map4k4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following map4k4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the map4k4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa05830 XM_002934506.5
Latest version!
Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 4 (map4k4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa05831 XM_004911783.4
Latest version!
Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 4 (map4k4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa05832 XM_004911784.4
Latest version!
Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 4 (map4k4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa05833 XM_004911785.4
Latest version!
Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 4 (map4k4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa35138 XM_012957670.3
Latest version!
Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 4 (map4k4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa35139 XM_012957671.3
Latest version!
Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 4 (map4k4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa35140 XM_012957672.3
Latest version!
Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 4 (map4k4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa35141 XM_012957673.3
Latest version!
Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 4 (map4k4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa42793 XM_018091631.1
Latest version!
Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 4 (map4k4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OXa42794 XM_018091632.1
Latest version!
Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 4 (map4k4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OXa42795 XM_018091633.1
Latest version!
Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 4 (map4k4), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OXa42796 XM_018091634.1
Latest version!
Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 4 (map4k4), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OXa42797 XM_018091635.1
Latest version!
Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 4 (map4k4), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
View Old Accession Versions >>
OXa35138 XM_012957670.2 Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 4 (map4k4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa35140 XM_012957672.2 Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 4 (map4k4), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa05830 XM_002934506.4 Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 4 (map4k4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa35139 XM_012957671.2 Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 4 (map4k4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa05831 XM_004911783.3 Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 4 (map4k4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa05832 XM_004911784.3 Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 4 (map4k4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa05833 XM_004911785.3 Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 4 (map4k4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa35141 XM_012957673.2 Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 4 (map4k4), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa05830
    Clone ID Related Accession (Same CDS sequence) XM_002934506.4 , XM_002934506.5
    Accession Version XM_002934506.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4212bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002934506.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCCAACG ATTCGCCTGC CAAAAGTCTT GTGGATATAG ACCTGTCGTC TCTGAGGGAC 
    CCAGCTGGCA TCTTTGAATT AGTGGAAGTG GTAGGAAATG GCACCTATGG ACAAGTATAT
    AAGGGCCGAC ATGTCAAAAC AGGCCAACTT GCAGCCATCA AAGTCATGGA TGTTACAGAG
    GATGAAGAGG AAGAAATCAA ACTGGAAATC AATATGTTGA AGAAATATTC CCACCACAGA
    AATATAGCTA CTTATTATGG TGCTTTTATT AAGAAAAGTC CTCCTGGGCA TGATGACCAG
    CTTTGGTTGG TGATGGAGTT TTGTGGTGCT GGCTCAATCA CTGACCTGGT GAAGAATACA
    AAGGGGAATA GTTTGAAAGA AGACTGGATT GCCTATATCT CTCGTGAGAT ACTTAGGGGA
    TTGGCCCACC TCCATGCTCA TCATGTGATT CACCGGGATA TCAAAGGACA GAATGTGCTG
    CTAACAGAGA ATGCCGAAGT GAAATTAGTG GATTTTGGTG TTAGTGCACA GCTGGATAGG
    ACAGTGGGCC GAAGGAACAC ATTTATTGGT ACACCATACT GGATGGCTCC AGAAGTTATC
    GCCTGTGATG AGAACCCAGA TGCTACCTAT GACTATAGGA GTGACCTCTG GTCATGTGGT
    ATTACAGCGA TTGAAATGGC AGAAGGTGCT CCTCCTCTTT GCGATATGCA TCCAATGCGA
    GCCCTGTTCC TTATCCCAAG AAATCCTCCA CCACGGCTAA AATCCAAGAA ATGGTCAAAG
    AAGTTCTTCA GCTTTATAGA AGGCTGCCTT GTGAAAAATT ACATGCAACG TCCCCCAACA
    GAACAGCTTT TAAAGCATCC ATTTATACGA GATCAGCCAA ATGAAAGGCA AGTCCGAATT
    CAGCTGAAGG ATCACATAGA CAGGACAAGG AAGAAGAGAG GAGAAAAAGA TGAAACAGAG
    TATGAATACA GTGGAAGTGA AGAGGAGGAA GAGGAAGTGC CTGAACAGGA AGGAGAACCA
    AGTTCCATTG TTAATGTGCC TGGGGAATCA ACACTCCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGCTC TGAGGCTTTG CGACGGCAAC AGCTGCTGCA GGAGCAACAA
    CTCAGAGAGC AGGAGGAATA TAAAAGACAG CTCCTGGCAG AGAGACAGAA GCGCATAGAA
    CAGCAGAAAG AGCAAAGGAG GAGACTTGAA GAGCAACAAA GAAGAGAGCG TGAGGCTCGT
    CGGCAGCAGG AACGAGAACA AAGAAGGCGA GAGCAAGAGG AAAAGAAGAG AATGGAGGAG
    ATGGAAAGAA GGCGCAAGGA AGAGGAAGAA AGGAGAAGAG CAGAGGAAGA GAAAAGAAGA
    GTGGAAAGAG AGCAGGAGTA TATCCGAAGA CAGCTGGAGG AGGAGCAGCG GCACTTAGAA
    ATCCTTCAGC AGCAGCTGCT TCAGGAGCAG GCCATGTTAC TGGAGTATAG ATGGCGTGAA
    ATGGAGGAGC ACCGCCAGGC TGAAAGACTT CAGCGTCAGT TGCAGCAAGA GCAGGCCTAT
    CTCATGTCAC TACAGCATGA TCATAGACAG CAGCAGCCAC CACAACAGCA GCCCCAGCCC
    CAGCAGCCTC CACTGTCCCA GCAGGAAAGG AGCAAGCAAA CATACCATAC CCAAGAACCC
    AAGCCTCATT ATGAACAAGC AGAACGAATA CGAGAGGCAG AGGAGCGCCT TAGGAAAGCA
    AACCAAGGCT CCCCTGAAGC CCAGGCTAAG CAACCAGGAA GAGTTTTAGA GCCATTAGTG
    CCTTCAAGAT CCGAATCATT TTCCAATGGA AATTCTGAGC CTGCTCAACC TGCCATGCAC
    AGACCAGTGG AACCACAGGT ACAGTGGTCT CATCTGGCAT CCCTCAAGAA CAATGTATCC
    CCTGTCTCGC GATCCCATTC CTTCAGTGAC CCTTCTCCCA AATTTGCACC TCACCTTCTA
    CGATCTCAGG ATTCGCACCA AACTTCACGC AGTGAAGTGC AAAGTCAGGG TTCTGACCCT
    AAGTCTGAAA TCTCTGAGCC AACCCAAAGG GTTTGGGCTC GATCAGACAG TGACGAGGTG
    CCTCCAAGGG TGCCTGTGAG GACAACATCA AGATCTCCAG TATTGTCACG GAAAGATTCA
    CCACTGCAGG GCAGTGGGCA GCAAAATACC CAAGCTGGTC AGAGAAATTC CACAAGCAAT
    ATTGAGCCTA GACTTCTTTG GGAAAGAGTA GAGAAGCTTG TGCCCAGACC GGGTAGTGGT
    AGCTCGTCAG GATCAAGTAA CTCTGGATCC CAAGCAGGAT CCCACCCAGG ATCTCAGAGT
    GGATCTGGAG AGCGCTTCCG GATGAGATCA TCTTCAAAAT CTGAGGGCTC ACCATCTCAA
    CGACATGAAA ACACAGCCAA AAAGTCTGAA GAGAAGAAAG AAGCAGTTAG ACAGGCCAAA
    GCTCCTGGAG AAGTGGATCT TACTGCACTC GCTAAGGAGC TTCGAGCAGT AGATGATGTT
    CGGCCACTTC ACAAAGTTAC TGACTATTCC TCATCCAGTG AAGAGTCTGG TACTACAGAT
    GAGGAGGATG ATGACATGGA ACAGGAAGGA GCAGATGAAT CGACTTCAGG CCCAGAGGAT
    GCTAGAGCTA TGTCATCTTT GAATTTAAGC AATGGAGAAA CGGAGTCTGT GAAAACCATG
    ATCGTACATG ATGATGTCGA AAGTGAACCT GCCATGACCC CATCTAAAGA AGGCACTTTA
    ATTGTCCGAC AGAGTACAGT TGACAAAAAG CGTGCCAACC AACTTGAGAG CAATGGCTTT
    GCGAGTCGTA TTCACCTCTT GCCAGATCTC TTACAGCAAA GCCATTCCTC CTCCACCACT
    TCTTCCCCAA CCTCAAGTCA ACCGACACCT ACCATGTCCC CCCAGACACC CCAGGACAAG
    CTAACTGCTA ATGAGACTCA GTCAGCTAGT AATACTCTCC AGAAACACAA ATCGTCCTCC
    TCTTTCACAC CGTTCATAGA TCCAAGGTTA CTTCAGATCT CTCCATCTAG TGGAACAACT
    GTTACGTCAG TAGGTACATA CATGCGCACA CTGGAAGCTA ACCTTAAAAA TACTAATTTT
    ATTTTAGTGG GATTTTCCAG TGAAGCGCTA AGGCCGGATG CCATGAGGCA AGATCCTACA
    AGGAAAGGAT CAGTTGTTAA TGTTAATCCC ACAAATACTC GGCCACAAAG TGATACACCA
    GAGATTCGCA AATACAAGAA GAGATTTAAT TCTGAGATTT TGTGTGCGGC ATTGTGGGGT
    GTAAATTTGC TGGTGGGAAC TGAAAGTGGT TTAATGCTGT TGGATAGAAG TGGACAAGGG
    AAAGTGTACC CCCTCATTAA CAGAAGGCGA TTTCAGCAGA TGGATGTGCT TGAAGGCTTG
    AATGTCTTGG TAACTATATC AGGGAAAAAG AACAAGCTGA GAGTATATTA CTTATCCTGG
    CTGAGGAATA AAATTTTGCA CAATGATCCT GAAGTAGAGA AGAAACAAGG ATGGACAACA
    GTTGGGGATT TGGAAGGCTG TGTGCACTAC AAAGTTGTAA AATATGAACG TATCAAGTTT
    TTGGTAATCG CTTTAAAAAG TTCAGTGGAG GTGTATGCCT GGGCACCGAA ACCATACCAC
    AAGTTTATGG CCTTTAAGTC ATTCGGAGAT TTAGTACACA GACCTCTCCT TGTAGACCTG
    ACTGTGGAAG AAGGACAGAG GCTGAAAGTG ATCTATGGTT CCTGTGCTGG GTTTCATGCA
    GTAGATGTGG ACTCGGGCGC AGTTTATGAT ATTTACTTGC CAACACATAT TCAGAGCAGC
    ATTCAGCCAC ACGCAATCAT CATTCTCCCA AATACTGATG GTATGGAACT GCTGGTTTGC
    TATGAAGATG AAGGAGTTTA TGTTAATACA TACGGCAGGA TCACCAAAGA TGTAGTTCTA
    CAATGGGGAG AAATGCCTAC ATCAGTTGCT TATATCCGAT CTAACCAGAT CATGGGATGG
    GGTGAAAAAG CTATTGAGAT TCGATCCGTG GAAACTGGCC ACCTTGATGG AGTATTTATG
    CACAAAAGGG CACAAAGGCT CAAGTTCTTG TGCGAACGAA ATGACAAGGT TTTCTTTGCC
    TCGGTGCGGT CTGGCGGAAG CAGCCAAGTG TATTTCATGA CTTTGGGCAG AAGCTCTCTT
    CTGAGCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002934552.1
    CDS344..4555
    Translation

    Target ORF information:

    RefSeq Version XM_002934506.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 4 (map4k4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002934506.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCCAACG ATTCGCCTGC CAAAAGTCTT GTGGATATAG ACCTGTCGTC TCTGAGGGAC 
    CCAGCTGGCA TCTTTGAATT AGTGGAAGTG GTAGGAAATG GCACCTATGG ACAAGTATAT
    AAGGGCCGAC ATGTCAAAAC AGGCCAACTT GCAGCCATCA AAGTCATGGA TGTTACAGAG
    GATGAAGAGG AAGAAATCAA ACTGGAAATC AATATGTTGA AGAAATATTC CCACCACAGA
    AATATAGCTA CTTATTATGG TGCTTTTATT AAGAAAAGTC CTCCTGGGCA TGATGACCAG
    CTTTGGTTGG TGATGGAGTT TTGTGGTGCT GGCTCAATCA CTGACCTGGT GAAGAATACA
    AAGGGGAATA GTTTGAAAGA AGACTGGATT GCCTATATCT CTCGTGAGAT ACTTAGGGGA
    TTGGCCCACC TCCATGCTCA TCATGTGATT CACCGGGATA TCAAAGGACA GAATGTGCTG
    CTAACAGAGA ATGCCGAAGT GAAATTAGTG GATTTTGGTG TTAGTGCACA GCTGGATAGG
    ACAGTGGGCC GAAGGAACAC ATTTATTGGT ACACCATACT GGATGGCTCC AGAAGTTATC
    GCCTGTGATG AGAACCCAGA TGCTACCTAT GACTATAGGA GTGACCTCTG GTCATGTGGT
    ATTACAGCGA TTGAAATGGC AGAAGGTGCT CCTCCTCTTT GCGATATGCA TCCAATGCGA
    GCCCTGTTCC TTATCCCAAG AAATCCTCCA CCACGGCTAA AATCCAAGAA ATGGTCAAAG
    AAGTTCTTCA GCTTTATAGA AGGCTGCCTT GTGAAAAATT ACATGCAACG TCCCCCAACA
    GAACAGCTTT TAAAGCATCC ATTTATACGA GATCAGCCAA ATGAAAGGCA AGTCCGAATT
    CAGCTGAAGG ATCACATAGA CAGGACAAGG AAGAAGAGAG GAGAAAAAGA TGAAACAGAG
    TATGAATACA GTGGAAGTGA AGAGGAGGAA GAGGAAGTGC CTGAACAGGA AGGAGAACCA
    AGTTCCATTG TTAATGTGCC TGGGGAATCA ACACTCCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGCTC TGAGGCTTTG CGACGGCAAC AGCTGCTGCA GGAGCAACAA
    CTCAGAGAGC AGGAGGAATA TAAAAGACAG CTCCTGGCAG AGAGACAGAA GCGCATAGAA
    CAGCAGAAAG AGCAAAGGAG GAGACTTGAA GAGCAACAAA GAAGAGAGCG TGAGGCTCGT
    CGGCAGCAGG AACGAGAACA AAGAAGGCGA GAGCAAGAGG AAAAGAAGAG AATGGAGGAG
    ATGGAAAGAA GGCGCAAGGA AGAGGAAGAA AGGAGAAGAG CAGAGGAAGA GAAAAGAAGA
    GTGGAAAGAG AGCAGGAGTA TATCCGAAGA CAGCTGGAGG AGGAGCAGCG GCACTTAGAA
    ATCCTTCAGC AGCAGCTGCT TCAGGAGCAG GCCATGTTAC TGGAGTATAG ATGGCGTGAA
    ATGGAGGAGC ACCGCCAGGC TGAAAGACTT CAGCGTCAGT TGCAGCAAGA GCAGGCCTAT
    CTCATGTCAC TACAGCATGA TCATAGACAG CAGCAGCCAC CACAACAGCA GCCCCAGCCC
    CAGCAGCCTC CACTGTCCCA GCAGGAAAGG AGCAAGCAAA CATACCATAC CCAAGAACCC
    AAGCCTCATT ATGAACAAGC AGAACGAATA CGAGAGGCAG AGGAGCGCCT TAGGAAAGCA
    AACCAAGGCT CCCCTGAAGC CCAGGCTAAG CAACCAGGAA GAGTTTTAGA GCCATTAGTG
    CCTTCAAGAT CCGAATCATT TTCCAATGGA AATTCTGAGC CTGCTCAACC TGCCATGCAC
    AGACCAGTGG AACCACAGGT ACAGTGGTCT CATCTGGCAT CCCTCAAGAA CAATGTATCC
    CCTGTCTCGC GATCCCATTC CTTCAGTGAC CCTTCTCCCA AATTTGCACC TCACCTTCTA
    CGATCTCAGG ATTCGCACCA AACTTCACGC AGTGAAGTGC AAAGTCAGGG TTCTGACCCT
    AAGTCTGAAA TCTCTGAGCC AACCCAAAGG GTTTGGGCTC GATCAGACAG TGACGAGGTG
    CCTCCAAGGG TGCCTGTGAG GACAACATCA AGATCTCCAG TATTGTCACG GAAAGATTCA
    CCACTGCAGG GCAGTGGGCA GCAAAATACC CAAGCTGGTC AGAGAAATTC CACAAGCAAT
    ATTGAGCCTA GACTTCTTTG GGAAAGAGTA GAGAAGCTTG TGCCCAGACC GGGTAGTGGT
    AGCTCGTCAG GATCAAGTAA CTCTGGATCC CAAGCAGGAT CCCACCCAGG ATCTCAGAGT
    GGATCTGGAG AGCGCTTCCG GATGAGATCA TCTTCAAAAT CTGAGGGCTC ACCATCTCAA
    CGACATGAAA ACACAGCCAA AAAGTCTGAA GAGAAGAAAG AAGCAGTTAG ACAGGCCAAA
    GCTCCTGGAG AAGTGGATCT TACTGCACTC GCTAAGGAGC TTCGAGCAGT AGATGATGTT
    CGGCCACTTC ACAAAGTTAC TGACTATTCC TCATCCAGTG AAGAGTCTGG TACTACAGAT
    GAGGAGGATG ATGACATGGA ACAGGAAGGA GCAGATGAAT CGACTTCAGG CCCAGAGGAT
    GCTAGAGCTA TGTCATCTTT GAATTTAAGC AATGGAGAAA CGGAGTCTGT GAAAACCATG
    ATCGTACATG ATGATGTCGA AAGTGAACCT GCCATGACCC CATCTAAAGA AGGCACTTTA
    ATTGTCCGAC AGAGTACAGT TGACAAAAAG CGTGCCAACC AACTTGAGAG CAATGGCTTT
    GCGAGTCGTA TTCACCTCTT GCCAGATCTC TTACAGCAAA GCCATTCCTC CTCCACCACT
    TCTTCCCCAA CCTCAAGTCA ACCGACACCT ACCATGTCCC CCCAGACACC CCAGGACAAG
    CTAACTGCTA ATGAGACTCA GTCAGCTAGT AATACTCTCC AGAAACACAA ATCGTCCTCC
    TCTTTCACAC CGTTCATAGA TCCAAGGTTA CTTCAGATCT CTCCATCTAG TGGAACAACT
    GTTACGTCAG TAGGTACATA CATGCGCACA CTGGAAGCTA ACCTTAAAAA TACTAATTTT
    ATTTTAGTGG GATTTTCCAG TGAAGCGCTA AGGCCGGATG CCATGAGGCA AGATCCTACA
    AGGAAAGGAT CAGTTGTTAA TGTTAATCCC ACAAATACTC GGCCACAAAG TGATACACCA
    GAGATTCGCA AATACAAGAA GAGATTTAAT TCTGAGATTT TGTGTGCGGC ATTGTGGGGT
    GTAAATTTGC TGGTGGGAAC TGAAAGTGGT TTAATGCTGT TGGATAGAAG TGGACAAGGG
    AAAGTGTACC CCCTCATTAA CAGAAGGCGA TTTCAGCAGA TGGATGTGCT TGAAGGCTTG
    AATGTCTTGG TAACTATATC AGGGAAAAAG AACAAGCTGA GAGTATATTA CTTATCCTGG
    CTGAGGAATA AAATTTTGCA CAATGATCCT GAAGTAGAGA AGAAACAAGG ATGGACAACA
    GTTGGGGATT TGGAAGGCTG TGTGCACTAC AAAGTTGTAA AATATGAACG TATCAAGTTT
    TTGGTAATCG CTTTAAAAAG TTCAGTGGAG GTGTATGCCT GGGCACCGAA ACCATACCAC
    AAGTTTATGG CCTTTAAGTC ATTCGGAGAT TTAGTACACA GACCTCTCCT TGTAGACCTG
    ACTGTGGAAG AAGGACAGAG GCTGAAAGTG ATCTATGGTT CCTGTGCTGG GTTTCATGCA
    GTAGATGTGG ACTCGGGCGC AGTTTATGAT ATTTACTTGC CAACACATAT TCAGAGCAGC
    ATTCAGCCAC ACGCAATCAT CATTCTCCCA AATACTGATG GTATGGAACT GCTGGTTTGC
    TATGAAGATG AAGGAGTTTA TGTTAATACA TACGGCAGGA TCACCAAAGA TGTAGTTCTA
    CAATGGGGAG AAATGCCTAC ATCAGTTGCT TATATCCGAT CTAACCAGAT CATGGGATGG
    GGTGAAAAAG CTATTGAGAT TCGATCCGTG GAAACTGGCC ACCTTGATGG AGTATTTATG
    CACAAAAGGG CACAAAGGCT CAAGTTCTTG TGCGAACGAA ATGACAAGGT TTTCTTTGCC
    TCGGTGCGGT CTGGCGGAAG CAGCCAAGTG TATTTCATGA CTTTGGGCAG AAGCTCTCTT
    CTGAGCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa05831
    Clone ID Related Accession (Same CDS sequence) XM_004911783.3 , XM_004911783.4
    Accession Version XM_004911783.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4158bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004911783.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCCAACG ATTCGCCTGC CAAAAGTCTT GTGGATATAG ACCTGTCGTC TCTGAGGGAC 
    CCAGCTGGCA TCTTTGAATT AGTGGAAGTG GTAGGAAATG GCACCTATGG ACAAGTATAT
    AAGGGCCGAC ATGTCAAAAC AGGCCAACTT GCAGCCATCA AAGTCATGGA TGTTACAGAG
    GATGAAGAGG AAGAAATCAA ACTGGAAATC AATATGTTGA AGAAATATTC CCACCACAGA
    AATATAGCTA CTTATTATGG TGCTTTTATT AAGAAAAGTC CTCCTGGGCA TGATGACCAG
    CTTTGGTTGG TGATGGAGTT TTGTGGTGCT GGCTCAATCA CTGACCTGGT GAAGAATACA
    AAGGGGAATA GTTTGAAAGA AGACTGGATT GCCTATATCT CTCGTGAGAT ACTTAGGGGA
    TTGGCCCACC TCCATGCTCA TCATGTGATT CACCGGGATA TCAAAGGACA GAATGTGCTG
    CTAACAGAGA ATGCCGAAGT GAAATTAGTG GATTTTGGTG TTAGTGCACA GCTGGATAGG
    ACAGTGGGCC GAAGGAACAC ATTTATTGGT ACACCATACT GGATGGCTCC AGAAGTTATC
    GCCTGTGATG AGAACCCAGA TGCTACCTAT GACTATAGGA GTGACCTCTG GTCATGTGGT
    ATTACAGCGA TTGAAATGGC AGAAGGTGCT CCTCCTCTTT GCGATATGCA TCCAATGCGA
    GCCCTGTTCC TTATCCCAAG AAATCCTCCA CCACGGCTAA AATCCAAGAA ATGGTCAAAG
    AAGTTCTTCA GCTTTATAGA AGGCTGCCTT GTGAAAAATT ACATGCAACG TCCCCCAACA
    GAACAGCTTT TAAAGCATCC ATTTATACGA GATCAGCCAA ATGAAAGGCA AGTCCGAATT
    CAGCTGAAGG ATCACATAGA CAGGACAAGG AAGAAGAGAG GAGAAAAAGA TGAAACAGAG
    TATGAATACA GTGGAAGTGA AGAGGAGGAA GAGGAAGTGC CTGAACAGGA AGGAGAACCA
    AGTTCCATTG TTAATGTGCC TGGGGAATCA ACACTCCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGCTC TGAGGCTTTG CGACGGCAAC AGCTGCTGCA GGAGCAACAA
    CTCAGAGAGC AGGAGGAATA TAAAAGACAG CTCCTGGCAG AGAGACAGAA GCGCATAGAA
    CAGCAGAAAG AGCAAAGGAG GAGACTTGAA GAGCAACAAA GAAGAGAGCG TGAGGCTCGT
    CGGCAGCAGG AACGAGAACA AAGAAGGCGA GAGCAAGAGG AAAAGAAGAG AATGGAGGAG
    ATGGAAAGAA GGCGCAAGGA AGAGGAAGAA AGGAGAAGAG CAGAGGAAGA GAAAAGAAGA
    GTGGAAAGAG AGCAGGAGTA TATCCGAAGA CAGCTGGAGG AGGAGCAGCG GCACTTAGAA
    ATCCTTCAGC AGCAGCTGCT TCAGGAGCAG GCCATGTTAC TGGAGTATAG ATGGCGTGAA
    ATGGAGGAGC ACCGCCAGGC TGAAAGACTT CAGCGTCAGT TGCAGCAAGA GCAGGCCTAT
    CTCATGTCAC TACAGCATGA TCATAGACAG CAGCAGCCAC CACAACAGCA GCCCCAGCCC
    CAGCAGCCTC CACTGTCCCA GCAGGAAAGG AGCAAGCAAA CATACCATAC CCAAGAACCC
    AAGCCTCATT ATGAACAAGC AGAACGAATA CGAGAGGCAG AGGAGCGCCT TAGGAAAGCA
    AACCAAGGCT CCCCTGAAGC CCAGGCTAAG CAACCAGGAA GAGTTTTAGA GCCATTAGTG
    CCTTCAAGAT CCGAATCATT TTCCAATGGA AATTCTGAGC CTGCTCAACC TGCCATGCAC
    AGACCAGTGG AACCACAGGT ACAGTGGTCT CATCTGGCAT CCCTCAAGAA CAATGTATCC
    CCTGTCTCGC GATCCCATTC CTTCAGTGAC CCTTCTCCCA AATTTGCACC TCACCTTCTA
    CGATCTCAGG ATTCGCACCA AACTTCACGC AGTGAAGTGC AAAGTCAGGG TTCTGACCCT
    AAGTCTGAAA TCTCTGAGCC AACCCAAAGG GTTTGGGCTC GATCAGACAG TGACGAGGTG
    CCTCCAAGGG TGCCTGTGAG GACAACATCA AGATCTCCAG TATTGTCACG GAAAGATTCA
    CCACTGCAGG GCAGTGGGCA GCAAAATACC CAAGCTGGTC AGAGAAATTC CACAAGCAAT
    ATTGAGCCTA GACTTCTTTG GGAAAGAGTA GAGAAGCTTG TGCCCAGACC GGGTAGTGGT
    AGCTCGTCAG GATCAAGTAA CTCTGGATCC CAAGCAGGAT CCCACCCAGG ATCTCAGAGT
    GGATCTGGAG AGCGCTTCCG GATGAGATCA TCTTCAAAAT CTGAGGGCTC ACCATCTCAA
    CGACATGAAA ACACAGCCAA AAAGTCTGAA GAGAAGAAAG AAGCAGTTAG ACAGGCCAAA
    GCTCCTGGAG AAGTGGATCT TACTGCACTC GCTAAGGAGC TTCGAGCAGT AGATGATGTT
    CGGCCACTTC ACAAAGTTAC TGACTATTCC TCATCCAGTG AAGAGTCTGG TACTACAGAT
    GAGGAGGATG ATGACATGGA ACAGGAAGGA GCAGATGAAT CGACTTCAGG CCCAGAGGAT
    GCTAGAGCTA TGTCATCTTT GAATTTAAGC AATGGAGAAA CGGAGTCTGT GAAAACCATG
    ATCGTACATG ATGATGTCGA AAGTGAACCT GCCATGACCC CATCTAAAGA AGGCACTTTA
    ATTGTCCGAC AGAGTACAGT TGACAAAAAG CGTGCCAACC AACTTGAGAG CAATGGCTTT
    GCGAGTCGTA TTCACCTCTT GCCAGATCTC TTACAGCAAA GCCATTCCTC CTCCACCACT
    TCTTCCCCAA CCTCAAGTCA ACCGACACCT ACCATGTCCC CCCAGACACC CCAGGACAAG
    CTAACTGCTA ATGAGACTCA GTCAGCTAGT AATACTCTCC AGAAACACAA ATCGTCCTCC
    TCTTTCACAC CGTTCATAGA TCCAAGGTTA CTTCAGATCT CTCCATCTAG TGGAACAACT
    GTTACGTCAG TAGTGGGATT TTCCAGTGAA GCGCTAAGGC CGGATGCCAT GAGGCAAGAT
    CCTACAAGGA AAGGATCAGT TGTTAATGTT AATCCCACAA ATACTCGGCC ACAAAGTGAT
    ACACCAGAGA TTCGCAAATA CAAGAAGAGA TTTAATTCTG AGATTTTGTG TGCGGCATTG
    TGGGGTGTAA ATTTGCTGGT GGGAACTGAA AGTGGTTTAA TGCTGTTGGA TAGAAGTGGA
    CAAGGGAAAG TGTACCCCCT CATTAACAGA AGGCGATTTC AGCAGATGGA TGTGCTTGAA
    GGCTTGAATG TCTTGGTAAC TATATCAGGG AAAAAGAACA AGCTGAGAGT ATATTACTTA
    TCCTGGCTGA GGAATAAAAT TTTGCACAAT GATCCTGAAG TAGAGAAGAA ACAAGGATGG
    ACAACAGTTG GGGATTTGGA AGGCTGTGTG CACTACAAAG TTGTAAAATA TGAACGTATC
    AAGTTTTTGG TAATCGCTTT AAAAAGTTCA GTGGAGGTGT ATGCCTGGGC ACCGAAACCA
    TACCACAAGT TTATGGCCTT TAAGTCATTC GGAGATTTAG TACACAGACC TCTCCTTGTA
    GACCTGACTG TGGAAGAAGG ACAGAGGCTG AAAGTGATCT ATGGTTCCTG TGCTGGGTTT
    CATGCAGTAG ATGTGGACTC GGGCGCAGTT TATGATATTT ACTTGCCAAC ACATATTCAG
    AGCAGCATTC AGCCACACGC AATCATCATT CTCCCAAATA CTGATGGTAT GGAACTGCTG
    GTTTGCTATG AAGATGAAGG AGTTTATGTT AATACATACG GCAGGATCAC CAAAGATGTA
    GTTCTACAAT GGGGAGAAAT GCCTACATCA GTTGCTTATA TCCGATCTAA CCAGATCATG
    GGATGGGGTG AAAAAGCTAT TGAGATTCGA TCCGTGGAAA CTGGCCACCT TGATGGAGTA
    TTTATGCACA AAAGGGCACA AAGGCTCAAG TTCTTGTGCG AACGAAATGA CAAGGTTTTC
    TTTGCCTCGG TGCGGTCTGG CGGAAGCAGC CAAGTGTATT TCATGACTTT GGGCAGAAGC
    TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004911840.1
    CDS343..4500
    Translation

    Target ORF information:

    RefSeq Version XM_004911783.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 4 (map4k4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004911783.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCCAACG ATTCGCCTGC CAAAAGTCTT GTGGATATAG ACCTGTCGTC TCTGAGGGAC 
    CCAGCTGGCA TCTTTGAATT AGTGGAAGTG GTAGGAAATG GCACCTATGG ACAAGTATAT
    AAGGGCCGAC ATGTCAAAAC AGGCCAACTT GCAGCCATCA AAGTCATGGA TGTTACAGAG
    GATGAAGAGG AAGAAATCAA ACTGGAAATC AATATGTTGA AGAAATATTC CCACCACAGA
    AATATAGCTA CTTATTATGG TGCTTTTATT AAGAAAAGTC CTCCTGGGCA TGATGACCAG
    CTTTGGTTGG TGATGGAGTT TTGTGGTGCT GGCTCAATCA CTGACCTGGT GAAGAATACA
    AAGGGGAATA GTTTGAAAGA AGACTGGATT GCCTATATCT CTCGTGAGAT ACTTAGGGGA
    TTGGCCCACC TCCATGCTCA TCATGTGATT CACCGGGATA TCAAAGGACA GAATGTGCTG
    CTAACAGAGA ATGCCGAAGT GAAATTAGTG GATTTTGGTG TTAGTGCACA GCTGGATAGG
    ACAGTGGGCC GAAGGAACAC ATTTATTGGT ACACCATACT GGATGGCTCC AGAAGTTATC
    GCCTGTGATG AGAACCCAGA TGCTACCTAT GACTATAGGA GTGACCTCTG GTCATGTGGT
    ATTACAGCGA TTGAAATGGC AGAAGGTGCT CCTCCTCTTT GCGATATGCA TCCAATGCGA
    GCCCTGTTCC TTATCCCAAG AAATCCTCCA CCACGGCTAA AATCCAAGAA ATGGTCAAAG
    AAGTTCTTCA GCTTTATAGA AGGCTGCCTT GTGAAAAATT ACATGCAACG TCCCCCAACA
    GAACAGCTTT TAAAGCATCC ATTTATACGA GATCAGCCAA ATGAAAGGCA AGTCCGAATT
    CAGCTGAAGG ATCACATAGA CAGGACAAGG AAGAAGAGAG GAGAAAAAGA TGAAACAGAG
    TATGAATACA GTGGAAGTGA AGAGGAGGAA GAGGAAGTGC CTGAACAGGA AGGAGAACCA
    AGTTCCATTG TTAATGTGCC TGGGGAATCA ACACTCCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGCTC TGAGGCTTTG CGACGGCAAC AGCTGCTGCA GGAGCAACAA
    CTCAGAGAGC AGGAGGAATA TAAAAGACAG CTCCTGGCAG AGAGACAGAA GCGCATAGAA
    CAGCAGAAAG AGCAAAGGAG GAGACTTGAA GAGCAACAAA GAAGAGAGCG TGAGGCTCGT
    CGGCAGCAGG AACGAGAACA AAGAAGGCGA GAGCAAGAGG AAAAGAAGAG AATGGAGGAG
    ATGGAAAGAA GGCGCAAGGA AGAGGAAGAA AGGAGAAGAG CAGAGGAAGA GAAAAGAAGA
    GTGGAAAGAG AGCAGGAGTA TATCCGAAGA CAGCTGGAGG AGGAGCAGCG GCACTTAGAA
    ATCCTTCAGC AGCAGCTGCT TCAGGAGCAG GCCATGTTAC TGGAGTATAG ATGGCGTGAA
    ATGGAGGAGC ACCGCCAGGC TGAAAGACTT CAGCGTCAGT TGCAGCAAGA GCAGGCCTAT
    CTCATGTCAC TACAGCATGA TCATAGACAG CAGCAGCCAC CACAACAGCA GCCCCAGCCC
    CAGCAGCCTC CACTGTCCCA GCAGGAAAGG AGCAAGCAAA CATACCATAC CCAAGAACCC
    AAGCCTCATT ATGAACAAGC AGAACGAATA CGAGAGGCAG AGGAGCGCCT TAGGAAAGCA
    AACCAAGGCT CCCCTGAAGC CCAGGCTAAG CAACCAGGAA GAGTTTTAGA GCCATTAGTG
    CCTTCAAGAT CCGAATCATT TTCCAATGGA AATTCTGAGC CTGCTCAACC TGCCATGCAC
    AGACCAGTGG AACCACAGGT ACAGTGGTCT CATCTGGCAT CCCTCAAGAA CAATGTATCC
    CCTGTCTCGC GATCCCATTC CTTCAGTGAC CCTTCTCCCA AATTTGCACC TCACCTTCTA
    CGATCTCAGG ATTCGCACCA AACTTCACGC AGTGAAGTGC AAAGTCAGGG TTCTGACCCT
    AAGTCTGAAA TCTCTGAGCC AACCCAAAGG GTTTGGGCTC GATCAGACAG TGACGAGGTG
    CCTCCAAGGG TGCCTGTGAG GACAACATCA AGATCTCCAG TATTGTCACG GAAAGATTCA
    CCACTGCAGG GCAGTGGGCA GCAAAATACC CAAGCTGGTC AGAGAAATTC CACAAGCAAT
    ATTGAGCCTA GACTTCTTTG GGAAAGAGTA GAGAAGCTTG TGCCCAGACC GGGTAGTGGT
    AGCTCGTCAG GATCAAGTAA CTCTGGATCC CAAGCAGGAT CCCACCCAGG ATCTCAGAGT
    GGATCTGGAG AGCGCTTCCG GATGAGATCA TCTTCAAAAT CTGAGGGCTC ACCATCTCAA
    CGACATGAAA ACACAGCCAA AAAGTCTGAA GAGAAGAAAG AAGCAGTTAG ACAGGCCAAA
    GCTCCTGGAG AAGTGGATCT TACTGCACTC GCTAAGGAGC TTCGAGCAGT AGATGATGTT
    CGGCCACTTC ACAAAGTTAC TGACTATTCC TCATCCAGTG AAGAGTCTGG TACTACAGAT
    GAGGAGGATG ATGACATGGA ACAGGAAGGA GCAGATGAAT CGACTTCAGG CCCAGAGGAT
    GCTAGAGCTA TGTCATCTTT GAATTTAAGC AATGGAGAAA CGGAGTCTGT GAAAACCATG
    ATCGTACATG ATGATGTCGA AAGTGAACCT GCCATGACCC CATCTAAAGA AGGCACTTTA
    ATTGTCCGAC AGAGTACAGT TGACAAAAAG CGTGCCAACC AACTTGAGAG CAATGGCTTT
    GCGAGTCGTA TTCACCTCTT GCCAGATCTC TTACAGCAAA GCCATTCCTC CTCCACCACT
    TCTTCCCCAA CCTCAAGTCA ACCGACACCT ACCATGTCCC CCCAGACACC CCAGGACAAG
    CTAACTGCTA ATGAGACTCA GTCAGCTAGT AATACTCTCC AGAAACACAA ATCGTCCTCC
    TCTTTCACAC CGTTCATAGA TCCAAGGTTA CTTCAGATCT CTCCATCTAG TGGAACAACT
    GTTACGTCAG TAGTGGGATT TTCCAGTGAA GCGCTAAGGC CGGATGCCAT GAGGCAAGAT
    CCTACAAGGA AAGGATCAGT TGTTAATGTT AATCCCACAA ATACTCGGCC ACAAAGTGAT
    ACACCAGAGA TTCGCAAATA CAAGAAGAGA TTTAATTCTG AGATTTTGTG TGCGGCATTG
    TGGGGTGTAA ATTTGCTGGT GGGAACTGAA AGTGGTTTAA TGCTGTTGGA TAGAAGTGGA
    CAAGGGAAAG TGTACCCCCT CATTAACAGA AGGCGATTTC AGCAGATGGA TGTGCTTGAA
    GGCTTGAATG TCTTGGTAAC TATATCAGGG AAAAAGAACA AGCTGAGAGT ATATTACTTA
    TCCTGGCTGA GGAATAAAAT TTTGCACAAT GATCCTGAAG TAGAGAAGAA ACAAGGATGG
    ACAACAGTTG GGGATTTGGA AGGCTGTGTG CACTACAAAG TTGTAAAATA TGAACGTATC
    AAGTTTTTGG TAATCGCTTT AAAAAGTTCA GTGGAGGTGT ATGCCTGGGC ACCGAAACCA
    TACCACAAGT TTATGGCCTT TAAGTCATTC GGAGATTTAG TACACAGACC TCTCCTTGTA
    GACCTGACTG TGGAAGAAGG ACAGAGGCTG AAAGTGATCT ATGGTTCCTG TGCTGGGTTT
    CATGCAGTAG ATGTGGACTC GGGCGCAGTT TATGATATTT ACTTGCCAAC ACATATTCAG
    AGCAGCATTC AGCCACACGC AATCATCATT CTCCCAAATA CTGATGGTAT GGAACTGCTG
    GTTTGCTATG AAGATGAAGG AGTTTATGTT AATACATACG GCAGGATCAC CAAAGATGTA
    GTTCTACAAT GGGGAGAAAT GCCTACATCA GTTGCTTATA TCCGATCTAA CCAGATCATG
    GGATGGGGTG AAAAAGCTAT TGAGATTCGA TCCGTGGAAA CTGGCCACCT TGATGGAGTA
    TTTATGCACA AAAGGGCACA AAGGCTCAAG TTCTTGTGCG AACGAAATGA CAAGGTTTTC
    TTTGCCTCGG TGCGGTCTGG CGGAAGCAGC CAAGTGTATT TCATGACTTT GGGCAGAAGC
    TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa05832
    Clone ID Related Accession (Same CDS sequence) XM_004911784.3 , XM_004911784.4
    Accession Version XM_004911784.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4125bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004911784.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCCAACG ATTCGCCTGC CAAAAGTCTT GTGGATATAG ACCTGTCGTC TCTGAGGGAC 
    CCAGCTGGCA TCTTTGAATT AGTGGAAGTG GTAGGAAATG GCACCTATGG ACAAGTATAT
    AAGGGCCGAC ATGTCAAAAC AGGCCAACTT GCAGCCATCA AAGTCATGGA TGTTACAGAG
    GATGAAGAGG AAGAAATCAA ACTGGAAATC AATATGTTGA AGAAATATTC CCACCACAGA
    AATATAGCTA CTTATTATGG TGCTTTTATT AAGAAAAGTC CTCCTGGGCA TGATGACCAG
    CTTTGGTTGG TGATGGAGTT TTGTGGTGCT GGCTCAATCA CTGACCTGGT GAAGAATACA
    AAGGGGAATA GTTTGAAAGA AGACTGGATT GCCTATATCT CTCGTGAGAT ACTTAGGGGA
    TTGGCCCACC TCCATGCTCA TCATGTGATT CACCGGGATA TCAAAGGACA GAATGTGCTG
    CTAACAGAGA ATGCCGAAGT GAAATTAGTG GATTTTGGTG TTAGTGCACA GCTGGATAGG
    ACAGTGGGCC GAAGGAACAC ATTTATTGGT ACACCATACT GGATGGCTCC AGAAGTTATC
    GCCTGTGATG AGAACCCAGA TGCTACCTAT GACTATAGGA GTGACCTCTG GTCATGTGGT
    ATTACAGCGA TTGAAATGGC AGAAGGTGCT CCTCCTCTTT GCGATATGCA TCCAATGCGA
    GCCCTGTTCC TTATCCCAAG AAATCCTCCA CCACGGCTAA AATCCAAGAA ATGGTCAAAG
    AAGTTCTTCA GCTTTATAGA AGGCTGCCTT GTGAAAAATT ACATGCAACG TCCCCCAACA
    GAACAGCTTT TAAAGCATCC ATTTATACGA GATCAGCCAA ATGAAAGGCA AGTCCGAATT
    CAGCTGAAGG ATCACATAGA CAGGACAAGG AAGAAGAGAG GAGAAAAAGA TGAAACAGAG
    TATGAATACA GTGGAAGTGA AGAGGAGGAA GAGGAAGTGC CTGAACAGGA AGGAGAACCA
    AGTTCCATTG TTAATGTGCC TGGGGAATCA ACACTCCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGCTC TGAGGCTTTG CGACGGCAAC AGCTGCTGCA GGAGCAACAA
    CTCAGAGAGC AGGAGGAATA TAAAAGACAG CTCCTGGCAG AGAGACAGAA GCGCATAGAA
    CAGCAGAAAG AGCAAAGGAG GAGACTTGAA GAGCAACAAA GAAGAGAGCG TGAGGCTCGT
    CGGCAGCAGG AACGAGAACA AAGAAGGCGA GAGCAAGAGG AAAAGAAGAG AATGGAGGAG
    ATGGAAAGAA GGCGCAAGGA AGAGGAAGAA AGGAGAAGAG CAGAGGAAGA GAAAAGAAGA
    GTGGAAAGAG AGCAGGAGTA TAGATGGCGT GAAATGGAGG AGCACCGCCA GGCTGAAAGA
    CTTCAGCGTC AGTTGCAGCA AGAGCAGGCC TATCTCATGT CACTACAGCA TGATCATAGA
    CAGCAGCAGC CACCACAACA GCAGCCCCAG CCCCAGCAGC CTCCACTGTC CCAGCAGGAA
    AGGAGCAAGC AAACATACCA TACCCAAGAA CCCAAGCCTC ATTATGAACA AGCAGAACGA
    ATACGAGAGG CAGAGGAGCG CCTTAGGAAA GCAAACCAAG GCTCCCCTGA AGCCCAGGCT
    AAGCAACCAG GAAGAGTTTT AGAGCCATTA GTGCCTTCAA GATCCGAATC ATTTTCCAAT
    GGAAATTCTG AGCCTGCTCA ACCTGCCATG CACAGACCAG TGGAACCACA GGTACAGTGG
    TCTCATCTGG CATCCCTCAA GAACAATGTA TCCCCTGTCT CGCGATCCCA TTCCTTCAGT
    GACCCTTCTC CCAAATTTGC ACCTCACCTT CTACGATCTC AGGATTCGCA CCAAACTTCA
    CGCAGTGAAG TGCAAAGTCA GGGTTCTGAC CCTAAGTCTG AAATCTCTGA GCCAACCCAA
    AGGGTTTGGG CTCGATCAGA CAGTGACGAG GTGCCTCCAA GGGTGCCTGT GAGGACAACA
    TCAAGATCTC CAGTATTGTC ACGGAAAGAT TCACCACTGC AGGGCAGTGG GCAGCAAAAT
    ACCCAAGCTG GTCAGAGAAA TTCCACAAGC AATATTGAGC CTAGACTTCT TTGGGAAAGA
    GTAGAGAAGC TTGTGCCCAG ACCGGGTAGT GGTAGCTCGT CAGGATCAAG TAACTCTGGA
    TCCCAAGCAG GATCCCACCC AGGATCTCAG AGTGGATCTG GAGAGCGCTT CCGGATGAGA
    TCATCTTCAA AATCTGAGGG CTCACCATCT CAACGACATG AAAACACAGC CAAAAAGTCT
    GAAGAGAAGA AAGAAGCAGT TAGACAGGCC AAAGCTCCTG GAGAAGTGGA TCTTACTGCA
    CTCGCTAAGG AGCTTCGAGC AGTAGATGAT GTTCGGCCAC TTCACAAAGT TACTGACTAT
    TCCTCATCCA GTGAAGAGTC TGGTACTACA GATGAGGAGG ATGATGACAT GGAACAGGAA
    GGAGCAGATG AATCGACTTC AGGCCCAGAG GATGCTAGAG CTATGTCATC TTTGAATTTA
    AGCAATGGAG AAACGGAGTC TGTGAAAACC ATGATCGTAC ATGATGATGT CGAAAGTGAA
    CCTGCCATGA CCCCATCTAA AGAAGGCACT TTAATTGTCC GACAGAGTAC AGTTGACAAA
    AAGCGTGCCA ACCAACTTGA GAGCAATGGC TTTGCGAGTC GTATTCACCT CTTGCCAGAT
    CTCTTACAGC AAAGCCATTC CTCCTCCACC ACTTCTTCCC CAACCTCAAG TCAACCGACA
    CCTACCATGT CCCCCCAGAC ACCCCAGGAC AAGCTAACTG CTAATGAGAC TCAGTCAGCT
    AGTAATACTC TCCAGAAACA CAAATCGTCC TCCTCTTTCA CACCGTTCAT AGATCCAAGG
    TTACTTCAGA TCTCTCCATC TAGTGGAACA ACTGTTACGT CAGTAGGTAC ATACATGCGC
    ACACTGGAAG CTAACCTTAA AAATACTAAT TTTATTTTAG TGGGATTTTC CAGTGAAGCG
    CTAAGGCCGG ATGCCATGAG GCAAGATCCT ACAAGGAAAG GATCAGTTGT TAATGTTAAT
    CCCACAAATA CTCGGCCACA AAGTGATACA CCAGAGATTC GCAAATACAA GAAGAGATTT
    AATTCTGAGA TTTTGTGTGC GGCATTGTGG GGTGTAAATT TGCTGGTGGG AACTGAAAGT
    GGTTTAATGC TGTTGGATAG AAGTGGACAA GGGAAAGTGT ACCCCCTCAT TAACAGAAGG
    CGATTTCAGC AGATGGATGT GCTTGAAGGC TTGAATGTCT TGGTAACTAT ATCAGGGAAA
    AAGAACAAGC TGAGAGTATA TTACTTATCC TGGCTGAGGA ATAAAATTTT GCACAATGAT
    CCTGAAGTAG AGAAGAAACA AGGATGGACA ACAGTTGGGG ATTTGGAAGG CTGTGTGCAC
    TACAAAGTTG TAAAATATGA ACGTATCAAG TTTTTGGTAA TCGCTTTAAA AAGTTCAGTG
    GAGGTGTATG CCTGGGCACC GAAACCATAC CACAAGTTTA TGGCCTTTAA GTCATTCGGA
    GATTTAGTAC ACAGACCTCT CCTTGTAGAC CTGACTGTGG AAGAAGGACA GAGGCTGAAA
    GTGATCTATG GTTCCTGTGC TGGGTTTCAT GCAGTAGATG TGGACTCGGG CGCAGTTTAT
    GATATTTACT TGCCAACACA TATTCAGAGC AGCATTCAGC CACACGCAAT CATCATTCTC
    CCAAATACTG ATGGTATGGA ACTGCTGGTT TGCTATGAAG ATGAAGGAGT TTATGTTAAT
    ACATACGGCA GGATCACCAA AGATGTAGTT CTACAATGGG GAGAAATGCC TACATCAGTT
    GCTTATATCC GATCTAACCA GATCATGGGA TGGGGTGAAA AAGCTATTGA GATTCGATCC
    GTGGAAACTG GCCACCTTGA TGGAGTATTT ATGCACAAAA GGGCACAAAG GCTCAAGTTC
    TTGTGCGAAC GAAATGACAA GGTTTTCTTT GCCTCGGTGC GGTCTGGCGG AAGCAGCCAA
    GTGTATTTCA TGACTTTGGG CAGAAGCTCT CTTCTGAGCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004911841.1
    CDS344..4468
    Translation

