robo2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

  • Gene

  • Clones

  • gRNAs

The following robo2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the robo2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa51982 XM_031896605.1
Latest version!
Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa51983 XM_031896606.1
Latest version!
Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa51970 XM_031896593.1
Latest version!
Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa51971 XM_031896594.1
Latest version!
Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa51972 XM_031896595.1
Latest version!
Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa51973 XM_031896596.1
Latest version!
Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa51974 XM_031896597.1
Latest version!
Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa51975 XM_031896598.1
Latest version!
Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa51976 XM_031896599.1
Latest version!
Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa51977 XM_031896600.1
Latest version!
Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa51978 XM_031896601.1
Latest version!
Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa51979 XM_031896602.1
Latest version!
Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa51980 XM_031896603.1
Latest version!
Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa51981 XM_031896604.1
Latest version!
Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa06334 XM_004912079.3
Latest version!
Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa06335 XM_004912080.3
Latest version!
Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa06336 XM_002931767.4
Latest version!
Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa35813 XM_012957961.2
Latest version!
Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa35814 XM_012957962.2
Latest version!
Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa35815 XM_012957963.2
Latest version!
Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa35816 XM_012957964.2
Latest version!
Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa35817 XM_012957965.2
Latest version!
Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa35818 XM_012957966.2
Latest version!
Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa35819 XM_012957967.2
Latest version!
Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa51982
    Clone ID Related Accession (Same CDS sequence) XM_031896605.1
    Accession Version XM_031896605.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4458bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product roundabout homolog 2 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAACATCT GGATAATGCT ATCTTCACTA AGGCAGAGGG CAGCTTCTCC TGAATTCCTG 
    CTGATCCTTA TGCTGTTCGT CAGTGGGGTG ACCCAAAGCA AAATTAGTAG GACCAAGGGG
    AAGCACTGGA AAGGGGGATC CCGTCTCCGT CAAGAAGATT TCCCCCCACG GATAGTTGAG
    CATCCATCGG ATGTCATTGT GTCAAAGGGA GAGCCAACCA CCTTGAACTG CAAAGCGGAA
    GGGCGGCCTA CACCCACCAT AGAGTGGTAC AAAGACTTAG AAAGGGTTGA GACAGACAAG
    GATGACCCCC GCTCACATCG AATGCTACTG CCAAGTGGAT CTTTATTTTT TCTGCGCATT
    ATCCATGGGC GCAAGTTTAA GCCAGACGAA GGAAGTTACG TCTGTGTGGC AAGGAACTAT
    CTCGGGGAGG CTATGAGTCG AAATGCATCT CTGGAAGTGG CATTGCTCCG TGATGACTTC
    CGGCTCAACC CTACAGATGT TGTTGTGGCA GCTGGGGAAC CTGCAATTTT GGAGTGTCAA
    CCCCCCAGAG GACATCCGGA ACCAACTATA TACTGGAAAA AGGACAAAGT TAGAATTGAT
    GATAAAGATG AAAGAATTAG TATTCGAGGT GGAAAGCTGA TGCTCTCCAA TACAAGGAAA
    AGTGATGCAG GCATGTACAC TTGTGTTGGG ACCAACATGG TTGGAGAAAG AGACAGTGAC
    CCAGCAGAGC TAACAGTCTT TGAACGTCCC ACATTCCTCA GAAGGCCAAT TAATCAAGTT
    GTTTTAGAGG ATGAAGCTGT AGAATTTCGA TGTCAGGTCC AGGGGGACCC ACAGCCAACT
    GTCCGGTGGA AAAAGGATGA TGTAGATTTG CCAAGAGGAA GATACGACAT CAAGGATGAC
    TATACTTTGA GAATCAAGAA GGCTTCCAGC AGCGACGAAG GGACCTTTGC TTGTATTTCT
    GAAAACCGGG TTGGTAAAAT AGAAGCTTCT GCCACACTTA CAGTCAGAGT TCGCCCTGTT
    GCTCCTCCCC AGTTTGTGAT CAGACCAAGG GATCAGATTG TAGCACAAGG CCGGACAGTA
    ACATTTGGAT GTGAAGCTAA AGGAAATCCT CAACCAGCTG TTTTTTGGCA AAAAGAAGGA
    AGCCAGAATT TACTTTTTCC AAATCAGCCT CTTCAGCCCA ACAGCAGGTA CTCAGTGTCA
    CCAACTGGAG ATTTAACCAT CACCAGCATT CAGCGCTCAG ACGCAGGCTA CTACATTTGT
    CAGGCACTGA CTGTTGCCGG AAGCATTCTG GCCAAGGCCC AGCTGGAAGT GACTGATGTT
    TTGACAGATA GACCACCGCC AATAATTCTA CAAGGTCCAG CTAATCAAAC TCTTGCCGTG
    GATGGAACAG CAGTGCTGAA ATGTAAAACT ACTGGGGACC CAATGCCAGT CATAAGTTGG
    CTAAAAGAGG GATTTAAGGG ATTCATAGGG AGAGATGCAA GGTTATCCAT TCTAGACCAA
    GGGACATTAC AAATTAAAGG TTTAAAGGTT ACTGATTCTG GCACCTATAC TTGTGTGGCT
    ACTAGCTCTA GTGGTGAGAC AACATGGAGC GCTGTTCTGG AAGTGACAGA ATCTGGTGGT
    GCCATAATTA GCAAGAACTA TGATCTCAAT GACCTTCCTG GATCTCCATC AAAACCTCAG
    GTTACAGATG TCAGTAAGAA CAGTGTTACC CTATCATGGC AACCAGGATC ACCAGGGATT
    CTGCCTGTGA CATCCTACAT CATTGAGGCT TTCAGTCAAT CTGTGAGCAA TAGTTGGCAA
    ACTGTGGCCA ATCACGTGAA GACAACGCTT TACACTGTTA AAGGACTGCG CCCAAACACC
    ATTTATCTCT TTATGGTGAG AGCAATCAAT ACTGAAGGAC TGAGTGACCC AAGTCCAATA
    TCTGATCCAG TGCGTACACA AGATATAAGT CCACCTGTTC AAGGAGTAGA TCACCGGCAA
    GTACAGAAAG AACTTGGGGA TGTGATTGTA CGGCTCCATA ATCCTGTTGT TCTGACACCA
    ACAACAATCC AGGTCACCTG GATGGTCGAC CGTCAATCCC AGTTTATTCA GGGATACAGG
    GTAATGTACC GTCAGACTTC TGGTCTGCAG ACTCCATCTG CGTGGCAGAG CTTAGAGGTC
    AAAGTGCCTG CAGAGCGTAG CGCAGTGCTA ACCAACCTGA AAAAAGGAAT TACTTATGAG
    ATTAAGGTTC GACCTTATTT TAATGAATTT CAAGGAATGG ATAGCGAGTC AAAGTCAGCA
    CGAACAACTG AAGAAGCCCC AAGTGCTCCT CCTCAGTCTG TTACTGTTAT GACAGTGGGA
    AATAACAACA GCACCAGCAT CAGCATCTCA TGGGACCCCC CTCCATCTGA CCACCAGAAT
    GGAATTATCC AGGACTACAG GATCTGGTGC CTGGGTAATG AGACACGTTT CCACATCAAC
    AAGACAGTGG ATGCTGCTAT TCGTTCCGTG GTTATCGGTG GGCTATTCCC TGGAATTCAG
    TACCGCGTTG AAGTCGCAGC CAGTACAAGC GCAGGCACTG GGGTAAAAAG TGCTCCCCAG
    CACATACAGA TAGGAAATCG GAATGAAGTT GTGATCACAG AGAATAACAA CAGCATAACA
    GAGCAAATAA CTGATGTTGT GAAACAGCCG GCATTCATCG CAGGAATTGG TGGTGCCTGC
    TGGGTAATAC TCATGGGATT TAGCATTTGG CTCTACTGGC GAAGAAAGAA GAGAAAGGGT
    CTCAGCAACT ACGCTGTTAC ATTCCAGAGA GGAGATGGAG GACTAATGAG TAATGGAAGT
    CGTCCTGGAC TTTTAAATGC GAATGATCCT AGTTACCCTT GGTTGGCAGA CTCCTGGCCA
    GCTTCCAGCA TGCCGGTGAA TAACAGCAAC AATGGACCAA ACGATCTCGT TAATTTTAGT
    TGTGGAGATG TGCTGCCAAC AGTTCCAGGT CAAGGAGAAA AAACAGCGAC TATGCTTTCT
    GATGGAGCCA TTTATAGCAG CATTGATTTT ACTACAAAAT CTAGCTATAA TTGCCCAGGA
    CAAATTGCAC AAACCACGCC ATATGCTACT ACGCAGATAC TACATTCCAA CAGCATTCAT
    GAGCTGGCAG TTGACTTGCC TGATCCACAG TGGAAATCTT CCATTCAGCA GAAATCTGAT
    CTCACTGGAT TAGGCTATTG CCTTACTGAC CAAGGCAAAG GGAGTAATGG TGGAAAAGAC
    GGAAAAAGGA AGAAAAATAA AAACAGCTCT AAAATCTCTA AAAACAATGG ATCCAGTTGG
    GCCAATGTTC CACTTCCGCC CCCTCCCATC CAACCACTGC CAGGCACTGA AATAGAACAT
    TATGGCCTTG AACACCAGGA AACAGGCTAT GACACCAATG GATGGTGCCC ACCTTTGCCA
    GTCCAGACCT ACTTACATCA AGGATCAGAT GATGAACTAG AAGAAGATGA AGAGAGAGTC
    CCTACACCCC CTGTACGTGG GGTGGCATCA TCACCTGCCA TCTCATTTGG ACAACAGTCA
    ACAGCAACCC TCACACCATC ACCACGTGAA GAAATGCAAC CCATGCTTCA GGCCCACCTG
    GATGAATTAA CAAGAGCTTA CCAGTTTGAC ATTGCAAAGC AATCTTGGCA TTTACACAGC
    AATAACCAGC CCCCATTGGC CCCAGTTCCA CCACTTGGAT ATGTGTCTGG GACATTTATT
    TCTGACTTAG AAAAAGACAT TCCAGATCAA GAAGAAGATG AAATTGAAGA CGAATCTATA
    GAAATAACAA GGCCCCTCCG AGGTTTGGAA CACACTCCCG GTTCTAGTAT GGATAATCTC
    GACAGTTCTG TGACAGGCAA AATGAACTCC TTTTCTCAGA GACCCCGGCC TGCCAGTCCC
    TTTTCTACTG ACAGTAACAC AAGTTCAGCT GTGAATCATA GTCAGAAGTC ACGGCCCACT
    AAGAAACACA AGGGAGGCAG AATAGGTCAG CAACCCATGC TTCGCAGAGA AGGGATTGCA
    GATGATTTGC CTCCCCCACC TGATCCTCCA CCTTGTCAAA GACAGCAAAT CATGCCCAGT
    CAACGTGGTG GAGGTCCTAC AGAAAGAAAA GCCAGTTCTC TTGAGAGGCA CACATCAAAT
    ATGAATGATT TAAAAACCTC ATTAGACCGG CATGGCAGAA CATCCTTAGA ACGGCAGCGA
    CAAACACAGG AATGGCTTGG CTCAACAGAA AGGCAGAACA AGCAGGCATT TGGATCTGAA
    GAGATACTAA TGCCATACAG TAAACCTAGC TTCCCATCTC CAGGAGGGCA CAGCTCCTCA
    GGAACAGTCT CTTCAAAAGA ATCAACTGGA CCCAGAAAAG GGGAGGTTTT GAGAGGTCAC
    CAACGTAATG CCATTGACTT TCTTGATGTA GGATATTTGG GGCCACACAG TCAAGGACAG
    TTTACAGGTG AATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752465.1
    CDS542..4999
    Translation

    Target ORF information:

    RefSeq Version XM_031896605.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896605.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAACATCT GGATAATGCT ATCTTCACTA AGGCAGAGGG CAGCTTCTCC TGAATTCCTG 
    CTGATCCTTA TGCTGTTCGT CAGTGGGGTG ACCCAAAGCA AAATTAGTAG GACCAAGGGG
    AAGCACTGGA AAGGGGGATC CCGTCTCCGT CAAGAAGATT TCCCCCCACG GATAGTTGAG
    CATCCATCGG ATGTCATTGT GTCAAAGGGA GAGCCAACCA CCTTGAACTG CAAAGCGGAA
    GGGCGGCCTA CACCCACCAT AGAGTGGTAC AAAGACTTAG AAAGGGTTGA GACAGACAAG
    GATGACCCCC GCTCACATCG AATGCTACTG CCAAGTGGAT CTTTATTTTT TCTGCGCATT
    ATCCATGGGC GCAAGTTTAA GCCAGACGAA GGAAGTTACG TCTGTGTGGC AAGGAACTAT
    CTCGGGGAGG CTATGAGTCG AAATGCATCT CTGGAAGTGG CATTGCTCCG TGATGACTTC
    CGGCTCAACC CTACAGATGT TGTTGTGGCA GCTGGGGAAC CTGCAATTTT GGAGTGTCAA
    CCCCCCAGAG GACATCCGGA ACCAACTATA TACTGGAAAA AGGACAAAGT TAGAATTGAT
    GATAAAGATG AAAGAATTAG TATTCGAGGT GGAAAGCTGA TGCTCTCCAA TACAAGGAAA
    AGTGATGCAG GCATGTACAC TTGTGTTGGG ACCAACATGG TTGGAGAAAG AGACAGTGAC
    CCAGCAGAGC TAACAGTCTT TGAACGTCCC ACATTCCTCA GAAGGCCAAT TAATCAAGTT
    GTTTTAGAGG ATGAAGCTGT AGAATTTCGA TGTCAGGTCC AGGGGGACCC ACAGCCAACT
    GTCCGGTGGA AAAAGGATGA TGTAGATTTG CCAAGAGGAA GATACGACAT CAAGGATGAC
    TATACTTTGA GAATCAAGAA GGCTTCCAGC AGCGACGAAG GGACCTTTGC TTGTATTTCT
    GAAAACCGGG TTGGTAAAAT AGAAGCTTCT GCCACACTTA CAGTCAGAGT TCGCCCTGTT
    GCTCCTCCCC AGTTTGTGAT CAGACCAAGG GATCAGATTG TAGCACAAGG CCGGACAGTA
    ACATTTGGAT GTGAAGCTAA AGGAAATCCT CAACCAGCTG TTTTTTGGCA AAAAGAAGGA
    AGCCAGAATT TACTTTTTCC AAATCAGCCT CTTCAGCCCA ACAGCAGGTA CTCAGTGTCA
    CCAACTGGAG ATTTAACCAT CACCAGCATT CAGCGCTCAG ACGCAGGCTA CTACATTTGT
    CAGGCACTGA CTGTTGCCGG AAGCATTCTG GCCAAGGCCC AGCTGGAAGT GACTGATGTT
    TTGACAGATA GACCACCGCC AATAATTCTA CAAGGTCCAG CTAATCAAAC TCTTGCCGTG
    GATGGAACAG CAGTGCTGAA ATGTAAAACT ACTGGGGACC CAATGCCAGT CATAAGTTGG
    CTAAAAGAGG GATTTAAGGG ATTCATAGGG AGAGATGCAA GGTTATCCAT TCTAGACCAA
    GGGACATTAC AAATTAAAGG TTTAAAGGTT ACTGATTCTG GCACCTATAC TTGTGTGGCT
    ACTAGCTCTA GTGGTGAGAC AACATGGAGC GCTGTTCTGG AAGTGACAGA ATCTGGTGGT
    GCCATAATTA GCAAGAACTA TGATCTCAAT GACCTTCCTG GATCTCCATC AAAACCTCAG
    GTTACAGATG TCAGTAAGAA CAGTGTTACC CTATCATGGC AACCAGGATC ACCAGGGATT
    CTGCCTGTGA CATCCTACAT CATTGAGGCT TTCAGTCAAT CTGTGAGCAA TAGTTGGCAA
    ACTGTGGCCA ATCACGTGAA GACAACGCTT TACACTGTTA AAGGACTGCG CCCAAACACC
    ATTTATCTCT TTATGGTGAG AGCAATCAAT ACTGAAGGAC TGAGTGACCC AAGTCCAATA
    TCTGATCCAG TGCGTACACA AGATATAAGT CCACCTGTTC AAGGAGTAGA TCACCGGCAA
    GTACAGAAAG AACTTGGGGA TGTGATTGTA CGGCTCCATA ATCCTGTTGT TCTGACACCA
    ACAACAATCC AGGTCACCTG GATGGTCGAC CGTCAATCCC AGTTTATTCA GGGATACAGG
    GTAATGTACC GTCAGACTTC TGGTCTGCAG ACTCCATCTG CGTGGCAGAG CTTAGAGGTC
    AAAGTGCCTG CAGAGCGTAG CGCAGTGCTA ACCAACCTGA AAAAAGGAAT TACTTATGAG
    ATTAAGGTTC GACCTTATTT TAATGAATTT CAAGGAATGG ATAGCGAGTC AAAGTCAGCA
    CGAACAACTG AAGAAGCCCC AAGTGCTCCT CCTCAGTCTG TTACTGTTAT GACAGTGGGA
    AATAACAACA GCACCAGCAT CAGCATCTCA TGGGACCCCC CTCCATCTGA CCACCAGAAT
    GGAATTATCC AGGACTACAG GATCTGGTGC CTGGGTAATG AGACACGTTT CCACATCAAC
    AAGACAGTGG ATGCTGCTAT TCGTTCCGTG GTTATCGGTG GGCTATTCCC TGGAATTCAG
    TACCGCGTTG AAGTCGCAGC CAGTACAAGC GCAGGCACTG GGGTAAAAAG TGCTCCCCAG
    CACATACAGA TAGGAAATCG GAATGAAGTT GTGATCACAG AGAATAACAA CAGCATAACA
    GAGCAAATAA CTGATGTTGT GAAACAGCCG GCATTCATCG CAGGAATTGG TGGTGCCTGC
    TGGGTAATAC TCATGGGATT TAGCATTTGG CTCTACTGGC GAAGAAAGAA GAGAAAGGGT
    CTCAGCAACT ACGCTGTTAC ATTCCAGAGA GGAGATGGAG GACTAATGAG TAATGGAAGT
    CGTCCTGGAC TTTTAAATGC GAATGATCCT AGTTACCCTT GGTTGGCAGA CTCCTGGCCA
    GCTTCCAGCA TGCCGGTGAA TAACAGCAAC AATGGACCAA ACGATCTCGT TAATTTTAGT
    TGTGGAGATG TGCTGCCAAC AGTTCCAGGT CAAGGAGAAA AAACAGCGAC TATGCTTTCT
    GATGGAGCCA TTTATAGCAG CATTGATTTT ACTACAAAAT CTAGCTATAA TTGCCCAGGA
    CAAATTGCAC AAACCACGCC ATATGCTACT ACGCAGATAC TACATTCCAA CAGCATTCAT
    GAGCTGGCAG TTGACTTGCC TGATCCACAG TGGAAATCTT CCATTCAGCA GAAATCTGAT
    CTCACTGGAT TAGGCTATTG CCTTACTGAC CAAGGCAAAG GGAGTAATGG TGGAAAAGAC
    GGAAAAAGGA AGAAAAATAA AAACAGCTCT AAAATCTCTA AAAACAATGG ATCCAGTTGG
    GCCAATGTTC CACTTCCGCC CCCTCCCATC CAACCACTGC CAGGCACTGA AATAGAACAT
    TATGGCCTTG AACACCAGGA AACAGGCTAT GACACCAATG GATGGTGCCC ACCTTTGCCA
    GTCCAGACCT ACTTACATCA AGGATCAGAT GATGAACTAG AAGAAGATGA AGAGAGAGTC
    CCTACACCCC CTGTACGTGG GGTGGCATCA TCACCTGCCA TCTCATTTGG ACAACAGTCA
    ACAGCAACCC TCACACCATC ACCACGTGAA GAAATGCAAC CCATGCTTCA GGCCCACCTG
    GATGAATTAA CAAGAGCTTA CCAGTTTGAC ATTGCAAAGC AATCTTGGCA TTTACACAGC
    AATAACCAGC CCCCATTGGC CCCAGTTCCA CCACTTGGAT ATGTGTCTGG GACATTTATT
    TCTGACTTAG AAAAAGACAT TCCAGATCAA GAAGAAGATG AAATTGAAGA CGAATCTATA
    GAAATAACAA GGCCCCTCCG AGGTTTGGAA CACACTCCCG GTTCTAGTAT GGATAATCTC
    GACAGTTCTG TGACAGGCAA AATGAACTCC TTTTCTCAGA GACCCCGGCC TGCCAGTCCC
    TTTTCTACTG ACAGTAACAC AAGTTCAGCT GTGAATCATA GTCAGAAGTC ACGGCCCACT
    AAGAAACACA AGGGAGGCAG AATAGGTCAG CAACCCATGC TTCGCAGAGA AGGGATTGCA
    GATGATTTGC CTCCCCCACC TGATCCTCCA CCTTGTCAAA GACAGCAAAT CATGCCCAGT
    CAACGTGGTG GAGGTCCTAC AGAAAGAAAA GCCAGTTCTC TTGAGAGGCA CACATCAAAT
    ATGAATGATT TAAAAACCTC ATTAGACCGG CATGGCAGAA CATCCTTAGA ACGGCAGCGA
    CAAACACAGG AATGGCTTGG CTCAACAGAA AGGCAGAACA AGCAGGCATT TGGATCTGAA
    GAGATACTAA TGCCATACAG TAAACCTAGC TTCCCATCTC CAGGAGGGCA CAGCTCCTCA
    GGAACAGTCT CTTCAAAAGA ATCAACTGGA CCCAGAAAAG GGGAGGTTTT GAGAGGTCAC
    CAACGTAATG CCATTGACTT TCTTGATGTA GGATATTTGG GGCCACACAG TCAAGGACAG
    TTTACAGGTG AATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa51983
    Clone ID Related Accession (Same CDS sequence) XM_031896606.1
    Accession Version XM_031896606.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4446bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product roundabout homolog 2 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAACATCT GGATAATGCT ATCTTCACTA AGGCAGAGGG CAGCTTCTCC TGAATTCCTG 
    CTGATCCTTA TGCTGTTCGT CAGTGGGGTG ACCCAAAGCA AAATTAGTAG GACCAAGGGG
    AAGCACTGGA AAGGGGGATC CCGTCTCCGT CAAGAAGATT TCCCCCCACG GATAGTTGAG
    CATCCATCGG ATGTCATTGT GTCAAAGGGA GAGCCAACCA CCTTGAACTG CAAAGCGGAA
    GGGCGGCCTA CACCCACCAT AGAGTGGTAC AAAGACTTAG AAAGGGTTGA GACAGACAAG
    GATGACCCCC GCTCACATCG AATGCTACTG CCAAGTGGAT CTTTATTTTT TCTGCGCATT
    ATCCATGGGC GCAAGTTTAA GCCAGACGAA GGAAGTTACG TCTGTGTGGC AAGGAACTAT
    CTCGGGGAGG CTATGAGTCG AAATGCATCT CTGGAAGTGG CATTGCTCCG TGATGACTTC
    CGGCTCAACC CTACAGATGT TGTTGTGGCA GCTGGGGAAC CTGCAATTTT GGAGTGTCAA
    CCCCCCAGAG GACATCCGGA ACCAACTATA TACTGGAAAA AGGACAAAGT TAGAATTGAT
    GATAAAGATG AAAGAATTAG TATTCGAGGT GGAAAGCTGA TGCTCTCCAA TACAAGGAAA
    AGTGATGCAG GCATGTACAC TTGTGTTGGG ACCAACATGG TTGGAGAAAG AGACAGTGAC
    CCAGCAGAGC TAACAGTCTT TGAACGTCCC ACATTCCTCA GAAGGCCAAT TAATCAAGTT
    GTTTTAGAGG ATGAAGCTGT AGAATTTCGA TGTCAGGTCC AGGGGGACCC ACAGCCAACT
    GTCCGGTGGA AAAAGGATGA TGTAGATTTG CCAAGAGGAA GATACGACAT CAAGGATGAC
    TATACTTTGA GAATCAAGAA GGCTTCCAGC AGCGACGAAG GGACCTTTGC TTGTATTTCT
    GAAAACCGGG TTGGTAAAAT AGAAGCTTCT GCCACACTTA CAGTCAGAGC TCCTCCCCAG
    TTTGTGATCA GACCAAGGGA TCAGATTGTA GCACAAGGCC GGACAGTAAC ATTTGGATGT
    GAAGCTAAAG GAAATCCTCA ACCAGCTGTT TTTTGGCAAA AAGAAGGAAG CCAGAATTTA
    CTTTTTCCAA ATCAGCCTCT TCAGCCCAAC AGCAGGTACT CAGTGTCACC AACTGGAGAT
    TTAACCATCA CCAGCATTCA GCGCTCAGAC GCAGGCTACT ACATTTGTCA GGCACTGACT
    GTTGCCGGAA GCATTCTGGC CAAGGCCCAG CTGGAAGTGA CTGATGTTTT GACAGATAGA
    CCACCGCCAA TAATTCTACA AGGTCCAGCT AATCAAACTC TTGCCGTGGA TGGAACAGCA
    GTGCTGAAAT GTAAAACTAC TGGGGACCCA ATGCCAGTCA TAAGTTGGCT AAAAGAGGGA
    TTTAAGGGAT TCATAGGGAG AGATGCAAGG TTATCCATTC TAGACCAAGG GACATTACAA
    ATTAAAGGTT TAAAGGTTAC TGATTCTGGC ACCTATACTT GTGTGGCTAC TAGCTCTAGT
    GGTGAGACAA CATGGAGCGC TGTTCTGGAA GTGACAGAAT CTGGTGGTGC CATAATTAGC
    AAGAACTATG ATCTCAATGA CCTTCCTGGA TCTCCATCAA AACCTCAGGT TACAGATGTC
    AGTAAGAACA GTGTTACCCT ATCATGGCAA CCAGGATCAC CAGGGATTCT GCCTGTGACA
    TCCTACATCA TTGAGGCTTT CAGTCAATCT GTGAGCAATA GTTGGCAAAC TGTGGCCAAT
    CACGTGAAGA CAACGCTTTA CACTGTTAAA GGACTGCGCC CAAACACCAT TTATCTCTTT
    ATGGTGAGAG CAATCAATAC TGAAGGACTG AGTGACCCAA GTCCAATATC TGATCCAGTG
    CGTACACAAG ATATAAGTCC ACCTGTTCAA GGAGTAGATC ACCGGCAAGT ACAGAAAGAA
    CTTGGGGATG TGATTGTACG GCTCCATAAT CCTGTTGTTC TGACACCAAC AACAATCCAG
    GTCACCTGGA TGGTCGACCG TCAATCCCAG TTTATTCAGG GATACAGGGT AATGTACCGT
    CAGACTTCTG GTCTGCAGAC TCCATCTGCG TGGCAGAGCT TAGAGGTCAA AGTGCCTGCA
    GAGCGTAGCG CAGTGCTAAC CAACCTGAAA AAAGGAATTA CTTATGAGAT TAAGGTTCGA
    CCTTATTTTA ATGAATTTCA AGGAATGGAT AGCGAGTCAA AGTCAGCACG AACAACTGAA
    GAAGCCCCAA GTGCTCCTCC TCAGTCTGTT ACTGTTATGA CAGTGGGAAA TAACAACAGC
    ACCAGCATCA GCATCTCATG GGACCCCCCT CCATCTGACC ACCAGAATGG AATTATCCAG
    GACTACAGGA TCTGGTGCCT GGGTAATGAG ACACGTTTCC ACATCAACAA GACAGTGGAT
    GCTGCTATTC GTTCCGTGGT TATCGGTGGG CTATTCCCTG GAATTCAGTA CCGCGTTGAA
    GTCGCAGCCA GTACAAGCGC AGGCACTGGG GTAAAAAGTG CTCCCCAGCA CATACAGATA
    GGAAATCGGA ATGAAGTTGT GATCACAGAG AATAACAACA GCATAACAGA GCAAATAACT
    GATGTTGTGA AACAGCCGGC ATTCATCGCA GGAATTGGTG GTGCCTGCTG GGTAATACTC
    ATGGGATTTA GCATTTGGCT CTACTGGCGA AGAAAGAAGA GAAAGGGTCT CAGCAACTAC
    GCTGTTACAT TCCAGAGAGG AGATGGAGGA CTAATGAGTA ATGGAAGTCG TCCTGGACTT
    TTAAATGCGA ATGATCCTAG TTACCCTTGG TTGGCAGACT CCTGGCCAGC TTCCAGCATG
    CCGGTGAATA ACAGCAACAA TGGACCAAAC GATCTCGTTA ATTTTAGTTG TGGAGATGTG
    CTGCCAACAG TTCCAGGTCA AGGAGAAAAA ACAGCGACTA TGCTTTCTGA TGGAGCCATT
    TATAGCAGCA TTGATTTTAC TACAAAATCT AGCTATAATT GCCCAGGACA AATTGCACAA
    ACCACGCCAT ATGCTACTAC GCAGATACTA CATTCCAACA GCATTCATGA GCTGGCAGTT
    GACTTGCCTG ATCCACAGTG GAAATCTTCC ATTCAGCAGA AATCTGATCT CACTGGATTA
    GGCTATTGCC TTACTGACCA AGGCAAAGGG AGTAATGGTG GAAAAGACGG AAAAAGGAAG
    AAAAATAAAA ACAGCTCTAA AATCTCTAAA AACAATGGAT CCAGTTGGGC CAATGTTCCA
    CTTCCGCCCC CTCCCATCCA ACCACTGCCA GGCACTGAAA TAGAACATTA TGGCCTTGAA
    CACCAGGAAA CAGGCTATGA CACCAATGGA TGGTGCCCAC CTTTGCCAGT CCAGACCTAC
    TTACATCAAG GATCAGATGA TGAACTAGAA GAAGATGAAG AGAGAGTCCC TACACCCCCT
    GTACGTGGGG TGGCATCATC ACCTGCCATC TCATTTGGAC AACAGTCAAC AGCAACCCTC
    ACACCATCAC CACGTGAAGA AATGCAACCC ATGCTTCAGG CCCACCTGGA TGAATTAACA
    AGAGCTTACC AGTTTGACAT TGCAAAGCAA TCTTGGCATT TACACAGCAA TAACCAGCCC
    CCATTGGCCC CAGTTCCACC ACTTGGATAT GTGTCTGGGA CATTTATTTC TGACTTAGAA
    AAAGACATTC CAGATCAAGA AGAAGATGAA ATTGAAGACG AATCTATAGA AATAACAAGG
    CCCCTCCGAG GTTTGGAACA CACTCCCGGT TCTAGTATGG ATAATCTCGA CAGTTCTGTG
    ACAGGCAAAA TGAACTCCTT TTCTCAGAGA CCCCGGCCTG CCAGTCCCTT TTCTACTGAC
    AGTAACACAA GTTCAGCTGT GAATCATAGT CAGAAGTCAC GGCCCACTAA GAAACACAAG
    GGAGGCAGAA TAGGTCAGCA ACCCATGCTT CGCAGAGAAG GGATTGCAGA TGATTTGCCT
    CCCCCACCTG ATCCTCCACC TTGTCAAAGA CAGCAAATCA TGCCCAGTCA ACGTGGTGGA
    GGTCCTACAG AAAGAAAAGC CAGTTCTCTT GAGAGGCACA CATCAAATAT GAATGATTTA
    AAAACCTCAT TAGACCGGCA TGGCAGAACA TCCTTAGAAC GGCAGCGACA AACACAGGAA
    TGGCTTGGCT CAACAGAAAG GCAGAACAAG CAGGCATTTG GATCTGAAGA GATACTAATG
    CCATACAGTA AACCTAGCTT CCCATCTCCA GGAGGGCACA GCTCCTCAGG AACAGTCTCT
    TCAAAAGAAT CAACTGGACC CAGAAAAGGG GAGGTTTTGA GAGGTCACCA ACGTAATGCC
    ATTGACTTTC TTGATGTAGG ATATTTGGGG CCACACAGTC AAGGACAGTT TACAGGTGAA
    TTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752466.1
    CDS542..4987
    Translation

