prpf8 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

  • Gene

  • Clones

  • gRNAs

The following prpf8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the prpf8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa52155 XM_004911666.4
Latest version!
Xenopus tropicalis pre-mRNA processing factor 8 (prpf8), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa52155 XM_002936059.3
Latest version!
Xenopus tropicalis pre-mRNA processing factor 8 (prpf8), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa05611 XM_002936059.2
Latest version!
Xenopus tropicalis pre-mRNA processing factor 8 (prpf8), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OXa05611 XM_004911666.3
Latest version!
Xenopus tropicalis pre-mRNA processing factor 8 (prpf8), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa52155
    Clone ID Related Accession (Same CDS sequence) XM_002936059.3 , XM_004911666.4
    Accession Version XM_002936059.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7008bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pre-mRNA-processing-splicing factor 8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002936059.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCCGGGG TGTTTCCATA TAGACCACAG GCACCGCCAA TGCCCGGCCC CCCAGGTGGA 
    ACACCGGACT ACATGTCTGA AGAGAAGCTG CAGGAAAAAG CACGAAAATG GCAGCAGCTA
    CAGGCAAAAC GCTATTCAGA GAAAAGAAAA TTTGGGTTTG TGGATGCACA GAAAGAAGAT
    ATGCCTCCAG AACATGTTCG CAAAATTATT CGTGATCATG GAGACATGAC CAACAGAAAG
    TTCCGACATG ATAAGAGAGT TTACCTTGGA GCATTGAAGT ATATGCCACA TGCTGTCTTA
    AAGTTACTTG AAAACATGCC CATGCCTTGG GAGCAGATCC GTGATGTCCC TGTGCTGTAT
    CATATAACAG GAGCCATCTC ATTTGTTAAT GAGATTCCAT GGGTCATTGA GCCTGTCTAC
    ATAGCACAGT GGGGGTCAAT GTGGATTATG ATGAGGCGAG AAAAAAGAGA CAGACGGCAC
    TTCAAGAGGA TGAGGTTTCC TCCATTTGAT GATGAGGAGC CTCCACTGGA TTATGCTGAC
    AATATCCTTG ATGTTGAACC CCTGGAAGCC ATTCAGCTTG AACTGGACCC AGAGGAAGAT
    GCTCCAGTCA CTGACTGGTT CTATGATCAC CAGCCCTTAA GAGACAACAG AAAGTATGTG
    AATGGTACTA CTTATCAGCG GTGGCAGTTT ACACTTCCGA TGATGTCTAC ATTATATCGA
    TTGGCTAATC AGCTTCTGAC TGACCTGGTT GATGATAACT ACTTCTACCT GTTTGACCTA
    AAGGCATTCT TTACCTCCAA AGCATTAAAC ATGGCTATTC CTGGAGGGCC AAAATTTGAG
    CCTCTTGTTC GTGACATTAA CCTGCAGGAT GAAGATTGGA ATGAGTTTAA TGATATTAAC
    AAGATAATTA TCCGACAGCC AATTCGAACA GAATACAAGA TTGCTTTTCC TTACCTGTAC
    AACAATCTTC CTCACCATGT ACATTTAACC TGGTATCACA CACCAAATGT GGTTTTTATC
    AAAACTGAGG ATCCTGATTT ACCAGCATTC TATTTTGACC CACTGATTAA TCCCATATCA
    CACAGGCATT CTGTAAAGAG CCAAGAGCCA TTGCCAGATG ATGATGAGGA ATTTGAATTG
    CCAGAATATG TTGAACCATT CCTTAAAGAC ACTCCTCTGT ATTCAGATAA CACCGCTAAT
    GGAATTGCTT TACTTTGGGC TCCTCGTCCT TTCAACTTGC GTTCTGGTAG GACCAGGAGG
    GCTATTGATA TTCCTCTTGT GAAAAACTGG TACAGAGAGC ATTGTCCTGC TGGCCAGCCA
    GTAAAAGTCA GAGTATCTTA CCAGAAATTG CTGAAGTACT ATGTGCTAAA TGCTTTGAAA
    CACAGGCCTC CAAAAGCTCA GAAGAAAAGG TATCTCTTCC GTTCATTTAA AGCCACAAAA
    TTCTTTCAGT CGACAAAGTT GGACTGGGTG GAAGTAGGCC TGCAGGTTTG CCGCCAAGGC
    TATAACATGC TGAACTTGCT GATTCACAGA AAGAACCTTA ACTATCTTCA TCTGGATTAC
    AATTTCAATT TGAAGCCAGT AAAAACGCTG ACCACAAAGG AACGCAAAAA GTCTCGGTTC
    GGCAATGCCT TCCATCTTTG CCGCGAGGTC CTGCGTCTAA CCAAACTAGT TGTGGACAGC
    CATGTGCAAT ACAGACTGGG GAATGTTGAT GCTTTCCAGT TGGCAGACGG ATTACAGTAT
    ATTTTTGCCC ATGTGGGGCA GCTGACTGGA ATGTACCGTT ACAAGTACAA ACTAATGAGA
    CAAATTCGCA TGTGCAAGGA CCTGAAGCAT CTGATCTACT ACAGGTTCAA TACAGGTCCA
    GTTGGAAAGG GGCCTGGCTG TGGATTTTGG GCAGCAGGCT GGAGAGTCTG GCTCTTTTTT
    ATGCGTGGTA TCACACCTTT GCTGGAAAGG TGGCTTGGAA ATCTGCTTGC TAGACAATTT
    GAAGGTCGCC ATTCCAAAGG AGTTGCGAAA ACTGTGACAA AACAGCGAGT GGAGTCTCAC
    TTTGACTTGG AGCTCAGAGC TGCTGTTATG CATGACATAT TGGACATGAT GCCAGAGGGA
    ATCAAGCAGA ACAAAGCCAG AACCATCTTG CAGCACTTGA GTGAAGCTTG GAGATGCTGG
    AAAGCTAACA TTCCCTGGAA GGTACCAGGT CTTCCAACAC CCATTGAAAA CATGATTCTG
    CGATATGTAA AGGCCAAAGC GGACTGGTGG ACAAATACTG CGCATTACAA CAGAGAACGT
    ATTAGACGTG GTGCCACTGT CGACAAAACT GTGTGTAAGA AGAATCTGGG CCGTCTGACT
    CGTTTGTACT TGAAAGCAGA ACAAGAGAGA CAGCACAACT ACCTGAAGGA TGGGCCTTAC
    ATTACTGCAG AGGAGGCAGT TGCTGTGTAC ACTACCACTG TCCACTGGCT AGAGAGTAGG
    AGGTTTTCTC CAATTCCTTT TCCTCCTCTT TCCTACAAAC ATGATACAAA ACTACTCATT
    CTAGCACTTG AGAGACTGAA AGAAGCATAC AGTGTGAAGT CGCGTTTGAA TCAGTCTCAG
    AGGGAAGAAT TGGGTTTAAT CGAACAGGCG TATGACAACC CTCACGAAGC TCTGTCCAGA
    ATCAAGCGTC ACTTGCTCAC CCAAAGAGCT TTCAAGGAGG TTGGAATAGA GTTTATGGAT
    CTCTACAGCC ACCTTGTTCC TGTTTATGAC GTGGAACCAC TGGAAAAAAT TACTGATGCC
    TACCTGGATC AGTACCTGTG GTATGAAGCA GATAAAAGAA GGCTTTTCCC TCCATGGATC
    AAGCCTGCAG ATACAGAGCC ACCTCCCTTG TTGGTCTACA AGTGGTGCCA AGGCATAAAT
    AACCTGCAGG ATGTTTGGGA CACCACAGAA GGTGAATGCA ATGTGATGCT GGAGTCACGC
    TTTGAAAAGA TGTATGAAAA GATTGACTTG ACTCTGCTGA ATCGTCTGCT GCGTCTAATT
    GTTGATCACA ATATTGCTGA TTACATGACT GCAAAAAACA ACGTTGTGAT AAATTACAAG
    GATATGAACC ACACAAATTC CTATGGCATT ATTCGTGGTC TCCAGTTTGC TTCGTTTATT
    GTCCAGTACT ATGGTCTGGT GATGGATCTT CTGGTTCTTG GTTTACACAG AGCTAGTGAA
    ATGGCTGGGC CACCACAAAT GCCAAATGAC TTTCTAAGTT TCCAAGATGT GGCAACAGAA
    TCGGCCCATC CTATCCGCCT GTTTTGTCGA TACATTGACC GCATTCACAT TTTTTTCAGA
    TTTTCAGCTG ATGAAGCCAG AGACCTTATT CAGAGATATT TAACAGAACA TCCAGATCCC
    AATAATGAGA ATATTGTGGG CTACAACAAT AAAAAGTGTT GGCCAAGGGA TGCCAGAATG
    CGCCTCATGA AACATGATGT AAATCTGGGC CGTGCAGTGT TTTGGGATAT AAAAAATAGA
    CTGCCTCGTT CTGTCACCAC TGTGCAGTGG GAGAACAGTT TTGTGTCTGT GTACAGTAAA
    GACAATCCAA ACCTGCTGTT TAATATGTGC GGCTTTGAAT GCCGCATACT TCCCAAATGC
    CGCACAAGCT ATGAAGAGTT TACGCACAAG GATGGTGTCT GGAACCTGCA AAATGAAGTG
    ACCAAAGAAC GCACAGCTCA GTGCTTCTTA AGAGTTGATG ACGAGTCCAT GCAGAGATTT
    CACAACAGAG TCCGTCAAAT TCTTATGGCT TCCGGGTCCA CAACCTTTAC AAAAATTGTG
    AACAAGTGGA ATACAGCACT TATTGGCCTA ATGACTTATT TCCGTGAGGC TGTGGTCAAC
    ACCCAAGAAC TACTTGATCT GCTGGTGAAA TGTGAGAACA AAATCCAAAC AAGAATAAAG
    ATTGGGCTGA ACTCTAAAAT GCCAAGCAGG TTCCCGCCTG TTGTTTTTTA CACACCAAAG
    GAACTTGGAG GTTTGGGCAT GTTGTCCATG GGACATGTTT TAATTCCACA GTCTGACCTA
    AGGTGGTCTA AGCAGACAGA TGTTGGCATA ACCCATTTCC GTTCTGGCAT GAGTCACGAG
    GAGGATCAAC TGATTCCTAA TCTGTATCGT TACATTCAGC CTTGGGAAAG TGAGTTTATA
    GACTCTCAGA GAGTCTGGGC TGAGTATGCC TTAAAGAGGC AGGAGGCGAT TGCTCAAAAC
    AGACGCCTGA CGCTTGAAGA CTTAGAAGAC TCATGGGACA GGGGAATTCC ACGTATTAAT
    ACACTGTTTC AGAAAGATCG GCACACACTT GCTTATGACA AGGGTTGGAG AGTTCGCACA
    GACTTTAAAC AGTACCAGGT GCTGAAACAA AACCCGTTCT GGTGGACACA TCAGCGTCAT
    GATGGAAAGC TATGGAATCT GAACAACTAC CGCACAGATA TGATTCAGGC CCTTGGAGGG
    GTGGAGGGTA TACTGGAGCA CACGCTATTT AAAGGCACTT ACTTTCCCAC ATGGGAGGGT
    CTTTTCTGGG AAAAGGCCAG CGGCTTTGAA GAATCAATGA AATGGAAGAA ACTGACCAAT
    GCTCAGCGTT CTGGTTTAAA CCAGATACCA AACAGAAGAT TTACACTTTG GTGGTCTCCC
    ACAATCAACA GAGCCAACGT CTATGTTGGA TTCCAGGTGC AACTGGATTT GACTGGGATT
    TTCATGCATG GCAAGATTCC TACATTAAAA ATCTCATTGA TTCAGATATT CAGGGCCCAC
    TTGTGGCAAA AGATTCATGA AAGCATTGTC ATGGATTTAT GTCAGGTGTT TGATCAGGAG
    CTGGATGCTT TGGAGATTGA GACTGTGCAG AAAGAGACCA TCCACCCTAG GAAGTCCTAT
    AAGATGAACT CGTCCTGTGC TGATATTCTG CTCTTTGCCT CCTACAAATG GAATGTGTCT
    CGTCCCTCCT TGCTTGCTGA CTCAAAGGAT GTAATGGACA GCACAACCAC CCAGAAGTAC
    TGGATTGATA TACAGCTGAG ATGGGGTGAC TATGACTCCC ACGACATTGA AAGATATGCA
    CGTGCCAAAT TTTTGGATTA TACAACAGAT AACATGAGTA TCTATCCCTC ACCTACTGGT
    GTTCTTATTG CCATTGACCT TGCCTATAAC CTGCACAGTG CATATGGAAA CTGGTTCCCA
    GGGGGAAAGC CTCTAATACA GCAAGCAATG GCAAAGATAA TGAAGGCAAA CCCTGCCCTC
    TACGTGCTGC GGGAGCGCAT CCGTAAAGGA TTACAGCTTT ACTCTTCTGA GCCCACTGAG
    CCTTACCTGT CTTCTCAGAA CTATGGGGAA CTCTTCTCCA ATCAAATCAT CTGGTTTGTG
    GATGACACAA ATGTATACAG AGTCACCATC CACAAGACCT TTGAAGGTAA CCTGACAACC
    AAGCCAATCA ATGGAGCCAT TTTCATCTTT AATCCCAGGA CTGGCCAGCT CTTCTTAAAA
    ATTATTCATA CTTCTGTGTG GGCTGGACAG AAGAGATTGG GTCAGCTGGC CAAATGGAAA
    ACTGCAGAAG AAGTAGCTGC ATTGATTCGA TCTCTTCCAG TTGAGGAGCA GCCGAAACAA
    ATCATTGTCA CCAGGAAAGG CATGTTGGAT CCACTCGAGG TGCACTTGCT TGACTTCCCA
    AACATTGTCA TCAAAGGATC AGAATTACAG TTGCCTTTCC AGGCCTGTCT GAAGGTGGAG
    AAATTTGGTG ATCTGATCTT AAAGGCTACA GAGCCTCAGA TGGTTCTGTT CAATCTATAT
    GATGACTGGC TGAAGACGAT CTCTTCATAC ACGGCATTCT CTCGATTAAT TTTAATTCTA
    AGAGCCTTAC ATGTCAACAA TGACCGTGCA AAAGTGATCT TGAAACCTGA CAAGACCACT
    GTCACAGAGC CCCATCATAT CTGGCCCACA CTGACAGATG AGGAATGGAT TAAGGTTGAA
    GTTCAGCTAA AGGATTTGAT TTTAGCAGAT TATGGAAAGA AGAACAACGT AAATGTGGCC
    TCACTGACAC AGTCTGAAAT CAGAGACATC ATTCTGGGTA TGGAAATTTC TGCTCCATCC
    CAGCAAAGGC AGCAGATTGC AGAAATTGAA AAGCAAACAA AAGAACAGTC TCAGCTCACT
    GCTACTCAGA CGCGCACTGT CAACAAACAC GGAGATGAGA TTATCACATC TACCACCAGC
    AACTACGAAA CACAGACGTT CTCTTCAAAG ACTGAGTGGC GAGTCAGAGC AATATCTGCT
    GCCAACCTTC ACCTCCGAAC CAATCACATC TATGTATCGT CTGATGACAT TAAGGAAACT
    GGATACACCT ACATATTGCC CAAAAATGTG CTTAAGAAAT TCATTTGCAT CTCTGATCTT
    AGAGCACAGA TTGCTGGTTA CCTGTATGGT GTGAGCCCCC CAGATAATCC CCAAGTGAAG
    GAAATCCGTT GCATTGTTAT GGTGCCCCAG TGGGGAACTC ATCAAACTGT CCACCTGCCA
