loxhd1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	loxhd1
Entrez Gene ID	100487617
Full Name	lipoxygenase homology domains 1
Synonyms	dfnb77,lh2d1
General protein information	Preferred Names lipoxygenase homology domains 1 Names lipoxygenase homology domain-containing protein 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018090855.1	XP_017946344.1	lipoxygenase homology domain-containing protein 1
XM_018090855.2	XP_017946344.1	lipoxygenase homology domain-containing protein 1 isoform X2
XM_031894937.1	XP_031750797.1	lipoxygenase homology domain-containing protein 1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LOXHD1	XP_001146229.1
Danio rerio (zebrafish)	loxhd1b	XP_001920640.3
Xenopus tropicalis (tropical clawed frog)	loxhd1	XP_002936557.2
	LOC702221	XP_001090494.2
Canis lupus familiaris (dog)	LOXHD1	XP_547589.3
Gallus gallus (chicken)	LOXHD1	XP_425221.4
Homo sapiens (human)	LOXHD1	NP_653213.6
Rattus norvegicus (Norway rat)	Loxhd1	NP_001099602.2
Bos taurus (cattle)	LOXHD1	XP_003587840.2
Danio rerio (zebrafish)	loxhd1a	XP_001920748.4

Gene Ontology

The following loxhd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the loxhd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa42263	XM_018090855.1 Latest version!	Xenopus tropicalis lipoxygenase homology domains 1 (loxhd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OXa49272	XM_018090855.2 Latest version!	Xenopus tropicalis lipoxygenase homology domains 1 (loxhd1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa49271	XM_031894937.1 Latest version!	Xenopus tropicalis lipoxygenase homology domains 1 (loxhd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

loxhd1 ( XM_018090855.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017946344.1 Xenopus tropicalis lipoxygenase homology domains 1 (loxhd1), mRNA.

Price & Availability↑

CloneID	OXa42263
Clone ID Related Accession (Same CDS sequence)	XM_018090855.1
Accession Version	XM_018090855.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	lipoxygenase homology domain-containing protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAG AAGACGAAGG GCAGGACCAG TCTCAAGCCA CACTGGGTTT GGAACAGAAA 
GCCAAATCGA CAACTTACAC AGTGACAATA AAGACCGGAG ACAAGAAGAA TTCTGGGACA 
GATGCCAATG TATTCTTAAT CTTGTATGGA GAGAAAGATG ATACAGGGTT CATGAACCTC 
AAGGCTTCCA AGACCAACAA GAATAAGTTT GAGCGAGGCC AAACTGATGT GTTTACCGTG 
GAGGCCGTGG ATATCGGGAC TCTCGCCAAA CTAAAAATTG GGCACGATAA CAAAGGCACC 
TCGGCTGGTT GGTTCCTGGA CTGGGTAGAG ATTGATGCAC CATCACTTGG TCAGCGGGTT 
AAGTTCCCCT GTGGTCGCTG GTTGGATAAA AGTGAAGATG ATGGGACCAT CGAAAGATAT 
CTGTTCCCAT CTGAACTGCA AACTGAGCAG TATGTTCCAT TTGTTCCGTA CGAGATCACA 
GTCTACACCA GCGATATATT CGCGGCCGGC ACGGACGCCG ACGTCTTTAT CGTTCTGTAT 
GGCCGAGACG GAGTCTGTAC CCAGCAGAAA TCCCTGTGTG TGAACAAGAG AGAGCGGAGA 
TTGTACTTTG AGCGAAACTC GGTGGATCAG TTCATTGTAG AGCTAGAAGA CGTTGGAGAT 
ATCATTGAGA AGATTCGCAT TGGGCATACT GACAAAGGCA TGAGCGCAGG GTGGCATTTA 
GACAGAGTGG AAATTCGTAG ACTGCTGCCA AATGGAAAGG GCTCCCAGAC CGTAACCTTT 
CCGTGTGAGC GGTGGCTTGC CAAATCCGAG GATGACGGAG AGACAGTGAG AGAACTAGTG 
CCATCCGATA TCTTTACTGA GAAACTCATG AAAGATGGGA CCTTGAAACA GATTGAGATG 
GAAGTAGAAG ACCCTCTGGA AACCCACACG TACAGCATAT CTGTGTTCAC CGGCGACATC 
TATGGTTCGG GTACCGACGC CAACGTCTTC ATCACCATTT ACGGAGATTT GGGAGACACG 
GGAGAGAGAA AACTCAGCAA GTCCGAGACC AACTCCAACA AGTTTGAGAG GGGACAGGTG 
GATAAATTCA ATATAGAAGC GGTGGATCTC GGCACCATCT ATAAGATTAG GATCCGGCAC 
GATAACTCTA TGTTGAACCC TGACTGGTAC CTGGAGAGGG TAGAGATCCT TAATGAAGTC 
ACGGAGGAGA CGTATCTGTT CATCTGTGAG CGATGGCTCT CCACCAAGAA GGAAGACAAG 
AAGATTGAAC GAATATTGTA TGAGAAGGAG TATGACGGGG AGCGACTCAG CTTGGGCAGC 
TCATCCAAAA GTTTCTGGGG ATCATCCCTC AGCCTGAGAA GTCAGGATAA CAATGCCAAT 
GTAAAGAGCA GGAACAGCCT AGATGGCTCC CAGCAAGATG GAACATTAAT CCCGTATCAC 
ATCACTATCA CCACCGGCCT TGACCGAGTT GGTGGGACAG AAAGTCGTGC TTATGTCATT 
ATAATGGGAC CTCAGAAGAT AAGGACAGAG CGACTTTGGC TGGACCTGCC CAAAGATAAG 
AAAGGCTTTG CGCCAGGGTC CGTGGAGAAG TTCTCTGTGG TGGGACTGGA TGTCGGAGAG 
ATTAAAAAGA TGGAGTTGGG CCATGATGGG GTTACCCCAG AAAGCTGCTG GCTAGTGGAT 
GAGATCCAAC TAACAGTGCC TAACAAAGGA GTCATGTATA CCTTTGTGTG TAAATGCTGG 
TTTGCCAAAG ATAGAGGAGA CGGTCTAACT GCCCGGGTCT TCAATATGCT GGATGCAACT 
GCCATCACTA TTGGCCATAA GATTCTCTAT GAGGTTACAG TTGTCACAGG AGATGTACCA 
AATGGGGGTA CTGATGCCAA TATTTATATT ACCTGCTTTG GAGCAAGTGG GAGCTCAGAG 
GAAATGCAGC TGCCAAAAGG GGATGACAGG TTTGAGAGAG GCCAGCGTGA CACTTTTGTC 
ATGGAGATCG CTGACATTGC CCCACTGAAG AAGATGAGAT TGCGTATCGA TGGGAAGGGC 
AGCCGCCCTG AGTGGTATTT CGAACAGATC TCCTTGAAGA ACTTGACCAC CGAAGAGGAG 
ACAGTGTTCA TGTATGGGGA CTGGATGTCC AAGACCAAGG GGGAGAGGAA GTCGCTCTCC 
TGTGAAATGG CGGCCACAGT AGACGGCGAA GAAATGGTGG AAACTACAAC GTACATTATA 
CAAGTCAAAA CCAGCGACGT GTCAGGAGCC GGTACCGACG CCAACGTGTT CCTGATCATC 
TTTGGAGAGA ACGGAGACAC CGGCACTCTG GCGCTGAAGC AGTCCAACAA TTCCAACAAG 
TTCGAAAGGA ATCAGAGTGA TGTCTTCAGA TTCCCTGACA TGTTGAGCCT CGGAGACCTG 
TGCAAAGTCC GGGTCTGGCA CGATAACTCA GGTATTGGCC CCGGCTGGCA CCTGGAATTT 
ATCGACATAA AGGACGAGAC CATGAGCAAG AGTTTCCGCT TCCAGTGCGA CCGATGGCTG 
GCCAAGAACG CCGATGACCA TCAGATCATC AGGGAACTCG CCTGTGCCAA CAACGATATC 
CTGGATCTGA AAGAGAAAAC CTCTTATGAG ATTGTGACGG TGACAAGCGA CAGGGATGAT 
GCAGAGACCA AGGACAACGT CTGGATCGTC TTGGAGGGCA AAAAAGGGAG GTCAAAAGAA 
TTTCTAATGG AGAACACTTC CAAAAAGCGG AGGTTTGCTC GGGATGCTAC AGATACATTC 
AAGTTTTCCT CCAAGAACCT TGGTGACATC GCTGCCATCT GCGTTGGCCA TTGCCCTAAG 
GACGGAAGGA AGTTTTCTCC GAAGCCGGAT TTATACTGGC ACGTCAAGGA GATCATCGTG 
ACCGAGACCG AACTCGGAAA CAAGTATGTG TTTAACTGCA ACGCCAAGGT GCCGCTGCGC 
AGCAAGCGAG ACGACTACAG AGTGTTCGAG TGTGCCAAGG CCATCGAGAG CTTCGCCAGC 
CGCGCCCGCA GTCTGGTGCC CGTCAAATAC GAAGTCATCG TGGTCACCGG GTTCGAAAAG 
GGGGCGGGGA CGGACGCCAA CGTGACCCTC GTCATGTACG GCTCCAACGG GGACTCCGGG 
AAGCACGCGC TGAAGCAGAA GATGCGCAAC CTCTTCGAGC GCGGGAAAAC CAACCGCTTC 
TACATAGAGA CCCTCGACCT GGGGGAGATG AAGAAAGTGC GGGTGGAGCA CGATAACAGC 
GGCCTGAGCC CCGGCTGGCT ACTCGAGCGG GTGGAGGTGA CCAACAGCGC CACCGGCGTC 
ACCACCATTT TCCCCTGCGG CAAGTGGCTG GACAAGAAAC GGGGCGACGG CGAGACCTGG 
AGGGAACTCT ACCCCAAATA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017946344.1
CDS	155..3538
Translation	MKKEDEGQDQSQATLGLEQKAKSTTYTVTIKTGDKKNSGTDANVFLILYGEKDDTGFMNLKASKTNKNKFERGQTDVFTVEAVDIGTLAKLKIGHDNKGTSAGWFLDWVEIDAPSLGQRVKFPCGRWLDKSEDDGTIERYLFPSELQTEQYVPFVPYEITVYTSDIFAAGTDADVFIVLYGRDGVCTQQKSLCVNKRERRLYFERNSVDQFIVELEDVGDIIEKIRIGHTDKGMSAGWHLDRVEIRRLLPNGKGSQTVTFPCERWLAKSEDDGETVRELVPSDIFTEKLMKDGTLKQIEMEVEDPLETHTYSISVFTGDIYGSGTDANVFITIYGDLGDTGERKLSKSETNSNKFERGQVDKFNIEAVDLGTIYKIRIRHDNSMLNPDWYLERVEILNEVTEETYLFICERWLSTKKEDKKIERILYEKEYDGERLSLGSSSKSFWGSSLSLRSQDNNANVKSRNSLDGSQQDGTLIPYHITITTGLDRVGGTESRAYVIIMGPQKIRTERLWLDLPKDKKGFAPGSVEKFSVVGLDVGEIKKMELGHDGVTPESCWLVDEIQLTVPNKGVMYTFVCKCWFAKDRGDGLTARVFNMLDATAITIGHKILYEVTVVTGDVPNGGTDANIYITCFGASGSSEEMQLPKGDDRFERGQRDTFVMEIADIAPLKKMRLRIDGKGSRPEWYFEQISLKNLTTEEETVFMYGDWMSKTKGERKSLSCEMAATVDGEEMVETTTYIIQVKTSDVSGAGTDANVFLIIFGENGDTGTLALKQSNNSNKFERNQSDVFRFPDMLSLGDLCKVRVWHDNSGIGPGWHLEFIDIKDETMSKSFRFQCDRWLAKNADDHQIIRELACANNDILDLKEKTSYEIVTVTSDRDDAETKDNVWIVLEGKKGRSKEFLMENTSKKRRFARDATDTFKFSSKNLGDIAAICVGHCPKDGRKFSPKPDLYWHVKEIIVTETELGNKYVFNCNAKVPLRSKRDDYRVFECAKAIESFASRARSLVPVKYEVIVVTGFEKGAGTDANVTLVMYGSNGDSGKHALKQKMRNLFERGKTNRFYIETLDLGEMKKVRVEHDNSGLSPGWLLERVEVTNSATGVTTIFPCGKWLDKKRGDGETWRELYPKY