    Target ORF information:

    RefSeq Version XM_031896606.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896606.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAACATCT GGATAATGCT ATCTTCACTA AGGCAGAGGG CAGCTTCTCC TGAATTCCTG 
    CTGATCCTTA TGCTGTTCGT CAGTGGGGTG ACCCAAAGCA AAATTAGTAG GACCAAGGGG
    AAGCACTGGA AAGGGGGATC CCGTCTCCGT CAAGAAGATT TCCCCCCACG GATAGTTGAG
    CATCCATCGG ATGTCATTGT GTCAAAGGGA GAGCCAACCA CCTTGAACTG CAAAGCGGAA
    GGGCGGCCTA CACCCACCAT AGAGTGGTAC AAAGACTTAG AAAGGGTTGA GACAGACAAG
    GATGACCCCC GCTCACATCG AATGCTACTG CCAAGTGGAT CTTTATTTTT TCTGCGCATT
    ATCCATGGGC GCAAGTTTAA GCCAGACGAA GGAAGTTACG TCTGTGTGGC AAGGAACTAT
    CTCGGGGAGG CTATGAGTCG AAATGCATCT CTGGAAGTGG CATTGCTCCG TGATGACTTC
    CGGCTCAACC CTACAGATGT TGTTGTGGCA GCTGGGGAAC CTGCAATTTT GGAGTGTCAA
    CCCCCCAGAG GACATCCGGA ACCAACTATA TACTGGAAAA AGGACAAAGT TAGAATTGAT
    GATAAAGATG AAAGAATTAG TATTCGAGGT GGAAAGCTGA TGCTCTCCAA TACAAGGAAA
    AGTGATGCAG GCATGTACAC TTGTGTTGGG ACCAACATGG TTGGAGAAAG AGACAGTGAC
    CCAGCAGAGC TAACAGTCTT TGAACGTCCC ACATTCCTCA GAAGGCCAAT TAATCAAGTT
    GTTTTAGAGG ATGAAGCTGT AGAATTTCGA TGTCAGGTCC AGGGGGACCC ACAGCCAACT
    GTCCGGTGGA AAAAGGATGA TGTAGATTTG CCAAGAGGAA GATACGACAT CAAGGATGAC
    TATACTTTGA GAATCAAGAA GGCTTCCAGC AGCGACGAAG GGACCTTTGC TTGTATTTCT
    GAAAACCGGG TTGGTAAAAT AGAAGCTTCT GCCACACTTA CAGTCAGAGC TCCTCCCCAG
    TTTGTGATCA GACCAAGGGA TCAGATTGTA GCACAAGGCC GGACAGTAAC ATTTGGATGT
    GAAGCTAAAG GAAATCCTCA ACCAGCTGTT TTTTGGCAAA AAGAAGGAAG CCAGAATTTA
    CTTTTTCCAA ATCAGCCTCT TCAGCCCAAC AGCAGGTACT CAGTGTCACC AACTGGAGAT
    TTAACCATCA CCAGCATTCA GCGCTCAGAC GCAGGCTACT ACATTTGTCA GGCACTGACT
    GTTGCCGGAA GCATTCTGGC CAAGGCCCAG CTGGAAGTGA CTGATGTTTT GACAGATAGA
    CCACCGCCAA TAATTCTACA AGGTCCAGCT AATCAAACTC TTGCCGTGGA TGGAACAGCA
    GTGCTGAAAT GTAAAACTAC TGGGGACCCA ATGCCAGTCA TAAGTTGGCT AAAAGAGGGA
    TTTAAGGGAT TCATAGGGAG AGATGCAAGG TTATCCATTC TAGACCAAGG GACATTACAA
    ATTAAAGGTT TAAAGGTTAC TGATTCTGGC ACCTATACTT GTGTGGCTAC TAGCTCTAGT
    GGTGAGACAA CATGGAGCGC TGTTCTGGAA GTGACAGAAT CTGGTGGTGC CATAATTAGC
    AAGAACTATG ATCTCAATGA CCTTCCTGGA TCTCCATCAA AACCTCAGGT TACAGATGTC
    AGTAAGAACA GTGTTACCCT ATCATGGCAA CCAGGATCAC CAGGGATTCT GCCTGTGACA
    TCCTACATCA TTGAGGCTTT CAGTCAATCT GTGAGCAATA GTTGGCAAAC TGTGGCCAAT
    CACGTGAAGA CAACGCTTTA CACTGTTAAA GGACTGCGCC CAAACACCAT TTATCTCTTT
    ATGGTGAGAG CAATCAATAC TGAAGGACTG AGTGACCCAA GTCCAATATC TGATCCAGTG
    CGTACACAAG ATATAAGTCC ACCTGTTCAA GGAGTAGATC ACCGGCAAGT ACAGAAAGAA
    CTTGGGGATG TGATTGTACG GCTCCATAAT CCTGTTGTTC TGACACCAAC AACAATCCAG
    GTCACCTGGA TGGTCGACCG TCAATCCCAG TTTATTCAGG GATACAGGGT AATGTACCGT
    CAGACTTCTG GTCTGCAGAC TCCATCTGCG TGGCAGAGCT TAGAGGTCAA AGTGCCTGCA
    GAGCGTAGCG CAGTGCTAAC CAACCTGAAA AAAGGAATTA CTTATGAGAT TAAGGTTCGA
    CCTTATTTTA ATGAATTTCA AGGAATGGAT AGCGAGTCAA AGTCAGCACG AACAACTGAA
    GAAGCCCCAA GTGCTCCTCC TCAGTCTGTT ACTGTTATGA CAGTGGGAAA TAACAACAGC
    ACCAGCATCA GCATCTCATG GGACCCCCCT CCATCTGACC ACCAGAATGG AATTATCCAG
    GACTACAGGA TCTGGTGCCT GGGTAATGAG ACACGTTTCC ACATCAACAA GACAGTGGAT
    GCTGCTATTC GTTCCGTGGT TATCGGTGGG CTATTCCCTG GAATTCAGTA CCGCGTTGAA
    GTCGCAGCCA GTACAAGCGC AGGCACTGGG GTAAAAAGTG CTCCCCAGCA CATACAGATA
    GGAAATCGGA ATGAAGTTGT GATCACAGAG AATAACAACA GCATAACAGA GCAAATAACT
    GATGTTGTGA AACAGCCGGC ATTCATCGCA GGAATTGGTG GTGCCTGCTG GGTAATACTC
    ATGGGATTTA GCATTTGGCT CTACTGGCGA AGAAAGAAGA GAAAGGGTCT CAGCAACTAC
    GCTGTTACAT TCCAGAGAGG AGATGGAGGA CTAATGAGTA ATGGAAGTCG TCCTGGACTT
    TTAAATGCGA ATGATCCTAG TTACCCTTGG TTGGCAGACT CCTGGCCAGC TTCCAGCATG
    CCGGTGAATA ACAGCAACAA TGGACCAAAC GATCTCGTTA ATTTTAGTTG TGGAGATGTG
    CTGCCAACAG TTCCAGGTCA AGGAGAAAAA ACAGCGACTA TGCTTTCTGA TGGAGCCATT
    TATAGCAGCA TTGATTTTAC TACAAAATCT AGCTATAATT GCCCAGGACA AATTGCACAA
    ACCACGCCAT ATGCTACTAC GCAGATACTA CATTCCAACA GCATTCATGA GCTGGCAGTT
    GACTTGCCTG ATCCACAGTG GAAATCTTCC ATTCAGCAGA AATCTGATCT CACTGGATTA
    GGCTATTGCC TTACTGACCA AGGCAAAGGG AGTAATGGTG GAAAAGACGG AAAAAGGAAG
    AAAAATAAAA ACAGCTCTAA AATCTCTAAA AACAATGGAT CCAGTTGGGC CAATGTTCCA
    CTTCCGCCCC CTCCCATCCA ACCACTGCCA GGCACTGAAA TAGAACATTA TGGCCTTGAA
    CACCAGGAAA CAGGCTATGA CACCAATGGA TGGTGCCCAC CTTTGCCAGT CCAGACCTAC
    TTACATCAAG GATCAGATGA TGAACTAGAA GAAGATGAAG AGAGAGTCCC TACACCCCCT
    GTACGTGGGG TGGCATCATC ACCTGCCATC TCATTTGGAC AACAGTCAAC AGCAACCCTC
    ACACCATCAC CACGTGAAGA AATGCAACCC ATGCTTCAGG CCCACCTGGA TGAATTAACA
    AGAGCTTACC AGTTTGACAT TGCAAAGCAA TCTTGGCATT TACACAGCAA TAACCAGCCC
    CCATTGGCCC CAGTTCCACC ACTTGGATAT GTGTCTGGGA CATTTATTTC TGACTTAGAA
    AAAGACATTC CAGATCAAGA AGAAGATGAA ATTGAAGACG AATCTATAGA AATAACAAGG
    CCCCTCCGAG GTTTGGAACA CACTCCCGGT TCTAGTATGG ATAATCTCGA CAGTTCTGTG
    ACAGGCAAAA TGAACTCCTT TTCTCAGAGA CCCCGGCCTG CCAGTCCCTT TTCTACTGAC
    AGTAACACAA GTTCAGCTGT GAATCATAGT CAGAAGTCAC GGCCCACTAA GAAACACAAG
    GGAGGCAGAA TAGGTCAGCA ACCCATGCTT CGCAGAGAAG GGATTGCAGA TGATTTGCCT
    CCCCCACCTG ATCCTCCACC TTGTCAAAGA CAGCAAATCA TGCCCAGTCA ACGTGGTGGA
    GGTCCTACAG AAAGAAAAGC CAGTTCTCTT GAGAGGCACA CATCAAATAT GAATGATTTA
    AAAACCTCAT TAGACCGGCA TGGCAGAACA TCCTTAGAAC GGCAGCGACA AACACAGGAA
    TGGCTTGGCT CAACAGAAAG GCAGAACAAG CAGGCATTTG GATCTGAAGA GATACTAATG
    CCATACAGTA AACCTAGCTT CCCATCTCCA GGAGGGCACA GCTCCTCAGG AACAGTCTCT
    TCAAAAGAAT CAACTGGACC CAGAAAAGGG GAGGTTTTGA GAGGTCACCA ACGTAATGCC
    ATTGACTTTC TTGATGTAGG ATATTTGGGG CCACACAGTC AAGGACAGTT TACAGGTGAA
    TTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa51970
    Clone ID Related Accession (Same CDS sequence) XM_031896593.1
    Accession Version XM_031896593.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4863bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product roundabout homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAACATCT GGATAATGCT ATCTTCACTA AGGCAGAGGG CAGCTTCTCC TGAATTCCTG 
    CTGATCCTTA TGCTGTTCGT CAGTGGGGTG ACCCAAAGCA AAATTAGTAG GACCAAGGGG
    AAGCACTGGA AAGGGGGATC CCGTCTCCGT CAAGAAGATT TCCCCCCACG GATAGTTGAG
    CATCCATCGG ATGTCATTGT GTCAAAGGGA GAGCCAACCA CCTTGAACTG CAAAGCGGAA
    GGGCGGCCTA CACCCACCAT AGAGTGGTAC AAAGACTTAG AAAGGGTTGA GACAGACAAG
    GATGACCCCC GCTCACATCG AATGCTACTG CCAAGTGGAT CTTTATTTTT TCTGCGCATT
    ATCCATGGGC GCAAGTTTAA GCCAGACGAA GGAAGTTACG TCTGTGTGGC AAGGAACTAT
    CTCGGGGAGG CTATGAGTCG AAATGCATCT CTGGAAGTGG CATTGCTCCG TGATGACTTC
    CGGCTCAACC CTACAGATGT TGTTGTGGCA GCTGGGGAAC CTGCAATTTT GGAGTGTCAA
    CCCCCCAGAG GACATCCGGA ACCAACTATA TACTGGAAAA AGGACAAAGT TAGAATTGAT
    GATAAAGATG AAAGAATTAG TATTCGAGGT GGAAAGCTGA TGCTCTCCAA TACAAGGAAA
    AGTGATGCAG GCATGTACAC TTGTGTTGGG ACCAACATGG TTGGAGAAAG AGACAGTGAC
    CCAGCAGAGC TAACAGTCTT TGAACGTCCC ACATTCCTCA GAAGGCCAAT TAATCAAGTT
    GTTTTAGAGG ATGAAGCTGT AGAATTTCGA TGTCAGGTCC AGGGGGACCC ACAGCCAACT
    GTCCGGTGGA AAAAGGATGA TGTAGATTTG CCAAGAGGAA GATACGACAT CAAGGATGAC
    TATACTTTGA GAATCAAGAA GGCTTCCAGC AGCGACGAAG GGACCTTTGC TTGTATTTCT
    GAAAACCGGG TTGGTAAAAT AGAAGCTTCT GCCACACTTA CAGTCAGAGT TCGCCCTGTT
    GCTCCTCCCC AGTTTGTGAT CAGACCAAGG GATCAGATTG TAGCACAAGG CCGGACAGTA
    ACATTTGGAT GTGAAGCTAA AGGAAATCCT CAACCAGCTG TTTTTTGGCA AAAAGAAGGA
    AGCCAGAATT TACTTTTTCC AAATCAGCCT CTTCAGCCCA ACAGCAGGTA CTCAGTGTCA
    CCAACTGGAG ATTTAACCAT CACCAGCATT CAGCGCTCAG ACGCAGGCTA CTACATTTGT
    CAGGCACTGA CTGTTGCCGG AAGCATTCTG GCCAAGGCCC AGCTGGAAGT GACTGATGTT
    TTGACAGATA GACCACCGCC AATAATTCTA CAAGGTCCAG CTAATCAAAC TCTTGCCGTG
    GATGGAACAG CAGTGCTGAA ATGTAAAACT ACTGGGGACC CAATGCCAGT CATAAGTTGG
    CTAAAAGAGG GATTTAAGGG ATTCATAGGG AGAGATGCAA GGTTATCCAT TCTAGACCAA
    GGGACATTAC AAATTAAAGG TTTAAAGGTT ACTGATTCTG GCACCTATAC TTGTGTGGCT
    ACTAGCTCTA GTGGTGAGAC AACATGGAGC GCTGTTCTGG AAGTGACAGA ATCTGGTGGT
    GCCATAATTA GCAAGAACTA TGATCTCAAT GACCTTCCTG GATCTCCATC AAAACCTCAG
    GTTACAGATG TCAGTAAGAA CAGTGTTACC CTATCATGGC AACCAGGATC ACCAGGGATT
    CTGCCTGTGA CATCCTACAT CATTGAGGCT TTCAGTCAAT CTGTGAGCAA TAGTTGGCAA
    ACTGTGGCCA ATCACGTGAA GACAACGCTT TACACTGTTA AAGGACTGCG CCCAAACACC
    ATTTATCTCT TTATGGTGAG AGCAATCAAT ACTGAAGGAC TGAGTGACCC AAGTCCAATA
    TCTGATCCAG TGCGTACACA AGATATAAGT CCACCTGTTC AAGGAGTAGA TCACCGGCAA
    GTACAGAAAG AACTTGGGGA TGTGATTGTA CGGCTCCATA ATCCTGTTGT TCTGACACCA
    ACAACAATCC AGGTCACCTG GATGGTCGAC CGTCAATCCC AGTTTATTCA GGGATACAGG
    GTAATGTACC GTCAGACTTC TGGTCTGCAG ACTCCATCTG CGTGGCAGAG CTTAGAGGTC
    AAAGTGCCTG CAGAGCGTAG CGCAGTGCTA ACCAACCTGA AAAAAGGAAT TACTTATGAG
    ATTAAGGTTC GACCTTATTT TAATGAATTT CAAGGAATGG ATAGCGAGTC AAAGTCAGCA
    CGAACAACTG AAGAAGCCCC AAGTGCTCCT CCTCAGTCTG TTACTGTTAT GACAGTGGGA
    AATAACAACA GCACCAGCAT CAGCATCTCA TGGGACCCCC CTCCATCTGA CCACCAGAAT
    GGAATTATCC AGGACTACAG GATCTGGTGC CTGGGTAATG AGACACGTTT CCACATCAAC
    AAGACAGTGG ATGCTGCTAT TCGTTCCGTG GTTATCGGTG GGCTATTCCC TGGAATTCAG
    TACCGCGTTG AAGTCGCAGC CAGTACAAGC GCAGGCACTG GGGTAAAAAG TGCTCCCCAG
    CACATACAGA TAGGAAATCG GAATGAAGTT GTGATCACAG AGAATAACAA CAGCATAACA
    GAGCAAATAA CTGATGTTGT GAAACAGCCG GCATTCATCG CAGGAATTGG TGGTGCCTGC
    TGGGTAATAC TCATGGGATT TAGCATTTGG CTCTACTGGC GAAGAAAGAA GAGAAAGGGT
    CTCAGCAACT ACGCTGTTCA GTCTTTCACC TTTACCCCTG CAGATTCCCC AGATACAACC
    ATGCACCACA AGGTTGCACT TGGCACAAAG AAGGGGAGCT GGCACAAAAC AGTTACATTC
    CAGAGAGGAG ATGGAGGACT AATGAGTAAT GGAAGTCGTC CTGGACTTTT AAATGCGAAT
    GATCCTAGTT ACCCTTGGTT GGCAGACTCC TGGCCAGCTT CCAGCATGCC GGTGAATAAC
    AGCAACAATG GACCAAACGA TCTCGTTAAT TTTAGTTGTG GAGATGTGCT GCCAACAGTT
    CCAGGTCAAG GAGAAAAAAC AGCGACTATG CTTTCTGATG GAGCCATTTA TAGCAGCATT
    GATTTTACTA CAAAATCTAG CTATAATTGC CCAGGACAAA TTGCACAAAC CACGCCATAT
    GCTACTACGC AGATACTACA TTCCAACAGC ATTCATGAGC TGGCAGTTGA CTTGCCTGAT
    CCACAGTGGA AATCTTCCAT TCAGCAGAAA TCTGATCTCA CTGGATTAGG CTATTGCCTT
    ACTGACCAAG GCAAAGGGAG TAATGCCTTA CTTTACATTC CTGACTATCG AATGGCTGAG
    GGATTGTCAA ATAGAATACC CCACAACCAG TCACAGGATT TCAGCACCAC CAGCTCTCAC
    AACAGCTCAG AGAGAAGTGG CAGCCTTTCA GGTGGAAAAG ACGGAAAAAG GAAGAAAAAT
    AAAAACAGCT CTAAAATCTC TAAAAACAAT GGATCCAGTT GGGCCAATGT TCCACTTCCG
    CCCCCTCCCA TCCAACCACT GCCAGGCACT GAAATAGAAC ATTATGGCCT TGAACACCAG
    GAAACAGGCT ATGACACCAA TGGATGGTGC CCACCTTTGC CAGTCCAGAC CTACTTACAT
    CAAGGATCAG ATGATGAACT AGAAGAAGAT GAAGAGAGAG TCCCTACACC CCCTGTACGT
    GGGGTGGCAT CATCACCTGC CATCTCATTT GGACAACAGT CAACAGCAAC CCTCACACCA
    TCACCACGTG AAGAAATGCA ACCCATGCTT CAGGCCCACC TGGATGAATT AACAAGAGCT
    TACCAGTTTG ACATTGCAAA GCAATCTTGG CATTTACACA GCAATAACCA GCCCCCATTG
    GCCCCAGTTC CACCACTTGG ATATGTGTCT GGGACATTTA TTTCTGACTT AGAAAAAGAC
    ATTCCAGATC AAGAAGAAGA TGAAATTGAA GACGAATCTA TAGAAATAAC AAGGCCCCTC
    CGAGGTTTGG AACACACTCC CGGTTCTAGT ATGGATAATC TCGACAGTTC TGTGACAGGT
    TCAATGGTAA ATGGATGGGG TTCTGCATCT GATGAGGATC GGAACTATTC TAGTCATAGA
    TCTAGTGTAG GTAGCGCAAC AGATGACTCG ATAATTGCAA GTGGCAACTT TGCACAATCA
    CTGGTTGCAG CAGCAGATAA AGCTGGTTTC TCACTAAATG GAAACAGCCT ATCTATAGCA
    GGCAAAATGA ACTCCTTTTC TCAGAGACCC CGGCCTGCCA GTCCCTTTTC TACTGACAGT
    AACACAAGTT CAGCTGTGAA TCATAGTCAG AAGTCACGGC CCACTAAGAA ACACAAGGGA
    GGCAGAATAG GTCAGCAACC CATGCTTCGC AGAGAAGGGA TTGCAGATGA TTTGCCTCCC
    CCACCTGATC CTCCACCTTG TCAAAGACAG CAAATCATGC CCAGTCAACG TGGTGGAGGT
    CCTACAGAAA GAAAAGCCAG TTCTCTTGAG AGGCACACAT CAAATATGAA TGATTTAAAA
    ACCTCATTAG ACCGGCATGG CAGAACATCC TTAGAACGGC AGCGACAAAC ACAGGAATGG
    CTTGGCTCAA CAGAAAGGCA GAACAAGCAG GCATTTGGAT CTGAAGAGAT ACTAATGCCA
    TACAGTAAAC CTAGCTTCCC ATCTCCAGGA GGGCACAGCT CCTCAGGAAC AGTCTCTTCA
    AAAGAATCAA CTGGACCCAG AAAAGGGGAG GTTTTGAGAG GTCACCAACG TAATGCCATT
    GACTTTCTTG ATGTAGGATA TTTGGGGCCA CACAGTCAAG GACAGTTTAC AGGTGAATTA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752453.1
    CDS546..5408
    Translation

    Target ORF information:

    RefSeq Version XM_031896593.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896593.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAACATCT GGATAATGCT ATCTTCACTA AGGCAGAGGG CAGCTTCTCC TGAATTCCTG 
    CTGATCCTTA TGCTGTTCGT CAGTGGGGTG ACCCAAAGCA AAATTAGTAG GACCAAGGGG
    AAGCACTGGA AAGGGGGATC CCGTCTCCGT CAAGAAGATT TCCCCCCACG GATAGTTGAG
    CATCCATCGG ATGTCATTGT GTCAAAGGGA GAGCCAACCA CCTTGAACTG CAAAGCGGAA
    GGGCGGCCTA CACCCACCAT AGAGTGGTAC AAAGACTTAG AAAGGGTTGA GACAGACAAG
    GATGACCCCC GCTCACATCG AATGCTACTG CCAAGTGGAT CTTTATTTTT TCTGCGCATT
    ATCCATGGGC GCAAGTTTAA GCCAGACGAA GGAAGTTACG TCTGTGTGGC AAGGAACTAT
    CTCGGGGAGG CTATGAGTCG AAATGCATCT CTGGAAGTGG CATTGCTCCG TGATGACTTC
    CGGCTCAACC CTACAGATGT TGTTGTGGCA GCTGGGGAAC CTGCAATTTT GGAGTGTCAA
    CCCCCCAGAG GACATCCGGA ACCAACTATA TACTGGAAAA AGGACAAAGT TAGAATTGAT
    GATAAAGATG AAAGAATTAG TATTCGAGGT GGAAAGCTGA TGCTCTCCAA TACAAGGAAA
    AGTGATGCAG GCATGTACAC TTGTGTTGGG ACCAACATGG TTGGAGAAAG AGACAGTGAC
    CCAGCAGAGC TAACAGTCTT TGAACGTCCC ACATTCCTCA GAAGGCCAAT TAATCAAGTT
    GTTTTAGAGG ATGAAGCTGT AGAATTTCGA TGTCAGGTCC AGGGGGACCC ACAGCCAACT
    GTCCGGTGGA AAAAGGATGA TGTAGATTTG CCAAGAGGAA GATACGACAT CAAGGATGAC
    TATACTTTGA GAATCAAGAA GGCTTCCAGC AGCGACGAAG GGACCTTTGC TTGTATTTCT
    GAAAACCGGG TTGGTAAAAT AGAAGCTTCT GCCACACTTA CAGTCAGAGT TCGCCCTGTT
    GCTCCTCCCC AGTTTGTGAT CAGACCAAGG GATCAGATTG TAGCACAAGG CCGGACAGTA
    ACATTTGGAT GTGAAGCTAA AGGAAATCCT CAACCAGCTG TTTTTTGGCA AAAAGAAGGA
    AGCCAGAATT TACTTTTTCC AAATCAGCCT CTTCAGCCCA ACAGCAGGTA CTCAGTGTCA
    CCAACTGGAG ATTTAACCAT CACCAGCATT CAGCGCTCAG ACGCAGGCTA CTACATTTGT
    CAGGCACTGA CTGTTGCCGG AAGCATTCTG GCCAAGGCCC AGCTGGAAGT GACTGATGTT
    TTGACAGATA GACCACCGCC AATAATTCTA CAAGGTCCAG CTAATCAAAC TCTTGCCGTG
    GATGGAACAG CAGTGCTGAA ATGTAAAACT ACTGGGGACC CAATGCCAGT CATAAGTTGG
    CTAAAAGAGG GATTTAAGGG ATTCATAGGG AGAGATGCAA GGTTATCCAT TCTAGACCAA
    GGGACATTAC AAATTAAAGG TTTAAAGGTT ACTGATTCTG GCACCTATAC TTGTGTGGCT
    ACTAGCTCTA GTGGTGAGAC AACATGGAGC GCTGTTCTGG AAGTGACAGA ATCTGGTGGT
    GCCATAATTA GCAAGAACTA TGATCTCAAT GACCTTCCTG GATCTCCATC AAAACCTCAG
    GTTACAGATG TCAGTAAGAA CAGTGTTACC CTATCATGGC AACCAGGATC ACCAGGGATT
    CTGCCTGTGA CATCCTACAT CATTGAGGCT TTCAGTCAAT CTGTGAGCAA TAGTTGGCAA
    ACTGTGGCCA ATCACGTGAA GACAACGCTT TACACTGTTA AAGGACTGCG CCCAAACACC
    ATTTATCTCT TTATGGTGAG AGCAATCAAT ACTGAAGGAC TGAGTGACCC AAGTCCAATA
    TCTGATCCAG TGCGTACACA AGATATAAGT CCACCTGTTC AAGGAGTAGA TCACCGGCAA
    GTACAGAAAG AACTTGGGGA TGTGATTGTA CGGCTCCATA ATCCTGTTGT TCTGACACCA
    ACAACAATCC AGGTCACCTG GATGGTCGAC CGTCAATCCC AGTTTATTCA GGGATACAGG
    GTAATGTACC GTCAGACTTC TGGTCTGCAG ACTCCATCTG CGTGGCAGAG CTTAGAGGTC
    AAAGTGCCTG CAGAGCGTAG CGCAGTGCTA ACCAACCTGA AAAAAGGAAT TACTTATGAG
    ATTAAGGTTC GACCTTATTT TAATGAATTT CAAGGAATGG ATAGCGAGTC AAAGTCAGCA
    CGAACAACTG AAGAAGCCCC AAGTGCTCCT CCTCAGTCTG TTACTGTTAT GACAGTGGGA
    AATAACAACA GCACCAGCAT CAGCATCTCA TGGGACCCCC CTCCATCTGA CCACCAGAAT
    GGAATTATCC AGGACTACAG GATCTGGTGC CTGGGTAATG AGACACGTTT CCACATCAAC
    AAGACAGTGG ATGCTGCTAT TCGTTCCGTG GTTATCGGTG GGCTATTCCC TGGAATTCAG
    TACCGCGTTG AAGTCGCAGC CAGTACAAGC GCAGGCACTG GGGTAAAAAG TGCTCCCCAG
    CACATACAGA TAGGAAATCG GAATGAAGTT GTGATCACAG AGAATAACAA CAGCATAACA
    GAGCAAATAA CTGATGTTGT GAAACAGCCG GCATTCATCG CAGGAATTGG TGGTGCCTGC
    TGGGTAATAC TCATGGGATT TAGCATTTGG CTCTACTGGC GAAGAAAGAA GAGAAAGGGT
    CTCAGCAACT ACGCTGTTCA GTCTTTCACC TTTACCCCTG CAGATTCCCC AGATACAACC
    ATGCACCACA AGGTTGCACT TGGCACAAAG AAGGGGAGCT GGCACAAAAC AGTTACATTC
    CAGAGAGGAG ATGGAGGACT AATGAGTAAT GGAAGTCGTC CTGGACTTTT AAATGCGAAT
    GATCCTAGTT ACCCTTGGTT GGCAGACTCC TGGCCAGCTT CCAGCATGCC GGTGAATAAC
    AGCAACAATG GACCAAACGA TCTCGTTAAT TTTAGTTGTG GAGATGTGCT GCCAACAGTT
    CCAGGTCAAG GAGAAAAAAC AGCGACTATG CTTTCTGATG GAGCCATTTA TAGCAGCATT
    GATTTTACTA CAAAATCTAG CTATAATTGC CCAGGACAAA TTGCACAAAC CACGCCATAT
    GCTACTACGC AGATACTACA TTCCAACAGC ATTCATGAGC TGGCAGTTGA CTTGCCTGAT
    CCACAGTGGA AATCTTCCAT TCAGCAGAAA TCTGATCTCA CTGGATTAGG CTATTGCCTT
    ACTGACCAAG GCAAAGGGAG TAATGCCTTA CTTTACATTC CTGACTATCG AATGGCTGAG
    GGATTGTCAA ATAGAATACC CCACAACCAG TCACAGGATT TCAGCACCAC CAGCTCTCAC
    AACAGCTCAG AGAGAAGTGG CAGCCTTTCA GGTGGAAAAG ACGGAAAAAG GAAGAAAAAT
    AAAAACAGCT CTAAAATCTC TAAAAACAAT GGATCCAGTT GGGCCAATGT TCCACTTCCG
    CCCCCTCCCA TCCAACCACT GCCAGGCACT GAAATAGAAC ATTATGGCCT TGAACACCAG
    GAAACAGGCT ATGACACCAA TGGATGGTGC CCACCTTTGC CAGTCCAGAC CTACTTACAT
    CAAGGATCAG ATGATGAACT AGAAGAAGAT GAAGAGAGAG TCCCTACACC CCCTGTACGT
    GGGGTGGCAT CATCACCTGC CATCTCATTT GGACAACAGT CAACAGCAAC CCTCACACCA
    TCACCACGTG AAGAAATGCA ACCCATGCTT CAGGCCCACC TGGATGAATT AACAAGAGCT
    TACCAGTTTG ACATTGCAAA GCAATCTTGG CATTTACACA GCAATAACCA GCCCCCATTG
    GCCCCAGTTC CACCACTTGG ATATGTGTCT GGGACATTTA TTTCTGACTT AGAAAAAGAC
    ATTCCAGATC AAGAAGAAGA TGAAATTGAA GACGAATCTA TAGAAATAAC AAGGCCCCTC
    CGAGGTTTGG AACACACTCC CGGTTCTAGT ATGGATAATC TCGACAGTTC TGTGACAGGT
    TCAATGGTAA ATGGATGGGG TTCTGCATCT GATGAGGATC GGAACTATTC TAGTCATAGA
    TCTAGTGTAG GTAGCGCAAC AGATGACTCG ATAATTGCAA GTGGCAACTT TGCACAATCA
    CTGGTTGCAG CAGCAGATAA AGCTGGTTTC TCACTAAATG GAAACAGCCT ATCTATAGCA
    GGCAAAATGA ACTCCTTTTC TCAGAGACCC CGGCCTGCCA GTCCCTTTTC TACTGACAGT
    AACACAAGTT CAGCTGTGAA TCATAGTCAG AAGTCACGGC CCACTAAGAA ACACAAGGGA
    GGCAGAATAG GTCAGCAACC CATGCTTCGC AGAGAAGGGA TTGCAGATGA TTTGCCTCCC
    CCACCTGATC CTCCACCTTG TCAAAGACAG CAAATCATGC CCAGTCAACG TGGTGGAGGT
    CCTACAGAAA GAAAAGCCAG TTCTCTTGAG AGGCACACAT CAAATATGAA TGATTTAAAA
    ACCTCATTAG ACCGGCATGG CAGAACATCC TTAGAACGGC AGCGACAAAC ACAGGAATGG
    CTTGGCTCAA CAGAAAGGCA GAACAAGCAG GCATTTGGAT CTGAAGAGAT ACTAATGCCA
    TACAGTAAAC CTAGCTTCCC ATCTCCAGGA GGGCACAGCT CCTCAGGAAC AGTCTCTTCA
    AAAGAATCAA CTGGACCCAG AAAAGGGGAG GTTTTGAGAG GTCACCAACG TAATGCCATT
    GACTTTCTTG ATGTAGGATA TTTGGGGCCA CACAGTCAAG GACAGTTTAC AGGTGAATTA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa51971
    Clone ID Related Accession (Same CDS sequence) XM_031896594.1
    Accession Version XM_031896594.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4860bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product roundabout homolog 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACATCT GGATAATGCT ATCTTCACTA AGGCAGAGGG CAGCTTCTCC TGAATTCCTG 
    CTGATCCTTA TGCTGTTCGT CAGTGGGGTG ACCCAAAGCA AAATTAGTAG GACCAAGGGG
    AAGCACTGGA AAGGGGGATC CCGTCTCCGT CAAGAAGATT TCCCCCCACG GATAGTTGAG
    CATCCATCGG ATGTCATTGT GTCAAAGGGA GAGCCAACCA CCTTGAACTG CAAAGCGGAA
    GGGCGGCCTA CACCCACCAT AGAGTGGTAC AAAGACTTAG AAAGGGTTGA GACAGACAAG
    GATGACCCCC GCTCACATCG AATGCTACTG CCAAGTGGAT CTTTATTTTT TCTGCGCATT
    ATCCATGGGC GCAAGTTTAA GCCAGACGAA GGAAGTTACG TCTGTGTGGC AAGGAACTAT
    CTCGGGGAGG CTATGAGTCG AAATGCATCT CTGGAAGTGG CATTGCTCCG TGATGACTTC
    CGGCTCAACC CTACAGATGT TGTTGTGGCA GCTGGGGAAC CTGCAATTTT GGAGTGTCAA
    CCCCCCAGAG GACATCCGGA ACCAACTATA TACTGGAAAA AGGACAAAGT TAGAATTGAT
    GATAAAGATG AAAGAATTAG TATTCGAGGT GGAAAGCTGA TGCTCTCCAA TACAAGGAAA
    AGTGATGCAG GCATGTACAC TTGTGTTGGG ACCAACATGG TTGGAGAAAG AGACAGTGAC
    CCAGCAGAGC TAACAGTCTT TGAACGTCCC ACATTCCTCA GAAGGCCAAT TAATCAAGTT
    GTTTTAGAGG ATGAAGCTGT AGAATTTCGA TGTCAGGTCC AGGGGGACCC ACAGCCAACT
    GTCCGGTGGA AAAAGGATGA TGTAGATTTG CCAAGAGGAA GATACGACAT CAAGGATGAC
    TATACTTTGA GAATCAAGAA GGCTTCCAGC AGCGACGAAG GGACCTTTGC TTGTATTTCT
    GAAAACCGGG TTGGTAAAAT AGAAGCTTCT GCCACACTTA CAGTCAGAGT TCGCCCTGTT
    GCTCCTCCCC AGTTTGTGAT CAGACCAAGG GATCAGATTG TAGCACAAGG CCGGACAGTA
    ACATTTGGAT GTGAAGCTAA AGGAAATCCT CAACCAGCTG TTTTTTGGCA AAAAGAAGGA
    AGCCAGAATT TACTTTTTCC AAATCAGCCT CTTCAGCCCA ACAGCAGGTA CTCAGTGTCA
    CCAACTGGAG ATTTAACCAT CACCAGCATT CAGCGCTCAG ACGCAGGCTA CTACATTTGT
    CAGGCACTGA CTGTTGCCGG AAGCATTCTG GCCAAGGCCC AGCTGGAAGT GACTGATGTT
    TTGACAGATA GACCACCGCC AATAATTCTA CAAGGTCCAG CTAATCAAAC TCTTGCCGTG
    GATGGAACAG CAGTGCTGAA ATGTAAAACT ACTGGGGACC CAATGCCAGT CATAAGTTGG
    CTAAAAGAGG GATTTAAGGG ATTCATAGGG AGAGATGCAA GGTTATCCAT TCTAGACCAA
    GGGACATTAC AAATTAAAGG TTTAAAGGTT ACTGATTCTG GCACCTATAC TTGTGTGGCT
    ACTAGCTCTA GTGGTGAGAC AACATGGAGC GCTGTTCTGG AAGTGACAGA ATCTGGTGGT
    GCCATAATTA GCAAGAACTA TGATCTCAAT GACCTTCCTG GATCTCCATC AAAACCTCAG
    GTTACAGATG TCAGTAAGAA CAGTGTTACC CTATCATGGC AACCAGGATC ACCAGGGATT
    CTGCCTGTGA CATCCTACAT CATTGAGGCT TTCAGTCAAT CTGTGAGCAA TAGTTGGCAA
    ACTGTGGCCA ATCACGTGAA GACAACGCTT TACACTGTTA AAGGACTGCG CCCAAACACC
    ATTTATCTCT TTATGGTGAG AGCAATCAAT ACTGAAGGAC TGAGTGACCC AAGTCCAATA
    TCTGATCCAG TGCGTACACA AGATATAAGT CCACCTGTTC AAGGAGTAGA TCACCGGCAA
    GTACAGAAAG AACTTGGGGA TGTGATTGTA CGGCTCCATA ATCCTGTTGT TCTGACACCA
    ACAACAATCC AGGTCACCTG GATGGTCGAC CGTCAATCCC AGTTTATTCA GGGATACAGG
    GTAATGTACC GTCAGACTTC TGGTCTGCAG ACTCCATCTG CGTGGCAGAG CTTAGAGGTC
    AAAGTGCCTG CAGAGCGTAG CGCAGTGCTA ACCAACCTGA AAAAAGGAAT TACTTATGAG
    ATTAAGGTTC GACCTTATTT TAATGAATTT CAAGGAATGG ATAGCGAGTC AAAGTCAGCA
    CGAACAACTG AAGAAGCCCC AAGTGCTCCT CCTCAGTCTG TTACTGTTAT GACAGTGGGA
    AATAACAACA GCACCAGCAT CAGCATCTCA TGGGACCCCC CTCCATCTGA CCACCAGAAT
    GGAATTATCC AGGACTACAG GATCTGGTGC CTGGGTAATG AGACACGTTT CCACATCAAC
    AAGACAGTGG ATGCTGCTAT TCGTTCCGTG GTTATCGGTG GGCTATTCCC TGGAATTCAG
    TACCGCGTTG AAGTCGCAGC CAGTACAAGC GCAGGCACTG GGGTAAAAAG TGCTCCCCAG
    CACATACAGA TAGGAAATCG GAATGAAGTT GTGATCACAG AGAATAACAA CAGCATAACA
    GAGCAAATAA CTGATGTTGT GAAACAGCCG GCATTCATCG CAGGAATTGG TGGTGCCTGC
    TGGGTAATAC TCATGGGATT TAGCATTTGG CTCTACTGGC GAAGAAAGAA GAGAAAGGGT
    CTCAGCAACT ACGCTGTTCA GTCTTTCACC TTTACCCCTG CAGATTCCCC AGATACAACC
    ATGCACCACA AGGTTGCACT TGGCACAAAG AAGGGGAGCT GGCACAAAAC AGTTACATTC
    CAGAGAGGAG ATGGAGGACT AATGAGTAAT GGAAGTCGTC CTGGACTTTT AAATGCGAAT
    GATCCTAGTT ACCCTTGGTT GGCAGACTCC TGGCCAGCTT CCAGCATGCC GGTGAATAAC
    AGCAACAATG GACCAAACGA TCTCGTTAAT TTTAGTTGTG GAGATGTGCT GCCAACAGTT
    CCAGGTCAAG GAGAAAAAAC AGCGACTATG CTTTCTGATG GAGCCATTTA TAGCAGCATT
    GATTTTACTA CAAAATCTAG CTATAATTGC CCAGGACAAA TTGCACAAAC CACGCCATAT
    GCTACTACGC AGATACTACA TTCCAACAGC ATTCATGAGC TGGCAGTTGA CTTGCCTGAT
    CCACAGTGGA AATCTTCCAT TCAGCAGAAA TCTGATCTCA CTGGATTAGG CTATTGCCTT
    ACTGACCAAG GCAAAGGGAG TAATGCCTTA CTTTACATTC CTGACTATCG AATGGCTGAG
    GGATTGTCAA ATAGAATACC CCACAACCAG TCACAGGATT TCAGCACCAC CAGCTCTCAC
    AACAGCTCAG AGAGAAGTGG CAGCCTTTCA GGTGGAAAAG ACGGAAAAAG GAAGAAAAAT
    AAAAACAGCT CTAAAATCTC TAAAAACAAT GGATCCAGTT GGGCCAATGT TCCACTTCCG
    CCCCCTCCCA TCCAACCACT GCCAGGCACT GAAATAGAAC ATTATGGCCT TGAACACCAG
    GAAACAGGCT ATGACACCAA TGGATGGTGC CCACCTTTGC CAGTCCAGAC CTACTTACAT
    CAAGGATCAG ATGATGAACT AGAAGAAGAT GAAGAGAGAG TCCCTACACC CCCTGTACGT
    GGGGTGGCAT CATCACCTGC CATCTCATTT GGACAACAGT CAACAGCAAC CCTCACACCA
    TCACCACGTG AAGAAATGCA ACCCATGCTT CAGGCCCACC TGGATGAATT AACAAGAGCT
    TACCAGTTTG ACATTGCAAA GCAATCTTGG CATTTACACA GCAATAACCA GCCCCCATTG
    GCCCCAGTTC CACCACTTGG ATATGTGTCT GGGACATTTA TTTCTGACTT AGAAAAAGAC
    ATTCCAGATC AAGAAGAAGA TGAAATTGAA GACGAATCTA TAGAAATAAC AAGGCCCCTC
    CGAGGTTTGG AACACACTCC CGGTTCTAGT ATGGATAATC TCGACAGTTC TGTGACAGGT
    TCAATGGTAA ATGGATGGGG TTCTGCATCT GATGAGGATC GGAACTATTC TAGTCATAGA
    TCTAGTGTAG GTAGCGCAAC AGATGACTCG ATAATTGCAA GTGGCAACTT TGCACAATCA
    CTGGTTGCAG CAGCAGATAA AGCTGGTTTC TCACTAAATG GAAACAGCCT ATCTATAGCA
    GGCAAAATGA ACTCCTTTTC TCAGAGACCC CGGCCTGCCA GTCCCTTTTC TACTGACAGT
    AACACAAGTT CAGCTGTGAA TCATAGTCAG AAGTCACGGC CCACTAAGAA ACACAAGGGA
    GGCAGAATAG GTCAGCAACC CATGCTTCGC AGAGAAGGGA TTGCAGATGA TTTGCCTCCC
    CCACCTGATC CTCCACCTTG TCAAAGACAG CAAATCATGC CCAGTCAACG TGGTGGAGGT
    CCTACAGAAA GAAAAGCCAG TTCTCTTGAG AGGCACACAT CAAATATGAA TGATTTAAAA
    ACCTCATTAG ACCGGCATGG CAGAACATCC TTAGAACGGC AGCGACAAAC ACAGGAATGG
    CTTGGCTCAA CAGAAAGGCA GAACAAGCAG GCATTTGGAT CTGAAGAGAT ACTAATGCCA
    TACAGTAAAC CTAGCTTCCC ATCTCCAGGA GGGCACAGCT CCTCAGGAAC AGTCTCTTCA
    AAAGAATCAA CTGGACCCAG AAAAGGGGAG GTTTTGAGAG GTCACCAACG TAATGCCATT
    GACTTTCTTG ATGTAGGATA TTTGGGGCCA CACAGTCAAG GACAGTTTAC AGAGTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752454.1
    CDS547..5406
    Translation

    Target ORF information:

    RefSeq Version XM_031896594.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896594.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACATCT GGATAATGCT ATCTTCACTA AGGCAGAGGG CAGCTTCTCC TGAATTCCTG 
    CTGATCCTTA TGCTGTTCGT CAGTGGGGTG ACCCAAAGCA AAATTAGTAG GACCAAGGGG
    AAGCACTGGA AAGGGGGATC CCGTCTCCGT CAAGAAGATT TCCCCCCACG GATAGTTGAG
    CATCCATCGG ATGTCATTGT GTCAAAGGGA GAGCCAACCA CCTTGAACTG CAAAGCGGAA
    GGGCGGCCTA CACCCACCAT AGAGTGGTAC AAAGACTTAG AAAGGGTTGA GACAGACAAG
    GATGACCCCC GCTCACATCG AATGCTACTG CCAAGTGGAT CTTTATTTTT TCTGCGCATT
    ATCCATGGGC GCAAGTTTAA GCCAGACGAA GGAAGTTACG TCTGTGTGGC AAGGAACTAT
    CTCGGGGAGG CTATGAGTCG AAATGCATCT CTGGAAGTGG CATTGCTCCG TGATGACTTC
    CGGCTCAACC CTACAGATGT TGTTGTGGCA GCTGGGGAAC CTGCAATTTT GGAGTGTCAA
    CCCCCCAGAG GACATCCGGA ACCAACTATA TACTGGAAAA AGGACAAAGT TAGAATTGAT
    GATAAAGATG AAAGAATTAG TATTCGAGGT GGAAAGCTGA TGCTCTCCAA TACAAGGAAA
    AGTGATGCAG GCATGTACAC TTGTGTTGGG ACCAACATGG TTGGAGAAAG AGACAGTGAC
    CCAGCAGAGC TAACAGTCTT TGAACGTCCC ACATTCCTCA GAAGGCCAAT TAATCAAGTT
    GTTTTAGAGG ATGAAGCTGT AGAATTTCGA TGTCAGGTCC AGGGGGACCC ACAGCCAACT
    GTCCGGTGGA AAAAGGATGA TGTAGATTTG CCAAGAGGAA GATACGACAT CAAGGATGAC
    TATACTTTGA GAATCAAGAA GGCTTCCAGC AGCGACGAAG GGACCTTTGC TTGTATTTCT
    GAAAACCGGG TTGGTAAAAT AGAAGCTTCT GCCACACTTA CAGTCAGAGT TCGCCCTGTT
    GCTCCTCCCC AGTTTGTGAT CAGACCAAGG GATCAGATTG TAGCACAAGG CCGGACAGTA
    ACATTTGGAT GTGAAGCTAA AGGAAATCCT CAACCAGCTG TTTTTTGGCA AAAAGAAGGA
    AGCCAGAATT TACTTTTTCC AAATCAGCCT CTTCAGCCCA ACAGCAGGTA CTCAGTGTCA
    CCAACTGGAG ATTTAACCAT CACCAGCATT CAGCGCTCAG ACGCAGGCTA CTACATTTGT
    CAGGCACTGA CTGTTGCCGG AAGCATTCTG GCCAAGGCCC AGCTGGAAGT GACTGATGTT
    TTGACAGATA GACCACCGCC AATAATTCTA CAAGGTCCAG CTAATCAAAC TCTTGCCGTG
    GATGGAACAG CAGTGCTGAA ATGTAAAACT ACTGGGGACC CAATGCCAGT CATAAGTTGG
    CTAAAAGAGG GATTTAAGGG ATTCATAGGG AGAGATGCAA GGTTATCCAT TCTAGACCAA
    GGGACATTAC AAATTAAAGG TTTAAAGGTT ACTGATTCTG GCACCTATAC TTGTGTGGCT
    ACTAGCTCTA GTGGTGAGAC AACATGGAGC GCTGTTCTGG AAGTGACAGA ATCTGGTGGT
    GCCATAATTA GCAAGAACTA TGATCTCAAT GACCTTCCTG GATCTCCATC AAAACCTCAG
    GTTACAGATG TCAGTAAGAA CAGTGTTACC CTATCATGGC AACCAGGATC ACCAGGGATT
    CTGCCTGTGA CATCCTACAT CATTGAGGCT TTCAGTCAAT CTGTGAGCAA TAGTTGGCAA
    ACTGTGGCCA ATCACGTGAA GACAACGCTT TACACTGTTA AAGGACTGCG CCCAAACACC
    ATTTATCTCT TTATGGTGAG AGCAATCAAT ACTGAAGGAC TGAGTGACCC AAGTCCAATA
    TCTGATCCAG TGCGTACACA AGATATAAGT CCACCTGTTC AAGGAGTAGA TCACCGGCAA
    GTACAGAAAG AACTTGGGGA TGTGATTGTA CGGCTCCATA ATCCTGTTGT TCTGACACCA
    ACAACAATCC AGGTCACCTG GATGGTCGAC CGTCAATCCC AGTTTATTCA GGGATACAGG
    GTAATGTACC GTCAGACTTC TGGTCTGCAG ACTCCATCTG CGTGGCAGAG CTTAGAGGTC
    AAAGTGCCTG CAGAGCGTAG CGCAGTGCTA ACCAACCTGA AAAAAGGAAT TACTTATGAG
    ATTAAGGTTC GACCTTATTT TAATGAATTT CAAGGAATGG ATAGCGAGTC AAAGTCAGCA
    CGAACAACTG AAGAAGCCCC AAGTGCTCCT CCTCAGTCTG TTACTGTTAT GACAGTGGGA
    AATAACAACA GCACCAGCAT CAGCATCTCA TGGGACCCCC CTCCATCTGA CCACCAGAAT
    GGAATTATCC AGGACTACAG GATCTGGTGC CTGGGTAATG AGACACGTTT CCACATCAAC
    AAGACAGTGG ATGCTGCTAT TCGTTCCGTG GTTATCGGTG GGCTATTCCC TGGAATTCAG
    TACCGCGTTG AAGTCGCAGC CAGTACAAGC GCAGGCACTG GGGTAAAAAG TGCTCCCCAG
    CACATACAGA TAGGAAATCG GAATGAAGTT GTGATCACAG AGAATAACAA CAGCATAACA
    GAGCAAATAA CTGATGTTGT GAAACAGCCG GCATTCATCG CAGGAATTGG TGGTGCCTGC
    TGGGTAATAC TCATGGGATT TAGCATTTGG CTCTACTGGC GAAGAAAGAA GAGAAAGGGT
    CTCAGCAACT ACGCTGTTCA GTCTTTCACC TTTACCCCTG CAGATTCCCC AGATACAACC
    ATGCACCACA AGGTTGCACT TGGCACAAAG AAGGGGAGCT GGCACAAAAC AGTTACATTC
    CAGAGAGGAG ATGGAGGACT AATGAGTAAT GGAAGTCGTC CTGGACTTTT AAATGCGAAT
    GATCCTAGTT ACCCTTGGTT GGCAGACTCC TGGCCAGCTT CCAGCATGCC GGTGAATAAC
    AGCAACAATG GACCAAACGA TCTCGTTAAT TTTAGTTGTG GAGATGTGCT GCCAACAGTT
    CCAGGTCAAG GAGAAAAAAC AGCGACTATG CTTTCTGATG GAGCCATTTA TAGCAGCATT
    GATTTTACTA CAAAATCTAG CTATAATTGC CCAGGACAAA TTGCACAAAC CACGCCATAT
    GCTACTACGC AGATACTACA TTCCAACAGC ATTCATGAGC TGGCAGTTGA CTTGCCTGAT
    CCACAGTGGA AATCTTCCAT TCAGCAGAAA TCTGATCTCA CTGGATTAGG CTATTGCCTT
    ACTGACCAAG GCAAAGGGAG TAATGCCTTA CTTTACATTC CTGACTATCG AATGGCTGAG
    GGATTGTCAA ATAGAATACC CCACAACCAG TCACAGGATT TCAGCACCAC CAGCTCTCAC
    AACAGCTCAG AGAGAAGTGG CAGCCTTTCA GGTGGAAAAG ACGGAAAAAG GAAGAAAAAT
    AAAAACAGCT CTAAAATCTC TAAAAACAAT GGATCCAGTT GGGCCAATGT TCCACTTCCG
    CCCCCTCCCA TCCAACCACT GCCAGGCACT GAAATAGAAC ATTATGGCCT TGAACACCAG
    GAAACAGGCT ATGACACCAA TGGATGGTGC CCACCTTTGC CAGTCCAGAC CTACTTACAT
    CAAGGATCAG ATGATGAACT AGAAGAAGAT GAAGAGAGAG TCCCTACACC CCCTGTACGT
    GGGGTGGCAT CATCACCTGC CATCTCATTT GGACAACAGT CAACAGCAAC CCTCACACCA
    TCACCACGTG AAGAAATGCA ACCCATGCTT CAGGCCCACC TGGATGAATT AACAAGAGCT
    TACCAGTTTG ACATTGCAAA GCAATCTTGG CATTTACACA GCAATAACCA GCCCCCATTG
    GCCCCAGTTC CACCACTTGG ATATGTGTCT GGGACATTTA TTTCTGACTT AGAAAAAGAC
    ATTCCAGATC AAGAAGAAGA TGAAATTGAA GACGAATCTA TAGAAATAAC AAGGCCCCTC
    CGAGGTTTGG AACACACTCC CGGTTCTAGT ATGGATAATC TCGACAGTTC TGTGACAGGT
    TCAATGGTAA ATGGATGGGG TTCTGCATCT GATGAGGATC GGAACTATTC TAGTCATAGA
    TCTAGTGTAG GTAGCGCAAC AGATGACTCG ATAATTGCAA GTGGCAACTT TGCACAATCA
    CTGGTTGCAG CAGCAGATAA AGCTGGTTTC TCACTAAATG GAAACAGCCT ATCTATAGCA
    GGCAAAATGA ACTCCTTTTC TCAGAGACCC CGGCCTGCCA GTCCCTTTTC TACTGACAGT
    AACACAAGTT CAGCTGTGAA TCATAGTCAG AAGTCACGGC CCACTAAGAA ACACAAGGGA
    GGCAGAATAG GTCAGCAACC CATGCTTCGC AGAGAAGGGA TTGCAGATGA TTTGCCTCCC
    CCACCTGATC CTCCACCTTG TCAAAGACAG CAAATCATGC CCAGTCAACG TGGTGGAGGT
    CCTACAGAAA GAAAAGCCAG TTCTCTTGAG AGGCACACAT CAAATATGAA TGATTTAAAA
    ACCTCATTAG ACCGGCATGG CAGAACATCC TTAGAACGGC AGCGACAAAC ACAGGAATGG
    CTTGGCTCAA CAGAAAGGCA GAACAAGCAG GCATTTGGAT CTGAAGAGAT ACTAATGCCA
    TACAGTAAAC CTAGCTTCCC ATCTCCAGGA GGGCACAGCT CCTCAGGAAC AGTCTCTTCA
    AAAGAATCAA CTGGACCCAG AAAAGGGGAG GTTTTGAGAG GTCACCAACG TAATGCCATT
    GACTTTCTTG ATGTAGGATA TTTGGGGCCA CACAGTCAAG GACAGTTTAC AGAGTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa51972
    Clone ID Related Accession (Same CDS sequence) XM_031896595.1
    Accession Version XM_031896595.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4851bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product roundabout homolog 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACATCT GGATAATGCT ATCTTCACTA AGGCAGAGGG CAGCTTCTCC TGAATTCCTG 
    CTGATCCTTA TGCTGTTCGT CAGTGGGGTG ACCCAAAGCA AAATTAGTAG GACCAAGGGG
    AAGCACTGGA AAGGGGGATC CCGTCTCCGT CAAGAAGATT TCCCCCCACG GATAGTTGAG
    CATCCATCGG ATGTCATTGT GTCAAAGGGA GAGCCAACCA CCTTGAACTG CAAAGCGGAA
    GGGCGGCCTA CACCCACCAT AGAGTGGTAC AAAGACTTAG AAAGGGTTGA GACAGACAAG
    GATGACCCCC GCTCACATCG AATGCTACTG CCAAGTGGAT CTTTATTTTT TCTGCGCATT
    ATCCATGGGC GCAAGTTTAA GCCAGACGAA GGAAGTTACG TCTGTGTGGC AAGGAACTAT
    CTCGGGGAGG CTATGAGTCG AAATGCATCT CTGGAAGTGG CATTGCTCCG TGATGACTTC
    CGGCTCAACC CTACAGATGT TGTTGTGGCA GCTGGGGAAC CTGCAATTTT GGAGTGTCAA
    CCCCCCAGAG GACATCCGGA ACCAACTATA TACTGGAAAA AGGACAAAGT TAGAATTGAT
    GATAAAGATG AAAGAATTAG TATTCGAGGT GGAAAGCTGA TGCTCTCCAA TACAAGGAAA
    AGTGATGCAG GCATGTACAC TTGTGTTGGG ACCAACATGG TTGGAGAAAG AGACAGTGAC
    CCAGCAGAGC TAACAGTCTT TGAACGTCCC ACATTCCTCA GAAGGCCAAT TAATCAAGTT
    GTTTTAGAGG ATGAAGCTGT AGAATTTCGA TGTCAGGTCC AGGGGGACCC ACAGCCAACT
    GTCCGGTGGA AAAAGGATGA TGTAGATTTG CCAAGAGGAA GATACGACAT CAAGGATGAC
    TATACTTTGA GAATCAAGAA GGCTTCCAGC AGCGACGAAG GGACCTTTGC TTGTATTTCT
    GAAAACCGGG TTGGTAAAAT AGAAGCTTCT GCCACACTTA CAGTCAGAGC TCCTCCCCAG
    TTTGTGATCA GACCAAGGGA TCAGATTGTA GCACAAGGCC GGACAGTAAC ATTTGGATGT
    GAAGCTAAAG GAAATCCTCA ACCAGCTGTT TTTTGGCAAA AAGAAGGAAG CCAGAATTTA
    CTTTTTCCAA ATCAGCCTCT TCAGCCCAAC AGCAGGTACT CAGTGTCACC AACTGGAGAT
    TTAACCATCA CCAGCATTCA GCGCTCAGAC GCAGGCTACT ACATTTGTCA GGCACTGACT
    GTTGCCGGAA GCATTCTGGC CAAGGCCCAG CTGGAAGTGA CTGATGTTTT GACAGATAGA
    CCACCGCCAA TAATTCTACA AGGTCCAGCT AATCAAACTC TTGCCGTGGA TGGAACAGCA
    GTGCTGAAAT GTAAAACTAC TGGGGACCCA ATGCCAGTCA TAAGTTGGCT AAAAGAGGGA
    TTTAAGGGAT TCATAGGGAG AGATGCAAGG TTATCCATTC TAGACCAAGG GACATTACAA
    ATTAAAGGTT TAAAGGTTAC TGATTCTGGC ACCTATACTT GTGTGGCTAC TAGCTCTAGT
    GGTGAGACAA CATGGAGCGC TGTTCTGGAA GTGACAGAAT CTGGTGGTGC CATAATTAGC
    AAGAACTATG ATCTCAATGA CCTTCCTGGA TCTCCATCAA AACCTCAGGT TACAGATGTC
    AGTAAGAACA GTGTTACCCT ATCATGGCAA CCAGGATCAC CAGGGATTCT GCCTGTGACA
    TCCTACATCA TTGAGGCTTT CAGTCAATCT GTGAGCAATA GTTGGCAAAC TGTGGCCAAT
    CACGTGAAGA CAACGCTTTA CACTGTTAAA GGACTGCGCC CAAACACCAT TTATCTCTTT
    ATGGTGAGAG CAATCAATAC TGAAGGACTG AGTGACCCAA GTCCAATATC TGATCCAGTG
    CGTACACAAG ATATAAGTCC ACCTGTTCAA GGAGTAGATC ACCGGCAAGT ACAGAAAGAA
    CTTGGGGATG TGATTGTACG GCTCCATAAT CCTGTTGTTC TGACACCAAC AACAATCCAG
    GTCACCTGGA TGGTCGACCG TCAATCCCAG TTTATTCAGG GATACAGGGT AATGTACCGT
    CAGACTTCTG GTCTGCAGAC TCCATCTGCG TGGCAGAGCT TAGAGGTCAA AGTGCCTGCA
    GAGCGTAGCG CAGTGCTAAC CAACCTGAAA AAAGGAATTA CTTATGAGAT TAAGGTTCGA
    CCTTATTTTA ATGAATTTCA AGGAATGGAT AGCGAGTCAA AGTCAGCACG AACAACTGAA
    GAAGCCCCAA GTGCTCCTCC TCAGTCTGTT ACTGTTATGA CAGTGGGAAA TAACAACAGC
    ACCAGCATCA GCATCTCATG GGACCCCCCT CCATCTGACC ACCAGAATGG AATTATCCAG
    GACTACAGGA TCTGGTGCCT GGGTAATGAG ACACGTTTCC ACATCAACAA GACAGTGGAT
    GCTGCTATTC GTTCCGTGGT TATCGGTGGG CTATTCCCTG GAATTCAGTA CCGCGTTGAA
    GTCGCAGCCA GTACAAGCGC AGGCACTGGG GTAAAAAGTG CTCCCCAGCA CATACAGATA
    GGAAATCGGA ATGAAGTTGT GATCACAGAG AATAACAACA GCATAACAGA GCAAATAACT
    GATGTTGTGA AACAGCCGGC ATTCATCGCA GGAATTGGTG GTGCCTGCTG GGTAATACTC
    ATGGGATTTA GCATTTGGCT CTACTGGCGA AGAAAGAAGA GAAAGGGTCT CAGCAACTAC
    GCTGTTCAGT CTTTCACCTT TACCCCTGCA GATTCCCCAG ATACAACCAT GCACCACAAG
    GTTGCACTTG GCACAAAGAA GGGGAGCTGG CACAAAACAG TTACATTCCA GAGAGGAGAT
    GGAGGACTAA TGAGTAATGG AAGTCGTCCT GGACTTTTAA ATGCGAATGA TCCTAGTTAC
    CCTTGGTTGG CAGACTCCTG GCCAGCTTCC AGCATGCCGG TGAATAACAG CAACAATGGA
    CCAAACGATC TCGTTAATTT TAGTTGTGGA GATGTGCTGC CAACAGTTCC AGGTCAAGGA
    GAAAAAACAG CGACTATGCT TTCTGATGGA GCCATTTATA GCAGCATTGA TTTTACTACA
    AAATCTAGCT ATAATTGCCC AGGACAAATT GCACAAACCA CGCCATATGC TACTACGCAG
    ATACTACATT CCAACAGCAT TCATGAGCTG GCAGTTGACT TGCCTGATCC ACAGTGGAAA
    TCTTCCATTC AGCAGAAATC TGATCTCACT GGATTAGGCT ATTGCCTTAC TGACCAAGGC
    AAAGGGAGTA ATGCCTTACT TTACATTCCT GACTATCGAA TGGCTGAGGG ATTGTCAAAT
    AGAATACCCC ACAACCAGTC ACAGGATTTC AGCACCACCA GCTCTCACAA CAGCTCAGAG
    AGAAGTGGCA GCCTTTCAGG TGGAAAAGAC GGAAAAAGGA AGAAAAATAA AAACAGCTCT
    AAAATCTCTA AAAACAATGG ATCCAGTTGG GCCAATGTTC CACTTCCGCC CCCTCCCATC
    CAACCACTGC CAGGCACTGA AATAGAACAT TATGGCCTTG AACACCAGGA AACAGGCTAT
    GACACCAATG GATGGTGCCC ACCTTTGCCA GTCCAGACCT ACTTACATCA AGGATCAGAT
    GATGAACTAG AAGAAGATGA AGAGAGAGTC CCTACACCCC CTGTACGTGG GGTGGCATCA
    TCACCTGCCA TCTCATTTGG ACAACAGTCA ACAGCAACCC TCACACCATC ACCACGTGAA
    GAAATGCAAC CCATGCTTCA GGCCCACCTG GATGAATTAA CAAGAGCTTA CCAGTTTGAC
    ATTGCAAAGC AATCTTGGCA TTTACACAGC AATAACCAGC CCCCATTGGC CCCAGTTCCA
    CCACTTGGAT ATGTGTCTGG GACATTTATT TCTGACTTAG AAAAAGACAT TCCAGATCAA
    GAAGAAGATG AAATTGAAGA CGAATCTATA GAAATAACAA GGCCCCTCCG AGGTTTGGAA
    CACACTCCCG GTTCTAGTAT GGATAATCTC GACAGTTCTG TGACAGGTTC AATGGTAAAT
    GGATGGGGTT CTGCATCTGA TGAGGATCGG AACTATTCTA GTCATAGATC TAGTGTAGGT
    AGCGCAACAG ATGACTCGAT AATTGCAAGT GGCAACTTTG CACAATCACT GGTTGCAGCA
    GCAGATAAAG CTGGTTTCTC ACTAAATGGA AACAGCCTAT CTATAGCAGG CAAAATGAAC
    TCCTTTTCTC AGAGACCCCG GCCTGCCAGT CCCTTTTCTA CTGACAGTAA CACAAGTTCA
    GCTGTGAATC ATAGTCAGAA GTCACGGCCC ACTAAGAAAC ACAAGGGAGG CAGAATAGGT
    CAGCAACCCA TGCTTCGCAG AGAAGGGATT GCAGATGATT TGCCTCCCCC ACCTGATCCT
    CCACCTTGTC AAAGACAGCA AATCATGCCC AGTCAACGTG GTGGAGGTCC TACAGAAAGA
    AAAGCCAGTT CTCTTGAGAG GCACACATCA AATATGAATG ATTTAAAAAC CTCATTAGAC
    CGGCATGGCA GAACATCCTT AGAACGGCAG CGACAAACAC AGGAATGGCT TGGCTCAACA
    GAAAGGCAGA ACAAGCAGGC ATTTGGATCT GAAGAGATAC TAATGCCATA CAGTAAACCT
    AGCTTCCCAT CTCCAGGAGG GCACAGCTCC TCAGGAACAG TCTCTTCAAA AGAATCAACT
    GGACCCAGAA AAGGGGAGGT TTTGAGAGGT CACCAACGTA ATGCCATTGA CTTTCTTGAT
    GTAGGATATT TGGGGCCACA CAGTCAAGGA CAGTTTACAG GTGAATTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752455.1
    CDS546..5396
    Translation

