scn4a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	scn4a
Entrez Gene ID	100492371
Full Name	sodium channel, voltage gated, type IV alpha subunit
Synonyms	na(v)1.4,nav1.4
General protein information	Preferred Names sodium channel, voltage gated, type IV alpha subunit Names sodium channel protein type 4 subunit alpha
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018089322.1	XP_017944811.1	sodium channel protein type 4 subunit alpha
XM_031894528.1	XP_031750388.1	sodium channel protein type 4 subunit alpha isoform X2
XM_018089322.2	XP_017944811.2	sodium channel protein type 4 subunit alpha isoform X1
XM_031894526.1	XP_031750386.1	sodium channel protein type 4 subunit alpha isoform X1
XM_031894527.1	XP_031750387.1	sodium channel protein type 4 subunit alpha isoform X1
XM_031894530.1	XP_031750390.1	sodium channel protein type 4 subunit alpha isoform X3
XM_031894531.1	XP_031750391.1	sodium channel protein type 4 subunit alpha isoform X4
XM_031894529.1	XP_031750389.1	sodium channel protein type 4 subunit alpha isoform X3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SCN4A	XP_853396.1
Rattus norvegicus (Norway rat)	Scn4a	NP_037310.1
Pan troglodytes (chimpanzee)	SCN4A	XP_003315741.1
	SCN4A	XP_001116451.1
Mus musculus (house mouse)	Scn4a	NP_573462.2
Xenopus tropicalis (tropical clawed frog)	LOC100492371	XP_002933087.2
Homo sapiens (human)	SCN4A	NP_000325.4
Bos taurus (cattle)	SCN4A	XP_003587508.1
Danio rerio (zebrafish)	scn4aa	NP_001034914.1
Gallus gallus (chicken)	SCN4A	XP_004948566.1

Gene Ontology

The following scn4a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the scn4a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa50621	XM_031894531.1 Latest version!	Xenopus tropicalis sodium channel, voltage gated, type IV alpha subunit (scn4a), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa50618	XM_031894527.1 Latest version!	Xenopus tropicalis sodium channel, voltage gated, type IV alpha subunit (scn4a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa50618	XM_018089322.2 Latest version!	Xenopus tropicalis sodium channel, voltage gated, type IV alpha subunit (scn4a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa50618	XM_031894526.1 Latest version!	Xenopus tropicalis sodium channel, voltage gated, type IV alpha subunit (scn4a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa50619	XM_031894528.1 Latest version!	Xenopus tropicalis sodium channel, voltage gated, type IV alpha subunit (scn4a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa50620	XM_031894529.1 Latest version!	Xenopus tropicalis sodium channel, voltage gated, type IV alpha subunit (scn4a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa50620	XM_031894530.1 Latest version!	Xenopus tropicalis sodium channel, voltage gated, type IV alpha subunit (scn4a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa35367	XM_018089322.1 Latest version!	Xenopus tropicalis sodium channel, voltage gated, type IV alpha subunit (scn4a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

scn4a ( XM_031894531.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031750391.1 Xenopus tropicalis sodium channel, voltage gated, type IV alpha subunit (scn4a), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OXa50621
Clone ID Related Accession (Same CDS sequence)	XM_031894531.1
Accession Version	XM_031894531.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sodium channel protein type 4 subunit alpha isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGCA AGGTGCACCC TCCCGGTCCT GATAGTTTGC GTCCATTTAC CAGAGAGTCC 
TTGGCTGCGA TAGAGAAGCG CATTGAGGAG GACATCGCTC GGAAAGCCAA ACAGATTGAG 
GTGCTCGATG AGAAAGTACC GACACCCAAA AATGGACTGG AGGCAGGAAA GAACTTGCCT 
CTTATATATG GGGATCCCCC ACCCGAACTT ATTGGGATTC CCATAGAGGA ACTAGATTCT 
TTTTACAGCA ACCAAAAGAC ATTTATCGTC TTAAACAAAG GAAAAGCAAT ATTCCGATTC 
ACAGCCACCA AAGCCTTATA TCTACTGGAT CCATTTAATC CAATCAGAAG AGGCGCTATT 
AAAGTCTTAA TCCATTCATT GTTTAGCTTA TTTATTATGT TTACCATCTT AACAAATTGT 
GTTTTTATGA CCATGAGTGA CCCACCACCC TGGTCCAAGA ATGTAGAGTA CACGTTTACT 
GGAATTTACA CCTTTGAGTC CTTAATAAAA ATCCTGGCTC GGGGTTTTTG TATTGACAGT 
TTTACATTCC TCAGGGATCC GTGGAACTGG CTGGATTTCT GTGTCATTGT TATGGCTTAC 
ACCACAGAGT TTGTAGATTT GGGGAATGTG TCTGCCCTTA GGACATTCCG TGTACTCCGT 
GCTCTTAAAA CAATTACAGT TATTCCAGGC CTGAAGACTA TCGTTGGTGC CTTGATCCAA 
TCAGTGAAGA AGCTCGCAGA TGTGATGATC CTCACCCTCT TCTGCCTTGC TGTCTTTGCC 
CTCATTGGCC TTCAGCTCTT TATGGGAAAT CTGAGGCACA AATGTGTCCG ATGGCCACCC 
CCTGATGACT ATTTCATGCT TTATGACAAC AGTACCTTAA ACTCCACACT ATATAATGAT 
ACAATGATCA ACTCCACTCT CAGCAACAGC ACAAATGTGT TCGACAACGA CACCATTGAC 
TGGGGTGCCT ATCTCAACGA TGAAGCAAAT TTTTACTTCC TGGATGGAGC AAATGATGCA 
CTTCTGTGTG GGAACAGCAG TGATGCCGGG AAATGTCCAG AAAGTTACAC ATGTATGAAA 
ACTGGGAGAA ATCCGAATTA TGGGTACACC AGCTACGACT CATTCAACTG GGCTTTCCTT 
TCCCTTTTCC GATTAATGAC ACAGGACTTC TGGGAGAATC TTTTCCAGTT GACACTACGA 
GCAGCTGGTA AAACCTACAT GGTCTTCTTT GTGGTGGTAA TTTTTCTGGG GTCTTTCTAT 
CTGATCAACT TGATCCTTGC TGTGGTGGCC ATGGCATATG CAGAACAGAA TGAAGCCACT 
CTGCAAGAAG AAATAGAAAA GGAGAAAGAG TTCCAGGAGC TTTTGTTGCA GCTAAAGAAA 
CATCAAGAAG AGCAAGCAAG AAGGAAATCC AGCGTTGCCT CAGTTGGACT TCTGAACGCA 
GAAAAGAAAA ATGCTCTTTC AGAGAACACT CTTTCTGGGG ATGGCACAGA AAATATGGAC 
TGCAATGGGC GAACTGTGCC CAAAGTTATA ATTGAACGTG CACCCTCAAT GGAGGATACA 
AGCTCAGCAC AGTCAGACCA ACAGAGGAAT TCTAAAGTTT ATTTGGAGGA GCCAACTCTG 
CAGAAAAGAG CAGAGAGCAC CCACAGTGTC CTATCGGAAG TAGCTCTTGA AGAACTGGAA 
GAGGCTCATC AGAAATGCCC TCCTTGGTGG TACAAGTTTG CTAACACCTT TTTGATTTGG 
GACTGCTGTG ACCTGTGGGT GAAATTTAAG GAATTTGTAA GGTTTGTGGT CATGGATCCC 
TTTGTTGACC TGGGAATCAC CATTTGCATC GTGCTTAACA CCCTTTTTAT GGCCATGGAG 
CACTACCCAA TGACTGAGCA GTTTGAGGGT GTTCTGAATG TAGGAAACTT GGTCTTTACT 
GGCATATTTG CAGCTGAAAT GTTCTTCAAG ATCATAGCCC TGGATCCATA TTACTACTTC 
CAAGTGGGGT GGAACATATT CGACAGCATT ATAGTCACTT TAAGTTTGGT GGAGTTGGGC 
CTTGCAAATG TGGAAGGTCT GTCCGTGCTC AGATCGTTCC GTCTGCTCAG GGTGTTCAAG 
CTAGCCAAAT CATGGCCAAC CCTTAACATG CTTATCAAAA TCATCGGTAA CTCGGTCGGA 
GCACTTGGAA ACCTCACCTT GGTGTTGGCC ATCATTGTCT TCATCTTTGC GGTGGTGGGG 
ATGCAGCTAT TTGGGAAGAG CTACAAGGAA TGTGTGTGCA AGATTGCCTC TGACTGCGAG 
CTACCACGGT GGCACATGCA CGATTTCTTC CACTCTTTTC TCATTGTCTT CCGTATCCTG 
TGCGGGGAGT GGATAGAGAC AATGTGGGAC TGCATGGAAG TGGCAGGGCA AGCAATGTGC 
CTGGTCGTGT TCATGATGGT CATGGTGATT GGTAACCTGG TGGTCCTCAA TCTCTTTTTG 
GCTTTGTTGC TGAGTTCCTT CAGCGCGGAT AACTTGTCGG CTTCGGACGA CGATGGGGAG 
ATGAACAACC TGCAGATCGC CATCGGAAGG ATCACCAGGG GCATTGACTT TGTGAAGAAA 
ACCTTCTTCT CCATCCTACA TTACAAGAGG AAGGCAAAAG CTGAGCAGGA GGAAGAGGAC 
CCTAACAAAG AAAACATTGC TCTGAACCAT GTAGAGACAG AACCAGAACC CAAGATAGAA 
CTAGAACCCA GGACAGAACC AGAACCCAAA AAAGAACCTC CACTGATTGA TGGCGTGACT 
AAAAAACGTT ATTACATGGA TGAGTTTGAC CACGCTAATT TTTTTAACAA CCCACACATG 
AGAATAGAAG TACCTATTGC CTCAGAAGAG TCCGACTTCG ACATTCCAGA CACGGATGAG 
TTTAGCACAC AGTCGGAGGT AGAGGAAGGG ATTTTACACC ACAAGAAGTA CGATGATGGC 
TCGTCAGTGT GTAGCACAGC AGATTATAAA CCTCCTGAAC CTGAAGAGGA AGAGGAGGCC 
TTTGAACACA AAGAAGACGA AGGCCCAGAA GAATGCTTTA CTGCTGACTG TGTACGAAGA 
TGCCCTTGTC TTTATGTCGA CATCACAACA GAAAAAGGGA AAATTTGGTG GAACATTCGG 
AAAACATGTT TCCAGATTGT GGAGCATAAC TGGTTTGAAA CCTTCATCAT CTTCATGATC 
TTACTCAGTA GTGGAGCACT GGCATTTGAG GATATCTACA TTGAACAGAG GCAAGTGATA 
AAGACTATCC TGGAGTACGC TGATAAAGTC TTCACATATA TTTTTGTGTT TGAAATGCTG 
CTAAAGTGGA CAGCGTATGG CTTCAAGATC TATTTCACGA ATGCCTGGTG CTGGCTGGAC 
TTCCTAATTG TTGACGTGTC TTTAATCAGC CTGACAGCCA ACTGGCTGGG CTACTCGGAA 
CTTGGTCCCA TCAAATCCCT TAGAACGCTG AGGGCCCTTA GGCCGTTACG AGCGTTATCA 
AGATTTGAAG GCATGAGGGT GGTTGTAAAC GCCCTTCTGG GAGCCATTCC TTCTATCATG 
AATGTCCTGC TTGTTTGTCT GATCTTCTGG CTCATCTTCA GCATCATGGG TGTTAACCTC 
TTTGCTGGGA AATATTATCG ATGCATCAAC ACGACCACGT CAGAATTATT TGAGATCGAT 
GTGGTCAACA ACCGCTCGGA CTGCCTCGCT TTAAACAACT CCGAGCAAGT GCGATGGGTT 
AATGTCAAAG TGAATTTTGA CAATGTGGGC CTTGGGTATC TCTCCCTTCT ACAAGTGGCA 
ACCTTCAAAG GCTGGATGGA TATCATGTAT GCCGCTGTAG ATTCCCGGAA TATTGAGGAT 
CAGCCACAAT ATGAAATAAA TCTTTATATG TATTTGTACT TTGTAATATT CATTATTTTT 
GGAGCTTTCT TCACTCTAAA CCTGTTTATC GGTGTCATTA TCGACAACTT CAATCAGCAA 
AAGAAAAAGT TTGGAGGTAA GGATATCTTT ATGACTGAGG AACAGAAGAA ATACTACAAT 
GCTATGAAAA AGCTGGGATC CAAAAAACCC CAAAAGCCAA TTCCAAGACC CACGGTATTG 
TTCTCTCGGA TCTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031750391.1
CDS	159..4256
Translation	MAGKVHPPGPDSLRPFTRESLAAIEKRIEEDIARKAKQIEVLDEKVPTPKNGLEAGKNLPLIYGDPPPELIGIPIEELDSFYSNQKTFIVLNKGKAIFRFTATKALYLLDPFNPIRRGAIKVLIHSLFSLFIMFTILTNCVFMTMSDPPPWSKNVEYTFTGIYTFESLIKILARGFCIDSFTFLRDPWNWLDFCVIVMAYTTEFVDLGNVSALRTFRVLRALKTITVIPGLKTIVGALIQSVKKLADVMILTLFCLAVFALIGLQLFMGNLRHKCVRWPPPDDYFMLYDNSTLNSTLYNDTMINSTLSNSTNVFDNDTIDWGAYLNDEANFYFLDGANDALLCGNSSDAGKCPESYTCMKTGRNPNYGYTSYDSFNWAFLSLFRLMTQDFWENLFQLTLRAAGKTYMVFFVVVIFLGSFYLINLILAVVAMAYAEQNEATLQEEIEKEKEFQELLLQLKKHQEEQARRKSSVASVGLLNAEKKNALSENTLSGDGTENMDCNGRTVPKVIIERAPSMEDTSSAQSDQQRNSKVYLEEPTLQKRAESTHSVLSEVALEELEEAHQKCPPWWYKFANTFLIWDCCDLWVKFKEFVRFVVMDPFVDLGITICIVLNTLFMAMEHYPMTEQFEGVLNVGNLVFTGIFAAEMFFKIIALDPYYYFQVGWNIFDSIIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKIASDCELPRWHMHDFFHSFLIVFRILCGEWIETMWDCMEVAGQAMCLVVFMMVMVIGNLVVLNLFLALLLSSFSADNLSASDDDGEMNNLQIAIGRITRGIDFVKKTFFSILHYKRKAKAEQEEEDPNKENIALNHVETEPEPKIELEPRTEPEPKKEPPLIDGVTKKRYYMDEFDHANFFNNPHMRIEVPIASEESDFDIPDTDEFSTQSEVEEGILHHKKYDDGSSVCSTADYKPPEPEEEEEAFEHKEDEGPEECFTADCVRRCPCLYVDITTEKGKIWWNIRKTCFQIVEHNWFETFIIFMILLSSGALAFEDIYIEQRQVIKTILEYADKVFTYIFVFEMLLKWTAYGFKIYFTNAWCWLDFLIVDVSLISLTANWLGYSELGPIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKYYRCINTTTSELFEIDVVNNRSDCLALNNSEQVRWVNVKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSRNIEDQPQYEINLYMYLYFVIFIIFGAFFTLNLFIGVIIDNFNQQKKKFGGKDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPTVLFSRIS

Target ORF information:

RefSeq Version	XM_031894531.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sodium channel, voltage gated, type IV alpha subunit (scn4a), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031894531.1

ORF Insert Sequence:

ATGGCTGGCA AGGTGCACCC TCCCGGTCCT GATAGTTTGC GTCCATTTAC CAGAGAGTCC 
TTGGCTGCGA TAGAGAAGCG CATTGAGGAG GACATCGCTC GGAAAGCCAA ACAGATTGAG 
GTGCTCGATG AGAAAGTACC GACACCCAAA AATGGACTGG AGGCAGGAAA GAACTTGCCT 
CTTATATATG GGGATCCCCC ACCCGAACTT ATTGGGATTC CCATAGAGGA ACTAGATTCT 
TTTTACAGCA ACCAAAAGAC ATTTATCGTC TTAAACAAAG GAAAAGCAAT ATTCCGATTC 
ACAGCCACCA AAGCCTTATA TCTACTGGAT CCATTTAATC CAATCAGAAG AGGCGCTATT 
AAAGTCTTAA TCCATTCATT GTTTAGCTTA TTTATTATGT TTACCATCTT AACAAATTGT 
GTTTTTATGA CCATGAGTGA CCCACCACCC TGGTCCAAGA ATGTAGAGTA CACGTTTACT 
GGAATTTACA CCTTTGAGTC CTTAATAAAA ATCCTGGCTC GGGGTTTTTG TATTGACAGT 
TTTACATTCC TCAGGGATCC GTGGAACTGG CTGGATTTCT GTGTCATTGT TATGGCTTAC 
ACCACAGAGT TTGTAGATTT GGGGAATGTG TCTGCCCTTA GGACATTCCG TGTACTCCGT 
GCTCTTAAAA CAATTACAGT TATTCCAGGC CTGAAGACTA TCGTTGGTGC CTTGATCCAA 
TCAGTGAAGA AGCTCGCAGA TGTGATGATC CTCACCCTCT TCTGCCTTGC TGTCTTTGCC 
CTCATTGGCC TTCAGCTCTT TATGGGAAAT CTGAGGCACA AATGTGTCCG ATGGCCACCC 
CCTGATGACT ATTTCATGCT TTATGACAAC AGTACCTTAA ACTCCACACT ATATAATGAT 
ACAATGATCA ACTCCACTCT CAGCAACAGC ACAAATGTGT TCGACAACGA CACCATTGAC 
TGGGGTGCCT ATCTCAACGA TGAAGCAAAT TTTTACTTCC TGGATGGAGC AAATGATGCA 
CTTCTGTGTG GGAACAGCAG TGATGCCGGG AAATGTCCAG AAAGTTACAC ATGTATGAAA 
ACTGGGAGAA ATCCGAATTA TGGGTACACC AGCTACGACT CATTCAACTG GGCTTTCCTT 
TCCCTTTTCC GATTAATGAC ACAGGACTTC TGGGAGAATC TTTTCCAGTT GACACTACGA 
GCAGCTGGTA AAACCTACAT GGTCTTCTTT GTGGTGGTAA TTTTTCTGGG GTCTTTCTAT 
CTGATCAACT TGATCCTTGC TGTGGTGGCC ATGGCATATG CAGAACAGAA TGAAGCCACT 
CTGCAAGAAG AAATAGAAAA GGAGAAAGAG TTCCAGGAGC TTTTGTTGCA GCTAAAGAAA 
CATCAAGAAG AGCAAGCAAG AAGGAAATCC AGCGTTGCCT CAGTTGGACT TCTGAACGCA 
GAAAAGAAAA ATGCTCTTTC AGAGAACACT CTTTCTGGGG ATGGCACAGA AAATATGGAC 
TGCAATGGGC GAACTGTGCC CAAAGTTATA ATTGAACGTG CACCCTCAAT GGAGGATACA 
AGCTCAGCAC AGTCAGACCA ACAGAGGAAT TCTAAAGTTT ATTTGGAGGA GCCAACTCTG 
CAGAAAAGAG CAGAGAGCAC CCACAGTGTC CTATCGGAAG TAGCTCTTGA AGAACTGGAA 
GAGGCTCATC AGAAATGCCC TCCTTGGTGG TACAAGTTTG CTAACACCTT TTTGATTTGG 
GACTGCTGTG ACCTGTGGGT GAAATTTAAG GAATTTGTAA GGTTTGTGGT CATGGATCCC 
TTTGTTGACC TGGGAATCAC CATTTGCATC GTGCTTAACA CCCTTTTTAT GGCCATGGAG 
CACTACCCAA TGACTGAGCA GTTTGAGGGT GTTCTGAATG TAGGAAACTT GGTCTTTACT 
GGCATATTTG CAGCTGAAAT GTTCTTCAAG ATCATAGCCC TGGATCCATA TTACTACTTC 
CAAGTGGGGT GGAACATATT CGACAGCATT ATAGTCACTT TAAGTTTGGT GGAGTTGGGC 
CTTGCAAATG TGGAAGGTCT GTCCGTGCTC AGATCGTTCC GTCTGCTCAG GGTGTTCAAG 
CTAGCCAAAT CATGGCCAAC CCTTAACATG CTTATCAAAA TCATCGGTAA CTCGGTCGGA 
GCACTTGGAA ACCTCACCTT GGTGTTGGCC ATCATTGTCT TCATCTTTGC GGTGGTGGGG 
ATGCAGCTAT TTGGGAAGAG CTACAAGGAA TGTGTGTGCA AGATTGCCTC TGACTGCGAG 
CTACCACGGT GGCACATGCA CGATTTCTTC CACTCTTTTC TCATTGTCTT CCGTATCCTG 
TGCGGGGAGT GGATAGAGAC AATGTGGGAC TGCATGGAAG TGGCAGGGCA AGCAATGTGC 
CTGGTCGTGT TCATGATGGT CATGGTGATT GGTAACCTGG TGGTCCTCAA TCTCTTTTTG 
GCTTTGTTGC TGAGTTCCTT CAGCGCGGAT AACTTGTCGG CTTCGGACGA CGATGGGGAG 
ATGAACAACC TGCAGATCGC CATCGGAAGG ATCACCAGGG GCATTGACTT TGTGAAGAAA 
ACCTTCTTCT CCATCCTACA TTACAAGAGG AAGGCAAAAG CTGAGCAGGA GGAAGAGGAC 
CCTAACAAAG AAAACATTGC TCTGAACCAT GTAGAGACAG AACCAGAACC CAAGATAGAA 
CTAGAACCCA GGACAGAACC AGAACCCAAA AAAGAACCTC CACTGATTGA TGGCGTGACT 
AAAAAACGTT ATTACATGGA TGAGTTTGAC CACGCTAATT TTTTTAACAA CCCACACATG 
AGAATAGAAG TACCTATTGC CTCAGAAGAG TCCGACTTCG ACATTCCAGA CACGGATGAG 
TTTAGCACAC AGTCGGAGGT AGAGGAAGGG ATTTTACACC ACAAGAAGTA CGATGATGGC 
TCGTCAGTGT GTAGCACAGC AGATTATAAA CCTCCTGAAC CTGAAGAGGA AGAGGAGGCC 
TTTGAACACA AAGAAGACGA AGGCCCAGAA GAATGCTTTA CTGCTGACTG TGTACGAAGA 
TGCCCTTGTC TTTATGTCGA CATCACAACA GAAAAAGGGA AAATTTGGTG GAACATTCGG 
AAAACATGTT TCCAGATTGT GGAGCATAAC TGGTTTGAAA CCTTCATCAT CTTCATGATC 
TTACTCAGTA GTGGAGCACT GGCATTTGAG GATATCTACA TTGAACAGAG GCAAGTGATA 
AAGACTATCC TGGAGTACGC TGATAAAGTC TTCACATATA TTTTTGTGTT TGAAATGCTG 
CTAAAGTGGA CAGCGTATGG CTTCAAGATC TATTTCACGA ATGCCTGGTG CTGGCTGGAC 
TTCCTAATTG TTGACGTGTC TTTAATCAGC CTGACAGCCA ACTGGCTGGG CTACTCGGAA 
CTTGGTCCCA TCAAATCCCT TAGAACGCTG AGGGCCCTTA GGCCGTTACG AGCGTTATCA 
AGATTTGAAG GCATGAGGGT GGTTGTAAAC GCCCTTCTGG GAGCCATTCC TTCTATCATG 
AATGTCCTGC TTGTTTGTCT GATCTTCTGG CTCATCTTCA GCATCATGGG TGTTAACCTC 
TTTGCTGGGA AATATTATCG ATGCATCAAC ACGACCACGT CAGAATTATT TGAGATCGAT 
GTGGTCAACA ACCGCTCGGA CTGCCTCGCT TTAAACAACT CCGAGCAAGT GCGATGGGTT 
AATGTCAAAG TGAATTTTGA CAATGTGGGC CTTGGGTATC TCTCCCTTCT ACAAGTGGCA 
ACCTTCAAAG GCTGGATGGA TATCATGTAT GCCGCTGTAG ATTCCCGGAA TATTGAGGAT 
CAGCCACAAT ATGAAATAAA TCTTTATATG TATTTGTACT TTGTAATATT CATTATTTTT 
GGAGCTTTCT TCACTCTAAA CCTGTTTATC GGTGTCATTA TCGACAACTT CAATCAGCAA 
AAGAAAAAGT TTGGAGGTAA GGATATCTTT ATGACTGAGG AACAGAAGAA ATACTACAAT 
GCTATGAAAA AGCTGGGATC CAAAAAACCC CAAAAGCCAA TTCCAAGACC CACGGTATTG 
TTCTCTCGGA TCTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scn4a ( XM_018089322.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017944811.2 Xenopus tropicalis sodium channel, voltage gated, type IV alpha subunit (scn4a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa50618
Clone ID Related Accession (Same CDS sequence)	XM_018089322.2 , XM_031894526.1 , XM_031894527.1
Accession Version	XM_018089322.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sodium channel protein type 4 subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018089322.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGCA AGGTGCACCC TCCCGGTCCT GATAGTTTGC GTCCATTTAC CAGAGAGTCC 
TTGGCTGCGA TAGAGAAGCG CATTGAGGAG GACATCGCTC GGAAAGCCAA ACAGATTGAG 
GTGCTCGATG AGAAAGTACC GACACCCAAA AATGGACTGG AGGCAGGAAA GAACTTGCCT 
CTTATATATG GGGATCCCCC ACCCGAACTT ATTGGGATTC CCATAGAGGA ACTAGATTCT 
TTTTACAGCA ACCAAAAGAC ATTTATCGTC TTAAACAAAG GAAAAGCAAT ATTCCGATTC 
ACAGCCACCA AAGCCTTATA TCTACTGGAT CCATTTAATC CAATCAGAAG AGGCGCTATT 
AAAGTCTTAA TCCATTCATT GTTTAGCTTA TTTATTATGT TTACCATCTT AACAAATTGT 
GTTTTTATGA CCATGAGTGA CCCACCACCC TGGTCCAAGA ATGTAGAGTA CACGTTTACT 
GGAATTTACA CCTTTGAGTC CTTAATAAAA ATCCTGGCTC GGGGTTTTTG TATTGACAGT 
TTTACATTCC TCAGGGATCC GTGGAACTGG CTGGATTTCT GTGTCATTGT TATGGCTTAC 
ACCACAGAGT TTGTAGATTT GGGGAATGTG TCTGCCCTTA GGACATTCCG TGTACTCCGT 
GCTCTTAAAA CAATTACAGT TATTCCAGGC CTGAAGACTA TCGTTGGTGC CTTGATCCAA 
TCAGTGAAGA AGCTCGCAGA TGTGATGATC CTCACCCTCT TCTGCCTTGC TGTCTTTGCC 
CTCATTGGCC TTCAGCTCTT TATGGGAAAT CTGAGGCACA AATGTGTCCG ATGGCCACCC 
CCTGATGACT ATTTCATGCT TTATGACAAC AGTACCTTAA ACTCCACACT ATATAATGAT 
ACAATGATCA ACTCCACTCT CAGCAACAGC ACAAATGTGT TCGACAACGA CACCATTGAC 
TGGGGTGCCT ATCTCAACGA TGAAGCAAAT TTTTACTTCC TGGATGGAGC AAATGATGCA 
CTTCTGTGTG GGAACAGCAG TGATGCCGGG AAATGTCCAG AAAGTTACAC ATGTATGAAA 
ACTGGGAGAA ATCCGAATTA TGGGTACACC AGCTACGACT CATTCAACTG GGCTTTCCTT 
TCCCTTTTCC GATTAATGAC ACAGGACTTC TGGGAGAATC TTTTCCAGTT GACACTACGA 
GCAGCTGGTA AAACCTACAT GGTCTTCTTT GTGGTGGTAA TTTTTCTGGG GTCTTTCTAT 
CTGATCAACT TGATCCTTGC TGTGGTGGCC ATGGCATATG CAGAACAGAA TGAAGCCACT 
CTGCAAGAAG AAATAGAAAA GGAGAAAGAG TTCCAGGAGC TTTTGTTGCA GCTAAAGAAA 
CATCAAGAAG AGCAAGCAAG AAGGAAATCC AGCGTTGCCT CAGTTGGACT TCTGAACGCA 
GAAAAGAAAA ATGCTCTTTC AGAGAACACT CTTTCTGGGG ATGGCACAGA AAATATGGAC 
TGCAATGGGC GAACTGTGCC CAAAGTTATA ATTGAACGTG CACCCTCAAT GGAGGATACA 
AGCTCAGCAC AGTCAGACCA ACAGAGGAAT TCTAAAGTTT ATTTGGAGGA GCCAACTCTG 
CAGAAAAGAG CAGAGAGCAC CCACAGTGTC CTATCGGAAG TAGCTCTTGA AGAACTGGAA 
GAGGCTCATC AGAAATGCCC TCCTTGGTGG TACAAGTTTG CTAACACCTT TTTGATTTGG 
GACTGCTGTG ACCTGTGGGT GAAATTTAAG GAATTTGTAA GGTTTGTGGT CATGGATCCC 
TTTGTTGACC TGGGAATCAC CATTTGCATC GTGCTTAACA CCCTTTTTAT GGCCATGGAG 
CACTACCCAA TGACTGAGCA GTTTGAGGGT GTTCTGAATG TAGGAAACTT GGTCTTTACT 
GGCATATTTG CAGCTGAAAT GTTCTTCAAG ATCATAGCCC TGGATCCATA TTACTACTTC 
CAAGTGGGGT GGAACATATT CGACAGCATT ATAGTCACTT TAAGTTTGGT GGAGTTGGGC 
CTTGCAAATG TGGAAGGTCT GTCCGTGCTC AGATCGTTCC GTCTGCTCAG GGTGTTCAAG 
CTAGCCAAAT CATGGCCAAC CCTTAACATG CTTATCAAAA TCATCGGTAA CTCGGTCGGA 
GCACTTGGAA ACCTCACCTT GGTGTTGGCC ATCATTGTCT TCATCTTTGC GGTGGTGGGG 
ATGCAGCTAT TTGGGAAGAG CTACAAGGAA TGTGTGTGCA AGATTGCCTC TGACTGCGAG 
CTACCACGGT GGCACATGCA CGATTTCTTC CACTCTTTTC TCATTGTCTT CCGTATCCTG 
TGCGGGGAGT GGATAGAGAC AATGTGGGAC TGCATGGAAG TGGCAGGGCA AGCAATGTGC 
CTGGTCGTGT TCATGATGGT CATGGTGATT GGTAACCTGG TGGTCCTCAA TCTCTTTTTG 
GCTTTGTTGC TGAGTTCCTT CAGCGCGGAT AACTTGTCGG CTTCGGACGA CGATGGGGAG 
ATGAACAACC TGCAGATCGC CATCGGAAGG ATCACCAGGG GCATTGACTT TGTGAAGAAA 
ACCTTCTTCT CCATCCTACA TTACAAGAGG AAGGCAAAAG CTGAGCAGGA GGAAGAGGAC 
CCTAACAAAG AAAACATTGC TCTGAACCAT GTAGAGACAG AACCAGAACC CAAGATAGAA 
CTAGAACCCA GGACAGAACC AGAACCCAAA AAAGAACCTC CACTGATTGA TGGCGTGACT 
AAAAAACGTT ATTACATGGA TGAGTTTGAC CACGCTAATT TTTTTAACAA CCCACACATG 
AGAATAGAAG TACCTATTGC CTCAGAAGAG TCCGACTTCG ACATTCCAGA CACGGATGAG 
TTTAGCACAC AGTCGGAGGT AGAGGAAGGG ATTTTACACC ACAAGAAGTA CGATGATGGC 
TCGTCAGTGT GTAGCACAGC AGATTATAAA CCTCCTGAAC CTGAAGAGGA AGAGGAGGCC 
TTTGAACACA AAGAAGACGA AGGCCCAGAA GAATGCTTTA CTGCTGACTG TGTACGAAGA 
TGCCCTTGTC TTTATGTCGA CATCACAACA GAAAAAGGGA AAATTTGGTG GAACATTCGG 
AAAACATGTT TCCAGATTGT GGAGCATAAC TGGTTTGAAA CCTTCATCAT CTTCATGATC 
TTACTCAGTA GTGGAGCACT GGCATTTGAG GATATCTACA TTGAACAGAG GCAAGTGATA 
AAGACTATCC TGGAGTACGC TGATAAAGTC TTCACATATA TTTTTGTGTT TGAAATGCTG 
CTAAAGTGGA CAGCGTATGG CTTCAAGATC TATTTCACGA ATGCCTGGTG CTGGCTGGAC 
TTCCTAATTG TTGACGTGTC TTTAATCAGC CTGACAGCCA ACTGGCTGGG CTACTCGGAA 
CTTGGTCCCA TCAAATCCCT TAGAACGCTG AGGGCCCTTA GGCCGTTACG AGCGTTATCA 
AGATTTGAAG GCATGAGGGT GGTTGTAAAC GCCCTTCTGG GAGCCATTCC TTCTATCATG 
AATGTCCTGC TTGTTTGTCT GATCTTCTGG CTCATCTTCA GCATCATGGG TGTTAACCTC 
TTTGCTGGGA AATATTATCG ATGCATCAAC ACGACCACGT CAGAATTATT TGAGATCGAT 
GTGGTCAACA ACCGCTCGGA CTGCCTCGCT TTAAACAACT CCGAGCAAGT GCGATGGGTT 
AATGTCAAAG TGAATTTTGA CAATGTGGGC CTTGGGTATC TCTCCCTTCT ACAAGTGGCA 
ACCTTCAAAG GCTGGATGGA TATCATGTAT GCCGCTGTAG ATTCCCGGAA TATTGAGGAT 
CAGCCACAAT ATGAAATAAA TCTTTATATG TATTTGTACT TTGTAATATT CATTATTTTT 
GGAGCTTTCT TCACTCTAAA CCTGTTTATC GGTGTCATTA TCGACAACTT CAATCAGCAA 
AAGAAAAAGT TTGGAGGTAA GGATATCTTT ATGACTGAGG AACAGAAGAA ATACTACAAT 
GCTATGAAAA AGCTGGGATC CAAAAAACCC CAAAAGCCAA TTCCAAGACC CACGAACAAA 
ATTCAAGGTT TGGTGTATGA CTTTGTCACA AAGCAGCTCT TTGACATTGT GATTATGATT 
TTAATTTGCC TCAACATGGT GACGATGATG ATTGAAACAG ATGACCAGAG TCAGGAAAAA 
ACTGATATTC TTTTCAAGAT CAATCTGGTG TTCATTGTCA TCTTCACAGC CGAATGTGTT 
CTCAAAATGT TTGCGCTGAG GTACTACTTT TTCACAATAG GATGGAACAT ATTTGACTTT 
GTGGTGGTCA TCCTATCTAT TGTTGGTATT GTTCTCTCGG ATCTCATAGC AGAGTACTTT 
GTTTCACCAA CACTTTTCAG AGTCATCAGA CTGGCGAGAA TCGGTAGAGT TCTTCGACTC 
ATAAGAGGAG CAAAGGGGAT TCGCACATTA CTTTTTGCCC TGATGATGTC TCTCCCCGCT 
CTCTTTAATA TCGGGCTTTT GCTTTTCTTA GTTATGTTCA TCTATTCAAT CTTTGGAATG 
TCCAATTTTG CCTATGTAAA GAAAGAATCT GGAATAGATG ACATATTTAA TTTTGAAACC 
TTTGGAAACA GCATAATATG CTTGTTCCAA ATCACCACGT CTGCTGGTTG GGATGGTCTT 
TTAAATCCAA TCCTAAATAG CGGCCCACCA GATTGTGACC CCCATCTGGA GAACTTTGGA 
ACAACAGTTA CTGGTGATTG TGGTAACCCA GCTATAGGTA TTGTGTTCTT CTGCAGCTAC 
ATCATAATAT CATTTTTAAT AGTTGTCAAT ATGTACATTG CCATTATCCT GGAGAACTTT 
AATGTTGCCA CAGAAGAGAG TGGTGAGCCA TTATGTGAGG ATGACTTTGA GATGTTTTAT 
GAAACTTGGG AGAAGTTTGA TCCAGATGCC ACTCAGTTTG TTGAATACAG TCGAATGTCA 
GACTTTGTAG ACACTTTGCA AGATCCTCTA CGGATTCCGA AACCTAACAA AATAAAACTA 
ATTACTTTGG ATCTCCCAAT GGTAACTGGA GATAAGATTC ACTGCTTGGA TATTCTGTTT 
GCACTAACTA AAGAGGTGTT GGGGGACTCA GGAGAAATGG ATGCATTAAA AGCCTCAATG 
GAAGAAAAGT TTATGATGGC AAATCCATCA AAAGTGTCTT ATGAACCAAT CACCACCACC 
CTTAAAAGAA AGCAGGAAGA AGTCTGTGTC ATCAAAATCC AGAGGGCCTT TAGGAGACAT 
CTATTGAGAC GTTCGGTGAA ACACGCTTCC TACATCTTCA GGCAAAGTAA TGATGAAGAG 
TCATCAGTGG AGGAACCTCC TGAAAAAGAA GGGTTGATAG CTGAAAGAAT TAATGCAGCC 
TACGGAAAAT ATGTAGAGGA AGGGCAAAAC GGCGGAACTC ATCGCCCAAG ATCAACAAAC 
AGAACTTCAC ACAGCAGTAT AGATTATGAT GACTCCAAAT CAAAAGCTCT TGCCTTCTTC 
CTGTTGGAAC TGACAGAGGG AACACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017944811.2
CDS	158..5707
Translation	MAGKVHPPGPDSLRPFTRESLAAIEKRIEEDIARKAKQIEVLDEKVPTPKNGLEAGKNLPLIYGDPPPELIGIPIEELDSFYSNQKTFIVLNKGKAIFRFTATKALYLLDPFNPIRRGAIKVLIHSLFSLFIMFTILTNCVFMTMSDPPPWSKNVEYTFTGIYTFESLIKILARGFCIDSFTFLRDPWNWLDFCVIVMAYTTEFVDLGNVSALRTFRVLRALKTITVIPGLKTIVGALIQSVKKLADVMILTLFCLAVFALIGLQLFMGNLRHKCVRWPPPDDYFMLYDNSTLNSTLYNDTMINSTLSNSTNVFDNDTIDWGAYLNDEANFYFLDGANDALLCGNSSDAGKCPESYTCMKTGRNPNYGYTSYDSFNWAFLSLFRLMTQDFWENLFQLTLRAAGKTYMVFFVVVIFLGSFYLINLILAVVAMAYAEQNEATLQEEIEKEKEFQELLLQLKKHQEEQARRKSSVASVGLLNAEKKNALSENTLSGDGTENMDCNGRTVPKVIIERAPSMEDTSSAQSDQQRNSKVYLEEPTLQKRAESTHSVLSEVALEELEEAHQKCPPWWYKFANTFLIWDCCDLWVKFKEFVRFVVMDPFVDLGITICIVLNTLFMAMEHYPMTEQFEGVLNVGNLVFTGIFAAEMFFKIIALDPYYYFQVGWNIFDSIIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKIASDCELPRWHMHDFFHSFLIVFRILCGEWIETMWDCMEVAGQAMCLVVFMMVMVIGNLVVLNLFLALLLSSFSADNLSASDDDGEMNNLQIAIGRITRGIDFVKKTFFSILHYKRKAKAEQEEEDPNKENIALNHVETEPEPKIELEPRTEPEPKKEPPLIDGVTKKRYYMDEFDHANFFNNPHMRIEVPIASEESDFDIPDTDEFSTQSEVEEGILHHKKYDDGSSVCSTADYKPPEPEEEEEAFEHKEDEGPEECFTADCVRRCPCLYVDITTEKGKIWWNIRKTCFQIVEHNWFETFIIFMILLSSGALAFEDIYIEQRQVIKTILEYADKVFTYIFVFEMLLKWTAYGFKIYFTNAWCWLDFLIVDVSLISLTANWLGYSELGPIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKYYRCINTTTSELFEIDVVNNRSDCLALNNSEQVRWVNVKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSRNIEDQPQYEINLYMYLYFVIFIIFGAFFTLNLFIGVIIDNFNQQKKKFGGKDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPTNKIQGLVYDFVTKQLFDIVIMILICLNMVTMMIETDDQSQEKTDILFKINLVFIVIFTAECVLKMFALRYYFFTIGWNIFDFVVVILSIVGIVLSDLIAEYFVSPTLFRVIRLARIGRVLRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMSNFAYVKKESGIDDIFNFETFGNSIICLFQITTSAGWDGLLNPILNSGPPDCDPHLENFGTTVTGDCGNPAIGIVFFCSYIIISFLIVVNMYIAIILENFNVATEESGEPLCEDDFEMFYETWEKFDPDATQFVEYSRMSDFVDTLQDPLRIPKPNKIKLITLDLPMVTGDKIHCLDILFALTKEVLGDSGEMDALKASMEEKFMMANPSKVSYEPITTTLKRKQEEVCVIKIQRAFRRHLLRRSVKHASYIFRQSNDEESSVEEPPEKEGLIAERINAAYGKYVEEGQNGGTHRPRSTNRTSHSSIDYDDSKSKALAFFLLELTEGTQ