Target ORF information:

RefSeq Version	XM_018090855.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis lipoxygenase homology domains 1 (loxhd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018090855.1

ORF Insert Sequence:

ATGAAGAAAG AAGACGAAGG GCAGGACCAG TCTCAAGCCA CACTGGGTTT GGAACAGAAA 
GCCAAATCGA CAACTTACAC AGTGACAATA AAGACCGGAG ACAAGAAGAA TTCTGGGACA 
GATGCCAATG TATTCTTAAT CTTGTATGGA GAGAAAGATG ATACAGGGTT CATGAACCTC 
AAGGCTTCCA AGACCAACAA GAATAAGTTT GAGCGAGGCC AAACTGATGT GTTTACCGTG 
GAGGCCGTGG ATATCGGGAC TCTCGCCAAA CTAAAAATTG GGCACGATAA CAAAGGCACC 
TCGGCTGGTT GGTTCCTGGA CTGGGTAGAG ATTGATGCAC CATCACTTGG TCAGCGGGTT 
AAGTTCCCCT GTGGTCGCTG GTTGGATAAA AGTGAAGATG ATGGGACCAT CGAAAGATAT 
CTGTTCCCAT CTGAACTGCA AACTGAGCAG TATGTTCCAT TTGTTCCGTA CGAGATCACA 
GTCTACACCA GCGATATATT CGCGGCCGGC ACGGACGCCG ACGTCTTTAT CGTTCTGTAT 
GGCCGAGACG GAGTCTGTAC CCAGCAGAAA TCCCTGTGTG TGAACAAGAG AGAGCGGAGA 
TTGTACTTTG AGCGAAACTC GGTGGATCAG TTCATTGTAG AGCTAGAAGA CGTTGGAGAT 
ATCATTGAGA AGATTCGCAT TGGGCATACT GACAAAGGCA TGAGCGCAGG GTGGCATTTA 
GACAGAGTGG AAATTCGTAG ACTGCTGCCA AATGGAAAGG GCTCCCAGAC CGTAACCTTT 
CCGTGTGAGC GGTGGCTTGC CAAATCCGAG GATGACGGAG AGACAGTGAG AGAACTAGTG 
CCATCCGATA TCTTTACTGA GAAACTCATG AAAGATGGGA CCTTGAAACA GATTGAGATG 
GAAGTAGAAG ACCCTCTGGA AACCCACACG TACAGCATAT CTGTGTTCAC CGGCGACATC 
TATGGTTCGG GTACCGACGC CAACGTCTTC ATCACCATTT ACGGAGATTT GGGAGACACG 
GGAGAGAGAA AACTCAGCAA GTCCGAGACC AACTCCAACA AGTTTGAGAG GGGACAGGTG 
GATAAATTCA ATATAGAAGC GGTGGATCTC GGCACCATCT ATAAGATTAG GATCCGGCAC 
GATAACTCTA TGTTGAACCC TGACTGGTAC CTGGAGAGGG TAGAGATCCT TAATGAAGTC 
ACGGAGGAGA CGTATCTGTT CATCTGTGAG CGATGGCTCT CCACCAAGAA GGAAGACAAG 
AAGATTGAAC GAATATTGTA TGAGAAGGAG TATGACGGGG AGCGACTCAG CTTGGGCAGC 
TCATCCAAAA GTTTCTGGGG ATCATCCCTC AGCCTGAGAA GTCAGGATAA CAATGCCAAT 
GTAAAGAGCA GGAACAGCCT AGATGGCTCC CAGCAAGATG GAACATTAAT CCCGTATCAC 
ATCACTATCA CCACCGGCCT TGACCGAGTT GGTGGGACAG AAAGTCGTGC TTATGTCATT 
ATAATGGGAC CTCAGAAGAT AAGGACAGAG CGACTTTGGC TGGACCTGCC CAAAGATAAG 
AAAGGCTTTG CGCCAGGGTC CGTGGAGAAG TTCTCTGTGG TGGGACTGGA TGTCGGAGAG 
ATTAAAAAGA TGGAGTTGGG CCATGATGGG GTTACCCCAG AAAGCTGCTG GCTAGTGGAT 
GAGATCCAAC TAACAGTGCC TAACAAAGGA GTCATGTATA CCTTTGTGTG TAAATGCTGG 
TTTGCCAAAG ATAGAGGAGA CGGTCTAACT GCCCGGGTCT TCAATATGCT GGATGCAACT 
GCCATCACTA TTGGCCATAA GATTCTCTAT GAGGTTACAG TTGTCACAGG AGATGTACCA 
AATGGGGGTA CTGATGCCAA TATTTATATT ACCTGCTTTG GAGCAAGTGG GAGCTCAGAG 
GAAATGCAGC TGCCAAAAGG GGATGACAGG TTTGAGAGAG GCCAGCGTGA CACTTTTGTC 
ATGGAGATCG CTGACATTGC CCCACTGAAG AAGATGAGAT TGCGTATCGA TGGGAAGGGC 
AGCCGCCCTG AGTGGTATTT CGAACAGATC TCCTTGAAGA ACTTGACCAC CGAAGAGGAG 
ACAGTGTTCA TGTATGGGGA CTGGATGTCC AAGACCAAGG GGGAGAGGAA GTCGCTCTCC 
TGTGAAATGG CGGCCACAGT AGACGGCGAA GAAATGGTGG AAACTACAAC GTACATTATA 
CAAGTCAAAA CCAGCGACGT GTCAGGAGCC GGTACCGACG CCAACGTGTT CCTGATCATC 
TTTGGAGAGA ACGGAGACAC CGGCACTCTG GCGCTGAAGC AGTCCAACAA TTCCAACAAG 
TTCGAAAGGA ATCAGAGTGA TGTCTTCAGA TTCCCTGACA TGTTGAGCCT CGGAGACCTG 
TGCAAAGTCC GGGTCTGGCA CGATAACTCA GGTATTGGCC CCGGCTGGCA CCTGGAATTT 
ATCGACATAA AGGACGAGAC CATGAGCAAG AGTTTCCGCT TCCAGTGCGA CCGATGGCTG 
GCCAAGAACG CCGATGACCA TCAGATCATC AGGGAACTCG CCTGTGCCAA CAACGATATC 
CTGGATCTGA AAGAGAAAAC CTCTTATGAG ATTGTGACGG TGACAAGCGA CAGGGATGAT 
GCAGAGACCA AGGACAACGT CTGGATCGTC TTGGAGGGCA AAAAAGGGAG GTCAAAAGAA 
TTTCTAATGG AGAACACTTC CAAAAAGCGG AGGTTTGCTC GGGATGCTAC AGATACATTC 
AAGTTTTCCT CCAAGAACCT TGGTGACATC GCTGCCATCT GCGTTGGCCA TTGCCCTAAG 
GACGGAAGGA AGTTTTCTCC GAAGCCGGAT TTATACTGGC ACGTCAAGGA GATCATCGTG 
ACCGAGACCG AACTCGGAAA CAAGTATGTG TTTAACTGCA ACGCCAAGGT GCCGCTGCGC 
AGCAAGCGAG ACGACTACAG AGTGTTCGAG TGTGCCAAGG CCATCGAGAG CTTCGCCAGC 
CGCGCCCGCA GTCTGGTGCC CGTCAAATAC GAAGTCATCG TGGTCACCGG GTTCGAAAAG 
GGGGCGGGGA CGGACGCCAA CGTGACCCTC GTCATGTACG GCTCCAACGG GGACTCCGGG 
AAGCACGCGC TGAAGCAGAA GATGCGCAAC CTCTTCGAGC GCGGGAAAAC CAACCGCTTC 
TACATAGAGA CCCTCGACCT GGGGGAGATG AAGAAAGTGC GGGTGGAGCA CGATAACAGC 
GGCCTGAGCC CCGGCTGGCT ACTCGAGCGG GTGGAGGTGA CCAACAGCGC CACCGGCGTC 
ACCACCATTT TCCCCTGCGG CAAGTGGCTG GACAAGAAAC GGGGCGACGG CGAGACCTGG 
AGGGAACTCT ACCCCAAATA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

