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neu4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Summary

Gene Symbol neu4
Entrez Gene ID 100486976
Full Name neuraminidase 4 (sialidase)
General protein information
Preferred Names

neuraminidase 4 (sialidase)

Names

sialidase-4
sialidase NEU4
Gene Type protein-coding
Organism Xenopus tropicalis(tropical clawed frog)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_012970212.2 XP_012825666.1 sialidase-4 isoform X1
XM_012970212.3 XP_012825666.1 sialidase-4 isoform X1
NM_001278746.1 NP_001265675.1 sialidase-4

Pathways from BioSystems


Homologies


Bos taurus (cattle) NEU4 XP_002686672.1
Rattus norvegicus (Norway rat) Neu4 NP_001101704.1
Xenopus tropicalis (tropical clawed frog) LOC100486976 XP_002943103.2
NEU4 XP_001095081.2
Mus musculus (house mouse) Neu4 NP_776133.1
Danio rerio (zebrafish) neu4 NP_001018384.1
Homo sapiens (human) NEU4 NP_001161071.1
Gallus gallus (chicken) NEU4 XP_004937059.1

Gene Ontology


Bibliography

Related articles in PubMed

New insights on the sialidase protein family revealed by a phylogenetic analysis in metazoa.
Giacopuzzi E, Bresciani R, Schauer R, Monti E, Borsani G
PloS one7(8)e44193(2012)

Gallus gallus NEU3 sialidase as model to study protein evolution mechanism based on rapid evolving loops.
Giacopuzzi E, Barlati S, Preti A, Venerando B, Monti E, Borsani G, Bresciani R
BMC biochemistry1245(2011 Aug)

Molecular cloning and biochemical characterization of sialidases from zebrafish (Danio rerio).
Manzoni M, Colombi P, Papini N, Rubaga L, Tiso N, Preti A, Venerando B, Tettamanti G, Bresciani R, Argenton F, Borsani G, Monti E
The Biochemical journal408(3)395-406(2007 Dec)

Molecular cloning and characterization of NEU4, the fourth member of the human sialidase gene family.
Monti E, Bassi MT, Bresciani R, Civini S, Croci GL, Papini N, Riboni M, Zanchetti G, Ballabio A, Preti A, Tettamanti G, Venerando B, Borsani G
Genomics83(3)445-53(2004 Mar)