Target ORF information:

RefSeq Version	XM_018089322.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sodium channel, voltage gated, type IV alpha subunit (scn4a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018089322.2

ORF Insert Sequence:

ATGGCTGGCA AGGTGCACCC TCCCGGTCCT GATAGTTTGC GTCCATTTAC CAGAGAGTCC 
TTGGCTGCGA TAGAGAAGCG CATTGAGGAG GACATCGCTC GGAAAGCCAA ACAGATTGAG 
GTGCTCGATG AGAAAGTACC GACACCCAAA AATGGACTGG AGGCAGGAAA GAACTTGCCT 
CTTATATATG GGGATCCCCC ACCCGAACTT ATTGGGATTC CCATAGAGGA ACTAGATTCT 
TTTTACAGCA ACCAAAAGAC ATTTATCGTC TTAAACAAAG GAAAAGCAAT ATTCCGATTC 
ACAGCCACCA AAGCCTTATA TCTACTGGAT CCATTTAATC CAATCAGAAG AGGCGCTATT 
AAAGTCTTAA TCCATTCATT GTTTAGCTTA TTTATTATGT TTACCATCTT AACAAATTGT 
GTTTTTATGA CCATGAGTGA CCCACCACCC TGGTCCAAGA ATGTAGAGTA CACGTTTACT 
GGAATTTACA CCTTTGAGTC CTTAATAAAA ATCCTGGCTC GGGGTTTTTG TATTGACAGT 
TTTACATTCC TCAGGGATCC GTGGAACTGG CTGGATTTCT GTGTCATTGT TATGGCTTAC 
ACCACAGAGT TTGTAGATTT GGGGAATGTG TCTGCCCTTA GGACATTCCG TGTACTCCGT 
GCTCTTAAAA CAATTACAGT TATTCCAGGC CTGAAGACTA TCGTTGGTGC CTTGATCCAA 
TCAGTGAAGA AGCTCGCAGA TGTGATGATC CTCACCCTCT TCTGCCTTGC TGTCTTTGCC 
CTCATTGGCC TTCAGCTCTT TATGGGAAAT CTGAGGCACA AATGTGTCCG ATGGCCACCC 
CCTGATGACT ATTTCATGCT TTATGACAAC AGTACCTTAA ACTCCACACT ATATAATGAT 
ACAATGATCA ACTCCACTCT CAGCAACAGC ACAAATGTGT TCGACAACGA CACCATTGAC 
TGGGGTGCCT ATCTCAACGA TGAAGCAAAT TTTTACTTCC TGGATGGAGC AAATGATGCA 
CTTCTGTGTG GGAACAGCAG TGATGCCGGG AAATGTCCAG AAAGTTACAC ATGTATGAAA 
ACTGGGAGAA ATCCGAATTA TGGGTACACC AGCTACGACT CATTCAACTG GGCTTTCCTT 
TCCCTTTTCC GATTAATGAC ACAGGACTTC TGGGAGAATC TTTTCCAGTT GACACTACGA 
GCAGCTGGTA AAACCTACAT GGTCTTCTTT GTGGTGGTAA TTTTTCTGGG GTCTTTCTAT 
CTGATCAACT TGATCCTTGC TGTGGTGGCC ATGGCATATG CAGAACAGAA TGAAGCCACT 
CTGCAAGAAG AAATAGAAAA GGAGAAAGAG TTCCAGGAGC TTTTGTTGCA GCTAAAGAAA 
CATCAAGAAG AGCAAGCAAG AAGGAAATCC AGCGTTGCCT CAGTTGGACT TCTGAACGCA 
GAAAAGAAAA ATGCTCTTTC AGAGAACACT CTTTCTGGGG ATGGCACAGA AAATATGGAC 
TGCAATGGGC GAACTGTGCC CAAAGTTATA ATTGAACGTG CACCCTCAAT GGAGGATACA 
AGCTCAGCAC AGTCAGACCA ACAGAGGAAT TCTAAAGTTT ATTTGGAGGA GCCAACTCTG 
CAGAAAAGAG CAGAGAGCAC CCACAGTGTC CTATCGGAAG TAGCTCTTGA AGAACTGGAA 
GAGGCTCATC AGAAATGCCC TCCTTGGTGG TACAAGTTTG CTAACACCTT TTTGATTTGG 
GACTGCTGTG ACCTGTGGGT GAAATTTAAG GAATTTGTAA GGTTTGTGGT CATGGATCCC 
TTTGTTGACC TGGGAATCAC CATTTGCATC GTGCTTAACA CCCTTTTTAT GGCCATGGAG 
CACTACCCAA TGACTGAGCA GTTTGAGGGT GTTCTGAATG TAGGAAACTT GGTCTTTACT 
GGCATATTTG CAGCTGAAAT GTTCTTCAAG ATCATAGCCC TGGATCCATA TTACTACTTC 
CAAGTGGGGT GGAACATATT CGACAGCATT ATAGTCACTT TAAGTTTGGT GGAGTTGGGC 
CTTGCAAATG TGGAAGGTCT GTCCGTGCTC AGATCGTTCC GTCTGCTCAG GGTGTTCAAG 
CTAGCCAAAT CATGGCCAAC CCTTAACATG CTTATCAAAA TCATCGGTAA CTCGGTCGGA 
GCACTTGGAA ACCTCACCTT GGTGTTGGCC ATCATTGTCT TCATCTTTGC GGTGGTGGGG 
ATGCAGCTAT TTGGGAAGAG CTACAAGGAA TGTGTGTGCA AGATTGCCTC TGACTGCGAG 
CTACCACGGT GGCACATGCA CGATTTCTTC CACTCTTTTC TCATTGTCTT CCGTATCCTG 
TGCGGGGAGT GGATAGAGAC AATGTGGGAC TGCATGGAAG TGGCAGGGCA AGCAATGTGC 
CTGGTCGTGT TCATGATGGT CATGGTGATT GGTAACCTGG TGGTCCTCAA TCTCTTTTTG 
GCTTTGTTGC TGAGTTCCTT CAGCGCGGAT AACTTGTCGG CTTCGGACGA CGATGGGGAG 
ATGAACAACC TGCAGATCGC CATCGGAAGG ATCACCAGGG GCATTGACTT TGTGAAGAAA 
ACCTTCTTCT CCATCCTACA TTACAAGAGG AAGGCAAAAG CTGAGCAGGA GGAAGAGGAC 
CCTAACAAAG AAAACATTGC TCTGAACCAT GTAGAGACAG AACCAGAACC CAAGATAGAA 
CTAGAACCCA GGACAGAACC AGAACCCAAA AAAGAACCTC CACTGATTGA TGGCGTGACT 
AAAAAACGTT ATTACATGGA TGAGTTTGAC CACGCTAATT TTTTTAACAA CCCACACATG 
AGAATAGAAG TACCTATTGC CTCAGAAGAG TCCGACTTCG ACATTCCAGA CACGGATGAG 
TTTAGCACAC AGTCGGAGGT AGAGGAAGGG ATTTTACACC ACAAGAAGTA CGATGATGGC 
TCGTCAGTGT GTAGCACAGC AGATTATAAA CCTCCTGAAC CTGAAGAGGA AGAGGAGGCC 
TTTGAACACA AAGAAGACGA AGGCCCAGAA GAATGCTTTA CTGCTGACTG TGTACGAAGA 
TGCCCTTGTC TTTATGTCGA CATCACAACA GAAAAAGGGA AAATTTGGTG GAACATTCGG 
AAAACATGTT TCCAGATTGT GGAGCATAAC TGGTTTGAAA CCTTCATCAT CTTCATGATC 
TTACTCAGTA GTGGAGCACT GGCATTTGAG GATATCTACA TTGAACAGAG GCAAGTGATA 
AAGACTATCC TGGAGTACGC TGATAAAGTC TTCACATATA TTTTTGTGTT TGAAATGCTG 
CTAAAGTGGA CAGCGTATGG CTTCAAGATC TATTTCACGA ATGCCTGGTG CTGGCTGGAC 
TTCCTAATTG TTGACGTGTC TTTAATCAGC CTGACAGCCA ACTGGCTGGG CTACTCGGAA 
CTTGGTCCCA TCAAATCCCT TAGAACGCTG AGGGCCCTTA GGCCGTTACG AGCGTTATCA 
AGATTTGAAG GCATGAGGGT GGTTGTAAAC GCCCTTCTGG GAGCCATTCC TTCTATCATG 
AATGTCCTGC TTGTTTGTCT GATCTTCTGG CTCATCTTCA GCATCATGGG TGTTAACCTC 
TTTGCTGGGA AATATTATCG ATGCATCAAC ACGACCACGT CAGAATTATT TGAGATCGAT 
GTGGTCAACA ACCGCTCGGA CTGCCTCGCT TTAAACAACT CCGAGCAAGT GCGATGGGTT 
AATGTCAAAG TGAATTTTGA CAATGTGGGC CTTGGGTATC TCTCCCTTCT ACAAGTGGCA 
ACCTTCAAAG GCTGGATGGA TATCATGTAT GCCGCTGTAG ATTCCCGGAA TATTGAGGAT 
CAGCCACAAT ATGAAATAAA TCTTTATATG TATTTGTACT TTGTAATATT CATTATTTTT 
GGAGCTTTCT TCACTCTAAA CCTGTTTATC GGTGTCATTA TCGACAACTT CAATCAGCAA 
AAGAAAAAGT TTGGAGGTAA GGATATCTTT ATGACTGAGG AACAGAAGAA ATACTACAAT 
GCTATGAAAA AGCTGGGATC CAAAAAACCC CAAAAGCCAA TTCCAAGACC CACGAACAAA 
ATTCAAGGTT TGGTGTATGA CTTTGTCACA AAGCAGCTCT TTGACATTGT GATTATGATT 
TTAATTTGCC TCAACATGGT GACGATGATG ATTGAAACAG ATGACCAGAG TCAGGAAAAA 
ACTGATATTC TTTTCAAGAT CAATCTGGTG TTCATTGTCA TCTTCACAGC CGAATGTGTT 
CTCAAAATGT TTGCGCTGAG GTACTACTTT TTCACAATAG GATGGAACAT ATTTGACTTT 
GTGGTGGTCA TCCTATCTAT TGTTGGTATT GTTCTCTCGG ATCTCATAGC AGAGTACTTT 
GTTTCACCAA CACTTTTCAG AGTCATCAGA CTGGCGAGAA TCGGTAGAGT TCTTCGACTC 
ATAAGAGGAG CAAAGGGGAT TCGCACATTA CTTTTTGCCC TGATGATGTC TCTCCCCGCT 
CTCTTTAATA TCGGGCTTTT GCTTTTCTTA GTTATGTTCA TCTATTCAAT CTTTGGAATG 
TCCAATTTTG CCTATGTAAA GAAAGAATCT GGAATAGATG ACATATTTAA TTTTGAAACC 
TTTGGAAACA GCATAATATG CTTGTTCCAA ATCACCACGT CTGCTGGTTG GGATGGTCTT 
TTAAATCCAA TCCTAAATAG CGGCCCACCA GATTGTGACC CCCATCTGGA GAACTTTGGA 
ACAACAGTTA CTGGTGATTG TGGTAACCCA GCTATAGGTA TTGTGTTCTT CTGCAGCTAC 
ATCATAATAT CATTTTTAAT AGTTGTCAAT ATGTACATTG CCATTATCCT GGAGAACTTT 
AATGTTGCCA CAGAAGAGAG TGGTGAGCCA TTATGTGAGG ATGACTTTGA GATGTTTTAT 
GAAACTTGGG AGAAGTTTGA TCCAGATGCC ACTCAGTTTG TTGAATACAG TCGAATGTCA 
GACTTTGTAG ACACTTTGCA AGATCCTCTA CGGATTCCGA AACCTAACAA AATAAAACTA 
ATTACTTTGG ATCTCCCAAT GGTAACTGGA GATAAGATTC ACTGCTTGGA TATTCTGTTT 
GCACTAACTA AAGAGGTGTT GGGGGACTCA GGAGAAATGG ATGCATTAAA AGCCTCAATG 
GAAGAAAAGT TTATGATGGC AAATCCATCA AAAGTGTCTT ATGAACCAAT CACCACCACC 
CTTAAAAGAA AGCAGGAAGA AGTCTGTGTC ATCAAAATCC AGAGGGCCTT TAGGAGACAT 
CTATTGAGAC GTTCGGTGAA ACACGCTTCC TACATCTTCA GGCAAAGTAA TGATGAAGAG 
TCATCAGTGG AGGAACCTCC TGAAAAAGAA GGGTTGATAG CTGAAAGAAT TAATGCAGCC 
TACGGAAAAT ATGTAGAGGA AGGGCAAAAC GGCGGAACTC ATCGCCCAAG ATCAACAAAC 
AGAACTTCAC ACAGCAGTAT AGATTATGAT GACTCCAAAT CAAAAGCTCT TGCCTTCTTC 
CTGTTGGAAC TGACAGAGGG AACACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scn4a ( XM_031894526.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031750386.1 Xenopus tropicalis sodium channel, voltage gated, type IV alpha subunit (scn4a), transcript variant X2, mRNA.