loxhd1 ( XM_018090855.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017946344.1 Xenopus tropicalis lipoxygenase homology domains 1 (loxhd1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa49272
Clone ID Related Accession (Same CDS sequence)	XM_018090855.2
Accession Version	XM_018090855.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	lipoxygenase homology domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018090855.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAG AAGACGAAGG GCAGGACCAG TCTCAAGCCA CACTGGGTTT GGAACAGAAA 
GCCAAATCGA CAACTTACAC AGTGACAATA AAGACCGGAG ACAAGAAGAA TTCTGGGACA 
GATGCCAATG TATTCTTAAT CTTGTATGGA GAGAAAGATG ATACAGGGTT CATGAACCTC 
AAGGCTTCCA AGACCAACAA GAATAAGTTT GAGCGAGGCC AAACTGATGT GTTTACCGTG 
GAGGCCGTGG ATATCGGGAC TCTCGCCAAA CTAAAAATTG GGCACGATAA CAAAGGCACC 
TCGGCTGGTT GGTTCCTGGA CTGGGTAGAG ATTGATGCAC CATCACTTGG TCAGCGGGTT 
AAGTTCCCCT GTGGTCGCTG GTTGGATAAA AGTGAAGATG ATGGGACCAT CGAAAGATAT 
CTGTTCCCAT CTGAACTGCA AACTGAGCAG TATGTTCCAT TTGTTCCGTA CGAGATCACA 
GTCTACACCA GCGATATATT CGCGGCCGGC ACGGACGCCG ACGTCTTTAT CGTTCTGTAT 
GGCCGAGACG GAGTCTGTAC CCAGCAGAAA TCCCTGTGTG TGAACAAGAG AGAGCGGAGA 
TTGTACTTTG AGCGAAACTC GGTGGATCAG TTCATTGTAG AGCTAGAAGA CGTTGGAGAT 
ATCATTGAGA AGATTCGCAT TGGGCATACT GACAAAGGCA TGAGCGCAGG GTGGCATTTA 
GACAGAGTGG AAATTCGTAG ACTGCTGCCA AATGGAAAGG GCTCCCAGAC CGTAACCTTT 
CCGTGTGAGC GGTGGCTTGC CAAATCCGAG GATGACGGAG AGACAGTGAG AGAACTAGTG 
CCATCCGATA TCTTTACTGA GAAACTCATG AAAGATGGGA CCTTGAAACA GATTGAGATG 
GAAGTAGAAG ACCCTCTGGA AACCCACACG TACAGCATAT CTGTGTTCAC CGGCGACATC 
TATGGTTCGG GTACCGACGC CAACGTCTTC ATCACCATTT ACGGAGATTT GGGAGACACG 
GGAGAGAGAA AACTCAGCAA GTCCGAGACC AACTCCAACA AGTTTGAGAG GGGACAGGTG 
GATAAATTCA ATATAGAAGC GGTGGATCTC GGCACCATCT ATAAGATTAG GATCCGGCAC 
GATAACTCTA TGTTGAACCC TGACTGGTAC CTGGAGAGGG TAGAGATCCT TAATGAAGTC 
ACGGAGGAGA CGTATCTGTT CATCTGTGAG CGATGGCTCT CCACCAAGAA GGAAGACAAG 
AAGATTGAAC GAATATTGTA TGAGAAGGAG TATGACGGGG AGCGACTCAG CTTGGGCAGC 
TCATCCAAAA GTTTCTGGGG ATCATCCCTC AGCCTGAGAA GTCAGGATAA CAATGCCAAT 
GTAAAGAGCA GGAACAGCCT AGATGGCTCC CAGCAAGATG GAACATTAAT CCCGTATCAC 
ATCACTATCA CCACCGGCCT TGACCGAGTT GGTGGGACAG AAAGTCGCGC TTATGTCATC 
ATAATGGGAC CTCAGAAGAT AAGGACAGAG CGACTTTGGC TGGACCTGCC CAAAGATAAG 
AAGGGCTTTG CGCCAGGGTC TGTGGAGAAG TTCTCTGTGG TGGGATTGGA TGTCGGAGAG 
ATTAAAAAGA TGGAGTTGGG CCATGATGGG GTTACCCCAG AAAGCTGCTG GCTGGTGGAT 
GAGATCCAAC TAACAGTGCC TAACAAAGGA GTCATGTATA CCTTTGTCTG TAAATGCTGG 
TTTGCCAAAG ATAGAGGAGA CGGTCTAACT GCCCGGGTCT TCAATATGCT GGATGCAACT 
GCCATCACTA TTGGCCATAA GATTCTCTAT GAGGTTACAG TTGTCACAGG AGATGTACCA 
AATGGGGGTA CTGATGCCAA TATTTATATT ACCTGCTTTG GAGCAAGTGG GAGCTCAGAG 
GAAATGCAGC TGCCAAAAGG GGATGACAGG TTTGAGAGAG GCCAGCGTGA CACTTTTGTC 
ATGGAGATCG CTGACATTGC CCCACTGAAG AAGATGAGAT TGCGTATCGA TGGGAAGGGC 
AGCCGCCCTG AGTGGTATTT CGAACAGATC TCCTTGAAGA ACTTGACCAC CGAAGAGGAG 
ACAGTGTTCA TGTATGGGGA CTGGATGTCC AAGACCAAGG GGGAGAGGAA GTCGCTCTCC 
TGTGAAATGG CGGCCACAGT AGACGGCGAA GAAATGGTGG AAACTACAAC ATACATTATA 
CAAGTCAAAA CCAGCGACGT GTCAGGAGCC GGTACCGACG CCAACGTGTT CCTGATCATC 
TTTGGAGAGA ACGGAGACAC CGGCACTCTG GCGCTGAAGC AGTCCAACAA TTCCAACAAG 
TTCGAAAGGA ATCAGAGTGA TGTCTTCAGA TTCCCTGACA TGTTGAGCCT CGGAGACCTG 
TGCAAAGTCC GGGTCTGGCA CGATAACTCA GGTATTGGCC CCGGCTGGCA CCTGGAATTT 
ATCGACATAA AGGACGAGAC CATGAGCAAG AGTTTCCGCT TCCAGTGCGA CCGATGGCTG 
GCCAAGAACG CCGATGACCA TCAGATCATC AGGGAACTCG CCTGTGCCAA CAACGATATC 
CTGGATCTGA AAGAGAAAAC CTCTTATGAG ATTGTGACGG TGACAAGCGA CAGGGATGAT 
GCAGAGACCA AGGACAACGT CTGGATCGTC TTGGAGGGCA AAAAAGGGAG GTCAAAAGAA 
TTTCTAATGG AGAACACTTC CAAAAAGCGG AGGTTTGCTC GGGATGCAAC CGATACATTC 
AAGTTTTCCT CCAAGAACCT TGGTGACATC GCTGCCATCT GCGTTGGCCA TTGCCCTAAG 
GACGGAAGGA AGTTTTCTCC GAAGCCGGAT TTATACTGGC ACGTCAAGGA GATCATCGTG 
ACCGAGACCG AACTCGGAAA CAAGTATGTG TTTAACTGCA ACGCCAAGGT GCCGCTGCGC 
AGCAAGCGAG ACGACTACAG AGTGTTCGAG TGTGCCAAGG CCATCGAGAG CTTCGCCAGC 
CGCGCCCGCA GTCTGGTGCC CGTCAAATAC GAAGTCATCG TGGTCACCGG GTTCGAAAAG 
GGGGCGGGGA CGGACGCCAA CGTGACCCTC GTCATGTACG GCTCCAACGG GGACTCCGGG 
AAGCACGCGC TGAAGCAGAA GATGCGCAAC CTCTTCGAGC GCGGGAAAAC CAACCGCTTC 
TACATAGAGA CCCTCGACCT GGGGGAGATG AAGAAAGTGC GGGTGGAGCA CGATAACAGC 
GGCCTGAGCC CCGGCTGGCT ACTCGAGCGG GTGGAGGTGA CCAACAGCGC CACCGGCGTC 
ACCACCATAT TCCCCTGCGG CAAGTGGCTG GACAAGAAAC GGGGCGACGG CGAGACCTGG 
AGGGAACTCT ACCCCAAATA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017946344.1
CDS	160..3543
Translation	MKKEDEGQDQSQATLGLEQKAKSTTYTVTIKTGDKKNSGTDANVFLILYGEKDDTGFMNLKASKTNKNKFERGQTDVFTVEAVDIGTLAKLKIGHDNKGTSAGWFLDWVEIDAPSLGQRVKFPCGRWLDKSEDDGTIERYLFPSELQTEQYVPFVPYEITVYTSDIFAAGTDADVFIVLYGRDGVCTQQKSLCVNKRERRLYFERNSVDQFIVELEDVGDIIEKIRIGHTDKGMSAGWHLDRVEIRRLLPNGKGSQTVTFPCERWLAKSEDDGETVRELVPSDIFTEKLMKDGTLKQIEMEVEDPLETHTYSISVFTGDIYGSGTDANVFITIYGDLGDTGERKLSKSETNSNKFERGQVDKFNIEAVDLGTIYKIRIRHDNSMLNPDWYLERVEILNEVTEETYLFICERWLSTKKEDKKIERILYEKEYDGERLSLGSSSKSFWGSSLSLRSQDNNANVKSRNSLDGSQQDGTLIPYHITITTGLDRVGGTESRAYVIIMGPQKIRTERLWLDLPKDKKGFAPGSVEKFSVVGLDVGEIKKMELGHDGVTPESCWLVDEIQLTVPNKGVMYTFVCKCWFAKDRGDGLTARVFNMLDATAITIGHKILYEVTVVTGDVPNGGTDANIYITCFGASGSSEEMQLPKGDDRFERGQRDTFVMEIADIAPLKKMRLRIDGKGSRPEWYFEQISLKNLTTEEETVFMYGDWMSKTKGERKSLSCEMAATVDGEEMVETTTYIIQVKTSDVSGAGTDANVFLIIFGENGDTGTLALKQSNNSNKFERNQSDVFRFPDMLSLGDLCKVRVWHDNSGIGPGWHLEFIDIKDETMSKSFRFQCDRWLAKNADDHQIIRELACANNDILDLKEKTSYEIVTVTSDRDDAETKDNVWIVLEGKKGRSKEFLMENTSKKRRFARDATDTFKFSSKNLGDIAAICVGHCPKDGRKFSPKPDLYWHVKEIIVTETELGNKYVFNCNAKVPLRSKRDDYRVFECAKAIESFASRARSLVPVKYEVIVVTGFEKGAGTDANVTLVMYGSNGDSGKHALKQKMRNLFERGKTNRFYIETLDLGEMKKVRVEHDNSGLSPGWLLERVEVTNSATGVTTIFPCGKWLDKKRGDGETWRELYPKY