Identification and expression of NEU3, a novel human sialidase associated to the plasma membrane.
Monti E, Bassi MT, Papini N, Riboni M, Manzoni M, Venerando B, Croci G, Preti A, Ballabio A, Tettamanti G, Borsani G
The Biochemical journal349(Pt 1)343-51(2000 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following neu4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the neu4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa20371 NM_001278746.1
Latest version!		 Xenopus tropicalis neuraminidase 4 (sialidase) (neu4), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $258.30
$369.00
OXa33760 XM_012970212.3
Latest version!		 Xenopus tropicalis neuraminidase 4 (sialidase) (neu4), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $223.30
$319.00
View Old Accession Versions >>
OXa33760 XM_012970212.2 Xenopus tropicalis neuraminidase 4 (sialidase) (neu4), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $258.30
$369.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OXa20371
Clone ID Related Accession (Same CDS sequence) NM_001278746.1
Accession Version NM_001278746.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1452bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-21
Organism Xenopus tropicalis(tropical clawed frog)
Product sialidase-4
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BK008559.1. On or before Jul 15, 2013 this sequence version replaced XM_002943057.2, XM_004917832.1. ##Evidence-Data-START## Transcript exon combination :: BK008559.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028309, SAMD00028310 [ECO:0000350] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGGGTCCC GCCATGTCCC TGCACGCACA GTTCTTTTTG AGAAGGAGAA AAATGGAGTG 
ACCTATCGTG TGCCGGCCCT GCTGTACCTC CCCCGCTGGC GCACCCTGTT GGCCTTTGCA 
GAGGAGAGGC TCAGTGCAGA TGATGCCCAT GCTAACCTCA TCGTCCTTCG CCGTGGAATA 
TTCTACCGCA ACTATGTGGA GTGGGAGGAC ATGCACGCTG TGGAACAAGC TTCTTTGCCC 
GGCCATCGTT CTATGAATCC TTGTCCAGTC TATGATGATG CCACTGGGCA ATTGTTCCTC 
TTCTTCATCG CAGTCTTTGG CAGGACCACA GAATCCTATC AGATTATTAC TGGGCAGAAT 
GCGGCCAGGC TTTGCTATGT GAGAAGTTTA GACCAGGGAA AGAGTTGGAG TGAAGTCACA 
GACCTAACCC ACCAGGTCAT TGGGGAATCA CTGAAAGGTG AGTGGGCCAC ATTTGCCTTG 
GGACCAGGTC ATGGAATTCA GCTGAGAAAT GGGCGATTAA TTATTCCTGC TTATACATAT 
CACATTGAGC AGAGACATTG CTTTGGGAGC TTCTGCCAGA CCACTGCCCG CGCTTTTGCA 
TTCCATAGTG ATGACCATGG GCAAAGCTGG AATTTCGGAG ACCTCATACC AAATTTACAC 
AGCGTGGAGT GCCAGATGGT ATCAGTGGAT GAGGCTGATG GTAACAATAT CCTGTACTGC 
AATGCCCGAA GCTCCCTTGG ATTCAGAGTA CAAGCTCTGA GCACTGATGA TGGTGTTGTA 
TTTGAGCCAG GTCAGCTGAT TGAGAAGTTG GTTGAGACCC CCACAGGGTG TCATGGAAGT 
GTCATTGGTT TTCCAGCTCC GCTGCACACT CTGTATAGTC CATGCCCTGA CCATCATTTA 
ACCATGCAAC TAAAGTGCCA AAAAGCCCAT AATGATGTAT CAGGAAAAAA TGATCAGCAA 
AAGATAAAGG ATGAAGCTAA ACATATAAAT CAGTTCAGCA GAAAGATGCT TCAATGGAAA 
TGGAAAACAG AAGACCATCG AGGAATGAAG GAAACATTCT ACAAGAATAT GCCAGATGTC 
CGAAATCCAA CATGGGTGCT CTACTCCCAT CCAACAAGTG CCAGATCACG TGTGAACCTT 
GGTGTCTATC TCACCTCATA TCCCAGAGAT GCAGACAGCT GGACAGGTCC CTGGGTAATC 
TACGAGGGTC CCAGTGCCTA TTCAGACCTT GCATATCTCG GGCCACCATC GGGTGAAAAA 
AGTGCAATGG GGTCCTTGCC AGTTGTTGTC TTTGCTTGTC TTTATGAAAA TGGTTTGAGT 
TCCCCATATG AACAGATTTC ATTCAGCATC TTCACCCTAT ATGAAGTCAT CCAGAATTTA 
ACTCCCAGCT CAGAAGTTAT GCGCCAGACC AAGATGAAGC ACCGAATAAA GAAGAACTGC 
ACAACATCTT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001265675.1
CDS1..1452
Translation

Target ORF information:

RefSeq Version NM_001278746.1
Organism Xenopus tropicalis(tropical clawed frog)
Definition Xenopus tropicalis neuraminidase 4 (sialidase) (neu4), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001278746.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGGGTCCC GCCATGTCCC TGCACGCACA GTTCTTTTTG AGAAGGAGAA AAATGGAGTG 
ACCTATCGTG TGCCGGCCCT GCTGTACCTC CCCCGCTGGC GCACCCTGTT GGCCTTTGCA 
GAGGAGAGGC TCAGTGCAGA TGATGCCCAT GCTAACCTCA TCGTCCTTCG CCGTGGAATA 
TTCTACCGCA ACTATGTGGA GTGGGAGGAC ATGCACGCTG TGGAACAAGC TTCTTTGCCC 
GGCCATCGTT CTATGAATCC TTGTCCAGTC TATGATGATG CCACTGGGCA ATTGTTCCTC 
TTCTTCATCG CAGTCTTTGG CAGGACCACA GAATCCTATC AGATTATTAC TGGGCAGAAT 
GCGGCCAGGC TTTGCTATGT GAGAAGTTTA GACCAGGGAA AGAGTTGGAG TGAAGTCACA 
GACCTAACCC ACCAGGTCAT TGGGGAATCA CTGAAAGGTG AGTGGGCCAC ATTTGCCTTG 
GGACCAGGTC ATGGAATTCA GCTGAGAAAT GGGCGATTAA TTATTCCTGC TTATACATAT 
CACATTGAGC AGAGACATTG CTTTGGGAGC TTCTGCCAGA CCACTGCCCG CGCTTTTGCA 
TTCCATAGTG ATGACCATGG GCAAAGCTGG AATTTCGGAG ACCTCATACC AAATTTACAC 
AGCGTGGAGT GCCAGATGGT ATCAGTGGAT GAGGCTGATG GTAACAATAT CCTGTACTGC 
AATGCCCGAA GCTCCCTTGG ATTCAGAGTA CAAGCTCTGA GCACTGATGA TGGTGTTGTA 
TTTGAGCCAG GTCAGCTGAT TGAGAAGTTG GTTGAGACCC CCACAGGGTG TCATGGAAGT 
GTCATTGGTT TTCCAGCTCC GCTGCACACT CTGTATAGTC CATGCCCTGA CCATCATTTA 
ACCATGCAAC TAAAGTGCCA AAAAGCCCAT AATGATGTAT CAGGAAAAAA TGATCAGCAA 
AAGATAAAGG ATGAAGCTAA ACATATAAAT CAGTTCAGCA GAAAGATGCT TCAATGGAAA 
TGGAAAACAG AAGACCATCG AGGAATGAAG GAAACATTCT ACAAGAATAT GCCAGATGTC 
CGAAATCCAA CATGGGTGCT CTACTCCCAT CCAACAAGTG CCAGATCACG TGTGAACCTT 
GGTGTCTATC TCACCTCATA TCCCAGAGAT GCAGACAGCT GGACAGGTCC CTGGGTAATC 
TACGAGGGTC CCAGTGCCTA TTCAGACCTT GCATATCTCG GGCCACCATC GGGTGAAAAA 
AGTGCAATGG GGTCCTTGCC AGTTGTTGTC TTTGCTTGTC TTTATGAAAA TGGTTTGAGT 
TCCCCATATG AACAGATTTC ATTCAGCATC TTCACCCTAT ATGAAGTCAT CCAGAATTTA 
ACTCCCAGCT CAGAAGTTAT GCGCCAGACC AAGATGAAGC ACCGAATAAA GAAGAACTGC 
ACAACATCTT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OXa33760
Clone ID Related Accession (Same CDS sequence) XM_012970212.2 , XM_012970212.3
Accession Version XM_012970212.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1449bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-09-07
Organism Xenopus tropicalis(tropical clawed frog)
Product sialidase-4 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970212.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGGGTCCC GCCATGTCCC TGCACGCACA GTTCTTTTTG AGAAGGAGAA AAATGGAGTG 
ACCTATCGTG TGCCGGCCCT GCTGTACCTC CCCCGCTGGC GCACCCTGTT GGCCTTTGCA 
GAGGAGAGGC TCAGTGCAGA TGATGCCCAT GCTAACCTCA TCGTCCTTCG CCGTGGAATA 
TTCTACCGCA ACTATGTGGA GTGGGAGGAC ATGCACGCTG TGGAACAAGC TTCTTTGCCC 
GGCCATCGTT CTATGAATCC TTGTCCAGTC TATGATGATG CCACTGGGCA ATTGTTCCTC 
TTCTTCATCG CAGTCTTTGG CAGGACCACA GAATCCTATC AGATTATTAC TGGGCAGAAT 
GCGGCCAGGC TTTGCTATGT GAGAAGTTTA GACCAGGGAA AGAGTTGGAG TGAAGTCACA 
GACCTAACCC ACCAGGTCAT TGGGGAATCA CTGAAAGAGT GGGCCACATT TGCCTTGGGA 
CCAGGTCATG GAATTCAGCT GAGAAATGGG CGATTAATTA TTCCTGCTTA TACATATCAC 
ATTGAGCAGA GACATTGCTT TGGGAGCTTC TGCCAGACCA CTGCCCGCGC TTTTGCATTC 
CATAGTGATG ACCATGGGCA AAGCTGGAAT TTCGGAGACC TCATACCAAA TTTACACAGC 
GTGGAGTGCC AGATGGTATC AGTGGATGAG GCTGATGGTA ACAATATCCT GTACTGCAAT 
GCCCGAAGCT CCCTTGGATT CAGAGTACAA GCTCTGAGCA CTGATGATGG TGTTGTATTT 
GAGCCAGGTC AGCTGATTGA GAAGTTGGTT GAGACCCCCA CAGGGTGTCA TGGAAGTGTC 
ATTGGTTTTC CAGCTCCGCT GCACACTCTG TATAGTCCAT GCCCTGACCA TCATTTAACC 
ATGCAACTAA AGTGCCAAAA AGCCCATAAT GATGTATCAG GAAAAAATGA TCAGCAAAAG 
ATAAAGGATG AAGCTAAACA TATAAATCAG TTCAGCAGAA AGATGCTTCA ATGGAAATGG 
AAAACAGAAG ACCATCGAGG AATGAAGGAA ACATTCTACA AGAATATGCC AGATGTCCGA 
AATCCAACAT GGGTGCTCTA CTCCCATCCA ACAAGTGCCA GATCACGTGT GAACCTTGGT 
GTCTATCTCA CCTCATATCC CAGAGATGCA GACAGCTGGA CAGGTCCCTG GGTAATCTAC 
GAGGGTCCCA GTGCCTATTC AGACCTTGCA TATCTCGGGC CACCATCGGG TGAAAAAAGT 
GCAATGGGGT CCTTGCCAGT TGTTGTCTTT GCTTGTCTTT ATGAAAATGG TTTGAGTTCC 
CCATATGAAC AGATTTCATT CAGCATCTTC ACCCTATATG AAGTCATCCA GAATTTAACT 
CCCAGCTCAG AAGTTATGCG CCAGACCAAG ATGAAGCACC GAATAAAGAA GAACTGCACA 
ACATCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_012825666.1
CDS144..1592
Translation