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CloneID	OXa50618
Clone ID Related Accession (Same CDS sequence)	XM_018089322.2 , XM_031894526.1 , XM_031894527.1
Accession Version	XM_031894526.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sodium channel protein type 4 subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGCA AGGTGCACCC TCCCGGTCCT GATAGTTTGC GTCCATTTAC CAGAGAGTCC 
TTGGCTGCGA TAGAGAAGCG CATTGAGGAG GACATCGCTC GGAAAGCCAA ACAGATTGAG 
GTGCTCGATG AGAAAGTACC GACACCCAAA AATGGACTGG AGGCAGGAAA GAACTTGCCT 
CTTATATATG GGGATCCCCC ACCCGAACTT ATTGGGATTC CCATAGAGGA ACTAGATTCT 
TTTTACAGCA ACCAAAAGAC ATTTATCGTC TTAAACAAAG GAAAAGCAAT ATTCCGATTC 
ACAGCCACCA AAGCCTTATA TCTACTGGAT CCATTTAATC CAATCAGAAG AGGCGCTATT 
AAAGTCTTAA TCCATTCATT GTTTAGCTTA TTTATTATGT TTACCATCTT AACAAATTGT 
GTTTTTATGA CCATGAGTGA CCCACCACCC TGGTCCAAGA ATGTAGAGTA CACGTTTACT 
GGAATTTACA CCTTTGAGTC CTTAATAAAA ATCCTGGCTC GGGGTTTTTG TATTGACAGT 
TTTACATTCC TCAGGGATCC GTGGAACTGG CTGGATTTCT GTGTCATTGT TATGGCTTAC 
ACCACAGAGT TTGTAGATTT GGGGAATGTG TCTGCCCTTA GGACATTCCG TGTACTCCGT 
GCTCTTAAAA CAATTACAGT TATTCCAGGC CTGAAGACTA TCGTTGGTGC CTTGATCCAA 
TCAGTGAAGA AGCTCGCAGA TGTGATGATC CTCACCCTCT TCTGCCTTGC TGTCTTTGCC 
CTCATTGGCC TTCAGCTCTT TATGGGAAAT CTGAGGCACA AATGTGTCCG ATGGCCACCC 
CCTGATGACT ATTTCATGCT TTATGACAAC AGTACCTTAA ACTCCACACT ATATAATGAT 
ACAATGATCA ACTCCACTCT CAGCAACAGC ACAAATGTGT TCGACAACGA CACCATTGAC 
TGGGGTGCCT ATCTCAACGA TGAAGCAAAT TTTTACTTCC TGGATGGAGC AAATGATGCA 
CTTCTGTGTG GGAACAGCAG TGATGCCGGG AAATGTCCAG AAAGTTACAC ATGTATGAAA 
ACTGGGAGAA ATCCGAATTA TGGGTACACC AGCTACGACT CATTCAACTG GGCTTTCCTT 
TCCCTTTTCC GATTAATGAC ACAGGACTTC TGGGAGAATC TTTTCCAGTT GACACTACGA 
GCAGCTGGTA AAACCTACAT GGTCTTCTTT GTGGTGGTAA TTTTTCTGGG GTCTTTCTAT 
CTGATCAACT TGATCCTTGC TGTGGTGGCC ATGGCATATG CAGAACAGAA TGAAGCCACT 
CTGCAAGAAG AAATAGAAAA GGAGAAAGAG TTCCAGGAGC TTTTGTTGCA GCTAAAGAAA 
CATCAAGAAG AGCAAGCAAG AAGGAAATCC AGCGTTGCCT CAGTTGGACT TCTGAACGCA 
GAAAAGAAAA ATGCTCTTTC AGAGAACACT CTTTCTGGGG ATGGCACAGA AAATATGGAC 
TGCAATGGGC GAACTGTGCC CAAAGTTATA ATTGAACGTG CACCCTCAAT GGAGGATACA 
AGCTCAGCAC AGTCAGACCA ACAGAGGAAT TCTAAAGTTT ATTTGGAGGA GCCAACTCTG 
CAGAAAAGAG CAGAGAGCAC CCACAGTGTC CTATCGGAAG TAGCTCTTGA AGAACTGGAA 
GAGGCTCATC AGAAATGCCC TCCTTGGTGG TACAAGTTTG CTAACACCTT TTTGATTTGG 
GACTGCTGTG ACCTGTGGGT GAAATTTAAG GAATTTGTAA GGTTTGTGGT CATGGATCCC 
TTTGTTGACC TGGGAATCAC CATTTGCATC GTGCTTAACA CCCTTTTTAT GGCCATGGAG 
CACTACCCAA TGACTGAGCA GTTTGAGGGT GTTCTGAATG TAGGAAACTT GGTCTTTACT 
GGCATATTTG CAGCTGAAAT GTTCTTCAAG ATCATAGCCC TGGATCCATA TTACTACTTC 
CAAGTGGGGT GGAACATATT CGACAGCATT ATAGTCACTT TAAGTTTGGT GGAGTTGGGC 
CTTGCAAATG TGGAAGGTCT GTCCGTGCTC AGATCGTTCC GTCTGCTCAG GGTGTTCAAG 
CTAGCCAAAT CATGGCCAAC CCTTAACATG CTTATCAAAA TCATCGGTAA CTCGGTCGGA 
GCACTTGGAA ACCTCACCTT GGTGTTGGCC ATCATTGTCT TCATCTTTGC GGTGGTGGGG 
ATGCAGCTAT TTGGGAAGAG CTACAAGGAA TGTGTGTGCA AGATTGCCTC TGACTGCGAG 
CTACCACGGT GGCACATGCA CGATTTCTTC CACTCTTTTC TCATTGTCTT CCGTATCCTG 
TGCGGGGAGT GGATAGAGAC AATGTGGGAC TGCATGGAAG TGGCAGGGCA AGCAATGTGC 
CTGGTCGTGT TCATGATGGT CATGGTGATT GGTAACCTGG TGGTCCTCAA TCTCTTTTTG 
GCTTTGTTGC TGAGTTCCTT CAGCGCGGAT AACTTGTCGG CTTCGGACGA CGATGGGGAG 
ATGAACAACC TGCAGATCGC CATCGGAAGG ATCACCAGGG GCATTGACTT TGTGAAGAAA 
ACCTTCTTCT CCATCCTACA TTACAAGAGG AAGGCAAAAG CTGAGCAGGA GGAAGAGGAC 
CCTAACAAAG AAAACATTGC TCTGAACCAT GTAGAGACAG AACCAGAACC CAAGATAGAA 
CTAGAACCCA GGACAGAACC AGAACCCAAA AAAGAACCTC CACTGATTGA TGGCGTGACT 
AAAAAACGTT ATTACATGGA TGAGTTTGAC CACGCTAATT TTTTTAACAA CCCACACATG 
AGAATAGAAG TACCTATTGC CTCAGAAGAG TCCGACTTCG ACATTCCAGA CACGGATGAG 
TTTAGCACAC AGTCGGAGGT AGAGGAAGGG ATTTTACACC ACAAGAAGTA CGATGATGGC 
TCGTCAGTGT GTAGCACAGC AGATTATAAA CCTCCTGAAC CTGAAGAGGA AGAGGAGGCC 
TTTGAACACA AAGAAGACGA AGGCCCAGAA GAATGCTTTA CTGCTGACTG TGTACGAAGA 
TGCCCTTGTC TTTATGTCGA CATCACAACA GAAAAAGGGA AAATTTGGTG GAACATTCGG 
AAAACATGTT TCCAGATTGT GGAGCATAAC TGGTTTGAAA CCTTCATCAT CTTCATGATC 
TTACTCAGTA GTGGAGCACT GGCATTTGAG GATATCTACA TTGAACAGAG GCAAGTGATA 
AAGACTATCC TGGAGTACGC TGATAAAGTC TTCACATATA TTTTTGTGTT TGAAATGCTG 
CTAAAGTGGA CAGCGTATGG CTTCAAGATC TATTTCACGA ATGCCTGGTG CTGGCTGGAC 
TTCCTAATTG TTGACGTGTC TTTAATCAGC CTGACAGCCA ACTGGCTGGG CTACTCGGAA 
CTTGGTCCCA TCAAATCCCT TAGAACGCTG AGGGCCCTTA GGCCGTTACG AGCGTTATCA 
AGATTTGAAG GCATGAGGGT GGTTGTAAAC GCCCTTCTGG GAGCCATTCC TTCTATCATG 
AATGTCCTGC TTGTTTGTCT GATCTTCTGG CTCATCTTCA GCATCATGGG TGTTAACCTC 
TTTGCTGGGA AATATTATCG ATGCATCAAC ACGACCACGT CAGAATTATT TGAGATCGAT 
GTGGTCAACA ACCGCTCGGA CTGCCTCGCT TTAAACAACT CCGAGCAAGT GCGATGGGTT 
AATGTCAAAG TGAATTTTGA CAATGTGGGC CTTGGGTATC TCTCCCTTCT ACAAGTGGCA 
ACCTTCAAAG GCTGGATGGA TATCATGTAT GCCGCTGTAG ATTCCCGGAA TATTGAGGAT 
CAGCCACAAT ATGAAATAAA TCTTTATATG TATTTGTACT TTGTAATATT CATTATTTTT 
GGAGCTTTCT TCACTCTAAA CCTGTTTATC GGTGTCATTA TCGACAACTT CAATCAGCAA 
AAGAAAAAGT TTGGAGGTAA GGATATCTTT ATGACTGAGG AACAGAAGAA ATACTACAAT 
GCTATGAAAA AGCTGGGATC CAAAAAACCC CAAAAGCCAA TTCCAAGACC CACGAACAAA 
ATTCAAGGTT TGGTGTATGA CTTTGTCACA AAGCAGCTCT TTGACATTGT GATTATGATT 
TTAATTTGCC TCAACATGGT GACGATGATG ATTGAAACAG ATGACCAGAG TCAGGAAAAA 
ACTGATATTC TTTTCAAGAT CAATCTGGTG TTCATTGTCA TCTTCACAGC CGAATGTGTT 
CTCAAAATGT TTGCGCTGAG GTACTACTTT TTCACAATAG GATGGAACAT ATTTGACTTT 
GTGGTGGTCA TCCTATCTAT TGTTGGTATT GTTCTCTCGG ATCTCATAGC AGAGTACTTT 
GTTTCACCAA CACTTTTCAG AGTCATCAGA CTGGCGAGAA TCGGTAGAGT TCTTCGACTC 
ATAAGAGGAG CAAAGGGGAT TCGCACATTA CTTTTTGCCC TGATGATGTC TCTCCCCGCT 
CTCTTTAATA TCGGGCTTTT GCTTTTCTTA GTTATGTTCA TCTATTCAAT CTTTGGAATG 
TCCAATTTTG CCTATGTAAA GAAAGAATCT GGAATAGATG ACATATTTAA TTTTGAAACC 
TTTGGAAACA GCATAATATG CTTGTTCCAA ATCACCACGT CTGCTGGTTG GGATGGTCTT 
TTAAATCCAA TCCTAAATAG CGGCCCACCA GATTGTGACC CCCATCTGGA GAACTTTGGA 
ACAACAGTTA CTGGTGATTG TGGTAACCCA GCTATAGGTA TTGTGTTCTT CTGCAGCTAC 
ATCATAATAT CATTTTTAAT AGTTGTCAAT ATGTACATTG CCATTATCCT GGAGAACTTT 
AATGTTGCCA CAGAAGAGAG TGGTGAGCCA TTATGTGAGG ATGACTTTGA GATGTTTTAT 
GAAACTTGGG AGAAGTTTGA TCCAGATGCC ACTCAGTTTG TTGAATACAG TCGAATGTCA 
GACTTTGTAG ACACTTTGCA AGATCCTCTA CGGATTCCGA AACCTAACAA AATAAAACTA 
ATTACTTTGG ATCTCCCAAT GGTAACTGGA GATAAGATTC ACTGCTTGGA TATTCTGTTT 
GCACTAACTA AAGAGGTGTT GGGGGACTCA GGAGAAATGG ATGCATTAAA AGCCTCAATG 
GAAGAAAAGT TTATGATGGC AAATCCATCA AAAGTGTCTT ATGAACCAAT CACCACCACC 
CTTAAAAGAA AGCAGGAAGA AGTCTGTGTC ATCAAAATCC AGAGGGCCTT TAGGAGACAT 
CTATTGAGAC GTTCGGTGAA ACACGCTTCC TACATCTTCA GGCAAAGTAA TGATGAAGAG 
TCATCAGTGG AGGAACCTCC TGAAAAAGAA GGGTTGATAG CTGAAAGAAT TAATGCAGCC 
TACGGAAAAT ATGTAGAGGA AGGGCAAAAC GGCGGAACTC ATCGCCCAAG ATCAACAAAC 
AGAACTTCAC ACAGCAGTAT AGATTATGAT GACTCCAAAT CAAAAGCTCT TGCCTTCTTC 
CTGTTGGAAC TGACAGAGGG AACACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031750386.1
CDS	323..5872
Translation	MAGKVHPPGPDSLRPFTRESLAAIEKRIEEDIARKAKQIEVLDEKVPTPKNGLEAGKNLPLIYGDPPPELIGIPIEELDSFYSNQKTFIVLNKGKAIFRFTATKALYLLDPFNPIRRGAIKVLIHSLFSLFIMFTILTNCVFMTMSDPPPWSKNVEYTFTGIYTFESLIKILARGFCIDSFTFLRDPWNWLDFCVIVMAYTTEFVDLGNVSALRTFRVLRALKTITVIPGLKTIVGALIQSVKKLADVMILTLFCLAVFALIGLQLFMGNLRHKCVRWPPPDDYFMLYDNSTLNSTLYNDTMINSTLSNSTNVFDNDTIDWGAYLNDEANFYFLDGANDALLCGNSSDAGKCPESYTCMKTGRNPNYGYTSYDSFNWAFLSLFRLMTQDFWENLFQLTLRAAGKTYMVFFVVVIFLGSFYLINLILAVVAMAYAEQNEATLQEEIEKEKEFQELLLQLKKHQEEQARRKSSVASVGLLNAEKKNALSENTLSGDGTENMDCNGRTVPKVIIERAPSMEDTSSAQSDQQRNSKVYLEEPTLQKRAESTHSVLSEVALEELEEAHQKCPPWWYKFANTFLIWDCCDLWVKFKEFVRFVVMDPFVDLGITICIVLNTLFMAMEHYPMTEQFEGVLNVGNLVFTGIFAAEMFFKIIALDPYYYFQVGWNIFDSIIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKIASDCELPRWHMHDFFHSFLIVFRILCGEWIETMWDCMEVAGQAMCLVVFMMVMVIGNLVVLNLFLALLLSSFSADNLSASDDDGEMNNLQIAIGRITRGIDFVKKTFFSILHYKRKAKAEQEEEDPNKENIALNHVETEPEPKIELEPRTEPEPKKEPPLIDGVTKKRYYMDEFDHANFFNNPHMRIEVPIASEESDFDIPDTDEFSTQSEVEEGILHHKKYDDGSSVCSTADYKPPEPEEEEEAFEHKEDEGPEECFTADCVRRCPCLYVDITTEKGKIWWNIRKTCFQIVEHNWFETFIIFMILLSSGALAFEDIYIEQRQVIKTILEYADKVFTYIFVFEMLLKWTAYGFKIYFTNAWCWLDFLIVDVSLISLTANWLGYSELGPIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKYYRCINTTTSELFEIDVVNNRSDCLALNNSEQVRWVNVKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSRNIEDQPQYEINLYMYLYFVIFIIFGAFFTLNLFIGVIIDNFNQQKKKFGGKDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPTNKIQGLVYDFVTKQLFDIVIMILICLNMVTMMIETDDQSQEKTDILFKINLVFIVIFTAECVLKMFALRYYFFTIGWNIFDFVVVILSIVGIVLSDLIAEYFVSPTLFRVIRLARIGRVLRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMSNFAYVKKESGIDDIFNFETFGNSIICLFQITTSAGWDGLLNPILNSGPPDCDPHLENFGTTVTGDCGNPAIGIVFFCSYIIISFLIVVNMYIAIILENFNVATEESGEPLCEDDFEMFYETWEKFDPDATQFVEYSRMSDFVDTLQDPLRIPKPNKIKLITLDLPMVTGDKIHCLDILFALTKEVLGDSGEMDALKASMEEKFMMANPSKVSYEPITTTLKRKQEEVCVIKIQRAFRRHLLRRSVKHASYIFRQSNDEESSVEEPPEKEGLIAERINAAYGKYVEEGQNGGTHRPRSTNRTSHSSIDYDDSKSKALAFFLLELTEGTQ

Target ORF information:

RefSeq Version	XM_031894526.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sodium channel, voltage gated, type IV alpha subunit (scn4a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031894526.1

ORF Insert Sequence:

ATGGCTGGCA AGGTGCACCC TCCCGGTCCT GATAGTTTGC GTCCATTTAC CAGAGAGTCC 
TTGGCTGCGA TAGAGAAGCG CATTGAGGAG GACATCGCTC GGAAAGCCAA ACAGATTGAG 
GTGCTCGATG AGAAAGTACC GACACCCAAA AATGGACTGG AGGCAGGAAA GAACTTGCCT 
CTTATATATG GGGATCCCCC ACCCGAACTT ATTGGGATTC CCATAGAGGA ACTAGATTCT 
TTTTACAGCA ACCAAAAGAC ATTTATCGTC TTAAACAAAG GAAAAGCAAT ATTCCGATTC 
ACAGCCACCA AAGCCTTATA TCTACTGGAT CCATTTAATC CAATCAGAAG AGGCGCTATT 
AAAGTCTTAA TCCATTCATT GTTTAGCTTA TTTATTATGT TTACCATCTT AACAAATTGT 
GTTTTTATGA CCATGAGTGA CCCACCACCC TGGTCCAAGA ATGTAGAGTA CACGTTTACT 
GGAATTTACA CCTTTGAGTC CTTAATAAAA ATCCTGGCTC GGGGTTTTTG TATTGACAGT 
TTTACATTCC TCAGGGATCC GTGGAACTGG CTGGATTTCT GTGTCATTGT TATGGCTTAC 
ACCACAGAGT TTGTAGATTT GGGGAATGTG TCTGCCCTTA GGACATTCCG TGTACTCCGT 
GCTCTTAAAA CAATTACAGT TATTCCAGGC CTGAAGACTA TCGTTGGTGC CTTGATCCAA 
TCAGTGAAGA AGCTCGCAGA TGTGATGATC CTCACCCTCT TCTGCCTTGC TGTCTTTGCC 
CTCATTGGCC TTCAGCTCTT TATGGGAAAT CTGAGGCACA AATGTGTCCG ATGGCCACCC 
CCTGATGACT ATTTCATGCT TTATGACAAC AGTACCTTAA ACTCCACACT ATATAATGAT 
ACAATGATCA ACTCCACTCT CAGCAACAGC ACAAATGTGT TCGACAACGA CACCATTGAC 
TGGGGTGCCT ATCTCAACGA TGAAGCAAAT TTTTACTTCC TGGATGGAGC AAATGATGCA 
CTTCTGTGTG GGAACAGCAG TGATGCCGGG AAATGTCCAG AAAGTTACAC ATGTATGAAA 
ACTGGGAGAA ATCCGAATTA TGGGTACACC AGCTACGACT CATTCAACTG GGCTTTCCTT 
TCCCTTTTCC GATTAATGAC ACAGGACTTC TGGGAGAATC TTTTCCAGTT GACACTACGA 
GCAGCTGGTA AAACCTACAT GGTCTTCTTT GTGGTGGTAA TTTTTCTGGG GTCTTTCTAT 
CTGATCAACT TGATCCTTGC TGTGGTGGCC ATGGCATATG CAGAACAGAA TGAAGCCACT 
CTGCAAGAAG AAATAGAAAA GGAGAAAGAG TTCCAGGAGC TTTTGTTGCA GCTAAAGAAA 
CATCAAGAAG AGCAAGCAAG AAGGAAATCC AGCGTTGCCT CAGTTGGACT TCTGAACGCA 
GAAAAGAAAA ATGCTCTTTC AGAGAACACT CTTTCTGGGG ATGGCACAGA AAATATGGAC 
TGCAATGGGC GAACTGTGCC CAAAGTTATA ATTGAACGTG CACCCTCAAT GGAGGATACA 
AGCTCAGCAC AGTCAGACCA ACAGAGGAAT TCTAAAGTTT ATTTGGAGGA GCCAACTCTG 
CAGAAAAGAG CAGAGAGCAC CCACAGTGTC CTATCGGAAG TAGCTCTTGA AGAACTGGAA 
GAGGCTCATC AGAAATGCCC TCCTTGGTGG TACAAGTTTG CTAACACCTT TTTGATTTGG 
GACTGCTGTG ACCTGTGGGT GAAATTTAAG GAATTTGTAA GGTTTGTGGT CATGGATCCC 
TTTGTTGACC TGGGAATCAC CATTTGCATC GTGCTTAACA CCCTTTTTAT GGCCATGGAG 
CACTACCCAA TGACTGAGCA GTTTGAGGGT GTTCTGAATG TAGGAAACTT GGTCTTTACT 
GGCATATTTG CAGCTGAAAT GTTCTTCAAG ATCATAGCCC TGGATCCATA TTACTACTTC 
CAAGTGGGGT GGAACATATT CGACAGCATT ATAGTCACTT TAAGTTTGGT GGAGTTGGGC 
CTTGCAAATG TGGAAGGTCT GTCCGTGCTC AGATCGTTCC GTCTGCTCAG GGTGTTCAAG 
CTAGCCAAAT CATGGCCAAC CCTTAACATG CTTATCAAAA TCATCGGTAA CTCGGTCGGA 
GCACTTGGAA ACCTCACCTT GGTGTTGGCC ATCATTGTCT TCATCTTTGC GGTGGTGGGG 
ATGCAGCTAT TTGGGAAGAG CTACAAGGAA TGTGTGTGCA AGATTGCCTC TGACTGCGAG 
CTACCACGGT GGCACATGCA CGATTTCTTC CACTCTTTTC TCATTGTCTT CCGTATCCTG 
TGCGGGGAGT GGATAGAGAC AATGTGGGAC TGCATGGAAG TGGCAGGGCA AGCAATGTGC 
CTGGTCGTGT TCATGATGGT CATGGTGATT GGTAACCTGG TGGTCCTCAA TCTCTTTTTG 
GCTTTGTTGC TGAGTTCCTT CAGCGCGGAT AACTTGTCGG CTTCGGACGA CGATGGGGAG 
ATGAACAACC TGCAGATCGC CATCGGAAGG ATCACCAGGG GCATTGACTT TGTGAAGAAA 
ACCTTCTTCT CCATCCTACA TTACAAGAGG AAGGCAAAAG CTGAGCAGGA GGAAGAGGAC 
CCTAACAAAG AAAACATTGC TCTGAACCAT GTAGAGACAG AACCAGAACC CAAGATAGAA 
CTAGAACCCA GGACAGAACC AGAACCCAAA AAAGAACCTC CACTGATTGA TGGCGTGACT 
AAAAAACGTT ATTACATGGA TGAGTTTGAC CACGCTAATT TTTTTAACAA CCCACACATG 
AGAATAGAAG TACCTATTGC CTCAGAAGAG TCCGACTTCG ACATTCCAGA CACGGATGAG 
TTTAGCACAC AGTCGGAGGT AGAGGAAGGG ATTTTACACC ACAAGAAGTA CGATGATGGC 
TCGTCAGTGT GTAGCACAGC AGATTATAAA CCTCCTGAAC CTGAAGAGGA AGAGGAGGCC 
TTTGAACACA AAGAAGACGA AGGCCCAGAA GAATGCTTTA CTGCTGACTG TGTACGAAGA 
TGCCCTTGTC TTTATGTCGA CATCACAACA GAAAAAGGGA AAATTTGGTG GAACATTCGG 
AAAACATGTT TCCAGATTGT GGAGCATAAC TGGTTTGAAA CCTTCATCAT CTTCATGATC 
TTACTCAGTA GTGGAGCACT GGCATTTGAG GATATCTACA TTGAACAGAG GCAAGTGATA 
AAGACTATCC TGGAGTACGC TGATAAAGTC TTCACATATA TTTTTGTGTT TGAAATGCTG 
CTAAAGTGGA CAGCGTATGG CTTCAAGATC TATTTCACGA ATGCCTGGTG CTGGCTGGAC 
TTCCTAATTG TTGACGTGTC TTTAATCAGC CTGACAGCCA ACTGGCTGGG CTACTCGGAA 
CTTGGTCCCA TCAAATCCCT TAGAACGCTG AGGGCCCTTA GGCCGTTACG AGCGTTATCA 
AGATTTGAAG GCATGAGGGT GGTTGTAAAC GCCCTTCTGG GAGCCATTCC TTCTATCATG 
AATGTCCTGC TTGTTTGTCT GATCTTCTGG CTCATCTTCA GCATCATGGG TGTTAACCTC 
TTTGCTGGGA AATATTATCG ATGCATCAAC ACGACCACGT CAGAATTATT TGAGATCGAT 
GTGGTCAACA ACCGCTCGGA CTGCCTCGCT TTAAACAACT CCGAGCAAGT GCGATGGGTT 
AATGTCAAAG TGAATTTTGA CAATGTGGGC CTTGGGTATC TCTCCCTTCT ACAAGTGGCA 
ACCTTCAAAG GCTGGATGGA TATCATGTAT GCCGCTGTAG ATTCCCGGAA TATTGAGGAT 
CAGCCACAAT ATGAAATAAA TCTTTATATG TATTTGTACT TTGTAATATT CATTATTTTT 
GGAGCTTTCT TCACTCTAAA CCTGTTTATC GGTGTCATTA TCGACAACTT CAATCAGCAA 
AAGAAAAAGT TTGGAGGTAA GGATATCTTT ATGACTGAGG AACAGAAGAA ATACTACAAT 
GCTATGAAAA AGCTGGGATC CAAAAAACCC CAAAAGCCAA TTCCAAGACC CACGAACAAA 
ATTCAAGGTT TGGTGTATGA CTTTGTCACA AAGCAGCTCT TTGACATTGT GATTATGATT 
TTAATTTGCC TCAACATGGT GACGATGATG ATTGAAACAG ATGACCAGAG TCAGGAAAAA 
ACTGATATTC TTTTCAAGAT CAATCTGGTG TTCATTGTCA TCTTCACAGC CGAATGTGTT 
CTCAAAATGT TTGCGCTGAG GTACTACTTT TTCACAATAG GATGGAACAT ATTTGACTTT 
GTGGTGGTCA TCCTATCTAT TGTTGGTATT GTTCTCTCGG ATCTCATAGC AGAGTACTTT 
GTTTCACCAA CACTTTTCAG AGTCATCAGA CTGGCGAGAA TCGGTAGAGT TCTTCGACTC 
ATAAGAGGAG CAAAGGGGAT TCGCACATTA CTTTTTGCCC TGATGATGTC TCTCCCCGCT 
CTCTTTAATA TCGGGCTTTT GCTTTTCTTA GTTATGTTCA TCTATTCAAT CTTTGGAATG 
TCCAATTTTG CCTATGTAAA GAAAGAATCT GGAATAGATG ACATATTTAA TTTTGAAACC 
TTTGGAAACA GCATAATATG CTTGTTCCAA ATCACCACGT CTGCTGGTTG GGATGGTCTT 
TTAAATCCAA TCCTAAATAG CGGCCCACCA GATTGTGACC CCCATCTGGA GAACTTTGGA 
ACAACAGTTA CTGGTGATTG TGGTAACCCA GCTATAGGTA TTGTGTTCTT CTGCAGCTAC 
ATCATAATAT CATTTTTAAT AGTTGTCAAT ATGTACATTG CCATTATCCT GGAGAACTTT 
AATGTTGCCA CAGAAGAGAG TGGTGAGCCA TTATGTGAGG ATGACTTTGA GATGTTTTAT 
GAAACTTGGG AGAAGTTTGA TCCAGATGCC ACTCAGTTTG TTGAATACAG TCGAATGTCA 
GACTTTGTAG ACACTTTGCA AGATCCTCTA CGGATTCCGA AACCTAACAA AATAAAACTA 
ATTACTTTGG ATCTCCCAAT GGTAACTGGA GATAAGATTC ACTGCTTGGA TATTCTGTTT 
GCACTAACTA AAGAGGTGTT GGGGGACTCA GGAGAAATGG ATGCATTAAA AGCCTCAATG 
GAAGAAAAGT TTATGATGGC AAATCCATCA AAAGTGTCTT ATGAACCAAT CACCACCACC 
CTTAAAAGAA AGCAGGAAGA AGTCTGTGTC ATCAAAATCC AGAGGGCCTT TAGGAGACAT 
CTATTGAGAC GTTCGGTGAA ACACGCTTCC TACATCTTCA GGCAAAGTAA TGATGAAGAG 
TCATCAGTGG AGGAACCTCC TGAAAAAGAA GGGTTGATAG CTGAAAGAAT TAATGCAGCC 
TACGGAAAAT ATGTAGAGGA AGGGCAAAAC GGCGGAACTC ATCGCCCAAG ATCAACAAAC 
AGAACTTCAC ACAGCAGTAT AGATTATGAT GACTCCAAAT CAAAAGCTCT TGCCTTCTTC 
CTGTTGGAAC TGACAGAGGG AACACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scn4a ( XM_031894527.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031750387.1 Xenopus tropicalis sodium channel, voltage gated, type IV alpha subunit (scn4a), transcript variant X3, mRNA.