    Target ORF information:

    RefSeq Version XM_004911784.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 4 (map4k4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004911784.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCCAACG ATTCGCCTGC CAAAAGTCTT GTGGATATAG ACCTGTCGTC TCTGAGGGAC 
    CCAGCTGGCA TCTTTGAATT AGTGGAAGTG GTAGGAAATG GCACCTATGG ACAAGTATAT
    AAGGGCCGAC ATGTCAAAAC AGGCCAACTT GCAGCCATCA AAGTCATGGA TGTTACAGAG
    GATGAAGAGG AAGAAATCAA ACTGGAAATC AATATGTTGA AGAAATATTC CCACCACAGA
    AATATAGCTA CTTATTATGG TGCTTTTATT AAGAAAAGTC CTCCTGGGCA TGATGACCAG
    CTTTGGTTGG TGATGGAGTT TTGTGGTGCT GGCTCAATCA CTGACCTGGT GAAGAATACA
    AAGGGGAATA GTTTGAAAGA AGACTGGATT GCCTATATCT CTCGTGAGAT ACTTAGGGGA
    TTGGCCCACC TCCATGCTCA TCATGTGATT CACCGGGATA TCAAAGGACA GAATGTGCTG
    CTAACAGAGA ATGCCGAAGT GAAATTAGTG GATTTTGGTG TTAGTGCACA GCTGGATAGG
    ACAGTGGGCC GAAGGAACAC ATTTATTGGT ACACCATACT GGATGGCTCC AGAAGTTATC
    GCCTGTGATG AGAACCCAGA TGCTACCTAT GACTATAGGA GTGACCTCTG GTCATGTGGT
    ATTACAGCGA TTGAAATGGC AGAAGGTGCT CCTCCTCTTT GCGATATGCA TCCAATGCGA
    GCCCTGTTCC TTATCCCAAG AAATCCTCCA CCACGGCTAA AATCCAAGAA ATGGTCAAAG
    AAGTTCTTCA GCTTTATAGA AGGCTGCCTT GTGAAAAATT ACATGCAACG TCCCCCAACA
    GAACAGCTTT TAAAGCATCC ATTTATACGA GATCAGCCAA ATGAAAGGCA AGTCCGAATT
    CAGCTGAAGG ATCACATAGA CAGGACAAGG AAGAAGAGAG GAGAAAAAGA TGAAACAGAG
    TATGAATACA GTGGAAGTGA AGAGGAGGAA GAGGAAGTGC CTGAACAGGA AGGAGAACCA
    AGTTCCATTG TTAATGTGCC TGGGGAATCA ACACTCCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGCTC TGAGGCTTTG CGACGGCAAC AGCTGCTGCA GGAGCAACAA
    CTCAGAGAGC AGGAGGAATA TAAAAGACAG CTCCTGGCAG AGAGACAGAA GCGCATAGAA
    CAGCAGAAAG AGCAAAGGAG GAGACTTGAA GAGCAACAAA GAAGAGAGCG TGAGGCTCGT
    CGGCAGCAGG AACGAGAACA AAGAAGGCGA GAGCAAGAGG AAAAGAAGAG AATGGAGGAG
    ATGGAAAGAA GGCGCAAGGA AGAGGAAGAA AGGAGAAGAG CAGAGGAAGA GAAAAGAAGA
    GTGGAAAGAG AGCAGGAGTA TAGATGGCGT GAAATGGAGG AGCACCGCCA GGCTGAAAGA
    CTTCAGCGTC AGTTGCAGCA AGAGCAGGCC TATCTCATGT CACTACAGCA TGATCATAGA
    CAGCAGCAGC CACCACAACA GCAGCCCCAG CCCCAGCAGC CTCCACTGTC CCAGCAGGAA
    AGGAGCAAGC AAACATACCA TACCCAAGAA CCCAAGCCTC ATTATGAACA AGCAGAACGA
    ATACGAGAGG CAGAGGAGCG CCTTAGGAAA GCAAACCAAG GCTCCCCTGA AGCCCAGGCT
    AAGCAACCAG GAAGAGTTTT AGAGCCATTA GTGCCTTCAA GATCCGAATC ATTTTCCAAT
    GGAAATTCTG AGCCTGCTCA ACCTGCCATG CACAGACCAG TGGAACCACA GGTACAGTGG
    TCTCATCTGG CATCCCTCAA GAACAATGTA TCCCCTGTCT CGCGATCCCA TTCCTTCAGT
    GACCCTTCTC CCAAATTTGC ACCTCACCTT CTACGATCTC AGGATTCGCA CCAAACTTCA
    CGCAGTGAAG TGCAAAGTCA GGGTTCTGAC CCTAAGTCTG AAATCTCTGA GCCAACCCAA
    AGGGTTTGGG CTCGATCAGA CAGTGACGAG GTGCCTCCAA GGGTGCCTGT GAGGACAACA
    TCAAGATCTC CAGTATTGTC ACGGAAAGAT TCACCACTGC AGGGCAGTGG GCAGCAAAAT
    ACCCAAGCTG GTCAGAGAAA TTCCACAAGC AATATTGAGC CTAGACTTCT TTGGGAAAGA
    GTAGAGAAGC TTGTGCCCAG ACCGGGTAGT GGTAGCTCGT CAGGATCAAG TAACTCTGGA
    TCCCAAGCAG GATCCCACCC AGGATCTCAG AGTGGATCTG GAGAGCGCTT CCGGATGAGA
    TCATCTTCAA AATCTGAGGG CTCACCATCT CAACGACATG AAAACACAGC CAAAAAGTCT
    GAAGAGAAGA AAGAAGCAGT TAGACAGGCC AAAGCTCCTG GAGAAGTGGA TCTTACTGCA
    CTCGCTAAGG AGCTTCGAGC AGTAGATGAT GTTCGGCCAC TTCACAAAGT TACTGACTAT
    TCCTCATCCA GTGAAGAGTC TGGTACTACA GATGAGGAGG ATGATGACAT GGAACAGGAA
    GGAGCAGATG AATCGACTTC AGGCCCAGAG GATGCTAGAG CTATGTCATC TTTGAATTTA
    AGCAATGGAG AAACGGAGTC TGTGAAAACC ATGATCGTAC ATGATGATGT CGAAAGTGAA
    CCTGCCATGA CCCCATCTAA AGAAGGCACT TTAATTGTCC GACAGAGTAC AGTTGACAAA
    AAGCGTGCCA ACCAACTTGA GAGCAATGGC TTTGCGAGTC GTATTCACCT CTTGCCAGAT
    CTCTTACAGC AAAGCCATTC CTCCTCCACC ACTTCTTCCC CAACCTCAAG TCAACCGACA
    CCTACCATGT CCCCCCAGAC ACCCCAGGAC AAGCTAACTG CTAATGAGAC TCAGTCAGCT
    AGTAATACTC TCCAGAAACA CAAATCGTCC TCCTCTTTCA CACCGTTCAT AGATCCAAGG
    TTACTTCAGA TCTCTCCATC TAGTGGAACA ACTGTTACGT CAGTAGGTAC ATACATGCGC
    ACACTGGAAG CTAACCTTAA AAATACTAAT TTTATTTTAG TGGGATTTTC CAGTGAAGCG
    CTAAGGCCGG ATGCCATGAG GCAAGATCCT ACAAGGAAAG GATCAGTTGT TAATGTTAAT
    CCCACAAATA CTCGGCCACA AAGTGATACA CCAGAGATTC GCAAATACAA GAAGAGATTT
    AATTCTGAGA TTTTGTGTGC GGCATTGTGG GGTGTAAATT TGCTGGTGGG AACTGAAAGT
    GGTTTAATGC TGTTGGATAG AAGTGGACAA GGGAAAGTGT ACCCCCTCAT TAACAGAAGG
    CGATTTCAGC AGATGGATGT GCTTGAAGGC TTGAATGTCT TGGTAACTAT ATCAGGGAAA
    AAGAACAAGC TGAGAGTATA TTACTTATCC TGGCTGAGGA ATAAAATTTT GCACAATGAT
    CCTGAAGTAG AGAAGAAACA AGGATGGACA ACAGTTGGGG ATTTGGAAGG CTGTGTGCAC
    TACAAAGTTG TAAAATATGA ACGTATCAAG TTTTTGGTAA TCGCTTTAAA AAGTTCAGTG
    GAGGTGTATG CCTGGGCACC GAAACCATAC CACAAGTTTA TGGCCTTTAA GTCATTCGGA
    GATTTAGTAC ACAGACCTCT CCTTGTAGAC CTGACTGTGG AAGAAGGACA GAGGCTGAAA
    GTGATCTATG GTTCCTGTGC TGGGTTTCAT GCAGTAGATG TGGACTCGGG CGCAGTTTAT
    GATATTTACT TGCCAACACA TATTCAGAGC AGCATTCAGC CACACGCAAT CATCATTCTC
    CCAAATACTG ATGGTATGGA ACTGCTGGTT TGCTATGAAG ATGAAGGAGT TTATGTTAAT
    ACATACGGCA GGATCACCAA AGATGTAGTT CTACAATGGG GAGAAATGCC TACATCAGTT
    GCTTATATCC GATCTAACCA GATCATGGGA TGGGGTGAAA AAGCTATTGA GATTCGATCC
    GTGGAAACTG GCCACCTTGA TGGAGTATTT ATGCACAAAA GGGCACAAAG GCTCAAGTTC
    TTGTGCGAAC GAAATGACAA GGTTTTCTTT GCCTCGGTGC GGTCTGGCGG AAGCAGCCAA
    GTGTATTTCA TGACTTTGGG CAGAAGCTCT CTTCTGAGCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa05833
    Clone ID Related Accession (Same CDS sequence) XM_004911785.3 , XM_004911785.4
    Accession Version XM_004911785.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4029bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004911785.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCCAACG ATTCGCCTGC CAAAAGTCTT GTGGATATAG ACCTGTCGTC TCTGAGGGAC 
    CCAGCTGGCA TCTTTGAATT AGTGGAAGTG GTAGGAAATG GCACCTATGG ACAAGTATAT
    AAGGGCCGAC ATGTCAAAAC AGGCCAACTT GCAGCCATCA AAGTCATGGA TGTTACAGAG
    GATGAAGAGG AAGAAATCAA ACTGGAAATC AATATGTTGA AGAAATATTC CCACCACAGA
    AATATAGCTA CTTATTATGG TGCTTTTATT AAGAAAAGTC CTCCTGGGCA TGATGACCAG
    CTTTGGTTGG TGATGGAGTT TTGTGGTGCT GGCTCAATCA CTGACCTGGT GAAGAATACA
    AAGGGGAATA GTTTGAAAGA AGACTGGATT GCCTATATCT CTCGTGAGAT ACTTAGGGGA
    TTGGCCCACC TCCATGCTCA TCATGTGATT CACCGGGATA TCAAAGGACA GAATGTGCTG
    CTAACAGAGA ATGCCGAAGT GAAATTAGTG GATTTTGGTG TTAGTGCACA GCTGGATAGG
    ACAGTGGGCC GAAGGAACAC ATTTATTGGT ACACCATACT GGATGGCTCC AGAAGTTATC
    GCCTGTGATG AGAACCCAGA TGCTACCTAT GACTATAGGA GTGACCTCTG GTCATGTGGT
    ATTACAGCGA TTGAAATGGC AGAAGGTGCT CCTCCTCTTT GCGATATGCA TCCAATGCGA
    GCCCTGTTCC TTATCCCAAG AAATCCTCCA CCACGGCTAA AATCCAAGAA ATGGTCAAAG
    AAGTTCTTCA GCTTTATAGA AGGCTGCCTT GTGAAAAATT ACATGCAACG TCCCCCAACA
    GAACAGCTTT TAAAGCATCC ATTTATACGA GATCAGCCAA ATGAAAGGCA AGTCCGAATT
    CAGCTGAAGG ATCACATAGA CAGGACAAGG AAGAAGAGAG GAGAAAAAGA TGAAACAGAG
    TATGAATACA GTGGAAGTGA AGAGGAGGAA GAGGAAGTGC CTGAACAGGA AGGAGAACCA
    AGTTCCATTG TTAATGTGCC TGGGGAATCA ACACTCCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGCTC TGAGGCTTTG CGACGGCAAC AGCTGCTGCA GGAGCAACAA
    CTCAGAGAGC AGGAGGAATA TAAAAGACAG CTCCTGGCAG AGAGACAGAA GCGCATAGAA
    CAGCAGAAAG AGCAAAGGAG GAGACTTGAA GAGCAACAAA GAAGAGAGCG TGAGGCTCGT
    CGGCAGCAGG AACGAGAACA AAGAAGGCGA GAGCAAGAGG AAAAGAAGAG AATGGAGGAG
    ATGGAAAGAA GGCGCAAGGA AGAGGAAGAA AGGAGAAGAG CAGAGGAAGA GAAAAGAAGA
    GTGGAAAGAG AGCAGGAGTA TATCCGAAGA CAGCTGGAGG AGGAGCAGCG GCACTTAGAA
    ATCCTTCAGC AGCAGCTGCT TCAGGAGCAG GCCATGTTAC TGGAGTATAG ATGGCGTGAA
    ATGGAGGAGC ACCGCCAGGC TGAAAGACTT CAGCGTCAGT TGCAGCAAGA GCAGGCCTAT
    CTCATGTCAC TACAGCATGA TCATAGACAG CAGCAGCCAC CACAACAGCA GCCCCAGCCC
    CAGCAGCCTC CACTGTCCCA GCAGGAAAGG AGCAAGCAAA CATACCATAC CCAAGAACCC
    AAGCCTCATT ATGAACAAGC AGAACGAATA CGAGAGGCAG AGGAGCGCCT TAGGAAAGCA
    AACCAAGGCT CCCCTGAAGC CCAGGCTAAG CAACCAGGAA GAGTTTTAGA GCCATTAGTG
    CCTTCAAGAT CCGAATCATT TTCCAATGGA AATTCTGAGC CTGCTCAACC TGCCATGCAC
    AGACCAGTGG AACCACAGGT ACAGTGGTCT CATCTGGCAT CCCTCAAGAA CAATGTATCC
    CCTGTCTCGC GATCCCATTC CTTCAGTGAC CCTTCTCCCA AATTTGCACC TCACCTTCTA
    CGATCTCAGG ATTCGCACCA AACTTCACGC AGTGAAGTGC AAAGTCAGGG TTCTGACCCT
    AAGTCTGAAA TCTCTGAGCC AACCCAAAGG GTTTGGGCTC GATCAGACAG TGACGAGGTG
    CCTCCAAGGG TGCCTGTGAG GACAACATCA AGATCTCCAG TATTGTCACG GAAAGATTCA
    CCACTGCAGG GCAGTGGGCA GCAAAATACC CAAGCTGGTC AGAGAAATTC CACAAGCAAT
    ATTGAGCCTA GACTTCTTTG GGAAAGAGTA GAGAAGCTTG TGCCCAGACC GGGTAGTGGT
    AGCTCGTCAG GATCAAGTAA CTCTGGATCC CAAGCAGGAT CCCACCCAGG ATCTCAGAGT
    GGATCTGGAG AGCGCTTCCG GATGAGATCA TCTTCAAAAT CTGAGGGCTC ACCATCTCAA
    CGACATGAAA ACACAGCCAA AAAGTCTGAA GAGAAGAAAG AAGCAGTTAG ACAGGCCAAA
    GCTCCTGGAG AAGTGGATCT TACTGCACTC GCTAAGGAGC TTCGAGCAGT AGATGATGTT
    CGGCCACTTC ACAAAGTTAC TGACTATTCC TCATCCAGTG AAGAGTCTGG TACTACAGAT
    GAGGAGGATG ATGACATGGA ACAGGAAGGA GCAGATGAAT CGACTTCAGG CCCAGAGGAT
    GCTAGAGCTA TGTCATCTTT GAATTTAAGC AATGGAGAAA CGGAGTCTGT GAAAACCATG
    ATCGTACATG ATGATGTCGA AAGTGAACCT GCCATGACCC CATCTAAAGA AGGCACTTTA
    ATTGTCCGAC AGACTCAGTC AGCTAGTAAT ACTCTCCAGA AACACAAATC GTCCTCCTCT
    TTCACACCGT TCATAGATCC AAGGTTACTT CAGATCTCTC CATCTAGTGG AACAACTGTT
    ACGTCAGTAG GTACATACAT GCGCACACTG GAAGCTAACC TTAAAAATAC TAATTTTATT
    TTAGTGGGAT TTTCCAGTGA AGCGCTAAGG CCGGATGCCA TGAGGCAAGA TCCTACAAGG
    AAAGGATCAG TTGTTAATGT TAATCCCACA AATACTCGGC CACAAAGTGA TACACCAGAG
    ATTCGCAAAT ACAAGAAGAG ATTTAATTCT GAGATTTTGT GTGCGGCATT GTGGGGTGTA
    AATTTGCTGG TGGGAACTGA AAGTGGTTTA ATGCTGTTGG ATAGAAGTGG ACAAGGGAAA
    GTGTACCCCC TCATTAACAG AAGGCGATTT CAGCAGATGG ATGTGCTTGA AGGCTTGAAT
    GTCTTGGTAA CTATATCAGG GAAAAAGAAC AAGCTGAGAG TATATTACTT ATCCTGGCTG
    AGGAATAAAA TTTTGCACAA TGATCCTGAA GTAGAGAAGA AACAAGGATG GACAACAGTT
    GGGGATTTGG AAGGCTGTGT GCACTACAAA GTTGTAAAAT ATGAACGTAT CAAGTTTTTG
    GTAATCGCTT TAAAAAGTTC AGTGGAGGTG TATGCCTGGG CACCGAAACC ATACCACAAG
    TTTATGGCCT TTAAGTCATT CGGAGATTTA GTACACAGAC CTCTCCTTGT AGACCTGACT
    GTGGAAGAAG GACAGAGGCT GAAAGTGATC TATGGTTCCT GTGCTGGGTT TCATGCAGTA
    GATGTGGACT CGGGCGCAGT TTATGATATT TACTTGCCAA CACATATTCA GAGCAGCATT
    CAGCCACACG CAATCATCAT TCTCCCAAAT ACTGATGGTA TGGAACTGCT GGTTTGCTAT
    GAAGATGAAG GAGTTTATGT TAATACATAC GGCAGGATCA CCAAAGATGT AGTTCTACAA
    TGGGGAGAAA TGCCTACATC AGTTGCTTAT ATCCGATCTA ACCAGATCAT GGGATGGGGT
    GAAAAAGCTA TTGAGATTCG ATCCGTGGAA ACTGGCCACC TTGATGGAGT ATTTATGCAC
    AAAAGGGCAC AAAGGCTCAA GTTCTTGTGC GAACGAAATG ACAAGGTTTT CTTTGCCTCG
    GTGCGGTCTG GCGGAAGCAG CCAAGTGTAT TTCATGACTT TGGGCAGAAG CTCTCTTCTG
    AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004911842.1
    CDS344..4372
    Translation

    Target ORF information:

    RefSeq Version XM_004911785.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 4 (map4k4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004911785.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCCAACG ATTCGCCTGC CAAAAGTCTT GTGGATATAG ACCTGTCGTC TCTGAGGGAC 
    CCAGCTGGCA TCTTTGAATT AGTGGAAGTG GTAGGAAATG GCACCTATGG ACAAGTATAT
    AAGGGCCGAC ATGTCAAAAC AGGCCAACTT GCAGCCATCA AAGTCATGGA TGTTACAGAG
    GATGAAGAGG AAGAAATCAA ACTGGAAATC AATATGTTGA AGAAATATTC CCACCACAGA
    AATATAGCTA CTTATTATGG TGCTTTTATT AAGAAAAGTC CTCCTGGGCA TGATGACCAG
    CTTTGGTTGG TGATGGAGTT TTGTGGTGCT GGCTCAATCA CTGACCTGGT GAAGAATACA
    AAGGGGAATA GTTTGAAAGA AGACTGGATT GCCTATATCT CTCGTGAGAT ACTTAGGGGA
    TTGGCCCACC TCCATGCTCA TCATGTGATT CACCGGGATA TCAAAGGACA GAATGTGCTG
    CTAACAGAGA ATGCCGAAGT GAAATTAGTG GATTTTGGTG TTAGTGCACA GCTGGATAGG
    ACAGTGGGCC GAAGGAACAC ATTTATTGGT ACACCATACT GGATGGCTCC AGAAGTTATC
    GCCTGTGATG AGAACCCAGA TGCTACCTAT GACTATAGGA GTGACCTCTG GTCATGTGGT
    ATTACAGCGA TTGAAATGGC AGAAGGTGCT CCTCCTCTTT GCGATATGCA TCCAATGCGA
    GCCCTGTTCC TTATCCCAAG AAATCCTCCA CCACGGCTAA AATCCAAGAA ATGGTCAAAG
    AAGTTCTTCA GCTTTATAGA AGGCTGCCTT GTGAAAAATT ACATGCAACG TCCCCCAACA
    GAACAGCTTT TAAAGCATCC ATTTATACGA GATCAGCCAA ATGAAAGGCA AGTCCGAATT
    CAGCTGAAGG ATCACATAGA CAGGACAAGG AAGAAGAGAG GAGAAAAAGA TGAAACAGAG
    TATGAATACA GTGGAAGTGA AGAGGAGGAA GAGGAAGTGC CTGAACAGGA AGGAGAACCA
    AGTTCCATTG TTAATGTGCC TGGGGAATCA ACACTCCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGCTC TGAGGCTTTG CGACGGCAAC AGCTGCTGCA GGAGCAACAA
    CTCAGAGAGC AGGAGGAATA TAAAAGACAG CTCCTGGCAG AGAGACAGAA GCGCATAGAA
    CAGCAGAAAG AGCAAAGGAG GAGACTTGAA GAGCAACAAA GAAGAGAGCG TGAGGCTCGT
    CGGCAGCAGG AACGAGAACA AAGAAGGCGA GAGCAAGAGG AAAAGAAGAG AATGGAGGAG
    ATGGAAAGAA GGCGCAAGGA AGAGGAAGAA AGGAGAAGAG CAGAGGAAGA GAAAAGAAGA
    GTGGAAAGAG AGCAGGAGTA TATCCGAAGA CAGCTGGAGG AGGAGCAGCG GCACTTAGAA
    ATCCTTCAGC AGCAGCTGCT TCAGGAGCAG GCCATGTTAC TGGAGTATAG ATGGCGTGAA
    ATGGAGGAGC ACCGCCAGGC TGAAAGACTT CAGCGTCAGT TGCAGCAAGA GCAGGCCTAT
    CTCATGTCAC TACAGCATGA TCATAGACAG CAGCAGCCAC CACAACAGCA GCCCCAGCCC
    CAGCAGCCTC CACTGTCCCA GCAGGAAAGG AGCAAGCAAA CATACCATAC CCAAGAACCC
    AAGCCTCATT ATGAACAAGC AGAACGAATA CGAGAGGCAG AGGAGCGCCT TAGGAAAGCA
    AACCAAGGCT CCCCTGAAGC CCAGGCTAAG CAACCAGGAA GAGTTTTAGA GCCATTAGTG
    CCTTCAAGAT CCGAATCATT TTCCAATGGA AATTCTGAGC CTGCTCAACC TGCCATGCAC
    AGACCAGTGG AACCACAGGT ACAGTGGTCT CATCTGGCAT CCCTCAAGAA CAATGTATCC
    CCTGTCTCGC GATCCCATTC CTTCAGTGAC CCTTCTCCCA AATTTGCACC TCACCTTCTA
    CGATCTCAGG ATTCGCACCA AACTTCACGC AGTGAAGTGC AAAGTCAGGG TTCTGACCCT
    AAGTCTGAAA TCTCTGAGCC AACCCAAAGG GTTTGGGCTC GATCAGACAG TGACGAGGTG
    CCTCCAAGGG TGCCTGTGAG GACAACATCA AGATCTCCAG TATTGTCACG GAAAGATTCA
    CCACTGCAGG GCAGTGGGCA GCAAAATACC CAAGCTGGTC AGAGAAATTC CACAAGCAAT
    ATTGAGCCTA GACTTCTTTG GGAAAGAGTA GAGAAGCTTG TGCCCAGACC GGGTAGTGGT
    AGCTCGTCAG GATCAAGTAA CTCTGGATCC CAAGCAGGAT CCCACCCAGG ATCTCAGAGT
    GGATCTGGAG AGCGCTTCCG GATGAGATCA TCTTCAAAAT CTGAGGGCTC ACCATCTCAA
    CGACATGAAA ACACAGCCAA AAAGTCTGAA GAGAAGAAAG AAGCAGTTAG ACAGGCCAAA
    GCTCCTGGAG AAGTGGATCT TACTGCACTC GCTAAGGAGC TTCGAGCAGT AGATGATGTT
    CGGCCACTTC ACAAAGTTAC TGACTATTCC TCATCCAGTG AAGAGTCTGG TACTACAGAT
    GAGGAGGATG ATGACATGGA ACAGGAAGGA GCAGATGAAT CGACTTCAGG CCCAGAGGAT
    GCTAGAGCTA TGTCATCTTT GAATTTAAGC AATGGAGAAA CGGAGTCTGT GAAAACCATG
    ATCGTACATG ATGATGTCGA AAGTGAACCT GCCATGACCC CATCTAAAGA AGGCACTTTA
    ATTGTCCGAC AGACTCAGTC AGCTAGTAAT ACTCTCCAGA AACACAAATC GTCCTCCTCT
    TTCACACCGT TCATAGATCC AAGGTTACTT CAGATCTCTC CATCTAGTGG AACAACTGTT
    ACGTCAGTAG GTACATACAT GCGCACACTG GAAGCTAACC TTAAAAATAC TAATTTTATT
    TTAGTGGGAT TTTCCAGTGA AGCGCTAAGG CCGGATGCCA TGAGGCAAGA TCCTACAAGG
    AAAGGATCAG TTGTTAATGT TAATCCCACA AATACTCGGC CACAAAGTGA TACACCAGAG
    ATTCGCAAAT ACAAGAAGAG ATTTAATTCT GAGATTTTGT GTGCGGCATT GTGGGGTGTA
    AATTTGCTGG TGGGAACTGA AAGTGGTTTA ATGCTGTTGG ATAGAAGTGG ACAAGGGAAA
    GTGTACCCCC TCATTAACAG AAGGCGATTT CAGCAGATGG ATGTGCTTGA AGGCTTGAAT
    GTCTTGGTAA CTATATCAGG GAAAAAGAAC AAGCTGAGAG TATATTACTT ATCCTGGCTG
    AGGAATAAAA TTTTGCACAA TGATCCTGAA GTAGAGAAGA AACAAGGATG GACAACAGTT
    GGGGATTTGG AAGGCTGTGT GCACTACAAA GTTGTAAAAT ATGAACGTAT CAAGTTTTTG
    GTAATCGCTT TAAAAAGTTC AGTGGAGGTG TATGCCTGGG CACCGAAACC ATACCACAAG
    TTTATGGCCT TTAAGTCATT CGGAGATTTA GTACACAGAC CTCTCCTTGT AGACCTGACT
    GTGGAAGAAG GACAGAGGCT GAAAGTGATC TATGGTTCCT GTGCTGGGTT TCATGCAGTA
    GATGTGGACT CGGGCGCAGT TTATGATATT TACTTGCCAA CACATATTCA GAGCAGCATT
    CAGCCACACG CAATCATCAT TCTCCCAAAT ACTGATGGTA TGGAACTGCT GGTTTGCTAT
    GAAGATGAAG GAGTTTATGT TAATACATAC GGCAGGATCA CCAAAGATGT AGTTCTACAA
    TGGGGAGAAA TGCCTACATC AGTTGCTTAT ATCCGATCTA ACCAGATCAT GGGATGGGGT
    GAAAAAGCTA TTGAGATTCG ATCCGTGGAA ACTGGCCACC TTGATGGAGT ATTTATGCAC
    AAAAGGGCAC AAAGGCTCAA GTTCTTGTGC GAACGAAATG ACAAGGTTTT CTTTGCCTCG
    GTGCGGTCTG GCGGAAGCAG CCAAGTGTAT TTCATGACTT TGGGCAGAAG CTCTCTTCTG
    AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35138
    Clone ID Related Accession (Same CDS sequence) XM_012957670.2 , XM_012957670.3
    Accession Version XM_012957670.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3981bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957670.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCCAACG ATTCGCCTGC CAAAAGTCTT GTGGATATAG ACCTGTCGTC TCTGAGGGAC 
    CCAGCTGGCA TCTTTGAATT AGTGGAAGTG GTAGGAAATG GCACCTATGG ACAAGTATAT
    AAGGGCCGAC ATGTCAAAAC AGGCCAACTT GCAGCCATCA AAGTCATGGA TGTTACAGAG
    GATGAAGAGG AAGAAATCAA ACTGGAAATC AATATGTTGA AGAAATATTC CCACCACAGA
    AATATAGCTA CTTATTATGG TGCTTTTATT AAGAAAAGTC CTCCTGGGCA TGATGACCAG
    CTTTGGTTGG TGATGGAGTT TTGTGGTGCT GGCTCAATCA CTGACCTGGT GAAGAATACA
    AAGGGGAATA GTTTGAAAGA AGACTGGATT GCCTATATCT CTCGTGAGAT ACTTAGGGGA
    TTGGCCCACC TCCATGCTCA TCATGTGATT CACCGGGATA TCAAAGGACA GAATGTGCTG
    CTAACAGAGA ATGCCGAAGT GAAATTAGTG GATTTTGGTG TTAGTGCACA GCTGGATAGG
    ACAGTGGGCC GAAGGAACAC ATTTATTGGT ACACCATACT GGATGGCTCC AGAAGTTATC
    GCCTGTGATG AGAACCCAGA TGCTACCTAT GACTATAGGA GTGACCTCTG GTCATGTGGT
    ATTACAGCGA TTGAAATGGC AGAAGGTGCT CCTCCTCTTT GCGATATGCA TCCAATGCGA
    GCCCTGTTCC TTATCCCAAG AAATCCTCCA CCACGGCTAA AATCCAAGAA ATGGTCAAAG
    AAGTTCTTCA GCTTTATAGA AGGCTGCCTT GTGAAAAATT ACATGCAACG TCCCCCAACA
    GAACAGCTTT TAAAGCATCC ATTTATACGA GATCAGCCAA ATGAAAGGCA AGTCCGAATT
    CAGCTGAAGG ATCACATAGA CAGGACAAGG AAGAAGAGAG GAGAAAAAGA TGAAACAGAG
    TATGAATACA GTGGAAGTGA AGAGGAGGAA GAGGAAGTGC CTGAACAGGA AGGAGAACCA
    AGTTCCATTG TTAATGTGCC TGGGGAATCA ACACTCCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGCTC TGAGGCTTTG CGACGGCAAC AGCTGCTGCA GGAGCAACAA
    CTCAGAGAGC AGGAGGAATA TAAAAGACAG CTCCTGGCAG AGAGACAGAA GCGCATAGAA
    CAGCAGAAAG AGCAAAGGAG GAGACTTGAA GAGCAACAAA GAAGAGAGCG TGAGGCTCGT
    CGGCAGCAGG AACGAGAACA AAGAAGGCGA GAGCAAGAGG AAAAGAAGAG AATGGAGGAG
    ATGGAAAGAA GGCGCAAGGA AGAGGAAGAA AGGAGAAGAG CAGAGGAAGA GAAAAGAAGA
    GTGGAAAGAG AGCAGGAGTA TATCCGAAGA CAGCTGGAGG AGGAGCAGCG GCACTTAGAA
    ATCCTTCAGC AGCAGCTGCT TCAGGAGCAG GCCATGTTAC TGGAGTATAG ATGGCGTGAA
    ATGGAGGAGC ACCGCCAGGC TGAAAGACTT CAGCGTCAGT TGCAGCAAGA GCAGGCCTAT
    CTCATGTCAC TACAGCATGA TCATAGACAG CAGCAGCCAC CACAACAGCA GCCCCAGCCC
    CAGCAGCCTC CACTGTCCCA GCAGGAAAGG AGCAAGCAAA CATACCATAC CCAAGAACCC
    AAGCCTCATT ATGAACAAGC AGAACGAATA CGAGAGGCAG AGGAGCGCCT TAGGAAAGCA
    AACCAAGGCT CCCCTGAAGC CCAGGCTAAG CAACCAGGAA GAGTTTTAGA GCCATTAGTG
    CCTTCAAGAT CCGAATCATT TTCCAATGGA AATTCTGAGC CTGCTCAACC TGCCATGCAC
    AGACCAGTGG AACCACAGGT GCCTGTGAGG ACAACATCAA GATCTCCAGT ATTGTCACGG
    AAAGATTCAC CACTGCAGGG CAGTGGGCAG CAAAATACCC AAGCTGGTCA GAGAAATTCC
    ACAAGCAATA TTGAGCCTAG ACTTCTTTGG GAAAGAGTAG AGAAGCTTGT GCCCAGACCG
    GGTAGTGGTA GCTCGTCAGG ATCAAGTAAC TCTGGATCCC AAGCAGGATC CCACCCAGGA
    TCTCAGAGTG GATCTGGAGA GCGCTTCCGG ATGAGATCAT CTTCAAAATC TGAGGGCTCA
    CCATCTCAAC GACATGAAAA CACAGCCAAA AAGTCTGAAG AGAAGAAAGA AGCAGTTAGA
    CAGGCCAAAG CTCCTGGAGA AGTGGATCTT ACTGCACTCG CTAAGGAGCT TCGAGCAGTA
    GATGATGTTC GGCCACTTCA CAAAGTTACT GACTATTCCT CATCCAGTGA AGAGTCTGGT
    ACTACAGATG AGGAGGATGA TGACATGGAA CAGGAAGGAG CAGATGAATC GACTTCAGGC
    CCAGAGGATG CTAGAGCTAT GTCATCTTTG AATTTAAGCA ATGGAGAAAC GGAGTCTGTG
    AAAACCATGA TCGTACATGA TGATGTCGAA AGTGAACCTG CCATGACCCC ATCTAAAGAA
    GGCACTTTAA TTGTCCGACA GAGTACAGTT GACAAAAAGC GTGCCAACCA ACTTGAGAGC
    AATGGCTTTG CGAGTCGTAT TCACCTCTTG CCAGATCTCT TACAGCAAAG CCATTCCTCC
    TCCACCACTT CTTCCCCAAC CTCAAGTCAA CCGACACCTA CCATGTCCCC CCAGACACCC
    CAGGACAAGC TAACTGCTAA TGAGACTCAG TCAGCTAGTA ATACTCTCCA GAAACACAAA
    TCGTCCTCCT CTTTCACACC GTTCATAGAT CCAAGGTTAC TTCAGATCTC TCCATCTAGT
    GGAACAACTG TTACGTCAGT AGGTACATAC ATGCGCACAC TGGAAGCTAA CCTTAAAAAT
    ACTAATTTTA TTTTAGTGGG ATTTTCCAGT GAAGCGCTAA GGCCGGATGC CATGAGGCAA
    GATCCTACAA GGAAAGGATC AGTTGTTAAT GTTAATCCCA CAAATACTCG GCCACAAAGT
    GATACACCAG AGATTCGCAA ATACAAGAAG AGATTTAATT CTGAGATTTT GTGTGCGGCA
    TTGTGGGGTG TAAATTTGCT GGTGGGAACT GAAAGTGGTT TAATGCTGTT GGATAGAAGT
    GGACAAGGGA AAGTGTACCC CCTCATTAAC AGAAGGCGAT TTCAGCAGAT GGATGTGCTT
    GAAGGCTTGA ATGTCTTGGT AACTATATCA GGGAAAAAGA ACAAGCTGAG AGTATATTAC
    TTATCCTGGC TGAGGAATAA AATTTTGCAC AATGATCCTG AAGTAGAGAA GAAACAAGGA
    TGGACAACAG TTGGGGATTT GGAAGGCTGT GTGCACTACA AAGTTGTAAA ATATGAACGT
    ATCAAGTTTT TGGTAATCGC TTTAAAAAGT TCAGTGGAGG TGTATGCCTG GGCACCGAAA
    CCATACCACA AGTTTATGGC CTTTAAGTCA TTCGGAGATT TAGTACACAG ACCTCTCCTT
    GTAGACCTGA CTGTGGAAGA AGGACAGAGG CTGAAAGTGA TCTATGGTTC CTGTGCTGGG
    TTTCATGCAG TAGATGTGGA CTCGGGCGCA GTTTATGATA TTTACTTGCC AACACATATT
    CAGAGCAGCA TTCAGCCACA CGCAATCATC ATTCTCCCAA ATACTGATGG TATGGAACTG
    CTGGTTTGCT ATGAAGATGA AGGAGTTTAT GTTAATACAT ACGGCAGGAT CACCAAAGAT
    GTAGTTCTAC AATGGGGAGA AATGCCTACA TCAGTTGCTT ATATCCGATC TAACCAGATC
    ATGGGATGGG GTGAAAAAGC TATTGAGATT CGATCCGTGG AAACTGGCCA CCTTGATGGA
    GTATTTATGC ACAAAAGGGC ACAAAGGCTC AAGTTCTTGT GCGAACGAAA TGACAAGGTT
    TTCTTTGCCT CGGTGCGGTC TGGCGGAAGC AGCCAAGTGT ATTTCATGAC TTTGGGCAGA
    AGCTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813124.1
    CDS344..4324
    Translation

    Target ORF information:

    RefSeq Version XM_012957670.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 4 (map4k4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957670.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCCAACG ATTCGCCTGC CAAAAGTCTT GTGGATATAG ACCTGTCGTC TCTGAGGGAC 
    CCAGCTGGCA TCTTTGAATT AGTGGAAGTG GTAGGAAATG GCACCTATGG ACAAGTATAT
    AAGGGCCGAC ATGTCAAAAC AGGCCAACTT GCAGCCATCA AAGTCATGGA TGTTACAGAG
    GATGAAGAGG AAGAAATCAA ACTGGAAATC AATATGTTGA AGAAATATTC CCACCACAGA
    AATATAGCTA CTTATTATGG TGCTTTTATT AAGAAAAGTC CTCCTGGGCA TGATGACCAG
    CTTTGGTTGG TGATGGAGTT TTGTGGTGCT GGCTCAATCA CTGACCTGGT GAAGAATACA
    AAGGGGAATA GTTTGAAAGA AGACTGGATT GCCTATATCT CTCGTGAGAT ACTTAGGGGA
    TTGGCCCACC TCCATGCTCA TCATGTGATT CACCGGGATA TCAAAGGACA GAATGTGCTG
    CTAACAGAGA ATGCCGAAGT GAAATTAGTG GATTTTGGTG TTAGTGCACA GCTGGATAGG
    ACAGTGGGCC GAAGGAACAC ATTTATTGGT ACACCATACT GGATGGCTCC AGAAGTTATC
    GCCTGTGATG AGAACCCAGA TGCTACCTAT GACTATAGGA GTGACCTCTG GTCATGTGGT
    ATTACAGCGA TTGAAATGGC AGAAGGTGCT CCTCCTCTTT GCGATATGCA TCCAATGCGA
    GCCCTGTTCC TTATCCCAAG AAATCCTCCA CCACGGCTAA AATCCAAGAA ATGGTCAAAG
    AAGTTCTTCA GCTTTATAGA AGGCTGCCTT GTGAAAAATT ACATGCAACG TCCCCCAACA
    GAACAGCTTT TAAAGCATCC ATTTATACGA GATCAGCCAA ATGAAAGGCA AGTCCGAATT
    CAGCTGAAGG ATCACATAGA CAGGACAAGG AAGAAGAGAG GAGAAAAAGA TGAAACAGAG
    TATGAATACA GTGGAAGTGA AGAGGAGGAA GAGGAAGTGC CTGAACAGGA AGGAGAACCA
    AGTTCCATTG TTAATGTGCC TGGGGAATCA ACACTCCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGCTC TGAGGCTTTG CGACGGCAAC AGCTGCTGCA GGAGCAACAA
    CTCAGAGAGC AGGAGGAATA TAAAAGACAG CTCCTGGCAG AGAGACAGAA GCGCATAGAA
    CAGCAGAAAG AGCAAAGGAG GAGACTTGAA GAGCAACAAA GAAGAGAGCG TGAGGCTCGT
    CGGCAGCAGG AACGAGAACA AAGAAGGCGA GAGCAAGAGG AAAAGAAGAG AATGGAGGAG
    ATGGAAAGAA GGCGCAAGGA AGAGGAAGAA AGGAGAAGAG CAGAGGAAGA GAAAAGAAGA
    GTGGAAAGAG AGCAGGAGTA TATCCGAAGA CAGCTGGAGG AGGAGCAGCG GCACTTAGAA
    ATCCTTCAGC AGCAGCTGCT TCAGGAGCAG GCCATGTTAC TGGAGTATAG ATGGCGTGAA
    ATGGAGGAGC ACCGCCAGGC TGAAAGACTT CAGCGTCAGT TGCAGCAAGA GCAGGCCTAT
    CTCATGTCAC TACAGCATGA TCATAGACAG CAGCAGCCAC CACAACAGCA GCCCCAGCCC
    CAGCAGCCTC CACTGTCCCA GCAGGAAAGG AGCAAGCAAA CATACCATAC CCAAGAACCC
    AAGCCTCATT ATGAACAAGC AGAACGAATA CGAGAGGCAG AGGAGCGCCT TAGGAAAGCA
    AACCAAGGCT CCCCTGAAGC CCAGGCTAAG CAACCAGGAA GAGTTTTAGA GCCATTAGTG
    CCTTCAAGAT CCGAATCATT TTCCAATGGA AATTCTGAGC CTGCTCAACC TGCCATGCAC
    AGACCAGTGG AACCACAGGT GCCTGTGAGG ACAACATCAA GATCTCCAGT ATTGTCACGG
    AAAGATTCAC CACTGCAGGG CAGTGGGCAG CAAAATACCC AAGCTGGTCA GAGAAATTCC
    ACAAGCAATA TTGAGCCTAG ACTTCTTTGG GAAAGAGTAG AGAAGCTTGT GCCCAGACCG
    GGTAGTGGTA GCTCGTCAGG ATCAAGTAAC TCTGGATCCC AAGCAGGATC CCACCCAGGA
    TCTCAGAGTG GATCTGGAGA GCGCTTCCGG ATGAGATCAT CTTCAAAATC TGAGGGCTCA
    CCATCTCAAC GACATGAAAA CACAGCCAAA AAGTCTGAAG AGAAGAAAGA AGCAGTTAGA
    CAGGCCAAAG CTCCTGGAGA AGTGGATCTT ACTGCACTCG CTAAGGAGCT TCGAGCAGTA
    GATGATGTTC GGCCACTTCA CAAAGTTACT GACTATTCCT CATCCAGTGA AGAGTCTGGT
    ACTACAGATG AGGAGGATGA TGACATGGAA CAGGAAGGAG CAGATGAATC GACTTCAGGC
    CCAGAGGATG CTAGAGCTAT GTCATCTTTG AATTTAAGCA ATGGAGAAAC GGAGTCTGTG
    AAAACCATGA TCGTACATGA TGATGTCGAA AGTGAACCTG CCATGACCCC ATCTAAAGAA
    GGCACTTTAA TTGTCCGACA GAGTACAGTT GACAAAAAGC GTGCCAACCA ACTTGAGAGC
    AATGGCTTTG CGAGTCGTAT TCACCTCTTG CCAGATCTCT TACAGCAAAG CCATTCCTCC
    TCCACCACTT CTTCCCCAAC CTCAAGTCAA CCGACACCTA CCATGTCCCC CCAGACACCC
    CAGGACAAGC TAACTGCTAA TGAGACTCAG TCAGCTAGTA ATACTCTCCA GAAACACAAA
    TCGTCCTCCT CTTTCACACC GTTCATAGAT CCAAGGTTAC TTCAGATCTC TCCATCTAGT
    GGAACAACTG TTACGTCAGT AGGTACATAC ATGCGCACAC TGGAAGCTAA CCTTAAAAAT
    ACTAATTTTA TTTTAGTGGG ATTTTCCAGT GAAGCGCTAA GGCCGGATGC CATGAGGCAA
    GATCCTACAA GGAAAGGATC AGTTGTTAAT GTTAATCCCA CAAATACTCG GCCACAAAGT
    GATACACCAG AGATTCGCAA ATACAAGAAG AGATTTAATT CTGAGATTTT GTGTGCGGCA
    TTGTGGGGTG TAAATTTGCT GGTGGGAACT GAAAGTGGTT TAATGCTGTT GGATAGAAGT
    GGACAAGGGA AAGTGTACCC CCTCATTAAC AGAAGGCGAT TTCAGCAGAT GGATGTGCTT
    GAAGGCTTGA ATGTCTTGGT AACTATATCA GGGAAAAAGA ACAAGCTGAG AGTATATTAC
    TTATCCTGGC TGAGGAATAA AATTTTGCAC AATGATCCTG AAGTAGAGAA GAAACAAGGA
    TGGACAACAG TTGGGGATTT GGAAGGCTGT GTGCACTACA AAGTTGTAAA ATATGAACGT
    ATCAAGTTTT TGGTAATCGC TTTAAAAAGT TCAGTGGAGG TGTATGCCTG GGCACCGAAA
    CCATACCACA AGTTTATGGC CTTTAAGTCA TTCGGAGATT TAGTACACAG ACCTCTCCTT
    GTAGACCTGA CTGTGGAAGA AGGACAGAGG CTGAAAGTGA TCTATGGTTC CTGTGCTGGG
    TTTCATGCAG TAGATGTGGA CTCGGGCGCA GTTTATGATA TTTACTTGCC AACACATATT
    CAGAGCAGCA TTCAGCCACA CGCAATCATC ATTCTCCCAA ATACTGATGG TATGGAACTG
    CTGGTTTGCT ATGAAGATGA AGGAGTTTAT GTTAATACAT ACGGCAGGAT CACCAAAGAT
    GTAGTTCTAC AATGGGGAGA AATGCCTACA TCAGTTGCTT ATATCCGATC TAACCAGATC
    ATGGGATGGG GTGAAAAAGC TATTGAGATT CGATCCGTGG AAACTGGCCA CCTTGATGGA
    GTATTTATGC ACAAAAGGGC ACAAAGGCTC AAGTTCTTGT GCGAACGAAA TGACAAGGTT
    TTCTTTGCCT CGGTGCGGTC TGGCGGAAGC AGCCAAGTGT ATTTCATGAC TTTGGGCAGA
    AGCTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35139
    Clone ID Related Accession (Same CDS sequence) XM_012957671.2 , XM_012957671.3
    Accession Version XM_012957671.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3975bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957671.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCCAACG ATTCGCCTGC CAAAAGTCTT GTGGATATAG ACCTGTCGTC TCTGAGGGAC 
    CCAGCTGGCA TCTTTGAATT AGTGGAAGTG GTAGGAAATG GCACCTATGG ACAAGTATAT
    AAGGGCCGAC ATGTCAAAAC AGGCCAACTT GCAGCCATCA AAGTCATGGA TGTTACAGAG
    GATGAAGAGG AAGAAATCAA ACTGGAAATC AATATGTTGA AGAAATATTC CCACCACAGA
    AATATAGCTA CTTATTATGG TGCTTTTATT AAGAAAAGTC CTCCTGGGCA TGATGACCAG
    CTTTGGTTGG TGATGGAGTT TTGTGGTGCT GGCTCAATCA CTGACCTGGT GAAGAATACA
    AAGGGGAATA GTTTGAAAGA AGACTGGATT GCCTATATCT CTCGTGAGAT ACTTAGGGGA
    TTGGCCCACC TCCATGCTCA TCATGTGATT CACCGGGATA TCAAAGGACA GAATGTGCTG
    CTAACAGAGA ATGCCGAAGT GAAATTAGTG GATTTTGGTG TTAGTGCACA GCTGGATAGG
    ACAGTGGGCC GAAGGAACAC ATTTATTGGT ACACCATACT GGATGGCTCC AGAAGTTATC
    GCCTGTGATG AGAACCCAGA TGCTACCTAT GACTATAGGA GTGACCTCTG GTCATGTGGT
    ATTACAGCGA TTGAAATGGC AGAAGGTGCT CCTCCTCTTT GCGATATGCA TCCAATGCGA
    GCCCTGTTCC TTATCCCAAG AAATCCTCCA CCACGGCTAA AATCCAAGAA ATGGTCAAAG
    AAGTTCTTCA GCTTTATAGA AGGCTGCCTT GTGAAAAATT ACATGCAACG TCCCCCAACA
    GAACAGCTTT TAAAGCATCC ATTTATACGA GATCAGCCAA ATGAAAGGCA AGTCCGAATT
    CAGCTGAAGG ATCACATAGA CAGGACAAGG AAGAAGAGAG GAGAAAAAGA TGAAACAGAG
    TATGAATACA GTGGAAGTGA AGAGGAGGAA GAGGAAGTGC CTGAACAGGA AGGAGAACCA
    AGTTCCATTG TTAATGTGCC TGGGGAATCA ACACTCCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGCTC TGAGGCTTTG CGACGGCAAC AGCTGCTGCA GGAGCAACAA
    CTCAGAGAGC AGGAGGAATA TAAAAGACAG CTCCTGGCAG AGAGACAGAA GCGCATAGAA
    CAGCAGAAAG AGCAAAGGAG GAGACTTGAA GAGCAACAAA GAAGAGAGCG TGAGGCTCGT
    CGGCAGCAGG AACGAGAACA AAGAAGGCGA GAGCAAGAGG AAAAGAAGAG AATGGAGGAG
    ATGGAAAGAA GGCGCAAGGA AGAGGAAGAA AGGAGAAGAG CAGAGGAAGA GAAAAGAAGA
    GTGGAAAGAG AGCAGGAGTA TATCCGAAGA CAGCTGGAGG AGGAGCAGCG GCACTTAGAA
    ATCCTTCAGC AGCAGCTGCT TCAGGAGCAG GCCATGTTAC TGGAGTATAG ATGGCGTGAA
    ATGGAGGAGC ACCGCCAGGC TGAAAGACTT CAGCGTCAGT TGCAGCAAGA GCAGGCCTAT
    CTCATGTCAC TACAGCATGA TCATAGACAG CAGCAGCCAC CACAACAGCA GCCCCAGCCC
    CAGCAGCCTC CACTGTCCCA GCAGGAAAGG AGCAAGCAAA CATACCATAC CCAAGAACCC
    AAGCCTCATT ATGAACAAGC AGAACGAATA CGAGAGGCAG AGGAGCGCCT TAGGAAAGCA
    AACCAAGGCT CCCCTGAAGC CCAGGCTAAG CAACCAGGAA GAGTTTTAGA GCCATTAGTG
    CCTTCAAGAT CCGAATCATT TTCCAATGGA AATTCTGAGC CTGCTCAACC TGCCATGCAC
    AGACCAGTGG AACCACAGGT ACAGTGGTCT CATCTGGCAT CCCTCAAGAA CAATGTATCC
    CCTGTCTCGC GATCCCATTC CTTCAGTGAC CCTTCTCCCA AATTTGCACC TCACCTTCTA
    CGATCTCAGG ATTCGCACCA AACTTCACGC AGTGAAGTGC AAAGTCAGGG TTCTGACCCT
    AAGTCTGAAA TCTCTGAGCC AACCCAAAGG GTTTGGGCTC GATCAGACAG TGACGAGGTG
    CCTCCAAGGG TGCCTGTGAG GACAACATCA AGATCTCCAG TATTGTCACG GAAAGATTCA
    CCACTGCAGG GCAGTGGGCA GCAAAATACC CAAGCTGGTC AGAGAAATTC CACAAGCAAT
    ATTGAGCCTA GACTTCTTTG GGAAAGAGTA GAGAAGCTTG TGCCCAGACC GGGTAGTGGT
    AGCTCGTCAG GATCAAGTAA CTCTGGATCC CAAGCAGGAT CCCACCCAGG ATCTCAGAGT
    GGATCTGGAG AGCGCTTCCG GATGAGATCA TCTTCAAAAT CTGAGGGCTC ACCATCTCAA
    CGACATGAAA ACACAGCCAA AAAGTCTGAA GAGAAGAAAG AAGCAGTTAG ACAGGCCAAA
    GCTCCTGGAG AAGTGGATCT TACTGCACTC GCTAAGGAGC TTCGAGCAGT AGATGATGTT
    CGGCCACTTC ACAAAGTTAC TGACTATTCC TCATCCAGTG AAGAGTCTGG TACTACAGAT
    GAGGAGGATG ATGACATGGA ACAGGAAGGA GCAGATGAAT CGACTTCAGG CCCAGAGGAT
    GCTAGAGCTA TGTCATCTTT GAATTTAAGC AATGGAGAAA CGGAGTCTGT GAAAACCATG
    ATCGTACATG ATGATGTCGA AAGTGAACCT GCCATGACCC CATCTAAAGA AGGCACTTTA
    ATTGTCCGAC AGACTCAGTC AGCTAGTAAT ACTCTCCAGA AACACAAATC GTCCTCCTCT
    TTCACACCGT TCATAGATCC AAGGTTACTT CAGATCTCTC CATCTAGTGG AACAACTGTT
    ACGTCAGTAG TGGGATTTTC CAGTGAAGCG CTAAGGCCGG ATGCCATGAG GCAAGATCCT
    ACAAGGAAAG GATCAGTTGT TAATGTTAAT CCCACAAATA CTCGGCCACA AAGTGATACA
    CCAGAGATTC GCAAATACAA GAAGAGATTT AATTCTGAGA TTTTGTGTGC GGCATTGTGG
    GGTGTAAATT TGCTGGTGGG AACTGAAAGT GGTTTAATGC TGTTGGATAG AAGTGGACAA
    GGGAAAGTGT ACCCCCTCAT TAACAGAAGG CGATTTCAGC AGATGGATGT GCTTGAAGGC
    TTGAATGTCT TGGTAACTAT ATCAGGGAAA AAGAACAAGC TGAGAGTATA TTACTTATCC
    TGGCTGAGGA ATAAAATTTT GCACAATGAT CCTGAAGTAG AGAAGAAACA AGGATGGACA
    ACAGTTGGGG ATTTGGAAGG CTGTGTGCAC TACAAAGTTG TAAAATATGA ACGTATCAAG
    TTTTTGGTAA TCGCTTTAAA AAGTTCAGTG GAGGTGTATG CCTGGGCACC GAAACCATAC
    CACAAGTTTA TGGCCTTTAA GTCATTCGGA GATTTAGTAC ACAGACCTCT CCTTGTAGAC
    CTGACTGTGG AAGAAGGACA GAGGCTGAAA GTGATCTATG GTTCCTGTGC TGGGTTTCAT
    GCAGTAGATG TGGACTCGGG CGCAGTTTAT GATATTTACT TGCCAACACA TATTCAGAGC
    AGCATTCAGC CACACGCAAT CATCATTCTC CCAAATACTG ATGGTATGGA ACTGCTGGTT
    TGCTATGAAG ATGAAGGAGT TTATGTTAAT ACATACGGCA GGATCACCAA AGATGTAGTT
    CTACAATGGG GAGAAATGCC TACATCAGTT GCTTATATCC GATCTAACCA GATCATGGGA
    TGGGGTGAAA AAGCTATTGA GATTCGATCC GTGGAAACTG GCCACCTTGA TGGAGTATTT
    ATGCACAAAA GGGCACAAAG GCTCAAGTTC TTGTGCGAAC GAAATGACAA GGTTTTCTTT
    GCCTCGGTGC GGTCTGGCGG AAGCAGCCAA GTGTATTTCA TGACTTTGGG CAGAAGCTCT
    CTTCTGAGCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813125.1
    CDS344..4318
    Translation