    AGCCAGCTGC CCCAACATGA ATACCTTAAG GAAATGGAGC CATTAGGATG GGTTCACACT
    CAGCCAAATG AGTCCCCACA GTTGTCACCT CAAGATGTCA CCACCCATGC CAAAGTAATG
    GCTGACAATC CTGCCTGGGA TGGAGAGAAA ACCATAATCA TTACTTGCAG CTTTACTCCA
    GGTTCATGCA CGTTAACCGC TTATAAACTG ACTCCCAGTG GTTATGAATG GGGACGGCAA
    AACACAGATA AAGGAAACAA TCCCAAGGGC TACTTGCCAT CGCATTATGA GAGGGTCCAG
    ATGCTGCTGT CTGATCGCTT CCTTGGGTTC TTCATGGTGC CAGCACAAGC GTCCTGGAAC
    TACAACTTCA TGGGTGTGAG ACATGACCCA AACATGAAAT ATGAGTTACA GCTGGCCAAC
    CCGAAGGAGT TTTACCATGA AGTACACCGG CCATCGCATT TCCTAAACTT TGCTCTGCTG
    CAGGAGGGTG AGGTGTATTC GGCTGACCGG GAAGATCTGT ATGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002936105.2
    CDS243..7250
    Translation

    Target ORF information:

    RefSeq Version XM_002936059.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pre-mRNA processing factor 8 (prpf8), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002936059.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCCGGGG TGTTTCCATA TAGACCACAG GCACCGCCAA TGCCCGGCCC CCCAGGTGGA 
    ACACCGGACT ACATGTCTGA AGAGAAGCTG CAGGAAAAAG CACGAAAATG GCAGCAGCTA
    CAGGCAAAAC GCTATTCAGA GAAAAGAAAA TTTGGGTTTG TGGATGCACA GAAAGAAGAT
    ATGCCTCCAG AACATGTTCG CAAAATTATT CGTGATCATG GAGACATGAC CAACAGAAAG
    TTCCGACATG ATAAGAGAGT TTACCTTGGA GCATTGAAGT ATATGCCACA TGCTGTCTTA
    AAGTTACTTG AAAACATGCC CATGCCTTGG GAGCAGATCC GTGATGTCCC TGTGCTGTAT
    CATATAACAG GAGCCATCTC ATTTGTTAAT GAGATTCCAT GGGTCATTGA GCCTGTCTAC
    ATAGCACAGT GGGGGTCAAT GTGGATTATG ATGAGGCGAG AAAAAAGAGA CAGACGGCAC
    TTCAAGAGGA TGAGGTTTCC TCCATTTGAT GATGAGGAGC CTCCACTGGA TTATGCTGAC
    AATATCCTTG ATGTTGAACC CCTGGAAGCC ATTCAGCTTG AACTGGACCC AGAGGAAGAT
    GCTCCAGTCA CTGACTGGTT CTATGATCAC CAGCCCTTAA GAGACAACAG AAAGTATGTG
    AATGGTACTA CTTATCAGCG GTGGCAGTTT ACACTTCCGA TGATGTCTAC ATTATATCGA
    TTGGCTAATC AGCTTCTGAC TGACCTGGTT GATGATAACT ACTTCTACCT GTTTGACCTA
    AAGGCATTCT TTACCTCCAA AGCATTAAAC ATGGCTATTC CTGGAGGGCC AAAATTTGAG
    CCTCTTGTTC GTGACATTAA CCTGCAGGAT GAAGATTGGA ATGAGTTTAA TGATATTAAC
    AAGATAATTA TCCGACAGCC AATTCGAACA GAATACAAGA TTGCTTTTCC TTACCTGTAC
    AACAATCTTC CTCACCATGT ACATTTAACC TGGTATCACA CACCAAATGT GGTTTTTATC
    AAAACTGAGG ATCCTGATTT ACCAGCATTC TATTTTGACC CACTGATTAA TCCCATATCA
    CACAGGCATT CTGTAAAGAG CCAAGAGCCA TTGCCAGATG ATGATGAGGA ATTTGAATTG
    CCAGAATATG TTGAACCATT CCTTAAAGAC ACTCCTCTGT ATTCAGATAA CACCGCTAAT
    GGAATTGCTT TACTTTGGGC TCCTCGTCCT TTCAACTTGC GTTCTGGTAG GACCAGGAGG
    GCTATTGATA TTCCTCTTGT GAAAAACTGG TACAGAGAGC ATTGTCCTGC TGGCCAGCCA
    GTAAAAGTCA GAGTATCTTA CCAGAAATTG CTGAAGTACT ATGTGCTAAA TGCTTTGAAA
    CACAGGCCTC CAAAAGCTCA GAAGAAAAGG TATCTCTTCC GTTCATTTAA AGCCACAAAA
    TTCTTTCAGT CGACAAAGTT GGACTGGGTG GAAGTAGGCC TGCAGGTTTG CCGCCAAGGC
    TATAACATGC TGAACTTGCT GATTCACAGA AAGAACCTTA ACTATCTTCA TCTGGATTAC
    AATTTCAATT TGAAGCCAGT AAAAACGCTG ACCACAAAGG AACGCAAAAA GTCTCGGTTC
    GGCAATGCCT TCCATCTTTG CCGCGAGGTC CTGCGTCTAA CCAAACTAGT TGTGGACAGC
    CATGTGCAAT ACAGACTGGG GAATGTTGAT GCTTTCCAGT TGGCAGACGG ATTACAGTAT
    ATTTTTGCCC ATGTGGGGCA GCTGACTGGA ATGTACCGTT ACAAGTACAA ACTAATGAGA
    CAAATTCGCA TGTGCAAGGA CCTGAAGCAT CTGATCTACT ACAGGTTCAA TACAGGTCCA
    GTTGGAAAGG GGCCTGGCTG TGGATTTTGG GCAGCAGGCT GGAGAGTCTG GCTCTTTTTT
    ATGCGTGGTA TCACACCTTT GCTGGAAAGG TGGCTTGGAA ATCTGCTTGC TAGACAATTT
    GAAGGTCGCC ATTCCAAAGG AGTTGCGAAA ACTGTGACAA AACAGCGAGT GGAGTCTCAC
    TTTGACTTGG AGCTCAGAGC TGCTGTTATG CATGACATAT TGGACATGAT GCCAGAGGGA
    ATCAAGCAGA ACAAAGCCAG AACCATCTTG CAGCACTTGA GTGAAGCTTG GAGATGCTGG
    AAAGCTAACA TTCCCTGGAA GGTACCAGGT CTTCCAACAC CCATTGAAAA CATGATTCTG
    CGATATGTAA AGGCCAAAGC GGACTGGTGG ACAAATACTG CGCATTACAA CAGAGAACGT
    ATTAGACGTG GTGCCACTGT CGACAAAACT GTGTGTAAGA AGAATCTGGG CCGTCTGACT
    CGTTTGTACT TGAAAGCAGA ACAAGAGAGA CAGCACAACT ACCTGAAGGA TGGGCCTTAC
    ATTACTGCAG AGGAGGCAGT TGCTGTGTAC ACTACCACTG TCCACTGGCT AGAGAGTAGG
    AGGTTTTCTC CAATTCCTTT TCCTCCTCTT TCCTACAAAC ATGATACAAA ACTACTCATT
    CTAGCACTTG AGAGACTGAA AGAAGCATAC AGTGTGAAGT CGCGTTTGAA TCAGTCTCAG
    AGGGAAGAAT TGGGTTTAAT CGAACAGGCG TATGACAACC CTCACGAAGC TCTGTCCAGA
    ATCAAGCGTC ACTTGCTCAC CCAAAGAGCT TTCAAGGAGG TTGGAATAGA GTTTATGGAT
    CTCTACAGCC ACCTTGTTCC TGTTTATGAC GTGGAACCAC TGGAAAAAAT TACTGATGCC
    TACCTGGATC AGTACCTGTG GTATGAAGCA GATAAAAGAA GGCTTTTCCC TCCATGGATC
    AAGCCTGCAG ATACAGAGCC ACCTCCCTTG TTGGTCTACA AGTGGTGCCA AGGCATAAAT
    AACCTGCAGG ATGTTTGGGA CACCACAGAA GGTGAATGCA ATGTGATGCT GGAGTCACGC
    TTTGAAAAGA TGTATGAAAA GATTGACTTG ACTCTGCTGA ATCGTCTGCT GCGTCTAATT
    GTTGATCACA ATATTGCTGA TTACATGACT GCAAAAAACA ACGTTGTGAT AAATTACAAG
    GATATGAACC ACACAAATTC CTATGGCATT ATTCGTGGTC TCCAGTTTGC TTCGTTTATT
    GTCCAGTACT ATGGTCTGGT GATGGATCTT CTGGTTCTTG GTTTACACAG AGCTAGTGAA
    ATGGCTGGGC CACCACAAAT GCCAAATGAC TTTCTAAGTT TCCAAGATGT GGCAACAGAA
    TCGGCCCATC CTATCCGCCT GTTTTGTCGA TACATTGACC GCATTCACAT TTTTTTCAGA
    TTTTCAGCTG ATGAAGCCAG AGACCTTATT CAGAGATATT TAACAGAACA TCCAGATCCC
    AATAATGAGA ATATTGTGGG CTACAACAAT AAAAAGTGTT GGCCAAGGGA TGCCAGAATG
    CGCCTCATGA AACATGATGT AAATCTGGGC CGTGCAGTGT TTTGGGATAT AAAAAATAGA
    CTGCCTCGTT CTGTCACCAC TGTGCAGTGG GAGAACAGTT TTGTGTCTGT GTACAGTAAA
    GACAATCCAA ACCTGCTGTT TAATATGTGC GGCTTTGAAT GCCGCATACT TCCCAAATGC
    CGCACAAGCT ATGAAGAGTT TACGCACAAG GATGGTGTCT GGAACCTGCA AAATGAAGTG
    ACCAAAGAAC GCACAGCTCA GTGCTTCTTA AGAGTTGATG ACGAGTCCAT GCAGAGATTT
    CACAACAGAG TCCGTCAAAT TCTTATGGCT TCCGGGTCCA CAACCTTTAC AAAAATTGTG
    AACAAGTGGA ATACAGCACT TATTGGCCTA ATGACTTATT TCCGTGAGGC TGTGGTCAAC
    ACCCAAGAAC TACTTGATCT GCTGGTGAAA TGTGAGAACA AAATCCAAAC AAGAATAAAG
    ATTGGGCTGA ACTCTAAAAT GCCAAGCAGG TTCCCGCCTG TTGTTTTTTA CACACCAAAG
    GAACTTGGAG GTTTGGGCAT GTTGTCCATG GGACATGTTT TAATTCCACA GTCTGACCTA
    AGGTGGTCTA AGCAGACAGA TGTTGGCATA ACCCATTTCC GTTCTGGCAT GAGTCACGAG
    GAGGATCAAC TGATTCCTAA TCTGTATCGT TACATTCAGC CTTGGGAAAG TGAGTTTATA
    GACTCTCAGA GAGTCTGGGC TGAGTATGCC TTAAAGAGGC AGGAGGCGAT TGCTCAAAAC
    AGACGCCTGA CGCTTGAAGA CTTAGAAGAC TCATGGGACA GGGGAATTCC ACGTATTAAT
    ACACTGTTTC AGAAAGATCG GCACACACTT GCTTATGACA AGGGTTGGAG AGTTCGCACA
    GACTTTAAAC AGTACCAGGT GCTGAAACAA AACCCGTTCT GGTGGACACA TCAGCGTCAT
    GATGGAAAGC TATGGAATCT GAACAACTAC CGCACAGATA TGATTCAGGC CCTTGGAGGG
    GTGGAGGGTA TACTGGAGCA CACGCTATTT AAAGGCACTT ACTTTCCCAC ATGGGAGGGT
    CTTTTCTGGG AAAAGGCCAG CGGCTTTGAA GAATCAATGA AATGGAAGAA ACTGACCAAT
    GCTCAGCGTT CTGGTTTAAA CCAGATACCA AACAGAAGAT TTACACTTTG GTGGTCTCCC
    ACAATCAACA GAGCCAACGT CTATGTTGGA TTCCAGGTGC AACTGGATTT GACTGGGATT
    TTCATGCATG GCAAGATTCC TACATTAAAA ATCTCATTGA TTCAGATATT CAGGGCCCAC
    TTGTGGCAAA AGATTCATGA AAGCATTGTC ATGGATTTAT GTCAGGTGTT TGATCAGGAG
    CTGGATGCTT TGGAGATTGA GACTGTGCAG AAAGAGACCA TCCACCCTAG GAAGTCCTAT
    AAGATGAACT CGTCCTGTGC TGATATTCTG CTCTTTGCCT CCTACAAATG GAATGTGTCT
    CGTCCCTCCT TGCTTGCTGA CTCAAAGGAT GTAATGGACA GCACAACCAC CCAGAAGTAC
    TGGATTGATA TACAGCTGAG ATGGGGTGAC TATGACTCCC ACGACATTGA AAGATATGCA
    CGTGCCAAAT TTTTGGATTA TACAACAGAT AACATGAGTA TCTATCCCTC ACCTACTGGT
    GTTCTTATTG CCATTGACCT TGCCTATAAC CTGCACAGTG CATATGGAAA CTGGTTCCCA
    GGGGGAAAGC CTCTAATACA GCAAGCAATG GCAAAGATAA TGAAGGCAAA CCCTGCCCTC
    TACGTGCTGC GGGAGCGCAT CCGTAAAGGA TTACAGCTTT ACTCTTCTGA GCCCACTGAG
    CCTTACCTGT CTTCTCAGAA CTATGGGGAA CTCTTCTCCA ATCAAATCAT CTGGTTTGTG
    GATGACACAA ATGTATACAG AGTCACCATC CACAAGACCT TTGAAGGTAA CCTGACAACC
    AAGCCAATCA ATGGAGCCAT TTTCATCTTT AATCCCAGGA CTGGCCAGCT CTTCTTAAAA
    ATTATTCATA CTTCTGTGTG GGCTGGACAG AAGAGATTGG GTCAGCTGGC CAAATGGAAA
    ACTGCAGAAG AAGTAGCTGC ATTGATTCGA TCTCTTCCAG TTGAGGAGCA GCCGAAACAA
    ATCATTGTCA CCAGGAAAGG CATGTTGGAT CCACTCGAGG TGCACTTGCT TGACTTCCCA
    AACATTGTCA TCAAAGGATC AGAATTACAG TTGCCTTTCC AGGCCTGTCT GAAGGTGGAG
    AAATTTGGTG ATCTGATCTT AAAGGCTACA GAGCCTCAGA TGGTTCTGTT CAATCTATAT
    GATGACTGGC TGAAGACGAT CTCTTCATAC ACGGCATTCT CTCGATTAAT TTTAATTCTA
    AGAGCCTTAC ATGTCAACAA TGACCGTGCA AAAGTGATCT TGAAACCTGA CAAGACCACT
    GTCACAGAGC CCCATCATAT CTGGCCCACA CTGACAGATG AGGAATGGAT TAAGGTTGAA
    GTTCAGCTAA AGGATTTGAT TTTAGCAGAT TATGGAAAGA AGAACAACGT AAATGTGGCC
    TCACTGACAC AGTCTGAAAT CAGAGACATC ATTCTGGGTA TGGAAATTTC TGCTCCATCC
    CAGCAAAGGC AGCAGATTGC AGAAATTGAA AAGCAAACAA AAGAACAGTC TCAGCTCACT
    GCTACTCAGA CGCGCACTGT CAACAAACAC GGAGATGAGA TTATCACATC TACCACCAGC
    AACTACGAAA CACAGACGTT CTCTTCAAAG ACTGAGTGGC GAGTCAGAGC AATATCTGCT
    GCCAACCTTC ACCTCCGAAC CAATCACATC TATGTATCGT CTGATGACAT TAAGGAAACT
    GGATACACCT ACATATTGCC CAAAAATGTG CTTAAGAAAT TCATTTGCAT CTCTGATCTT
    AGAGCACAGA TTGCTGGTTA CCTGTATGGT GTGAGCCCCC CAGATAATCC CCAAGTGAAG
    GAAATCCGTT GCATTGTTAT GGTGCCCCAG TGGGGAACTC ATCAAACTGT CCACCTGCCA
    AGCCAGCTGC CCCAACATGA ATACCTTAAG GAAATGGAGC CATTAGGATG GGTTCACACT
    CAGCCAAATG AGTCCCCACA GTTGTCACCT CAAGATGTCA CCACCCATGC CAAAGTAATG
    GCTGACAATC CTGCCTGGGA TGGAGAGAAA ACCATAATCA TTACTTGCAG CTTTACTCCA