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CloneID	OXa50618
Clone ID Related Accession (Same CDS sequence)	XM_018089322.2 , XM_031894526.1 , XM_031894527.1
Accession Version	XM_031894527.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sodium channel protein type 4 subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGCA AGGTGCACCC TCCCGGTCCT GATAGTTTGC GTCCATTTAC CAGAGAGTCC 
TTGGCTGCGA TAGAGAAGCG CATTGAGGAG GACATCGCTC GGAAAGCCAA ACAGATTGAG 
GTGCTCGATG AGAAAGTACC GACACCCAAA AATGGACTGG AGGCAGGAAA GAACTTGCCT 
CTTATATATG GGGATCCCCC ACCCGAACTT ATTGGGATTC CCATAGAGGA ACTAGATTCT 
TTTTACAGCA ACCAAAAGAC ATTTATCGTC TTAAACAAAG GAAAAGCAAT ATTCCGATTC 
ACAGCCACCA AAGCCTTATA TCTACTGGAT CCATTTAATC CAATCAGAAG AGGCGCTATT 
AAAGTCTTAA TCCATTCATT GTTTAGCTTA TTTATTATGT TTACCATCTT AACAAATTGT 
GTTTTTATGA CCATGAGTGA CCCACCACCC TGGTCCAAGA ATGTAGAGTA CACGTTTACT 
GGAATTTACA CCTTTGAGTC CTTAATAAAA ATCCTGGCTC GGGGTTTTTG TATTGACAGT 
TTTACATTCC TCAGGGATCC GTGGAACTGG CTGGATTTCT GTGTCATTGT TATGGCTTAC 
ACCACAGAGT TTGTAGATTT GGGGAATGTG TCTGCCCTTA GGACATTCCG TGTACTCCGT 
GCTCTTAAAA CAATTACAGT TATTCCAGGC CTGAAGACTA TCGTTGGTGC CTTGATCCAA 
TCAGTGAAGA AGCTCGCAGA TGTGATGATC CTCACCCTCT TCTGCCTTGC TGTCTTTGCC 
CTCATTGGCC TTCAGCTCTT TATGGGAAAT CTGAGGCACA AATGTGTCCG ATGGCCACCC 
CCTGATGACT ATTTCATGCT TTATGACAAC AGTACCTTAA ACTCCACACT ATATAATGAT 
ACAATGATCA ACTCCACTCT CAGCAACAGC ACAAATGTGT TCGACAACGA CACCATTGAC 
TGGGGTGCCT ATCTCAACGA TGAAGCAAAT TTTTACTTCC TGGATGGAGC AAATGATGCA 
CTTCTGTGTG GGAACAGCAG TGATGCCGGG AAATGTCCAG AAAGTTACAC ATGTATGAAA 
ACTGGGAGAA ATCCGAATTA TGGGTACACC AGCTACGACT CATTCAACTG GGCTTTCCTT 
TCCCTTTTCC GATTAATGAC ACAGGACTTC TGGGAGAATC TTTTCCAGTT GACACTACGA 
GCAGCTGGTA AAACCTACAT GGTCTTCTTT GTGGTGGTAA TTTTTCTGGG GTCTTTCTAT 
CTGATCAACT TGATCCTTGC TGTGGTGGCC ATGGCATATG CAGAACAGAA TGAAGCCACT 
CTGCAAGAAG AAATAGAAAA GGAGAAAGAG TTCCAGGAGC TTTTGTTGCA GCTAAAGAAA 
CATCAAGAAG AGCAAGCAAG AAGGAAATCC AGCGTTGCCT CAGTTGGACT TCTGAACGCA 
GAAAAGAAAA ATGCTCTTTC AGAGAACACT CTTTCTGGGG ATGGCACAGA AAATATGGAC 
TGCAATGGGC GAACTGTGCC CAAAGTTATA ATTGAACGTG CACCCTCAAT GGAGGATACA 
AGCTCAGCAC AGTCAGACCA ACAGAGGAAT TCTAAAGTTT ATTTGGAGGA GCCAACTCTG 
CAGAAAAGAG CAGAGAGCAC CCACAGTGTC CTATCGGAAG TAGCTCTTGA AGAACTGGAA 
GAGGCTCATC AGAAATGCCC TCCTTGGTGG TACAAGTTTG CTAACACCTT TTTGATTTGG 
GACTGCTGTG ACCTGTGGGT GAAATTTAAG GAATTTGTAA GGTTTGTGGT CATGGATCCC 
TTTGTTGACC TGGGAATCAC CATTTGCATC GTGCTTAACA CCCTTTTTAT GGCCATGGAG 
CACTACCCAA TGACTGAGCA GTTTGAGGGT GTTCTGAATG TAGGAAACTT GGTCTTTACT 
GGCATATTTG CAGCTGAAAT GTTCTTCAAG ATCATAGCCC TGGATCCATA TTACTACTTC 
CAAGTGGGGT GGAACATATT CGACAGCATT ATAGTCACTT TAAGTTTGGT GGAGTTGGGC 
CTTGCAAATG TGGAAGGTCT GTCCGTGCTC AGATCGTTCC GTCTGCTCAG GGTGTTCAAG 
CTAGCCAAAT CATGGCCAAC CCTTAACATG CTTATCAAAA TCATCGGTAA CTCGGTCGGA 
GCACTTGGAA ACCTCACCTT GGTGTTGGCC ATCATTGTCT TCATCTTTGC GGTGGTGGGG 
ATGCAGCTAT TTGGGAAGAG CTACAAGGAA TGTGTGTGCA AGATTGCCTC TGACTGCGAG 
CTACCACGGT GGCACATGCA CGATTTCTTC CACTCTTTTC TCATTGTCTT CCGTATCCTG 
TGCGGGGAGT GGATAGAGAC AATGTGGGAC TGCATGGAAG TGGCAGGGCA AGCAATGTGC 
CTGGTCGTGT TCATGATGGT CATGGTGATT GGTAACCTGG TGGTCCTCAA TCTCTTTTTG 
GCTTTGTTGC TGAGTTCCTT CAGCGCGGAT AACTTGTCGG CTTCGGACGA CGATGGGGAG 
ATGAACAACC TGCAGATCGC CATCGGAAGG ATCACCAGGG GCATTGACTT TGTGAAGAAA 
ACCTTCTTCT CCATCCTACA TTACAAGAGG AAGGCAAAAG CTGAGCAGGA GGAAGAGGAC 
CCTAACAAAG AAAACATTGC TCTGAACCAT GTAGAGACAG AACCAGAACC CAAGATAGAA 
CTAGAACCCA GGACAGAACC AGAACCCAAA AAAGAACCTC CACTGATTGA TGGCGTGACT 
AAAAAACGTT ATTACATGGA TGAGTTTGAC CACGCTAATT TTTTTAACAA CCCACACATG 
AGAATAGAAG TACCTATTGC CTCAGAAGAG TCCGACTTCG ACATTCCAGA CACGGATGAG 
TTTAGCACAC AGTCGGAGGT AGAGGAAGGG ATTTTACACC ACAAGAAGTA CGATGATGGC 
TCGTCAGTGT GTAGCACAGC AGATTATAAA CCTCCTGAAC CTGAAGAGGA AGAGGAGGCC 
TTTGAACACA AAGAAGACGA AGGCCCAGAA GAATGCTTTA CTGCTGACTG TGTACGAAGA 
TGCCCTTGTC TTTATGTCGA CATCACAACA GAAAAAGGGA AAATTTGGTG GAACATTCGG 
AAAACATGTT TCCAGATTGT GGAGCATAAC TGGTTTGAAA CCTTCATCAT CTTCATGATC 
TTACTCAGTA GTGGAGCACT GGCATTTGAG GATATCTACA TTGAACAGAG GCAAGTGATA 
AAGACTATCC TGGAGTACGC TGATAAAGTC TTCACATATA TTTTTGTGTT TGAAATGCTG 
CTAAAGTGGA CAGCGTATGG CTTCAAGATC TATTTCACGA ATGCCTGGTG CTGGCTGGAC 
TTCCTAATTG TTGACGTGTC TTTAATCAGC CTGACAGCCA ACTGGCTGGG CTACTCGGAA 
CTTGGTCCCA TCAAATCCCT TAGAACGCTG AGGGCCCTTA GGCCGTTACG AGCGTTATCA 
AGATTTGAAG GCATGAGGGT GGTTGTAAAC GCCCTTCTGG GAGCCATTCC TTCTATCATG 
AATGTCCTGC TTGTTTGTCT GATCTTCTGG CTCATCTTCA GCATCATGGG TGTTAACCTC 
TTTGCTGGGA AATATTATCG ATGCATCAAC ACGACCACGT CAGAATTATT TGAGATCGAT 
GTGGTCAACA ACCGCTCGGA CTGCCTCGCT TTAAACAACT CCGAGCAAGT GCGATGGGTT 
AATGTCAAAG TGAATTTTGA CAATGTGGGC CTTGGGTATC TCTCCCTTCT ACAAGTGGCA 
ACCTTCAAAG GCTGGATGGA TATCATGTAT GCCGCTGTAG ATTCCCGGAA TATTGAGGAT 
CAGCCACAAT ATGAAATAAA TCTTTATATG TATTTGTACT TTGTAATATT CATTATTTTT 
GGAGCTTTCT TCACTCTAAA CCTGTTTATC GGTGTCATTA TCGACAACTT CAATCAGCAA 
AAGAAAAAGT TTGGAGGTAA GGATATCTTT ATGACTGAGG AACAGAAGAA ATACTACAAT 
GCTATGAAAA AGCTGGGATC CAAAAAACCC CAAAAGCCAA TTCCAAGACC CACGAACAAA 
ATTCAAGGTT TGGTGTATGA CTTTGTCACA AAGCAGCTCT TTGACATTGT GATTATGATT 
TTAATTTGCC TCAACATGGT GACGATGATG ATTGAAACAG ATGACCAGAG TCAGGAAAAA 
ACTGATATTC TTTTCAAGAT CAATCTGGTG TTCATTGTCA TCTTCACAGC CGAATGTGTT 
CTCAAAATGT TTGCGCTGAG GTACTACTTT TTCACAATAG GATGGAACAT ATTTGACTTT 
GTGGTGGTCA TCCTATCTAT TGTTGGTATT GTTCTCTCGG ATCTCATAGC AGAGTACTTT 
GTTTCACCAA CACTTTTCAG AGTCATCAGA CTGGCGAGAA TCGGTAGAGT TCTTCGACTC 
ATAAGAGGAG CAAAGGGGAT TCGCACATTA CTTTTTGCCC TGATGATGTC TCTCCCCGCT 
CTCTTTAATA TCGGGCTTTT GCTTTTCTTA GTTATGTTCA TCTATTCAAT CTTTGGAATG 
TCCAATTTTG CCTATGTAAA GAAAGAATCT GGAATAGATG ACATATTTAA TTTTGAAACC 
TTTGGAAACA GCATAATATG CTTGTTCCAA ATCACCACGT CTGCTGGTTG GGATGGTCTT 
TTAAATCCAA TCCTAAATAG CGGCCCACCA GATTGTGACC CCCATCTGGA GAACTTTGGA 
ACAACAGTTA CTGGTGATTG TGGTAACCCA GCTATAGGTA TTGTGTTCTT CTGCAGCTAC 
ATCATAATAT CATTTTTAAT AGTTGTCAAT ATGTACATTG CCATTATCCT GGAGAACTTT 
AATGTTGCCA CAGAAGAGAG TGGTGAGCCA TTATGTGAGG ATGACTTTGA GATGTTTTAT 
GAAACTTGGG AGAAGTTTGA TCCAGATGCC ACTCAGTTTG TTGAATACAG TCGAATGTCA 
GACTTTGTAG ACACTTTGCA AGATCCTCTA CGGATTCCGA AACCTAACAA AATAAAACTA 
ATTACTTTGG ATCTCCCAAT GGTAACTGGA GATAAGATTC ACTGCTTGGA TATTCTGTTT 
GCACTAACTA AAGAGGTGTT GGGGGACTCA GGAGAAATGG ATGCATTAAA AGCCTCAATG 
GAAGAAAAGT TTATGATGGC AAATCCATCA AAAGTGTCTT ATGAACCAAT CACCACCACC 
CTTAAAAGAA AGCAGGAAGA AGTCTGTGTC ATCAAAATCC AGAGGGCCTT TAGGAGACAT 
CTATTGAGAC GTTCGGTGAA ACACGCTTCC TACATCTTCA GGCAAAGTAA TGATGAAGAG 
TCATCAGTGG AGGAACCTCC TGAAAAAGAA GGGTTGATAG CTGAAAGAAT TAATGCAGCC 
TACGGAAAAT ATGTAGAGGA AGGGCAAAAC GGCGGAACTC ATCGCCCAAG ATCAACAAAC 
AGAACTTCAC ACAGCAGTAT AGATTATGAT GACTCCAAAT CAAAAGCTCT TGCCTTCTTC 
CTGTTGGAAC TGACAGAGGG AACACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031750387.1
CDS	249..5798
Translation	MAGKVHPPGPDSLRPFTRESLAAIEKRIEEDIARKAKQIEVLDEKVPTPKNGLEAGKNLPLIYGDPPPELIGIPIEELDSFYSNQKTFIVLNKGKAIFRFTATKALYLLDPFNPIRRGAIKVLIHSLFSLFIMFTILTNCVFMTMSDPPPWSKNVEYTFTGIYTFESLIKILARGFCIDSFTFLRDPWNWLDFCVIVMAYTTEFVDLGNVSALRTFRVLRALKTITVIPGLKTIVGALIQSVKKLADVMILTLFCLAVFALIGLQLFMGNLRHKCVRWPPPDDYFMLYDNSTLNSTLYNDTMINSTLSNSTNVFDNDTIDWGAYLNDEANFYFLDGANDALLCGNSSDAGKCPESYTCMKTGRNPNYGYTSYDSFNWAFLSLFRLMTQDFWENLFQLTLRAAGKTYMVFFVVVIFLGSFYLINLILAVVAMAYAEQNEATLQEEIEKEKEFQELLLQLKKHQEEQARRKSSVASVGLLNAEKKNALSENTLSGDGTENMDCNGRTVPKVIIERAPSMEDTSSAQSDQQRNSKVYLEEPTLQKRAESTHSVLSEVALEELEEAHQKCPPWWYKFANTFLIWDCCDLWVKFKEFVRFVVMDPFVDLGITICIVLNTLFMAMEHYPMTEQFEGVLNVGNLVFTGIFAAEMFFKIIALDPYYYFQVGWNIFDSIIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKIASDCELPRWHMHDFFHSFLIVFRILCGEWIETMWDCMEVAGQAMCLVVFMMVMVIGNLVVLNLFLALLLSSFSADNLSASDDDGEMNNLQIAIGRITRGIDFVKKTFFSILHYKRKAKAEQEEEDPNKENIALNHVETEPEPKIELEPRTEPEPKKEPPLIDGVTKKRYYMDEFDHANFFNNPHMRIEVPIASEESDFDIPDTDEFSTQSEVEEGILHHKKYDDGSSVCSTADYKPPEPEEEEEAFEHKEDEGPEECFTADCVRRCPCLYVDITTEKGKIWWNIRKTCFQIVEHNWFETFIIFMILLSSGALAFEDIYIEQRQVIKTILEYADKVFTYIFVFEMLLKWTAYGFKIYFTNAWCWLDFLIVDVSLISLTANWLGYSELGPIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKYYRCINTTTSELFEIDVVNNRSDCLALNNSEQVRWVNVKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSRNIEDQPQYEINLYMYLYFVIFIIFGAFFTLNLFIGVIIDNFNQQKKKFGGKDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPTNKIQGLVYDFVTKQLFDIVIMILICLNMVTMMIETDDQSQEKTDILFKINLVFIVIFTAECVLKMFALRYYFFTIGWNIFDFVVVILSIVGIVLSDLIAEYFVSPTLFRVIRLARIGRVLRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMSNFAYVKKESGIDDIFNFETFGNSIICLFQITTSAGWDGLLNPILNSGPPDCDPHLENFGTTVTGDCGNPAIGIVFFCSYIIISFLIVVNMYIAIILENFNVATEESGEPLCEDDFEMFYETWEKFDPDATQFVEYSRMSDFVDTLQDPLRIPKPNKIKLITLDLPMVTGDKIHCLDILFALTKEVLGDSGEMDALKASMEEKFMMANPSKVSYEPITTTLKRKQEEVCVIKIQRAFRRHLLRRSVKHASYIFRQSNDEESSVEEPPEKEGLIAERINAAYGKYVEEGQNGGTHRPRSTNRTSHSSIDYDDSKSKALAFFLLELTEGTQ

Target ORF information:

RefSeq Version	XM_031894527.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sodium channel, voltage gated, type IV alpha subunit (scn4a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031894527.1

ORF Insert Sequence:

ATGGCTGGCA AGGTGCACCC TCCCGGTCCT GATAGTTTGC GTCCATTTAC CAGAGAGTCC 
TTGGCTGCGA TAGAGAAGCG CATTGAGGAG GACATCGCTC GGAAAGCCAA ACAGATTGAG 
GTGCTCGATG AGAAAGTACC GACACCCAAA AATGGACTGG AGGCAGGAAA GAACTTGCCT 
CTTATATATG GGGATCCCCC ACCCGAACTT ATTGGGATTC CCATAGAGGA ACTAGATTCT 
TTTTACAGCA ACCAAAAGAC ATTTATCGTC TTAAACAAAG GAAAAGCAAT ATTCCGATTC 
ACAGCCACCA AAGCCTTATA TCTACTGGAT CCATTTAATC CAATCAGAAG AGGCGCTATT 
AAAGTCTTAA TCCATTCATT GTTTAGCTTA TTTATTATGT TTACCATCTT AACAAATTGT 
GTTTTTATGA CCATGAGTGA CCCACCACCC TGGTCCAAGA ATGTAGAGTA CACGTTTACT 
GGAATTTACA CCTTTGAGTC CTTAATAAAA ATCCTGGCTC GGGGTTTTTG TATTGACAGT 
TTTACATTCC TCAGGGATCC GTGGAACTGG CTGGATTTCT GTGTCATTGT TATGGCTTAC 
ACCACAGAGT TTGTAGATTT GGGGAATGTG TCTGCCCTTA GGACATTCCG TGTACTCCGT 
GCTCTTAAAA CAATTACAGT TATTCCAGGC CTGAAGACTA TCGTTGGTGC CTTGATCCAA 
TCAGTGAAGA AGCTCGCAGA TGTGATGATC CTCACCCTCT TCTGCCTTGC TGTCTTTGCC 
CTCATTGGCC TTCAGCTCTT TATGGGAAAT CTGAGGCACA AATGTGTCCG ATGGCCACCC 
CCTGATGACT ATTTCATGCT TTATGACAAC AGTACCTTAA ACTCCACACT ATATAATGAT 
ACAATGATCA ACTCCACTCT CAGCAACAGC ACAAATGTGT TCGACAACGA CACCATTGAC 
TGGGGTGCCT ATCTCAACGA TGAAGCAAAT TTTTACTTCC TGGATGGAGC AAATGATGCA 
CTTCTGTGTG GGAACAGCAG TGATGCCGGG AAATGTCCAG AAAGTTACAC ATGTATGAAA 
ACTGGGAGAA ATCCGAATTA TGGGTACACC AGCTACGACT CATTCAACTG GGCTTTCCTT 
TCCCTTTTCC GATTAATGAC ACAGGACTTC TGGGAGAATC TTTTCCAGTT GACACTACGA 
GCAGCTGGTA AAACCTACAT GGTCTTCTTT GTGGTGGTAA TTTTTCTGGG GTCTTTCTAT 
CTGATCAACT TGATCCTTGC TGTGGTGGCC ATGGCATATG CAGAACAGAA TGAAGCCACT 
CTGCAAGAAG AAATAGAAAA GGAGAAAGAG TTCCAGGAGC TTTTGTTGCA GCTAAAGAAA 
CATCAAGAAG AGCAAGCAAG AAGGAAATCC AGCGTTGCCT CAGTTGGACT TCTGAACGCA 
GAAAAGAAAA ATGCTCTTTC AGAGAACACT CTTTCTGGGG ATGGCACAGA AAATATGGAC 
TGCAATGGGC GAACTGTGCC CAAAGTTATA ATTGAACGTG CACCCTCAAT GGAGGATACA 
AGCTCAGCAC AGTCAGACCA ACAGAGGAAT TCTAAAGTTT ATTTGGAGGA GCCAACTCTG 
CAGAAAAGAG CAGAGAGCAC CCACAGTGTC CTATCGGAAG TAGCTCTTGA AGAACTGGAA 
GAGGCTCATC AGAAATGCCC TCCTTGGTGG TACAAGTTTG CTAACACCTT TTTGATTTGG 
GACTGCTGTG ACCTGTGGGT GAAATTTAAG GAATTTGTAA GGTTTGTGGT CATGGATCCC 
TTTGTTGACC TGGGAATCAC CATTTGCATC GTGCTTAACA CCCTTTTTAT GGCCATGGAG 
CACTACCCAA TGACTGAGCA GTTTGAGGGT GTTCTGAATG TAGGAAACTT GGTCTTTACT 
GGCATATTTG CAGCTGAAAT GTTCTTCAAG ATCATAGCCC TGGATCCATA TTACTACTTC 
CAAGTGGGGT GGAACATATT CGACAGCATT ATAGTCACTT TAAGTTTGGT GGAGTTGGGC 
CTTGCAAATG TGGAAGGTCT GTCCGTGCTC AGATCGTTCC GTCTGCTCAG GGTGTTCAAG 
CTAGCCAAAT CATGGCCAAC CCTTAACATG CTTATCAAAA TCATCGGTAA CTCGGTCGGA 
GCACTTGGAA ACCTCACCTT GGTGTTGGCC ATCATTGTCT TCATCTTTGC GGTGGTGGGG 
ATGCAGCTAT TTGGGAAGAG CTACAAGGAA TGTGTGTGCA AGATTGCCTC TGACTGCGAG 
CTACCACGGT GGCACATGCA CGATTTCTTC CACTCTTTTC TCATTGTCTT CCGTATCCTG 
TGCGGGGAGT GGATAGAGAC AATGTGGGAC TGCATGGAAG TGGCAGGGCA AGCAATGTGC 
CTGGTCGTGT TCATGATGGT CATGGTGATT GGTAACCTGG TGGTCCTCAA TCTCTTTTTG 
GCTTTGTTGC TGAGTTCCTT CAGCGCGGAT AACTTGTCGG CTTCGGACGA CGATGGGGAG 
ATGAACAACC TGCAGATCGC CATCGGAAGG ATCACCAGGG GCATTGACTT TGTGAAGAAA 
ACCTTCTTCT CCATCCTACA TTACAAGAGG AAGGCAAAAG CTGAGCAGGA GGAAGAGGAC 
CCTAACAAAG AAAACATTGC TCTGAACCAT GTAGAGACAG AACCAGAACC CAAGATAGAA 
CTAGAACCCA GGACAGAACC AGAACCCAAA AAAGAACCTC CACTGATTGA TGGCGTGACT 
AAAAAACGTT ATTACATGGA TGAGTTTGAC CACGCTAATT TTTTTAACAA CCCACACATG 
AGAATAGAAG TACCTATTGC CTCAGAAGAG TCCGACTTCG ACATTCCAGA CACGGATGAG 
TTTAGCACAC AGTCGGAGGT AGAGGAAGGG ATTTTACACC ACAAGAAGTA CGATGATGGC 
TCGTCAGTGT GTAGCACAGC AGATTATAAA CCTCCTGAAC CTGAAGAGGA AGAGGAGGCC 
TTTGAACACA AAGAAGACGA AGGCCCAGAA GAATGCTTTA CTGCTGACTG TGTACGAAGA 
TGCCCTTGTC TTTATGTCGA CATCACAACA GAAAAAGGGA AAATTTGGTG GAACATTCGG 
AAAACATGTT TCCAGATTGT GGAGCATAAC TGGTTTGAAA CCTTCATCAT CTTCATGATC 
TTACTCAGTA GTGGAGCACT GGCATTTGAG GATATCTACA TTGAACAGAG GCAAGTGATA 
AAGACTATCC TGGAGTACGC TGATAAAGTC TTCACATATA TTTTTGTGTT TGAAATGCTG 
CTAAAGTGGA CAGCGTATGG CTTCAAGATC TATTTCACGA ATGCCTGGTG CTGGCTGGAC 
TTCCTAATTG TTGACGTGTC TTTAATCAGC CTGACAGCCA ACTGGCTGGG CTACTCGGAA 
CTTGGTCCCA TCAAATCCCT TAGAACGCTG AGGGCCCTTA GGCCGTTACG AGCGTTATCA 
AGATTTGAAG GCATGAGGGT GGTTGTAAAC GCCCTTCTGG GAGCCATTCC TTCTATCATG 
AATGTCCTGC TTGTTTGTCT GATCTTCTGG CTCATCTTCA GCATCATGGG TGTTAACCTC 
TTTGCTGGGA AATATTATCG ATGCATCAAC ACGACCACGT CAGAATTATT TGAGATCGAT 
GTGGTCAACA ACCGCTCGGA CTGCCTCGCT TTAAACAACT CCGAGCAAGT GCGATGGGTT 
AATGTCAAAG TGAATTTTGA CAATGTGGGC CTTGGGTATC TCTCCCTTCT ACAAGTGGCA 
ACCTTCAAAG GCTGGATGGA TATCATGTAT GCCGCTGTAG ATTCCCGGAA TATTGAGGAT 
CAGCCACAAT ATGAAATAAA TCTTTATATG TATTTGTACT TTGTAATATT CATTATTTTT 
GGAGCTTTCT TCACTCTAAA CCTGTTTATC GGTGTCATTA TCGACAACTT CAATCAGCAA 
AAGAAAAAGT TTGGAGGTAA GGATATCTTT ATGACTGAGG AACAGAAGAA ATACTACAAT 
GCTATGAAAA AGCTGGGATC CAAAAAACCC CAAAAGCCAA TTCCAAGACC CACGAACAAA 
ATTCAAGGTT TGGTGTATGA CTTTGTCACA AAGCAGCTCT TTGACATTGT GATTATGATT 
TTAATTTGCC TCAACATGGT GACGATGATG ATTGAAACAG ATGACCAGAG TCAGGAAAAA 
ACTGATATTC TTTTCAAGAT CAATCTGGTG TTCATTGTCA TCTTCACAGC CGAATGTGTT 
CTCAAAATGT TTGCGCTGAG GTACTACTTT TTCACAATAG GATGGAACAT ATTTGACTTT 
GTGGTGGTCA TCCTATCTAT TGTTGGTATT GTTCTCTCGG ATCTCATAGC AGAGTACTTT 
GTTTCACCAA CACTTTTCAG AGTCATCAGA CTGGCGAGAA TCGGTAGAGT TCTTCGACTC 
ATAAGAGGAG CAAAGGGGAT TCGCACATTA CTTTTTGCCC TGATGATGTC TCTCCCCGCT 
CTCTTTAATA TCGGGCTTTT GCTTTTCTTA GTTATGTTCA TCTATTCAAT CTTTGGAATG 
TCCAATTTTG CCTATGTAAA GAAAGAATCT GGAATAGATG ACATATTTAA TTTTGAAACC 
TTTGGAAACA GCATAATATG CTTGTTCCAA ATCACCACGT CTGCTGGTTG GGATGGTCTT 
TTAAATCCAA TCCTAAATAG CGGCCCACCA GATTGTGACC CCCATCTGGA GAACTTTGGA 
ACAACAGTTA CTGGTGATTG TGGTAACCCA GCTATAGGTA TTGTGTTCTT CTGCAGCTAC 
ATCATAATAT CATTTTTAAT AGTTGTCAAT ATGTACATTG CCATTATCCT GGAGAACTTT 
AATGTTGCCA CAGAAGAGAG TGGTGAGCCA TTATGTGAGG ATGACTTTGA GATGTTTTAT 
GAAACTTGGG AGAAGTTTGA TCCAGATGCC ACTCAGTTTG TTGAATACAG TCGAATGTCA 
GACTTTGTAG ACACTTTGCA AGATCCTCTA CGGATTCCGA AACCTAACAA AATAAAACTA 
ATTACTTTGG ATCTCCCAAT GGTAACTGGA GATAAGATTC ACTGCTTGGA TATTCTGTTT 
GCACTAACTA AAGAGGTGTT GGGGGACTCA GGAGAAATGG ATGCATTAAA AGCCTCAATG 
GAAGAAAAGT TTATGATGGC AAATCCATCA AAAGTGTCTT ATGAACCAAT CACCACCACC 
CTTAAAAGAA AGCAGGAAGA AGTCTGTGTC ATCAAAATCC AGAGGGCCTT TAGGAGACAT 
CTATTGAGAC GTTCGGTGAA ACACGCTTCC TACATCTTCA GGCAAAGTAA TGATGAAGAG 
TCATCAGTGG AGGAACCTCC TGAAAAAGAA GGGTTGATAG CTGAAAGAAT TAATGCAGCC 
TACGGAAAAT ATGTAGAGGA AGGGCAAAAC GGCGGAACTC ATCGCCCAAG ATCAACAAAC 
AGAACTTCAC ACAGCAGTAT AGATTATGAT GACTCCAAAT CAAAAGCTCT TGCCTTCTTC 
CTGTTGGAAC TGACAGAGGG AACACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scn4a ( XM_031894528.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031750388.1 Xenopus tropicalis sodium channel, voltage gated, type IV alpha subunit (scn4a), transcript variant X5, mRNA.