    Target ORF information:

    RefSeq Version XM_012957671.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 4 (map4k4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957671.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCCAACG ATTCGCCTGC CAAAAGTCTT GTGGATATAG ACCTGTCGTC TCTGAGGGAC 
    CCAGCTGGCA TCTTTGAATT AGTGGAAGTG GTAGGAAATG GCACCTATGG ACAAGTATAT
    AAGGGCCGAC ATGTCAAAAC AGGCCAACTT GCAGCCATCA AAGTCATGGA TGTTACAGAG
    GATGAAGAGG AAGAAATCAA ACTGGAAATC AATATGTTGA AGAAATATTC CCACCACAGA
    AATATAGCTA CTTATTATGG TGCTTTTATT AAGAAAAGTC CTCCTGGGCA TGATGACCAG
    CTTTGGTTGG TGATGGAGTT TTGTGGTGCT GGCTCAATCA CTGACCTGGT GAAGAATACA
    AAGGGGAATA GTTTGAAAGA AGACTGGATT GCCTATATCT CTCGTGAGAT ACTTAGGGGA
    TTGGCCCACC TCCATGCTCA TCATGTGATT CACCGGGATA TCAAAGGACA GAATGTGCTG
    CTAACAGAGA ATGCCGAAGT GAAATTAGTG GATTTTGGTG TTAGTGCACA GCTGGATAGG
    ACAGTGGGCC GAAGGAACAC ATTTATTGGT ACACCATACT GGATGGCTCC AGAAGTTATC
    GCCTGTGATG AGAACCCAGA TGCTACCTAT GACTATAGGA GTGACCTCTG GTCATGTGGT
    ATTACAGCGA TTGAAATGGC AGAAGGTGCT CCTCCTCTTT GCGATATGCA TCCAATGCGA
    GCCCTGTTCC TTATCCCAAG AAATCCTCCA CCACGGCTAA AATCCAAGAA ATGGTCAAAG
    AAGTTCTTCA GCTTTATAGA AGGCTGCCTT GTGAAAAATT ACATGCAACG TCCCCCAACA
    GAACAGCTTT TAAAGCATCC ATTTATACGA GATCAGCCAA ATGAAAGGCA AGTCCGAATT
    CAGCTGAAGG ATCACATAGA CAGGACAAGG AAGAAGAGAG GAGAAAAAGA TGAAACAGAG
    TATGAATACA GTGGAAGTGA AGAGGAGGAA GAGGAAGTGC CTGAACAGGA AGGAGAACCA
    AGTTCCATTG TTAATGTGCC TGGGGAATCA ACACTCCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGCTC TGAGGCTTTG CGACGGCAAC AGCTGCTGCA GGAGCAACAA
    CTCAGAGAGC AGGAGGAATA TAAAAGACAG CTCCTGGCAG AGAGACAGAA GCGCATAGAA
    CAGCAGAAAG AGCAAAGGAG GAGACTTGAA GAGCAACAAA GAAGAGAGCG TGAGGCTCGT
    CGGCAGCAGG AACGAGAACA AAGAAGGCGA GAGCAAGAGG AAAAGAAGAG AATGGAGGAG
    ATGGAAAGAA GGCGCAAGGA AGAGGAAGAA AGGAGAAGAG CAGAGGAAGA GAAAAGAAGA
    GTGGAAAGAG AGCAGGAGTA TATCCGAAGA CAGCTGGAGG AGGAGCAGCG GCACTTAGAA
    ATCCTTCAGC AGCAGCTGCT TCAGGAGCAG GCCATGTTAC TGGAGTATAG ATGGCGTGAA
    ATGGAGGAGC ACCGCCAGGC TGAAAGACTT CAGCGTCAGT TGCAGCAAGA GCAGGCCTAT
    CTCATGTCAC TACAGCATGA TCATAGACAG CAGCAGCCAC CACAACAGCA GCCCCAGCCC
    CAGCAGCCTC CACTGTCCCA GCAGGAAAGG AGCAAGCAAA CATACCATAC CCAAGAACCC
    AAGCCTCATT ATGAACAAGC AGAACGAATA CGAGAGGCAG AGGAGCGCCT TAGGAAAGCA
    AACCAAGGCT CCCCTGAAGC CCAGGCTAAG CAACCAGGAA GAGTTTTAGA GCCATTAGTG
    CCTTCAAGAT CCGAATCATT TTCCAATGGA AATTCTGAGC CTGCTCAACC TGCCATGCAC
    AGACCAGTGG AACCACAGGT ACAGTGGTCT CATCTGGCAT CCCTCAAGAA CAATGTATCC
    CCTGTCTCGC GATCCCATTC CTTCAGTGAC CCTTCTCCCA AATTTGCACC TCACCTTCTA
    CGATCTCAGG ATTCGCACCA AACTTCACGC AGTGAAGTGC AAAGTCAGGG TTCTGACCCT
    AAGTCTGAAA TCTCTGAGCC AACCCAAAGG GTTTGGGCTC GATCAGACAG TGACGAGGTG
    CCTCCAAGGG TGCCTGTGAG GACAACATCA AGATCTCCAG TATTGTCACG GAAAGATTCA
    CCACTGCAGG GCAGTGGGCA GCAAAATACC CAAGCTGGTC AGAGAAATTC CACAAGCAAT
    ATTGAGCCTA GACTTCTTTG GGAAAGAGTA GAGAAGCTTG TGCCCAGACC GGGTAGTGGT
    AGCTCGTCAG GATCAAGTAA CTCTGGATCC CAAGCAGGAT CCCACCCAGG ATCTCAGAGT
    GGATCTGGAG AGCGCTTCCG GATGAGATCA TCTTCAAAAT CTGAGGGCTC ACCATCTCAA
    CGACATGAAA ACACAGCCAA AAAGTCTGAA GAGAAGAAAG AAGCAGTTAG ACAGGCCAAA
    GCTCCTGGAG AAGTGGATCT TACTGCACTC GCTAAGGAGC TTCGAGCAGT AGATGATGTT
    CGGCCACTTC ACAAAGTTAC TGACTATTCC TCATCCAGTG AAGAGTCTGG TACTACAGAT
    GAGGAGGATG ATGACATGGA ACAGGAAGGA GCAGATGAAT CGACTTCAGG CCCAGAGGAT
    GCTAGAGCTA TGTCATCTTT GAATTTAAGC AATGGAGAAA CGGAGTCTGT GAAAACCATG
    ATCGTACATG ATGATGTCGA AAGTGAACCT GCCATGACCC CATCTAAAGA AGGCACTTTA
    ATTGTCCGAC AGACTCAGTC AGCTAGTAAT ACTCTCCAGA AACACAAATC GTCCTCCTCT
    TTCACACCGT TCATAGATCC AAGGTTACTT CAGATCTCTC CATCTAGTGG AACAACTGTT
    ACGTCAGTAG TGGGATTTTC CAGTGAAGCG CTAAGGCCGG ATGCCATGAG GCAAGATCCT
    ACAAGGAAAG GATCAGTTGT TAATGTTAAT CCCACAAATA CTCGGCCACA AAGTGATACA
    CCAGAGATTC GCAAATACAA GAAGAGATTT AATTCTGAGA TTTTGTGTGC GGCATTGTGG
    GGTGTAAATT TGCTGGTGGG AACTGAAAGT GGTTTAATGC TGTTGGATAG AAGTGGACAA
    GGGAAAGTGT ACCCCCTCAT TAACAGAAGG CGATTTCAGC AGATGGATGT GCTTGAAGGC
    TTGAATGTCT TGGTAACTAT ATCAGGGAAA AAGAACAAGC TGAGAGTATA TTACTTATCC
    TGGCTGAGGA ATAAAATTTT GCACAATGAT CCTGAAGTAG AGAAGAAACA AGGATGGACA
    ACAGTTGGGG ATTTGGAAGG CTGTGTGCAC TACAAAGTTG TAAAATATGA ACGTATCAAG
    TTTTTGGTAA TCGCTTTAAA AAGTTCAGTG GAGGTGTATG CCTGGGCACC GAAACCATAC
    CACAAGTTTA TGGCCTTTAA GTCATTCGGA GATTTAGTAC ACAGACCTCT CCTTGTAGAC
    CTGACTGTGG AAGAAGGACA GAGGCTGAAA GTGATCTATG GTTCCTGTGC TGGGTTTCAT
    GCAGTAGATG TGGACTCGGG CGCAGTTTAT GATATTTACT TGCCAACACA TATTCAGAGC
    AGCATTCAGC CACACGCAAT CATCATTCTC CCAAATACTG ATGGTATGGA ACTGCTGGTT
    TGCTATGAAG ATGAAGGAGT TTATGTTAAT ACATACGGCA GGATCACCAA AGATGTAGTT
    CTACAATGGG GAGAAATGCC TACATCAGTT GCTTATATCC GATCTAACCA GATCATGGGA
    TGGGGTGAAA AAGCTATTGA GATTCGATCC GTGGAAACTG GCCACCTTGA TGGAGTATTT
    ATGCACAAAA GGGCACAAAG GCTCAAGTTC TTGTGCGAAC GAAATGACAA GGTTTTCTTT
    GCCTCGGTGC GGTCTGGCGG AAGCAGCCAA GTGTATTTCA TGACTTTGGG CAGAAGCTCT
    CTTCTGAGCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35140
    Clone ID Related Accession (Same CDS sequence) XM_012957672.3 , XM_012957672.2
    Accession Version XM_012957672.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3744bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957672.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCCAACG ATTCGCCTGC CAAAAGTCTT GTGGATATAG ACCTGTCGTC TCTGAGGGAC 
    CCAGCTGGCA TCTTTGAATT AGTGGAAGTG GTAGGAAATG GCACCTATGG ACAAGTATAT
    AAGGGCCGAC ATGTCAAAAC AGGCCAACTT GCAGCCATCA AAGTCATGGA TGTTACAGAG
    GATGAAGAGG AAGAAATCAA ACTGGAAATC AATATGTTGA AGAAATATTC CCACCACAGA
    AATATAGCTA CTTATTATGG TGCTTTTATT AAGAAAAGTC CTCCTGGGCA TGATGACCAG
    CTTTGGTTGG TGATGGAGTT TTGTGGTGCT GGCTCAATCA CTGACCTGGT GAAGAATACA
    AAGGGGAATA GTTTGAAAGA AGACTGGATT GCCTATATCT CTCGTGAGAT ACTTAGGGGA
    TTGGCCCACC TCCATGCTCA TCATGTGATT CACCGGGATA TCAAAGGACA GAATGTGCTG
    CTAACAGAGA ATGCCGAAGT GAAATTAGTG GATTTTGGTG TTAGTGCACA GCTGGATAGG
    ACAGTGGGCC GAAGGAACAC ATTTATTGGT ACACCATACT GGATGGCTCC AGAAGTTATC
    GCCTGTGATG AGAACCCAGA TGCTACCTAT GACTATAGGA GTGACCTCTG GTCATGTGGT
    ATTACAGCGA TTGAAATGGC AGAAGGTGCT CCTCCTCTTT GCGATATGCA TCCAATGCGA
    GCCCTGTTCC TTATCCCAAG AAATCCTCCA CCACGGCTAA AATCCAAGAA ATGGTCAAAG
    AAGTTCTTCA GCTTTATAGA AGGCTGCCTT GTGAAAAATT ACATGCAACG TCCCCCAACA
    GAACAGCTTT TAAAGCATCC ATTTATACGA GATCAGCCAA ATGAAAGGCA AGTCCGAATT
    CAGCTGAAGG ATCACATAGA CAGGACAAGG AAGAAGAGAG GAGAAAAAGA TGAAACAGAG
    TATGAATACA GTGGAAGTGA AGAGGAGGAA GAGGAAGTGC CTGAACAGGA AGGAGAACCA
    AGTTCCATTG TTAATGTGCC TGGGGAATCA ACACTCCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGCTC TGAGGCTTTG CGACGGCAAC AGCTGCTGCA GGAGCAACAA
    CTCAGAGAGC AGGAGGAATA TAAAAGACAG CTCCTGGCAG AGAGACAGAA GCGCATAGAA
    CAGCAGAAAG AGCAAAGGAG GAGACTTGAA GAGCAACAAA GAAGAGAGCG TGAGGCTCGT
    CGGCAGCAGG AACGAGAACA AAGAAGGCGA GAGCAAGAGG AAAAGAAGAG AATGGAGGAG
    ATGGAAAGAA GGCGCAAGGA AGAGGAAGAA AGGAGAAGAG CAGAGGAAGA GAAAAGAAGA
    GTGGAAAGAG AGCAGGAGTA TATCCGAAGA CAGCTGGAGG AGGAGCAGCG GCACTTAGAA
    ATCCTTCAGC AGCAGCTGCT TCAGGAGCAG GCCATGTTAC TGGAGTATAG ATGGCGTGAA
    ATGGAGGAGC ACCGCCAGGC TGAAAGACTT CAGCGTCAGT TGCAGCAAGA GCAGGCCTAT
    CTCATGTCAC TACAGCATGA TCATAGACAG CAGCAGCCAC CACAACAGCA GCCCCAGCCC
    CAGCAGCCTC CACTGTCCCA GCAGGAAAGG AGCAAGCAAA CATACCATAC CCAAGAACCC
    AAGCCTCATT ATGAACAAGC AGAACGAATA CGAGAGGCAG AGGAGCGCCT TAGGAAAGCA
    AACCAAGGCT CCCCTGAAGC CCAGGCTAAG CAACCAGGAA GAGTTTTAGA GCCATTAGTG
    CCTTCAAGAT CCGAATCATT TTCCAATGGA AATTCTGAGC CTGCTCAACC TGCCATGCAC
    AGACCAGTGG AACCACAGGT GCCTGTGAGG ACAACATCAA GATCTCCAGT ATTGTCACGG
    AAAGATTCAC CACTGCAGGG CAGTGGGCAG CAAAATACCC AAGCTGGTCA GAGAAATTCC
    ACAAGCAATA TTGAGCCTAG ACTTCTTTGG GAAAGAGTAG AGAAGCTTGT GCCCAGACCG
    GGTAGTGGTA GCTCGTCAGG ATCAAGTAAC TCTGGATCCC AAGCAGGATC CCACCCAGGA
    TCTCAGAGTG GATCTGGAGA GCGCTTCCGG ATGAGATCAT CTTCAAAATC TGAGGGCTCA
    CCATCTCAAC GACATGAAAA CACAGCCAAA AAGTCTGAAG AGAAGAAAGA AGCAGTTAGA
    CAGGCCAAAG CTCCTGGAGA AGTGGATCTT ACTGCACTCG CTAAGGAGCT TCGAGCAGTA
    GATGATGTTC GGCCACTTCA CAAAGTTACT GACTATTCCT CATCCAGTGA AGAGTCTGGT
    ACTACAGATG AGGAGGATGA TGACATGGAA CAGGAAGGAG CAGATGAATC GACTTCAGGC
    CCAGAGGATG CTAGAGCTAT GTCATCTTTG AATTTAAGCA ATGGAGAAAC GGAGTCTGTG
    AAAACCATGA TCGTACATGA TGATGTCGAA AGTGAACCTG CCATGACCCC ATCTAAAGAA
    GGCACTTTAA TTGTCCGACA GACTCAGTCA GCTAGTAATA CTCTCCAGAA ACACAAATCG
    TCCTCCTCTT TCACACCGTT CATAGATCCA AGGTTACTTC AGATCTCTCC ATCTAGTGGA
    ACAACTGTTA CGTCAGTAGT GGGATTTTCC AGTGAAGCGC TAAGGCCGGA TGCCATGAGG
    CAAGATCCTA CAAGGAAAGG ATCAGTTGTT AATGTTAATC CCACAAATAC TCGGCCACAA
    AGTGATACAC CAGAGATTCG CAAATACAAG AAGAGATTTA ATTCTGAGAT TTTGTGTGCG
    GCATTGTGGG GTGTAAATTT GCTGGTGGGA ACTGAAAGTG GTTTAATGCT GTTGGATAGA
    AGTGGACAAG GGAAAGTGTA CCCCCTCATT AACAGAAGGC GATTTCAGCA GATGGATGTG
    CTTGAAGGCT TGAATGTCTT GGTAACTATA TCAGGGAAAA AGAACAAGCT GAGAGTATAT
    TACTTATCCT GGCTGAGGAA TAAAATTTTG CACAATGATC CTGAAGTAGA GAAGAAACAA
    GGATGGACAA CAGTTGGGGA TTTGGAAGGC TGTGTGCACT ACAAAGTTGT AAAATATGAA
    CGTATCAAGT TTTTGGTAAT CGCTTTAAAA AGTTCAGTGG AGGTGTATGC CTGGGCACCG
    AAACCATACC ACAAGTTTAT GGCCTTTAAG TCATTCGGAG ATTTAGTACA CAGACCTCTC
    CTTGTAGACC TGACTGTGGA AGAAGGACAG AGGCTGAAAG TGATCTATGG TTCCTGTGCT
    GGGTTTCATG CAGTAGATGT GGACTCGGGC GCAGTTTATG ATATTTACTT GCCAACACAT
    ATTCAGAGCA GCATTCAGCC ACACGCAATC ATCATTCTCC CAAATACTGA TGGTATGGAA
    CTGCTGGTTT GCTATGAAGA TGAAGGAGTT TATGTTAATA CATACGGCAG GATCACCAAA
    GATGTAGTTC TACAATGGGG AGAAATGCCT ACATCAGTTG CTTATATCCG ATCTAACCAG
    ATCATGGGAT GGGGTGAAAA AGCTATTGAG ATTCGATCCG TGGAAACTGG CCACCTTGAT
    GGAGTATTTA TGCACAAAAG GGCACAAAGG CTCAAGTTCT TGTGCGAACG AAATGACAAG
    GTTTTCTTTG CCTCGGTGCG GTCTGGCGGA AGCAGCCAAG TGTATTTCAT GACTTTGGGC
    AGAAGCTCTC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813126.1
    CDS344..4087
    Translation

    Target ORF information:

    RefSeq Version XM_012957672.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 4 (map4k4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957672.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCCAACG ATTCGCCTGC CAAAAGTCTT GTGGATATAG ACCTGTCGTC TCTGAGGGAC 
    CCAGCTGGCA TCTTTGAATT AGTGGAAGTG GTAGGAAATG GCACCTATGG ACAAGTATAT
    AAGGGCCGAC ATGTCAAAAC AGGCCAACTT GCAGCCATCA AAGTCATGGA TGTTACAGAG
    GATGAAGAGG AAGAAATCAA ACTGGAAATC AATATGTTGA AGAAATATTC CCACCACAGA
    AATATAGCTA CTTATTATGG TGCTTTTATT AAGAAAAGTC CTCCTGGGCA TGATGACCAG
    CTTTGGTTGG TGATGGAGTT TTGTGGTGCT GGCTCAATCA CTGACCTGGT GAAGAATACA
    AAGGGGAATA GTTTGAAAGA AGACTGGATT GCCTATATCT CTCGTGAGAT ACTTAGGGGA
    TTGGCCCACC TCCATGCTCA TCATGTGATT CACCGGGATA TCAAAGGACA GAATGTGCTG
    CTAACAGAGA ATGCCGAAGT GAAATTAGTG GATTTTGGTG TTAGTGCACA GCTGGATAGG
    ACAGTGGGCC GAAGGAACAC ATTTATTGGT ACACCATACT GGATGGCTCC AGAAGTTATC
    GCCTGTGATG AGAACCCAGA TGCTACCTAT GACTATAGGA GTGACCTCTG GTCATGTGGT
    ATTACAGCGA TTGAAATGGC AGAAGGTGCT CCTCCTCTTT GCGATATGCA TCCAATGCGA
    GCCCTGTTCC TTATCCCAAG AAATCCTCCA CCACGGCTAA AATCCAAGAA ATGGTCAAAG
    AAGTTCTTCA GCTTTATAGA AGGCTGCCTT GTGAAAAATT ACATGCAACG TCCCCCAACA
    GAACAGCTTT TAAAGCATCC ATTTATACGA GATCAGCCAA ATGAAAGGCA AGTCCGAATT
    CAGCTGAAGG ATCACATAGA CAGGACAAGG AAGAAGAGAG GAGAAAAAGA TGAAACAGAG
    TATGAATACA GTGGAAGTGA AGAGGAGGAA GAGGAAGTGC CTGAACAGGA AGGAGAACCA
    AGTTCCATTG TTAATGTGCC TGGGGAATCA ACACTCCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGCTC TGAGGCTTTG CGACGGCAAC AGCTGCTGCA GGAGCAACAA
    CTCAGAGAGC AGGAGGAATA TAAAAGACAG CTCCTGGCAG AGAGACAGAA GCGCATAGAA
    CAGCAGAAAG AGCAAAGGAG GAGACTTGAA GAGCAACAAA GAAGAGAGCG TGAGGCTCGT
    CGGCAGCAGG AACGAGAACA AAGAAGGCGA GAGCAAGAGG AAAAGAAGAG AATGGAGGAG
    ATGGAAAGAA GGCGCAAGGA AGAGGAAGAA AGGAGAAGAG CAGAGGAAGA GAAAAGAAGA
    GTGGAAAGAG AGCAGGAGTA TATCCGAAGA CAGCTGGAGG AGGAGCAGCG GCACTTAGAA
    ATCCTTCAGC AGCAGCTGCT TCAGGAGCAG GCCATGTTAC TGGAGTATAG ATGGCGTGAA
    ATGGAGGAGC ACCGCCAGGC TGAAAGACTT CAGCGTCAGT TGCAGCAAGA GCAGGCCTAT
    CTCATGTCAC TACAGCATGA TCATAGACAG CAGCAGCCAC CACAACAGCA GCCCCAGCCC
    CAGCAGCCTC CACTGTCCCA GCAGGAAAGG AGCAAGCAAA CATACCATAC CCAAGAACCC
    AAGCCTCATT ATGAACAAGC AGAACGAATA CGAGAGGCAG AGGAGCGCCT TAGGAAAGCA
    AACCAAGGCT CCCCTGAAGC CCAGGCTAAG CAACCAGGAA GAGTTTTAGA GCCATTAGTG
    CCTTCAAGAT CCGAATCATT TTCCAATGGA AATTCTGAGC CTGCTCAACC TGCCATGCAC
    AGACCAGTGG AACCACAGGT GCCTGTGAGG ACAACATCAA GATCTCCAGT ATTGTCACGG
    AAAGATTCAC CACTGCAGGG CAGTGGGCAG CAAAATACCC AAGCTGGTCA GAGAAATTCC
    ACAAGCAATA TTGAGCCTAG ACTTCTTTGG GAAAGAGTAG AGAAGCTTGT GCCCAGACCG
    GGTAGTGGTA GCTCGTCAGG ATCAAGTAAC TCTGGATCCC AAGCAGGATC CCACCCAGGA
    TCTCAGAGTG GATCTGGAGA GCGCTTCCGG ATGAGATCAT CTTCAAAATC TGAGGGCTCA
    CCATCTCAAC GACATGAAAA CACAGCCAAA AAGTCTGAAG AGAAGAAAGA AGCAGTTAGA
    CAGGCCAAAG CTCCTGGAGA AGTGGATCTT ACTGCACTCG CTAAGGAGCT TCGAGCAGTA
    GATGATGTTC GGCCACTTCA CAAAGTTACT GACTATTCCT CATCCAGTGA AGAGTCTGGT
    ACTACAGATG AGGAGGATGA TGACATGGAA CAGGAAGGAG CAGATGAATC GACTTCAGGC
    CCAGAGGATG CTAGAGCTAT GTCATCTTTG AATTTAAGCA ATGGAGAAAC GGAGTCTGTG
    AAAACCATGA TCGTACATGA TGATGTCGAA AGTGAACCTG CCATGACCCC ATCTAAAGAA
    GGCACTTTAA TTGTCCGACA GACTCAGTCA GCTAGTAATA CTCTCCAGAA ACACAAATCG
    TCCTCCTCTT TCACACCGTT CATAGATCCA AGGTTACTTC AGATCTCTCC ATCTAGTGGA
    ACAACTGTTA CGTCAGTAGT GGGATTTTCC AGTGAAGCGC TAAGGCCGGA TGCCATGAGG
    CAAGATCCTA CAAGGAAAGG ATCAGTTGTT AATGTTAATC CCACAAATAC TCGGCCACAA
    AGTGATACAC CAGAGATTCG CAAATACAAG AAGAGATTTA ATTCTGAGAT TTTGTGTGCG
    GCATTGTGGG GTGTAAATTT GCTGGTGGGA ACTGAAAGTG GTTTAATGCT GTTGGATAGA
    AGTGGACAAG GGAAAGTGTA CCCCCTCATT AACAGAAGGC GATTTCAGCA GATGGATGTG
    CTTGAAGGCT TGAATGTCTT GGTAACTATA TCAGGGAAAA AGAACAAGCT GAGAGTATAT
    TACTTATCCT GGCTGAGGAA TAAAATTTTG CACAATGATC CTGAAGTAGA GAAGAAACAA
    GGATGGACAA CAGTTGGGGA TTTGGAAGGC TGTGTGCACT ACAAAGTTGT AAAATATGAA
    CGTATCAAGT TTTTGGTAAT CGCTTTAAAA AGTTCAGTGG AGGTGTATGC CTGGGCACCG
    AAACCATACC ACAAGTTTAT GGCCTTTAAG TCATTCGGAG ATTTAGTACA CAGACCTCTC
    CTTGTAGACC TGACTGTGGA AGAAGGACAG AGGCTGAAAG TGATCTATGG TTCCTGTGCT
    GGGTTTCATG CAGTAGATGT GGACTCGGGC GCAGTTTATG ATATTTACTT GCCAACACAT
    ATTCAGAGCA GCATTCAGCC ACACGCAATC ATCATTCTCC CAAATACTGA TGGTATGGAA
    CTGCTGGTTT GCTATGAAGA TGAAGGAGTT TATGTTAATA CATACGGCAG GATCACCAAA
    GATGTAGTTC TACAATGGGG AGAAATGCCT ACATCAGTTG CTTATATCCG ATCTAACCAG
    ATCATGGGAT GGGGTGAAAA AGCTATTGAG ATTCGATCCG TGGAAACTGG CCACCTTGAT
    GGAGTATTTA TGCACAAAAG GGCACAAAGG CTCAAGTTCT TGTGCGAACG AAATGACAAG
    GTTTTCTTTG CCTCGGTGCG GTCTGGCGGA AGCAGCCAAG TGTATTTCAT GACTTTGGGC
    AGAAGCTCTC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35141
    Clone ID Related Accession (Same CDS sequence) XM_012957673.2 , XM_012957673.3
    Accession Version XM_012957673.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3927bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957673.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCCAACG ATTCGCCTGC CAAAAGTCTT GTGGATATAG ACCTGTCGTC TCTGAGGGAC 
    CCAGCTGGCA TCTTTGAATT AGTGGAAGTG GTAGGAAATG GCACCTATGG ACAAGTATAT
    AAGGGCCGAC ATGTCAAAAC AGGCCAACTT GCAGCCATCA AAGTCATGGA TGTTACAGAG
    GATGAAGAGG AAGAAATCAA ACTGGAAATC AATATGTTGA AGAAATATTC CCACCACAGA
    AATATAGCTA CTTATTATGG TGCTTTTATT AAGAAAAGTC CTCCTGGGCA TGATGACCAG
    CTTTGGTTGG TGATGGAGTT TTGTGGTGCT GGCTCAATCA CTGACCTGGT GAAGAATACA
    AAGGGGAATA GTTTGAAAGA AGACTGGATT GCCTATATCT CTCGTGAGAT ACTTAGGGGA
    TTGGCCCACC TCCATGCTCA TCATGTGATT CACCGGGATA TCAAAGGACA GAATGTGCTG
    CTAACAGAGA ATGCCGAAGT GAAATTAGTG GATTTTGGTG TTAGTGCACA GCTGGATAGG
    ACAGTGGGCC GAAGGAACAC ATTTATTGGT ACACCATACT GGATGGCTCC AGAAGTTATC
    GCCTGTGATG AGAACCCAGA TGCTACCTAT GACTATAGGA GTGACCTCTG GTCATGTGGT
    ATTACAGCGA TTGAAATGGC AGAAGGTGCT CCTCCTCTTT GCGATATGCA TCCAATGCGA
    GCCCTGTTCC TTATCCCAAG AAATCCTCCA CCACGGCTAA AATCCAAGAA ATGGTCAAAG
    AAGTTCTTCA GCTTTATAGA AGGCTGCCTT GTGAAAAATT ACATGCAACG TCCCCCAACA
    GAACAGCTTT TAAAGCATCC ATTTATACGA GATCAGCCAA ATGAAAGGCA AGTCCGAATT
    CAGCTGAAGG ATCACATAGA CAGGACAAGG AAGAAGAGAG GAGAAAAAGA TGAAACAGAG
    TATGAATACA GTGGAAGTGA AGAGGAGGAA GAGGAAGTGC CTGAACAGGA AGGAGAACCA
    AGTTCCATTG TTAATGTGCC TGGGGAATCA ACACTCCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGCTC TGAGGCTTTG CGACGGCAAC AGCTGCTGCA GGAGCAACAA
    CTCAGAGAGC AGGAGGAATA TAAAAGACAG CTCCTGGCAG AGAGACAGAA GCGCATAGAA
    CAGCAGAAAG AGCAAAGGAG GAGACTTGAA GAGCAACAAA GAAGAGAGCG TGAGGCTCGT
    CGGCAGCAGG AACGAGAACA AAGAAGGCGA GAGCAAGAGG AAAAGAAGAG AATGGAGGAG
    ATGGAAAGAA GGCGCAAGGA AGAGGAAGAA AGGAGAAGAG CAGAGGAAGA GAAAAGAAGA
    GTGGAAAGAG AGCAGGAGTA TATCCGAAGA CAGCTGGAGG AGGAGCAGCG GCACTTAGAA
    ATCCTTCAGC AGCAGCTGCT TCAGGAGCAG GCCATGTTAC TGGAGTATAG ATGGCGTGAA
    ATGGAGGAGC ACCGCCAGGC TGAAAGACTT CAGCGTCAGT TGCAGCAAGA GCAGGCCTAT
    CTCATGTCAC TACAGCATGA TCATAGACAG CAGCAGCCAC CACAACAGCA GCCCCAGCCC
    CAGCAGCCTC CACTGTCCCA GCAGGAAAGG AGCAAGCAAA CATACCATAC CCAAGAACCC
    AAGCCTCATT ATGAACAAGC AGAACGAATA CGAGAGGCAG AGGAGCGCCT TAGGAAAGCA
    AACCAAGGCT CCCCTGAAGC CCAGGCTAAG CAACCAGGAA GAGTTTTAGA GCCATTAGTG
    CCTTCAAGAT CCGAATCATT TTCCAATGGA AATTCTGAGC CTGCTCAACC TGCCATGCAC
    AGACCAGTGG AACCACAGGT GCCTGTGAGG ACAACATCAA GATCTCCAGT ATTGTCACGG
    AAAGATTCAC CACTGCAGGG CAGTGGGCAG CAAAATACCC AAGCTGGTCA GAGAAATTCC
    ACAAGCAATA TTGAGCCTAG ACTTCTTTGG GAAAGAGTAG AGAAGCTTGT GCCCAGACCG
    GGTAGTGGTA GCTCGTCAGG ATCAAGTAAC TCTGGATCCC AAGCAGGATC CCACCCAGGA
    TCTCAGAGTG GATCTGGAGA GCGCTTCCGG ATGAGATCAT CTTCAAAATC TGAGGGCTCA
    CCATCTCAAC GACATGAAAA CACAGCCAAA AAGTCTGAAG AGAAGAAAGA AGCAGTTAGA
    CAGGCCAAAG CTCCTGGAGA AGTGGATCTT ACTGCACTCG CTAAGGAGCT TCGAGCAGTA
    GATGATGTTC GGCCACTTCA CAAAGTTACT GACTATTCCT CATCCAGTGA AGAGTCTGGT
    ACTACAGATG AGGAGGATGA TGACATGGAA CAGGAAGGAG CAGATGAATC GACTTCAGGC
    CCAGAGGATG CTAGAGCTAT GTCATCTTTG AATTTAAGCA ATGGAGAAAC GGAGTCTGTG
    AAAACCATGA TCGTACATGA TGATGTCGAA AGTGAACCTG CCATGACCCC ATCTAAAGAA
    GGCACTTTAA TTGTCCGACA GAGTACAGTT GACAAAAAGC GTGCCAACCA ACTTGAGAGC
    AATGGCTTTG CGAGTCGTAT TCACCTCTTG CCAGATCTCT TACAGCAAAG CCATTCCTCC
    TCCACCACTT CTTCCCCAAC CTCAAGTCAA CCGACACCTA CCATGTCCCC CCAGACACCC
    CAGGACAAGC TAACTGCTAA TGAGACTCAG TCAGCTAGTA ATACTCTCCA GAAACACAAA
    TCGTCCTCCT CTTTCACACC GTTCATAGAT CCAAGGTTAC TTCAGATCTC TCCATCTAGT
    GGAACAACTG TTACGTCAGT AGTGGGATTT TCCAGTGAAG CGCTAAGGCC GGATGCCATG
    AGGCAAGATC CTACAAGGAA AGGATCAGTT GTTAATGTTA ATCCCACAAA TACTCGGCCA
    CAAAGTGATA CACCAGAGAT TCGCAAATAC AAGAAGAGAT TTAATTCTGA GATTTTGTGT
    GCGGCATTGT GGGGTGTAAA TTTGCTGGTG GGAACTGAAA GTGGTTTAAT GCTGTTGGAT
    AGAAGTGGAC AAGGGAAAGT GTACCCCCTC ATTAACAGAA GGCGATTTCA GCAGATGGAT
    GTGCTTGAAG GCTTGAATGT CTTGGTAACT ATATCAGGGA AAAAGAACAA GCTGAGAGTA
    TATTACTTAT CCTGGCTGAG GAATAAAATT TTGCACAATG ATCCTGAAGT AGAGAAGAAA
    CAAGGATGGA CAACAGTTGG GGATTTGGAA GGCTGTGTGC ACTACAAAGT TGTAAAATAT
    GAACGTATCA AGTTTTTGGT AATCGCTTTA AAAAGTTCAG TGGAGGTGTA TGCCTGGGCA
    CCGAAACCAT ACCACAAGTT TATGGCCTTT AAGTCATTCG GAGATTTAGT ACACAGACCT
    CTCCTTGTAG ACCTGACTGT GGAAGAAGGA CAGAGGCTGA AAGTGATCTA TGGTTCCTGT
    GCTGGGTTTC ATGCAGTAGA TGTGGACTCG GGCGCAGTTT ATGATATTTA CTTGCCAACA
    CATATTCAGA GCAGCATTCA GCCACACGCA ATCATCATTC TCCCAAATAC TGATGGTATG
    GAACTGCTGG TTTGCTATGA AGATGAAGGA GTTTATGTTA ATACATACGG CAGGATCACC
    AAAGATGTAG TTCTACAATG GGGAGAAATG CCTACATCAG TTGCTTATAT CCGATCTAAC
    CAGATCATGG GATGGGGTGA AAAAGCTATT GAGATTCGAT CCGTGGAAAC TGGCCACCTT
    GATGGAGTAT TTATGCACAA AAGGGCACAA AGGCTCAAGT TCTTGTGCGA ACGAAATGAC
    AAGGTTTTCT TTGCCTCGGT GCGGTCTGGC GGAAGCAGCC AAGTGTATTT CATGACTTTG
    GGCAGAAGCT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813127.1
    CDS344..4270
    Translation

    Target ORF information:

    RefSeq Version XM_012957673.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 4 (map4k4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957673.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCCAACG ATTCGCCTGC CAAAAGTCTT GTGGATATAG ACCTGTCGTC TCTGAGGGAC 
    CCAGCTGGCA TCTTTGAATT AGTGGAAGTG GTAGGAAATG GCACCTATGG ACAAGTATAT
    AAGGGCCGAC ATGTCAAAAC AGGCCAACTT GCAGCCATCA AAGTCATGGA TGTTACAGAG
    GATGAAGAGG AAGAAATCAA ACTGGAAATC AATATGTTGA AGAAATATTC CCACCACAGA
    AATATAGCTA CTTATTATGG TGCTTTTATT AAGAAAAGTC CTCCTGGGCA TGATGACCAG
    CTTTGGTTGG TGATGGAGTT TTGTGGTGCT GGCTCAATCA CTGACCTGGT GAAGAATACA
    AAGGGGAATA GTTTGAAAGA AGACTGGATT GCCTATATCT CTCGTGAGAT ACTTAGGGGA
    TTGGCCCACC TCCATGCTCA TCATGTGATT CACCGGGATA TCAAAGGACA GAATGTGCTG
    CTAACAGAGA ATGCCGAAGT GAAATTAGTG GATTTTGGTG TTAGTGCACA GCTGGATAGG
    ACAGTGGGCC GAAGGAACAC ATTTATTGGT ACACCATACT GGATGGCTCC AGAAGTTATC
    GCCTGTGATG AGAACCCAGA TGCTACCTAT GACTATAGGA GTGACCTCTG GTCATGTGGT
    ATTACAGCGA TTGAAATGGC AGAAGGTGCT CCTCCTCTTT GCGATATGCA TCCAATGCGA
    GCCCTGTTCC TTATCCCAAG AAATCCTCCA CCACGGCTAA AATCCAAGAA ATGGTCAAAG
    AAGTTCTTCA GCTTTATAGA AGGCTGCCTT GTGAAAAATT ACATGCAACG TCCCCCAACA
    GAACAGCTTT TAAAGCATCC ATTTATACGA GATCAGCCAA ATGAAAGGCA AGTCCGAATT
    CAGCTGAAGG ATCACATAGA CAGGACAAGG AAGAAGAGAG GAGAAAAAGA TGAAACAGAG
    TATGAATACA GTGGAAGTGA AGAGGAGGAA GAGGAAGTGC CTGAACAGGA AGGAGAACCA
    AGTTCCATTG TTAATGTGCC TGGGGAATCA ACACTCCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGCTC TGAGGCTTTG CGACGGCAAC AGCTGCTGCA GGAGCAACAA
    CTCAGAGAGC AGGAGGAATA TAAAAGACAG CTCCTGGCAG AGAGACAGAA GCGCATAGAA
    CAGCAGAAAG AGCAAAGGAG GAGACTTGAA GAGCAACAAA GAAGAGAGCG TGAGGCTCGT
    CGGCAGCAGG AACGAGAACA AAGAAGGCGA GAGCAAGAGG AAAAGAAGAG AATGGAGGAG
    ATGGAAAGAA GGCGCAAGGA AGAGGAAGAA AGGAGAAGAG CAGAGGAAGA GAAAAGAAGA
    GTGGAAAGAG AGCAGGAGTA TATCCGAAGA CAGCTGGAGG AGGAGCAGCG GCACTTAGAA
    ATCCTTCAGC AGCAGCTGCT TCAGGAGCAG GCCATGTTAC TGGAGTATAG ATGGCGTGAA
    ATGGAGGAGC ACCGCCAGGC TGAAAGACTT CAGCGTCAGT TGCAGCAAGA GCAGGCCTAT
    CTCATGTCAC TACAGCATGA TCATAGACAG CAGCAGCCAC CACAACAGCA GCCCCAGCCC
    CAGCAGCCTC CACTGTCCCA GCAGGAAAGG AGCAAGCAAA CATACCATAC CCAAGAACCC
    AAGCCTCATT ATGAACAAGC AGAACGAATA CGAGAGGCAG AGGAGCGCCT TAGGAAAGCA
    AACCAAGGCT CCCCTGAAGC CCAGGCTAAG CAACCAGGAA GAGTTTTAGA GCCATTAGTG
    CCTTCAAGAT CCGAATCATT TTCCAATGGA AATTCTGAGC CTGCTCAACC TGCCATGCAC
    AGACCAGTGG AACCACAGGT GCCTGTGAGG ACAACATCAA GATCTCCAGT ATTGTCACGG
    AAAGATTCAC CACTGCAGGG CAGTGGGCAG CAAAATACCC AAGCTGGTCA GAGAAATTCC
    ACAAGCAATA TTGAGCCTAG ACTTCTTTGG GAAAGAGTAG AGAAGCTTGT GCCCAGACCG
    GGTAGTGGTA GCTCGTCAGG ATCAAGTAAC TCTGGATCCC AAGCAGGATC CCACCCAGGA
    TCTCAGAGTG GATCTGGAGA GCGCTTCCGG ATGAGATCAT CTTCAAAATC TGAGGGCTCA
    CCATCTCAAC GACATGAAAA CACAGCCAAA AAGTCTGAAG AGAAGAAAGA AGCAGTTAGA
    CAGGCCAAAG CTCCTGGAGA AGTGGATCTT ACTGCACTCG CTAAGGAGCT TCGAGCAGTA
    GATGATGTTC GGCCACTTCA CAAAGTTACT GACTATTCCT CATCCAGTGA AGAGTCTGGT
    ACTACAGATG AGGAGGATGA TGACATGGAA CAGGAAGGAG CAGATGAATC GACTTCAGGC
    CCAGAGGATG CTAGAGCTAT GTCATCTTTG AATTTAAGCA ATGGAGAAAC GGAGTCTGTG
    AAAACCATGA TCGTACATGA TGATGTCGAA AGTGAACCTG CCATGACCCC ATCTAAAGAA
    GGCACTTTAA TTGTCCGACA GAGTACAGTT GACAAAAAGC GTGCCAACCA ACTTGAGAGC
    AATGGCTTTG CGAGTCGTAT TCACCTCTTG CCAGATCTCT TACAGCAAAG CCATTCCTCC
    TCCACCACTT CTTCCCCAAC CTCAAGTCAA CCGACACCTA CCATGTCCCC CCAGACACCC
    CAGGACAAGC TAACTGCTAA TGAGACTCAG TCAGCTAGTA ATACTCTCCA GAAACACAAA
    TCGTCCTCCT CTTTCACACC GTTCATAGAT CCAAGGTTAC TTCAGATCTC TCCATCTAGT
    GGAACAACTG TTACGTCAGT AGTGGGATTT TCCAGTGAAG CGCTAAGGCC GGATGCCATG
    AGGCAAGATC CTACAAGGAA AGGATCAGTT GTTAATGTTA ATCCCACAAA TACTCGGCCA
    CAAAGTGATA CACCAGAGAT TCGCAAATAC AAGAAGAGAT TTAATTCTGA GATTTTGTGT
    GCGGCATTGT GGGGTGTAAA TTTGCTGGTG GGAACTGAAA GTGGTTTAAT GCTGTTGGAT
    AGAAGTGGAC AAGGGAAAGT GTACCCCCTC ATTAACAGAA GGCGATTTCA GCAGATGGAT
    GTGCTTGAAG GCTTGAATGT CTTGGTAACT ATATCAGGGA AAAAGAACAA GCTGAGAGTA
    TATTACTTAT CCTGGCTGAG GAATAAAATT TTGCACAATG ATCCTGAAGT AGAGAAGAAA
    CAAGGATGGA CAACAGTTGG GGATTTGGAA GGCTGTGTGC ACTACAAAGT TGTAAAATAT
    GAACGTATCA AGTTTTTGGT AATCGCTTTA AAAAGTTCAG TGGAGGTGTA TGCCTGGGCA
    CCGAAACCAT ACCACAAGTT TATGGCCTTT AAGTCATTCG GAGATTTAGT ACACAGACCT
    CTCCTTGTAG ACCTGACTGT GGAAGAAGGA CAGAGGCTGA AAGTGATCTA TGGTTCCTGT
    GCTGGGTTTC ATGCAGTAGA TGTGGACTCG GGCGCAGTTT ATGATATTTA CTTGCCAACA
    CATATTCAGA GCAGCATTCA GCCACACGCA ATCATCATTC TCCCAAATAC TGATGGTATG
    GAACTGCTGG TTTGCTATGA AGATGAAGGA GTTTATGTTA ATACATACGG CAGGATCACC
    AAAGATGTAG TTCTACAATG GGGAGAAATG CCTACATCAG TTGCTTATAT CCGATCTAAC
    CAGATCATGG GATGGGGTGA AAAAGCTATT GAGATTCGAT CCGTGGAAAC TGGCCACCTT
    GATGGAGTAT TTATGCACAA AAGGGCACAA AGGCTCAAGT TCTTGTGCGA ACGAAATGAC
    AAGGTTTTCT TTGCCTCGGT GCGGTCTGGC GGAAGCAGCC AAGTGTATTT CATGACTTTG
    GGCAGAAGCT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42793
    Clone ID Related Accession (Same CDS sequence) XM_018091631.1
    Accession Version XM_018091631.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4206bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCCAACG ATTCGCCTGC CAAAAGTCTT GTGGATATAG ACCTGTCGTC TCTGAGGGAC 
    CCAGCTGGCA TCTTTGAATT AGTGGAAGTG GTAGGAAATG GCACCTATGG ACAAGTATAT
    AAGGGCCGAC ATGTCAAAAC AGGCCAACTT GCAGCCATCA AAGTCATGGA TGTTACAGAG
    GATGAAGAGG AAGAAATCAA ACTGGAAATC AATATGTTGA AGAAATATTC CCACCACAGA
    AATATAGCTA CTTATTATGG TGCTTTTATT AAGAAAAGTC CTCCTGGGCA TGATGACCAG
    CTTTGGTTGG TGATGGAGTT TTGTGGTGCT GGCTCAATCA CTGACCTGGT GAAGAATACA
    AAGGGGAATA GTTTGAAAGA AGACTGGATT GCCTATATCT CTCGTGAGAT ACTTAGGGGA
    TTGGCCCACC TCCATGCTCA TCATGTGATT CACCGGGATA TCAAAGGACA GAATGTGCTG
    CTAACAGAGA ATGCCGAAGT GAAATTAGTG GATTTTGGTG TTAGTGCACA GCTGGATAGG
    ACAGTGGGCC GAAGGAACAC ATTTATTGGT ACACCATACT GGATGGCTCC AGAAGTTATC
    GCCTGTGATG AGAACCCAGA TGCTACCTAT GACTATAGGA GTGACCTCTG GTCATGTGGT
    ATTACAGCGA TTGAAATGGC AGAAGGTGCT CCTCCTCTTT GCGATATGCA TCCAATGCGA
    GCCCTGTTCC TTATCCCAAG AAATCCTCCA CCACGGCTAA AATCCAAGAA ATGGTCAAAG
    AAGTTCTTCA GCTTTATAGA AGGCTGCCTT GTGAAAAATT ACATGCAACG TCCCCCAACA
    GAACAGCTTT TAAAGCATCC ATTTATACGA GATCAGCCAA ATGAAAGGCA AGTCCGAATT
    CAGCTGAAGG ATCACATAGA CAGGACAAGG AAGAAGAGAG GAGAAAAAGA TGAAACAGAG
    TATGAATACA GTGGAAGTGA AGAGGAGGAA GAGGAAGTGC CTGAACAGGA AGGAGAACCA
    AGTTCCATTG TTAATGTGCC TGGGGAATCA ACACTCCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGCTC TGAGGCTTTG CGACGGCAAC AGCTGCTGCA GGAGCAACAA
    CTCAGAGAGC AGGAGGAATA TAAAAGACAG CTCCTGGCAG AGAGACAGAA GCGCATAGAA
    CAGCAGAAAG AGCAAAGGAG GAGACTTGAA GAGCAACAAA GAAGAGAGCG TGAGGCTCGT
    CGGCAGCAGG AACGAGAACA AAGAAGGCGA GAGCAAGAGG AAAAGAAGAG AATGGAGGAG
    ATGGAAAGAA GGCGCAAGGA AGAGGAAGAA AGGAGAAGAG CAGAGGAAGA GAAAAGAAGA
    GTGGAAAGAG AGCAGGAGTA TATCCGAAGA CAGCTGGAGG AGGAGCAGCG GCACTTAGAA
    ATCCTTCAGC AGCAGCTGCT TCAGGAGCAG GCCATGTTAC TGGAGTATAG ATGGCGTGAA
    ATGGAGGAGC ACCGCCAGGC TGAAAGACTT CAGCGTCAGT TGCAGCAAGA GCAGGCCTAT
    CTCATGTCAC TACAGCATGA TCATAGACAG CAGCAGCCAC CACAACAGCA GCCCCAGCCC
    CAGCAGCCTC CACTGTCCCA GCAGGAAAGG AGCAAGCAAA CATACCATAC CCAAGAACCC
    AAGCCTCATT ATGAACAAGC AGAACGAATA CGAGAGGCAG AGGAGCGCCT TAGGAAAGCA
    AACCAAGGCT CCCCTGAAGC CCAGGCTAAG CAACCAGGAA GAGTTTTAGA GCCATTAGTG
    CCTTCAAGAT CCGAATCATT TTCCAATGGA AATTCTGAGC CTGCTCAACC TGCCATGCAC
    AGACCAGTGG AACCACAGTG GTCTCATCTG GCATCCCTCA AGAACAATGT ATCCCCTGTC
    TCGCGATCCC ATTCCTTCAG TGACCCTTCT CCCAAATTTG CACCTCACCT TCTACGATCT
    CAGGATTCGC ACCAAACTTC ACGCAGTGAA GTGCAAAGTC AGGGTTCTGA CCCTAAGTCT
    GAAATCTCTG AGCCAACCCA AAGGGTTTGG GCTCGATCAG ACAGTGACGA GGTGCCTCCA
    AGGGTGCCTG TGAGGACAAC ATCAAGATCT CCAGTATTGT CACGGAAAGA TTCACCACTG
    CAGGGCAGTG GGCAGCAAAA TACCCAAGCT GGTCAGAGAA ATTCCACAAG CAATATTGAG
    CCTAGACTTC TTTGGGAAAG AGTAGAGAAG CTTGTGCCCA GACCGGGTAG TGGTAGCTCG
    TCAGGATCAA GTAACTCTGG ATCCCAAGCA GGATCCCACC CAGGATCTCA GAGTGGATCT
    GGAGAGCGCT TCCGGATGAG ATCATCTTCA AAATCTGAGG GCTCACCATC TCAACGACAT
    GAAAACACAG CCAAAAAGTC TGAAGAGAAG AAAGAAGCAG TTAGACAGGC CAAAGCTCCT
    GGAGAAGTGG ATCTTACTGC ACTCGCTAAG GAGCTTCGAG CAGTAGATGA TGTTCGGCCA
    CTTCACAAAG TTACTGACTA TTCCTCATCC AGTGAAGAGT CTGGTACTAC AGATGAGGAG
    GATGATGACA TGGAACAGGA AGGAGCAGAT GAATCGACTT CAGGCCCAGA GGATGCTAGA
    GCTATGTCAT CTTTGAATTT AAGCAATGGA GAAACGGAGT CTGTGAAAAC CATGATCGTA
    CATGATGATG TCGAAAGTGA ACCTGCCATG ACCCCATCTA AAGAAGGCAC TTTAATTGTC
    CGACAGAGTA CAGTTGACAA AAAGCGTGCC AACCAACTTG AGAGCAATGG CTTTGCGAGT
    CGTATTCACC TCTTGCCAGA TCTCTTACAG CAAAGCCATT CCTCCTCCAC CACTTCTTCC
    CCAACCTCAA GTCAACCGAC ACCTACCATG TCCCCCCAGA CACCCCAGGA CAAGCTAACT
    GCTAATGAGA CTCAGTCAGC TAGTAATACT CTCCAGAAAC ACAAATCGTC CTCCTCTTTC
    ACACCGTTCA TAGATCCAAG GTTACTTCAG ATCTCTCCAT CTAGTGGAAC AACTGTTACG
    TCAGTAGGTA CATACATGCG CACACTGGAA GCTAACCTTA AAAATACTAA TTTTATTTTA
    GTGGGATTTT CCAGTGAAGC GCTAAGGCCG GATGCCATGA GGCAAGATCC TACAAGGAAA
    GGATCAGTTG TTAATGTTAA TCCCACAAAT ACTCGGCCAC AAAGTGATAC ACCAGAGATT
    CGCAAATACA AGAAGAGATT TAATTCTGAG ATTTTGTGTG CGGCATTGTG GGGTGTAAAT
    TTGCTGGTGG GAACTGAAAG TGGTTTAATG CTGTTGGATA GAAGTGGACA AGGGAAAGTG
    TACCCCCTCA TTAACAGAAG GCGATTTCAG CAGATGGATG TGCTTGAAGG CTTGAATGTC
    TTGGTAACTA TATCAGGGAA AAAGAACAAG CTGAGAGTAT ATTACTTATC CTGGCTGAGG
    AATAAAATTT TGCACAATGA TCCTGAAGTA GAGAAGAAAC AAGGATGGAC AACAGTTGGG
    GATTTGGAAG GCTGTGTGCA CTACAAAGTT GTAAAATATG AACGTATCAA GTTTTTGGTA
    ATCGCTTTAA AAAGTTCAGT GGAGGTGTAT GCCTGGGCAC CGAAACCATA CCACAAGTTT
    ATGGCCTTTA AGTCATTCGG AGATTTAGTA CACAGACCTC TCCTTGTAGA CCTGACTGTG
    GAAGAAGGAC AGAGGCTGAA AGTGATCTAT GGTTCCTGTG CTGGGTTTCA TGCAGTAGAT
    GTGGACTCGG GCGCAGTTTA TGATATTTAC TTGCCAACAC ATATTCAGAG CAGCATTCAG
    CCACACGCAA TCATCATTCT CCCAAATACT GATGGTATGG AACTGCTGGT TTGCTATGAA
    GATGAAGGAG TTTATGTTAA TACATACGGC AGGATCACCA AAGATGTAGT TCTACAATGG
    GGAGAAATGC CTACATCAGT TGCTTATATC CGATCTAACC AGATCATGGG ATGGGGTGAA
    AAAGCTATTG AGATTCGATC CGTGGAAACT GGCCACCTTG ATGGAGTATT TATGCACAAA
    AGGGCACAAA GGCTCAAGTT CTTGTGCGAA CGAAATGACA AGGTTTTCTT TGCCTCGGTG
    CGGTCTGGCG GAAGCAGCCA AGTGTATTTC ATGACTTTGG GCAGAAGCTC TCTTCTGAGC
    TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947120.1
    CDS348..4553
    Translation

    Target ORF information:

    RefSeq Version XM_018091631.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 4 (map4k4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091631.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCCAACG ATTCGCCTGC CAAAAGTCTT GTGGATATAG ACCTGTCGTC TCTGAGGGAC 
    CCAGCTGGCA TCTTTGAATT AGTGGAAGTG GTAGGAAATG GCACCTATGG ACAAGTATAT
    AAGGGCCGAC ATGTCAAAAC AGGCCAACTT GCAGCCATCA AAGTCATGGA TGTTACAGAG
    GATGAAGAGG AAGAAATCAA ACTGGAAATC AATATGTTGA AGAAATATTC CCACCACAGA
    AATATAGCTA CTTATTATGG TGCTTTTATT AAGAAAAGTC CTCCTGGGCA TGATGACCAG
    CTTTGGTTGG TGATGGAGTT TTGTGGTGCT GGCTCAATCA CTGACCTGGT GAAGAATACA
    AAGGGGAATA GTTTGAAAGA AGACTGGATT GCCTATATCT CTCGTGAGAT ACTTAGGGGA
    TTGGCCCACC TCCATGCTCA TCATGTGATT CACCGGGATA TCAAAGGACA GAATGTGCTG
    CTAACAGAGA ATGCCGAAGT GAAATTAGTG GATTTTGGTG TTAGTGCACA GCTGGATAGG
    ACAGTGGGCC GAAGGAACAC ATTTATTGGT ACACCATACT GGATGGCTCC AGAAGTTATC
    GCCTGTGATG AGAACCCAGA TGCTACCTAT GACTATAGGA GTGACCTCTG GTCATGTGGT
    ATTACAGCGA TTGAAATGGC AGAAGGTGCT CCTCCTCTTT GCGATATGCA TCCAATGCGA
    GCCCTGTTCC TTATCCCAAG AAATCCTCCA CCACGGCTAA AATCCAAGAA ATGGTCAAAG
    AAGTTCTTCA GCTTTATAGA AGGCTGCCTT GTGAAAAATT ACATGCAACG TCCCCCAACA
    GAACAGCTTT TAAAGCATCC ATTTATACGA GATCAGCCAA ATGAAAGGCA AGTCCGAATT
    CAGCTGAAGG ATCACATAGA CAGGACAAGG AAGAAGAGAG GAGAAAAAGA TGAAACAGAG
    TATGAATACA GTGGAAGTGA AGAGGAGGAA GAGGAAGTGC CTGAACAGGA AGGAGAACCA
    AGTTCCATTG TTAATGTGCC TGGGGAATCA ACACTCCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGCTC TGAGGCTTTG CGACGGCAAC AGCTGCTGCA GGAGCAACAA
    CTCAGAGAGC AGGAGGAATA TAAAAGACAG CTCCTGGCAG AGAGACAGAA GCGCATAGAA
    CAGCAGAAAG AGCAAAGGAG GAGACTTGAA GAGCAACAAA GAAGAGAGCG TGAGGCTCGT
    CGGCAGCAGG AACGAGAACA AAGAAGGCGA GAGCAAGAGG AAAAGAAGAG AATGGAGGAG
    ATGGAAAGAA GGCGCAAGGA AGAGGAAGAA AGGAGAAGAG CAGAGGAAGA GAAAAGAAGA
    GTGGAAAGAG AGCAGGAGTA TATCCGAAGA CAGCTGGAGG AGGAGCAGCG GCACTTAGAA
    ATCCTTCAGC AGCAGCTGCT TCAGGAGCAG GCCATGTTAC TGGAGTATAG ATGGCGTGAA
    ATGGAGGAGC ACCGCCAGGC TGAAAGACTT CAGCGTCAGT TGCAGCAAGA GCAGGCCTAT
    CTCATGTCAC TACAGCATGA TCATAGACAG CAGCAGCCAC CACAACAGCA GCCCCAGCCC
    CAGCAGCCTC CACTGTCCCA GCAGGAAAGG AGCAAGCAAA CATACCATAC CCAAGAACCC
    AAGCCTCATT ATGAACAAGC AGAACGAATA CGAGAGGCAG AGGAGCGCCT TAGGAAAGCA
    AACCAAGGCT CCCCTGAAGC CCAGGCTAAG CAACCAGGAA GAGTTTTAGA GCCATTAGTG
    CCTTCAAGAT CCGAATCATT TTCCAATGGA AATTCTGAGC CTGCTCAACC TGCCATGCAC
    AGACCAGTGG AACCACAGTG GTCTCATCTG GCATCCCTCA AGAACAATGT ATCCCCTGTC
    TCGCGATCCC ATTCCTTCAG TGACCCTTCT CCCAAATTTG CACCTCACCT TCTACGATCT
    CAGGATTCGC ACCAAACTTC ACGCAGTGAA GTGCAAAGTC AGGGTTCTGA CCCTAAGTCT
    GAAATCTCTG AGCCAACCCA AAGGGTTTGG GCTCGATCAG ACAGTGACGA GGTGCCTCCA
    AGGGTGCCTG TGAGGACAAC ATCAAGATCT CCAGTATTGT CACGGAAAGA TTCACCACTG
    CAGGGCAGTG GGCAGCAAAA TACCCAAGCT GGTCAGAGAA ATTCCACAAG CAATATTGAG
    CCTAGACTTC TTTGGGAAAG AGTAGAGAAG CTTGTGCCCA GACCGGGTAG TGGTAGCTCG
    TCAGGATCAA GTAACTCTGG ATCCCAAGCA GGATCCCACC CAGGATCTCA GAGTGGATCT
    GGAGAGCGCT TCCGGATGAG ATCATCTTCA AAATCTGAGG GCTCACCATC TCAACGACAT
    GAAAACACAG CCAAAAAGTC TGAAGAGAAG AAAGAAGCAG TTAGACAGGC CAAAGCTCCT
    GGAGAAGTGG ATCTTACTGC ACTCGCTAAG GAGCTTCGAG CAGTAGATGA TGTTCGGCCA
    CTTCACAAAG TTACTGACTA TTCCTCATCC AGTGAAGAGT CTGGTACTAC AGATGAGGAG
    GATGATGACA TGGAACAGGA AGGAGCAGAT GAATCGACTT CAGGCCCAGA GGATGCTAGA
    GCTATGTCAT CTTTGAATTT AAGCAATGGA GAAACGGAGT CTGTGAAAAC CATGATCGTA
    CATGATGATG TCGAAAGTGA ACCTGCCATG ACCCCATCTA AAGAAGGCAC TTTAATTGTC
    CGACAGAGTA CAGTTGACAA AAAGCGTGCC AACCAACTTG AGAGCAATGG CTTTGCGAGT
    CGTATTCACC TCTTGCCAGA TCTCTTACAG CAAAGCCATT CCTCCTCCAC CACTTCTTCC
    CCAACCTCAA GTCAACCGAC ACCTACCATG TCCCCCCAGA CACCCCAGGA CAAGCTAACT
    GCTAATGAGA CTCAGTCAGC TAGTAATACT CTCCAGAAAC ACAAATCGTC CTCCTCTTTC
    ACACCGTTCA TAGATCCAAG GTTACTTCAG ATCTCTCCAT CTAGTGGAAC AACTGTTACG
    TCAGTAGGTA CATACATGCG CACACTGGAA GCTAACCTTA AAAATACTAA TTTTATTTTA
    GTGGGATTTT CCAGTGAAGC GCTAAGGCCG GATGCCATGA GGCAAGATCC TACAAGGAAA
    GGATCAGTTG TTAATGTTAA TCCCACAAAT ACTCGGCCAC AAAGTGATAC ACCAGAGATT
    CGCAAATACA AGAAGAGATT TAATTCTGAG ATTTTGTGTG CGGCATTGTG GGGTGTAAAT
    TTGCTGGTGG GAACTGAAAG TGGTTTAATG CTGTTGGATA GAAGTGGACA AGGGAAAGTG
    TACCCCCTCA TTAACAGAAG GCGATTTCAG CAGATGGATG TGCTTGAAGG CTTGAATGTC
    TTGGTAACTA TATCAGGGAA AAAGAACAAG CTGAGAGTAT ATTACTTATC CTGGCTGAGG
    AATAAAATTT TGCACAATGA TCCTGAAGTA GAGAAGAAAC AAGGATGGAC AACAGTTGGG
    GATTTGGAAG GCTGTGTGCA CTACAAAGTT GTAAAATATG AACGTATCAA GTTTTTGGTA
    ATCGCTTTAA AAAGTTCAGT GGAGGTGTAT GCCTGGGCAC CGAAACCATA CCACAAGTTT
    ATGGCCTTTA AGTCATTCGG AGATTTAGTA CACAGACCTC TCCTTGTAGA CCTGACTGTG
    GAAGAAGGAC AGAGGCTGAA AGTGATCTAT GGTTCCTGTG CTGGGTTTCA TGCAGTAGAT
    GTGGACTCGG GCGCAGTTTA TGATATTTAC TTGCCAACAC ATATTCAGAG CAGCATTCAG
    CCACACGCAA TCATCATTCT CCCAAATACT GATGGTATGG AACTGCTGGT TTGCTATGAA
    GATGAAGGAG TTTATGTTAA TACATACGGC AGGATCACCA AAGATGTAGT TCTACAATGG
    GGAGAAATGC CTACATCAGT TGCTTATATC CGATCTAACC AGATCATGGG ATGGGGTGAA
    AAAGCTATTG AGATTCGATC CGTGGAAACT GGCCACCTTG ATGGAGTATT TATGCACAAA
    AGGGCACAAA GGCTCAAGTT CTTGTGCGAA CGAAATGACA AGGTTTTCTT TGCCTCGGTG
    CGGTCTGGCG GAAGCAGCCA AGTGTATTTC ATGACTTTGG GCAGAAGCTC TCTTCTGAGC
    TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42794
    Clone ID Related Accession (Same CDS sequence) XM_018091632.1
    Accession Version XM_018091632.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4044bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCCAACG ATTCGCCTGC CAAAAGTCTT GTGGATATAG ACCTGTCGTC TCTGAGGGAC 
    CCAGCTGGCA TCTTTGAATT AGTGGAAGTG GTAGGAAATG GCACCTATGG ACAAGTATAT
    AAGGGCCGAC ATGTCAAAAC AGGCCAACTT GCAGCCATCA AAGTCATGGA TGTTACAGAG
    GATGAAGAGG AAGAAATCAA ACTGGAAATC AATATGTTGA AGAAATATTC CCACCACAGA
    AATATAGCTA CTTATTATGG TGCTTTTATT AAGAAAAGTC CTCCTGGGCA TGATGACCAG
    CTTTGGTTGG TGATGGAGTT TTGTGGTGCT GGCTCAATCA CTGACCTGGT GAAGAATACA
    AAGGGGAATA GTTTGAAAGA AGACTGGATT GCCTATATCT CTCGTGAGAT ACTTAGGGGA
    TTGGCCCACC TCCATGCTCA TCATGTGATT CACCGGGATA TCAAAGGACA GAATGTGCTG
    CTAACAGAGA ATGCCGAAGT GAAATTAGTG GATTTTGGTG TTAGTGCACA GCTGGATAGG
    ACAGTGGGCC GAAGGAACAC ATTTATTGGT ACACCATACT GGATGGCTCC AGAAGTTATC
    GCCTGTGATG AGAACCCAGA TGCTACCTAT GACTATAGGA GTGACCTCTG GTCATGTGGT
    ATTACAGCGA TTGAAATGGC AGAAGGTGCT CCTCCTCTTT GCGATATGCA TCCAATGCGA
    GCCCTGTTCC TTATCCCAAG AAATCCTCCA CCACGGCTAA AATCCAAGAA ATGGTCAAAG
    AAGTTCTTCA GCTTTATAGA AGGCTGCCTT GTGAAAAATT ACATGCAACG TCCCCCAACA
    GAACAGCTTT TAAAGCATCC ATTTATACGA GATCAGCCAA ATGAAAGGCA AGTCCGAATT
    CAGCTGAAGG ATCACATAGA CAGGACAAGG AAGAAGAGAG GAGAAAAAGA TGAAACAGAG
    TATGAATACA GTGGAAGTGA AGAGGAGGAA GAGGAAGTGC CTGAACAGGA AGGAGAACCA
    AGTTCCATTG TTAATGTGCC TGGGGAATCA ACACTCCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGCTC TGAGGCTTTG CGACGGCAAC AGCTGCTGCA GGAGCAACAA
    CTCAGAGAGC AGGAGGAATA TAAAAGACAG CTCCTGGCAG AGAGACAGAA GCGCATAGAA
    CAGCAGAAAG AGCAAAGGAG GAGACTTGAA GAGCAACAAA GAAGAGAGCG TGAGGCTCGT
    CGGCAGCAGG AACGAGAACA AAGAAGGCGA GAGCAAGAGG AAAAGAAGAG AATGGAGGAG
    ATGGAAAGAA GGCGCAAGGA AGAGGAAGAA AGGAGAAGAG CAGAGGAAGA GAAAAGAAGA
    GTGGAAAGAG AGCAGGAGTA TATCCGAAGA CAGCTGGAGG AGGAGCAGCG GCACTTAGAA
    ATCCTTCAGC AGCAGCTGCT TCAGGAGCAG GCCATGTTAC TGGAGTATAG ATGGCGTGAA
    ATGGAGGAGC ACCGCCAGGC TGAAAGACTT CAGCGTCAGT TGCAGCAAGA GCAGGCCTAT
    CTCATGTCAC TACAGCATGA TCATAGACAG CAGCAGCCAC CACAACAGCA GCCCCAGCCC
    CAGCAGCCTC CACTGTCCCA GCAGGAAAGG AGCAAGCAAA CATACCATAC CCAAGAACCC
    AAGCCTCATT ATGAACAAGC AGAACGAATA CGAGAGTGGT CTCATCTGGC ATCCCTCAAG
    AACAATGTAT CCCCTGTCTC GCGATCCCAT TCCTTCAGTG ACCCTTCTCC CAAATTTGCA
    CCTCACCTTC TACGATCTCA GGATTCGCAC CAAACTTCAC GCAGTGAAGT GCAAAGTCAG
    GGTTCTGACC CTAAGTCTGA AATCTCTGAG CCAACCCAAA GGGTTTGGGC TCGATCAGAC
    AGTGACGAGG TGCCTCCAAG GGTGCCTGTG AGGACAACAT CAAGATCTCC AGTATTGTCA
    CGGAAAGATT CACCACTGCA GGGCAGTGGG CAGCAAAATA CCCAAGCTGG TCAGAGAAAT
    TCCACAAGCA ATATTGAGCC TAGACTTCTT TGGGAAAGAG TAGAGAAGCT TGTGCCCAGA
    CCGGGTAGTG GTAGCTCGTC AGGATCAAGT AACTCTGGAT CCCAAGCAGG ATCCCACCCA
    GGATCTCAGA GTGGATCTGG AGAGCGCTTC CGGATGAGAT CATCTTCAAA ATCTGAGGGC
    TCACCATCTC AACGACATGA AAACACAGCC AAAAAGTCTG AAGAGAAGAA AGAAGCAGTT
    AGACAGGCCA AAGCTCCTGG AGAAGTGGAT CTTACTGCAC TCGCTAAGGA GCTTCGAGCA
    GTAGATGATG TTCGGCCACT TCACAAAGTT ACTGACTATT CCTCATCCAG TGAAGAGTCT
    GGTACTACAG ATGAGGAGGA TGATGACATG GAACAGGAAG GAGCAGATGA ATCGACTTCA
    GGCCCAGAGG ATGCTAGAGC TATGTCATCT TTGAATTTAA GCAATGGAGA AACGGAGTCT
    GTGAAAACCA TGATCGTACA TGATGATGTC GAAAGTGAAC CTGCCATGAC CCCATCTAAA
    GAAGGCACTT TAATTGTCCG ACAGAGTACA GTTGACAAAA AGCGTGCCAA CCAACTTGAG
    AGCAATGGCT TTGCGAGTCG TATTCACCTC TTGCCAGATC TCTTACAGCA AAGCCATTCC
    TCCTCCACCA CTTCTTCCCC AACCTCAAGT CAACCGACAC CTACCATGTC CCCCCAGACA
    CCCCAGGACA AGCTAACTGC TAATGAGACT CAGTCAGCTA GTAATACTCT CCAGAAACAC
    AAATCGTCCT CCTCTTTCAC ACCGTTCATA GATCCAAGGT TACTTCAGAT CTCTCCATCT
    AGTGGAACAA CTGTTACGTC AGTAGGTACA TACATGCGCA CACTGGAAGC TAACCTTAAA
    AATACTAATT TTATTTTAGT GGGATTTTCC AGTGAAGCGC TAAGGCCGGA TGCCATGAGG
    CAAGATCCTA CAAGGAAAGG ATCAGTTGTT AATGTTAATC CCACAAATAC TCGGCCACAA
    AGTGATACAC CAGAGATTCG CAAATACAAG AAGAGATTTA ATTCTGAGAT TTTGTGTGCG
    GCATTGTGGG GTGTAAATTT GCTGGTGGGA ACTGAAAGTG GTTTAATGCT GTTGGATAGA
    AGTGGACAAG GGAAAGTGTA CCCCCTCATT AACAGAAGGC GATTTCAGCA GATGGATGTG
    CTTGAAGGCT TGAATGTCTT GGTAACTATA TCAGGGAAAA AGAACAAGCT GAGAGTATAT
    TACTTATCCT GGCTGAGGAA TAAAATTTTG CACAATGATC CTGAAGTAGA GAAGAAACAA
    GGATGGACAA CAGTTGGGGA TTTGGAAGGC TGTGTGCACT ACAAAGTTGT AAAATATGAA
    CGTATCAAGT TTTTGGTAAT CGCTTTAAAA AGTTCAGTGG AGGTGTATGC CTGGGCACCG
    AAACCATACC ACAAGTTTAT GGCCTTTAAG TCATTCGGAG ATTTAGTACA CAGACCTCTC
    CTTGTAGACC TGACTGTGGA AGAAGGACAG AGGCTGAAAG TGATCTATGG TTCCTGTGCT
    GGGTTTCATG CAGTAGATGT GGACTCGGGC GCAGTTTATG ATATTTACTT GCCAACACAT
    ATTCAGAGCA GCATTCAGCC ACACGCAATC ATCATTCTCC CAAATACTGA TGGTATGGAA
    CTGCTGGTTT GCTATGAAGA TGAAGGAGTT TATGTTAATA CATACGGCAG GATCACCAAA
    GATGTAGTTC TACAATGGGG AGAAATGCCT ACATCAGTTG CTTATATCCG ATCTAACCAG
    ATCATGGGAT GGGGTGAAAA AGCTATTGAG ATTCGATCCG TGGAAACTGG CCACCTTGAT
    GGAGTATTTA TGCACAAAAG GGCACAAAGG CTCAAGTTCT TGTGCGAACG AAATGACAAG
    GTTTTCTTTG CCTCGGTGCG GTCTGGCGGA AGCAGCCAAG TGTATTTCAT GACTTTGGGC
    AGAAGCTCTC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947121.1
    CDS348..4391
    Translation

    Target ORF information:

    RefSeq Version XM_018091632.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 4 (map4k4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091632.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCCAACG ATTCGCCTGC CAAAAGTCTT GTGGATATAG ACCTGTCGTC TCTGAGGGAC 
    CCAGCTGGCA TCTTTGAATT AGTGGAAGTG GTAGGAAATG GCACCTATGG ACAAGTATAT
    AAGGGCCGAC ATGTCAAAAC AGGCCAACTT GCAGCCATCA AAGTCATGGA TGTTACAGAG
    GATGAAGAGG AAGAAATCAA ACTGGAAATC AATATGTTGA AGAAATATTC CCACCACAGA
    AATATAGCTA CTTATTATGG TGCTTTTATT AAGAAAAGTC CTCCTGGGCA TGATGACCAG
    CTTTGGTTGG TGATGGAGTT TTGTGGTGCT GGCTCAATCA CTGACCTGGT GAAGAATACA
    AAGGGGAATA GTTTGAAAGA AGACTGGATT GCCTATATCT CTCGTGAGAT ACTTAGGGGA
    TTGGCCCACC TCCATGCTCA TCATGTGATT CACCGGGATA TCAAAGGACA GAATGTGCTG
    CTAACAGAGA ATGCCGAAGT GAAATTAGTG GATTTTGGTG TTAGTGCACA GCTGGATAGG
    ACAGTGGGCC GAAGGAACAC ATTTATTGGT ACACCATACT GGATGGCTCC AGAAGTTATC
    GCCTGTGATG AGAACCCAGA TGCTACCTAT GACTATAGGA GTGACCTCTG GTCATGTGGT
    ATTACAGCGA TTGAAATGGC AGAAGGTGCT CCTCCTCTTT GCGATATGCA TCCAATGCGA
    GCCCTGTTCC TTATCCCAAG AAATCCTCCA CCACGGCTAA AATCCAAGAA ATGGTCAAAG
    AAGTTCTTCA GCTTTATAGA AGGCTGCCTT GTGAAAAATT ACATGCAACG TCCCCCAACA
    GAACAGCTTT TAAAGCATCC ATTTATACGA GATCAGCCAA ATGAAAGGCA AGTCCGAATT
    CAGCTGAAGG ATCACATAGA CAGGACAAGG AAGAAGAGAG GAGAAAAAGA TGAAACAGAG
    TATGAATACA GTGGAAGTGA AGAGGAGGAA GAGGAAGTGC CTGAACAGGA AGGAGAACCA
    AGTTCCATTG TTAATGTGCC TGGGGAATCA ACACTCCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGCTC TGAGGCTTTG CGACGGCAAC AGCTGCTGCA GGAGCAACAA
    CTCAGAGAGC AGGAGGAATA TAAAAGACAG CTCCTGGCAG AGAGACAGAA GCGCATAGAA
    CAGCAGAAAG AGCAAAGGAG GAGACTTGAA GAGCAACAAA GAAGAGAGCG TGAGGCTCGT
    CGGCAGCAGG AACGAGAACA AAGAAGGCGA GAGCAAGAGG AAAAGAAGAG AATGGAGGAG
    ATGGAAAGAA GGCGCAAGGA AGAGGAAGAA AGGAGAAGAG CAGAGGAAGA GAAAAGAAGA
    GTGGAAAGAG AGCAGGAGTA TATCCGAAGA CAGCTGGAGG AGGAGCAGCG GCACTTAGAA
    ATCCTTCAGC AGCAGCTGCT TCAGGAGCAG GCCATGTTAC TGGAGTATAG ATGGCGTGAA
    ATGGAGGAGC ACCGCCAGGC TGAAAGACTT CAGCGTCAGT TGCAGCAAGA GCAGGCCTAT
    CTCATGTCAC TACAGCATGA TCATAGACAG CAGCAGCCAC CACAACAGCA GCCCCAGCCC
    CAGCAGCCTC CACTGTCCCA GCAGGAAAGG AGCAAGCAAA CATACCATAC CCAAGAACCC
    AAGCCTCATT ATGAACAAGC AGAACGAATA CGAGAGTGGT CTCATCTGGC ATCCCTCAAG
    AACAATGTAT CCCCTGTCTC GCGATCCCAT TCCTTCAGTG ACCCTTCTCC CAAATTTGCA
    CCTCACCTTC TACGATCTCA GGATTCGCAC CAAACTTCAC GCAGTGAAGT GCAAAGTCAG
    GGTTCTGACC CTAAGTCTGA AATCTCTGAG CCAACCCAAA GGGTTTGGGC TCGATCAGAC
    AGTGACGAGG TGCCTCCAAG GGTGCCTGTG AGGACAACAT CAAGATCTCC AGTATTGTCA
    CGGAAAGATT CACCACTGCA GGGCAGTGGG CAGCAAAATA CCCAAGCTGG TCAGAGAAAT
    TCCACAAGCA ATATTGAGCC TAGACTTCTT TGGGAAAGAG TAGAGAAGCT TGTGCCCAGA
    CCGGGTAGTG GTAGCTCGTC AGGATCAAGT AACTCTGGAT CCCAAGCAGG ATCCCACCCA
    GGATCTCAGA GTGGATCTGG AGAGCGCTTC CGGATGAGAT CATCTTCAAA ATCTGAGGGC
    TCACCATCTC AACGACATGA AAACACAGCC AAAAAGTCTG AAGAGAAGAA AGAAGCAGTT
    AGACAGGCCA AAGCTCCTGG AGAAGTGGAT CTTACTGCAC TCGCTAAGGA GCTTCGAGCA
    GTAGATGATG TTCGGCCACT TCACAAAGTT ACTGACTATT CCTCATCCAG TGAAGAGTCT
    GGTACTACAG ATGAGGAGGA TGATGACATG GAACAGGAAG GAGCAGATGA ATCGACTTCA
    GGCCCAGAGG ATGCTAGAGC TATGTCATCT TTGAATTTAA GCAATGGAGA AACGGAGTCT
    GTGAAAACCA TGATCGTACA TGATGATGTC GAAAGTGAAC CTGCCATGAC CCCATCTAAA
    GAAGGCACTT TAATTGTCCG ACAGAGTACA GTTGACAAAA AGCGTGCCAA CCAACTTGAG
    AGCAATGGCT TTGCGAGTCG TATTCACCTC TTGCCAGATC TCTTACAGCA AAGCCATTCC
    TCCTCCACCA CTTCTTCCCC AACCTCAAGT CAACCGACAC CTACCATGTC CCCCCAGACA
    CCCCAGGACA AGCTAACTGC TAATGAGACT CAGTCAGCTA GTAATACTCT CCAGAAACAC
    AAATCGTCCT CCTCTTTCAC ACCGTTCATA GATCCAAGGT TACTTCAGAT CTCTCCATCT
    AGTGGAACAA CTGTTACGTC AGTAGGTACA TACATGCGCA CACTGGAAGC TAACCTTAAA
    AATACTAATT TTATTTTAGT GGGATTTTCC AGTGAAGCGC TAAGGCCGGA TGCCATGAGG
    CAAGATCCTA CAAGGAAAGG ATCAGTTGTT AATGTTAATC CCACAAATAC TCGGCCACAA
    AGTGATACAC CAGAGATTCG CAAATACAAG AAGAGATTTA ATTCTGAGAT TTTGTGTGCG
    GCATTGTGGG GTGTAAATTT GCTGGTGGGA ACTGAAAGTG GTTTAATGCT GTTGGATAGA
    AGTGGACAAG GGAAAGTGTA CCCCCTCATT AACAGAAGGC GATTTCAGCA GATGGATGTG
    CTTGAAGGCT TGAATGTCTT GGTAACTATA TCAGGGAAAA AGAACAAGCT GAGAGTATAT
    TACTTATCCT GGCTGAGGAA TAAAATTTTG CACAATGATC CTGAAGTAGA GAAGAAACAA
    GGATGGACAA CAGTTGGGGA TTTGGAAGGC TGTGTGCACT ACAAAGTTGT AAAATATGAA
    CGTATCAAGT TTTTGGTAAT CGCTTTAAAA AGTTCAGTGG AGGTGTATGC CTGGGCACCG
    AAACCATACC ACAAGTTTAT GGCCTTTAAG TCATTCGGAG ATTTAGTACA CAGACCTCTC
    CTTGTAGACC TGACTGTGGA AGAAGGACAG AGGCTGAAAG TGATCTATGG TTCCTGTGCT
    GGGTTTCATG CAGTAGATGT GGACTCGGGC GCAGTTTATG ATATTTACTT GCCAACACAT
    ATTCAGAGCA GCATTCAGCC ACACGCAATC ATCATTCTCC CAAATACTGA TGGTATGGAA
    CTGCTGGTTT GCTATGAAGA TGAAGGAGTT TATGTTAATA CATACGGCAG GATCACCAAA
    GATGTAGTTC TACAATGGGG AGAAATGCCT ACATCAGTTG CTTATATCCG ATCTAACCAG
    ATCATGGGAT GGGGTGAAAA AGCTATTGAG ATTCGATCCG TGGAAACTGG CCACCTTGAT
    GGAGTATTTA TGCACAAAAG GGCACAAAGG CTCAAGTTCT TGTGCGAACG AAATGACAAG
    GTTTTCTTTG CCTCGGTGCG GTCTGGCGGA AGCAGCCAAG TGTATTTCAT GACTTTGGGC
    AGAAGCTCTC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42795
    Clone ID Related Accession (Same CDS sequence) XM_018091633.1
    Accession Version XM_018091633.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3819bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCAACG ATTCGCCTGC CAAAAGTCTT GTGGATATAG ACCTGTCGTC TCTGAGGGAC 
    CCAGCTGGCA TCTTTGAATT AGTGGAAGTG GTAGGAAATG GCACCTATGG ACAAGTATAT
    AAGGGCCGAC ATGTCAAAAC AGGCCAACTT GCAGCCATCA AAGTCATGGA TGTTACAGAG
    GATGAAGAGG AAGAAATCAA ACTGGAAATC AATATGTTGA AGAAATATTC CCACCACAGA
    AATATAGCTA CTTATTATGG TGCTTTTATT AAGAAAAGTC CTCCTGGGCA TGATGACCAG
    CTTTGGTTGG TGATGGAGTT TTGTGGTGCT GGCTCAATCA CTGACCTGGT GAAGAATACA
    AAGGGGAATA GTTTGAAAGA AGACTGGATT GCCTATATCT CTCGTGAGAT ACTTAGGGGA
    TTGGCCCACC TCCATGCTCA TCATGTGATT CACCGGGATA TCAAAGGACA GAATGTGCTG
    CTAACAGAGA ATGCCGAAGT GAAATTAGTG GATTTTGGTG TTAGTGCACA GCTGGATAGG
    ACAGTGGGCC GAAGGAACAC ATTTATTGGT ACACCATACT GGATGGCTCC AGAAGTTATC
    GCCTGTGATG AGAACCCAGA TGCTACCTAT GACTATAGGA GTGACCTCTG GTCATGTGGT
    ATTACAGCGA TTGAAATGGC AGAAGGTGCT CCTCCTCTTT GCGATATGCA TCCAATGCGA
    GCCCTGTTCC TTATCCCAAG AAATCCTCCA CCACGGCTAA AATCCAAGAA ATGGTCAAAG
    AAGTTCTTCA GCTTTATAGA AGGCTGCCTT GTGAAAAATT ACATGCAACG TCCCCCAACA
    GAACAGCTTT TAAAGCATCC ATTTATACGA GATCAGCCAA ATGAAAGGCA AGTCCGAATT
    CAGCTGAAGG ATCACATAGA CAGGACAAGG AAGAAGAGAG GAGAAAAAGA TGAAACAGAG
    TATGAATACA GTGGAAGTGA AGAGGAGGAA GAGGAAGTGC CTGAACAGGA AGGAGAACCA
    AGTTCCATTG TTAATGTGCC TGGGGAATCA ACACTCCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGCTC TGAGGCTTTG CGACGGCAAC AGCTGCTGCA GGAGCAACAA
    CTCAGAGAGC AGGAGGAATA TAAAAGACAG CTCCTGGCAG AGAGACAGAA GCGCATAGAA
    CAGCAGAAAG AGCAAAGGAG GAGACTTGAA GAGCAACAAA GAAGAGAGCG TGAGGCTCGT
    CGGCAGCAGG AACGAGAACA AAGAAGGCGA GAGCAAGAGG AAAAGAAGAG AATGGAGGAG
    ATGGAAAGAA GGCGCAAGGA AGAGGAAGAA AGGAGAAGAG CAGAGGAAGA GAAAAGAAGA
    GTGGAAAGAG AGCAGGAGTA TATCCGAAGA CAGCTGGAGG AGGAGCAGCG GCACTTAGAA
    ATCCTTCAGC AGCAGCTGCT TCAGGAGCAG GCCATGTTAC TGGAGTATAG ATGGCGTGAA
    ATGGAGGAGC ACCGCCAGGC TGAAAGACTT CAGCGTCAGT TGCAGCAAGA GCAGGCCTAT
    CTCATGTCAC TACAGCATGA TCATAGACAG CAGCAGCCAC CACAACAGCA GCCCCAGCCC
    CAGCAGCCTC CACTGTCCCA GCAGGAAAGG AGCAAGCAAA CATACCATAC CCAAGAACCC
    AAGCCTCATT ATGAACAAGC AGAACGAATA CGAGAGGTGC CTGTGAGGAC AACATCAAGA
    TCTCCAGTAT TGTCACGGAA AGATTCACCA CTGCAGGGCA GTGGGCAGCA AAATACCCAA
    GCTGGTCAGA GAAATTCCAC AAGCAATATT GAGCCTAGAC TTCTTTGGGA AAGAGTAGAG
    AAGCTTGTGC CCAGACCGGG TAGTGGTAGC TCGTCAGGAT CAAGTAACTC TGGATCCCAA
    GCAGGATCCC ACCCAGGATC TCAGAGTGGA TCTGGAGAGC GCTTCCGGAT GAGATCATCT
    TCAAAATCTG AGGGCTCACC ATCTCAACGA CATGAAAACA CAGCCAAAAA GTCTGAAGAG
    AAGAAAGAAG CAGTTAGACA GGCCAAAGCT CCTGGAGAAG TGGATCTTAC TGCACTCGCT
    AAGGAGCTTC GAGCAGTAGA TGATGTTCGG CCACTTCACA AAGTTACTGA CTATTCCTCA
    TCCAGTGAAG AGTCTGGTAC TACAGATGAG GAGGATGATG ACATGGAACA GGAAGGAGCA
    GATGAATCGA CTTCAGGCCC AGAGGATGCT AGAGCTATGT CATCTTTGAA TTTAAGCAAT
    GGAGAAACGG AGTCTGTGAA AACCATGATC GTACATGATG ATGTCGAAAG TGAACCTGCC
    ATGACCCCAT CTAAAGAAGG CACTTTAATT GTCCGACAGA GTACAGTTGA CAAAAAGCGT
    GCCAACCAAC TTGAGAGCAA TGGCTTTGCG AGTCGTATTC ACCTCTTGCC AGATCTCTTA
    CAGCAAAGCC ATTCCTCCTC CACCACTTCT TCCCCAACCT CAAGTCAACC GACACCTACC
    ATGTCCCCCC AGACACCCCA GGACAAGCTA ACTGCTAATG AGACTCAGTC AGCTAGTAAT
    ACTCTCCAGA AACACAAATC GTCCTCCTCT TTCACACCGT TCATAGATCC AAGGTTACTT
    CAGATCTCTC CATCTAGTGG AACAACTGTT ACGTCAGTAG GTACATACAT GCGCACACTG
    GAAGCTAACC TTAAAAATAC TAATTTTATT TTAGTGGGAT TTTCCAGTGA AGCGCTAAGG
    CCGGATGCCA TGAGGCAAGA TCCTACAAGG AAAGGATCAG TTGTTAATGT TAATCCCACA
    AATACTCGGC CACAAAGTGA TACACCAGAG ATTCGCAAAT ACAAGAAGAG ATTTAATTCT
    GAGATTTTGT GTGCGGCATT GTGGGGTGTA AATTTGCTGG TGGGAACTGA AAGTGGTTTA
    ATGCTGTTGG ATAGAAGTGG ACAAGGGAAA GTGTACCCCC TCATTAACAG AAGGCGATTT
    CAGCAGATGG ATGTGCTTGA AGGCTTGAAT GTCTTGGTAA CTATATCAGG GAAAAAGAAC
    AAGCTGAGAG TATATTACTT ATCCTGGCTG AGGAATAAAA TTTTGCACAA TGATCCTGAA
    GTAGAGAAGA AACAAGGATG GACAACAGTT GGGGATTTGG AAGGCTGTGT GCACTACAAA
    GTTGTAAAAT ATGAACGTAT CAAGTTTTTG GTAATCGCTT TAAAAAGTTC AGTGGAGGTG
    TATGCCTGGG CACCGAAACC ATACCACAAG TTTATGGCCT TTAAGTCATT CGGAGATTTA
    GTACACAGAC CTCTCCTTGT AGACCTGACT GTGGAAGAAG GACAGAGGCT GAAAGTGATC
    TATGGTTCCT GTGCTGGGTT TCATGCAGTA GATGTGGACT CGGGCGCAGT TTATGATATT
    TACTTGCCAA CACATATTCA GAGCAGCATT CAGCCACACG CAATCATCAT TCTCCCAAAT
    ACTGATGGTA TGGAACTGCT GGTTTGCTAT GAAGATGAAG GAGTTTATGT TAATACATAC
    GGCAGGATCA CCAAAGATGT AGTTCTACAA TGGGGAGAAA TGCCTACATC AGTTGCTTAT
    ATCCGATCTA ACCAGATCAT GGGATGGGGT GAAAAAGCTA TTGAGATTCG ATCCGTGGAA
    ACTGGCCACC TTGATGGAGT ATTTATGCAC AAAAGGGCAC AAAGGCTCAA GTTCTTGTGC
    GAACGAAATG ACAAGGTTTT CTTTGCCTCG GTGCGGTCTG GCGGAAGCAG CCAAGTGTAT
    TTCATGACTT TGGGCAGAAG CTCTCTTCTG AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947122.1
    CDS348..4166
    Translation