    GGTTCATGCA CGTTAACCGC TTATAAACTG ACTCCCAGTG GTTATGAATG GGGACGGCAA
    AACACAGATA AAGGAAACAA TCCCAAGGGC TACTTGCCAT CGCATTATGA GAGGGTCCAG
    ATGCTGCTGT CTGATCGCTT CCTTGGGTTC TTCATGGTGC CAGCACAAGC GTCCTGGAAC
    TACAACTTCA TGGGTGTGAG ACATGACCCA AACATGAAAT ATGAGTTACA GCTGGCCAAC
    CCGAAGGAGT TTTACCATGA AGTACACCGG CCATCGCATT TCCTAAACTT TGCTCTGCTG
    CAGGAGGGTG AGGTGTATTC GGCTGACCGG GAAGATCTGT ATGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52155
    Clone ID Related Accession (Same CDS sequence) XM_002936059.3 , XM_004911666.4
    Accession Version XM_004911666.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7008bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pre-mRNA-processing-splicing factor 8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004911666.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCCGGGG TGTTTCCATA TAGACCACAG GCACCGCCAA TGCCCGGCCC CCCAGGTGGA 
    ACACCGGACT ACATGTCTGA AGAGAAGCTG CAGGAAAAAG CACGAAAATG GCAGCAGCTA
    CAGGCAAAAC GCTATTCAGA GAAAAGAAAA TTTGGGTTTG TGGATGCACA GAAAGAAGAT
    ATGCCTCCAG AACATGTTCG CAAAATTATT CGTGATCATG GAGACATGAC CAACAGAAAG
    TTCCGACATG ATAAGAGAGT TTACCTTGGA GCATTGAAGT ATATGCCACA TGCTGTCTTA
    AAGTTACTTG AAAACATGCC CATGCCTTGG GAGCAGATCC GTGATGTCCC TGTGCTGTAT
    CATATAACAG GAGCCATCTC ATTTGTTAAT GAGATTCCAT GGGTCATTGA GCCTGTCTAC
    ATAGCACAGT GGGGGTCAAT GTGGATTATG ATGAGGCGAG AAAAAAGAGA CAGACGGCAC
    TTCAAGAGGA TGAGGTTTCC TCCATTTGAT GATGAGGAGC CTCCACTGGA TTATGCTGAC
    AATATCCTTG ATGTTGAACC CCTGGAAGCC ATTCAGCTTG AACTGGACCC AGAGGAAGAT
    GCTCCAGTCA CTGACTGGTT CTATGATCAC CAGCCCTTAA GAGACAACAG AAAGTATGTG
    AATGGTACTA CTTATCAGCG GTGGCAGTTT ACACTTCCGA TGATGTCTAC ATTATATCGA
    TTGGCTAATC AGCTTCTGAC TGACCTGGTT GATGATAACT ACTTCTACCT GTTTGACCTA
    AAGGCATTCT TTACCTCCAA AGCATTAAAC ATGGCTATTC CTGGAGGGCC AAAATTTGAG
    CCTCTTGTTC GTGACATTAA CCTGCAGGAT GAAGATTGGA ATGAGTTTAA TGATATTAAC
    AAGATAATTA TCCGACAGCC AATTCGAACA GAATACAAGA TTGCTTTTCC TTACCTGTAC
    AACAATCTTC CTCACCATGT ACATTTAACC TGGTATCACA CACCAAATGT GGTTTTTATC
    AAAACTGAGG ATCCTGATTT ACCAGCATTC TATTTTGACC CACTGATTAA TCCCATATCA
    CACAGGCATT CTGTAAAGAG CCAAGAGCCA TTGCCAGATG ATGATGAGGA ATTTGAATTG
    CCAGAATATG TTGAACCATT CCTTAAAGAC ACTCCTCTGT ATTCAGATAA CACCGCTAAT
    GGAATTGCTT TACTTTGGGC TCCTCGTCCT TTCAACTTGC GTTCTGGTAG GACCAGGAGG
    GCTATTGATA TTCCTCTTGT GAAAAACTGG TACAGAGAGC ATTGTCCTGC TGGCCAGCCA
    GTAAAAGTCA GAGTATCTTA CCAGAAATTG CTGAAGTACT ATGTGCTAAA TGCTTTGAAA
    CACAGGCCTC CAAAAGCTCA GAAGAAAAGG TATCTCTTCC GTTCATTTAA AGCCACAAAA
    TTCTTTCAGT CGACAAAGTT GGACTGGGTG GAAGTAGGCC TGCAGGTTTG CCGCCAAGGC
    TATAACATGC TGAACTTGCT GATTCACAGA AAGAACCTTA ACTATCTTCA TCTGGATTAC
    AATTTCAATT TGAAGCCAGT AAAAACGCTG ACCACAAAGG AACGCAAAAA GTCTCGGTTC
    GGCAATGCCT TCCATCTTTG CCGCGAGGTC CTGCGTCTAA CCAAACTAGT TGTGGACAGC
    CATGTGCAAT ACAGACTGGG GAATGTTGAT GCTTTCCAGT TGGCAGACGG ATTACAGTAT
    ATTTTTGCCC ATGTGGGGCA GCTGACTGGA ATGTACCGTT ACAAGTACAA ACTAATGAGA
    CAAATTCGCA TGTGCAAGGA CCTGAAGCAT CTGATCTACT ACAGGTTCAA TACAGGTCCA
    GTTGGAAAGG GGCCTGGCTG TGGATTTTGG GCAGCAGGCT GGAGAGTCTG GCTCTTTTTT
    ATGCGTGGTA TCACACCTTT GCTGGAAAGG TGGCTTGGAA ATCTGCTTGC TAGACAATTT
    GAAGGTCGCC ATTCCAAAGG AGTTGCGAAA ACTGTGACAA AACAGCGAGT GGAGTCTCAC
    TTTGACTTGG AGCTCAGAGC TGCTGTTATG CATGACATAT TGGACATGAT GCCAGAGGGA
    ATCAAGCAGA ACAAAGCCAG AACCATCTTG CAGCACTTGA GTGAAGCTTG GAGATGCTGG
    AAAGCTAACA TTCCCTGGAA GGTACCAGGT CTTCCAACAC CCATTGAAAA CATGATTCTG
    CGATATGTAA AGGCCAAAGC GGACTGGTGG ACAAATACTG CGCATTACAA CAGAGAACGT
    ATTAGACGTG GTGCCACTGT CGACAAAACT GTGTGTAAGA AGAATCTGGG CCGTCTGACT
    CGTTTGTACT TGAAAGCAGA ACAAGAGAGA CAGCACAACT ACCTGAAGGA TGGGCCTTAC
    ATTACTGCAG AGGAGGCAGT TGCTGTGTAC ACTACCACTG TCCACTGGCT AGAGAGTAGG
    AGGTTTTCTC CAATTCCTTT TCCTCCTCTT TCCTACAAAC ATGATACAAA ACTACTCATT
    CTAGCACTTG AGAGACTGAA AGAAGCATAC AGTGTGAAGT CGCGTTTGAA TCAGTCTCAG
    AGGGAAGAAT TGGGTTTAAT CGAACAGGCG TATGACAACC CTCACGAAGC TCTGTCCAGA
    ATCAAGCGTC ACTTGCTCAC CCAAAGAGCT TTCAAGGAGG TTGGAATAGA GTTTATGGAT
    CTCTACAGCC ACCTTGTTCC TGTTTATGAC GTGGAACCAC TGGAAAAAAT TACTGATGCC
    TACCTGGATC AGTACCTGTG GTATGAAGCA GATAAAAGAA GGCTTTTCCC TCCATGGATC
    AAGCCTGCAG ATACAGAGCC ACCTCCCTTG TTGGTCTACA AGTGGTGCCA AGGCATAAAT
    AACCTGCAGG ATGTTTGGGA CACCACAGAA GGTGAATGCA ATGTGATGCT GGAGTCACGC
    TTTGAAAAGA TGTATGAAAA GATTGACTTG ACTCTGCTGA ATCGTCTGCT GCGTCTAATT
    GTTGATCACA ATATTGCTGA TTACATGACT GCAAAAAACA ACGTTGTGAT AAATTACAAG
    GATATGAACC ACACAAATTC CTATGGCATT ATTCGTGGTC TCCAGTTTGC TTCGTTTATT
    GTCCAGTACT ATGGTCTGGT GATGGATCTT CTGGTTCTTG GTTTACACAG AGCTAGTGAA
    ATGGCTGGGC CACCACAAAT GCCAAATGAC TTTCTAAGTT TCCAAGATGT GGCAACAGAA
    TCGGCCCATC CTATCCGCCT GTTTTGTCGA TACATTGACC GCATTCACAT TTTTTTCAGA
    TTTTCAGCTG ATGAAGCCAG AGACCTTATT CAGAGATATT TAACAGAACA TCCAGATCCC
    AATAATGAGA ATATTGTGGG CTACAACAAT AAAAAGTGTT GGCCAAGGGA TGCCAGAATG
    CGCCTCATGA AACATGATGT AAATCTGGGC CGTGCAGTGT TTTGGGATAT AAAAAATAGA
    CTGCCTCGTT CTGTCACCAC TGTGCAGTGG GAGAACAGTT TTGTGTCTGT GTACAGTAAA
    GACAATCCAA ACCTGCTGTT TAATATGTGC GGCTTTGAAT GCCGCATACT TCCCAAATGC
    CGCACAAGCT ATGAAGAGTT TACGCACAAG GATGGTGTCT GGAACCTGCA AAATGAAGTG
    ACCAAAGAAC GCACAGCTCA GTGCTTCTTA AGAGTTGATG ACGAGTCCAT GCAGAGATTT
    CACAACAGAG TCCGTCAAAT TCTTATGGCT TCCGGGTCCA CAACCTTTAC AAAAATTGTG
    AACAAGTGGA ATACAGCACT TATTGGCCTA ATGACTTATT TCCGTGAGGC TGTGGTCAAC
    ACCCAAGAAC TACTTGATCT GCTGGTGAAA TGTGAGAACA AAATCCAAAC AAGAATAAAG
    ATTGGGCTGA ACTCTAAAAT GCCAAGCAGG TTCCCGCCTG TTGTTTTTTA CACACCAAAG
    GAACTTGGAG GTTTGGGCAT GTTGTCCATG GGACATGTTT TAATTCCACA GTCTGACCTA
    AGGTGGTCTA AGCAGACAGA TGTTGGCATA ACCCATTTCC GTTCTGGCAT GAGTCACGAG
    GAGGATCAAC TGATTCCTAA TCTGTATCGT TACATTCAGC CTTGGGAAAG TGAGTTTATA
    GACTCTCAGA GAGTCTGGGC TGAGTATGCC TTAAAGAGGC AGGAGGCGAT TGCTCAAAAC
    AGACGCCTGA CGCTTGAAGA CTTAGAAGAC TCATGGGACA GGGGAATTCC ACGTATTAAT
    ACACTGTTTC AGAAAGATCG GCACACACTT GCTTATGACA AGGGTTGGAG AGTTCGCACA
    GACTTTAAAC AGTACCAGGT GCTGAAACAA AACCCGTTCT GGTGGACACA TCAGCGTCAT
    GATGGAAAGC TATGGAATCT GAACAACTAC CGCACAGATA TGATTCAGGC CCTTGGAGGG
    GTGGAGGGTA TACTGGAGCA CACGCTATTT AAAGGCACTT ACTTTCCCAC ATGGGAGGGT
    CTTTTCTGGG AAAAGGCCAG CGGCTTTGAA GAATCAATGA AATGGAAGAA ACTGACCAAT
    GCTCAGCGTT CTGGTTTAAA CCAGATACCA AACAGAAGAT TTACACTTTG GTGGTCTCCC
    ACAATCAACA GAGCCAACGT CTATGTTGGA TTCCAGGTGC AACTGGATTT GACTGGGATT
    TTCATGCATG GCAAGATTCC TACATTAAAA ATCTCATTGA TTCAGATATT CAGGGCCCAC
    TTGTGGCAAA AGATTCATGA AAGCATTGTC ATGGATTTAT GTCAGGTGTT TGATCAGGAG
    CTGGATGCTT TGGAGATTGA GACTGTGCAG AAAGAGACCA TCCACCCTAG GAAGTCCTAT
    AAGATGAACT CGTCCTGTGC TGATATTCTG CTCTTTGCCT CCTACAAATG GAATGTGTCT
    CGTCCCTCCT TGCTTGCTGA CTCAAAGGAT GTAATGGACA GCACAACCAC CCAGAAGTAC
    TGGATTGATA TACAGCTGAG ATGGGGTGAC TATGACTCCC ACGACATTGA AAGATATGCA
    CGTGCCAAAT TTTTGGATTA TACAACAGAT AACATGAGTA TCTATCCCTC ACCTACTGGT
    GTTCTTATTG CCATTGACCT TGCCTATAAC CTGCACAGTG CATATGGAAA CTGGTTCCCA
    GGGGGAAAGC CTCTAATACA GCAAGCAATG GCAAAGATAA TGAAGGCAAA CCCTGCCCTC
    TACGTGCTGC GGGAGCGCAT CCGTAAAGGA TTACAGCTTT ACTCTTCTGA GCCCACTGAG
    CCTTACCTGT CTTCTCAGAA CTATGGGGAA CTCTTCTCCA ATCAAATCAT CTGGTTTGTG
    GATGACACAA ATGTATACAG AGTCACCATC CACAAGACCT TTGAAGGTAA CCTGACAACC
    AAGCCAATCA ATGGAGCCAT TTTCATCTTT AATCCCAGGA CTGGCCAGCT CTTCTTAAAA
    ATTATTCATA CTTCTGTGTG GGCTGGACAG AAGAGATTGG GTCAGCTGGC CAAATGGAAA
    ACTGCAGAAG AAGTAGCTGC ATTGATTCGA TCTCTTCCAG TTGAGGAGCA GCCGAAACAA
    ATCATTGTCA CCAGGAAAGG CATGTTGGAT CCACTCGAGG TGCACTTGCT TGACTTCCCA
    AACATTGTCA TCAAAGGATC AGAATTACAG TTGCCTTTCC AGGCCTGTCT GAAGGTGGAG
    AAATTTGGTG ATCTGATCTT AAAGGCTACA GAGCCTCAGA TGGTTCTGTT CAATCTATAT
    GATGACTGGC TGAAGACGAT CTCTTCATAC ACGGCATTCT CTCGATTAAT TTTAATTCTA
    AGAGCCTTAC ATGTCAACAA TGACCGTGCA AAAGTGATCT TGAAACCTGA CAAGACCACT
    GTCACAGAGC CCCATCATAT CTGGCCCACA CTGACAGATG AGGAATGGAT TAAGGTTGAA
    GTTCAGCTAA AGGATTTGAT TTTAGCAGAT TATGGAAAGA AGAACAACGT AAATGTGGCC
    TCACTGACAC AGTCTGAAAT CAGAGACATC ATTCTGGGTA TGGAAATTTC TGCTCCATCC
    CAGCAAAGGC AGCAGATTGC AGAAATTGAA AAGCAAACAA AAGAACAGTC TCAGCTCACT
    GCTACTCAGA CGCGCACTGT CAACAAACAC GGAGATGAGA TTATCACATC TACCACCAGC
    AACTACGAAA CACAGACGTT CTCTTCAAAG ACTGAGTGGC GAGTCAGAGC AATATCTGCT
    GCCAACCTTC ACCTCCGAAC CAATCACATC TATGTATCGT CTGATGACAT TAAGGAAACT
    GGATACACCT ACATATTGCC CAAAAATGTG CTTAAGAAAT TCATTTGCAT CTCTGATCTT
    AGAGCACAGA TTGCTGGTTA CCTGTATGGT GTGAGCCCCC CAGATAATCC CCAAGTGAAG
    GAAATCCGTT GCATTGTTAT GGTGCCCCAG TGGGGAACTC ATCAAACTGT CCACCTGCCA
    AGCCAGCTGC CCCAACATGA ATACCTTAAG GAAATGGAGC CATTAGGATG GGTTCACACT
    CAGCCAAATG AGTCCCCACA GTTGTCACCT CAAGATGTCA CCACCCATGC CAAAGTAATG
    GCTGACAATC CTGCCTGGGA TGGAGAGAAA ACCATAATCA TTACTTGCAG CTTTACTCCA
    GGTTCATGCA CGTTAACCGC TTATAAACTG ACTCCCAGTG GTTATGAATG GGGACGGCAA
    AACACAGATA AAGGAAACAA TCCCAAGGGC TACTTGCCAT CGCATTATGA GAGGGTCCAG
    ATGCTGCTGT CTGATCGCTT CCTTGGGTTC TTCATGGTGC CAGCACAAGC GTCCTGGAAC