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CloneID	OXa50619
Clone ID Related Accession (Same CDS sequence)	XM_031894528.1
Accession Version	XM_031894528.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sodium channel protein type 4 subunit alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGCA AGGTGCACCC TCCCGGTCCT GATAGTTTGC GTCCATTTAC CAGAGAGTCC 
TTGGCTGCGA TAGAGAAGCG CATTGAGGAG GACATCGCTC GGAAAGCCAA ACAGATTGAG 
GTGCTCGATG AGAAAGTACC GACACCCAAA AATGGACTGG AGGCAGGAAA GAACTTGCCT 
CTTATATATG GGGATCCCCC ACCCGAACTT ATTGGGATTC CCATAGAGGA ACTAGATTCT 
TTTTACAGCA ACCAAAAGAC ATTTATCGTC TTAAACAAAG GAAAAGCAAT ATTCCGATTC 
ACAGCCACCA AAGCCTTATA TCTACTGGAT CCATTTAATC CAATCAGAAG AGGCGCTATT 
AAAGTCTTAA TCCATTCATT GTTTAGCTTA TTTATTATGT TTACCATCTT AACAAATTGT 
GTTTTTATGA CCATGAGTGA CCCACCACCC TGGTCCAAGA ATGTAGAGTA CACGTTTACT 
GGAATTTACA CCTTTGAGTC CTTAATAAAA ATCCTGGCTC GGGGTTTTTG TATTGACAGT 
TTTACATTCC TCAGGGATCC GTGGAACTGG CTGGATTTCT GTGTCATTGT TATGGCTTAC 
ACCACAGAGT TTGTAGATTT GGGGAATGTG TCTGCCCTTA GGACATTCCG TGTACTCCGT 
GCTCTTAAAA CAATTACAGT TATTCCAGGC CTGAAGACTA TCGTTGGTGC CTTGATCCAA 
TCAGTGAAGA AGCTCGCAGA TGTGATGATC CTCACCCTCT TCTGCCTTGC TGTCTTTGCC 
CTCATTGGCC TTCAGCTCTT TATGGGAAAT CTGAGGCACA AATGTGTCCG ATGGCCACCC 
CCTGATGACT ATTTCATGCT TTATGACAAC AGTACCTTAA ACTCCACACT ATATAATGAT 
ACAATGATCA ACTCCACTCT CAGCAACAGC ACAAATGTGT TCGACAACGA CACCATTGAC 
TGGGGTGCCT ATCTCAACGA TGAAGCAAAT TTTTACTTCC TGGATGGAGC AAATGATGCA 
CTTCTGTGTG GGAACAGCAG TGATGCCGGG AAATGTCCAG AAAGTTACAC ATGTATGAAA 
ACTGGGAGAA ATCCGAATTA TGGGTACACC AGCTACGACT CATTCAACTG GGCTTTCCTT 
TCCCTTTTCC GATTAATGAC ACAGGACTTC TGGGAGAATC TTTTCCAGTT GACACTACGA 
GCAGCTGGTA AAACCTACAT GGTCTTCTTT GTGGTGGTAA TTTTTCTGGG GTCTTTCTAT 
CTGATCAACT TGATCCTTGC TGTGGTGGCC ATGGCATATG CAGAACAGAA TGAAGCCACT 
CTGCAAGAAG AAATAGAAAA GGAGAAAGAG TTCCAGGAGC TTTTGTTGCA GCTAAAGAAA 
CATCAAGAAG AGCAAGCAAG AAGGAAATCC AGCGTTGCCT CAGTTGGACT TCTGAACGCA 
GAAAAGAAAA ATGCTCTTTC AGAGAACACT CTTTCTGGGG ATGGCACAGA AAATATGGAC 
TGCAATGGGC GAACTGTGCC CAAAGTTATA ATTGAACGTG CACCCTCAAT GGAGGATACA 
AGCTCAGCAC AGTCAGACCA ACAGAGGAAT TCTAAAGTTT ATTTGGAGGA GCCAACTCTG 
CAGAAAAGAG CAGAGAGCAC CCACAGTGTC CTATCGGAAG TAGCTCTTGA AGAACTGGAA 
GAGGCTCATC AGAAATGCCC TCCTTGGTGG TACAAGTTTG CTAACACCTT TTTGATTTGG 
GACTGCTGTG ACCTGTGGGT GAAATTTAAG GAATTTGTAA GGTTTGTGGT CATGGATCCC 
TTTGTTGACC TGGGAATCAC CATTTGCATC GTGCTTAACA CCCTTTTTAT GGCCATGGAG 
CACTACCCAA TGACTGAGCA GTTTGAGGGT GTTCTGAATG TAGGAAACTT GGTCTTTACT 
GGCATATTTG CAGCTGAAAT GTTCTTCAAG ATCATAGCCC TGGATCCATA TTACTACTTC 
CAAGTGGGGT GGAACATATT CGACAGCATT ATAGTCACTT TAAGTTTGGT GGAGTTGGGC 
CTTGCAAATG TGGAAGGTCT GTCCGTGCTC AGATCGTTCC GTCTGCTCAG GGTGTTCAAG 
CTAGCCAAAT CATGGCCAAC CCTTAACATG CTTATCAAAA TCATCGGTAA CTCGGTCGGA 
GCACTTGGAA ACCTCACCTT GGTGTTGGCC ATCATTGTCT TCATCTTTGC GGTGGTGGGG 
ATGCAGCTAT TTGGGAAGAG CTACAAGGAA TGTGTGTGCA AGATTGCCTC TGACTGCGAG 
CTACCACGGT GGCACATGCA CGATTTCTTC CACTCTTTTC TCATTGTCTT CCGTATCCTG 
TGCGGGGAGT GGATAGAGAC AATGTGGGAC TGCATGGAAG TGGCAGGGCA AGCAATGTGC 
CTGGTCGTGT TCATGATGGT CATGGTGATT GGTAACCTGG TGGTCCTCAA TCTCTTTTTG 
GCTTTGTTGC TGAGTTCCTT CAGCGCGGAT AACTTGTCGG CTTCGGACGA CGATGGGGAG 
ATGAACAACC TGCAGATCGC CATCGGAAGG ATCACCAGGG GCATTGACTT TGTGAAGAAA 
ACCTTCTTCT CCATCCTACA TTACAAGAGG AAGGCAAAAG CTGAGCAGGA GGAAGAGGAC 
CCTAACAAAG AAAACATTGC TCTGAACCAT GTAGAGACAG AACCAGAACC CAAGATAGAA 
CTAGAACCCA GGACAGAACC AGAACCCAAA AAAGAACCTC CACTGATTGA TGGCGTGACT 
AAAAAACGTT ATTACATGGA TGAGTTTGAC CACGCTAATT TTTTTAACAA CCCACACATG 
AGAATAGAAG TACCTATTGC CTCAGAAGAG TCCGACTTCG ACATTCCAGA CACGGATGAG 
TTTAGCACAC AGTCGGAGGT AGAGGAAGGG ATTTTACACC ACAAGAAGTA CGATGATGGC 
TCGTCAGTGT GTAGCACAGC AGATTATAAA CCTCCTGAAC CTGAAGAGGA AGAGGAGGCC 
TTTGAACACA AAGAAGACGA AGGCCCAGAA GAATGCTTTA CTGCTGACTG TGTACGAAGA 
TGCCCTTGTC TTTATGTCGA CATCACAACA GAAAAAGGGA AAATTTGGTG GAACATTCGG 
AAAACATGTT TCCAGATTGT GGAGCATAAC TGGTTTGAAA CCTTCATCAT CTTCATGATC 
TTACTCAGTA GTGGAGCACT GGCATTTGAG GATATCTACA TTGAACAGAG GCAAGTGATA 
AAGACTATCC TGGAGTACGC TGATAAAGTC TTCACATATA TTTTTGTGTT TGAAATGCTG 
CTAAAGTGGA CAGCGTATGG CTTCAAGATC TATTTCACGA ATGCCTGGTG CTGGCTGGAC 
TTCCTAATTG TTGACGTGTC TTTAATCAGC CTGACAGCCA ACTGGCTGGG CTACTCGGAA 
CTTGGTCCCA TCAAATCCCT TAGAACGCTG AGGGCCCTTA GGCCGTTACG AGCGTTATCA 
AGATTTGAAG GCATGAGGGT GGTTGTAAAC GCCCTTCTGG GAGCCATTCC TTCTATCATG 
AATGTCCTGC TTGTTTGTCT GATCTTCTGG CTCATCTTCA GCATCATGGG TGTTAACCTC 
TTTGCTGGGA AATATTATCG ATGCATCAAC ACGACCACGT CAGAATTATT TGAGATCGAT 
GTGGTCAACA ACCGCTCGGA CTGCCTCGCT TTAAACAACT CCGAGCAAGT GCGATGGGTT 
AATGTCAAAG TGAATTTTGA CAATGTGGGC CTTGGGTATC TCTCCCTTCT ACAAGTGGCA 
ACCTTCAAAG GCTGGATGGA TATCATGTAT GCCGCTGTAG ATTCCCGGAA TATTGAGGAT 
CAGCCACAAT ATGAAATAAA TCTTTATATG TATTTGTACT TTGTAATATT CATTATTTTT 
GGAGCTTTCT TCACTCTAAA CCTGTTTATC GGTGTCATTA TCGACAACTT CAATCAGCAA 
AAGAAAAAGT TTGGAGGTAA GGATATCTTT ATGACTGAGG AACAGAAGAA ATACTACAAT 
GCTATGAAAA AGCTGGGATC CAAAAAACCC CAAAAGCCAA TTCCAAGACC CACGAACAAA 
ATTCAAGGTT TGGTGTATGA CTTTGTCACA AAGCAGCTCT TTGACATTGT GATTATGATT 
TTAATTTGCC TCAACATGGT GACGATGATG ATTGAAACAG ATGACCAGAG TCAGGAAAAA 
ACTGATATTC TTTTCAAGAT CAATCTGGTG TTCATTGTCA TCTTCACAGC CGAATGTGTT 
CTCAAAATGT TTGCGCTGAG GTACTACTTT TTCACAATAG GATGGAACAT ATTTGACTTT 
GTGGTGGTCA TCCTATCTAT TGTTGGTATT GTTCTCTCGG ATCTCATAGC AGAGTACTTT 
GTTTCACCAA CACTTTTCAG AGTCATCAGA CTGGCGAGAA TCGGTAGAGT TCTTCGACTC 
ATAAGAGGAG CAAAGGGGAT TCGCACATTA CTTTTTGCCC TGATGATGTC TCTCCCCGCT 
CTCTTTAATA TCGGGCTTTT GCTTTTCTTA GTTATGTTCA TCTATTCAAT CTTTGGAATG 
TCCAATTTTG CCTATGTAAA GAAAGAATCT GGAATAGATG ACATATTTAA TTTTGAAACC 
TTTGGAAACA GCATAATATG CTTGTTCCAA ATCACCACGT CTGCTGGTTG GGATGGTCTT 
TTAAATCCAA TCCTAAATAG CGGCCCACCA GATTGTGACC CCCATCTGGA GAACTTTGGA 
ACAACAGTTA CTGGTGATTG TGGTAACCCA GCTATAGGTA TTGTGTTCTT CTGCAGCTAC 
ATCATAATAT CATTTTTAAT AGTTGTCAAT ATGTACATTG CCATTATCCT GGAGAACTTT 
AATGTTGCCA CAGAAGAGAG TGGTGAGCCA TTATGTGAGG ATGACTTTGA GATGTTTTAT 
GAAACTTGGG AGAAGTTTGA TCCAGATGCC ACTCAGTTTG TTGAATACAG TCGAATGTCA 
GACTTTGTAG ACACTTTGCA AGATCCTCTA CGGATTCCGA AACCTAACAA AATAAAACTA 
ATTACTTTGG ATCTCCCAAT GGTAACTGGA GATAAGATTC ACTGCTTGGA TATTCTGTTT 
GCACTAACTA AAGAGGTGTT GGGGGACTCA GGAGAAATGG ATGCATTAAA AGCCTCAATG 
GAAGAAAAGT TTATGATGGC AAATCCATCA AAAGTGTCTT ATGAACCAAT CACCACCACC 
CTTAAAAGAA AGCAGGAAGA AGTCTGTGTC ATCAAAATCC AGAGGGCCTT TAGGAGACAT 
CTATTGAGAC GTTCGGTGAA ACACGCTTCC TACATCTTCA GGCAAAGTAA TGATGAAGAG 
TCATCAGTGG AGGAACCTCC TGAAAAAGAA GGGTTGATAG CTGAAAGAAT TAATGCAGCC 
TACGGAAAAT ATGTAGAGGA AGGGCAAAAC GGCGGAACTC ATCGCCCAAG ATCAACAAAC 
AGAACTTCAC ACAGCAGTAT AGATTATGAT GTAAGAGAGA CAGATGTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031750388.1
CDS	158..5668
Translation	MAGKVHPPGPDSLRPFTRESLAAIEKRIEEDIARKAKQIEVLDEKVPTPKNGLEAGKNLPLIYGDPPPELIGIPIEELDSFYSNQKTFIVLNKGKAIFRFTATKALYLLDPFNPIRRGAIKVLIHSLFSLFIMFTILTNCVFMTMSDPPPWSKNVEYTFTGIYTFESLIKILARGFCIDSFTFLRDPWNWLDFCVIVMAYTTEFVDLGNVSALRTFRVLRALKTITVIPGLKTIVGALIQSVKKLADVMILTLFCLAVFALIGLQLFMGNLRHKCVRWPPPDDYFMLYDNSTLNSTLYNDTMINSTLSNSTNVFDNDTIDWGAYLNDEANFYFLDGANDALLCGNSSDAGKCPESYTCMKTGRNPNYGYTSYDSFNWAFLSLFRLMTQDFWENLFQLTLRAAGKTYMVFFVVVIFLGSFYLINLILAVVAMAYAEQNEATLQEEIEKEKEFQELLLQLKKHQEEQARRKSSVASVGLLNAEKKNALSENTLSGDGTENMDCNGRTVPKVIIERAPSMEDTSSAQSDQQRNSKVYLEEPTLQKRAESTHSVLSEVALEELEEAHQKCPPWWYKFANTFLIWDCCDLWVKFKEFVRFVVMDPFVDLGITICIVLNTLFMAMEHYPMTEQFEGVLNVGNLVFTGIFAAEMFFKIIALDPYYYFQVGWNIFDSIIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKIASDCELPRWHMHDFFHSFLIVFRILCGEWIETMWDCMEVAGQAMCLVVFMMVMVIGNLVVLNLFLALLLSSFSADNLSASDDDGEMNNLQIAIGRITRGIDFVKKTFFSILHYKRKAKAEQEEEDPNKENIALNHVETEPEPKIELEPRTEPEPKKEPPLIDGVTKKRYYMDEFDHANFFNNPHMRIEVPIASEESDFDIPDTDEFSTQSEVEEGILHHKKYDDGSSVCSTADYKPPEPEEEEEAFEHKEDEGPEECFTADCVRRCPCLYVDITTEKGKIWWNIRKTCFQIVEHNWFETFIIFMILLSSGALAFEDIYIEQRQVIKTILEYADKVFTYIFVFEMLLKWTAYGFKIYFTNAWCWLDFLIVDVSLISLTANWLGYSELGPIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKYYRCINTTTSELFEIDVVNNRSDCLALNNSEQVRWVNVKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSRNIEDQPQYEINLYMYLYFVIFIIFGAFFTLNLFIGVIIDNFNQQKKKFGGKDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPTNKIQGLVYDFVTKQLFDIVIMILICLNMVTMMIETDDQSQEKTDILFKINLVFIVIFTAECVLKMFALRYYFFTIGWNIFDFVVVILSIVGIVLSDLIAEYFVSPTLFRVIRLARIGRVLRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMSNFAYVKKESGIDDIFNFETFGNSIICLFQITTSAGWDGLLNPILNSGPPDCDPHLENFGTTVTGDCGNPAIGIVFFCSYIIISFLIVVNMYIAIILENFNVATEESGEPLCEDDFEMFYETWEKFDPDATQFVEYSRMSDFVDTLQDPLRIPKPNKIKLITLDLPMVTGDKIHCLDILFALTKEVLGDSGEMDALKASMEEKFMMANPSKVSYEPITTTLKRKQEEVCVIKIQRAFRRHLLRRSVKHASYIFRQSNDEESSVEEPPEKEGLIAERINAAYGKYVEEGQNGGTHRPRSTNRTSHSSIDYDVRETDV

Target ORF information:

RefSeq Version	XM_031894528.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sodium channel, voltage gated, type IV alpha subunit (scn4a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031894528.1

ORF Insert Sequence:

ATGGCTGGCA AGGTGCACCC TCCCGGTCCT GATAGTTTGC GTCCATTTAC CAGAGAGTCC 
TTGGCTGCGA TAGAGAAGCG CATTGAGGAG GACATCGCTC GGAAAGCCAA ACAGATTGAG 
GTGCTCGATG AGAAAGTACC GACACCCAAA AATGGACTGG AGGCAGGAAA GAACTTGCCT 
CTTATATATG GGGATCCCCC ACCCGAACTT ATTGGGATTC CCATAGAGGA ACTAGATTCT 
TTTTACAGCA ACCAAAAGAC ATTTATCGTC TTAAACAAAG GAAAAGCAAT ATTCCGATTC 
ACAGCCACCA AAGCCTTATA TCTACTGGAT CCATTTAATC CAATCAGAAG AGGCGCTATT 
AAAGTCTTAA TCCATTCATT GTTTAGCTTA TTTATTATGT TTACCATCTT AACAAATTGT 
GTTTTTATGA CCATGAGTGA CCCACCACCC TGGTCCAAGA ATGTAGAGTA CACGTTTACT 
GGAATTTACA CCTTTGAGTC CTTAATAAAA ATCCTGGCTC GGGGTTTTTG TATTGACAGT 
TTTACATTCC TCAGGGATCC GTGGAACTGG CTGGATTTCT GTGTCATTGT TATGGCTTAC 
ACCACAGAGT TTGTAGATTT GGGGAATGTG TCTGCCCTTA GGACATTCCG TGTACTCCGT 
GCTCTTAAAA CAATTACAGT TATTCCAGGC CTGAAGACTA TCGTTGGTGC CTTGATCCAA 
TCAGTGAAGA AGCTCGCAGA TGTGATGATC CTCACCCTCT TCTGCCTTGC TGTCTTTGCC 
CTCATTGGCC TTCAGCTCTT TATGGGAAAT CTGAGGCACA AATGTGTCCG ATGGCCACCC 
CCTGATGACT ATTTCATGCT TTATGACAAC AGTACCTTAA ACTCCACACT ATATAATGAT 
ACAATGATCA ACTCCACTCT CAGCAACAGC ACAAATGTGT TCGACAACGA CACCATTGAC 
TGGGGTGCCT ATCTCAACGA TGAAGCAAAT TTTTACTTCC TGGATGGAGC AAATGATGCA 
CTTCTGTGTG GGAACAGCAG TGATGCCGGG AAATGTCCAG AAAGTTACAC ATGTATGAAA 
ACTGGGAGAA ATCCGAATTA TGGGTACACC AGCTACGACT CATTCAACTG GGCTTTCCTT 
TCCCTTTTCC GATTAATGAC ACAGGACTTC TGGGAGAATC TTTTCCAGTT GACACTACGA 
GCAGCTGGTA AAACCTACAT GGTCTTCTTT GTGGTGGTAA TTTTTCTGGG GTCTTTCTAT 
CTGATCAACT TGATCCTTGC TGTGGTGGCC ATGGCATATG CAGAACAGAA TGAAGCCACT 
CTGCAAGAAG AAATAGAAAA GGAGAAAGAG TTCCAGGAGC TTTTGTTGCA GCTAAAGAAA 
CATCAAGAAG AGCAAGCAAG AAGGAAATCC AGCGTTGCCT CAGTTGGACT TCTGAACGCA 
GAAAAGAAAA ATGCTCTTTC AGAGAACACT CTTTCTGGGG ATGGCACAGA AAATATGGAC 
TGCAATGGGC GAACTGTGCC CAAAGTTATA ATTGAACGTG CACCCTCAAT GGAGGATACA 
AGCTCAGCAC AGTCAGACCA ACAGAGGAAT TCTAAAGTTT ATTTGGAGGA GCCAACTCTG 
CAGAAAAGAG CAGAGAGCAC CCACAGTGTC CTATCGGAAG TAGCTCTTGA AGAACTGGAA 
GAGGCTCATC AGAAATGCCC TCCTTGGTGG TACAAGTTTG CTAACACCTT TTTGATTTGG 
GACTGCTGTG ACCTGTGGGT GAAATTTAAG GAATTTGTAA GGTTTGTGGT CATGGATCCC 
TTTGTTGACC TGGGAATCAC CATTTGCATC GTGCTTAACA CCCTTTTTAT GGCCATGGAG 
CACTACCCAA TGACTGAGCA GTTTGAGGGT GTTCTGAATG TAGGAAACTT GGTCTTTACT 
GGCATATTTG CAGCTGAAAT GTTCTTCAAG ATCATAGCCC TGGATCCATA TTACTACTTC 
CAAGTGGGGT GGAACATATT CGACAGCATT ATAGTCACTT TAAGTTTGGT GGAGTTGGGC 
CTTGCAAATG TGGAAGGTCT GTCCGTGCTC AGATCGTTCC GTCTGCTCAG GGTGTTCAAG 
CTAGCCAAAT CATGGCCAAC CCTTAACATG CTTATCAAAA TCATCGGTAA CTCGGTCGGA 
GCACTTGGAA ACCTCACCTT GGTGTTGGCC ATCATTGTCT TCATCTTTGC GGTGGTGGGG 
ATGCAGCTAT TTGGGAAGAG CTACAAGGAA TGTGTGTGCA AGATTGCCTC TGACTGCGAG 
CTACCACGGT GGCACATGCA CGATTTCTTC CACTCTTTTC TCATTGTCTT CCGTATCCTG 
TGCGGGGAGT GGATAGAGAC AATGTGGGAC TGCATGGAAG TGGCAGGGCA AGCAATGTGC 
CTGGTCGTGT TCATGATGGT CATGGTGATT GGTAACCTGG TGGTCCTCAA TCTCTTTTTG 
GCTTTGTTGC TGAGTTCCTT CAGCGCGGAT AACTTGTCGG CTTCGGACGA CGATGGGGAG 
ATGAACAACC TGCAGATCGC CATCGGAAGG ATCACCAGGG GCATTGACTT TGTGAAGAAA 
ACCTTCTTCT CCATCCTACA TTACAAGAGG AAGGCAAAAG CTGAGCAGGA GGAAGAGGAC 
CCTAACAAAG AAAACATTGC TCTGAACCAT GTAGAGACAG AACCAGAACC CAAGATAGAA 
CTAGAACCCA GGACAGAACC AGAACCCAAA AAAGAACCTC CACTGATTGA TGGCGTGACT 
AAAAAACGTT ATTACATGGA TGAGTTTGAC CACGCTAATT TTTTTAACAA CCCACACATG 
AGAATAGAAG TACCTATTGC CTCAGAAGAG TCCGACTTCG ACATTCCAGA CACGGATGAG 
TTTAGCACAC AGTCGGAGGT AGAGGAAGGG ATTTTACACC ACAAGAAGTA CGATGATGGC 
TCGTCAGTGT GTAGCACAGC AGATTATAAA CCTCCTGAAC CTGAAGAGGA AGAGGAGGCC 
TTTGAACACA AAGAAGACGA AGGCCCAGAA GAATGCTTTA CTGCTGACTG TGTACGAAGA 
TGCCCTTGTC TTTATGTCGA CATCACAACA GAAAAAGGGA AAATTTGGTG GAACATTCGG 
AAAACATGTT TCCAGATTGT GGAGCATAAC TGGTTTGAAA CCTTCATCAT CTTCATGATC 
TTACTCAGTA GTGGAGCACT GGCATTTGAG GATATCTACA TTGAACAGAG GCAAGTGATA 
AAGACTATCC TGGAGTACGC TGATAAAGTC TTCACATATA TTTTTGTGTT TGAAATGCTG 
CTAAAGTGGA CAGCGTATGG CTTCAAGATC TATTTCACGA ATGCCTGGTG CTGGCTGGAC 
TTCCTAATTG TTGACGTGTC TTTAATCAGC CTGACAGCCA ACTGGCTGGG CTACTCGGAA 
CTTGGTCCCA TCAAATCCCT TAGAACGCTG AGGGCCCTTA GGCCGTTACG AGCGTTATCA 
AGATTTGAAG GCATGAGGGT GGTTGTAAAC GCCCTTCTGG GAGCCATTCC TTCTATCATG 
AATGTCCTGC TTGTTTGTCT GATCTTCTGG CTCATCTTCA GCATCATGGG TGTTAACCTC 
TTTGCTGGGA AATATTATCG ATGCATCAAC ACGACCACGT CAGAATTATT TGAGATCGAT 
GTGGTCAACA ACCGCTCGGA CTGCCTCGCT TTAAACAACT CCGAGCAAGT GCGATGGGTT 
AATGTCAAAG TGAATTTTGA CAATGTGGGC CTTGGGTATC TCTCCCTTCT ACAAGTGGCA 
ACCTTCAAAG GCTGGATGGA TATCATGTAT GCCGCTGTAG ATTCCCGGAA TATTGAGGAT 
CAGCCACAAT ATGAAATAAA TCTTTATATG TATTTGTACT TTGTAATATT CATTATTTTT 
GGAGCTTTCT TCACTCTAAA CCTGTTTATC GGTGTCATTA TCGACAACTT CAATCAGCAA 
AAGAAAAAGT TTGGAGGTAA GGATATCTTT ATGACTGAGG AACAGAAGAA ATACTACAAT 
GCTATGAAAA AGCTGGGATC CAAAAAACCC CAAAAGCCAA TTCCAAGACC CACGAACAAA 
ATTCAAGGTT TGGTGTATGA CTTTGTCACA AAGCAGCTCT TTGACATTGT GATTATGATT 
TTAATTTGCC TCAACATGGT GACGATGATG ATTGAAACAG ATGACCAGAG TCAGGAAAAA 
ACTGATATTC TTTTCAAGAT CAATCTGGTG TTCATTGTCA TCTTCACAGC CGAATGTGTT 
CTCAAAATGT TTGCGCTGAG GTACTACTTT TTCACAATAG GATGGAACAT ATTTGACTTT 
GTGGTGGTCA TCCTATCTAT TGTTGGTATT GTTCTCTCGG ATCTCATAGC AGAGTACTTT 
GTTTCACCAA CACTTTTCAG AGTCATCAGA CTGGCGAGAA TCGGTAGAGT TCTTCGACTC 
ATAAGAGGAG CAAAGGGGAT TCGCACATTA CTTTTTGCCC TGATGATGTC TCTCCCCGCT 
CTCTTTAATA TCGGGCTTTT GCTTTTCTTA GTTATGTTCA TCTATTCAAT CTTTGGAATG 
TCCAATTTTG CCTATGTAAA GAAAGAATCT GGAATAGATG ACATATTTAA TTTTGAAACC 
TTTGGAAACA GCATAATATG CTTGTTCCAA ATCACCACGT CTGCTGGTTG GGATGGTCTT 
TTAAATCCAA TCCTAAATAG CGGCCCACCA GATTGTGACC CCCATCTGGA GAACTTTGGA 
ACAACAGTTA CTGGTGATTG TGGTAACCCA GCTATAGGTA TTGTGTTCTT CTGCAGCTAC 
ATCATAATAT CATTTTTAAT AGTTGTCAAT ATGTACATTG CCATTATCCT GGAGAACTTT 
AATGTTGCCA CAGAAGAGAG TGGTGAGCCA TTATGTGAGG ATGACTTTGA GATGTTTTAT 
GAAACTTGGG AGAAGTTTGA TCCAGATGCC ACTCAGTTTG TTGAATACAG TCGAATGTCA 
GACTTTGTAG ACACTTTGCA AGATCCTCTA CGGATTCCGA AACCTAACAA AATAAAACTA 
ATTACTTTGG ATCTCCCAAT GGTAACTGGA GATAAGATTC ACTGCTTGGA TATTCTGTTT 
GCACTAACTA AAGAGGTGTT GGGGGACTCA GGAGAAATGG ATGCATTAAA AGCCTCAATG 
GAAGAAAAGT TTATGATGGC AAATCCATCA AAAGTGTCTT ATGAACCAAT CACCACCACC 
CTTAAAAGAA AGCAGGAAGA AGTCTGTGTC ATCAAAATCC AGAGGGCCTT TAGGAGACAT 
CTATTGAGAC GTTCGGTGAA ACACGCTTCC TACATCTTCA GGCAAAGTAA TGATGAAGAG 
TCATCAGTGG AGGAACCTCC TGAAAAAGAA GGGTTGATAG CTGAAAGAAT TAATGCAGCC 
TACGGAAAAT ATGTAGAGGA AGGGCAAAAC GGCGGAACTC ATCGCCCAAG ATCAACAAAC 
AGAACTTCAC ACAGCAGTAT AGATTATGAT GTAAGAGAGA CAGATGTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scn4a ( XM_031894530.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031750390.1 Xenopus tropicalis sodium channel, voltage gated, type IV alpha subunit (scn4a), transcript variant X7, mRNA.