Target ORF information:

RefSeq Version	XM_018090855.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis lipoxygenase homology domains 1 (loxhd1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018090855.2

ORF Insert Sequence:

ATGAAGAAAG AAGACGAAGG GCAGGACCAG TCTCAAGCCA CACTGGGTTT GGAACAGAAA 
GCCAAATCGA CAACTTACAC AGTGACAATA AAGACCGGAG ACAAGAAGAA TTCTGGGACA 
GATGCCAATG TATTCTTAAT CTTGTATGGA GAGAAAGATG ATACAGGGTT CATGAACCTC 
AAGGCTTCCA AGACCAACAA GAATAAGTTT GAGCGAGGCC AAACTGATGT GTTTACCGTG 
GAGGCCGTGG ATATCGGGAC TCTCGCCAAA CTAAAAATTG GGCACGATAA CAAAGGCACC 
TCGGCTGGTT GGTTCCTGGA CTGGGTAGAG ATTGATGCAC CATCACTTGG TCAGCGGGTT 
AAGTTCCCCT GTGGTCGCTG GTTGGATAAA AGTGAAGATG ATGGGACCAT CGAAAGATAT 
CTGTTCCCAT CTGAACTGCA AACTGAGCAG TATGTTCCAT TTGTTCCGTA CGAGATCACA 
GTCTACACCA GCGATATATT CGCGGCCGGC ACGGACGCCG ACGTCTTTAT CGTTCTGTAT 
GGCCGAGACG GAGTCTGTAC CCAGCAGAAA TCCCTGTGTG TGAACAAGAG AGAGCGGAGA 
TTGTACTTTG AGCGAAACTC GGTGGATCAG TTCATTGTAG AGCTAGAAGA CGTTGGAGAT 
ATCATTGAGA AGATTCGCAT TGGGCATACT GACAAAGGCA TGAGCGCAGG GTGGCATTTA 
GACAGAGTGG AAATTCGTAG ACTGCTGCCA AATGGAAAGG GCTCCCAGAC CGTAACCTTT 
CCGTGTGAGC GGTGGCTTGC CAAATCCGAG GATGACGGAG AGACAGTGAG AGAACTAGTG 
CCATCCGATA TCTTTACTGA GAAACTCATG AAAGATGGGA CCTTGAAACA GATTGAGATG 
GAAGTAGAAG ACCCTCTGGA AACCCACACG TACAGCATAT CTGTGTTCAC CGGCGACATC 
TATGGTTCGG GTACCGACGC CAACGTCTTC ATCACCATTT ACGGAGATTT GGGAGACACG 
GGAGAGAGAA AACTCAGCAA GTCCGAGACC AACTCCAACA AGTTTGAGAG GGGACAGGTG 
GATAAATTCA ATATAGAAGC GGTGGATCTC GGCACCATCT ATAAGATTAG GATCCGGCAC 
GATAACTCTA TGTTGAACCC TGACTGGTAC CTGGAGAGGG TAGAGATCCT TAATGAAGTC 
ACGGAGGAGA CGTATCTGTT CATCTGTGAG CGATGGCTCT CCACCAAGAA GGAAGACAAG 
AAGATTGAAC GAATATTGTA TGAGAAGGAG TATGACGGGG AGCGACTCAG CTTGGGCAGC 
TCATCCAAAA GTTTCTGGGG ATCATCCCTC AGCCTGAGAA GTCAGGATAA CAATGCCAAT 
GTAAAGAGCA GGAACAGCCT AGATGGCTCC CAGCAAGATG GAACATTAAT CCCGTATCAC 
ATCACTATCA CCACCGGCCT TGACCGAGTT GGTGGGACAG AAAGTCGCGC TTATGTCATC 
ATAATGGGAC CTCAGAAGAT AAGGACAGAG CGACTTTGGC TGGACCTGCC CAAAGATAAG 
AAGGGCTTTG CGCCAGGGTC TGTGGAGAAG TTCTCTGTGG TGGGATTGGA TGTCGGAGAG 
ATTAAAAAGA TGGAGTTGGG CCATGATGGG GTTACCCCAG AAAGCTGCTG GCTGGTGGAT 
GAGATCCAAC TAACAGTGCC TAACAAAGGA GTCATGTATA CCTTTGTCTG TAAATGCTGG 
TTTGCCAAAG ATAGAGGAGA CGGTCTAACT GCCCGGGTCT TCAATATGCT GGATGCAACT 
GCCATCACTA TTGGCCATAA GATTCTCTAT GAGGTTACAG TTGTCACAGG AGATGTACCA 
AATGGGGGTA CTGATGCCAA TATTTATATT ACCTGCTTTG GAGCAAGTGG GAGCTCAGAG 
GAAATGCAGC TGCCAAAAGG GGATGACAGG TTTGAGAGAG GCCAGCGTGA CACTTTTGTC 
ATGGAGATCG CTGACATTGC CCCACTGAAG AAGATGAGAT TGCGTATCGA TGGGAAGGGC 
AGCCGCCCTG AGTGGTATTT CGAACAGATC TCCTTGAAGA ACTTGACCAC CGAAGAGGAG 
ACAGTGTTCA TGTATGGGGA CTGGATGTCC AAGACCAAGG GGGAGAGGAA GTCGCTCTCC 
TGTGAAATGG CGGCCACAGT AGACGGCGAA GAAATGGTGG AAACTACAAC ATACATTATA 
CAAGTCAAAA CCAGCGACGT GTCAGGAGCC GGTACCGACG CCAACGTGTT CCTGATCATC 
TTTGGAGAGA ACGGAGACAC CGGCACTCTG GCGCTGAAGC AGTCCAACAA TTCCAACAAG 
TTCGAAAGGA ATCAGAGTGA TGTCTTCAGA TTCCCTGACA TGTTGAGCCT CGGAGACCTG 
TGCAAAGTCC GGGTCTGGCA CGATAACTCA GGTATTGGCC CCGGCTGGCA CCTGGAATTT 
ATCGACATAA AGGACGAGAC CATGAGCAAG AGTTTCCGCT TCCAGTGCGA CCGATGGCTG 
GCCAAGAACG CCGATGACCA TCAGATCATC AGGGAACTCG CCTGTGCCAA CAACGATATC 
CTGGATCTGA AAGAGAAAAC CTCTTATGAG ATTGTGACGG TGACAAGCGA CAGGGATGAT 
GCAGAGACCA AGGACAACGT CTGGATCGTC TTGGAGGGCA AAAAAGGGAG GTCAAAAGAA 
TTTCTAATGG AGAACACTTC CAAAAAGCGG AGGTTTGCTC GGGATGCAAC CGATACATTC 
AAGTTTTCCT CCAAGAACCT TGGTGACATC GCTGCCATCT GCGTTGGCCA TTGCCCTAAG 
GACGGAAGGA AGTTTTCTCC GAAGCCGGAT TTATACTGGC ACGTCAAGGA GATCATCGTG 
ACCGAGACCG AACTCGGAAA CAAGTATGTG TTTAACTGCA ACGCCAAGGT GCCGCTGCGC 
AGCAAGCGAG ACGACTACAG AGTGTTCGAG TGTGCCAAGG CCATCGAGAG CTTCGCCAGC 
CGCGCCCGCA GTCTGGTGCC CGTCAAATAC GAAGTCATCG TGGTCACCGG GTTCGAAAAG 
GGGGCGGGGA CGGACGCCAA CGTGACCCTC GTCATGTACG GCTCCAACGG GGACTCCGGG 
AAGCACGCGC TGAAGCAGAA GATGCGCAAC CTCTTCGAGC GCGGGAAAAC CAACCGCTTC 
TACATAGAGA CCCTCGACCT GGGGGAGATG AAGAAAGTGC GGGTGGAGCA CGATAACAGC 
GGCCTGAGCC CCGGCTGGCT ACTCGAGCGG GTGGAGGTGA CCAACAGCGC CACCGGCGTC 
ACCACCATAT TCCCCTGCGG CAAGTGGCTG GACAAGAAAC GGGGCGACGG CGAGACCTGG 
AGGGAACTCT ACCCCAAATA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

loxhd1 ( XM_031894937.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031750797.1 Xenopus tropicalis lipoxygenase homology domains 1 (loxhd1), transcript variant X1, mRNA.