    Target ORF information:

    RefSeq Version XM_018091633.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 4 (map4k4), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091633.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCCAACG ATTCGCCTGC CAAAAGTCTT GTGGATATAG ACCTGTCGTC TCTGAGGGAC 
    CCAGCTGGCA TCTTTGAATT AGTGGAAGTG GTAGGAAATG GCACCTATGG ACAAGTATAT
    AAGGGCCGAC ATGTCAAAAC AGGCCAACTT GCAGCCATCA AAGTCATGGA TGTTACAGAG
    GATGAAGAGG AAGAAATCAA ACTGGAAATC AATATGTTGA AGAAATATTC CCACCACAGA
    AATATAGCTA CTTATTATGG TGCTTTTATT AAGAAAAGTC CTCCTGGGCA TGATGACCAG
    CTTTGGTTGG TGATGGAGTT TTGTGGTGCT GGCTCAATCA CTGACCTGGT GAAGAATACA
    AAGGGGAATA GTTTGAAAGA AGACTGGATT GCCTATATCT CTCGTGAGAT ACTTAGGGGA
    TTGGCCCACC TCCATGCTCA TCATGTGATT CACCGGGATA TCAAAGGACA GAATGTGCTG
    CTAACAGAGA ATGCCGAAGT GAAATTAGTG GATTTTGGTG TTAGTGCACA GCTGGATAGG
    ACAGTGGGCC GAAGGAACAC ATTTATTGGT ACACCATACT GGATGGCTCC AGAAGTTATC
    GCCTGTGATG AGAACCCAGA TGCTACCTAT GACTATAGGA GTGACCTCTG GTCATGTGGT
    ATTACAGCGA TTGAAATGGC AGAAGGTGCT CCTCCTCTTT GCGATATGCA TCCAATGCGA
    GCCCTGTTCC TTATCCCAAG AAATCCTCCA CCACGGCTAA AATCCAAGAA ATGGTCAAAG
    AAGTTCTTCA GCTTTATAGA AGGCTGCCTT GTGAAAAATT ACATGCAACG TCCCCCAACA
    GAACAGCTTT TAAAGCATCC ATTTATACGA GATCAGCCAA ATGAAAGGCA AGTCCGAATT
    CAGCTGAAGG ATCACATAGA CAGGACAAGG AAGAAGAGAG GAGAAAAAGA TGAAACAGAG
    TATGAATACA GTGGAAGTGA AGAGGAGGAA GAGGAAGTGC CTGAACAGGA AGGAGAACCA
    AGTTCCATTG TTAATGTGCC TGGGGAATCA ACACTCCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGCTC TGAGGCTTTG CGACGGCAAC AGCTGCTGCA GGAGCAACAA
    CTCAGAGAGC AGGAGGAATA TAAAAGACAG CTCCTGGCAG AGAGACAGAA GCGCATAGAA
    CAGCAGAAAG AGCAAAGGAG GAGACTTGAA GAGCAACAAA GAAGAGAGCG TGAGGCTCGT
    CGGCAGCAGG AACGAGAACA AAGAAGGCGA GAGCAAGAGG AAAAGAAGAG AATGGAGGAG
    ATGGAAAGAA GGCGCAAGGA AGAGGAAGAA AGGAGAAGAG CAGAGGAAGA GAAAAGAAGA
    GTGGAAAGAG AGCAGGAGTA TATCCGAAGA CAGCTGGAGG AGGAGCAGCG GCACTTAGAA
    ATCCTTCAGC AGCAGCTGCT TCAGGAGCAG GCCATGTTAC TGGAGTATAG ATGGCGTGAA
    ATGGAGGAGC ACCGCCAGGC TGAAAGACTT CAGCGTCAGT TGCAGCAAGA GCAGGCCTAT
    CTCATGTCAC TACAGCATGA TCATAGACAG CAGCAGCCAC CACAACAGCA GCCCCAGCCC
    CAGCAGCCTC CACTGTCCCA GCAGGAAAGG AGCAAGCAAA CATACCATAC CCAAGAACCC
    AAGCCTCATT ATGAACAAGC AGAACGAATA CGAGAGGTGC CTGTGAGGAC AACATCAAGA
    TCTCCAGTAT TGTCACGGAA AGATTCACCA CTGCAGGGCA GTGGGCAGCA AAATACCCAA
    GCTGGTCAGA GAAATTCCAC AAGCAATATT GAGCCTAGAC TTCTTTGGGA AAGAGTAGAG
    AAGCTTGTGC CCAGACCGGG TAGTGGTAGC TCGTCAGGAT CAAGTAACTC TGGATCCCAA
    GCAGGATCCC ACCCAGGATC TCAGAGTGGA TCTGGAGAGC GCTTCCGGAT GAGATCATCT
    TCAAAATCTG AGGGCTCACC ATCTCAACGA CATGAAAACA CAGCCAAAAA GTCTGAAGAG
    AAGAAAGAAG CAGTTAGACA GGCCAAAGCT CCTGGAGAAG TGGATCTTAC TGCACTCGCT
    AAGGAGCTTC GAGCAGTAGA TGATGTTCGG CCACTTCACA AAGTTACTGA CTATTCCTCA
    TCCAGTGAAG AGTCTGGTAC TACAGATGAG GAGGATGATG ACATGGAACA GGAAGGAGCA
    GATGAATCGA CTTCAGGCCC AGAGGATGCT AGAGCTATGT CATCTTTGAA TTTAAGCAAT
    GGAGAAACGG AGTCTGTGAA AACCATGATC GTACATGATG ATGTCGAAAG TGAACCTGCC
    ATGACCCCAT CTAAAGAAGG CACTTTAATT GTCCGACAGA GTACAGTTGA CAAAAAGCGT
    GCCAACCAAC TTGAGAGCAA TGGCTTTGCG AGTCGTATTC ACCTCTTGCC AGATCTCTTA
    CAGCAAAGCC ATTCCTCCTC CACCACTTCT TCCCCAACCT CAAGTCAACC GACACCTACC
    ATGTCCCCCC AGACACCCCA GGACAAGCTA ACTGCTAATG AGACTCAGTC AGCTAGTAAT
    ACTCTCCAGA AACACAAATC GTCCTCCTCT TTCACACCGT TCATAGATCC AAGGTTACTT
    CAGATCTCTC CATCTAGTGG AACAACTGTT ACGTCAGTAG GTACATACAT GCGCACACTG
    GAAGCTAACC TTAAAAATAC TAATTTTATT TTAGTGGGAT TTTCCAGTGA AGCGCTAAGG
    CCGGATGCCA TGAGGCAAGA TCCTACAAGG AAAGGATCAG TTGTTAATGT TAATCCCACA
    AATACTCGGC CACAAAGTGA TACACCAGAG ATTCGCAAAT ACAAGAAGAG ATTTAATTCT
    GAGATTTTGT GTGCGGCATT GTGGGGTGTA AATTTGCTGG TGGGAACTGA AAGTGGTTTA
    ATGCTGTTGG ATAGAAGTGG ACAAGGGAAA GTGTACCCCC TCATTAACAG AAGGCGATTT
    CAGCAGATGG ATGTGCTTGA AGGCTTGAAT GTCTTGGTAA CTATATCAGG GAAAAAGAAC
    AAGCTGAGAG TATATTACTT ATCCTGGCTG AGGAATAAAA TTTTGCACAA TGATCCTGAA
    GTAGAGAAGA AACAAGGATG GACAACAGTT GGGGATTTGG AAGGCTGTGT GCACTACAAA
    GTTGTAAAAT ATGAACGTAT CAAGTTTTTG GTAATCGCTT TAAAAAGTTC AGTGGAGGTG
    TATGCCTGGG CACCGAAACC ATACCACAAG TTTATGGCCT TTAAGTCATT CGGAGATTTA
    GTACACAGAC CTCTCCTTGT AGACCTGACT GTGGAAGAAG GACAGAGGCT GAAAGTGATC
    TATGGTTCCT GTGCTGGGTT TCATGCAGTA GATGTGGACT CGGGCGCAGT TTATGATATT
    TACTTGCCAA CACATATTCA GAGCAGCATT CAGCCACACG CAATCATCAT TCTCCCAAAT
    ACTGATGGTA TGGAACTGCT GGTTTGCTAT GAAGATGAAG GAGTTTATGT TAATACATAC
    GGCAGGATCA CCAAAGATGT AGTTCTACAA TGGGGAGAAA TGCCTACATC AGTTGCTTAT
    ATCCGATCTA ACCAGATCAT GGGATGGGGT GAAAAAGCTA TTGAGATTCG ATCCGTGGAA
    ACTGGCCACC TTGATGGAGT ATTTATGCAC AAAAGGGCAC AAAGGCTCAA GTTCTTGTGC
    GAACGAAATG ACAAGGTTTT CTTTGCCTCG GTGCGGTCTG GCGGAAGCAG CCAAGTGTAT
    TTCATGACTT TGGGCAGAAG CTCTCTTCTG AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42796
    Clone ID Related Accession (Same CDS sequence) XM_018091634.1
    Accession Version XM_018091634.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3765bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCCAACG ATTCGCCTGC CAAAAGTCTT GTGGATATAG ACCTGTCGTC TCTGAGGGAC 
    CCAGCTGGCA TCTTTGAATT AGTGGAAGTG GTAGGAAATG GCACCTATGG ACAAGTATAT
    AAGGGCCGAC ATGTCAAAAC AGGCCAACTT GCAGCCATCA AAGTCATGGA TGTTACAGAG
    GATGAAGAGG AAGAAATCAA ACTGGAAATC AATATGTTGA AGAAATATTC CCACCACAGA
    AATATAGCTA CTTATTATGG TGCTTTTATT AAGAAAAGTC CTCCTGGGCA TGATGACCAG
    CTTTGGTTGG TGATGGAGTT TTGTGGTGCT GGCTCAATCA CTGACCTGGT GAAGAATACA
    AAGGGGAATA GTTTGAAAGA AGACTGGATT GCCTATATCT CTCGTGAGAT ACTTAGGGGA
    TTGGCCCACC TCCATGCTCA TCATGTGATT CACCGGGATA TCAAAGGACA GAATGTGCTG
    CTAACAGAGA ATGCCGAAGT GAAATTAGTG GATTTTGGTG TTAGTGCACA GCTGGATAGG
    ACAGTGGGCC GAAGGAACAC ATTTATTGGT ACACCATACT GGATGGCTCC AGAAGTTATC
    GCCTGTGATG AGAACCCAGA TGCTACCTAT GACTATAGGA GTGACCTCTG GTCATGTGGT
    ATTACAGCGA TTGAAATGGC AGAAGGTGCT CCTCCTCTTT GCGATATGCA TCCAATGCGA
    GCCCTGTTCC TTATCCCAAG AAATCCTCCA CCACGGCTAA AATCCAAGAA ATGGTCAAAG
    AAGTTCTTCA GCTTTATAGA AGGCTGCCTT GTGAAAAATT ACATGCAACG TCCCCCAACA
    GAACAGCTTT TAAAGCATCC ATTTATACGA GATCAGCCAA ATGAAAGGCA AGTCCGAATT
    CAGCTGAAGG ATCACATAGA CAGGACAAGG AAGAAGAGAG GAGAAAAAGA TGAAACAGAG
    TATGAATACA GTGGAAGTGA AGAGGAGGAA GAGGAAGTGC CTGAACAGGA AGGAGAACCA
    AGTTCCATTG TTAATGTGCC TGGGGAATCA ACACTCCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGCTC TGAGGCTTTG CGACGGCAAC AGCTGCTGCA GGAGCAACAA
    CTCAGAGAGC AGGAGGAATA TAAAAGACAG CTCCTGGCAG AGAGACAGAA GCGCATAGAA
    CAGCAGAAAG AGCAAAGGAG GAGACTTGAA GAGCAACAAA GAAGAGAGCG TGAGGCTCGT
    CGGCAGCAGG AACGAGAACA AAGAAGGCGA GAGCAAGAGG AAAAGAAGAG AATGGAGGAG
    ATGGAAAGAA GGCGCAAGGA AGAGGAAGAA AGGAGAAGAG CAGAGGAAGA GAAAAGAAGA
    GTGGAAAGAG AGCAGGAGTA TATCCGAAGA CAGCTGGAGG AGGAGCAGCG GCACTTAGAA
    ATCCTTCAGC AGCAGCTGCT TCAGGAGCAG GCCATGTTAC TGGAGTATAG ATGGCGTGAA
    ATGGAGGAGC ACCGCCAGGC TGAAAGACTT CAGCGTCAGT TGCAGCAAGA GCAGGCCTAT
    CTCATGTCAC TACAGCATGA TCATAGACAG CAGCAGCCAC CACAACAGCA GCCCCAGCCC
    CAGCAGCCTC CACTGTCCCA GCAGGAAAGG AGCAAGCAAA CATACCATAC CCAAGAACCC
    AAGCCTCATT ATGAACAAGC AGAACGAATA CGAGAGGTGC CTGTGAGGAC AACATCAAGA
    TCTCCAGTAT TGTCACGGAA AGATTCACCA CTGCAGGGCA GTGGGCAGCA AAATACCCAA
    GCTGGTCAGA GAAATTCCAC AAGCAATATT GAGCCTAGAC TTCTTTGGGA AAGAGTAGAG
    AAGCTTGTGC CCAGACCGGG TAGTGGTAGC TCGTCAGGAT CAAGTAACTC TGGATCCCAA
    GCAGGATCCC ACCCAGGATC TCAGAGTGGA TCTGGAGAGC GCTTCCGGAT GAGATCATCT
    TCAAAATCTG AGGGCTCACC ATCTCAACGA CATGAAAACA CAGCCAAAAA GTCTGAAGAG
    AAGAAAGAAG CAGTTAGACA GGCCAAAGCT CCTGGAGAAG TGGATCTTAC TGCACTCGCT
    AAGGAGCTTC GAGCAGTAGA TGATGTTCGG CCACTTCACA AAGTTACTGA CTATTCCTCA
    TCCAGTGAAG AGTCTGGTAC TACAGATGAG GAGGATGATG ACATGGAACA GGAAGGAGCA
    GATGAATCGA CTTCAGGCCC AGAGGATGCT AGAGCTATGT CATCTTTGAA TTTAAGCAAT
    GGAGAAACGG AGTCTGTGAA AACCATGATC GTACATGATG ATGTCGAAAG TGAACCTGCC
    ATGACCCCAT CTAAAGAAGG CACTTTAATT GTCCGACAGA GTACAGTTGA CAAAAAGCGT
    GCCAACCAAC TTGAGAGCAA TGGCTTTGCG AGTCGTATTC ACCTCTTGCC AGATCTCTTA
    CAGCAAAGCC ATTCCTCCTC CACCACTTCT TCCCCAACCT CAAGTCAACC GACACCTACC
    ATGTCCCCCC AGACACCCCA GGACAAGCTA ACTGCTAATG AGACTCAGTC AGCTAGTAAT
    ACTCTCCAGA AACACAAATC GTCCTCCTCT TTCACACCGT TCATAGATCC AAGGTTACTT
    CAGATCTCTC CATCTAGTGG AACAACTGTT ACGTCAGTAG TGGGATTTTC CAGTGAAGCG
    CTAAGGCCGG ATGCCATGAG GCAAGATCCT ACAAGGAAAG GATCAGTTGT TAATGTTAAT
    CCCACAAATA CTCGGCCACA AAGTGATACA CCAGAGATTC GCAAATACAA GAAGAGATTT
    AATTCTGAGA TTTTGTGTGC GGCATTGTGG GGTGTAAATT TGCTGGTGGG AACTGAAAGT
    GGTTTAATGC TGTTGGATAG AAGTGGACAA GGGAAAGTGT ACCCCCTCAT TAACAGAAGG
    CGATTTCAGC AGATGGATGT GCTTGAAGGC TTGAATGTCT TGGTAACTAT ATCAGGGAAA
    AAGAACAAGC TGAGAGTATA TTACTTATCC TGGCTGAGGA ATAAAATTTT GCACAATGAT
    CCTGAAGTAG AGAAGAAACA AGGATGGACA ACAGTTGGGG ATTTGGAAGG CTGTGTGCAC
    TACAAAGTTG TAAAATATGA ACGTATCAAG TTTTTGGTAA TCGCTTTAAA AAGTTCAGTG
    GAGGTGTATG CCTGGGCACC GAAACCATAC CACAAGTTTA TGGCCTTTAA GTCATTCGGA
    GATTTAGTAC ACAGACCTCT CCTTGTAGAC CTGACTGTGG AAGAAGGACA GAGGCTGAAA
    GTGATCTATG GTTCCTGTGC TGGGTTTCAT GCAGTAGATG TGGACTCGGG CGCAGTTTAT
    GATATTTACT TGCCAACACA TATTCAGAGC AGCATTCAGC CACACGCAAT CATCATTCTC
    CCAAATACTG ATGGTATGGA ACTGCTGGTT TGCTATGAAG ATGAAGGAGT TTATGTTAAT
    ACATACGGCA GGATCACCAA AGATGTAGTT CTACAATGGG GAGAAATGCC TACATCAGTT
    GCTTATATCC GATCTAACCA GATCATGGGA TGGGGTGAAA AAGCTATTGA GATTCGATCC
    GTGGAAACTG GCCACCTTGA TGGAGTATTT ATGCACAAAA GGGCACAAAG GCTCAAGTTC
    TTGTGCGAAC GAAATGACAA GGTTTTCTTT GCCTCGGTGC GGTCTGGCGG AAGCAGCCAA
    GTGTATTTCA TGACTTTGGG CAGAAGCTCT CTTCTGAGCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947123.1
    CDS348..4112
    Translation

    Target ORF information:

    RefSeq Version XM_018091634.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 4 (map4k4), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091634.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCCAACG ATTCGCCTGC CAAAAGTCTT GTGGATATAG ACCTGTCGTC TCTGAGGGAC 
    CCAGCTGGCA TCTTTGAATT AGTGGAAGTG GTAGGAAATG GCACCTATGG ACAAGTATAT
    AAGGGCCGAC ATGTCAAAAC AGGCCAACTT GCAGCCATCA AAGTCATGGA TGTTACAGAG
    GATGAAGAGG AAGAAATCAA ACTGGAAATC AATATGTTGA AGAAATATTC CCACCACAGA
    AATATAGCTA CTTATTATGG TGCTTTTATT AAGAAAAGTC CTCCTGGGCA TGATGACCAG
    CTTTGGTTGG TGATGGAGTT TTGTGGTGCT GGCTCAATCA CTGACCTGGT GAAGAATACA
    AAGGGGAATA GTTTGAAAGA AGACTGGATT GCCTATATCT CTCGTGAGAT ACTTAGGGGA
    TTGGCCCACC TCCATGCTCA TCATGTGATT CACCGGGATA TCAAAGGACA GAATGTGCTG
    CTAACAGAGA ATGCCGAAGT GAAATTAGTG GATTTTGGTG TTAGTGCACA GCTGGATAGG
    ACAGTGGGCC GAAGGAACAC ATTTATTGGT ACACCATACT GGATGGCTCC AGAAGTTATC
    GCCTGTGATG AGAACCCAGA TGCTACCTAT GACTATAGGA GTGACCTCTG GTCATGTGGT
    ATTACAGCGA TTGAAATGGC AGAAGGTGCT CCTCCTCTTT GCGATATGCA TCCAATGCGA
    GCCCTGTTCC TTATCCCAAG AAATCCTCCA CCACGGCTAA AATCCAAGAA ATGGTCAAAG
    AAGTTCTTCA GCTTTATAGA AGGCTGCCTT GTGAAAAATT ACATGCAACG TCCCCCAACA
    GAACAGCTTT TAAAGCATCC ATTTATACGA GATCAGCCAA ATGAAAGGCA AGTCCGAATT
    CAGCTGAAGG ATCACATAGA CAGGACAAGG AAGAAGAGAG GAGAAAAAGA TGAAACAGAG
    TATGAATACA GTGGAAGTGA AGAGGAGGAA GAGGAAGTGC CTGAACAGGA AGGAGAACCA
    AGTTCCATTG TTAATGTGCC TGGGGAATCA ACACTCCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGCTC TGAGGCTTTG CGACGGCAAC AGCTGCTGCA GGAGCAACAA
    CTCAGAGAGC AGGAGGAATA TAAAAGACAG CTCCTGGCAG AGAGACAGAA GCGCATAGAA
    CAGCAGAAAG AGCAAAGGAG GAGACTTGAA GAGCAACAAA GAAGAGAGCG TGAGGCTCGT
    CGGCAGCAGG AACGAGAACA AAGAAGGCGA GAGCAAGAGG AAAAGAAGAG AATGGAGGAG
    ATGGAAAGAA GGCGCAAGGA AGAGGAAGAA AGGAGAAGAG CAGAGGAAGA GAAAAGAAGA
    GTGGAAAGAG AGCAGGAGTA TATCCGAAGA CAGCTGGAGG AGGAGCAGCG GCACTTAGAA
    ATCCTTCAGC AGCAGCTGCT TCAGGAGCAG GCCATGTTAC TGGAGTATAG ATGGCGTGAA
    ATGGAGGAGC ACCGCCAGGC TGAAAGACTT CAGCGTCAGT TGCAGCAAGA GCAGGCCTAT
    CTCATGTCAC TACAGCATGA TCATAGACAG CAGCAGCCAC CACAACAGCA GCCCCAGCCC
    CAGCAGCCTC CACTGTCCCA GCAGGAAAGG AGCAAGCAAA CATACCATAC CCAAGAACCC
    AAGCCTCATT ATGAACAAGC AGAACGAATA CGAGAGGTGC CTGTGAGGAC AACATCAAGA
    TCTCCAGTAT TGTCACGGAA AGATTCACCA CTGCAGGGCA GTGGGCAGCA AAATACCCAA
    GCTGGTCAGA GAAATTCCAC AAGCAATATT GAGCCTAGAC TTCTTTGGGA AAGAGTAGAG
    AAGCTTGTGC CCAGACCGGG TAGTGGTAGC TCGTCAGGAT CAAGTAACTC TGGATCCCAA
    GCAGGATCCC ACCCAGGATC TCAGAGTGGA TCTGGAGAGC GCTTCCGGAT GAGATCATCT
    TCAAAATCTG AGGGCTCACC ATCTCAACGA CATGAAAACA CAGCCAAAAA GTCTGAAGAG
    AAGAAAGAAG CAGTTAGACA GGCCAAAGCT CCTGGAGAAG TGGATCTTAC TGCACTCGCT
    AAGGAGCTTC GAGCAGTAGA TGATGTTCGG CCACTTCACA AAGTTACTGA CTATTCCTCA
    TCCAGTGAAG AGTCTGGTAC TACAGATGAG GAGGATGATG ACATGGAACA GGAAGGAGCA
    GATGAATCGA CTTCAGGCCC AGAGGATGCT AGAGCTATGT CATCTTTGAA TTTAAGCAAT
    GGAGAAACGG AGTCTGTGAA AACCATGATC GTACATGATG ATGTCGAAAG TGAACCTGCC
    ATGACCCCAT CTAAAGAAGG CACTTTAATT GTCCGACAGA GTACAGTTGA CAAAAAGCGT
    GCCAACCAAC TTGAGAGCAA TGGCTTTGCG AGTCGTATTC ACCTCTTGCC AGATCTCTTA
    CAGCAAAGCC ATTCCTCCTC CACCACTTCT TCCCCAACCT CAAGTCAACC GACACCTACC
    ATGTCCCCCC AGACACCCCA GGACAAGCTA ACTGCTAATG AGACTCAGTC AGCTAGTAAT
    ACTCTCCAGA AACACAAATC GTCCTCCTCT TTCACACCGT TCATAGATCC AAGGTTACTT
    CAGATCTCTC CATCTAGTGG AACAACTGTT ACGTCAGTAG TGGGATTTTC CAGTGAAGCG
    CTAAGGCCGG ATGCCATGAG GCAAGATCCT ACAAGGAAAG GATCAGTTGT TAATGTTAAT
    CCCACAAATA CTCGGCCACA AAGTGATACA CCAGAGATTC GCAAATACAA GAAGAGATTT
    AATTCTGAGA TTTTGTGTGC GGCATTGTGG GGTGTAAATT TGCTGGTGGG AACTGAAAGT
    GGTTTAATGC TGTTGGATAG AAGTGGACAA GGGAAAGTGT ACCCCCTCAT TAACAGAAGG
    CGATTTCAGC AGATGGATGT GCTTGAAGGC TTGAATGTCT TGGTAACTAT ATCAGGGAAA
    AAGAACAAGC TGAGAGTATA TTACTTATCC TGGCTGAGGA ATAAAATTTT GCACAATGAT
    CCTGAAGTAG AGAAGAAACA AGGATGGACA ACAGTTGGGG ATTTGGAAGG CTGTGTGCAC
    TACAAAGTTG TAAAATATGA ACGTATCAAG TTTTTGGTAA TCGCTTTAAA AAGTTCAGTG
    GAGGTGTATG CCTGGGCACC GAAACCATAC CACAAGTTTA TGGCCTTTAA GTCATTCGGA
    GATTTAGTAC ACAGACCTCT CCTTGTAGAC CTGACTGTGG AAGAAGGACA GAGGCTGAAA
    GTGATCTATG GTTCCTGTGC TGGGTTTCAT GCAGTAGATG TGGACTCGGG CGCAGTTTAT
    GATATTTACT TGCCAACACA TATTCAGAGC AGCATTCAGC CACACGCAAT CATCATTCTC
    CCAAATACTG ATGGTATGGA ACTGCTGGTT TGCTATGAAG ATGAAGGAGT TTATGTTAAT
    ACATACGGCA GGATCACCAA AGATGTAGTT CTACAATGGG GAGAAATGCC TACATCAGTT
    GCTTATATCC GATCTAACCA GATCATGGGA TGGGGTGAAA AAGCTATTGA GATTCGATCC
    GTGGAAACTG GCCACCTTGA TGGAGTATTT ATGCACAAAA GGGCACAAAG GCTCAAGTTC
    TTGTGCGAAC GAAATGACAA GGTTTTCTTT GCCTCGGTGC GGTCTGGCGG AAGCAGCCAA
    GTGTATTTCA TGACTTTGGG CAGAAGCTCT CTTCTGAGCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42797
    Clone ID Related Accession (Same CDS sequence) XM_018091635.1
    Accession Version XM_018091635.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3582bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCAACG ATTCGCCTGC CAAAAGTCTT GTGGATATAG ACCTGTCGTC TCTGAGGGAC 
    CCAGCTGGCA TCTTTGAATT AGTGGAAGTG GTAGGAAATG GCACCTATGG ACAAGTATAT
    AAGGGCCGAC ATGTCAAAAC AGGCCAACTT GCAGCCATCA AAGTCATGGA TGTTACAGAG
    GATGAAGAGG AAGAAATCAA ACTGGAAATC AATATGTTGA AGAAATATTC CCACCACAGA
    AATATAGCTA CTTATTATGG TGCTTTTATT AAGAAAAGTC CTCCTGGGCA TGATGACCAG
    CTTTGGTTGG TGATGGAGTT TTGTGGTGCT GGCTCAATCA CTGACCTGGT GAAGAATACA
    AAGGGGAATA GTTTGAAAGA AGACTGGATT GCCTATATCT CTCGTGAGAT ACTTAGGGGA
    TTGGCCCACC TCCATGCTCA TCATGTGATT CACCGGGATA TCAAAGGACA GAATGTGCTG
    CTAACAGAGA ATGCCGAAGT GAAATTAGTG GATTTTGGTG TTAGTGCACA GCTGGATAGG
    ACAGTGGGCC GAAGGAACAC ATTTATTGGT ACACCATACT GGATGGCTCC AGAAGTTATC
    GCCTGTGATG AGAACCCAGA TGCTACCTAT GACTATAGGA GTGACCTCTG GTCATGTGGT
    ATTACAGCGA TTGAAATGGC AGAAGGTGCT CCTCCTCTTT GCGATATGCA TCCAATGCGA
    GCCCTGTTCC TTATCCCAAG AAATCCTCCA CCACGGCTAA AATCCAAGAA ATGGTCAAAG
    AAGTTCTTCA GCTTTATAGA AGGCTGCCTT GTGAAAAATT ACATGCAACG TCCCCCAACA
    GAACAGCTTT TAAAGCATCC ATTTATACGA GATCAGCCAA ATGAAAGGCA AGTCCGAATT
    CAGCTGAAGG ATCACATAGA CAGGACAAGG AAGAAGAGAG GAGAAAAAGA TGAAACAGAG
    TATGAATACA GTGGAAGTGA AGAGGAGGAA GAGGAAGTGC CTGAACAGGA AGGAGAACCA
    AGTTCCATTG TTAATGTGCC TGGGGAATCA ACACTCCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGCTC TGAGGCTTTG CGACGGCAAC AGCTGCTGCA GGAGCAACAA
    CTCAGAGAGC AGGAGGAATA TAAAAGACAG CTCCTGGCAG AGAGACAGAA GCGCATAGAA
    CAGCAGAAAG AGCAAAGGAG GAGACTTGAA GAGCAACAAA GAAGAGAGCG TGAGGCTCGT
    CGGCAGCAGG AACGAGAACA AAGAAGGCGA GAGCAAGAGG AAAAGAAGAG AATGGAGGAG
    ATGGAAAGAA GGCGCAAGGA AGAGGAAGAA AGGAGAAGAG CAGAGGAAGA GAAAAGAAGA
    GTGGAAAGAG AGCAGGAGTA TATCCGAAGA CAGCTGGAGG AGGAGCAGCG GCACTTAGAA
    ATCCTTCAGC AGCAGCTGCT TCAGGAGCAG GCCATGTTAC TGGAGTATAG ATGGCGTGAA
    ATGGAGGAGC ACCGCCAGGC TGAAAGACTT CAGCGTCAGT TGCAGCAAGA GCAGGCCTAT
    CTCATGTCAC TACAGCATGA TCATAGACAG CAGCAGCCAC CACAACAGCA GCCCCAGCCC
    CAGCAGCCTC CACTGTCCCA GCAGGAAAGG AGCAAGCAAA CATACCATAC CCAAGAACCC
    AAGCCTCATT ATGAACAAGC AGAACGAATA CGAGAGGTGC CTGTGAGGAC AACATCAAGA
    TCTCCAGTAT TGTCACGGAA AGATTCACCA CTGCAGGGCA GTGGGCAGCA AAATACCCAA
    GCTGGTCAGA GAAATTCCAC AAGCAATATT GAGCCTAGAC TTCTTTGGGA AAGAGTAGAG
    AAGCTTGTGC CCAGACCGGG TAGTGGTAGC TCGTCAGGAT CAAGTAACTC TGGATCCCAA
    GCAGGATCCC ACCCAGGATC TCAGAGTGGA TCTGGAGAGC GCTTCCGGAT GAGATCATCT
    TCAAAATCTG AGGGCTCACC ATCTCAACGA CATGAAAACA CAGCCAAAAA GTCTGAAGAG
    AAGAAAGAAG CAGTTAGACA GGCCAAAGCT CCTGGAGAAG TGGATCTTAC TGCACTCGCT
    AAGGAGCTTC GAGCAGTAGA TGATGTTCGG CCACTTCACA AAGTTACTGA CTATTCCTCA
    TCCAGTGAAG AGTCTGGTAC TACAGATGAG GAGGATGATG ACATGGAACA GGAAGGAGCA
    GATGAATCGA CTTCAGGCCC AGAGGATGCT AGAGCTATGT CATCTTTGAA TTTAAGCAAT
    GGAGAAACGG AGTCTGTGAA AACCATGATC GTACATGATG ATGTCGAAAG TGAACCTGCC
    ATGACCCCAT CTAAAGAAGG CACTTTAATT GTCCGACAGA CTCAGTCAGC TAGTAATACT
    CTCCAGAAAC ACAAATCGTC CTCCTCTTTC ACACCGTTCA TAGATCCAAG GTTACTTCAG
    ATCTCTCCAT CTAGTGGAAC AACTGTTACG TCAGTAGTGG GATTTTCCAG TGAAGCGCTA
    AGGCCGGATG CCATGAGGCA AGATCCTACA AGGAAAGGAT CAGTTGTTAA TGTTAATCCC
    ACAAATACTC GGCCACAAAG TGATACACCA GAGATTCGCA AATACAAGAA GAGATTTAAT
    TCTGAGATTT TGTGTGCGGC ATTGTGGGGT GTAAATTTGC TGGTGGGAAC TGAAAGTGGT
    TTAATGCTGT TGGATAGAAG TGGACAAGGG AAAGTGTACC CCCTCATTAA CAGAAGGCGA
    TTTCAGCAGA TGGATGTGCT TGAAGGCTTG AATGTCTTGG TAACTATATC AGGGAAAAAG
    AACAAGCTGA GAGTATATTA CTTATCCTGG CTGAGGAATA AAATTTTGCA CAATGATCCT
    GAAGTAGAGA AGAAACAAGG ATGGACAACA GTTGGGGATT TGGAAGGCTG TGTGCACTAC
    AAAGTTGTAA AATATGAACG TATCAAGTTT TTGGTAATCG CTTTAAAAAG TTCAGTGGAG
    GTGTATGCCT GGGCACCGAA ACCATACCAC AAGTTTATGG CCTTTAAGTC ATTCGGAGAT
    TTAGTACACA GACCTCTCCT TGTAGACCTG ACTGTGGAAG AAGGACAGAG GCTGAAAGTG
    ATCTATGGTT CCTGTGCTGG GTTTCATGCA GTAGATGTGG ACTCGGGCGC AGTTTATGAT
    ATTTACTTGC CAACACATAT TCAGAGCAGC ATTCAGCCAC ACGCAATCAT CATTCTCCCA
    AATACTGATG GTATGGAACT GCTGGTTTGC TATGAAGATG AAGGAGTTTA TGTTAATACA
    TACGGCAGGA TCACCAAAGA TGTAGTTCTA CAATGGGGAG AAATGCCTAC ATCAGTTGCT
    TATATCCGAT CTAACCAGAT CATGGGATGG GGTGAAAAAG CTATTGAGAT TCGATCCGTG
    GAAACTGGCC ACCTTGATGG AGTATTTATG CACAAAAGGG CACAAAGGCT CAAGTTCTTG
    TGCGAACGAA ATGACAAGGT TTTCTTTGCC TCGGTGCGGT CTGGCGGAAG CAGCCAAGTG
    TATTTCATGA CTTTGGGCAG AAGCTCTCTT CTGAGCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947124.1
    CDS348..3929
    Translation

    Target ORF information:

    RefSeq Version XM_018091635.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 4 (map4k4), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091635.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCAACG ATTCGCCTGC CAAAAGTCTT GTGGATATAG ACCTGTCGTC TCTGAGGGAC 
    CCAGCTGGCA TCTTTGAATT AGTGGAAGTG GTAGGAAATG GCACCTATGG ACAAGTATAT
    AAGGGCCGAC ATGTCAAAAC AGGCCAACTT GCAGCCATCA AAGTCATGGA TGTTACAGAG
    GATGAAGAGG AAGAAATCAA ACTGGAAATC AATATGTTGA AGAAATATTC CCACCACAGA
    AATATAGCTA CTTATTATGG TGCTTTTATT AAGAAAAGTC CTCCTGGGCA TGATGACCAG
    CTTTGGTTGG TGATGGAGTT TTGTGGTGCT GGCTCAATCA CTGACCTGGT GAAGAATACA
    AAGGGGAATA GTTTGAAAGA AGACTGGATT GCCTATATCT CTCGTGAGAT ACTTAGGGGA
    TTGGCCCACC TCCATGCTCA TCATGTGATT CACCGGGATA TCAAAGGACA GAATGTGCTG
    CTAACAGAGA ATGCCGAAGT GAAATTAGTG GATTTTGGTG TTAGTGCACA GCTGGATAGG
    ACAGTGGGCC GAAGGAACAC ATTTATTGGT ACACCATACT GGATGGCTCC AGAAGTTATC
    GCCTGTGATG AGAACCCAGA TGCTACCTAT GACTATAGGA GTGACCTCTG GTCATGTGGT
    ATTACAGCGA TTGAAATGGC AGAAGGTGCT CCTCCTCTTT GCGATATGCA TCCAATGCGA
    GCCCTGTTCC TTATCCCAAG AAATCCTCCA CCACGGCTAA AATCCAAGAA ATGGTCAAAG
    AAGTTCTTCA GCTTTATAGA AGGCTGCCTT GTGAAAAATT ACATGCAACG TCCCCCAACA
    GAACAGCTTT TAAAGCATCC ATTTATACGA GATCAGCCAA ATGAAAGGCA AGTCCGAATT
    CAGCTGAAGG ATCACATAGA CAGGACAAGG AAGAAGAGAG GAGAAAAAGA TGAAACAGAG
    TATGAATACA GTGGAAGTGA AGAGGAGGAA GAGGAAGTGC CTGAACAGGA AGGAGAACCA
    AGTTCCATTG TTAATGTGCC TGGGGAATCA ACACTCCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGCTC TGAGGCTTTG CGACGGCAAC AGCTGCTGCA GGAGCAACAA
    CTCAGAGAGC AGGAGGAATA TAAAAGACAG CTCCTGGCAG AGAGACAGAA GCGCATAGAA
    CAGCAGAAAG AGCAAAGGAG GAGACTTGAA GAGCAACAAA GAAGAGAGCG TGAGGCTCGT
    CGGCAGCAGG AACGAGAACA AAGAAGGCGA GAGCAAGAGG AAAAGAAGAG AATGGAGGAG
    ATGGAAAGAA GGCGCAAGGA AGAGGAAGAA AGGAGAAGAG CAGAGGAAGA GAAAAGAAGA
    GTGGAAAGAG AGCAGGAGTA TATCCGAAGA CAGCTGGAGG AGGAGCAGCG GCACTTAGAA
    ATCCTTCAGC AGCAGCTGCT TCAGGAGCAG GCCATGTTAC TGGAGTATAG ATGGCGTGAA
    ATGGAGGAGC ACCGCCAGGC TGAAAGACTT CAGCGTCAGT TGCAGCAAGA GCAGGCCTAT
    CTCATGTCAC TACAGCATGA TCATAGACAG CAGCAGCCAC CACAACAGCA GCCCCAGCCC
    CAGCAGCCTC CACTGTCCCA GCAGGAAAGG AGCAAGCAAA CATACCATAC CCAAGAACCC
    AAGCCTCATT ATGAACAAGC AGAACGAATA CGAGAGGTGC CTGTGAGGAC AACATCAAGA
    TCTCCAGTAT TGTCACGGAA AGATTCACCA CTGCAGGGCA GTGGGCAGCA AAATACCCAA
    GCTGGTCAGA GAAATTCCAC AAGCAATATT GAGCCTAGAC TTCTTTGGGA AAGAGTAGAG
    AAGCTTGTGC CCAGACCGGG TAGTGGTAGC TCGTCAGGAT CAAGTAACTC TGGATCCCAA
    GCAGGATCCC ACCCAGGATC TCAGAGTGGA TCTGGAGAGC GCTTCCGGAT GAGATCATCT
    TCAAAATCTG AGGGCTCACC ATCTCAACGA CATGAAAACA CAGCCAAAAA GTCTGAAGAG
    AAGAAAGAAG CAGTTAGACA GGCCAAAGCT CCTGGAGAAG TGGATCTTAC TGCACTCGCT
    AAGGAGCTTC GAGCAGTAGA TGATGTTCGG CCACTTCACA AAGTTACTGA CTATTCCTCA
    TCCAGTGAAG AGTCTGGTAC TACAGATGAG GAGGATGATG ACATGGAACA GGAAGGAGCA
    GATGAATCGA CTTCAGGCCC AGAGGATGCT AGAGCTATGT CATCTTTGAA TTTAAGCAAT
    GGAGAAACGG AGTCTGTGAA AACCATGATC GTACATGATG ATGTCGAAAG TGAACCTGCC
    ATGACCCCAT CTAAAGAAGG CACTTTAATT GTCCGACAGA CTCAGTCAGC TAGTAATACT
    CTCCAGAAAC ACAAATCGTC CTCCTCTTTC ACACCGTTCA TAGATCCAAG GTTACTTCAG
    ATCTCTCCAT CTAGTGGAAC AACTGTTACG TCAGTAGTGG GATTTTCCAG TGAAGCGCTA
    AGGCCGGATG CCATGAGGCA AGATCCTACA AGGAAAGGAT CAGTTGTTAA TGTTAATCCC
    ACAAATACTC GGCCACAAAG TGATACACCA GAGATTCGCA AATACAAGAA GAGATTTAAT
    TCTGAGATTT TGTGTGCGGC ATTGTGGGGT GTAAATTTGC TGGTGGGAAC TGAAAGTGGT
    TTAATGCTGT TGGATAGAAG TGGACAAGGG AAAGTGTACC CCCTCATTAA CAGAAGGCGA
    TTTCAGCAGA TGGATGTGCT TGAAGGCTTG AATGTCTTGG TAACTATATC AGGGAAAAAG
    AACAAGCTGA GAGTATATTA CTTATCCTGG CTGAGGAATA AAATTTTGCA CAATGATCCT
    GAAGTAGAGA AGAAACAAGG ATGGACAACA GTTGGGGATT TGGAAGGCTG TGTGCACTAC
    AAAGTTGTAA AATATGAACG TATCAAGTTT TTGGTAATCG CTTTAAAAAG TTCAGTGGAG
    GTGTATGCCT GGGCACCGAA ACCATACCAC AAGTTTATGG CCTTTAAGTC ATTCGGAGAT
    TTAGTACACA GACCTCTCCT TGTAGACCTG ACTGTGGAAG AAGGACAGAG GCTGAAAGTG
    ATCTATGGTT CCTGTGCTGG GTTTCATGCA GTAGATGTGG ACTCGGGCGC AGTTTATGAT
    ATTTACTTGC CAACACATAT TCAGAGCAGC ATTCAGCCAC ACGCAATCAT CATTCTCCCA
    AATACTGATG GTATGGAACT GCTGGTTTGC TATGAAGATG AAGGAGTTTA TGTTAATACA
    TACGGCAGGA TCACCAAAGA TGTAGTTCTA CAATGGGGAG AAATGCCTAC ATCAGTTGCT
    TATATCCGAT CTAACCAGAT CATGGGATGG GGTGAAAAAG CTATTGAGAT TCGATCCGTG
    GAAACTGGCC ACCTTGATGG AGTATTTATG CACAAAAGGG CACAAAGGCT CAAGTTCTTG
    TGCGAACGAA ATGACAAGGT TTTCTTTGCC TCGGTGCGGT CTGGCGGAAG CAGCCAAGTG
    TATTTCATGA CTTTGGGCAG AAGCTCTCTT CTGAGCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35138
    Clone ID Related Accession (Same CDS sequence) XM_012957670.2 , XM_012957670.3
    Accession Version XM_012957670.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3981bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957670.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCCAACG ATTCGCCTGC CAAAAGTCTT GTGGATATAG ACCTGTCGTC TCTGAGGGAC 
    CCAGCTGGCA TCTTTGAATT AGTGGAAGTG GTAGGAAATG GCACCTATGG ACAAGTATAT
    AAGGGCCGAC ATGTCAAAAC AGGCCAACTT GCAGCCATCA AAGTCATGGA TGTTACAGAG
    GATGAAGAGG AAGAAATCAA ACTGGAAATC AATATGTTGA AGAAATATTC CCACCACAGA
    AATATAGCTA CTTATTATGG TGCTTTTATT AAGAAAAGTC CTCCTGGGCA TGATGACCAG
    CTTTGGTTGG TGATGGAGTT TTGTGGTGCT GGCTCAATCA CTGACCTGGT GAAGAATACA
    AAGGGGAATA GTTTGAAAGA AGACTGGATT GCCTATATCT CTCGTGAGAT ACTTAGGGGA
    TTGGCCCACC TCCATGCTCA TCATGTGATT CACCGGGATA TCAAAGGACA GAATGTGCTG
    CTAACAGAGA ATGCCGAAGT GAAATTAGTG GATTTTGGTG TTAGTGCACA GCTGGATAGG
    ACAGTGGGCC GAAGGAACAC ATTTATTGGT ACACCATACT GGATGGCTCC AGAAGTTATC
    GCCTGTGATG AGAACCCAGA TGCTACCTAT GACTATAGGA GTGACCTCTG GTCATGTGGT
    ATTACAGCGA TTGAAATGGC AGAAGGTGCT CCTCCTCTTT GCGATATGCA TCCAATGCGA
    GCCCTGTTCC TTATCCCAAG AAATCCTCCA CCACGGCTAA AATCCAAGAA ATGGTCAAAG
    AAGTTCTTCA GCTTTATAGA AGGCTGCCTT GTGAAAAATT ACATGCAACG TCCCCCAACA
    GAACAGCTTT TAAAGCATCC ATTTATACGA GATCAGCCAA ATGAAAGGCA AGTCCGAATT
    CAGCTGAAGG ATCACATAGA CAGGACAAGG AAGAAGAGAG GAGAAAAAGA TGAAACAGAG
    TATGAATACA GTGGAAGTGA AGAGGAGGAA GAGGAAGTGC CTGAACAGGA AGGAGAACCA
    AGTTCCATTG TTAATGTGCC TGGGGAATCA ACACTCCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGCTC TGAGGCTTTG CGACGGCAAC AGCTGCTGCA GGAGCAACAA
    CTCAGAGAGC AGGAGGAATA TAAAAGACAG CTCCTGGCAG AGAGACAGAA GCGCATAGAA
    CAGCAGAAAG AGCAAAGGAG GAGACTTGAA GAGCAACAAA GAAGAGAGCG TGAGGCTCGT
    CGGCAGCAGG AACGAGAACA AAGAAGGCGA GAGCAAGAGG AAAAGAAGAG AATGGAGGAG
    ATGGAAAGAA GGCGCAAGGA AGAGGAAGAA AGGAGAAGAG CAGAGGAAGA GAAAAGAAGA
    GTGGAAAGAG AGCAGGAGTA TATCCGAAGA CAGCTGGAGG AGGAGCAGCG GCACTTAGAA
    ATCCTTCAGC AGCAGCTGCT TCAGGAGCAG GCCATGTTAC TGGAGTATAG ATGGCGTGAA
    ATGGAGGAGC ACCGCCAGGC TGAAAGACTT CAGCGTCAGT TGCAGCAAGA GCAGGCCTAT
    CTCATGTCAC TACAGCATGA TCATAGACAG CAGCAGCCAC CACAACAGCA GCCCCAGCCC
    CAGCAGCCTC CACTGTCCCA GCAGGAAAGG AGCAAGCAAA CATACCATAC CCAAGAACCC
    AAGCCTCATT ATGAACAAGC AGAACGAATA CGAGAGGCAG AGGAGCGCCT TAGGAAAGCA
    AACCAAGGCT CCCCTGAAGC CCAGGCTAAG CAACCAGGAA GAGTTTTAGA GCCATTAGTG
    CCTTCAAGAT CCGAATCATT TTCCAATGGA AATTCTGAGC CTGCTCAACC TGCCATGCAC
    AGACCAGTGG AACCACAGGT GCCTGTGAGG ACAACATCAA GATCTCCAGT ATTGTCACGG
    AAAGATTCAC CACTGCAGGG CAGTGGGCAG CAAAATACCC AAGCTGGTCA GAGAAATTCC
    ACAAGCAATA TTGAGCCTAG ACTTCTTTGG GAAAGAGTAG AGAAGCTTGT GCCCAGACCG
    GGTAGTGGTA GCTCGTCAGG ATCAAGTAAC TCTGGATCCC AAGCAGGATC CCACCCAGGA
    TCTCAGAGTG GATCTGGAGA GCGCTTCCGG ATGAGATCAT CTTCAAAATC TGAGGGCTCA
    CCATCTCAAC GACATGAAAA CACAGCCAAA AAGTCTGAAG AGAAGAAAGA AGCAGTTAGA
    CAGGCCAAAG CTCCTGGAGA AGTGGATCTT ACTGCACTCG CTAAGGAGCT TCGAGCAGTA
    GATGATGTTC GGCCACTTCA CAAAGTTACT GACTATTCCT CATCCAGTGA AGAGTCTGGT
    ACTACAGATG AGGAGGATGA TGACATGGAA CAGGAAGGAG CAGATGAATC GACTTCAGGC
    CCAGAGGATG CTAGAGCTAT GTCATCTTTG AATTTAAGCA ATGGAGAAAC GGAGTCTGTG
    AAAACCATGA TCGTACATGA TGATGTCGAA AGTGAACCTG CCATGACCCC ATCTAAAGAA
    GGCACTTTAA TTGTCCGACA GAGTACAGTT GACAAAAAGC GTGCCAACCA ACTTGAGAGC
    AATGGCTTTG CGAGTCGTAT TCACCTCTTG CCAGATCTCT TACAGCAAAG CCATTCCTCC
    TCCACCACTT CTTCCCCAAC CTCAAGTCAA CCGACACCTA CCATGTCCCC CCAGACACCC
    CAGGACAAGC TAACTGCTAA TGAGACTCAG TCAGCTAGTA ATACTCTCCA GAAACACAAA
    TCGTCCTCCT CTTTCACACC GTTCATAGAT CCAAGGTTAC TTCAGATCTC TCCATCTAGT
    GGAACAACTG TTACGTCAGT AGGTACATAC ATGCGCACAC TGGAAGCTAA CCTTAAAAAT
    ACTAATTTTA TTTTAGTGGG ATTTTCCAGT GAAGCGCTAA GGCCGGATGC CATGAGGCAA
    GATCCTACAA GGAAAGGATC AGTTGTTAAT GTTAATCCCA CAAATACTCG GCCACAAAGT
    GATACACCAG AGATTCGCAA ATACAAGAAG AGATTTAATT CTGAGATTTT GTGTGCGGCA
    TTGTGGGGTG TAAATTTGCT GGTGGGAACT GAAAGTGGTT TAATGCTGTT GGATAGAAGT
    GGACAAGGGA AAGTGTACCC CCTCATTAAC AGAAGGCGAT TTCAGCAGAT GGATGTGCTT
    GAAGGCTTGA ATGTCTTGGT AACTATATCA GGGAAAAAGA ACAAGCTGAG AGTATATTAC
    TTATCCTGGC TGAGGAATAA AATTTTGCAC AATGATCCTG AAGTAGAGAA GAAACAAGGA
    TGGACAACAG TTGGGGATTT GGAAGGCTGT GTGCACTACA AAGTTGTAAA ATATGAACGT
    ATCAAGTTTT TGGTAATCGC TTTAAAAAGT TCAGTGGAGG TGTATGCCTG GGCACCGAAA
    CCATACCACA AGTTTATGGC CTTTAAGTCA TTCGGAGATT TAGTACACAG ACCTCTCCTT
    GTAGACCTGA CTGTGGAAGA AGGACAGAGG CTGAAAGTGA TCTATGGTTC CTGTGCTGGG
    TTTCATGCAG TAGATGTGGA CTCGGGCGCA GTTTATGATA TTTACTTGCC AACACATATT
    CAGAGCAGCA TTCAGCCACA CGCAATCATC ATTCTCCCAA ATACTGATGG TATGGAACTG
    CTGGTTTGCT ATGAAGATGA AGGAGTTTAT GTTAATACAT ACGGCAGGAT CACCAAAGAT
    GTAGTTCTAC AATGGGGAGA AATGCCTACA TCAGTTGCTT ATATCCGATC TAACCAGATC
    ATGGGATGGG GTGAAAAAGC TATTGAGATT CGATCCGTGG AAACTGGCCA CCTTGATGGA
    GTATTTATGC ACAAAAGGGC ACAAAGGCTC AAGTTCTTGT GCGAACGAAA TGACAAGGTT
    TTCTTTGCCT CGGTGCGGTC TGGCGGAAGC AGCCAAGTGT ATTTCATGAC TTTGGGCAGA
    AGCTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813124.1
    CDS348..4328
    Translation