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CloneID	OXa50620
Clone ID Related Accession (Same CDS sequence)	XM_031894530.1 , XM_031894529.1
Accession Version	XM_031894530.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sodium channel protein type 4 subunit alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGCA AGGTGCACCC TCCCGGTCCT GATAGTTTGC GTCCATTTAC CAGAGAGTCC 
TTGGCTGCGA TAGAGAAGCG CATTGAGGAG GACATCGCTC GGAAAGCCAA ACAGATTGAG 
GTGCTCGATG AGAAAGTACC GACACCCAAA AATGGACTGG AGGCAGGAAA GAACTTGCCT 
CTTATATATG GGGATCCCCC ACCCGAACTT ATTGGGATTC CCATAGAGGA ACTAGATTCT 
TTTTACAGCA ACCAAAAGAC ATTTATCGTC TTAAACAAAG GAAAAGCAAT ATTCCGATTC 
ACAGCCACCA AAGCCTTATA TCTACTGGAT CCATTTAATC CAATCAGAAG AGGCGCTATT 
AAAGTCTTAA TCCATTCATT GTTTAGCTTA TTTATTATGT TTACCATCTT AACAAATTGT 
GTTTTTATGA CCATGAGTGA CCCACCACCC TGGTCCAAGA ATGTAGAGTA CACGTTTACT 
GGAATTTACA CCTTTGAGTC CTTAATAAAA ATCCTGGCTC GGGGTTTTTG TATTGACAGT 
TTTACATTCC TCAGGGATCC GTGGAACTGG CTGGATTTCT GTGTCATTGT TATGGCTTAC 
ACCACAGAGT TTGTAGATTT GGGGAATGTG TCTGCCCTTA GGACATTCCG TGTACTCCGT 
GCTCTTAAAA CAATTACAGT TATTCCAGGC CTGAAGACTA TCGTTGGTGC CTTGATCCAA 
TCAGTGAAGA AGCTCGCAGA TGTGATGATC CTCACCCTCT TCTGCCTTGC TGTCTTTGCC 
CTCATTGGCC TTCAGCTCTT TATGGGAAAT CTGAGGCACA AATGTGTCCG ATGGCCACCC 
CCTGATGACT ATTTCATGCT TTATGACAAC AGTACCTTAA ACTCCACACT ATATAATGAT 
ACAATGATCA ACTCCACTCT CAGCAACAGC ACAAATGTGT TCGACAACGA CACCATTGAC 
TGGGGTGCCT ATCTCAACGA TGAAGCAAAT TTTTACTTCC TGGATGGAGC AAATGATGCA 
CTTCTGTGTG GGAACAGCAG TGATGCCGGG AAATGTCCAG AAAGTTACAC ATGTATGAAA 
ACTGGGAGAA ATCCGAATTA TGGGTACACC AGCTACGACT CATTCAACTG GGCTTTCCTT 
TCCCTTTTCC GATTAATGAC ACAGGACTTC TGGGAGAATC TTTTCCAGTT GACACTACGA 
GCAGCTGGTA AAACCTACAT GGTCTTCTTT GTGGTGGTAA TTTTTCTGGG GTCTTTCTAT 
CTGATCAACT TGATCCTTGC TGTGGTGGCC ATGGCATATG CAGAACAGAA TGAAGCCACT 
CTGCAAGAAG AAATAGAAAA GGAGAAAGAG TTCCAGGAGC TTTTGTTGCA GCTAAAGAAA 
CATCAAGAAG AGCAAGCAAG AAGGAAATCC AGCGTTGCCT CAGTTGGACT TCTGAACGCA 
GAAAAGAAAA ATGCTCTTTC AGAGAACACT CTTTCTGGGG ATGGCACAGA AAATATGGAC 
TGCAATGGGC GAACTGTGCC CAAAGTTATA ATTGAACGTG CACCCTCAAT GGAGGATACA 
AGCTCAGCAC AGTCAGACCA ACAGAGGAAT TCTAAAGTTT ATTTGGAGGA GCCAACTCTG 
CAGAAAAGAG CAGAGAGCAC CCACAGTGTC CTATCGGAAG TAGCTCTTGA AGAACTGGAA 
GAGGCTCATC AGAAATGCCC TCCTTGGTGG TACAAGTTTG CTAACACCTT TTTGATTTGG 
GACTGCTGTG ACCTGTGGGT GAAATTTAAG GAATTTGTAA GGTTTGTGGT CATGGATCCC 
TTTGTTGACC TGGGAATCAC CATTTGCATC GTGCTTAACA CCCTTTTTAT GGCCATGGAG 
CACTACCCAA TGACTGAGCA GTTTGAGGGT GTTCTGAATG TAGGAAACTT GGTCTTTACT 
GGCATATTTG CAGCTGAAAT GTTCTTCAAG ATCATAGCCC TGGATCCATA TTACTACTTC 
CAAGTGGGGT GGAACATATT CGACAGCATT ATAGTCACTT TAAGTTTGGT GGAGTTGGGC 
CTTGCAAATG TGGAAGGTCT GTCCGTGCTC AGATCGTTCC GTCTGCTCAG GGTGTTCAAG 
CTAGCCAAAT CATGGCCAAC CCTTAACATG CTTATCAAAA TCATCGGTAA CTCGGTCGGA 
GCACTTGGAA ACCTCACCTT GGTGTTGGCC ATCATTGTCT TCATCTTTGC GGTGGTGGGG 
ATGCAGCTAT TTGGGAAGAG CTACAAGGAA TGTGTGTGCA AGATTGCCTC TGACTGCGAG 
CTACCACGGT GGCACATGCA CGATTTCTTC CACTCTTTTC TCATTGTCTT CCGTATCCTG 
TGCGGGGAGT GGATAGAGAC AATGTGGGAC TGCATGGAAG TGGCAGGGCA AGCAATGTGC 
CTGGTCGTGT TCATGATGGT CATGGTGATT GGTAACCTGG TGGTCCTCAA TCTCTTTTTG 
GCTTTGTTGC TGAGTTCCTT CAGCGCGGAT AACTTGTCGG CTTCGGACGA CGATGGGGAG 
ATGAACAACC TGCAGATCGC CATCGGAAGG ATCACCAGGG GCATTGACTT TGTGAAGAAA 
ACCTTCTTCT CCATCCTACA TTACAAGAGG AAGGCAAAAG CTGAGCAGGA GGAAGAGGAC 
CCTAACAAAG AAAACATTGC TCTGAACCAT GTAGAGACAG AACCAGAACC CAAGATAGAA 
CTAGAACCCA GGACAGAACC AGAACCCAAA AAAGAACCTC CACTGATTGA TGGCGTGACT 
AAAAAACGTT ATTACATGGA TGAGTTTGAC CACGCTAATT TTTTTAACAA CCCACACATG 
AGAATAGAAG TACCTATTGC CTCAGAAGAG TCCGACTTCG ACATTCCAGA CACGGATGAG 
TTTAGCACAC AGTCGGAGGT AGAGGAAGGG ATTTTACACC ACAAGAAGTA CGATGATGGC 
TCGTCAGTGT GTAGCACAGC AGATTATAAA CCTCCTGAAC CTGAAGAGGA AGAGGAGGCC 
TTTGAACACA AAGAAGACGA AGGCCCAGAA GAATGCTTTA CTGCTGACTG TGTACGAAGA 
TGCCCTTGTC TTTATGTCGA CATCACAACA GAAAAAGGGA AAATTTGGTG GAACATTCGG 
AAAACATGTT TCCAGATTGT GGAGCATAAC TGGTTTGAAA CCTTCATCAT CTTCATGATC 
TTACTCAGTA GTGGAGCACT GGCATTTGAG GATATCTACA TTGAACAGAG GCAAGTGATA 
AAGACTATCC TGGAGTACGC TGATAAAGTC TTCACATATA TTTTTGTGTT TGAAATGCTG 
CTAAAGTGGA CAGCGTATGG CTTCAAGATC TATTTCACGA ATGCCTGGTG CTGGCTGGAC 
TTCCTAATTG TTGACGTGTC TTTAATCAGC CTGACAGCCA ACTGGCTGGG CTACTCGGAA 
CTTGGTCCCA TCAAATCCCT TAGAACGCTG AGGGCCCTTA GGCCGTTACG AGCGTTATCA 
AGATTTGAAG GCATGAGGGT GGTTGTAAAC GCCCTTCTGG GAGCCATTCC TTCTATCATG 
AATGTCCTGC TTGTTTGTCT GATCTTCTGG CTCATCTTCA GCATCATGGG TGTTAACCTC 
TTTGCTGGGA AATATTATCG ATGCATCAAC ACGACCACGT CAGAATTATT TGAGATCGAT 
GTGGTCAACA ACCGCTCGGA CTGCCTCGCT TTAAACAACT CCGAGCAAGT GCGATGGGTT 
AATGTCAAAG TGAATTTTGA CAATGTGGGC CTTGGGTATC TCTCCCTTCT ACAAGTGGCA 
ACCTTCAAAG GCTGGATGGA TATCATGTAT GCCGCTGTAG ATTCCCGGAA TATTGAGGAT 
CAGCCACAAT ATGAAATAAA TCTTTATATG TATTTGTACT TTGTAATATT CATTATTTTT 
GGAGCTTTCT TCACTCTAAA CCTGTTTATC GGTGTCATTA TCGACAACTT CAATCAGCAA 
AAGAAAAAGT TTGGAGGTAA GGATATCTTT ATGACTGAGG AACAGAAGAA ATACTACAAT 
GCTATGAAAA AGCTGGGATC CAAAAAACCC CAAAAGCCAA TTCCAAGACC CACGAACAAA 
ATTCAAGGTT TGGTGTATGA CTTTGTCACA AAGCAGCTCT TTGACATTGT GATTATGATT 
TTAATTTGCC TCAACATGGT GACGATGATG ATTGAAACAG ATGACCAGAG TCAGGAAAAA 
ACTGATATTC TTTTCAAGAT CAATCTGGTG TTCATTGTCA TCTTCACAGC CGAATGTGTT 
CTCAAAATGT TTGCGCTGAG GTACTACTTT TTCACAATAG GATGGAACAT ATTTGACTTT 
GTGGTGGTCA TCCTATCTAT TGTTGGTATT GTTCTCTCGG ATCTCATAGC AGAGTACTTT 
GTTTCACCAA CACTTTTCAG AGTCATCAGA CTGGCGAGAA TCGGTAGAGT TCTTCGACTC 
ATAAGAGGAG CAAAGGGGAT TCGCACATTA CTTTTTGCCC TGATGATGTC TCTCCCCGCT 
CTCTTTAATA TCGGGCTTTT GCTTTTCTTA GTTATGTTCA TCTATTCAAT CTTTGGAATG 
TCCAATTTTG CCTATGTAAA GAAAGAATCT GGAATAGATG ACATATTTAA TTTTGAAACC 
TTTGGAAACA GCATAATATG CTTGTTCCAA ATCACCACGT CTGCTGGTTG GGATGGTCTT 
TTAAATCCAA TCCTAAATAG CGGCCCACCA GATTGTGACC CCCATCTGGA GAACTTTGGA 
ACAACAGTTA CTGGTGATTG TGGTAACCCA GCTATAGGTA TTGTGTTCTT CTGCAGCTAC 
ATCATAATAT CATTTTTAAT AGTTGTCAAT ATGTACATTG CCATTATCCT GGAGAACTTT 
AATGTTGCCA CAGAAGAGAG TGGTGAGCCA TTATGTGAGG ATGACTTTGA GATGTTTTAT 
GAAACTTGGG AGAAGTTTGA TCCAGATGCC ACTCAGTTTG TTGAATACAG TCGAATGTCA 
GACTTTGTAG ACACTTTGCA AGATCCTCTA CGGATTCCGA AACCTAACAA AATAAAACTA 
ATTACTTTGG ATCTCCCAAT GGTAACTGGA GATAAGATTC ACTGCTTGGA TATTCTGTTT 
GCACTAACTA AAGAGGTGTT GGGGGACTCA GGAGAAATGG ATGCATTAAA AGCCTCAATG 
GAAGAAAAGT TTATGATGGC AAATCCATCA AAAGTGTCTT ATGAACCAAT CACCACCACC 
CTTAAAAGAA AGCAGGAAGA AGTCTGTGTC ATCAAAATCC AGAGGGCCTT TAGGAGACAT 
CTATTGAGAC GTTCGGTGAA ACACGCTTCC TACATCTTCA GGCAAAGTAA TGATGAAGAG 
TCATCAGTGG AGGAACCTCC TGAAAAAGAA GGGTTGATAG CTGAAAGAAT TAATGCAGCC 
TACGGAAAAT ATGTAGAGGA AGGGCAAAAC GGCGGAACTC ATCGCCCAAG ATCAACAAAC 
AGAACTTCAC ACAGCAGTAT AGATTATGAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031750390.1
CDS	158..5650
Translation	MAGKVHPPGPDSLRPFTRESLAAIEKRIEEDIARKAKQIEVLDEKVPTPKNGLEAGKNLPLIYGDPPPELIGIPIEELDSFYSNQKTFIVLNKGKAIFRFTATKALYLLDPFNPIRRGAIKVLIHSLFSLFIMFTILTNCVFMTMSDPPPWSKNVEYTFTGIYTFESLIKILARGFCIDSFTFLRDPWNWLDFCVIVMAYTTEFVDLGNVSALRTFRVLRALKTITVIPGLKTIVGALIQSVKKLADVMILTLFCLAVFALIGLQLFMGNLRHKCVRWPPPDDYFMLYDNSTLNSTLYNDTMINSTLSNSTNVFDNDTIDWGAYLNDEANFYFLDGANDALLCGNSSDAGKCPESYTCMKTGRNPNYGYTSYDSFNWAFLSLFRLMTQDFWENLFQLTLRAAGKTYMVFFVVVIFLGSFYLINLILAVVAMAYAEQNEATLQEEIEKEKEFQELLLQLKKHQEEQARRKSSVASVGLLNAEKKNALSENTLSGDGTENMDCNGRTVPKVIIERAPSMEDTSSAQSDQQRNSKVYLEEPTLQKRAESTHSVLSEVALEELEEAHQKCPPWWYKFANTFLIWDCCDLWVKFKEFVRFVVMDPFVDLGITICIVLNTLFMAMEHYPMTEQFEGVLNVGNLVFTGIFAAEMFFKIIALDPYYYFQVGWNIFDSIIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKIASDCELPRWHMHDFFHSFLIVFRILCGEWIETMWDCMEVAGQAMCLVVFMMVMVIGNLVVLNLFLALLLSSFSADNLSASDDDGEMNNLQIAIGRITRGIDFVKKTFFSILHYKRKAKAEQEEEDPNKENIALNHVETEPEPKIELEPRTEPEPKKEPPLIDGVTKKRYYMDEFDHANFFNNPHMRIEVPIASEESDFDIPDTDEFSTQSEVEEGILHHKKYDDGSSVCSTADYKPPEPEEEEEAFEHKEDEGPEECFTADCVRRCPCLYVDITTEKGKIWWNIRKTCFQIVEHNWFETFIIFMILLSSGALAFEDIYIEQRQVIKTILEYADKVFTYIFVFEMLLKWTAYGFKIYFTNAWCWLDFLIVDVSLISLTANWLGYSELGPIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKYYRCINTTTSELFEIDVVNNRSDCLALNNSEQVRWVNVKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSRNIEDQPQYEINLYMYLYFVIFIIFGAFFTLNLFIGVIIDNFNQQKKKFGGKDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPTNKIQGLVYDFVTKQLFDIVIMILICLNMVTMMIETDDQSQEKTDILFKINLVFIVIFTAECVLKMFALRYYFFTIGWNIFDFVVVILSIVGIVLSDLIAEYFVSPTLFRVIRLARIGRVLRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMSNFAYVKKESGIDDIFNFETFGNSIICLFQITTSAGWDGLLNPILNSGPPDCDPHLENFGTTVTGDCGNPAIGIVFFCSYIIISFLIVVNMYIAIILENFNVATEESGEPLCEDDFEMFYETWEKFDPDATQFVEYSRMSDFVDTLQDPLRIPKPNKIKLITLDLPMVTGDKIHCLDILFALTKEVLGDSGEMDALKASMEEKFMMANPSKVSYEPITTTLKRKQEEVCVIKIQRAFRRHLLRRSVKHASYIFRQSNDEESSVEEPPEKEGLIAERINAAYGKYVEEGQNGGTHRPRSTNRTSHSSIDYD

Target ORF information:

RefSeq Version	XM_031894530.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sodium channel, voltage gated, type IV alpha subunit (scn4a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031894530.1

ORF Insert Sequence:

ATGGCTGGCA AGGTGCACCC TCCCGGTCCT GATAGTTTGC GTCCATTTAC CAGAGAGTCC 
TTGGCTGCGA TAGAGAAGCG CATTGAGGAG GACATCGCTC GGAAAGCCAA ACAGATTGAG 
GTGCTCGATG AGAAAGTACC GACACCCAAA AATGGACTGG AGGCAGGAAA GAACTTGCCT 
CTTATATATG GGGATCCCCC ACCCGAACTT ATTGGGATTC CCATAGAGGA ACTAGATTCT 
TTTTACAGCA ACCAAAAGAC ATTTATCGTC TTAAACAAAG GAAAAGCAAT ATTCCGATTC 
ACAGCCACCA AAGCCTTATA TCTACTGGAT CCATTTAATC CAATCAGAAG AGGCGCTATT 
AAAGTCTTAA TCCATTCATT GTTTAGCTTA TTTATTATGT TTACCATCTT AACAAATTGT 
GTTTTTATGA CCATGAGTGA CCCACCACCC TGGTCCAAGA ATGTAGAGTA CACGTTTACT 
GGAATTTACA CCTTTGAGTC CTTAATAAAA ATCCTGGCTC GGGGTTTTTG TATTGACAGT 
TTTACATTCC TCAGGGATCC GTGGAACTGG CTGGATTTCT GTGTCATTGT TATGGCTTAC 
ACCACAGAGT TTGTAGATTT GGGGAATGTG TCTGCCCTTA GGACATTCCG TGTACTCCGT 
GCTCTTAAAA CAATTACAGT TATTCCAGGC CTGAAGACTA TCGTTGGTGC CTTGATCCAA 
TCAGTGAAGA AGCTCGCAGA TGTGATGATC CTCACCCTCT TCTGCCTTGC TGTCTTTGCC 
CTCATTGGCC TTCAGCTCTT TATGGGAAAT CTGAGGCACA AATGTGTCCG ATGGCCACCC 
CCTGATGACT ATTTCATGCT TTATGACAAC AGTACCTTAA ACTCCACACT ATATAATGAT 
ACAATGATCA ACTCCACTCT CAGCAACAGC ACAAATGTGT TCGACAACGA CACCATTGAC 
TGGGGTGCCT ATCTCAACGA TGAAGCAAAT TTTTACTTCC TGGATGGAGC AAATGATGCA 
CTTCTGTGTG GGAACAGCAG TGATGCCGGG AAATGTCCAG AAAGTTACAC ATGTATGAAA 
ACTGGGAGAA ATCCGAATTA TGGGTACACC AGCTACGACT CATTCAACTG GGCTTTCCTT 
TCCCTTTTCC GATTAATGAC ACAGGACTTC TGGGAGAATC TTTTCCAGTT GACACTACGA 
GCAGCTGGTA AAACCTACAT GGTCTTCTTT GTGGTGGTAA TTTTTCTGGG GTCTTTCTAT 
CTGATCAACT TGATCCTTGC TGTGGTGGCC ATGGCATATG CAGAACAGAA TGAAGCCACT 
CTGCAAGAAG AAATAGAAAA GGAGAAAGAG TTCCAGGAGC TTTTGTTGCA GCTAAAGAAA 
CATCAAGAAG AGCAAGCAAG AAGGAAATCC AGCGTTGCCT CAGTTGGACT TCTGAACGCA 
GAAAAGAAAA ATGCTCTTTC AGAGAACACT CTTTCTGGGG ATGGCACAGA AAATATGGAC 
TGCAATGGGC GAACTGTGCC CAAAGTTATA ATTGAACGTG CACCCTCAAT GGAGGATACA 
AGCTCAGCAC AGTCAGACCA ACAGAGGAAT TCTAAAGTTT ATTTGGAGGA GCCAACTCTG 
CAGAAAAGAG CAGAGAGCAC CCACAGTGTC CTATCGGAAG TAGCTCTTGA AGAACTGGAA 
GAGGCTCATC AGAAATGCCC TCCTTGGTGG TACAAGTTTG CTAACACCTT TTTGATTTGG 
GACTGCTGTG ACCTGTGGGT GAAATTTAAG GAATTTGTAA GGTTTGTGGT CATGGATCCC 
TTTGTTGACC TGGGAATCAC CATTTGCATC GTGCTTAACA CCCTTTTTAT GGCCATGGAG 
CACTACCCAA TGACTGAGCA GTTTGAGGGT GTTCTGAATG TAGGAAACTT GGTCTTTACT 
GGCATATTTG CAGCTGAAAT GTTCTTCAAG ATCATAGCCC TGGATCCATA TTACTACTTC 
CAAGTGGGGT GGAACATATT CGACAGCATT ATAGTCACTT TAAGTTTGGT GGAGTTGGGC 
CTTGCAAATG TGGAAGGTCT GTCCGTGCTC AGATCGTTCC GTCTGCTCAG GGTGTTCAAG 
CTAGCCAAAT CATGGCCAAC CCTTAACATG CTTATCAAAA TCATCGGTAA CTCGGTCGGA 
GCACTTGGAA ACCTCACCTT GGTGTTGGCC ATCATTGTCT TCATCTTTGC GGTGGTGGGG 
ATGCAGCTAT TTGGGAAGAG CTACAAGGAA TGTGTGTGCA AGATTGCCTC TGACTGCGAG 
CTACCACGGT GGCACATGCA CGATTTCTTC CACTCTTTTC TCATTGTCTT CCGTATCCTG 
TGCGGGGAGT GGATAGAGAC AATGTGGGAC TGCATGGAAG TGGCAGGGCA AGCAATGTGC 
CTGGTCGTGT TCATGATGGT CATGGTGATT GGTAACCTGG TGGTCCTCAA TCTCTTTTTG 
GCTTTGTTGC TGAGTTCCTT CAGCGCGGAT AACTTGTCGG CTTCGGACGA CGATGGGGAG 
ATGAACAACC TGCAGATCGC CATCGGAAGG ATCACCAGGG GCATTGACTT TGTGAAGAAA 
ACCTTCTTCT CCATCCTACA TTACAAGAGG AAGGCAAAAG CTGAGCAGGA GGAAGAGGAC 
CCTAACAAAG AAAACATTGC TCTGAACCAT GTAGAGACAG AACCAGAACC CAAGATAGAA 
CTAGAACCCA GGACAGAACC AGAACCCAAA AAAGAACCTC CACTGATTGA TGGCGTGACT 
AAAAAACGTT ATTACATGGA TGAGTTTGAC CACGCTAATT TTTTTAACAA CCCACACATG 
AGAATAGAAG TACCTATTGC CTCAGAAGAG TCCGACTTCG ACATTCCAGA CACGGATGAG 
TTTAGCACAC AGTCGGAGGT AGAGGAAGGG ATTTTACACC ACAAGAAGTA CGATGATGGC 
TCGTCAGTGT GTAGCACAGC AGATTATAAA CCTCCTGAAC CTGAAGAGGA AGAGGAGGCC 
TTTGAACACA AAGAAGACGA AGGCCCAGAA GAATGCTTTA CTGCTGACTG TGTACGAAGA 
TGCCCTTGTC TTTATGTCGA CATCACAACA GAAAAAGGGA AAATTTGGTG GAACATTCGG 
AAAACATGTT TCCAGATTGT GGAGCATAAC TGGTTTGAAA CCTTCATCAT CTTCATGATC 
TTACTCAGTA GTGGAGCACT GGCATTTGAG GATATCTACA TTGAACAGAG GCAAGTGATA 
AAGACTATCC TGGAGTACGC TGATAAAGTC TTCACATATA TTTTTGTGTT TGAAATGCTG 
CTAAAGTGGA CAGCGTATGG CTTCAAGATC TATTTCACGA ATGCCTGGTG CTGGCTGGAC 
TTCCTAATTG TTGACGTGTC TTTAATCAGC CTGACAGCCA ACTGGCTGGG CTACTCGGAA 
CTTGGTCCCA TCAAATCCCT TAGAACGCTG AGGGCCCTTA GGCCGTTACG AGCGTTATCA 
AGATTTGAAG GCATGAGGGT GGTTGTAAAC GCCCTTCTGG GAGCCATTCC TTCTATCATG 
AATGTCCTGC TTGTTTGTCT GATCTTCTGG CTCATCTTCA GCATCATGGG TGTTAACCTC 
TTTGCTGGGA AATATTATCG ATGCATCAAC ACGACCACGT CAGAATTATT TGAGATCGAT 
GTGGTCAACA ACCGCTCGGA CTGCCTCGCT TTAAACAACT CCGAGCAAGT GCGATGGGTT 
AATGTCAAAG TGAATTTTGA CAATGTGGGC CTTGGGTATC TCTCCCTTCT ACAAGTGGCA 
ACCTTCAAAG GCTGGATGGA TATCATGTAT GCCGCTGTAG ATTCCCGGAA TATTGAGGAT 
CAGCCACAAT ATGAAATAAA TCTTTATATG TATTTGTACT TTGTAATATT CATTATTTTT 
GGAGCTTTCT TCACTCTAAA CCTGTTTATC GGTGTCATTA TCGACAACTT CAATCAGCAA 
AAGAAAAAGT TTGGAGGTAA GGATATCTTT ATGACTGAGG AACAGAAGAA ATACTACAAT 
GCTATGAAAA AGCTGGGATC CAAAAAACCC CAAAAGCCAA TTCCAAGACC CACGAACAAA 
ATTCAAGGTT TGGTGTATGA CTTTGTCACA AAGCAGCTCT TTGACATTGT GATTATGATT 
TTAATTTGCC TCAACATGGT GACGATGATG ATTGAAACAG ATGACCAGAG TCAGGAAAAA 
ACTGATATTC TTTTCAAGAT CAATCTGGTG TTCATTGTCA TCTTCACAGC CGAATGTGTT 
CTCAAAATGT TTGCGCTGAG GTACTACTTT TTCACAATAG GATGGAACAT ATTTGACTTT 
GTGGTGGTCA TCCTATCTAT TGTTGGTATT GTTCTCTCGG ATCTCATAGC AGAGTACTTT 
GTTTCACCAA CACTTTTCAG AGTCATCAGA CTGGCGAGAA TCGGTAGAGT TCTTCGACTC 
ATAAGAGGAG CAAAGGGGAT TCGCACATTA CTTTTTGCCC TGATGATGTC TCTCCCCGCT 
CTCTTTAATA TCGGGCTTTT GCTTTTCTTA GTTATGTTCA TCTATTCAAT CTTTGGAATG 
TCCAATTTTG CCTATGTAAA GAAAGAATCT GGAATAGATG ACATATTTAA TTTTGAAACC 
TTTGGAAACA GCATAATATG CTTGTTCCAA ATCACCACGT CTGCTGGTTG GGATGGTCTT 
TTAAATCCAA TCCTAAATAG CGGCCCACCA GATTGTGACC CCCATCTGGA GAACTTTGGA 
ACAACAGTTA CTGGTGATTG TGGTAACCCA GCTATAGGTA TTGTGTTCTT CTGCAGCTAC 
ATCATAATAT CATTTTTAAT AGTTGTCAAT ATGTACATTG CCATTATCCT GGAGAACTTT 
AATGTTGCCA CAGAAGAGAG TGGTGAGCCA TTATGTGAGG ATGACTTTGA GATGTTTTAT 
GAAACTTGGG AGAAGTTTGA TCCAGATGCC ACTCAGTTTG TTGAATACAG TCGAATGTCA 
GACTTTGTAG ACACTTTGCA AGATCCTCTA CGGATTCCGA AACCTAACAA AATAAAACTA 
ATTACTTTGG ATCTCCCAAT GGTAACTGGA GATAAGATTC ACTGCTTGGA TATTCTGTTT 
GCACTAACTA AAGAGGTGTT GGGGGACTCA GGAGAAATGG ATGCATTAAA AGCCTCAATG 
GAAGAAAAGT TTATGATGGC AAATCCATCA AAAGTGTCTT ATGAACCAAT CACCACCACC 
CTTAAAAGAA AGCAGGAAGA AGTCTGTGTC ATCAAAATCC AGAGGGCCTT TAGGAGACAT 
CTATTGAGAC GTTCGGTGAA ACACGCTTCC TACATCTTCA GGCAAAGTAA TGATGAAGAG 
TCATCAGTGG AGGAACCTCC TGAAAAAGAA GGGTTGATAG CTGAAAGAAT TAATGCAGCC 
TACGGAAAAT ATGTAGAGGA AGGGCAAAAC GGCGGAACTC ATCGCCCAAG ATCAACAAAC 
AGAACTTCAC ACAGCAGTAT AGATTATGAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scn4a ( XM_031894529.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031750389.1 Xenopus tropicalis sodium channel, voltage gated, type IV alpha subunit (scn4a), transcript variant X6, mRNA.