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CloneID	OXa49271
Clone ID Related Accession (Same CDS sequence)	XM_031894937.1
Accession Version	XM_031894937.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	lipoxygenase homology domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGACCC AGATCCAGTA TTACAACTCG TCCCCGTCAG TCACGGGTGT GCTCCAGCCT 
TGCACTGCGC CCGCTTCTTT TGGGCAGATG AGACCAAGGG ATGCCTATCC CAGTACCACG 
GCACCTCGGA TCCGACCAGC CACTGGGTTC CAGTTCCGAG ATGTCTCCCC TCCCCGGGGG 
AAGCCTGTGT GGAACGACGG ATTCAAGTCA GAGGGCTGGA GAAACTCCCT GCGCAGATAT 
AAAGTCAGTC TGAACCACAG GCCCGTGGAG TCCATGCTAA GCAAACGAGC TCCATATGCA 
GGAAGAACTC TAAGTGCCAG CTCCGGCGGG TATCGGTACA GAAACGGGCA CACAGACACT 
CTGCCGGCGT ATAGCGCTAT GAACGACCCT CATTTATATG ATTTCTATGC CAAGAAGTTT 
GGATTGCAGG TGAACCCCCA GCCGCCCTCA CTGAAACGGG CCAAGAGCGC CCCCTCCAGA 
AGATCTGTGC TCCGCTCCGG TTCTCTCTCT GGGAAGAAGG TTCTGTACAA GATTGTGGTG 
AAGACTGGAG ACAAGAAGCA GGCCGGCACT GACGCAAGGG TATTTCTCCA GATAAAGGGA 
TCCAAAGGAA AGCTCAGTAA GACCCAGCTG TATAAAGCAT CTGGAACAGA CTCACCCGAC 
AAGGCAGCAA CTTTTGCATT CAAAAAGGGC TCCACCAACA CATTCAAAGT GTACGGCCCC 
GAAATCGGAG ACATCAGGAC GATCACAATT GAGCACGATG GCCTGGAGCA GCGGCACGCT 
TGGTTCCTGG AGGAAGTTTT TGTAACCAAT ACTGATCGGG ACAAATCCTG GTACTTCCCA 
TGCCGGAGAT GGTTCTCCCT GTACCACACA GACTGCCAGC TGAGCCGCAC TTTATCCCCG 
GAGATTAAAC CCGTATCTCA TGAACTTGAT GGCAAAAAAG GGGACCACAC TGTATACGAG 
GTGGTCACTG TTACAGGAGA CGTCCGGGGG GCCGGCACCG ACGCCAATGT GTTTGTCACC 
CTTTTCGGAG AATTCGGAAT CACTCCCAAG ATTCACCTCA CCAGCAAGAG TAGGACTGCC 
TTCGAACGGT CAAAGACTGA TGTGTTTAGA GTCAAAACCA ATAACGTCGG ACCACTCAAG 
AAGATCAGAA TTGAGCATGA TAACACAGGA ATGAATGCAG GATGGTATCT GGATCGGGCC 
ATCATAACGG ATATGAACAG GCCCCATCTC CGGTTCTACT TCCCCTGCAA CAACTGGCTG 
AGCAAGGAAG ATGGCGATGG GCTTTACGTC AGGGATCTGC TGGGCAGTCT CAATCCCATG 
GATGTGCCCA AAATGAACAA ATATGTTGCA AGAGTCTTTA CTGGTGCAGC CAATGGCAGT 
GGGACTGATG CAGATGTTTT CCTCAATATT TTTGGGGAGA ATGGAGACAC AGGGGAGCGG 
AAACTTGACA ATGATAAAAA CAACTTTGAG AAGGGGTCAG AAGACAAATT TACTATTGAT 
GCGCCAAATC TGGGGAAAAT AAGGAAAATC ACTATAGGTC ACAACAACAA AGGAGGCTCG 
GCAGGCTGGT TCCTGGAGAA GGTTATCATT GAAGATATTG GAAACAAAAC CGTTTATGAG 
TTCCCCTGCT CTCGCTGGCT CGCTATCGAT GAAGATGATG GAAAGATTCA GCGAGATCTG 
CTTGTCGGAG GGAGTGAAGC AACAGGCATT GTCTACAACG TTGCAATTAT GACTGGAGAT 
ATCCGAGGTG CCGGAACAGA TTCCAAGATC CACGTTATCC TCCATGGAGA GAAAGGTGTG 
AAGAACAGCG GCAAACTCTT CTTGGAAGGA GGGGAATTCG AGAGAGGGAG GATAGACATT 
TTCAACATTG AAATCGCAGC GCTACTGAGC CCCCTCAGCA GAGTGACAAT TGGACATGAT 
GGAAACGGTG TAGGGGCTGG ATGGTACTGT GAGAAGGTTA TAGTTTACTG CCCATTCACC 
GGCATTGAAC AAACATTTCC ATGTGGCCAT TGGTTGGATG TAGACGAAGG AGATGGCTTA 
ATAGAGAGAG AACTCTATGA AATGTTGTCC TTACGCCAGA AGAGGCAGAA GAAACATCCA 
TGGTCCCTTT GGATATGGAC GAGCGATATG AAAAGTGCCG GAACCGATGC CAACATCCTT 
CTTCAGGTCT ACGGAGAGAA AGGAAAGTCT GATGAAATAA AACTGGACAA TAACTCCGAC 
AACTTTGAAG CTGGACAGGT CGACAGGTTC ATGATTGAAC TTCCTGATCT GGGAACTCTG 
TACAAAATCC GAGTTTGGCA CGAGAAGAGA GCGGCCTTTT CCGGTTGGCA TTTAAACAAG 
GTAACCCTGC TGAAGACACT TACCAAAGAG AAATATACCT TTAAATGCGG TCGCTGGTTG 
GACCTGAATG AAGACGACCA TGAAATTGTA AGAGAGCTTC CAGGAGAGGG GCCACTTATA 
GACTCCGTCC TGCCTGTGGT AAAGTATCGC GTGACTGTGT ACACTGGTAC CGTTAGCGGG 
AGCGGCACCG ATGCCAATGT GTTTATCTGT CTCTTTGGTG AACTCGGCGA CACAGGAGAG 
CGTTTTCTCA TCGAATGCAA AAACAATATG AATAAGTTTG AGAAAGGCAA TGCTGATGAC 
TTTATTATTG AAGCGGTGAC CCTGAAGCAG GTGAAAAGAG TGCGGATCGG ACACGATGGG 
AAAGGAGGAG GCTGTGGTTG GTATATGGAC AAAGTTGTAG TGAGAGAAGA TGGGAAACCG 
GAGACAGAGG CTATTGAATT TCCTTGCAAC AGGTGGTTTG ACAGAAATGA GGATGACGGT 
CACATCATTA GGGAGCTGGT TCCCGCCGGA GACTCTCAGA ATCTGAGGAG TATCAGCTAT 
CACTTTCAGA TCAAAACGGG GGACGTCAGC GGAGCCAGCT CAGATTCCAG GGTCTTCGTC 
AAGCTCTACG GAGAGAAGGG GGACACCAAC AAGCAGTTCC TGCTGGTGTC CGATAACGAC 
CTGGCGGATA ACTTTGAGAG GGGCCGAGTG GATAATTTCA CCATAAATAC CATCGATATA 
GGGAAGATCA ATAAGCTGTT GATTGGCCAT GACAATGTAG GGATTCGAGC GGGTTGGTTC 
CTGGCCAACG TTGTGGTTCA GGTCCCCGCC CATGGCAAGG AGTACATGTT TCCCGTTAAC 
CGCTGGCTGT GCAAAGACGA GTGCGACGGC AAGGTGGAGG TGGAAGTGTA CGCTAGCGAG 
GTGAAAAGCA TTGAGAAAAT GGTAAATTAT GAAGTGGTAG TAACCACCGG CAACGTACGT 
GGATCTGGAA CCAACGCCAA CGTCTTCATG CAACTCTACG GGGAACAGGG GAAGACGGAG 
GAAACGTTTT TAAGAAGCCG TTCTAATGAC TTTGAAAGAG GAGCTGTAGA TATATTTAAG 
ATCGAGGCCG CAGAAGTGGG TAAGATCCGC AAGCTCAGGA TTGGCCACGA TGGCAGCGGC 
TTTGGAGACG GCTGGTATTT GGAGAGCGTT GAGATCAAGC GAGTCGGAAT AAAGATGATG 
CCTGTGGAGA AGCCGGAGAA GAAGAAAAAA CCCAACAAGA AGAAGAAATC CAGTAAGGAG 
GAAGAAGAGG AGCTTCAGCC TCGAGAAGTG GTGGAGTCCT ACAAGTTTGA GTGCAAGCGC 
TGGTTGGCCA GGGGGGAAGA AGATGGAGAG CTAGTGGTTG AACTTCTGCC TCTGGAGACT 
TCAGAAATAG AAGAGAACAG CTACGAGGTC CATGTATTTA CCGGTACCGT TTGGGGAGCT 
GGAACAGACG CCAATGTTTA TGCCGCCATA TATGGAGAAT TAGGAGACAC GGGAGAACGT 
CAGCTAAAGA AAGCCAACCA TCTGAACAAA TTTGAGAGGG GCCAGGAGGA TATATTCACT 
ATTAAAGCTG TAGATTTAGG AGAGCTTAAG AAGCTGAAAA TCCGCCACGA CAACAAGGGT 
GCCAACGCTG GGTGGTTCCT CGACAGAGTA GAGATTGTGG ATGAGAAGGA CGACACAAAG 
TATTTCTTCC CATGCCAGAG ATGGCTTGCT GTCGATGAAG ACGACGGACA GATCGCCAGG 
CAGCTGGTCC CTGTGGACGA GGCCTTTATG AAGAAAGAAG ACGAAGGGCA GGACCAGTCT 
CAAGCCACAC TGGGTTTGGA ACAGAAAGCC AAATCGACAA CTTACACAGT GACAATAAAG 
ACCGGAGACA AGAAGAATTC TGGGACAGAT GCCAATGTAT TCTTAATCTT GTATGGAGAG 
AAAGATGATA CAGGGTTCAT GAACCTCAAG GCTTCCAAGA CCAACAAGAA TAAGTTTGAG 
CGAGGCCAAA CTGATGTGTT TACCGTGGAG GCCGTGGATA TCGGGACTCT CGCCAAACTA 
AAAATTGGGC ACGATAACAA AGGCACCTCG GCTGGTTGGT TCCTGGACTG GGTAGAGATT 
GATGCACCAT CACTTGGTCA GCGGGTTAAG TTCCCCTGTG GTCGCTGGTT GGATAAAAGT 
GAAGATGATG GGACCATCGA AAGATATCTG TTCCCATCTG AACTGCAAAC TGAGCAGTAT 
GTTCCATTTG TTCCGTACGA GATCACAGTC TACACCAGCG ATATATTCGC GGCCGGCACG 
GACGCCGACG TCTTTATCGT TCTGTATGGC CGAGACGGAG TCTGTACCCA GCAGAAATCC 
CTGTGTGTGA ACAAGAGAGA GCGGAGATTG TACTTTGAGC GAAACTCGGT GGATCAGTTC 
ATTGTAGAGC TAGAAGACGT TGGAGATATC ATTGAGAAGA TTCGCATTGG GCATACTGAC 