    TACAACTTCA TGGGTGTGAG ACATGACCCA AACATGAAAT ATGAGTTACA GCTGGCCAAC
    CCGAAGGAGT TTTACCATGA AGTACACCGG CCATCGCATT TCCTAAACTT TGCTCTGCTG
    CAGGAGGGTG AGGTGTATTC GGCTGACCGG GAAGATCTGT ATGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004911723.2
    CDS107..7114
    Translation

    Target ORF information:

    RefSeq Version XM_004911666.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pre-mRNA processing factor 8 (prpf8), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004911666.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCCGGGG TGTTTCCATA TAGACCACAG GCACCGCCAA TGCCCGGCCC CCCAGGTGGA 
    ACACCGGACT ACATGTCTGA AGAGAAGCTG CAGGAAAAAG CACGAAAATG GCAGCAGCTA
    CAGGCAAAAC GCTATTCAGA GAAAAGAAAA TTTGGGTTTG TGGATGCACA GAAAGAAGAT
    ATGCCTCCAG AACATGTTCG CAAAATTATT CGTGATCATG GAGACATGAC CAACAGAAAG
    TTCCGACATG ATAAGAGAGT TTACCTTGGA GCATTGAAGT ATATGCCACA TGCTGTCTTA
    AAGTTACTTG AAAACATGCC CATGCCTTGG GAGCAGATCC GTGATGTCCC TGTGCTGTAT
    CATATAACAG GAGCCATCTC ATTTGTTAAT GAGATTCCAT GGGTCATTGA GCCTGTCTAC
    ATAGCACAGT GGGGGTCAAT GTGGATTATG ATGAGGCGAG AAAAAAGAGA CAGACGGCAC
    TTCAAGAGGA TGAGGTTTCC TCCATTTGAT GATGAGGAGC CTCCACTGGA TTATGCTGAC
    AATATCCTTG ATGTTGAACC CCTGGAAGCC ATTCAGCTTG AACTGGACCC AGAGGAAGAT
    GCTCCAGTCA CTGACTGGTT CTATGATCAC CAGCCCTTAA GAGACAACAG AAAGTATGTG
    AATGGTACTA CTTATCAGCG GTGGCAGTTT ACACTTCCGA TGATGTCTAC ATTATATCGA
    TTGGCTAATC AGCTTCTGAC TGACCTGGTT GATGATAACT ACTTCTACCT GTTTGACCTA
    AAGGCATTCT TTACCTCCAA AGCATTAAAC ATGGCTATTC CTGGAGGGCC AAAATTTGAG
    CCTCTTGTTC GTGACATTAA CCTGCAGGAT GAAGATTGGA ATGAGTTTAA TGATATTAAC
    AAGATAATTA TCCGACAGCC AATTCGAACA GAATACAAGA TTGCTTTTCC TTACCTGTAC
    AACAATCTTC CTCACCATGT ACATTTAACC TGGTATCACA CACCAAATGT GGTTTTTATC
    AAAACTGAGG ATCCTGATTT ACCAGCATTC TATTTTGACC CACTGATTAA TCCCATATCA
    CACAGGCATT CTGTAAAGAG CCAAGAGCCA TTGCCAGATG ATGATGAGGA ATTTGAATTG
    CCAGAATATG TTGAACCATT CCTTAAAGAC ACTCCTCTGT ATTCAGATAA CACCGCTAAT
    GGAATTGCTT TACTTTGGGC TCCTCGTCCT TTCAACTTGC GTTCTGGTAG GACCAGGAGG
    GCTATTGATA TTCCTCTTGT GAAAAACTGG TACAGAGAGC ATTGTCCTGC TGGCCAGCCA
    GTAAAAGTCA GAGTATCTTA CCAGAAATTG CTGAAGTACT ATGTGCTAAA TGCTTTGAAA
    CACAGGCCTC CAAAAGCTCA GAAGAAAAGG TATCTCTTCC GTTCATTTAA AGCCACAAAA
    TTCTTTCAGT CGACAAAGTT GGACTGGGTG GAAGTAGGCC TGCAGGTTTG CCGCCAAGGC
    TATAACATGC TGAACTTGCT GATTCACAGA AAGAACCTTA ACTATCTTCA TCTGGATTAC
    AATTTCAATT TGAAGCCAGT AAAAACGCTG ACCACAAAGG AACGCAAAAA GTCTCGGTTC
    GGCAATGCCT TCCATCTTTG CCGCGAGGTC CTGCGTCTAA CCAAACTAGT TGTGGACAGC
    CATGTGCAAT ACAGACTGGG GAATGTTGAT GCTTTCCAGT TGGCAGACGG ATTACAGTAT
    ATTTTTGCCC ATGTGGGGCA GCTGACTGGA ATGTACCGTT ACAAGTACAA ACTAATGAGA
    CAAATTCGCA TGTGCAAGGA CCTGAAGCAT CTGATCTACT ACAGGTTCAA TACAGGTCCA
    GTTGGAAAGG GGCCTGGCTG TGGATTTTGG GCAGCAGGCT GGAGAGTCTG GCTCTTTTTT
    ATGCGTGGTA TCACACCTTT GCTGGAAAGG TGGCTTGGAA ATCTGCTTGC TAGACAATTT
    GAAGGTCGCC ATTCCAAAGG AGTTGCGAAA ACTGTGACAA AACAGCGAGT GGAGTCTCAC
    TTTGACTTGG AGCTCAGAGC TGCTGTTATG CATGACATAT TGGACATGAT GCCAGAGGGA
    ATCAAGCAGA ACAAAGCCAG AACCATCTTG CAGCACTTGA GTGAAGCTTG GAGATGCTGG
    AAAGCTAACA TTCCCTGGAA GGTACCAGGT CTTCCAACAC CCATTGAAAA CATGATTCTG
    CGATATGTAA AGGCCAAAGC GGACTGGTGG ACAAATACTG CGCATTACAA CAGAGAACGT
    ATTAGACGTG GTGCCACTGT CGACAAAACT GTGTGTAAGA AGAATCTGGG CCGTCTGACT
    CGTTTGTACT TGAAAGCAGA ACAAGAGAGA CAGCACAACT ACCTGAAGGA TGGGCCTTAC
    ATTACTGCAG AGGAGGCAGT TGCTGTGTAC ACTACCACTG TCCACTGGCT AGAGAGTAGG
    AGGTTTTCTC CAATTCCTTT TCCTCCTCTT TCCTACAAAC ATGATACAAA ACTACTCATT
    CTAGCACTTG AGAGACTGAA AGAAGCATAC AGTGTGAAGT CGCGTTTGAA TCAGTCTCAG
    AGGGAAGAAT TGGGTTTAAT CGAACAGGCG TATGACAACC CTCACGAAGC TCTGTCCAGA
    ATCAAGCGTC ACTTGCTCAC CCAAAGAGCT TTCAAGGAGG TTGGAATAGA GTTTATGGAT
    CTCTACAGCC ACCTTGTTCC TGTTTATGAC GTGGAACCAC TGGAAAAAAT TACTGATGCC
    TACCTGGATC AGTACCTGTG GTATGAAGCA GATAAAAGAA GGCTTTTCCC TCCATGGATC
    AAGCCTGCAG ATACAGAGCC ACCTCCCTTG TTGGTCTACA AGTGGTGCCA AGGCATAAAT
    AACCTGCAGG ATGTTTGGGA CACCACAGAA GGTGAATGCA ATGTGATGCT GGAGTCACGC
    TTTGAAAAGA TGTATGAAAA GATTGACTTG ACTCTGCTGA ATCGTCTGCT GCGTCTAATT
    GTTGATCACA ATATTGCTGA TTACATGACT GCAAAAAACA ACGTTGTGAT AAATTACAAG
    GATATGAACC ACACAAATTC CTATGGCATT ATTCGTGGTC TCCAGTTTGC TTCGTTTATT
    GTCCAGTACT ATGGTCTGGT GATGGATCTT CTGGTTCTTG GTTTACACAG AGCTAGTGAA
    ATGGCTGGGC CACCACAAAT GCCAAATGAC TTTCTAAGTT TCCAAGATGT GGCAACAGAA
    TCGGCCCATC CTATCCGCCT GTTTTGTCGA TACATTGACC GCATTCACAT TTTTTTCAGA
    TTTTCAGCTG ATGAAGCCAG AGACCTTATT CAGAGATATT TAACAGAACA TCCAGATCCC
    AATAATGAGA ATATTGTGGG CTACAACAAT AAAAAGTGTT GGCCAAGGGA TGCCAGAATG
    CGCCTCATGA AACATGATGT AAATCTGGGC CGTGCAGTGT TTTGGGATAT AAAAAATAGA
    CTGCCTCGTT CTGTCACCAC TGTGCAGTGG GAGAACAGTT TTGTGTCTGT GTACAGTAAA
    GACAATCCAA ACCTGCTGTT TAATATGTGC GGCTTTGAAT GCCGCATACT TCCCAAATGC
    CGCACAAGCT ATGAAGAGTT TACGCACAAG GATGGTGTCT GGAACCTGCA AAATGAAGTG
    ACCAAAGAAC GCACAGCTCA GTGCTTCTTA AGAGTTGATG ACGAGTCCAT GCAGAGATTT
    CACAACAGAG TCCGTCAAAT TCTTATGGCT TCCGGGTCCA CAACCTTTAC AAAAATTGTG
    AACAAGTGGA ATACAGCACT TATTGGCCTA ATGACTTATT TCCGTGAGGC TGTGGTCAAC
    ACCCAAGAAC TACTTGATCT GCTGGTGAAA TGTGAGAACA AAATCCAAAC AAGAATAAAG
    ATTGGGCTGA ACTCTAAAAT GCCAAGCAGG TTCCCGCCTG TTGTTTTTTA CACACCAAAG
    GAACTTGGAG GTTTGGGCAT GTTGTCCATG GGACATGTTT TAATTCCACA GTCTGACCTA
    AGGTGGTCTA AGCAGACAGA TGTTGGCATA ACCCATTTCC GTTCTGGCAT GAGTCACGAG
    GAGGATCAAC TGATTCCTAA TCTGTATCGT TACATTCAGC CTTGGGAAAG TGAGTTTATA
    GACTCTCAGA GAGTCTGGGC TGAGTATGCC TTAAAGAGGC AGGAGGCGAT TGCTCAAAAC
    AGACGCCTGA CGCTTGAAGA CTTAGAAGAC TCATGGGACA GGGGAATTCC ACGTATTAAT
    ACACTGTTTC AGAAAGATCG GCACACACTT GCTTATGACA AGGGTTGGAG AGTTCGCACA
    GACTTTAAAC AGTACCAGGT GCTGAAACAA AACCCGTTCT GGTGGACACA TCAGCGTCAT
    GATGGAAAGC TATGGAATCT GAACAACTAC CGCACAGATA TGATTCAGGC CCTTGGAGGG
    GTGGAGGGTA TACTGGAGCA CACGCTATTT AAAGGCACTT ACTTTCCCAC ATGGGAGGGT
    CTTTTCTGGG AAAAGGCCAG CGGCTTTGAA GAATCAATGA AATGGAAGAA ACTGACCAAT
    GCTCAGCGTT CTGGTTTAAA CCAGATACCA AACAGAAGAT TTACACTTTG GTGGTCTCCC
    ACAATCAACA GAGCCAACGT CTATGTTGGA TTCCAGGTGC AACTGGATTT GACTGGGATT
    TTCATGCATG GCAAGATTCC TACATTAAAA ATCTCATTGA TTCAGATATT CAGGGCCCAC
    TTGTGGCAAA AGATTCATGA AAGCATTGTC ATGGATTTAT GTCAGGTGTT TGATCAGGAG
    CTGGATGCTT TGGAGATTGA GACTGTGCAG AAAGAGACCA TCCACCCTAG GAAGTCCTAT
    AAGATGAACT CGTCCTGTGC TGATATTCTG CTCTTTGCCT CCTACAAATG GAATGTGTCT
    CGTCCCTCCT TGCTTGCTGA CTCAAAGGAT GTAATGGACA GCACAACCAC CCAGAAGTAC
    TGGATTGATA TACAGCTGAG ATGGGGTGAC TATGACTCCC ACGACATTGA AAGATATGCA
    CGTGCCAAAT TTTTGGATTA TACAACAGAT AACATGAGTA TCTATCCCTC ACCTACTGGT
    GTTCTTATTG CCATTGACCT TGCCTATAAC CTGCACAGTG CATATGGAAA CTGGTTCCCA
    GGGGGAAAGC CTCTAATACA GCAAGCAATG GCAAAGATAA TGAAGGCAAA CCCTGCCCTC
    TACGTGCTGC GGGAGCGCAT CCGTAAAGGA TTACAGCTTT ACTCTTCTGA GCCCACTGAG
    CCTTACCTGT CTTCTCAGAA CTATGGGGAA CTCTTCTCCA ATCAAATCAT CTGGTTTGTG
    GATGACACAA ATGTATACAG AGTCACCATC CACAAGACCT TTGAAGGTAA CCTGACAACC
    AAGCCAATCA ATGGAGCCAT TTTCATCTTT AATCCCAGGA CTGGCCAGCT CTTCTTAAAA
    ATTATTCATA CTTCTGTGTG GGCTGGACAG AAGAGATTGG GTCAGCTGGC CAAATGGAAA
    ACTGCAGAAG AAGTAGCTGC ATTGATTCGA TCTCTTCCAG TTGAGGAGCA GCCGAAACAA
    ATCATTGTCA CCAGGAAAGG CATGTTGGAT CCACTCGAGG TGCACTTGCT TGACTTCCCA
    AACATTGTCA TCAAAGGATC AGAATTACAG TTGCCTTTCC AGGCCTGTCT GAAGGTGGAG
    AAATTTGGTG ATCTGATCTT AAAGGCTACA GAGCCTCAGA TGGTTCTGTT CAATCTATAT
    GATGACTGGC TGAAGACGAT CTCTTCATAC ACGGCATTCT CTCGATTAAT TTTAATTCTA
    AGAGCCTTAC ATGTCAACAA TGACCGTGCA AAAGTGATCT TGAAACCTGA CAAGACCACT
    GTCACAGAGC CCCATCATAT CTGGCCCACA CTGACAGATG AGGAATGGAT TAAGGTTGAA
    GTTCAGCTAA AGGATTTGAT TTTAGCAGAT TATGGAAAGA AGAACAACGT AAATGTGGCC
    TCACTGACAC AGTCTGAAAT CAGAGACATC ATTCTGGGTA TGGAAATTTC TGCTCCATCC
    CAGCAAAGGC AGCAGATTGC AGAAATTGAA AAGCAAACAA AAGAACAGTC TCAGCTCACT
    GCTACTCAGA CGCGCACTGT CAACAAACAC GGAGATGAGA TTATCACATC TACCACCAGC
    AACTACGAAA CACAGACGTT CTCTTCAAAG ACTGAGTGGC GAGTCAGAGC AATATCTGCT
    GCCAACCTTC ACCTCCGAAC CAATCACATC TATGTATCGT CTGATGACAT TAAGGAAACT
    GGATACACCT ACATATTGCC CAAAAATGTG CTTAAGAAAT TCATTTGCAT CTCTGATCTT
    AGAGCACAGA TTGCTGGTTA CCTGTATGGT GTGAGCCCCC CAGATAATCC CCAAGTGAAG
    GAAATCCGTT GCATTGTTAT GGTGCCCCAG TGGGGAACTC ATCAAACTGT CCACCTGCCA
    AGCCAGCTGC CCCAACATGA ATACCTTAAG GAAATGGAGC CATTAGGATG GGTTCACACT
    CAGCCAAATG AGTCCCCACA GTTGTCACCT CAAGATGTCA CCACCCATGC CAAAGTAATG
    GCTGACAATC CTGCCTGGGA TGGAGAGAAA ACCATAATCA TTACTTGCAG CTTTACTCCA
    GGTTCATGCA CGTTAACCGC TTATAAACTG ACTCCCAGTG GTTATGAATG GGGACGGCAA
    AACACAGATA AAGGAAACAA TCCCAAGGGC TACTTGCCAT CGCATTATGA GAGGGTCCAG
    ATGCTGCTGT CTGATCGCTT CCTTGGGTTC TTCATGGTGC CAGCACAAGC GTCCTGGAAC
    TACAACTTCA TGGGTGTGAG ACATGACCCA AACATGAAAT ATGAGTTACA GCTGGCCAAC
    CCGAAGGAGT TTTACCATGA AGTACACCGG CCATCGCATT TCCTAAACTT TGCTCTGCTG
    CAGGAGGGTG AGGTGTATTC GGCTGACCGG GAAGATCTGT ATGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa05611