    Target ORF information:

    RefSeq Version XM_012957670.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 4 (map4k4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957670.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCCAACG ATTCGCCTGC CAAAAGTCTT GTGGATATAG ACCTGTCGTC TCTGAGGGAC 
    CCAGCTGGCA TCTTTGAATT AGTGGAAGTG GTAGGAAATG GCACCTATGG ACAAGTATAT
    AAGGGCCGAC ATGTCAAAAC AGGCCAACTT GCAGCCATCA AAGTCATGGA TGTTACAGAG
    GATGAAGAGG AAGAAATCAA ACTGGAAATC AATATGTTGA AGAAATATTC CCACCACAGA
    AATATAGCTA CTTATTATGG TGCTTTTATT AAGAAAAGTC CTCCTGGGCA TGATGACCAG
    CTTTGGTTGG TGATGGAGTT TTGTGGTGCT GGCTCAATCA CTGACCTGGT GAAGAATACA
    AAGGGGAATA GTTTGAAAGA AGACTGGATT GCCTATATCT CTCGTGAGAT ACTTAGGGGA
    TTGGCCCACC TCCATGCTCA TCATGTGATT CACCGGGATA TCAAAGGACA GAATGTGCTG
    CTAACAGAGA ATGCCGAAGT GAAATTAGTG GATTTTGGTG TTAGTGCACA GCTGGATAGG
    ACAGTGGGCC GAAGGAACAC ATTTATTGGT ACACCATACT GGATGGCTCC AGAAGTTATC
    GCCTGTGATG AGAACCCAGA TGCTACCTAT GACTATAGGA GTGACCTCTG GTCATGTGGT
    ATTACAGCGA TTGAAATGGC AGAAGGTGCT CCTCCTCTTT GCGATATGCA TCCAATGCGA
    GCCCTGTTCC TTATCCCAAG AAATCCTCCA CCACGGCTAA AATCCAAGAA ATGGTCAAAG
    AAGTTCTTCA GCTTTATAGA AGGCTGCCTT GTGAAAAATT ACATGCAACG TCCCCCAACA
    GAACAGCTTT TAAAGCATCC ATTTATACGA GATCAGCCAA ATGAAAGGCA AGTCCGAATT
    CAGCTGAAGG ATCACATAGA CAGGACAAGG AAGAAGAGAG GAGAAAAAGA TGAAACAGAG
    TATGAATACA GTGGAAGTGA AGAGGAGGAA GAGGAAGTGC CTGAACAGGA AGGAGAACCA
    AGTTCCATTG TTAATGTGCC TGGGGAATCA ACACTCCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGCTC TGAGGCTTTG CGACGGCAAC AGCTGCTGCA GGAGCAACAA
    CTCAGAGAGC AGGAGGAATA TAAAAGACAG CTCCTGGCAG AGAGACAGAA GCGCATAGAA
    CAGCAGAAAG AGCAAAGGAG GAGACTTGAA GAGCAACAAA GAAGAGAGCG TGAGGCTCGT
    CGGCAGCAGG AACGAGAACA AAGAAGGCGA GAGCAAGAGG AAAAGAAGAG AATGGAGGAG
    ATGGAAAGAA GGCGCAAGGA AGAGGAAGAA AGGAGAAGAG CAGAGGAAGA GAAAAGAAGA
    GTGGAAAGAG AGCAGGAGTA TATCCGAAGA CAGCTGGAGG AGGAGCAGCG GCACTTAGAA
    ATCCTTCAGC AGCAGCTGCT TCAGGAGCAG GCCATGTTAC TGGAGTATAG ATGGCGTGAA
    ATGGAGGAGC ACCGCCAGGC TGAAAGACTT CAGCGTCAGT TGCAGCAAGA GCAGGCCTAT
    CTCATGTCAC TACAGCATGA TCATAGACAG CAGCAGCCAC CACAACAGCA GCCCCAGCCC
    CAGCAGCCTC CACTGTCCCA GCAGGAAAGG AGCAAGCAAA CATACCATAC CCAAGAACCC
    AAGCCTCATT ATGAACAAGC AGAACGAATA CGAGAGGCAG AGGAGCGCCT TAGGAAAGCA
    AACCAAGGCT CCCCTGAAGC CCAGGCTAAG CAACCAGGAA GAGTTTTAGA GCCATTAGTG
    CCTTCAAGAT CCGAATCATT TTCCAATGGA AATTCTGAGC CTGCTCAACC TGCCATGCAC
    AGACCAGTGG AACCACAGGT GCCTGTGAGG ACAACATCAA GATCTCCAGT ATTGTCACGG
    AAAGATTCAC CACTGCAGGG CAGTGGGCAG CAAAATACCC AAGCTGGTCA GAGAAATTCC
    ACAAGCAATA TTGAGCCTAG ACTTCTTTGG GAAAGAGTAG AGAAGCTTGT GCCCAGACCG
    GGTAGTGGTA GCTCGTCAGG ATCAAGTAAC TCTGGATCCC AAGCAGGATC CCACCCAGGA
    TCTCAGAGTG GATCTGGAGA GCGCTTCCGG ATGAGATCAT CTTCAAAATC TGAGGGCTCA
    CCATCTCAAC GACATGAAAA CACAGCCAAA AAGTCTGAAG AGAAGAAAGA AGCAGTTAGA
    CAGGCCAAAG CTCCTGGAGA AGTGGATCTT ACTGCACTCG CTAAGGAGCT TCGAGCAGTA
    GATGATGTTC GGCCACTTCA CAAAGTTACT GACTATTCCT CATCCAGTGA AGAGTCTGGT
    ACTACAGATG AGGAGGATGA TGACATGGAA CAGGAAGGAG CAGATGAATC GACTTCAGGC
    CCAGAGGATG CTAGAGCTAT GTCATCTTTG AATTTAAGCA ATGGAGAAAC GGAGTCTGTG
    AAAACCATGA TCGTACATGA TGATGTCGAA AGTGAACCTG CCATGACCCC ATCTAAAGAA
    GGCACTTTAA TTGTCCGACA GAGTACAGTT GACAAAAAGC GTGCCAACCA ACTTGAGAGC
    AATGGCTTTG CGAGTCGTAT TCACCTCTTG CCAGATCTCT TACAGCAAAG CCATTCCTCC
    TCCACCACTT CTTCCCCAAC CTCAAGTCAA CCGACACCTA CCATGTCCCC CCAGACACCC
    CAGGACAAGC TAACTGCTAA TGAGACTCAG TCAGCTAGTA ATACTCTCCA GAAACACAAA
    TCGTCCTCCT CTTTCACACC GTTCATAGAT CCAAGGTTAC TTCAGATCTC TCCATCTAGT
    GGAACAACTG TTACGTCAGT AGGTACATAC ATGCGCACAC TGGAAGCTAA CCTTAAAAAT
    ACTAATTTTA TTTTAGTGGG ATTTTCCAGT GAAGCGCTAA GGCCGGATGC CATGAGGCAA
    GATCCTACAA GGAAAGGATC AGTTGTTAAT GTTAATCCCA CAAATACTCG GCCACAAAGT
    GATACACCAG AGATTCGCAA ATACAAGAAG AGATTTAATT CTGAGATTTT GTGTGCGGCA
    TTGTGGGGTG TAAATTTGCT GGTGGGAACT GAAAGTGGTT TAATGCTGTT GGATAGAAGT
    GGACAAGGGA AAGTGTACCC CCTCATTAAC AGAAGGCGAT TTCAGCAGAT GGATGTGCTT
    GAAGGCTTGA ATGTCTTGGT AACTATATCA GGGAAAAAGA ACAAGCTGAG AGTATATTAC
    TTATCCTGGC TGAGGAATAA AATTTTGCAC AATGATCCTG AAGTAGAGAA GAAACAAGGA
    TGGACAACAG TTGGGGATTT GGAAGGCTGT GTGCACTACA AAGTTGTAAA ATATGAACGT
    ATCAAGTTTT TGGTAATCGC TTTAAAAAGT TCAGTGGAGG TGTATGCCTG GGCACCGAAA
    CCATACCACA AGTTTATGGC CTTTAAGTCA TTCGGAGATT TAGTACACAG ACCTCTCCTT
    GTAGACCTGA CTGTGGAAGA AGGACAGAGG CTGAAAGTGA TCTATGGTTC CTGTGCTGGG
    TTTCATGCAG TAGATGTGGA CTCGGGCGCA GTTTATGATA TTTACTTGCC AACACATATT
    CAGAGCAGCA TTCAGCCACA CGCAATCATC ATTCTCCCAA ATACTGATGG TATGGAACTG
    CTGGTTTGCT ATGAAGATGA AGGAGTTTAT GTTAATACAT ACGGCAGGAT CACCAAAGAT
    GTAGTTCTAC AATGGGGAGA AATGCCTACA TCAGTTGCTT ATATCCGATC TAACCAGATC
    ATGGGATGGG GTGAAAAAGC TATTGAGATT CGATCCGTGG AAACTGGCCA CCTTGATGGA
    GTATTTATGC ACAAAAGGGC ACAAAGGCTC AAGTTCTTGT GCGAACGAAA TGACAAGGTT
    TTCTTTGCCT CGGTGCGGTC TGGCGGAAGC AGCCAAGTGT ATTTCATGAC TTTGGGCAGA
    AGCTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35139
    Clone ID Related Accession (Same CDS sequence) XM_012957671.2 , XM_012957671.3
    Accession Version XM_012957671.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3975bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957671.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCCAACG ATTCGCCTGC CAAAAGTCTT GTGGATATAG ACCTGTCGTC TCTGAGGGAC 
    CCAGCTGGCA TCTTTGAATT AGTGGAAGTG GTAGGAAATG GCACCTATGG ACAAGTATAT
    AAGGGCCGAC ATGTCAAAAC AGGCCAACTT GCAGCCATCA AAGTCATGGA TGTTACAGAG
    GATGAAGAGG AAGAAATCAA ACTGGAAATC AATATGTTGA AGAAATATTC CCACCACAGA
    AATATAGCTA CTTATTATGG TGCTTTTATT AAGAAAAGTC CTCCTGGGCA TGATGACCAG
    CTTTGGTTGG TGATGGAGTT TTGTGGTGCT GGCTCAATCA CTGACCTGGT GAAGAATACA
    AAGGGGAATA GTTTGAAAGA AGACTGGATT GCCTATATCT CTCGTGAGAT ACTTAGGGGA
    TTGGCCCACC TCCATGCTCA TCATGTGATT CACCGGGATA TCAAAGGACA GAATGTGCTG
    CTAACAGAGA ATGCCGAAGT GAAATTAGTG GATTTTGGTG TTAGTGCACA GCTGGATAGG
    ACAGTGGGCC GAAGGAACAC ATTTATTGGT ACACCATACT GGATGGCTCC AGAAGTTATC
    GCCTGTGATG AGAACCCAGA TGCTACCTAT GACTATAGGA GTGACCTCTG GTCATGTGGT
    ATTACAGCGA TTGAAATGGC AGAAGGTGCT CCTCCTCTTT GCGATATGCA TCCAATGCGA
    GCCCTGTTCC TTATCCCAAG AAATCCTCCA CCACGGCTAA AATCCAAGAA ATGGTCAAAG
    AAGTTCTTCA GCTTTATAGA AGGCTGCCTT GTGAAAAATT ACATGCAACG TCCCCCAACA
    GAACAGCTTT TAAAGCATCC ATTTATACGA GATCAGCCAA ATGAAAGGCA AGTCCGAATT
    CAGCTGAAGG ATCACATAGA CAGGACAAGG AAGAAGAGAG GAGAAAAAGA TGAAACAGAG
    TATGAATACA GTGGAAGTGA AGAGGAGGAA GAGGAAGTGC CTGAACAGGA AGGAGAACCA
    AGTTCCATTG TTAATGTGCC TGGGGAATCA ACACTCCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGCTC TGAGGCTTTG CGACGGCAAC AGCTGCTGCA GGAGCAACAA
    CTCAGAGAGC AGGAGGAATA TAAAAGACAG CTCCTGGCAG AGAGACAGAA GCGCATAGAA
    CAGCAGAAAG AGCAAAGGAG GAGACTTGAA GAGCAACAAA GAAGAGAGCG TGAGGCTCGT
    CGGCAGCAGG AACGAGAACA AAGAAGGCGA GAGCAAGAGG AAAAGAAGAG AATGGAGGAG
    ATGGAAAGAA GGCGCAAGGA AGAGGAAGAA AGGAGAAGAG CAGAGGAAGA GAAAAGAAGA
    GTGGAAAGAG AGCAGGAGTA TATCCGAAGA CAGCTGGAGG AGGAGCAGCG GCACTTAGAA
    ATCCTTCAGC AGCAGCTGCT TCAGGAGCAG GCCATGTTAC TGGAGTATAG ATGGCGTGAA
    ATGGAGGAGC ACCGCCAGGC TGAAAGACTT CAGCGTCAGT TGCAGCAAGA GCAGGCCTAT
    CTCATGTCAC TACAGCATGA TCATAGACAG CAGCAGCCAC CACAACAGCA GCCCCAGCCC
    CAGCAGCCTC CACTGTCCCA GCAGGAAAGG AGCAAGCAAA CATACCATAC CCAAGAACCC
    AAGCCTCATT ATGAACAAGC AGAACGAATA CGAGAGGCAG AGGAGCGCCT TAGGAAAGCA
    AACCAAGGCT CCCCTGAAGC CCAGGCTAAG CAACCAGGAA GAGTTTTAGA GCCATTAGTG
    CCTTCAAGAT CCGAATCATT TTCCAATGGA AATTCTGAGC CTGCTCAACC TGCCATGCAC
    AGACCAGTGG AACCACAGGT ACAGTGGTCT CATCTGGCAT CCCTCAAGAA CAATGTATCC
    CCTGTCTCGC GATCCCATTC CTTCAGTGAC CCTTCTCCCA AATTTGCACC TCACCTTCTA
    CGATCTCAGG ATTCGCACCA AACTTCACGC AGTGAAGTGC AAAGTCAGGG TTCTGACCCT
    AAGTCTGAAA TCTCTGAGCC AACCCAAAGG GTTTGGGCTC GATCAGACAG TGACGAGGTG
    CCTCCAAGGG TGCCTGTGAG GACAACATCA AGATCTCCAG TATTGTCACG GAAAGATTCA
    CCACTGCAGG GCAGTGGGCA GCAAAATACC CAAGCTGGTC AGAGAAATTC CACAAGCAAT
    ATTGAGCCTA GACTTCTTTG GGAAAGAGTA GAGAAGCTTG TGCCCAGACC GGGTAGTGGT
    AGCTCGTCAG GATCAAGTAA CTCTGGATCC CAAGCAGGAT CCCACCCAGG ATCTCAGAGT
    GGATCTGGAG AGCGCTTCCG GATGAGATCA TCTTCAAAAT CTGAGGGCTC ACCATCTCAA
    CGACATGAAA ACACAGCCAA AAAGTCTGAA GAGAAGAAAG AAGCAGTTAG ACAGGCCAAA
    GCTCCTGGAG AAGTGGATCT TACTGCACTC GCTAAGGAGC TTCGAGCAGT AGATGATGTT
    CGGCCACTTC ACAAAGTTAC TGACTATTCC TCATCCAGTG AAGAGTCTGG TACTACAGAT
    GAGGAGGATG ATGACATGGA ACAGGAAGGA GCAGATGAAT CGACTTCAGG CCCAGAGGAT
    GCTAGAGCTA TGTCATCTTT GAATTTAAGC AATGGAGAAA CGGAGTCTGT GAAAACCATG
    ATCGTACATG ATGATGTCGA AAGTGAACCT GCCATGACCC CATCTAAAGA AGGCACTTTA
    ATTGTCCGAC AGACTCAGTC AGCTAGTAAT ACTCTCCAGA AACACAAATC GTCCTCCTCT
    TTCACACCGT TCATAGATCC AAGGTTACTT CAGATCTCTC CATCTAGTGG AACAACTGTT
    ACGTCAGTAG TGGGATTTTC CAGTGAAGCG CTAAGGCCGG ATGCCATGAG GCAAGATCCT
    ACAAGGAAAG GATCAGTTGT TAATGTTAAT CCCACAAATA CTCGGCCACA AAGTGATACA
    CCAGAGATTC GCAAATACAA GAAGAGATTT AATTCTGAGA TTTTGTGTGC GGCATTGTGG
    GGTGTAAATT TGCTGGTGGG AACTGAAAGT GGTTTAATGC TGTTGGATAG AAGTGGACAA
    GGGAAAGTGT ACCCCCTCAT TAACAGAAGG CGATTTCAGC AGATGGATGT GCTTGAAGGC
    TTGAATGTCT TGGTAACTAT ATCAGGGAAA AAGAACAAGC TGAGAGTATA TTACTTATCC
    TGGCTGAGGA ATAAAATTTT GCACAATGAT CCTGAAGTAG AGAAGAAACA AGGATGGACA
    ACAGTTGGGG ATTTGGAAGG CTGTGTGCAC TACAAAGTTG TAAAATATGA ACGTATCAAG
    TTTTTGGTAA TCGCTTTAAA AAGTTCAGTG GAGGTGTATG CCTGGGCACC GAAACCATAC
    CACAAGTTTA TGGCCTTTAA GTCATTCGGA GATTTAGTAC ACAGACCTCT CCTTGTAGAC
    CTGACTGTGG AAGAAGGACA GAGGCTGAAA GTGATCTATG GTTCCTGTGC TGGGTTTCAT
    GCAGTAGATG TGGACTCGGG CGCAGTTTAT GATATTTACT TGCCAACACA TATTCAGAGC
    AGCATTCAGC CACACGCAAT CATCATTCTC CCAAATACTG ATGGTATGGA ACTGCTGGTT
    TGCTATGAAG ATGAAGGAGT TTATGTTAAT ACATACGGCA GGATCACCAA AGATGTAGTT
    CTACAATGGG GAGAAATGCC TACATCAGTT GCTTATATCC GATCTAACCA GATCATGGGA
    TGGGGTGAAA AAGCTATTGA GATTCGATCC GTGGAAACTG GCCACCTTGA TGGAGTATTT
    ATGCACAAAA GGGCACAAAG GCTCAAGTTC TTGTGCGAAC GAAATGACAA GGTTTTCTTT
    GCCTCGGTGC GGTCTGGCGG AAGCAGCCAA GTGTATTTCA TGACTTTGGG CAGAAGCTCT
    CTTCTGAGCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813125.1
    CDS347..4321
    Translation

    Target ORF information:

    RefSeq Version XM_012957671.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 4 (map4k4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957671.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCCAACG ATTCGCCTGC CAAAAGTCTT GTGGATATAG ACCTGTCGTC TCTGAGGGAC 
    CCAGCTGGCA TCTTTGAATT AGTGGAAGTG GTAGGAAATG GCACCTATGG ACAAGTATAT
    AAGGGCCGAC ATGTCAAAAC AGGCCAACTT GCAGCCATCA AAGTCATGGA TGTTACAGAG
    GATGAAGAGG AAGAAATCAA ACTGGAAATC AATATGTTGA AGAAATATTC CCACCACAGA
    AATATAGCTA CTTATTATGG TGCTTTTATT AAGAAAAGTC CTCCTGGGCA TGATGACCAG
    CTTTGGTTGG TGATGGAGTT TTGTGGTGCT GGCTCAATCA CTGACCTGGT GAAGAATACA
    AAGGGGAATA GTTTGAAAGA AGACTGGATT GCCTATATCT CTCGTGAGAT ACTTAGGGGA
    TTGGCCCACC TCCATGCTCA TCATGTGATT CACCGGGATA TCAAAGGACA GAATGTGCTG
    CTAACAGAGA ATGCCGAAGT GAAATTAGTG GATTTTGGTG TTAGTGCACA GCTGGATAGG
    ACAGTGGGCC GAAGGAACAC ATTTATTGGT ACACCATACT GGATGGCTCC AGAAGTTATC
    GCCTGTGATG AGAACCCAGA TGCTACCTAT GACTATAGGA GTGACCTCTG GTCATGTGGT
    ATTACAGCGA TTGAAATGGC AGAAGGTGCT CCTCCTCTTT GCGATATGCA TCCAATGCGA
    GCCCTGTTCC TTATCCCAAG AAATCCTCCA CCACGGCTAA AATCCAAGAA ATGGTCAAAG
    AAGTTCTTCA GCTTTATAGA AGGCTGCCTT GTGAAAAATT ACATGCAACG TCCCCCAACA
    GAACAGCTTT TAAAGCATCC ATTTATACGA GATCAGCCAA ATGAAAGGCA AGTCCGAATT
    CAGCTGAAGG ATCACATAGA CAGGACAAGG AAGAAGAGAG GAGAAAAAGA TGAAACAGAG
    TATGAATACA GTGGAAGTGA AGAGGAGGAA GAGGAAGTGC CTGAACAGGA AGGAGAACCA
    AGTTCCATTG TTAATGTGCC TGGGGAATCA ACACTCCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGCTC TGAGGCTTTG CGACGGCAAC AGCTGCTGCA GGAGCAACAA
    CTCAGAGAGC AGGAGGAATA TAAAAGACAG CTCCTGGCAG AGAGACAGAA GCGCATAGAA
    CAGCAGAAAG AGCAAAGGAG GAGACTTGAA GAGCAACAAA GAAGAGAGCG TGAGGCTCGT
    CGGCAGCAGG AACGAGAACA AAGAAGGCGA GAGCAAGAGG AAAAGAAGAG AATGGAGGAG
    ATGGAAAGAA GGCGCAAGGA AGAGGAAGAA AGGAGAAGAG CAGAGGAAGA GAAAAGAAGA
    GTGGAAAGAG AGCAGGAGTA TATCCGAAGA CAGCTGGAGG AGGAGCAGCG GCACTTAGAA
    ATCCTTCAGC AGCAGCTGCT TCAGGAGCAG GCCATGTTAC TGGAGTATAG ATGGCGTGAA
    ATGGAGGAGC ACCGCCAGGC TGAAAGACTT CAGCGTCAGT TGCAGCAAGA GCAGGCCTAT
    CTCATGTCAC TACAGCATGA TCATAGACAG CAGCAGCCAC CACAACAGCA GCCCCAGCCC
    CAGCAGCCTC CACTGTCCCA GCAGGAAAGG AGCAAGCAAA CATACCATAC CCAAGAACCC
    AAGCCTCATT ATGAACAAGC AGAACGAATA CGAGAGGCAG AGGAGCGCCT TAGGAAAGCA
    AACCAAGGCT CCCCTGAAGC CCAGGCTAAG CAACCAGGAA GAGTTTTAGA GCCATTAGTG
    CCTTCAAGAT CCGAATCATT TTCCAATGGA AATTCTGAGC CTGCTCAACC TGCCATGCAC
    AGACCAGTGG AACCACAGGT ACAGTGGTCT CATCTGGCAT CCCTCAAGAA CAATGTATCC
    CCTGTCTCGC GATCCCATTC CTTCAGTGAC CCTTCTCCCA AATTTGCACC TCACCTTCTA
    CGATCTCAGG ATTCGCACCA AACTTCACGC AGTGAAGTGC AAAGTCAGGG TTCTGACCCT
    AAGTCTGAAA TCTCTGAGCC AACCCAAAGG GTTTGGGCTC GATCAGACAG TGACGAGGTG
    CCTCCAAGGG TGCCTGTGAG GACAACATCA AGATCTCCAG TATTGTCACG GAAAGATTCA
    CCACTGCAGG GCAGTGGGCA GCAAAATACC CAAGCTGGTC AGAGAAATTC CACAAGCAAT
    ATTGAGCCTA GACTTCTTTG GGAAAGAGTA GAGAAGCTTG TGCCCAGACC GGGTAGTGGT
    AGCTCGTCAG GATCAAGTAA CTCTGGATCC CAAGCAGGAT CCCACCCAGG ATCTCAGAGT
    GGATCTGGAG AGCGCTTCCG GATGAGATCA TCTTCAAAAT CTGAGGGCTC ACCATCTCAA
    CGACATGAAA ACACAGCCAA AAAGTCTGAA GAGAAGAAAG AAGCAGTTAG ACAGGCCAAA
    GCTCCTGGAG AAGTGGATCT TACTGCACTC GCTAAGGAGC TTCGAGCAGT AGATGATGTT
    CGGCCACTTC ACAAAGTTAC TGACTATTCC TCATCCAGTG AAGAGTCTGG TACTACAGAT
    GAGGAGGATG ATGACATGGA ACAGGAAGGA GCAGATGAAT CGACTTCAGG CCCAGAGGAT
    GCTAGAGCTA TGTCATCTTT GAATTTAAGC AATGGAGAAA CGGAGTCTGT GAAAACCATG
    ATCGTACATG ATGATGTCGA AAGTGAACCT GCCATGACCC CATCTAAAGA AGGCACTTTA
    ATTGTCCGAC AGACTCAGTC AGCTAGTAAT ACTCTCCAGA AACACAAATC GTCCTCCTCT
    TTCACACCGT TCATAGATCC AAGGTTACTT CAGATCTCTC CATCTAGTGG AACAACTGTT
    ACGTCAGTAG TGGGATTTTC CAGTGAAGCG CTAAGGCCGG ATGCCATGAG GCAAGATCCT
    ACAAGGAAAG GATCAGTTGT TAATGTTAAT CCCACAAATA CTCGGCCACA AAGTGATACA
    CCAGAGATTC GCAAATACAA GAAGAGATTT AATTCTGAGA TTTTGTGTGC GGCATTGTGG
    GGTGTAAATT TGCTGGTGGG AACTGAAAGT GGTTTAATGC TGTTGGATAG AAGTGGACAA
    GGGAAAGTGT ACCCCCTCAT TAACAGAAGG CGATTTCAGC AGATGGATGT GCTTGAAGGC
    TTGAATGTCT TGGTAACTAT ATCAGGGAAA AAGAACAAGC TGAGAGTATA TTACTTATCC
    TGGCTGAGGA ATAAAATTTT GCACAATGAT CCTGAAGTAG AGAAGAAACA AGGATGGACA
    ACAGTTGGGG ATTTGGAAGG CTGTGTGCAC TACAAAGTTG TAAAATATGA ACGTATCAAG
    TTTTTGGTAA TCGCTTTAAA AAGTTCAGTG GAGGTGTATG CCTGGGCACC GAAACCATAC
    CACAAGTTTA TGGCCTTTAA GTCATTCGGA GATTTAGTAC ACAGACCTCT CCTTGTAGAC
    CTGACTGTGG AAGAAGGACA GAGGCTGAAA GTGATCTATG GTTCCTGTGC TGGGTTTCAT
    GCAGTAGATG TGGACTCGGG CGCAGTTTAT GATATTTACT TGCCAACACA TATTCAGAGC
    AGCATTCAGC CACACGCAAT CATCATTCTC CCAAATACTG ATGGTATGGA ACTGCTGGTT
    TGCTATGAAG ATGAAGGAGT TTATGTTAAT ACATACGGCA GGATCACCAA AGATGTAGTT
    CTACAATGGG GAGAAATGCC TACATCAGTT GCTTATATCC GATCTAACCA GATCATGGGA
    TGGGGTGAAA AAGCTATTGA GATTCGATCC GTGGAAACTG GCCACCTTGA TGGAGTATTT
    ATGCACAAAA GGGCACAAAG GCTCAAGTTC TTGTGCGAAC GAAATGACAA GGTTTTCTTT
    GCCTCGGTGC GGTCTGGCGG AAGCAGCCAA GTGTATTTCA TGACTTTGGG CAGAAGCTCT
    CTTCTGAGCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35140
    Clone ID Related Accession (Same CDS sequence) XM_012957672.3 , XM_012957672.2
    Accession Version XM_012957672.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3744bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957672.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCCAACG ATTCGCCTGC CAAAAGTCTT GTGGATATAG ACCTGTCGTC TCTGAGGGAC 
    CCAGCTGGCA TCTTTGAATT AGTGGAAGTG GTAGGAAATG GCACCTATGG ACAAGTATAT
    AAGGGCCGAC ATGTCAAAAC AGGCCAACTT GCAGCCATCA AAGTCATGGA TGTTACAGAG
    GATGAAGAGG AAGAAATCAA ACTGGAAATC AATATGTTGA AGAAATATTC CCACCACAGA
    AATATAGCTA CTTATTATGG TGCTTTTATT AAGAAAAGTC CTCCTGGGCA TGATGACCAG
    CTTTGGTTGG TGATGGAGTT TTGTGGTGCT GGCTCAATCA CTGACCTGGT GAAGAATACA
    AAGGGGAATA GTTTGAAAGA AGACTGGATT GCCTATATCT CTCGTGAGAT ACTTAGGGGA
    TTGGCCCACC TCCATGCTCA TCATGTGATT CACCGGGATA TCAAAGGACA GAATGTGCTG
    CTAACAGAGA ATGCCGAAGT GAAATTAGTG GATTTTGGTG TTAGTGCACA GCTGGATAGG
    ACAGTGGGCC GAAGGAACAC ATTTATTGGT ACACCATACT GGATGGCTCC AGAAGTTATC
    GCCTGTGATG AGAACCCAGA TGCTACCTAT GACTATAGGA GTGACCTCTG GTCATGTGGT
    ATTACAGCGA TTGAAATGGC AGAAGGTGCT CCTCCTCTTT GCGATATGCA TCCAATGCGA
    GCCCTGTTCC TTATCCCAAG AAATCCTCCA CCACGGCTAA AATCCAAGAA ATGGTCAAAG
    AAGTTCTTCA GCTTTATAGA AGGCTGCCTT GTGAAAAATT ACATGCAACG TCCCCCAACA
    GAACAGCTTT TAAAGCATCC ATTTATACGA GATCAGCCAA ATGAAAGGCA AGTCCGAATT
    CAGCTGAAGG ATCACATAGA CAGGACAAGG AAGAAGAGAG GAGAAAAAGA TGAAACAGAG
    TATGAATACA GTGGAAGTGA AGAGGAGGAA GAGGAAGTGC CTGAACAGGA AGGAGAACCA
    AGTTCCATTG TTAATGTGCC TGGGGAATCA ACACTCCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGCTC TGAGGCTTTG CGACGGCAAC AGCTGCTGCA GGAGCAACAA
    CTCAGAGAGC AGGAGGAATA TAAAAGACAG CTCCTGGCAG AGAGACAGAA GCGCATAGAA
    CAGCAGAAAG AGCAAAGGAG GAGACTTGAA GAGCAACAAA GAAGAGAGCG TGAGGCTCGT
    CGGCAGCAGG AACGAGAACA AAGAAGGCGA GAGCAAGAGG AAAAGAAGAG AATGGAGGAG
    ATGGAAAGAA GGCGCAAGGA AGAGGAAGAA AGGAGAAGAG CAGAGGAAGA GAAAAGAAGA
    GTGGAAAGAG AGCAGGAGTA TATCCGAAGA CAGCTGGAGG AGGAGCAGCG GCACTTAGAA
    ATCCTTCAGC AGCAGCTGCT TCAGGAGCAG GCCATGTTAC TGGAGTATAG ATGGCGTGAA
    ATGGAGGAGC ACCGCCAGGC TGAAAGACTT CAGCGTCAGT TGCAGCAAGA GCAGGCCTAT
    CTCATGTCAC TACAGCATGA TCATAGACAG CAGCAGCCAC CACAACAGCA GCCCCAGCCC
    CAGCAGCCTC CACTGTCCCA GCAGGAAAGG AGCAAGCAAA CATACCATAC CCAAGAACCC
    AAGCCTCATT ATGAACAAGC AGAACGAATA CGAGAGGCAG AGGAGCGCCT TAGGAAAGCA
    AACCAAGGCT CCCCTGAAGC CCAGGCTAAG CAACCAGGAA GAGTTTTAGA GCCATTAGTG
    CCTTCAAGAT CCGAATCATT TTCCAATGGA AATTCTGAGC CTGCTCAACC TGCCATGCAC
    AGACCAGTGG AACCACAGGT GCCTGTGAGG ACAACATCAA GATCTCCAGT ATTGTCACGG
    AAAGATTCAC CACTGCAGGG CAGTGGGCAG CAAAATACCC AAGCTGGTCA GAGAAATTCC
    ACAAGCAATA TTGAGCCTAG ACTTCTTTGG GAAAGAGTAG AGAAGCTTGT GCCCAGACCG
    GGTAGTGGTA GCTCGTCAGG ATCAAGTAAC TCTGGATCCC AAGCAGGATC CCACCCAGGA
    TCTCAGAGTG GATCTGGAGA GCGCTTCCGG ATGAGATCAT CTTCAAAATC TGAGGGCTCA
    CCATCTCAAC GACATGAAAA CACAGCCAAA AAGTCTGAAG AGAAGAAAGA AGCAGTTAGA
    CAGGCCAAAG CTCCTGGAGA AGTGGATCTT ACTGCACTCG CTAAGGAGCT TCGAGCAGTA
    GATGATGTTC GGCCACTTCA CAAAGTTACT GACTATTCCT CATCCAGTGA AGAGTCTGGT
    ACTACAGATG AGGAGGATGA TGACATGGAA CAGGAAGGAG CAGATGAATC GACTTCAGGC
    CCAGAGGATG CTAGAGCTAT GTCATCTTTG AATTTAAGCA ATGGAGAAAC GGAGTCTGTG
    AAAACCATGA TCGTACATGA TGATGTCGAA AGTGAACCTG CCATGACCCC ATCTAAAGAA
    GGCACTTTAA TTGTCCGACA GACTCAGTCA GCTAGTAATA CTCTCCAGAA ACACAAATCG
    TCCTCCTCTT TCACACCGTT CATAGATCCA AGGTTACTTC AGATCTCTCC ATCTAGTGGA
    ACAACTGTTA CGTCAGTAGT GGGATTTTCC AGTGAAGCGC TAAGGCCGGA TGCCATGAGG
    CAAGATCCTA CAAGGAAAGG ATCAGTTGTT AATGTTAATC CCACAAATAC TCGGCCACAA
    AGTGATACAC CAGAGATTCG CAAATACAAG AAGAGATTTA ATTCTGAGAT TTTGTGTGCG
    GCATTGTGGG GTGTAAATTT GCTGGTGGGA ACTGAAAGTG GTTTAATGCT GTTGGATAGA
    AGTGGACAAG GGAAAGTGTA CCCCCTCATT AACAGAAGGC GATTTCAGCA GATGGATGTG
    CTTGAAGGCT TGAATGTCTT GGTAACTATA TCAGGGAAAA AGAACAAGCT GAGAGTATAT
    TACTTATCCT GGCTGAGGAA TAAAATTTTG CACAATGATC CTGAAGTAGA GAAGAAACAA
    GGATGGACAA CAGTTGGGGA TTTGGAAGGC TGTGTGCACT ACAAAGTTGT AAAATATGAA
    CGTATCAAGT TTTTGGTAAT CGCTTTAAAA AGTTCAGTGG AGGTGTATGC CTGGGCACCG
    AAACCATACC ACAAGTTTAT GGCCTTTAAG TCATTCGGAG ATTTAGTACA CAGACCTCTC
    CTTGTAGACC TGACTGTGGA AGAAGGACAG AGGCTGAAAG TGATCTATGG TTCCTGTGCT
    GGGTTTCATG CAGTAGATGT GGACTCGGGC GCAGTTTATG ATATTTACTT GCCAACACAT
    ATTCAGAGCA GCATTCAGCC ACACGCAATC ATCATTCTCC CAAATACTGA TGGTATGGAA
    CTGCTGGTTT GCTATGAAGA TGAAGGAGTT TATGTTAATA CATACGGCAG GATCACCAAA
    GATGTAGTTC TACAATGGGG AGAAATGCCT ACATCAGTTG CTTATATCCG ATCTAACCAG
    ATCATGGGAT GGGGTGAAAA AGCTATTGAG ATTCGATCCG TGGAAACTGG CCACCTTGAT
    GGAGTATTTA TGCACAAAAG GGCACAAAGG CTCAAGTTCT TGTGCGAACG AAATGACAAG
    GTTTTCTTTG CCTCGGTGCG GTCTGGCGGA AGCAGCCAAG TGTATTTCAT GACTTTGGGC
    AGAAGCTCTC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813126.1
    CDS348..4091
    Translation

    Target ORF information:

    RefSeq Version XM_012957672.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 4 (map4k4), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957672.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCCAACG ATTCGCCTGC CAAAAGTCTT GTGGATATAG ACCTGTCGTC TCTGAGGGAC 
    CCAGCTGGCA TCTTTGAATT AGTGGAAGTG GTAGGAAATG GCACCTATGG ACAAGTATAT
    AAGGGCCGAC ATGTCAAAAC AGGCCAACTT GCAGCCATCA AAGTCATGGA TGTTACAGAG
    GATGAAGAGG AAGAAATCAA ACTGGAAATC AATATGTTGA AGAAATATTC CCACCACAGA
    AATATAGCTA CTTATTATGG TGCTTTTATT AAGAAAAGTC CTCCTGGGCA TGATGACCAG
    CTTTGGTTGG TGATGGAGTT TTGTGGTGCT GGCTCAATCA CTGACCTGGT GAAGAATACA
    AAGGGGAATA GTTTGAAAGA AGACTGGATT GCCTATATCT CTCGTGAGAT ACTTAGGGGA
    TTGGCCCACC TCCATGCTCA TCATGTGATT CACCGGGATA TCAAAGGACA GAATGTGCTG
    CTAACAGAGA ATGCCGAAGT GAAATTAGTG GATTTTGGTG TTAGTGCACA GCTGGATAGG
    ACAGTGGGCC GAAGGAACAC ATTTATTGGT ACACCATACT GGATGGCTCC AGAAGTTATC
    GCCTGTGATG AGAACCCAGA TGCTACCTAT GACTATAGGA GTGACCTCTG GTCATGTGGT
    ATTACAGCGA TTGAAATGGC AGAAGGTGCT CCTCCTCTTT GCGATATGCA TCCAATGCGA
    GCCCTGTTCC TTATCCCAAG AAATCCTCCA CCACGGCTAA AATCCAAGAA ATGGTCAAAG
    AAGTTCTTCA GCTTTATAGA AGGCTGCCTT GTGAAAAATT ACATGCAACG TCCCCCAACA
    GAACAGCTTT TAAAGCATCC ATTTATACGA GATCAGCCAA ATGAAAGGCA AGTCCGAATT
    CAGCTGAAGG ATCACATAGA CAGGACAAGG AAGAAGAGAG GAGAAAAAGA TGAAACAGAG
    TATGAATACA GTGGAAGTGA AGAGGAGGAA GAGGAAGTGC CTGAACAGGA AGGAGAACCA
    AGTTCCATTG TTAATGTGCC TGGGGAATCA ACACTCCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGCTC TGAGGCTTTG CGACGGCAAC AGCTGCTGCA GGAGCAACAA
    CTCAGAGAGC AGGAGGAATA TAAAAGACAG CTCCTGGCAG AGAGACAGAA GCGCATAGAA
    CAGCAGAAAG AGCAAAGGAG GAGACTTGAA GAGCAACAAA GAAGAGAGCG TGAGGCTCGT
    CGGCAGCAGG AACGAGAACA AAGAAGGCGA GAGCAAGAGG AAAAGAAGAG AATGGAGGAG
    ATGGAAAGAA GGCGCAAGGA AGAGGAAGAA AGGAGAAGAG CAGAGGAAGA GAAAAGAAGA
    GTGGAAAGAG AGCAGGAGTA TATCCGAAGA CAGCTGGAGG AGGAGCAGCG GCACTTAGAA
    ATCCTTCAGC AGCAGCTGCT TCAGGAGCAG GCCATGTTAC TGGAGTATAG ATGGCGTGAA
    ATGGAGGAGC ACCGCCAGGC TGAAAGACTT CAGCGTCAGT TGCAGCAAGA GCAGGCCTAT
    CTCATGTCAC TACAGCATGA TCATAGACAG CAGCAGCCAC CACAACAGCA GCCCCAGCCC
    CAGCAGCCTC CACTGTCCCA GCAGGAAAGG AGCAAGCAAA CATACCATAC CCAAGAACCC
    AAGCCTCATT ATGAACAAGC AGAACGAATA CGAGAGGCAG AGGAGCGCCT TAGGAAAGCA
    AACCAAGGCT CCCCTGAAGC CCAGGCTAAG CAACCAGGAA GAGTTTTAGA GCCATTAGTG
    CCTTCAAGAT CCGAATCATT TTCCAATGGA AATTCTGAGC CTGCTCAACC TGCCATGCAC
    AGACCAGTGG AACCACAGGT GCCTGTGAGG ACAACATCAA GATCTCCAGT ATTGTCACGG
    AAAGATTCAC CACTGCAGGG CAGTGGGCAG CAAAATACCC AAGCTGGTCA GAGAAATTCC
    ACAAGCAATA TTGAGCCTAG ACTTCTTTGG GAAAGAGTAG AGAAGCTTGT GCCCAGACCG
    GGTAGTGGTA GCTCGTCAGG ATCAAGTAAC TCTGGATCCC AAGCAGGATC CCACCCAGGA
    TCTCAGAGTG GATCTGGAGA GCGCTTCCGG ATGAGATCAT CTTCAAAATC TGAGGGCTCA
    CCATCTCAAC GACATGAAAA CACAGCCAAA AAGTCTGAAG AGAAGAAAGA AGCAGTTAGA
    CAGGCCAAAG CTCCTGGAGA AGTGGATCTT ACTGCACTCG CTAAGGAGCT TCGAGCAGTA
    GATGATGTTC GGCCACTTCA CAAAGTTACT GACTATTCCT CATCCAGTGA AGAGTCTGGT
    ACTACAGATG AGGAGGATGA TGACATGGAA CAGGAAGGAG CAGATGAATC GACTTCAGGC
    CCAGAGGATG CTAGAGCTAT GTCATCTTTG AATTTAAGCA ATGGAGAAAC GGAGTCTGTG
    AAAACCATGA TCGTACATGA TGATGTCGAA AGTGAACCTG CCATGACCCC ATCTAAAGAA
    GGCACTTTAA TTGTCCGACA GACTCAGTCA GCTAGTAATA CTCTCCAGAA ACACAAATCG
    TCCTCCTCTT TCACACCGTT CATAGATCCA AGGTTACTTC AGATCTCTCC ATCTAGTGGA
    ACAACTGTTA CGTCAGTAGT GGGATTTTCC AGTGAAGCGC TAAGGCCGGA TGCCATGAGG
    CAAGATCCTA CAAGGAAAGG ATCAGTTGTT AATGTTAATC CCACAAATAC TCGGCCACAA
    AGTGATACAC CAGAGATTCG CAAATACAAG AAGAGATTTA ATTCTGAGAT TTTGTGTGCG
    GCATTGTGGG GTGTAAATTT GCTGGTGGGA ACTGAAAGTG GTTTAATGCT GTTGGATAGA
    AGTGGACAAG GGAAAGTGTA CCCCCTCATT AACAGAAGGC GATTTCAGCA GATGGATGTG
    CTTGAAGGCT TGAATGTCTT GGTAACTATA TCAGGGAAAA AGAACAAGCT GAGAGTATAT
    TACTTATCCT GGCTGAGGAA TAAAATTTTG CACAATGATC CTGAAGTAGA GAAGAAACAA
    GGATGGACAA CAGTTGGGGA TTTGGAAGGC TGTGTGCACT ACAAAGTTGT AAAATATGAA
    CGTATCAAGT TTTTGGTAAT CGCTTTAAAA AGTTCAGTGG AGGTGTATGC CTGGGCACCG
    AAACCATACC ACAAGTTTAT GGCCTTTAAG TCATTCGGAG ATTTAGTACA CAGACCTCTC
    CTTGTAGACC TGACTGTGGA AGAAGGACAG AGGCTGAAAG TGATCTATGG TTCCTGTGCT
    GGGTTTCATG CAGTAGATGT GGACTCGGGC GCAGTTTATG ATATTTACTT GCCAACACAT
    ATTCAGAGCA GCATTCAGCC ACACGCAATC ATCATTCTCC CAAATACTGA TGGTATGGAA
    CTGCTGGTTT GCTATGAAGA TGAAGGAGTT TATGTTAATA CATACGGCAG GATCACCAAA
    GATGTAGTTC TACAATGGGG AGAAATGCCT ACATCAGTTG CTTATATCCG ATCTAACCAG
    ATCATGGGAT GGGGTGAAAA AGCTATTGAG ATTCGATCCG TGGAAACTGG CCACCTTGAT
    GGAGTATTTA TGCACAAAAG GGCACAAAGG CTCAAGTTCT TGTGCGAACG AAATGACAAG
    GTTTTCTTTG CCTCGGTGCG GTCTGGCGGA AGCAGCCAAG TGTATTTCAT GACTTTGGGC
    AGAAGCTCTC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35141
    Clone ID Related Accession (Same CDS sequence) XM_012957673.2 , XM_012957673.3
    Accession Version XM_012957673.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3927bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957673.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCCAACG ATTCGCCTGC CAAAAGTCTT GTGGATATAG ACCTGTCGTC TCTGAGGGAC 
    CCAGCTGGCA TCTTTGAATT AGTGGAAGTG GTAGGAAATG GCACCTATGG ACAAGTATAT
    AAGGGCCGAC ATGTCAAAAC AGGCCAACTT GCAGCCATCA AAGTCATGGA TGTTACAGAG
    GATGAAGAGG AAGAAATCAA ACTGGAAATC AATATGTTGA AGAAATATTC CCACCACAGA
    AATATAGCTA CTTATTATGG TGCTTTTATT AAGAAAAGTC CTCCTGGGCA TGATGACCAG
    CTTTGGTTGG TGATGGAGTT TTGTGGTGCT GGCTCAATCA CTGACCTGGT GAAGAATACA
    AAGGGGAATA GTTTGAAAGA AGACTGGATT GCCTATATCT CTCGTGAGAT ACTTAGGGGA
    TTGGCCCACC TCCATGCTCA TCATGTGATT CACCGGGATA TCAAAGGACA GAATGTGCTG
    CTAACAGAGA ATGCCGAAGT GAAATTAGTG GATTTTGGTG TTAGTGCACA GCTGGATAGG
    ACAGTGGGCC GAAGGAACAC ATTTATTGGT ACACCATACT GGATGGCTCC AGAAGTTATC
    GCCTGTGATG AGAACCCAGA TGCTACCTAT GACTATAGGA GTGACCTCTG GTCATGTGGT
    ATTACAGCGA TTGAAATGGC AGAAGGTGCT CCTCCTCTTT GCGATATGCA TCCAATGCGA
    GCCCTGTTCC TTATCCCAAG AAATCCTCCA CCACGGCTAA AATCCAAGAA ATGGTCAAAG
    AAGTTCTTCA GCTTTATAGA AGGCTGCCTT GTGAAAAATT ACATGCAACG TCCCCCAACA
    GAACAGCTTT TAAAGCATCC ATTTATACGA GATCAGCCAA ATGAAAGGCA AGTCCGAATT
    CAGCTGAAGG ATCACATAGA CAGGACAAGG AAGAAGAGAG GAGAAAAAGA TGAAACAGAG
    TATGAATACA GTGGAAGTGA AGAGGAGGAA GAGGAAGTGC CTGAACAGGA AGGAGAACCA
    AGTTCCATTG TTAATGTGCC TGGGGAATCA ACACTCCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGCTC TGAGGCTTTG CGACGGCAAC AGCTGCTGCA GGAGCAACAA
    CTCAGAGAGC AGGAGGAATA TAAAAGACAG CTCCTGGCAG AGAGACAGAA GCGCATAGAA
    CAGCAGAAAG AGCAAAGGAG GAGACTTGAA GAGCAACAAA GAAGAGAGCG TGAGGCTCGT
    CGGCAGCAGG AACGAGAACA AAGAAGGCGA GAGCAAGAGG AAAAGAAGAG AATGGAGGAG
    ATGGAAAGAA GGCGCAAGGA AGAGGAAGAA AGGAGAAGAG CAGAGGAAGA GAAAAGAAGA
    GTGGAAAGAG AGCAGGAGTA TATCCGAAGA CAGCTGGAGG AGGAGCAGCG GCACTTAGAA
    ATCCTTCAGC AGCAGCTGCT TCAGGAGCAG GCCATGTTAC TGGAGTATAG ATGGCGTGAA
    ATGGAGGAGC ACCGCCAGGC TGAAAGACTT CAGCGTCAGT TGCAGCAAGA GCAGGCCTAT
    CTCATGTCAC TACAGCATGA TCATAGACAG CAGCAGCCAC CACAACAGCA GCCCCAGCCC
    CAGCAGCCTC CACTGTCCCA GCAGGAAAGG AGCAAGCAAA CATACCATAC CCAAGAACCC
    AAGCCTCATT ATGAACAAGC AGAACGAATA CGAGAGGCAG AGGAGCGCCT TAGGAAAGCA
    AACCAAGGCT CCCCTGAAGC CCAGGCTAAG CAACCAGGAA GAGTTTTAGA GCCATTAGTG
    CCTTCAAGAT CCGAATCATT TTCCAATGGA AATTCTGAGC CTGCTCAACC TGCCATGCAC
    AGACCAGTGG AACCACAGGT GCCTGTGAGG ACAACATCAA GATCTCCAGT ATTGTCACGG
    AAAGATTCAC CACTGCAGGG CAGTGGGCAG CAAAATACCC AAGCTGGTCA GAGAAATTCC
    ACAAGCAATA TTGAGCCTAG ACTTCTTTGG GAAAGAGTAG AGAAGCTTGT GCCCAGACCG
    GGTAGTGGTA GCTCGTCAGG ATCAAGTAAC TCTGGATCCC AAGCAGGATC CCACCCAGGA
    TCTCAGAGTG GATCTGGAGA GCGCTTCCGG ATGAGATCAT CTTCAAAATC TGAGGGCTCA
    CCATCTCAAC GACATGAAAA CACAGCCAAA AAGTCTGAAG AGAAGAAAGA AGCAGTTAGA
    CAGGCCAAAG CTCCTGGAGA AGTGGATCTT ACTGCACTCG CTAAGGAGCT TCGAGCAGTA
    GATGATGTTC GGCCACTTCA CAAAGTTACT GACTATTCCT CATCCAGTGA AGAGTCTGGT
    ACTACAGATG AGGAGGATGA TGACATGGAA CAGGAAGGAG CAGATGAATC GACTTCAGGC
    CCAGAGGATG CTAGAGCTAT GTCATCTTTG AATTTAAGCA ATGGAGAAAC GGAGTCTGTG
    AAAACCATGA TCGTACATGA TGATGTCGAA AGTGAACCTG CCATGACCCC ATCTAAAGAA
    GGCACTTTAA TTGTCCGACA GAGTACAGTT GACAAAAAGC GTGCCAACCA ACTTGAGAGC
    AATGGCTTTG CGAGTCGTAT TCACCTCTTG CCAGATCTCT TACAGCAAAG CCATTCCTCC
    TCCACCACTT CTTCCCCAAC CTCAAGTCAA CCGACACCTA CCATGTCCCC CCAGACACCC
    CAGGACAAGC TAACTGCTAA TGAGACTCAG TCAGCTAGTA ATACTCTCCA GAAACACAAA
    TCGTCCTCCT CTTTCACACC GTTCATAGAT CCAAGGTTAC TTCAGATCTC TCCATCTAGT
    GGAACAACTG TTACGTCAGT AGTGGGATTT TCCAGTGAAG CGCTAAGGCC GGATGCCATG
    AGGCAAGATC CTACAAGGAA AGGATCAGTT GTTAATGTTA ATCCCACAAA TACTCGGCCA
    CAAAGTGATA CACCAGAGAT TCGCAAATAC AAGAAGAGAT TTAATTCTGA GATTTTGTGT
    GCGGCATTGT GGGGTGTAAA TTTGCTGGTG GGAACTGAAA GTGGTTTAAT GCTGTTGGAT
    AGAAGTGGAC AAGGGAAAGT GTACCCCCTC ATTAACAGAA GGCGATTTCA GCAGATGGAT
    GTGCTTGAAG GCTTGAATGT CTTGGTAACT ATATCAGGGA AAAAGAACAA GCTGAGAGTA
    TATTACTTAT CCTGGCTGAG GAATAAAATT TTGCACAATG ATCCTGAAGT AGAGAAGAAA
    CAAGGATGGA CAACAGTTGG GGATTTGGAA GGCTGTGTGC ACTACAAAGT TGTAAAATAT
    GAACGTATCA AGTTTTTGGT AATCGCTTTA AAAAGTTCAG TGGAGGTGTA TGCCTGGGCA
    CCGAAACCAT ACCACAAGTT TATGGCCTTT AAGTCATTCG GAGATTTAGT ACACAGACCT
    CTCCTTGTAG ACCTGACTGT GGAAGAAGGA CAGAGGCTGA AAGTGATCTA TGGTTCCTGT
    GCTGGGTTTC ATGCAGTAGA TGTGGACTCG GGCGCAGTTT ATGATATTTA CTTGCCAACA
    CATATTCAGA GCAGCATTCA GCCACACGCA ATCATCATTC TCCCAAATAC TGATGGTATG
    GAACTGCTGG TTTGCTATGA AGATGAAGGA GTTTATGTTA ATACATACGG CAGGATCACC
    AAAGATGTAG TTCTACAATG GGGAGAAATG CCTACATCAG TTGCTTATAT CCGATCTAAC
    CAGATCATGG GATGGGGTGA AAAAGCTATT GAGATTCGAT CCGTGGAAAC TGGCCACCTT
    GATGGAGTAT TTATGCACAA AAGGGCACAA AGGCTCAAGT TCTTGTGCGA ACGAAATGAC
    AAGGTTTTCT TTGCCTCGGT GCGGTCTGGC GGAAGCAGCC AAGTGTATTT CATGACTTTG
    GGCAGAAGCT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813127.1
    CDS348..4274
    Translation

    Target ORF information:

    RefSeq Version XM_012957673.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 4 (map4k4), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957673.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCCAACG ATTCGCCTGC CAAAAGTCTT GTGGATATAG ACCTGTCGTC TCTGAGGGAC 
    CCAGCTGGCA TCTTTGAATT AGTGGAAGTG GTAGGAAATG GCACCTATGG ACAAGTATAT
    AAGGGCCGAC ATGTCAAAAC AGGCCAACTT GCAGCCATCA AAGTCATGGA TGTTACAGAG
    GATGAAGAGG AAGAAATCAA ACTGGAAATC AATATGTTGA AGAAATATTC CCACCACAGA
    AATATAGCTA CTTATTATGG TGCTTTTATT AAGAAAAGTC CTCCTGGGCA TGATGACCAG
    CTTTGGTTGG TGATGGAGTT TTGTGGTGCT GGCTCAATCA CTGACCTGGT GAAGAATACA
    AAGGGGAATA GTTTGAAAGA AGACTGGATT GCCTATATCT CTCGTGAGAT ACTTAGGGGA
    TTGGCCCACC TCCATGCTCA TCATGTGATT CACCGGGATA TCAAAGGACA GAATGTGCTG
    CTAACAGAGA ATGCCGAAGT GAAATTAGTG GATTTTGGTG TTAGTGCACA GCTGGATAGG
    ACAGTGGGCC GAAGGAACAC ATTTATTGGT ACACCATACT GGATGGCTCC AGAAGTTATC
    GCCTGTGATG AGAACCCAGA TGCTACCTAT GACTATAGGA GTGACCTCTG GTCATGTGGT
    ATTACAGCGA TTGAAATGGC AGAAGGTGCT CCTCCTCTTT GCGATATGCA TCCAATGCGA
    GCCCTGTTCC TTATCCCAAG AAATCCTCCA CCACGGCTAA AATCCAAGAA ATGGTCAAAG
    AAGTTCTTCA GCTTTATAGA AGGCTGCCTT GTGAAAAATT ACATGCAACG TCCCCCAACA
    GAACAGCTTT TAAAGCATCC ATTTATACGA GATCAGCCAA ATGAAAGGCA AGTCCGAATT
    CAGCTGAAGG ATCACATAGA CAGGACAAGG AAGAAGAGAG GAGAAAAAGA TGAAACAGAG
    TATGAATACA GTGGAAGTGA AGAGGAGGAA GAGGAAGTGC CTGAACAGGA AGGAGAACCA
    AGTTCCATTG TTAATGTGCC TGGGGAATCA ACACTCCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGCTC TGAGGCTTTG CGACGGCAAC AGCTGCTGCA GGAGCAACAA
    CTCAGAGAGC AGGAGGAATA TAAAAGACAG CTCCTGGCAG AGAGACAGAA GCGCATAGAA
    CAGCAGAAAG AGCAAAGGAG GAGACTTGAA GAGCAACAAA GAAGAGAGCG TGAGGCTCGT
    CGGCAGCAGG AACGAGAACA AAGAAGGCGA GAGCAAGAGG AAAAGAAGAG AATGGAGGAG
    ATGGAAAGAA GGCGCAAGGA AGAGGAAGAA AGGAGAAGAG CAGAGGAAGA GAAAAGAAGA
    GTGGAAAGAG AGCAGGAGTA TATCCGAAGA CAGCTGGAGG AGGAGCAGCG GCACTTAGAA
    ATCCTTCAGC AGCAGCTGCT TCAGGAGCAG GCCATGTTAC TGGAGTATAG ATGGCGTGAA
    ATGGAGGAGC ACCGCCAGGC TGAAAGACTT CAGCGTCAGT TGCAGCAAGA GCAGGCCTAT
    CTCATGTCAC TACAGCATGA TCATAGACAG CAGCAGCCAC CACAACAGCA GCCCCAGCCC
    CAGCAGCCTC CACTGTCCCA GCAGGAAAGG AGCAAGCAAA CATACCATAC CCAAGAACCC
    AAGCCTCATT ATGAACAAGC AGAACGAATA CGAGAGGCAG AGGAGCGCCT TAGGAAAGCA
    AACCAAGGCT CCCCTGAAGC CCAGGCTAAG CAACCAGGAA GAGTTTTAGA GCCATTAGTG
    CCTTCAAGAT CCGAATCATT TTCCAATGGA AATTCTGAGC CTGCTCAACC TGCCATGCAC
    AGACCAGTGG AACCACAGGT GCCTGTGAGG ACAACATCAA GATCTCCAGT ATTGTCACGG
    AAAGATTCAC CACTGCAGGG CAGTGGGCAG CAAAATACCC AAGCTGGTCA GAGAAATTCC
    ACAAGCAATA TTGAGCCTAG ACTTCTTTGG GAAAGAGTAG AGAAGCTTGT GCCCAGACCG
    GGTAGTGGTA GCTCGTCAGG ATCAAGTAAC TCTGGATCCC AAGCAGGATC CCACCCAGGA
    TCTCAGAGTG GATCTGGAGA GCGCTTCCGG ATGAGATCAT CTTCAAAATC TGAGGGCTCA
    CCATCTCAAC GACATGAAAA CACAGCCAAA AAGTCTGAAG AGAAGAAAGA AGCAGTTAGA
    CAGGCCAAAG CTCCTGGAGA AGTGGATCTT ACTGCACTCG CTAAGGAGCT TCGAGCAGTA
    GATGATGTTC GGCCACTTCA CAAAGTTACT GACTATTCCT CATCCAGTGA AGAGTCTGGT
    ACTACAGATG AGGAGGATGA TGACATGGAA CAGGAAGGAG CAGATGAATC GACTTCAGGC
    CCAGAGGATG CTAGAGCTAT GTCATCTTTG AATTTAAGCA ATGGAGAAAC GGAGTCTGTG
    AAAACCATGA TCGTACATGA TGATGTCGAA AGTGAACCTG CCATGACCCC ATCTAAAGAA
    GGCACTTTAA TTGTCCGACA GAGTACAGTT GACAAAAAGC GTGCCAACCA ACTTGAGAGC
    AATGGCTTTG CGAGTCGTAT TCACCTCTTG CCAGATCTCT TACAGCAAAG CCATTCCTCC
    TCCACCACTT CTTCCCCAAC CTCAAGTCAA CCGACACCTA CCATGTCCCC CCAGACACCC
    CAGGACAAGC TAACTGCTAA TGAGACTCAG TCAGCTAGTA ATACTCTCCA GAAACACAAA
    TCGTCCTCCT CTTTCACACC GTTCATAGAT CCAAGGTTAC TTCAGATCTC TCCATCTAGT
    GGAACAACTG TTACGTCAGT AGTGGGATTT TCCAGTGAAG CGCTAAGGCC GGATGCCATG
    AGGCAAGATC CTACAAGGAA AGGATCAGTT GTTAATGTTA ATCCCACAAA TACTCGGCCA
    CAAAGTGATA CACCAGAGAT TCGCAAATAC AAGAAGAGAT TTAATTCTGA GATTTTGTGT
    GCGGCATTGT GGGGTGTAAA TTTGCTGGTG GGAACTGAAA GTGGTTTAAT GCTGTTGGAT
    AGAAGTGGAC AAGGGAAAGT GTACCCCCTC ATTAACAGAA GGCGATTTCA GCAGATGGAT
    GTGCTTGAAG GCTTGAATGT CTTGGTAACT ATATCAGGGA AAAAGAACAA GCTGAGAGTA
    TATTACTTAT CCTGGCTGAG GAATAAAATT TTGCACAATG ATCCTGAAGT AGAGAAGAAA
    CAAGGATGGA CAACAGTTGG GGATTTGGAA GGCTGTGTGC ACTACAAAGT TGTAAAATAT
    GAACGTATCA AGTTTTTGGT AATCGCTTTA AAAAGTTCAG TGGAGGTGTA TGCCTGGGCA
    CCGAAACCAT ACCACAAGTT TATGGCCTTT AAGTCATTCG GAGATTTAGT ACACAGACCT
    CTCCTTGTAG ACCTGACTGT GGAAGAAGGA CAGAGGCTGA AAGTGATCTA TGGTTCCTGT
    GCTGGGTTTC ATGCAGTAGA TGTGGACTCG GGCGCAGTTT ATGATATTTA CTTGCCAACA
    CATATTCAGA GCAGCATTCA GCCACACGCA ATCATCATTC TCCCAAATAC TGATGGTATG
    GAACTGCTGG TTTGCTATGA AGATGAAGGA GTTTATGTTA ATACATACGG CAGGATCACC
    AAAGATGTAG TTCTACAATG GGGAGAAATG CCTACATCAG TTGCTTATAT CCGATCTAAC
    CAGATCATGG GATGGGGTGA AAAAGCTATT GAGATTCGAT CCGTGGAAAC TGGCCACCTT
    GATGGAGTAT TTATGCACAA AAGGGCACAA AGGCTCAAGT TCTTGTGCGA ACGAAATGAC
    AAGGTTTTCT TTGCCTCGGT GCGGTCTGGC GGAAGCAGCC AAGTGTATTT CATGACTTTG
    GGCAGAAGCT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa05830
    Clone ID Related Accession (Same CDS sequence) XM_002934506.4 , XM_002934506.5
    Accession Version XM_002934506.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4212bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002934506.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCCAACG ATTCGCCTGC CAAAAGTCTT GTGGATATAG ACCTGTCGTC TCTGAGGGAC 
    CCAGCTGGCA TCTTTGAATT AGTGGAAGTG GTAGGAAATG GCACCTATGG ACAAGTATAT
    AAGGGCCGAC ATGTCAAAAC AGGCCAACTT GCAGCCATCA AAGTCATGGA TGTTACAGAG
    GATGAAGAGG AAGAAATCAA ACTGGAAATC AATATGTTGA AGAAATATTC CCACCACAGA
    AATATAGCTA CTTATTATGG TGCTTTTATT AAGAAAAGTC CTCCTGGGCA TGATGACCAG
    CTTTGGTTGG TGATGGAGTT TTGTGGTGCT GGCTCAATCA CTGACCTGGT GAAGAATACA
    AAGGGGAATA GTTTGAAAGA AGACTGGATT GCCTATATCT CTCGTGAGAT ACTTAGGGGA
    TTGGCCCACC TCCATGCTCA TCATGTGATT CACCGGGATA TCAAAGGACA GAATGTGCTG
    CTAACAGAGA ATGCCGAAGT GAAATTAGTG GATTTTGGTG TTAGTGCACA GCTGGATAGG
    ACAGTGGGCC GAAGGAACAC ATTTATTGGT ACACCATACT GGATGGCTCC AGAAGTTATC
    GCCTGTGATG AGAACCCAGA TGCTACCTAT GACTATAGGA GTGACCTCTG GTCATGTGGT
    ATTACAGCGA TTGAAATGGC AGAAGGTGCT CCTCCTCTTT GCGATATGCA TCCAATGCGA
    GCCCTGTTCC TTATCCCAAG AAATCCTCCA CCACGGCTAA AATCCAAGAA ATGGTCAAAG
    AAGTTCTTCA GCTTTATAGA AGGCTGCCTT GTGAAAAATT ACATGCAACG TCCCCCAACA
    GAACAGCTTT TAAAGCATCC ATTTATACGA GATCAGCCAA ATGAAAGGCA AGTCCGAATT
    CAGCTGAAGG ATCACATAGA CAGGACAAGG AAGAAGAGAG GAGAAAAAGA TGAAACAGAG
    TATGAATACA GTGGAAGTGA AGAGGAGGAA GAGGAAGTGC CTGAACAGGA AGGAGAACCA
    AGTTCCATTG TTAATGTGCC TGGGGAATCA ACACTCCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGCTC TGAGGCTTTG CGACGGCAAC AGCTGCTGCA GGAGCAACAA
    CTCAGAGAGC AGGAGGAATA TAAAAGACAG CTCCTGGCAG AGAGACAGAA GCGCATAGAA
    CAGCAGAAAG AGCAAAGGAG GAGACTTGAA GAGCAACAAA GAAGAGAGCG TGAGGCTCGT
    CGGCAGCAGG AACGAGAACA AAGAAGGCGA GAGCAAGAGG AAAAGAAGAG AATGGAGGAG
    ATGGAAAGAA GGCGCAAGGA AGAGGAAGAA AGGAGAAGAG CAGAGGAAGA GAAAAGAAGA
    GTGGAAAGAG AGCAGGAGTA TATCCGAAGA CAGCTGGAGG AGGAGCAGCG GCACTTAGAA
    ATCCTTCAGC AGCAGCTGCT TCAGGAGCAG GCCATGTTAC TGGAGTATAG ATGGCGTGAA
    ATGGAGGAGC ACCGCCAGGC TGAAAGACTT CAGCGTCAGT TGCAGCAAGA GCAGGCCTAT
    CTCATGTCAC TACAGCATGA TCATAGACAG CAGCAGCCAC CACAACAGCA GCCCCAGCCC
    CAGCAGCCTC CACTGTCCCA GCAGGAAAGG AGCAAGCAAA CATACCATAC CCAAGAACCC
    AAGCCTCATT ATGAACAAGC AGAACGAATA CGAGAGGCAG AGGAGCGCCT TAGGAAAGCA
    AACCAAGGCT CCCCTGAAGC CCAGGCTAAG CAACCAGGAA GAGTTTTAGA GCCATTAGTG
    CCTTCAAGAT CCGAATCATT TTCCAATGGA AATTCTGAGC CTGCTCAACC TGCCATGCAC
    AGACCAGTGG AACCACAGGT ACAGTGGTCT CATCTGGCAT CCCTCAAGAA CAATGTATCC
    CCTGTCTCGC GATCCCATTC CTTCAGTGAC CCTTCTCCCA AATTTGCACC TCACCTTCTA
    CGATCTCAGG ATTCGCACCA AACTTCACGC AGTGAAGTGC AAAGTCAGGG TTCTGACCCT
    AAGTCTGAAA TCTCTGAGCC AACCCAAAGG GTTTGGGCTC GATCAGACAG TGACGAGGTG
    CCTCCAAGGG TGCCTGTGAG GACAACATCA AGATCTCCAG TATTGTCACG GAAAGATTCA
    CCACTGCAGG GCAGTGGGCA GCAAAATACC CAAGCTGGTC AGAGAAATTC CACAAGCAAT
    ATTGAGCCTA GACTTCTTTG GGAAAGAGTA GAGAAGCTTG TGCCCAGACC GGGTAGTGGT
    AGCTCGTCAG GATCAAGTAA CTCTGGATCC CAAGCAGGAT CCCACCCAGG ATCTCAGAGT
    GGATCTGGAG AGCGCTTCCG GATGAGATCA TCTTCAAAAT CTGAGGGCTC ACCATCTCAA
    CGACATGAAA ACACAGCCAA AAAGTCTGAA GAGAAGAAAG AAGCAGTTAG ACAGGCCAAA
    GCTCCTGGAG AAGTGGATCT TACTGCACTC GCTAAGGAGC TTCGAGCAGT AGATGATGTT
    CGGCCACTTC ACAAAGTTAC TGACTATTCC TCATCCAGTG AAGAGTCTGG TACTACAGAT
    GAGGAGGATG ATGACATGGA ACAGGAAGGA GCAGATGAAT CGACTTCAGG CCCAGAGGAT
    GCTAGAGCTA TGTCATCTTT GAATTTAAGC AATGGAGAAA CGGAGTCTGT GAAAACCATG
    ATCGTACATG ATGATGTCGA AAGTGAACCT GCCATGACCC CATCTAAAGA AGGCACTTTA
    ATTGTCCGAC AGAGTACAGT TGACAAAAAG CGTGCCAACC AACTTGAGAG CAATGGCTTT
    GCGAGTCGTA TTCACCTCTT GCCAGATCTC TTACAGCAAA GCCATTCCTC CTCCACCACT
    TCTTCCCCAA CCTCAAGTCA ACCGACACCT ACCATGTCCC CCCAGACACC CCAGGACAAG
    CTAACTGCTA ATGAGACTCA GTCAGCTAGT AATACTCTCC AGAAACACAA ATCGTCCTCC
    TCTTTCACAC CGTTCATAGA TCCAAGGTTA CTTCAGATCT CTCCATCTAG TGGAACAACT
    GTTACGTCAG TAGGTACATA CATGCGCACA CTGGAAGCTA ACCTTAAAAA TACTAATTTT
    ATTTTAGTGG GATTTTCCAG TGAAGCGCTA AGGCCGGATG CCATGAGGCA AGATCCTACA
    AGGAAAGGAT CAGTTGTTAA TGTTAATCCC ACAAATACTC GGCCACAAAG TGATACACCA
    GAGATTCGCA AATACAAGAA GAGATTTAAT TCTGAGATTT TGTGTGCGGC ATTGTGGGGT
    GTAAATTTGC TGGTGGGAAC TGAAAGTGGT TTAATGCTGT TGGATAGAAG TGGACAAGGG
    AAAGTGTACC CCCTCATTAA CAGAAGGCGA TTTCAGCAGA TGGATGTGCT TGAAGGCTTG
    AATGTCTTGG TAACTATATC AGGGAAAAAG AACAAGCTGA GAGTATATTA CTTATCCTGG
    CTGAGGAATA AAATTTTGCA CAATGATCCT GAAGTAGAGA AGAAACAAGG ATGGACAACA
    GTTGGGGATT TGGAAGGCTG TGTGCACTAC AAAGTTGTAA AATATGAACG TATCAAGTTT
    TTGGTAATCG CTTTAAAAAG TTCAGTGGAG GTGTATGCCT GGGCACCGAA ACCATACCAC
    AAGTTTATGG CCTTTAAGTC ATTCGGAGAT TTAGTACACA GACCTCTCCT TGTAGACCTG
    ACTGTGGAAG AAGGACAGAG GCTGAAAGTG ATCTATGGTT CCTGTGCTGG GTTTCATGCA
    GTAGATGTGG ACTCGGGCGC AGTTTATGAT ATTTACTTGC CAACACATAT TCAGAGCAGC
    ATTCAGCCAC ACGCAATCAT CATTCTCCCA AATACTGATG GTATGGAACT GCTGGTTTGC
    TATGAAGATG AAGGAGTTTA TGTTAATACA TACGGCAGGA TCACCAAAGA TGTAGTTCTA
    CAATGGGGAG AAATGCCTAC ATCAGTTGCT TATATCCGAT CTAACCAGAT CATGGGATGG
    GGTGAAAAAG CTATTGAGAT TCGATCCGTG GAAACTGGCC ACCTTGATGG AGTATTTATG
    CACAAAAGGG CACAAAGGCT CAAGTTCTTG TGCGAACGAA ATGACAAGGT TTTCTTTGCC
    TCGGTGCGGT CTGGCGGAAG CAGCCAAGTG TATTTCATGA CTTTGGGCAG AAGCTCTCTT
    CTGAGCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002934552.1
    CDS347..4558
    Translation

    Target ORF information:

    RefSeq Version XM_002934506.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 4 (map4k4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002934506.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCCAACG ATTCGCCTGC CAAAAGTCTT GTGGATATAG ACCTGTCGTC TCTGAGGGAC 
    CCAGCTGGCA TCTTTGAATT AGTGGAAGTG GTAGGAAATG GCACCTATGG ACAAGTATAT
    AAGGGCCGAC ATGTCAAAAC AGGCCAACTT GCAGCCATCA AAGTCATGGA TGTTACAGAG
    GATGAAGAGG AAGAAATCAA ACTGGAAATC AATATGTTGA AGAAATATTC CCACCACAGA
    AATATAGCTA CTTATTATGG TGCTTTTATT AAGAAAAGTC CTCCTGGGCA TGATGACCAG
    CTTTGGTTGG TGATGGAGTT TTGTGGTGCT GGCTCAATCA CTGACCTGGT GAAGAATACA
    AAGGGGAATA GTTTGAAAGA AGACTGGATT GCCTATATCT CTCGTGAGAT ACTTAGGGGA
    TTGGCCCACC TCCATGCTCA TCATGTGATT CACCGGGATA TCAAAGGACA GAATGTGCTG
    CTAACAGAGA ATGCCGAAGT GAAATTAGTG GATTTTGGTG TTAGTGCACA GCTGGATAGG
    ACAGTGGGCC GAAGGAACAC ATTTATTGGT ACACCATACT GGATGGCTCC AGAAGTTATC
    GCCTGTGATG AGAACCCAGA TGCTACCTAT GACTATAGGA GTGACCTCTG GTCATGTGGT
    ATTACAGCGA TTGAAATGGC AGAAGGTGCT CCTCCTCTTT GCGATATGCA TCCAATGCGA
    GCCCTGTTCC TTATCCCAAG AAATCCTCCA CCACGGCTAA AATCCAAGAA ATGGTCAAAG
    AAGTTCTTCA GCTTTATAGA AGGCTGCCTT GTGAAAAATT ACATGCAACG TCCCCCAACA
    GAACAGCTTT TAAAGCATCC ATTTATACGA GATCAGCCAA ATGAAAGGCA AGTCCGAATT
    CAGCTGAAGG ATCACATAGA CAGGACAAGG AAGAAGAGAG GAGAAAAAGA TGAAACAGAG
    TATGAATACA GTGGAAGTGA AGAGGAGGAA GAGGAAGTGC CTGAACAGGA AGGAGAACCA
    AGTTCCATTG TTAATGTGCC TGGGGAATCA ACACTCCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGCTC TGAGGCTTTG CGACGGCAAC AGCTGCTGCA GGAGCAACAA
    CTCAGAGAGC AGGAGGAATA TAAAAGACAG CTCCTGGCAG AGAGACAGAA GCGCATAGAA
    CAGCAGAAAG AGCAAAGGAG GAGACTTGAA GAGCAACAAA GAAGAGAGCG TGAGGCTCGT
    CGGCAGCAGG AACGAGAACA AAGAAGGCGA GAGCAAGAGG AAAAGAAGAG AATGGAGGAG
    ATGGAAAGAA GGCGCAAGGA AGAGGAAGAA AGGAGAAGAG CAGAGGAAGA GAAAAGAAGA
    GTGGAAAGAG AGCAGGAGTA TATCCGAAGA CAGCTGGAGG AGGAGCAGCG GCACTTAGAA
    ATCCTTCAGC AGCAGCTGCT TCAGGAGCAG GCCATGTTAC TGGAGTATAG ATGGCGTGAA
    ATGGAGGAGC ACCGCCAGGC TGAAAGACTT CAGCGTCAGT TGCAGCAAGA GCAGGCCTAT
    CTCATGTCAC TACAGCATGA TCATAGACAG CAGCAGCCAC CACAACAGCA GCCCCAGCCC
    CAGCAGCCTC CACTGTCCCA GCAGGAAAGG AGCAAGCAAA CATACCATAC CCAAGAACCC
    AAGCCTCATT ATGAACAAGC AGAACGAATA CGAGAGGCAG AGGAGCGCCT TAGGAAAGCA
    AACCAAGGCT CCCCTGAAGC CCAGGCTAAG CAACCAGGAA GAGTTTTAGA GCCATTAGTG
    CCTTCAAGAT CCGAATCATT TTCCAATGGA AATTCTGAGC CTGCTCAACC TGCCATGCAC
    AGACCAGTGG AACCACAGGT ACAGTGGTCT CATCTGGCAT CCCTCAAGAA CAATGTATCC
    CCTGTCTCGC GATCCCATTC CTTCAGTGAC CCTTCTCCCA AATTTGCACC TCACCTTCTA
    CGATCTCAGG ATTCGCACCA AACTTCACGC AGTGAAGTGC AAAGTCAGGG TTCTGACCCT
    AAGTCTGAAA TCTCTGAGCC AACCCAAAGG GTTTGGGCTC GATCAGACAG TGACGAGGTG
    CCTCCAAGGG TGCCTGTGAG GACAACATCA AGATCTCCAG TATTGTCACG GAAAGATTCA
    CCACTGCAGG GCAGTGGGCA GCAAAATACC CAAGCTGGTC AGAGAAATTC CACAAGCAAT
    ATTGAGCCTA GACTTCTTTG GGAAAGAGTA GAGAAGCTTG TGCCCAGACC GGGTAGTGGT
    AGCTCGTCAG GATCAAGTAA CTCTGGATCC CAAGCAGGAT CCCACCCAGG ATCTCAGAGT
    GGATCTGGAG AGCGCTTCCG GATGAGATCA TCTTCAAAAT CTGAGGGCTC ACCATCTCAA
    CGACATGAAA ACACAGCCAA AAAGTCTGAA GAGAAGAAAG AAGCAGTTAG ACAGGCCAAA
    GCTCCTGGAG AAGTGGATCT TACTGCACTC GCTAAGGAGC TTCGAGCAGT AGATGATGTT
    CGGCCACTTC ACAAAGTTAC TGACTATTCC TCATCCAGTG AAGAGTCTGG TACTACAGAT
    GAGGAGGATG ATGACATGGA ACAGGAAGGA GCAGATGAAT CGACTTCAGG CCCAGAGGAT
    GCTAGAGCTA TGTCATCTTT GAATTTAAGC AATGGAGAAA CGGAGTCTGT GAAAACCATG
    ATCGTACATG ATGATGTCGA AAGTGAACCT GCCATGACCC CATCTAAAGA AGGCACTTTA
    ATTGTCCGAC AGAGTACAGT TGACAAAAAG CGTGCCAACC AACTTGAGAG CAATGGCTTT
    GCGAGTCGTA TTCACCTCTT GCCAGATCTC TTACAGCAAA GCCATTCCTC CTCCACCACT
    TCTTCCCCAA CCTCAAGTCA ACCGACACCT ACCATGTCCC CCCAGACACC CCAGGACAAG
    CTAACTGCTA ATGAGACTCA GTCAGCTAGT AATACTCTCC AGAAACACAA ATCGTCCTCC
    TCTTTCACAC CGTTCATAGA TCCAAGGTTA CTTCAGATCT CTCCATCTAG TGGAACAACT
    GTTACGTCAG TAGGTACATA CATGCGCACA CTGGAAGCTA ACCTTAAAAA TACTAATTTT
    ATTTTAGTGG GATTTTCCAG TGAAGCGCTA AGGCCGGATG CCATGAGGCA AGATCCTACA
    AGGAAAGGAT CAGTTGTTAA TGTTAATCCC ACAAATACTC GGCCACAAAG TGATACACCA
    GAGATTCGCA AATACAAGAA GAGATTTAAT TCTGAGATTT TGTGTGCGGC ATTGTGGGGT
    GTAAATTTGC TGGTGGGAAC TGAAAGTGGT TTAATGCTGT TGGATAGAAG TGGACAAGGG
    AAAGTGTACC CCCTCATTAA CAGAAGGCGA TTTCAGCAGA TGGATGTGCT TGAAGGCTTG
    AATGTCTTGG TAACTATATC AGGGAAAAAG AACAAGCTGA GAGTATATTA CTTATCCTGG
    CTGAGGAATA AAATTTTGCA CAATGATCCT GAAGTAGAGA AGAAACAAGG ATGGACAACA
    GTTGGGGATT TGGAAGGCTG TGTGCACTAC AAAGTTGTAA AATATGAACG TATCAAGTTT
    TTGGTAATCG CTTTAAAAAG TTCAGTGGAG GTGTATGCCT GGGCACCGAA ACCATACCAC
    AAGTTTATGG CCTTTAAGTC ATTCGGAGAT TTAGTACACA GACCTCTCCT TGTAGACCTG
    ACTGTGGAAG AAGGACAGAG GCTGAAAGTG ATCTATGGTT CCTGTGCTGG GTTTCATGCA
    GTAGATGTGG ACTCGGGCGC AGTTTATGAT ATTTACTTGC CAACACATAT TCAGAGCAGC
    ATTCAGCCAC ACGCAATCAT CATTCTCCCA AATACTGATG GTATGGAACT GCTGGTTTGC
    TATGAAGATG AAGGAGTTTA TGTTAATACA TACGGCAGGA TCACCAAAGA TGTAGTTCTA
    CAATGGGGAG AAATGCCTAC ATCAGTTGCT TATATCCGAT CTAACCAGAT CATGGGATGG
    GGTGAAAAAG CTATTGAGAT TCGATCCGTG GAAACTGGCC ACCTTGATGG AGTATTTATG
    CACAAAAGGG CACAAAGGCT CAAGTTCTTG TGCGAACGAA ATGACAAGGT TTTCTTTGCC
    TCGGTGCGGT CTGGCGGAAG CAGCCAAGTG TATTTCATGA CTTTGGGCAG AAGCTCTCTT
    CTGAGCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa05831
    Clone ID Related Accession (Same CDS sequence) XM_004911783.3 , XM_004911783.4
    Accession Version XM_004911783.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4158bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004911783.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCCAACG ATTCGCCTGC CAAAAGTCTT GTGGATATAG ACCTGTCGTC TCTGAGGGAC 
    CCAGCTGGCA TCTTTGAATT AGTGGAAGTG GTAGGAAATG GCACCTATGG ACAAGTATAT
    AAGGGCCGAC ATGTCAAAAC AGGCCAACTT GCAGCCATCA AAGTCATGGA TGTTACAGAG
    GATGAAGAGG AAGAAATCAA ACTGGAAATC AATATGTTGA AGAAATATTC CCACCACAGA
    AATATAGCTA CTTATTATGG TGCTTTTATT AAGAAAAGTC CTCCTGGGCA TGATGACCAG
    CTTTGGTTGG TGATGGAGTT TTGTGGTGCT GGCTCAATCA CTGACCTGGT GAAGAATACA
    AAGGGGAATA GTTTGAAAGA AGACTGGATT GCCTATATCT CTCGTGAGAT ACTTAGGGGA
    TTGGCCCACC TCCATGCTCA TCATGTGATT CACCGGGATA TCAAAGGACA GAATGTGCTG
    CTAACAGAGA ATGCCGAAGT GAAATTAGTG GATTTTGGTG TTAGTGCACA GCTGGATAGG
    ACAGTGGGCC GAAGGAACAC ATTTATTGGT ACACCATACT GGATGGCTCC AGAAGTTATC
    GCCTGTGATG AGAACCCAGA TGCTACCTAT GACTATAGGA GTGACCTCTG GTCATGTGGT
    ATTACAGCGA TTGAAATGGC AGAAGGTGCT CCTCCTCTTT GCGATATGCA TCCAATGCGA
    GCCCTGTTCC TTATCCCAAG AAATCCTCCA CCACGGCTAA AATCCAAGAA ATGGTCAAAG
    AAGTTCTTCA GCTTTATAGA AGGCTGCCTT GTGAAAAATT ACATGCAACG TCCCCCAACA
    GAACAGCTTT TAAAGCATCC ATTTATACGA GATCAGCCAA ATGAAAGGCA AGTCCGAATT
    CAGCTGAAGG ATCACATAGA CAGGACAAGG AAGAAGAGAG GAGAAAAAGA TGAAACAGAG
    TATGAATACA GTGGAAGTGA AGAGGAGGAA GAGGAAGTGC CTGAACAGGA AGGAGAACCA
    AGTTCCATTG TTAATGTGCC TGGGGAATCA ACACTCCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGCTC TGAGGCTTTG CGACGGCAAC AGCTGCTGCA GGAGCAACAA
    CTCAGAGAGC AGGAGGAATA TAAAAGACAG CTCCTGGCAG AGAGACAGAA GCGCATAGAA
    CAGCAGAAAG AGCAAAGGAG GAGACTTGAA GAGCAACAAA GAAGAGAGCG TGAGGCTCGT
    CGGCAGCAGG AACGAGAACA AAGAAGGCGA GAGCAAGAGG AAAAGAAGAG AATGGAGGAG
    ATGGAAAGAA GGCGCAAGGA AGAGGAAGAA AGGAGAAGAG CAGAGGAAGA GAAAAGAAGA
    GTGGAAAGAG AGCAGGAGTA TATCCGAAGA CAGCTGGAGG AGGAGCAGCG GCACTTAGAA
    ATCCTTCAGC AGCAGCTGCT TCAGGAGCAG GCCATGTTAC TGGAGTATAG ATGGCGTGAA
    ATGGAGGAGC ACCGCCAGGC TGAAAGACTT CAGCGTCAGT TGCAGCAAGA GCAGGCCTAT
    CTCATGTCAC TACAGCATGA TCATAGACAG CAGCAGCCAC CACAACAGCA GCCCCAGCCC
    CAGCAGCCTC CACTGTCCCA GCAGGAAAGG AGCAAGCAAA CATACCATAC CCAAGAACCC
    AAGCCTCATT ATGAACAAGC AGAACGAATA CGAGAGGCAG AGGAGCGCCT TAGGAAAGCA
    AACCAAGGCT CCCCTGAAGC CCAGGCTAAG CAACCAGGAA GAGTTTTAGA GCCATTAGTG
    CCTTCAAGAT CCGAATCATT TTCCAATGGA AATTCTGAGC CTGCTCAACC TGCCATGCAC
    AGACCAGTGG AACCACAGGT ACAGTGGTCT CATCTGGCAT CCCTCAAGAA CAATGTATCC
    CCTGTCTCGC GATCCCATTC CTTCAGTGAC CCTTCTCCCA AATTTGCACC TCACCTTCTA
    CGATCTCAGG ATTCGCACCA AACTTCACGC AGTGAAGTGC AAAGTCAGGG TTCTGACCCT
    AAGTCTGAAA TCTCTGAGCC AACCCAAAGG GTTTGGGCTC GATCAGACAG TGACGAGGTG
    CCTCCAAGGG TGCCTGTGAG GACAACATCA AGATCTCCAG TATTGTCACG GAAAGATTCA
    CCACTGCAGG GCAGTGGGCA GCAAAATACC CAAGCTGGTC AGAGAAATTC CACAAGCAAT
    ATTGAGCCTA GACTTCTTTG GGAAAGAGTA GAGAAGCTTG TGCCCAGACC GGGTAGTGGT
    AGCTCGTCAG GATCAAGTAA CTCTGGATCC CAAGCAGGAT CCCACCCAGG ATCTCAGAGT
    GGATCTGGAG AGCGCTTCCG GATGAGATCA TCTTCAAAAT CTGAGGGCTC ACCATCTCAA
    CGACATGAAA ACACAGCCAA AAAGTCTGAA GAGAAGAAAG AAGCAGTTAG ACAGGCCAAA
    GCTCCTGGAG AAGTGGATCT TACTGCACTC GCTAAGGAGC TTCGAGCAGT AGATGATGTT
    CGGCCACTTC ACAAAGTTAC TGACTATTCC TCATCCAGTG AAGAGTCTGG TACTACAGAT
    GAGGAGGATG ATGACATGGA ACAGGAAGGA GCAGATGAAT CGACTTCAGG CCCAGAGGAT
    GCTAGAGCTA TGTCATCTTT GAATTTAAGC AATGGAGAAA CGGAGTCTGT GAAAACCATG
    ATCGTACATG ATGATGTCGA AAGTGAACCT GCCATGACCC CATCTAAAGA AGGCACTTTA
    ATTGTCCGAC AGAGTACAGT TGACAAAAAG CGTGCCAACC AACTTGAGAG CAATGGCTTT
    GCGAGTCGTA TTCACCTCTT GCCAGATCTC TTACAGCAAA GCCATTCCTC CTCCACCACT
    TCTTCCCCAA CCTCAAGTCA ACCGACACCT ACCATGTCCC CCCAGACACC CCAGGACAAG
    CTAACTGCTA ATGAGACTCA GTCAGCTAGT AATACTCTCC AGAAACACAA ATCGTCCTCC
    TCTTTCACAC CGTTCATAGA TCCAAGGTTA CTTCAGATCT CTCCATCTAG TGGAACAACT
    GTTACGTCAG TAGTGGGATT TTCCAGTGAA GCGCTAAGGC CGGATGCCAT GAGGCAAGAT
    CCTACAAGGA AAGGATCAGT TGTTAATGTT AATCCCACAA ATACTCGGCC ACAAAGTGAT
    ACACCAGAGA TTCGCAAATA CAAGAAGAGA TTTAATTCTG AGATTTTGTG TGCGGCATTG
    TGGGGTGTAA ATTTGCTGGT GGGAACTGAA AGTGGTTTAA TGCTGTTGGA TAGAAGTGGA
    CAAGGGAAAG TGTACCCCCT CATTAACAGA AGGCGATTTC AGCAGATGGA TGTGCTTGAA
    GGCTTGAATG TCTTGGTAAC TATATCAGGG AAAAAGAACA AGCTGAGAGT ATATTACTTA
    TCCTGGCTGA GGAATAAAAT TTTGCACAAT GATCCTGAAG TAGAGAAGAA ACAAGGATGG
    ACAACAGTTG GGGATTTGGA AGGCTGTGTG CACTACAAAG TTGTAAAATA TGAACGTATC
    AAGTTTTTGG TAATCGCTTT AAAAAGTTCA GTGGAGGTGT ATGCCTGGGC ACCGAAACCA
    TACCACAAGT TTATGGCCTT TAAGTCATTC GGAGATTTAG TACACAGACC TCTCCTTGTA
    GACCTGACTG TGGAAGAAGG ACAGAGGCTG AAAGTGATCT ATGGTTCCTG TGCTGGGTTT
    CATGCAGTAG ATGTGGACTC GGGCGCAGTT TATGATATTT ACTTGCCAAC ACATATTCAG
    AGCAGCATTC AGCCACACGC AATCATCATT CTCCCAAATA CTGATGGTAT GGAACTGCTG
    GTTTGCTATG AAGATGAAGG AGTTTATGTT AATACATACG GCAGGATCAC CAAAGATGTA
    GTTCTACAAT GGGGAGAAAT GCCTACATCA GTTGCTTATA TCCGATCTAA CCAGATCATG
    GGATGGGGTG AAAAAGCTAT TGAGATTCGA TCCGTGGAAA CTGGCCACCT TGATGGAGTA
    TTTATGCACA AAAGGGCACA AAGGCTCAAG TTCTTGTGCG AACGAAATGA CAAGGTTTTC
    TTTGCCTCGG TGCGGTCTGG CGGAAGCAGC CAAGTGTATT TCATGACTTT GGGCAGAAGC
    TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004911840.1
    CDS347..4504
    Translation