AAAGGCATGA GCGCAGGGTG GCATTTAGAC AGAGTGGAAA TTCGTAGACT GCTGCCAAAT 
GGAAAGGGCT CCCAGACCGT AACCTTTCCG TGTGAGCGGT GGCTTGCCAA ATCCGAGGAT 
GACGGAGAGA CAGTGAGAGA ACTAGTGCCA TCCGATATCT TTACTGAGAA ACTCATGAAA 
GATGGGACCT TGAAACAGAT TGAGATGGAA GTAGAAGACC CTCTGGAAAC CCACACGTAC 
AGCATATCTG TGTTCACCGG CGACATCTAT GGTTCGGGTA CCGACGCCAA CGTCTTCATC 
ACCATTTACG GAGATTTGGG AGACACGGGA GAGAGAAAAC TCAGCAAGTC CGAGACCAAC 
TCCAACAAGT TTGAGAGGGG ACAGGTGGAT AAATTCAATA TAGAAGCGGT GGATCTCGGC 
ACCATCTATA AGATTAGGAT CCGGCACGAT AACTCTATGT TGAACCCTGA CTGGTACCTG 
GAGAGGGTAG AGATCCTTAA TGAAGTCACG GAGGAGACGT ATCTGTTCAT CTGTGAGCGA 
TGGCTCTCCA CCAAGAAGGA AGACAAGAAG ATTGAACGAA TATTGTATGA GAAGGAGTAT 
GACGGGGAGC GACTCAGCTT GGGCAGCTCA TCCAAAAGTT TCTGGGGATC ATCCCTCAGC 
CTGAGAAGTC AGGATAACAA TGCCAATGTA AAGAGCAGGA ACAGCCTAGA TGGCTCCCAG 
CAAGATGGAA CATTAATCCC GTATCACATC ACTATCACCA CCGGCCTTGA CCGAGTTGGT 
GGGACAGAAA GTCGCGCTTA TGTCATCATA ATGGGACCTC AGAAGATAAG GACAGAGCGA 
CTTTGGCTGG ACCTGCCCAA AGATAAGAAG GGCTTTGCGC CAGGGTCTGT GGAGAAGTTC 
TCTGTGGTGG GATTGGATGT CGGAGAGATT AAAAAGATGG AGTTGGGCCA TGATGGGGTT 
ACCCCAGAAA GCTGCTGGCT GGTGGATGAG ATCCAACTAA CAGTGCCTAA CAAAGGAGTC 
ATGTATACCT TTGTCTGTAA ATGCTGGTTT GCCAAAGATA GAGGAGACGG TCTAACTGCC 
CGGGTCTTCA ATATGCTGGA TGCAACTGCC ATCACTATTG GCCATAAGAT TCTCTATGAG 
GTTACAGTTG TCACAGGAGA TGTACCAAAT GGGGGTACTG ATGCCAATAT TTATATTACC 
TGCTTTGGAG CAAGTGGGAG CTCAGAGGAA ATGCAGCTGC CAAAAGGGGA TGACAGGTTT 
GAGAGAGGCC AGCGTGACAC TTTTGTCATG GAGATCGCTG ACATTGCCCC ACTGAAGAAG 
ATGAGATTGC GTATCGATGG GAAGGGCAGC CGCCCTGAGT GGTATTTCGA ACAGATCTCC 
TTGAAGAACT TGACCACCGA AGAGGAGACA GTGTTCATGT ATGGGGACTG GATGTCCAAG 
ACCAAGGGGG AGAGGAAGTC GCTCTCCTGT GAAATGGCGG CCACAGTAGA CGGCGAAGAA 
ATGGTGGAAA CTACAACATA CATTATACAA GTCAAAACCA GCGACGTGTC AGGAGCCGGT 
ACCGACGCCA ACGTGTTCCT GATCATCTTT GGAGAGAACG GAGACACCGG CACTCTGGCG 
CTGAAGCAGT CCAACAATTC CAACAAGTTC GAAAGGAATC AGAGTGATGT CTTCAGATTC 
CCTGACATGT TGAGCCTCGG AGACCTGTGC AAAGTCCGGG TCTGGCACGA TAACTCAGGT 
ATTGGCCCCG GCTGGCACCT GGAATTTATC GACATAAAGG ACGAGACCAT GAGCAAGAGT 
TTCCGCTTCC AGTGCGACCG ATGGCTGGCC AAGAACGCCG ATGACCATCA GATCATCAGG 
GAACTCGCCT GTGCCAACAA CGATATCCTG GATCTGAAAG AGAAAACCTC TTATGAGATT 
GTGACGGTGA CAAGCGACAG GGATGATGCA GAGACCAAGG ACAACGTCTG GATCGTCTTG 
GAGGGCAAAA AAGGGAGGTC AAAAGAATTT CTAATGGAGA ACACTTCCAA AAAGCGGAGG 
TTTGCTCGGG ATGCAACCGA TACATTCAAG TTTTCCTCCA AGAACCTTGG TGACATCGCT 
GCCATCTGCG TTGGCCATTG CCCTAAGGAC GGAAGGAAGT TTTCTCCGAA GCCGGATTTA 
TACTGGCACG TCAAGGAGAT CATCGTGACC GAGACCGAAC TCGGAAACAA GTATGTGTTT 
AACTGCAACG CCAAGGTGCC GCTGCGCAGC AAGCGAGACG ACTACAGAGT GTTCGAGTGT 
GCCAAGGCCA TCGAGAGCTT CGCCAGCCGC GCCCGCAGTC TGGTGCCCGT CAAATACGAA 
GTCATCGTGG TCACCGGGTT CGAAAAGGGG GCGGGGACGG ACGCCAACGT GACCCTCGTC 
ATGTACGGCT CCAACGGGGA CTCCGGGAAG CACGCGCTGA AGCAGAAGAT GCGCAACCTC 
TTCGAGCGCG GGAAAACCAA CCGCTTCTAC ATAGAGACCC TCGACCTGGG GGAGATGAAG 
AAAGTGCGGG TGGAGCACGA TAACAGCGGC CTGAGCCCCG GCTGGCTACT CGAGCGGGTG 
GAGGTGACCA ACAGCGCCAC CGGCGTCACC ACCATATTCC CCTGCGGCAA GTGGCTGGAC 
AAGAAACGGG GCGACGGCGA GACCTGGAGG GAACTCTACC CCAAATACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031750797.1
CDS	179..7669
Translation	MPTQIQYYNSSPSVTGVLQPCTAPASFGQMRPRDAYPSTTAPRIRPATGFQFRDVSPPRGKPVWNDGFKSEGWRNSLRRYKVSLNHRPVESMLSKRAPYAGRTLSASSGGYRYRNGHTDTLPAYSAMNDPHLYDFYAKKFGLQVNPQPPSLKRAKSAPSRRSVLRSGSLSGKKVLYKIVVKTGDKKQAGTDARVFLQIKGSKGKLSKTQLYKASGTDSPDKAATFAFKKGSTNTFKVYGPEIGDIRTITIEHDGLEQRHAWFLEEVFVTNTDRDKSWYFPCRRWFSLYHTDCQLSRTLSPEIKPVSHELDGKKGDHTVYEVVTVTGDVRGAGTDANVFVTLFGEFGITPKIHLTSKSRTAFERSKTDVFRVKTNNVGPLKKIRIEHDNTGMNAGWYLDRAIITDMNRPHLRFYFPCNNWLSKEDGDGLYVRDLLGSLNPMDVPKMNKYVARVFTGAANGSGTDADVFLNIFGENGDTGERKLDNDKNNFEKGSEDKFTIDAPNLGKIRKITIGHNNKGGSAGWFLEKVIIEDIGNKTVYEFPCSRWLAIDEDDGKIQRDLLVGGSEATGIVYNVAIMTGDIRGAGTDSKIHVILHGEKGVKNSGKLFLEGGEFERGRIDIFNIEIAALLSPLSRVTIGHDGNGVGAGWYCEKVIVYCPFTGIEQTFPCGHWLDVDEGDGLIERELYEMLSLRQKRQKKHPWSLWIWTSDMKSAGTDANILLQVYGEKGKSDEIKLDNNSDNFEAGQVDRFMIELPDLGTLYKIRVWHEKRAAFSGWHLNKVTLLKTLTKEKYTFKCGRWLDLNEDDHEIVRELPGEGPLIDSVLPVVKYRVTVYTGTVSGSGTDANVFICLFGELGDTGERFLIECKNNMNKFEKGNADDFIIEAVTLKQVKRVRIGHDGKGGGCGWYMDKVVVREDGKPETEAIEFPCNRWFDRNEDDGHIIRELVPAGDSQNLRSISYHFQIKTGDVSGASSDSRVFVKLYGEKGDTNKQFLLVSDNDLADNFERGRVDNFTINTIDIGKINKLLIGHDNVGIRAGWFLANVVVQVPAHGKEYMFPVNRWLCKDECDGKVEVEVYASEVKSIEKMVNYEVVVTTGNVRGSGTNANVFMQLYGEQGKTEETFLRSRSNDFERGAVDIFKIEAAEVGKIRKLRIGHDGSGFGDGWYLESVEIKRVGIKMMPVEKPEKKKKPNKKKKSSKEEEEELQPREVVESYKFECKRWLARGEEDGELVVELLPLETSEIEENSYEVHVFTGTVWGAGTDANVYAAIYGELGDTGERQLKKANHLNKFERGQEDIFTIKAVDLGELKKLKIRHDNKGANAGWFLDRVEIVDEKDDTKYFFPCQRWLAVDEDDGQIARQLVPVDEAFMKKEDEGQDQSQATLGLEQKAKSTTYTVTIKTGDKKNSGTDANVFLILYGEKDDTGFMNLKASKTNKNKFERGQTDVFTVEAVDIGTLAKLKIGHDNKGTSAGWFLDWVEIDAPSLGQRVKFPCGRWLDKSEDDGTIERYLFPSELQTEQYVPFVPYEITVYTSDIFAAGTDADVFIVLYGRDGVCTQQKSLCVNKRERRLYFERNSVDQFIVELEDVGDIIEKIRIGHTDKGMSAGWHLDRVEIRRLLPNGKGSQTVTFPCERWLAKSEDDGETVRELVPSDIFTEKLMKDGTLKQIEMEVEDPLETHTYSISVFTGDIYGSGTDANVFITIYGDLGDTGERKLSKSETNSNKFERGQVDKFNIEAVDLGTIYKIRIRHDNSMLNPDWYLERVEILNEVTEETYLFICERWLSTKKEDKKIERILYEKEYDGERLSLGSSSKSFWGSSLSLRSQDNNANVKSRNSLDGSQQDGTLIPYHITITTGLDRVGGTESRAYVIIMGPQKIRTERLWLDLPKDKKGFAPGSVEKFSVVGLDVGEIKKMELGHDGVTPESCWLVDEIQLTVPNKGVMYTFVCKCWFAKDRGDGLTARVFNMLDATAITIGHKILYEVTVVTGDVPNGGTDANIYITCFGASGSSEEMQLPKGDDRFERGQRDTFVMEIADIAPLKKMRLRIDGKGSRPEWYFEQISLKNLTTEEETVFMYGDWMSKTKGERKSLSCEMAATVDGEEMVETTTYIIQVKTSDVSGAGTDANVFLIIFGENGDTGTLALKQSNNSNKFERNQSDVFRFPDMLSLGDLCKVRVWHDNSGIGPGWHLEFIDIKDETMSKSFRFQCDRWLAKNADDHQIIRELACANNDILDLKEKTSYEIVTVTSDRDDAETKDNVWIVLEGKKGRSKEFLMENTSKKRRFARDATDTFKFSSKNLGDIAAICVGHCPKDGRKFSPKPDLYWHVKEIIVTETELGNKYVFNCNAKVPLRSKRDDYRVFECAKAIESFASRARSLVPVKYEVIVVTGFEKGAGTDANVTLVMYGSNGDSGKHALKQKMRNLFERGKTNRFYIETLDLGEMKKVRVEHDNSGLSPGWLLERVEVTNSATGVTTIFPCGKWLDKKRGDGETWRELYPKY