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CloneID	OXa50620
Clone ID Related Accession (Same CDS sequence)	XM_031894530.1 , XM_031894529.1
Accession Version	XM_031894529.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sodium channel protein type 4 subunit alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGCA AGGTGCACCC TCCCGGTCCT GATAGTTTGC GTCCATTTAC CAGAGAGTCC 
TTGGCTGCGA TAGAGAAGCG CATTGAGGAG GACATCGCTC GGAAAGCCAA ACAGATTGAG 
GTGCTCGATG AGAAAGTACC GACACCCAAA AATGGACTGG AGGCAGGAAA GAACTTGCCT 
CTTATATATG GGGATCCCCC ACCCGAACTT ATTGGGATTC CCATAGAGGA ACTAGATTCT 
TTTTACAGCA ACCAAAAGAC ATTTATCGTC TTAAACAAAG GAAAAGCAAT ATTCCGATTC 
ACAGCCACCA AAGCCTTATA TCTACTGGAT CCATTTAATC CAATCAGAAG AGGCGCTATT 
AAAGTCTTAA TCCATTCATT GTTTAGCTTA TTTATTATGT TTACCATCTT AACAAATTGT 
GTTTTTATGA CCATGAGTGA CCCACCACCC TGGTCCAAGA ATGTAGAGTA CACGTTTACT 
GGAATTTACA CCTTTGAGTC CTTAATAAAA ATCCTGGCTC GGGGTTTTTG TATTGACAGT 
TTTACATTCC TCAGGGATCC GTGGAACTGG CTGGATTTCT GTGTCATTGT TATGGCTTAC 
ACCACAGAGT TTGTAGATTT GGGGAATGTG TCTGCCCTTA GGACATTCCG TGTACTCCGT 
GCTCTTAAAA CAATTACAGT TATTCCAGGC CTGAAGACTA TCGTTGGTGC CTTGATCCAA 
TCAGTGAAGA AGCTCGCAGA TGTGATGATC CTCACCCTCT TCTGCCTTGC TGTCTTTGCC 
CTCATTGGCC TTCAGCTCTT TATGGGAAAT CTGAGGCACA AATGTGTCCG ATGGCCACCC 
CCTGATGACT ATTTCATGCT TTATGACAAC AGTACCTTAA ACTCCACACT ATATAATGAT 
ACAATGATCA ACTCCACTCT CAGCAACAGC ACAAATGTGT TCGACAACGA CACCATTGAC 
TGGGGTGCCT ATCTCAACGA TGAAGCAAAT TTTTACTTCC TGGATGGAGC AAATGATGCA 
CTTCTGTGTG GGAACAGCAG TGATGCCGGG AAATGTCCAG AAAGTTACAC ATGTATGAAA 
ACTGGGAGAA ATCCGAATTA TGGGTACACC AGCTACGACT CATTCAACTG GGCTTTCCTT 
TCCCTTTTCC GATTAATGAC ACAGGACTTC TGGGAGAATC TTTTCCAGTT GACACTACGA 
GCAGCTGGTA AAACCTACAT GGTCTTCTTT GTGGTGGTAA TTTTTCTGGG GTCTTTCTAT 
CTGATCAACT TGATCCTTGC TGTGGTGGCC ATGGCATATG CAGAACAGAA TGAAGCCACT 
CTGCAAGAAG AAATAGAAAA GGAGAAAGAG TTCCAGGAGC TTTTGTTGCA GCTAAAGAAA 
CATCAAGAAG AGCAAGCAAG AAGGAAATCC AGCGTTGCCT CAGTTGGACT TCTGAACGCA 
GAAAAGAAAA ATGCTCTTTC AGAGAACACT CTTTCTGGGG ATGGCACAGA AAATATGGAC 
TGCAATGGGC GAACTGTGCC CAAAGTTATA ATTGAACGTG CACCCTCAAT GGAGGATACA 
AGCTCAGCAC AGTCAGACCA ACAGAGGAAT TCTAAAGTTT ATTTGGAGGA GCCAACTCTG 
CAGAAAAGAG CAGAGAGCAC CCACAGTGTC CTATCGGAAG TAGCTCTTGA AGAACTGGAA 
GAGGCTCATC AGAAATGCCC TCCTTGGTGG TACAAGTTTG CTAACACCTT TTTGATTTGG 
GACTGCTGTG ACCTGTGGGT GAAATTTAAG GAATTTGTAA GGTTTGTGGT CATGGATCCC 
TTTGTTGACC TGGGAATCAC CATTTGCATC GTGCTTAACA CCCTTTTTAT GGCCATGGAG 
CACTACCCAA TGACTGAGCA GTTTGAGGGT GTTCTGAATG TAGGAAACTT GGTCTTTACT 
GGCATATTTG CAGCTGAAAT GTTCTTCAAG ATCATAGCCC TGGATCCATA TTACTACTTC 
CAAGTGGGGT GGAACATATT CGACAGCATT ATAGTCACTT TAAGTTTGGT GGAGTTGGGC 
CTTGCAAATG TGGAAGGTCT GTCCGTGCTC AGATCGTTCC GTCTGCTCAG GGTGTTCAAG 
CTAGCCAAAT CATGGCCAAC CCTTAACATG CTTATCAAAA TCATCGGTAA CTCGGTCGGA 
GCACTTGGAA ACCTCACCTT GGTGTTGGCC ATCATTGTCT TCATCTTTGC GGTGGTGGGG 
ATGCAGCTAT TTGGGAAGAG CTACAAGGAA TGTGTGTGCA AGATTGCCTC TGACTGCGAG 
CTACCACGGT GGCACATGCA CGATTTCTTC CACTCTTTTC TCATTGTCTT CCGTATCCTG 
TGCGGGGAGT GGATAGAGAC AATGTGGGAC TGCATGGAAG TGGCAGGGCA AGCAATGTGC 
CTGGTCGTGT TCATGATGGT CATGGTGATT GGTAACCTGG TGGTCCTCAA TCTCTTTTTG 
GCTTTGTTGC TGAGTTCCTT CAGCGCGGAT AACTTGTCGG CTTCGGACGA CGATGGGGAG 
ATGAACAACC TGCAGATCGC CATCGGAAGG ATCACCAGGG GCATTGACTT TGTGAAGAAA 
ACCTTCTTCT CCATCCTACA TTACAAGAGG AAGGCAAAAG CTGAGCAGGA GGAAGAGGAC 
CCTAACAAAG AAAACATTGC TCTGAACCAT GTAGAGACAG AACCAGAACC CAAGATAGAA 
CTAGAACCCA GGACAGAACC AGAACCCAAA AAAGAACCTC CACTGATTGA TGGCGTGACT 
AAAAAACGTT ATTACATGGA TGAGTTTGAC CACGCTAATT TTTTTAACAA CCCACACATG 
AGAATAGAAG TACCTATTGC CTCAGAAGAG TCCGACTTCG ACATTCCAGA CACGGATGAG 
TTTAGCACAC AGTCGGAGGT AGAGGAAGGG ATTTTACACC ACAAGAAGTA CGATGATGGC 
TCGTCAGTGT GTAGCACAGC AGATTATAAA CCTCCTGAAC CTGAAGAGGA AGAGGAGGCC 
TTTGAACACA AAGAAGACGA AGGCCCAGAA GAATGCTTTA CTGCTGACTG TGTACGAAGA 
TGCCCTTGTC TTTATGTCGA CATCACAACA GAAAAAGGGA AAATTTGGTG GAACATTCGG 
AAAACATGTT TCCAGATTGT GGAGCATAAC TGGTTTGAAA CCTTCATCAT CTTCATGATC 
TTACTCAGTA GTGGAGCACT GGCATTTGAG GATATCTACA TTGAACAGAG GCAAGTGATA 
AAGACTATCC TGGAGTACGC TGATAAAGTC TTCACATATA TTTTTGTGTT TGAAATGCTG 
CTAAAGTGGA CAGCGTATGG CTTCAAGATC TATTTCACGA ATGCCTGGTG CTGGCTGGAC 
TTCCTAATTG TTGACGTGTC TTTAATCAGC CTGACAGCCA ACTGGCTGGG CTACTCGGAA 
CTTGGTCCCA TCAAATCCCT TAGAACGCTG AGGGCCCTTA GGCCGTTACG AGCGTTATCA 
AGATTTGAAG GCATGAGGGT GGTTGTAAAC GCCCTTCTGG GAGCCATTCC TTCTATCATG 
AATGTCCTGC TTGTTTGTCT GATCTTCTGG CTCATCTTCA GCATCATGGG TGTTAACCTC 
TTTGCTGGGA AATATTATCG ATGCATCAAC ACGACCACGT CAGAATTATT TGAGATCGAT 
GTGGTCAACA ACCGCTCGGA CTGCCTCGCT TTAAACAACT CCGAGCAAGT GCGATGGGTT 
AATGTCAAAG TGAATTTTGA CAATGTGGGC CTTGGGTATC TCTCCCTTCT ACAAGTGGCA 
ACCTTCAAAG GCTGGATGGA TATCATGTAT GCCGCTGTAG ATTCCCGGAA TATTGAGGAT 
CAGCCACAAT ATGAAATAAA TCTTTATATG TATTTGTACT TTGTAATATT CATTATTTTT 
GGAGCTTTCT TCACTCTAAA CCTGTTTATC GGTGTCATTA TCGACAACTT CAATCAGCAA 
AAGAAAAAGT TTGGAGGTAA GGATATCTTT ATGACTGAGG AACAGAAGAA ATACTACAAT 
GCTATGAAAA AGCTGGGATC CAAAAAACCC CAAAAGCCAA TTCCAAGACC CACGAACAAA 
ATTCAAGGTT TGGTGTATGA CTTTGTCACA AAGCAGCTCT TTGACATTGT GATTATGATT 
TTAATTTGCC TCAACATGGT GACGATGATG ATTGAAACAG ATGACCAGAG TCAGGAAAAA 
ACTGATATTC TTTTCAAGAT CAATCTGGTG TTCATTGTCA TCTTCACAGC CGAATGTGTT 
CTCAAAATGT TTGCGCTGAG GTACTACTTT TTCACAATAG GATGGAACAT ATTTGACTTT 
GTGGTGGTCA TCCTATCTAT TGTTGGTATT GTTCTCTCGG ATCTCATAGC AGAGTACTTT 
GTTTCACCAA CACTTTTCAG AGTCATCAGA CTGGCGAGAA TCGGTAGAGT TCTTCGACTC 
ATAAGAGGAG CAAAGGGGAT TCGCACATTA CTTTTTGCCC TGATGATGTC TCTCCCCGCT 
CTCTTTAATA TCGGGCTTTT GCTTTTCTTA GTTATGTTCA TCTATTCAAT CTTTGGAATG 
TCCAATTTTG CCTATGTAAA GAAAGAATCT GGAATAGATG ACATATTTAA TTTTGAAACC 
TTTGGAAACA GCATAATATG CTTGTTCCAA ATCACCACGT CTGCTGGTTG GGATGGTCTT 
TTAAATCCAA TCCTAAATAG CGGCCCACCA GATTGTGACC CCCATCTGGA GAACTTTGGA 
ACAACAGTTA CTGGTGATTG TGGTAACCCA GCTATAGGTA TTGTGTTCTT CTGCAGCTAC 
ATCATAATAT CATTTTTAAT AGTTGTCAAT ATGTACATTG CCATTATCCT GGAGAACTTT 
AATGTTGCCA CAGAAGAGAG TGGTGAGCCA TTATGTGAGG ATGACTTTGA GATGTTTTAT 
GAAACTTGGG AGAAGTTTGA TCCAGATGCC ACTCAGTTTG TTGAATACAG TCGAATGTCA 
GACTTTGTAG ACACTTTGCA AGATCCTCTA CGGATTCCGA AACCTAACAA AATAAAACTA 
ATTACTTTGG ATCTCCCAAT GGTAACTGGA GATAAGATTC ACTGCTTGGA TATTCTGTTT 
GCACTAACTA AAGAGGTGTT GGGGGACTCA GGAGAAATGG ATGCATTAAA AGCCTCAATG 
GAAGAAAAGT TTATGATGGC AAATCCATCA AAAGTGTCTT ATGAACCAAT CACCACCACC 
CTTAAAAGAA AGCAGGAAGA AGTCTGTGTC ATCAAAATCC AGAGGGCCTT TAGGAGACAT 
CTATTGAGAC GTTCGGTGAA ACACGCTTCC TACATCTTCA GGCAAAGTAA TGATGAAGAG 
TCATCAGTGG AGGAACCTCC TGAAAAAGAA GGGTTGATAG CTGAAAGAAT TAATGCAGCC 
TACGGAAAAT ATGTAGAGGA AGGGCAAAAC GGCGGAACTC ATCGCCCAAG ATCAACAAAC 
AGAACTTCAC ACAGCAGTAT AGATTATGAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031750389.1
CDS	158..5650
Translation	MAGKVHPPGPDSLRPFTRESLAAIEKRIEEDIARKAKQIEVLDEKVPTPKNGLEAGKNLPLIYGDPPPELIGIPIEELDSFYSNQKTFIVLNKGKAIFRFTATKALYLLDPFNPIRRGAIKVLIHSLFSLFIMFTILTNCVFMTMSDPPPWSKNVEYTFTGIYTFESLIKILARGFCIDSFTFLRDPWNWLDFCVIVMAYTTEFVDLGNVSALRTFRVLRALKTITVIPGLKTIVGALIQSVKKLADVMILTLFCLAVFALIGLQLFMGNLRHKCVRWPPPDDYFMLYDNSTLNSTLYNDTMINSTLSNSTNVFDNDTIDWGAYLNDEANFYFLDGANDALLCGNSSDAGKCPESYTCMKTGRNPNYGYTSYDSFNWAFLSLFRLMTQDFWENLFQLTLRAAGKTYMVFFVVVIFLGSFYLINLILAVVAMAYAEQNEATLQEEIEKEKEFQELLLQLKKHQEEQARRKSSVASVGLLNAEKKNALSENTLSGDGTENMDCNGRTVPKVIIERAPSMEDTSSAQSDQQRNSKVYLEEPTLQKRAESTHSVLSEVALEELEEAHQKCPPWWYKFANTFLIWDCCDLWVKFKEFVRFVVMDPFVDLGITICIVLNTLFMAMEHYPMTEQFEGVLNVGNLVFTGIFAAEMFFKIIALDPYYYFQVGWNIFDSIIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKIASDCELPRWHMHDFFHSFLIVFRILCGEWIETMWDCMEVAGQAMCLVVFMMVMVIGNLVVLNLFLALLLSSFSADNLSASDDDGEMNNLQIAIGRITRGIDFVKKTFFSILHYKRKAKAEQEEEDPNKENIALNHVETEPEPKIELEPRTEPEPKKEPPLIDGVTKKRYYMDEFDHANFFNNPHMRIEVPIASEESDFDIPDTDEFSTQSEVEEGILHHKKYDDGSSVCSTADYKPPEPEEEEEAFEHKEDEGPEECFTADCVRRCPCLYVDITTEKGKIWWNIRKTCFQIVEHNWFETFIIFMILLSSGALAFEDIYIEQRQVIKTILEYADKVFTYIFVFEMLLKWTAYGFKIYFTNAWCWLDFLIVDVSLISLTANWLGYSELGPIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKYYRCINTTTSELFEIDVVNNRSDCLALNNSEQVRWVNVKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSRNIEDQPQYEINLYMYLYFVIFIIFGAFFTLNLFIGVIIDNFNQQKKKFGGKDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPTNKIQGLVYDFVTKQLFDIVIMILICLNMVTMMIETDDQSQEKTDILFKINLVFIVIFTAECVLKMFALRYYFFTIGWNIFDFVVVILSIVGIVLSDLIAEYFVSPTLFRVIRLARIGRVLRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMSNFAYVKKESGIDDIFNFETFGNSIICLFQITTSAGWDGLLNPILNSGPPDCDPHLENFGTTVTGDCGNPAIGIVFFCSYIIISFLIVVNMYIAIILENFNVATEESGEPLCEDDFEMFYETWEKFDPDATQFVEYSRMSDFVDTLQDPLRIPKPNKIKLITLDLPMVTGDKIHCLDILFALTKEVLGDSGEMDALKASMEEKFMMANPSKVSYEPITTTLKRKQEEVCVIKIQRAFRRHLLRRSVKHASYIFRQSNDEESSVEEPPEKEGLIAERINAAYGKYVEEGQNGGTHRPRSTNRTSHSSIDYD

Target ORF information:

RefSeq Version	XM_031894529.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sodium channel, voltage gated, type IV alpha subunit (scn4a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031894529.1

ORF Insert Sequence:

ATGGCTGGCA AGGTGCACCC TCCCGGTCCT GATAGTTTGC GTCCATTTAC CAGAGAGTCC 
TTGGCTGCGA TAGAGAAGCG CATTGAGGAG GACATCGCTC GGAAAGCCAA ACAGATTGAG 
GTGCTCGATG AGAAAGTACC GACACCCAAA AATGGACTGG AGGCAGGAAA GAACTTGCCT 
CTTATATATG GGGATCCCCC ACCCGAACTT ATTGGGATTC CCATAGAGGA ACTAGATTCT 
TTTTACAGCA ACCAAAAGAC ATTTATCGTC TTAAACAAAG GAAAAGCAAT ATTCCGATTC 
ACAGCCACCA AAGCCTTATA TCTACTGGAT CCATTTAATC CAATCAGAAG AGGCGCTATT 
AAAGTCTTAA TCCATTCATT GTTTAGCTTA TTTATTATGT TTACCATCTT AACAAATTGT 
GTTTTTATGA CCATGAGTGA CCCACCACCC TGGTCCAAGA ATGTAGAGTA CACGTTTACT 
GGAATTTACA CCTTTGAGTC CTTAATAAAA ATCCTGGCTC GGGGTTTTTG TATTGACAGT 
TTTACATTCC TCAGGGATCC GTGGAACTGG CTGGATTTCT GTGTCATTGT TATGGCTTAC 
ACCACAGAGT TTGTAGATTT GGGGAATGTG TCTGCCCTTA GGACATTCCG TGTACTCCGT 
GCTCTTAAAA CAATTACAGT TATTCCAGGC CTGAAGACTA TCGTTGGTGC CTTGATCCAA 
TCAGTGAAGA AGCTCGCAGA TGTGATGATC CTCACCCTCT TCTGCCTTGC TGTCTTTGCC 
CTCATTGGCC TTCAGCTCTT TATGGGAAAT CTGAGGCACA AATGTGTCCG ATGGCCACCC 
CCTGATGACT ATTTCATGCT TTATGACAAC AGTACCTTAA ACTCCACACT ATATAATGAT 
ACAATGATCA ACTCCACTCT CAGCAACAGC ACAAATGTGT TCGACAACGA CACCATTGAC 
TGGGGTGCCT ATCTCAACGA TGAAGCAAAT TTTTACTTCC TGGATGGAGC AAATGATGCA 
CTTCTGTGTG GGAACAGCAG TGATGCCGGG AAATGTCCAG AAAGTTACAC ATGTATGAAA 
ACTGGGAGAA ATCCGAATTA TGGGTACACC AGCTACGACT CATTCAACTG GGCTTTCCTT 
TCCCTTTTCC GATTAATGAC ACAGGACTTC TGGGAGAATC TTTTCCAGTT GACACTACGA 
GCAGCTGGTA AAACCTACAT GGTCTTCTTT GTGGTGGTAA TTTTTCTGGG GTCTTTCTAT 
CTGATCAACT TGATCCTTGC TGTGGTGGCC ATGGCATATG CAGAACAGAA TGAAGCCACT 
CTGCAAGAAG AAATAGAAAA GGAGAAAGAG TTCCAGGAGC TTTTGTTGCA GCTAAAGAAA 
CATCAAGAAG AGCAAGCAAG AAGGAAATCC AGCGTTGCCT CAGTTGGACT TCTGAACGCA 
GAAAAGAAAA ATGCTCTTTC AGAGAACACT CTTTCTGGGG ATGGCACAGA AAATATGGAC 
TGCAATGGGC GAACTGTGCC CAAAGTTATA ATTGAACGTG CACCCTCAAT GGAGGATACA 
AGCTCAGCAC AGTCAGACCA ACAGAGGAAT TCTAAAGTTT ATTTGGAGGA GCCAACTCTG 
CAGAAAAGAG CAGAGAGCAC CCACAGTGTC CTATCGGAAG TAGCTCTTGA AGAACTGGAA 
GAGGCTCATC AGAAATGCCC TCCTTGGTGG TACAAGTTTG CTAACACCTT TTTGATTTGG 
GACTGCTGTG ACCTGTGGGT GAAATTTAAG GAATTTGTAA GGTTTGTGGT CATGGATCCC 
TTTGTTGACC TGGGAATCAC CATTTGCATC GTGCTTAACA CCCTTTTTAT GGCCATGGAG 
CACTACCCAA TGACTGAGCA GTTTGAGGGT GTTCTGAATG TAGGAAACTT GGTCTTTACT 
GGCATATTTG CAGCTGAAAT GTTCTTCAAG ATCATAGCCC TGGATCCATA TTACTACTTC 
CAAGTGGGGT GGAACATATT CGACAGCATT ATAGTCACTT TAAGTTTGGT GGAGTTGGGC 
CTTGCAAATG TGGAAGGTCT GTCCGTGCTC AGATCGTTCC GTCTGCTCAG GGTGTTCAAG 
CTAGCCAAAT CATGGCCAAC CCTTAACATG CTTATCAAAA TCATCGGTAA CTCGGTCGGA 
GCACTTGGAA ACCTCACCTT GGTGTTGGCC ATCATTGTCT TCATCTTTGC GGTGGTGGGG 
ATGCAGCTAT TTGGGAAGAG CTACAAGGAA TGTGTGTGCA AGATTGCCTC TGACTGCGAG 
CTACCACGGT GGCACATGCA CGATTTCTTC CACTCTTTTC TCATTGTCTT CCGTATCCTG 
TGCGGGGAGT GGATAGAGAC AATGTGGGAC TGCATGGAAG TGGCAGGGCA AGCAATGTGC 
CTGGTCGTGT TCATGATGGT CATGGTGATT GGTAACCTGG TGGTCCTCAA TCTCTTTTTG 
GCTTTGTTGC TGAGTTCCTT CAGCGCGGAT AACTTGTCGG CTTCGGACGA CGATGGGGAG 
ATGAACAACC TGCAGATCGC CATCGGAAGG ATCACCAGGG GCATTGACTT TGTGAAGAAA 
ACCTTCTTCT CCATCCTACA TTACAAGAGG AAGGCAAAAG CTGAGCAGGA GGAAGAGGAC 
CCTAACAAAG AAAACATTGC TCTGAACCAT GTAGAGACAG AACCAGAACC CAAGATAGAA 
CTAGAACCCA GGACAGAACC AGAACCCAAA AAAGAACCTC CACTGATTGA TGGCGTGACT 
AAAAAACGTT ATTACATGGA TGAGTTTGAC CACGCTAATT TTTTTAACAA CCCACACATG 
AGAATAGAAG TACCTATTGC CTCAGAAGAG TCCGACTTCG ACATTCCAGA CACGGATGAG 
TTTAGCACAC AGTCGGAGGT AGAGGAAGGG ATTTTACACC ACAAGAAGTA CGATGATGGC 
TCGTCAGTGT GTAGCACAGC AGATTATAAA CCTCCTGAAC CTGAAGAGGA AGAGGAGGCC 
TTTGAACACA AAGAAGACGA AGGCCCAGAA GAATGCTTTA CTGCTGACTG TGTACGAAGA 
TGCCCTTGTC TTTATGTCGA CATCACAACA GAAAAAGGGA AAATTTGGTG GAACATTCGG 
AAAACATGTT TCCAGATTGT GGAGCATAAC TGGTTTGAAA CCTTCATCAT CTTCATGATC 
TTACTCAGTA GTGGAGCACT GGCATTTGAG GATATCTACA TTGAACAGAG GCAAGTGATA 
AAGACTATCC TGGAGTACGC TGATAAAGTC TTCACATATA TTTTTGTGTT TGAAATGCTG 
CTAAAGTGGA CAGCGTATGG CTTCAAGATC TATTTCACGA ATGCCTGGTG CTGGCTGGAC 
TTCCTAATTG TTGACGTGTC TTTAATCAGC CTGACAGCCA ACTGGCTGGG CTACTCGGAA 
CTTGGTCCCA TCAAATCCCT TAGAACGCTG AGGGCCCTTA GGCCGTTACG AGCGTTATCA 
AGATTTGAAG GCATGAGGGT GGTTGTAAAC GCCCTTCTGG GAGCCATTCC TTCTATCATG 
AATGTCCTGC TTGTTTGTCT GATCTTCTGG CTCATCTTCA GCATCATGGG TGTTAACCTC 
TTTGCTGGGA AATATTATCG ATGCATCAAC ACGACCACGT CAGAATTATT TGAGATCGAT 
GTGGTCAACA ACCGCTCGGA CTGCCTCGCT TTAAACAACT CCGAGCAAGT GCGATGGGTT 
AATGTCAAAG TGAATTTTGA CAATGTGGGC CTTGGGTATC TCTCCCTTCT ACAAGTGGCA 
ACCTTCAAAG GCTGGATGGA TATCATGTAT GCCGCTGTAG ATTCCCGGAA TATTGAGGAT 
CAGCCACAAT ATGAAATAAA TCTTTATATG TATTTGTACT TTGTAATATT CATTATTTTT 
GGAGCTTTCT TCACTCTAAA CCTGTTTATC GGTGTCATTA TCGACAACTT CAATCAGCAA 
AAGAAAAAGT TTGGAGGTAA GGATATCTTT ATGACTGAGG AACAGAAGAA ATACTACAAT 
GCTATGAAAA AGCTGGGATC CAAAAAACCC CAAAAGCCAA TTCCAAGACC CACGAACAAA 
ATTCAAGGTT TGGTGTATGA CTTTGTCACA AAGCAGCTCT TTGACATTGT GATTATGATT 
TTAATTTGCC TCAACATGGT GACGATGATG ATTGAAACAG ATGACCAGAG TCAGGAAAAA 
ACTGATATTC TTTTCAAGAT CAATCTGGTG TTCATTGTCA TCTTCACAGC CGAATGTGTT 
CTCAAAATGT TTGCGCTGAG GTACTACTTT TTCACAATAG GATGGAACAT ATTTGACTTT 
GTGGTGGTCA TCCTATCTAT TGTTGGTATT GTTCTCTCGG ATCTCATAGC AGAGTACTTT 
GTTTCACCAA CACTTTTCAG AGTCATCAGA CTGGCGAGAA TCGGTAGAGT TCTTCGACTC 
ATAAGAGGAG CAAAGGGGAT TCGCACATTA CTTTTTGCCC TGATGATGTC TCTCCCCGCT 
CTCTTTAATA TCGGGCTTTT GCTTTTCTTA GTTATGTTCA TCTATTCAAT CTTTGGAATG 
TCCAATTTTG CCTATGTAAA GAAAGAATCT GGAATAGATG ACATATTTAA TTTTGAAACC 
TTTGGAAACA GCATAATATG CTTGTTCCAA ATCACCACGT CTGCTGGTTG GGATGGTCTT 
TTAAATCCAA TCCTAAATAG CGGCCCACCA GATTGTGACC CCCATCTGGA GAACTTTGGA 
ACAACAGTTA CTGGTGATTG TGGTAACCCA GCTATAGGTA TTGTGTTCTT CTGCAGCTAC 
ATCATAATAT CATTTTTAAT AGTTGTCAAT ATGTACATTG CCATTATCCT GGAGAACTTT 
AATGTTGCCA CAGAAGAGAG TGGTGAGCCA TTATGTGAGG ATGACTTTGA GATGTTTTAT 
GAAACTTGGG AGAAGTTTGA TCCAGATGCC ACTCAGTTTG TTGAATACAG TCGAATGTCA 
GACTTTGTAG ACACTTTGCA AGATCCTCTA CGGATTCCGA AACCTAACAA AATAAAACTA 
ATTACTTTGG ATCTCCCAAT GGTAACTGGA GATAAGATTC ACTGCTTGGA TATTCTGTTT 
GCACTAACTA AAGAGGTGTT GGGGGACTCA GGAGAAATGG ATGCATTAAA AGCCTCAATG 
GAAGAAAAGT TTATGATGGC AAATCCATCA AAAGTGTCTT ATGAACCAAT CACCACCACC 
CTTAAAAGAA AGCAGGAAGA AGTCTGTGTC ATCAAAATCC AGAGGGCCTT TAGGAGACAT 
CTATTGAGAC GTTCGGTGAA ACACGCTTCC TACATCTTCA GGCAAAGTAA TGATGAAGAG 
TCATCAGTGG AGGAACCTCC TGAAAAAGAA GGGTTGATAG CTGAAAGAAT TAATGCAGCC 
TACGGAAAAT ATGTAGAGGA AGGGCAAAAC GGCGGAACTC ATCGCCCAAG ATCAACAAAC 
AGAACTTCAC ACAGCAGTAT AGATTATGAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scn4a ( XM_018089322.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017944811.1 Xenopus tropicalis sodium channel, voltage gated, type IV alpha subunit (scn4a), mRNA.