    Target ORF information:

    RefSeq Version XM_031896595.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896595.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACATCT GGATAATGCT ATCTTCACTA AGGCAGAGGG CAGCTTCTCC TGAATTCCTG 
    CTGATCCTTA TGCTGTTCGT CAGTGGGGTG ACCCAAAGCA AAATTAGTAG GACCAAGGGG
    AAGCACTGGA AAGGGGGATC CCGTCTCCGT CAAGAAGATT TCCCCCCACG GATAGTTGAG
    CATCCATCGG ATGTCATTGT GTCAAAGGGA GAGCCAACCA CCTTGAACTG CAAAGCGGAA
    GGGCGGCCTA CACCCACCAT AGAGTGGTAC AAAGACTTAG AAAGGGTTGA GACAGACAAG
    GATGACCCCC GCTCACATCG AATGCTACTG CCAAGTGGAT CTTTATTTTT TCTGCGCATT
    ATCCATGGGC GCAAGTTTAA GCCAGACGAA GGAAGTTACG TCTGTGTGGC AAGGAACTAT
    CTCGGGGAGG CTATGAGTCG AAATGCATCT CTGGAAGTGG CATTGCTCCG TGATGACTTC
    CGGCTCAACC CTACAGATGT TGTTGTGGCA GCTGGGGAAC CTGCAATTTT GGAGTGTCAA
    CCCCCCAGAG GACATCCGGA ACCAACTATA TACTGGAAAA AGGACAAAGT TAGAATTGAT
    GATAAAGATG AAAGAATTAG TATTCGAGGT GGAAAGCTGA TGCTCTCCAA TACAAGGAAA
    AGTGATGCAG GCATGTACAC TTGTGTTGGG ACCAACATGG TTGGAGAAAG AGACAGTGAC
    CCAGCAGAGC TAACAGTCTT TGAACGTCCC ACATTCCTCA GAAGGCCAAT TAATCAAGTT
    GTTTTAGAGG ATGAAGCTGT AGAATTTCGA TGTCAGGTCC AGGGGGACCC ACAGCCAACT
    GTCCGGTGGA AAAAGGATGA TGTAGATTTG CCAAGAGGAA GATACGACAT CAAGGATGAC
    TATACTTTGA GAATCAAGAA GGCTTCCAGC AGCGACGAAG GGACCTTTGC TTGTATTTCT
    GAAAACCGGG TTGGTAAAAT AGAAGCTTCT GCCACACTTA CAGTCAGAGC TCCTCCCCAG
    TTTGTGATCA GACCAAGGGA TCAGATTGTA GCACAAGGCC GGACAGTAAC ATTTGGATGT
    GAAGCTAAAG GAAATCCTCA ACCAGCTGTT TTTTGGCAAA AAGAAGGAAG CCAGAATTTA
    CTTTTTCCAA ATCAGCCTCT TCAGCCCAAC AGCAGGTACT CAGTGTCACC AACTGGAGAT
    TTAACCATCA CCAGCATTCA GCGCTCAGAC GCAGGCTACT ACATTTGTCA GGCACTGACT
    GTTGCCGGAA GCATTCTGGC CAAGGCCCAG CTGGAAGTGA CTGATGTTTT GACAGATAGA
    CCACCGCCAA TAATTCTACA AGGTCCAGCT AATCAAACTC TTGCCGTGGA TGGAACAGCA
    GTGCTGAAAT GTAAAACTAC TGGGGACCCA ATGCCAGTCA TAAGTTGGCT AAAAGAGGGA
    TTTAAGGGAT TCATAGGGAG AGATGCAAGG TTATCCATTC TAGACCAAGG GACATTACAA
    ATTAAAGGTT TAAAGGTTAC TGATTCTGGC ACCTATACTT GTGTGGCTAC TAGCTCTAGT
    GGTGAGACAA CATGGAGCGC TGTTCTGGAA GTGACAGAAT CTGGTGGTGC CATAATTAGC
    AAGAACTATG ATCTCAATGA CCTTCCTGGA TCTCCATCAA AACCTCAGGT TACAGATGTC
    AGTAAGAACA GTGTTACCCT ATCATGGCAA CCAGGATCAC CAGGGATTCT GCCTGTGACA
    TCCTACATCA TTGAGGCTTT CAGTCAATCT GTGAGCAATA GTTGGCAAAC TGTGGCCAAT
    CACGTGAAGA CAACGCTTTA CACTGTTAAA GGACTGCGCC CAAACACCAT TTATCTCTTT
    ATGGTGAGAG CAATCAATAC TGAAGGACTG AGTGACCCAA GTCCAATATC TGATCCAGTG
    CGTACACAAG ATATAAGTCC ACCTGTTCAA GGAGTAGATC ACCGGCAAGT ACAGAAAGAA
    CTTGGGGATG TGATTGTACG GCTCCATAAT CCTGTTGTTC TGACACCAAC AACAATCCAG
    GTCACCTGGA TGGTCGACCG TCAATCCCAG TTTATTCAGG GATACAGGGT AATGTACCGT
    CAGACTTCTG GTCTGCAGAC TCCATCTGCG TGGCAGAGCT TAGAGGTCAA AGTGCCTGCA
    GAGCGTAGCG CAGTGCTAAC CAACCTGAAA AAAGGAATTA CTTATGAGAT TAAGGTTCGA
    CCTTATTTTA ATGAATTTCA AGGAATGGAT AGCGAGTCAA AGTCAGCACG AACAACTGAA
    GAAGCCCCAA GTGCTCCTCC TCAGTCTGTT ACTGTTATGA CAGTGGGAAA TAACAACAGC
    ACCAGCATCA GCATCTCATG GGACCCCCCT CCATCTGACC ACCAGAATGG AATTATCCAG
    GACTACAGGA TCTGGTGCCT GGGTAATGAG ACACGTTTCC ACATCAACAA GACAGTGGAT
    GCTGCTATTC GTTCCGTGGT TATCGGTGGG CTATTCCCTG GAATTCAGTA CCGCGTTGAA
    GTCGCAGCCA GTACAAGCGC AGGCACTGGG GTAAAAAGTG CTCCCCAGCA CATACAGATA
    GGAAATCGGA ATGAAGTTGT GATCACAGAG AATAACAACA GCATAACAGA GCAAATAACT
    GATGTTGTGA AACAGCCGGC ATTCATCGCA GGAATTGGTG GTGCCTGCTG GGTAATACTC
    ATGGGATTTA GCATTTGGCT CTACTGGCGA AGAAAGAAGA GAAAGGGTCT CAGCAACTAC
    GCTGTTCAGT CTTTCACCTT TACCCCTGCA GATTCCCCAG ATACAACCAT GCACCACAAG
    GTTGCACTTG GCACAAAGAA GGGGAGCTGG CACAAAACAG TTACATTCCA GAGAGGAGAT
    GGAGGACTAA TGAGTAATGG AAGTCGTCCT GGACTTTTAA ATGCGAATGA TCCTAGTTAC
    CCTTGGTTGG CAGACTCCTG GCCAGCTTCC AGCATGCCGG TGAATAACAG CAACAATGGA
    CCAAACGATC TCGTTAATTT TAGTTGTGGA GATGTGCTGC CAACAGTTCC AGGTCAAGGA
    GAAAAAACAG CGACTATGCT TTCTGATGGA GCCATTTATA GCAGCATTGA TTTTACTACA
    AAATCTAGCT ATAATTGCCC AGGACAAATT GCACAAACCA CGCCATATGC TACTACGCAG
    ATACTACATT CCAACAGCAT TCATGAGCTG GCAGTTGACT TGCCTGATCC ACAGTGGAAA
    TCTTCCATTC AGCAGAAATC TGATCTCACT GGATTAGGCT ATTGCCTTAC TGACCAAGGC
    AAAGGGAGTA ATGCCTTACT TTACATTCCT GACTATCGAA TGGCTGAGGG ATTGTCAAAT
    AGAATACCCC ACAACCAGTC ACAGGATTTC AGCACCACCA GCTCTCACAA CAGCTCAGAG
    AGAAGTGGCA GCCTTTCAGG TGGAAAAGAC GGAAAAAGGA AGAAAAATAA AAACAGCTCT
    AAAATCTCTA AAAACAATGG ATCCAGTTGG GCCAATGTTC CACTTCCGCC CCCTCCCATC
    CAACCACTGC CAGGCACTGA AATAGAACAT TATGGCCTTG AACACCAGGA AACAGGCTAT
    GACACCAATG GATGGTGCCC ACCTTTGCCA GTCCAGACCT ACTTACATCA AGGATCAGAT
    GATGAACTAG AAGAAGATGA AGAGAGAGTC CCTACACCCC CTGTACGTGG GGTGGCATCA
    TCACCTGCCA TCTCATTTGG ACAACAGTCA ACAGCAACCC TCACACCATC ACCACGTGAA
    GAAATGCAAC CCATGCTTCA GGCCCACCTG GATGAATTAA CAAGAGCTTA CCAGTTTGAC
    ATTGCAAAGC AATCTTGGCA TTTACACAGC AATAACCAGC CCCCATTGGC CCCAGTTCCA
    CCACTTGGAT ATGTGTCTGG GACATTTATT TCTGACTTAG AAAAAGACAT TCCAGATCAA
    GAAGAAGATG AAATTGAAGA CGAATCTATA GAAATAACAA GGCCCCTCCG AGGTTTGGAA
    CACACTCCCG GTTCTAGTAT GGATAATCTC GACAGTTCTG TGACAGGTTC AATGGTAAAT
    GGATGGGGTT CTGCATCTGA TGAGGATCGG AACTATTCTA GTCATAGATC TAGTGTAGGT
    AGCGCAACAG ATGACTCGAT AATTGCAAGT GGCAACTTTG CACAATCACT GGTTGCAGCA
    GCAGATAAAG CTGGTTTCTC ACTAAATGGA AACAGCCTAT CTATAGCAGG CAAAATGAAC
    TCCTTTTCTC AGAGACCCCG GCCTGCCAGT CCCTTTTCTA CTGACAGTAA CACAAGTTCA
    GCTGTGAATC ATAGTCAGAA GTCACGGCCC ACTAAGAAAC ACAAGGGAGG CAGAATAGGT
    CAGCAACCCA TGCTTCGCAG AGAAGGGATT GCAGATGATT TGCCTCCCCC ACCTGATCCT
    CCACCTTGTC AAAGACAGCA AATCATGCCC AGTCAACGTG GTGGAGGTCC TACAGAAAGA
    AAAGCCAGTT CTCTTGAGAG GCACACATCA AATATGAATG ATTTAAAAAC CTCATTAGAC
    CGGCATGGCA GAACATCCTT AGAACGGCAG CGACAAACAC AGGAATGGCT TGGCTCAACA
    GAAAGGCAGA ACAAGCAGGC ATTTGGATCT GAAGAGATAC TAATGCCATA CAGTAAACCT
    AGCTTCCCAT CTCCAGGAGG GCACAGCTCC TCAGGAACAG TCTCTTCAAA AGAATCAACT
    GGACCCAGAA AAGGGGAGGT TTTGAGAGGT CACCAACGTA ATGCCATTGA CTTTCTTGAT
    GTAGGATATT TGGGGCCACA CAGTCAAGGA CAGTTTACAG GTGAATTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa51973
    Clone ID Related Accession (Same CDS sequence) XM_031896596.1
    Accession Version XM_031896596.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4794bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product roundabout homolog 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAACATCT GGATAATGCT ATCTTCACTA AGGCAGAGGG CAGCTTCTCC TGAATTCCTG 
    CTGATCCTTA TGCTGTTCGT CAGTGGGGTG ACCCAAAGCA AAATTAGTAG GACCAAGGGG
    AAGCACTGGA AAGGGGGATC CCGTCTCCGT CAAGAAGATT TCCCCCCACG GATAGTTGAG
    CATCCATCGG ATGTCATTGT GTCAAAGGGA GAGCCAACCA CCTTGAACTG CAAAGCGGAA
    GGGCGGCCTA CACCCACCAT AGAGTGGTAC AAAGACTTAG AAAGGGTTGA GACAGACAAG
    GATGACCCCC GCTCACATCG AATGCTACTG CCAAGTGGAT CTTTATTTTT TCTGCGCATT
    ATCCATGGGC GCAAGTTTAA GCCAGACGAA GGAAGTTACG TCTGTGTGGC AAGGAACTAT
    CTCGGGGAGG CTATGAGTCG AAATGCATCT CTGGAAGTGG CATTGCTCCG TGATGACTTC
    CGGCTCAACC CTACAGATGT TGTTGTGGCA GCTGGGGAAC CTGCAATTTT GGAGTGTCAA
    CCCCCCAGAG GACATCCGGA ACCAACTATA TACTGGAAAA AGGACAAAGT TAGAATTGAT
    GATAAAGATG AAAGAATTAG TATTCGAGGT GGAAAGCTGA TGCTCTCCAA TACAAGGAAA
    AGTGATGCAG GCATGTACAC TTGTGTTGGG ACCAACATGG TTGGAGAAAG AGACAGTGAC
    CCAGCAGAGC TAACAGTCTT TGAACGTCCC ACATTCCTCA GAAGGCCAAT TAATCAAGTT
    GTTTTAGAGG ATGAAGCTGT AGAATTTCGA TGTCAGGTCC AGGGGGACCC ACAGCCAACT
    GTCCGGTGGA AAAAGGATGA TGTAGATTTG CCAAGAGGAA GATACGACAT CAAGGATGAC
    TATACTTTGA GAATCAAGAA GGCTTCCAGC AGCGACGAAG GGACCTTTGC TTGTATTTCT
    GAAAACCGGG TTGGTAAAAT AGAAGCTTCT GCCACACTTA CAGTCAGAGT TCGCCCTGTT
    GCTCCTCCCC AGTTTGTGAT CAGACCAAGG GATCAGATTG TAGCACAAGG CCGGACAGTA
    ACATTTGGAT GTGAAGCTAA AGGAAATCCT CAACCAGCTG TTTTTTGGCA AAAAGAAGGA
    AGCCAGAATT TACTTTTTCC AAATCAGCCT CTTCAGCCCA ACAGCAGGTA CTCAGTGTCA
    CCAACTGGAG ATTTAACCAT CACCAGCATT CAGCGCTCAG ACGCAGGCTA CTACATTTGT
    CAGGCACTGA CTGTTGCCGG AAGCATTCTG GCCAAGGCCC AGCTGGAAGT GACTGATGTT
    TTGACAGATA GACCACCGCC AATAATTCTA CAAGGTCCAG CTAATCAAAC TCTTGCCGTG
    GATGGAACAG CAGTGCTGAA ATGTAAAACT ACTGGGGACC CAATGCCAGT CATAAGTTGG
    CTAAAAGAGG GATTTAAGGG ATTCATAGGG AGAGATGCAA GGTTATCCAT TCTAGACCAA
    GGGACATTAC AAATTAAAGG TTTAAAGGTT ACTGATTCTG GCACCTATAC TTGTGTGGCT
    ACTAGCTCTA GTGGTGAGAC AACATGGAGC GCTGTTCTGG AAGTGACAGA ATCTGGTGGT
    GCCATAATTA GCAAGAACTA TGATCTCAAT GACCTTCCTG GATCTCCATC AAAACCTCAG
    GTTACAGATG TCAGTAAGAA CAGTGTTACC CTATCATGGC AACCAGGATC ACCAGGGATT
    CTGCCTGTGA CATCCTACAT CATTGAGGCT TTCAGTCAAT CTGTGAGCAA TAGTTGGCAA
    ACTGTGGCCA ATCACGTGAA GACAACGCTT TACACTGTTA AAGGACTGCG CCCAAACACC
    ATTTATCTCT TTATGGTGAG AGCAATCAAT ACTGAAGGAC TGAGTGACCC AAGTCCAATA
    TCTGATCCAG TGCGTACACA AGATATAAGT CCACCTGTTC AAGGAGTAGA TCACCGGCAA
    GTACAGAAAG AACTTGGGGA TGTGATTGTA CGGCTCCATA ATCCTGTTGT TCTGACACCA
    ACAACAATCC AGGTCACCTG GATGGTCGAC CGTCAATCCC AGTTTATTCA GGGATACAGG
    GTAATGTACC GTCAGACTTC TGGTCTGCAG ACTCCATCTG CGTGGCAGAG CTTAGAGGTC
    AAAGTGCCTG CAGAGCGTAG CGCAGTGCTA ACCAACCTGA AAAAAGGAAT TACTTATGAG
    ATTAAGGTTC GACCTTATTT TAATGAATTT CAAGGAATGG ATAGCGAGTC AAAGTCAGCA
    CGAACAACTG AAGAAGCCCC AAGTGCTCCT CCTCAGTCTG TTACTGTTAT GACAGTGGGA
    AATAACAACA GCACCAGCAT CAGCATCTCA TGGGACCCCC CTCCATCTGA CCACCAGAAT
    GGAATTATCC AGGACTACAG GATCTGGTGC CTGGGTAATG AGACACGTTT CCACATCAAC
    AAGACAGTGG ATGCTGCTAT TCGTTCCGTG GTTATCGGTG GGCTATTCCC TGGAATTCAG
    TACCGCGTTG AAGTCGCAGC CAGTACAAGC GCAGGCACTG GGGTAAAAAG TGCTCCCCAG
    CACATACAGA TAGGAAATCG GAATGAAGTT GTGATCACAG AGAATAACAA CAGCATAACA
    GAGCAAATAA CTGATGTTGT GAAACAGCCG GCATTCATCG CAGGAATTGG TGGTGCCTGC
    TGGGTAATAC TCATGGGATT TAGCATTTGG CTCTACTGGC GAAGAAAGAA GAGAAAGGGT
    CTCAGCAACT ACGCTGTTCA GTCTTTCACC TTTACCCCTG CAGTTACATT CCAGAGAGGA
    GATGGAGGAC TAATGAGTAA TGGAAGTCGT CCTGGACTTT TAAATGCGAA TGATCCTAGT
    TACCCTTGGT TGGCAGACTC CTGGCCAGCT TCCAGCATGC CGGTGAATAA CAGCAACAAT
    GGACCAAACG ATCTCGTTAA TTTTAGTTGT GGAGATGTGC TGCCAACAGT TCCAGGTCAA
    GGAGAAAAAA CAGCGACTAT GCTTTCTGAT GGAGCCATTT ATAGCAGCAT TGATTTTACT
    ACAAAATCTA GCTATAATTG CCCAGGACAA ATTGCACAAA CCACGCCATA TGCTACTACG
    CAGATACTAC ATTCCAACAG CATTCATGAG CTGGCAGTTG ACTTGCCTGA TCCACAGTGG
    AAATCTTCCA TTCAGCAGAA ATCTGATCTC ACTGGATTAG GCTATTGCCT TACTGACCAA
    GGCAAAGGGA GTAATGCCTT ACTTTACATT CCTGACTATC GAATGGCTGA GGGATTGTCA
    AATAGAATAC CCCACAACCA GTCACAGGAT TTCAGCACCA CCAGCTCTCA CAACAGCTCA
    GAGAGAAGTG GCAGCCTTTC AGGTGGAAAA GACGGAAAAA GGAAGAAAAA TAAAAACAGC
    TCTAAAATCT CTAAAAACAA TGGATCCAGT TGGGCCAATG TTCCACTTCC GCCCCCTCCC
    ATCCAACCAC TGCCAGGCAC TGAAATAGAA CATTATGGCC TTGAACACCA GGAAACAGGC
    TATGACACCA ATGGATGGTG CCCACCTTTG CCAGTCCAGA CCTACTTACA TCAAGGATCA
    GATGATGAAC TAGAAGAAGA TGAAGAGAGA GTCCCTACAC CCCCTGTACG TGGGGTGGCA
    TCATCACCTG CCATCTCATT TGGACAACAG TCAACAGCAA CCCTCACACC ATCACCACGT
    GAAGAAATGC AACCCATGCT TCAGGCCCAC CTGGATGAAT TAACAAGAGC TTACCAGTTT
    GACATTGCAA AGCAATCTTG GCATTTACAC AGCAATAACC AGCCCCCATT GGCCCCAGTT
    CCACCACTTG GATATGTGTC TGGGACATTT ATTTCTGACT TAGAAAAAGA CATTCCAGAT
    CAAGAAGAAG ATGAAATTGA AGACGAATCT ATAGAAATAA CAAGGCCCCT CCGAGGTTTG
    GAACACACTC CCGGTTCTAG TATGGATAAT CTCGACAGTT CTGTGACAGG TTCAATGGTA
    AATGGATGGG GTTCTGCATC TGATGAGGAT CGGAACTATT CTAGTCATAG ATCTAGTGTA
    GGTAGCGCAA CAGATGACTC GATAATTGCA AGTGGCAACT TTGCACAATC ACTGGTTGCA
    GCAGCAGATA AAGCTGGTTT CTCACTAAAT GGAAACAGCC TATCTATAGC AGGCAAAATG
    AACTCCTTTT CTCAGAGACC CCGGCCTGCC AGTCCCTTTT CTACTGACAG TAACACAAGT
    TCAGCTGTGA ATCATAGTCA GAAGTCACGG CCCACTAAGA AACACAAGGG AGGCAGAATA
    GGTCAGCAAC CCATGCTTCG CAGAGAAGGG ATTGCAGATG ATTTGCCTCC CCCACCTGAT
    CCTCCACCTT GTCAAAGACA GCAAATCATG CCCAGTCAAC GTGGTGGAGG TCCTACAGAA
    AGAAAAGCCA GTTCTCTTGA GAGGCACACA TCAAATATGA ATGATTTAAA AACCTCATTA
    GACCGGCATG GCAGAACATC CTTAGAACGG CAGCGACAAA CACAGGAATG GCTTGGCTCA
    ACAGAAAGGC AGAACAAGCA GGCATTTGGA TCTGAAGAGA TACTAATGCC ATACAGTAAA
    CCTAGCTTCC CATCTCCAGG AGGGCACAGC TCCTCAGGAA CAGTCTCTTC AAAAGAATCA
    ACTGGACCCA GAAAAGGGGA GGTTTTGAGA GGTCACCAAC GTAATGCCAT TGACTTTCTT
    GATGTAGGAT ATTTGGGGCC ACACAGTCAA GGACAGTTTA CAGGTGAATT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752456.1
    CDS546..5339
    Translation

    Target ORF information:

    RefSeq Version XM_031896596.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896596.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAACATCT GGATAATGCT ATCTTCACTA AGGCAGAGGG CAGCTTCTCC TGAATTCCTG 
    CTGATCCTTA TGCTGTTCGT CAGTGGGGTG ACCCAAAGCA AAATTAGTAG GACCAAGGGG
    AAGCACTGGA AAGGGGGATC CCGTCTCCGT CAAGAAGATT TCCCCCCACG GATAGTTGAG
    CATCCATCGG ATGTCATTGT GTCAAAGGGA GAGCCAACCA CCTTGAACTG CAAAGCGGAA
    GGGCGGCCTA CACCCACCAT AGAGTGGTAC AAAGACTTAG AAAGGGTTGA GACAGACAAG
    GATGACCCCC GCTCACATCG AATGCTACTG CCAAGTGGAT CTTTATTTTT TCTGCGCATT
    ATCCATGGGC GCAAGTTTAA GCCAGACGAA GGAAGTTACG TCTGTGTGGC AAGGAACTAT
    CTCGGGGAGG CTATGAGTCG AAATGCATCT CTGGAAGTGG CATTGCTCCG TGATGACTTC
    CGGCTCAACC CTACAGATGT TGTTGTGGCA GCTGGGGAAC CTGCAATTTT GGAGTGTCAA
    CCCCCCAGAG GACATCCGGA ACCAACTATA TACTGGAAAA AGGACAAAGT TAGAATTGAT
    GATAAAGATG AAAGAATTAG TATTCGAGGT GGAAAGCTGA TGCTCTCCAA TACAAGGAAA
    AGTGATGCAG GCATGTACAC TTGTGTTGGG ACCAACATGG TTGGAGAAAG AGACAGTGAC
    CCAGCAGAGC TAACAGTCTT TGAACGTCCC ACATTCCTCA GAAGGCCAAT TAATCAAGTT
    GTTTTAGAGG ATGAAGCTGT AGAATTTCGA TGTCAGGTCC AGGGGGACCC ACAGCCAACT
    GTCCGGTGGA AAAAGGATGA TGTAGATTTG CCAAGAGGAA GATACGACAT CAAGGATGAC
    TATACTTTGA GAATCAAGAA GGCTTCCAGC AGCGACGAAG GGACCTTTGC TTGTATTTCT
    GAAAACCGGG TTGGTAAAAT AGAAGCTTCT GCCACACTTA CAGTCAGAGT TCGCCCTGTT
    GCTCCTCCCC AGTTTGTGAT CAGACCAAGG GATCAGATTG TAGCACAAGG CCGGACAGTA
    ACATTTGGAT GTGAAGCTAA AGGAAATCCT CAACCAGCTG TTTTTTGGCA AAAAGAAGGA
    AGCCAGAATT TACTTTTTCC AAATCAGCCT CTTCAGCCCA ACAGCAGGTA CTCAGTGTCA
    CCAACTGGAG ATTTAACCAT CACCAGCATT CAGCGCTCAG ACGCAGGCTA CTACATTTGT
    CAGGCACTGA CTGTTGCCGG AAGCATTCTG GCCAAGGCCC AGCTGGAAGT GACTGATGTT
    TTGACAGATA GACCACCGCC AATAATTCTA CAAGGTCCAG CTAATCAAAC TCTTGCCGTG
    GATGGAACAG CAGTGCTGAA ATGTAAAACT ACTGGGGACC CAATGCCAGT CATAAGTTGG
    CTAAAAGAGG GATTTAAGGG ATTCATAGGG AGAGATGCAA GGTTATCCAT TCTAGACCAA
    GGGACATTAC AAATTAAAGG TTTAAAGGTT ACTGATTCTG GCACCTATAC TTGTGTGGCT
    ACTAGCTCTA GTGGTGAGAC AACATGGAGC GCTGTTCTGG AAGTGACAGA ATCTGGTGGT
    GCCATAATTA GCAAGAACTA TGATCTCAAT GACCTTCCTG GATCTCCATC AAAACCTCAG
    GTTACAGATG TCAGTAAGAA CAGTGTTACC CTATCATGGC AACCAGGATC ACCAGGGATT
    CTGCCTGTGA CATCCTACAT CATTGAGGCT TTCAGTCAAT CTGTGAGCAA TAGTTGGCAA
    ACTGTGGCCA ATCACGTGAA GACAACGCTT TACACTGTTA AAGGACTGCG CCCAAACACC
    ATTTATCTCT TTATGGTGAG AGCAATCAAT ACTGAAGGAC TGAGTGACCC AAGTCCAATA
    TCTGATCCAG TGCGTACACA AGATATAAGT CCACCTGTTC AAGGAGTAGA TCACCGGCAA
    GTACAGAAAG AACTTGGGGA TGTGATTGTA CGGCTCCATA ATCCTGTTGT TCTGACACCA
    ACAACAATCC AGGTCACCTG GATGGTCGAC CGTCAATCCC AGTTTATTCA GGGATACAGG
    GTAATGTACC GTCAGACTTC TGGTCTGCAG ACTCCATCTG CGTGGCAGAG CTTAGAGGTC
    AAAGTGCCTG CAGAGCGTAG CGCAGTGCTA ACCAACCTGA AAAAAGGAAT TACTTATGAG
    ATTAAGGTTC GACCTTATTT TAATGAATTT CAAGGAATGG ATAGCGAGTC AAAGTCAGCA
    CGAACAACTG AAGAAGCCCC AAGTGCTCCT CCTCAGTCTG TTACTGTTAT GACAGTGGGA
    AATAACAACA GCACCAGCAT CAGCATCTCA TGGGACCCCC CTCCATCTGA CCACCAGAAT
    GGAATTATCC AGGACTACAG GATCTGGTGC CTGGGTAATG AGACACGTTT CCACATCAAC
    AAGACAGTGG ATGCTGCTAT TCGTTCCGTG GTTATCGGTG GGCTATTCCC TGGAATTCAG
    TACCGCGTTG AAGTCGCAGC CAGTACAAGC GCAGGCACTG GGGTAAAAAG TGCTCCCCAG
    CACATACAGA TAGGAAATCG GAATGAAGTT GTGATCACAG AGAATAACAA CAGCATAACA
    GAGCAAATAA CTGATGTTGT GAAACAGCCG GCATTCATCG CAGGAATTGG TGGTGCCTGC
    TGGGTAATAC TCATGGGATT TAGCATTTGG CTCTACTGGC GAAGAAAGAA GAGAAAGGGT
    CTCAGCAACT ACGCTGTTCA GTCTTTCACC TTTACCCCTG CAGTTACATT CCAGAGAGGA
    GATGGAGGAC TAATGAGTAA TGGAAGTCGT CCTGGACTTT TAAATGCGAA TGATCCTAGT
    TACCCTTGGT TGGCAGACTC CTGGCCAGCT TCCAGCATGC CGGTGAATAA CAGCAACAAT
    GGACCAAACG ATCTCGTTAA TTTTAGTTGT GGAGATGTGC TGCCAACAGT TCCAGGTCAA
    GGAGAAAAAA CAGCGACTAT GCTTTCTGAT GGAGCCATTT ATAGCAGCAT TGATTTTACT
    ACAAAATCTA GCTATAATTG CCCAGGACAA ATTGCACAAA CCACGCCATA TGCTACTACG
    CAGATACTAC ATTCCAACAG CATTCATGAG CTGGCAGTTG ACTTGCCTGA TCCACAGTGG
    AAATCTTCCA TTCAGCAGAA ATCTGATCTC ACTGGATTAG GCTATTGCCT TACTGACCAA
    GGCAAAGGGA GTAATGCCTT ACTTTACATT CCTGACTATC GAATGGCTGA GGGATTGTCA
    AATAGAATAC CCCACAACCA GTCACAGGAT TTCAGCACCA CCAGCTCTCA CAACAGCTCA
    GAGAGAAGTG GCAGCCTTTC AGGTGGAAAA GACGGAAAAA GGAAGAAAAA TAAAAACAGC
    TCTAAAATCT CTAAAAACAA TGGATCCAGT TGGGCCAATG TTCCACTTCC GCCCCCTCCC
    ATCCAACCAC TGCCAGGCAC TGAAATAGAA CATTATGGCC TTGAACACCA GGAAACAGGC
    TATGACACCA ATGGATGGTG CCCACCTTTG CCAGTCCAGA CCTACTTACA TCAAGGATCA
    GATGATGAAC TAGAAGAAGA TGAAGAGAGA GTCCCTACAC CCCCTGTACG TGGGGTGGCA
    TCATCACCTG CCATCTCATT TGGACAACAG TCAACAGCAA CCCTCACACC ATCACCACGT
    GAAGAAATGC AACCCATGCT TCAGGCCCAC CTGGATGAAT TAACAAGAGC TTACCAGTTT
    GACATTGCAA AGCAATCTTG GCATTTACAC AGCAATAACC AGCCCCCATT GGCCCCAGTT
    CCACCACTTG GATATGTGTC TGGGACATTT ATTTCTGACT TAGAAAAAGA CATTCCAGAT
    CAAGAAGAAG ATGAAATTGA AGACGAATCT ATAGAAATAA CAAGGCCCCT CCGAGGTTTG
    GAACACACTC CCGGTTCTAG TATGGATAAT CTCGACAGTT CTGTGACAGG TTCAATGGTA
    AATGGATGGG GTTCTGCATC TGATGAGGAT CGGAACTATT CTAGTCATAG ATCTAGTGTA
    GGTAGCGCAA CAGATGACTC GATAATTGCA AGTGGCAACT TTGCACAATC ACTGGTTGCA
    GCAGCAGATA AAGCTGGTTT CTCACTAAAT GGAAACAGCC TATCTATAGC AGGCAAAATG
    AACTCCTTTT CTCAGAGACC CCGGCCTGCC AGTCCCTTTT CTACTGACAG TAACACAAGT
    TCAGCTGTGA ATCATAGTCA GAAGTCACGG CCCACTAAGA AACACAAGGG AGGCAGAATA
    GGTCAGCAAC CCATGCTTCG CAGAGAAGGG ATTGCAGATG ATTTGCCTCC CCCACCTGAT
    CCTCCACCTT GTCAAAGACA GCAAATCATG CCCAGTCAAC GTGGTGGAGG TCCTACAGAA
    AGAAAAGCCA GTTCTCTTGA GAGGCACACA TCAAATATGA ATGATTTAAA AACCTCATTA
    GACCGGCATG GCAGAACATC CTTAGAACGG CAGCGACAAA CACAGGAATG GCTTGGCTCA
    ACAGAAAGGC AGAACAAGCA GGCATTTGGA TCTGAAGAGA TACTAATGCC ATACAGTAAA
    CCTAGCTTCC CATCTCCAGG AGGGCACAGC TCCTCAGGAA CAGTCTCTTC AAAAGAATCA
    ACTGGACCCA GAAAAGGGGA GGTTTTGAGA GGTCACCAAC GTAATGCCAT TGACTTTCTT
    GATGTAGGAT ATTTGGGGCC ACACAGTCAA GGACAGTTTA CAGGTGAATT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa51974
    Clone ID Related Accession (Same CDS sequence) XM_031896597.1
    Accession Version XM_031896597.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4782bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product roundabout homolog 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGCTTCC TGGTGGTGCC CCTGCTGTGT GGATTGTTAT GCTGTCAGGT GTGGGGATCC 
    CGTCTCCGTC AAGAAGATTT CCCCCCACGG ATAGTTGAGC ATCCATCGGA TGTCATTGTG
    TCAAAGGGAG AGCCAACCAC CTTGAACTGC AAAGCGGAAG GGCGGCCTAC ACCCACCATA
    GAGTGGTACA AAGACTTAGA AAGGGTTGAG ACAGACAAGG ATGACCCCCG CTCACATCGA
    ATGCTACTGC CAAGTGGATC TTTATTTTTT CTGCGCATTA TCCATGGGCG CAAGTTTAAG
    CCAGACGAAG GAAGTTACGT CTGTGTGGCA AGGAACTATC TCGGGGAGGC TATGAGTCGA
    AATGCATCTC TGGAAGTGGC ATTGCTCCGT GATGACTTCC GGCTCAACCC TACAGATGTT
    GTTGTGGCAG CTGGGGAACC TGCAATTTTG GAGTGTCAAC CCCCCAGAGG ACATCCGGAA
    CCAACTATAT ACTGGAAAAA GGACAAAGTT AGAATTGATG ATAAAGATGA AAGAATTAGT
    ATTCGAGGTG GAAAGCTGAT GCTCTCCAAT ACAAGGAAAA GTGATGCAGG CATGTACACT
    TGTGTTGGGA CCAACATGGT TGGAGAAAGA GACAGTGACC CAGCAGAGCT AACAGTCTTT
    GAACGTCCCA CATTCCTCAG AAGGCCAATT AATCAAGTTG TTTTAGAGGA TGAAGCTGTA
    GAATTTCGAT GTCAGGTCCA GGGGGACCCA CAGCCAACTG TCCGGTGGAA AAAGGATGAT
    GTAGATTTGC CAAGAGGAAG ATACGACATC AAGGATGACT ATACTTTGAG AATCAAGAAG
    GCTTCCAGCA GCGACGAAGG GACCTTTGCT TGTATTTCTG AAAACCGGGT TGGTAAAATA
    GAAGCTTCTG CCACACTTAC AGTCAGAGTT CGCCCTGTTG CTCCTCCCCA GTTTGTGATC
    AGACCAAGGG ATCAGATTGT AGCACAAGGC CGGACAGTAA CATTTGGATG TGAAGCTAAA
    GGAAATCCTC AACCAGCTGT TTTTTGGCAA AAAGAAGGAA GCCAGAATTT ACTTTTTCCA
    AATCAGCCTC TTCAGCCCAA CAGCAGGTAC TCAGTGTCAC CAACTGGAGA TTTAACCATC
    ACCAGCATTC AGCGCTCAGA CGCAGGCTAC TACATTTGTC AGGCACTGAC TGTTGCCGGA
    AGCATTCTGG CCAAGGCCCA GCTGGAAGTG ACTGATGTTT TGACAGATAG ACCACCGCCA
    ATAATTCTAC AAGGTCCAGC TAATCAAACT CTTGCCGTGG ATGGAACAGC AGTGCTGAAA
    TGTAAAACTA CTGGGGACCC AATGCCAGTC ATAAGTTGGC TAAAAGAGGG ATTTAAGGGA
    TTCATAGGGA GAGATGCAAG GTTATCCATT CTAGACCAAG GGACATTACA AATTAAAGGT
    TTAAAGGTTA CTGATTCTGG CACCTATACT TGTGTGGCTA CTAGCTCTAG TGGTGAGACA
    ACATGGAGCG CTGTTCTGGA AGTGACAGAA TCTGGTGGTG CCATAATTAG CAAGAACTAT
    GATCTCAATG ACCTTCCTGG ATCTCCATCA AAACCTCAGG TTACAGATGT CAGTAAGAAC
    AGTGTTACCC TATCATGGCA ACCAGGATCA CCAGGGATTC TGCCTGTGAC ATCCTACATC
    ATTGAGGCTT TCAGTCAATC TGTGAGCAAT AGTTGGCAAA CTGTGGCCAA TCACGTGAAG
    ACAACGCTTT ACACTGTTAA AGGACTGCGC CCAAACACCA TTTATCTCTT TATGGTGAGA
    GCAATCAATA CTGAAGGACT GAGTGACCCA AGTCCAATAT CTGATCCAGT GCGTACACAA
    GATATAAGTC CACCTGTTCA AGGAGTAGAT CACCGGCAAG TACAGAAAGA ACTTGGGGAT
    GTGATTGTAC GGCTCCATAA TCCTGTTGTT CTGACACCAA CAACAATCCA GGTCACCTGG
    ATGGTCGACC GTCAATCCCA GTTTATTCAG GGATACAGGG TAATGTACCG TCAGACTTCT
    GGTCTGCAGA CTCCATCTGC GTGGCAGAGC TTAGAGGTCA AAGTGCCTGC AGAGCGTAGC
    GCAGTGCTAA CCAACCTGAA AAAAGGAATT ACTTATGAGA TTAAGGTTCG ACCTTATTTT
    AATGAATTTC AAGGAATGGA TAGCGAGTCA AAGTCAGCAC GAACAACTGA AGAAGCCCCA
    AGTGCTCCTC CTCAGTCTGT TACTGTTATG ACAGTGGGAA ATAACAACAG CACCAGCATC
    AGCATCTCAT GGGACCCCCC TCCATCTGAC CACCAGAATG GAATTATCCA GGACTACAGG
    ATCTGGTGCC TGGGTAATGA GACACGTTTC CACATCAACA AGACAGTGGA TGCTGCTATT
    CGTTCCGTGG TTATCGGTGG GCTATTCCCT GGAATTCAGT ACCGCGTTGA AGTCGCAGCC
    AGTACAAGCG CAGGCACTGG GGTAAAAAGT GCTCCCCAGC ACATACAGAT AGGAAATCGG
    AATGAAGTTG TGATCACAGA GAATAACAAC AGCATAACAG AGCAAATAAC TGATGTTGTG
    AAACAGCCGG CATTCATCGC AGGAATTGGT GGTGCCTGCT GGGTAATACT CATGGGATTT
    AGCATTTGGC TCTACTGGCG AAGAAAGAAG AGAAAGGGTC TCAGCAACTA CGCTGTTCAG
    TCTTTCACCT TTACCCCTGC AGATTCCCCA GATACAACCA TGCACCACAA GGTTGCACTT
    GGCACAAAGA AGGGGAGCTG GCACAAAACA GTTACATTCC AGAGAGGAGA TGGAGGACTA
    ATGAGTAATG GAAGTCGTCC TGGACTTTTA AATGCGAATG ATCCTAGTTA CCCTTGGTTG
    GCAGACTCCT GGCCAGCTTC CAGCATGCCG GTGAATAACA GCAACAATGG ACCAAACGAT
    CTCGTTAATT TTAGTTGTGG AGATGTGCTG CCAACAGTTC CAGGTCAAGG AGAAAAAACA
    GCGACTATGC TTTCTGATGG AGCCATTTAT AGCAGCATTG ATTTTACTAC AAAATCTAGC
    TATAATTGCC CAGGACAAAT TGCACAAACC ACGCCATATG CTACTACGCA GATACTACAT
    TCCAACAGCA TTCATGAGCT GGCAGTTGAC TTGCCTGATC CACAGTGGAA ATCTTCCATT
    CAGCAGAAAT CTGATCTCAC TGGATTAGGC TATTGCCTTA CTGACCAAGG CAAAGGGAGT
    AATGCCTTAC TTTACATTCC TGACTATCGA ATGGCTGAGG GATTGTCAAA TAGAATACCC
    CACAACCAGT CACAGGATTT CAGCACCACC AGCTCTCACA ACAGCTCAGA GAGAAGTGGC
    AGCCTTTCAG GTGGAAAAGA CGGAAAAAGG AAGAAAAATA AAAACAGCTC TAAAATCTCT
    AAAAACAATG GATCCAGTTG GGCCAATGTT CCACTTCCGC CCCCTCCCAT CCAACCACTG
    CCAGGCACTG AAATAGAACA TTATGGCCTT GAACACCAGG AAACAGGCTA TGACACCAAT
    GGATGGTGCC CACCTTTGCC AGTCCAGACC TACTTACATC AAGGATCAGA TGATGAACTA
    GAAGAAGATG AAGAGAGAGT CCCTACACCC CCTGTACGTG GGGTGGCATC ATCACCTGCC
    ATCTCATTTG GACAACAGTC AACAGCAACC CTCACACCAT CACCACGTGA AGAAATGCAA
    CCCATGCTTC AGGCCCACCT GGATGAATTA ACAAGAGCTT ACCAGTTTGA CATTGCAAAG
    CAATCTTGGC ATTTACACAG CAATAACCAG CCCCCATTGG CCCCAGTTCC ACCACTTGGA
    TATGTGTCTG GGACATTTAT TTCTGACTTA GAAAAAGACA TTCCAGATCA AGAAGAAGAT
    GAAATTGAAG ACGAATCTAT AGAAATAACA AGGCCCCTCC GAGGTTTGGA ACACACTCCC
    GGTTCTAGTA TGGATAATCT CGACAGTTCT GTGACAGGTT CAATGGTAAA TGGATGGGGT
    TCTGCATCTG ATGAGGATCG GAACTATTCT AGTCATAGAT CTAGTGTAGG TAGCGCAACA
    GATGACTCGA TAATTGCAAG TGGCAACTTT GCACAATCAC TGGTTGCAGC AGCAGATAAA
    GCTGGTTTCT CACTAAATGG AAACAGCCTA TCTATAGCAG GCAAAATGAA CTCCTTTTCT
    CAGAGACCCC GGCCTGCCAG TCCCTTTTCT ACTGACAGTA ACACAAGTTC AGCTGTGAAT
    CATAGTCAGA AGTCACGGCC CACTAAGAAA CACAAGGGAG GCAGAATAGG TCAGCAACCC
    ATGCTTCGCA GAGAAGGGAT TGCAGATGAT TTGCCTCCCC CACCTGATCC TCCACCTTGT
    CAAAGACAGC AAATCATGCC CAGTCAACGT GGTGGAGGTC CTACAGAAAG AAAAGCCAGT
    TCTCTTGAGA GGCACACATC AAATATGAAT GATTTAAAAA CCTCATTAGA CCGGCATGGC
    AGAACATCCT TAGAACGGCA GCGACAAACA CAGGAATGGC TTGGCTCAAC AGAAAGGCAG
    AACAAGCAGG CATTTGGATC TGAAGAGATA CTAATGCCAT ACAGTAAACC TAGCTTCCCA
    TCTCCAGGAG GGCACAGCTC CTCAGGAACA GTCTCTTCAA AAGAATCAAC TGGACCCAGA
    AAAGGGGAGG TTTTGAGAGG TCACCAACGT AATGCCATTG ACTTTCTTGA TGTAGGATAT
    TTGGGGCCAC ACAGTCAAGG ACAGTTTACA GGTGAATTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752457.1
    CDS763..5544
    Translation

    Target ORF information:

    RefSeq Version XM_031896597.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896597.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGCTTCC TGGTGGTGCC CCTGCTGTGT GGATTGTTAT GCTGTCAGGT GTGGGGATCC 
    CGTCTCCGTC AAGAAGATTT CCCCCCACGG ATAGTTGAGC ATCCATCGGA TGTCATTGTG
    TCAAAGGGAG AGCCAACCAC CTTGAACTGC AAAGCGGAAG GGCGGCCTAC ACCCACCATA
    GAGTGGTACA AAGACTTAGA AAGGGTTGAG ACAGACAAGG ATGACCCCCG CTCACATCGA
    ATGCTACTGC CAAGTGGATC TTTATTTTTT CTGCGCATTA TCCATGGGCG CAAGTTTAAG
    CCAGACGAAG GAAGTTACGT CTGTGTGGCA AGGAACTATC TCGGGGAGGC TATGAGTCGA
    AATGCATCTC TGGAAGTGGC ATTGCTCCGT GATGACTTCC GGCTCAACCC TACAGATGTT
    GTTGTGGCAG CTGGGGAACC TGCAATTTTG GAGTGTCAAC CCCCCAGAGG ACATCCGGAA
    CCAACTATAT ACTGGAAAAA GGACAAAGTT AGAATTGATG ATAAAGATGA AAGAATTAGT
    ATTCGAGGTG GAAAGCTGAT GCTCTCCAAT ACAAGGAAAA GTGATGCAGG CATGTACACT
    TGTGTTGGGA CCAACATGGT TGGAGAAAGA GACAGTGACC CAGCAGAGCT AACAGTCTTT
    GAACGTCCCA CATTCCTCAG AAGGCCAATT AATCAAGTTG TTTTAGAGGA TGAAGCTGTA
    GAATTTCGAT GTCAGGTCCA GGGGGACCCA CAGCCAACTG TCCGGTGGAA AAAGGATGAT
    GTAGATTTGC CAAGAGGAAG ATACGACATC AAGGATGACT ATACTTTGAG AATCAAGAAG
    GCTTCCAGCA GCGACGAAGG GACCTTTGCT TGTATTTCTG AAAACCGGGT TGGTAAAATA
    GAAGCTTCTG CCACACTTAC AGTCAGAGTT CGCCCTGTTG CTCCTCCCCA GTTTGTGATC
    AGACCAAGGG ATCAGATTGT AGCACAAGGC CGGACAGTAA CATTTGGATG TGAAGCTAAA
    GGAAATCCTC AACCAGCTGT TTTTTGGCAA AAAGAAGGAA GCCAGAATTT ACTTTTTCCA
    AATCAGCCTC TTCAGCCCAA CAGCAGGTAC TCAGTGTCAC CAACTGGAGA TTTAACCATC
    ACCAGCATTC AGCGCTCAGA CGCAGGCTAC TACATTTGTC AGGCACTGAC TGTTGCCGGA
    AGCATTCTGG CCAAGGCCCA GCTGGAAGTG ACTGATGTTT TGACAGATAG ACCACCGCCA
    ATAATTCTAC AAGGTCCAGC TAATCAAACT CTTGCCGTGG ATGGAACAGC AGTGCTGAAA
    TGTAAAACTA CTGGGGACCC AATGCCAGTC ATAAGTTGGC TAAAAGAGGG ATTTAAGGGA
    TTCATAGGGA GAGATGCAAG GTTATCCATT CTAGACCAAG GGACATTACA AATTAAAGGT
    TTAAAGGTTA CTGATTCTGG CACCTATACT TGTGTGGCTA CTAGCTCTAG TGGTGAGACA
    ACATGGAGCG CTGTTCTGGA AGTGACAGAA TCTGGTGGTG CCATAATTAG CAAGAACTAT
    GATCTCAATG ACCTTCCTGG ATCTCCATCA AAACCTCAGG TTACAGATGT CAGTAAGAAC
    AGTGTTACCC TATCATGGCA ACCAGGATCA CCAGGGATTC TGCCTGTGAC ATCCTACATC
    ATTGAGGCTT TCAGTCAATC TGTGAGCAAT AGTTGGCAAA CTGTGGCCAA TCACGTGAAG
    ACAACGCTTT ACACTGTTAA AGGACTGCGC CCAAACACCA TTTATCTCTT TATGGTGAGA
    GCAATCAATA CTGAAGGACT GAGTGACCCA AGTCCAATAT CTGATCCAGT GCGTACACAA
    GATATAAGTC CACCTGTTCA AGGAGTAGAT CACCGGCAAG TACAGAAAGA ACTTGGGGAT
    GTGATTGTAC GGCTCCATAA TCCTGTTGTT CTGACACCAA CAACAATCCA GGTCACCTGG
    ATGGTCGACC GTCAATCCCA GTTTATTCAG GGATACAGGG TAATGTACCG TCAGACTTCT
    GGTCTGCAGA CTCCATCTGC GTGGCAGAGC TTAGAGGTCA AAGTGCCTGC AGAGCGTAGC
    GCAGTGCTAA CCAACCTGAA AAAAGGAATT ACTTATGAGA TTAAGGTTCG ACCTTATTTT
    AATGAATTTC AAGGAATGGA TAGCGAGTCA AAGTCAGCAC GAACAACTGA AGAAGCCCCA
    AGTGCTCCTC CTCAGTCTGT TACTGTTATG ACAGTGGGAA ATAACAACAG CACCAGCATC
    AGCATCTCAT GGGACCCCCC TCCATCTGAC CACCAGAATG GAATTATCCA GGACTACAGG
    ATCTGGTGCC TGGGTAATGA GACACGTTTC CACATCAACA AGACAGTGGA TGCTGCTATT
    CGTTCCGTGG TTATCGGTGG GCTATTCCCT GGAATTCAGT ACCGCGTTGA AGTCGCAGCC
    AGTACAAGCG CAGGCACTGG GGTAAAAAGT GCTCCCCAGC ACATACAGAT AGGAAATCGG
    AATGAAGTTG TGATCACAGA GAATAACAAC AGCATAACAG AGCAAATAAC TGATGTTGTG
    AAACAGCCGG CATTCATCGC AGGAATTGGT GGTGCCTGCT GGGTAATACT CATGGGATTT
    AGCATTTGGC TCTACTGGCG AAGAAAGAAG AGAAAGGGTC TCAGCAACTA CGCTGTTCAG
    TCTTTCACCT TTACCCCTGC AGATTCCCCA GATACAACCA TGCACCACAA GGTTGCACTT
    GGCACAAAGA AGGGGAGCTG GCACAAAACA GTTACATTCC AGAGAGGAGA TGGAGGACTA
    ATGAGTAATG GAAGTCGTCC TGGACTTTTA AATGCGAATG ATCCTAGTTA CCCTTGGTTG
    GCAGACTCCT GGCCAGCTTC CAGCATGCCG GTGAATAACA GCAACAATGG ACCAAACGAT
    CTCGTTAATT TTAGTTGTGG AGATGTGCTG CCAACAGTTC CAGGTCAAGG AGAAAAAACA
    GCGACTATGC TTTCTGATGG AGCCATTTAT AGCAGCATTG ATTTTACTAC AAAATCTAGC
    TATAATTGCC CAGGACAAAT TGCACAAACC ACGCCATATG CTACTACGCA GATACTACAT
    TCCAACAGCA TTCATGAGCT GGCAGTTGAC TTGCCTGATC CACAGTGGAA ATCTTCCATT
    CAGCAGAAAT CTGATCTCAC TGGATTAGGC TATTGCCTTA CTGACCAAGG CAAAGGGAGT
    AATGCCTTAC TTTACATTCC TGACTATCGA ATGGCTGAGG GATTGTCAAA TAGAATACCC
    CACAACCAGT CACAGGATTT CAGCACCACC AGCTCTCACA ACAGCTCAGA GAGAAGTGGC
    AGCCTTTCAG GTGGAAAAGA CGGAAAAAGG AAGAAAAATA AAAACAGCTC TAAAATCTCT
    AAAAACAATG GATCCAGTTG GGCCAATGTT CCACTTCCGC CCCCTCCCAT CCAACCACTG
    CCAGGCACTG AAATAGAACA TTATGGCCTT GAACACCAGG AAACAGGCTA TGACACCAAT
    GGATGGTGCC CACCTTTGCC AGTCCAGACC TACTTACATC AAGGATCAGA TGATGAACTA
    GAAGAAGATG AAGAGAGAGT CCCTACACCC CCTGTACGTG GGGTGGCATC ATCACCTGCC
    ATCTCATTTG GACAACAGTC AACAGCAACC CTCACACCAT CACCACGTGA AGAAATGCAA
    CCCATGCTTC AGGCCCACCT GGATGAATTA ACAAGAGCTT ACCAGTTTGA CATTGCAAAG
    CAATCTTGGC ATTTACACAG CAATAACCAG CCCCCATTGG CCCCAGTTCC ACCACTTGGA
    TATGTGTCTG GGACATTTAT TTCTGACTTA GAAAAAGACA TTCCAGATCA AGAAGAAGAT
    GAAATTGAAG ACGAATCTAT AGAAATAACA AGGCCCCTCC GAGGTTTGGA ACACACTCCC
    GGTTCTAGTA TGGATAATCT CGACAGTTCT GTGACAGGTT CAATGGTAAA TGGATGGGGT
    TCTGCATCTG ATGAGGATCG GAACTATTCT AGTCATAGAT CTAGTGTAGG TAGCGCAACA
    GATGACTCGA TAATTGCAAG TGGCAACTTT GCACAATCAC TGGTTGCAGC AGCAGATAAA
    GCTGGTTTCT CACTAAATGG AAACAGCCTA TCTATAGCAG GCAAAATGAA CTCCTTTTCT
    CAGAGACCCC GGCCTGCCAG TCCCTTTTCT ACTGACAGTA ACACAAGTTC AGCTGTGAAT
    CATAGTCAGA AGTCACGGCC CACTAAGAAA CACAAGGGAG GCAGAATAGG TCAGCAACCC
    ATGCTTCGCA GAGAAGGGAT TGCAGATGAT TTGCCTCCCC CACCTGATCC TCCACCTTGT
    CAAAGACAGC AAATCATGCC CAGTCAACGT GGTGGAGGTC CTACAGAAAG AAAAGCCAGT
    TCTCTTGAGA GGCACACATC AAATATGAAT GATTTAAAAA CCTCATTAGA CCGGCATGGC
    AGAACATCCT TAGAACGGCA GCGACAAACA CAGGAATGGC TTGGCTCAAC AGAAAGGCAG
    AACAAGCAGG CATTTGGATC TGAAGAGATA CTAATGCCAT ACAGTAAACC TAGCTTCCCA
    TCTCCAGGAG GGCACAGCTC CTCAGGAACA GTCTCTTCAA AAGAATCAAC TGGACCCAGA
    AAAGGGGAGG TTTTGAGAGG TCACCAACGT AATGCCATTG ACTTTCTTGA TGTAGGATAT
    TTGGGGCCAC ACAGTCAAGG ACAGTTTACA GGTGAATTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa51975
    Clone ID Related Accession (Same CDS sequence) XM_031896598.1
    Accession Version XM_031896598.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4767bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product roundabout homolog 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAACATCT GGATAATGCT ATCTTCACTA AGGCAGAGGG CAGCTTCTCC TGAATTCCTG 
    CTGATCCTTA TGCTGTTCGT CAGTGGGGTG ACCCAAAGCA AAATTAGTAG GACCAAGGGG
    AAGCACTGGA AAGGGGGATC CCGTCTCCGT CAAGAAGATT TCCCCCCACG GATAGTTGAG
    CATCCATCGG ATGTCATTGT GTCAAAGGGA GAGCCAACCA CCTTGAACTG CAAAGCGGAA
    GGGCGGCCTA CACCCACCAT AGAGTGGTAC AAAGACTTAG AAAGGGTTGA GACAGACAAG
    GATGACCCCC GCTCACATCG AATGCTACTG CCAAGTGGAT CTTTATTTTT TCTGCGCATT
    ATCCATGGGC GCAAGTTTAA GCCAGACGAA GGAAGTTACG TCTGTGTGGC AAGGAACTAT
    CTCGGGGAGG CTATGAGTCG AAATGCATCT CTGGAAGTGG CATTGCTCCG TGATGACTTC
    CGGCTCAACC CTACAGATGT TGTTGTGGCA GCTGGGGAAC CTGCAATTTT GGAGTGTCAA
    CCCCCCAGAG GACATCCGGA ACCAACTATA TACTGGAAAA AGGACAAAGT TAGAATTGAT
    GATAAAGATG AAAGAATTAG TATTCGAGGT GGAAAGCTGA TGCTCTCCAA TACAAGGAAA
    AGTGATGCAG GCATGTACAC TTGTGTTGGG ACCAACATGG TTGGAGAAAG AGACAGTGAC
    CCAGCAGAGC TAACAGTCTT TGAACGTCCC ACATTCCTCA GAAGGCCAAT TAATCAAGTT
    GTTTTAGAGG ATGAAGCTGT AGAATTTCGA TGTCAGGTCC AGGGGGACCC ACAGCCAACT
    GTCCGGTGGA AAAAGGATGA TGTAGATTTG CCAAGAGGAA GATACGACAT CAAGGATGAC
    TATACTTTGA GAATCAAGAA GGCTTCCAGC AGCGACGAAG GGACCTTTGC TTGTATTTCT
    GAAAACCGGG TTGGTAAAAT AGAAGCTTCT GCCACACTTA CAGTCAGAGT TCGCCCTGTT
    GCTCCTCCCC AGTTTGTGAT CAGACCAAGG GATCAGATTG TAGCACAAGG CCGGACAGTA
    ACATTTGGAT GTGAAGCTAA AGGAAATCCT CAACCAGCTG TTTTTTGGCA AAAAGAAGGA
    AGCCAGAATT TACTTTTTCC AAATCAGCCT CTTCAGCCCA ACAGCAGGTA CTCAGTGTCA
    CCAACTGGAG ATTTAACCAT CACCAGCATT CAGCGCTCAG ACGCAGGCTA CTACATTTGT
    CAGGCACTGA CTGTTGCCGG AAGCATTCTG GCCAAGGCCC AGCTGGAAGT GACTGATGTT
    TTGACAGATA GACCACCGCC AATAATTCTA CAAGGTCCAG CTAATCAAAC TCTTGCCGTG
    GATGGAACAG CAGTGCTGAA ATGTAAAACT ACTGGGGACC CAATGCCAGT CATAAGTTGG
    CTAAAAGAGG GATTTAAGGG ATTCATAGGG AGAGATGCAA GGTTATCCAT TCTAGACCAA
    GGGACATTAC AAATTAAAGG TTTAAAGGTT ACTGATTCTG GCACCTATAC TTGTGTGGCT
    ACTAGCTCTA GTGGTGAGAC AACATGGAGC GCTGTTCTGG AAGTGACAGA ATCTGGTGGT
    GCCATAATTA GCAAGAACTA TGATCTCAAT GACCTTCCTG GATCTCCATC AAAACCTCAG
    GTTACAGATG TCAGTAAGAA CAGTGTTACC CTATCATGGC AACCAGGATC ACCAGGGATT
    CTGCCTGTGA CATCCTACAT CATTGAGGCT TTCAGTCAAT CTGTGAGCAA TAGTTGGCAA
    ACTGTGGCCA ATCACGTGAA GACAACGCTT TACACTGTTA AAGGACTGCG CCCAAACACC
    ATTTATCTCT TTATGGTGAG AGCAATCAAT ACTGAAGGAC TGAGTGACCC AAGTCCAATA
    TCTGATCCAG TGCGTACACA AGATATAAGT CCACCTGTTC AAGGAGTAGA TCACCGGCAA
    GTACAGAAAG AACTTGGGGA TGTGATTGTA CGGCTCCATA ATCCTGTTGT TCTGACACCA
    ACAACAATCC AGGTCACCTG GATGGTCGAC CGTCAATCCC AGTTTATTCA GGGATACAGG
    GTAATGTACC GTCAGACTTC TGGTCTGCAG ACTCCATCTG CGTGGCAGAG CTTAGAGGTC
    AAAGTGCCTG CAGAGCGTAG CGCAGTGCTA ACCAACCTGA AAAAAGGAAT TACTTATGAG
    ATTAAGGTTC GACCTTATTT TAATGAATTT CAAGGAATGG ATAGCGAGTC AAAGTCAGCA
    CGAACAACTG AAGAAGCCCC AAGTGCTCCT CCTCAGTCTG TTACTGTTAT GACAGTGGGA
    AATAACAACA GCACCAGCAT CAGCATCTCA TGGGACCCCC CTCCATCTGA CCACCAGAAT
    GGAATTATCC AGGACTACAG GATCTGGTGC CTGGGTAATG AGACACGTTT CCACATCAAC
    AAGACAGTGG ATGCTGCTAT TCGTTCCGTG GTTATCGGTG GGCTATTCCC TGGAATTCAG
    TACCGCGTTG AAGTCGCAGC CAGTACAAGC GCAGGCACTG GGGTAAAAAG TGCTCCCCAG
    CACATACAGA TAGGAAATCG GAATGAAGTT GTGATCACAG AGAATAACAA CAGCATAACA
    GAGCAAATAA CTGATGTTGT GAAACAGCCG GCATTCATCG CAGGAATTGG TGGTGCCTGC
    TGGGTAATAC TCATGGGATT TAGCATTTGG CTCTACTGGC GAAGAAAGAA GAGAAAGGGT
    CTCAGCAACT ACGCTGTTAC ATTCCAGAGA GGAGATGGAG GACTAATGAG TAATGGAAGT
    CGTCCTGGAC TTTTAAATGC GAATGATCCT AGTTACCCTT GGTTGGCAGA CTCCTGGCCA
    GCTTCCAGCA TGCCGGTGAA TAACAGCAAC AATGGACCAA ACGATCTCGT TAATTTTAGT
    TGTGGAGATG TGCTGCCAAC AGTTCCAGGT CAAGGAGAAA AAACAGCGAC TATGCTTTCT
    GATGGAGCCA TTTATAGCAG CATTGATTTT ACTACAAAAT CTAGCTATAA TTGCCCAGGA
    CAAATTGCAC AAACCACGCC ATATGCTACT ACGCAGATAC TACATTCCAA CAGCATTCAT
    GAGCTGGCAG TTGACTTGCC TGATCCACAG TGGAAATCTT CCATTCAGCA GAAATCTGAT
    CTCACTGGAT TAGGCTATTG CCTTACTGAC CAAGGCAAAG GGAGTAATGC CTTACTTTAC
    ATTCCTGACT ATCGAATGGC TGAGGGATTG TCAAATAGAA TACCCCACAA CCAGTCACAG
    GATTTCAGCA CCACCAGCTC TCACAACAGC TCAGAGAGAA GTGGCAGCCT TTCAGGTGGA
    AAAGACGGAA AAAGGAAGAA AAATAAAAAC AGCTCTAAAA TCTCTAAAAA CAATGGATCC
    AGTTGGGCCA ATGTTCCACT TCCGCCCCCT CCCATCCAAC CACTGCCAGG CACTGAAATA
    GAACATTATG GCCTTGAACA CCAGGAAACA GGCTATGACA CCAATGGATG GTGCCCACCT
    TTGCCAGTCC AGACCTACTT ACATCAAGGA TCAGATGATG AACTAGAAGA AGATGAAGAG
    AGAGTCCCTA CACCCCCTGT ACGTGGGGTG GCATCATCAC CTGCCATCTC ATTTGGACAA
    CAGTCAACAG CAACCCTCAC ACCATCACCA CGTGAAGAAA TGCAACCCAT GCTTCAGGCC
    CACCTGGATG AATTAACAAG AGCTTACCAG TTTGACATTG CAAAGCAATC TTGGCATTTA
    CACAGCAATA ACCAGCCCCC ATTGGCCCCA GTTCCACCAC TTGGATATGT GTCTGGGACA
    TTTATTTCTG ACTTAGAAAA AGACATTCCA GATCAAGAAG AAGATGAAAT TGAAGACGAA
    TCTATAGAAA TAACAAGGCC CCTCCGAGGT TTGGAACACA CTCCCGGTTC TAGTATGGAT
    AATCTCGACA GTTCTGTGAC AGGTTCAATG GTAAATGGAT GGGGTTCTGC ATCTGATGAG
    GATCGGAACT ATTCTAGTCA TAGATCTAGT GTAGGTAGCG CAACAGATGA CTCGATAATT
    GCAAGTGGCA ACTTTGCACA ATCACTGGTT GCAGCAGCAG ATAAAGCTGG TTTCTCACTA
    AATGGAAACA GCCTATCTAT AGCAGGCAAA ATGAACTCCT TTTCTCAGAG ACCCCGGCCT
    GCCAGTCCCT TTTCTACTGA CAGTAACACA AGTTCAGCTG TGAATCATAG TCAGAAGTCA
    CGGCCCACTA AGAAACACAA GGGAGGCAGA ATAGGTCAGC AACCCATGCT TCGCAGAGAA
    GGGATTGCAG ATGATTTGCC TCCCCCACCT GATCCTCCAC CTTGTCAAAG ACAGCAAATC
    ATGCCCAGTC AACGTGGTGG AGGTCCTACA GAAAGAAAAG CCAGTTCTCT TGAGAGGCAC
    ACATCAAATA TGAATGATTT AAAAACCTCA TTAGACCGGC ATGGCAGAAC ATCCTTAGAA
    CGGCAGCGAC AAACACAGGA ATGGCTTGGC TCAACAGAAA GGCAGAACAA GCAGGCATTT
    GGATCTGAAG AGATACTAAT GCCATACAGT AAACCTAGCT TCCCATCTCC AGGAGGGCAC
    AGCTCCTCAG GAACAGTCTC TTCAAAAGAA TCAACTGGAC CCAGAAAAGG GGAGGTTTTG
    AGAGGTCACC AACGTAATGC CATTGACTTT CTTGATGTAG GATATTTGGG GCCACACAGT
    CAAGGACAGT TTACAGGTGA ATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752458.1
    CDS545..5311
    Translation