Target ORF information:

RefSeq Version	XM_031894937.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis lipoxygenase homology domains 1 (loxhd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031894937.1

ORF Insert Sequence:

ATGCCGACCC AGATCCAGTA TTACAACTCG TCCCCGTCAG TCACGGGTGT GCTCCAGCCT 
TGCACTGCGC CCGCTTCTTT TGGGCAGATG AGACCAAGGG ATGCCTATCC CAGTACCACG 
GCACCTCGGA TCCGACCAGC CACTGGGTTC CAGTTCCGAG ATGTCTCCCC TCCCCGGGGG 
AAGCCTGTGT GGAACGACGG ATTCAAGTCA GAGGGCTGGA GAAACTCCCT GCGCAGATAT 
AAAGTCAGTC TGAACCACAG GCCCGTGGAG TCCATGCTAA GCAAACGAGC TCCATATGCA 
GGAAGAACTC TAAGTGCCAG CTCCGGCGGG TATCGGTACA GAAACGGGCA CACAGACACT 
CTGCCGGCGT ATAGCGCTAT GAACGACCCT CATTTATATG ATTTCTATGC CAAGAAGTTT 
GGATTGCAGG TGAACCCCCA GCCGCCCTCA CTGAAACGGG CCAAGAGCGC CCCCTCCAGA 
AGATCTGTGC TCCGCTCCGG TTCTCTCTCT GGGAAGAAGG TTCTGTACAA GATTGTGGTG 
AAGACTGGAG ACAAGAAGCA GGCCGGCACT GACGCAAGGG TATTTCTCCA GATAAAGGGA 
TCCAAAGGAA AGCTCAGTAA GACCCAGCTG TATAAAGCAT CTGGAACAGA CTCACCCGAC 
AAGGCAGCAA CTTTTGCATT CAAAAAGGGC TCCACCAACA CATTCAAAGT GTACGGCCCC 
GAAATCGGAG ACATCAGGAC GATCACAATT GAGCACGATG GCCTGGAGCA GCGGCACGCT 
TGGTTCCTGG AGGAAGTTTT TGTAACCAAT ACTGATCGGG ACAAATCCTG GTACTTCCCA 
TGCCGGAGAT GGTTCTCCCT GTACCACACA GACTGCCAGC TGAGCCGCAC TTTATCCCCG 
GAGATTAAAC CCGTATCTCA TGAACTTGAT GGCAAAAAAG GGGACCACAC TGTATACGAG 
GTGGTCACTG TTACAGGAGA CGTCCGGGGG GCCGGCACCG ACGCCAATGT GTTTGTCACC 
CTTTTCGGAG AATTCGGAAT CACTCCCAAG ATTCACCTCA CCAGCAAGAG TAGGACTGCC 
TTCGAACGGT CAAAGACTGA TGTGTTTAGA GTCAAAACCA ATAACGTCGG ACCACTCAAG 
AAGATCAGAA TTGAGCATGA TAACACAGGA ATGAATGCAG GATGGTATCT GGATCGGGCC 
ATCATAACGG ATATGAACAG GCCCCATCTC CGGTTCTACT TCCCCTGCAA CAACTGGCTG 
AGCAAGGAAG ATGGCGATGG GCTTTACGTC AGGGATCTGC TGGGCAGTCT CAATCCCATG 
GATGTGCCCA AAATGAACAA ATATGTTGCA AGAGTCTTTA CTGGTGCAGC CAATGGCAGT 
GGGACTGATG CAGATGTTTT CCTCAATATT TTTGGGGAGA ATGGAGACAC AGGGGAGCGG 
AAACTTGACA ATGATAAAAA CAACTTTGAG AAGGGGTCAG AAGACAAATT TACTATTGAT 
GCGCCAAATC TGGGGAAAAT AAGGAAAATC ACTATAGGTC ACAACAACAA AGGAGGCTCG 
GCAGGCTGGT TCCTGGAGAA GGTTATCATT GAAGATATTG GAAACAAAAC CGTTTATGAG 
TTCCCCTGCT CTCGCTGGCT CGCTATCGAT GAAGATGATG GAAAGATTCA GCGAGATCTG 
CTTGTCGGAG GGAGTGAAGC AACAGGCATT GTCTACAACG TTGCAATTAT GACTGGAGAT 
ATCCGAGGTG CCGGAACAGA TTCCAAGATC CACGTTATCC TCCATGGAGA GAAAGGTGTG 
AAGAACAGCG GCAAACTCTT CTTGGAAGGA GGGGAATTCG AGAGAGGGAG GATAGACATT 
TTCAACATTG AAATCGCAGC GCTACTGAGC CCCCTCAGCA GAGTGACAAT TGGACATGAT 
GGAAACGGTG TAGGGGCTGG ATGGTACTGT GAGAAGGTTA TAGTTTACTG CCCATTCACC 
GGCATTGAAC AAACATTTCC ATGTGGCCAT TGGTTGGATG TAGACGAAGG AGATGGCTTA 
ATAGAGAGAG AACTCTATGA AATGTTGTCC TTACGCCAGA AGAGGCAGAA GAAACATCCA 
TGGTCCCTTT GGATATGGAC GAGCGATATG AAAAGTGCCG GAACCGATGC CAACATCCTT 
CTTCAGGTCT ACGGAGAGAA AGGAAAGTCT GATGAAATAA AACTGGACAA TAACTCCGAC 
AACTTTGAAG CTGGACAGGT CGACAGGTTC ATGATTGAAC TTCCTGATCT GGGAACTCTG 
TACAAAATCC GAGTTTGGCA CGAGAAGAGA GCGGCCTTTT CCGGTTGGCA TTTAAACAAG 
GTAACCCTGC TGAAGACACT TACCAAAGAG AAATATACCT TTAAATGCGG TCGCTGGTTG 
GACCTGAATG AAGACGACCA TGAAATTGTA AGAGAGCTTC CAGGAGAGGG GCCACTTATA 
GACTCCGTCC TGCCTGTGGT AAAGTATCGC GTGACTGTGT ACACTGGTAC CGTTAGCGGG 
AGCGGCACCG ATGCCAATGT GTTTATCTGT CTCTTTGGTG AACTCGGCGA CACAGGAGAG 
CGTTTTCTCA TCGAATGCAA AAACAATATG AATAAGTTTG AGAAAGGCAA TGCTGATGAC 
TTTATTATTG AAGCGGTGAC CCTGAAGCAG GTGAAAAGAG TGCGGATCGG ACACGATGGG 
AAAGGAGGAG GCTGTGGTTG GTATATGGAC AAAGTTGTAG TGAGAGAAGA TGGGAAACCG 
GAGACAGAGG CTATTGAATT TCCTTGCAAC AGGTGGTTTG ACAGAAATGA GGATGACGGT 
CACATCATTA GGGAGCTGGT TCCCGCCGGA GACTCTCAGA ATCTGAGGAG TATCAGCTAT 
CACTTTCAGA TCAAAACGGG GGACGTCAGC GGAGCCAGCT CAGATTCCAG GGTCTTCGTC 
AAGCTCTACG GAGAGAAGGG GGACACCAAC AAGCAGTTCC TGCTGGTGTC CGATAACGAC 
CTGGCGGATA ACTTTGAGAG GGGCCGAGTG GATAATTTCA CCATAAATAC CATCGATATA 
GGGAAGATCA ATAAGCTGTT GATTGGCCAT GACAATGTAG GGATTCGAGC GGGTTGGTTC 
CTGGCCAACG TTGTGGTTCA GGTCCCCGCC CATGGCAAGG AGTACATGTT TCCCGTTAAC 
CGCTGGCTGT GCAAAGACGA GTGCGACGGC AAGGTGGAGG TGGAAGTGTA CGCTAGCGAG 
GTGAAAAGCA TTGAGAAAAT GGTAAATTAT GAAGTGGTAG TAACCACCGG CAACGTACGT 
GGATCTGGAA CCAACGCCAA CGTCTTCATG CAACTCTACG GGGAACAGGG GAAGACGGAG 
GAAACGTTTT TAAGAAGCCG TTCTAATGAC TTTGAAAGAG GAGCTGTAGA TATATTTAAG 
ATCGAGGCCG CAGAAGTGGG TAAGATCCGC AAGCTCAGGA TTGGCCACGA TGGCAGCGGC 
TTTGGAGACG GCTGGTATTT GGAGAGCGTT GAGATCAAGC GAGTCGGAAT AAAGATGATG 
CCTGTGGAGA AGCCGGAGAA GAAGAAAAAA CCCAACAAGA AGAAGAAATC CAGTAAGGAG 
GAAGAAGAGG AGCTTCAGCC TCGAGAAGTG GTGGAGTCCT ACAAGTTTGA GTGCAAGCGC 
TGGTTGGCCA GGGGGGAAGA AGATGGAGAG CTAGTGGTTG AACTTCTGCC TCTGGAGACT 