Target ORF information:

RefSeq Version XM_012970212.2
Organism Xenopus tropicalis(tropical clawed frog)
Definition Xenopus tropicalis neuraminidase 4 (sialidase) (neu4), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_012970212.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGGGTCCC GCCATGTCCC TGCACGCACA GTTCTTTTTG AGAAGGAGAA AAATGGAGTG 
ACCTATCGTG TGCCGGCCCT GCTGTACCTC CCCCGCTGGC GCACCCTGTT GGCCTTTGCA 
GAGGAGAGGC TCAGTGCAGA TGATGCCCAT GCTAACCTCA TCGTCCTTCG CCGTGGAATA 
TTCTACCGCA ACTATGTGGA GTGGGAGGAC ATGCACGCTG TGGAACAAGC TTCTTTGCCC 
GGCCATCGTT CTATGAATCC TTGTCCAGTC TATGATGATG CCACTGGGCA ATTGTTCCTC 
TTCTTCATCG CAGTCTTTGG CAGGACCACA GAATCCTATC AGATTATTAC TGGGCAGAAT 
GCGGCCAGGC TTTGCTATGT GAGAAGTTTA GACCAGGGAA AGAGTTGGAG TGAAGTCACA 
GACCTAACCC ACCAGGTCAT TGGGGAATCA CTGAAAGAGT GGGCCACATT TGCCTTGGGA 
CCAGGTCATG GAATTCAGCT GAGAAATGGG CGATTAATTA TTCCTGCTTA TACATATCAC 
ATTGAGCAGA GACATTGCTT TGGGAGCTTC TGCCAGACCA CTGCCCGCGC TTTTGCATTC 
CATAGTGATG ACCATGGGCA AAGCTGGAAT TTCGGAGACC TCATACCAAA TTTACACAGC 
GTGGAGTGCC AGATGGTATC AGTGGATGAG GCTGATGGTA ACAATATCCT GTACTGCAAT 
GCCCGAAGCT CCCTTGGATT CAGAGTACAA GCTCTGAGCA CTGATGATGG TGTTGTATTT 
GAGCCAGGTC AGCTGATTGA GAAGTTGGTT GAGACCCCCA CAGGGTGTCA TGGAAGTGTC 
ATTGGTTTTC CAGCTCCGCT GCACACTCTG TATAGTCCAT GCCCTGACCA TCATTTAACC 
ATGCAACTAA AGTGCCAAAA AGCCCATAAT GATGTATCAG GAAAAAATGA TCAGCAAAAG 
ATAAAGGATG AAGCTAAACA TATAAATCAG TTCAGCAGAA AGATGCTTCA ATGGAAATGG 
AAAACAGAAG ACCATCGAGG AATGAAGGAA ACATTCTACA AGAATATGCC AGATGTCCGA 
AATCCAACAT GGGTGCTCTA CTCCCATCCA ACAAGTGCCA GATCACGTGT GAACCTTGGT 
GTCTATCTCA CCTCATATCC CAGAGATGCA GACAGCTGGA CAGGTCCCTG GGTAATCTAC 
GAGGGTCCCA GTGCCTATTC AGACCTTGCA TATCTCGGGC CACCATCGGG TGAAAAAAGT 
GCAATGGGGT CCTTGCCAGT TGTTGTCTTT GCTTGTCTTT ATGAAAATGG TTTGAGTTCC 
CCATATGAAC AGATTTCATT CAGCATCTTC ACCCTATATG AAGTCATCCA GAATTTAACT 
CCCAGCTCAG AAGTTATGCG CCAGACCAAG ATGAAGCACC GAATAAAGAA GAACTGCACA 
ACATCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OXa33760
Clone ID Related Accession (Same CDS sequence) XM_012970212.2 , XM_012970212.3