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CloneID	OXa35367
Clone ID Related Accession (Same CDS sequence)	XM_018089322.1
Accession Version	XM_018089322.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sodium channel protein type 4 subunit alpha
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGCA AGGTGCACCC TCCCGGTCCT GATAGTTTGC GTCCATTTAC CAGAGAGTCC 
TTGGCTGCGA TAGAGAAGCG CATTGAGGAG GACATCGCTC GGAAAGCCAA ACAGATTGAG 
GTGCTCGATG AGAAAGTACC GACACCCAAA AATGGACTGG AGGCAGGAAA GAACTTGCCT 
CTTATATATG GGGATCCCCC ACCCGAACTT ATTGGGATTC CCATAGAGGA ACTAGATTCT 
TTTTACAGCA ACCAAAAGAC ATTTATCGTC TTAAACAAAG GAAAAGCAAT ATTCCGATTC 
ACAGCCACCA AAGCCTTATA TCTACTGGAT CCATTTAATC CAATCAGAAG AGGCGCTATT 
AAAGTCTTAA TCCATTCATT GTTTAGCTTA TTTATTATGT TTACCATCTT AACAAATTGT 
GTTTTTATGA CCATGAGTGA CCCACCACCC TGGTCCAAGA ATGTAGAGTA CACGTTTACT 
GGAATTTACA CCTTTGAGTC CTTAATAAAA ATCCTGGCTC GGGGTTTTTG TATTGACAGT 
TTTACATTCC TCAGGGATCC GTGGAACTGG CTGGATTTCT GTGTCATTGT TATGGCTTAC 
ACCACAGAGT TTGTAGATTT GGGGAATGTG TCTGCCCTTA GGACATTCCG TGTACTCCGT 
GCTCTTAAAA CAATTACAGT TATTCCAGGC CTGAAGACTA TCGTTGGTGC CTTGATCCAA 
TCAGTGAAGA AGCTCGCAGA TGTGATGATC CTCACCCTCT TCTGCCTTGC TGTCTTTGCC 
CTCATTGGCC TTCAGCTCTT TATGGGAAAT CTGAGGCACA AATGTGTCCG ATGGCCACCC 
CCTGATGACT ATTTCATGCT TTATGACAAC AGTACCTTAA ACTCCACACT ATATAATGAT 
ACAATGATCA ACTCCACTCT CAGCAACAGC ACAAATGTGT TCGACAACGA CACCATTGAC 
TGGGGTGCCT ATCTCAACGA TGAAGCAAAT TTTTACTTCC TGGATGGAGC AAATGATGCA 
CTTCTGTGTG GGAACAGCAG TGATGCCGGG AAATGTCCAG AAAGTTACAC ATGTATGAAA 
ACTGGGAGAA ATCCTAATTA TGGGTACACC AGCTACGACT CATTCAACTG GGCTTTCCTT 
TCCCTTTTCC GATTAATGAC ACAGGACTTC TGGGAGAATC TTTTCCAGTT GACACTACGA 
GCAGCTGGTA AAACCTACAT GGTCTTCTTT GTGGTGGTAA TTTTTCTGGG GTCTTTCTAT 
CTGATCAACT TGATCCTTGC TGTGGTGGCC ATGGCATATG CAGAACAGAA TGAAGCCACT 
CTGCAAGAAG AAATAGAAAA GGAGAAAGAG TTCCAGGAGC TTTTGTTGCA GCTAAAGAAA 
CATCAAGAAG AGCAAGCAAG AAGGAAATCC AGCGTTGCCT CAGTTGGACT TCTGAACGCA 
GAAAAGAAAA AAGCTCTTTC AGAGAACACT CTTTCTGGGG ATGGCACAGA AAATATGGAC 
TGCAATGGGC GAACTGTGCC CAAAGTTATA ATTGAACGTG CACCCTCAAT GGAGGATACA 
AGCTCAGCAC AGTCAGACCA ACAGAGGAAT TCTAAAGTTT ATTTGGAGGA GCCAACTCTG 
CAGAAAAGAG CAGAGAGCAC CCACAGTGTC CTATCGGAAG TAGCTCTTGA AGAACTGGAA 
GAGGCTCATC AGAAATGCCC TCCTTGGTGG TACAAGTTTG CTAACACCTT TTTGATTTGG 
GACTGCTGTG ACCTGTGGGT GAAATTTAAG GAATTTGTAA GGTTTGTGGT CATGGATCCC 
TTTGTTGACC TGGGAATCAC CATTTGCATC GTGCTTAACA CCCTTTTTAT GGCCATGGAG 
CACTACCCAA TGACTGAGCA GTTTGAGGGT GTTCTGAATG TAGGAAACTT GGTCTTTACT 
GGCATATTTG CAGCTGAAAT GTTCTTCAAG ATCATAGCCC TGGATCCATA TTACTACTTC 
CAAGTGGGGT GGAACATATT CGACAGCATT ATAGTCACTT TAAGTTTGGT GGAGTTGGGC 
CTTGCAAATG TGGAAGGTCT GTCCGTGCTC AGATCGTTCC GTCTGCTCAG GGTGTTCAAG 
CTAGCCAAAT CATGGCCAAC CCTTAACATG CTTATCAAAA TCATCGGTAA CTCGGTCGGA 
GCACTTGGAA ACCTCACCTT GGTGTTGGCC ATCATTGTCT TCATCTTTGC GGTGGTGGGG 
ATGCAGCTAT TTGGGAAGAG CTACAAGGAA TGTGTGTGCA AGATTGCCTC TGACTGCGAG 
CTACCACGGT GGCACATGCA CGATTTCTTC CACTCTTTTC TCATTGTCTT CCGTATCCTG 
TGCGGGGAGT GGATAGAGAC AATGTGGGAC TGCATGGAAG TGGCAGGGCA AGCAATGTGC 
CTGGTCGTGT TCATGATGGT CATGGTGATT GGTAACCTGG TGGTCCTCAA TCTCTTTTTG 
GCTTTGTTGC TGAGTTCCTT CAGCGCGGAT AACTTGTCGG CTTCGGACGA CGATGGGGAG 
ATGAACAACC TGCAGATCGC CATCGGAAGG ATCACCAGGG GCATTGACTT TGTGAAGAAA 
ACCTTCTTCT CCATCCTACA TTACAAGAGG AAGGCAAAAG CTGAGCAGGA GGAAGAGGAC 
CCTAACAAAG AAAACATTGC TCTGAACCAT GTAGAGACAG AACCAGAACC CAAGATAGAA 
CTAGAACCCA GGACAGAACC AGAACCCAAA AAAGAACCTC CACTGATTGA TGGCGTGACT 
AAAAAACGTT ATTACATGGA TGAGTTTGAC CACGCTAATT TTTTTAACAA CCCACACATG 
AGAATAGAAG TACCTATTGC CTCAGAAGAG TCCGACTTCG ACATTCCAGA CACGGATGAG 
TTTAGCACAC AGTCGGAGGT AGAGGAAGGG ATTTTACACC ACAAGAAGTA CGATGATGGC 
TCGTCAGTGT GTAGCACAGC AGATTATAAA CCTCCTGAAC CTGAAGAGGA AGAGGAGGCC 
TTTGAACACA AAGAAGACGA AGGCCCAGAA GAATGCTTTA CTGCTGACTG TGTACGAAGA 
TGCCCTTGTC TTTATGTCGA CATCACAACA GAAAAAGGGA AAATTTGGTG GAACATTCGG 
AAAACATGTT TCCAGATTGT GGAGCATAAC TGGTTTGAAA CCTTCATCAT CTTCATGATC 
TTACTCAGTA GTGGAGCACT GGCATTTGAG GATATCTACA TTGAACAGAG GCAAGTGATA 
AAGACTATCC TGGAGTACGC TGATAAAGTC TTCACATATA TTTTTGTGTT TGAAATGCTG 
CTAAAGTGGA CAGCGTATGG CTTCAAGATC TATTTCACGA ATGCCTGGTG CTGGCTGGAC 
TTCCTAATTG TTGACGTGTC TTTAATCAGC CTGACAGCCA ACTGGCTGGG CTACTCGGAA 
CTTGGTCCCA TCAAATCCCT TAGAACGCTG AGGGCCCTTA GGCCGTTACG AGCGTTATCA 
AGATTTGAAG GCATGAGGGT GGTTGTAAAC GCCCTTCTGG GAGCCATTCC TTCTATCATG 
AATGTCCTGC TTGTTTGTCT GATCTTCTGG CTCATCTTCA GCATCATGGG TGTTAACCTC 
TTTGCTGGGA AATATTATCG ATGCATCAAC ACGACCACGT CAGAATTATT TGAGATCGAT 
GTGGTCAACA ACCGCTCGGA CTGCCTCGCT TTAAACAACT CCGAGCAAGT GCGATGGGTT 
AATGTCAAAG TGAATTTTGA CAATGTGGGC CTTGGGTATC TCTCCCTTCT ACAAGTGGCA 
ACCTTCAAAG GCTGGATGGA TATCATGTAT GCCGCTGTAG ATTCCCGGAA TATTGAGGAT 
CAGCCACAAT ATGAAATAAA TCTTTATATG TATTTGTACT TTGTAATATT CATTATTTTT 
GGAGCTTTCT TCACTCTAAA CCTGTTTATC GGTGTCATTA TCGACAACTT CAATCAGCAA 
AAGAAAAAGT TTGGAGGTAA GGATATCTTT ATGACTGAGG AACAGAAGAA ATACTACAAT 
GCTATGAAAA AGCTGGGATC CAAAAAACCC CAAAAGCCAA TTCCAAGACC CACGAACAAA 
ATTCAAGGTT TGGTGTATGA CTTTGTCACA AAGCAGCTCT TTGACATTGT GATTATGATT 
TTAATTTGCC TCAACATGGT GACGATGATG ATTGAAACAG ATGACCAGAG TCAGGAAAAA 
ACTGATATTC TTTTCAAGAT CAATCTGGTG TTCATTGTCA TCTTCACAGC CGAATGTGTT 
CTCAAAATGT TTGCGCTGAG GTACTACTTT TTCACAATAG GATGGAACAT ATTTGACTTT 
GTGGTGGTCA TCCTTTCTAT TGTTGGTATT GTTCTCTCGG ATCTCATAGC AGAGTACTTT 
GTTTCACCAA CACTTTTCAG AGTCATCAGA CTGGCGAGAA TCGGTAGAGT TCTTCGACTC 
ATTAGAGGAG CAAAGGGGAT TCGCACATTA CTTTTTGCCC TGATGATGTC TCTCCCCGCT 
CTCTTTAATA TCGGGCTTTT GCTTTTCTTA GTTATGTTCA TCTATTCAAT CTTTGGAATG 
TCCAATTTTG CCTATGTAAA GAAAGAATCT GGAATAGATG ACATATTTAA TTTTGAAACC 
TTTGGAAACA GCATAATATG CTTGTTCCAA ATCACCACGT CTGCTGGTTG GGATGGTCTT 
TTAAATCCAA TCCTAAATAG CGGCCCACCA GATTGTGACC CCCATCTGGA GAACTTTGGA 
ACAACAGTTA CTGGTGATTG TGGTAACCCA GCTATAGGTA TTGTGTTCTT CTGCAGCTAC 
ATCATAATAT CATTTTTAAT AGTTGTCAAT ATGTACATTG CCATTATCCT GGAGAACTTT 
AATGTTGCCA CAGAAGAGAG TGGTGAGCCA TTATGTGAGG ATGACTTTGA GATGTTTTAT 
GAAACTTGGG AGAAGTTTGA TCCAGATGCC ACTCAGTTTG TTGAATACAG TCGAATGTCA 
GACTTTGTAG ACACTTTGCA AGATCCTCTA CGGATTCCGA AACCTAACAA AATAAAACTA 
ATTACTTTGG ATCTCCCAAT GGTAACTGGA GATAAGATTC ACTGCTTGGA TATTCTGTTT 
GCACTAACTA AAGAGGTGTT GGGGGACTCA GGAGAAATGG ATGCATTAAA AGCCTCAATG 
GAAGAAAAGT TTATGATGGC AAATCCATCA AAAGTGTCTT ATGAACCAAT CACCACCACC 
CTTAAAAGAA AGCAGGAAGA AGTCTGTGTC ATCAAAATCC AGAGGGCCTT TAGGAGACAT 
CTATTGAGAC GTTCGGTGAA ACACGCTTCC TACATCTTCA GGCAAAGTAA TGATGAAGAG 
TCATCAGTGG AGGAACCTCC TGAAAAAGAA GGGTTGATAG CTGAAAGAAT TAATGCAGCC 
TACGGAAAAT ATGTAGAGGA AGGGCAAAAC GGCGGAACTC ATCGCCCAAG ATCAACAAAC 
AGAACTTCAC ACAGCAGTAT AGATTATGAT GACTCCAAAT CAAAAGCTCT TGCCTTCTTC 
CTGTTGGAAC TGACAGAGGG AACACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017944811.1
CDS	140..5689
Translation	MAGKVHPPGPDSLRPFTRESLAAIEKRIEEDIARKAKQIEVLDEKVPTPKNGLEAGKNLPLIYGDPPPELIGIPIEELDSFYSNQKTFIVLNKGKAIFRFTATKALYLLDPFNPIRRGAIKVLIHSLFSLFIMFTILTNCVFMTMSDPPPWSKNVEYTFTGIYTFESLIKILARGFCIDSFTFLRDPWNWLDFCVIVMAYTTEFVDLGNVSALRTFRVLRALKTITVIPGLKTIVGALIQSVKKLADVMILTLFCLAVFALIGLQLFMGNLRHKCVRWPPPDDYFMLYDNSTLNSTLYNDTMINSTLSNSTNVFDNDTIDWGAYLNDEANFYFLDGANDALLCGNSSDAGKCPESYTCMKTGRNPNYGYTSYDSFNWAFLSLFRLMTQDFWENLFQLTLRAAGKTYMVFFVVVIFLGSFYLINLILAVVAMAYAEQNEATLQEEIEKEKEFQELLLQLKKHQEEQARRKSSVASVGLLNAEKKKALSENTLSGDGTENMDCNGRTVPKVIIERAPSMEDTSSAQSDQQRNSKVYLEEPTLQKRAESTHSVLSEVALEELEEAHQKCPPWWYKFANTFLIWDCCDLWVKFKEFVRFVVMDPFVDLGITICIVLNTLFMAMEHYPMTEQFEGVLNVGNLVFTGIFAAEMFFKIIALDPYYYFQVGWNIFDSIIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKIASDCELPRWHMHDFFHSFLIVFRILCGEWIETMWDCMEVAGQAMCLVVFMMVMVIGNLVVLNLFLALLLSSFSADNLSASDDDGEMNNLQIAIGRITRGIDFVKKTFFSILHYKRKAKAEQEEEDPNKENIALNHVETEPEPKIELEPRTEPEPKKEPPLIDGVTKKRYYMDEFDHANFFNNPHMRIEVPIASEESDFDIPDTDEFSTQSEVEEGILHHKKYDDGSSVCSTADYKPPEPEEEEEAFEHKEDEGPEECFTADCVRRCPCLYVDITTEKGKIWWNIRKTCFQIVEHNWFETFIIFMILLSSGALAFEDIYIEQRQVIKTILEYADKVFTYIFVFEMLLKWTAYGFKIYFTNAWCWLDFLIVDVSLISLTANWLGYSELGPIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKYYRCINTTTSELFEIDVVNNRSDCLALNNSEQVRWVNVKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSRNIEDQPQYEINLYMYLYFVIFIIFGAFFTLNLFIGVIIDNFNQQKKKFGGKDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPTNKIQGLVYDFVTKQLFDIVIMILICLNMVTMMIETDDQSQEKTDILFKINLVFIVIFTAECVLKMFALRYYFFTIGWNIFDFVVVILSIVGIVLSDLIAEYFVSPTLFRVIRLARIGRVLRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMSNFAYVKKESGIDDIFNFETFGNSIICLFQITTSAGWDGLLNPILNSGPPDCDPHLENFGTTVTGDCGNPAIGIVFFCSYIIISFLIVVNMYIAIILENFNVATEESGEPLCEDDFEMFYETWEKFDPDATQFVEYSRMSDFVDTLQDPLRIPKPNKIKLITLDLPMVTGDKIHCLDILFALTKEVLGDSGEMDALKASMEEKFMMANPSKVSYEPITTTLKRKQEEVCVIKIQRAFRRHLLRRSVKHASYIFRQSNDEESSVEEPPEKEGLIAERINAAYGKYVEEGQNGGTHRPRSTNRTSHSSIDYDDSKSKALAFFLLELTEGTQ

Target ORF information:

RefSeq Version	XM_018089322.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sodium channel, voltage gated, type IV alpha subunit (scn4a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018089322.1

ORF Insert Sequence:

ATGGCTGGCA AGGTGCACCC TCCCGGTCCT GATAGTTTGC GTCCATTTAC CAGAGAGTCC 
TTGGCTGCGA TAGAGAAGCG CATTGAGGAG GACATCGCTC GGAAAGCCAA ACAGATTGAG 
GTGCTCGATG AGAAAGTACC GACACCCAAA AATGGACTGG AGGCAGGAAA GAACTTGCCT 
CTTATATATG GGGATCCCCC ACCCGAACTT ATTGGGATTC CCATAGAGGA ACTAGATTCT 
TTTTACAGCA ACCAAAAGAC ATTTATCGTC TTAAACAAAG GAAAAGCAAT ATTCCGATTC 
ACAGCCACCA AAGCCTTATA TCTACTGGAT CCATTTAATC CAATCAGAAG AGGCGCTATT 
AAAGTCTTAA TCCATTCATT GTTTAGCTTA TTTATTATGT TTACCATCTT AACAAATTGT 
GTTTTTATGA CCATGAGTGA CCCACCACCC TGGTCCAAGA ATGTAGAGTA CACGTTTACT 
GGAATTTACA CCTTTGAGTC CTTAATAAAA ATCCTGGCTC GGGGTTTTTG TATTGACAGT 
TTTACATTCC TCAGGGATCC GTGGAACTGG CTGGATTTCT GTGTCATTGT TATGGCTTAC 
ACCACAGAGT TTGTAGATTT GGGGAATGTG TCTGCCCTTA GGACATTCCG TGTACTCCGT 
GCTCTTAAAA CAATTACAGT TATTCCAGGC CTGAAGACTA TCGTTGGTGC CTTGATCCAA 
TCAGTGAAGA AGCTCGCAGA TGTGATGATC CTCACCCTCT TCTGCCTTGC TGTCTTTGCC 
CTCATTGGCC TTCAGCTCTT TATGGGAAAT CTGAGGCACA AATGTGTCCG ATGGCCACCC 
CCTGATGACT ATTTCATGCT TTATGACAAC AGTACCTTAA ACTCCACACT ATATAATGAT 
ACAATGATCA ACTCCACTCT CAGCAACAGC ACAAATGTGT TCGACAACGA CACCATTGAC 
TGGGGTGCCT ATCTCAACGA TGAAGCAAAT TTTTACTTCC TGGATGGAGC AAATGATGCA 
CTTCTGTGTG GGAACAGCAG TGATGCCGGG AAATGTCCAG AAAGTTACAC ATGTATGAAA 
ACTGGGAGAA ATCCTAATTA TGGGTACACC AGCTACGACT CATTCAACTG GGCTTTCCTT 
TCCCTTTTCC GATTAATGAC ACAGGACTTC TGGGAGAATC TTTTCCAGTT GACACTACGA 
GCAGCTGGTA AAACCTACAT GGTCTTCTTT GTGGTGGTAA TTTTTCTGGG GTCTTTCTAT 
CTGATCAACT TGATCCTTGC TGTGGTGGCC ATGGCATATG CAGAACAGAA TGAAGCCACT 
CTGCAAGAAG AAATAGAAAA GGAGAAAGAG TTCCAGGAGC TTTTGTTGCA GCTAAAGAAA 
CATCAAGAAG AGCAAGCAAG AAGGAAATCC AGCGTTGCCT CAGTTGGACT TCTGAACGCA 
GAAAAGAAAA AAGCTCTTTC AGAGAACACT CTTTCTGGGG ATGGCACAGA AAATATGGAC 
TGCAATGGGC GAACTGTGCC CAAAGTTATA ATTGAACGTG CACCCTCAAT GGAGGATACA 
AGCTCAGCAC AGTCAGACCA ACAGAGGAAT TCTAAAGTTT ATTTGGAGGA GCCAACTCTG 
CAGAAAAGAG CAGAGAGCAC CCACAGTGTC CTATCGGAAG TAGCTCTTGA AGAACTGGAA 
GAGGCTCATC AGAAATGCCC TCCTTGGTGG TACAAGTTTG CTAACACCTT TTTGATTTGG 
GACTGCTGTG ACCTGTGGGT GAAATTTAAG GAATTTGTAA GGTTTGTGGT CATGGATCCC 
TTTGTTGACC TGGGAATCAC CATTTGCATC GTGCTTAACA CCCTTTTTAT GGCCATGGAG 
CACTACCCAA TGACTGAGCA GTTTGAGGGT GTTCTGAATG TAGGAAACTT GGTCTTTACT 
GGCATATTTG CAGCTGAAAT GTTCTTCAAG ATCATAGCCC TGGATCCATA TTACTACTTC 
CAAGTGGGGT GGAACATATT CGACAGCATT ATAGTCACTT TAAGTTTGGT GGAGTTGGGC 
CTTGCAAATG TGGAAGGTCT GTCCGTGCTC AGATCGTTCC GTCTGCTCAG GGTGTTCAAG 
CTAGCCAAAT CATGGCCAAC CCTTAACATG CTTATCAAAA TCATCGGTAA CTCGGTCGGA 
GCACTTGGAA ACCTCACCTT GGTGTTGGCC ATCATTGTCT TCATCTTTGC GGTGGTGGGG 
ATGCAGCTAT TTGGGAAGAG CTACAAGGAA TGTGTGTGCA AGATTGCCTC TGACTGCGAG 
CTACCACGGT GGCACATGCA CGATTTCTTC CACTCTTTTC TCATTGTCTT CCGTATCCTG 
TGCGGGGAGT GGATAGAGAC AATGTGGGAC TGCATGGAAG TGGCAGGGCA AGCAATGTGC 
CTGGTCGTGT TCATGATGGT CATGGTGATT GGTAACCTGG TGGTCCTCAA TCTCTTTTTG 
GCTTTGTTGC TGAGTTCCTT CAGCGCGGAT AACTTGTCGG CTTCGGACGA CGATGGGGAG 
ATGAACAACC TGCAGATCGC CATCGGAAGG ATCACCAGGG GCATTGACTT TGTGAAGAAA 
ACCTTCTTCT CCATCCTACA TTACAAGAGG AAGGCAAAAG CTGAGCAGGA GGAAGAGGAC 
CCTAACAAAG AAAACATTGC TCTGAACCAT GTAGAGACAG AACCAGAACC CAAGATAGAA 
CTAGAACCCA GGACAGAACC AGAACCCAAA AAAGAACCTC CACTGATTGA TGGCGTGACT 
AAAAAACGTT ATTACATGGA TGAGTTTGAC CACGCTAATT TTTTTAACAA CCCACACATG 
AGAATAGAAG TACCTATTGC CTCAGAAGAG TCCGACTTCG ACATTCCAGA CACGGATGAG 
TTTAGCACAC AGTCGGAGGT AGAGGAAGGG ATTTTACACC ACAAGAAGTA CGATGATGGC 
TCGTCAGTGT GTAGCACAGC AGATTATAAA CCTCCTGAAC CTGAAGAGGA AGAGGAGGCC 
TTTGAACACA AAGAAGACGA AGGCCCAGAA GAATGCTTTA CTGCTGACTG TGTACGAAGA 
TGCCCTTGTC TTTATGTCGA CATCACAACA GAAAAAGGGA AAATTTGGTG GAACATTCGG 
AAAACATGTT TCCAGATTGT GGAGCATAAC TGGTTTGAAA CCTTCATCAT CTTCATGATC 
TTACTCAGTA GTGGAGCACT GGCATTTGAG GATATCTACA TTGAACAGAG GCAAGTGATA 
AAGACTATCC TGGAGTACGC TGATAAAGTC TTCACATATA TTTTTGTGTT TGAAATGCTG 
CTAAAGTGGA CAGCGTATGG CTTCAAGATC TATTTCACGA ATGCCTGGTG CTGGCTGGAC 
TTCCTAATTG TTGACGTGTC TTTAATCAGC CTGACAGCCA ACTGGCTGGG CTACTCGGAA 
CTTGGTCCCA TCAAATCCCT TAGAACGCTG AGGGCCCTTA GGCCGTTACG AGCGTTATCA 
AGATTTGAAG GCATGAGGGT GGTTGTAAAC GCCCTTCTGG GAGCCATTCC TTCTATCATG 
AATGTCCTGC TTGTTTGTCT GATCTTCTGG CTCATCTTCA GCATCATGGG TGTTAACCTC 
TTTGCTGGGA AATATTATCG ATGCATCAAC ACGACCACGT CAGAATTATT TGAGATCGAT 
GTGGTCAACA ACCGCTCGGA CTGCCTCGCT TTAAACAACT CCGAGCAAGT GCGATGGGTT 
AATGTCAAAG TGAATTTTGA CAATGTGGGC CTTGGGTATC TCTCCCTTCT ACAAGTGGCA 
ACCTTCAAAG GCTGGATGGA TATCATGTAT GCCGCTGTAG ATTCCCGGAA TATTGAGGAT 
CAGCCACAAT ATGAAATAAA TCTTTATATG TATTTGTACT TTGTAATATT CATTATTTTT 
GGAGCTTTCT TCACTCTAAA CCTGTTTATC GGTGTCATTA TCGACAACTT CAATCAGCAA 
AAGAAAAAGT TTGGAGGTAA GGATATCTTT ATGACTGAGG AACAGAAGAA ATACTACAAT 
GCTATGAAAA AGCTGGGATC CAAAAAACCC CAAAAGCCAA TTCCAAGACC CACGAACAAA 
ATTCAAGGTT TGGTGTATGA CTTTGTCACA AAGCAGCTCT TTGACATTGT GATTATGATT 
TTAATTTGCC TCAACATGGT GACGATGATG ATTGAAACAG ATGACCAGAG TCAGGAAAAA 
ACTGATATTC TTTTCAAGAT CAATCTGGTG TTCATTGTCA TCTTCACAGC CGAATGTGTT 
CTCAAAATGT TTGCGCTGAG GTACTACTTT TTCACAATAG GATGGAACAT ATTTGACTTT 
GTGGTGGTCA TCCTTTCTAT TGTTGGTATT GTTCTCTCGG ATCTCATAGC AGAGTACTTT 
GTTTCACCAA CACTTTTCAG AGTCATCAGA CTGGCGAGAA TCGGTAGAGT TCTTCGACTC 
ATTAGAGGAG CAAAGGGGAT TCGCACATTA CTTTTTGCCC TGATGATGTC TCTCCCCGCT 
CTCTTTAATA TCGGGCTTTT GCTTTTCTTA GTTATGTTCA TCTATTCAAT CTTTGGAATG 
TCCAATTTTG CCTATGTAAA GAAAGAATCT GGAATAGATG ACATATTTAA TTTTGAAACC 
TTTGGAAACA GCATAATATG CTTGTTCCAA ATCACCACGT CTGCTGGTTG GGATGGTCTT 
TTAAATCCAA TCCTAAATAG CGGCCCACCA GATTGTGACC CCCATCTGGA GAACTTTGGA 
ACAACAGTTA CTGGTGATTG TGGTAACCCA GCTATAGGTA TTGTGTTCTT CTGCAGCTAC 
ATCATAATAT CATTTTTAAT AGTTGTCAAT ATGTACATTG CCATTATCCT GGAGAACTTT 
AATGTTGCCA CAGAAGAGAG TGGTGAGCCA TTATGTGAGG ATGACTTTGA GATGTTTTAT 
GAAACTTGGG AGAAGTTTGA TCCAGATGCC ACTCAGTTTG TTGAATACAG TCGAATGTCA 
GACTTTGTAG ACACTTTGCA AGATCCTCTA CGGATTCCGA AACCTAACAA AATAAAACTA 
ATTACTTTGG ATCTCCCAAT GGTAACTGGA GATAAGATTC ACTGCTTGGA TATTCTGTTT 
GCACTAACTA AAGAGGTGTT GGGGGACTCA GGAGAAATGG ATGCATTAAA AGCCTCAATG 
GAAGAAAAGT TTATGATGGC AAATCCATCA AAAGTGTCTT ATGAACCAAT CACCACCACC 
CTTAAAAGAA AGCAGGAAGA AGTCTGTGTC ATCAAAATCC AGAGGGCCTT TAGGAGACAT 
CTATTGAGAC GTTCGGTGAA ACACGCTTCC TACATCTTCA GGCAAAGTAA TGATGAAGAG 
TCATCAGTGG AGGAACCTCC TGAAAAAGAA GGGTTGATAG CTGAAAGAAT TAATGCAGCC 
TACGGAAAAT ATGTAGAGGA AGGGCAAAAC GGCGGAACTC ATCGCCCAAG ATCAACAAAC 
AGAACTTCAC ACAGCAGTAT AGATTATGAT GACTCCAAAT CAAAAGCTCT TGCCTTCTTC 
CTGTTGGAAC TGACAGAGGG AACACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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scn4a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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