TCAGAAATAG AAGAGAACAG CTACGAGGTC CATGTATTTA CCGGTACCGT TTGGGGAGCT 
GGAACAGACG CCAATGTTTA TGCCGCCATA TATGGAGAAT TAGGAGACAC GGGAGAACGT 
CAGCTAAAGA AAGCCAACCA TCTGAACAAA TTTGAGAGGG GCCAGGAGGA TATATTCACT 
ATTAAAGCTG TAGATTTAGG AGAGCTTAAG AAGCTGAAAA TCCGCCACGA CAACAAGGGT 
GCCAACGCTG GGTGGTTCCT CGACAGAGTA GAGATTGTGG ATGAGAAGGA CGACACAAAG 
TATTTCTTCC CATGCCAGAG ATGGCTTGCT GTCGATGAAG ACGACGGACA GATCGCCAGG 
CAGCTGGTCC CTGTGGACGA GGCCTTTATG AAGAAAGAAG ACGAAGGGCA GGACCAGTCT 
CAAGCCACAC TGGGTTTGGA ACAGAAAGCC AAATCGACAA CTTACACAGT GACAATAAAG 
ACCGGAGACA AGAAGAATTC TGGGACAGAT GCCAATGTAT TCTTAATCTT GTATGGAGAG 
AAAGATGATA CAGGGTTCAT GAACCTCAAG GCTTCCAAGA CCAACAAGAA TAAGTTTGAG 
CGAGGCCAAA CTGATGTGTT TACCGTGGAG GCCGTGGATA TCGGGACTCT CGCCAAACTA 
AAAATTGGGC ACGATAACAA AGGCACCTCG GCTGGTTGGT TCCTGGACTG GGTAGAGATT 
GATGCACCAT CACTTGGTCA GCGGGTTAAG TTCCCCTGTG GTCGCTGGTT GGATAAAAGT 
GAAGATGATG GGACCATCGA AAGATATCTG TTCCCATCTG AACTGCAAAC TGAGCAGTAT 
GTTCCATTTG TTCCGTACGA GATCACAGTC TACACCAGCG ATATATTCGC GGCCGGCACG 
GACGCCGACG TCTTTATCGT TCTGTATGGC CGAGACGGAG TCTGTACCCA GCAGAAATCC 
CTGTGTGTGA ACAAGAGAGA GCGGAGATTG TACTTTGAGC GAAACTCGGT GGATCAGTTC 
ATTGTAGAGC TAGAAGACGT TGGAGATATC ATTGAGAAGA TTCGCATTGG GCATACTGAC 
AAAGGCATGA GCGCAGGGTG GCATTTAGAC AGAGTGGAAA TTCGTAGACT GCTGCCAAAT 
GGAAAGGGCT CCCAGACCGT AACCTTTCCG TGTGAGCGGT GGCTTGCCAA ATCCGAGGAT 
GACGGAGAGA CAGTGAGAGA ACTAGTGCCA TCCGATATCT TTACTGAGAA ACTCATGAAA 
GATGGGACCT TGAAACAGAT TGAGATGGAA GTAGAAGACC CTCTGGAAAC CCACACGTAC 
AGCATATCTG TGTTCACCGG CGACATCTAT GGTTCGGGTA CCGACGCCAA CGTCTTCATC 
ACCATTTACG GAGATTTGGG AGACACGGGA GAGAGAAAAC TCAGCAAGTC CGAGACCAAC 
TCCAACAAGT TTGAGAGGGG ACAGGTGGAT AAATTCAATA TAGAAGCGGT GGATCTCGGC 
ACCATCTATA AGATTAGGAT CCGGCACGAT AACTCTATGT TGAACCCTGA CTGGTACCTG 
GAGAGGGTAG AGATCCTTAA TGAAGTCACG GAGGAGACGT ATCTGTTCAT CTGTGAGCGA 
TGGCTCTCCA CCAAGAAGGA AGACAAGAAG ATTGAACGAA TATTGTATGA GAAGGAGTAT 
GACGGGGAGC GACTCAGCTT GGGCAGCTCA TCCAAAAGTT TCTGGGGATC ATCCCTCAGC 
CTGAGAAGTC AGGATAACAA TGCCAATGTA AAGAGCAGGA ACAGCCTAGA TGGCTCCCAG 
CAAGATGGAA CATTAATCCC GTATCACATC ACTATCACCA CCGGCCTTGA CCGAGTTGGT 
GGGACAGAAA GTCGCGCTTA TGTCATCATA ATGGGACCTC AGAAGATAAG GACAGAGCGA 
CTTTGGCTGG ACCTGCCCAA AGATAAGAAG GGCTTTGCGC CAGGGTCTGT GGAGAAGTTC 
TCTGTGGTGG GATTGGATGT CGGAGAGATT AAAAAGATGG AGTTGGGCCA TGATGGGGTT 
ACCCCAGAAA GCTGCTGGCT GGTGGATGAG ATCCAACTAA CAGTGCCTAA CAAAGGAGTC 
ATGTATACCT TTGTCTGTAA ATGCTGGTTT GCCAAAGATA GAGGAGACGG TCTAACTGCC 
CGGGTCTTCA ATATGCTGGA TGCAACTGCC ATCACTATTG GCCATAAGAT TCTCTATGAG 
GTTACAGTTG TCACAGGAGA TGTACCAAAT GGGGGTACTG ATGCCAATAT TTATATTACC 
TGCTTTGGAG CAAGTGGGAG CTCAGAGGAA ATGCAGCTGC CAAAAGGGGA TGACAGGTTT 
GAGAGAGGCC AGCGTGACAC TTTTGTCATG GAGATCGCTG ACATTGCCCC ACTGAAGAAG 
ATGAGATTGC GTATCGATGG GAAGGGCAGC CGCCCTGAGT GGTATTTCGA ACAGATCTCC 
TTGAAGAACT TGACCACCGA AGAGGAGACA GTGTTCATGT ATGGGGACTG GATGTCCAAG 
ACCAAGGGGG AGAGGAAGTC GCTCTCCTGT GAAATGGCGG CCACAGTAGA CGGCGAAGAA 
ATGGTGGAAA CTACAACATA CATTATACAA GTCAAAACCA GCGACGTGTC AGGAGCCGGT 
ACCGACGCCA ACGTGTTCCT GATCATCTTT GGAGAGAACG GAGACACCGG CACTCTGGCG 
CTGAAGCAGT CCAACAATTC CAACAAGTTC GAAAGGAATC AGAGTGATGT CTTCAGATTC 
CCTGACATGT TGAGCCTCGG AGACCTGTGC AAAGTCCGGG TCTGGCACGA TAACTCAGGT 
ATTGGCCCCG GCTGGCACCT GGAATTTATC GACATAAAGG ACGAGACCAT GAGCAAGAGT 
TTCCGCTTCC AGTGCGACCG ATGGCTGGCC AAGAACGCCG ATGACCATCA GATCATCAGG 
GAACTCGCCT GTGCCAACAA CGATATCCTG GATCTGAAAG AGAAAACCTC TTATGAGATT 
GTGACGGTGA CAAGCGACAG GGATGATGCA GAGACCAAGG ACAACGTCTG GATCGTCTTG 
GAGGGCAAAA AAGGGAGGTC AAAAGAATTT CTAATGGAGA ACACTTCCAA AAAGCGGAGG 
TTTGCTCGGG ATGCAACCGA TACATTCAAG TTTTCCTCCA AGAACCTTGG TGACATCGCT 
GCCATCTGCG TTGGCCATTG CCCTAAGGAC GGAAGGAAGT TTTCTCCGAA GCCGGATTTA 
TACTGGCACG TCAAGGAGAT CATCGTGACC GAGACCGAAC TCGGAAACAA GTATGTGTTT 
AACTGCAACG CCAAGGTGCC GCTGCGCAGC AAGCGAGACG ACTACAGAGT GTTCGAGTGT 
GCCAAGGCCA TCGAGAGCTT CGCCAGCCGC GCCCGCAGTC TGGTGCCCGT CAAATACGAA 
GTCATCGTGG TCACCGGGTT CGAAAAGGGG GCGGGGACGG ACGCCAACGT GACCCTCGTC 
ATGTACGGCT CCAACGGGGA CTCCGGGAAG CACGCGCTGA AGCAGAAGAT GCGCAACCTC 
TTCGAGCGCG GGAAAACCAA CCGCTTCTAC ATAGAGACCC TCGACCTGGG GGAGATGAAG 
AAAGTGCGGG TGGAGCACGA TAACAGCGGC CTGAGCCCCG GCTGGCTACT CGAGCGGGTG 
GAGGTGACCA ACAGCGCCAC CGGCGTCACC ACCATATTCC CCTGCGGCAA GTGGCTGGAC 
AAGAAACGGG GCGACGGCGA GACCTGGAGG GAACTCTACC CCAAATACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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loxhd1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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