Accession Version XM_012970212.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1449bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-17
Organism Xenopus tropicalis(tropical clawed frog)
Product sialidase-4 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970212.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGGGTCCC GCCATGTCCC TGCACGCACA GTTCTTTTTG AGAAGGAGAA AAATGGAGTG 
ACCTATCGTG TGCCGGCCCT GCTGTACCTC CCCCGCTGGC GCACCCTGTT GGCCTTTGCA 
GAGGAGAGGC TCAGTGCAGA TGATGCCCAT GCTAACCTCA TCGTCCTTCG CCGTGGAATA 
TTCTACCGCA ACTATGTGGA GTGGGAGGAC ATGCACGCTG TGGAACAAGC TTCTTTGCCC 
GGCCATCGTT CTATGAATCC TTGTCCAGTC TATGATGATG CCACTGGGCA ATTGTTCCTC 
TTCTTCATCG CAGTCTTTGG CAGGACCACA GAATCCTATC AGATTATTAC TGGGCAGAAT 
GCGGCCAGGC TTTGCTATGT GAGAAGTTTA GACCAGGGAA AGAGTTGGAG TGAAGTCACA 
GACCTAACCC ACCAGGTCAT TGGGGAATCA CTGAAAGAGT GGGCCACATT TGCCTTGGGA 
CCAGGTCATG GAATTCAGCT GAGAAATGGG CGATTAATTA TTCCTGCTTA TACATATCAC 
ATTGAGCAGA GACATTGCTT TGGGAGCTTC TGCCAGACCA CTGCCCGCGC TTTTGCATTC 
CATAGTGATG ACCATGGGCA AAGCTGGAAT TTCGGAGACC TCATACCAAA TTTACACAGC 
GTGGAGTGCC AGATGGTATC AGTGGATGAG GCTGATGGTA ACAATATCCT GTACTGCAAT 
GCCCGAAGCT CCCTTGGATT CAGAGTACAA GCTCTGAGCA CTGATGATGG TGTTGTATTT 
GAGCCAGGTC AGCTGATTGA GAAGTTGGTT GAGACCCCCA CAGGGTGTCA TGGAAGTGTC 
ATTGGTTTTC CAGCTCCGCT GCACACTCTG TATAGTCCAT GCCCTGACCA TCATTTAACC 
ATGCAACTAA AGTGCCAAAA AGCCCATAAT GATGTATCAG GAAAAAATGA TCAGCAAAAG 
ATAAAGGATG AAGCTAAACA TATAAATCAG TTCAGCAGAA AGATGCTTCA ATGGAAATGG 
AAAACAGAAG ACCATCGAGG AATGAAGGAA ACATTCTACA AGAATATGCC AGATGTCCGA 
AATCCAACAT GGGTGCTCTA CTCCCATCCA ACAAGTGCCA GATCACGTGT GAACCTTGGT 
GTCTATCTCA CCTCATATCC CAGAGATGCA GACAGCTGGA CAGGTCCCTG GGTAATCTAC 
GAGGGTCCCA GTGCCTATTC AGACCTTGCA TATCTCGGGC CACCATCGGG TGAAAAAAGT 
GCAATGGGGT CCTTGCCAGT TGTTGTCTTT GCTTGTCTTT ATGAAAATGG TTTGAGTTCC 
CCATATGAAC AGATTTCATT CAGCATCTTC ACCCTATATG AAGTCATCCA GAATTTAACT 
CCCAGCTCAG AAGTTATGCG CCAGACCAAG ATGAAGCACC GAATAAAGAA GAACTGCACA 
ACATCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_012825666.1
CDS158..1606
Translation

Target ORF information:

RefSeq Version XM_012970212.3
Organism Xenopus tropicalis(tropical clawed frog)
Definition Xenopus tropicalis neuraminidase 4 (sialidase) (neu4), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_012970212.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGGGTCCC GCCATGTCCC TGCACGCACA GTTCTTTTTG AGAAGGAGAA AAATGGAGTG 
ACCTATCGTG TGCCGGCCCT GCTGTACCTC CCCCGCTGGC GCACCCTGTT GGCCTTTGCA 
GAGGAGAGGC TCAGTGCAGA TGATGCCCAT GCTAACCTCA TCGTCCTTCG CCGTGGAATA 
TTCTACCGCA ACTATGTGGA GTGGGAGGAC ATGCACGCTG TGGAACAAGC TTCTTTGCCC 
GGCCATCGTT CTATGAATCC TTGTCCAGTC TATGATGATG CCACTGGGCA ATTGTTCCTC 
TTCTTCATCG CAGTCTTTGG CAGGACCACA GAATCCTATC AGATTATTAC TGGGCAGAAT 
GCGGCCAGGC TTTGCTATGT GAGAAGTTTA GACCAGGGAA AGAGTTGGAG TGAAGTCACA 
GACCTAACCC ACCAGGTCAT TGGGGAATCA CTGAAAGAGT GGGCCACATT TGCCTTGGGA 
CCAGGTCATG GAATTCAGCT GAGAAATGGG CGATTAATTA TTCCTGCTTA TACATATCAC 
ATTGAGCAGA GACATTGCTT TGGGAGCTTC TGCCAGACCA CTGCCCGCGC TTTTGCATTC 
CATAGTGATG ACCATGGGCA AAGCTGGAAT TTCGGAGACC TCATACCAAA TTTACACAGC 
GTGGAGTGCC AGATGGTATC AGTGGATGAG GCTGATGGTA ACAATATCCT GTACTGCAAT 
GCCCGAAGCT CCCTTGGATT CAGAGTACAA GCTCTGAGCA CTGATGATGG TGTTGTATTT 
GAGCCAGGTC AGCTGATTGA GAAGTTGGTT GAGACCCCCA CAGGGTGTCA TGGAAGTGTC 
ATTGGTTTTC CAGCTCCGCT GCACACTCTG TATAGTCCAT GCCCTGACCA TCATTTAACC 
ATGCAACTAA AGTGCCAAAA AGCCCATAAT GATGTATCAG GAAAAAATGA TCAGCAAAAG 
ATAAAGGATG AAGCTAAACA TATAAATCAG TTCAGCAGAA AGATGCTTCA ATGGAAATGG 
AAAACAGAAG ACCATCGAGG AATGAAGGAA ACATTCTACA AGAATATGCC AGATGTCCGA 
AATCCAACAT GGGTGCTCTA CTCCCATCCA ACAAGTGCCA GATCACGTGT GAACCTTGGT 
GTCTATCTCA CCTCATATCC CAGAGATGCA GACAGCTGGA CAGGTCCCTG GGTAATCTAC 
GAGGGTCCCA GTGCCTATTC AGACCTTGCA TATCTCGGGC CACCATCGGG TGAAAAAAGT 
GCAATGGGGT CCTTGCCAGT TGTTGTCTTT GCTTGTCTTT ATGAAAATGG TTTGAGTTCC 
CCATATGAAC AGATTTCATT CAGCATCTTC ACCCTATATG AAGTCATCCA GAATTTAACT 
CCCAGCTCAG AAGTTATGCG CCAGACCAAG ATGAAGCACC GAATAAAGAA GAACTGCACA 
ACATCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

New insights on the sialidase protein family revealed by a phylogenetic analysis in metazoa.
PloS one7(8)e44193(2012)
Giacopuzzi E,Bresciani R,Schauer R,Monti E,Borsani G


book

Gallus gallus NEU3 sialidase as model to study protein evolution mechanism based on rapid evolving loops.
BMC biochemistry1245(2011 Aug)
Giacopuzzi E,Barlati S,Preti A,Venerando B,Monti E,Borsani G,Bresciani R


book

Molecular cloning and biochemical characterization of sialidases from zebrafish (Danio rerio).
The Biochemical journal408(3)395-406(2007 Dec)
Manzoni M,Colombi P,Papini N,Rubaga L,Tiso N,Preti A,Venerando B,Tettamanti G,Bresciani R,Argenton F,Borsani G,Monti E


book

Molecular cloning and characterization of NEU4, the fourth member of the human sialidase gene family.
Genomics83(3)445-53(2004 Mar)
Monti E,Bassi MT,Bresciani R,Civini S,Croci GL,Papini N,Riboni M,Zanchetti G,Ballabio A,Preti A,Tettamanti G,Venerando B,Borsani G


book

Identification and expression of NEU3, a novel human sialidase associated to the plasma membrane.
The Biochemical journal349(Pt 1)343-51(2000 Jul)
Monti E,Bassi MT,Papini N,Riboni M,Manzoni M,Venerando B,Croci G,Preti A,Ballabio A,Tettamanti G,Borsani G