    Target ORF information:

    RefSeq Version XM_031896598.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896598.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAACATCT GGATAATGCT ATCTTCACTA AGGCAGAGGG CAGCTTCTCC TGAATTCCTG 
    CTGATCCTTA TGCTGTTCGT CAGTGGGGTG ACCCAAAGCA AAATTAGTAG GACCAAGGGG
    AAGCACTGGA AAGGGGGATC CCGTCTCCGT CAAGAAGATT TCCCCCCACG GATAGTTGAG
    CATCCATCGG ATGTCATTGT GTCAAAGGGA GAGCCAACCA CCTTGAACTG CAAAGCGGAA
    GGGCGGCCTA CACCCACCAT AGAGTGGTAC AAAGACTTAG AAAGGGTTGA GACAGACAAG
    GATGACCCCC GCTCACATCG AATGCTACTG CCAAGTGGAT CTTTATTTTT TCTGCGCATT
    ATCCATGGGC GCAAGTTTAA GCCAGACGAA GGAAGTTACG TCTGTGTGGC AAGGAACTAT
    CTCGGGGAGG CTATGAGTCG AAATGCATCT CTGGAAGTGG CATTGCTCCG TGATGACTTC
    CGGCTCAACC CTACAGATGT TGTTGTGGCA GCTGGGGAAC CTGCAATTTT GGAGTGTCAA
    CCCCCCAGAG GACATCCGGA ACCAACTATA TACTGGAAAA AGGACAAAGT TAGAATTGAT
    GATAAAGATG AAAGAATTAG TATTCGAGGT GGAAAGCTGA TGCTCTCCAA TACAAGGAAA
    AGTGATGCAG GCATGTACAC TTGTGTTGGG ACCAACATGG TTGGAGAAAG AGACAGTGAC
    CCAGCAGAGC TAACAGTCTT TGAACGTCCC ACATTCCTCA GAAGGCCAAT TAATCAAGTT
    GTTTTAGAGG ATGAAGCTGT AGAATTTCGA TGTCAGGTCC AGGGGGACCC ACAGCCAACT
    GTCCGGTGGA AAAAGGATGA TGTAGATTTG CCAAGAGGAA GATACGACAT CAAGGATGAC
    TATACTTTGA GAATCAAGAA GGCTTCCAGC AGCGACGAAG GGACCTTTGC TTGTATTTCT
    GAAAACCGGG TTGGTAAAAT AGAAGCTTCT GCCACACTTA CAGTCAGAGT TCGCCCTGTT
    GCTCCTCCCC AGTTTGTGAT CAGACCAAGG GATCAGATTG TAGCACAAGG CCGGACAGTA
    ACATTTGGAT GTGAAGCTAA AGGAAATCCT CAACCAGCTG TTTTTTGGCA AAAAGAAGGA
    AGCCAGAATT TACTTTTTCC AAATCAGCCT CTTCAGCCCA ACAGCAGGTA CTCAGTGTCA
    CCAACTGGAG ATTTAACCAT CACCAGCATT CAGCGCTCAG ACGCAGGCTA CTACATTTGT
    CAGGCACTGA CTGTTGCCGG AAGCATTCTG GCCAAGGCCC AGCTGGAAGT GACTGATGTT
    TTGACAGATA GACCACCGCC AATAATTCTA CAAGGTCCAG CTAATCAAAC TCTTGCCGTG
    GATGGAACAG CAGTGCTGAA ATGTAAAACT ACTGGGGACC CAATGCCAGT CATAAGTTGG
    CTAAAAGAGG GATTTAAGGG ATTCATAGGG AGAGATGCAA GGTTATCCAT TCTAGACCAA
    GGGACATTAC AAATTAAAGG TTTAAAGGTT ACTGATTCTG GCACCTATAC TTGTGTGGCT
    ACTAGCTCTA GTGGTGAGAC AACATGGAGC GCTGTTCTGG AAGTGACAGA ATCTGGTGGT
    GCCATAATTA GCAAGAACTA TGATCTCAAT GACCTTCCTG GATCTCCATC AAAACCTCAG
    GTTACAGATG TCAGTAAGAA CAGTGTTACC CTATCATGGC AACCAGGATC ACCAGGGATT
    CTGCCTGTGA CATCCTACAT CATTGAGGCT TTCAGTCAAT CTGTGAGCAA TAGTTGGCAA
    ACTGTGGCCA ATCACGTGAA GACAACGCTT TACACTGTTA AAGGACTGCG CCCAAACACC
    ATTTATCTCT TTATGGTGAG AGCAATCAAT ACTGAAGGAC TGAGTGACCC AAGTCCAATA
    TCTGATCCAG TGCGTACACA AGATATAAGT CCACCTGTTC AAGGAGTAGA TCACCGGCAA
    GTACAGAAAG AACTTGGGGA TGTGATTGTA CGGCTCCATA ATCCTGTTGT TCTGACACCA
    ACAACAATCC AGGTCACCTG GATGGTCGAC CGTCAATCCC AGTTTATTCA GGGATACAGG
    GTAATGTACC GTCAGACTTC TGGTCTGCAG ACTCCATCTG CGTGGCAGAG CTTAGAGGTC
    AAAGTGCCTG CAGAGCGTAG CGCAGTGCTA ACCAACCTGA AAAAAGGAAT TACTTATGAG
    ATTAAGGTTC GACCTTATTT TAATGAATTT CAAGGAATGG ATAGCGAGTC AAAGTCAGCA
    CGAACAACTG AAGAAGCCCC AAGTGCTCCT CCTCAGTCTG TTACTGTTAT GACAGTGGGA
    AATAACAACA GCACCAGCAT CAGCATCTCA TGGGACCCCC CTCCATCTGA CCACCAGAAT
    GGAATTATCC AGGACTACAG GATCTGGTGC CTGGGTAATG AGACACGTTT CCACATCAAC
    AAGACAGTGG ATGCTGCTAT TCGTTCCGTG GTTATCGGTG GGCTATTCCC TGGAATTCAG
    TACCGCGTTG AAGTCGCAGC CAGTACAAGC GCAGGCACTG GGGTAAAAAG TGCTCCCCAG
    CACATACAGA TAGGAAATCG GAATGAAGTT GTGATCACAG AGAATAACAA CAGCATAACA
    GAGCAAATAA CTGATGTTGT GAAACAGCCG GCATTCATCG CAGGAATTGG TGGTGCCTGC
    TGGGTAATAC TCATGGGATT TAGCATTTGG CTCTACTGGC GAAGAAAGAA GAGAAAGGGT
    CTCAGCAACT ACGCTGTTAC ATTCCAGAGA GGAGATGGAG GACTAATGAG TAATGGAAGT
    CGTCCTGGAC TTTTAAATGC GAATGATCCT AGTTACCCTT GGTTGGCAGA CTCCTGGCCA
    GCTTCCAGCA TGCCGGTGAA TAACAGCAAC AATGGACCAA ACGATCTCGT TAATTTTAGT
    TGTGGAGATG TGCTGCCAAC AGTTCCAGGT CAAGGAGAAA AAACAGCGAC TATGCTTTCT
    GATGGAGCCA TTTATAGCAG CATTGATTTT ACTACAAAAT CTAGCTATAA TTGCCCAGGA
    CAAATTGCAC AAACCACGCC ATATGCTACT ACGCAGATAC TACATTCCAA CAGCATTCAT
    GAGCTGGCAG TTGACTTGCC TGATCCACAG TGGAAATCTT CCATTCAGCA GAAATCTGAT
    CTCACTGGAT TAGGCTATTG CCTTACTGAC CAAGGCAAAG GGAGTAATGC CTTACTTTAC
    ATTCCTGACT ATCGAATGGC TGAGGGATTG TCAAATAGAA TACCCCACAA CCAGTCACAG
    GATTTCAGCA CCACCAGCTC TCACAACAGC TCAGAGAGAA GTGGCAGCCT TTCAGGTGGA
    AAAGACGGAA AAAGGAAGAA AAATAAAAAC AGCTCTAAAA TCTCTAAAAA CAATGGATCC
    AGTTGGGCCA ATGTTCCACT TCCGCCCCCT CCCATCCAAC CACTGCCAGG CACTGAAATA
    GAACATTATG GCCTTGAACA CCAGGAAACA GGCTATGACA CCAATGGATG GTGCCCACCT
    TTGCCAGTCC AGACCTACTT ACATCAAGGA TCAGATGATG AACTAGAAGA AGATGAAGAG
    AGAGTCCCTA CACCCCCTGT ACGTGGGGTG GCATCATCAC CTGCCATCTC ATTTGGACAA
    CAGTCAACAG CAACCCTCAC ACCATCACCA CGTGAAGAAA TGCAACCCAT GCTTCAGGCC
    CACCTGGATG AATTAACAAG AGCTTACCAG TTTGACATTG CAAAGCAATC TTGGCATTTA
    CACAGCAATA ACCAGCCCCC ATTGGCCCCA GTTCCACCAC TTGGATATGT GTCTGGGACA
    TTTATTTCTG ACTTAGAAAA AGACATTCCA GATCAAGAAG AAGATGAAAT TGAAGACGAA
    TCTATAGAAA TAACAAGGCC CCTCCGAGGT TTGGAACACA CTCCCGGTTC TAGTATGGAT
    AATCTCGACA GTTCTGTGAC AGGTTCAATG GTAAATGGAT GGGGTTCTGC ATCTGATGAG
    GATCGGAACT ATTCTAGTCA TAGATCTAGT GTAGGTAGCG CAACAGATGA CTCGATAATT
    GCAAGTGGCA ACTTTGCACA ATCACTGGTT GCAGCAGCAG ATAAAGCTGG TTTCTCACTA
    AATGGAAACA GCCTATCTAT AGCAGGCAAA ATGAACTCCT TTTCTCAGAG ACCCCGGCCT
    GCCAGTCCCT TTTCTACTGA CAGTAACACA AGTTCAGCTG TGAATCATAG TCAGAAGTCA
    CGGCCCACTA AGAAACACAA GGGAGGCAGA ATAGGTCAGC AACCCATGCT TCGCAGAGAA
    GGGATTGCAG ATGATTTGCC TCCCCCACCT GATCCTCCAC CTTGTCAAAG ACAGCAAATC
    ATGCCCAGTC AACGTGGTGG AGGTCCTACA GAAAGAAAAG CCAGTTCTCT TGAGAGGCAC
    ACATCAAATA TGAATGATTT AAAAACCTCA TTAGACCGGC ATGGCAGAAC ATCCTTAGAA
    CGGCAGCGAC AAACACAGGA ATGGCTTGGC TCAACAGAAA GGCAGAACAA GCAGGCATTT
    GGATCTGAAG AGATACTAAT GCCATACAGT AAACCTAGCT TCCCATCTCC AGGAGGGCAC
    AGCTCCTCAG GAACAGTCTC TTCAAAAGAA TCAACTGGAC CCAGAAAAGG GGAGGTTTTG
    AGAGGTCACC AACGTAATGC CATTGACTTT CTTGATGTAG GATATTTGGG GCCACACAGT
    CAAGGACAGT TTACAGGTGA ATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa51976
    Clone ID Related Accession (Same CDS sequence) XM_031896599.1
    Accession Version XM_031896599.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4737bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product roundabout homolog 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAACATCT GGATAATGCT ATCTTCACTA AGGCAGAGGG CAGCTTCTCC TGAATTCCTG 
    CTGATCCTTA TGCTGTTCGT CAGTGGGGTG ACCCAAAGCA AAATTAGTAG GACCAAGGGG
    AAGCACTGGA AAGGGGGATC CCGTCTCCGT CAAGAAGATT TCCCCCCACG GATAGTTGAG
    CATCCATCGG ATGTCATTGT GTCAAAGGGA GAGCCAACCA CCTTGAACTG CAAAGCGGAA
    GGGCGGCCTA CACCCACCAT AGAGTGGTAC AAAGACTTAG AAAGGGTTGA GACAGACAAG
    GATGACCCCC GCTCACATCG AATGCTACTG CCAAGTGGAT CTTTATTTTT TCTGCGCATT
    ATCCATGGGC GCAAGTTTAA GCCAGACGAA GGAAGTTACG TCTGTGTGGC AAGGAACTAT
    CTCGGGGAGG CTATGAGTCG AAATGCATCT CTGGAAGTGG CATTGCTCCG TGATGACTTC
    CGGCTCAACC CTACAGATGT TGTTGTGGCA GCTGGGGAAC CTGCAATTTT GGAGTGTCAA
    CCCCCCAGAG GACATCCGGA ACCAACTATA TACTGGAAAA AGGACAAAGT TAGAATTGAT
    GATAAAGATG AAAGAATTAG TATTCGAGGT GGAAAGCTGA TGCTCTCCAA TACAAGGAAA
    AGTGATGCAG GCATGTACAC TTGTGTTGGG ACCAACATGG TTGGAGAAAG AGACAGTGAC
    CCAGCAGAGC TAACAGTCTT TGAACGTCCC ACATTCCTCA GAAGGCCAAT TAATCAAGTT
    GTTTTAGAGG ATGAAGCTGT AGAATTTCGA TGTCAGGTCC AGGGGGACCC ACAGCCAACT
    GTCCGGTGGA AAAAGGATGA TGTAGATTTG CCAAGAGGAA GATACGACAT CAAGGATGAC
    TATACTTTGA GAATCAAGAA GGCTTCCAGC AGCGACGAAG GGACCTTTGC TTGTATTTCT
    GAAAACCGGG TTGGTAAAAT AGAAGCTTCT GCCACACTTA CAGTCAGAGT TCGCCCTGTT
    GCTCCTCCCC AGTTTGTGAT CAGACCAAGG GATCAGATTG TAGCACAAGG CCGGACAGTA
    ACATTTGGAT GTGAAGCTAA AGGAAATCCT CAACCAGCTG TTTTTTGGCA AAAAGAAGGA
    AGCCAGAATT TACTTTTTCC AAATCAGCCT CTTCAGCCCA ACAGCAGGTA CTCAGTGTCA
    CCAACTGGAG ATTTAACCAT CACCAGCATT CAGCGCTCAG ACGCAGGCTA CTACATTTGT
    CAGGCACTGA CTGTTGCCGG AAGCATTCTG GCCAAGGCCC AGCTGGAAGT GACTGATGTT
    TTGACAGATA GACCACCGCC AATAATTCTA CAAGGTCCAG CTAATCAAAC TCTTGCCGTG
    GATGGAACAG CAGTGCTGAA ATGTAAAACT ACTGGGGACC CAATGCCAGT CATAAGTTGG
    CTAAAAGAGG GATTTAAGGG ATTCATAGGG AGAGATGCAA GGTTATCCAT TCTAGACCAA
    GGGACATTAC AAATTAAAGG TTTAAAGGTT ACTGATTCTG GCACCTATAC TTGTGTGGCT
    ACTAGCTCTA GTGGTGAGAC AACATGGAGC GCTGTTCTGG AAGTGACAGA ATCTGGTGGT
    GCCATAATTA GCAAGAACTA TGATCTCAAT GACCTTCCTG GATCTCCATC AAAACCTCAG
    GTTACAGATG TCAGTAAGAA CAGTGTTACC CTATCATGGC AACCAGGATC ACCAGGGATT
    CTGCCTGTGA CATCCTACAT CATTGAGGCT TTCAGTCAAT CTGTGAGCAA TAGTTGGCAA
    ACTGTGGCCA ATCACGTGAA GACAACGCTT TACACTGTTA AAGGACTGCG CCCAAACACC
    ATTTATCTCT TTATGGTGAG AGCAATCAAT ACTGAAGGAC TGAGTGACCC AAGTCCAATA
    TCTGATCCAG TGCGTACACA AGATATAAGT CCACCTGTTC AAGGAGTAGA TCACCGGCAA
    GTACAGAAAG AACTTGGGGA TGTGATTGTA CGGCTCCATA ATCCTGTTGT TCTGACACCA
    ACAACAATCC AGGTCACCTG GATGGTCGAC CGTCAATCCC AGTTTATTCA GGGATACAGG
    GTAATGTACC GTCAGACTTC TGGTCTGCAG ACTCCATCTG CGTGGCAGAG CTTAGAGGTC
    AAAGTGCCTG CAGAGCGTAG CGCAGTGCTA ACCAACCTGA AAAAAGGAAT TACTTATGAG
    ATTAAGGTTC GACCTTATTT TAATGAATTT CAAGGAATGG ATAGCGAGTC AAAGTCAGCA
    CGAACAACTG AAGAAGCCCC AAGTGCTCCT CCTCAGTCTG TTACTGTTAT GACAGTGGGA
    AATAACAACA GCACCAGCAT CAGCATCTCA TGGGACCCCC CTCCATCTGA CCACCAGAAT
    GGAATTATCC AGGACTACAG GATCTGGTGC CTGGGTAATG AGACACGTTT CCACATCAAC
    AAGACAGTGG ATGCTGCTAT TCGTTCCGTG GTTATCGGTG GGCTATTCCC TGGAATTCAG
    TACCGCGTTG AAGTCGCAGC CAGTACAAGC GCAGGCACTG GGGTAAAAAG TGCTCCCCAG
    CACATACAGA TAGGAAATCG GAATGAAGTT GTGATCACAG AGAATAACAA CAGCATAACA
    GAGCAAATAA CTGATGTTGT GAAACAGCCG GCATTCATCG CAGGAATTGG TGGTGCCTGC
    TGGGTAATAC TCATGGGATT TAGCATTTGG CTCTACTGGC GAAGAAAGAA GAGAAAGGGT
    CTCAGCAACT ACGCTGTTCA GTCTTTCACC TTTACCCCTG CAGATTCCCC AGATACAACC
    ATGCACCACA AGGTTGCACT TGGCACAAAG AAGGGGAGCT GGCACAAAAC AGTTACATTC
    CAGAGAGGAG ATGGAGGACT AATGAGTAAT GGAAGTCGTC CTGGACTTTT AAATGCGAAT
    GATCCTAGTT ACCCTTGGTT GGCAGACTCC TGGCCAGCTT CCAGCATGCC GGTGAATAAC
    AGCAACAATG GACCAAACGA TCTCGTTAAT TTTAGTTGTG GAGATGTGCT GCCAACAGTT
    CCAGGTCAAG GAGAAAAAAC AGCGACTATG CTTTCTGATG GAGCCATTTA TAGCAGCATT
    GATTTTACTA CAAAATCTAG CTATAATTGC CCAGGACAAA TTGCACAAAC CACGCCATAT
    GCTACTACGC AGATACTACA TTCCAACAGC ATTCATGAGC TGGCAGTTGA CTTGCCTGAT
    CCACAGTGGA AATCTTCCAT TCAGCAGAAA TCTGATCTCA CTGGATTAGG CTATTGCCTT
    ACTGACCAAG GCAAAGGGAG TAATGGTGGA AAAGACGGAA AAAGGAAGAA AAATAAAAAC
    AGCTCTAAAA TCTCTAAAAA CAATGGATCC AGTTGGGCCA ATGTTCCACT TCCGCCCCCT
    CCCATCCAAC CACTGCCAGG CACTGAAATA GAACATTATG GCCTTGAACA CCAGGAAACA
    GGCTATGACA CCAATGGATG GTGCCCACCT TTGCCAGTCC AGACCTACTT ACATCAAGGA
    TCAGATGATG AACTAGAAGA AGATGAAGAG AGAGTCCCTA CACCCCCTGT ACGTGGGGTG
    GCATCATCAC CTGCCATCTC ATTTGGACAA CAGTCAACAG CAACCCTCAC ACCATCACCA
    CGTGAAGAAA TGCAACCCAT GCTTCAGGCC CACCTGGATG AATTAACAAG AGCTTACCAG
    TTTGACATTG CAAAGCAATC TTGGCATTTA CACAGCAATA ACCAGCCCCC ATTGGCCCCA
    GTTCCACCAC TTGGATATGT GTCTGGGACA TTTATTTCTG ACTTAGAAAA AGACATTCCA
    GATCAAGAAG AAGATGAAAT TGAAGACGAA TCTATAGAAA TAACAAGGCC CCTCCGAGGT
    TTGGAACACA CTCCCGGTTC TAGTATGGAT AATCTCGACA GTTCTGTGAC AGGTTCAATG
    GTAAATGGAT GGGGTTCTGC ATCTGATGAG GATCGGAACT ATTCTAGTCA TAGATCTAGT
    GTAGGTAGCG CAACAGATGA CTCGATAATT GCAAGTGGCA ACTTTGCACA ATCACTGGTT
    GCAGCAGCAG ATAAAGCTGG TTTCTCACTA AATGGAAACA GCCTATCTAT AGCAGGCAAA
    ATGAACTCCT TTTCTCAGAG ACCCCGGCCT GCCAGTCCCT TTTCTACTGA CAGTAACACA
    AGTTCAGCTG TGAATCATAG TCAGAAGTCA CGGCCCACTA AGAAACACAA GGGAGGCAGA
    ATAGGTCAGC AACCCATGCT TCGCAGAGAA GGGATTGCAG ATGATTTGCC TCCCCCACCT
    GATCCTCCAC CTTGTCAAAG ACAGCAAATC ATGCCCAGTC AACGTGGTGG AGGTCCTACA
    GAAAGAAAAG CCAGTTCTCT TGAGAGGCAC ACATCAAATA TGAATGATTT AAAAACCTCA
    TTAGACCGGC ATGGCAGAAC ATCCTTAGAA CGGCAGCGAC AAACACAGGA ATGGCTTGGC
    TCAACAGAAA GGCAGAACAA GCAGGCATTT GGATCTGAAG AGATACTAAT GCCATACAGT
    AAACCTAGCT TCCCATCTCC AGGAGGGCAC AGCTCCTCAG GAACAGTCTC TTCAAAAGAA
    TCAACTGGAC CCAGAAAAGG GGAGGTTTTG AGAGGTCACC AACGTAATGC CATTGACTTT
    CTTGATGTAG GATATTTGGG GCCACACAGT CAAGGACAGT TTACAGGTGA ATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752459.1
    CDS547..5283
    Translation