    Clone ID Related Accession (Same CDS sequence) XM_002936059.2 , XM_004911666.3
    Accession Version XM_002936059.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7008bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pre-mRNA-processing-splicing factor 8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 18, 2013 this sequence version replaced XM_002936059.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCCGGGG TGTTTCCATA TAGACCACAG GCACCGCCAA TGCCCGGCCC CCCAAGTGGA 
    ACACCGGACT ACATGTCTGA AGAGAAGCTG CAGGAAAAAG CACGAAAATG GCAGCAGCTA
    CAGGCAAAAC GCTATTCAGA GAAAAGAAAA TTTGGGTTTG TGGATGCACA GAAAGAAGAT
    ATGCCTCCAG AACATGTTCG CAAAATTATT CGTGATCATG GAGACATGAC CAACAGAAAG
    TTCCGACATG ATAAGAGAGT TTACCTTGGA GCATTGAAGT ATATGCCACA TGCTGTCTTA
    AAGTTACTTG AAAACATGCC CATGCCTTGG GAGCAGATCC GTGATGTCCC TGTGCTGTAT
    CATATAACAG GAGCCATCTC ATTTGTTAAT GAGATTCCAT GGGTCATTGA GCCTGTCTAC
    ATAGCACAGT GGGGGTCAAT GTGGATTATG ATGAGGCGAG AAAAAAGAGA CAGACGACAC
    TTCAAGAGGA TGAGGTTTCC TCCATTTGAT GATGAGGAGC CTCCACTGGA TTATGCTGAC
    AATATCCTTG ATGTTGAACC CCTGGAAGCC ATTCAGCTTG AACTGGACCC AGAGGAAGAT
    GCTCCAGTCA CTGACTGGTT CTATGATCAC CAGCCCTTAA GAGACAACAG AAAGTATGTG
    AATGGTACTA CTTATCAGCG GTGGCAGTTT ACACTTCCGA TGATGTCTAC ATTATATCGA
    TTGGCTAATC AGCTTCTGAC TGACCTGGTT GATGATAACT ACTTCTACCT GTTTGACCTA
    AAGGCATTCT TTACCTCCAA AGCATTAAAC ATGGCTATTC CTGGAGGGCC AAAATTTGAG
    CCTCTTGTTC GTGACATTAA CCTGCAGGAT GAAGATTGGA ATGAGTTTAA TGATATTAAC
    AAGATAATTA TCCGACAGCC AATTCGAACA GAATACAAGA TTGCTTTTCC TTACCTGTAC
    AACAATCTTC CTCACCATGT ACATTTAACC TGGTATCACA CACCAAATGT GGTTTTTATC
    AAAACTGAGG ATCCTGATTT ACCAGCATTC TATTTTGACC CACTGATTAA TCCCATATCA
    CACAGGCATT CTGTAAAGAG CCAAGAGCCA TTGCCAGATG ATGATGAGGA ATTTGAATTG
    CCAGAATATG TTGAACCATT CCTTAAAGAC ACTCCTCTGT ATTCAGATAA CACAGCTAAT
    GGAATTGCTT TACTTTGGGC TCCTCGTCCT TTCAACTTGC GTTCTGGTAG GACCAGGAGG
    GCTATTGATA TTCCTCTTGT GAAAAACTGG TACAGAGAGC ATTGTCCTGC TGGCCAGCCA
    GTAAAAGTCA GAGTATCTTA CCAGAAATTG CTGAAGTACT ATGTGCTAAA TGCTTTGAAA
    CACAGGCCTC CAAAAGCTCA GAAGAAAAGG TATCTCTTCC GTTCATTTAA AGCCACAAAA
    TTCTTTCAGT CGACAAAGTT GGACTGGGTG GAAGTAGGCC TGCAGGTTTG CCGCCAAGGC
    TATAACATGC TGAACTTGCT GATTCACAGA AAGAACCTTA ACTATCTTCA TCTGGATTAC
    AATTTCAATT TGAAGCCAGT AAAAACGCTG ACCACAAAGG AACGCAAAAA GTCTCGGTTC
    GGCAATGCCT TCCATCTTTG CCGCGAGGTC CTGCGTCTAA CCAAACTAGT TGTGGACAGC
    CATGTGCAAT ACAGACTGGG GAATGTTGAT GCTTTCCAGT TGGCAGACGG ATTACAGTAT
    ATTTTTGCCC ATGTGGGGCA GCTGACTGGA ATGTACCGTT ACAAGTACAA ACTAATGAGA
    CAAATTCGCA TGTGCAAGGA CCTGAAGCAT CTGATCTACT ACAGGTTCAA TACAGGTCCA
    GTTGGAAAGG GGCCTGGCTG TGGATTTTGG GCAGCAGGCT GGAGAGTCTG GCTCTTTTTT
    ATGCGTGGTA TCACACCTTT GCTGGAAAGG TGGCTTGGAA ATCTGCTTGC TAGACAATTT
    GAAGGTCGCC ATTCCAAAGG AGTTGCGAAA ACTGTGACAA AACAGCGAGT GGAGTCTCAC
    TTTGACTTGG AGCTCAGAGC TGCTGTTATG CATGACATAT TGGACATGAT GCCAGAGGGA
    ATCAAGCAGA ACAAAGCCAG AACCATCTTG CAGCACTTGA GTGAAGCTTG GAGATGCTGG
    AAAGCTAACA TTCCCTGGAA GGTACCAGGT CTTCCAACAC CTATTGAAAA CATGATTCTG
    CGATATGTAA AGGCCAAAGC GGACTGGTGG ACAAATACTG CGCATTACAA CAGAGAACGT
    ATTAGACGTG GTGCCACTGT CGACAAAACT GTGTGTAAGA AGAATCTGGG CCGTCTGACT
    CGTTTGTACT TGAAAGCAGA ACAAGAGAGA CAGCACAACT ACCTGAAGGA TGGGCCTTAC
    ATTACTGCAG AGGAGGCAGT TGCTGTGTAC ACTACCACTG TCCACTGGCT AGAGAGTAGG
    AGGTTTTCTC CAATTCCTTT TCCTCCTCTT TCCTACAAAC ATGATACAAA ACTACTCATT
    CTAGCACTTG AGAGACTGAA GGAAGCATAC AGTGTGAAGT CGCGTTTGAA TCAGTCTCAG
    AGGGAAGAAT TGGGTTTAAT CGAACAGGCG TATGACAACC CTCACGAAGC TCTGTCCAGA
    ATCAAGCGTC ACTTGCTCAC CCAAAGAGCT TTCAAGGAGG TTGGAATAGA GTTTATGGAT
    CTCTACAGCC ACCTTGTTCC TGTTTATGAC GTGGAACCAC TGGAAAAAAT TACTGATGCC
    TACCTGGATC AGTACCTGTG GTATGAAGCA GATAAAAGAA GGCTTTTCCC TCCATGGATC
    AAGCCTGCAG ATACAGAGCC ACCTCCCTTG TTGGTCTACA AGTGGTGCCA AGGCATAAAT
    AACCTGCAGG ATGTTTGGGA CACCACAGAA GGTGAATGCA ATGTGATGCT GGAGTCACGC
    TTTGAAAAGA TGTATGAAAA GATTGACTTG ACTCTGCTGA ATCGTCTGCT GCGTCTAATT
    GTTGATCACA ATATTGCTGA TTACATGACT GCAAAAAACA ACGTTGTGAT AAATTACAAG
    GATATGAACC ACACAAATTC CTATGGCATT ATTCGTGGTC TCCAGTTTGC TTCGTTTATT
    GTCCAGTACT ATGGTCTGGT GATGGATCTT CTGGTTCTTG GTTTACACAG AGCTAGTGAA
    ATGGCTGGGC CACCACAAAT GCCAAATGAC TTTCTAAGTT TCCAAGATGT GGCAACAGAA
    TCGGCCCATC CTATCCGCCT GTTTTGTCGA TACATTGACC GCATTCACAT TTTTTTCAGA
    TTTTCAGCTG ATGAAGCCAG AGACCTTATT CAGAGATATT TAACAGAACA TCCAGATCCC
    AATAATGAGA ATATTGTGGG CTACAACAAT AAAAAGTGTT GGCCAAGGGA TGCCAGAATG
    CGCCTCATGA AACATGATGT AAATCTGGGC CGTGCAGTGT TTTGGGATAT AAAAAATAGA
    CTGCCTCGTT CTGTCACCAC TGTGCAGTGG GAGAACAGTT TTGTGTCTGT GTACAGTAAA
    GACAATCCAA ACCTGCTGTT TAATATGTGC GGCTTTGAAT GCCGCATACT TCCCAAATGC
    CGCACAAGCT ATGAAGAGTT TACGCACAAG GATGGTGTCT GGAACCTGCA AAATGAAGTG
    ACCAAAGAAC GCACAGCTCA GTGCTTCTTA AGAGTTGATG ACGAGTCCAT GCAGAGATTT
    CACAACAGAG TCCGTCAAAT TCTTATGGCT TCCGGGTCCA CAACCTTTAC AAAAATTGTG
    AACAAATGGA ATACAGCACT TATTGGCCTA ATGACTTATT TCCGTGAGGC TGTGGTCAAC
    ACCCAAGAAC TACTTGATCT GCTGGTGAAA TGTGAGAACA AAATCCAAAC AAGAATAAAG
    ATTGGGCTGA ACTCTAAAAT GCCAAGCAGG TTCCCGCCTG TTGTTTTTTA CACACCAAAG
    GAACTTGGAG GTTTGGGCAT GTTGTCCATG GGACATGTTT TAATTCCACA GTCTGACCTA
    AGGTGGTCTA AGCAGACAGA TGTTGGCATA ACCCATTTCC GTTCTGGCAT GAGTCATGAG
    GAGGATCAAC TGATTCCTAA TCTGTATCGT TACATTCAGC CTTGGGAAAG TGAGTTTATA
    GACTCTCAGA GAGTCTGGGC TGAGTATGCC TTAAAGAGGC AGGAGGCGAT TGCTCAAAAC
    AGACGCCTGA CGCTTGAAGA CTTAGAAGAC TCATGGGACA GGGGAATTCC ACGTATTAAT
    ACACTGTTTC AGAAAGATCG GCACACACTT GCTTATGACA AGGGTTGGAG AGTTCGCACA
    GACTTTAAAC AGTACCAGGT GCTGAAACAA AATCCGTTCT GGTGGACACA TCAGCGTCAT
    GATGGAAAGC TATGGAATCT GAACAACTAC CGCACAGATA TGATTCAGGC CCTTGGAGGG
    GTGGAGGGTA TACTGGAGCA CACGCTATTT AAAGGCACTT ACTTTCCCAC ATGGGAGGGT
    CTTTTCTGGG AAAAGGCCAG CGGCTTTGAA GAATCAATGA AATGGAAGAA ACTGACCAAT
    GCTCAGCGTT CTGGTTTAAA CCAGATACCA AACAGAAGAT TTACACTTTG GTGGTCTCCC
    ACAATCAACA GAGCCAACGT CTATGTTGGA TTCCAGGTGC AACTGGATTT GACTGGGATT
    TTCATGCATG GCAAGATTCC TACATTAAAA ATCTCATTGA TTCAGATATT CAGGGCCCAC
    TTGTGGCAAA AGATTCATGA AAGCATTGTC ATGGATTTAT GTCAGGTGTT TGATCAGGAG
    CTGGATGCTT TGGAGATTGA GACTGTGCAG AAAGAGACCA TCCACCCTAG GAAGTCCTAT
    AAGATGAACT CGTCCTGTGC TGATATTCTG CTCTTTGCCT CCTACAAATG GAATGTGTCT
    CGTCCCTCCT TGCTTGCTGA CTCAAAGGAT GTAATGGACA GCACAACCAC CCAGAAGTAC
    TGGATTGATA TACAGCTGAG ATGGGGTGAC TATGACTCCC ACGACATTGA AAGATATGCA
    CGTGCCAAAT TTTTGGATTA TACAACGGAT AACATGAGTA TCTATCCCTC ACCTACTGGT
    GTTCTTATTG CCATTGACCT TGCCTATAAC CTGCACAGTG CATATGGAAA CTGGTTCCCA
    GGGGGAAAGC CTCTAATACA GCAAGCAATG GCAAAGATAA TGAAGGCAAA CCCTGCCCTC
    TACGTGCTGC GGGAGCGCAT CCGTAAAGGA TTACAGCTTT ACTCTTCTGA GCCCACTGAG
    CCTTACCTGT CTTCTCAGAA CTATGGGGAA CTCTTCTCCA ATCAAATCAT CTGGTTTGTG
    GATGACACAA ATGTATACAG AGTCACCATC CACAAGACCT TTGAAGGTAA CCTGACAACC
    AAGCCAATCA ATGGAGCCAT TTTCATCTTT AATCCCAGGA CTGGCCAGCT CTTCTTAAAA
    ATTATTCATA CTTCTGTGTG GGCTGGACAG AAGAGATTGG GTCAGCTGGC CAAATGGAAA
    ACGGCAGAAG AAGTAGCTGC ATTGATTCGA TCTCTTCCAG TTGAGGAGCA GCCGAAACAA
    ATCATTGTCA CCAGGAAAGG CATGTTGGAT CCACTCGAGG TGCACTTGCT TGACTTCCCA
    AACATTGTCA TCAAAGGATC AGAATTACAG TTGCCTTTCC AGGCCTGTCT GAAGGTGGAG
    AAATTTGGTG ATCTGATCTT AAAGGCTACA GAGCCTCAGA TGGTTCTGTT CAATCTATAT
    GATGACTGGC TGAAGACGAT CTCTTCATAC ACGGCATTCT CTCGATTAAT TTTAATTCTA
    AGAGCCTTAC ATGTCAACAA TGACCGTGCA AAAGTGATCT TGAAACCTGA CAAGACCACT
    GTCACAGAGC CCCATCATAT CTGGCCCACA CTGACAGATG AGGAATGGAT TAAGGTTGAA
    GTTCAGCTAA AGGATTTGAT TTTAGCAGAT TATGGAAAGA AGAACAACGT AAATGTGGCC
    TCACTGACAC AGTCTGAAAT CAGAGACATC ATTCTGGGTA TGGAAATATC TGCTCCATCC
    CAGCAAAGGC AGCAGATTGC AGAAATTGAA AAGCAAACAA AAGAACAGTC TCAGCTCACT
    GCTACTCAGA CGCGCACTGT CAACAAACAC GGAGATGAGA TTATCACATC TACCACCAGC
    AACTACGAAA CACAGACGTT CTCTTCAAAG ACTGAGTGGC GAGTCAGAGC AATATCTGCT
    GCCAACCTTC ACCTCCGAAC CAATCACATC TATGTATCGT CTGATGACAT TAAGGAAACT
    GGATACACCT ACATATTGCC CAAAAATGTG CTTAAGAAAT TCATTTGCAT CTCTGATCTT
    AGAGCACAGA TTGCTGGTTA CCTGTATGGT GTGAGCCCCC CAGATAATCC CCAAGTGAAG
    GAAATCCGTT GCATTGTTAT GGTGCCCCAG TGGGGAACTC ATCAAACTGT CCACCTGCCA
    AGCCAGCTGC CCCAACATGA ATACCTTAAG GAAATGGAGC CATTAGGATG GGTTCACACT
    CAGCCAAATG AGTCCCCACA GTTGTCACCT CAAGATGTCA CCACCCATGC CAAAGTAATG
    GCTGACAATC CTGCCTGGGA TGGAGAGAAA ACCATAATCA TTACTTGCAG CTTTACTCCA
    GGTTCATGCA CGTTAACCGC TTATAAACTG ACTCCCAGTG GTTATGAATG GGGACGGCAA
    AACACAGATA AAGGAAACAA TCCCAAGGGC TACTTGCCAT CGCATTATGA GAGGGTCCAG
    ATGCTGCTGT CTGATCGCTT CCTTGGGTTC TTCATGGTGC CAGCACAAGC GTCCTGGAAC
    TACAACTTCA TGGGTGTGAG ACATGACCCA AACATGAAAT ATGAGTTACA GCTGGCCAAC
    CCGAAGGAGT TTTACCATGA AGTACACCGG CCATCGCATT TCCTAAACTT TGCTCTGCTG
    CAGGAGGGTG AGGTGTATTC GGCTGACCGG GAAGATCTGT ATGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002936105.1
    CDS243..7250
    Translation

    Target ORF information:

    RefSeq Version XM_002936059.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pre-mRNA processing factor 8 (prpf8), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002936059.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCCGGGG TGTTTCCATA TAGACCACAG GCACCGCCAA TGCCCGGCCC CCCAAGTGGA 
    ACACCGGACT ACATGTCTGA AGAGAAGCTG CAGGAAAAAG CACGAAAATG GCAGCAGCTA
    CAGGCAAAAC GCTATTCAGA GAAAAGAAAA TTTGGGTTTG TGGATGCACA GAAAGAAGAT
    ATGCCTCCAG AACATGTTCG CAAAATTATT CGTGATCATG GAGACATGAC CAACAGAAAG
    TTCCGACATG ATAAGAGAGT TTACCTTGGA GCATTGAAGT ATATGCCACA TGCTGTCTTA
    AAGTTACTTG AAAACATGCC CATGCCTTGG GAGCAGATCC GTGATGTCCC TGTGCTGTAT
    CATATAACAG GAGCCATCTC ATTTGTTAAT GAGATTCCAT GGGTCATTGA GCCTGTCTAC
    ATAGCACAGT GGGGGTCAAT GTGGATTATG ATGAGGCGAG AAAAAAGAGA CAGACGACAC
    TTCAAGAGGA TGAGGTTTCC TCCATTTGAT GATGAGGAGC CTCCACTGGA TTATGCTGAC
    AATATCCTTG ATGTTGAACC CCTGGAAGCC ATTCAGCTTG AACTGGACCC AGAGGAAGAT
    GCTCCAGTCA CTGACTGGTT CTATGATCAC CAGCCCTTAA GAGACAACAG AAAGTATGTG
    AATGGTACTA CTTATCAGCG GTGGCAGTTT ACACTTCCGA TGATGTCTAC ATTATATCGA
    TTGGCTAATC AGCTTCTGAC TGACCTGGTT GATGATAACT ACTTCTACCT GTTTGACCTA
    AAGGCATTCT TTACCTCCAA AGCATTAAAC ATGGCTATTC CTGGAGGGCC AAAATTTGAG
    CCTCTTGTTC GTGACATTAA CCTGCAGGAT GAAGATTGGA ATGAGTTTAA TGATATTAAC
    AAGATAATTA TCCGACAGCC AATTCGAACA GAATACAAGA TTGCTTTTCC TTACCTGTAC
    AACAATCTTC CTCACCATGT ACATTTAACC TGGTATCACA CACCAAATGT GGTTTTTATC
    AAAACTGAGG ATCCTGATTT ACCAGCATTC TATTTTGACC CACTGATTAA TCCCATATCA
    CACAGGCATT CTGTAAAGAG CCAAGAGCCA TTGCCAGATG ATGATGAGGA ATTTGAATTG
    CCAGAATATG TTGAACCATT CCTTAAAGAC ACTCCTCTGT ATTCAGATAA CACAGCTAAT
    GGAATTGCTT TACTTTGGGC TCCTCGTCCT TTCAACTTGC GTTCTGGTAG GACCAGGAGG
    GCTATTGATA TTCCTCTTGT GAAAAACTGG TACAGAGAGC ATTGTCCTGC TGGCCAGCCA
    GTAAAAGTCA GAGTATCTTA CCAGAAATTG CTGAAGTACT ATGTGCTAAA TGCTTTGAAA
    CACAGGCCTC CAAAAGCTCA GAAGAAAAGG TATCTCTTCC GTTCATTTAA AGCCACAAAA
    TTCTTTCAGT CGACAAAGTT GGACTGGGTG GAAGTAGGCC TGCAGGTTTG CCGCCAAGGC
    TATAACATGC TGAACTTGCT GATTCACAGA AAGAACCTTA ACTATCTTCA TCTGGATTAC
    AATTTCAATT TGAAGCCAGT AAAAACGCTG ACCACAAAGG AACGCAAAAA GTCTCGGTTC
    GGCAATGCCT TCCATCTTTG CCGCGAGGTC CTGCGTCTAA CCAAACTAGT TGTGGACAGC
    CATGTGCAAT ACAGACTGGG GAATGTTGAT GCTTTCCAGT TGGCAGACGG ATTACAGTAT
    ATTTTTGCCC ATGTGGGGCA GCTGACTGGA ATGTACCGTT ACAAGTACAA ACTAATGAGA
    CAAATTCGCA TGTGCAAGGA CCTGAAGCAT CTGATCTACT ACAGGTTCAA TACAGGTCCA
    GTTGGAAAGG GGCCTGGCTG TGGATTTTGG GCAGCAGGCT GGAGAGTCTG GCTCTTTTTT
    ATGCGTGGTA TCACACCTTT GCTGGAAAGG TGGCTTGGAA ATCTGCTTGC TAGACAATTT
    GAAGGTCGCC ATTCCAAAGG AGTTGCGAAA ACTGTGACAA AACAGCGAGT GGAGTCTCAC
    TTTGACTTGG AGCTCAGAGC TGCTGTTATG CATGACATAT TGGACATGAT GCCAGAGGGA
    ATCAAGCAGA ACAAAGCCAG AACCATCTTG CAGCACTTGA GTGAAGCTTG GAGATGCTGG
    AAAGCTAACA TTCCCTGGAA GGTACCAGGT CTTCCAACAC CTATTGAAAA CATGATTCTG
    CGATATGTAA AGGCCAAAGC GGACTGGTGG ACAAATACTG CGCATTACAA CAGAGAACGT
    ATTAGACGTG GTGCCACTGT CGACAAAACT GTGTGTAAGA AGAATCTGGG CCGTCTGACT
    CGTTTGTACT TGAAAGCAGA ACAAGAGAGA CAGCACAACT ACCTGAAGGA TGGGCCTTAC
    ATTACTGCAG AGGAGGCAGT TGCTGTGTAC ACTACCACTG TCCACTGGCT AGAGAGTAGG
    AGGTTTTCTC CAATTCCTTT TCCTCCTCTT TCCTACAAAC ATGATACAAA ACTACTCATT
    CTAGCACTTG AGAGACTGAA GGAAGCATAC AGTGTGAAGT CGCGTTTGAA TCAGTCTCAG
    AGGGAAGAAT TGGGTTTAAT CGAACAGGCG TATGACAACC CTCACGAAGC TCTGTCCAGA
    ATCAAGCGTC ACTTGCTCAC CCAAAGAGCT TTCAAGGAGG TTGGAATAGA GTTTATGGAT
    CTCTACAGCC ACCTTGTTCC TGTTTATGAC GTGGAACCAC TGGAAAAAAT TACTGATGCC
    TACCTGGATC AGTACCTGTG GTATGAAGCA GATAAAAGAA GGCTTTTCCC TCCATGGATC
    AAGCCTGCAG ATACAGAGCC ACCTCCCTTG TTGGTCTACA AGTGGTGCCA AGGCATAAAT
    AACCTGCAGG ATGTTTGGGA CACCACAGAA GGTGAATGCA ATGTGATGCT GGAGTCACGC
    TTTGAAAAGA TGTATGAAAA GATTGACTTG ACTCTGCTGA ATCGTCTGCT GCGTCTAATT
    GTTGATCACA ATATTGCTGA TTACATGACT GCAAAAAACA ACGTTGTGAT AAATTACAAG
    GATATGAACC ACACAAATTC CTATGGCATT ATTCGTGGTC TCCAGTTTGC TTCGTTTATT
    GTCCAGTACT ATGGTCTGGT GATGGATCTT CTGGTTCTTG GTTTACACAG AGCTAGTGAA
    ATGGCTGGGC CACCACAAAT GCCAAATGAC TTTCTAAGTT TCCAAGATGT GGCAACAGAA
    TCGGCCCATC CTATCCGCCT GTTTTGTCGA TACATTGACC GCATTCACAT TTTTTTCAGA
    TTTTCAGCTG ATGAAGCCAG AGACCTTATT CAGAGATATT TAACAGAACA TCCAGATCCC
    AATAATGAGA ATATTGTGGG CTACAACAAT AAAAAGTGTT GGCCAAGGGA TGCCAGAATG
    CGCCTCATGA AACATGATGT AAATCTGGGC CGTGCAGTGT TTTGGGATAT AAAAAATAGA
    CTGCCTCGTT CTGTCACCAC TGTGCAGTGG GAGAACAGTT TTGTGTCTGT GTACAGTAAA
    GACAATCCAA ACCTGCTGTT TAATATGTGC GGCTTTGAAT GCCGCATACT TCCCAAATGC
    CGCACAAGCT ATGAAGAGTT TACGCACAAG GATGGTGTCT GGAACCTGCA AAATGAAGTG
    ACCAAAGAAC GCACAGCTCA GTGCTTCTTA AGAGTTGATG ACGAGTCCAT GCAGAGATTT
    CACAACAGAG TCCGTCAAAT TCTTATGGCT TCCGGGTCCA CAACCTTTAC AAAAATTGTG
    AACAAATGGA ATACAGCACT TATTGGCCTA ATGACTTATT TCCGTGAGGC TGTGGTCAAC
    ACCCAAGAAC TACTTGATCT GCTGGTGAAA TGTGAGAACA AAATCCAAAC AAGAATAAAG
    ATTGGGCTGA ACTCTAAAAT GCCAAGCAGG TTCCCGCCTG TTGTTTTTTA CACACCAAAG
    GAACTTGGAG GTTTGGGCAT GTTGTCCATG GGACATGTTT TAATTCCACA GTCTGACCTA
    AGGTGGTCTA AGCAGACAGA TGTTGGCATA ACCCATTTCC GTTCTGGCAT GAGTCATGAG
    GAGGATCAAC TGATTCCTAA TCTGTATCGT TACATTCAGC CTTGGGAAAG TGAGTTTATA
    GACTCTCAGA GAGTCTGGGC TGAGTATGCC TTAAAGAGGC AGGAGGCGAT TGCTCAAAAC
    AGACGCCTGA CGCTTGAAGA CTTAGAAGAC TCATGGGACA GGGGAATTCC ACGTATTAAT
    ACACTGTTTC AGAAAGATCG GCACACACTT GCTTATGACA AGGGTTGGAG AGTTCGCACA
    GACTTTAAAC AGTACCAGGT GCTGAAACAA AATCCGTTCT GGTGGACACA TCAGCGTCAT
    GATGGAAAGC TATGGAATCT GAACAACTAC CGCACAGATA TGATTCAGGC CCTTGGAGGG
    GTGGAGGGTA TACTGGAGCA CACGCTATTT AAAGGCACTT ACTTTCCCAC ATGGGAGGGT
    CTTTTCTGGG AAAAGGCCAG CGGCTTTGAA GAATCAATGA AATGGAAGAA ACTGACCAAT
    GCTCAGCGTT CTGGTTTAAA CCAGATACCA AACAGAAGAT TTACACTTTG GTGGTCTCCC
    ACAATCAACA GAGCCAACGT CTATGTTGGA TTCCAGGTGC AACTGGATTT GACTGGGATT
    TTCATGCATG GCAAGATTCC TACATTAAAA ATCTCATTGA TTCAGATATT CAGGGCCCAC
    TTGTGGCAAA AGATTCATGA AAGCATTGTC ATGGATTTAT GTCAGGTGTT TGATCAGGAG
    CTGGATGCTT TGGAGATTGA GACTGTGCAG AAAGAGACCA TCCACCCTAG GAAGTCCTAT
    AAGATGAACT CGTCCTGTGC TGATATTCTG CTCTTTGCCT CCTACAAATG GAATGTGTCT
    CGTCCCTCCT TGCTTGCTGA CTCAAAGGAT GTAATGGACA GCACAACCAC CCAGAAGTAC
    TGGATTGATA TACAGCTGAG ATGGGGTGAC TATGACTCCC ACGACATTGA AAGATATGCA
    CGTGCCAAAT TTTTGGATTA TACAACGGAT AACATGAGTA TCTATCCCTC ACCTACTGGT
    GTTCTTATTG CCATTGACCT TGCCTATAAC CTGCACAGTG CATATGGAAA CTGGTTCCCA
    GGGGGAAAGC CTCTAATACA GCAAGCAATG GCAAAGATAA TGAAGGCAAA CCCTGCCCTC
    TACGTGCTGC GGGAGCGCAT CCGTAAAGGA TTACAGCTTT ACTCTTCTGA GCCCACTGAG
    CCTTACCTGT CTTCTCAGAA CTATGGGGAA CTCTTCTCCA ATCAAATCAT CTGGTTTGTG
    GATGACACAA ATGTATACAG AGTCACCATC CACAAGACCT TTGAAGGTAA CCTGACAACC
    AAGCCAATCA ATGGAGCCAT TTTCATCTTT AATCCCAGGA CTGGCCAGCT CTTCTTAAAA
    ATTATTCATA CTTCTGTGTG GGCTGGACAG AAGAGATTGG GTCAGCTGGC CAAATGGAAA
    ACGGCAGAAG AAGTAGCTGC ATTGATTCGA TCTCTTCCAG TTGAGGAGCA GCCGAAACAA
    ATCATTGTCA CCAGGAAAGG CATGTTGGAT CCACTCGAGG TGCACTTGCT TGACTTCCCA
    AACATTGTCA TCAAAGGATC AGAATTACAG TTGCCTTTCC AGGCCTGTCT GAAGGTGGAG
    AAATTTGGTG ATCTGATCTT AAAGGCTACA GAGCCTCAGA TGGTTCTGTT CAATCTATAT
    GATGACTGGC TGAAGACGAT CTCTTCATAC ACGGCATTCT CTCGATTAAT TTTAATTCTA
    AGAGCCTTAC ATGTCAACAA TGACCGTGCA AAAGTGATCT TGAAACCTGA CAAGACCACT
    GTCACAGAGC CCCATCATAT CTGGCCCACA CTGACAGATG AGGAATGGAT TAAGGTTGAA
    GTTCAGCTAA AGGATTTGAT TTTAGCAGAT TATGGAAAGA AGAACAACGT AAATGTGGCC
    TCACTGACAC AGTCTGAAAT CAGAGACATC ATTCTGGGTA TGGAAATATC TGCTCCATCC
    CAGCAAAGGC AGCAGATTGC AGAAATTGAA AAGCAAACAA AAGAACAGTC TCAGCTCACT
    GCTACTCAGA CGCGCACTGT CAACAAACAC GGAGATGAGA TTATCACATC TACCACCAGC
    AACTACGAAA CACAGACGTT CTCTTCAAAG ACTGAGTGGC GAGTCAGAGC AATATCTGCT
    GCCAACCTTC ACCTCCGAAC CAATCACATC TATGTATCGT CTGATGACAT TAAGGAAACT
    GGATACACCT ACATATTGCC CAAAAATGTG CTTAAGAAAT TCATTTGCAT CTCTGATCTT
    AGAGCACAGA TTGCTGGTTA CCTGTATGGT GTGAGCCCCC CAGATAATCC CCAAGTGAAG
    GAAATCCGTT GCATTGTTAT GGTGCCCCAG TGGGGAACTC ATCAAACTGT CCACCTGCCA
    AGCCAGCTGC CCCAACATGA ATACCTTAAG GAAATGGAGC CATTAGGATG GGTTCACACT
    CAGCCAAATG AGTCCCCACA GTTGTCACCT CAAGATGTCA CCACCCATGC CAAAGTAATG
    GCTGACAATC CTGCCTGGGA TGGAGAGAAA ACCATAATCA TTACTTGCAG CTTTACTCCA
    GGTTCATGCA CGTTAACCGC TTATAAACTG ACTCCCAGTG GTTATGAATG GGGACGGCAA
    AACACAGATA AAGGAAACAA TCCCAAGGGC TACTTGCCAT CGCATTATGA GAGGGTCCAG
    ATGCTGCTGT CTGATCGCTT CCTTGGGTTC TTCATGGTGC CAGCACAAGC GTCCTGGAAC
    TACAACTTCA TGGGTGTGAG ACATGACCCA AACATGAAAT ATGAGTTACA GCTGGCCAAC
    CCGAAGGAGT TTTACCATGA AGTACACCGG CCATCGCATT TCCTAAACTT TGCTCTGCTG
    CAGGAGGGTG AGGTGTATTC GGCTGACCGG GAAGATCTGT ATGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa05611
    Clone ID Related Accession (Same CDS sequence) XM_002936059.2 , XM_004911666.3
    Accession Version XM_004911666.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7008bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product pre-mRNA-processing-splicing factor 8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004911666.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