    Target ORF information:

    RefSeq Version XM_004911783.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 4 (map4k4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004911783.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCCAACG ATTCGCCTGC CAAAAGTCTT GTGGATATAG ACCTGTCGTC TCTGAGGGAC 
    CCAGCTGGCA TCTTTGAATT AGTGGAAGTG GTAGGAAATG GCACCTATGG ACAAGTATAT
    AAGGGCCGAC ATGTCAAAAC AGGCCAACTT GCAGCCATCA AAGTCATGGA TGTTACAGAG
    GATGAAGAGG AAGAAATCAA ACTGGAAATC AATATGTTGA AGAAATATTC CCACCACAGA
    AATATAGCTA CTTATTATGG TGCTTTTATT AAGAAAAGTC CTCCTGGGCA TGATGACCAG
    CTTTGGTTGG TGATGGAGTT TTGTGGTGCT GGCTCAATCA CTGACCTGGT GAAGAATACA
    AAGGGGAATA GTTTGAAAGA AGACTGGATT GCCTATATCT CTCGTGAGAT ACTTAGGGGA
    TTGGCCCACC TCCATGCTCA TCATGTGATT CACCGGGATA TCAAAGGACA GAATGTGCTG
    CTAACAGAGA ATGCCGAAGT GAAATTAGTG GATTTTGGTG TTAGTGCACA GCTGGATAGG
    ACAGTGGGCC GAAGGAACAC ATTTATTGGT ACACCATACT GGATGGCTCC AGAAGTTATC
    GCCTGTGATG AGAACCCAGA TGCTACCTAT GACTATAGGA GTGACCTCTG GTCATGTGGT
    ATTACAGCGA TTGAAATGGC AGAAGGTGCT CCTCCTCTTT GCGATATGCA TCCAATGCGA
    GCCCTGTTCC TTATCCCAAG AAATCCTCCA CCACGGCTAA AATCCAAGAA ATGGTCAAAG
    AAGTTCTTCA GCTTTATAGA AGGCTGCCTT GTGAAAAATT ACATGCAACG TCCCCCAACA
    GAACAGCTTT TAAAGCATCC ATTTATACGA GATCAGCCAA ATGAAAGGCA AGTCCGAATT
    CAGCTGAAGG ATCACATAGA CAGGACAAGG AAGAAGAGAG GAGAAAAAGA TGAAACAGAG
    TATGAATACA GTGGAAGTGA AGAGGAGGAA GAGGAAGTGC CTGAACAGGA AGGAGAACCA
    AGTTCCATTG TTAATGTGCC TGGGGAATCA ACACTCCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGCTC TGAGGCTTTG CGACGGCAAC AGCTGCTGCA GGAGCAACAA
    CTCAGAGAGC AGGAGGAATA TAAAAGACAG CTCCTGGCAG AGAGACAGAA GCGCATAGAA
    CAGCAGAAAG AGCAAAGGAG GAGACTTGAA GAGCAACAAA GAAGAGAGCG TGAGGCTCGT
    CGGCAGCAGG AACGAGAACA AAGAAGGCGA GAGCAAGAGG AAAAGAAGAG AATGGAGGAG
    ATGGAAAGAA GGCGCAAGGA AGAGGAAGAA AGGAGAAGAG CAGAGGAAGA GAAAAGAAGA
    GTGGAAAGAG AGCAGGAGTA TATCCGAAGA CAGCTGGAGG AGGAGCAGCG GCACTTAGAA
    ATCCTTCAGC AGCAGCTGCT TCAGGAGCAG GCCATGTTAC TGGAGTATAG ATGGCGTGAA
    ATGGAGGAGC ACCGCCAGGC TGAAAGACTT CAGCGTCAGT TGCAGCAAGA GCAGGCCTAT
    CTCATGTCAC TACAGCATGA TCATAGACAG CAGCAGCCAC CACAACAGCA GCCCCAGCCC
    CAGCAGCCTC CACTGTCCCA GCAGGAAAGG AGCAAGCAAA CATACCATAC CCAAGAACCC
    AAGCCTCATT ATGAACAAGC AGAACGAATA CGAGAGGCAG AGGAGCGCCT TAGGAAAGCA
    AACCAAGGCT CCCCTGAAGC CCAGGCTAAG CAACCAGGAA GAGTTTTAGA GCCATTAGTG
    CCTTCAAGAT CCGAATCATT TTCCAATGGA AATTCTGAGC CTGCTCAACC TGCCATGCAC
    AGACCAGTGG AACCACAGGT ACAGTGGTCT CATCTGGCAT CCCTCAAGAA CAATGTATCC
    CCTGTCTCGC GATCCCATTC CTTCAGTGAC CCTTCTCCCA AATTTGCACC TCACCTTCTA
    CGATCTCAGG ATTCGCACCA AACTTCACGC AGTGAAGTGC AAAGTCAGGG TTCTGACCCT
    AAGTCTGAAA TCTCTGAGCC AACCCAAAGG GTTTGGGCTC GATCAGACAG TGACGAGGTG
    CCTCCAAGGG TGCCTGTGAG GACAACATCA AGATCTCCAG TATTGTCACG GAAAGATTCA
    CCACTGCAGG GCAGTGGGCA GCAAAATACC CAAGCTGGTC AGAGAAATTC CACAAGCAAT
    ATTGAGCCTA GACTTCTTTG GGAAAGAGTA GAGAAGCTTG TGCCCAGACC GGGTAGTGGT
    AGCTCGTCAG GATCAAGTAA CTCTGGATCC CAAGCAGGAT CCCACCCAGG ATCTCAGAGT
    GGATCTGGAG AGCGCTTCCG GATGAGATCA TCTTCAAAAT CTGAGGGCTC ACCATCTCAA
    CGACATGAAA ACACAGCCAA AAAGTCTGAA GAGAAGAAAG AAGCAGTTAG ACAGGCCAAA
    GCTCCTGGAG AAGTGGATCT TACTGCACTC GCTAAGGAGC TTCGAGCAGT AGATGATGTT
    CGGCCACTTC ACAAAGTTAC TGACTATTCC TCATCCAGTG AAGAGTCTGG TACTACAGAT
    GAGGAGGATG ATGACATGGA ACAGGAAGGA GCAGATGAAT CGACTTCAGG CCCAGAGGAT
    GCTAGAGCTA TGTCATCTTT GAATTTAAGC AATGGAGAAA CGGAGTCTGT GAAAACCATG
    ATCGTACATG ATGATGTCGA AAGTGAACCT GCCATGACCC CATCTAAAGA AGGCACTTTA
    ATTGTCCGAC AGAGTACAGT TGACAAAAAG CGTGCCAACC AACTTGAGAG CAATGGCTTT
    GCGAGTCGTA TTCACCTCTT GCCAGATCTC TTACAGCAAA GCCATTCCTC CTCCACCACT
    TCTTCCCCAA CCTCAAGTCA ACCGACACCT ACCATGTCCC CCCAGACACC CCAGGACAAG
    CTAACTGCTA ATGAGACTCA GTCAGCTAGT AATACTCTCC AGAAACACAA ATCGTCCTCC
    TCTTTCACAC CGTTCATAGA TCCAAGGTTA CTTCAGATCT CTCCATCTAG TGGAACAACT
    GTTACGTCAG TAGTGGGATT TTCCAGTGAA GCGCTAAGGC CGGATGCCAT GAGGCAAGAT
    CCTACAAGGA AAGGATCAGT TGTTAATGTT AATCCCACAA ATACTCGGCC ACAAAGTGAT
    ACACCAGAGA TTCGCAAATA CAAGAAGAGA TTTAATTCTG AGATTTTGTG TGCGGCATTG
    TGGGGTGTAA ATTTGCTGGT GGGAACTGAA AGTGGTTTAA TGCTGTTGGA TAGAAGTGGA
    CAAGGGAAAG TGTACCCCCT CATTAACAGA AGGCGATTTC AGCAGATGGA TGTGCTTGAA
    GGCTTGAATG TCTTGGTAAC TATATCAGGG AAAAAGAACA AGCTGAGAGT ATATTACTTA
    TCCTGGCTGA GGAATAAAAT TTTGCACAAT GATCCTGAAG TAGAGAAGAA ACAAGGATGG
    ACAACAGTTG GGGATTTGGA AGGCTGTGTG CACTACAAAG TTGTAAAATA TGAACGTATC
    AAGTTTTTGG TAATCGCTTT AAAAAGTTCA GTGGAGGTGT ATGCCTGGGC ACCGAAACCA
    TACCACAAGT TTATGGCCTT TAAGTCATTC GGAGATTTAG TACACAGACC TCTCCTTGTA
    GACCTGACTG TGGAAGAAGG ACAGAGGCTG AAAGTGATCT ATGGTTCCTG TGCTGGGTTT
    CATGCAGTAG ATGTGGACTC GGGCGCAGTT TATGATATTT ACTTGCCAAC ACATATTCAG
    AGCAGCATTC AGCCACACGC AATCATCATT CTCCCAAATA CTGATGGTAT GGAACTGCTG
    GTTTGCTATG AAGATGAAGG AGTTTATGTT AATACATACG GCAGGATCAC CAAAGATGTA
    GTTCTACAAT GGGGAGAAAT GCCTACATCA GTTGCTTATA TCCGATCTAA CCAGATCATG
    GGATGGGGTG AAAAAGCTAT TGAGATTCGA TCCGTGGAAA CTGGCCACCT TGATGGAGTA
    TTTATGCACA AAAGGGCACA AAGGCTCAAG TTCTTGTGCG AACGAAATGA CAAGGTTTTC
    TTTGCCTCGG TGCGGTCTGG CGGAAGCAGC CAAGTGTATT TCATGACTTT GGGCAGAAGC
    TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa05832
    Clone ID Related Accession (Same CDS sequence) XM_004911784.3 , XM_004911784.4
    Accession Version XM_004911784.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4125bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004911784.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCCAACG ATTCGCCTGC CAAAAGTCTT GTGGATATAG ACCTGTCGTC TCTGAGGGAC 
    CCAGCTGGCA TCTTTGAATT AGTGGAAGTG GTAGGAAATG GCACCTATGG ACAAGTATAT
    AAGGGCCGAC ATGTCAAAAC AGGCCAACTT GCAGCCATCA AAGTCATGGA TGTTACAGAG
    GATGAAGAGG AAGAAATCAA ACTGGAAATC AATATGTTGA AGAAATATTC CCACCACAGA
    AATATAGCTA CTTATTATGG TGCTTTTATT AAGAAAAGTC CTCCTGGGCA TGATGACCAG
    CTTTGGTTGG TGATGGAGTT TTGTGGTGCT GGCTCAATCA CTGACCTGGT GAAGAATACA
    AAGGGGAATA GTTTGAAAGA AGACTGGATT GCCTATATCT CTCGTGAGAT ACTTAGGGGA
    TTGGCCCACC TCCATGCTCA TCATGTGATT CACCGGGATA TCAAAGGACA GAATGTGCTG
    CTAACAGAGA ATGCCGAAGT GAAATTAGTG GATTTTGGTG TTAGTGCACA GCTGGATAGG
    ACAGTGGGCC GAAGGAACAC ATTTATTGGT ACACCATACT GGATGGCTCC AGAAGTTATC
    GCCTGTGATG AGAACCCAGA TGCTACCTAT GACTATAGGA GTGACCTCTG GTCATGTGGT
    ATTACAGCGA TTGAAATGGC AGAAGGTGCT CCTCCTCTTT GCGATATGCA TCCAATGCGA
    GCCCTGTTCC TTATCCCAAG AAATCCTCCA CCACGGCTAA AATCCAAGAA ATGGTCAAAG
    AAGTTCTTCA GCTTTATAGA AGGCTGCCTT GTGAAAAATT ACATGCAACG TCCCCCAACA
    GAACAGCTTT TAAAGCATCC ATTTATACGA GATCAGCCAA ATGAAAGGCA AGTCCGAATT
    CAGCTGAAGG ATCACATAGA CAGGACAAGG AAGAAGAGAG GAGAAAAAGA TGAAACAGAG
    TATGAATACA GTGGAAGTGA AGAGGAGGAA GAGGAAGTGC CTGAACAGGA AGGAGAACCA
    AGTTCCATTG TTAATGTGCC TGGGGAATCA ACACTCCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGCTC TGAGGCTTTG CGACGGCAAC AGCTGCTGCA GGAGCAACAA
    CTCAGAGAGC AGGAGGAATA TAAAAGACAG CTCCTGGCAG AGAGACAGAA GCGCATAGAA
    CAGCAGAAAG AGCAAAGGAG GAGACTTGAA GAGCAACAAA GAAGAGAGCG TGAGGCTCGT
    CGGCAGCAGG AACGAGAACA AAGAAGGCGA GAGCAAGAGG AAAAGAAGAG AATGGAGGAG
    ATGGAAAGAA GGCGCAAGGA AGAGGAAGAA AGGAGAAGAG CAGAGGAAGA GAAAAGAAGA
    GTGGAAAGAG AGCAGGAGTA TAGATGGCGT GAAATGGAGG AGCACCGCCA GGCTGAAAGA
    CTTCAGCGTC AGTTGCAGCA AGAGCAGGCC TATCTCATGT CACTACAGCA TGATCATAGA
    CAGCAGCAGC CACCACAACA GCAGCCCCAG CCCCAGCAGC CTCCACTGTC CCAGCAGGAA
    AGGAGCAAGC AAACATACCA TACCCAAGAA CCCAAGCCTC ATTATGAACA AGCAGAACGA
    ATACGAGAGG CAGAGGAGCG CCTTAGGAAA GCAAACCAAG GCTCCCCTGA AGCCCAGGCT
    AAGCAACCAG GAAGAGTTTT AGAGCCATTA GTGCCTTCAA GATCCGAATC ATTTTCCAAT
    GGAAATTCTG AGCCTGCTCA ACCTGCCATG CACAGACCAG TGGAACCACA GGTACAGTGG
    TCTCATCTGG CATCCCTCAA GAACAATGTA TCCCCTGTCT CGCGATCCCA TTCCTTCAGT
    GACCCTTCTC CCAAATTTGC ACCTCACCTT CTACGATCTC AGGATTCGCA CCAAACTTCA
    CGCAGTGAAG TGCAAAGTCA GGGTTCTGAC CCTAAGTCTG AAATCTCTGA GCCAACCCAA
    AGGGTTTGGG CTCGATCAGA CAGTGACGAG GTGCCTCCAA GGGTGCCTGT GAGGACAACA
    TCAAGATCTC CAGTATTGTC ACGGAAAGAT TCACCACTGC AGGGCAGTGG GCAGCAAAAT
    ACCCAAGCTG GTCAGAGAAA TTCCACAAGC AATATTGAGC CTAGACTTCT TTGGGAAAGA
    GTAGAGAAGC TTGTGCCCAG ACCGGGTAGT GGTAGCTCGT CAGGATCAAG TAACTCTGGA
    TCCCAAGCAG GATCCCACCC AGGATCTCAG AGTGGATCTG GAGAGCGCTT CCGGATGAGA
    TCATCTTCAA AATCTGAGGG CTCACCATCT CAACGACATG AAAACACAGC CAAAAAGTCT
    GAAGAGAAGA AAGAAGCAGT TAGACAGGCC AAAGCTCCTG GAGAAGTGGA TCTTACTGCA
    CTCGCTAAGG AGCTTCGAGC AGTAGATGAT GTTCGGCCAC TTCACAAAGT TACTGACTAT
    TCCTCATCCA GTGAAGAGTC TGGTACTACA GATGAGGAGG ATGATGACAT GGAACAGGAA
    GGAGCAGATG AATCGACTTC AGGCCCAGAG GATGCTAGAG CTATGTCATC TTTGAATTTA
    AGCAATGGAG AAACGGAGTC TGTGAAAACC ATGATCGTAC ATGATGATGT CGAAAGTGAA
    CCTGCCATGA CCCCATCTAA AGAAGGCACT TTAATTGTCC GACAGAGTAC AGTTGACAAA
    AAGCGTGCCA ACCAACTTGA GAGCAATGGC TTTGCGAGTC GTATTCACCT CTTGCCAGAT
    CTCTTACAGC AAAGCCATTC CTCCTCCACC ACTTCTTCCC CAACCTCAAG TCAACCGACA
    CCTACCATGT CCCCCCAGAC ACCCCAGGAC AAGCTAACTG CTAATGAGAC TCAGTCAGCT
    AGTAATACTC TCCAGAAACA CAAATCGTCC TCCTCTTTCA CACCGTTCAT AGATCCAAGG
    TTACTTCAGA TCTCTCCATC TAGTGGAACA ACTGTTACGT CAGTAGGTAC ATACATGCGC
    ACACTGGAAG CTAACCTTAA AAATACTAAT TTTATTTTAG TGGGATTTTC CAGTGAAGCG
    CTAAGGCCGG ATGCCATGAG GCAAGATCCT ACAAGGAAAG GATCAGTTGT TAATGTTAAT
    CCCACAAATA CTCGGCCACA AAGTGATACA CCAGAGATTC GCAAATACAA GAAGAGATTT
    AATTCTGAGA TTTTGTGTGC GGCATTGTGG GGTGTAAATT TGCTGGTGGG AACTGAAAGT
    GGTTTAATGC TGTTGGATAG AAGTGGACAA GGGAAAGTGT ACCCCCTCAT TAACAGAAGG
    CGATTTCAGC AGATGGATGT GCTTGAAGGC TTGAATGTCT TGGTAACTAT ATCAGGGAAA
    AAGAACAAGC TGAGAGTATA TTACTTATCC TGGCTGAGGA ATAAAATTTT GCACAATGAT
    CCTGAAGTAG AGAAGAAACA AGGATGGACA ACAGTTGGGG ATTTGGAAGG CTGTGTGCAC
    TACAAAGTTG TAAAATATGA ACGTATCAAG TTTTTGGTAA TCGCTTTAAA AAGTTCAGTG
    GAGGTGTATG CCTGGGCACC GAAACCATAC CACAAGTTTA TGGCCTTTAA GTCATTCGGA
    GATTTAGTAC ACAGACCTCT CCTTGTAGAC CTGACTGTGG AAGAAGGACA GAGGCTGAAA
    GTGATCTATG GTTCCTGTGC TGGGTTTCAT GCAGTAGATG TGGACTCGGG CGCAGTTTAT
    GATATTTACT TGCCAACACA TATTCAGAGC AGCATTCAGC CACACGCAAT CATCATTCTC
    CCAAATACTG ATGGTATGGA ACTGCTGGTT TGCTATGAAG ATGAAGGAGT TTATGTTAAT
    ACATACGGCA GGATCACCAA AGATGTAGTT CTACAATGGG GAGAAATGCC TACATCAGTT
    GCTTATATCC GATCTAACCA GATCATGGGA TGGGGTGAAA AAGCTATTGA GATTCGATCC
    GTGGAAACTG GCCACCTTGA TGGAGTATTT ATGCACAAAA GGGCACAAAG GCTCAAGTTC
    TTGTGCGAAC GAAATGACAA GGTTTTCTTT GCCTCGGTGC GGTCTGGCGG AAGCAGCCAA
    GTGTATTTCA TGACTTTGGG CAGAAGCTCT CTTCTGAGCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004911841.1
    CDS348..4472
    Translation

    Target ORF information:

    RefSeq Version XM_004911784.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 4 (map4k4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004911784.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCCAACG ATTCGCCTGC CAAAAGTCTT GTGGATATAG ACCTGTCGTC TCTGAGGGAC 
    CCAGCTGGCA TCTTTGAATT AGTGGAAGTG GTAGGAAATG GCACCTATGG ACAAGTATAT
    AAGGGCCGAC ATGTCAAAAC AGGCCAACTT GCAGCCATCA AAGTCATGGA TGTTACAGAG
    GATGAAGAGG AAGAAATCAA ACTGGAAATC AATATGTTGA AGAAATATTC CCACCACAGA
    AATATAGCTA CTTATTATGG TGCTTTTATT AAGAAAAGTC CTCCTGGGCA TGATGACCAG
    CTTTGGTTGG TGATGGAGTT TTGTGGTGCT GGCTCAATCA CTGACCTGGT GAAGAATACA
    AAGGGGAATA GTTTGAAAGA AGACTGGATT GCCTATATCT CTCGTGAGAT ACTTAGGGGA
    TTGGCCCACC TCCATGCTCA TCATGTGATT CACCGGGATA TCAAAGGACA GAATGTGCTG
    CTAACAGAGA ATGCCGAAGT GAAATTAGTG GATTTTGGTG TTAGTGCACA GCTGGATAGG
    ACAGTGGGCC GAAGGAACAC ATTTATTGGT ACACCATACT GGATGGCTCC AGAAGTTATC
    GCCTGTGATG AGAACCCAGA TGCTACCTAT GACTATAGGA GTGACCTCTG GTCATGTGGT
    ATTACAGCGA TTGAAATGGC AGAAGGTGCT CCTCCTCTTT GCGATATGCA TCCAATGCGA
    GCCCTGTTCC TTATCCCAAG AAATCCTCCA CCACGGCTAA AATCCAAGAA ATGGTCAAAG
    AAGTTCTTCA GCTTTATAGA AGGCTGCCTT GTGAAAAATT ACATGCAACG TCCCCCAACA
    GAACAGCTTT TAAAGCATCC ATTTATACGA GATCAGCCAA ATGAAAGGCA AGTCCGAATT
    CAGCTGAAGG ATCACATAGA CAGGACAAGG AAGAAGAGAG GAGAAAAAGA TGAAACAGAG
    TATGAATACA GTGGAAGTGA AGAGGAGGAA GAGGAAGTGC CTGAACAGGA AGGAGAACCA
    AGTTCCATTG TTAATGTGCC TGGGGAATCA ACACTCCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGCTC TGAGGCTTTG CGACGGCAAC AGCTGCTGCA GGAGCAACAA
    CTCAGAGAGC AGGAGGAATA TAAAAGACAG CTCCTGGCAG AGAGACAGAA GCGCATAGAA
    CAGCAGAAAG AGCAAAGGAG GAGACTTGAA GAGCAACAAA GAAGAGAGCG TGAGGCTCGT
    CGGCAGCAGG AACGAGAACA AAGAAGGCGA GAGCAAGAGG AAAAGAAGAG AATGGAGGAG
    ATGGAAAGAA GGCGCAAGGA AGAGGAAGAA AGGAGAAGAG CAGAGGAAGA GAAAAGAAGA
    GTGGAAAGAG AGCAGGAGTA TAGATGGCGT GAAATGGAGG AGCACCGCCA GGCTGAAAGA
    CTTCAGCGTC AGTTGCAGCA AGAGCAGGCC TATCTCATGT CACTACAGCA TGATCATAGA
    CAGCAGCAGC CACCACAACA GCAGCCCCAG CCCCAGCAGC CTCCACTGTC CCAGCAGGAA
    AGGAGCAAGC AAACATACCA TACCCAAGAA CCCAAGCCTC ATTATGAACA AGCAGAACGA
    ATACGAGAGG CAGAGGAGCG CCTTAGGAAA GCAAACCAAG GCTCCCCTGA AGCCCAGGCT
    AAGCAACCAG GAAGAGTTTT AGAGCCATTA GTGCCTTCAA GATCCGAATC ATTTTCCAAT
    GGAAATTCTG AGCCTGCTCA ACCTGCCATG CACAGACCAG TGGAACCACA GGTACAGTGG
    TCTCATCTGG CATCCCTCAA GAACAATGTA TCCCCTGTCT CGCGATCCCA TTCCTTCAGT
    GACCCTTCTC CCAAATTTGC ACCTCACCTT CTACGATCTC AGGATTCGCA CCAAACTTCA
    CGCAGTGAAG TGCAAAGTCA GGGTTCTGAC CCTAAGTCTG AAATCTCTGA GCCAACCCAA
    AGGGTTTGGG CTCGATCAGA CAGTGACGAG GTGCCTCCAA GGGTGCCTGT GAGGACAACA
    TCAAGATCTC CAGTATTGTC ACGGAAAGAT TCACCACTGC AGGGCAGTGG GCAGCAAAAT
    ACCCAAGCTG GTCAGAGAAA TTCCACAAGC AATATTGAGC CTAGACTTCT TTGGGAAAGA
    GTAGAGAAGC TTGTGCCCAG ACCGGGTAGT GGTAGCTCGT CAGGATCAAG TAACTCTGGA
    TCCCAAGCAG GATCCCACCC AGGATCTCAG AGTGGATCTG GAGAGCGCTT CCGGATGAGA
    TCATCTTCAA AATCTGAGGG CTCACCATCT CAACGACATG AAAACACAGC CAAAAAGTCT
    GAAGAGAAGA AAGAAGCAGT TAGACAGGCC AAAGCTCCTG GAGAAGTGGA TCTTACTGCA
    CTCGCTAAGG AGCTTCGAGC AGTAGATGAT GTTCGGCCAC TTCACAAAGT TACTGACTAT
    TCCTCATCCA GTGAAGAGTC TGGTACTACA GATGAGGAGG ATGATGACAT GGAACAGGAA
    GGAGCAGATG AATCGACTTC AGGCCCAGAG GATGCTAGAG CTATGTCATC TTTGAATTTA
    AGCAATGGAG AAACGGAGTC TGTGAAAACC ATGATCGTAC ATGATGATGT CGAAAGTGAA
    CCTGCCATGA CCCCATCTAA AGAAGGCACT TTAATTGTCC GACAGAGTAC AGTTGACAAA
    AAGCGTGCCA ACCAACTTGA GAGCAATGGC TTTGCGAGTC GTATTCACCT CTTGCCAGAT
    CTCTTACAGC AAAGCCATTC CTCCTCCACC ACTTCTTCCC CAACCTCAAG TCAACCGACA
    CCTACCATGT CCCCCCAGAC ACCCCAGGAC AAGCTAACTG CTAATGAGAC TCAGTCAGCT
    AGTAATACTC TCCAGAAACA CAAATCGTCC TCCTCTTTCA CACCGTTCAT AGATCCAAGG
    TTACTTCAGA TCTCTCCATC TAGTGGAACA ACTGTTACGT CAGTAGGTAC ATACATGCGC
    ACACTGGAAG CTAACCTTAA AAATACTAAT TTTATTTTAG TGGGATTTTC CAGTGAAGCG
    CTAAGGCCGG ATGCCATGAG GCAAGATCCT ACAAGGAAAG GATCAGTTGT TAATGTTAAT
    CCCACAAATA CTCGGCCACA AAGTGATACA CCAGAGATTC GCAAATACAA GAAGAGATTT
    AATTCTGAGA TTTTGTGTGC GGCATTGTGG GGTGTAAATT TGCTGGTGGG AACTGAAAGT
    GGTTTAATGC TGTTGGATAG AAGTGGACAA GGGAAAGTGT ACCCCCTCAT TAACAGAAGG
    CGATTTCAGC AGATGGATGT GCTTGAAGGC TTGAATGTCT TGGTAACTAT ATCAGGGAAA
    AAGAACAAGC TGAGAGTATA TTACTTATCC TGGCTGAGGA ATAAAATTTT GCACAATGAT
    CCTGAAGTAG AGAAGAAACA AGGATGGACA ACAGTTGGGG ATTTGGAAGG CTGTGTGCAC
    TACAAAGTTG TAAAATATGA ACGTATCAAG TTTTTGGTAA TCGCTTTAAA AAGTTCAGTG
    GAGGTGTATG CCTGGGCACC GAAACCATAC CACAAGTTTA TGGCCTTTAA GTCATTCGGA
    GATTTAGTAC ACAGACCTCT CCTTGTAGAC CTGACTGTGG AAGAAGGACA GAGGCTGAAA
    GTGATCTATG GTTCCTGTGC TGGGTTTCAT GCAGTAGATG TGGACTCGGG CGCAGTTTAT
    GATATTTACT TGCCAACACA TATTCAGAGC AGCATTCAGC CACACGCAAT CATCATTCTC
    CCAAATACTG ATGGTATGGA ACTGCTGGTT TGCTATGAAG ATGAAGGAGT TTATGTTAAT
    ACATACGGCA GGATCACCAA AGATGTAGTT CTACAATGGG GAGAAATGCC TACATCAGTT
    GCTTATATCC GATCTAACCA GATCATGGGA TGGGGTGAAA AAGCTATTGA GATTCGATCC
    GTGGAAACTG GCCACCTTGA TGGAGTATTT ATGCACAAAA GGGCACAAAG GCTCAAGTTC
    TTGTGCGAAC GAAATGACAA GGTTTTCTTT GCCTCGGTGC GGTCTGGCGG AAGCAGCCAA
    GTGTATTTCA TGACTTTGGG CAGAAGCTCT CTTCTGAGCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa05833
    Clone ID Related Accession (Same CDS sequence) XM_004911785.3 , XM_004911785.4
    Accession Version XM_004911785.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4029bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004911785.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCCAACG ATTCGCCTGC CAAAAGTCTT GTGGATATAG ACCTGTCGTC TCTGAGGGAC 
    CCAGCTGGCA TCTTTGAATT AGTGGAAGTG GTAGGAAATG GCACCTATGG ACAAGTATAT
    AAGGGCCGAC ATGTCAAAAC AGGCCAACTT GCAGCCATCA AAGTCATGGA TGTTACAGAG
    GATGAAGAGG AAGAAATCAA ACTGGAAATC AATATGTTGA AGAAATATTC CCACCACAGA
    AATATAGCTA CTTATTATGG TGCTTTTATT AAGAAAAGTC CTCCTGGGCA TGATGACCAG
    CTTTGGTTGG TGATGGAGTT TTGTGGTGCT GGCTCAATCA CTGACCTGGT GAAGAATACA
    AAGGGGAATA GTTTGAAAGA AGACTGGATT GCCTATATCT CTCGTGAGAT ACTTAGGGGA
    TTGGCCCACC TCCATGCTCA TCATGTGATT CACCGGGATA TCAAAGGACA GAATGTGCTG
    CTAACAGAGA ATGCCGAAGT GAAATTAGTG GATTTTGGTG TTAGTGCACA GCTGGATAGG
    ACAGTGGGCC GAAGGAACAC ATTTATTGGT ACACCATACT GGATGGCTCC AGAAGTTATC
    GCCTGTGATG AGAACCCAGA TGCTACCTAT GACTATAGGA GTGACCTCTG GTCATGTGGT
    ATTACAGCGA TTGAAATGGC AGAAGGTGCT CCTCCTCTTT GCGATATGCA TCCAATGCGA
    GCCCTGTTCC TTATCCCAAG AAATCCTCCA CCACGGCTAA AATCCAAGAA ATGGTCAAAG
    AAGTTCTTCA GCTTTATAGA AGGCTGCCTT GTGAAAAATT ACATGCAACG TCCCCCAACA
    GAACAGCTTT TAAAGCATCC ATTTATACGA GATCAGCCAA ATGAAAGGCA AGTCCGAATT
    CAGCTGAAGG ATCACATAGA CAGGACAAGG AAGAAGAGAG GAGAAAAAGA TGAAACAGAG
    TATGAATACA GTGGAAGTGA AGAGGAGGAA GAGGAAGTGC CTGAACAGGA AGGAGAACCA
    AGTTCCATTG TTAATGTGCC TGGGGAATCA ACACTCCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGCTC TGAGGCTTTG CGACGGCAAC AGCTGCTGCA GGAGCAACAA
    CTCAGAGAGC AGGAGGAATA TAAAAGACAG CTCCTGGCAG AGAGACAGAA GCGCATAGAA
    CAGCAGAAAG AGCAAAGGAG GAGACTTGAA GAGCAACAAA GAAGAGAGCG TGAGGCTCGT
    CGGCAGCAGG AACGAGAACA AAGAAGGCGA GAGCAAGAGG AAAAGAAGAG AATGGAGGAG
    ATGGAAAGAA GGCGCAAGGA AGAGGAAGAA AGGAGAAGAG CAGAGGAAGA GAAAAGAAGA
    GTGGAAAGAG AGCAGGAGTA TATCCGAAGA CAGCTGGAGG AGGAGCAGCG GCACTTAGAA
    ATCCTTCAGC AGCAGCTGCT TCAGGAGCAG GCCATGTTAC TGGAGTATAG ATGGCGTGAA
    ATGGAGGAGC ACCGCCAGGC TGAAAGACTT CAGCGTCAGT TGCAGCAAGA GCAGGCCTAT
    CTCATGTCAC TACAGCATGA TCATAGACAG CAGCAGCCAC CACAACAGCA GCCCCAGCCC
    CAGCAGCCTC CACTGTCCCA GCAGGAAAGG AGCAAGCAAA CATACCATAC CCAAGAACCC
    AAGCCTCATT ATGAACAAGC AGAACGAATA CGAGAGGCAG AGGAGCGCCT TAGGAAAGCA
    AACCAAGGCT CCCCTGAAGC CCAGGCTAAG CAACCAGGAA GAGTTTTAGA GCCATTAGTG
    CCTTCAAGAT CCGAATCATT TTCCAATGGA AATTCTGAGC CTGCTCAACC TGCCATGCAC
    AGACCAGTGG AACCACAGGT ACAGTGGTCT CATCTGGCAT CCCTCAAGAA CAATGTATCC
    CCTGTCTCGC GATCCCATTC CTTCAGTGAC CCTTCTCCCA AATTTGCACC TCACCTTCTA
    CGATCTCAGG ATTCGCACCA AACTTCACGC AGTGAAGTGC AAAGTCAGGG TTCTGACCCT
    AAGTCTGAAA TCTCTGAGCC AACCCAAAGG GTTTGGGCTC GATCAGACAG TGACGAGGTG
    CCTCCAAGGG TGCCTGTGAG GACAACATCA AGATCTCCAG TATTGTCACG GAAAGATTCA
    CCACTGCAGG GCAGTGGGCA GCAAAATACC CAAGCTGGTC AGAGAAATTC CACAAGCAAT
    ATTGAGCCTA GACTTCTTTG GGAAAGAGTA GAGAAGCTTG TGCCCAGACC GGGTAGTGGT
    AGCTCGTCAG GATCAAGTAA CTCTGGATCC CAAGCAGGAT CCCACCCAGG ATCTCAGAGT
    GGATCTGGAG AGCGCTTCCG GATGAGATCA TCTTCAAAAT CTGAGGGCTC ACCATCTCAA
    CGACATGAAA ACACAGCCAA AAAGTCTGAA GAGAAGAAAG AAGCAGTTAG ACAGGCCAAA
    GCTCCTGGAG AAGTGGATCT TACTGCACTC GCTAAGGAGC TTCGAGCAGT AGATGATGTT
    CGGCCACTTC ACAAAGTTAC TGACTATTCC TCATCCAGTG AAGAGTCTGG TACTACAGAT
    GAGGAGGATG ATGACATGGA ACAGGAAGGA GCAGATGAAT CGACTTCAGG CCCAGAGGAT
    GCTAGAGCTA TGTCATCTTT GAATTTAAGC AATGGAGAAA CGGAGTCTGT GAAAACCATG
    ATCGTACATG ATGATGTCGA AAGTGAACCT GCCATGACCC CATCTAAAGA AGGCACTTTA
    ATTGTCCGAC AGACTCAGTC AGCTAGTAAT ACTCTCCAGA AACACAAATC GTCCTCCTCT
    TTCACACCGT TCATAGATCC AAGGTTACTT CAGATCTCTC CATCTAGTGG AACAACTGTT
    ACGTCAGTAG GTACATACAT GCGCACACTG GAAGCTAACC TTAAAAATAC TAATTTTATT
    TTAGTGGGAT TTTCCAGTGA AGCGCTAAGG CCGGATGCCA TGAGGCAAGA TCCTACAAGG
    AAAGGATCAG TTGTTAATGT TAATCCCACA AATACTCGGC CACAAAGTGA TACACCAGAG
    ATTCGCAAAT ACAAGAAGAG ATTTAATTCT GAGATTTTGT GTGCGGCATT GTGGGGTGTA
    AATTTGCTGG TGGGAACTGA AAGTGGTTTA ATGCTGTTGG ATAGAAGTGG ACAAGGGAAA
    GTGTACCCCC TCATTAACAG AAGGCGATTT CAGCAGATGG ATGTGCTTGA AGGCTTGAAT
    GTCTTGGTAA CTATATCAGG GAAAAAGAAC AAGCTGAGAG TATATTACTT ATCCTGGCTG
    AGGAATAAAA TTTTGCACAA TGATCCTGAA GTAGAGAAGA AACAAGGATG GACAACAGTT
    GGGGATTTGG AAGGCTGTGT GCACTACAAA GTTGTAAAAT ATGAACGTAT CAAGTTTTTG
    GTAATCGCTT TAAAAAGTTC AGTGGAGGTG TATGCCTGGG CACCGAAACC ATACCACAAG
    TTTATGGCCT TTAAGTCATT CGGAGATTTA GTACACAGAC CTCTCCTTGT AGACCTGACT
    GTGGAAGAAG GACAGAGGCT GAAAGTGATC TATGGTTCCT GTGCTGGGTT TCATGCAGTA
    GATGTGGACT CGGGCGCAGT TTATGATATT TACTTGCCAA CACATATTCA GAGCAGCATT
    CAGCCACACG CAATCATCAT TCTCCCAAAT ACTGATGGTA TGGAACTGCT GGTTTGCTAT
    GAAGATGAAG GAGTTTATGT TAATACATAC GGCAGGATCA CCAAAGATGT AGTTCTACAA
    TGGGGAGAAA TGCCTACATC AGTTGCTTAT ATCCGATCTA ACCAGATCAT GGGATGGGGT
    GAAAAAGCTA TTGAGATTCG ATCCGTGGAA ACTGGCCACC TTGATGGAGT ATTTATGCAC
    AAAAGGGCAC AAAGGCTCAA GTTCTTGTGC GAACGAAATG ACAAGGTTTT CTTTGCCTCG
    GTGCGGTCTG GCGGAAGCAG CCAAGTGTAT TTCATGACTT TGGGCAGAAG CTCTCTTCTG
    AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004911842.1
    CDS348..4376
    Translation

    Target ORF information:

    RefSeq Version XM_004911785.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 4 (map4k4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004911785.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCCAACG ATTCGCCTGC CAAAAGTCTT GTGGATATAG ACCTGTCGTC TCTGAGGGAC 
    CCAGCTGGCA TCTTTGAATT AGTGGAAGTG GTAGGAAATG GCACCTATGG ACAAGTATAT
    AAGGGCCGAC ATGTCAAAAC AGGCCAACTT GCAGCCATCA AAGTCATGGA TGTTACAGAG
    GATGAAGAGG AAGAAATCAA ACTGGAAATC AATATGTTGA AGAAATATTC CCACCACAGA
    AATATAGCTA CTTATTATGG TGCTTTTATT AAGAAAAGTC CTCCTGGGCA TGATGACCAG
    CTTTGGTTGG TGATGGAGTT TTGTGGTGCT GGCTCAATCA CTGACCTGGT GAAGAATACA
    AAGGGGAATA GTTTGAAAGA AGACTGGATT GCCTATATCT CTCGTGAGAT ACTTAGGGGA
    TTGGCCCACC TCCATGCTCA TCATGTGATT CACCGGGATA TCAAAGGACA GAATGTGCTG
    CTAACAGAGA ATGCCGAAGT GAAATTAGTG GATTTTGGTG TTAGTGCACA GCTGGATAGG
    ACAGTGGGCC GAAGGAACAC ATTTATTGGT ACACCATACT GGATGGCTCC AGAAGTTATC
    GCCTGTGATG AGAACCCAGA TGCTACCTAT GACTATAGGA GTGACCTCTG GTCATGTGGT
    ATTACAGCGA TTGAAATGGC AGAAGGTGCT CCTCCTCTTT GCGATATGCA TCCAATGCGA
    GCCCTGTTCC TTATCCCAAG AAATCCTCCA CCACGGCTAA AATCCAAGAA ATGGTCAAAG
    AAGTTCTTCA GCTTTATAGA AGGCTGCCTT GTGAAAAATT ACATGCAACG TCCCCCAACA
    GAACAGCTTT TAAAGCATCC ATTTATACGA GATCAGCCAA ATGAAAGGCA AGTCCGAATT
    CAGCTGAAGG ATCACATAGA CAGGACAAGG AAGAAGAGAG GAGAAAAAGA TGAAACAGAG
    TATGAATACA GTGGAAGTGA AGAGGAGGAA GAGGAAGTGC CTGAACAGGA AGGAGAACCA
    AGTTCCATTG TTAATGTGCC TGGGGAATCA ACACTCCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGCTC TGAGGCTTTG CGACGGCAAC AGCTGCTGCA GGAGCAACAA
    CTCAGAGAGC AGGAGGAATA TAAAAGACAG CTCCTGGCAG AGAGACAGAA GCGCATAGAA
    CAGCAGAAAG AGCAAAGGAG GAGACTTGAA GAGCAACAAA GAAGAGAGCG TGAGGCTCGT
    CGGCAGCAGG AACGAGAACA AAGAAGGCGA GAGCAAGAGG AAAAGAAGAG AATGGAGGAG
    ATGGAAAGAA GGCGCAAGGA AGAGGAAGAA AGGAGAAGAG CAGAGGAAGA GAAAAGAAGA
    GTGGAAAGAG AGCAGGAGTA TATCCGAAGA CAGCTGGAGG AGGAGCAGCG GCACTTAGAA
    ATCCTTCAGC AGCAGCTGCT TCAGGAGCAG GCCATGTTAC TGGAGTATAG ATGGCGTGAA
    ATGGAGGAGC ACCGCCAGGC TGAAAGACTT CAGCGTCAGT TGCAGCAAGA GCAGGCCTAT
    CTCATGTCAC TACAGCATGA TCATAGACAG CAGCAGCCAC CACAACAGCA GCCCCAGCCC
    CAGCAGCCTC CACTGTCCCA GCAGGAAAGG AGCAAGCAAA CATACCATAC CCAAGAACCC
    AAGCCTCATT ATGAACAAGC AGAACGAATA CGAGAGGCAG AGGAGCGCCT TAGGAAAGCA
    AACCAAGGCT CCCCTGAAGC CCAGGCTAAG CAACCAGGAA GAGTTTTAGA GCCATTAGTG
    CCTTCAAGAT CCGAATCATT TTCCAATGGA AATTCTGAGC CTGCTCAACC TGCCATGCAC
    AGACCAGTGG AACCACAGGT ACAGTGGTCT CATCTGGCAT CCCTCAAGAA CAATGTATCC
    CCTGTCTCGC GATCCCATTC CTTCAGTGAC CCTTCTCCCA AATTTGCACC TCACCTTCTA
    CGATCTCAGG ATTCGCACCA AACTTCACGC AGTGAAGTGC AAAGTCAGGG TTCTGACCCT
    AAGTCTGAAA TCTCTGAGCC AACCCAAAGG GTTTGGGCTC GATCAGACAG TGACGAGGTG
    CCTCCAAGGG TGCCTGTGAG GACAACATCA AGATCTCCAG TATTGTCACG GAAAGATTCA
    CCACTGCAGG GCAGTGGGCA GCAAAATACC CAAGCTGGTC AGAGAAATTC CACAAGCAAT
    ATTGAGCCTA GACTTCTTTG GGAAAGAGTA GAGAAGCTTG TGCCCAGACC GGGTAGTGGT
    AGCTCGTCAG GATCAAGTAA CTCTGGATCC CAAGCAGGAT CCCACCCAGG ATCTCAGAGT
    GGATCTGGAG AGCGCTTCCG GATGAGATCA TCTTCAAAAT CTGAGGGCTC ACCATCTCAA
    CGACATGAAA ACACAGCCAA AAAGTCTGAA GAGAAGAAAG AAGCAGTTAG ACAGGCCAAA
    GCTCCTGGAG AAGTGGATCT TACTGCACTC GCTAAGGAGC TTCGAGCAGT AGATGATGTT
    CGGCCACTTC ACAAAGTTAC TGACTATTCC TCATCCAGTG AAGAGTCTGG TACTACAGAT
    GAGGAGGATG ATGACATGGA ACAGGAAGGA GCAGATGAAT CGACTTCAGG CCCAGAGGAT
    GCTAGAGCTA TGTCATCTTT GAATTTAAGC AATGGAGAAA CGGAGTCTGT GAAAACCATG
    ATCGTACATG ATGATGTCGA AAGTGAACCT GCCATGACCC CATCTAAAGA AGGCACTTTA
    ATTGTCCGAC AGACTCAGTC AGCTAGTAAT ACTCTCCAGA AACACAAATC GTCCTCCTCT
    TTCACACCGT TCATAGATCC AAGGTTACTT CAGATCTCTC CATCTAGTGG AACAACTGTT
    ACGTCAGTAG GTACATACAT GCGCACACTG GAAGCTAACC TTAAAAATAC TAATTTTATT
    TTAGTGGGAT TTTCCAGTGA AGCGCTAAGG CCGGATGCCA TGAGGCAAGA TCCTACAAGG
    AAAGGATCAG TTGTTAATGT TAATCCCACA AATACTCGGC CACAAAGTGA TACACCAGAG
    ATTCGCAAAT ACAAGAAGAG ATTTAATTCT GAGATTTTGT GTGCGGCATT GTGGGGTGTA
    AATTTGCTGG TGGGAACTGA AAGTGGTTTA ATGCTGTTGG ATAGAAGTGG ACAAGGGAAA
    GTGTACCCCC TCATTAACAG AAGGCGATTT CAGCAGATGG ATGTGCTTGA AGGCTTGAAT
    GTCTTGGTAA CTATATCAGG GAAAAAGAAC AAGCTGAGAG TATATTACTT ATCCTGGCTG
    AGGAATAAAA TTTTGCACAA TGATCCTGAA GTAGAGAAGA AACAAGGATG GACAACAGTT
    GGGGATTTGG AAGGCTGTGT GCACTACAAA GTTGTAAAAT ATGAACGTAT CAAGTTTTTG
    GTAATCGCTT TAAAAAGTTC AGTGGAGGTG TATGCCTGGG CACCGAAACC ATACCACAAG
    TTTATGGCCT TTAAGTCATT CGGAGATTTA GTACACAGAC CTCTCCTTGT AGACCTGACT
    GTGGAAGAAG GACAGAGGCT GAAAGTGATC TATGGTTCCT GTGCTGGGTT TCATGCAGTA
    GATGTGGACT CGGGCGCAGT TTATGATATT TACTTGCCAA CACATATTCA GAGCAGCATT
    CAGCCACACG CAATCATCAT TCTCCCAAAT ACTGATGGTA TGGAACTGCT GGTTTGCTAT
    GAAGATGAAG GAGTTTATGT TAATACATAC GGCAGGATCA CCAAAGATGT AGTTCTACAA
    TGGGGAGAAA TGCCTACATC AGTTGCTTAT ATCCGATCTA ACCAGATCAT GGGATGGGGT
    GAAAAAGCTA TTGAGATTCG ATCCGTGGAA ACTGGCCACC TTGATGGAGT ATTTATGCAC
    AAAAGGGCAC AAAGGCTCAA GTTCTTGTGC GAACGAAATG ACAAGGTTTT CTTTGCCTCG
    GTGCGGTCTG GCGGAAGCAG CCAAGTGTAT TTCATGACTT TGGGCAGAAG CTCTCTTCTG
    AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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