accession Version XM_004914398.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4830bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004914398.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
    CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA
    CAAATGAATA CACAATCTCA GCCTCGACAG GCAGGATTCA GATCAATCCA GCATTATTAT
    CAAAATAGGA CACAGCCTCC CACTAGTGCC ACCAGAGTGC AGAGTGGTTC TTCTGCTCGA
    GCAGTTACTC CAGCCCATGT GTATCAACCA GGCTCCCAAG TTATGATGAT TCCTTCACAG
    ATTTCATACC CTTCCCAGGG ATACTACATT CAGGGACAGG GACGCTCATA TGTGGTGCCC
    ACAAACCAGT ACCCTGTGCA GACTGGGGCT CCAGGTTTCT TCCCCAGTGC AAGTCCCTCA
    GAATATGGCA CATATGCTGG AGCATATTAC CCTGCACAAC AGACGTTCCC TCCTGGAGTG
    CCTGGAGCAC AAGTTATGAT GAATCAGCCA CAGGTGATCC CTCCTAAACG GGAGCGCAAA
    ACGATAAGAA TTAGAGACCC CAACCAAGGA GGCAAAGATA TCACAGAAGA AATTCTCTCA
    GGAGGAAGAA CATCCTCTAC GCCAACGCCT CCACAGTCAA CTGTGGGAAG TTCTGAAATT
    CAAGCTAATG GAGAAAGCGT TCATCATGTT ACCGTTGTTG TGCGCCCAGA TGATGGACCC
    AAGGCCACCC AAGTGCTGAT CAAAGCCTCA GAGAGCCCTT CCCCTCCTCT GCCTCTCTCT
    GAAGCTGAGC CAAGCATAGC AAATGTGTGT GATATTTCAA AAGATAACAC TATCTCTGAG
    TTGACTTCTC ATATAGTAGA GCCCCTAAGT TCAGAAGAGA CAACCATTGA TACTTCTACC
    TCTCAGGTGG ATCTCAAACA CCAGCCAGTG GCTGCTATGG CAGAGCATGA AACTCTTATA
    GAGGAGGCAT CAATACAGGA GGTGGCCTTT GCAGAGGCAC CCATCGCTCA ATCAGAGATA
    CCAAAATCTC TTGCCTCCCA GCCTCTAGCA GAAATCTCTA CCCCTGAACA GATTTCTAAA
    CTTGACGCAC AAGTGCCATC TTTGCAACAA GAGGAGTTAC TTACAGAAAC TGTTGAACTT
    AATTTAAGCA ATCAGGCTCT TCCTTCACAA ATTTCTGAGC CTGAACCTTT TGAACCTGAG
    GCTTCAGCTT CAGATCCTCT ACATGAGGAG CCCACAGAGC CCACAGTTTC AACAGAAGAA
    CCACAAGCTA ATGGACTGTC TGAGGAGACA CCAGTATTAC CTGTACAGCC ACAACCAACA
    TCTGTTTGCC AGACTGAGCC CTCCCTAGAA TCCCCCATCG CTCAGCCTGA GGAGCTGCTT
    CTAACAAATG GAGGTGGACC ACCTGGCCTA CAAGAAAATG ATGCACCTGA TTCACAGGTT
    GAAGCTCATG TTAGCCCAAT CACTGAGCCT GAGGACCAAC CTGTGACAGT CTCTGATAAC
    GTGCTTGTGG AAGAGGATGT AGAAAAAACA TTGCCTGCGG ATACGGAAGT CGTGGCTTCA
    ACCCAAGTTG TAAAGCAGAG CTCCGAGAGT CAGTCTCAGG TTGCTGTTGC TGCACCCAAG
    AAAAAGCGAA AAATGAAGGA ACTAAACAAG AAAGAGGCAG TGGGAGATCT CCTTGATGCG
    TTCAAAGAGG CTGGTGATGG CTCTTCTGAT GTGGATTCTT CCTCTAAGCC TACCAGTGCT
    GCACCTGAAC CAGATCAGAG CCCTTGTGCT CCTCCTGAGG AACAGGATGA AACTTGGGAA
    GAGAAGGAAG ATAAACTGGA TGCAGAGAAT ATTAAACCAG GAGAACATAA ATATAAATAC
    AAAGAAGAGC AATGGAAACC CCTAAACCCG GAGGAAAAGA AGAGTTATGA TCGCGAGTTT
    CTGCTAGGGT TCCAGTTTAT TGTGGCCAGT ATGCAGAAAC CTGCGGGTCT TCCTCAAATT
    ACTGATGTGG TGCTGGATAA GGCAAACAAA ACTCCATTGC GACCATTGGA TCCTACACGT
    CTGACGGGAA TGAACTGTGG GCCAGATTTT ACACCTTCAT TTGCCAACCT TGGTCGGCCT
    GCTGTGGGTA ACCGTGGACC ACCAGCTGGC ATGGGTCCGA GACGGTCCCA GCAGGGTCCA
    CGAAAAGAGC CTCGTAAAAT TATTCAAACC GTATCATTAA ATGAGAATGT ACAACTAAAC
    AAAGCTGAAA AGGCATGGAA ACCGATAAAC AAGAGAGCTG GCGATGAGGA GGACCCAGAA
    AATATTAAGA CACAGGACCT ATTCCGAAGG GTTCGCAGTA TCTTGAATAA GCTGACCCCT
    CAGATGTTCC AGCAGCTGAT GAAGCAGGTC ACTGACCTTA CTATTGATAC CGAAGATCGT
    CTTAAGGGTG TCATTGACCT AATTTTTGAA AAAGCCATTT CAGAACCCAA TTTCTCTGTT
    GCATATGCCA ACATGTGTCG TTGCCTTATG GGGCTTAAAG TTCCAACCAC AGACAAGCCA
    GGGGTTACAG TCAACTTCCG CAAGTTGTTA CTGAATCGGT GCCAGAAGGA GTTTGAGAAG
    GATAAGGATG ATGATGAAGT GTTTGAGAAG AAACAAAAAG AAATGGATGC TGCTGCTTCG
    CCGGAGGAAA AAGCACGTTT GAATGATGAA CTGGTCGAGG CCCGTGACAG TGCACGACGG
    CGATCCTTGG GGAACATTAA GTTCATTGGA GAACTGTTTA AGTTAAAAAT GCTGACTGAA
    GCTATAATGC ACGATTGTGT GGTGAAGCTA TTAAAAAATC ATGATGAGGA ATCTCTGGAG
    TGCCTCTGCC GACTGCTGTC CACCATTGGC AAGGACCTGG ATTTTGAGAG AGCTAAGCCA
    CGTATGGACC AGTACTTCAA CCAGATGGAT AAAATCATTA AGGAGCGGAA GACATCTTCA
    CGAATTAGAT TCATGCTGCA GGATGTGATT GATTTGCGTC TGTGTAATTG GGTGCCTCGT
    CGTGCAGATC AGGGCCCTAA AACTATAGAT CAGATTCATA AAGAAGCAGA AATGGAAAAG
    CAGAGGGAGC AGGTTAAGGT GCAACAACTG ATGTCTAAAC AAGACAAGCG GCGAGAGCCC
    CCAGGACGAC CCACTGGAAG CCGGGGTAAC CAGCCTGCAG ATGATGGCTG GAATACAGTG
    CCCATCAGTA AGGGCAGCCG TCCTATTGAC ACCACTAGGA TCACAAAGAT AACAAAGCTT
    GGTGCTATTG ATTCAAATCA ACTGCTTGCC CCAGGAGGAC GTCTGAGTTG GGGTAAAGGA
    AGCAGCGGAG GAACTGGAGC CAAACCAGCA GAAACAGCTC CAGAATCTAG TCGGCCTGCG
    ACCAGCACCT TAAACAGGTT TTCTGCCTTG CAGCAGTCTT CATCTGCTGA GAGTCAAGAT
    GCTAGACGGC CCTTGCATCG GGGAAGTTCT AGTCGGGAAC GTTCTGAAAA AGATCGTGGA
    GACAGATTTG ATAGAGGTGA AAGGTTTGAT AAGGGAGAAC TGTTTGATAG ATCTGACCGT
    TCAGATCGAG CTCGTCCTCT TCTTACAAAG AGAAGCTATA GCAAGGAAAC TGAGGACCGT
    AGCCGAGAAA GAGAGGCCCA TATACCACAG GAGGCTGTGA GGAAGGTGGC AAGTATGACT
    GAGCAGCGGG ACCGCAGCAA AGAAAGTGTG AAAAAAGAAA TTCCAGCACC GACAGCAGTG
    GTTGCCTCAT CAAAGCCCTC TATGTCTGAG GATGAGCTAG AGAAGAAATC TAAAGCCATC
    ATAGAAGAAT ATTTGCACAT TAATGATATG AAGGAAGCTC TTCAGTGTGT AGTGGAGCTT
    AATAGTTCAA CGCTGCTTTT TATCTTTGTG CGGAATGGGA TTGAGTCAAC ACTAGAGAGA
    AGCACTATTG CCAGAGAGCA TATGGGTCTC CTTTTGTACC AGTTAGTAAA GGCAGGAACT
    CTGTCCAAGG AGCAGTATTA CAAAGGGGTG CTTGAAGTTC TTGAAGTAGC AGAAGATATG
    GAAATAGATA TACCCCACAT CTGGCTGTAT TTAGCAGAGA TAATCAGCCC TGTGCTGCTA
    GAGGGGGGGA TTCCGATGGG GGAGCTGTTC AGAGAGCTGA CCAAACCTTT AGTGCTCATT
    GGCAAAGCTG GAGTCCTTCT GGCAGAAATC CTTGGATTAT TGTGCAAAGG CATGAGTCAT
    AAGAAGGCTG GAGCCCTGTG GAAAGAATCT GGGCTAAGCT GGAAGGAATT CTTGTCCAAG
    GACCAGGATA TTAACAAGTT TATAACTGAC AAGAAACTCG AATACACAAC AGGAGAGGAG
    ACTGAAACAC CCAGTAAGAA AGACCTCACC CCTGCTGAAC TTGATAAAAT TTTGACAAAA
    CTTTTGAAAG AGAACAGCAG TAACCAGAAG ATATTTGACT GGATTGAAGC CAACTTAAAT
    GACCAGCAAA CTTCATCCAA CACCTTTGTT CGAACTTTAA TGAGTACTGT GTGCCACTCT
    GCTATTATTT TTGAGAATCC TTGTAGGGTT GATGTTAATA TTATCCATAC TAGAGCCAAA
    TTTCTTCAGA AATATTTAAG TGACGAGGCC AAGGAACTAC AGGCTCTCTA TGCTCTACAG
    GCACTTGTTG TAAAGCTTGA CCAGCCCCCA AATCTCCTAC GGATGTTTTT TGATGCTCTC
    TATGATGAGG ATGTGATAAA AGAGGATCCA TTCTACAAGT GGGAGTCCAG CAAAGACCCA
    GCTGAACAGC AAGGAAAAGG TGTTGCTCTT AAATCTGTCA CTGCCTTCTT CACATGGCTA
    CGGGAAGCAG AGGATGAGTC TGATAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004914455.1
    CDS137..4966
    Translation

    Target ORF information:

    RefSeq Version XM_004914398.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004914398.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
    CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA
    CAAATGAATA CACAATCTCA GCCTCGACAG GCAGGATTCA GATCAATCCA GCATTATTAT
    CAAAATAGGA CACAGCCTCC CACTAGTGCC ACCAGAGTGC AGAGTGGTTC TTCTGCTCGA
    GCAGTTACTC CAGCCCATGT GTATCAACCA GGCTCCCAAG TTATGATGAT TCCTTCACAG
    ATTTCATACC CTTCCCAGGG ATACTACATT CAGGGACAGG GACGCTCATA TGTGGTGCCC
    ACAAACCAGT ACCCTGTGCA GACTGGGGCT CCAGGTTTCT TCCCCAGTGC AAGTCCCTCA
    GAATATGGCA CATATGCTGG AGCATATTAC CCTGCACAAC AGACGTTCCC TCCTGGAGTG
    CCTGGAGCAC AAGTTATGAT GAATCAGCCA CAGGTGATCC CTCCTAAACG GGAGCGCAAA
    ACGATAAGAA TTAGAGACCC CAACCAAGGA GGCAAAGATA TCACAGAAGA AATTCTCTCA
    GGAGGAAGAA CATCCTCTAC GCCAACGCCT CCACAGTCAA CTGTGGGAAG TTCTGAAATT
    CAAGCTAATG GAGAAAGCGT TCATCATGTT ACCGTTGTTG TGCGCCCAGA TGATGGACCC
    AAGGCCACCC AAGTGCTGAT CAAAGCCTCA GAGAGCCCTT CCCCTCCTCT GCCTCTCTCT
    GAAGCTGAGC CAAGCATAGC AAATGTGTGT GATATTTCAA AAGATAACAC TATCTCTGAG
    TTGACTTCTC ATATAGTAGA GCCCCTAAGT TCAGAAGAGA CAACCATTGA TACTTCTACC
    TCTCAGGTGG ATCTCAAACA CCAGCCAGTG GCTGCTATGG CAGAGCATGA AACTCTTATA
    GAGGAGGCAT CAATACAGGA GGTGGCCTTT GCAGAGGCAC CCATCGCTCA ATCAGAGATA
    CCAAAATCTC TTGCCTCCCA GCCTCTAGCA GAAATCTCTA CCCCTGAACA GATTTCTAAA
    CTTGACGCAC AAGTGCCATC TTTGCAACAA GAGGAGTTAC TTACAGAAAC TGTTGAACTT
    AATTTAAGCA ATCAGGCTCT TCCTTCACAA ATTTCTGAGC CTGAACCTTT TGAACCTGAG
    GCTTCAGCTT CAGATCCTCT ACATGAGGAG CCCACAGAGC CCACAGTTTC AACAGAAGAA
    CCACAAGCTA ATGGACTGTC TGAGGAGACA CCAGTATTAC CTGTACAGCC ACAACCAACA
    TCTGTTTGCC AGACTGAGCC CTCCCTAGAA TCCCCCATCG CTCAGCCTGA GGAGCTGCTT
    CTAACAAATG GAGGTGGACC ACCTGGCCTA CAAGAAAATG ATGCACCTGA TTCACAGGTT
    GAAGCTCATG TTAGCCCAAT CACTGAGCCT GAGGACCAAC CTGTGACAGT CTCTGATAAC
    GTGCTTGTGG AAGAGGATGT AGAAAAAACA TTGCCTGCGG ATACGGAAGT CGTGGCTTCA
    ACCCAAGTTG TAAAGCAGAG CTCCGAGAGT CAGTCTCAGG TTGCTGTTGC TGCACCCAAG
    AAAAAGCGAA AAATGAAGGA ACTAAACAAG AAAGAGGCAG TGGGAGATCT CCTTGATGCG
    TTCAAAGAGG CTGGTGATGG CTCTTCTGAT GTGGATTCTT CCTCTAAGCC TACCAGTGCT
    GCACCTGAAC CAGATCAGAG CCCTTGTGCT CCTCCTGAGG AACAGGATGA AACTTGGGAA
    GAGAAGGAAG ATAAACTGGA TGCAGAGAAT ATTAAACCAG GAGAACATAA ATATAAATAC
    AAAGAAGAGC AATGGAAACC CCTAAACCCG GAGGAAAAGA AGAGTTATGA TCGCGAGTTT
    CTGCTAGGGT TCCAGTTTAT TGTGGCCAGT ATGCAGAAAC CTGCGGGTCT TCCTCAAATT
    ACTGATGTGG TGCTGGATAA GGCAAACAAA ACTCCATTGC GACCATTGGA TCCTACACGT
    CTGACGGGAA TGAACTGTGG GCCAGATTTT ACACCTTCAT TTGCCAACCT TGGTCGGCCT
    GCTGTGGGTA ACCGTGGACC ACCAGCTGGC ATGGGTCCGA GACGGTCCCA GCAGGGTCCA
    CGAAAAGAGC CTCGTAAAAT TATTCAAACC GTATCATTAA ATGAGAATGT ACAACTAAAC
    AAAGCTGAAA AGGCATGGAA ACCGATAAAC AAGAGAGCTG GCGATGAGGA GGACCCAGAA
    AATATTAAGA CACAGGACCT ATTCCGAAGG GTTCGCAGTA TCTTGAATAA GCTGACCCCT
    CAGATGTTCC AGCAGCTGAT GAAGCAGGTC ACTGACCTTA CTATTGATAC CGAAGATCGT
    CTTAAGGGTG TCATTGACCT AATTTTTGAA AAAGCCATTT CAGAACCCAA TTTCTCTGTT
    GCATATGCCA ACATGTGTCG TTGCCTTATG GGGCTTAAAG TTCCAACCAC AGACAAGCCA
    GGGGTTACAG TCAACTTCCG CAAGTTGTTA CTGAATCGGT GCCAGAAGGA GTTTGAGAAG
    GATAAGGATG ATGATGAAGT GTTTGAGAAG AAACAAAAAG AAATGGATGC TGCTGCTTCG
    CCGGAGGAAA AAGCACGTTT GAATGATGAA CTGGTCGAGG CCCGTGACAG TGCACGACGG
    CGATCCTTGG GGAACATTAA GTTCATTGGA GAACTGTTTA AGTTAAAAAT GCTGACTGAA
    GCTATAATGC ACGATTGTGT GGTGAAGCTA TTAAAAAATC ATGATGAGGA ATCTCTGGAG
    TGCCTCTGCC GACTGCTGTC CACCATTGGC AAGGACCTGG ATTTTGAGAG AGCTAAGCCA
    CGTATGGACC AGTACTTCAA CCAGATGGAT AAAATCATTA AGGAGCGGAA GACATCTTCA
    CGAATTAGAT TCATGCTGCA GGATGTGATT GATTTGCGTC TGTGTAATTG GGTGCCTCGT
    CGTGCAGATC AGGGCCCTAA AACTATAGAT CAGATTCATA AAGAAGCAGA AATGGAAAAG
    CAGAGGGAGC AGGTTAAGGT GCAACAACTG ATGTCTAAAC AAGACAAGCG GCGAGAGCCC
    CCAGGACGAC CCACTGGAAG CCGGGGTAAC CAGCCTGCAG ATGATGGCTG GAATACAGTG
    CCCATCAGTA AGGGCAGCCG TCCTATTGAC ACCACTAGGA TCACAAAGAT AACAAAGCTT
    GGTGCTATTG ATTCAAATCA ACTGCTTGCC CCAGGAGGAC GTCTGAGTTG GGGTAAAGGA
    AGCAGCGGAG GAACTGGAGC CAAACCAGCA GAAACAGCTC CAGAATCTAG TCGGCCTGCG
    ACCAGCACCT TAAACAGGTT TTCTGCCTTG CAGCAGTCTT CATCTGCTGA GAGTCAAGAT
    GCTAGACGGC CCTTGCATCG GGGAAGTTCT AGTCGGGAAC GTTCTGAAAA AGATCGTGGA
    GACAGATTTG ATAGAGGTGA AAGGTTTGAT AAGGGAGAAC TGTTTGATAG ATCTGACCGT
    TCAGATCGAG CTCGTCCTCT TCTTACAAAG AGAAGCTATA GCAAGGAAAC TGAGGACCGT
    AGCCGAGAAA GAGAGGCCCA TATACCACAG GAGGCTGTGA GGAAGGTGGC AAGTATGACT
    GAGCAGCGGG ACCGCAGCAA AGAAAGTGTG AAAAAAGAAA TTCCAGCACC GACAGCAGTG
    GTTGCCTCAT CAAAGCCCTC TATGTCTGAG GATGAGCTAG AGAAGAAATC TAAAGCCATC
    ATAGAAGAAT ATTTGCACAT TAATGATATG AAGGAAGCTC TTCAGTGTGT AGTGGAGCTT
    AATAGTTCAA CGCTGCTTTT TATCTTTGTG CGGAATGGGA TTGAGTCAAC ACTAGAGAGA
    AGCACTATTG CCAGAGAGCA TATGGGTCTC CTTTTGTACC AGTTAGTAAA GGCAGGAACT
    CTGTCCAAGG AGCAGTATTA CAAAGGGGTG CTTGAAGTTC TTGAAGTAGC AGAAGATATG
    GAAATAGATA TACCCCACAT CTGGCTGTAT TTAGCAGAGA TAATCAGCCC TGTGCTGCTA
    GAGGGGGGGA TTCCGATGGG GGAGCTGTTC AGAGAGCTGA CCAAACCTTT AGTGCTCATT
    GGCAAAGCTG GAGTCCTTCT GGCAGAAATC CTTGGATTAT TGTGCAAAGG CATGAGTCAT
    AAGAAGGCTG GAGCCCTGTG GAAAGAATCT GGGCTAAGCT GGAAGGAATT CTTGTCCAAG
    GACCAGGATA TTAACAAGTT TATAACTGAC AAGAAACTCG AATACACAAC AGGAGAGGAG
    ACTGAAACAC CCAGTAAGAA AGACCTCACC CCTGCTGAAC TTGATAAAAT TTTGACAAAA
    CTTTTGAAAG AGAACAGCAG TAACCAGAAG ATATTTGACT GGATTGAAGC CAACTTAAAT
    GACCAGCAAA CTTCATCCAA CACCTTTGTT CGAACTTTAA TGAGTACTGT GTGCCACTCT
    GCTATTATTT TTGAGAATCC TTGTAGGGTT GATGTTAATA TTATCCATAC TAGAGCCAAA
    TTTCTTCAGA AATATTTAAG TGACGAGGCC AAGGAACTAC AGGCTCTCTA TGCTCTACAG
    GCACTTGTTG TAAAGCTTGA CCAGCCCCCA AATCTCCTAC GGATGTTTTT TGATGCTCTC
    TATGATGAGG ATGTGATAAA AGAGGATCCA TTCTACAAGT GGGAGTCCAG CAAAGACCCA
    GCTGAACAGC AAGGAAAAGG TGTTGCTCTT AAATCTGTCA CTGCCTTCTT CACATGGCTA
    CGGGAAGCAG AGGATGAGTC TGATAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa10204
    Clone ID Related Accession (Same CDS sequence) XM_018094420.1 , XM_004914398.4 , XM_004914398.3 , XM_002935144.5 , XM_018094419.1 , XM_012963377.3 , XM_002935144.4 , XM_012963377.2
    Accession Version XM_002935144.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4830bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002935144.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
    CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA
    CAAATGAATA CACAATCTCA GCCTCGACAG GCAGGATTCA GATCAATCCA GCATTATTAT
    CAAAATAGGA CACAGCCTCC CACTAGTGCC ACCAGAGTGC AGAGTGGTTC TTCTGCTCGA
    GCAGTTACTC CAGCCCATGT GTATCAACCA GGCTCCCAAG TTATGATGAT TCCTTCACAG
    ATTTCATACC CTTCCCAGGG ATACTACATT CAGGGACAGG GACGCTCATA TGTGGTGCCC
    ACAAACCAGT ACCCTGTGCA GACTGGGGCT CCAGGTTTCT TCCCCAGTGC AAGTCCCTCA
    GAATATGGCA CATATGCTGG AGCATATTAC CCTGCACAAC AGACGTTCCC TCCTGGAGTG
    CCTGGAGCAC AAGTTATGAT GAATCAGCCA CAGGTGATCC CTCCTAAACG GGAGCGCAAA
    ACGATAAGAA TTAGAGACCC CAACCAAGGA GGCAAAGATA TCACAGAAGA AATTCTCTCA
    GGAGGAAGAA CATCCTCTAC GCCAACGCCT CCACAGTCAA CTGTGGGAAG TTCTGAAATT
    CAAGCTAATG GAGAAAGCGT TCATCATGTT ACCGTTGTTG TGCGCCCAGA TGATGGACCC
    AAGGCCACCC AAGTGCTGAT CAAAGCCTCA GAGAGCCCTT CCCCTCCTCT GCCTCTCTCT
    GAAGCTGAGC CAAGCATAGC AAATGTGTGT GATATTTCAA AAGATAACAC TATCTCTGAG
    TTGACTTCTC ATATAGTAGA GCCCCTAAGT TCAGAAGAGA CAACCATTGA TACTTCTACC
    TCTCAGGTGG ATCTCAAACA CCAGCCAGTG GCTGCTATGG CAGAGCATGA AACTCTTATA
    GAGGAGGCAT CAATACAGGA GGTGGCCTTT GCAGAGGCAC CCATCGCTCA ATCAGAGATA
    CCAAAATCTC TTGCCTCCCA GCCTCTAGCA GAAATCTCTA CCCCTGAACA GATTTCTAAA
    CTTGACGCAC AAGTGCCATC TTTGCAACAA GAGGAGTTAC TTACAGAAAC TGTTGAACTT
    AATTTAAGCA ATCAGGCTCT TCCTTCACAA ATTTCTGAGC CTGAACCTTT TGAACCTGAG
    GCTTCAGCTT CAGATCCTCT ACATGAGGAG CCCACAGAGC CCACAGTTTC AACAGAAGAA
    CCACAAGCTA ATGGACTGTC TGAGGAGACA CCAGTATTAC CTGTACAGCC ACAACCAACA
    TCTGTTTGCC AGACTGAGCC CTCCCTAGAA TCCCCCATCG CTCAGCCTGA GGAGCTGCTT
    CTAACAAATG GAGGTGGACC ACCTGGCCTA CAAGAAAATG ATGCACCTGA TTCACAGGTT
    GAAGCTCATG TTAGCCCAAT CACTGAGCCT GAGGACCAAC CTGTGACAGT CTCTGATAAC
    GTGCTTGTGG AAGAGGATGT AGAAAAAACA TTGCCTGCGG ATACGGAAGT CGTGGCTTCA
    ACCCAAGTTG TAAAGCAGAG CTCCGAGAGT CAGTCTCAGG TTGCTGTTGC TGCACCCAAG
    AAAAAGCGAA AAATGAAGGA ACTAAACAAG AAAGAGGCAG TGGGAGATCT CCTTGATGCG
    TTCAAAGAGG CTGGTGATGG CTCTTCTGAT GTGGATTCTT CCTCTAAGCC TACCAGTGCT
    GCACCTGAAC CAGATCAGAG CCCTTGTGCT CCTCCTGAGG AACAGGATGA AACTTGGGAA
    GAGAAGGAAG ATAAACTGGA TGCAGAGAAT ATTAAACCAG GAGAACATAA ATATAAATAC
    AAAGAAGAGC AATGGAAACC CCTAAACCCG GAGGAAAAGA AGAGTTATGA TCGCGAGTTT
    CTGCTAGGGT TCCAGTTTAT TGTGGCCAGT ATGCAGAAAC CTGCGGGTCT TCCTCAAATT
    ACTGATGTGG TGCTGGATAA GGCAAACAAA ACTCCATTGC GACCATTGGA TCCTACACGT
    CTGACGGGAA TGAACTGTGG GCCAGATTTT ACACCTTCAT TTGCCAACCT TGGTCGGCCT
    GCTGTGGGTA ACCGTGGACC ACCAGCTGGC ATGGGTCCGA GACGGTCCCA GCAGGGTCCA
    CGAAAAGAGC CTCGTAAAAT TATTCAAACC GTATCATTAA ATGAGAATGT ACAACTAAAC
    AAAGCTGAAA AGGCATGGAA ACCGATAAAC AAGAGAGCTG GCGATGAGGA GGACCCAGAA
    AATATTAAGA CACAGGACCT ATTCCGAAGG GTTCGCAGTA TCTTGAATAA GCTGACCCCT
    CAGATGTTCC AGCAGCTGAT GAAGCAGGTC ACTGACCTTA CTATTGATAC CGAAGATCGT
    CTTAAGGGTG TCATTGACCT AATTTTTGAA AAAGCCATTT CAGAACCCAA TTTCTCTGTT
    GCATATGCCA ACATGTGTCG TTGCCTTATG GGGCTTAAAG TTCCAACCAC AGACAAGCCA
    GGGGTTACAG TCAACTTCCG CAAGTTGTTA CTGAATCGGT GCCAGAAGGA GTTTGAGAAG
    GATAAGGATG ATGATGAAGT GTTTGAGAAG AAACAAAAAG AAATGGATGC TGCTGCTTCG
    CCGGAGGAAA AAGCACGTTT GAATGATGAA CTGGTCGAGG CCCGTGACAG TGCACGACGG
    CGATCCTTGG GGAACATTAA GTTCATTGGA GAACTGTTTA AGTTAAAAAT GCTGACTGAA
    GCTATAATGC ACGATTGTGT GGTGAAGCTA TTAAAAAATC ATGATGAGGA ATCTCTGGAG
    TGCCTCTGCC GACTGCTGTC CACCATTGGC AAGGACCTGG ATTTTGAGAG AGCTAAGCCA
    CGTATGGACC AGTACTTCAA CCAGATGGAT AAAATCATTA AGGAGCGGAA GACATCTTCA
    CGAATTAGAT TCATGCTGCA GGATGTGATT GATTTGCGTC TGTGTAATTG GGTGCCTCGT
    CGTGCAGATC AGGGCCCTAA AACTATAGAT CAGATTCATA AAGAAGCAGA AATGGAAAAG
    CAGAGGGAGC AGGTTAAGGT GCAACAACTG ATGTCTAAAC AAGACAAGCG GCGAGAGCCC
    CCAGGACGAC CCACTGGAAG CCGGGGTAAC CAGCCTGCAG ATGATGGCTG GAATACAGTG
    CCCATCAGTA AGGGCAGCCG TCCTATTGAC ACCACTAGGA TCACAAAGAT AACAAAGCTT
    GGTGCTATTG ATTCAAATCA ACTGCTTGCC CCAGGAGGAC GTCTGAGTTG GGGTAAAGGA
    AGCAGCGGAG GAACTGGAGC CAAACCAGCA GAAACAGCTC CAGAATCTAG TCGGCCTGCG
    ACCAGCACCT TAAACAGGTT TTCTGCCTTG CAGCAGTCTT CATCTGCTGA GAGTCAAGAT
    GCTAGACGGC CCTTGCATCG GGGAAGTTCT AGTCGGGAAC GTTCTGAAAA AGATCGTGGA
    GACAGATTTG ATAGAGGTGA AAGGTTTGAT AAGGGAGAAC TGTTTGATAG ATCTGACCGT
    TCAGATCGAG CTCGTCCTCT TCTTACAAAG AGAAGCTATA GCAAGGAAAC TGAGGACCGT
    AGCCGAGAAA GAGAGGCCCA TATACCACAG GAGGCTGTGA GGAAGGTGGC AAGTATGACT
    GAGCAGCGGG ACCGCAGCAA AGAAAGTGTG AAAAAAGAAA TTCCAGCACC GACAGCAGTG
    GTTGCCTCAT CAAAGCCCTC TATGTCTGAG GATGAGCTAG AGAAGAAATC TAAAGCCATC
    ATAGAAGAAT ATTTGCACAT TAATGATATG AAGGAAGCTC TTCAGTGTGT AGTGGAGCTT
    AATAGTTCAA CGCTGCTTTT TATCTTTGTG CGGAATGGGA TTGAGTCAAC ACTAGAGAGA
    AGCACTATTG CCAGAGAGCA TATGGGTCTC CTTTTGTACC AGTTAGTAAA GGCAGGAACT
    CTGTCCAAGG AGCAGTATTA CAAAGGGGTG CTTGAAGTTC TTGAAGTAGC AGAAGATATG
    GAAATAGATA TACCCCACAT CTGGCTGTAT TTAGCAGAGA TAATCAGCCC TGTGCTGCTA
    GAGGGGGGGA TTCCGATGGG GGAGCTGTTC AGAGAGCTGA CCAAACCTTT AGTGCTCATT
    GGCAAAGCTG GAGTCCTTCT GGCAGAAATC CTTGGATTAT TGTGCAAAGG CATGAGTCAT
    AAGAAGGCTG GAGCCCTGTG GAAAGAATCT GGGCTAAGCT GGAAGGAATT CTTGTCCAAG
    GACCAGGATA TTAACAAGTT TATAACTGAC AAGAAACTCG AATACACAAC AGGAGAGGAG
    ACTGAAACAC CCAGTAAGAA AGACCTCACC CCTGCTGAAC TTGATAAAAT TTTGACAAAA
    CTTTTGAAAG AGAACAGCAG TAACCAGAAG ATATTTGACT GGATTGAAGC CAACTTAAAT
    GACCAGCAAA CTTCATCCAA CACCTTTGTT CGAACTTTAA TGAGTACTGT GTGCCACTCT
    GCTATTATTT TTGAGAATCC TTGTAGGGTT GATGTTAATA TTATCCATAC TAGAGCCAAA
    TTTCTTCAGA AATATTTAAG TGACGAGGCC AAGGAACTAC AGGCTCTCTA TGCTCTACAG
    GCACTTGTTG TAAAGCTTGA CCAGCCCCCA AATCTCCTAC GGATGTTTTT TGATGCTCTC
    TATGATGAGG ATGTGATAAA AGAGGATCCA TTCTACAAGT GGGAGTCCAG CAAAGACCCA
    GCTGAACAGC AAGGAAAAGG TGTTGCTCTT AAATCTGTCA CTGCCTTCTT CACATGGCTA
    CGGGAAGCAG AGGATGAGTC TGATAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002935190.1
    CDS214..5043
    Translation

    Target ORF information:

    RefSeq Version XM_002935144.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002935144.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
    CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA
    CAAATGAATA CACAATCTCA GCCTCGACAG GCAGGATTCA GATCAATCCA GCATTATTAT
    CAAAATAGGA CACAGCCTCC CACTAGTGCC ACCAGAGTGC AGAGTGGTTC TTCTGCTCGA
    GCAGTTACTC CAGCCCATGT GTATCAACCA GGCTCCCAAG TTATGATGAT TCCTTCACAG
    ATTTCATACC CTTCCCAGGG ATACTACATT CAGGGACAGG GACGCTCATA TGTGGTGCCC
    ACAAACCAGT ACCCTGTGCA GACTGGGGCT CCAGGTTTCT TCCCCAGTGC AAGTCCCTCA
    GAATATGGCA CATATGCTGG AGCATATTAC CCTGCACAAC AGACGTTCCC TCCTGGAGTG
    CCTGGAGCAC AAGTTATGAT GAATCAGCCA CAGGTGATCC CTCCTAAACG GGAGCGCAAA
    ACGATAAGAA TTAGAGACCC CAACCAAGGA GGCAAAGATA TCACAGAAGA AATTCTCTCA
    GGAGGAAGAA CATCCTCTAC GCCAACGCCT CCACAGTCAA CTGTGGGAAG TTCTGAAATT
    CAAGCTAATG GAGAAAGCGT TCATCATGTT ACCGTTGTTG TGCGCCCAGA TGATGGACCC
    AAGGCCACCC AAGTGCTGAT CAAAGCCTCA GAGAGCCCTT CCCCTCCTCT GCCTCTCTCT
    GAAGCTGAGC CAAGCATAGC AAATGTGTGT GATATTTCAA AAGATAACAC TATCTCTGAG
    TTGACTTCTC ATATAGTAGA GCCCCTAAGT TCAGAAGAGA CAACCATTGA TACTTCTACC
    TCTCAGGTGG ATCTCAAACA CCAGCCAGTG GCTGCTATGG CAGAGCATGA AACTCTTATA
    GAGGAGGCAT CAATACAGGA GGTGGCCTTT GCAGAGGCAC CCATCGCTCA ATCAGAGATA
    CCAAAATCTC TTGCCTCCCA GCCTCTAGCA GAAATCTCTA CCCCTGAACA GATTTCTAAA
    CTTGACGCAC AAGTGCCATC TTTGCAACAA GAGGAGTTAC TTACAGAAAC TGTTGAACTT
    AATTTAAGCA ATCAGGCTCT TCCTTCACAA ATTTCTGAGC CTGAACCTTT TGAACCTGAG
    GCTTCAGCTT CAGATCCTCT ACATGAGGAG CCCACAGAGC CCACAGTTTC AACAGAAGAA
    CCACAAGCTA ATGGACTGTC TGAGGAGACA CCAGTATTAC CTGTACAGCC ACAACCAACA
    TCTGTTTGCC AGACTGAGCC CTCCCTAGAA TCCCCCATCG CTCAGCCTGA GGAGCTGCTT
    CTAACAAATG GAGGTGGACC ACCTGGCCTA CAAGAAAATG ATGCACCTGA TTCACAGGTT
    GAAGCTCATG TTAGCCCAAT CACTGAGCCT GAGGACCAAC CTGTGACAGT CTCTGATAAC
    GTGCTTGTGG AAGAGGATGT AGAAAAAACA TTGCCTGCGG ATACGGAAGT CGTGGCTTCA
    ACCCAAGTTG TAAAGCAGAG CTCCGAGAGT CAGTCTCAGG TTGCTGTTGC TGCACCCAAG
    AAAAAGCGAA AAATGAAGGA ACTAAACAAG AAAGAGGCAG TGGGAGATCT CCTTGATGCG
    TTCAAAGAGG CTGGTGATGG CTCTTCTGAT GTGGATTCTT CCTCTAAGCC TACCAGTGCT
    GCACCTGAAC CAGATCAGAG CCCTTGTGCT CCTCCTGAGG AACAGGATGA AACTTGGGAA
    GAGAAGGAAG ATAAACTGGA TGCAGAGAAT ATTAAACCAG GAGAACATAA ATATAAATAC
    AAAGAAGAGC AATGGAAACC CCTAAACCCG GAGGAAAAGA AGAGTTATGA TCGCGAGTTT
    CTGCTAGGGT TCCAGTTTAT TGTGGCCAGT ATGCAGAAAC CTGCGGGTCT TCCTCAAATT
    ACTGATGTGG TGCTGGATAA GGCAAACAAA ACTCCATTGC GACCATTGGA TCCTACACGT
    CTGACGGGAA TGAACTGTGG GCCAGATTTT ACACCTTCAT TTGCCAACCT TGGTCGGCCT
    GCTGTGGGTA ACCGTGGACC ACCAGCTGGC ATGGGTCCGA GACGGTCCCA GCAGGGTCCA
    CGAAAAGAGC CTCGTAAAAT TATTCAAACC GTATCATTAA ATGAGAATGT ACAACTAAAC
    AAAGCTGAAA AGGCATGGAA ACCGATAAAC AAGAGAGCTG GCGATGAGGA GGACCCAGAA
    AATATTAAGA CACAGGACCT ATTCCGAAGG GTTCGCAGTA TCTTGAATAA GCTGACCCCT
    CAGATGTTCC AGCAGCTGAT GAAGCAGGTC ACTGACCTTA CTATTGATAC CGAAGATCGT
    CTTAAGGGTG TCATTGACCT AATTTTTGAA AAAGCCATTT CAGAACCCAA TTTCTCTGTT
    GCATATGCCA ACATGTGTCG TTGCCTTATG GGGCTTAAAG TTCCAACCAC AGACAAGCCA
    GGGGTTACAG TCAACTTCCG CAAGTTGTTA CTGAATCGGT GCCAGAAGGA GTTTGAGAAG
    GATAAGGATG ATGATGAAGT GTTTGAGAAG AAACAAAAAG AAATGGATGC TGCTGCTTCG
    CCGGAGGAAA AAGCACGTTT GAATGATGAA CTGGTCGAGG CCCGTGACAG TGCACGACGG
    CGATCCTTGG GGAACATTAA GTTCATTGGA GAACTGTTTA AGTTAAAAAT GCTGACTGAA
    GCTATAATGC ACGATTGTGT GGTGAAGCTA TTAAAAAATC ATGATGAGGA ATCTCTGGAG
    TGCCTCTGCC GACTGCTGTC CACCATTGGC AAGGACCTGG ATTTTGAGAG AGCTAAGCCA
    CGTATGGACC AGTACTTCAA CCAGATGGAT AAAATCATTA AGGAGCGGAA GACATCTTCA
    CGAATTAGAT TCATGCTGCA GGATGTGATT GATTTGCGTC TGTGTAATTG GGTGCCTCGT
    CGTGCAGATC AGGGCCCTAA AACTATAGAT CAGATTCATA AAGAAGCAGA AATGGAAAAG
    CAGAGGGAGC AGGTTAAGGT GCAACAACTG ATGTCTAAAC AAGACAAGCG GCGAGAGCCC
    CCAGGACGAC CCACTGGAAG CCGGGGTAAC CAGCCTGCAG ATGATGGCTG GAATACAGTG
    CCCATCAGTA AGGGCAGCCG TCCTATTGAC ACCACTAGGA TCACAAAGAT AACAAAGCTT
    GGTGCTATTG ATTCAAATCA ACTGCTTGCC CCAGGAGGAC GTCTGAGTTG GGGTAAAGGA
    AGCAGCGGAG GAACTGGAGC CAAACCAGCA GAAACAGCTC CAGAATCTAG TCGGCCTGCG
    ACCAGCACCT TAAACAGGTT TTCTGCCTTG CAGCAGTCTT CATCTGCTGA GAGTCAAGAT
    GCTAGACGGC CCTTGCATCG GGGAAGTTCT AGTCGGGAAC GTTCTGAAAA AGATCGTGGA
    GACAGATTTG ATAGAGGTGA AAGGTTTGAT AAGGGAGAAC TGTTTGATAG ATCTGACCGT
    TCAGATCGAG CTCGTCCTCT TCTTACAAAG AGAAGCTATA GCAAGGAAAC TGAGGACCGT
    AGCCGAGAAA GAGAGGCCCA TATACCACAG GAGGCTGTGA GGAAGGTGGC AAGTATGACT
    GAGCAGCGGG ACCGCAGCAA AGAAAGTGTG AAAAAAGAAA TTCCAGCACC GACAGCAGTG
    GTTGCCTCAT CAAAGCCCTC TATGTCTGAG GATGAGCTAG AGAAGAAATC TAAAGCCATC
    ATAGAAGAAT ATTTGCACAT TAATGATATG AAGGAAGCTC TTCAGTGTGT AGTGGAGCTT
    AATAGTTCAA CGCTGCTTTT TATCTTTGTG CGGAATGGGA TTGAGTCAAC ACTAGAGAGA
    AGCACTATTG CCAGAGAGCA TATGGGTCTC CTTTTGTACC AGTTAGTAAA GGCAGGAACT
    CTGTCCAAGG AGCAGTATTA CAAAGGGGTG CTTGAAGTTC TTGAAGTAGC AGAAGATATG
    GAAATAGATA TACCCCACAT CTGGCTGTAT TTAGCAGAGA TAATCAGCCC TGTGCTGCTA
    GAGGGGGGGA TTCCGATGGG GGAGCTGTTC AGAGAGCTGA CCAAACCTTT AGTGCTCATT
    GGCAAAGCTG GAGTCCTTCT GGCAGAAATC CTTGGATTAT TGTGCAAAGG CATGAGTCAT
    AAGAAGGCTG GAGCCCTGTG GAAAGAATCT GGGCTAAGCT GGAAGGAATT CTTGTCCAAG
    GACCAGGATA TTAACAAGTT TATAACTGAC AAGAAACTCG AATACACAAC AGGAGAGGAG
    ACTGAAACAC CCAGTAAGAA AGACCTCACC CCTGCTGAAC TTGATAAAAT TTTGACAAAA
    CTTTTGAAAG AGAACAGCAG TAACCAGAAG ATATTTGACT GGATTGAAGC CAACTTAAAT
    GACCAGCAAA CTTCATCCAA CACCTTTGTT CGAACTTTAA TGAGTACTGT GTGCCACTCT
    GCTATTATTT TTGAGAATCC TTGTAGGGTT GATGTTAATA TTATCCATAC TAGAGCCAAA
    TTTCTTCAGA AATATTTAAG TGACGAGGCC AAGGAACTAC AGGCTCTCTA TGCTCTACAG
    GCACTTGTTG TAAAGCTTGA CCAGCCCCCA AATCTCCTAC GGATGTTTTT TGATGCTCTC
    TATGATGAGG ATGTGATAAA AGAGGATCCA TTCTACAAGT GGGAGTCCAG CAAAGACCCA
    GCTGAACAGC AAGGAAAAGG TGTTGCTCTT AAATCTGTCA CTGCCTTCTT CACATGGCTA
    CGGGAAGCAG AGGATGAGTC TGATAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa10204
    Clone ID Related Accession (Same CDS sequence) XM_018094420.1 , XM_004914398.4 , XM_004914398.3 , XM_002935144.5 , XM_018094419.1 , XM_012963377.3 , XM_002935144.4 , XM_012963377.2
    Accession Version XM_012963377.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4830bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012963377.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
    CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA
    CAAATGAATA CACAATCTCA GCCTCGACAG GCAGGATTCA GATCAATCCA GCATTATTAT
    CAAAATAGGA CACAGCCTCC CACTAGTGCC ACCAGAGTGC AGAGTGGTTC TTCTGCTCGA
    GCAGTTACTC CAGCCCATGT GTATCAACCA GGCTCCCAAG TTATGATGAT TCCTTCACAG
    ATTTCATACC CTTCCCAGGG ATACTACATT CAGGGACAGG GACGCTCATA TGTGGTGCCC
    ACAAACCAGT ACCCTGTGCA GACTGGGGCT CCAGGTTTCT TCCCCAGTGC AAGTCCCTCA
    GAATATGGCA CATATGCTGG AGCATATTAC CCTGCACAAC AGACGTTCCC TCCTGGAGTG
    CCTGGAGCAC AAGTTATGAT GAATCAGCCA CAGGTGATCC CTCCTAAACG GGAGCGCAAA
    ACGATAAGAA TTAGAGACCC CAACCAAGGA GGCAAAGATA TCACAGAAGA AATTCTCTCA
    GGAGGAAGAA CATCCTCTAC GCCAACGCCT CCACAGTCAA CTGTGGGAAG TTCTGAAATT
    CAAGCTAATG GAGAAAGCGT TCATCATGTT ACCGTTGTTG TGCGCCCAGA TGATGGACCC
    AAGGCCACCC AAGTGCTGAT CAAAGCCTCA GAGAGCCCTT CCCCTCCTCT GCCTCTCTCT
    GAAGCTGAGC CAAGCATAGC AAATGTGTGT GATATTTCAA AAGATAACAC TATCTCTGAG
    TTGACTTCTC ATATAGTAGA GCCCCTAAGT TCAGAAGAGA CAACCATTGA TACTTCTACC
    TCTCAGGTGG ATCTCAAACA CCAGCCAGTG GCTGCTATGG CAGAGCATGA AACTCTTATA
    GAGGAGGCAT CAATACAGGA GGTGGCCTTT GCAGAGGCAC CCATCGCTCA ATCAGAGATA
    CCAAAATCTC TTGCCTCCCA GCCTCTAGCA GAAATCTCTA CCCCTGAACA GATTTCTAAA
    CTTGACGCAC AAGTGCCATC TTTGCAACAA GAGGAGTTAC TTACAGAAAC TGTTGAACTT
    AATTTAAGCA ATCAGGCTCT TCCTTCACAA ATTTCTGAGC CTGAACCTTT TGAACCTGAG
    GCTTCAGCTT CAGATCCTCT ACATGAGGAG CCCACAGAGC CCACAGTTTC AACAGAAGAA
    CCACAAGCTA ATGGACTGTC TGAGGAGACA CCAGTATTAC CTGTACAGCC ACAACCAACA
    TCTGTTTGCC AGACTGAGCC CTCCCTAGAA TCCCCCATCG CTCAGCCTGA GGAGCTGCTT
    CTAACAAATG GAGGTGGACC ACCTGGCCTA CAAGAAAATG ATGCACCTGA TTCACAGGTT
    GAAGCTCATG TTAGCCCAAT CACTGAGCCT GAGGACCAAC CTGTGACAGT CTCTGATAAC
    GTGCTTGTGG AAGAGGATGT AGAAAAAACA TTGCCTGCGG ATACGGAAGT CGTGGCTTCA
    ACCCAAGTTG TAAAGCAGAG CTCCGAGAGT CAGTCTCAGG TTGCTGTTGC TGCACCCAAG
    AAAAAGCGAA AAATGAAGGA ACTAAACAAG AAAGAGGCAG TGGGAGATCT CCTTGATGCG
    TTCAAAGAGG CTGGTGATGG CTCTTCTGAT GTGGATTCTT CCTCTAAGCC TACCAGTGCT
    GCACCTGAAC CAGATCAGAG CCCTTGTGCT CCTCCTGAGG AACAGGATGA AACTTGGGAA
    GAGAAGGAAG ATAAACTGGA TGCAGAGAAT ATTAAACCAG GAGAACATAA ATATAAATAC
    AAAGAAGAGC AATGGAAACC CCTAAACCCG GAGGAAAAGA AGAGTTATGA TCGCGAGTTT
    CTGCTAGGGT TCCAGTTTAT TGTGGCCAGT ATGCAGAAAC CTGCGGGTCT TCCTCAAATT
    ACTGATGTGG TGCTGGATAA GGCAAACAAA ACTCCATTGC GACCATTGGA TCCTACACGT
    CTGACGGGAA TGAACTGTGG GCCAGATTTT ACACCTTCAT TTGCCAACCT TGGTCGGCCT
    GCTGTGGGTA ACCGTGGACC ACCAGCTGGC ATGGGTCCGA GACGGTCCCA GCAGGGTCCA
    CGAAAAGAGC CTCGTAAAAT TATTCAAACC GTATCATTAA ATGAGAATGT ACAACTAAAC
    AAAGCTGAAA AGGCATGGAA ACCGATAAAC AAGAGAGCTG GCGATGAGGA GGACCCAGAA
    AATATTAAGA CACAGGACCT ATTCCGAAGG GTTCGCAGTA TCTTGAATAA GCTGACCCCT
    CAGATGTTCC AGCAGCTGAT GAAGCAGGTC ACTGACCTTA CTATTGATAC CGAAGATCGT
    CTTAAGGGTG TCATTGACCT AATTTTTGAA AAAGCCATTT CAGAACCCAA TTTCTCTGTT
    GCATATGCCA ACATGTGTCG TTGCCTTATG GGGCTTAAAG TTCCAACCAC AGACAAGCCA
    GGGGTTACAG TCAACTTCCG CAAGTTGTTA CTGAATCGGT GCCAGAAGGA GTTTGAGAAG
    GATAAGGATG ATGATGAAGT GTTTGAGAAG AAACAAAAAG AAATGGATGC TGCTGCTTCG
    CCGGAGGAAA AAGCACGTTT GAATGATGAA CTGGTCGAGG CCCGTGACAG TGCACGACGG
    CGATCCTTGG GGAACATTAA GTTCATTGGA GAACTGTTTA AGTTAAAAAT GCTGACTGAA
    GCTATAATGC ACGATTGTGT GGTGAAGCTA TTAAAAAATC ATGATGAGGA ATCTCTGGAG
    TGCCTCTGCC GACTGCTGTC CACCATTGGC AAGGACCTGG ATTTTGAGAG AGCTAAGCCA
    CGTATGGACC AGTACTTCAA CCAGATGGAT AAAATCATTA AGGAGCGGAA GACATCTTCA
    CGAATTAGAT TCATGCTGCA GGATGTGATT GATTTGCGTC TGTGTAATTG GGTGCCTCGT
    CGTGCAGATC AGGGCCCTAA AACTATAGAT CAGATTCATA AAGAAGCAGA AATGGAAAAG
    CAGAGGGAGC AGGTTAAGGT GCAACAACTG ATGTCTAAAC AAGACAAGCG GCGAGAGCCC
    CCAGGACGAC CCACTGGAAG CCGGGGTAAC CAGCCTGCAG ATGATGGCTG GAATACAGTG
    CCCATCAGTA AGGGCAGCCG TCCTATTGAC ACCACTAGGA TCACAAAGAT AACAAAGCTT
    GGTGCTATTG ATTCAAATCA ACTGCTTGCC CCAGGAGGAC GTCTGAGTTG GGGTAAAGGA
    AGCAGCGGAG GAACTGGAGC CAAACCAGCA GAAACAGCTC CAGAATCTAG TCGGCCTGCG
    ACCAGCACCT TAAACAGGTT TTCTGCCTTG CAGCAGTCTT CATCTGCTGA GAGTCAAGAT
    GCTAGACGGC CCTTGCATCG GGGAAGTTCT AGTCGGGAAC GTTCTGAAAA AGATCGTGGA
    GACAGATTTG ATAGAGGTGA AAGGTTTGAT AAGGGAGAAC TGTTTGATAG ATCTGACCGT
    TCAGATCGAG CTCGTCCTCT TCTTACAAAG AGAAGCTATA GCAAGGAAAC TGAGGACCGT
    AGCCGAGAAA GAGAGGCCCA TATACCACAG GAGGCTGTGA GGAAGGTGGC AAGTATGACT
    GAGCAGCGGG ACCGCAGCAA AGAAAGTGTG AAAAAAGAAA TTCCAGCACC GACAGCAGTG
    GTTGCCTCAT CAAAGCCCTC TATGTCTGAG GATGAGCTAG AGAAGAAATC TAAAGCCATC
    ATAGAAGAAT ATTTGCACAT TAATGATATG AAGGAAGCTC TTCAGTGTGT AGTGGAGCTT
    AATAGTTCAA CGCTGCTTTT TATCTTTGTG CGGAATGGGA TTGAGTCAAC ACTAGAGAGA
    AGCACTATTG CCAGAGAGCA TATGGGTCTC CTTTTGTACC AGTTAGTAAA GGCAGGAACT
    CTGTCCAAGG AGCAGTATTA CAAAGGGGTG CTTGAAGTTC TTGAAGTAGC AGAAGATATG
    GAAATAGATA TACCCCACAT CTGGCTGTAT TTAGCAGAGA TAATCAGCCC TGTGCTGCTA
    GAGGGGGGGA TTCCGATGGG GGAGCTGTTC AGAGAGCTGA CCAAACCTTT AGTGCTCATT
    GGCAAAGCTG GAGTCCTTCT GGCAGAAATC CTTGGATTAT TGTGCAAAGG CATGAGTCAT
    AAGAAGGCTG GAGCCCTGTG GAAAGAATCT GGGCTAAGCT GGAAGGAATT CTTGTCCAAG
    GACCAGGATA TTAACAAGTT TATAACTGAC AAGAAACTCG AATACACAAC AGGAGAGGAG
    ACTGAAACAC CCAGTAAGAA AGACCTCACC CCTGCTGAAC TTGATAAAAT TTTGACAAAA
    CTTTTGAAAG AGAACAGCAG TAACCAGAAG ATATTTGACT GGATTGAAGC CAACTTAAAT
    GACCAGCAAA CTTCATCCAA CACCTTTGTT CGAACTTTAA TGAGTACTGT GTGCCACTCT
    GCTATTATTT TTGAGAATCC TTGTAGGGTT GATGTTAATA TTATCCATAC TAGAGCCAAA
    TTTCTTCAGA AATATTTAAG TGACGAGGCC AAGGAACTAC AGGCTCTCTA TGCTCTACAG
    GCACTTGTTG TAAAGCTTGA CCAGCCCCCA AATCTCCTAC GGATGTTTTT TGATGCTCTC
    TATGATGAGG ATGTGATAAA AGAGGATCCA TTCTACAAGT GGGAGTCCAG CAAAGACCCA
    GCTGAACAGC AAGGAAAAGG TGTTGCTCTT AAATCTGTCA CTGCCTTCTT CACATGGCTA
    CGGGAAGCAG AGGATGAGTC TGATAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012818831.1
    CDS130..4959
    Translation

    Target ORF information:

    RefSeq Version XM_012963377.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012963377.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
    CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA
    CAAATGAATA CACAATCTCA GCCTCGACAG GCAGGATTCA GATCAATCCA GCATTATTAT
    CAAAATAGGA CACAGCCTCC CACTAGTGCC ACCAGAGTGC AGAGTGGTTC TTCTGCTCGA
    GCAGTTACTC CAGCCCATGT GTATCAACCA GGCTCCCAAG TTATGATGAT TCCTTCACAG
    ATTTCATACC CTTCCCAGGG ATACTACATT CAGGGACAGG GACGCTCATA TGTGGTGCCC
    ACAAACCAGT ACCCTGTGCA GACTGGGGCT CCAGGTTTCT TCCCCAGTGC AAGTCCCTCA
    GAATATGGCA CATATGCTGG AGCATATTAC CCTGCACAAC AGACGTTCCC TCCTGGAGTG
    CCTGGAGCAC AAGTTATGAT GAATCAGCCA CAGGTGATCC CTCCTAAACG GGAGCGCAAA
    ACGATAAGAA TTAGAGACCC CAACCAAGGA GGCAAAGATA TCACAGAAGA AATTCTCTCA
    GGAGGAAGAA CATCCTCTAC GCCAACGCCT CCACAGTCAA CTGTGGGAAG TTCTGAAATT
    CAAGCTAATG GAGAAAGCGT TCATCATGTT ACCGTTGTTG TGCGCCCAGA TGATGGACCC
    AAGGCCACCC AAGTGCTGAT CAAAGCCTCA GAGAGCCCTT CCCCTCCTCT GCCTCTCTCT
    GAAGCTGAGC CAAGCATAGC AAATGTGTGT GATATTTCAA AAGATAACAC TATCTCTGAG
    TTGACTTCTC ATATAGTAGA GCCCCTAAGT TCAGAAGAGA CAACCATTGA TACTTCTACC
    TCTCAGGTGG ATCTCAAACA CCAGCCAGTG GCTGCTATGG CAGAGCATGA AACTCTTATA
    GAGGAGGCAT CAATACAGGA GGTGGCCTTT GCAGAGGCAC CCATCGCTCA ATCAGAGATA
    CCAAAATCTC TTGCCTCCCA GCCTCTAGCA GAAATCTCTA CCCCTGAACA GATTTCTAAA
    CTTGACGCAC AAGTGCCATC TTTGCAACAA GAGGAGTTAC TTACAGAAAC TGTTGAACTT
    AATTTAAGCA ATCAGGCTCT TCCTTCACAA ATTTCTGAGC CTGAACCTTT TGAACCTGAG
    GCTTCAGCTT CAGATCCTCT ACATGAGGAG CCCACAGAGC CCACAGTTTC AACAGAAGAA
    CCACAAGCTA ATGGACTGTC TGAGGAGACA CCAGTATTAC CTGTACAGCC ACAACCAACA
    TCTGTTTGCC AGACTGAGCC CTCCCTAGAA TCCCCCATCG CTCAGCCTGA GGAGCTGCTT
    CTAACAAATG GAGGTGGACC ACCTGGCCTA CAAGAAAATG ATGCACCTGA TTCACAGGTT
    GAAGCTCATG TTAGCCCAAT CACTGAGCCT GAGGACCAAC CTGTGACAGT CTCTGATAAC
    GTGCTTGTGG AAGAGGATGT AGAAAAAACA TTGCCTGCGG ATACGGAAGT CGTGGCTTCA
    ACCCAAGTTG TAAAGCAGAG CTCCGAGAGT CAGTCTCAGG TTGCTGTTGC TGCACCCAAG
    AAAAAGCGAA AAATGAAGGA ACTAAACAAG AAAGAGGCAG TGGGAGATCT CCTTGATGCG
    TTCAAAGAGG CTGGTGATGG CTCTTCTGAT GTGGATTCTT CCTCTAAGCC TACCAGTGCT
    GCACCTGAAC CAGATCAGAG CCCTTGTGCT CCTCCTGAGG AACAGGATGA AACTTGGGAA
    GAGAAGGAAG ATAAACTGGA TGCAGAGAAT ATTAAACCAG GAGAACATAA ATATAAATAC
    AAAGAAGAGC AATGGAAACC CCTAAACCCG GAGGAAAAGA AGAGTTATGA TCGCGAGTTT
    CTGCTAGGGT TCCAGTTTAT TGTGGCCAGT ATGCAGAAAC CTGCGGGTCT TCCTCAAATT
    ACTGATGTGG TGCTGGATAA GGCAAACAAA ACTCCATTGC GACCATTGGA TCCTACACGT
    CTGACGGGAA TGAACTGTGG GCCAGATTTT ACACCTTCAT TTGCCAACCT TGGTCGGCCT
    GCTGTGGGTA ACCGTGGACC ACCAGCTGGC ATGGGTCCGA GACGGTCCCA GCAGGGTCCA
    CGAAAAGAGC CTCGTAAAAT TATTCAAACC GTATCATTAA ATGAGAATGT ACAACTAAAC
    AAAGCTGAAA AGGCATGGAA ACCGATAAAC AAGAGAGCTG GCGATGAGGA GGACCCAGAA
    AATATTAAGA CACAGGACCT ATTCCGAAGG GTTCGCAGTA TCTTGAATAA GCTGACCCCT
    CAGATGTTCC AGCAGCTGAT GAAGCAGGTC ACTGACCTTA CTATTGATAC CGAAGATCGT
    CTTAAGGGTG TCATTGACCT AATTTTTGAA AAAGCCATTT CAGAACCCAA TTTCTCTGTT
    GCATATGCCA ACATGTGTCG TTGCCTTATG GGGCTTAAAG TTCCAACCAC AGACAAGCCA
    GGGGTTACAG TCAACTTCCG CAAGTTGTTA CTGAATCGGT GCCAGAAGGA GTTTGAGAAG
    GATAAGGATG ATGATGAAGT GTTTGAGAAG AAACAAAAAG AAATGGATGC TGCTGCTTCG
    CCGGAGGAAA AAGCACGTTT GAATGATGAA CTGGTCGAGG CCCGTGACAG TGCACGACGG
    CGATCCTTGG GGAACATTAA GTTCATTGGA GAACTGTTTA AGTTAAAAAT GCTGACTGAA
    GCTATAATGC ACGATTGTGT GGTGAAGCTA TTAAAAAATC ATGATGAGGA ATCTCTGGAG
    TGCCTCTGCC GACTGCTGTC CACCATTGGC AAGGACCTGG ATTTTGAGAG AGCTAAGCCA
    CGTATGGACC AGTACTTCAA CCAGATGGAT AAAATCATTA AGGAGCGGAA GACATCTTCA
    CGAATTAGAT TCATGCTGCA GGATGTGATT GATTTGCGTC TGTGTAATTG GGTGCCTCGT
    CGTGCAGATC AGGGCCCTAA AACTATAGAT CAGATTCATA AAGAAGCAGA AATGGAAAAG
    CAGAGGGAGC AGGTTAAGGT GCAACAACTG ATGTCTAAAC AAGACAAGCG GCGAGAGCCC
    CCAGGACGAC CCACTGGAAG CCGGGGTAAC CAGCCTGCAG ATGATGGCTG GAATACAGTG
    CCCATCAGTA AGGGCAGCCG TCCTATTGAC ACCACTAGGA TCACAAAGAT AACAAAGCTT
    GGTGCTATTG ATTCAAATCA ACTGCTTGCC CCAGGAGGAC GTCTGAGTTG GGGTAAAGGA
    AGCAGCGGAG GAACTGGAGC CAAACCAGCA GAAACAGCTC CAGAATCTAG TCGGCCTGCG
    ACCAGCACCT TAAACAGGTT TTCTGCCTTG CAGCAGTCTT CATCTGCTGA GAGTCAAGAT
    GCTAGACGGC CCTTGCATCG GGGAAGTTCT AGTCGGGAAC GTTCTGAAAA AGATCGTGGA
    GACAGATTTG ATAGAGGTGA AAGGTTTGAT AAGGGAGAAC TGTTTGATAG ATCTGACCGT
    TCAGATCGAG CTCGTCCTCT TCTTACAAAG AGAAGCTATA GCAAGGAAAC TGAGGACCGT
    AGCCGAGAAA GAGAGGCCCA TATACCACAG GAGGCTGTGA GGAAGGTGGC AAGTATGACT
    GAGCAGCGGG ACCGCAGCAA AGAAAGTGTG AAAAAAGAAA TTCCAGCACC GACAGCAGTG
    GTTGCCTCAT CAAAGCCCTC TATGTCTGAG GATGAGCTAG AGAAGAAATC TAAAGCCATC
    ATAGAAGAAT ATTTGCACAT TAATGATATG AAGGAAGCTC TTCAGTGTGT AGTGGAGCTT
    AATAGTTCAA CGCTGCTTTT TATCTTTGTG CGGAATGGGA TTGAGTCAAC ACTAGAGAGA
    AGCACTATTG CCAGAGAGCA TATGGGTCTC CTTTTGTACC AGTTAGTAAA GGCAGGAACT
    CTGTCCAAGG AGCAGTATTA CAAAGGGGTG CTTGAAGTTC TTGAAGTAGC AGAAGATATG
    GAAATAGATA TACCCCACAT CTGGCTGTAT TTAGCAGAGA TAATCAGCCC TGTGCTGCTA
    GAGGGGGGGA TTCCGATGGG GGAGCTGTTC AGAGAGCTGA CCAAACCTTT AGTGCTCATT
    GGCAAAGCTG GAGTCCTTCT GGCAGAAATC CTTGGATTAT TGTGCAAAGG CATGAGTCAT
    AAGAAGGCTG GAGCCCTGTG GAAAGAATCT GGGCTAAGCT GGAAGGAATT CTTGTCCAAG
    GACCAGGATA TTAACAAGTT TATAACTGAC AAGAAACTCG AATACACAAC AGGAGAGGAG
    ACTGAAACAC CCAGTAAGAA AGACCTCACC CCTGCTGAAC TTGATAAAAT TTTGACAAAA
    CTTTTGAAAG AGAACAGCAG TAACCAGAAG ATATTTGACT GGATTGAAGC CAACTTAAAT
    GACCAGCAAA CTTCATCCAA CACCTTTGTT CGAACTTTAA TGAGTACTGT GTGCCACTCT
    GCTATTATTT TTGAGAATCC TTGTAGGGTT GATGTTAATA TTATCCATAC TAGAGCCAAA
    TTTCTTCAGA AATATTTAAG TGACGAGGCC AAGGAACTAC AGGCTCTCTA TGCTCTACAG
    GCACTTGTTG TAAAGCTTGA CCAGCCCCCA AATCTCCTAC GGATGTTTTT TGATGCTCTC
    TATGATGAGG ATGTGATAAA AGAGGATCCA TTCTACAAGT GGGAGTCCAG CAAAGACCCA
    GCTGAACAGC AAGGAAAAGG TGTTGCTCTT AAATCTGTCA CTGCCTTCTT CACATGGCTA
    CGGGAAGCAG AGGATGAGTC TGATAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa10205
    Clone ID Related Accession (Same CDS sequence) XM_004914401.4 , XM_004914400.3 , XM_004914401.3 , XM_012963378.2 , XM_012963378.3 , XM_004914400.4
    Accession Version XM_004914401.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4809bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004914401.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
    CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA
    CAAATGAATA CACAATCTCA GCCTCGACAG CATTATTATC AAAATAGGAC ACAGCCTCCC
    ACTAGTGCCA CCAGAGTGCA GAGTGGTTCT TCTGCTCGAG CAGTTACTCC AGCCCATGTG
    TATCAACCAG GCTCCCAAGT TATGATGATT CCTTCACAGA TTTCATACCC TTCCCAGGGA
    TACTACATTC AGGGACAGGG ACGCTCATAT GTGGTGCCCA CAAACCAGTA CCCTGTGCAG
    ACTGGGGCTC CAGGTTTCTT CCCCAGTGCA AGTCCCTCAG AATATGGCAC ATATGCTGGA
    GCATATTACC CTGCACAACA GACGTTCCCT CCTGGAGTGC CTGGAGCACA AGTTATGATG
    AATCAGCCAC AGGTGATCCC TCCTAAACGG GAGCGCAAAA CGATAAGAAT TAGAGACCCC
    AACCAAGGAG GCAAAGATAT CACAGAAGAA ATTCTCTCAG GAGGAAGAAC ATCCTCTACG
    CCAACGCCTC CACAGTCAAC TGTGGGAAGT TCTGAAATTC AAGCTAATGG AGAAAGCGTT
    CATCATGTTA CCGTTGTTGT GCGCCCAGAT GATGGACCCA AGGCCACCCA AGTGCTGATC
    AAAGCCTCAG AGAGCCCTTC CCCTCCTCTG CCTCTCTCTG AAGCTGAGCC AAGCATAGCA
    AATGTGTGTG ATATTTCAAA AGATAACACT ATCTCTGAGT TGACTTCTCA TATAGTAGAG
    CCCCTAAGTT CAGAAGAGAC AACCATTGAT ACTTCTACCT CTCAGGTGGA TCTCAAACAC
    CAGCCAGTGG CTGCTATGGC AGAGCATGAA ACTCTTATAG AGGAGGCATC AATACAGGAG
    GTGGCCTTTG CAGAGGCACC CATCGCTCAA TCAGAGATAC CAAAATCTCT TGCCTCCCAG
    CCTCTAGCAG AAATCTCTAC CCCTGAACAG ATTTCTAAAC TTGACGCACA AGTGCCATCT
    TTGCAACAAG AGGAGTTACT TACAGAAACT GTTGAACTTA ATTTAAGCAA TCAGGCTCTT
    CCTTCACAAA TTTCTGAGCC TGAACCTTTT GAACCTGAGG CTTCAGCTTC AGATCCTCTA
    CATGAGGAGC CCACAGAGCC CACAGTTTCA ACAGAAGAAC CACAAGCTAA TGGACTGTCT
    GAGGAGACAC CAGTATTACC TGTACAGCCA CAACCAACAT CTGTTTGCCA GACTGAGCCC
    TCCCTAGAAT CCCCCATCGC TCAGCCTGAG GAGCTGCTTC TAACAAATGG AGGTGGACCA
    CCTGGCCTAC AAGAAAATGA TGCACCTGAT TCACAGGTTG AAGCTCATGT TAGCCCAATC
    ACTGAGCCTG AGGACCAACC TGTGACAGTC TCTGATAACG TGCTTGTGGA AGAGGATGTA
    GAAAAAACAT TGCCTGCGGA TACGGAAGTC GTGGCTTCAA CCCAAGTTGT AAAGCAGAGC
    TCCGAGAGTC AGTCTCAGGT TGCTGTTGCT GCACCCAAGA AAAAGCGAAA AATGAAGGAA
    CTAAACAAGA AAGAGGCAGT GGGAGATCTC CTTGATGCGT TCAAAGAGGC TGGTGATGGC
    TCTTCTGATG TGGATTCTTC CTCTAAGCCT ACCAGTGCTG CACCTGAACC AGATCAGAGC
    CCTTGTGCTC CTCCTGAGGA ACAGGATGAA ACTTGGGAAG AGAAGGAAGA TAAACTGGAT
    GCAGAGAATA TTAAACCAGG AGAACATAAA TATAAATACA AAGAAGAGCA ATGGAAACCC
    CTAAACCCGG AGGAAAAGAA GAGTTATGAT CGCGAGTTTC TGCTAGGGTT CCAGTTTATT
    GTGGCCAGTA TGCAGAAACC TGCGGGTCTT CCTCAAATTA CTGATGTGGT GCTGGATAAG
    GCAAACAAAA CTCCATTGCG ACCATTGGAT CCTACACGTC TGACGGGAAT GAACTGTGGG
    CCAGATTTTA CACCTTCATT TGCCAACCTT GGTCGGCCTG CTGTGGGTAA CCGTGGACCA
    CCAGCTGGCA TGGGTCCGAG ACGGTCCCAG CAGGGTCCAC GAAAAGAGCC TCGTAAAATT
    ATTCAAACCG TATCATTAAA TGAGAATGTA CAACTAAACA AAGCTGAAAA GGCATGGAAA
    CCGATAAACA AGAGAGCTGG CGATGAGGAG GACCCAGAAA ATATTAAGAC ACAGGACCTA
    TTCCGAAGGG TTCGCAGTAT CTTGAATAAG CTGACCCCTC AGATGTTCCA GCAGCTGATG
    AAGCAGGTCA CTGACCTTAC TATTGATACC GAAGATCGTC TTAAGGGTGT CATTGACCTA
    ATTTTTGAAA AAGCCATTTC AGAACCCAAT TTCTCTGTTG CATATGCCAA CATGTGTCGT
    TGCCTTATGG GGCTTAAAGT TCCAACCACA GACAAGCCAG GGGTTACAGT CAACTTCCGC
    AAGTTGTTAC TGAATCGGTG CCAGAAGGAG TTTGAGAAGG ATAAGGATGA TGATGAAGTG
    TTTGAGAAGA AACAAAAAGA AATGGATGCT GCTGCTTCGC CGGAGGAAAA AGCACGTTTG
    AATGATGAAC TGGTCGAGGC CCGTGACAGT GCACGACGGC GATCCTTGGG GAACATTAAG
    TTCATTGGAG AACTGTTTAA GTTAAAAATG CTGACTGAAG CTATAATGCA CGATTGTGTG
    GTGAAGCTAT TAAAAAATCA TGATGAGGAA TCTCTGGAGT GCCTCTGCCG ACTGCTGTCC
    ACCATTGGCA AGGACCTGGA TTTTGAGAGA GCTAAGCCAC GTATGGACCA GTACTTCAAC
    CAGATGGATA AAATCATTAA GGAGCGGAAG ACATCTTCAC GAATTAGATT CATGCTGCAG
    GATGTGATTG ATTTGCGTCT GTGTAATTGG GTGCCTCGTC GTGCAGATCA GGGCCCTAAA
    ACTATAGATC AGATTCATAA AGAAGCAGAA ATGGAAAAGC AGAGGGAGCA GGTTAAGGTG
    CAACAACTGA TGTCTAAACA AGACAAGCGG CGAGAGCCCC CAGGACGACC CACTGGAAGC
    CGGGGTAACC AGCCTGCAGA TGATGGCTGG AATACAGTGC CCATCAGTAA GGGCAGCCGT
    CCTATTGACA CCACTAGGAT CACAAAGATA ACAAAGCTTG GTGCTATTGA TTCAAATCAA
    CTGCTTGCCC CAGGAGGACG TCTGAGTTGG GGTAAAGGAA GCAGCGGAGG AACTGGAGCC
    AAACCAGCAG AAACAGCTCC AGAATCTAGT CGGCCTGCGA CCAGCACCTT AAACAGGTTT
    TCTGCCTTGC AGCAGTCTTC ATCTGCTGAG AGTCAAGATG CTAGACGGCC CTTGCATCGG
    GGAAGTTCTA GTCGGGAACG TTCTGAAAAA GATCGTGGAG ACAGATTTGA TAGAGGTGAA
    AGGTTTGATA AGGGAGAACT GTTTGATAGA TCTGACCGTT CAGATCGAGC TCGTCCTCTT
    CTTACAAAGA GAAGCTATAG CAAGGAAACT GAGGACCGTA GCCGAGAAAG AGAGGCCCAT
    ATACCACAGG AGGCTGTGAG GAAGGTGGCA AGTATGACTG AGCAGCGGGA CCGCAGCAAA
    GAAAGTGTGA AAAAAGAAAT TCCAGCACCG ACAGCAGTGG TTGCCTCATC AAAGCCCTCT
    ATGTCTGAGG ATGAGCTAGA GAAGAAATCT AAAGCCATCA TAGAAGAATA TTTGCACATT
    AATGATATGA AGGAAGCTCT TCAGTGTGTA GTGGAGCTTA ATAGTTCAAC GCTGCTTTTT
    ATCTTTGTGC GGAATGGGAT TGAGTCAACA CTAGAGAGAA GCACTATTGC CAGAGAGCAT
    ATGGGTCTCC TTTTGTACCA GTTAGTAAAG GCAGGAACTC TGTCCAAGGA GCAGTATTAC
    AAAGGGGTGC TTGAAGTTCT TGAAGTAGCA GAAGATATGG AAATAGATAT ACCCCACATC
    TGGCTGTATT TAGCAGAGAT AATCAGCCCT GTGCTGCTAG AGGGGGGGAT TCCGATGGGG
    GAGCTGTTCA GAGAGCTGAC CAAACCTTTA GTGCTCATTG GCAAAGCTGG AGTCCTTCTG
    GCAGAAATCC TTGGATTATT GTGCAAAGGC ATGAGTCATA AGAAGGCTGG AGCCCTGTGG
    AAAGAATCTG GGCTAAGCTG GAAGGAATTC TTGTCCAAGG ACCAGGATAT TAACAAGTTT
    ATAACTGACA AGAAACTCGA ATACACAACA GGAGAGGAGA CTGAAACACC CAGTAAGAAA
    GACCTCACCC CTGCTGAACT TGATAAAATT TTGACAAAAC TTTTGAAAGA GAACAGCAGT
    AACCAGAAGA TATTTGACTG GATTGAAGCC AACTTAAATG ACCAGCAAAC TTCATCCAAC
    ACCTTTGTTC GAACTTTAAT GAGTACTGTG TGCCACTCTG CTATTATTTT TGAGAATCCT
    TGTAGGGTTG ATGTTAATAT TATCCATACT AGAGCCAAAT TTCTTCAGAA ATATTTAAGT
    GACGAGGCCA AGGAACTACA GGCTCTCTAT GCTCTACAGG CACTTGTTGT AAAGCTTGAC
    CAGCCCCCAA ATCTCCTACG GATGTTTTTT GATGCTCTCT ATGATGAGGA TGTGATAAAA
    GAGGATCCAT TCTACAAGTG GGAGTCCAGC AAAGACCCAG CTGAACAGCA AGGAAAAGGT
    GTTGCTCTTA AATCTGTCAC TGCCTTCTTC ACATGGCTAC GGGAAGCAGA GGATGAGTCT
    GATAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004914458.1
    CDS146..4954
    Translation

    Target ORF information:

    RefSeq Version XM_004914401.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004914401.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
    CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA
    CAAATGAATA CACAATCTCA GCCTCGACAG CATTATTATC AAAATAGGAC ACAGCCTCCC
    ACTAGTGCCA CCAGAGTGCA GAGTGGTTCT TCTGCTCGAG CAGTTACTCC AGCCCATGTG
    TATCAACCAG GCTCCCAAGT TATGATGATT CCTTCACAGA TTTCATACCC TTCCCAGGGA
    TACTACATTC AGGGACAGGG ACGCTCATAT GTGGTGCCCA CAAACCAGTA CCCTGTGCAG
    ACTGGGGCTC CAGGTTTCTT CCCCAGTGCA AGTCCCTCAG AATATGGCAC ATATGCTGGA
    GCATATTACC CTGCACAACA GACGTTCCCT CCTGGAGTGC CTGGAGCACA AGTTATGATG
    AATCAGCCAC AGGTGATCCC TCCTAAACGG GAGCGCAAAA CGATAAGAAT TAGAGACCCC
    AACCAAGGAG GCAAAGATAT CACAGAAGAA ATTCTCTCAG GAGGAAGAAC ATCCTCTACG
    CCAACGCCTC CACAGTCAAC TGTGGGAAGT TCTGAAATTC AAGCTAATGG AGAAAGCGTT
    CATCATGTTA CCGTTGTTGT GCGCCCAGAT GATGGACCCA AGGCCACCCA AGTGCTGATC
    AAAGCCTCAG AGAGCCCTTC CCCTCCTCTG CCTCTCTCTG AAGCTGAGCC AAGCATAGCA
    AATGTGTGTG ATATTTCAAA AGATAACACT ATCTCTGAGT TGACTTCTCA TATAGTAGAG
    CCCCTAAGTT CAGAAGAGAC AACCATTGAT ACTTCTACCT CTCAGGTGGA TCTCAAACAC
    CAGCCAGTGG CTGCTATGGC AGAGCATGAA ACTCTTATAG AGGAGGCATC AATACAGGAG
    GTGGCCTTTG CAGAGGCACC CATCGCTCAA TCAGAGATAC CAAAATCTCT TGCCTCCCAG
    CCTCTAGCAG AAATCTCTAC CCCTGAACAG ATTTCTAAAC TTGACGCACA AGTGCCATCT
    TTGCAACAAG AGGAGTTACT TACAGAAACT GTTGAACTTA ATTTAAGCAA TCAGGCTCTT
    CCTTCACAAA TTTCTGAGCC TGAACCTTTT GAACCTGAGG CTTCAGCTTC AGATCCTCTA
    CATGAGGAGC CCACAGAGCC CACAGTTTCA ACAGAAGAAC CACAAGCTAA TGGACTGTCT
    GAGGAGACAC CAGTATTACC TGTACAGCCA CAACCAACAT CTGTTTGCCA GACTGAGCCC
    TCCCTAGAAT CCCCCATCGC TCAGCCTGAG GAGCTGCTTC TAACAAATGG AGGTGGACCA
    CCTGGCCTAC AAGAAAATGA TGCACCTGAT TCACAGGTTG AAGCTCATGT TAGCCCAATC
    ACTGAGCCTG AGGACCAACC TGTGACAGTC TCTGATAACG TGCTTGTGGA AGAGGATGTA
    GAAAAAACAT TGCCTGCGGA TACGGAAGTC GTGGCTTCAA CCCAAGTTGT AAAGCAGAGC
    TCCGAGAGTC AGTCTCAGGT TGCTGTTGCT GCACCCAAGA AAAAGCGAAA AATGAAGGAA
    CTAAACAAGA AAGAGGCAGT GGGAGATCTC CTTGATGCGT TCAAAGAGGC TGGTGATGGC
    TCTTCTGATG TGGATTCTTC CTCTAAGCCT ACCAGTGCTG CACCTGAACC AGATCAGAGC
    CCTTGTGCTC CTCCTGAGGA ACAGGATGAA ACTTGGGAAG AGAAGGAAGA TAAACTGGAT
    GCAGAGAATA TTAAACCAGG AGAACATAAA TATAAATACA AAGAAGAGCA ATGGAAACCC
    CTAAACCCGG AGGAAAAGAA GAGTTATGAT CGCGAGTTTC TGCTAGGGTT CCAGTTTATT
    GTGGCCAGTA TGCAGAAACC TGCGGGTCTT CCTCAAATTA CTGATGTGGT GCTGGATAAG
    GCAAACAAAA CTCCATTGCG ACCATTGGAT CCTACACGTC TGACGGGAAT GAACTGTGGG
    CCAGATTTTA CACCTTCATT TGCCAACCTT GGTCGGCCTG CTGTGGGTAA CCGTGGACCA
    CCAGCTGGCA TGGGTCCGAG ACGGTCCCAG CAGGGTCCAC GAAAAGAGCC TCGTAAAATT
    ATTCAAACCG TATCATTAAA TGAGAATGTA CAACTAAACA AAGCTGAAAA GGCATGGAAA
    CCGATAAACA AGAGAGCTGG CGATGAGGAG GACCCAGAAA ATATTAAGAC ACAGGACCTA
    TTCCGAAGGG TTCGCAGTAT CTTGAATAAG CTGACCCCTC AGATGTTCCA GCAGCTGATG
    AAGCAGGTCA CTGACCTTAC TATTGATACC GAAGATCGTC TTAAGGGTGT CATTGACCTA
    ATTTTTGAAA AAGCCATTTC AGAACCCAAT TTCTCTGTTG CATATGCCAA CATGTGTCGT
    TGCCTTATGG GGCTTAAAGT TCCAACCACA GACAAGCCAG GGGTTACAGT CAACTTCCGC
    AAGTTGTTAC TGAATCGGTG CCAGAAGGAG TTTGAGAAGG ATAAGGATGA TGATGAAGTG
    TTTGAGAAGA AACAAAAAGA AATGGATGCT GCTGCTTCGC CGGAGGAAAA AGCACGTTTG
    AATGATGAAC TGGTCGAGGC CCGTGACAGT GCACGACGGC GATCCTTGGG GAACATTAAG
    TTCATTGGAG AACTGTTTAA GTTAAAAATG CTGACTGAAG CTATAATGCA CGATTGTGTG
    GTGAAGCTAT TAAAAAATCA TGATGAGGAA TCTCTGGAGT GCCTCTGCCG ACTGCTGTCC
    ACCATTGGCA AGGACCTGGA TTTTGAGAGA GCTAAGCCAC GTATGGACCA GTACTTCAAC
    CAGATGGATA AAATCATTAA GGAGCGGAAG ACATCTTCAC GAATTAGATT CATGCTGCAG
    GATGTGATTG ATTTGCGTCT GTGTAATTGG GTGCCTCGTC GTGCAGATCA GGGCCCTAAA
    ACTATAGATC AGATTCATAA AGAAGCAGAA ATGGAAAAGC AGAGGGAGCA GGTTAAGGTG
    CAACAACTGA TGTCTAAACA AGACAAGCGG CGAGAGCCCC CAGGACGACC CACTGGAAGC
    CGGGGTAACC AGCCTGCAGA TGATGGCTGG AATACAGTGC CCATCAGTAA GGGCAGCCGT
    CCTATTGACA CCACTAGGAT CACAAAGATA ACAAAGCTTG GTGCTATTGA TTCAAATCAA
    CTGCTTGCCC CAGGAGGACG TCTGAGTTGG GGTAAAGGAA GCAGCGGAGG AACTGGAGCC
    AAACCAGCAG AAACAGCTCC AGAATCTAGT CGGCCTGCGA CCAGCACCTT AAACAGGTTT
    TCTGCCTTGC AGCAGTCTTC ATCTGCTGAG AGTCAAGATG CTAGACGGCC CTTGCATCGG
    GGAAGTTCTA GTCGGGAACG TTCTGAAAAA GATCGTGGAG ACAGATTTGA TAGAGGTGAA
    AGGTTTGATA AGGGAGAACT GTTTGATAGA TCTGACCGTT CAGATCGAGC TCGTCCTCTT
    CTTACAAAGA GAAGCTATAG CAAGGAAACT GAGGACCGTA GCCGAGAAAG AGAGGCCCAT
    ATACCACAGG AGGCTGTGAG GAAGGTGGCA AGTATGACTG AGCAGCGGGA CCGCAGCAAA
    GAAAGTGTGA AAAAAGAAAT TCCAGCACCG ACAGCAGTGG TTGCCTCATC AAAGCCCTCT
    ATGTCTGAGG ATGAGCTAGA GAAGAAATCT AAAGCCATCA TAGAAGAATA TTTGCACATT
    AATGATATGA AGGAAGCTCT TCAGTGTGTA GTGGAGCTTA ATAGTTCAAC GCTGCTTTTT
    ATCTTTGTGC GGAATGGGAT TGAGTCAACA CTAGAGAGAA GCACTATTGC CAGAGAGCAT
    ATGGGTCTCC TTTTGTACCA GTTAGTAAAG GCAGGAACTC TGTCCAAGGA GCAGTATTAC
    AAAGGGGTGC TTGAAGTTCT TGAAGTAGCA GAAGATATGG AAATAGATAT ACCCCACATC
    TGGCTGTATT TAGCAGAGAT AATCAGCCCT GTGCTGCTAG AGGGGGGGAT TCCGATGGGG
    GAGCTGTTCA GAGAGCTGAC CAAACCTTTA GTGCTCATTG GCAAAGCTGG AGTCCTTCTG
    GCAGAAATCC TTGGATTATT GTGCAAAGGC ATGAGTCATA AGAAGGCTGG AGCCCTGTGG
    AAAGAATCTG GGCTAAGCTG GAAGGAATTC TTGTCCAAGG ACCAGGATAT TAACAAGTTT
    ATAACTGACA AGAAACTCGA ATACACAACA GGAGAGGAGA CTGAAACACC CAGTAAGAAA
    GACCTCACCC CTGCTGAACT TGATAAAATT TTGACAAAAC TTTTGAAAGA GAACAGCAGT
    AACCAGAAGA TATTTGACTG GATTGAAGCC AACTTAAATG ACCAGCAAAC TTCATCCAAC
    ACCTTTGTTC GAACTTTAAT GAGTACTGTG TGCCACTCTG CTATTATTTT TGAGAATCCT
    TGTAGGGTTG ATGTTAATAT TATCCATACT AGAGCCAAAT TTCTTCAGAA ATATTTAAGT
    GACGAGGCCA AGGAACTACA GGCTCTCTAT GCTCTACAGG CACTTGTTGT AAAGCTTGAC
    CAGCCCCCAA ATCTCCTACG GATGTTTTTT GATGCTCTCT ATGATGAGGA TGTGATAAAA
    GAGGATCCAT TCTACAAGTG GGAGTCCAGC AAAGACCCAG CTGAACAGCA AGGAAAAGGT
    GTTGCTCTTA AATCTGTCAC TGCCTTCTTC ACATGGCTAC GGGAAGCAGA GGATGAGTCT
    GATAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa10205
    Clone ID Related Accession (Same CDS sequence) XM_004914401.4 , XM_004914400.3 , XM_004914401.3 , XM_012963378.2 , XM_012963378.3 , XM_004914400.4
    Accession Version XM_012963378.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4809bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012963378.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
    CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA
    CAAATGAATA CACAATCTCA GCCTCGACAG CATTATTATC AAAATAGGAC ACAGCCTCCC
    ACTAGTGCCA CCAGAGTGCA GAGTGGTTCT TCTGCTCGAG CAGTTACTCC AGCCCATGTG
    TATCAACCAG GCTCCCAAGT TATGATGATT CCTTCACAGA TTTCATACCC TTCCCAGGGA
    TACTACATTC AGGGACAGGG ACGCTCATAT GTGGTGCCCA CAAACCAGTA CCCTGTGCAG
    ACTGGGGCTC CAGGTTTCTT CCCCAGTGCA AGTCCCTCAG AATATGGCAC ATATGCTGGA
    GCATATTACC CTGCACAACA GACGTTCCCT CCTGGAGTGC CTGGAGCACA AGTTATGATG
    AATCAGCCAC AGGTGATCCC TCCTAAACGG GAGCGCAAAA CGATAAGAAT TAGAGACCCC
    AACCAAGGAG GCAAAGATAT CACAGAAGAA ATTCTCTCAG GAGGAAGAAC ATCCTCTACG
    CCAACGCCTC CACAGTCAAC TGTGGGAAGT TCTGAAATTC AAGCTAATGG AGAAAGCGTT
    CATCATGTTA CCGTTGTTGT GCGCCCAGAT GATGGACCCA AGGCCACCCA AGTGCTGATC
    AAAGCCTCAG AGAGCCCTTC CCCTCCTCTG CCTCTCTCTG AAGCTGAGCC AAGCATAGCA
    AATGTGTGTG ATATTTCAAA AGATAACACT ATCTCTGAGT TGACTTCTCA TATAGTAGAG
    CCCCTAAGTT CAGAAGAGAC AACCATTGAT ACTTCTACCT CTCAGGTGGA TCTCAAACAC
    CAGCCAGTGG CTGCTATGGC AGAGCATGAA ACTCTTATAG AGGAGGCATC AATACAGGAG
    GTGGCCTTTG CAGAGGCACC CATCGCTCAA TCAGAGATAC CAAAATCTCT TGCCTCCCAG
    CCTCTAGCAG AAATCTCTAC CCCTGAACAG ATTTCTAAAC TTGACGCACA AGTGCCATCT
    TTGCAACAAG AGGAGTTACT TACAGAAACT GTTGAACTTA ATTTAAGCAA TCAGGCTCTT
    CCTTCACAAA TTTCTGAGCC TGAACCTTTT GAACCTGAGG CTTCAGCTTC AGATCCTCTA
    CATGAGGAGC CCACAGAGCC CACAGTTTCA ACAGAAGAAC CACAAGCTAA TGGACTGTCT
    GAGGAGACAC CAGTATTACC TGTACAGCCA CAACCAACAT CTGTTTGCCA GACTGAGCCC
    TCCCTAGAAT CCCCCATCGC TCAGCCTGAG GAGCTGCTTC TAACAAATGG AGGTGGACCA
    CCTGGCCTAC AAGAAAATGA TGCACCTGAT TCACAGGTTG AAGCTCATGT TAGCCCAATC
    ACTGAGCCTG AGGACCAACC TGTGACAGTC TCTGATAACG TGCTTGTGGA AGAGGATGTA
    GAAAAAACAT TGCCTGCGGA TACGGAAGTC GTGGCTTCAA CCCAAGTTGT AAAGCAGAGC
    TCCGAGAGTC AGTCTCAGGT TGCTGTTGCT GCACCCAAGA AAAAGCGAAA AATGAAGGAA
    CTAAACAAGA AAGAGGCAGT GGGAGATCTC CTTGATGCGT TCAAAGAGGC TGGTGATGGC
    TCTTCTGATG TGGATTCTTC CTCTAAGCCT ACCAGTGCTG CACCTGAACC AGATCAGAGC
    CCTTGTGCTC CTCCTGAGGA ACAGGATGAA ACTTGGGAAG AGAAGGAAGA TAAACTGGAT
    GCAGAGAATA TTAAACCAGG AGAACATAAA TATAAATACA AAGAAGAGCA ATGGAAACCC
    CTAAACCCGG AGGAAAAGAA GAGTTATGAT CGCGAGTTTC TGCTAGGGTT CCAGTTTATT
    GTGGCCAGTA TGCAGAAACC TGCGGGTCTT CCTCAAATTA CTGATGTGGT GCTGGATAAG
    GCAAACAAAA CTCCATTGCG ACCATTGGAT CCTACACGTC TGACGGGAAT GAACTGTGGG
    CCAGATTTTA CACCTTCATT TGCCAACCTT GGTCGGCCTG CTGTGGGTAA CCGTGGACCA
    CCAGCTGGCA TGGGTCCGAG ACGGTCCCAG CAGGGTCCAC GAAAAGAGCC TCGTAAAATT
    ATTCAAACCG TATCATTAAA TGAGAATGTA CAACTAAACA AAGCTGAAAA GGCATGGAAA
    CCGATAAACA AGAGAGCTGG CGATGAGGAG GACCCAGAAA ATATTAAGAC ACAGGACCTA
    TTCCGAAGGG TTCGCAGTAT CTTGAATAAG CTGACCCCTC AGATGTTCCA GCAGCTGATG
    AAGCAGGTCA CTGACCTTAC TATTGATACC GAAGATCGTC TTAAGGGTGT CATTGACCTA
    ATTTTTGAAA AAGCCATTTC AGAACCCAAT TTCTCTGTTG CATATGCCAA CATGTGTCGT
    TGCCTTATGG GGCTTAAAGT TCCAACCACA GACAAGCCAG GGGTTACAGT CAACTTCCGC
    AAGTTGTTAC TGAATCGGTG CCAGAAGGAG TTTGAGAAGG ATAAGGATGA TGATGAAGTG
    TTTGAGAAGA AACAAAAAGA AATGGATGCT GCTGCTTCGC CGGAGGAAAA AGCACGTTTG
    AATGATGAAC TGGTCGAGGC CCGTGACAGT GCACGACGGC GATCCTTGGG GAACATTAAG
    TTCATTGGAG AACTGTTTAA GTTAAAAATG CTGACTGAAG CTATAATGCA CGATTGTGTG
    GTGAAGCTAT TAAAAAATCA TGATGAGGAA TCTCTGGAGT GCCTCTGCCG ACTGCTGTCC
    ACCATTGGCA AGGACCTGGA TTTTGAGAGA GCTAAGCCAC GTATGGACCA GTACTTCAAC
    CAGATGGATA AAATCATTAA GGAGCGGAAG ACATCTTCAC GAATTAGATT CATGCTGCAG
    GATGTGATTG ATTTGCGTCT GTGTAATTGG GTGCCTCGTC GTGCAGATCA GGGCCCTAAA
    ACTATAGATC AGATTCATAA AGAAGCAGAA ATGGAAAAGC AGAGGGAGCA GGTTAAGGTG
    CAACAACTGA TGTCTAAACA AGACAAGCGG CGAGAGCCCC CAGGACGACC CACTGGAAGC
    CGGGGTAACC AGCCTGCAGA TGATGGCTGG AATACAGTGC CCATCAGTAA GGGCAGCCGT
    CCTATTGACA CCACTAGGAT CACAAAGATA ACAAAGCTTG GTGCTATTGA TTCAAATCAA
    CTGCTTGCCC CAGGAGGACG TCTGAGTTGG GGTAAAGGAA GCAGCGGAGG AACTGGAGCC
    AAACCAGCAG AAACAGCTCC AGAATCTAGT CGGCCTGCGA CCAGCACCTT AAACAGGTTT
    TCTGCCTTGC AGCAGTCTTC ATCTGCTGAG AGTCAAGATG CTAGACGGCC CTTGCATCGG
    GGAAGTTCTA GTCGGGAACG TTCTGAAAAA GATCGTGGAG ACAGATTTGA TAGAGGTGAA
    AGGTTTGATA AGGGAGAACT GTTTGATAGA TCTGACCGTT CAGATCGAGC TCGTCCTCTT
    CTTACAAAGA GAAGCTATAG CAAGGAAACT GAGGACCGTA GCCGAGAAAG AGAGGCCCAT
    ATACCACAGG AGGCTGTGAG GAAGGTGGCA AGTATGACTG AGCAGCGGGA CCGCAGCAAA
    GAAAGTGTGA AAAAAGAAAT TCCAGCACCG ACAGCAGTGG TTGCCTCATC AAAGCCCTCT
    ATGTCTGAGG ATGAGCTAGA GAAGAAATCT AAAGCCATCA TAGAAGAATA TTTGCACATT
    AATGATATGA AGGAAGCTCT TCAGTGTGTA GTGGAGCTTA ATAGTTCAAC GCTGCTTTTT
    ATCTTTGTGC GGAATGGGAT TGAGTCAACA CTAGAGAGAA GCACTATTGC CAGAGAGCAT
    ATGGGTCTCC TTTTGTACCA GTTAGTAAAG GCAGGAACTC TGTCCAAGGA GCAGTATTAC
    AAAGGGGTGC TTGAAGTTCT TGAAGTAGCA GAAGATATGG AAATAGATAT ACCCCACATC
    TGGCTGTATT TAGCAGAGAT AATCAGCCCT GTGCTGCTAG AGGGGGGGAT TCCGATGGGG
    GAGCTGTTCA GAGAGCTGAC CAAACCTTTA GTGCTCATTG GCAAAGCTGG AGTCCTTCTG
    GCAGAAATCC TTGGATTATT GTGCAAAGGC ATGAGTCATA AGAAGGCTGG AGCCCTGTGG
    AAAGAATCTG GGCTAAGCTG GAAGGAATTC TTGTCCAAGG ACCAGGATAT TAACAAGTTT
    ATAACTGACA AGAAACTCGA ATACACAACA GGAGAGGAGA CTGAAACACC CAGTAAGAAA
    GACCTCACCC CTGCTGAACT TGATAAAATT TTGACAAAAC TTTTGAAAGA GAACAGCAGT
    AACCAGAAGA TATTTGACTG GATTGAAGCC AACTTAAATG ACCAGCAAAC TTCATCCAAC
    ACCTTTGTTC GAACTTTAAT GAGTACTGTG TGCCACTCTG CTATTATTTT TGAGAATCCT
    TGTAGGGTTG ATGTTAATAT TATCCATACT AGAGCCAAAT TTCTTCAGAA ATATTTAAGT
    GACGAGGCCA AGGAACTACA GGCTCTCTAT GCTCTACAGG CACTTGTTGT AAAGCTTGAC
    CAGCCCCCAA ATCTCCTACG GATGTTTTTT GATGCTCTCT ATGATGAGGA TGTGATAAAA
    GAGGATCCAT TCTACAAGTG GGAGTCCAGC AAAGACCCAG CTGAACAGCA AGGAAAAGGT
    GTTGCTCTTA AATCTGTCAC TGCCTTCTTC ACATGGCTAC GGGAAGCAGA GGATGAGTCT
    GATAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012818832.1
    CDS341..5149
    Translation

    Target ORF information:

    RefSeq Version XM_012963378.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012963378.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
    CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA
    CAAATGAATA CACAATCTCA GCCTCGACAG CATTATTATC AAAATAGGAC ACAGCCTCCC
    ACTAGTGCCA CCAGAGTGCA GAGTGGTTCT TCTGCTCGAG CAGTTACTCC AGCCCATGTG
    TATCAACCAG GCTCCCAAGT TATGATGATT CCTTCACAGA TTTCATACCC TTCCCAGGGA
    TACTACATTC AGGGACAGGG ACGCTCATAT GTGGTGCCCA CAAACCAGTA CCCTGTGCAG
    ACTGGGGCTC CAGGTTTCTT CCCCAGTGCA AGTCCCTCAG AATATGGCAC ATATGCTGGA
    GCATATTACC CTGCACAACA GACGTTCCCT CCTGGAGTGC CTGGAGCACA AGTTATGATG
    AATCAGCCAC AGGTGATCCC TCCTAAACGG GAGCGCAAAA CGATAAGAAT TAGAGACCCC
    AACCAAGGAG GCAAAGATAT CACAGAAGAA ATTCTCTCAG GAGGAAGAAC ATCCTCTACG
    CCAACGCCTC CACAGTCAAC TGTGGGAAGT TCTGAAATTC AAGCTAATGG AGAAAGCGTT
    CATCATGTTA CCGTTGTTGT GCGCCCAGAT GATGGACCCA AGGCCACCCA AGTGCTGATC
    AAAGCCTCAG AGAGCCCTTC CCCTCCTCTG CCTCTCTCTG AAGCTGAGCC AAGCATAGCA
    AATGTGTGTG ATATTTCAAA AGATAACACT ATCTCTGAGT TGACTTCTCA TATAGTAGAG
    CCCCTAAGTT CAGAAGAGAC AACCATTGAT ACTTCTACCT CTCAGGTGGA TCTCAAACAC
    CAGCCAGTGG CTGCTATGGC AGAGCATGAA ACTCTTATAG AGGAGGCATC AATACAGGAG
    GTGGCCTTTG CAGAGGCACC CATCGCTCAA TCAGAGATAC CAAAATCTCT TGCCTCCCAG
    CCTCTAGCAG AAATCTCTAC CCCTGAACAG ATTTCTAAAC TTGACGCACA AGTGCCATCT
    TTGCAACAAG AGGAGTTACT TACAGAAACT GTTGAACTTA ATTTAAGCAA TCAGGCTCTT
    CCTTCACAAA TTTCTGAGCC TGAACCTTTT GAACCTGAGG CTTCAGCTTC AGATCCTCTA
    CATGAGGAGC CCACAGAGCC CACAGTTTCA ACAGAAGAAC CACAAGCTAA TGGACTGTCT
    GAGGAGACAC CAGTATTACC TGTACAGCCA CAACCAACAT CTGTTTGCCA GACTGAGCCC
    TCCCTAGAAT CCCCCATCGC TCAGCCTGAG GAGCTGCTTC TAACAAATGG AGGTGGACCA
    CCTGGCCTAC AAGAAAATGA TGCACCTGAT TCACAGGTTG AAGCTCATGT TAGCCCAATC
    ACTGAGCCTG AGGACCAACC TGTGACAGTC TCTGATAACG TGCTTGTGGA AGAGGATGTA
    GAAAAAACAT TGCCTGCGGA TACGGAAGTC GTGGCTTCAA CCCAAGTTGT AAAGCAGAGC
    TCCGAGAGTC AGTCTCAGGT TGCTGTTGCT GCACCCAAGA AAAAGCGAAA AATGAAGGAA
    CTAAACAAGA AAGAGGCAGT GGGAGATCTC CTTGATGCGT TCAAAGAGGC TGGTGATGGC
    TCTTCTGATG TGGATTCTTC CTCTAAGCCT ACCAGTGCTG CACCTGAACC AGATCAGAGC
    CCTTGTGCTC CTCCTGAGGA ACAGGATGAA ACTTGGGAAG AGAAGGAAGA TAAACTGGAT
    GCAGAGAATA TTAAACCAGG AGAACATAAA TATAAATACA AAGAAGAGCA ATGGAAACCC
    CTAAACCCGG AGGAAAAGAA GAGTTATGAT CGCGAGTTTC TGCTAGGGTT CCAGTTTATT
    GTGGCCAGTA TGCAGAAACC TGCGGGTCTT CCTCAAATTA CTGATGTGGT GCTGGATAAG
    GCAAACAAAA CTCCATTGCG ACCATTGGAT CCTACACGTC TGACGGGAAT GAACTGTGGG
    CCAGATTTTA CACCTTCATT TGCCAACCTT GGTCGGCCTG CTGTGGGTAA CCGTGGACCA
    CCAGCTGGCA TGGGTCCGAG ACGGTCCCAG CAGGGTCCAC GAAAAGAGCC TCGTAAAATT
    ATTCAAACCG TATCATTAAA TGAGAATGTA CAACTAAACA AAGCTGAAAA GGCATGGAAA
    CCGATAAACA AGAGAGCTGG CGATGAGGAG GACCCAGAAA ATATTAAGAC ACAGGACCTA
    TTCCGAAGGG TTCGCAGTAT CTTGAATAAG CTGACCCCTC AGATGTTCCA GCAGCTGATG
    AAGCAGGTCA CTGACCTTAC TATTGATACC GAAGATCGTC TTAAGGGTGT CATTGACCTA
    ATTTTTGAAA AAGCCATTTC AGAACCCAAT TTCTCTGTTG CATATGCCAA CATGTGTCGT
    TGCCTTATGG GGCTTAAAGT TCCAACCACA GACAAGCCAG GGGTTACAGT CAACTTCCGC
    AAGTTGTTAC TGAATCGGTG CCAGAAGGAG TTTGAGAAGG ATAAGGATGA TGATGAAGTG
    TTTGAGAAGA AACAAAAAGA AATGGATGCT GCTGCTTCGC CGGAGGAAAA AGCACGTTTG
    AATGATGAAC TGGTCGAGGC CCGTGACAGT GCACGACGGC GATCCTTGGG GAACATTAAG
    TTCATTGGAG AACTGTTTAA GTTAAAAATG CTGACTGAAG CTATAATGCA CGATTGTGTG
    GTGAAGCTAT TAAAAAATCA TGATGAGGAA TCTCTGGAGT GCCTCTGCCG ACTGCTGTCC
    ACCATTGGCA AGGACCTGGA TTTTGAGAGA GCTAAGCCAC GTATGGACCA GTACTTCAAC
    CAGATGGATA AAATCATTAA GGAGCGGAAG ACATCTTCAC GAATTAGATT CATGCTGCAG
    GATGTGATTG ATTTGCGTCT GTGTAATTGG GTGCCTCGTC GTGCAGATCA GGGCCCTAAA
    ACTATAGATC AGATTCATAA AGAAGCAGAA ATGGAAAAGC AGAGGGAGCA GGTTAAGGTG
    CAACAACTGA TGTCTAAACA AGACAAGCGG CGAGAGCCCC CAGGACGACC CACTGGAAGC
    CGGGGTAACC AGCCTGCAGA TGATGGCTGG AATACAGTGC CCATCAGTAA GGGCAGCCGT
    CCTATTGACA CCACTAGGAT CACAAAGATA ACAAAGCTTG GTGCTATTGA TTCAAATCAA
    CTGCTTGCCC CAGGAGGACG TCTGAGTTGG GGTAAAGGAA GCAGCGGAGG AACTGGAGCC
    AAACCAGCAG AAACAGCTCC AGAATCTAGT CGGCCTGCGA CCAGCACCTT AAACAGGTTT
    TCTGCCTTGC AGCAGTCTTC ATCTGCTGAG AGTCAAGATG CTAGACGGCC CTTGCATCGG
    GGAAGTTCTA GTCGGGAACG TTCTGAAAAA GATCGTGGAG ACAGATTTGA TAGAGGTGAA
    AGGTTTGATA AGGGAGAACT GTTTGATAGA TCTGACCGTT CAGATCGAGC TCGTCCTCTT
    CTTACAAAGA GAAGCTATAG CAAGGAAACT GAGGACCGTA GCCGAGAAAG AGAGGCCCAT
    ATACCACAGG AGGCTGTGAG GAAGGTGGCA AGTATGACTG AGCAGCGGGA CCGCAGCAAA
    GAAAGTGTGA AAAAAGAAAT TCCAGCACCG ACAGCAGTGG TTGCCTCATC AAAGCCCTCT
    ATGTCTGAGG ATGAGCTAGA GAAGAAATCT AAAGCCATCA TAGAAGAATA TTTGCACATT
    AATGATATGA AGGAAGCTCT TCAGTGTGTA GTGGAGCTTA ATAGTTCAAC GCTGCTTTTT
    ATCTTTGTGC GGAATGGGAT TGAGTCAACA CTAGAGAGAA GCACTATTGC CAGAGAGCAT
    ATGGGTCTCC TTTTGTACCA GTTAGTAAAG GCAGGAACTC TGTCCAAGGA GCAGTATTAC
    AAAGGGGTGC TTGAAGTTCT TGAAGTAGCA GAAGATATGG AAATAGATAT ACCCCACATC
    TGGCTGTATT TAGCAGAGAT AATCAGCCCT GTGCTGCTAG AGGGGGGGAT TCCGATGGGG
    GAGCTGTTCA GAGAGCTGAC CAAACCTTTA GTGCTCATTG GCAAAGCTGG AGTCCTTCTG
    GCAGAAATCC TTGGATTATT GTGCAAAGGC ATGAGTCATA AGAAGGCTGG AGCCCTGTGG
    AAAGAATCTG GGCTAAGCTG GAAGGAATTC TTGTCCAAGG ACCAGGATAT TAACAAGTTT
    ATAACTGACA AGAAACTCGA ATACACAACA GGAGAGGAGA CTGAAACACC CAGTAAGAAA
    GACCTCACCC CTGCTGAACT TGATAAAATT TTGACAAAAC TTTTGAAAGA GAACAGCAGT
    AACCAGAAGA TATTTGACTG GATTGAAGCC AACTTAAATG ACCAGCAAAC TTCATCCAAC
    ACCTTTGTTC GAACTTTAAT GAGTACTGTG TGCCACTCTG CTATTATTTT TGAGAATCCT
    TGTAGGGTTG ATGTTAATAT TATCCATACT AGAGCCAAAT TTCTTCAGAA ATATTTAAGT
    GACGAGGCCA AGGAACTACA GGCTCTCTAT GCTCTACAGG CACTTGTTGT AAAGCTTGAC
    CAGCCCCCAA ATCTCCTACG GATGTTTTTT GATGCTCTCT ATGATGAGGA TGTGATAAAA
    GAGGATCCAT TCTACAAGTG GGAGTCCAGC AAAGACCCAG CTGAACAGCA AGGAAAAGGT
    GTTGCTCTTA AATCTGTCAC TGCCTTCTTC ACATGGCTAC GGGAAGCAGA GGATGAGTCT
    GATAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa10205
    Clone ID Related Accession (Same CDS sequence) XM_004914401.4 , XM_004914400.3 , XM_004914401.3 , XM_012963378.2 , XM_012963378.3 , XM_004914400.4
    Accession Version XM_004914400.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4809bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004914400.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
    CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA
    CAAATGAATA CACAATCTCA GCCTCGACAG CATTATTATC AAAATAGGAC ACAGCCTCCC
    ACTAGTGCCA CCAGAGTGCA GAGTGGTTCT TCTGCTCGAG CAGTTACTCC AGCCCATGTG
    TATCAACCAG GCTCCCAAGT TATGATGATT CCTTCACAGA TTTCATACCC TTCCCAGGGA
    TACTACATTC AGGGACAGGG ACGCTCATAT GTGGTGCCCA CAAACCAGTA CCCTGTGCAG
    ACTGGGGCTC CAGGTTTCTT CCCCAGTGCA AGTCCCTCAG AATATGGCAC ATATGCTGGA
    GCATATTACC CTGCACAACA GACGTTCCCT CCTGGAGTGC CTGGAGCACA AGTTATGATG
    AATCAGCCAC AGGTGATCCC TCCTAAACGG GAGCGCAAAA CGATAAGAAT TAGAGACCCC
    AACCAAGGAG GCAAAGATAT CACAGAAGAA ATTCTCTCAG GAGGAAGAAC ATCCTCTACG
    CCAACGCCTC CACAGTCAAC TGTGGGAAGT TCTGAAATTC AAGCTAATGG AGAAAGCGTT
    CATCATGTTA CCGTTGTTGT GCGCCCAGAT GATGGACCCA AGGCCACCCA AGTGCTGATC
    AAAGCCTCAG AGAGCCCTTC CCCTCCTCTG CCTCTCTCTG AAGCTGAGCC AAGCATAGCA
    AATGTGTGTG ATATTTCAAA AGATAACACT ATCTCTGAGT TGACTTCTCA TATAGTAGAG
    CCCCTAAGTT CAGAAGAGAC AACCATTGAT ACTTCTACCT CTCAGGTGGA TCTCAAACAC
    CAGCCAGTGG CTGCTATGGC AGAGCATGAA ACTCTTATAG AGGAGGCATC AATACAGGAG
    GTGGCCTTTG CAGAGGCACC CATCGCTCAA TCAGAGATAC CAAAATCTCT TGCCTCCCAG
    CCTCTAGCAG AAATCTCTAC CCCTGAACAG ATTTCTAAAC TTGACGCACA AGTGCCATCT
    TTGCAACAAG AGGAGTTACT TACAGAAACT GTTGAACTTA ATTTAAGCAA TCAGGCTCTT
    CCTTCACAAA TTTCTGAGCC TGAACCTTTT GAACCTGAGG CTTCAGCTTC AGATCCTCTA
    CATGAGGAGC CCACAGAGCC CACAGTTTCA ACAGAAGAAC CACAAGCTAA TGGACTGTCT
    GAGGAGACAC CAGTATTACC TGTACAGCCA CAACCAACAT CTGTTTGCCA GACTGAGCCC
    TCCCTAGAAT CCCCCATCGC TCAGCCTGAG GAGCTGCTTC TAACAAATGG AGGTGGACCA
    CCTGGCCTAC AAGAAAATGA TGCACCTGAT TCACAGGTTG AAGCTCATGT TAGCCCAATC
    ACTGAGCCTG AGGACCAACC TGTGACAGTC TCTGATAACG TGCTTGTGGA AGAGGATGTA
    GAAAAAACAT TGCCTGCGGA TACGGAAGTC GTGGCTTCAA CCCAAGTTGT AAAGCAGAGC
    TCCGAGAGTC AGTCTCAGGT TGCTGTTGCT GCACCCAAGA AAAAGCGAAA AATGAAGGAA
    CTAAACAAGA AAGAGGCAGT GGGAGATCTC CTTGATGCGT TCAAAGAGGC TGGTGATGGC
    TCTTCTGATG TGGATTCTTC CTCTAAGCCT ACCAGTGCTG CACCTGAACC AGATCAGAGC
    CCTTGTGCTC CTCCTGAGGA ACAGGATGAA ACTTGGGAAG AGAAGGAAGA TAAACTGGAT
    GCAGAGAATA TTAAACCAGG AGAACATAAA TATAAATACA AAGAAGAGCA ATGGAAACCC
    CTAAACCCGG AGGAAAAGAA GAGTTATGAT CGCGAGTTTC TGCTAGGGTT CCAGTTTATT
    GTGGCCAGTA TGCAGAAACC TGCGGGTCTT CCTCAAATTA CTGATGTGGT GCTGGATAAG
    GCAAACAAAA CTCCATTGCG ACCATTGGAT CCTACACGTC TGACGGGAAT GAACTGTGGG
    CCAGATTTTA CACCTTCATT TGCCAACCTT GGTCGGCCTG CTGTGGGTAA CCGTGGACCA
    CCAGCTGGCA TGGGTCCGAG ACGGTCCCAG CAGGGTCCAC GAAAAGAGCC TCGTAAAATT
    ATTCAAACCG TATCATTAAA TGAGAATGTA CAACTAAACA AAGCTGAAAA GGCATGGAAA
    CCGATAAACA AGAGAGCTGG CGATGAGGAG GACCCAGAAA ATATTAAGAC ACAGGACCTA
    TTCCGAAGGG TTCGCAGTAT CTTGAATAAG CTGACCCCTC AGATGTTCCA GCAGCTGATG
    AAGCAGGTCA CTGACCTTAC TATTGATACC GAAGATCGTC TTAAGGGTGT CATTGACCTA
    ATTTTTGAAA AAGCCATTTC AGAACCCAAT TTCTCTGTTG CATATGCCAA CATGTGTCGT
    TGCCTTATGG GGCTTAAAGT TCCAACCACA GACAAGCCAG GGGTTACAGT CAACTTCCGC
    AAGTTGTTAC TGAATCGGTG CCAGAAGGAG TTTGAGAAGG ATAAGGATGA TGATGAAGTG
    TTTGAGAAGA AACAAAAAGA AATGGATGCT GCTGCTTCGC CGGAGGAAAA AGCACGTTTG
    AATGATGAAC TGGTCGAGGC CCGTGACAGT GCACGACGGC GATCCTTGGG GAACATTAAG
    TTCATTGGAG AACTGTTTAA GTTAAAAATG CTGACTGAAG CTATAATGCA CGATTGTGTG
    GTGAAGCTAT TAAAAAATCA TGATGAGGAA TCTCTGGAGT GCCTCTGCCG ACTGCTGTCC
    ACCATTGGCA AGGACCTGGA TTTTGAGAGA GCTAAGCCAC GTATGGACCA GTACTTCAAC
    CAGATGGATA AAATCATTAA GGAGCGGAAG ACATCTTCAC GAATTAGATT CATGCTGCAG
    GATGTGATTG ATTTGCGTCT GTGTAATTGG GTGCCTCGTC GTGCAGATCA GGGCCCTAAA
    ACTATAGATC AGATTCATAA AGAAGCAGAA ATGGAAAAGC AGAGGGAGCA GGTTAAGGTG
    CAACAACTGA TGTCTAAACA AGACAAGCGG CGAGAGCCCC CAGGACGACC CACTGGAAGC
    CGGGGTAACC AGCCTGCAGA TGATGGCTGG AATACAGTGC CCATCAGTAA GGGCAGCCGT
    CCTATTGACA CCACTAGGAT CACAAAGATA ACAAAGCTTG GTGCTATTGA TTCAAATCAA
    CTGCTTGCCC CAGGAGGACG TCTGAGTTGG GGTAAAGGAA GCAGCGGAGG AACTGGAGCC
    AAACCAGCAG AAACAGCTCC AGAATCTAGT CGGCCTGCGA CCAGCACCTT AAACAGGTTT
    TCTGCCTTGC AGCAGTCTTC ATCTGCTGAG AGTCAAGATG CTAGACGGCC CTTGCATCGG
    GGAAGTTCTA GTCGGGAACG TTCTGAAAAA GATCGTGGAG ACAGATTTGA TAGAGGTGAA
    AGGTTTGATA AGGGAGAACT GTTTGATAGA TCTGACCGTT CAGATCGAGC TCGTCCTCTT
    CTTACAAAGA GAAGCTATAG CAAGGAAACT GAGGACCGTA GCCGAGAAAG AGAGGCCCAT
    ATACCACAGG AGGCTGTGAG GAAGGTGGCA AGTATGACTG AGCAGCGGGA CCGCAGCAAA
    GAAAGTGTGA AAAAAGAAAT TCCAGCACCG ACAGCAGTGG TTGCCTCATC AAAGCCCTCT
    ATGTCTGAGG ATGAGCTAGA GAAGAAATCT AAAGCCATCA TAGAAGAATA TTTGCACATT
    AATGATATGA AGGAAGCTCT TCAGTGTGTA GTGGAGCTTA ATAGTTCAAC GCTGCTTTTT
    ATCTTTGTGC GGAATGGGAT TGAGTCAACA CTAGAGAGAA GCACTATTGC CAGAGAGCAT
    ATGGGTCTCC TTTTGTACCA GTTAGTAAAG GCAGGAACTC TGTCCAAGGA GCAGTATTAC
    AAAGGGGTGC TTGAAGTTCT TGAAGTAGCA GAAGATATGG AAATAGATAT ACCCCACATC
    TGGCTGTATT TAGCAGAGAT AATCAGCCCT GTGCTGCTAG AGGGGGGGAT TCCGATGGGG
    GAGCTGTTCA GAGAGCTGAC CAAACCTTTA GTGCTCATTG GCAAAGCTGG AGTCCTTCTG
    GCAGAAATCC TTGGATTATT GTGCAAAGGC ATGAGTCATA AGAAGGCTGG AGCCCTGTGG
    AAAGAATCTG GGCTAAGCTG GAAGGAATTC TTGTCCAAGG ACCAGGATAT TAACAAGTTT
    ATAACTGACA AGAAACTCGA ATACACAACA GGAGAGGAGA CTGAAACACC CAGTAAGAAA
    GACCTCACCC CTGCTGAACT TGATAAAATT TTGACAAAAC TTTTGAAAGA GAACAGCAGT
    AACCAGAAGA TATTTGACTG GATTGAAGCC AACTTAAATG ACCAGCAAAC TTCATCCAAC
    ACCTTTGTTC GAACTTTAAT GAGTACTGTG TGCCACTCTG CTATTATTTT TGAGAATCCT
    TGTAGGGTTG ATGTTAATAT TATCCATACT AGAGCCAAAT TTCTTCAGAA ATATTTAAGT
    GACGAGGCCA AGGAACTACA GGCTCTCTAT GCTCTACAGG CACTTGTTGT AAAGCTTGAC
    CAGCCCCCAA ATCTCCTACG GATGTTTTTT GATGCTCTCT ATGATGAGGA TGTGATAAAA
    GAGGATCCAT TCTACAAGTG GGAGTCCAGC AAAGACCCAG CTGAACAGCA AGGAAAAGGT
    GTTGCTCTTA AATCTGTCAC TGCCTTCTTC ACATGGCTAC GGGAAGCAGA GGATGAGTCT
    GATAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004914457.1
    CDS213..5021
    Translation

    Target ORF information:

    RefSeq Version XM_004914400.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004914400.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
    CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA
    CAAATGAATA CACAATCTCA GCCTCGACAG CATTATTATC AAAATAGGAC ACAGCCTCCC
    ACTAGTGCCA CCAGAGTGCA GAGTGGTTCT TCTGCTCGAG CAGTTACTCC AGCCCATGTG
    TATCAACCAG GCTCCCAAGT TATGATGATT CCTTCACAGA TTTCATACCC TTCCCAGGGA
    TACTACATTC AGGGACAGGG ACGCTCATAT GTGGTGCCCA CAAACCAGTA CCCTGTGCAG
    ACTGGGGCTC CAGGTTTCTT CCCCAGTGCA AGTCCCTCAG AATATGGCAC ATATGCTGGA
    GCATATTACC CTGCACAACA GACGTTCCCT CCTGGAGTGC CTGGAGCACA AGTTATGATG
    AATCAGCCAC AGGTGATCCC TCCTAAACGG GAGCGCAAAA CGATAAGAAT TAGAGACCCC
    AACCAAGGAG GCAAAGATAT CACAGAAGAA ATTCTCTCAG GAGGAAGAAC ATCCTCTACG
    CCAACGCCTC CACAGTCAAC TGTGGGAAGT TCTGAAATTC AAGCTAATGG AGAAAGCGTT
    CATCATGTTA CCGTTGTTGT GCGCCCAGAT GATGGACCCA AGGCCACCCA AGTGCTGATC
    AAAGCCTCAG AGAGCCCTTC CCCTCCTCTG CCTCTCTCTG AAGCTGAGCC AAGCATAGCA
    AATGTGTGTG ATATTTCAAA AGATAACACT ATCTCTGAGT TGACTTCTCA TATAGTAGAG
    CCCCTAAGTT CAGAAGAGAC AACCATTGAT ACTTCTACCT CTCAGGTGGA TCTCAAACAC
    CAGCCAGTGG CTGCTATGGC AGAGCATGAA ACTCTTATAG AGGAGGCATC AATACAGGAG
    GTGGCCTTTG CAGAGGCACC CATCGCTCAA TCAGAGATAC CAAAATCTCT TGCCTCCCAG
    CCTCTAGCAG AAATCTCTAC CCCTGAACAG ATTTCTAAAC TTGACGCACA AGTGCCATCT
    TTGCAACAAG AGGAGTTACT TACAGAAACT GTTGAACTTA ATTTAAGCAA TCAGGCTCTT
    CCTTCACAAA TTTCTGAGCC TGAACCTTTT GAACCTGAGG CTTCAGCTTC AGATCCTCTA
    CATGAGGAGC CCACAGAGCC CACAGTTTCA ACAGAAGAAC CACAAGCTAA TGGACTGTCT
    GAGGAGACAC CAGTATTACC TGTACAGCCA CAACCAACAT CTGTTTGCCA GACTGAGCCC
    TCCCTAGAAT CCCCCATCGC TCAGCCTGAG GAGCTGCTTC TAACAAATGG AGGTGGACCA
    CCTGGCCTAC AAGAAAATGA TGCACCTGAT TCACAGGTTG AAGCTCATGT TAGCCCAATC
    ACTGAGCCTG AGGACCAACC TGTGACAGTC TCTGATAACG TGCTTGTGGA AGAGGATGTA
    GAAAAAACAT TGCCTGCGGA TACGGAAGTC GTGGCTTCAA CCCAAGTTGT AAAGCAGAGC
    TCCGAGAGTC AGTCTCAGGT TGCTGTTGCT GCACCCAAGA AAAAGCGAAA AATGAAGGAA
    CTAAACAAGA AAGAGGCAGT GGGAGATCTC CTTGATGCGT TCAAAGAGGC TGGTGATGGC
    TCTTCTGATG TGGATTCTTC CTCTAAGCCT ACCAGTGCTG CACCTGAACC AGATCAGAGC
    CCTTGTGCTC CTCCTGAGGA ACAGGATGAA ACTTGGGAAG AGAAGGAAGA TAAACTGGAT
    GCAGAGAATA TTAAACCAGG AGAACATAAA TATAAATACA AAGAAGAGCA ATGGAAACCC
    CTAAACCCGG AGGAAAAGAA GAGTTATGAT CGCGAGTTTC TGCTAGGGTT CCAGTTTATT
    GTGGCCAGTA TGCAGAAACC TGCGGGTCTT CCTCAAATTA CTGATGTGGT GCTGGATAAG
    GCAAACAAAA CTCCATTGCG ACCATTGGAT CCTACACGTC TGACGGGAAT GAACTGTGGG
    CCAGATTTTA CACCTTCATT TGCCAACCTT GGTCGGCCTG CTGTGGGTAA CCGTGGACCA
    CCAGCTGGCA TGGGTCCGAG ACGGTCCCAG CAGGGTCCAC GAAAAGAGCC TCGTAAAATT
    ATTCAAACCG TATCATTAAA TGAGAATGTA CAACTAAACA AAGCTGAAAA GGCATGGAAA
    CCGATAAACA AGAGAGCTGG CGATGAGGAG GACCCAGAAA ATATTAAGAC ACAGGACCTA
    TTCCGAAGGG TTCGCAGTAT CTTGAATAAG CTGACCCCTC AGATGTTCCA GCAGCTGATG
    AAGCAGGTCA CTGACCTTAC TATTGATACC GAAGATCGTC TTAAGGGTGT CATTGACCTA
    ATTTTTGAAA AAGCCATTTC AGAACCCAAT TTCTCTGTTG CATATGCCAA CATGTGTCGT
    TGCCTTATGG GGCTTAAAGT TCCAACCACA GACAAGCCAG GGGTTACAGT CAACTTCCGC
    AAGTTGTTAC TGAATCGGTG CCAGAAGGAG TTTGAGAAGG ATAAGGATGA TGATGAAGTG
    TTTGAGAAGA AACAAAAAGA AATGGATGCT GCTGCTTCGC CGGAGGAAAA AGCACGTTTG
    AATGATGAAC TGGTCGAGGC CCGTGACAGT GCACGACGGC GATCCTTGGG GAACATTAAG
    TTCATTGGAG AACTGTTTAA GTTAAAAATG CTGACTGAAG CTATAATGCA CGATTGTGTG
    GTGAAGCTAT TAAAAAATCA TGATGAGGAA TCTCTGGAGT GCCTCTGCCG ACTGCTGTCC
    ACCATTGGCA AGGACCTGGA TTTTGAGAGA GCTAAGCCAC GTATGGACCA GTACTTCAAC
    CAGATGGATA AAATCATTAA GGAGCGGAAG ACATCTTCAC GAATTAGATT CATGCTGCAG
    GATGTGATTG ATTTGCGTCT GTGTAATTGG GTGCCTCGTC GTGCAGATCA GGGCCCTAAA
    ACTATAGATC AGATTCATAA AGAAGCAGAA ATGGAAAAGC AGAGGGAGCA GGTTAAGGTG
    CAACAACTGA TGTCTAAACA AGACAAGCGG CGAGAGCCCC CAGGACGACC CACTGGAAGC
    CGGGGTAACC AGCCTGCAGA TGATGGCTGG AATACAGTGC CCATCAGTAA GGGCAGCCGT
    CCTATTGACA CCACTAGGAT CACAAAGATA ACAAAGCTTG GTGCTATTGA TTCAAATCAA
    CTGCTTGCCC CAGGAGGACG TCTGAGTTGG GGTAAAGGAA GCAGCGGAGG AACTGGAGCC
    AAACCAGCAG AAACAGCTCC AGAATCTAGT CGGCCTGCGA CCAGCACCTT AAACAGGTTT
    TCTGCCTTGC AGCAGTCTTC ATCTGCTGAG AGTCAAGATG CTAGACGGCC CTTGCATCGG
    GGAAGTTCTA GTCGGGAACG TTCTGAAAAA GATCGTGGAG ACAGATTTGA TAGAGGTGAA
    AGGTTTGATA AGGGAGAACT GTTTGATAGA TCTGACCGTT CAGATCGAGC TCGTCCTCTT
    CTTACAAAGA GAAGCTATAG CAAGGAAACT GAGGACCGTA GCCGAGAAAG AGAGGCCCAT
    ATACCACAGG AGGCTGTGAG GAAGGTGGCA AGTATGACTG AGCAGCGGGA CCGCAGCAAA
    GAAAGTGTGA AAAAAGAAAT TCCAGCACCG ACAGCAGTGG TTGCCTCATC AAAGCCCTCT
    ATGTCTGAGG ATGAGCTAGA GAAGAAATCT AAAGCCATCA TAGAAGAATA TTTGCACATT
    AATGATATGA AGGAAGCTCT TCAGTGTGTA GTGGAGCTTA ATAGTTCAAC GCTGCTTTTT
    ATCTTTGTGC GGAATGGGAT TGAGTCAACA CTAGAGAGAA GCACTATTGC CAGAGAGCAT
    ATGGGTCTCC TTTTGTACCA GTTAGTAAAG GCAGGAACTC TGTCCAAGGA GCAGTATTAC
    AAAGGGGTGC TTGAAGTTCT TGAAGTAGCA GAAGATATGG AAATAGATAT ACCCCACATC
    TGGCTGTATT TAGCAGAGAT AATCAGCCCT GTGCTGCTAG AGGGGGGGAT TCCGATGGGG
    GAGCTGTTCA GAGAGCTGAC CAAACCTTTA GTGCTCATTG GCAAAGCTGG AGTCCTTCTG
    GCAGAAATCC TTGGATTATT GTGCAAAGGC ATGAGTCATA AGAAGGCTGG AGCCCTGTGG
    AAAGAATCTG GGCTAAGCTG GAAGGAATTC TTGTCCAAGG ACCAGGATAT TAACAAGTTT
    ATAACTGACA AGAAACTCGA ATACACAACA GGAGAGGAGA CTGAAACACC CAGTAAGAAA
    GACCTCACCC CTGCTGAACT TGATAAAATT TTGACAAAAC TTTTGAAAGA GAACAGCAGT
    AACCAGAAGA TATTTGACTG GATTGAAGCC AACTTAAATG ACCAGCAAAC TTCATCCAAC
    ACCTTTGTTC GAACTTTAAT GAGTACTGTG TGCCACTCTG CTATTATTTT TGAGAATCCT
    TGTAGGGTTG ATGTTAATAT TATCCATACT AGAGCCAAAT TTCTTCAGAA ATATTTAAGT
    GACGAGGCCA AGGAACTACA GGCTCTCTAT GCTCTACAGG CACTTGTTGT AAAGCTTGAC
    CAGCCCCCAA ATCTCCTACG GATGTTTTTT GATGCTCTCT ATGATGAGGA TGTGATAAAA
    GAGGATCCAT TCTACAAGTG GGAGTCCAGC AAAGACCCAG CTGAACAGCA AGGAAAAGGT
    GTTGCTCTTA AATCTGTCAC TGCCTTCTTC ACATGGCTAC GGGAAGCAGA GGATGAGTCT
    GATAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa10206
    Clone ID Related Accession (Same CDS sequence) XM_004914402.3 , XM_004914402.4
    Accession Version XM_004914402.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4641bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004914402.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
    CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA
    CAAATGAATA CACAATCTCA GCCTCGACAG GGACGCTCAT ATGTGGTGCC CACAAACCAG
    TACCCTGTGC AGACTGGGGC TCCAGGTTTC TTCCCCAGTG CAAGTCCCTC AGAATATGGC
    ACATATGCTG GAGCATATTA CCCTGCACAA CAGACGTTCC CTCCTGGAGT GCCTGGAGCA
    CAAGTTATGA TGAATCAGCC ACAGGTGATC CCTCCTAAAC GGGAGCGCAA AACGATAAGA
    ATTAGAGACC CCAACCAAGG AGGCAAAGAT ATCACAGAAG AAATTCTCTC AGGAGGAAGA
    ACATCCTCTA CGCCAACGCC TCCACAGTCA ACTGTGGGAA GTTCTGAAAT TCAAGCTAAT
    GGAGAAAGCG TTCATCATGT TACCGTTGTT GTGCGCCCAG ATGATGGACC CAAGGCCACC
    CAAGTGCTGA TCAAAGCCTC AGAGAGCCCT TCCCCTCCTC TGCCTCTCTC TGAAGCTGAG
    CCAAGCATAG CAAATGTGTG TGATATTTCA AAAGATAACA CTATCTCTGA GTTGACTTCT
    CATATAGTAG AGCCCCTAAG TTCAGAAGAG ACAACCATTG ATACTTCTAC CTCTCAGGTG
    GATCTCAAAC ACCAGCCAGT GGCTGCTATG GCAGAGCATG AAACTCTTAT AGAGGAGGCA
    TCAATACAGG AGGTGGCCTT TGCAGAGGCA CCCATCGCTC AATCAGAGAT ACCAAAATCT
    CTTGCCTCCC AGCCTCTAGC AGAAATCTCT ACCCCTGAAC AGATTTCTAA ACTTGACGCA
    CAAGTGCCAT CTTTGCAACA AGAGGAGTTA CTTACAGAAA CTGTTGAACT TAATTTAAGC
    AATCAGGCTC TTCCTTCACA AATTTCTGAG CCTGAACCTT TTGAACCTGA GGCTTCAGCT
    TCAGATCCTC TACATGAGGA GCCCACAGAG CCCACAGTTT CAACAGAAGA ACCACAAGCT
    AATGGACTGT CTGAGGAGAC ACCAGTATTA CCTGTACAGC CACAACCAAC ATCTGTTTGC
    CAGACTGAGC CCTCCCTAGA ATCCCCCATC GCTCAGCCTG AGGAGCTGCT TCTAACAAAT
    GGAGGTGGAC CACCTGGCCT ACAAGAAAAT GATGCACCTG ATTCACAGGT TGAAGCTCAT
    GTTAGCCCAA TCACTGAGCC TGAGGACCAA CCTGTGACAG TCTCTGATAA CGTGCTTGTG
    GAAGAGGATG TAGAAAAAAC ATTGCCTGCG GATACGGAAG TCGTGGCTTC AACCCAAGTT
    GTAAAGCAGA GCTCCGAGAG TCAGTCTCAG GTTGCTGTTG CTGCACCCAA GAAAAAGCGA
    AAAATGAAGG AACTAAACAA GAAAGAGGCA GTGGGAGATC TCCTTGATGC GTTCAAAGAG
    GCTGGTGATG GCTCTTCTGA TGTGGATTCT TCCTCTAAGC CTACCAGTGC TGCACCTGAA
    CCAGATCAGA GCCCTTGTGC TCCTCCTGAG GAACAGGATG AAACTTGGGA AGAGAAGGAA
    GATAAACTGG ATGCAGAGAA TATTAAACCA GGAGAACATA AATATAAATA CAAAGAAGAG
    CAATGGAAAC CCCTAAACCC GGAGGAAAAG AAGAGTTATG ATCGCGAGTT TCTGCTAGGG
    TTCCAGTTTA TTGTGGCCAG TATGCAGAAA CCTGCGGGTC TTCCTCAAAT TACTGATGTG
    GTGCTGGATA AGGCAAACAA AACTCCATTG CGACCATTGG ATCCTACACG TCTGACGGGA
    ATGAACTGTG GGCCAGATTT TACACCTTCA TTTGCCAACC TTGGTCGGCC TGCTGTGGGT
    AACCGTGGAC CACCAGCTGG CATGGGTCCG AGACGGTCCC AGCAGGGTCC ACGAAAAGAG
    CCTCGTAAAA TTATTCAAAC CGTATCATTA AATGAGAATG TACAACTAAA CAAAGCTGAA
    AAGGCATGGA AACCGATAAA CAAGAGAGCT GGCGATGAGG AGGACCCAGA AAATATTAAG
    ACACAGGACC TATTCCGAAG GGTTCGCAGT ATCTTGAATA AGCTGACCCC TCAGATGTTC
    CAGCAGCTGA TGAAGCAGGT CACTGACCTT ACTATTGATA CCGAAGATCG TCTTAAGGGT
    GTCATTGACC TAATTTTTGA AAAAGCCATT TCAGAACCCA ATTTCTCTGT TGCATATGCC
    AACATGTGTC GTTGCCTTAT GGGGCTTAAA GTTCCAACCA CAGACAAGCC AGGGGTTACA
    GTCAACTTCC GCAAGTTGTT ACTGAATCGG TGCCAGAAGG AGTTTGAGAA GGATAAGGAT
    GATGATGAAG TGTTTGAGAA GAAACAAAAA GAAATGGATG CTGCTGCTTC GCCGGAGGAA
    AAAGCACGTT TGAATGATGA ACTGGTCGAG GCCCGTGACA GTGCACGACG GCGATCCTTG
    GGGAACATTA AGTTCATTGG AGAACTGTTT AAGTTAAAAA TGCTGACTGA AGCTATAATG
    CACGATTGTG TGGTGAAGCT ATTAAAAAAT CATGATGAGG AATCTCTGGA GTGCCTCTGC
    CGACTGCTGT CCACCATTGG CAAGGACCTG GATTTTGAGA GAGCTAAGCC ACGTATGGAC
    CAGTACTTCA ACCAGATGGA TAAAATCATT AAGGAGCGGA AGACATCTTC ACGAATTAGA
    TTCATGCTGC AGGATGTGAT TGATTTGCGT CTGTGTAATT GGGTGCCTCG TCGTGCAGAT
    CAGGGCCCTA AAACTATAGA TCAGATTCAT AAAGAAGCAG AAATGGAAAA GCAGAGGGAG
    CAGGTTAAGG TGCAACAACT GATGTCTAAA CAAGACAAGC GGCGAGAGCC CCCAGGACGA
    CCCACTGGAA GCCGGGGTAA CCAGCCTGCA GATGATGGCT GGAATACAGT GCCCATCAGT
    AAGGGCAGCC GTCCTATTGA CACCACTAGG ATCACAAAGA TAACAAAGCT TGGTGCTATT
    GATTCAAATC AACTGCTTGC CCCAGGAGGA CGTCTGAGTT GGGGTAAAGG AAGCAGCGGA
    GGAACTGGAG CCAAACCAGC AGAAACAGCT CCAGAATCTA GTCGGCCTGC GACCAGCACC
    TTAAACAGGT TTTCTGCCTT GCAGCAGTCT TCATCTGCTG AGAGTCAAGA TGCTAGACGG
    CCCTTGCATC GGGGAAGTTC TAGTCGGGAA CGTTCTGAAA AAGATCGTGG AGACAGATTT
    GATAGAGGTG AAAGGTTTGA TAAGGGAGAA CTGTTTGATA GATCTGACCG TTCAGATCGA
    GCTCGTCCTC TTCTTACAAA GAGAAGCTAT AGCAAGGAAA CTGAGGACCG TAGCCGAGAA
    AGAGAGGCCC ATATACCACA GGAGGCTGTG AGGAAGGTGG CAAGTATGAC TGAGCAGCGG
    GACCGCAGCA AAGAAAGTGT GAAAAAAGAA ATTCCAGCAC CGACAGCAGT GGTTGCCTCA
    TCAAAGCCCT CTATGTCTGA GGATGAGCTA GAGAAGAAAT CTAAAGCCAT CATAGAAGAA
    TATTTGCACA TTAATGATAT GAAGGAAGCT CTTCAGTGTG TAGTGGAGCT TAATAGTTCA
    ACGCTGCTTT TTATCTTTGT GCGGAATGGG ATTGAGTCAA CACTAGAGAG AAGCACTATT
    GCCAGAGAGC ATATGGGTCT CCTTTTGTAC CAGTTAGTAA AGGCAGGAAC TCTGTCCAAG
    GAGCAGTATT ACAAAGGGGT GCTTGAAGTT CTTGAAGTAG CAGAAGATAT GGAAATAGAT
    ATACCCCACA TCTGGCTGTA TTTAGCAGAG ATAATCAGCC CTGTGCTGCT AGAGGGGGGG
    ATTCCGATGG GGGAGCTGTT CAGAGAGCTG ACCAAACCTT TAGTGCTCAT TGGCAAAGCT
    GGAGTCCTTC TGGCAGAAAT CCTTGGATTA TTGTGCAAAG GCATGAGTCA TAAGAAGGCT
    GGAGCCCTGT GGAAAGAATC TGGGCTAAGC TGGAAGGAAT TCTTGTCCAA GGACCAGGAT
    ATTAACAAGT TTATAACTGA CAAGAAACTC GAATACACAA CAGGAGAGGA GACTGAAACA
    CCCAGTAAGA AAGACCTCAC CCCTGCTGAA CTTGATAAAA TTTTGACAAA ACTTTTGAAA
    GAGAACAGCA GTAACCAGAA GATATTTGAC TGGATTGAAG CCAACTTAAA TGACCAGCAA
    ACTTCATCCA ACACCTTTGT TCGAACTTTA ATGAGTACTG TGTGCCACTC TGCTATTATT
    TTTGAGAATC CTTGTAGGGT TGATGTTAAT ATTATCCATA CTAGAGCCAA ATTTCTTCAG
    AAATATTTAA GTGACGAGGC CAAGGAACTA CAGGCTCTCT ATGCTCTACA GGCACTTGTT
    GTAAAGCTTG ACCAGCCCCC AAATCTCCTA CGGATGTTTT TTGATGCTCT CTATGATGAG
    GATGTGATAA AAGAGGATCC ATTCTACAAG TGGGAGTCCA GCAAAGACCC AGCTGAACAG
    CAAGGAAAAG GTGTTGCTCT TAAATCTGTC ACTGCCTTCT TCACATGGCT ACGGGAAGCA
    GAGGATGAGT CTGATAATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004914459.1
    CDS214..4854
    Translation

    Target ORF information:

    RefSeq Version XM_004914402.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004914402.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
    CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA
    CAAATGAATA CACAATCTCA GCCTCGACAG GGACGCTCAT ATGTGGTGCC CACAAACCAG
    TACCCTGTGC AGACTGGGGC TCCAGGTTTC TTCCCCAGTG CAAGTCCCTC AGAATATGGC
    ACATATGCTG GAGCATATTA CCCTGCACAA CAGACGTTCC CTCCTGGAGT GCCTGGAGCA
    CAAGTTATGA TGAATCAGCC ACAGGTGATC CCTCCTAAAC GGGAGCGCAA AACGATAAGA
    ATTAGAGACC CCAACCAAGG AGGCAAAGAT ATCACAGAAG AAATTCTCTC AGGAGGAAGA
    ACATCCTCTA CGCCAACGCC TCCACAGTCA ACTGTGGGAA GTTCTGAAAT TCAAGCTAAT
    GGAGAAAGCG TTCATCATGT TACCGTTGTT GTGCGCCCAG ATGATGGACC CAAGGCCACC
    CAAGTGCTGA TCAAAGCCTC AGAGAGCCCT TCCCCTCCTC TGCCTCTCTC TGAAGCTGAG
    CCAAGCATAG CAAATGTGTG TGATATTTCA AAAGATAACA CTATCTCTGA GTTGACTTCT
    CATATAGTAG AGCCCCTAAG TTCAGAAGAG ACAACCATTG ATACTTCTAC CTCTCAGGTG
    GATCTCAAAC ACCAGCCAGT GGCTGCTATG GCAGAGCATG AAACTCTTAT AGAGGAGGCA
    TCAATACAGG AGGTGGCCTT TGCAGAGGCA CCCATCGCTC AATCAGAGAT ACCAAAATCT
    CTTGCCTCCC AGCCTCTAGC AGAAATCTCT ACCCCTGAAC AGATTTCTAA ACTTGACGCA
    CAAGTGCCAT CTTTGCAACA AGAGGAGTTA CTTACAGAAA CTGTTGAACT TAATTTAAGC
    AATCAGGCTC TTCCTTCACA AATTTCTGAG CCTGAACCTT TTGAACCTGA GGCTTCAGCT
    TCAGATCCTC TACATGAGGA GCCCACAGAG CCCACAGTTT CAACAGAAGA ACCACAAGCT
    AATGGACTGT CTGAGGAGAC ACCAGTATTA CCTGTACAGC CACAACCAAC ATCTGTTTGC
    CAGACTGAGC CCTCCCTAGA ATCCCCCATC GCTCAGCCTG AGGAGCTGCT TCTAACAAAT
    GGAGGTGGAC CACCTGGCCT ACAAGAAAAT GATGCACCTG ATTCACAGGT TGAAGCTCAT
    GTTAGCCCAA TCACTGAGCC TGAGGACCAA CCTGTGACAG TCTCTGATAA CGTGCTTGTG
    GAAGAGGATG TAGAAAAAAC ATTGCCTGCG GATACGGAAG TCGTGGCTTC AACCCAAGTT
    GTAAAGCAGA GCTCCGAGAG TCAGTCTCAG GTTGCTGTTG CTGCACCCAA GAAAAAGCGA
    AAAATGAAGG AACTAAACAA GAAAGAGGCA GTGGGAGATC TCCTTGATGC GTTCAAAGAG
    GCTGGTGATG GCTCTTCTGA TGTGGATTCT TCCTCTAAGC CTACCAGTGC TGCACCTGAA
    CCAGATCAGA GCCCTTGTGC TCCTCCTGAG GAACAGGATG AAACTTGGGA AGAGAAGGAA
    GATAAACTGG ATGCAGAGAA TATTAAACCA GGAGAACATA AATATAAATA CAAAGAAGAG
    CAATGGAAAC CCCTAAACCC GGAGGAAAAG AAGAGTTATG ATCGCGAGTT TCTGCTAGGG
    TTCCAGTTTA TTGTGGCCAG TATGCAGAAA CCTGCGGGTC TTCCTCAAAT TACTGATGTG
    GTGCTGGATA AGGCAAACAA AACTCCATTG CGACCATTGG ATCCTACACG TCTGACGGGA
    ATGAACTGTG GGCCAGATTT TACACCTTCA TTTGCCAACC TTGGTCGGCC TGCTGTGGGT
    AACCGTGGAC CACCAGCTGG CATGGGTCCG AGACGGTCCC AGCAGGGTCC ACGAAAAGAG
    CCTCGTAAAA TTATTCAAAC CGTATCATTA AATGAGAATG TACAACTAAA CAAAGCTGAA
    AAGGCATGGA AACCGATAAA CAAGAGAGCT GGCGATGAGG AGGACCCAGA AAATATTAAG
    ACACAGGACC TATTCCGAAG GGTTCGCAGT ATCTTGAATA AGCTGACCCC TCAGATGTTC
    CAGCAGCTGA TGAAGCAGGT CACTGACCTT ACTATTGATA CCGAAGATCG TCTTAAGGGT
    GTCATTGACC TAATTTTTGA AAAAGCCATT TCAGAACCCA ATTTCTCTGT TGCATATGCC
    AACATGTGTC GTTGCCTTAT GGGGCTTAAA GTTCCAACCA CAGACAAGCC AGGGGTTACA
    GTCAACTTCC GCAAGTTGTT ACTGAATCGG TGCCAGAAGG AGTTTGAGAA GGATAAGGAT
    GATGATGAAG TGTTTGAGAA GAAACAAAAA GAAATGGATG CTGCTGCTTC GCCGGAGGAA
    AAAGCACGTT TGAATGATGA ACTGGTCGAG GCCCGTGACA GTGCACGACG GCGATCCTTG
    GGGAACATTA AGTTCATTGG AGAACTGTTT AAGTTAAAAA TGCTGACTGA AGCTATAATG
    CACGATTGTG TGGTGAAGCT ATTAAAAAAT CATGATGAGG AATCTCTGGA GTGCCTCTGC
    CGACTGCTGT CCACCATTGG CAAGGACCTG GATTTTGAGA GAGCTAAGCC ACGTATGGAC
    CAGTACTTCA ACCAGATGGA TAAAATCATT AAGGAGCGGA AGACATCTTC ACGAATTAGA
    TTCATGCTGC AGGATGTGAT TGATTTGCGT CTGTGTAATT GGGTGCCTCG TCGTGCAGAT
    CAGGGCCCTA AAACTATAGA TCAGATTCAT AAAGAAGCAG AAATGGAAAA GCAGAGGGAG
    CAGGTTAAGG TGCAACAACT GATGTCTAAA CAAGACAAGC GGCGAGAGCC CCCAGGACGA
    CCCACTGGAA GCCGGGGTAA CCAGCCTGCA GATGATGGCT GGAATACAGT GCCCATCAGT
    AAGGGCAGCC GTCCTATTGA CACCACTAGG ATCACAAAGA TAACAAAGCT TGGTGCTATT
    GATTCAAATC AACTGCTTGC CCCAGGAGGA CGTCTGAGTT GGGGTAAAGG AAGCAGCGGA
    GGAACTGGAG CCAAACCAGC AGAAACAGCT CCAGAATCTA GTCGGCCTGC GACCAGCACC
    TTAAACAGGT TTTCTGCCTT GCAGCAGTCT TCATCTGCTG AGAGTCAAGA TGCTAGACGG
    CCCTTGCATC GGGGAAGTTC TAGTCGGGAA CGTTCTGAAA AAGATCGTGG AGACAGATTT
    GATAGAGGTG AAAGGTTTGA TAAGGGAGAA CTGTTTGATA GATCTGACCG TTCAGATCGA
    GCTCGTCCTC TTCTTACAAA GAGAAGCTAT AGCAAGGAAA CTGAGGACCG TAGCCGAGAA
    AGAGAGGCCC ATATACCACA GGAGGCTGTG AGGAAGGTGG CAAGTATGAC TGAGCAGCGG
    GACCGCAGCA AAGAAAGTGT GAAAAAAGAA ATTCCAGCAC CGACAGCAGT GGTTGCCTCA
    TCAAAGCCCT CTATGTCTGA GGATGAGCTA GAGAAGAAAT CTAAAGCCAT CATAGAAGAA
    TATTTGCACA TTAATGATAT GAAGGAAGCT CTTCAGTGTG TAGTGGAGCT TAATAGTTCA
    ACGCTGCTTT TTATCTTTGT GCGGAATGGG ATTGAGTCAA CACTAGAGAG AAGCACTATT
    GCCAGAGAGC ATATGGGTCT CCTTTTGTAC CAGTTAGTAA AGGCAGGAAC TCTGTCCAAG
    GAGCAGTATT ACAAAGGGGT GCTTGAAGTT CTTGAAGTAG CAGAAGATAT GGAAATAGAT
    ATACCCCACA TCTGGCTGTA TTTAGCAGAG ATAATCAGCC CTGTGCTGCT AGAGGGGGGG
    ATTCCGATGG GGGAGCTGTT CAGAGAGCTG ACCAAACCTT TAGTGCTCAT TGGCAAAGCT
    GGAGTCCTTC TGGCAGAAAT CCTTGGATTA TTGTGCAAAG GCATGAGTCA TAAGAAGGCT
    GGAGCCCTGT GGAAAGAATC TGGGCTAAGC TGGAAGGAAT TCTTGTCCAA GGACCAGGAT
    ATTAACAAGT TTATAACTGA CAAGAAACTC GAATACACAA CAGGAGAGGA GACTGAAACA
    CCCAGTAAGA AAGACCTCAC CCCTGCTGAA CTTGATAAAA TTTTGACAAA ACTTTTGAAA
    GAGAACAGCA GTAACCAGAA GATATTTGAC TGGATTGAAG CCAACTTAAA TGACCAGCAA
    ACTTCATCCA ACACCTTTGT TCGAACTTTA ATGAGTACTG TGTGCCACTC TGCTATTATT
    TTTGAGAATC CTTGTAGGGT TGATGTTAAT ATTATCCATA CTAGAGCCAA ATTTCTTCAG
    AAATATTTAA GTGACGAGGC CAAGGAACTA CAGGCTCTCT ATGCTCTACA GGCACTTGTT
    GTAAAGCTTG ACCAGCCCCC AAATCTCCTA CGGATGTTTT TTGATGCTCT CTATGATGAG
    GATGTGATAA AAGAGGATCC ATTCTACAAG TGGGAGTCCA GCAAAGACCC AGCTGAACAG
    CAAGGAAAAG GTGTTGCTCT TAAATCTGTC ACTGCCTTCT TCACATGGCT ACGGGAAGCA
    GAGGATGAGT CTGATAATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa10204
    Clone ID Related Accession (Same CDS sequence) XM_018094420.1 , XM_004914398.4 , XM_004914398.3 , XM_002935144.5 , XM_018094419.1 , XM_012963377.3 , XM_002935144.4 , XM_012963377.2
    Accession Version XM_018094420.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4830bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
    CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA
    CAAATGAATA CACAATCTCA GCCTCGACAG GCAGGATTCA GATCAATCCA GCATTATTAT
    CAAAATAGGA CACAGCCTCC CACTAGTGCC ACCAGAGTGC AGAGTGGTTC TTCTGCTCGA
    GCAGTTACTC CAGCCCATGT GTATCAACCA GGCTCCCAAG TTATGATGAT TCCTTCACAG
    ATTTCATACC CTTCCCAGGG ATACTACATT CAGGGACAGG GACGCTCATA TGTGGTGCCC
    ACAAACCAGT ACCCTGTGCA GACTGGGGCT CCAGGTTTCT TCCCCAGTGC AAGTCCCTCA
    GAATATGGCA CATATGCTGG AGCATATTAC CCTGCACAAC AGACGTTCCC TCCTGGAGTG
    CCTGGAGCAC AAGTTATGAT GAATCAGCCA CAGGTGATCC CTCCTAAACG GGAGCGCAAA
    ACGATAAGAA TTAGAGACCC CAACCAAGGA GGCAAAGATA TCACAGAAGA AATTCTCTCA
    GGAGGAAGAA CATCCTCTAC GCCAACGCCT CCACAGTCAA CTGTGGGAAG TTCTGAAATT
    CAAGCTAATG GAGAAAGCGT TCATCATGTT ACCGTTGTTG TGCGCCCAGA TGATGGACCC
    AAGGCCACCC AAGTGCTGAT CAAAGCCTCA GAGAGCCCTT CCCCTCCTCT GCCTCTCTCT
    GAAGCTGAGC CAAGCATAGC AAATGTGTGT GATATTTCAA AAGATAACAC TATCTCTGAG
    TTGACTTCTC ATATAGTAGA GCCCCTAAGT TCAGAAGAGA CAACCATTGA TACTTCTACC
    TCTCAGGTGG ATCTCAAACA CCAGCCAGTG GCTGCTATGG CAGAGCATGA AACTCTTATA
    GAGGAGGCAT CAATACAGGA GGTGGCCTTT GCAGAGGCAC CCATCGCTCA ATCAGAGATA
    CCAAAATCTC TTGCCTCCCA GCCTCTAGCA GAAATCTCTA CCCCTGAACA GATTTCTAAA
    CTTGACGCAC AAGTGCCATC TTTGCAACAA GAGGAGTTAC TTACAGAAAC TGTTGAACTT
    AATTTAAGCA ATCAGGCTCT TCCTTCACAA ATTTCTGAGC CTGAACCTTT TGAACCTGAG
    GCTTCAGCTT CAGATCCTCT ACATGAGGAG CCCACAGAGC CCACAGTTTC AACAGAAGAA
    CCACAAGCTA ATGGACTGTC TGAGGAGACA CCAGTATTAC CTGTACAGCC ACAACCAACA
    TCTGTTTGCC AGACTGAGCC CTCCCTAGAA TCCCCCATCG CTCAGCCTGA GGAGCTGCTT
    CTAACAAATG GAGGTGGACC ACCTGGCCTA CAAGAAAATG ATGCACCTGA TTCACAGGTT
    GAAGCTCATG TTAGCCCAAT CACTGAGCCT GAGGACCAAC CTGTGACAGT CTCTGATAAC
    GTGCTTGTGG AAGAGGATGT AGAAAAAACA TTGCCTGCGG ATACGGAAGT CGTGGCTTCA
    ACCCAAGTTG TAAAGCAGAG CTCCGAGAGT CAGTCTCAGG TTGCTGTTGC TGCACCCAAG
    AAAAAGCGAA AAATGAAGGA ACTAAACAAG AAAGAGGCAG TGGGAGATCT CCTTGATGCG
    TTCAAAGAGG CTGGTGATGG CTCTTCTGAT GTGGATTCTT CCTCTAAGCC TACCAGTGCT
    GCACCTGAAC CAGATCAGAG CCCTTGTGCT CCTCCTGAGG AACAGGATGA AACTTGGGAA
    GAGAAGGAAG ATAAACTGGA TGCAGAGAAT ATTAAACCAG GAGAACATAA ATATAAATAC
    AAAGAAGAGC AATGGAAACC CCTAAACCCG GAGGAAAAGA AGAGTTATGA TCGCGAGTTT
    CTGCTAGGGT TCCAGTTTAT TGTGGCCAGT ATGCAGAAAC CTGCGGGTCT TCCTCAAATT
    ACTGATGTGG TGCTGGATAA GGCAAACAAA ACTCCATTGC GACCATTGGA TCCTACACGT
    CTGACGGGAA TGAACTGTGG GCCAGATTTT ACACCTTCAT TTGCCAACCT TGGTCGGCCT
    GCTGTGGGTA ACCGTGGACC ACCAGCTGGC ATGGGTCCGA GACGGTCCCA GCAGGGTCCA
    CGAAAAGAGC CTCGTAAAAT TATTCAAACC GTATCATTAA ATGAGAATGT ACAACTAAAC
    AAAGCTGAAA AGGCATGGAA ACCGATAAAC AAGAGAGCTG GCGATGAGGA GGACCCAGAA
    AATATTAAGA CACAGGACCT ATTCCGAAGG GTTCGCAGTA TCTTGAATAA GCTGACCCCT
    CAGATGTTCC AGCAGCTGAT GAAGCAGGTC ACTGACCTTA CTATTGATAC CGAAGATCGT
    CTTAAGGGTG TCATTGACCT AATTTTTGAA AAAGCCATTT CAGAACCCAA TTTCTCTGTT
    GCATATGCCA ACATGTGTCG TTGCCTTATG GGGCTTAAAG TTCCAACCAC AGACAAGCCA
    GGGGTTACAG TCAACTTCCG CAAGTTGTTA CTGAATCGGT GCCAGAAGGA GTTTGAGAAG
    GATAAGGATG ATGATGAAGT GTTTGAGAAG AAACAAAAAG AAATGGATGC TGCTGCTTCG
    CCGGAGGAAA AAGCACGTTT GAATGATGAA CTGGTCGAGG CCCGTGACAG TGCACGACGG
    CGATCCTTGG GGAACATTAA GTTCATTGGA GAACTGTTTA AGTTAAAAAT GCTGACTGAA
    GCTATAATGC ACGATTGTGT GGTGAAGCTA TTAAAAAATC ATGATGAGGA ATCTCTGGAG
    TGCCTCTGCC GACTGCTGTC CACCATTGGC AAGGACCTGG ATTTTGAGAG AGCTAAGCCA
    CGTATGGACC AGTACTTCAA CCAGATGGAT AAAATCATTA AGGAGCGGAA GACATCTTCA
    CGAATTAGAT TCATGCTGCA GGATGTGATT GATTTGCGTC TGTGTAATTG GGTGCCTCGT
    CGTGCAGATC AGGGCCCTAA AACTATAGAT CAGATTCATA AAGAAGCAGA AATGGAAAAG
    CAGAGGGAGC AGGTTAAGGT GCAACAACTG ATGTCTAAAC AAGACAAGCG GCGAGAGCCC
    CCAGGACGAC CCACTGGAAG CCGGGGTAAC CAGCCTGCAG ATGATGGCTG GAATACAGTG
    CCCATCAGTA AGGGCAGCCG TCCTATTGAC ACCACTAGGA TCACAAAGAT AACAAAGCTT
    GGTGCTATTG ATTCAAATCA ACTGCTTGCC CCAGGAGGAC GTCTGAGTTG GGGTAAAGGA
    AGCAGCGGAG GAACTGGAGC CAAACCAGCA GAAACAGCTC CAGAATCTAG TCGGCCTGCG
    ACCAGCACCT TAAACAGGTT TTCTGCCTTG CAGCAGTCTT CATCTGCTGA GAGTCAAGAT
    GCTAGACGGC CCTTGCATCG GGGAAGTTCT AGTCGGGAAC GTTCTGAAAA AGATCGTGGA
    GACAGATTTG ATAGAGGTGA AAGGTTTGAT AAGGGAGAAC TGTTTGATAG ATCTGACCGT
    TCAGATCGAG CTCGTCCTCT TCTTACAAAG AGAAGCTATA GCAAGGAAAC TGAGGACCGT
    AGCCGAGAAA GAGAGGCCCA TATACCACAG GAGGCTGTGA GGAAGGTGGC AAGTATGACT
    GAGCAGCGGG ACCGCAGCAA AGAAAGTGTG AAAAAAGAAA TTCCAGCACC GACAGCAGTG
    GTTGCCTCAT CAAAGCCCTC TATGTCTGAG GATGAGCTAG AGAAGAAATC TAAAGCCATC
    ATAGAAGAAT ATTTGCACAT TAATGATATG AAGGAAGCTC TTCAGTGTGT AGTGGAGCTT
    AATAGTTCAA CGCTGCTTTT TATCTTTGTG CGGAATGGGA TTGAGTCAAC ACTAGAGAGA
    AGCACTATTG CCAGAGAGCA TATGGGTCTC CTTTTGTACC AGTTAGTAAA GGCAGGAACT
    CTGTCCAAGG AGCAGTATTA CAAAGGGGTG CTTGAAGTTC TTGAAGTAGC AGAAGATATG
    GAAATAGATA TACCCCACAT CTGGCTGTAT TTAGCAGAGA TAATCAGCCC TGTGCTGCTA
    GAGGGGGGGA TTCCGATGGG GGAGCTGTTC AGAGAGCTGA CCAAACCTTT AGTGCTCATT
    GGCAAAGCTG GAGTCCTTCT GGCAGAAATC CTTGGATTAT TGTGCAAAGG CATGAGTCAT
    AAGAAGGCTG GAGCCCTGTG GAAAGAATCT GGGCTAAGCT GGAAGGAATT CTTGTCCAAG
    GACCAGGATA TTAACAAGTT TATAACTGAC AAGAAACTCG AATACACAAC AGGAGAGGAG
    ACTGAAACAC CCAGTAAGAA AGACCTCACC CCTGCTGAAC TTGATAAAAT TTTGACAAAA
    CTTTTGAAAG AGAACAGCAG TAACCAGAAG ATATTTGACT GGATTGAAGC CAACTTAAAT
    GACCAGCAAA CTTCATCCAA CACCTTTGTT CGAACTTTAA TGAGTACTGT GTGCCACTCT
    GCTATTATTT TTGAGAATCC TTGTAGGGTT GATGTTAATA TTATCCATAC TAGAGCCAAA
    TTTCTTCAGA AATATTTAAG TGACGAGGCC AAGGAACTAC AGGCTCTCTA TGCTCTACAG
    GCACTTGTTG TAAAGCTTGA CCAGCCCCCA AATCTCCTAC GGATGTTTTT TGATGCTCTC
    TATGATGAGG ATGTGATAAA AGAGGATCCA TTCTACAAGT GGGAGTCCAG CAAAGACCCA
    GCTGAACAGC AAGGAAAAGG TGTTGCTCTT AAATCTGTCA CTGCCTTCTT CACATGGCTA
    CGGGAAGCAG AGGATGAGTC TGATAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017949909.1
    CDS491..5320
    Translation

    Target ORF information:

    RefSeq Version XM_018094420.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018094420.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
    CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA
    CAAATGAATA CACAATCTCA GCCTCGACAG GCAGGATTCA GATCAATCCA GCATTATTAT
    CAAAATAGGA CACAGCCTCC CACTAGTGCC ACCAGAGTGC AGAGTGGTTC TTCTGCTCGA
    GCAGTTACTC CAGCCCATGT GTATCAACCA GGCTCCCAAG TTATGATGAT TCCTTCACAG
    ATTTCATACC CTTCCCAGGG ATACTACATT CAGGGACAGG GACGCTCATA TGTGGTGCCC
    ACAAACCAGT ACCCTGTGCA GACTGGGGCT CCAGGTTTCT TCCCCAGTGC AAGTCCCTCA
    GAATATGGCA CATATGCTGG AGCATATTAC CCTGCACAAC AGACGTTCCC TCCTGGAGTG
    CCTGGAGCAC AAGTTATGAT GAATCAGCCA CAGGTGATCC CTCCTAAACG GGAGCGCAAA
    ACGATAAGAA TTAGAGACCC CAACCAAGGA GGCAAAGATA TCACAGAAGA AATTCTCTCA
    GGAGGAAGAA CATCCTCTAC GCCAACGCCT CCACAGTCAA CTGTGGGAAG TTCTGAAATT
    CAAGCTAATG GAGAAAGCGT TCATCATGTT ACCGTTGTTG TGCGCCCAGA TGATGGACCC
    AAGGCCACCC AAGTGCTGAT CAAAGCCTCA GAGAGCCCTT CCCCTCCTCT GCCTCTCTCT
    GAAGCTGAGC CAAGCATAGC AAATGTGTGT GATATTTCAA AAGATAACAC TATCTCTGAG
    TTGACTTCTC ATATAGTAGA GCCCCTAAGT TCAGAAGAGA CAACCATTGA TACTTCTACC
    TCTCAGGTGG ATCTCAAACA CCAGCCAGTG GCTGCTATGG CAGAGCATGA AACTCTTATA
    GAGGAGGCAT CAATACAGGA GGTGGCCTTT GCAGAGGCAC CCATCGCTCA ATCAGAGATA
    CCAAAATCTC TTGCCTCCCA GCCTCTAGCA GAAATCTCTA CCCCTGAACA GATTTCTAAA
    CTTGACGCAC AAGTGCCATC TTTGCAACAA GAGGAGTTAC TTACAGAAAC TGTTGAACTT
    AATTTAAGCA ATCAGGCTCT TCCTTCACAA ATTTCTGAGC CTGAACCTTT TGAACCTGAG
    GCTTCAGCTT CAGATCCTCT ACATGAGGAG CCCACAGAGC CCACAGTTTC AACAGAAGAA
    CCACAAGCTA ATGGACTGTC TGAGGAGACA CCAGTATTAC CTGTACAGCC ACAACCAACA
    TCTGTTTGCC AGACTGAGCC CTCCCTAGAA TCCCCCATCG CTCAGCCTGA GGAGCTGCTT
    CTAACAAATG GAGGTGGACC ACCTGGCCTA CAAGAAAATG ATGCACCTGA TTCACAGGTT
    GAAGCTCATG TTAGCCCAAT CACTGAGCCT GAGGACCAAC CTGTGACAGT CTCTGATAAC
    GTGCTTGTGG AAGAGGATGT AGAAAAAACA TTGCCTGCGG ATACGGAAGT CGTGGCTTCA
    ACCCAAGTTG TAAAGCAGAG CTCCGAGAGT CAGTCTCAGG TTGCTGTTGC TGCACCCAAG
    AAAAAGCGAA AAATGAAGGA ACTAAACAAG AAAGAGGCAG TGGGAGATCT CCTTGATGCG
    TTCAAAGAGG CTGGTGATGG CTCTTCTGAT GTGGATTCTT CCTCTAAGCC TACCAGTGCT
    GCACCTGAAC CAGATCAGAG CCCTTGTGCT CCTCCTGAGG AACAGGATGA AACTTGGGAA
    GAGAAGGAAG ATAAACTGGA TGCAGAGAAT ATTAAACCAG GAGAACATAA ATATAAATAC
    AAAGAAGAGC AATGGAAACC CCTAAACCCG GAGGAAAAGA AGAGTTATGA TCGCGAGTTT
    CTGCTAGGGT TCCAGTTTAT TGTGGCCAGT ATGCAGAAAC CTGCGGGTCT TCCTCAAATT
    ACTGATGTGG TGCTGGATAA GGCAAACAAA ACTCCATTGC GACCATTGGA TCCTACACGT
    CTGACGGGAA TGAACTGTGG GCCAGATTTT ACACCTTCAT TTGCCAACCT TGGTCGGCCT
    GCTGTGGGTA ACCGTGGACC ACCAGCTGGC ATGGGTCCGA GACGGTCCCA GCAGGGTCCA
    CGAAAAGAGC CTCGTAAAAT TATTCAAACC GTATCATTAA ATGAGAATGT ACAACTAAAC
    AAAGCTGAAA AGGCATGGAA ACCGATAAAC AAGAGAGCTG GCGATGAGGA GGACCCAGAA
    AATATTAAGA CACAGGACCT ATTCCGAAGG GTTCGCAGTA TCTTGAATAA GCTGACCCCT
    CAGATGTTCC AGCAGCTGAT GAAGCAGGTC ACTGACCTTA CTATTGATAC CGAAGATCGT
    CTTAAGGGTG TCATTGACCT AATTTTTGAA AAAGCCATTT CAGAACCCAA TTTCTCTGTT
    GCATATGCCA ACATGTGTCG TTGCCTTATG GGGCTTAAAG TTCCAACCAC AGACAAGCCA
    GGGGTTACAG TCAACTTCCG CAAGTTGTTA CTGAATCGGT GCCAGAAGGA GTTTGAGAAG
    GATAAGGATG ATGATGAAGT GTTTGAGAAG AAACAAAAAG AAATGGATGC TGCTGCTTCG
    CCGGAGGAAA AAGCACGTTT GAATGATGAA CTGGTCGAGG CCCGTGACAG TGCACGACGG
    CGATCCTTGG GGAACATTAA GTTCATTGGA GAACTGTTTA AGTTAAAAAT GCTGACTGAA
    GCTATAATGC ACGATTGTGT GGTGAAGCTA TTAAAAAATC ATGATGAGGA ATCTCTGGAG
    TGCCTCTGCC GACTGCTGTC CACCATTGGC AAGGACCTGG ATTTTGAGAG AGCTAAGCCA
    CGTATGGACC AGTACTTCAA CCAGATGGAT AAAATCATTA AGGAGCGGAA GACATCTTCA
    CGAATTAGAT TCATGCTGCA GGATGTGATT GATTTGCGTC TGTGTAATTG GGTGCCTCGT
    CGTGCAGATC AGGGCCCTAA AACTATAGAT CAGATTCATA AAGAAGCAGA AATGGAAAAG
    CAGAGGGAGC AGGTTAAGGT GCAACAACTG ATGTCTAAAC AAGACAAGCG GCGAGAGCCC
    CCAGGACGAC CCACTGGAAG CCGGGGTAAC CAGCCTGCAG ATGATGGCTG GAATACAGTG
    CCCATCAGTA AGGGCAGCCG TCCTATTGAC ACCACTAGGA TCACAAAGAT AACAAAGCTT
    GGTGCTATTG ATTCAAATCA ACTGCTTGCC CCAGGAGGAC GTCTGAGTTG GGGTAAAGGA
    AGCAGCGGAG GAACTGGAGC CAAACCAGCA GAAACAGCTC CAGAATCTAG TCGGCCTGCG
    ACCAGCACCT TAAACAGGTT TTCTGCCTTG CAGCAGTCTT CATCTGCTGA GAGTCAAGAT
    GCTAGACGGC CCTTGCATCG GGGAAGTTCT AGTCGGGAAC GTTCTGAAAA AGATCGTGGA
    GACAGATTTG ATAGAGGTGA AAGGTTTGAT AAGGGAGAAC TGTTTGATAG ATCTGACCGT
    TCAGATCGAG CTCGTCCTCT TCTTACAAAG AGAAGCTATA GCAAGGAAAC TGAGGACCGT
    AGCCGAGAAA GAGAGGCCCA TATACCACAG GAGGCTGTGA GGAAGGTGGC AAGTATGACT
    GAGCAGCGGG ACCGCAGCAA AGAAAGTGTG AAAAAAGAAA TTCCAGCACC GACAGCAGTG
    GTTGCCTCAT CAAAGCCCTC TATGTCTGAG GATGAGCTAG AGAAGAAATC TAAAGCCATC
    ATAGAAGAAT ATTTGCACAT TAATGATATG AAGGAAGCTC TTCAGTGTGT AGTGGAGCTT
    AATAGTTCAA CGCTGCTTTT TATCTTTGTG CGGAATGGGA TTGAGTCAAC ACTAGAGAGA
    AGCACTATTG CCAGAGAGCA TATGGGTCTC CTTTTGTACC AGTTAGTAAA GGCAGGAACT
    CTGTCCAAGG AGCAGTATTA CAAAGGGGTG CTTGAAGTTC TTGAAGTAGC AGAAGATATG
    GAAATAGATA TACCCCACAT CTGGCTGTAT TTAGCAGAGA TAATCAGCCC TGTGCTGCTA
    GAGGGGGGGA TTCCGATGGG GGAGCTGTTC AGAGAGCTGA CCAAACCTTT AGTGCTCATT
    GGCAAAGCTG GAGTCCTTCT GGCAGAAATC CTTGGATTAT TGTGCAAAGG CATGAGTCAT
    AAGAAGGCTG GAGCCCTGTG GAAAGAATCT GGGCTAAGCT GGAAGGAATT CTTGTCCAAG
    GACCAGGATA TTAACAAGTT TATAACTGAC AAGAAACTCG AATACACAAC AGGAGAGGAG
    ACTGAAACAC CCAGTAAGAA AGACCTCACC CCTGCTGAAC TTGATAAAAT TTTGACAAAA
    CTTTTGAAAG AGAACAGCAG TAACCAGAAG ATATTTGACT GGATTGAAGC CAACTTAAAT
    GACCAGCAAA CTTCATCCAA CACCTTTGTT CGAACTTTAA TGAGTACTGT GTGCCACTCT
    GCTATTATTT TTGAGAATCC TTGTAGGGTT GATGTTAATA TTATCCATAC TAGAGCCAAA
    TTTCTTCAGA AATATTTAAG TGACGAGGCC AAGGAACTAC AGGCTCTCTA TGCTCTACAG
    GCACTTGTTG TAAAGCTTGA CCAGCCCCCA AATCTCCTAC GGATGTTTTT TGATGCTCTC
    TATGATGAGG ATGTGATAAA AGAGGATCCA TTCTACAAGT GGGAGTCCAG CAAAGACCCA
    GCTGAACAGC AAGGAAAAGG TGTTGCTCTT AAATCTGTCA CTGCCTTCTT CACATGGCTA
    CGGGAAGCAG AGGATGAGTC TGATAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa10204
    Clone ID Related Accession (Same CDS sequence) XM_018094420.1 , XM_004914398.4 , XM_004914398.3 , XM_002935144.5 , XM_018094419.1 , XM_012963377.3 , XM_002935144.4 , XM_012963377.2
    Accession Version XM_004914398.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4830bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004914398.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
    CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA
    CAAATGAATA CACAATCTCA GCCTCGACAG GCAGGATTCA GATCAATCCA GCATTATTAT
    CAAAATAGGA CACAGCCTCC CACTAGTGCC ACCAGAGTGC AGAGTGGTTC TTCTGCTCGA
    GCAGTTACTC CAGCCCATGT GTATCAACCA GGCTCCCAAG TTATGATGAT TCCTTCACAG
    ATTTCATACC CTTCCCAGGG ATACTACATT CAGGGACAGG GACGCTCATA TGTGGTGCCC
    ACAAACCAGT ACCCTGTGCA GACTGGGGCT CCAGGTTTCT TCCCCAGTGC AAGTCCCTCA
    GAATATGGCA CATATGCTGG AGCATATTAC CCTGCACAAC AGACGTTCCC TCCTGGAGTG
    CCTGGAGCAC AAGTTATGAT GAATCAGCCA CAGGTGATCC CTCCTAAACG GGAGCGCAAA
    ACGATAAGAA TTAGAGACCC CAACCAAGGA GGCAAAGATA TCACAGAAGA AATTCTCTCA
    GGAGGAAGAA CATCCTCTAC GCCAACGCCT CCACAGTCAA CTGTGGGAAG TTCTGAAATT
    CAAGCTAATG GAGAAAGCGT TCATCATGTT ACCGTTGTTG TGCGCCCAGA TGATGGACCC
    AAGGCCACCC AAGTGCTGAT CAAAGCCTCA GAGAGCCCTT CCCCTCCTCT GCCTCTCTCT
    GAAGCTGAGC CAAGCATAGC AAATGTGTGT GATATTTCAA AAGATAACAC TATCTCTGAG
    TTGACTTCTC ATATAGTAGA GCCCCTAAGT TCAGAAGAGA CAACCATTGA TACTTCTACC
    TCTCAGGTGG ATCTCAAACA CCAGCCAGTG GCTGCTATGG CAGAGCATGA AACTCTTATA
    GAGGAGGCAT CAATACAGGA GGTGGCCTTT GCAGAGGCAC CCATCGCTCA ATCAGAGATA
    CCAAAATCTC TTGCCTCCCA GCCTCTAGCA GAAATCTCTA CCCCTGAACA GATTTCTAAA
    CTTGACGCAC AAGTGCCATC TTTGCAACAA GAGGAGTTAC TTACAGAAAC TGTTGAACTT
    AATTTAAGCA ATCAGGCTCT TCCTTCACAA ATTTCTGAGC CTGAACCTTT TGAACCTGAG
    GCTTCAGCTT CAGATCCTCT ACATGAGGAG CCCACAGAGC CCACAGTTTC AACAGAAGAA
    CCACAAGCTA ATGGACTGTC TGAGGAGACA CCAGTATTAC CTGTACAGCC ACAACCAACA
    TCTGTTTGCC AGACTGAGCC CTCCCTAGAA TCCCCCATCG CTCAGCCTGA GGAGCTGCTT
    CTAACAAATG GAGGTGGACC ACCTGGCCTA CAAGAAAATG ATGCACCTGA TTCACAGGTT
    GAAGCTCATG TTAGCCCAAT CACTGAGCCT GAGGACCAAC CTGTGACAGT CTCTGATAAC
    GTGCTTGTGG AAGAGGATGT AGAAAAAACA TTGCCTGCGG ATACGGAAGT CGTGGCTTCA
    ACCCAAGTTG TAAAGCAGAG CTCCGAGAGT CAGTCTCAGG TTGCTGTTGC TGCACCCAAG
    AAAAAGCGAA AAATGAAGGA ACTAAACAAG AAAGAGGCAG TGGGAGATCT CCTTGATGCG
    TTCAAAGAGG CTGGTGATGG CTCTTCTGAT GTGGATTCTT CCTCTAAGCC TACCAGTGCT
    GCACCTGAAC CAGATCAGAG CCCTTGTGCT CCTCCTGAGG AACAGGATGA AACTTGGGAA
    GAGAAGGAAG ATAAACTGGA TGCAGAGAAT ATTAAACCAG GAGAACATAA ATATAAATAC
    AAAGAAGAGC AATGGAAACC CCTAAACCCG GAGGAAAAGA AGAGTTATGA TCGCGAGTTT
    CTGCTAGGGT TCCAGTTTAT TGTGGCCAGT ATGCAGAAAC CTGCGGGTCT TCCTCAAATT
    ACTGATGTGG TGCTGGATAA GGCAAACAAA ACTCCATTGC GACCATTGGA TCCTACACGT
    CTGACGGGAA TGAACTGTGG GCCAGATTTT ACACCTTCAT TTGCCAACCT TGGTCGGCCT
    GCTGTGGGTA ACCGTGGACC ACCAGCTGGC ATGGGTCCGA GACGGTCCCA GCAGGGTCCA
    CGAAAAGAGC CTCGTAAAAT TATTCAAACC GTATCATTAA ATGAGAATGT ACAACTAAAC
    AAAGCTGAAA AGGCATGGAA ACCGATAAAC AAGAGAGCTG GCGATGAGGA GGACCCAGAA
    AATATTAAGA CACAGGACCT ATTCCGAAGG GTTCGCAGTA TCTTGAATAA GCTGACCCCT
    CAGATGTTCC AGCAGCTGAT GAAGCAGGTC ACTGACCTTA CTATTGATAC CGAAGATCGT
    CTTAAGGGTG TCATTGACCT AATTTTTGAA AAAGCCATTT CAGAACCCAA TTTCTCTGTT
    GCATATGCCA ACATGTGTCG TTGCCTTATG GGGCTTAAAG TTCCAACCAC AGACAAGCCA
    GGGGTTACAG TCAACTTCCG CAAGTTGTTA CTGAATCGGT GCCAGAAGGA GTTTGAGAAG
    GATAAGGATG ATGATGAAGT GTTTGAGAAG AAACAAAAAG AAATGGATGC TGCTGCTTCG
    CCGGAGGAAA AAGCACGTTT GAATGATGAA CTGGTCGAGG CCCGTGACAG TGCACGACGG
    CGATCCTTGG GGAACATTAA GTTCATTGGA GAACTGTTTA AGTTAAAAAT GCTGACTGAA
    GCTATAATGC ACGATTGTGT GGTGAAGCTA TTAAAAAATC ATGATGAGGA ATCTCTGGAG
    TGCCTCTGCC GACTGCTGTC CACCATTGGC AAGGACCTGG ATTTTGAGAG AGCTAAGCCA
    CGTATGGACC AGTACTTCAA CCAGATGGAT AAAATCATTA AGGAGCGGAA GACATCTTCA
    CGAATTAGAT TCATGCTGCA GGATGTGATT GATTTGCGTC TGTGTAATTG GGTGCCTCGT
    CGTGCAGATC AGGGCCCTAA AACTATAGAT CAGATTCATA AAGAAGCAGA AATGGAAAAG
    CAGAGGGAGC AGGTTAAGGT GCAACAACTG ATGTCTAAAC AAGACAAGCG GCGAGAGCCC
    CCAGGACGAC CCACTGGAAG CCGGGGTAAC CAGCCTGCAG ATGATGGCTG GAATACAGTG
    CCCATCAGTA AGGGCAGCCG TCCTATTGAC ACCACTAGGA TCACAAAGAT AACAAAGCTT
    GGTGCTATTG ATTCAAATCA ACTGCTTGCC CCAGGAGGAC GTCTGAGTTG GGGTAAAGGA
    AGCAGCGGAG GAACTGGAGC CAAACCAGCA GAAACAGCTC CAGAATCTAG TCGGCCTGCG
    ACCAGCACCT TAAACAGGTT TTCTGCCTTG CAGCAGTCTT CATCTGCTGA GAGTCAAGAT
    GCTAGACGGC CCTTGCATCG GGGAAGTTCT AGTCGGGAAC GTTCTGAAAA AGATCGTGGA
    GACAGATTTG ATAGAGGTGA AAGGTTTGAT AAGGGAGAAC TGTTTGATAG ATCTGACCGT
    TCAGATCGAG CTCGTCCTCT TCTTACAAAG AGAAGCTATA GCAAGGAAAC TGAGGACCGT
    AGCCGAGAAA GAGAGGCCCA TATACCACAG GAGGCTGTGA GGAAGGTGGC AAGTATGACT
    GAGCAGCGGG ACCGCAGCAA AGAAAGTGTG AAAAAAGAAA TTCCAGCACC GACAGCAGTG
    GTTGCCTCAT CAAAGCCCTC TATGTCTGAG GATGAGCTAG AGAAGAAATC TAAAGCCATC
    ATAGAAGAAT ATTTGCACAT TAATGATATG AAGGAAGCTC TTCAGTGTGT AGTGGAGCTT
    AATAGTTCAA CGCTGCTTTT TATCTTTGTG CGGAATGGGA TTGAGTCAAC ACTAGAGAGA
    AGCACTATTG CCAGAGAGCA TATGGGTCTC CTTTTGTACC AGTTAGTAAA GGCAGGAACT
    CTGTCCAAGG AGCAGTATTA CAAAGGGGTG CTTGAAGTTC TTGAAGTAGC AGAAGATATG
    GAAATAGATA TACCCCACAT CTGGCTGTAT TTAGCAGAGA TAATCAGCCC TGTGCTGCTA
    GAGGGGGGGA TTCCGATGGG GGAGCTGTTC AGAGAGCTGA CCAAACCTTT AGTGCTCATT
    GGCAAAGCTG GAGTCCTTCT GGCAGAAATC CTTGGATTAT TGTGCAAAGG CATGAGTCAT
    AAGAAGGCTG GAGCCCTGTG GAAAGAATCT GGGCTAAGCT GGAAGGAATT CTTGTCCAAG
    GACCAGGATA TTAACAAGTT TATAACTGAC AAGAAACTCG AATACACAAC AGGAGAGGAG
    ACTGAAACAC CCAGTAAGAA AGACCTCACC CCTGCTGAAC TTGATAAAAT TTTGACAAAA
    CTTTTGAAAG AGAACAGCAG TAACCAGAAG ATATTTGACT GGATTGAAGC CAACTTAAAT
    GACCAGCAAA CTTCATCCAA CACCTTTGTT CGAACTTTAA TGAGTACTGT GTGCCACTCT
    GCTATTATTT TTGAGAATCC TTGTAGGGTT GATGTTAATA TTATCCATAC TAGAGCCAAA
    TTTCTTCAGA AATATTTAAG TGACGAGGCC AAGGAACTAC AGGCTCTCTA TGCTCTACAG
    GCACTTGTTG TAAAGCTTGA CCAGCCCCCA AATCTCCTAC GGATGTTTTT TGATGCTCTC
    TATGATGAGG ATGTGATAAA AGAGGATCCA TTCTACAAGT GGGAGTCCAG CAAAGACCCA
    GCTGAACAGC AAGGAAAAGG TGTTGCTCTT AAATCTGTCA CTGCCTTCTT CACATGGCTA
    CGGGAAGCAG AGGATGAGTC TGATAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004914455.1
    CDS195..5024
    Translation

    Target ORF information:

    RefSeq Version XM_004914398.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004914398.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
    CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA
    CAAATGAATA CACAATCTCA GCCTCGACAG GCAGGATTCA GATCAATCCA GCATTATTAT
    CAAAATAGGA CACAGCCTCC CACTAGTGCC ACCAGAGTGC AGAGTGGTTC TTCTGCTCGA
    GCAGTTACTC CAGCCCATGT GTATCAACCA GGCTCCCAAG TTATGATGAT TCCTTCACAG
    ATTTCATACC CTTCCCAGGG ATACTACATT CAGGGACAGG GACGCTCATA TGTGGTGCCC
    ACAAACCAGT ACCCTGTGCA GACTGGGGCT CCAGGTTTCT TCCCCAGTGC AAGTCCCTCA
    GAATATGGCA CATATGCTGG AGCATATTAC CCTGCACAAC AGACGTTCCC TCCTGGAGTG
    CCTGGAGCAC AAGTTATGAT GAATCAGCCA CAGGTGATCC CTCCTAAACG GGAGCGCAAA
    ACGATAAGAA TTAGAGACCC CAACCAAGGA GGCAAAGATA TCACAGAAGA AATTCTCTCA
    GGAGGAAGAA CATCCTCTAC GCCAACGCCT CCACAGTCAA CTGTGGGAAG TTCTGAAATT
    CAAGCTAATG GAGAAAGCGT TCATCATGTT ACCGTTGTTG TGCGCCCAGA TGATGGACCC
    AAGGCCACCC AAGTGCTGAT CAAAGCCTCA GAGAGCCCTT CCCCTCCTCT GCCTCTCTCT
    GAAGCTGAGC CAAGCATAGC AAATGTGTGT GATATTTCAA AAGATAACAC TATCTCTGAG
    TTGACTTCTC ATATAGTAGA GCCCCTAAGT TCAGAAGAGA CAACCATTGA TACTTCTACC
    TCTCAGGTGG ATCTCAAACA CCAGCCAGTG GCTGCTATGG CAGAGCATGA AACTCTTATA
    GAGGAGGCAT CAATACAGGA GGTGGCCTTT GCAGAGGCAC CCATCGCTCA ATCAGAGATA
    CCAAAATCTC TTGCCTCCCA GCCTCTAGCA GAAATCTCTA CCCCTGAACA GATTTCTAAA
    CTTGACGCAC AAGTGCCATC TTTGCAACAA GAGGAGTTAC TTACAGAAAC TGTTGAACTT
    AATTTAAGCA ATCAGGCTCT TCCTTCACAA ATTTCTGAGC CTGAACCTTT TGAACCTGAG
    GCTTCAGCTT CAGATCCTCT ACATGAGGAG CCCACAGAGC CCACAGTTTC AACAGAAGAA
    CCACAAGCTA ATGGACTGTC TGAGGAGACA CCAGTATTAC CTGTACAGCC ACAACCAACA
    TCTGTTTGCC AGACTGAGCC CTCCCTAGAA TCCCCCATCG CTCAGCCTGA GGAGCTGCTT
    CTAACAAATG GAGGTGGACC ACCTGGCCTA CAAGAAAATG ATGCACCTGA TTCACAGGTT
    GAAGCTCATG TTAGCCCAAT CACTGAGCCT GAGGACCAAC CTGTGACAGT CTCTGATAAC
    GTGCTTGTGG AAGAGGATGT AGAAAAAACA TTGCCTGCGG ATACGGAAGT CGTGGCTTCA
    ACCCAAGTTG TAAAGCAGAG CTCCGAGAGT CAGTCTCAGG TTGCTGTTGC TGCACCCAAG
    AAAAAGCGAA AAATGAAGGA ACTAAACAAG AAAGAGGCAG TGGGAGATCT CCTTGATGCG
    TTCAAAGAGG CTGGTGATGG CTCTTCTGAT GTGGATTCTT CCTCTAAGCC TACCAGTGCT
    GCACCTGAAC CAGATCAGAG CCCTTGTGCT CCTCCTGAGG AACAGGATGA AACTTGGGAA
    GAGAAGGAAG ATAAACTGGA TGCAGAGAAT ATTAAACCAG GAGAACATAA ATATAAATAC
    AAAGAAGAGC AATGGAAACC CCTAAACCCG GAGGAAAAGA AGAGTTATGA TCGCGAGTTT
    CTGCTAGGGT TCCAGTTTAT TGTGGCCAGT ATGCAGAAAC CTGCGGGTCT TCCTCAAATT
    ACTGATGTGG TGCTGGATAA GGCAAACAAA ACTCCATTGC GACCATTGGA TCCTACACGT
    CTGACGGGAA TGAACTGTGG GCCAGATTTT ACACCTTCAT TTGCCAACCT TGGTCGGCCT
    GCTGTGGGTA ACCGTGGACC ACCAGCTGGC ATGGGTCCGA GACGGTCCCA GCAGGGTCCA
    CGAAAAGAGC CTCGTAAAAT TATTCAAACC GTATCATTAA ATGAGAATGT ACAACTAAAC
    AAAGCTGAAA AGGCATGGAA ACCGATAAAC AAGAGAGCTG GCGATGAGGA GGACCCAGAA
    AATATTAAGA CACAGGACCT ATTCCGAAGG GTTCGCAGTA TCTTGAATAA GCTGACCCCT
    CAGATGTTCC AGCAGCTGAT GAAGCAGGTC ACTGACCTTA CTATTGATAC CGAAGATCGT
    CTTAAGGGTG TCATTGACCT AATTTTTGAA AAAGCCATTT CAGAACCCAA TTTCTCTGTT
    GCATATGCCA ACATGTGTCG TTGCCTTATG GGGCTTAAAG TTCCAACCAC AGACAAGCCA
    GGGGTTACAG TCAACTTCCG CAAGTTGTTA CTGAATCGGT GCCAGAAGGA GTTTGAGAAG
    GATAAGGATG ATGATGAAGT GTTTGAGAAG AAACAAAAAG AAATGGATGC TGCTGCTTCG
    CCGGAGGAAA AAGCACGTTT GAATGATGAA CTGGTCGAGG CCCGTGACAG TGCACGACGG
    CGATCCTTGG GGAACATTAA GTTCATTGGA GAACTGTTTA AGTTAAAAAT GCTGACTGAA
    GCTATAATGC ACGATTGTGT GGTGAAGCTA TTAAAAAATC ATGATGAGGA ATCTCTGGAG
    TGCCTCTGCC GACTGCTGTC CACCATTGGC AAGGACCTGG ATTTTGAGAG AGCTAAGCCA
    CGTATGGACC AGTACTTCAA CCAGATGGAT AAAATCATTA AGGAGCGGAA GACATCTTCA
    CGAATTAGAT TCATGCTGCA GGATGTGATT GATTTGCGTC TGTGTAATTG GGTGCCTCGT
    CGTGCAGATC AGGGCCCTAA AACTATAGAT CAGATTCATA AAGAAGCAGA AATGGAAAAG
    CAGAGGGAGC AGGTTAAGGT GCAACAACTG ATGTCTAAAC AAGACAAGCG GCGAGAGCCC
    CCAGGACGAC CCACTGGAAG CCGGGGTAAC CAGCCTGCAG ATGATGGCTG GAATACAGTG
    CCCATCAGTA AGGGCAGCCG TCCTATTGAC ACCACTAGGA TCACAAAGAT AACAAAGCTT
    GGTGCTATTG ATTCAAATCA ACTGCTTGCC CCAGGAGGAC GTCTGAGTTG GGGTAAAGGA
    AGCAGCGGAG GAACTGGAGC CAAACCAGCA GAAACAGCTC CAGAATCTAG TCGGCCTGCG
    ACCAGCACCT TAAACAGGTT TTCTGCCTTG CAGCAGTCTT CATCTGCTGA GAGTCAAGAT
    GCTAGACGGC CCTTGCATCG GGGAAGTTCT AGTCGGGAAC GTTCTGAAAA AGATCGTGGA
    GACAGATTTG ATAGAGGTGA AAGGTTTGAT AAGGGAGAAC TGTTTGATAG ATCTGACCGT
    TCAGATCGAG CTCGTCCTCT TCTTACAAAG AGAAGCTATA GCAAGGAAAC TGAGGACCGT
    AGCCGAGAAA GAGAGGCCCA TATACCACAG GAGGCTGTGA GGAAGGTGGC AAGTATGACT
    GAGCAGCGGG ACCGCAGCAA AGAAAGTGTG AAAAAAGAAA TTCCAGCACC GACAGCAGTG
    GTTGCCTCAT CAAAGCCCTC TATGTCTGAG GATGAGCTAG AGAAGAAATC TAAAGCCATC
    ATAGAAGAAT ATTTGCACAT TAATGATATG AAGGAAGCTC TTCAGTGTGT AGTGGAGCTT
    AATAGTTCAA CGCTGCTTTT TATCTTTGTG CGGAATGGGA TTGAGTCAAC ACTAGAGAGA
    AGCACTATTG CCAGAGAGCA TATGGGTCTC CTTTTGTACC AGTTAGTAAA GGCAGGAACT
    CTGTCCAAGG AGCAGTATTA CAAAGGGGTG CTTGAAGTTC TTGAAGTAGC AGAAGATATG
    GAAATAGATA TACCCCACAT CTGGCTGTAT TTAGCAGAGA TAATCAGCCC TGTGCTGCTA
    GAGGGGGGGA TTCCGATGGG GGAGCTGTTC AGAGAGCTGA CCAAACCTTT AGTGCTCATT
    GGCAAAGCTG GAGTCCTTCT GGCAGAAATC CTTGGATTAT TGTGCAAAGG CATGAGTCAT
    AAGAAGGCTG GAGCCCTGTG GAAAGAATCT GGGCTAAGCT GGAAGGAATT CTTGTCCAAG
    GACCAGGATA TTAACAAGTT TATAACTGAC AAGAAACTCG AATACACAAC AGGAGAGGAG
    ACTGAAACAC CCAGTAAGAA AGACCTCACC CCTGCTGAAC TTGATAAAAT TTTGACAAAA
    CTTTTGAAAG AGAACAGCAG TAACCAGAAG ATATTTGACT GGATTGAAGC CAACTTAAAT
    GACCAGCAAA CTTCATCCAA CACCTTTGTT CGAACTTTAA TGAGTACTGT GTGCCACTCT
    GCTATTATTT TTGAGAATCC TTGTAGGGTT GATGTTAATA TTATCCATAC TAGAGCCAAA
    TTTCTTCAGA AATATTTAAG TGACGAGGCC AAGGAACTAC AGGCTCTCTA TGCTCTACAG
    GCACTTGTTG TAAAGCTTGA CCAGCCCCCA AATCTCCTAC GGATGTTTTT TGATGCTCTC
    TATGATGAGG ATGTGATAAA AGAGGATCCA TTCTACAAGT GGGAGTCCAG CAAAGACCCA
    GCTGAACAGC AAGGAAAAGG TGTTGCTCTT AAATCTGTCA CTGCCTTCTT CACATGGCTA
    CGGGAAGCAG AGGATGAGTC TGATAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa10204
    Clone ID Related Accession (Same CDS sequence) XM_018094420.1 , XM_004914398.4 , XM_004914398.3 , XM_002935144.5 , XM_018094419.1 , XM_012963377.3 , XM_002935144.4 , XM_012963377.2
    Accession Version XM_018094419.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4830bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
    CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA
    CAAATGAATA CACAATCTCA GCCTCGACAG GCAGGATTCA GATCAATCCA GCATTATTAT
    CAAAATAGGA CACAGCCTCC CACTAGTGCC ACCAGAGTGC AGAGTGGTTC TTCTGCTCGA
    GCAGTTACTC CAGCCCATGT GTATCAACCA GGCTCCCAAG TTATGATGAT TCCTTCACAG
    ATTTCATACC CTTCCCAGGG ATACTACATT CAGGGACAGG GACGCTCATA TGTGGTGCCC
    ACAAACCAGT ACCCTGTGCA GACTGGGGCT CCAGGTTTCT TCCCCAGTGC AAGTCCCTCA
    GAATATGGCA CATATGCTGG AGCATATTAC CCTGCACAAC AGACGTTCCC TCCTGGAGTG
    CCTGGAGCAC AAGTTATGAT GAATCAGCCA CAGGTGATCC CTCCTAAACG GGAGCGCAAA
    ACGATAAGAA TTAGAGACCC CAACCAAGGA GGCAAAGATA TCACAGAAGA AATTCTCTCA
    GGAGGAAGAA CATCCTCTAC GCCAACGCCT CCACAGTCAA CTGTGGGAAG TTCTGAAATT
    CAAGCTAATG GAGAAAGCGT TCATCATGTT ACCGTTGTTG TGCGCCCAGA TGATGGACCC
    AAGGCCACCC AAGTGCTGAT CAAAGCCTCA GAGAGCCCTT CCCCTCCTCT GCCTCTCTCT
    GAAGCTGAGC CAAGCATAGC AAATGTGTGT GATATTTCAA AAGATAACAC TATCTCTGAG
    TTGACTTCTC ATATAGTAGA GCCCCTAAGT TCAGAAGAGA CAACCATTGA TACTTCTACC
    TCTCAGGTGG ATCTCAAACA CCAGCCAGTG GCTGCTATGG CAGAGCATGA AACTCTTATA
    GAGGAGGCAT CAATACAGGA GGTGGCCTTT GCAGAGGCAC CCATCGCTCA ATCAGAGATA
    CCAAAATCTC TTGCCTCCCA GCCTCTAGCA GAAATCTCTA CCCCTGAACA GATTTCTAAA
    CTTGACGCAC AAGTGCCATC TTTGCAACAA GAGGAGTTAC TTACAGAAAC TGTTGAACTT
    AATTTAAGCA ATCAGGCTCT TCCTTCACAA ATTTCTGAGC CTGAACCTTT TGAACCTGAG
    GCTTCAGCTT CAGATCCTCT ACATGAGGAG CCCACAGAGC CCACAGTTTC AACAGAAGAA
    CCACAAGCTA ATGGACTGTC TGAGGAGACA CCAGTATTAC CTGTACAGCC ACAACCAACA
    TCTGTTTGCC AGACTGAGCC CTCCCTAGAA TCCCCCATCG CTCAGCCTGA GGAGCTGCTT
    CTAACAAATG GAGGTGGACC ACCTGGCCTA CAAGAAAATG ATGCACCTGA TTCACAGGTT
    GAAGCTCATG TTAGCCCAAT CACTGAGCCT GAGGACCAAC CTGTGACAGT CTCTGATAAC
    GTGCTTGTGG AAGAGGATGT AGAAAAAACA TTGCCTGCGG ATACGGAAGT CGTGGCTTCA
    ACCCAAGTTG TAAAGCAGAG CTCCGAGAGT CAGTCTCAGG TTGCTGTTGC TGCACCCAAG
    AAAAAGCGAA AAATGAAGGA ACTAAACAAG AAAGAGGCAG TGGGAGATCT CCTTGATGCG
    TTCAAAGAGG CTGGTGATGG CTCTTCTGAT GTGGATTCTT CCTCTAAGCC TACCAGTGCT
    GCACCTGAAC CAGATCAGAG CCCTTGTGCT CCTCCTGAGG AACAGGATGA AACTTGGGAA
    GAGAAGGAAG ATAAACTGGA TGCAGAGAAT ATTAAACCAG GAGAACATAA ATATAAATAC
    AAAGAAGAGC AATGGAAACC CCTAAACCCG GAGGAAAAGA AGAGTTATGA TCGCGAGTTT
    CTGCTAGGGT TCCAGTTTAT TGTGGCCAGT ATGCAGAAAC CTGCGGGTCT TCCTCAAATT
    ACTGATGTGG TGCTGGATAA GGCAAACAAA ACTCCATTGC GACCATTGGA TCCTACACGT
    CTGACGGGAA TGAACTGTGG GCCAGATTTT ACACCTTCAT TTGCCAACCT TGGTCGGCCT
    GCTGTGGGTA ACCGTGGACC ACCAGCTGGC ATGGGTCCGA GACGGTCCCA GCAGGGTCCA
    CGAAAAGAGC CTCGTAAAAT TATTCAAACC GTATCATTAA ATGAGAATGT ACAACTAAAC
    AAAGCTGAAA AGGCATGGAA ACCGATAAAC AAGAGAGCTG GCGATGAGGA GGACCCAGAA
    AATATTAAGA CACAGGACCT ATTCCGAAGG GTTCGCAGTA TCTTGAATAA GCTGACCCCT
    CAGATGTTCC AGCAGCTGAT GAAGCAGGTC ACTGACCTTA CTATTGATAC CGAAGATCGT
    CTTAAGGGTG TCATTGACCT AATTTTTGAA AAAGCCATTT CAGAACCCAA TTTCTCTGTT
    GCATATGCCA ACATGTGTCG TTGCCTTATG GGGCTTAAAG TTCCAACCAC AGACAAGCCA
    GGGGTTACAG TCAACTTCCG CAAGTTGTTA CTGAATCGGT GCCAGAAGGA GTTTGAGAAG
    GATAAGGATG ATGATGAAGT GTTTGAGAAG AAACAAAAAG AAATGGATGC TGCTGCTTCG
    CCGGAGGAAA AAGCACGTTT GAATGATGAA CTGGTCGAGG CCCGTGACAG TGCACGACGG
    CGATCCTTGG GGAACATTAA GTTCATTGGA GAACTGTTTA AGTTAAAAAT GCTGACTGAA
    GCTATAATGC ACGATTGTGT GGTGAAGCTA TTAAAAAATC ATGATGAGGA ATCTCTGGAG
    TGCCTCTGCC GACTGCTGTC CACCATTGGC AAGGACCTGG ATTTTGAGAG AGCTAAGCCA
    CGTATGGACC AGTACTTCAA CCAGATGGAT AAAATCATTA AGGAGCGGAA GACATCTTCA
    CGAATTAGAT TCATGCTGCA GGATGTGATT GATTTGCGTC TGTGTAATTG GGTGCCTCGT
    CGTGCAGATC AGGGCCCTAA AACTATAGAT CAGATTCATA AAGAAGCAGA AATGGAAAAG
    CAGAGGGAGC AGGTTAAGGT GCAACAACTG ATGTCTAAAC AAGACAAGCG GCGAGAGCCC
    CCAGGACGAC CCACTGGAAG CCGGGGTAAC CAGCCTGCAG ATGATGGCTG GAATACAGTG
    CCCATCAGTA AGGGCAGCCG TCCTATTGAC ACCACTAGGA TCACAAAGAT AACAAAGCTT
    GGTGCTATTG ATTCAAATCA ACTGCTTGCC CCAGGAGGAC GTCTGAGTTG GGGTAAAGGA
    AGCAGCGGAG GAACTGGAGC CAAACCAGCA GAAACAGCTC CAGAATCTAG TCGGCCTGCG
    ACCAGCACCT TAAACAGGTT TTCTGCCTTG CAGCAGTCTT CATCTGCTGA GAGTCAAGAT
    GCTAGACGGC CCTTGCATCG GGGAAGTTCT AGTCGGGAAC GTTCTGAAAA AGATCGTGGA
    GACAGATTTG ATAGAGGTGA AAGGTTTGAT AAGGGAGAAC TGTTTGATAG ATCTGACCGT
    TCAGATCGAG CTCGTCCTCT TCTTACAAAG AGAAGCTATA GCAAGGAAAC TGAGGACCGT
    AGCCGAGAAA GAGAGGCCCA TATACCACAG GAGGCTGTGA GGAAGGTGGC AAGTATGACT
    GAGCAGCGGG ACCGCAGCAA AGAAAGTGTG AAAAAAGAAA TTCCAGCACC GACAGCAGTG
    GTTGCCTCAT CAAAGCCCTC TATGTCTGAG GATGAGCTAG AGAAGAAATC TAAAGCCATC
    ATAGAAGAAT ATTTGCACAT TAATGATATG AAGGAAGCTC TTCAGTGTGT AGTGGAGCTT
    AATAGTTCAA CGCTGCTTTT TATCTTTGTG CGGAATGGGA TTGAGTCAAC ACTAGAGAGA
    AGCACTATTG CCAGAGAGCA TATGGGTCTC CTTTTGTACC AGTTAGTAAA GGCAGGAACT
    CTGTCCAAGG AGCAGTATTA CAAAGGGGTG CTTGAAGTTC TTGAAGTAGC AGAAGATATG
    GAAATAGATA TACCCCACAT CTGGCTGTAT TTAGCAGAGA TAATCAGCCC TGTGCTGCTA
    GAGGGGGGGA TTCCGATGGG GGAGCTGTTC AGAGAGCTGA CCAAACCTTT AGTGCTCATT
    GGCAAAGCTG GAGTCCTTCT GGCAGAAATC CTTGGATTAT TGTGCAAAGG CATGAGTCAT
    AAGAAGGCTG GAGCCCTGTG GAAAGAATCT GGGCTAAGCT GGAAGGAATT CTTGTCCAAG
    GACCAGGATA TTAACAAGTT TATAACTGAC AAGAAACTCG AATACACAAC AGGAGAGGAG
    ACTGAAACAC CCAGTAAGAA AGACCTCACC CCTGCTGAAC TTGATAAAAT TTTGACAAAA
    CTTTTGAAAG AGAACAGCAG TAACCAGAAG ATATTTGACT GGATTGAAGC CAACTTAAAT
    GACCAGCAAA CTTCATCCAA CACCTTTGTT CGAACTTTAA TGAGTACTGT GTGCCACTCT
    GCTATTATTT TTGAGAATCC TTGTAGGGTT GATGTTAATA TTATCCATAC TAGAGCCAAA
    TTTCTTCAGA AATATTTAAG TGACGAGGCC AAGGAACTAC AGGCTCTCTA TGCTCTACAG
    GCACTTGTTG TAAAGCTTGA CCAGCCCCCA AATCTCCTAC GGATGTTTTT TGATGCTCTC
    TATGATGAGG ATGTGATAAA AGAGGATCCA TTCTACAAGT GGGAGTCCAG CAAAGACCCA
    GCTGAACAGC AAGGAAAAGG TGTTGCTCTT AAATCTGTCA CTGCCTTCTT CACATGGCTA
    CGGGAAGCAG AGGATGAGTC TGATAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017949908.1
    CDS226..5055
    Translation

    Target ORF information:

    RefSeq Version XM_018094419.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018094419.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
    CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA
    CAAATGAATA CACAATCTCA GCCTCGACAG GCAGGATTCA GATCAATCCA GCATTATTAT
    CAAAATAGGA CACAGCCTCC CACTAGTGCC ACCAGAGTGC AGAGTGGTTC TTCTGCTCGA
    GCAGTTACTC CAGCCCATGT GTATCAACCA GGCTCCCAAG TTATGATGAT TCCTTCACAG
    ATTTCATACC CTTCCCAGGG ATACTACATT CAGGGACAGG GACGCTCATA TGTGGTGCCC
    ACAAACCAGT ACCCTGTGCA GACTGGGGCT CCAGGTTTCT TCCCCAGTGC AAGTCCCTCA
    GAATATGGCA CATATGCTGG AGCATATTAC CCTGCACAAC AGACGTTCCC TCCTGGAGTG
    CCTGGAGCAC AAGTTATGAT GAATCAGCCA CAGGTGATCC CTCCTAAACG GGAGCGCAAA
    ACGATAAGAA TTAGAGACCC CAACCAAGGA GGCAAAGATA TCACAGAAGA AATTCTCTCA
    GGAGGAAGAA CATCCTCTAC GCCAACGCCT CCACAGTCAA CTGTGGGAAG TTCTGAAATT
    CAAGCTAATG GAGAAAGCGT TCATCATGTT ACCGTTGTTG TGCGCCCAGA TGATGGACCC
    AAGGCCACCC AAGTGCTGAT CAAAGCCTCA GAGAGCCCTT CCCCTCCTCT GCCTCTCTCT
    GAAGCTGAGC CAAGCATAGC AAATGTGTGT GATATTTCAA AAGATAACAC TATCTCTGAG
    TTGACTTCTC ATATAGTAGA GCCCCTAAGT TCAGAAGAGA CAACCATTGA TACTTCTACC
    TCTCAGGTGG ATCTCAAACA CCAGCCAGTG GCTGCTATGG CAGAGCATGA AACTCTTATA
    GAGGAGGCAT CAATACAGGA GGTGGCCTTT GCAGAGGCAC CCATCGCTCA ATCAGAGATA
    CCAAAATCTC TTGCCTCCCA GCCTCTAGCA GAAATCTCTA CCCCTGAACA GATTTCTAAA
    CTTGACGCAC AAGTGCCATC TTTGCAACAA GAGGAGTTAC TTACAGAAAC TGTTGAACTT
    AATTTAAGCA ATCAGGCTCT TCCTTCACAA ATTTCTGAGC CTGAACCTTT TGAACCTGAG
    GCTTCAGCTT CAGATCCTCT ACATGAGGAG CCCACAGAGC CCACAGTTTC AACAGAAGAA
    CCACAAGCTA ATGGACTGTC TGAGGAGACA CCAGTATTAC CTGTACAGCC ACAACCAACA
    TCTGTTTGCC AGACTGAGCC CTCCCTAGAA TCCCCCATCG CTCAGCCTGA GGAGCTGCTT
    CTAACAAATG GAGGTGGACC ACCTGGCCTA CAAGAAAATG ATGCACCTGA TTCACAGGTT
    GAAGCTCATG TTAGCCCAAT CACTGAGCCT GAGGACCAAC CTGTGACAGT CTCTGATAAC
    GTGCTTGTGG AAGAGGATGT AGAAAAAACA TTGCCTGCGG ATACGGAAGT CGTGGCTTCA
    ACCCAAGTTG TAAAGCAGAG CTCCGAGAGT CAGTCTCAGG TTGCTGTTGC TGCACCCAAG
    AAAAAGCGAA AAATGAAGGA ACTAAACAAG AAAGAGGCAG TGGGAGATCT CCTTGATGCG
    TTCAAAGAGG CTGGTGATGG CTCTTCTGAT GTGGATTCTT CCTCTAAGCC TACCAGTGCT
    GCACCTGAAC CAGATCAGAG CCCTTGTGCT CCTCCTGAGG AACAGGATGA AACTTGGGAA
    GAGAAGGAAG ATAAACTGGA TGCAGAGAAT ATTAAACCAG GAGAACATAA ATATAAATAC
    AAAGAAGAGC AATGGAAACC CCTAAACCCG GAGGAAAAGA AGAGTTATGA TCGCGAGTTT
    CTGCTAGGGT TCCAGTTTAT TGTGGCCAGT ATGCAGAAAC CTGCGGGTCT TCCTCAAATT
    ACTGATGTGG TGCTGGATAA GGCAAACAAA ACTCCATTGC GACCATTGGA TCCTACACGT
    CTGACGGGAA TGAACTGTGG GCCAGATTTT ACACCTTCAT TTGCCAACCT TGGTCGGCCT
    GCTGTGGGTA ACCGTGGACC ACCAGCTGGC ATGGGTCCGA GACGGTCCCA GCAGGGTCCA
    CGAAAAGAGC CTCGTAAAAT TATTCAAACC GTATCATTAA ATGAGAATGT ACAACTAAAC
    AAAGCTGAAA AGGCATGGAA ACCGATAAAC AAGAGAGCTG GCGATGAGGA GGACCCAGAA
    AATATTAAGA CACAGGACCT ATTCCGAAGG GTTCGCAGTA TCTTGAATAA GCTGACCCCT
    CAGATGTTCC AGCAGCTGAT GAAGCAGGTC ACTGACCTTA CTATTGATAC CGAAGATCGT
    CTTAAGGGTG TCATTGACCT AATTTTTGAA AAAGCCATTT CAGAACCCAA TTTCTCTGTT
    GCATATGCCA ACATGTGTCG TTGCCTTATG GGGCTTAAAG TTCCAACCAC AGACAAGCCA
    GGGGTTACAG TCAACTTCCG CAAGTTGTTA CTGAATCGGT GCCAGAAGGA GTTTGAGAAG
    GATAAGGATG ATGATGAAGT GTTTGAGAAG AAACAAAAAG AAATGGATGC TGCTGCTTCG
    CCGGAGGAAA AAGCACGTTT GAATGATGAA CTGGTCGAGG CCCGTGACAG TGCACGACGG
    CGATCCTTGG GGAACATTAA GTTCATTGGA GAACTGTTTA AGTTAAAAAT GCTGACTGAA
    GCTATAATGC ACGATTGTGT GGTGAAGCTA TTAAAAAATC ATGATGAGGA ATCTCTGGAG
    TGCCTCTGCC GACTGCTGTC CACCATTGGC AAGGACCTGG ATTTTGAGAG AGCTAAGCCA
    CGTATGGACC AGTACTTCAA CCAGATGGAT AAAATCATTA AGGAGCGGAA GACATCTTCA
    CGAATTAGAT TCATGCTGCA GGATGTGATT GATTTGCGTC TGTGTAATTG GGTGCCTCGT
    CGTGCAGATC AGGGCCCTAA AACTATAGAT CAGATTCATA AAGAAGCAGA AATGGAAAAG
    CAGAGGGAGC AGGTTAAGGT GCAACAACTG ATGTCTAAAC AAGACAAGCG GCGAGAGCCC
    CCAGGACGAC CCACTGGAAG CCGGGGTAAC CAGCCTGCAG ATGATGGCTG GAATACAGTG
    CCCATCAGTA AGGGCAGCCG TCCTATTGAC ACCACTAGGA TCACAAAGAT AACAAAGCTT
    GGTGCTATTG ATTCAAATCA ACTGCTTGCC CCAGGAGGAC GTCTGAGTTG GGGTAAAGGA
    AGCAGCGGAG GAACTGGAGC CAAACCAGCA GAAACAGCTC CAGAATCTAG TCGGCCTGCG
    ACCAGCACCT TAAACAGGTT TTCTGCCTTG CAGCAGTCTT CATCTGCTGA GAGTCAAGAT
    GCTAGACGGC CCTTGCATCG GGGAAGTTCT AGTCGGGAAC GTTCTGAAAA AGATCGTGGA
    GACAGATTTG ATAGAGGTGA AAGGTTTGAT AAGGGAGAAC TGTTTGATAG ATCTGACCGT
    TCAGATCGAG CTCGTCCTCT TCTTACAAAG AGAAGCTATA GCAAGGAAAC TGAGGACCGT
    AGCCGAGAAA GAGAGGCCCA TATACCACAG GAGGCTGTGA GGAAGGTGGC AAGTATGACT
    GAGCAGCGGG ACCGCAGCAA AGAAAGTGTG AAAAAAGAAA TTCCAGCACC GACAGCAGTG
    GTTGCCTCAT CAAAGCCCTC TATGTCTGAG GATGAGCTAG AGAAGAAATC TAAAGCCATC
    ATAGAAGAAT ATTTGCACAT TAATGATATG AAGGAAGCTC TTCAGTGTGT AGTGGAGCTT
    AATAGTTCAA CGCTGCTTTT TATCTTTGTG CGGAATGGGA TTGAGTCAAC ACTAGAGAGA
    AGCACTATTG CCAGAGAGCA TATGGGTCTC CTTTTGTACC AGTTAGTAAA GGCAGGAACT
    CTGTCCAAGG AGCAGTATTA CAAAGGGGTG CTTGAAGTTC TTGAAGTAGC AGAAGATATG
    GAAATAGATA TACCCCACAT CTGGCTGTAT TTAGCAGAGA TAATCAGCCC TGTGCTGCTA
    GAGGGGGGGA TTCCGATGGG GGAGCTGTTC AGAGAGCTGA CCAAACCTTT AGTGCTCATT
    GGCAAAGCTG GAGTCCTTCT GGCAGAAATC CTTGGATTAT TGTGCAAAGG CATGAGTCAT
    AAGAAGGCTG GAGCCCTGTG GAAAGAATCT GGGCTAAGCT GGAAGGAATT CTTGTCCAAG
    GACCAGGATA TTAACAAGTT TATAACTGAC AAGAAACTCG AATACACAAC AGGAGAGGAG
    ACTGAAACAC CCAGTAAGAA AGACCTCACC CCTGCTGAAC TTGATAAAAT TTTGACAAAA
    CTTTTGAAAG AGAACAGCAG TAACCAGAAG ATATTTGACT GGATTGAAGC CAACTTAAAT
    GACCAGCAAA CTTCATCCAA CACCTTTGTT CGAACTTTAA TGAGTACTGT GTGCCACTCT
    GCTATTATTT TTGAGAATCC TTGTAGGGTT GATGTTAATA TTATCCATAC TAGAGCCAAA
    TTTCTTCAGA AATATTTAAG TGACGAGGCC AAGGAACTAC AGGCTCTCTA TGCTCTACAG
    GCACTTGTTG TAAAGCTTGA CCAGCCCCCA AATCTCCTAC GGATGTTTTT TGATGCTCTC
    TATGATGAGG ATGTGATAAA AGAGGATCCA TTCTACAAGT GGGAGTCCAG CAAAGACCCA
    GCTGAACAGC AAGGAAAAGG TGTTGCTCTT AAATCTGTCA CTGCCTTCTT CACATGGCTA
    CGGGAAGCAG AGGATGAGTC TGATAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa10204
    Clone ID Related Accession (Same CDS sequence) XM_018094420.1 , XM_004914398.4 , XM_004914398.3 , XM_002935144.5 , XM_018094419.1 , XM_012963377.3 , XM_002935144.4 , XM_012963377.2
    Accession Version XM_002935144.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4830bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002935144.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
    CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA
    CAAATGAATA CACAATCTCA GCCTCGACAG GCAGGATTCA GATCAATCCA GCATTATTAT
    CAAAATAGGA CACAGCCTCC CACTAGTGCC ACCAGAGTGC AGAGTGGTTC TTCTGCTCGA
    GCAGTTACTC CAGCCCATGT GTATCAACCA GGCTCCCAAG TTATGATGAT TCCTTCACAG
    ATTTCATACC CTTCCCAGGG ATACTACATT CAGGGACAGG GACGCTCATA TGTGGTGCCC
    ACAAACCAGT ACCCTGTGCA GACTGGGGCT CCAGGTTTCT TCCCCAGTGC AAGTCCCTCA
    GAATATGGCA CATATGCTGG AGCATATTAC CCTGCACAAC AGACGTTCCC TCCTGGAGTG
    CCTGGAGCAC AAGTTATGAT GAATCAGCCA CAGGTGATCC CTCCTAAACG GGAGCGCAAA
    ACGATAAGAA TTAGAGACCC CAACCAAGGA GGCAAAGATA TCACAGAAGA AATTCTCTCA
    GGAGGAAGAA CATCCTCTAC GCCAACGCCT CCACAGTCAA CTGTGGGAAG TTCTGAAATT
    CAAGCTAATG GAGAAAGCGT TCATCATGTT ACCGTTGTTG TGCGCCCAGA TGATGGACCC
    AAGGCCACCC AAGTGCTGAT CAAAGCCTCA GAGAGCCCTT CCCCTCCTCT GCCTCTCTCT
    GAAGCTGAGC CAAGCATAGC AAATGTGTGT GATATTTCAA AAGATAACAC TATCTCTGAG
    TTGACTTCTC ATATAGTAGA GCCCCTAAGT TCAGAAGAGA CAACCATTGA TACTTCTACC
    TCTCAGGTGG ATCTCAAACA CCAGCCAGTG GCTGCTATGG CAGAGCATGA AACTCTTATA
    GAGGAGGCAT CAATACAGGA GGTGGCCTTT GCAGAGGCAC CCATCGCTCA ATCAGAGATA
    CCAAAATCTC TTGCCTCCCA GCCTCTAGCA GAAATCTCTA CCCCTGAACA GATTTCTAAA
    CTTGACGCAC AAGTGCCATC TTTGCAACAA GAGGAGTTAC TTACAGAAAC TGTTGAACTT
    AATTTAAGCA ATCAGGCTCT TCCTTCACAA ATTTCTGAGC CTGAACCTTT TGAACCTGAG
    GCTTCAGCTT CAGATCCTCT ACATGAGGAG CCCACAGAGC CCACAGTTTC AACAGAAGAA
    CCACAAGCTA ATGGACTGTC TGAGGAGACA CCAGTATTAC CTGTACAGCC ACAACCAACA
    TCTGTTTGCC AGACTGAGCC CTCCCTAGAA TCCCCCATCG CTCAGCCTGA GGAGCTGCTT
    CTAACAAATG GAGGTGGACC ACCTGGCCTA CAAGAAAATG ATGCACCTGA TTCACAGGTT
    GAAGCTCATG TTAGCCCAAT CACTGAGCCT GAGGACCAAC CTGTGACAGT CTCTGATAAC
    GTGCTTGTGG AAGAGGATGT AGAAAAAACA TTGCCTGCGG ATACGGAAGT CGTGGCTTCA
    ACCCAAGTTG TAAAGCAGAG CTCCGAGAGT CAGTCTCAGG TTGCTGTTGC TGCACCCAAG
    AAAAAGCGAA AAATGAAGGA ACTAAACAAG AAAGAGGCAG TGGGAGATCT CCTTGATGCG
    TTCAAAGAGG CTGGTGATGG CTCTTCTGAT GTGGATTCTT CCTCTAAGCC TACCAGTGCT
    GCACCTGAAC CAGATCAGAG CCCTTGTGCT CCTCCTGAGG AACAGGATGA AACTTGGGAA
    GAGAAGGAAG ATAAACTGGA TGCAGAGAAT ATTAAACCAG GAGAACATAA ATATAAATAC
    AAAGAAGAGC AATGGAAACC CCTAAACCCG GAGGAAAAGA AGAGTTATGA TCGCGAGTTT
    CTGCTAGGGT TCCAGTTTAT TGTGGCCAGT ATGCAGAAAC CTGCGGGTCT TCCTCAAATT
    ACTGATGTGG TGCTGGATAA GGCAAACAAA ACTCCATTGC GACCATTGGA TCCTACACGT
    CTGACGGGAA TGAACTGTGG GCCAGATTTT ACACCTTCAT TTGCCAACCT TGGTCGGCCT
    GCTGTGGGTA ACCGTGGACC ACCAGCTGGC ATGGGTCCGA GACGGTCCCA GCAGGGTCCA
    CGAAAAGAGC CTCGTAAAAT TATTCAAACC GTATCATTAA ATGAGAATGT ACAACTAAAC
    AAAGCTGAAA AGGCATGGAA ACCGATAAAC AAGAGAGCTG GCGATGAGGA GGACCCAGAA
    AATATTAAGA CACAGGACCT ATTCCGAAGG GTTCGCAGTA TCTTGAATAA GCTGACCCCT
    CAGATGTTCC AGCAGCTGAT GAAGCAGGTC ACTGACCTTA CTATTGATAC CGAAGATCGT
    CTTAAGGGTG TCATTGACCT AATTTTTGAA AAAGCCATTT CAGAACCCAA TTTCTCTGTT
    GCATATGCCA ACATGTGTCG TTGCCTTATG GGGCTTAAAG TTCCAACCAC AGACAAGCCA
    GGGGTTACAG TCAACTTCCG CAAGTTGTTA CTGAATCGGT GCCAGAAGGA GTTTGAGAAG
    GATAAGGATG ATGATGAAGT GTTTGAGAAG AAACAAAAAG AAATGGATGC TGCTGCTTCG
    CCGGAGGAAA AAGCACGTTT GAATGATGAA CTGGTCGAGG CCCGTGACAG TGCACGACGG
    CGATCCTTGG GGAACATTAA GTTCATTGGA GAACTGTTTA AGTTAAAAAT GCTGACTGAA
    GCTATAATGC ACGATTGTGT GGTGAAGCTA TTAAAAAATC ATGATGAGGA ATCTCTGGAG
    TGCCTCTGCC GACTGCTGTC CACCATTGGC AAGGACCTGG ATTTTGAGAG AGCTAAGCCA
    CGTATGGACC AGTACTTCAA CCAGATGGAT AAAATCATTA AGGAGCGGAA GACATCTTCA
    CGAATTAGAT TCATGCTGCA GGATGTGATT GATTTGCGTC TGTGTAATTG GGTGCCTCGT
    CGTGCAGATC AGGGCCCTAA AACTATAGAT CAGATTCATA AAGAAGCAGA AATGGAAAAG
    CAGAGGGAGC AGGTTAAGGT GCAACAACTG ATGTCTAAAC AAGACAAGCG GCGAGAGCCC
    CCAGGACGAC CCACTGGAAG CCGGGGTAAC CAGCCTGCAG ATGATGGCTG GAATACAGTG
    CCCATCAGTA AGGGCAGCCG TCCTATTGAC ACCACTAGGA TCACAAAGAT AACAAAGCTT
    GGTGCTATTG ATTCAAATCA ACTGCTTGCC CCAGGAGGAC GTCTGAGTTG GGGTAAAGGA
    AGCAGCGGAG GAACTGGAGC CAAACCAGCA GAAACAGCTC CAGAATCTAG TCGGCCTGCG
    ACCAGCACCT TAAACAGGTT TTCTGCCTTG CAGCAGTCTT CATCTGCTGA GAGTCAAGAT
    GCTAGACGGC CCTTGCATCG GGGAAGTTCT AGTCGGGAAC GTTCTGAAAA AGATCGTGGA
    GACAGATTTG ATAGAGGTGA AAGGTTTGAT AAGGGAGAAC TGTTTGATAG ATCTGACCGT
    TCAGATCGAG CTCGTCCTCT TCTTACAAAG AGAAGCTATA GCAAGGAAAC TGAGGACCGT
    AGCCGAGAAA GAGAGGCCCA TATACCACAG GAGGCTGTGA GGAAGGTGGC AAGTATGACT
    GAGCAGCGGG ACCGCAGCAA AGAAAGTGTG AAAAAAGAAA TTCCAGCACC GACAGCAGTG
    GTTGCCTCAT CAAAGCCCTC TATGTCTGAG GATGAGCTAG AGAAGAAATC TAAAGCCATC
    ATAGAAGAAT ATTTGCACAT TAATGATATG AAGGAAGCTC TTCAGTGTGT AGTGGAGCTT
    AATAGTTCAA CGCTGCTTTT TATCTTTGTG CGGAATGGGA TTGAGTCAAC ACTAGAGAGA
    AGCACTATTG CCAGAGAGCA TATGGGTCTC CTTTTGTACC AGTTAGTAAA GGCAGGAACT
    CTGTCCAAGG AGCAGTATTA CAAAGGGGTG CTTGAAGTTC TTGAAGTAGC AGAAGATATG
    GAAATAGATA TACCCCACAT CTGGCTGTAT TTAGCAGAGA TAATCAGCCC TGTGCTGCTA
    GAGGGGGGGA TTCCGATGGG GGAGCTGTTC AGAGAGCTGA CCAAACCTTT AGTGCTCATT
    GGCAAAGCTG GAGTCCTTCT GGCAGAAATC CTTGGATTAT TGTGCAAAGG CATGAGTCAT
    AAGAAGGCTG GAGCCCTGTG GAAAGAATCT GGGCTAAGCT GGAAGGAATT CTTGTCCAAG
    GACCAGGATA TTAACAAGTT TATAACTGAC AAGAAACTCG AATACACAAC AGGAGAGGAG
    ACTGAAACAC CCAGTAAGAA AGACCTCACC CCTGCTGAAC TTGATAAAAT TTTGACAAAA
    CTTTTGAAAG AGAACAGCAG TAACCAGAAG ATATTTGACT GGATTGAAGC CAACTTAAAT
    GACCAGCAAA CTTCATCCAA CACCTTTGTT CGAACTTTAA TGAGTACTGT GTGCCACTCT
    GCTATTATTT TTGAGAATCC TTGTAGGGTT GATGTTAATA TTATCCATAC TAGAGCCAAA
    TTTCTTCAGA AATATTTAAG TGACGAGGCC AAGGAACTAC AGGCTCTCTA TGCTCTACAG
    GCACTTGTTG TAAAGCTTGA CCAGCCCCCA AATCTCCTAC GGATGTTTTT TGATGCTCTC
    TATGATGAGG ATGTGATAAA AGAGGATCCA TTCTACAAGT GGGAGTCCAG CAAAGACCCA
    GCTGAACAGC AAGGAAAAGG TGTTGCTCTT AAATCTGTCA CTGCCTTCTT CACATGGCTA
    CGGGAAGCAG AGGATGAGTC TGATAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002935190.1
    CDS216..5045
    Translation

    Target ORF information:

    RefSeq Version XM_002935144.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002935144.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
    CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA
    CAAATGAATA CACAATCTCA GCCTCGACAG GCAGGATTCA GATCAATCCA GCATTATTAT
    CAAAATAGGA CACAGCCTCC CACTAGTGCC ACCAGAGTGC AGAGTGGTTC TTCTGCTCGA
    GCAGTTACTC CAGCCCATGT GTATCAACCA GGCTCCCAAG TTATGATGAT TCCTTCACAG
    ATTTCATACC CTTCCCAGGG ATACTACATT CAGGGACAGG GACGCTCATA TGTGGTGCCC
    ACAAACCAGT ACCCTGTGCA GACTGGGGCT CCAGGTTTCT TCCCCAGTGC AAGTCCCTCA
    GAATATGGCA CATATGCTGG AGCATATTAC CCTGCACAAC AGACGTTCCC TCCTGGAGTG
    CCTGGAGCAC AAGTTATGAT GAATCAGCCA CAGGTGATCC CTCCTAAACG GGAGCGCAAA
    ACGATAAGAA TTAGAGACCC CAACCAAGGA GGCAAAGATA TCACAGAAGA AATTCTCTCA
    GGAGGAAGAA CATCCTCTAC GCCAACGCCT CCACAGTCAA CTGTGGGAAG TTCTGAAATT
    CAAGCTAATG GAGAAAGCGT TCATCATGTT ACCGTTGTTG TGCGCCCAGA TGATGGACCC
    AAGGCCACCC AAGTGCTGAT CAAAGCCTCA GAGAGCCCTT CCCCTCCTCT GCCTCTCTCT
    GAAGCTGAGC CAAGCATAGC AAATGTGTGT GATATTTCAA AAGATAACAC TATCTCTGAG
    TTGACTTCTC ATATAGTAGA GCCCCTAAGT TCAGAAGAGA CAACCATTGA TACTTCTACC
    TCTCAGGTGG ATCTCAAACA CCAGCCAGTG GCTGCTATGG CAGAGCATGA AACTCTTATA
    GAGGAGGCAT CAATACAGGA GGTGGCCTTT GCAGAGGCAC CCATCGCTCA ATCAGAGATA
    CCAAAATCTC TTGCCTCCCA GCCTCTAGCA GAAATCTCTA CCCCTGAACA GATTTCTAAA
    CTTGACGCAC AAGTGCCATC TTTGCAACAA GAGGAGTTAC TTACAGAAAC TGTTGAACTT
    AATTTAAGCA ATCAGGCTCT TCCTTCACAA ATTTCTGAGC CTGAACCTTT TGAACCTGAG
    GCTTCAGCTT CAGATCCTCT ACATGAGGAG CCCACAGAGC CCACAGTTTC AACAGAAGAA
    CCACAAGCTA ATGGACTGTC TGAGGAGACA CCAGTATTAC CTGTACAGCC ACAACCAACA
    TCTGTTTGCC AGACTGAGCC CTCCCTAGAA TCCCCCATCG CTCAGCCTGA GGAGCTGCTT
    CTAACAAATG GAGGTGGACC ACCTGGCCTA CAAGAAAATG ATGCACCTGA TTCACAGGTT
    GAAGCTCATG TTAGCCCAAT CACTGAGCCT GAGGACCAAC CTGTGACAGT CTCTGATAAC
    GTGCTTGTGG AAGAGGATGT AGAAAAAACA TTGCCTGCGG ATACGGAAGT CGTGGCTTCA
    ACCCAAGTTG TAAAGCAGAG CTCCGAGAGT CAGTCTCAGG TTGCTGTTGC TGCACCCAAG
    AAAAAGCGAA AAATGAAGGA ACTAAACAAG AAAGAGGCAG TGGGAGATCT CCTTGATGCG
    TTCAAAGAGG CTGGTGATGG CTCTTCTGAT GTGGATTCTT CCTCTAAGCC TACCAGTGCT
    GCACCTGAAC CAGATCAGAG CCCTTGTGCT CCTCCTGAGG AACAGGATGA AACTTGGGAA
    GAGAAGGAAG ATAAACTGGA TGCAGAGAAT ATTAAACCAG GAGAACATAA ATATAAATAC
    AAAGAAGAGC AATGGAAACC CCTAAACCCG GAGGAAAAGA AGAGTTATGA TCGCGAGTTT
    CTGCTAGGGT TCCAGTTTAT TGTGGCCAGT ATGCAGAAAC CTGCGGGTCT TCCTCAAATT
    ACTGATGTGG TGCTGGATAA GGCAAACAAA ACTCCATTGC GACCATTGGA TCCTACACGT
    CTGACGGGAA TGAACTGTGG GCCAGATTTT ACACCTTCAT TTGCCAACCT TGGTCGGCCT
    GCTGTGGGTA ACCGTGGACC ACCAGCTGGC ATGGGTCCGA GACGGTCCCA GCAGGGTCCA
    CGAAAAGAGC CTCGTAAAAT TATTCAAACC GTATCATTAA ATGAGAATGT ACAACTAAAC
    AAAGCTGAAA AGGCATGGAA ACCGATAAAC AAGAGAGCTG GCGATGAGGA GGACCCAGAA
    AATATTAAGA CACAGGACCT ATTCCGAAGG GTTCGCAGTA TCTTGAATAA GCTGACCCCT
    CAGATGTTCC AGCAGCTGAT GAAGCAGGTC ACTGACCTTA CTATTGATAC CGAAGATCGT
    CTTAAGGGTG TCATTGACCT AATTTTTGAA AAAGCCATTT CAGAACCCAA TTTCTCTGTT
    GCATATGCCA ACATGTGTCG TTGCCTTATG GGGCTTAAAG TTCCAACCAC AGACAAGCCA
    GGGGTTACAG TCAACTTCCG CAAGTTGTTA CTGAATCGGT GCCAGAAGGA GTTTGAGAAG
    GATAAGGATG ATGATGAAGT GTTTGAGAAG AAACAAAAAG AAATGGATGC TGCTGCTTCG
    CCGGAGGAAA AAGCACGTTT GAATGATGAA CTGGTCGAGG CCCGTGACAG TGCACGACGG
    CGATCCTTGG GGAACATTAA GTTCATTGGA GAACTGTTTA AGTTAAAAAT GCTGACTGAA
    GCTATAATGC ACGATTGTGT GGTGAAGCTA TTAAAAAATC ATGATGAGGA ATCTCTGGAG
    TGCCTCTGCC GACTGCTGTC CACCATTGGC AAGGACCTGG ATTTTGAGAG AGCTAAGCCA
    CGTATGGACC AGTACTTCAA CCAGATGGAT AAAATCATTA AGGAGCGGAA GACATCTTCA
    CGAATTAGAT TCATGCTGCA GGATGTGATT GATTTGCGTC TGTGTAATTG GGTGCCTCGT
    CGTGCAGATC AGGGCCCTAA AACTATAGAT CAGATTCATA AAGAAGCAGA AATGGAAAAG
    CAGAGGGAGC AGGTTAAGGT GCAACAACTG ATGTCTAAAC AAGACAAGCG GCGAGAGCCC
    CCAGGACGAC CCACTGGAAG CCGGGGTAAC CAGCCTGCAG ATGATGGCTG GAATACAGTG
    CCCATCAGTA AGGGCAGCCG TCCTATTGAC ACCACTAGGA TCACAAAGAT AACAAAGCTT
    GGTGCTATTG ATTCAAATCA ACTGCTTGCC CCAGGAGGAC GTCTGAGTTG GGGTAAAGGA
    AGCAGCGGAG GAACTGGAGC CAAACCAGCA GAAACAGCTC CAGAATCTAG TCGGCCTGCG
    ACCAGCACCT TAAACAGGTT TTCTGCCTTG CAGCAGTCTT CATCTGCTGA GAGTCAAGAT
    GCTAGACGGC CCTTGCATCG GGGAAGTTCT AGTCGGGAAC GTTCTGAAAA AGATCGTGGA
    GACAGATTTG ATAGAGGTGA AAGGTTTGAT AAGGGAGAAC TGTTTGATAG ATCTGACCGT
    TCAGATCGAG CTCGTCCTCT TCTTACAAAG AGAAGCTATA GCAAGGAAAC TGAGGACCGT
    AGCCGAGAAA GAGAGGCCCA TATACCACAG GAGGCTGTGA GGAAGGTGGC AAGTATGACT
    GAGCAGCGGG ACCGCAGCAA AGAAAGTGTG AAAAAAGAAA TTCCAGCACC GACAGCAGTG
    GTTGCCTCAT CAAAGCCCTC TATGTCTGAG GATGAGCTAG AGAAGAAATC TAAAGCCATC
    ATAGAAGAAT ATTTGCACAT TAATGATATG AAGGAAGCTC TTCAGTGTGT AGTGGAGCTT
    AATAGTTCAA CGCTGCTTTT TATCTTTGTG CGGAATGGGA TTGAGTCAAC ACTAGAGAGA
    AGCACTATTG CCAGAGAGCA TATGGGTCTC CTTTTGTACC AGTTAGTAAA GGCAGGAACT
    CTGTCCAAGG AGCAGTATTA CAAAGGGGTG CTTGAAGTTC TTGAAGTAGC AGAAGATATG
    GAAATAGATA TACCCCACAT CTGGCTGTAT TTAGCAGAGA TAATCAGCCC TGTGCTGCTA
    GAGGGGGGGA TTCCGATGGG GGAGCTGTTC AGAGAGCTGA CCAAACCTTT AGTGCTCATT
    GGCAAAGCTG GAGTCCTTCT GGCAGAAATC CTTGGATTAT TGTGCAAAGG CATGAGTCAT
    AAGAAGGCTG GAGCCCTGTG GAAAGAATCT GGGCTAAGCT GGAAGGAATT CTTGTCCAAG
    GACCAGGATA TTAACAAGTT TATAACTGAC AAGAAACTCG AATACACAAC AGGAGAGGAG
    ACTGAAACAC CCAGTAAGAA AGACCTCACC CCTGCTGAAC TTGATAAAAT TTTGACAAAA
    CTTTTGAAAG AGAACAGCAG TAACCAGAAG ATATTTGACT GGATTGAAGC CAACTTAAAT
    GACCAGCAAA CTTCATCCAA CACCTTTGTT CGAACTTTAA TGAGTACTGT GTGCCACTCT
    GCTATTATTT TTGAGAATCC TTGTAGGGTT GATGTTAATA TTATCCATAC TAGAGCCAAA
    TTTCTTCAGA AATATTTAAG TGACGAGGCC AAGGAACTAC AGGCTCTCTA TGCTCTACAG
    GCACTTGTTG TAAAGCTTGA CCAGCCCCCA AATCTCCTAC GGATGTTTTT TGATGCTCTC
    TATGATGAGG ATGTGATAAA AGAGGATCCA TTCTACAAGT GGGAGTCCAG CAAAGACCCA
    GCTGAACAGC AAGGAAAAGG TGTTGCTCTT AAATCTGTCA CTGCCTTCTT CACATGGCTA
    CGGGAAGCAG AGGATGAGTC TGATAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa10204
    Clone ID Related Accession (Same CDS sequence) XM_018094420.1 , XM_004914398.4 , XM_004914398.3 , XM_002935144.5 , XM_018094419.1 , XM_012963377.3 , XM_002935144.4 , XM_012963377.2
    Accession Version XM_012963377.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4830bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963377.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
    CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA
    CAAATGAATA CACAATCTCA GCCTCGACAG GCAGGATTCA GATCAATCCA GCATTATTAT
    CAAAATAGGA CACAGCCTCC CACTAGTGCC ACCAGAGTGC AGAGTGGTTC TTCTGCTCGA
    GCAGTTACTC CAGCCCATGT GTATCAACCA GGCTCCCAAG TTATGATGAT TCCTTCACAG
    ATTTCATACC CTTCCCAGGG ATACTACATT CAGGGACAGG GACGCTCATA TGTGGTGCCC
    ACAAACCAGT ACCCTGTGCA GACTGGGGCT CCAGGTTTCT TCCCCAGTGC AAGTCCCTCA
    GAATATGGCA CATATGCTGG AGCATATTAC CCTGCACAAC AGACGTTCCC TCCTGGAGTG
    CCTGGAGCAC AAGTTATGAT GAATCAGCCA CAGGTGATCC CTCCTAAACG GGAGCGCAAA
    ACGATAAGAA TTAGAGACCC CAACCAAGGA GGCAAAGATA TCACAGAAGA AATTCTCTCA
    GGAGGAAGAA CATCCTCTAC GCCAACGCCT CCACAGTCAA CTGTGGGAAG TTCTGAAATT
    CAAGCTAATG GAGAAAGCGT TCATCATGTT ACCGTTGTTG TGCGCCCAGA TGATGGACCC
    AAGGCCACCC AAGTGCTGAT CAAAGCCTCA GAGAGCCCTT CCCCTCCTCT GCCTCTCTCT
    GAAGCTGAGC CAAGCATAGC AAATGTGTGT GATATTTCAA AAGATAACAC TATCTCTGAG
    TTGACTTCTC ATATAGTAGA GCCCCTAAGT TCAGAAGAGA CAACCATTGA TACTTCTACC
    TCTCAGGTGG ATCTCAAACA CCAGCCAGTG GCTGCTATGG CAGAGCATGA AACTCTTATA
    GAGGAGGCAT CAATACAGGA GGTGGCCTTT GCAGAGGCAC CCATCGCTCA ATCAGAGATA
    CCAAAATCTC TTGCCTCCCA GCCTCTAGCA GAAATCTCTA CCCCTGAACA GATTTCTAAA
    CTTGACGCAC AAGTGCCATC TTTGCAACAA GAGGAGTTAC TTACAGAAAC TGTTGAACTT
    AATTTAAGCA ATCAGGCTCT TCCTTCACAA ATTTCTGAGC CTGAACCTTT TGAACCTGAG
    GCTTCAGCTT CAGATCCTCT ACATGAGGAG CCCACAGAGC CCACAGTTTC AACAGAAGAA
    CCACAAGCTA ATGGACTGTC TGAGGAGACA CCAGTATTAC CTGTACAGCC ACAACCAACA
    TCTGTTTGCC AGACTGAGCC CTCCCTAGAA TCCCCCATCG CTCAGCCTGA GGAGCTGCTT
    CTAACAAATG GAGGTGGACC ACCTGGCCTA CAAGAAAATG ATGCACCTGA TTCACAGGTT
    GAAGCTCATG TTAGCCCAAT CACTGAGCCT GAGGACCAAC CTGTGACAGT CTCTGATAAC
    GTGCTTGTGG AAGAGGATGT AGAAAAAACA TTGCCTGCGG ATACGGAAGT CGTGGCTTCA
    ACCCAAGTTG TAAAGCAGAG CTCCGAGAGT CAGTCTCAGG TTGCTGTTGC TGCACCCAAG
    AAAAAGCGAA AAATGAAGGA ACTAAACAAG AAAGAGGCAG TGGGAGATCT CCTTGATGCG
    TTCAAAGAGG CTGGTGATGG CTCTTCTGAT GTGGATTCTT CCTCTAAGCC TACCAGTGCT
    GCACCTGAAC CAGATCAGAG CCCTTGTGCT CCTCCTGAGG AACAGGATGA AACTTGGGAA
    GAGAAGGAAG ATAAACTGGA TGCAGAGAAT ATTAAACCAG GAGAACATAA ATATAAATAC
    AAAGAAGAGC AATGGAAACC CCTAAACCCG GAGGAAAAGA AGAGTTATGA TCGCGAGTTT
    CTGCTAGGGT TCCAGTTTAT TGTGGCCAGT ATGCAGAAAC CTGCGGGTCT TCCTCAAATT
    ACTGATGTGG TGCTGGATAA GGCAAACAAA ACTCCATTGC GACCATTGGA TCCTACACGT
    CTGACGGGAA TGAACTGTGG GCCAGATTTT ACACCTTCAT TTGCCAACCT TGGTCGGCCT
    GCTGTGGGTA ACCGTGGACC ACCAGCTGGC ATGGGTCCGA GACGGTCCCA GCAGGGTCCA
    CGAAAAGAGC CTCGTAAAAT TATTCAAACC GTATCATTAA ATGAGAATGT ACAACTAAAC
    AAAGCTGAAA AGGCATGGAA ACCGATAAAC AAGAGAGCTG GCGATGAGGA GGACCCAGAA
    AATATTAAGA CACAGGACCT ATTCCGAAGG GTTCGCAGTA TCTTGAATAA GCTGACCCCT
    CAGATGTTCC AGCAGCTGAT GAAGCAGGTC ACTGACCTTA CTATTGATAC CGAAGATCGT
    CTTAAGGGTG TCATTGACCT AATTTTTGAA AAAGCCATTT CAGAACCCAA TTTCTCTGTT
    GCATATGCCA ACATGTGTCG TTGCCTTATG GGGCTTAAAG TTCCAACCAC AGACAAGCCA
    GGGGTTACAG TCAACTTCCG CAAGTTGTTA CTGAATCGGT GCCAGAAGGA GTTTGAGAAG
    GATAAGGATG ATGATGAAGT GTTTGAGAAG AAACAAAAAG AAATGGATGC TGCTGCTTCG
    CCGGAGGAAA AAGCACGTTT GAATGATGAA CTGGTCGAGG CCCGTGACAG TGCACGACGG
    CGATCCTTGG GGAACATTAA GTTCATTGGA GAACTGTTTA AGTTAAAAAT GCTGACTGAA
    GCTATAATGC ACGATTGTGT GGTGAAGCTA TTAAAAAATC ATGATGAGGA ATCTCTGGAG
    TGCCTCTGCC GACTGCTGTC CACCATTGGC AAGGACCTGG ATTTTGAGAG AGCTAAGCCA
    CGTATGGACC AGTACTTCAA CCAGATGGAT AAAATCATTA AGGAGCGGAA GACATCTTCA
    CGAATTAGAT TCATGCTGCA GGATGTGATT GATTTGCGTC TGTGTAATTG GGTGCCTCGT
    CGTGCAGATC AGGGCCCTAA AACTATAGAT CAGATTCATA AAGAAGCAGA AATGGAAAAG
    CAGAGGGAGC AGGTTAAGGT GCAACAACTG ATGTCTAAAC AAGACAAGCG GCGAGAGCCC
    CCAGGACGAC CCACTGGAAG CCGGGGTAAC CAGCCTGCAG ATGATGGCTG GAATACAGTG
    CCCATCAGTA AGGGCAGCCG TCCTATTGAC ACCACTAGGA TCACAAAGAT AACAAAGCTT
    GGTGCTATTG ATTCAAATCA ACTGCTTGCC CCAGGAGGAC GTCTGAGTTG GGGTAAAGGA
    AGCAGCGGAG GAACTGGAGC CAAACCAGCA GAAACAGCTC CAGAATCTAG TCGGCCTGCG
    ACCAGCACCT TAAACAGGTT TTCTGCCTTG CAGCAGTCTT CATCTGCTGA GAGTCAAGAT
    GCTAGACGGC CCTTGCATCG GGGAAGTTCT AGTCGGGAAC GTTCTGAAAA AGATCGTGGA
    GACAGATTTG ATAGAGGTGA AAGGTTTGAT AAGGGAGAAC TGTTTGATAG ATCTGACCGT
    TCAGATCGAG CTCGTCCTCT TCTTACAAAG AGAAGCTATA GCAAGGAAAC TGAGGACCGT
    AGCCGAGAAA GAGAGGCCCA TATACCACAG GAGGCTGTGA GGAAGGTGGC AAGTATGACT
    GAGCAGCGGG ACCGCAGCAA AGAAAGTGTG AAAAAAGAAA TTCCAGCACC GACAGCAGTG
    GTTGCCTCAT CAAAGCCCTC TATGTCTGAG GATGAGCTAG AGAAGAAATC TAAAGCCATC
    ATAGAAGAAT ATTTGCACAT TAATGATATG AAGGAAGCTC TTCAGTGTGT AGTGGAGCTT
    AATAGTTCAA CGCTGCTTTT TATCTTTGTG CGGAATGGGA TTGAGTCAAC ACTAGAGAGA
    AGCACTATTG CCAGAGAGCA TATGGGTCTC CTTTTGTACC AGTTAGTAAA GGCAGGAACT
    CTGTCCAAGG AGCAGTATTA CAAAGGGGTG CTTGAAGTTC TTGAAGTAGC AGAAGATATG
    GAAATAGATA TACCCCACAT CTGGCTGTAT TTAGCAGAGA TAATCAGCCC TGTGCTGCTA
    GAGGGGGGGA TTCCGATGGG GGAGCTGTTC AGAGAGCTGA CCAAACCTTT AGTGCTCATT
    GGCAAAGCTG GAGTCCTTCT GGCAGAAATC CTTGGATTAT TGTGCAAAGG CATGAGTCAT
    AAGAAGGCTG GAGCCCTGTG GAAAGAATCT GGGCTAAGCT GGAAGGAATT CTTGTCCAAG
    GACCAGGATA TTAACAAGTT TATAACTGAC AAGAAACTCG AATACACAAC AGGAGAGGAG
    ACTGAAACAC CCAGTAAGAA AGACCTCACC CCTGCTGAAC TTGATAAAAT TTTGACAAAA
    CTTTTGAAAG AGAACAGCAG TAACCAGAAG ATATTTGACT GGATTGAAGC CAACTTAAAT
    GACCAGCAAA CTTCATCCAA CACCTTTGTT CGAACTTTAA TGAGTACTGT GTGCCACTCT
    GCTATTATTT TTGAGAATCC TTGTAGGGTT GATGTTAATA TTATCCATAC TAGAGCCAAA
    TTTCTTCAGA AATATTTAAG TGACGAGGCC AAGGAACTAC AGGCTCTCTA TGCTCTACAG
    GCACTTGTTG TAAAGCTTGA CCAGCCCCCA AATCTCCTAC GGATGTTTTT TGATGCTCTC
    TATGATGAGG ATGTGATAAA AGAGGATCCA TTCTACAAGT GGGAGTCCAG CAAAGACCCA
    GCTGAACAGC AAGGAAAAGG TGTTGCTCTT AAATCTGTCA CTGCCTTCTT CACATGGCTA
    CGGGAAGCAG AGGATGAGTC TGATAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012818831.1
    CDS136..4965
    Translation

    Target ORF information:

    RefSeq Version XM_012963377.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012963377.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
    CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA
    CAAATGAATA CACAATCTCA GCCTCGACAG GCAGGATTCA GATCAATCCA GCATTATTAT
    CAAAATAGGA CACAGCCTCC CACTAGTGCC ACCAGAGTGC AGAGTGGTTC TTCTGCTCGA
    GCAGTTACTC CAGCCCATGT GTATCAACCA GGCTCCCAAG TTATGATGAT TCCTTCACAG
    ATTTCATACC CTTCCCAGGG ATACTACATT CAGGGACAGG GACGCTCATA TGTGGTGCCC
    ACAAACCAGT ACCCTGTGCA GACTGGGGCT CCAGGTTTCT TCCCCAGTGC AAGTCCCTCA
    GAATATGGCA CATATGCTGG AGCATATTAC CCTGCACAAC AGACGTTCCC TCCTGGAGTG
    CCTGGAGCAC AAGTTATGAT GAATCAGCCA CAGGTGATCC CTCCTAAACG GGAGCGCAAA
    ACGATAAGAA TTAGAGACCC CAACCAAGGA GGCAAAGATA TCACAGAAGA AATTCTCTCA
    GGAGGAAGAA CATCCTCTAC GCCAACGCCT CCACAGTCAA CTGTGGGAAG TTCTGAAATT
    CAAGCTAATG GAGAAAGCGT TCATCATGTT ACCGTTGTTG TGCGCCCAGA TGATGGACCC
    AAGGCCACCC AAGTGCTGAT CAAAGCCTCA GAGAGCCCTT CCCCTCCTCT GCCTCTCTCT
    GAAGCTGAGC CAAGCATAGC AAATGTGTGT GATATTTCAA AAGATAACAC TATCTCTGAG
    TTGACTTCTC ATATAGTAGA GCCCCTAAGT TCAGAAGAGA CAACCATTGA TACTTCTACC
    TCTCAGGTGG ATCTCAAACA CCAGCCAGTG GCTGCTATGG CAGAGCATGA AACTCTTATA
    GAGGAGGCAT CAATACAGGA GGTGGCCTTT GCAGAGGCAC CCATCGCTCA ATCAGAGATA
    CCAAAATCTC TTGCCTCCCA GCCTCTAGCA GAAATCTCTA CCCCTGAACA GATTTCTAAA
    CTTGACGCAC AAGTGCCATC TTTGCAACAA GAGGAGTTAC TTACAGAAAC TGTTGAACTT
    AATTTAAGCA ATCAGGCTCT TCCTTCACAA ATTTCTGAGC CTGAACCTTT TGAACCTGAG
    GCTTCAGCTT CAGATCCTCT ACATGAGGAG CCCACAGAGC CCACAGTTTC AACAGAAGAA
    CCACAAGCTA ATGGACTGTC TGAGGAGACA CCAGTATTAC CTGTACAGCC ACAACCAACA
    TCTGTTTGCC AGACTGAGCC CTCCCTAGAA TCCCCCATCG CTCAGCCTGA GGAGCTGCTT
    CTAACAAATG GAGGTGGACC ACCTGGCCTA CAAGAAAATG ATGCACCTGA TTCACAGGTT
    GAAGCTCATG TTAGCCCAAT CACTGAGCCT GAGGACCAAC CTGTGACAGT CTCTGATAAC
    GTGCTTGTGG AAGAGGATGT AGAAAAAACA TTGCCTGCGG ATACGGAAGT CGTGGCTTCA
    ACCCAAGTTG TAAAGCAGAG CTCCGAGAGT CAGTCTCAGG TTGCTGTTGC TGCACCCAAG
    AAAAAGCGAA AAATGAAGGA ACTAAACAAG AAAGAGGCAG TGGGAGATCT CCTTGATGCG
    TTCAAAGAGG CTGGTGATGG CTCTTCTGAT GTGGATTCTT CCTCTAAGCC TACCAGTGCT
    GCACCTGAAC CAGATCAGAG CCCTTGTGCT CCTCCTGAGG AACAGGATGA AACTTGGGAA
    GAGAAGGAAG ATAAACTGGA TGCAGAGAAT ATTAAACCAG GAGAACATAA ATATAAATAC
    AAAGAAGAGC AATGGAAACC CCTAAACCCG GAGGAAAAGA AGAGTTATGA TCGCGAGTTT
    CTGCTAGGGT TCCAGTTTAT TGTGGCCAGT ATGCAGAAAC CTGCGGGTCT TCCTCAAATT
    ACTGATGTGG TGCTGGATAA GGCAAACAAA ACTCCATTGC GACCATTGGA TCCTACACGT
    CTGACGGGAA TGAACTGTGG GCCAGATTTT ACACCTTCAT TTGCCAACCT TGGTCGGCCT
    GCTGTGGGTA ACCGTGGACC ACCAGCTGGC ATGGGTCCGA GACGGTCCCA GCAGGGTCCA
    CGAAAAGAGC CTCGTAAAAT TATTCAAACC GTATCATTAA ATGAGAATGT ACAACTAAAC
    AAAGCTGAAA AGGCATGGAA ACCGATAAAC AAGAGAGCTG GCGATGAGGA GGACCCAGAA
    AATATTAAGA CACAGGACCT ATTCCGAAGG GTTCGCAGTA TCTTGAATAA GCTGACCCCT
    CAGATGTTCC AGCAGCTGAT GAAGCAGGTC ACTGACCTTA CTATTGATAC CGAAGATCGT
    CTTAAGGGTG TCATTGACCT AATTTTTGAA AAAGCCATTT CAGAACCCAA TTTCTCTGTT
    GCATATGCCA ACATGTGTCG TTGCCTTATG GGGCTTAAAG TTCCAACCAC AGACAAGCCA
    GGGGTTACAG TCAACTTCCG CAAGTTGTTA CTGAATCGGT GCCAGAAGGA GTTTGAGAAG
    GATAAGGATG ATGATGAAGT GTTTGAGAAG AAACAAAAAG AAATGGATGC TGCTGCTTCG
    CCGGAGGAAA AAGCACGTTT GAATGATGAA CTGGTCGAGG CCCGTGACAG TGCACGACGG
    CGATCCTTGG GGAACATTAA GTTCATTGGA GAACTGTTTA AGTTAAAAAT GCTGACTGAA
    GCTATAATGC ACGATTGTGT GGTGAAGCTA TTAAAAAATC ATGATGAGGA ATCTCTGGAG
    TGCCTCTGCC GACTGCTGTC CACCATTGGC AAGGACCTGG ATTTTGAGAG AGCTAAGCCA
    CGTATGGACC AGTACTTCAA CCAGATGGAT AAAATCATTA AGGAGCGGAA GACATCTTCA
    CGAATTAGAT TCATGCTGCA GGATGTGATT GATTTGCGTC TGTGTAATTG GGTGCCTCGT
    CGTGCAGATC AGGGCCCTAA AACTATAGAT CAGATTCATA AAGAAGCAGA AATGGAAAAG
    CAGAGGGAGC AGGTTAAGGT GCAACAACTG ATGTCTAAAC AAGACAAGCG GCGAGAGCCC
    CCAGGACGAC CCACTGGAAG CCGGGGTAAC CAGCCTGCAG ATGATGGCTG GAATACAGTG
    CCCATCAGTA AGGGCAGCCG TCCTATTGAC ACCACTAGGA TCACAAAGAT AACAAAGCTT
    GGTGCTATTG ATTCAAATCA ACTGCTTGCC CCAGGAGGAC GTCTGAGTTG GGGTAAAGGA
    AGCAGCGGAG GAACTGGAGC CAAACCAGCA GAAACAGCTC CAGAATCTAG TCGGCCTGCG
    ACCAGCACCT TAAACAGGTT TTCTGCCTTG CAGCAGTCTT CATCTGCTGA GAGTCAAGAT
    GCTAGACGGC CCTTGCATCG GGGAAGTTCT AGTCGGGAAC GTTCTGAAAA AGATCGTGGA
    GACAGATTTG ATAGAGGTGA AAGGTTTGAT AAGGGAGAAC TGTTTGATAG ATCTGACCGT
    TCAGATCGAG CTCGTCCTCT TCTTACAAAG AGAAGCTATA GCAAGGAAAC TGAGGACCGT
    AGCCGAGAAA GAGAGGCCCA TATACCACAG GAGGCTGTGA GGAAGGTGGC AAGTATGACT
    GAGCAGCGGG ACCGCAGCAA AGAAAGTGTG AAAAAAGAAA TTCCAGCACC GACAGCAGTG
    GTTGCCTCAT CAAAGCCCTC TATGTCTGAG GATGAGCTAG AGAAGAAATC TAAAGCCATC
    ATAGAAGAAT ATTTGCACAT TAATGATATG AAGGAAGCTC TTCAGTGTGT AGTGGAGCTT
    AATAGTTCAA CGCTGCTTTT TATCTTTGTG CGGAATGGGA TTGAGTCAAC ACTAGAGAGA
    AGCACTATTG CCAGAGAGCA TATGGGTCTC CTTTTGTACC AGTTAGTAAA GGCAGGAACT
    CTGTCCAAGG AGCAGTATTA CAAAGGGGTG CTTGAAGTTC TTGAAGTAGC AGAAGATATG
    GAAATAGATA TACCCCACAT CTGGCTGTAT TTAGCAGAGA TAATCAGCCC TGTGCTGCTA
    GAGGGGGGGA TTCCGATGGG GGAGCTGTTC AGAGAGCTGA CCAAACCTTT AGTGCTCATT
    GGCAAAGCTG GAGTCCTTCT GGCAGAAATC CTTGGATTAT TGTGCAAAGG CATGAGTCAT
    AAGAAGGCTG GAGCCCTGTG GAAAGAATCT GGGCTAAGCT GGAAGGAATT CTTGTCCAAG
    GACCAGGATA TTAACAAGTT TATAACTGAC AAGAAACTCG AATACACAAC AGGAGAGGAG
    ACTGAAACAC CCAGTAAGAA AGACCTCACC CCTGCTGAAC TTGATAAAAT TTTGACAAAA
    CTTTTGAAAG AGAACAGCAG TAACCAGAAG ATATTTGACT GGATTGAAGC CAACTTAAAT
    GACCAGCAAA CTTCATCCAA CACCTTTGTT CGAACTTTAA TGAGTACTGT GTGCCACTCT
    GCTATTATTT TTGAGAATCC TTGTAGGGTT GATGTTAATA TTATCCATAC TAGAGCCAAA
    TTTCTTCAGA AATATTTAAG TGACGAGGCC AAGGAACTAC AGGCTCTCTA TGCTCTACAG
    GCACTTGTTG TAAAGCTTGA CCAGCCCCCA AATCTCCTAC GGATGTTTTT TGATGCTCTC
    TATGATGAGG ATGTGATAAA AGAGGATCCA TTCTACAAGT GGGAGTCCAG CAAAGACCCA
    GCTGAACAGC AAGGAAAAGG TGTTGCTCTT AAATCTGTCA CTGCCTTCTT CACATGGCTA
    CGGGAAGCAG AGGATGAGTC TGATAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa10205
    Clone ID Related Accession (Same CDS sequence) XM_004914401.4 , XM_004914400.3 , XM_004914401.3 , XM_012963378.2 , XM_012963378.3 , XM_004914400.4
    Accession Version XM_004914400.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4809bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004914400.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
    CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA
    CAAATGAATA CACAATCTCA GCCTCGACAG CATTATTATC AAAATAGGAC ACAGCCTCCC
    ACTAGTGCCA CCAGAGTGCA GAGTGGTTCT TCTGCTCGAG CAGTTACTCC AGCCCATGTG
    TATCAACCAG GCTCCCAAGT TATGATGATT CCTTCACAGA TTTCATACCC TTCCCAGGGA
    TACTACATTC AGGGACAGGG ACGCTCATAT GTGGTGCCCA CAAACCAGTA CCCTGTGCAG
    ACTGGGGCTC CAGGTTTCTT CCCCAGTGCA AGTCCCTCAG AATATGGCAC ATATGCTGGA
    GCATATTACC CTGCACAACA GACGTTCCCT CCTGGAGTGC CTGGAGCACA AGTTATGATG
    AATCAGCCAC AGGTGATCCC TCCTAAACGG GAGCGCAAAA CGATAAGAAT TAGAGACCCC
    AACCAAGGAG GCAAAGATAT CACAGAAGAA ATTCTCTCAG GAGGAAGAAC ATCCTCTACG
    CCAACGCCTC CACAGTCAAC TGTGGGAAGT TCTGAAATTC AAGCTAATGG AGAAAGCGTT
    CATCATGTTA CCGTTGTTGT GCGCCCAGAT GATGGACCCA AGGCCACCCA AGTGCTGATC
    AAAGCCTCAG AGAGCCCTTC CCCTCCTCTG CCTCTCTCTG AAGCTGAGCC AAGCATAGCA
    AATGTGTGTG ATATTTCAAA AGATAACACT ATCTCTGAGT TGACTTCTCA TATAGTAGAG
    CCCCTAAGTT CAGAAGAGAC AACCATTGAT ACTTCTACCT CTCAGGTGGA TCTCAAACAC
    CAGCCAGTGG CTGCTATGGC AGAGCATGAA ACTCTTATAG AGGAGGCATC AATACAGGAG
    GTGGCCTTTG CAGAGGCACC CATCGCTCAA TCAGAGATAC CAAAATCTCT TGCCTCCCAG
    CCTCTAGCAG AAATCTCTAC CCCTGAACAG ATTTCTAAAC TTGACGCACA AGTGCCATCT
    TTGCAACAAG AGGAGTTACT TACAGAAACT GTTGAACTTA ATTTAAGCAA TCAGGCTCTT
    CCTTCACAAA TTTCTGAGCC TGAACCTTTT GAACCTGAGG CTTCAGCTTC AGATCCTCTA
    CATGAGGAGC CCACAGAGCC CACAGTTTCA ACAGAAGAAC CACAAGCTAA TGGACTGTCT
    GAGGAGACAC CAGTATTACC TGTACAGCCA CAACCAACAT CTGTTTGCCA GACTGAGCCC
    TCCCTAGAAT CCCCCATCGC TCAGCCTGAG GAGCTGCTTC TAACAAATGG AGGTGGACCA
    CCTGGCCTAC AAGAAAATGA TGCACCTGAT TCACAGGTTG AAGCTCATGT TAGCCCAATC
    ACTGAGCCTG AGGACCAACC TGTGACAGTC TCTGATAACG TGCTTGTGGA AGAGGATGTA
    GAAAAAACAT TGCCTGCGGA TACGGAAGTC GTGGCTTCAA CCCAAGTTGT AAAGCAGAGC
    TCCGAGAGTC AGTCTCAGGT TGCTGTTGCT GCACCCAAGA AAAAGCGAAA AATGAAGGAA
    CTAAACAAGA AAGAGGCAGT GGGAGATCTC CTTGATGCGT TCAAAGAGGC TGGTGATGGC
    TCTTCTGATG TGGATTCTTC CTCTAAGCCT ACCAGTGCTG CACCTGAACC AGATCAGAGC
    CCTTGTGCTC CTCCTGAGGA ACAGGATGAA ACTTGGGAAG AGAAGGAAGA TAAACTGGAT
    GCAGAGAATA TTAAACCAGG AGAACATAAA TATAAATACA AAGAAGAGCA ATGGAAACCC
    CTAAACCCGG AGGAAAAGAA GAGTTATGAT CGCGAGTTTC TGCTAGGGTT CCAGTTTATT
    GTGGCCAGTA TGCAGAAACC TGCGGGTCTT CCTCAAATTA CTGATGTGGT GCTGGATAAG
    GCAAACAAAA CTCCATTGCG ACCATTGGAT CCTACACGTC TGACGGGAAT GAACTGTGGG
    CCAGATTTTA CACCTTCATT TGCCAACCTT GGTCGGCCTG CTGTGGGTAA CCGTGGACCA
    CCAGCTGGCA TGGGTCCGAG ACGGTCCCAG CAGGGTCCAC GAAAAGAGCC TCGTAAAATT
    ATTCAAACCG TATCATTAAA TGAGAATGTA CAACTAAACA AAGCTGAAAA GGCATGGAAA
    CCGATAAACA AGAGAGCTGG CGATGAGGAG GACCCAGAAA ATATTAAGAC ACAGGACCTA
    TTCCGAAGGG TTCGCAGTAT CTTGAATAAG CTGACCCCTC AGATGTTCCA GCAGCTGATG
    AAGCAGGTCA CTGACCTTAC TATTGATACC GAAGATCGTC TTAAGGGTGT CATTGACCTA
    ATTTTTGAAA AAGCCATTTC AGAACCCAAT TTCTCTGTTG CATATGCCAA CATGTGTCGT
    TGCCTTATGG GGCTTAAAGT TCCAACCACA GACAAGCCAG GGGTTACAGT CAACTTCCGC
    AAGTTGTTAC TGAATCGGTG CCAGAAGGAG TTTGAGAAGG ATAAGGATGA TGATGAAGTG
    TTTGAGAAGA AACAAAAAGA AATGGATGCT GCTGCTTCGC CGGAGGAAAA AGCACGTTTG
    AATGATGAAC TGGTCGAGGC CCGTGACAGT GCACGACGGC GATCCTTGGG GAACATTAAG
    TTCATTGGAG AACTGTTTAA GTTAAAAATG CTGACTGAAG CTATAATGCA CGATTGTGTG
    GTGAAGCTAT TAAAAAATCA TGATGAGGAA TCTCTGGAGT GCCTCTGCCG ACTGCTGTCC
    ACCATTGGCA AGGACCTGGA TTTTGAGAGA GCTAAGCCAC GTATGGACCA GTACTTCAAC
    CAGATGGATA AAATCATTAA GGAGCGGAAG ACATCTTCAC GAATTAGATT CATGCTGCAG
    GATGTGATTG ATTTGCGTCT GTGTAATTGG GTGCCTCGTC GTGCAGATCA GGGCCCTAAA
    ACTATAGATC AGATTCATAA AGAAGCAGAA ATGGAAAAGC AGAGGGAGCA GGTTAAGGTG
    CAACAACTGA TGTCTAAACA AGACAAGCGG CGAGAGCCCC CAGGACGACC CACTGGAAGC
    CGGGGTAACC AGCCTGCAGA TGATGGCTGG AATACAGTGC CCATCAGTAA GGGCAGCCGT
    CCTATTGACA CCACTAGGAT CACAAAGATA ACAAAGCTTG GTGCTATTGA TTCAAATCAA
    CTGCTTGCCC CAGGAGGACG TCTGAGTTGG GGTAAAGGAA GCAGCGGAGG AACTGGAGCC
    AAACCAGCAG AAACAGCTCC AGAATCTAGT CGGCCTGCGA CCAGCACCTT AAACAGGTTT
    TCTGCCTTGC AGCAGTCTTC ATCTGCTGAG AGTCAAGATG CTAGACGGCC CTTGCATCGG
    GGAAGTTCTA GTCGGGAACG TTCTGAAAAA GATCGTGGAG ACAGATTTGA TAGAGGTGAA
    AGGTTTGATA AGGGAGAACT GTTTGATAGA TCTGACCGTT CAGATCGAGC TCGTCCTCTT
    CTTACAAAGA GAAGCTATAG CAAGGAAACT GAGGACCGTA GCCGAGAAAG AGAGGCCCAT
    ATACCACAGG AGGCTGTGAG GAAGGTGGCA AGTATGACTG AGCAGCGGGA CCGCAGCAAA
    GAAAGTGTGA AAAAAGAAAT TCCAGCACCG ACAGCAGTGG TTGCCTCATC AAAGCCCTCT
    ATGTCTGAGG ATGAGCTAGA GAAGAAATCT AAAGCCATCA TAGAAGAATA TTTGCACATT
    AATGATATGA AGGAAGCTCT TCAGTGTGTA GTGGAGCTTA ATAGTTCAAC GCTGCTTTTT
    ATCTTTGTGC GGAATGGGAT TGAGTCAACA CTAGAGAGAA GCACTATTGC CAGAGAGCAT
    ATGGGTCTCC TTTTGTACCA GTTAGTAAAG GCAGGAACTC TGTCCAAGGA GCAGTATTAC
    AAAGGGGTGC TTGAAGTTCT TGAAGTAGCA GAAGATATGG AAATAGATAT ACCCCACATC
    TGGCTGTATT TAGCAGAGAT AATCAGCCCT GTGCTGCTAG AGGGGGGGAT TCCGATGGGG
    GAGCTGTTCA GAGAGCTGAC CAAACCTTTA GTGCTCATTG GCAAAGCTGG AGTCCTTCTG
    GCAGAAATCC TTGGATTATT GTGCAAAGGC ATGAGTCATA AGAAGGCTGG AGCCCTGTGG
    AAAGAATCTG GGCTAAGCTG GAAGGAATTC TTGTCCAAGG ACCAGGATAT TAACAAGTTT
    ATAACTGACA AGAAACTCGA ATACACAACA GGAGAGGAGA CTGAAACACC CAGTAAGAAA
    GACCTCACCC CTGCTGAACT TGATAAAATT TTGACAAAAC TTTTGAAAGA GAACAGCAGT
    AACCAGAAGA TATTTGACTG GATTGAAGCC AACTTAAATG ACCAGCAAAC TTCATCCAAC
    ACCTTTGTTC GAACTTTAAT GAGTACTGTG TGCCACTCTG CTATTATTTT TGAGAATCCT
    TGTAGGGTTG ATGTTAATAT TATCCATACT AGAGCCAAAT TTCTTCAGAA ATATTTAAGT
    GACGAGGCCA AGGAACTACA GGCTCTCTAT GCTCTACAGG CACTTGTTGT AAAGCTTGAC
    CAGCCCCCAA ATCTCCTACG GATGTTTTTT GATGCTCTCT ATGATGAGGA TGTGATAAAA
    GAGGATCCAT TCTACAAGTG GGAGTCCAGC AAAGACCCAG CTGAACAGCA AGGAAAAGGT
    GTTGCTCTTA AATCTGTCAC TGCCTTCTTC ACATGGCTAC GGGAAGCAGA GGATGAGTCT
    GATAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004914457.1
    CDS215..5023
    Translation

    Target ORF information:

    RefSeq Version XM_004914400.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004914400.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
    CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA
    CAAATGAATA CACAATCTCA GCCTCGACAG CATTATTATC AAAATAGGAC ACAGCCTCCC
    ACTAGTGCCA CCAGAGTGCA GAGTGGTTCT TCTGCTCGAG CAGTTACTCC AGCCCATGTG
    TATCAACCAG GCTCCCAAGT TATGATGATT CCTTCACAGA TTTCATACCC TTCCCAGGGA
    TACTACATTC AGGGACAGGG ACGCTCATAT GTGGTGCCCA CAAACCAGTA CCCTGTGCAG
    ACTGGGGCTC CAGGTTTCTT CCCCAGTGCA AGTCCCTCAG AATATGGCAC ATATGCTGGA
    GCATATTACC CTGCACAACA GACGTTCCCT CCTGGAGTGC CTGGAGCACA AGTTATGATG
    AATCAGCCAC AGGTGATCCC TCCTAAACGG GAGCGCAAAA CGATAAGAAT TAGAGACCCC
    AACCAAGGAG GCAAAGATAT CACAGAAGAA ATTCTCTCAG GAGGAAGAAC ATCCTCTACG
    CCAACGCCTC CACAGTCAAC TGTGGGAAGT TCTGAAATTC AAGCTAATGG AGAAAGCGTT
    CATCATGTTA CCGTTGTTGT GCGCCCAGAT GATGGACCCA AGGCCACCCA AGTGCTGATC
    AAAGCCTCAG AGAGCCCTTC CCCTCCTCTG CCTCTCTCTG AAGCTGAGCC AAGCATAGCA
    AATGTGTGTG ATATTTCAAA AGATAACACT ATCTCTGAGT TGACTTCTCA TATAGTAGAG
    CCCCTAAGTT CAGAAGAGAC AACCATTGAT ACTTCTACCT CTCAGGTGGA TCTCAAACAC
    CAGCCAGTGG CTGCTATGGC AGAGCATGAA ACTCTTATAG AGGAGGCATC AATACAGGAG
    GTGGCCTTTG CAGAGGCACC CATCGCTCAA TCAGAGATAC CAAAATCTCT TGCCTCCCAG
    CCTCTAGCAG AAATCTCTAC CCCTGAACAG ATTTCTAAAC TTGACGCACA AGTGCCATCT
    TTGCAACAAG AGGAGTTACT TACAGAAACT GTTGAACTTA ATTTAAGCAA TCAGGCTCTT
    CCTTCACAAA TTTCTGAGCC TGAACCTTTT GAACCTGAGG CTTCAGCTTC AGATCCTCTA
    CATGAGGAGC CCACAGAGCC CACAGTTTCA ACAGAAGAAC CACAAGCTAA TGGACTGTCT
    GAGGAGACAC CAGTATTACC TGTACAGCCA CAACCAACAT CTGTTTGCCA GACTGAGCCC
    TCCCTAGAAT CCCCCATCGC TCAGCCTGAG GAGCTGCTTC TAACAAATGG AGGTGGACCA
    CCTGGCCTAC AAGAAAATGA TGCACCTGAT TCACAGGTTG AAGCTCATGT TAGCCCAATC
    ACTGAGCCTG AGGACCAACC TGTGACAGTC TCTGATAACG TGCTTGTGGA AGAGGATGTA
    GAAAAAACAT TGCCTGCGGA TACGGAAGTC GTGGCTTCAA CCCAAGTTGT AAAGCAGAGC
    TCCGAGAGTC AGTCTCAGGT TGCTGTTGCT GCACCCAAGA AAAAGCGAAA AATGAAGGAA
    CTAAACAAGA AAGAGGCAGT GGGAGATCTC CTTGATGCGT TCAAAGAGGC TGGTGATGGC
    TCTTCTGATG TGGATTCTTC CTCTAAGCCT ACCAGTGCTG CACCTGAACC AGATCAGAGC
    CCTTGTGCTC CTCCTGAGGA ACAGGATGAA ACTTGGGAAG AGAAGGAAGA TAAACTGGAT
    GCAGAGAATA TTAAACCAGG AGAACATAAA TATAAATACA AAGAAGAGCA ATGGAAACCC
    CTAAACCCGG AGGAAAAGAA GAGTTATGAT CGCGAGTTTC TGCTAGGGTT CCAGTTTATT
    GTGGCCAGTA TGCAGAAACC TGCGGGTCTT CCTCAAATTA CTGATGTGGT GCTGGATAAG
    GCAAACAAAA CTCCATTGCG ACCATTGGAT CCTACACGTC TGACGGGAAT GAACTGTGGG
    CCAGATTTTA CACCTTCATT TGCCAACCTT GGTCGGCCTG CTGTGGGTAA CCGTGGACCA
    CCAGCTGGCA TGGGTCCGAG ACGGTCCCAG CAGGGTCCAC GAAAAGAGCC TCGTAAAATT
    ATTCAAACCG TATCATTAAA TGAGAATGTA CAACTAAACA AAGCTGAAAA GGCATGGAAA
    CCGATAAACA AGAGAGCTGG CGATGAGGAG GACCCAGAAA ATATTAAGAC ACAGGACCTA
    TTCCGAAGGG TTCGCAGTAT CTTGAATAAG CTGACCCCTC AGATGTTCCA GCAGCTGATG
    AAGCAGGTCA CTGACCTTAC TATTGATACC GAAGATCGTC TTAAGGGTGT CATTGACCTA
    ATTTTTGAAA AAGCCATTTC AGAACCCAAT TTCTCTGTTG CATATGCCAA CATGTGTCGT
    TGCCTTATGG GGCTTAAAGT TCCAACCACA GACAAGCCAG GGGTTACAGT CAACTTCCGC
    AAGTTGTTAC TGAATCGGTG CCAGAAGGAG TTTGAGAAGG ATAAGGATGA TGATGAAGTG
    TTTGAGAAGA AACAAAAAGA AATGGATGCT GCTGCTTCGC CGGAGGAAAA AGCACGTTTG
    AATGATGAAC TGGTCGAGGC CCGTGACAGT GCACGACGGC GATCCTTGGG GAACATTAAG
    TTCATTGGAG AACTGTTTAA GTTAAAAATG CTGACTGAAG CTATAATGCA CGATTGTGTG
    GTGAAGCTAT TAAAAAATCA TGATGAGGAA TCTCTGGAGT GCCTCTGCCG ACTGCTGTCC
    ACCATTGGCA AGGACCTGGA TTTTGAGAGA GCTAAGCCAC GTATGGACCA GTACTTCAAC
    CAGATGGATA AAATCATTAA GGAGCGGAAG ACATCTTCAC GAATTAGATT CATGCTGCAG
    GATGTGATTG ATTTGCGTCT GTGTAATTGG GTGCCTCGTC GTGCAGATCA GGGCCCTAAA
    ACTATAGATC AGATTCATAA AGAAGCAGAA ATGGAAAAGC AGAGGGAGCA GGTTAAGGTG
    CAACAACTGA TGTCTAAACA AGACAAGCGG CGAGAGCCCC CAGGACGACC CACTGGAAGC
    CGGGGTAACC AGCCTGCAGA TGATGGCTGG AATACAGTGC CCATCAGTAA GGGCAGCCGT
    CCTATTGACA CCACTAGGAT CACAAAGATA ACAAAGCTTG GTGCTATTGA TTCAAATCAA
    CTGCTTGCCC CAGGAGGACG TCTGAGTTGG GGTAAAGGAA GCAGCGGAGG AACTGGAGCC
    AAACCAGCAG AAACAGCTCC AGAATCTAGT CGGCCTGCGA CCAGCACCTT AAACAGGTTT
    TCTGCCTTGC AGCAGTCTTC ATCTGCTGAG AGTCAAGATG CTAGACGGCC CTTGCATCGG
    GGAAGTTCTA GTCGGGAACG TTCTGAAAAA GATCGTGGAG ACAGATTTGA TAGAGGTGAA
    AGGTTTGATA AGGGAGAACT GTTTGATAGA TCTGACCGTT CAGATCGAGC TCGTCCTCTT
    CTTACAAAGA GAAGCTATAG CAAGGAAACT GAGGACCGTA GCCGAGAAAG AGAGGCCCAT
    ATACCACAGG AGGCTGTGAG GAAGGTGGCA AGTATGACTG AGCAGCGGGA CCGCAGCAAA
    GAAAGTGTGA AAAAAGAAAT TCCAGCACCG ACAGCAGTGG TTGCCTCATC AAAGCCCTCT
    ATGTCTGAGG ATGAGCTAGA GAAGAAATCT AAAGCCATCA TAGAAGAATA TTTGCACATT
    AATGATATGA AGGAAGCTCT TCAGTGTGTA GTGGAGCTTA ATAGTTCAAC GCTGCTTTTT
    ATCTTTGTGC GGAATGGGAT TGAGTCAACA CTAGAGAGAA GCACTATTGC CAGAGAGCAT
    ATGGGTCTCC TTTTGTACCA GTTAGTAAAG GCAGGAACTC TGTCCAAGGA GCAGTATTAC
    AAAGGGGTGC TTGAAGTTCT TGAAGTAGCA GAAGATATGG AAATAGATAT ACCCCACATC
    TGGCTGTATT TAGCAGAGAT AATCAGCCCT GTGCTGCTAG AGGGGGGGAT TCCGATGGGG
    GAGCTGTTCA GAGAGCTGAC CAAACCTTTA GTGCTCATTG GCAAAGCTGG AGTCCTTCTG
    GCAGAAATCC TTGGATTATT GTGCAAAGGC ATGAGTCATA AGAAGGCTGG AGCCCTGTGG
    AAAGAATCTG GGCTAAGCTG GAAGGAATTC TTGTCCAAGG ACCAGGATAT TAACAAGTTT
    ATAACTGACA AGAAACTCGA ATACACAACA GGAGAGGAGA CTGAAACACC CAGTAAGAAA
    GACCTCACCC CTGCTGAACT TGATAAAATT TTGACAAAAC TTTTGAAAGA GAACAGCAGT
    AACCAGAAGA TATTTGACTG GATTGAAGCC AACTTAAATG ACCAGCAAAC TTCATCCAAC
    ACCTTTGTTC GAACTTTAAT GAGTACTGTG TGCCACTCTG CTATTATTTT TGAGAATCCT
    TGTAGGGTTG ATGTTAATAT TATCCATACT AGAGCCAAAT TTCTTCAGAA ATATTTAAGT
    GACGAGGCCA AGGAACTACA GGCTCTCTAT GCTCTACAGG CACTTGTTGT AAAGCTTGAC
    CAGCCCCCAA ATCTCCTACG GATGTTTTTT GATGCTCTCT ATGATGAGGA TGTGATAAAA
    GAGGATCCAT TCTACAAGTG GGAGTCCAGC AAAGACCCAG CTGAACAGCA AGGAAAAGGT
    GTTGCTCTTA AATCTGTCAC TGCCTTCTTC ACATGGCTAC GGGAAGCAGA GGATGAGTCT
    GATAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa10205
    Clone ID Related Accession (Same CDS sequence) XM_004914401.4 , XM_004914400.3 , XM_004914401.3 , XM_012963378.2 , XM_012963378.3 , XM_004914400.4
    Accession Version XM_004914401.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4809bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004914401.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
    CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA
    CAAATGAATA CACAATCTCA GCCTCGACAG CATTATTATC AAAATAGGAC ACAGCCTCCC
    ACTAGTGCCA CCAGAGTGCA GAGTGGTTCT TCTGCTCGAG CAGTTACTCC AGCCCATGTG
    TATCAACCAG GCTCCCAAGT TATGATGATT CCTTCACAGA TTTCATACCC TTCCCAGGGA
    TACTACATTC AGGGACAGGG ACGCTCATAT GTGGTGCCCA CAAACCAGTA CCCTGTGCAG
    ACTGGGGCTC CAGGTTTCTT CCCCAGTGCA AGTCCCTCAG AATATGGCAC ATATGCTGGA
    GCATATTACC CTGCACAACA GACGTTCCCT CCTGGAGTGC CTGGAGCACA AGTTATGATG
    AATCAGCCAC AGGTGATCCC TCCTAAACGG GAGCGCAAAA CGATAAGAAT TAGAGACCCC
    AACCAAGGAG GCAAAGATAT CACAGAAGAA ATTCTCTCAG GAGGAAGAAC ATCCTCTACG
    CCAACGCCTC CACAGTCAAC TGTGGGAAGT TCTGAAATTC AAGCTAATGG AGAAAGCGTT
    CATCATGTTA CCGTTGTTGT GCGCCCAGAT GATGGACCCA AGGCCACCCA AGTGCTGATC
    AAAGCCTCAG AGAGCCCTTC CCCTCCTCTG CCTCTCTCTG AAGCTGAGCC AAGCATAGCA
    AATGTGTGTG ATATTTCAAA AGATAACACT ATCTCTGAGT TGACTTCTCA TATAGTAGAG
    CCCCTAAGTT CAGAAGAGAC AACCATTGAT ACTTCTACCT CTCAGGTGGA TCTCAAACAC
    CAGCCAGTGG CTGCTATGGC AGAGCATGAA ACTCTTATAG AGGAGGCATC AATACAGGAG
    GTGGCCTTTG CAGAGGCACC CATCGCTCAA TCAGAGATAC CAAAATCTCT TGCCTCCCAG
    CCTCTAGCAG AAATCTCTAC CCCTGAACAG ATTTCTAAAC TTGACGCACA AGTGCCATCT
    TTGCAACAAG AGGAGTTACT TACAGAAACT GTTGAACTTA ATTTAAGCAA TCAGGCTCTT
    CCTTCACAAA TTTCTGAGCC TGAACCTTTT GAACCTGAGG CTTCAGCTTC AGATCCTCTA
    CATGAGGAGC CCACAGAGCC CACAGTTTCA ACAGAAGAAC CACAAGCTAA TGGACTGTCT
    GAGGAGACAC CAGTATTACC TGTACAGCCA CAACCAACAT CTGTTTGCCA GACTGAGCCC
    TCCCTAGAAT CCCCCATCGC TCAGCCTGAG GAGCTGCTTC TAACAAATGG AGGTGGACCA
    CCTGGCCTAC AAGAAAATGA TGCACCTGAT TCACAGGTTG AAGCTCATGT TAGCCCAATC
    ACTGAGCCTG AGGACCAACC TGTGACAGTC TCTGATAACG TGCTTGTGGA AGAGGATGTA
    GAAAAAACAT TGCCTGCGGA TACGGAAGTC GTGGCTTCAA CCCAAGTTGT AAAGCAGAGC
    TCCGAGAGTC AGTCTCAGGT TGCTGTTGCT GCACCCAAGA AAAAGCGAAA AATGAAGGAA
    CTAAACAAGA AAGAGGCAGT GGGAGATCTC CTTGATGCGT TCAAAGAGGC TGGTGATGGC
    TCTTCTGATG TGGATTCTTC CTCTAAGCCT ACCAGTGCTG CACCTGAACC AGATCAGAGC
    CCTTGTGCTC CTCCTGAGGA ACAGGATGAA ACTTGGGAAG AGAAGGAAGA TAAACTGGAT
    GCAGAGAATA TTAAACCAGG AGAACATAAA TATAAATACA AAGAAGAGCA ATGGAAACCC
    CTAAACCCGG AGGAAAAGAA GAGTTATGAT CGCGAGTTTC TGCTAGGGTT CCAGTTTATT
    GTGGCCAGTA TGCAGAAACC TGCGGGTCTT CCTCAAATTA CTGATGTGGT GCTGGATAAG
    GCAAACAAAA CTCCATTGCG ACCATTGGAT CCTACACGTC TGACGGGAAT GAACTGTGGG
    CCAGATTTTA CACCTTCATT TGCCAACCTT GGTCGGCCTG CTGTGGGTAA CCGTGGACCA
    CCAGCTGGCA TGGGTCCGAG ACGGTCCCAG CAGGGTCCAC GAAAAGAGCC TCGTAAAATT
    ATTCAAACCG TATCATTAAA TGAGAATGTA CAACTAAACA AAGCTGAAAA GGCATGGAAA
    CCGATAAACA AGAGAGCTGG CGATGAGGAG GACCCAGAAA ATATTAAGAC ACAGGACCTA
    TTCCGAAGGG TTCGCAGTAT CTTGAATAAG CTGACCCCTC AGATGTTCCA GCAGCTGATG
    AAGCAGGTCA CTGACCTTAC TATTGATACC GAAGATCGTC TTAAGGGTGT CATTGACCTA
    ATTTTTGAAA AAGCCATTTC AGAACCCAAT TTCTCTGTTG CATATGCCAA CATGTGTCGT
    TGCCTTATGG GGCTTAAAGT TCCAACCACA GACAAGCCAG GGGTTACAGT CAACTTCCGC
    AAGTTGTTAC TGAATCGGTG CCAGAAGGAG TTTGAGAAGG ATAAGGATGA TGATGAAGTG
    TTTGAGAAGA AACAAAAAGA AATGGATGCT GCTGCTTCGC CGGAGGAAAA AGCACGTTTG
    AATGATGAAC TGGTCGAGGC CCGTGACAGT GCACGACGGC GATCCTTGGG GAACATTAAG
    TTCATTGGAG AACTGTTTAA GTTAAAAATG CTGACTGAAG CTATAATGCA CGATTGTGTG
    GTGAAGCTAT TAAAAAATCA TGATGAGGAA TCTCTGGAGT GCCTCTGCCG ACTGCTGTCC
    ACCATTGGCA AGGACCTGGA TTTTGAGAGA GCTAAGCCAC GTATGGACCA GTACTTCAAC
    CAGATGGATA AAATCATTAA GGAGCGGAAG ACATCTTCAC GAATTAGATT CATGCTGCAG
    GATGTGATTG ATTTGCGTCT GTGTAATTGG GTGCCTCGTC GTGCAGATCA GGGCCCTAAA
    ACTATAGATC AGATTCATAA AGAAGCAGAA ATGGAAAAGC AGAGGGAGCA GGTTAAGGTG
    CAACAACTGA TGTCTAAACA AGACAAGCGG CGAGAGCCCC CAGGACGACC CACTGGAAGC
    CGGGGTAACC AGCCTGCAGA TGATGGCTGG AATACAGTGC CCATCAGTAA GGGCAGCCGT
    CCTATTGACA CCACTAGGAT CACAAAGATA ACAAAGCTTG GTGCTATTGA TTCAAATCAA
    CTGCTTGCCC CAGGAGGACG TCTGAGTTGG GGTAAAGGAA GCAGCGGAGG AACTGGAGCC
    AAACCAGCAG AAACAGCTCC AGAATCTAGT CGGCCTGCGA CCAGCACCTT AAACAGGTTT
    TCTGCCTTGC AGCAGTCTTC ATCTGCTGAG AGTCAAGATG CTAGACGGCC CTTGCATCGG
    GGAAGTTCTA GTCGGGAACG TTCTGAAAAA GATCGTGGAG ACAGATTTGA TAGAGGTGAA
    AGGTTTGATA AGGGAGAACT GTTTGATAGA TCTGACCGTT CAGATCGAGC TCGTCCTCTT
    CTTACAAAGA GAAGCTATAG CAAGGAAACT GAGGACCGTA GCCGAGAAAG AGAGGCCCAT
    ATACCACAGG AGGCTGTGAG GAAGGTGGCA AGTATGACTG AGCAGCGGGA CCGCAGCAAA
    GAAAGTGTGA AAAAAGAAAT TCCAGCACCG ACAGCAGTGG TTGCCTCATC AAAGCCCTCT
    ATGTCTGAGG ATGAGCTAGA GAAGAAATCT AAAGCCATCA TAGAAGAATA TTTGCACATT
    AATGATATGA AGGAAGCTCT TCAGTGTGTA GTGGAGCTTA ATAGTTCAAC GCTGCTTTTT
    ATCTTTGTGC GGAATGGGAT TGAGTCAACA CTAGAGAGAA GCACTATTGC CAGAGAGCAT
    ATGGGTCTCC TTTTGTACCA GTTAGTAAAG GCAGGAACTC TGTCCAAGGA GCAGTATTAC
    AAAGGGGTGC TTGAAGTTCT TGAAGTAGCA GAAGATATGG AAATAGATAT ACCCCACATC
    TGGCTGTATT TAGCAGAGAT AATCAGCCCT GTGCTGCTAG AGGGGGGGAT TCCGATGGGG
    GAGCTGTTCA GAGAGCTGAC CAAACCTTTA GTGCTCATTG GCAAAGCTGG AGTCCTTCTG
    GCAGAAATCC TTGGATTATT GTGCAAAGGC ATGAGTCATA AGAAGGCTGG AGCCCTGTGG
    AAAGAATCTG GGCTAAGCTG GAAGGAATTC TTGTCCAAGG ACCAGGATAT TAACAAGTTT
    ATAACTGACA AGAAACTCGA ATACACAACA GGAGAGGAGA CTGAAACACC CAGTAAGAAA
    GACCTCACCC CTGCTGAACT TGATAAAATT TTGACAAAAC TTTTGAAAGA GAACAGCAGT
    AACCAGAAGA TATTTGACTG GATTGAAGCC AACTTAAATG ACCAGCAAAC TTCATCCAAC
    ACCTTTGTTC GAACTTTAAT GAGTACTGTG TGCCACTCTG CTATTATTTT TGAGAATCCT
    TGTAGGGTTG ATGTTAATAT TATCCATACT AGAGCCAAAT TTCTTCAGAA ATATTTAAGT
    GACGAGGCCA AGGAACTACA GGCTCTCTAT GCTCTACAGG CACTTGTTGT AAAGCTTGAC
    CAGCCCCCAA ATCTCCTACG GATGTTTTTT GATGCTCTCT ATGATGAGGA TGTGATAAAA
    GAGGATCCAT TCTACAAGTG GGAGTCCAGC AAAGACCCAG CTGAACAGCA AGGAAAAGGT
    GTTGCTCTTA AATCTGTCAC TGCCTTCTTC ACATGGCTAC GGGAAGCAGA GGATGAGTCT
    GATAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004914458.1
    CDS195..5003
    Translation

    Target ORF information:

    RefSeq Version XM_004914401.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004914401.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
    CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA
    CAAATGAATA CACAATCTCA GCCTCGACAG CATTATTATC AAAATAGGAC ACAGCCTCCC
    ACTAGTGCCA CCAGAGTGCA GAGTGGTTCT TCTGCTCGAG CAGTTACTCC AGCCCATGTG
    TATCAACCAG GCTCCCAAGT TATGATGATT CCTTCACAGA TTTCATACCC TTCCCAGGGA
    TACTACATTC AGGGACAGGG ACGCTCATAT GTGGTGCCCA CAAACCAGTA CCCTGTGCAG
    ACTGGGGCTC CAGGTTTCTT CCCCAGTGCA AGTCCCTCAG AATATGGCAC ATATGCTGGA
    GCATATTACC CTGCACAACA GACGTTCCCT CCTGGAGTGC CTGGAGCACA AGTTATGATG
    AATCAGCCAC AGGTGATCCC TCCTAAACGG GAGCGCAAAA CGATAAGAAT TAGAGACCCC
    AACCAAGGAG GCAAAGATAT CACAGAAGAA ATTCTCTCAG GAGGAAGAAC ATCCTCTACG
    CCAACGCCTC CACAGTCAAC TGTGGGAAGT TCTGAAATTC AAGCTAATGG AGAAAGCGTT
    CATCATGTTA CCGTTGTTGT GCGCCCAGAT GATGGACCCA AGGCCACCCA AGTGCTGATC
    AAAGCCTCAG AGAGCCCTTC CCCTCCTCTG CCTCTCTCTG AAGCTGAGCC AAGCATAGCA
    AATGTGTGTG ATATTTCAAA AGATAACACT ATCTCTGAGT TGACTTCTCA TATAGTAGAG
    CCCCTAAGTT CAGAAGAGAC AACCATTGAT ACTTCTACCT CTCAGGTGGA TCTCAAACAC
    CAGCCAGTGG CTGCTATGGC AGAGCATGAA ACTCTTATAG AGGAGGCATC AATACAGGAG
    GTGGCCTTTG CAGAGGCACC CATCGCTCAA TCAGAGATAC CAAAATCTCT TGCCTCCCAG
    CCTCTAGCAG AAATCTCTAC CCCTGAACAG ATTTCTAAAC TTGACGCACA AGTGCCATCT
    TTGCAACAAG AGGAGTTACT TACAGAAACT GTTGAACTTA ATTTAAGCAA TCAGGCTCTT
    CCTTCACAAA TTTCTGAGCC TGAACCTTTT GAACCTGAGG CTTCAGCTTC AGATCCTCTA
    CATGAGGAGC CCACAGAGCC CACAGTTTCA ACAGAAGAAC CACAAGCTAA TGGACTGTCT
    GAGGAGACAC CAGTATTACC TGTACAGCCA CAACCAACAT CTGTTTGCCA GACTGAGCCC
    TCCCTAGAAT CCCCCATCGC TCAGCCTGAG GAGCTGCTTC TAACAAATGG AGGTGGACCA
    CCTGGCCTAC AAGAAAATGA TGCACCTGAT TCACAGGTTG AAGCTCATGT TAGCCCAATC
    ACTGAGCCTG AGGACCAACC TGTGACAGTC TCTGATAACG TGCTTGTGGA AGAGGATGTA
    GAAAAAACAT TGCCTGCGGA TACGGAAGTC GTGGCTTCAA CCCAAGTTGT AAAGCAGAGC
    TCCGAGAGTC AGTCTCAGGT TGCTGTTGCT GCACCCAAGA AAAAGCGAAA AATGAAGGAA
    CTAAACAAGA AAGAGGCAGT GGGAGATCTC CTTGATGCGT TCAAAGAGGC TGGTGATGGC
    TCTTCTGATG TGGATTCTTC CTCTAAGCCT ACCAGTGCTG CACCTGAACC AGATCAGAGC
    CCTTGTGCTC CTCCTGAGGA ACAGGATGAA ACTTGGGAAG AGAAGGAAGA TAAACTGGAT
    GCAGAGAATA TTAAACCAGG AGAACATAAA TATAAATACA AAGAAGAGCA ATGGAAACCC
    CTAAACCCGG AGGAAAAGAA GAGTTATGAT CGCGAGTTTC TGCTAGGGTT CCAGTTTATT
    GTGGCCAGTA TGCAGAAACC TGCGGGTCTT CCTCAAATTA CTGATGTGGT GCTGGATAAG
    GCAAACAAAA CTCCATTGCG ACCATTGGAT CCTACACGTC TGACGGGAAT GAACTGTGGG
    CCAGATTTTA CACCTTCATT TGCCAACCTT GGTCGGCCTG CTGTGGGTAA CCGTGGACCA
    CCAGCTGGCA TGGGTCCGAG ACGGTCCCAG CAGGGTCCAC GAAAAGAGCC TCGTAAAATT
    ATTCAAACCG TATCATTAAA TGAGAATGTA CAACTAAACA AAGCTGAAAA GGCATGGAAA
    CCGATAAACA AGAGAGCTGG CGATGAGGAG GACCCAGAAA ATATTAAGAC ACAGGACCTA
    TTCCGAAGGG TTCGCAGTAT CTTGAATAAG CTGACCCCTC AGATGTTCCA GCAGCTGATG
    AAGCAGGTCA CTGACCTTAC TATTGATACC GAAGATCGTC TTAAGGGTGT CATTGACCTA
    ATTTTTGAAA AAGCCATTTC AGAACCCAAT TTCTCTGTTG CATATGCCAA CATGTGTCGT
    TGCCTTATGG GGCTTAAAGT TCCAACCACA GACAAGCCAG GGGTTACAGT CAACTTCCGC
    AAGTTGTTAC TGAATCGGTG CCAGAAGGAG TTTGAGAAGG ATAAGGATGA TGATGAAGTG
    TTTGAGAAGA AACAAAAAGA AATGGATGCT GCTGCTTCGC CGGAGGAAAA AGCACGTTTG
    AATGATGAAC TGGTCGAGGC CCGTGACAGT GCACGACGGC GATCCTTGGG GAACATTAAG
    TTCATTGGAG AACTGTTTAA GTTAAAAATG CTGACTGAAG CTATAATGCA CGATTGTGTG
    GTGAAGCTAT TAAAAAATCA TGATGAGGAA TCTCTGGAGT GCCTCTGCCG ACTGCTGTCC
    ACCATTGGCA AGGACCTGGA TTTTGAGAGA GCTAAGCCAC GTATGGACCA GTACTTCAAC
    CAGATGGATA AAATCATTAA GGAGCGGAAG ACATCTTCAC GAATTAGATT CATGCTGCAG
    GATGTGATTG ATTTGCGTCT GTGTAATTGG GTGCCTCGTC GTGCAGATCA GGGCCCTAAA
    ACTATAGATC AGATTCATAA AGAAGCAGAA ATGGAAAAGC AGAGGGAGCA GGTTAAGGTG
    CAACAACTGA TGTCTAAACA AGACAAGCGG CGAGAGCCCC CAGGACGACC CACTGGAAGC
    CGGGGTAACC AGCCTGCAGA TGATGGCTGG AATACAGTGC CCATCAGTAA GGGCAGCCGT
    CCTATTGACA CCACTAGGAT CACAAAGATA ACAAAGCTTG GTGCTATTGA TTCAAATCAA
    CTGCTTGCCC CAGGAGGACG TCTGAGTTGG GGTAAAGGAA GCAGCGGAGG AACTGGAGCC
    AAACCAGCAG AAACAGCTCC AGAATCTAGT CGGCCTGCGA CCAGCACCTT AAACAGGTTT
    TCTGCCTTGC AGCAGTCTTC ATCTGCTGAG AGTCAAGATG CTAGACGGCC CTTGCATCGG
    GGAAGTTCTA GTCGGGAACG TTCTGAAAAA GATCGTGGAG ACAGATTTGA TAGAGGTGAA
    AGGTTTGATA AGGGAGAACT GTTTGATAGA TCTGACCGTT CAGATCGAGC TCGTCCTCTT
    CTTACAAAGA GAAGCTATAG CAAGGAAACT GAGGACCGTA GCCGAGAAAG AGAGGCCCAT
    ATACCACAGG AGGCTGTGAG GAAGGTGGCA AGTATGACTG AGCAGCGGGA CCGCAGCAAA
    GAAAGTGTGA AAAAAGAAAT TCCAGCACCG ACAGCAGTGG TTGCCTCATC AAAGCCCTCT
    ATGTCTGAGG ATGAGCTAGA GAAGAAATCT AAAGCCATCA TAGAAGAATA TTTGCACATT
    AATGATATGA AGGAAGCTCT TCAGTGTGTA GTGGAGCTTA ATAGTTCAAC GCTGCTTTTT
    ATCTTTGTGC GGAATGGGAT TGAGTCAACA CTAGAGAGAA GCACTATTGC CAGAGAGCAT
    ATGGGTCTCC TTTTGTACCA GTTAGTAAAG GCAGGAACTC TGTCCAAGGA GCAGTATTAC
    AAAGGGGTGC TTGAAGTTCT TGAAGTAGCA GAAGATATGG AAATAGATAT ACCCCACATC
    TGGCTGTATT TAGCAGAGAT AATCAGCCCT GTGCTGCTAG AGGGGGGGAT TCCGATGGGG
    GAGCTGTTCA GAGAGCTGAC CAAACCTTTA GTGCTCATTG GCAAAGCTGG AGTCCTTCTG
    GCAGAAATCC TTGGATTATT GTGCAAAGGC ATGAGTCATA AGAAGGCTGG AGCCCTGTGG
    AAAGAATCTG GGCTAAGCTG GAAGGAATTC TTGTCCAAGG ACCAGGATAT TAACAAGTTT
    ATAACTGACA AGAAACTCGA ATACACAACA GGAGAGGAGA CTGAAACACC CAGTAAGAAA
    GACCTCACCC CTGCTGAACT TGATAAAATT TTGACAAAAC TTTTGAAAGA GAACAGCAGT
    AACCAGAAGA TATTTGACTG GATTGAAGCC AACTTAAATG ACCAGCAAAC TTCATCCAAC
    ACCTTTGTTC GAACTTTAAT GAGTACTGTG TGCCACTCTG CTATTATTTT TGAGAATCCT
    TGTAGGGTTG ATGTTAATAT TATCCATACT AGAGCCAAAT TTCTTCAGAA ATATTTAAGT
    GACGAGGCCA AGGAACTACA GGCTCTCTAT GCTCTACAGG CACTTGTTGT AAAGCTTGAC
    CAGCCCCCAA ATCTCCTACG GATGTTTTTT GATGCTCTCT ATGATGAGGA TGTGATAAAA
    GAGGATCCAT TCTACAAGTG GGAGTCCAGC AAAGACCCAG CTGAACAGCA AGGAAAAGGT
    GTTGCTCTTA AATCTGTCAC TGCCTTCTTC ACATGGCTAC GGGAAGCAGA GGATGAGTCT
    GATAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa10205
    Clone ID Related Accession (Same CDS sequence) XM_004914401.4 , XM_004914400.3 , XM_004914401.3 , XM_012963378.2 , XM_012963378.3 , XM_004914400.4
    Accession Version XM_012963378.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4809bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963378.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
    CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA
    CAAATGAATA CACAATCTCA GCCTCGACAG CATTATTATC AAAATAGGAC ACAGCCTCCC
    ACTAGTGCCA CCAGAGTGCA GAGTGGTTCT TCTGCTCGAG CAGTTACTCC AGCCCATGTG
    TATCAACCAG GCTCCCAAGT TATGATGATT CCTTCACAGA TTTCATACCC TTCCCAGGGA
    TACTACATTC AGGGACAGGG ACGCTCATAT GTGGTGCCCA CAAACCAGTA CCCTGTGCAG
    ACTGGGGCTC CAGGTTTCTT CCCCAGTGCA AGTCCCTCAG AATATGGCAC ATATGCTGGA
    GCATATTACC CTGCACAACA GACGTTCCCT CCTGGAGTGC CTGGAGCACA AGTTATGATG
    AATCAGCCAC AGGTGATCCC TCCTAAACGG GAGCGCAAAA CGATAAGAAT TAGAGACCCC
    AACCAAGGAG GCAAAGATAT CACAGAAGAA ATTCTCTCAG GAGGAAGAAC ATCCTCTACG
    CCAACGCCTC CACAGTCAAC TGTGGGAAGT TCTGAAATTC AAGCTAATGG AGAAAGCGTT
    CATCATGTTA CCGTTGTTGT GCGCCCAGAT GATGGACCCA AGGCCACCCA AGTGCTGATC
    AAAGCCTCAG AGAGCCCTTC CCCTCCTCTG CCTCTCTCTG AAGCTGAGCC AAGCATAGCA
    AATGTGTGTG ATATTTCAAA AGATAACACT ATCTCTGAGT TGACTTCTCA TATAGTAGAG
    CCCCTAAGTT CAGAAGAGAC AACCATTGAT ACTTCTACCT CTCAGGTGGA TCTCAAACAC
    CAGCCAGTGG CTGCTATGGC AGAGCATGAA ACTCTTATAG AGGAGGCATC AATACAGGAG
    GTGGCCTTTG CAGAGGCACC CATCGCTCAA TCAGAGATAC CAAAATCTCT TGCCTCCCAG
    CCTCTAGCAG AAATCTCTAC CCCTGAACAG ATTTCTAAAC TTGACGCACA AGTGCCATCT
    TTGCAACAAG AGGAGTTACT TACAGAAACT GTTGAACTTA ATTTAAGCAA TCAGGCTCTT
    CCTTCACAAA TTTCTGAGCC TGAACCTTTT GAACCTGAGG CTTCAGCTTC AGATCCTCTA
    CATGAGGAGC CCACAGAGCC CACAGTTTCA ACAGAAGAAC CACAAGCTAA TGGACTGTCT
    GAGGAGACAC CAGTATTACC TGTACAGCCA CAACCAACAT CTGTTTGCCA GACTGAGCCC
    TCCCTAGAAT CCCCCATCGC TCAGCCTGAG GAGCTGCTTC TAACAAATGG AGGTGGACCA
    CCTGGCCTAC AAGAAAATGA TGCACCTGAT TCACAGGTTG AAGCTCATGT TAGCCCAATC
    ACTGAGCCTG AGGACCAACC TGTGACAGTC TCTGATAACG TGCTTGTGGA AGAGGATGTA
    GAAAAAACAT TGCCTGCGGA TACGGAAGTC GTGGCTTCAA CCCAAGTTGT AAAGCAGAGC
    TCCGAGAGTC AGTCTCAGGT TGCTGTTGCT GCACCCAAGA AAAAGCGAAA AATGAAGGAA
    CTAAACAAGA AAGAGGCAGT GGGAGATCTC CTTGATGCGT TCAAAGAGGC TGGTGATGGC
    TCTTCTGATG TGGATTCTTC CTCTAAGCCT ACCAGTGCTG CACCTGAACC AGATCAGAGC
    CCTTGTGCTC CTCCTGAGGA ACAGGATGAA ACTTGGGAAG AGAAGGAAGA TAAACTGGAT
    GCAGAGAATA TTAAACCAGG AGAACATAAA TATAAATACA AAGAAGAGCA ATGGAAACCC
    CTAAACCCGG AGGAAAAGAA GAGTTATGAT CGCGAGTTTC TGCTAGGGTT CCAGTTTATT
    GTGGCCAGTA TGCAGAAACC TGCGGGTCTT CCTCAAATTA CTGATGTGGT GCTGGATAAG
    GCAAACAAAA CTCCATTGCG ACCATTGGAT CCTACACGTC TGACGGGAAT GAACTGTGGG
    CCAGATTTTA CACCTTCATT TGCCAACCTT GGTCGGCCTG CTGTGGGTAA CCGTGGACCA
    CCAGCTGGCA TGGGTCCGAG ACGGTCCCAG CAGGGTCCAC GAAAAGAGCC TCGTAAAATT
    ATTCAAACCG TATCATTAAA TGAGAATGTA CAACTAAACA AAGCTGAAAA GGCATGGAAA
    CCGATAAACA AGAGAGCTGG CGATGAGGAG GACCCAGAAA ATATTAAGAC ACAGGACCTA
    TTCCGAAGGG TTCGCAGTAT CTTGAATAAG CTGACCCCTC AGATGTTCCA GCAGCTGATG
    AAGCAGGTCA CTGACCTTAC TATTGATACC GAAGATCGTC TTAAGGGTGT CATTGACCTA
    ATTTTTGAAA AAGCCATTTC AGAACCCAAT TTCTCTGTTG CATATGCCAA CATGTGTCGT
    TGCCTTATGG GGCTTAAAGT TCCAACCACA GACAAGCCAG GGGTTACAGT CAACTTCCGC
    AAGTTGTTAC TGAATCGGTG CCAGAAGGAG TTTGAGAAGG ATAAGGATGA TGATGAAGTG
    TTTGAGAAGA AACAAAAAGA AATGGATGCT GCTGCTTCGC CGGAGGAAAA AGCACGTTTG
    AATGATGAAC TGGTCGAGGC CCGTGACAGT GCACGACGGC GATCCTTGGG GAACATTAAG
    TTCATTGGAG AACTGTTTAA GTTAAAAATG CTGACTGAAG CTATAATGCA CGATTGTGTG
    GTGAAGCTAT TAAAAAATCA TGATGAGGAA TCTCTGGAGT GCCTCTGCCG ACTGCTGTCC
    ACCATTGGCA AGGACCTGGA TTTTGAGAGA GCTAAGCCAC GTATGGACCA GTACTTCAAC
    CAGATGGATA AAATCATTAA GGAGCGGAAG ACATCTTCAC GAATTAGATT CATGCTGCAG
    GATGTGATTG ATTTGCGTCT GTGTAATTGG GTGCCTCGTC GTGCAGATCA GGGCCCTAAA
    ACTATAGATC AGATTCATAA AGAAGCAGAA ATGGAAAAGC AGAGGGAGCA GGTTAAGGTG
    CAACAACTGA TGTCTAAACA AGACAAGCGG CGAGAGCCCC CAGGACGACC CACTGGAAGC
    CGGGGTAACC AGCCTGCAGA TGATGGCTGG AATACAGTGC CCATCAGTAA GGGCAGCCGT
    CCTATTGACA CCACTAGGAT CACAAAGATA ACAAAGCTTG GTGCTATTGA TTCAAATCAA
    CTGCTTGCCC CAGGAGGACG TCTGAGTTGG GGTAAAGGAA GCAGCGGAGG AACTGGAGCC
    AAACCAGCAG AAACAGCTCC AGAATCTAGT CGGCCTGCGA CCAGCACCTT AAACAGGTTT
    TCTGCCTTGC AGCAGTCTTC ATCTGCTGAG AGTCAAGATG CTAGACGGCC CTTGCATCGG
    GGAAGTTCTA GTCGGGAACG TTCTGAAAAA GATCGTGGAG ACAGATTTGA TAGAGGTGAA
    AGGTTTGATA AGGGAGAACT GTTTGATAGA TCTGACCGTT CAGATCGAGC TCGTCCTCTT
    CTTACAAAGA GAAGCTATAG CAAGGAAACT GAGGACCGTA GCCGAGAAAG AGAGGCCCAT
    ATACCACAGG AGGCTGTGAG GAAGGTGGCA AGTATGACTG AGCAGCGGGA CCGCAGCAAA
    GAAAGTGTGA AAAAAGAAAT TCCAGCACCG ACAGCAGTGG TTGCCTCATC AAAGCCCTCT
    ATGTCTGAGG ATGAGCTAGA GAAGAAATCT AAAGCCATCA TAGAAGAATA TTTGCACATT
    AATGATATGA AGGAAGCTCT TCAGTGTGTA GTGGAGCTTA ATAGTTCAAC GCTGCTTTTT
    ATCTTTGTGC GGAATGGGAT TGAGTCAACA CTAGAGAGAA GCACTATTGC CAGAGAGCAT
    ATGGGTCTCC TTTTGTACCA GTTAGTAAAG GCAGGAACTC TGTCCAAGGA GCAGTATTAC
    AAAGGGGTGC TTGAAGTTCT TGAAGTAGCA GAAGATATGG AAATAGATAT ACCCCACATC
    TGGCTGTATT TAGCAGAGAT AATCAGCCCT GTGCTGCTAG AGGGGGGGAT TCCGATGGGG
    GAGCTGTTCA GAGAGCTGAC CAAACCTTTA GTGCTCATTG GCAAAGCTGG AGTCCTTCTG
    GCAGAAATCC TTGGATTATT GTGCAAAGGC ATGAGTCATA AGAAGGCTGG AGCCCTGTGG
    AAAGAATCTG GGCTAAGCTG GAAGGAATTC TTGTCCAAGG ACCAGGATAT TAACAAGTTT
    ATAACTGACA AGAAACTCGA ATACACAACA GGAGAGGAGA CTGAAACACC CAGTAAGAAA
    GACCTCACCC CTGCTGAACT TGATAAAATT TTGACAAAAC TTTTGAAAGA GAACAGCAGT
    AACCAGAAGA TATTTGACTG GATTGAAGCC AACTTAAATG ACCAGCAAAC TTCATCCAAC
    ACCTTTGTTC GAACTTTAAT GAGTACTGTG TGCCACTCTG CTATTATTTT TGAGAATCCT
    TGTAGGGTTG ATGTTAATAT TATCCATACT AGAGCCAAAT TTCTTCAGAA ATATTTAAGT
    GACGAGGCCA AGGAACTACA GGCTCTCTAT GCTCTACAGG CACTTGTTGT AAAGCTTGAC
    CAGCCCCCAA ATCTCCTACG GATGTTTTTT GATGCTCTCT ATGATGAGGA TGTGATAAAA
    GAGGATCCAT TCTACAAGTG GGAGTCCAGC AAAGACCCAG CTGAACAGCA AGGAAAAGGT
    GTTGCTCTTA AATCTGTCAC TGCCTTCTTC ACATGGCTAC GGGAAGCAGA GGATGAGTCT
    GATAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012818832.1
    CDS343..5151
    Translation

    Target ORF information:

    RefSeq Version XM_012963378.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012963378.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
    CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA
    CAAATGAATA CACAATCTCA GCCTCGACAG CATTATTATC AAAATAGGAC ACAGCCTCCC
    ACTAGTGCCA CCAGAGTGCA GAGTGGTTCT TCTGCTCGAG CAGTTACTCC AGCCCATGTG
    TATCAACCAG GCTCCCAAGT TATGATGATT CCTTCACAGA TTTCATACCC TTCCCAGGGA
    TACTACATTC AGGGACAGGG ACGCTCATAT GTGGTGCCCA CAAACCAGTA CCCTGTGCAG
    ACTGGGGCTC CAGGTTTCTT CCCCAGTGCA AGTCCCTCAG AATATGGCAC ATATGCTGGA
    GCATATTACC CTGCACAACA GACGTTCCCT CCTGGAGTGC CTGGAGCACA AGTTATGATG
    AATCAGCCAC AGGTGATCCC TCCTAAACGG GAGCGCAAAA CGATAAGAAT TAGAGACCCC
    AACCAAGGAG GCAAAGATAT CACAGAAGAA ATTCTCTCAG GAGGAAGAAC ATCCTCTACG
    CCAACGCCTC CACAGTCAAC TGTGGGAAGT TCTGAAATTC AAGCTAATGG AGAAAGCGTT
    CATCATGTTA CCGTTGTTGT GCGCCCAGAT GATGGACCCA AGGCCACCCA AGTGCTGATC
    AAAGCCTCAG AGAGCCCTTC CCCTCCTCTG CCTCTCTCTG AAGCTGAGCC AAGCATAGCA
    AATGTGTGTG ATATTTCAAA AGATAACACT ATCTCTGAGT TGACTTCTCA TATAGTAGAG
    CCCCTAAGTT CAGAAGAGAC AACCATTGAT ACTTCTACCT CTCAGGTGGA TCTCAAACAC
    CAGCCAGTGG CTGCTATGGC AGAGCATGAA ACTCTTATAG AGGAGGCATC AATACAGGAG
    GTGGCCTTTG CAGAGGCACC CATCGCTCAA TCAGAGATAC CAAAATCTCT TGCCTCCCAG
    CCTCTAGCAG AAATCTCTAC CCCTGAACAG ATTTCTAAAC TTGACGCACA AGTGCCATCT
    TTGCAACAAG AGGAGTTACT TACAGAAACT GTTGAACTTA ATTTAAGCAA TCAGGCTCTT
    CCTTCACAAA TTTCTGAGCC TGAACCTTTT GAACCTGAGG CTTCAGCTTC AGATCCTCTA
    CATGAGGAGC CCACAGAGCC CACAGTTTCA ACAGAAGAAC CACAAGCTAA TGGACTGTCT
    GAGGAGACAC CAGTATTACC TGTACAGCCA CAACCAACAT CTGTTTGCCA GACTGAGCCC
    TCCCTAGAAT CCCCCATCGC TCAGCCTGAG GAGCTGCTTC TAACAAATGG AGGTGGACCA
    CCTGGCCTAC AAGAAAATGA TGCACCTGAT TCACAGGTTG AAGCTCATGT TAGCCCAATC
    ACTGAGCCTG AGGACCAACC TGTGACAGTC TCTGATAACG TGCTTGTGGA AGAGGATGTA
    GAAAAAACAT TGCCTGCGGA TACGGAAGTC GTGGCTTCAA CCCAAGTTGT AAAGCAGAGC
    TCCGAGAGTC AGTCTCAGGT TGCTGTTGCT GCACCCAAGA AAAAGCGAAA AATGAAGGAA
    CTAAACAAGA AAGAGGCAGT GGGAGATCTC CTTGATGCGT TCAAAGAGGC TGGTGATGGC
    TCTTCTGATG TGGATTCTTC CTCTAAGCCT ACCAGTGCTG CACCTGAACC AGATCAGAGC
    CCTTGTGCTC CTCCTGAGGA ACAGGATGAA ACTTGGGAAG AGAAGGAAGA TAAACTGGAT
    GCAGAGAATA TTAAACCAGG AGAACATAAA TATAAATACA AAGAAGAGCA ATGGAAACCC
    CTAAACCCGG AGGAAAAGAA GAGTTATGAT CGCGAGTTTC TGCTAGGGTT CCAGTTTATT
    GTGGCCAGTA TGCAGAAACC TGCGGGTCTT CCTCAAATTA CTGATGTGGT GCTGGATAAG
    GCAAACAAAA CTCCATTGCG ACCATTGGAT CCTACACGTC TGACGGGAAT GAACTGTGGG
    CCAGATTTTA CACCTTCATT TGCCAACCTT GGTCGGCCTG CTGTGGGTAA CCGTGGACCA
    CCAGCTGGCA TGGGTCCGAG ACGGTCCCAG CAGGGTCCAC GAAAAGAGCC TCGTAAAATT
    ATTCAAACCG TATCATTAAA TGAGAATGTA CAACTAAACA AAGCTGAAAA GGCATGGAAA
    CCGATAAACA AGAGAGCTGG CGATGAGGAG GACCCAGAAA ATATTAAGAC ACAGGACCTA
    TTCCGAAGGG TTCGCAGTAT CTTGAATAAG CTGACCCCTC AGATGTTCCA GCAGCTGATG
    AAGCAGGTCA CTGACCTTAC TATTGATACC GAAGATCGTC TTAAGGGTGT CATTGACCTA
    ATTTTTGAAA AAGCCATTTC AGAACCCAAT TTCTCTGTTG CATATGCCAA CATGTGTCGT
    TGCCTTATGG GGCTTAAAGT TCCAACCACA GACAAGCCAG GGGTTACAGT CAACTTCCGC
    AAGTTGTTAC TGAATCGGTG CCAGAAGGAG TTTGAGAAGG ATAAGGATGA TGATGAAGTG
    TTTGAGAAGA AACAAAAAGA AATGGATGCT GCTGCTTCGC CGGAGGAAAA AGCACGTTTG
    AATGATGAAC TGGTCGAGGC CCGTGACAGT GCACGACGGC GATCCTTGGG GAACATTAAG
    TTCATTGGAG AACTGTTTAA GTTAAAAATG CTGACTGAAG CTATAATGCA CGATTGTGTG
    GTGAAGCTAT TAAAAAATCA TGATGAGGAA TCTCTGGAGT GCCTCTGCCG ACTGCTGTCC
    ACCATTGGCA AGGACCTGGA TTTTGAGAGA GCTAAGCCAC GTATGGACCA GTACTTCAAC
    CAGATGGATA AAATCATTAA GGAGCGGAAG ACATCTTCAC GAATTAGATT CATGCTGCAG
    GATGTGATTG ATTTGCGTCT GTGTAATTGG GTGCCTCGTC GTGCAGATCA GGGCCCTAAA
    ACTATAGATC AGATTCATAA AGAAGCAGAA ATGGAAAAGC AGAGGGAGCA GGTTAAGGTG
    CAACAACTGA TGTCTAAACA AGACAAGCGG CGAGAGCCCC CAGGACGACC CACTGGAAGC
    CGGGGTAACC AGCCTGCAGA TGATGGCTGG AATACAGTGC CCATCAGTAA GGGCAGCCGT
    CCTATTGACA CCACTAGGAT CACAAAGATA ACAAAGCTTG GTGCTATTGA TTCAAATCAA
    CTGCTTGCCC CAGGAGGACG TCTGAGTTGG GGTAAAGGAA GCAGCGGAGG AACTGGAGCC
    AAACCAGCAG AAACAGCTCC AGAATCTAGT CGGCCTGCGA CCAGCACCTT AAACAGGTTT
    TCTGCCTTGC AGCAGTCTTC ATCTGCTGAG AGTCAAGATG CTAGACGGCC CTTGCATCGG
    GGAAGTTCTA GTCGGGAACG TTCTGAAAAA GATCGTGGAG ACAGATTTGA TAGAGGTGAA
    AGGTTTGATA AGGGAGAACT GTTTGATAGA TCTGACCGTT CAGATCGAGC TCGTCCTCTT
    CTTACAAAGA GAAGCTATAG CAAGGAAACT GAGGACCGTA GCCGAGAAAG AGAGGCCCAT
    ATACCACAGG AGGCTGTGAG GAAGGTGGCA AGTATGACTG AGCAGCGGGA CCGCAGCAAA
    GAAAGTGTGA AAAAAGAAAT TCCAGCACCG ACAGCAGTGG TTGCCTCATC AAAGCCCTCT
    ATGTCTGAGG ATGAGCTAGA GAAGAAATCT AAAGCCATCA TAGAAGAATA TTTGCACATT
    AATGATATGA AGGAAGCTCT TCAGTGTGTA GTGGAGCTTA ATAGTTCAAC GCTGCTTTTT
    ATCTTTGTGC GGAATGGGAT TGAGTCAACA CTAGAGAGAA GCACTATTGC CAGAGAGCAT
    ATGGGTCTCC TTTTGTACCA GTTAGTAAAG GCAGGAACTC TGTCCAAGGA GCAGTATTAC
    AAAGGGGTGC TTGAAGTTCT TGAAGTAGCA GAAGATATGG AAATAGATAT ACCCCACATC
    TGGCTGTATT TAGCAGAGAT AATCAGCCCT GTGCTGCTAG AGGGGGGGAT TCCGATGGGG
    GAGCTGTTCA GAGAGCTGAC CAAACCTTTA GTGCTCATTG GCAAAGCTGG AGTCCTTCTG
    GCAGAAATCC TTGGATTATT GTGCAAAGGC ATGAGTCATA AGAAGGCTGG AGCCCTGTGG
    AAAGAATCTG GGCTAAGCTG GAAGGAATTC TTGTCCAAGG ACCAGGATAT TAACAAGTTT
    ATAACTGACA AGAAACTCGA ATACACAACA GGAGAGGAGA CTGAAACACC CAGTAAGAAA
    GACCTCACCC CTGCTGAACT TGATAAAATT TTGACAAAAC TTTTGAAAGA GAACAGCAGT
    AACCAGAAGA TATTTGACTG GATTGAAGCC AACTTAAATG ACCAGCAAAC TTCATCCAAC
    ACCTTTGTTC GAACTTTAAT GAGTACTGTG TGCCACTCTG CTATTATTTT TGAGAATCCT
    TGTAGGGTTG ATGTTAATAT TATCCATACT AGAGCCAAAT TTCTTCAGAA ATATTTAAGT
    GACGAGGCCA AGGAACTACA GGCTCTCTAT GCTCTACAGG CACTTGTTGT AAAGCTTGAC
    CAGCCCCCAA ATCTCCTACG GATGTTTTTT GATGCTCTCT ATGATGAGGA TGTGATAAAA
    GAGGATCCAT TCTACAAGTG GGAGTCCAGC AAAGACCCAG CTGAACAGCA AGGAAAAGGT
    GTTGCTCTTA AATCTGTCAC TGCCTTCTTC ACATGGCTAC GGGAAGCAGA GGATGAGTCT
    GATAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa10206
    Clone ID Related Accession (Same CDS sequence) XM_004914402.3 , XM_004914402.4
    Accession Version XM_004914402.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4641bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004914402.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
    CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA
    CAAATGAATA CACAATCTCA GCCTCGACAG GGACGCTCAT ATGTGGTGCC CACAAACCAG
    TACCCTGTGC AGACTGGGGC TCCAGGTTTC TTCCCCAGTG CAAGTCCCTC AGAATATGGC
    ACATATGCTG GAGCATATTA CCCTGCACAA CAGACGTTCC CTCCTGGAGT GCCTGGAGCA
    CAAGTTATGA TGAATCAGCC ACAGGTGATC CCTCCTAAAC GGGAGCGCAA AACGATAAGA
    ATTAGAGACC CCAACCAAGG AGGCAAAGAT ATCACAGAAG AAATTCTCTC AGGAGGAAGA
    ACATCCTCTA CGCCAACGCC TCCACAGTCA ACTGTGGGAA GTTCTGAAAT TCAAGCTAAT
    GGAGAAAGCG TTCATCATGT TACCGTTGTT GTGCGCCCAG ATGATGGACC CAAGGCCACC
    CAAGTGCTGA TCAAAGCCTC AGAGAGCCCT TCCCCTCCTC TGCCTCTCTC TGAAGCTGAG
    CCAAGCATAG CAAATGTGTG TGATATTTCA AAAGATAACA CTATCTCTGA GTTGACTTCT
    CATATAGTAG AGCCCCTAAG TTCAGAAGAG ACAACCATTG ATACTTCTAC CTCTCAGGTG
    GATCTCAAAC ACCAGCCAGT GGCTGCTATG GCAGAGCATG AAACTCTTAT AGAGGAGGCA
    TCAATACAGG AGGTGGCCTT TGCAGAGGCA CCCATCGCTC AATCAGAGAT ACCAAAATCT
    CTTGCCTCCC AGCCTCTAGC AGAAATCTCT ACCCCTGAAC AGATTTCTAA ACTTGACGCA
    CAAGTGCCAT CTTTGCAACA AGAGGAGTTA CTTACAGAAA CTGTTGAACT TAATTTAAGC
    AATCAGGCTC TTCCTTCACA AATTTCTGAG CCTGAACCTT TTGAACCTGA GGCTTCAGCT
    TCAGATCCTC TACATGAGGA GCCCACAGAG CCCACAGTTT CAACAGAAGA ACCACAAGCT
    AATGGACTGT CTGAGGAGAC ACCAGTATTA CCTGTACAGC CACAACCAAC ATCTGTTTGC
    CAGACTGAGC CCTCCCTAGA ATCCCCCATC GCTCAGCCTG AGGAGCTGCT TCTAACAAAT
    GGAGGTGGAC CACCTGGCCT ACAAGAAAAT GATGCACCTG ATTCACAGGT TGAAGCTCAT
    GTTAGCCCAA TCACTGAGCC TGAGGACCAA CCTGTGACAG TCTCTGATAA CGTGCTTGTG
    GAAGAGGATG TAGAAAAAAC ATTGCCTGCG GATACGGAAG TCGTGGCTTC AACCCAAGTT
    GTAAAGCAGA GCTCCGAGAG TCAGTCTCAG GTTGCTGTTG CTGCACCCAA GAAAAAGCGA
    AAAATGAAGG AACTAAACAA GAAAGAGGCA GTGGGAGATC TCCTTGATGC GTTCAAAGAG
    GCTGGTGATG GCTCTTCTGA TGTGGATTCT TCCTCTAAGC CTACCAGTGC TGCACCTGAA
    CCAGATCAGA GCCCTTGTGC TCCTCCTGAG GAACAGGATG AAACTTGGGA AGAGAAGGAA
    GATAAACTGG ATGCAGAGAA TATTAAACCA GGAGAACATA AATATAAATA CAAAGAAGAG
    CAATGGAAAC CCCTAAACCC GGAGGAAAAG AAGAGTTATG ATCGCGAGTT TCTGCTAGGG
    TTCCAGTTTA TTGTGGCCAG TATGCAGAAA CCTGCGGGTC TTCCTCAAAT TACTGATGTG
    GTGCTGGATA AGGCAAACAA AACTCCATTG CGACCATTGG ATCCTACACG TCTGACGGGA
    ATGAACTGTG GGCCAGATTT TACACCTTCA TTTGCCAACC TTGGTCGGCC TGCTGTGGGT
    AACCGTGGAC CACCAGCTGG CATGGGTCCG AGACGGTCCC AGCAGGGTCC ACGAAAAGAG
    CCTCGTAAAA TTATTCAAAC CGTATCATTA AATGAGAATG TACAACTAAA CAAAGCTGAA
    AAGGCATGGA AACCGATAAA CAAGAGAGCT GGCGATGAGG AGGACCCAGA AAATATTAAG
    ACACAGGACC TATTCCGAAG GGTTCGCAGT ATCTTGAATA AGCTGACCCC TCAGATGTTC
    CAGCAGCTGA TGAAGCAGGT CACTGACCTT ACTATTGATA CCGAAGATCG TCTTAAGGGT
    GTCATTGACC TAATTTTTGA AAAAGCCATT TCAGAACCCA ATTTCTCTGT TGCATATGCC
    AACATGTGTC GTTGCCTTAT GGGGCTTAAA GTTCCAACCA CAGACAAGCC AGGGGTTACA
    GTCAACTTCC GCAAGTTGTT ACTGAATCGG TGCCAGAAGG AGTTTGAGAA GGATAAGGAT
    GATGATGAAG TGTTTGAGAA GAAACAAAAA GAAATGGATG CTGCTGCTTC GCCGGAGGAA
    AAAGCACGTT TGAATGATGA ACTGGTCGAG GCCCGTGACA GTGCACGACG GCGATCCTTG
    GGGAACATTA AGTTCATTGG AGAACTGTTT AAGTTAAAAA TGCTGACTGA AGCTATAATG
    CACGATTGTG TGGTGAAGCT ATTAAAAAAT CATGATGAGG AATCTCTGGA GTGCCTCTGC
    CGACTGCTGT CCACCATTGG CAAGGACCTG GATTTTGAGA GAGCTAAGCC ACGTATGGAC
    CAGTACTTCA ACCAGATGGA TAAAATCATT AAGGAGCGGA AGACATCTTC ACGAATTAGA
    TTCATGCTGC AGGATGTGAT TGATTTGCGT CTGTGTAATT GGGTGCCTCG TCGTGCAGAT
    CAGGGCCCTA AAACTATAGA TCAGATTCAT AAAGAAGCAG AAATGGAAAA GCAGAGGGAG
    CAGGTTAAGG TGCAACAACT GATGTCTAAA CAAGACAAGC GGCGAGAGCC CCCAGGACGA
    CCCACTGGAA GCCGGGGTAA CCAGCCTGCA GATGATGGCT GGAATACAGT GCCCATCAGT
    AAGGGCAGCC GTCCTATTGA CACCACTAGG ATCACAAAGA TAACAAAGCT TGGTGCTATT
    GATTCAAATC AACTGCTTGC CCCAGGAGGA CGTCTGAGTT GGGGTAAAGG AAGCAGCGGA
    GGAACTGGAG CCAAACCAGC AGAAACAGCT CCAGAATCTA GTCGGCCTGC GACCAGCACC
    TTAAACAGGT TTTCTGCCTT GCAGCAGTCT TCATCTGCTG AGAGTCAAGA TGCTAGACGG
    CCCTTGCATC GGGGAAGTTC TAGTCGGGAA CGTTCTGAAA AAGATCGTGG AGACAGATTT
    GATAGAGGTG AAAGGTTTGA TAAGGGAGAA CTGTTTGATA GATCTGACCG TTCAGATCGA
    GCTCGTCCTC TTCTTACAAA GAGAAGCTAT AGCAAGGAAA CTGAGGACCG TAGCCGAGAA
    AGAGAGGCCC ATATACCACA GGAGGCTGTG AGGAAGGTGG CAAGTATGAC TGAGCAGCGG
    GACCGCAGCA AAGAAAGTGT GAAAAAAGAA ATTCCAGCAC CGACAGCAGT GGTTGCCTCA
    TCAAAGCCCT CTATGTCTGA GGATGAGCTA GAGAAGAAAT CTAAAGCCAT CATAGAAGAA
    TATTTGCACA TTAATGATAT GAAGGAAGCT CTTCAGTGTG TAGTGGAGCT TAATAGTTCA
    ACGCTGCTTT TTATCTTTGT GCGGAATGGG ATTGAGTCAA CACTAGAGAG AAGCACTATT
    GCCAGAGAGC ATATGGGTCT CCTTTTGTAC CAGTTAGTAA AGGCAGGAAC TCTGTCCAAG
    GAGCAGTATT ACAAAGGGGT GCTTGAAGTT CTTGAAGTAG CAGAAGATAT GGAAATAGAT
    ATACCCCACA TCTGGCTGTA TTTAGCAGAG ATAATCAGCC CTGTGCTGCT AGAGGGGGGG
    ATTCCGATGG GGGAGCTGTT CAGAGAGCTG ACCAAACCTT TAGTGCTCAT TGGCAAAGCT
    GGAGTCCTTC TGGCAGAAAT CCTTGGATTA TTGTGCAAAG GCATGAGTCA TAAGAAGGCT
    GGAGCCCTGT GGAAAGAATC TGGGCTAAGC TGGAAGGAAT TCTTGTCCAA GGACCAGGAT
    ATTAACAAGT TTATAACTGA CAAGAAACTC GAATACACAA CAGGAGAGGA GACTGAAACA
    CCCAGTAAGA AAGACCTCAC CCCTGCTGAA CTTGATAAAA TTTTGACAAA ACTTTTGAAA
    GAGAACAGCA GTAACCAGAA GATATTTGAC TGGATTGAAG CCAACTTAAA TGACCAGCAA
    ACTTCATCCA ACACCTTTGT TCGAACTTTA ATGAGTACTG TGTGCCACTC TGCTATTATT
    TTTGAGAATC CTTGTAGGGT TGATGTTAAT ATTATCCATA CTAGAGCCAA ATTTCTTCAG
    AAATATTTAA GTGACGAGGC CAAGGAACTA CAGGCTCTCT ATGCTCTACA GGCACTTGTT
    GTAAAGCTTG ACCAGCCCCC AAATCTCCTA CGGATGTTTT TTGATGCTCT CTATGATGAG
    GATGTGATAA AAGAGGATCC ATTCTACAAG TGGGAGTCCA GCAAAGACCC AGCTGAACAG
    CAAGGAAAAG GTGTTGCTCT TAAATCTGTC ACTGCCTTCT TCACATGGCT ACGGGAAGCA
    GAGGATGAGT CTGATAATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004914459.1
    CDS216..4856
    Translation

    Target ORF information:

    RefSeq Version XM_004914402.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004914402.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
    CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA
    CAAATGAATA CACAATCTCA GCCTCGACAG GGACGCTCAT ATGTGGTGCC CACAAACCAG
    TACCCTGTGC AGACTGGGGC TCCAGGTTTC TTCCCCAGTG CAAGTCCCTC AGAATATGGC
    ACATATGCTG GAGCATATTA CCCTGCACAA CAGACGTTCC CTCCTGGAGT GCCTGGAGCA
    CAAGTTATGA TGAATCAGCC ACAGGTGATC CCTCCTAAAC GGGAGCGCAA AACGATAAGA
    ATTAGAGACC CCAACCAAGG AGGCAAAGAT ATCACAGAAG AAATTCTCTC AGGAGGAAGA
    ACATCCTCTA CGCCAACGCC TCCACAGTCA ACTGTGGGAA GTTCTGAAAT TCAAGCTAAT
    GGAGAAAGCG TTCATCATGT TACCGTTGTT GTGCGCCCAG ATGATGGACC CAAGGCCACC
    CAAGTGCTGA TCAAAGCCTC AGAGAGCCCT TCCCCTCCTC TGCCTCTCTC TGAAGCTGAG
    CCAAGCATAG CAAATGTGTG TGATATTTCA AAAGATAACA CTATCTCTGA GTTGACTTCT
    CATATAGTAG AGCCCCTAAG TTCAGAAGAG ACAACCATTG ATACTTCTAC CTCTCAGGTG
    GATCTCAAAC ACCAGCCAGT GGCTGCTATG GCAGAGCATG AAACTCTTAT AGAGGAGGCA
    TCAATACAGG AGGTGGCCTT TGCAGAGGCA CCCATCGCTC AATCAGAGAT ACCAAAATCT
    CTTGCCTCCC AGCCTCTAGC AGAAATCTCT ACCCCTGAAC AGATTTCTAA ACTTGACGCA
    CAAGTGCCAT CTTTGCAACA AGAGGAGTTA CTTACAGAAA CTGTTGAACT TAATTTAAGC
    AATCAGGCTC TTCCTTCACA AATTTCTGAG CCTGAACCTT TTGAACCTGA GGCTTCAGCT
    TCAGATCCTC TACATGAGGA GCCCACAGAG CCCACAGTTT CAACAGAAGA ACCACAAGCT
    AATGGACTGT CTGAGGAGAC ACCAGTATTA CCTGTACAGC CACAACCAAC ATCTGTTTGC
    CAGACTGAGC CCTCCCTAGA ATCCCCCATC GCTCAGCCTG AGGAGCTGCT TCTAACAAAT
    GGAGGTGGAC CACCTGGCCT ACAAGAAAAT GATGCACCTG ATTCACAGGT TGAAGCTCAT
    GTTAGCCCAA TCACTGAGCC TGAGGACCAA CCTGTGACAG TCTCTGATAA CGTGCTTGTG
    GAAGAGGATG TAGAAAAAAC ATTGCCTGCG GATACGGAAG TCGTGGCTTC AACCCAAGTT
    GTAAAGCAGA GCTCCGAGAG TCAGTCTCAG GTTGCTGTTG CTGCACCCAA GAAAAAGCGA
    AAAATGAAGG AACTAAACAA GAAAGAGGCA GTGGGAGATC TCCTTGATGC GTTCAAAGAG
    GCTGGTGATG GCTCTTCTGA TGTGGATTCT TCCTCTAAGC CTACCAGTGC TGCACCTGAA
    CCAGATCAGA GCCCTTGTGC TCCTCCTGAG GAACAGGATG AAACTTGGGA AGAGAAGGAA
    GATAAACTGG ATGCAGAGAA TATTAAACCA GGAGAACATA AATATAAATA CAAAGAAGAG
    CAATGGAAAC CCCTAAACCC GGAGGAAAAG AAGAGTTATG ATCGCGAGTT TCTGCTAGGG
    TTCCAGTTTA TTGTGGCCAG TATGCAGAAA CCTGCGGGTC TTCCTCAAAT TACTGATGTG
    GTGCTGGATA AGGCAAACAA AACTCCATTG CGACCATTGG ATCCTACACG TCTGACGGGA
    ATGAACTGTG GGCCAGATTT TACACCTTCA TTTGCCAACC TTGGTCGGCC TGCTGTGGGT
    AACCGTGGAC CACCAGCTGG CATGGGTCCG AGACGGTCCC AGCAGGGTCC ACGAAAAGAG
    CCTCGTAAAA TTATTCAAAC CGTATCATTA AATGAGAATG TACAACTAAA CAAAGCTGAA
    AAGGCATGGA AACCGATAAA CAAGAGAGCT GGCGATGAGG AGGACCCAGA AAATATTAAG
    ACACAGGACC TATTCCGAAG GGTTCGCAGT ATCTTGAATA AGCTGACCCC TCAGATGTTC
    CAGCAGCTGA TGAAGCAGGT CACTGACCTT ACTATTGATA CCGAAGATCG TCTTAAGGGT
    GTCATTGACC TAATTTTTGA AAAAGCCATT TCAGAACCCA ATTTCTCTGT TGCATATGCC
    AACATGTGTC GTTGCCTTAT GGGGCTTAAA GTTCCAACCA CAGACAAGCC AGGGGTTACA
    GTCAACTTCC GCAAGTTGTT ACTGAATCGG TGCCAGAAGG AGTTTGAGAA GGATAAGGAT
    GATGATGAAG TGTTTGAGAA GAAACAAAAA GAAATGGATG CTGCTGCTTC GCCGGAGGAA
    AAAGCACGTT TGAATGATGA ACTGGTCGAG GCCCGTGACA GTGCACGACG GCGATCCTTG
    GGGAACATTA AGTTCATTGG AGAACTGTTT AAGTTAAAAA TGCTGACTGA AGCTATAATG
    CACGATTGTG TGGTGAAGCT ATTAAAAAAT CATGATGAGG AATCTCTGGA GTGCCTCTGC
    CGACTGCTGT CCACCATTGG CAAGGACCTG GATTTTGAGA GAGCTAAGCC ACGTATGGAC
    CAGTACTTCA ACCAGATGGA TAAAATCATT AAGGAGCGGA AGACATCTTC ACGAATTAGA
    TTCATGCTGC AGGATGTGAT TGATTTGCGT CTGTGTAATT GGGTGCCTCG TCGTGCAGAT
    CAGGGCCCTA AAACTATAGA TCAGATTCAT AAAGAAGCAG AAATGGAAAA GCAGAGGGAG
    CAGGTTAAGG TGCAACAACT GATGTCTAAA CAAGACAAGC GGCGAGAGCC CCCAGGACGA
    CCCACTGGAA GCCGGGGTAA CCAGCCTGCA GATGATGGCT GGAATACAGT GCCCATCAGT
    AAGGGCAGCC GTCCTATTGA CACCACTAGG ATCACAAAGA TAACAAAGCT TGGTGCTATT
    GATTCAAATC AACTGCTTGC CCCAGGAGGA CGTCTGAGTT GGGGTAAAGG AAGCAGCGGA
    GGAACTGGAG CCAAACCAGC AGAAACAGCT CCAGAATCTA GTCGGCCTGC GACCAGCACC
    TTAAACAGGT TTTCTGCCTT GCAGCAGTCT TCATCTGCTG AGAGTCAAGA TGCTAGACGG
    CCCTTGCATC GGGGAAGTTC TAGTCGGGAA CGTTCTGAAA AAGATCGTGG AGACAGATTT
    GATAGAGGTG AAAGGTTTGA TAAGGGAGAA CTGTTTGATA GATCTGACCG TTCAGATCGA
    GCTCGTCCTC TTCTTACAAA GAGAAGCTAT AGCAAGGAAA CTGAGGACCG TAGCCGAGAA
    AGAGAGGCCC ATATACCACA GGAGGCTGTG AGGAAGGTGG CAAGTATGAC TGAGCAGCGG
    GACCGCAGCA AAGAAAGTGT GAAAAAAGAA ATTCCAGCAC CGACAGCAGT GGTTGCCTCA
    TCAAAGCCCT CTATGTCTGA GGATGAGCTA GAGAAGAAAT CTAAAGCCAT CATAGAAGAA
    TATTTGCACA TTAATGATAT GAAGGAAGCT CTTCAGTGTG TAGTGGAGCT TAATAGTTCA
    ACGCTGCTTT TTATCTTTGT GCGGAATGGG ATTGAGTCAA CACTAGAGAG AAGCACTATT
    GCCAGAGAGC ATATGGGTCT CCTTTTGTAC CAGTTAGTAA AGGCAGGAAC TCTGTCCAAG
    GAGCAGTATT ACAAAGGGGT GCTTGAAGTT CTTGAAGTAG CAGAAGATAT GGAAATAGAT
    ATACCCCACA TCTGGCTGTA TTTAGCAGAG ATAATCAGCC CTGTGCTGCT AGAGGGGGGG
    ATTCCGATGG GGGAGCTGTT CAGAGAGCTG ACCAAACCTT TAGTGCTCAT TGGCAAAGCT
    GGAGTCCTTC TGGCAGAAAT CCTTGGATTA TTGTGCAAAG GCATGAGTCA TAAGAAGGCT
    GGAGCCCTGT GGAAAGAATC TGGGCTAAGC TGGAAGGAAT TCTTGTCCAA GGACCAGGAT
    ATTAACAAGT TTATAACTGA CAAGAAACTC GAATACACAA CAGGAGAGGA GACTGAAACA
    CCCAGTAAGA AAGACCTCAC CCCTGCTGAA CTTGATAAAA TTTTGACAAA ACTTTTGAAA
    GAGAACAGCA GTAACCAGAA GATATTTGAC TGGATTGAAG CCAACTTAAA TGACCAGCAA
    ACTTCATCCA ACACCTTTGT TCGAACTTTA ATGAGTACTG TGTGCCACTC TGCTATTATT
    TTTGAGAATC CTTGTAGGGT TGATGTTAAT ATTATCCATA CTAGAGCCAA ATTTCTTCAG
    AAATATTTAA GTGACGAGGC CAAGGAACTA CAGGCTCTCT ATGCTCTACA GGCACTTGTT
    GTAAAGCTTG ACCAGCCCCC AAATCTCCTA CGGATGTTTT TTGATGCTCT CTATGATGAG
    GATGTGATAA AAGAGGATCC ATTCTACAAG TGGGAGTCCA GCAAAGACCC AGCTGAACAG
    CAAGGAAAAG GTGTTGCTCT TAAATCTGTC ACTGCCTTCT TCACATGGCT ACGGGAAGCA
    GAGGATGAGT CTGATAATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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