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    1981
    2041
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    3001
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    3181
    3241
    3301
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    3421
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    3721
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    3901
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    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
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    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCCGGGG TGTTTCCATA TAGACCACAG GCACCGCCAA TGCCCGGCCC CCCAAGTGGA 
    ACACCGGACT ACATGTCTGA AGAGAAGCTG CAGGAAAAAG CACGAAAATG GCAGCAGCTA
    CAGGCAAAAC GCTATTCAGA GAAAAGAAAA TTTGGGTTTG TGGATGCACA GAAAGAAGAT
    ATGCCTCCAG AACATGTTCG CAAAATTATT CGTGATCATG GAGACATGAC CAACAGAAAG
    TTCCGACATG ATAAGAGAGT TTACCTTGGA GCATTGAAGT ATATGCCACA TGCTGTCTTA
    AAGTTACTTG AAAACATGCC CATGCCTTGG GAGCAGATCC GTGATGTCCC TGTGCTGTAT
    CATATAACAG GAGCCATCTC ATTTGTTAAT GAGATTCCAT GGGTCATTGA GCCTGTCTAC
    ATAGCACAGT GGGGGTCAAT GTGGATTATG ATGAGGCGAG AAAAAAGAGA CAGACGACAC
    TTCAAGAGGA TGAGGTTTCC TCCATTTGAT GATGAGGAGC CTCCACTGGA TTATGCTGAC
    AATATCCTTG ATGTTGAACC CCTGGAAGCC ATTCAGCTTG AACTGGACCC AGAGGAAGAT
    GCTCCAGTCA CTGACTGGTT CTATGATCAC CAGCCCTTAA GAGACAACAG AAAGTATGTG
    AATGGTACTA CTTATCAGCG GTGGCAGTTT ACACTTCCGA TGATGTCTAC ATTATATCGA
    TTGGCTAATC AGCTTCTGAC TGACCTGGTT GATGATAACT ACTTCTACCT GTTTGACCTA
    AAGGCATTCT TTACCTCCAA AGCATTAAAC ATGGCTATTC CTGGAGGGCC AAAATTTGAG
    CCTCTTGTTC GTGACATTAA CCTGCAGGAT GAAGATTGGA ATGAGTTTAA TGATATTAAC
    AAGATAATTA TCCGACAGCC AATTCGAACA GAATACAAGA TTGCTTTTCC TTACCTGTAC
    AACAATCTTC CTCACCATGT ACATTTAACC TGGTATCACA CACCAAATGT GGTTTTTATC
    AAAACTGAGG ATCCTGATTT ACCAGCATTC TATTTTGACC CACTGATTAA TCCCATATCA
    CACAGGCATT CTGTAAAGAG CCAAGAGCCA TTGCCAGATG ATGATGAGGA ATTTGAATTG
    CCAGAATATG TTGAACCATT CCTTAAAGAC ACTCCTCTGT ATTCAGATAA CACAGCTAAT
    GGAATTGCTT TACTTTGGGC TCCTCGTCCT TTCAACTTGC GTTCTGGTAG GACCAGGAGG
    GCTATTGATA TTCCTCTTGT GAAAAACTGG TACAGAGAGC ATTGTCCTGC TGGCCAGCCA
    GTAAAAGTCA GAGTATCTTA CCAGAAATTG CTGAAGTACT ATGTGCTAAA TGCTTTGAAA
    CACAGGCCTC CAAAAGCTCA GAAGAAAAGG TATCTCTTCC GTTCATTTAA AGCCACAAAA
    TTCTTTCAGT CGACAAAGTT GGACTGGGTG GAAGTAGGCC TGCAGGTTTG CCGCCAAGGC
    TATAACATGC TGAACTTGCT GATTCACAGA AAGAACCTTA ACTATCTTCA TCTGGATTAC
    AATTTCAATT TGAAGCCAGT AAAAACGCTG ACCACAAAGG AACGCAAAAA GTCTCGGTTC
    GGCAATGCCT TCCATCTTTG CCGCGAGGTC CTGCGTCTAA CCAAACTAGT TGTGGACAGC
    CATGTGCAAT ACAGACTGGG GAATGTTGAT GCTTTCCAGT TGGCAGACGG ATTACAGTAT
    ATTTTTGCCC ATGTGGGGCA GCTGACTGGA ATGTACCGTT ACAAGTACAA ACTAATGAGA
    CAAATTCGCA TGTGCAAGGA CCTGAAGCAT CTGATCTACT ACAGGTTCAA TACAGGTCCA
    GTTGGAAAGG GGCCTGGCTG TGGATTTTGG GCAGCAGGCT GGAGAGTCTG GCTCTTTTTT
    ATGCGTGGTA TCACACCTTT GCTGGAAAGG TGGCTTGGAA ATCTGCTTGC TAGACAATTT
    GAAGGTCGCC ATTCCAAAGG AGTTGCGAAA ACTGTGACAA AACAGCGAGT GGAGTCTCAC
    TTTGACTTGG AGCTCAGAGC TGCTGTTATG CATGACATAT TGGACATGAT GCCAGAGGGA
    ATCAAGCAGA ACAAAGCCAG AACCATCTTG CAGCACTTGA GTGAAGCTTG GAGATGCTGG
    AAAGCTAACA TTCCCTGGAA GGTACCAGGT CTTCCAACAC CTATTGAAAA CATGATTCTG
    CGATATGTAA AGGCCAAAGC GGACTGGTGG ACAAATACTG CGCATTACAA CAGAGAACGT
    ATTAGACGTG GTGCCACTGT CGACAAAACT GTGTGTAAGA AGAATCTGGG CCGTCTGACT
    CGTTTGTACT TGAAAGCAGA ACAAGAGAGA CAGCACAACT ACCTGAAGGA TGGGCCTTAC
    ATTACTGCAG AGGAGGCAGT TGCTGTGTAC ACTACCACTG TCCACTGGCT AGAGAGTAGG
    AGGTTTTCTC CAATTCCTTT TCCTCCTCTT TCCTACAAAC ATGATACAAA ACTACTCATT
    CTAGCACTTG AGAGACTGAA GGAAGCATAC AGTGTGAAGT CGCGTTTGAA TCAGTCTCAG
    AGGGAAGAAT TGGGTTTAAT CGAACAGGCG TATGACAACC CTCACGAAGC TCTGTCCAGA
    ATCAAGCGTC ACTTGCTCAC CCAAAGAGCT TTCAAGGAGG TTGGAATAGA GTTTATGGAT
    CTCTACAGCC ACCTTGTTCC TGTTTATGAC GTGGAACCAC TGGAAAAAAT TACTGATGCC
    TACCTGGATC AGTACCTGTG GTATGAAGCA GATAAAAGAA GGCTTTTCCC TCCATGGATC
    AAGCCTGCAG ATACAGAGCC ACCTCCCTTG TTGGTCTACA AGTGGTGCCA AGGCATAAAT
    AACCTGCAGG ATGTTTGGGA CACCACAGAA GGTGAATGCA ATGTGATGCT GGAGTCACGC
    TTTGAAAAGA TGTATGAAAA GATTGACTTG ACTCTGCTGA ATCGTCTGCT GCGTCTAATT
    GTTGATCACA ATATTGCTGA TTACATGACT GCAAAAAACA ACGTTGTGAT AAATTACAAG
    GATATGAACC ACACAAATTC CTATGGCATT ATTCGTGGTC TCCAGTTTGC TTCGTTTATT
    GTCCAGTACT ATGGTCTGGT GATGGATCTT CTGGTTCTTG GTTTACACAG AGCTAGTGAA
    ATGGCTGGGC CACCACAAAT GCCAAATGAC TTTCTAAGTT TCCAAGATGT GGCAACAGAA
    TCGGCCCATC CTATCCGCCT GTTTTGTCGA TACATTGACC GCATTCACAT TTTTTTCAGA
    TTTTCAGCTG ATGAAGCCAG AGACCTTATT CAGAGATATT TAACAGAACA TCCAGATCCC
    AATAATGAGA ATATTGTGGG CTACAACAAT AAAAAGTGTT GGCCAAGGGA TGCCAGAATG
    CGCCTCATGA AACATGATGT AAATCTGGGC CGTGCAGTGT TTTGGGATAT AAAAAATAGA
    CTGCCTCGTT CTGTCACCAC TGTGCAGTGG GAGAACAGTT TTGTGTCTGT GTACAGTAAA
    GACAATCCAA ACCTGCTGTT TAATATGTGC GGCTTTGAAT GCCGCATACT TCCCAAATGC
    CGCACAAGCT ATGAAGAGTT TACGCACAAG GATGGTGTCT GGAACCTGCA AAATGAAGTG
    ACCAAAGAAC GCACAGCTCA GTGCTTCTTA AGAGTTGATG ACGAGTCCAT GCAGAGATTT
    CACAACAGAG TCCGTCAAAT TCTTATGGCT TCCGGGTCCA CAACCTTTAC AAAAATTGTG
    AACAAATGGA ATACAGCACT TATTGGCCTA ATGACTTATT TCCGTGAGGC TGTGGTCAAC
    ACCCAAGAAC TACTTGATCT GCTGGTGAAA TGTGAGAACA AAATCCAAAC AAGAATAAAG
    ATTGGGCTGA ACTCTAAAAT GCCAAGCAGG TTCCCGCCTG TTGTTTTTTA CACACCAAAG
    GAACTTGGAG GTTTGGGCAT GTTGTCCATG GGACATGTTT TAATTCCACA GTCTGACCTA
    AGGTGGTCTA AGCAGACAGA TGTTGGCATA ACCCATTTCC GTTCTGGCAT GAGTCATGAG
    GAGGATCAAC TGATTCCTAA TCTGTATCGT TACATTCAGC CTTGGGAAAG TGAGTTTATA
    GACTCTCAGA GAGTCTGGGC TGAGTATGCC TTAAAGAGGC AGGAGGCGAT TGCTCAAAAC
    AGACGCCTGA CGCTTGAAGA CTTAGAAGAC TCATGGGACA GGGGAATTCC ACGTATTAAT
    ACACTGTTTC AGAAAGATCG GCACACACTT GCTTATGACA AGGGTTGGAG AGTTCGCACA
    GACTTTAAAC AGTACCAGGT GCTGAAACAA AATCCGTTCT GGTGGACACA TCAGCGTCAT
    GATGGAAAGC TATGGAATCT GAACAACTAC CGCACAGATA TGATTCAGGC CCTTGGAGGG
    GTGGAGGGTA TACTGGAGCA CACGCTATTT AAAGGCACTT ACTTTCCCAC ATGGGAGGGT
    CTTTTCTGGG AAAAGGCCAG CGGCTTTGAA GAATCAATGA AATGGAAGAA ACTGACCAAT
    GCTCAGCGTT CTGGTTTAAA CCAGATACCA AACAGAAGAT TTACACTTTG GTGGTCTCCC
    ACAATCAACA GAGCCAACGT CTATGTTGGA TTCCAGGTGC AACTGGATTT GACTGGGATT
    TTCATGCATG GCAAGATTCC TACATTAAAA ATCTCATTGA TTCAGATATT CAGGGCCCAC
    TTGTGGCAAA AGATTCATGA AAGCATTGTC ATGGATTTAT GTCAGGTGTT TGATCAGGAG
    CTGGATGCTT TGGAGATTGA GACTGTGCAG AAAGAGACCA TCCACCCTAG GAAGTCCTAT
    AAGATGAACT CGTCCTGTGC TGATATTCTG CTCTTTGCCT CCTACAAATG GAATGTGTCT
    CGTCCCTCCT TGCTTGCTGA CTCAAAGGAT GTAATGGACA GCACAACCAC CCAGAAGTAC
    TGGATTGATA TACAGCTGAG ATGGGGTGAC TATGACTCCC ACGACATTGA AAGATATGCA
    CGTGCCAAAT TTTTGGATTA TACAACGGAT AACATGAGTA TCTATCCCTC ACCTACTGGT
    GTTCTTATTG CCATTGACCT TGCCTATAAC CTGCACAGTG CATATGGAAA CTGGTTCCCA
    GGGGGAAAGC CTCTAATACA GCAAGCAATG GCAAAGATAA TGAAGGCAAA CCCTGCCCTC
    TACGTGCTGC GGGAGCGCAT CCGTAAAGGA TTACAGCTTT ACTCTTCTGA GCCCACTGAG
    CCTTACCTGT CTTCTCAGAA CTATGGGGAA CTCTTCTCCA ATCAAATCAT CTGGTTTGTG
    GATGACACAA ATGTATACAG AGTCACCATC CACAAGACCT TTGAAGGTAA CCTGACAACC
    AAGCCAATCA ATGGAGCCAT TTTCATCTTT AATCCCAGGA CTGGCCAGCT CTTCTTAAAA
    ATTATTCATA CTTCTGTGTG GGCTGGACAG AAGAGATTGG GTCAGCTGGC CAAATGGAAA
    ACGGCAGAAG AAGTAGCTGC ATTGATTCGA TCTCTTCCAG TTGAGGAGCA GCCGAAACAA
    ATCATTGTCA CCAGGAAAGG CATGTTGGAT CCACTCGAGG TGCACTTGCT TGACTTCCCA
    AACATTGTCA TCAAAGGATC AGAATTACAG TTGCCTTTCC AGGCCTGTCT GAAGGTGGAG
    AAATTTGGTG ATCTGATCTT AAAGGCTACA GAGCCTCAGA TGGTTCTGTT CAATCTATAT
    GATGACTGGC TGAAGACGAT CTCTTCATAC ACGGCATTCT CTCGATTAAT TTTAATTCTA
    AGAGCCTTAC ATGTCAACAA TGACCGTGCA AAAGTGATCT TGAAACCTGA CAAGACCACT
    GTCACAGAGC CCCATCATAT CTGGCCCACA CTGACAGATG AGGAATGGAT TAAGGTTGAA
    GTTCAGCTAA AGGATTTGAT TTTAGCAGAT TATGGAAAGA AGAACAACGT AAATGTGGCC
    TCACTGACAC AGTCTGAAAT CAGAGACATC ATTCTGGGTA TGGAAATATC TGCTCCATCC
    CAGCAAAGGC AGCAGATTGC AGAAATTGAA AAGCAAACAA AAGAACAGTC TCAGCTCACT
    GCTACTCAGA CGCGCACTGT CAACAAACAC GGAGATGAGA TTATCACATC TACCACCAGC
    AACTACGAAA CACAGACGTT CTCTTCAAAG ACTGAGTGGC GAGTCAGAGC AATATCTGCT
    GCCAACCTTC ACCTCCGAAC CAATCACATC TATGTATCGT CTGATGACAT TAAGGAAACT
    GGATACACCT ACATATTGCC CAAAAATGTG CTTAAGAAAT TCATTTGCAT CTCTGATCTT
    AGAGCACAGA TTGCTGGTTA CCTGTATGGT GTGAGCCCCC CAGATAATCC CCAAGTGAAG
    GAAATCCGTT GCATTGTTAT GGTGCCCCAG TGGGGAACTC ATCAAACTGT CCACCTGCCA
    AGCCAGCTGC CCCAACATGA ATACCTTAAG GAAATGGAGC CATTAGGATG GGTTCACACT
    CAGCCAAATG AGTCCCCACA GTTGTCACCT CAAGATGTCA CCACCCATGC CAAAGTAATG
    GCTGACAATC CTGCCTGGGA TGGAGAGAAA ACCATAATCA TTACTTGCAG CTTTACTCCA
    GGTTCATGCA CGTTAACCGC TTATAAACTG ACTCCCAGTG GTTATGAATG GGGACGGCAA
    AACACAGATA AAGGAAACAA TCCCAAGGGC TACTTGCCAT CGCATTATGA GAGGGTCCAG
    ATGCTGCTGT CTGATCGCTT CCTTGGGTTC TTCATGGTGC CAGCACAAGC GTCCTGGAAC
    TACAACTTCA TGGGTGTGAG ACATGACCCA AACATGAAAT ATGAGTTACA GCTGGCCAAC
    CCGAAGGAGT TTTACCATGA AGTACACCGG CCATCGCATT TCCTAAACTT TGCTCTGCTG
    CAGGAGGGTG AGGTGTATTC GGCTGACCGG GAAGATCTGT ATGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004911723.1
    CDS105..7112
    Translation

    Target ORF information:

    RefSeq Version XM_004911666.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis pre-mRNA processing factor 8 (prpf8), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004911666.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCCGGGG TGTTTCCATA TAGACCACAG GCACCGCCAA TGCCCGGCCC CCCAAGTGGA 
    ACACCGGACT ACATGTCTGA AGAGAAGCTG CAGGAAAAAG CACGAAAATG GCAGCAGCTA
    CAGGCAAAAC GCTATTCAGA GAAAAGAAAA TTTGGGTTTG TGGATGCACA GAAAGAAGAT
    ATGCCTCCAG AACATGTTCG CAAAATTATT CGTGATCATG GAGACATGAC CAACAGAAAG
    TTCCGACATG ATAAGAGAGT TTACCTTGGA GCATTGAAGT ATATGCCACA TGCTGTCTTA
    AAGTTACTTG AAAACATGCC CATGCCTTGG GAGCAGATCC GTGATGTCCC TGTGCTGTAT
    CATATAACAG GAGCCATCTC ATTTGTTAAT GAGATTCCAT GGGTCATTGA GCCTGTCTAC
    ATAGCACAGT GGGGGTCAAT GTGGATTATG ATGAGGCGAG AAAAAAGAGA CAGACGACAC
    TTCAAGAGGA TGAGGTTTCC TCCATTTGAT GATGAGGAGC CTCCACTGGA TTATGCTGAC
    AATATCCTTG ATGTTGAACC CCTGGAAGCC ATTCAGCTTG AACTGGACCC AGAGGAAGAT
    GCTCCAGTCA CTGACTGGTT CTATGATCAC CAGCCCTTAA GAGACAACAG AAAGTATGTG
    AATGGTACTA CTTATCAGCG GTGGCAGTTT ACACTTCCGA TGATGTCTAC ATTATATCGA
    TTGGCTAATC AGCTTCTGAC TGACCTGGTT GATGATAACT ACTTCTACCT GTTTGACCTA
    AAGGCATTCT TTACCTCCAA AGCATTAAAC ATGGCTATTC CTGGAGGGCC AAAATTTGAG
    CCTCTTGTTC GTGACATTAA CCTGCAGGAT GAAGATTGGA ATGAGTTTAA TGATATTAAC
    AAGATAATTA TCCGACAGCC AATTCGAACA GAATACAAGA TTGCTTTTCC TTACCTGTAC
    AACAATCTTC CTCACCATGT ACATTTAACC TGGTATCACA CACCAAATGT GGTTTTTATC
    AAAACTGAGG ATCCTGATTT ACCAGCATTC TATTTTGACC CACTGATTAA TCCCATATCA
    CACAGGCATT CTGTAAAGAG CCAAGAGCCA TTGCCAGATG ATGATGAGGA ATTTGAATTG
    CCAGAATATG TTGAACCATT CCTTAAAGAC ACTCCTCTGT ATTCAGATAA CACAGCTAAT
    GGAATTGCTT TACTTTGGGC TCCTCGTCCT TTCAACTTGC GTTCTGGTAG GACCAGGAGG
    GCTATTGATA TTCCTCTTGT GAAAAACTGG TACAGAGAGC ATTGTCCTGC TGGCCAGCCA
    GTAAAAGTCA GAGTATCTTA CCAGAAATTG CTGAAGTACT ATGTGCTAAA TGCTTTGAAA
    CACAGGCCTC CAAAAGCTCA GAAGAAAAGG TATCTCTTCC GTTCATTTAA AGCCACAAAA
    TTCTTTCAGT CGACAAAGTT GGACTGGGTG GAAGTAGGCC TGCAGGTTTG CCGCCAAGGC
    TATAACATGC TGAACTTGCT GATTCACAGA AAGAACCTTA ACTATCTTCA TCTGGATTAC
    AATTTCAATT TGAAGCCAGT AAAAACGCTG ACCACAAAGG AACGCAAAAA GTCTCGGTTC
    GGCAATGCCT TCCATCTTTG CCGCGAGGTC CTGCGTCTAA CCAAACTAGT TGTGGACAGC
    CATGTGCAAT ACAGACTGGG GAATGTTGAT GCTTTCCAGT TGGCAGACGG ATTACAGTAT
    ATTTTTGCCC ATGTGGGGCA GCTGACTGGA ATGTACCGTT ACAAGTACAA ACTAATGAGA
    CAAATTCGCA TGTGCAAGGA CCTGAAGCAT CTGATCTACT ACAGGTTCAA TACAGGTCCA
    GTTGGAAAGG GGCCTGGCTG TGGATTTTGG GCAGCAGGCT GGAGAGTCTG GCTCTTTTTT
    ATGCGTGGTA TCACACCTTT GCTGGAAAGG TGGCTTGGAA ATCTGCTTGC TAGACAATTT
    GAAGGTCGCC ATTCCAAAGG AGTTGCGAAA ACTGTGACAA AACAGCGAGT GGAGTCTCAC
    TTTGACTTGG AGCTCAGAGC TGCTGTTATG CATGACATAT TGGACATGAT GCCAGAGGGA
    ATCAAGCAGA ACAAAGCCAG AACCATCTTG CAGCACTTGA GTGAAGCTTG GAGATGCTGG
    AAAGCTAACA TTCCCTGGAA GGTACCAGGT CTTCCAACAC CTATTGAAAA CATGATTCTG
    CGATATGTAA AGGCCAAAGC GGACTGGTGG ACAAATACTG CGCATTACAA CAGAGAACGT
    ATTAGACGTG GTGCCACTGT CGACAAAACT GTGTGTAAGA AGAATCTGGG CCGTCTGACT
    CGTTTGTACT TGAAAGCAGA ACAAGAGAGA CAGCACAACT ACCTGAAGGA TGGGCCTTAC
    ATTACTGCAG AGGAGGCAGT TGCTGTGTAC ACTACCACTG TCCACTGGCT AGAGAGTAGG
    AGGTTTTCTC CAATTCCTTT TCCTCCTCTT TCCTACAAAC ATGATACAAA ACTACTCATT
    CTAGCACTTG AGAGACTGAA GGAAGCATAC AGTGTGAAGT CGCGTTTGAA TCAGTCTCAG
    AGGGAAGAAT TGGGTTTAAT CGAACAGGCG TATGACAACC CTCACGAAGC TCTGTCCAGA
    ATCAAGCGTC ACTTGCTCAC CCAAAGAGCT TTCAAGGAGG TTGGAATAGA GTTTATGGAT
    CTCTACAGCC ACCTTGTTCC TGTTTATGAC GTGGAACCAC TGGAAAAAAT TACTGATGCC
    TACCTGGATC AGTACCTGTG GTATGAAGCA GATAAAAGAA GGCTTTTCCC TCCATGGATC
    AAGCCTGCAG ATACAGAGCC ACCTCCCTTG TTGGTCTACA AGTGGTGCCA AGGCATAAAT
    AACCTGCAGG ATGTTTGGGA CACCACAGAA GGTGAATGCA ATGTGATGCT GGAGTCACGC
    TTTGAAAAGA TGTATGAAAA GATTGACTTG ACTCTGCTGA ATCGTCTGCT GCGTCTAATT
    GTTGATCACA ATATTGCTGA TTACATGACT GCAAAAAACA ACGTTGTGAT AAATTACAAG
    GATATGAACC ACACAAATTC CTATGGCATT ATTCGTGGTC TCCAGTTTGC TTCGTTTATT
    GTCCAGTACT ATGGTCTGGT GATGGATCTT CTGGTTCTTG GTTTACACAG AGCTAGTGAA
    ATGGCTGGGC CACCACAAAT GCCAAATGAC TTTCTAAGTT TCCAAGATGT GGCAACAGAA
    TCGGCCCATC CTATCCGCCT GTTTTGTCGA TACATTGACC GCATTCACAT TTTTTTCAGA
    TTTTCAGCTG ATGAAGCCAG AGACCTTATT CAGAGATATT TAACAGAACA TCCAGATCCC
    AATAATGAGA ATATTGTGGG CTACAACAAT AAAAAGTGTT GGCCAAGGGA TGCCAGAATG
    CGCCTCATGA AACATGATGT AAATCTGGGC CGTGCAGTGT TTTGGGATAT AAAAAATAGA
    CTGCCTCGTT CTGTCACCAC TGTGCAGTGG GAGAACAGTT TTGTGTCTGT GTACAGTAAA
    GACAATCCAA ACCTGCTGTT TAATATGTGC GGCTTTGAAT GCCGCATACT TCCCAAATGC
    CGCACAAGCT ATGAAGAGTT TACGCACAAG GATGGTGTCT GGAACCTGCA AAATGAAGTG
    ACCAAAGAAC GCACAGCTCA GTGCTTCTTA AGAGTTGATG ACGAGTCCAT GCAGAGATTT
    CACAACAGAG TCCGTCAAAT TCTTATGGCT TCCGGGTCCA CAACCTTTAC AAAAATTGTG
    AACAAATGGA ATACAGCACT TATTGGCCTA ATGACTTATT TCCGTGAGGC TGTGGTCAAC
    ACCCAAGAAC TACTTGATCT GCTGGTGAAA TGTGAGAACA AAATCCAAAC AAGAATAAAG
    ATTGGGCTGA ACTCTAAAAT GCCAAGCAGG TTCCCGCCTG TTGTTTTTTA CACACCAAAG
    GAACTTGGAG GTTTGGGCAT GTTGTCCATG GGACATGTTT TAATTCCACA GTCTGACCTA
    AGGTGGTCTA AGCAGACAGA TGTTGGCATA ACCCATTTCC GTTCTGGCAT GAGTCATGAG
    GAGGATCAAC TGATTCCTAA TCTGTATCGT TACATTCAGC CTTGGGAAAG TGAGTTTATA
    GACTCTCAGA GAGTCTGGGC TGAGTATGCC TTAAAGAGGC AGGAGGCGAT TGCTCAAAAC
    AGACGCCTGA CGCTTGAAGA CTTAGAAGAC TCATGGGACA GGGGAATTCC ACGTATTAAT
    ACACTGTTTC AGAAAGATCG GCACACACTT GCTTATGACA AGGGTTGGAG AGTTCGCACA
    GACTTTAAAC AGTACCAGGT GCTGAAACAA AATCCGTTCT GGTGGACACA TCAGCGTCAT
    GATGGAAAGC TATGGAATCT GAACAACTAC CGCACAGATA TGATTCAGGC CCTTGGAGGG
    GTGGAGGGTA TACTGGAGCA CACGCTATTT AAAGGCACTT ACTTTCCCAC ATGGGAGGGT
    CTTTTCTGGG AAAAGGCCAG CGGCTTTGAA GAATCAATGA AATGGAAGAA ACTGACCAAT
    GCTCAGCGTT CTGGTTTAAA CCAGATACCA AACAGAAGAT TTACACTTTG GTGGTCTCCC
    ACAATCAACA GAGCCAACGT CTATGTTGGA TTCCAGGTGC AACTGGATTT GACTGGGATT
    TTCATGCATG GCAAGATTCC TACATTAAAA ATCTCATTGA TTCAGATATT CAGGGCCCAC
    TTGTGGCAAA AGATTCATGA AAGCATTGTC ATGGATTTAT GTCAGGTGTT TGATCAGGAG
    CTGGATGCTT TGGAGATTGA GACTGTGCAG AAAGAGACCA TCCACCCTAG GAAGTCCTAT
    AAGATGAACT CGTCCTGTGC TGATATTCTG CTCTTTGCCT CCTACAAATG GAATGTGTCT
    CGTCCCTCCT TGCTTGCTGA CTCAAAGGAT GTAATGGACA GCACAACCAC CCAGAAGTAC
    TGGATTGATA TACAGCTGAG ATGGGGTGAC TATGACTCCC ACGACATTGA AAGATATGCA
    CGTGCCAAAT TTTTGGATTA TACAACGGAT AACATGAGTA TCTATCCCTC ACCTACTGGT
    GTTCTTATTG CCATTGACCT TGCCTATAAC CTGCACAGTG CATATGGAAA CTGGTTCCCA
    GGGGGAAAGC CTCTAATACA GCAAGCAATG GCAAAGATAA TGAAGGCAAA CCCTGCCCTC
    TACGTGCTGC GGGAGCGCAT CCGTAAAGGA TTACAGCTTT ACTCTTCTGA GCCCACTGAG
    CCTTACCTGT CTTCTCAGAA CTATGGGGAA CTCTTCTCCA ATCAAATCAT CTGGTTTGTG
    GATGACACAA ATGTATACAG AGTCACCATC CACAAGACCT TTGAAGGTAA CCTGACAACC
    AAGCCAATCA ATGGAGCCAT TTTCATCTTT AATCCCAGGA CTGGCCAGCT CTTCTTAAAA
    ATTATTCATA CTTCTGTGTG GGCTGGACAG AAGAGATTGG GTCAGCTGGC CAAATGGAAA
    ACGGCAGAAG AAGTAGCTGC ATTGATTCGA TCTCTTCCAG TTGAGGAGCA GCCGAAACAA
    ATCATTGTCA CCAGGAAAGG CATGTTGGAT CCACTCGAGG TGCACTTGCT TGACTTCCCA
    AACATTGTCA TCAAAGGATC AGAATTACAG TTGCCTTTCC AGGCCTGTCT GAAGGTGGAG
    AAATTTGGTG ATCTGATCTT AAAGGCTACA GAGCCTCAGA TGGTTCTGTT CAATCTATAT
    GATGACTGGC TGAAGACGAT CTCTTCATAC ACGGCATTCT CTCGATTAAT TTTAATTCTA
    AGAGCCTTAC ATGTCAACAA TGACCGTGCA AAAGTGATCT TGAAACCTGA CAAGACCACT
    GTCACAGAGC CCCATCATAT CTGGCCCACA CTGACAGATG AGGAATGGAT TAAGGTTGAA
    GTTCAGCTAA AGGATTTGAT TTTAGCAGAT TATGGAAAGA AGAACAACGT AAATGTGGCC
    TCACTGACAC AGTCTGAAAT CAGAGACATC ATTCTGGGTA TGGAAATATC TGCTCCATCC
    CAGCAAAGGC AGCAGATTGC AGAAATTGAA AAGCAAACAA AAGAACAGTC TCAGCTCACT
    GCTACTCAGA CGCGCACTGT CAACAAACAC GGAGATGAGA TTATCACATC TACCACCAGC
    AACTACGAAA CACAGACGTT CTCTTCAAAG ACTGAGTGGC GAGTCAGAGC AATATCTGCT
    GCCAACCTTC ACCTCCGAAC CAATCACATC TATGTATCGT CTGATGACAT TAAGGAAACT
    GGATACACCT ACATATTGCC CAAAAATGTG CTTAAGAAAT TCATTTGCAT CTCTGATCTT
    AGAGCACAGA TTGCTGGTTA CCTGTATGGT GTGAGCCCCC CAGATAATCC CCAAGTGAAG
    GAAATCCGTT GCATTGTTAT GGTGCCCCAG TGGGGAACTC ATCAAACTGT CCACCTGCCA
    AGCCAGCTGC CCCAACATGA ATACCTTAAG GAAATGGAGC CATTAGGATG GGTTCACACT
    CAGCCAAATG AGTCCCCACA GTTGTCACCT CAAGATGTCA CCACCCATGC CAAAGTAATG
    GCTGACAATC CTGCCTGGGA TGGAGAGAAA ACCATAATCA TTACTTGCAG CTTTACTCCA
    GGTTCATGCA CGTTAACCGC TTATAAACTG ACTCCCAGTG GTTATGAATG GGGACGGCAA
    AACACAGATA AAGGAAACAA TCCCAAGGGC TACTTGCCAT CGCATTATGA GAGGGTCCAG
    ATGCTGCTGT CTGATCGCTT CCTTGGGTTC TTCATGGTGC CAGCACAAGC GTCCTGGAAC
    TACAACTTCA TGGGTGTGAG ACATGACCCA AACATGAAAT ATGAGTTACA GCTGGCCAAC
    CCGAAGGAGT TTTACCATGA AGTACACCGG CCATCGCATT TCCTAAACTT TGCTCTGCTG
    CAGGAGGGTG AGGTGTATTC GGCTGACCGG GAAGATCTGT ATGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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