    Target ORF information:

    RefSeq Version XM_031896599.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896599.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAACATCT GGATAATGCT ATCTTCACTA AGGCAGAGGG CAGCTTCTCC TGAATTCCTG 
    CTGATCCTTA TGCTGTTCGT CAGTGGGGTG ACCCAAAGCA AAATTAGTAG GACCAAGGGG
    AAGCACTGGA AAGGGGGATC CCGTCTCCGT CAAGAAGATT TCCCCCCACG GATAGTTGAG
    CATCCATCGG ATGTCATTGT GTCAAAGGGA GAGCCAACCA CCTTGAACTG CAAAGCGGAA
    GGGCGGCCTA CACCCACCAT AGAGTGGTAC AAAGACTTAG AAAGGGTTGA GACAGACAAG
    GATGACCCCC GCTCACATCG AATGCTACTG CCAAGTGGAT CTTTATTTTT TCTGCGCATT
    ATCCATGGGC GCAAGTTTAA GCCAGACGAA GGAAGTTACG TCTGTGTGGC AAGGAACTAT
    CTCGGGGAGG CTATGAGTCG AAATGCATCT CTGGAAGTGG CATTGCTCCG TGATGACTTC
    CGGCTCAACC CTACAGATGT TGTTGTGGCA GCTGGGGAAC CTGCAATTTT GGAGTGTCAA
    CCCCCCAGAG GACATCCGGA ACCAACTATA TACTGGAAAA AGGACAAAGT TAGAATTGAT
    GATAAAGATG AAAGAATTAG TATTCGAGGT GGAAAGCTGA TGCTCTCCAA TACAAGGAAA
    AGTGATGCAG GCATGTACAC TTGTGTTGGG ACCAACATGG TTGGAGAAAG AGACAGTGAC
    CCAGCAGAGC TAACAGTCTT TGAACGTCCC ACATTCCTCA GAAGGCCAAT TAATCAAGTT
    GTTTTAGAGG ATGAAGCTGT AGAATTTCGA TGTCAGGTCC AGGGGGACCC ACAGCCAACT
    GTCCGGTGGA AAAAGGATGA TGTAGATTTG CCAAGAGGAA GATACGACAT CAAGGATGAC
    TATACTTTGA GAATCAAGAA GGCTTCCAGC AGCGACGAAG GGACCTTTGC TTGTATTTCT
    GAAAACCGGG TTGGTAAAAT AGAAGCTTCT GCCACACTTA CAGTCAGAGT TCGCCCTGTT
    GCTCCTCCCC AGTTTGTGAT CAGACCAAGG GATCAGATTG TAGCACAAGG CCGGACAGTA
    ACATTTGGAT GTGAAGCTAA AGGAAATCCT CAACCAGCTG TTTTTTGGCA AAAAGAAGGA
    AGCCAGAATT TACTTTTTCC AAATCAGCCT CTTCAGCCCA ACAGCAGGTA CTCAGTGTCA
    CCAACTGGAG ATTTAACCAT CACCAGCATT CAGCGCTCAG ACGCAGGCTA CTACATTTGT
    CAGGCACTGA CTGTTGCCGG AAGCATTCTG GCCAAGGCCC AGCTGGAAGT GACTGATGTT
    TTGACAGATA GACCACCGCC AATAATTCTA CAAGGTCCAG CTAATCAAAC TCTTGCCGTG
    GATGGAACAG CAGTGCTGAA ATGTAAAACT ACTGGGGACC CAATGCCAGT CATAAGTTGG
    CTAAAAGAGG GATTTAAGGG ATTCATAGGG AGAGATGCAA GGTTATCCAT TCTAGACCAA
    GGGACATTAC AAATTAAAGG TTTAAAGGTT ACTGATTCTG GCACCTATAC TTGTGTGGCT
    ACTAGCTCTA GTGGTGAGAC AACATGGAGC GCTGTTCTGG AAGTGACAGA ATCTGGTGGT
    GCCATAATTA GCAAGAACTA TGATCTCAAT GACCTTCCTG GATCTCCATC AAAACCTCAG
    GTTACAGATG TCAGTAAGAA CAGTGTTACC CTATCATGGC AACCAGGATC ACCAGGGATT
    CTGCCTGTGA CATCCTACAT CATTGAGGCT TTCAGTCAAT CTGTGAGCAA TAGTTGGCAA
    ACTGTGGCCA ATCACGTGAA GACAACGCTT TACACTGTTA AAGGACTGCG CCCAAACACC
    ATTTATCTCT TTATGGTGAG AGCAATCAAT ACTGAAGGAC TGAGTGACCC AAGTCCAATA
    TCTGATCCAG TGCGTACACA AGATATAAGT CCACCTGTTC AAGGAGTAGA TCACCGGCAA
    GTACAGAAAG AACTTGGGGA TGTGATTGTA CGGCTCCATA ATCCTGTTGT TCTGACACCA
    ACAACAATCC AGGTCACCTG GATGGTCGAC CGTCAATCCC AGTTTATTCA GGGATACAGG
    GTAATGTACC GTCAGACTTC TGGTCTGCAG ACTCCATCTG CGTGGCAGAG CTTAGAGGTC
    AAAGTGCCTG CAGAGCGTAG CGCAGTGCTA ACCAACCTGA AAAAAGGAAT TACTTATGAG
    ATTAAGGTTC GACCTTATTT TAATGAATTT CAAGGAATGG ATAGCGAGTC AAAGTCAGCA
    CGAACAACTG AAGAAGCCCC AAGTGCTCCT CCTCAGTCTG TTACTGTTAT GACAGTGGGA
    AATAACAACA GCACCAGCAT CAGCATCTCA TGGGACCCCC CTCCATCTGA CCACCAGAAT
    GGAATTATCC AGGACTACAG GATCTGGTGC CTGGGTAATG AGACACGTTT CCACATCAAC
    AAGACAGTGG ATGCTGCTAT TCGTTCCGTG GTTATCGGTG GGCTATTCCC TGGAATTCAG
    TACCGCGTTG AAGTCGCAGC CAGTACAAGC GCAGGCACTG GGGTAAAAAG TGCTCCCCAG
    CACATACAGA TAGGAAATCG GAATGAAGTT GTGATCACAG AGAATAACAA CAGCATAACA
    GAGCAAATAA CTGATGTTGT GAAACAGCCG GCATTCATCG CAGGAATTGG TGGTGCCTGC
    TGGGTAATAC TCATGGGATT TAGCATTTGG CTCTACTGGC GAAGAAAGAA GAGAAAGGGT
    CTCAGCAACT ACGCTGTTCA GTCTTTCACC TTTACCCCTG CAGATTCCCC AGATACAACC
    ATGCACCACA AGGTTGCACT TGGCACAAAG AAGGGGAGCT GGCACAAAAC AGTTACATTC
    CAGAGAGGAG ATGGAGGACT AATGAGTAAT GGAAGTCGTC CTGGACTTTT AAATGCGAAT
    GATCCTAGTT ACCCTTGGTT GGCAGACTCC TGGCCAGCTT CCAGCATGCC GGTGAATAAC
    AGCAACAATG GACCAAACGA TCTCGTTAAT TTTAGTTGTG GAGATGTGCT GCCAACAGTT
    CCAGGTCAAG GAGAAAAAAC AGCGACTATG CTTTCTGATG GAGCCATTTA TAGCAGCATT
    GATTTTACTA CAAAATCTAG CTATAATTGC CCAGGACAAA TTGCACAAAC CACGCCATAT
    GCTACTACGC AGATACTACA TTCCAACAGC ATTCATGAGC TGGCAGTTGA CTTGCCTGAT
    CCACAGTGGA AATCTTCCAT TCAGCAGAAA TCTGATCTCA CTGGATTAGG CTATTGCCTT
    ACTGACCAAG GCAAAGGGAG TAATGGTGGA AAAGACGGAA AAAGGAAGAA AAATAAAAAC
    AGCTCTAAAA TCTCTAAAAA CAATGGATCC AGTTGGGCCA ATGTTCCACT TCCGCCCCCT
    CCCATCCAAC CACTGCCAGG CACTGAAATA GAACATTATG GCCTTGAACA CCAGGAAACA
    GGCTATGACA CCAATGGATG GTGCCCACCT TTGCCAGTCC AGACCTACTT ACATCAAGGA
    TCAGATGATG AACTAGAAGA AGATGAAGAG AGAGTCCCTA CACCCCCTGT ACGTGGGGTG
    GCATCATCAC CTGCCATCTC ATTTGGACAA CAGTCAACAG CAACCCTCAC ACCATCACCA
    CGTGAAGAAA TGCAACCCAT GCTTCAGGCC CACCTGGATG AATTAACAAG AGCTTACCAG
    TTTGACATTG CAAAGCAATC TTGGCATTTA CACAGCAATA ACCAGCCCCC ATTGGCCCCA
    GTTCCACCAC TTGGATATGT GTCTGGGACA TTTATTTCTG ACTTAGAAAA AGACATTCCA
    GATCAAGAAG AAGATGAAAT TGAAGACGAA TCTATAGAAA TAACAAGGCC CCTCCGAGGT
    TTGGAACACA CTCCCGGTTC TAGTATGGAT AATCTCGACA GTTCTGTGAC AGGTTCAATG
    GTAAATGGAT GGGGTTCTGC ATCTGATGAG GATCGGAACT ATTCTAGTCA TAGATCTAGT
    GTAGGTAGCG CAACAGATGA CTCGATAATT GCAAGTGGCA ACTTTGCACA ATCACTGGTT
    GCAGCAGCAG ATAAAGCTGG TTTCTCACTA AATGGAAACA GCCTATCTAT AGCAGGCAAA
    ATGAACTCCT TTTCTCAGAG ACCCCGGCCT GCCAGTCCCT TTTCTACTGA CAGTAACACA
    AGTTCAGCTG TGAATCATAG TCAGAAGTCA CGGCCCACTA AGAAACACAA GGGAGGCAGA
    ATAGGTCAGC AACCCATGCT TCGCAGAGAA GGGATTGCAG ATGATTTGCC TCCCCCACCT
    GATCCTCCAC CTTGTCAAAG ACAGCAAATC ATGCCCAGTC AACGTGGTGG AGGTCCTACA
    GAAAGAAAAG CCAGTTCTCT TGAGAGGCAC ACATCAAATA TGAATGATTT AAAAACCTCA
    TTAGACCGGC ATGGCAGAAC ATCCTTAGAA CGGCAGCGAC AAACACAGGA ATGGCTTGGC
    TCAACAGAAA GGCAGAACAA GCAGGCATTT GGATCTGAAG AGATACTAAT GCCATACAGT
    AAACCTAGCT TCCCATCTCC AGGAGGGCAC AGCTCCTCAG GAACAGTCTC TTCAAAAGAA
    TCAACTGGAC CCAGAAAAGG GGAGGTTTTG AGAGGTCACC AACGTAATGC CATTGACTTT
    CTTGATGTAG GATATTTGGG GCCACACAGT CAAGGACAGT TTACAGGTGA ATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa51977
    Clone ID Related Accession (Same CDS sequence) XM_031896600.1
    Accession Version XM_031896600.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4680bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product roundabout homolog 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAACATCT GGATAATGCT ATCTTCACTA AGGCAGAGGG CAGCTTCTCC TGAATTCCTG 
    CTGATCCTTA TGCTGTTCGT CAGTGGGGTG ACCCAAAGCA AAATTAGTAG GACCAAGGGG
    AAGCACTGGA AAGGGGGATC CCGTCTCCGT CAAGAAGATT TCCCCCCACG GATAGTTGAG
    CATCCATCGG ATGTCATTGT GTCAAAGGGA GAGCCAACCA CCTTGAACTG CAAAGCGGAA
    GGGCGGCCTA CACCCACCAT AGAGTGGTAC AAAGACTTAG AAAGGGTTGA GACAGACAAG
    GATGACCCCC GCTCACATCG AATGCTACTG CCAAGTGGAT CTTTATTTTT TCTGCGCATT
    ATCCATGGGC GCAAGTTTAA GCCAGACGAA GGAAGTTACG TCTGTGTGGC AAGGAACTAT
    CTCGGGGAGG CTATGAGTCG AAATGCATCT CTGGAAGTGG CATTGCTCCG TGATGACTTC
    CGGCTCAACC CTACAGATGT TGTTGTGGCA GCTGGGGAAC CTGCAATTTT GGAGTGTCAA
    CCCCCCAGAG GACATCCGGA ACCAACTATA TACTGGAAAA AGGACAAAGT TAGAATTGAT
    GATAAAGATG AAAGAATTAG TATTCGAGGT GGAAAGCTGA TGCTCTCCAA TACAAGGAAA
    AGTGATGCAG GCATGTACAC TTGTGTTGGG ACCAACATGG TTGGAGAAAG AGACAGTGAC
    CCAGCAGAGC TAACAGTCTT TGAACGTCCC ACATTCCTCA GAAGGCCAAT TAATCAAGTT
    GTTTTAGAGG ATGAAGCTGT AGAATTTCGA TGTCAGGTCC AGGGGGACCC ACAGCCAACT
    GTCCGGTGGA AAAAGGATGA TGTAGATTTG CCAAGAGGAA GATACGACAT CAAGGATGAC
    TATACTTTGA GAATCAAGAA GGCTTCCAGC AGCGACGAAG GGACCTTTGC TTGTATTTCT
    GAAAACCGGG TTGGTAAAAT AGAAGCTTCT GCCACACTTA CAGTCAGAGT TCGCCCTGTT
    GCTCCTCCCC AGTTTGTGAT CAGACCAAGG GATCAGATTG TAGCACAAGG CCGGACAGTA
    ACATTTGGAT GTGAAGCTAA AGGAAATCCT CAACCAGCTG TTTTTTGGCA AAAAGAAGGA
    AGCCAGAATT TACTTTTTCC AAATCAGCCT CTTCAGCCCA ACAGCAGGTA CTCAGTGTCA
    CCAACTGGAG ATTTAACCAT CACCAGCATT CAGCGCTCAG ACGCAGGCTA CTACATTTGT
    CAGGCACTGA CTGTTGCCGG AAGCATTCTG GCCAAGGCCC AGCTGGAAGT GACTGATGTT
    TTGACAGATA GACCACCGCC AATAATTCTA CAAGGTCCAG CTAATCAAAC TCTTGCCGTG
    GATGGAACAG CAGTGCTGAA ATGTAAAACT ACTGGGGACC CAATGCCAGT CATAAGTTGG
    CTAAAAGAGG GATTTAAGGG ATTCATAGGG AGAGATGCAA GGTTATCCAT TCTAGACCAA
    GGGACATTAC AAATTAAAGG TTTAAAGGTT ACTGATTCTG GCACCTATAC TTGTGTGGCT
    ACTAGCTCTA GTGGTGAGAC AACATGGAGC GCTGTTCTGG AAGTGACAGA ATCTGGTGGT
    GCCATAATTA GCAAGAACTA TGATCTCAAT GACCTTCCTG GATCTCCATC AAAACCTCAG
    GTTACAGATG TCAGTAAGAA CAGTGTTACC CTATCATGGC AACCAGGATC ACCAGGGATT
    CTGCCTGTGA CATCCTACAT CATTGAGGCT TTCAGTCAAT CTGTGAGCAA TAGTTGGCAA
    ACTGTGGCCA ATCACGTGAA GACAACGCTT TACACTGTTA AAGGACTGCG CCCAAACACC
    ATTTATCTCT TTATGGTGAG AGCAATCAAT ACTGAAGGAC TGAGTGACCC AAGTCCAATA
    TCTGATCCAG TGCGTACACA AGATATAAGT CCACCTGTTC AAGGAGTAGA TCACCGGCAA
    GTACAGAAAG AACTTGGGGA TGTGATTGTA CGGCTCCATA ATCCTGTTGT TCTGACACCA
    ACAACAATCC AGGTCACCTG GATGGTCGAC CGTCAATCCC AGTTTATTCA GGGATACAGG
    GTAATGTACC GTCAGACTTC TGGTCTGCAG ACTCCATCTG CGTGGCAGAG CTTAGAGGTC
    AAAGTGCCTG CAGAGCGTAG CGCAGTGCTA ACCAACCTGA AAAAAGGAAT TACTTATGAG
    ATTAAGGTTC GACCTTATTT TAATGAATTT CAAGGAATGG ATAGCGAGTC AAAGTCAGCA
    CGAACAACTG AAGAAGCCCC AAGTGCTCCT CCTCAGTCTG TTACTGTTAT GACAGTGGGA
    AATAACAACA GCACCAGCAT CAGCATCTCA TGGGACCCCC CTCCATCTGA CCACCAGAAT
    GGAATTATCC AGGACTACAG GATCTGGTGC CTGGGTAATG AGACACGTTT CCACATCAAC
    AAGACAGTGG ATGCTGCTAT TCGTTCCGTG GTTATCGGTG GGCTATTCCC TGGAATTCAG
    TACCGCGTTG AAGTCGCAGC CAGTACAAGC GCAGGCACTG GGGTAAAAAG TGCTCCCCAG
    CACATACAGA TAGGAAATCG GAATGAAGTT GTGATCACAG AGAATAACAA CAGCATAACA
    GAGCAAATAA CTGATGTTGT GAAACAGCCG GCATTCATCG CAGGAATTGG TGGTGCCTGC
    TGGGTAATAC TCATGGGATT TAGCATTTGG CTCTACTGGC GAAGAAAGAA GAGAAAGGGT
    CTCAGCAACT ACGCTGTTCA GTCTTTCACC TTTACCCCTG CAGATTCCCC AGATACAACC
    ATGCACCACA AGGTTGCACT TGGCACAAAG AAGGGGAGCT GGCACAAAAC AGTTACATTC
    CAGAGAGGAG ATGGAGGACT AATGAGTAAT GGAAGTCGTC CTGGACTTTT AAATGCGAAT
    GATCCTAGTT ACCCTTGGTT GGCAGACTCC TGGCCAGCTT CCAGCATGCC GGTGAATAAC
    AGCAACAATG GACCAAACGA TCTCGTTAAT TTTAGTTGTG GAGATGTGCT GCCAACAGTT
    CCAGGTCAAG GAGAAAAAAC AGCGACTATG CTTTCTGATG GAGCCATTTA TAGCAGCATT
    GATTTTACTA CAAAATCTAG CTATAATTGC CCAGGACAAA TTGCACAAAC CACGCCATAT
    GCTACTACGC AGATACTACA TTCCAACAGC ATTCATGAGC TGGCAGTTGA CTTGCCTGAT
    CCACAGTGGA AATCTTCCAT TCAGCAGAAA TCTGATCTCA CTGGATTAGG CTATTGCCTT
    ACTGACCAAG GCAAAGGGAG TAATGCCTTA CTTTACATTC CTGACTATCG AATGGCTGAG
    GGATTGTCAA ATAGAATACC CCACAACCAG TCACAGGATT TCAGCACCAC CAGCTCTCAC
    AACAGCTCAG AGAGAAGTGG CAGCCTTTCA GGTGGAAAAG ACGGAAAAAG GAAGAAAAAT
    AAAAACAGCT CTAAAATCTC TAAAAACAAT GGATCCAGTT GGGCCAATGT TCCACTTCCG
    CCCCCTCCCA TCCAACCACT GCCAGGCACT GAAATAGAAC ATTATGGCCT TGAACACCAG
    GAAACAGGCT ATGACACCAA TGGATGGTGC CCACCTTTGC CAGTCCAGAC CTACTTACAT
    CAAGGATCAG ATGATGAACT AGAAGAAGAT GAAGAGAGAG TCCCTACACC CCCTGTACGT
    GGGGTGGCAT CATCACCTGC CATCTCATTT GGACAACAGT CAACAGCAAC CCTCACACCA
    TCACCACGTG AAGAAATGCA ACCCATGCTT CAGGCCCACC TGGATGAATT AACAAGAGCT
    TACCAGTTTG ACATTGCAAA GCAATCTTGG CATTTACACA GCAATAACCA GCCCCCATTG
    GCCCCAGTTC CACCACTTGG ATATGTGTCT GGGACATTTA TTTCTGACTT AGAAAAAGAC
    ATTCCAGATC AAGAAGAAGA TGAAATTGAA GACGAATCTA TAGAAATAAC AAGGCCCCTC
    CGAGGTTTGG AACACACTCC CGGTTCTAGT ATGGATAATC TCGACAGTTC TGTGACAGGC
    AAAATGAACT CCTTTTCTCA GAGACCCCGG CCTGCCAGTC CCTTTTCTAC TGACAGTAAC
    ACAAGTTCAG CTGTGAATCA TAGTCAGAAG TCACGGCCCA CTAAGAAACA CAAGGGAGGC
    AGAATAGGTC AGCAACCCAT GCTTCGCAGA GAAGGGATTG CAGATGATTT GCCTCCCCCA
    CCTGATCCTC CACCTTGTCA AAGACAGCAA ATCATGCCCA GTCAACGTGG TGGAGGTCCT
    ACAGAAAGAA AAGCCAGTTC TCTTGAGAGG CACACATCAA ATATGAATGA TTTAAAAACC
    TCATTAGACC GGCATGGCAG AACATCCTTA GAACGGCAGC GACAAACACA GGAATGGCTT
    GGCTCAACAG AAAGGCAGAA CAAGCAGGCA TTTGGATCTG AAGAGATACT AATGCCATAC
    AGTAAACCTA GCTTCCCATC TCCAGGAGGG CACAGCTCCT CAGGAACAGT CTCTTCAAAA
    GAATCAACTG GACCCAGAAA AGGGGAGGTT TTGAGAGGTC ACCAACGTAA TGCCATTGAC
    TTTCTTGATG TAGGATATTT GGGGCCACAC AGTCAAGGAC AGTTTACAGG TGAATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752460.1
    CDS543..5222
    Translation

    Target ORF information:

    RefSeq Version XM_031896600.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896600.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAACATCT GGATAATGCT ATCTTCACTA AGGCAGAGGG CAGCTTCTCC TGAATTCCTG 
    CTGATCCTTA TGCTGTTCGT CAGTGGGGTG ACCCAAAGCA AAATTAGTAG GACCAAGGGG
    AAGCACTGGA AAGGGGGATC CCGTCTCCGT CAAGAAGATT TCCCCCCACG GATAGTTGAG
    CATCCATCGG ATGTCATTGT GTCAAAGGGA GAGCCAACCA CCTTGAACTG CAAAGCGGAA
    GGGCGGCCTA CACCCACCAT AGAGTGGTAC AAAGACTTAG AAAGGGTTGA GACAGACAAG
    GATGACCCCC GCTCACATCG AATGCTACTG CCAAGTGGAT CTTTATTTTT TCTGCGCATT
    ATCCATGGGC GCAAGTTTAA GCCAGACGAA GGAAGTTACG TCTGTGTGGC AAGGAACTAT
    CTCGGGGAGG CTATGAGTCG AAATGCATCT CTGGAAGTGG CATTGCTCCG TGATGACTTC
    CGGCTCAACC CTACAGATGT TGTTGTGGCA GCTGGGGAAC CTGCAATTTT GGAGTGTCAA
    CCCCCCAGAG GACATCCGGA ACCAACTATA TACTGGAAAA AGGACAAAGT TAGAATTGAT
    GATAAAGATG AAAGAATTAG TATTCGAGGT GGAAAGCTGA TGCTCTCCAA TACAAGGAAA
    AGTGATGCAG GCATGTACAC TTGTGTTGGG ACCAACATGG TTGGAGAAAG AGACAGTGAC
    CCAGCAGAGC TAACAGTCTT TGAACGTCCC ACATTCCTCA GAAGGCCAAT TAATCAAGTT
    GTTTTAGAGG ATGAAGCTGT AGAATTTCGA TGTCAGGTCC AGGGGGACCC ACAGCCAACT
    GTCCGGTGGA AAAAGGATGA TGTAGATTTG CCAAGAGGAA GATACGACAT CAAGGATGAC
    TATACTTTGA GAATCAAGAA GGCTTCCAGC AGCGACGAAG GGACCTTTGC TTGTATTTCT
    GAAAACCGGG TTGGTAAAAT AGAAGCTTCT GCCACACTTA CAGTCAGAGT TCGCCCTGTT
    GCTCCTCCCC AGTTTGTGAT CAGACCAAGG GATCAGATTG TAGCACAAGG CCGGACAGTA
    ACATTTGGAT GTGAAGCTAA AGGAAATCCT CAACCAGCTG TTTTTTGGCA AAAAGAAGGA
    AGCCAGAATT TACTTTTTCC AAATCAGCCT CTTCAGCCCA ACAGCAGGTA CTCAGTGTCA
    CCAACTGGAG ATTTAACCAT CACCAGCATT CAGCGCTCAG ACGCAGGCTA CTACATTTGT
    CAGGCACTGA CTGTTGCCGG AAGCATTCTG GCCAAGGCCC AGCTGGAAGT GACTGATGTT
    TTGACAGATA GACCACCGCC AATAATTCTA CAAGGTCCAG CTAATCAAAC TCTTGCCGTG
    GATGGAACAG CAGTGCTGAA ATGTAAAACT ACTGGGGACC CAATGCCAGT CATAAGTTGG
    CTAAAAGAGG GATTTAAGGG ATTCATAGGG AGAGATGCAA GGTTATCCAT TCTAGACCAA
    GGGACATTAC AAATTAAAGG TTTAAAGGTT ACTGATTCTG GCACCTATAC TTGTGTGGCT
    ACTAGCTCTA GTGGTGAGAC AACATGGAGC GCTGTTCTGG AAGTGACAGA ATCTGGTGGT
    GCCATAATTA GCAAGAACTA TGATCTCAAT GACCTTCCTG GATCTCCATC AAAACCTCAG
    GTTACAGATG TCAGTAAGAA CAGTGTTACC CTATCATGGC AACCAGGATC ACCAGGGATT
    CTGCCTGTGA CATCCTACAT CATTGAGGCT TTCAGTCAAT CTGTGAGCAA TAGTTGGCAA
    ACTGTGGCCA ATCACGTGAA GACAACGCTT TACACTGTTA AAGGACTGCG CCCAAACACC
    ATTTATCTCT TTATGGTGAG AGCAATCAAT ACTGAAGGAC TGAGTGACCC AAGTCCAATA
    TCTGATCCAG TGCGTACACA AGATATAAGT CCACCTGTTC AAGGAGTAGA TCACCGGCAA
    GTACAGAAAG AACTTGGGGA TGTGATTGTA CGGCTCCATA ATCCTGTTGT TCTGACACCA
    ACAACAATCC AGGTCACCTG GATGGTCGAC CGTCAATCCC AGTTTATTCA GGGATACAGG
    GTAATGTACC GTCAGACTTC TGGTCTGCAG ACTCCATCTG CGTGGCAGAG CTTAGAGGTC
    AAAGTGCCTG CAGAGCGTAG CGCAGTGCTA ACCAACCTGA AAAAAGGAAT TACTTATGAG
    ATTAAGGTTC GACCTTATTT TAATGAATTT CAAGGAATGG ATAGCGAGTC AAAGTCAGCA
    CGAACAACTG AAGAAGCCCC AAGTGCTCCT CCTCAGTCTG TTACTGTTAT GACAGTGGGA
    AATAACAACA GCACCAGCAT CAGCATCTCA TGGGACCCCC CTCCATCTGA CCACCAGAAT
    GGAATTATCC AGGACTACAG GATCTGGTGC CTGGGTAATG AGACACGTTT CCACATCAAC
    AAGACAGTGG ATGCTGCTAT TCGTTCCGTG GTTATCGGTG GGCTATTCCC TGGAATTCAG
    TACCGCGTTG AAGTCGCAGC CAGTACAAGC GCAGGCACTG GGGTAAAAAG TGCTCCCCAG
    CACATACAGA TAGGAAATCG GAATGAAGTT GTGATCACAG AGAATAACAA CAGCATAACA
    GAGCAAATAA CTGATGTTGT GAAACAGCCG GCATTCATCG CAGGAATTGG TGGTGCCTGC
    TGGGTAATAC TCATGGGATT TAGCATTTGG CTCTACTGGC GAAGAAAGAA GAGAAAGGGT
    CTCAGCAACT ACGCTGTTCA GTCTTTCACC TTTACCCCTG CAGATTCCCC AGATACAACC
    ATGCACCACA AGGTTGCACT TGGCACAAAG AAGGGGAGCT GGCACAAAAC AGTTACATTC
    CAGAGAGGAG ATGGAGGACT AATGAGTAAT GGAAGTCGTC CTGGACTTTT AAATGCGAAT
    GATCCTAGTT ACCCTTGGTT GGCAGACTCC TGGCCAGCTT CCAGCATGCC GGTGAATAAC
    AGCAACAATG GACCAAACGA TCTCGTTAAT TTTAGTTGTG GAGATGTGCT GCCAACAGTT
    CCAGGTCAAG GAGAAAAAAC AGCGACTATG CTTTCTGATG GAGCCATTTA TAGCAGCATT
    GATTTTACTA CAAAATCTAG CTATAATTGC CCAGGACAAA TTGCACAAAC CACGCCATAT
    GCTACTACGC AGATACTACA TTCCAACAGC ATTCATGAGC TGGCAGTTGA CTTGCCTGAT
    CCACAGTGGA AATCTTCCAT TCAGCAGAAA TCTGATCTCA CTGGATTAGG CTATTGCCTT
    ACTGACCAAG GCAAAGGGAG TAATGCCTTA CTTTACATTC CTGACTATCG AATGGCTGAG
    GGATTGTCAA ATAGAATACC CCACAACCAG TCACAGGATT TCAGCACCAC CAGCTCTCAC
    AACAGCTCAG AGAGAAGTGG CAGCCTTTCA GGTGGAAAAG ACGGAAAAAG GAAGAAAAAT
    AAAAACAGCT CTAAAATCTC TAAAAACAAT GGATCCAGTT GGGCCAATGT TCCACTTCCG
    CCCCCTCCCA TCCAACCACT GCCAGGCACT GAAATAGAAC ATTATGGCCT TGAACACCAG
    GAAACAGGCT ATGACACCAA TGGATGGTGC CCACCTTTGC CAGTCCAGAC CTACTTACAT
    CAAGGATCAG ATGATGAACT AGAAGAAGAT GAAGAGAGAG TCCCTACACC CCCTGTACGT
    GGGGTGGCAT CATCACCTGC CATCTCATTT GGACAACAGT CAACAGCAAC CCTCACACCA
    TCACCACGTG AAGAAATGCA ACCCATGCTT CAGGCCCACC TGGATGAATT AACAAGAGCT
    TACCAGTTTG ACATTGCAAA GCAATCTTGG CATTTACACA GCAATAACCA GCCCCCATTG
    GCCCCAGTTC CACCACTTGG ATATGTGTCT GGGACATTTA TTTCTGACTT AGAAAAAGAC
    ATTCCAGATC AAGAAGAAGA TGAAATTGAA GACGAATCTA TAGAAATAAC AAGGCCCCTC
    CGAGGTTTGG AACACACTCC CGGTTCTAGT ATGGATAATC TCGACAGTTC TGTGACAGGC
    AAAATGAACT CCTTTTCTCA GAGACCCCGG CCTGCCAGTC CCTTTTCTAC TGACAGTAAC
    ACAAGTTCAG CTGTGAATCA TAGTCAGAAG TCACGGCCCA CTAAGAAACA CAAGGGAGGC
    AGAATAGGTC AGCAACCCAT GCTTCGCAGA GAAGGGATTG CAGATGATTT GCCTCCCCCA
    CCTGATCCTC CACCTTGTCA AAGACAGCAA ATCATGCCCA GTCAACGTGG TGGAGGTCCT
    ACAGAAAGAA AAGCCAGTTC TCTTGAGAGG CACACATCAA ATATGAATGA TTTAAAAACC
    TCATTAGACC GGCATGGCAG AACATCCTTA GAACGGCAGC GACAAACACA GGAATGGCTT
    GGCTCAACAG AAAGGCAGAA CAAGCAGGCA TTTGGATCTG AAGAGATACT AATGCCATAC
    AGTAAACCTA GCTTCCCATC TCCAGGAGGG CACAGCTCCT CAGGAACAGT CTCTTCAAAA
    GAATCAACTG GACCCAGAAA AGGGGAGGTT TTGAGAGGTC ACCAACGTAA TGCCATTGAC
    TTTCTTGATG TAGGATATTT GGGGCCACAC AGTCAAGGAC AGTTTACAGG TGAATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa51978
    Clone ID Related Accession (Same CDS sequence) XM_031896601.1
    Accession Version XM_031896601.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4671bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product roundabout homolog 2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAACATCT GGATAATGCT ATCTTCACTA AGGCAGAGGG CAGCTTCTCC TGAATTCCTG 
    CTGATCCTTA TGCTGTTCGT CAGTGGGGTG ACCCAAAGCA AAATTAGTAG GACCAAGGGG
    AAGCACTGGA AAGGGGGATC CCGTCTCCGT CAAGAAGATT TCCCCCCACG GATAGTTGAG
    CATCCATCGG ATGTCATTGT GTCAAAGGGA GAGCCAACCA CCTTGAACTG CAAAGCGGAA
    GGGCGGCCTA CACCCACCAT AGAGTGGTAC AAAGACTTAG AAAGGGTTGA GACAGACAAG
    GATGACCCCC GCTCACATCG AATGCTACTG CCAAGTGGAT CTTTATTTTT TCTGCGCATT
    ATCCATGGGC GCAAGTTTAA GCCAGACGAA GGAAGTTACG TCTGTGTGGC AAGGAACTAT
    CTCGGGGAGG CTATGAGTCG AAATGCATCT CTGGAAGTGG CATTGCTCCG TGATGACTTC
    CGGCTCAACC CTACAGATGT TGTTGTGGCA GCTGGGGAAC CTGCAATTTT GGAGTGTCAA
    CCCCCCAGAG GACATCCGGA ACCAACTATA TACTGGAAAA AGGACAAAGT TAGAATTGAT
    GATAAAGATG AAAGAATTAG TATTCGAGGT GGAAAGCTGA TGCTCTCCAA TACAAGGAAA
    AGTGATGCAG GCATGTACAC TTGTGTTGGG ACCAACATGG TTGGAGAAAG AGACAGTGAC
    CCAGCAGAGC TAACAGTCTT TGAACGTCCC ACATTCCTCA GAAGGCCAAT TAATCAAGTT
    GTTTTAGAGG ATGAAGCTGT AGAATTTCGA TGTCAGGTCC AGGGGGACCC ACAGCCAACT
    GTCCGGTGGA AAAAGGATGA TGTAGATTTG CCAAGAGGAA GATACGACAT CAAGGATGAC
    TATACTTTGA GAATCAAGAA GGCTTCCAGC AGCGACGAAG GGACCTTTGC TTGTATTTCT
    GAAAACCGGG TTGGTAAAAT AGAAGCTTCT GCCACACTTA CAGTCAGAGT TCGCCCTGTT
    GCTCCTCCCC AGTTTGTGAT CAGACCAAGG GATCAGATTG TAGCACAAGG CCGGACAGTA
    ACATTTGGAT GTGAAGCTAA AGGAAATCCT CAACCAGCTG TTTTTTGGCA AAAAGAAGGA
    AGCCAGAATT TACTTTTTCC AAATCAGCCT CTTCAGCCCA ACAGCAGGTA CTCAGTGTCA
    CCAACTGGAG ATTTAACCAT CACCAGCATT CAGCGCTCAG ACGCAGGCTA CTACATTTGT
    CAGGCACTGA CTGTTGCCGG AAGCATTCTG GCCAAGGCCC AGCTGGAAGT GACTGATGTT
    TTGACAGATA GACCACCGCC AATAATTCTA CAAGGTCCAG CTAATCAAAC TCTTGCCGTG
    GATGGAACAG CAGTGCTGAA ATGTAAAACT ACTGGGGACC CAATGCCAGT CATAAGTTGG
    CTAAAAGAGG GATTTAAGGG ATTCATAGGG AGAGATGCAA GGTTATCCAT TCTAGACCAA
    GGGACATTAC AAATTAAAGG TTTAAAGGTT ACTGATTCTG GCACCTATAC TTGTGTGGCT
    ACTAGCTCTA GTGGTGAGAC AACATGGAGC GCTGTTCTGG AAGTGACAGA ATCTGGTGGT
    GCCATAATTA GCAAGAACTA TGATCTCAAT GACCTTCCTG GATCTCCATC AAAACCTCAG
    GTTACAGATG TCAGTAAGAA CAGTGTTACC CTATCATGGC AACCAGGATC ACCAGGGATT
    CTGCCTGTGA CATCCTACAT CATTGAGGCT TTCAGTCAAT CTGTGAGCAA TAGTTGGCAA
    ACTGTGGCCA ATCACGTGAA GACAACGCTT TACACTGTTA AAGGACTGCG CCCAAACACC
    ATTTATCTCT TTATGGTGAG AGCAATCAAT ACTGAAGGAC TGAGTGACCC AAGTCCAATA
    TCTGATCCAG TGCGTACACA AGATATAAGT CCACCTGTTC AAGGAGTAGA TCACCGGCAA
    GTACAGAAAG AACTTGGGGA TGTGATTGTA CGGCTCCATA ATCCTGTTGT TCTGACACCA
    ACAACAATCC AGGTCACCTG GATGGTCGAC CGTCAATCCC AGTTTATTCA GGGATACAGG
    GTAATGTACC GTCAGACTTC TGGTCTGCAG ACTCCATCTG CGTGGCAGAG CTTAGAGGTC
    AAAGTGCCTG CAGAGCGTAG CGCAGTGCTA ACCAACCTGA AAAAAGGAAT TACTTATGAG
    ATTAAGGTTC GACCTTATTT TAATGAATTT CAAGGAATGG ATAGCGAGTC AAAGTCAGCA
    CGAACAACTG AAGAAGCCCC AAGTGCTCCT CCTCAGTCTG TTACTGTTAT GACAGTGGGA
    AATAACAACA GCACCAGCAT CAGCATCTCA TGGGACCCCC CTCCATCTGA CCACCAGAAT
    GGAATTATCC AGGACTACAG GATCTGGTGC CTGGGTAATG AGACACGTTT CCACATCAAC
    AAGACAGTGG ATGCTGCTAT TCGTTCCGTG GTTATCGGTG GGCTATTCCC TGGAATTCAG
    TACCGCGTTG AAGTCGCAGC CAGTACAAGC GCAGGCACTG GGGTAAAAAG TGCTCCCCAG
    CACATACAGA TAGGAAATCG GAATGAAGTT GTGATCACAG AGAATAACAA CAGCATAACA
    GAGCAAATAA CTGATGTTGT GAAACAGCCG GCATTCATCG CAGGAATTGG TGGTGCCTGC
    TGGGTAATAC TCATGGGATT TAGCATTTGG CTCTACTGGC GAAGAAAGAA GAGAAAGGGT
    CTCAGCAACT ACGCTGTTCA GTCTTTCACC TTTACCCCTG CAGATTCCCC AGATACAACC
    ATGCACCACA AGGTTGCACT TGGCACAAAG AAGGGGAGCT GGCACAAAAC AGTTACATTC
    CAGAGAGGAG ATGGAGGACT AATGAGTAAT GGAAGTCGTC CTGGACTTTT AAATGCGAAT
    GATCCTAGTT ACCCTTGGTT GGCAGACTCC TGGCCAGCTT CCAGCATGCC GGTGAATAAC
    AGCAACAATG GACCAAACGA TCTCGTTAAT TTTAGTTGTG GAGATGTGCT GCCAACAGTT
    CCAGGTCAAG GAGAAAAAAC AGCGACTATG CTTTCTGATG GAGCCATTTA TAGCAGCATT
    GATTTTACTA CAAAATCTAG CTATAATTGC CCAGGACAAA TTGCACAAAC CACGCCATAT
    GCTACTACGC AGATACTACA TTCCAACAGC ATTCATGAGC TGGCAGTTGA CTTGCCTGAT
    CCACAGTGGA AATCTTCCAT TCAGCAGAAA TCTGATCTCA CTGGATTAGG CTATTGCCTT
    ACTGACCAAG GCAAAGGGAG TAATGCCTTA CTTTACATTC CTGACTATCG AATGGCTGAG
    GGATTGTCAA ATAGAATACC CCACAACCAG TCACAGGATT TCAGCACCAC CAGCTCTCAC
    AACAGCTCAG AGAGAAGTGG CAGCCTTTCA GGTGGAAAAG ACGGAAAAAG GAAGAAAAAT
    AAAAACAGCT CTAAAATCTC TAAAAACAAT GGATCCAGTT GGGCCAATGT TCCACTTCCG
    CCCCCTCCCA TCCAACCACT GCCAGGCACT GAAATAGAAC ATTATGGCCT TGAACACCAG
    GAAACAGGCT ATGACACCAA TGGATGGTGC CCACCTTTGC CAGTCCAGAC CTACTTACAT
    CAAGGATCAG ATGATGAACT AGAAGAAGAT GAAGAGAGAG TCCCTACACC CCCTGTACGT
    GGGGTGGCAT CATCACCTGC CATCTCATTT GGACAACAGT CAACAGCAAC CCTCACACCA
    TCACCACGTG AAGAAATGCA ACCCATGCTT CAGGCCCACC TGGATGAATT AACAAGAGCT
    TACCAGTTTG ACATTGCAAA GCAATCTTGG CATTTACACA GCAATAACCA GCCCCCATTG
    GCCCCAGTTC CACCACTTGG ATATGTGTCT GGGACATTTA TTTCTGACTT AGAAAAAGAC
    ATTCCAGATC AAGAAGAAGA TGAAATTGAA GACGAATCTA TAGAAATAAC AAGGCCCCTC
    CGAGGTTTGG AACACACTCC CGGTTCTAGT ATGGATAATC TCGACAGTTC TGTGACAGGT
    TCAATGGTAA ATGGATGGGG TTCTGCATCT GATGAGGATC GGAACTATTC TAGTCATAGA
    TCTAGTGTAG GTAGCGCAAC AGATGACTCG ATAATTGCAA GTGGCAACTT TGCACAATCA
    CTGGTTGCAG CAGCAGATAA AGCTGGTTTC TCACTAAATG GAAACAGCCT ATCTATAGCA
    GGCAAAATGA ACTCCTTTTC TCAGAGACCC CGGCCTGCCA GTCCCTTTTC TACTGACAGT
    AACACAAGTT CAGCTGTGAA TCATAGTCAG AAGTCACGGC CCACTAAGAA ACACAAGGGA
    GGCAGAATAG GTCAGCAACC CATGCTTCGC AGAGAAGGGA TTGCAGATGA TTTGCCTCCC
    CCACCTGATC CTCCACCTTG TCAAAGACAG CAAATCATGC CCAGTCAACG TGGTGGAGGT
    CCTACAGAAA GAAAAGCCAG TTCTCTTGAG AGGCACACAT CAAATATGAA TGATTTAAAA
    ACCTCATTAG ACCGGCATGG CAGAACATCC TTAGAACGGC AGCGACAAAC ACAGGAATGG
    CTTGGCTCAA CAGAAAGGCA GAACAAGCAG GCATTTGGAT CTGAGTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752461.1
    CDS547..5217
    Translation

    Target ORF information:

    RefSeq Version XM_031896601.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896601.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAACATCT GGATAATGCT ATCTTCACTA AGGCAGAGGG CAGCTTCTCC TGAATTCCTG 
    CTGATCCTTA TGCTGTTCGT CAGTGGGGTG ACCCAAAGCA AAATTAGTAG GACCAAGGGG
    AAGCACTGGA AAGGGGGATC CCGTCTCCGT CAAGAAGATT TCCCCCCACG GATAGTTGAG
    CATCCATCGG ATGTCATTGT GTCAAAGGGA GAGCCAACCA CCTTGAACTG CAAAGCGGAA
    GGGCGGCCTA CACCCACCAT AGAGTGGTAC AAAGACTTAG AAAGGGTTGA GACAGACAAG
    GATGACCCCC GCTCACATCG AATGCTACTG CCAAGTGGAT CTTTATTTTT TCTGCGCATT
    ATCCATGGGC GCAAGTTTAA GCCAGACGAA GGAAGTTACG TCTGTGTGGC AAGGAACTAT
    CTCGGGGAGG CTATGAGTCG AAATGCATCT CTGGAAGTGG CATTGCTCCG TGATGACTTC
    CGGCTCAACC CTACAGATGT TGTTGTGGCA GCTGGGGAAC CTGCAATTTT GGAGTGTCAA
    CCCCCCAGAG GACATCCGGA ACCAACTATA TACTGGAAAA AGGACAAAGT TAGAATTGAT
    GATAAAGATG AAAGAATTAG TATTCGAGGT GGAAAGCTGA TGCTCTCCAA TACAAGGAAA
    AGTGATGCAG GCATGTACAC TTGTGTTGGG ACCAACATGG TTGGAGAAAG AGACAGTGAC
    CCAGCAGAGC TAACAGTCTT TGAACGTCCC ACATTCCTCA GAAGGCCAAT TAATCAAGTT
    GTTTTAGAGG ATGAAGCTGT AGAATTTCGA TGTCAGGTCC AGGGGGACCC ACAGCCAACT
    GTCCGGTGGA AAAAGGATGA TGTAGATTTG CCAAGAGGAA GATACGACAT CAAGGATGAC
    TATACTTTGA GAATCAAGAA GGCTTCCAGC AGCGACGAAG GGACCTTTGC TTGTATTTCT
    GAAAACCGGG TTGGTAAAAT AGAAGCTTCT GCCACACTTA CAGTCAGAGT TCGCCCTGTT
    GCTCCTCCCC AGTTTGTGAT CAGACCAAGG GATCAGATTG TAGCACAAGG CCGGACAGTA
    ACATTTGGAT GTGAAGCTAA AGGAAATCCT CAACCAGCTG TTTTTTGGCA AAAAGAAGGA
    AGCCAGAATT TACTTTTTCC AAATCAGCCT CTTCAGCCCA ACAGCAGGTA CTCAGTGTCA
    CCAACTGGAG ATTTAACCAT CACCAGCATT CAGCGCTCAG ACGCAGGCTA CTACATTTGT
    CAGGCACTGA CTGTTGCCGG AAGCATTCTG GCCAAGGCCC AGCTGGAAGT GACTGATGTT
    TTGACAGATA GACCACCGCC AATAATTCTA CAAGGTCCAG CTAATCAAAC TCTTGCCGTG
    GATGGAACAG CAGTGCTGAA ATGTAAAACT ACTGGGGACC CAATGCCAGT CATAAGTTGG
    CTAAAAGAGG GATTTAAGGG ATTCATAGGG AGAGATGCAA GGTTATCCAT TCTAGACCAA
    GGGACATTAC AAATTAAAGG TTTAAAGGTT ACTGATTCTG GCACCTATAC TTGTGTGGCT
    ACTAGCTCTA GTGGTGAGAC AACATGGAGC GCTGTTCTGG AAGTGACAGA ATCTGGTGGT
    GCCATAATTA GCAAGAACTA TGATCTCAAT GACCTTCCTG GATCTCCATC AAAACCTCAG
    GTTACAGATG TCAGTAAGAA CAGTGTTACC CTATCATGGC AACCAGGATC ACCAGGGATT
    CTGCCTGTGA CATCCTACAT CATTGAGGCT TTCAGTCAAT CTGTGAGCAA TAGTTGGCAA
    ACTGTGGCCA ATCACGTGAA GACAACGCTT TACACTGTTA AAGGACTGCG CCCAAACACC
    ATTTATCTCT TTATGGTGAG AGCAATCAAT ACTGAAGGAC TGAGTGACCC AAGTCCAATA
    TCTGATCCAG TGCGTACACA AGATATAAGT CCACCTGTTC AAGGAGTAGA TCACCGGCAA
    GTACAGAAAG AACTTGGGGA TGTGATTGTA CGGCTCCATA ATCCTGTTGT TCTGACACCA
    ACAACAATCC AGGTCACCTG GATGGTCGAC CGTCAATCCC AGTTTATTCA GGGATACAGG
    GTAATGTACC GTCAGACTTC TGGTCTGCAG ACTCCATCTG CGTGGCAGAG CTTAGAGGTC
    AAAGTGCCTG CAGAGCGTAG CGCAGTGCTA ACCAACCTGA AAAAAGGAAT TACTTATGAG
    ATTAAGGTTC GACCTTATTT TAATGAATTT CAAGGAATGG ATAGCGAGTC AAAGTCAGCA
    CGAACAACTG AAGAAGCCCC AAGTGCTCCT CCTCAGTCTG TTACTGTTAT GACAGTGGGA
    AATAACAACA GCACCAGCAT CAGCATCTCA TGGGACCCCC CTCCATCTGA CCACCAGAAT
    GGAATTATCC AGGACTACAG GATCTGGTGC CTGGGTAATG AGACACGTTT CCACATCAAC
    AAGACAGTGG ATGCTGCTAT TCGTTCCGTG GTTATCGGTG GGCTATTCCC TGGAATTCAG
    TACCGCGTTG AAGTCGCAGC CAGTACAAGC GCAGGCACTG GGGTAAAAAG TGCTCCCCAG
    CACATACAGA TAGGAAATCG GAATGAAGTT GTGATCACAG AGAATAACAA CAGCATAACA
    GAGCAAATAA CTGATGTTGT GAAACAGCCG GCATTCATCG CAGGAATTGG TGGTGCCTGC
    TGGGTAATAC TCATGGGATT TAGCATTTGG CTCTACTGGC GAAGAAAGAA GAGAAAGGGT
    CTCAGCAACT ACGCTGTTCA GTCTTTCACC TTTACCCCTG CAGATTCCCC AGATACAACC
    ATGCACCACA AGGTTGCACT TGGCACAAAG AAGGGGAGCT GGCACAAAAC AGTTACATTC
    CAGAGAGGAG ATGGAGGACT AATGAGTAAT GGAAGTCGTC CTGGACTTTT AAATGCGAAT
    GATCCTAGTT ACCCTTGGTT GGCAGACTCC TGGCCAGCTT CCAGCATGCC GGTGAATAAC
    AGCAACAATG GACCAAACGA TCTCGTTAAT TTTAGTTGTG GAGATGTGCT GCCAACAGTT
    CCAGGTCAAG GAGAAAAAAC AGCGACTATG CTTTCTGATG GAGCCATTTA TAGCAGCATT
    GATTTTACTA CAAAATCTAG CTATAATTGC CCAGGACAAA TTGCACAAAC CACGCCATAT
    GCTACTACGC AGATACTACA TTCCAACAGC ATTCATGAGC TGGCAGTTGA CTTGCCTGAT
    CCACAGTGGA AATCTTCCAT TCAGCAGAAA TCTGATCTCA CTGGATTAGG CTATTGCCTT
    ACTGACCAAG GCAAAGGGAG TAATGCCTTA CTTTACATTC CTGACTATCG AATGGCTGAG
    GGATTGTCAA ATAGAATACC CCACAACCAG TCACAGGATT TCAGCACCAC CAGCTCTCAC
    AACAGCTCAG AGAGAAGTGG CAGCCTTTCA GGTGGAAAAG ACGGAAAAAG GAAGAAAAAT
    AAAAACAGCT CTAAAATCTC TAAAAACAAT GGATCCAGTT GGGCCAATGT TCCACTTCCG
    CCCCCTCCCA TCCAACCACT GCCAGGCACT GAAATAGAAC ATTATGGCCT TGAACACCAG
    GAAACAGGCT ATGACACCAA TGGATGGTGC CCACCTTTGC CAGTCCAGAC CTACTTACAT
    CAAGGATCAG ATGATGAACT AGAAGAAGAT GAAGAGAGAG TCCCTACACC CCCTGTACGT
    GGGGTGGCAT CATCACCTGC CATCTCATTT GGACAACAGT CAACAGCAAC CCTCACACCA
    TCACCACGTG AAGAAATGCA ACCCATGCTT CAGGCCCACC TGGATGAATT AACAAGAGCT
    TACCAGTTTG ACATTGCAAA GCAATCTTGG CATTTACACA GCAATAACCA GCCCCCATTG
    GCCCCAGTTC CACCACTTGG ATATGTGTCT GGGACATTTA TTTCTGACTT AGAAAAAGAC
    ATTCCAGATC AAGAAGAAGA TGAAATTGAA GACGAATCTA TAGAAATAAC AAGGCCCCTC
    CGAGGTTTGG AACACACTCC CGGTTCTAGT ATGGATAATC TCGACAGTTC TGTGACAGGT
    TCAATGGTAA ATGGATGGGG TTCTGCATCT GATGAGGATC GGAACTATTC TAGTCATAGA
    TCTAGTGTAG GTAGCGCAAC AGATGACTCG ATAATTGCAA GTGGCAACTT TGCACAATCA
    CTGGTTGCAG CAGCAGATAA AGCTGGTTTC TCACTAAATG GAAACAGCCT ATCTATAGCA
    GGCAAAATGA ACTCCTTTTC TCAGAGACCC CGGCCTGCCA GTCCCTTTTC TACTGACAGT
    AACACAAGTT CAGCTGTGAA TCATAGTCAG AAGTCACGGC CCACTAAGAA ACACAAGGGA
    GGCAGAATAG GTCAGCAACC CATGCTTCGC AGAGAAGGGA TTGCAGATGA TTTGCCTCCC
    CCACCTGATC CTCCACCTTG TCAAAGACAG CAAATCATGC CCAGTCAACG TGGTGGAGGT
    CCTACAGAAA GAAAAGCCAG TTCTCTTGAG AGGCACACAT CAAATATGAA TGATTTAAAA
    ACCTCATTAG ACCGGCATGG CAGAACATCC TTAGAACGGC AGCGACAAAC ACAGGAATGG
    CTTGGCTCAA CAGAAAGGCA GAACAAGCAG GCATTTGGAT CTGAGTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa51979
    Clone ID Related Accession (Same CDS sequence) XM_031896602.1
    Accession Version XM_031896602.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4641bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product roundabout homolog 2 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAACATCT GGATAATGCT ATCTTCACTA AGGCAGAGGG CAGCTTCTCC TGAATTCCTG 
    CTGATCCTTA TGCTGTTCGT CAGTGGGGTG ACCCAAAGCA AAATTAGTAG GACCAAGGGG
    AAGCACTGGA AAGGGGGATC CCGTCTCCGT CAAGAAGATT TCCCCCCACG GATAGTTGAG
    CATCCATCGG ATGTCATTGT GTCAAAGGGA GAGCCAACCA CCTTGAACTG CAAAGCGGAA
    GGGCGGCCTA CACCCACCAT AGAGTGGTAC AAAGACTTAG AAAGGGTTGA GACAGACAAG
    GATGACCCCC GCTCACATCG AATGCTACTG CCAAGTGGAT CTTTATTTTT TCTGCGCATT
    ATCCATGGGC GCAAGTTTAA GCCAGACGAA GGAAGTTACG TCTGTGTGGC AAGGAACTAT
    CTCGGGGAGG CTATGAGTCG AAATGCATCT CTGGAAGTGG CATTGCTCCG TGATGACTTC
    CGGCTCAACC CTACAGATGT TGTTGTGGCA GCTGGGGAAC CTGCAATTTT GGAGTGTCAA
    CCCCCCAGAG GACATCCGGA ACCAACTATA TACTGGAAAA AGGACAAAGT TAGAATTGAT
    GATAAAGATG AAAGAATTAG TATTCGAGGT GGAAAGCTGA TGCTCTCCAA TACAAGGAAA
    AGTGATGCAG GCATGTACAC TTGTGTTGGG ACCAACATGG TTGGAGAAAG AGACAGTGAC
    CCAGCAGAGC TAACAGTCTT TGAACGTCCC ACATTCCTCA GAAGGCCAAT TAATCAAGTT
    GTTTTAGAGG ATGAAGCTGT AGAATTTCGA TGTCAGGTCC AGGGGGACCC ACAGCCAACT
    GTCCGGTGGA AAAAGGATGA TGTAGATTTG CCAAGAGGAA GATACGACAT CAAGGATGAC
    TATACTTTGA GAATCAAGAA GGCTTCCAGC AGCGACGAAG GGACCTTTGC TTGTATTTCT
    GAAAACCGGG TTGGTAAAAT AGAAGCTTCT GCCACACTTA CAGTCAGAGT TCGCCCTGTT
    GCTCCTCCCC AGTTTGTGAT CAGACCAAGG GATCAGATTG TAGCACAAGG CCGGACAGTA
    ACATTTGGAT GTGAAGCTAA AGGAAATCCT CAACCAGCTG TTTTTTGGCA AAAAGAAGGA
    AGCCAGAATT TACTTTTTCC AAATCAGCCT CTTCAGCCCA ACAGCAGGTA CTCAGTGTCA
    CCAACTGGAG ATTTAACCAT CACCAGCATT CAGCGCTCAG ACGCAGGCTA CTACATTTGT
    CAGGCACTGA CTGTTGCCGG AAGCATTCTG GCCAAGGCCC AGCTGGAAGT GACTGATGTT
    TTGACAGATA GACCACCGCC AATAATTCTA CAAGGTCCAG CTAATCAAAC TCTTGCCGTG
    GATGGAACAG CAGTGCTGAA ATGTAAAACT ACTGGGGACC CAATGCCAGT CATAAGTTGG
    CTAAAAGAGG GATTTAAGGG ATTCATAGGG AGAGATGCAA GGTTATCCAT TCTAGACCAA
    GGGACATTAC AAATTAAAGG TTTAAAGGTT ACTGATTCTG GCACCTATAC TTGTGTGGCT
    ACTAGCTCTA GTGGTGAGAC AACATGGAGC GCTGTTCTGG AAGTGACAGA ATCTGGTGGT
    GCCATAATTA GCAAGAACTA TGATCTCAAT GACCTTCCTG GATCTCCATC AAAACCTCAG
    GTTACAGATG TCAGTAAGAA CAGTGTTACC CTATCATGGC AACCAGGATC ACCAGGGATT
    CTGCCTGTGA CATCCTACAT CATTGAGGCT TTCAGTCAAT CTGTGAGCAA TAGTTGGCAA
    ACTGTGGCCA ATCACGTGAA GACAACGCTT TACACTGTTA AAGGACTGCG CCCAAACACC
    ATTTATCTCT TTATGGTGAG AGCAATCAAT ACTGAAGGAC TGAGTGACCC AAGTCCAATA
    TCTGATCCAG TGCGTACACA AGATATAAGT CCACCTGTTC AAGGAGTAGA TCACCGGCAA
    GTACAGAAAG AACTTGGGGA TGTGATTGTA CGGCTCCATA ATCCTGTTGT TCTGACACCA
    ACAACAATCC AGGTCACCTG GATGGTCGAC CGTCAATCCC AGTTTATTCA GGGATACAGG
    GTAATGTACC GTCAGACTTC TGGTCTGCAG ACTCCATCTG CGTGGCAGAG CTTAGAGGTC
    AAAGTGCCTG CAGAGCGTAG CGCAGTGCTA ACCAACCTGA AAAAAGGAAT TACTTATGAG
    ATTAAGGTTC GACCTTATTT TAATGAATTT CAAGGAATGG ATAGCGAGTC AAAGTCAGCA
    CGAACAACTG AAGAAGCCCC AAGTGCTCCT CCTCAGTCTG TTACTGTTAT GACAGTGGGA
    AATAACAACA GCACCAGCAT CAGCATCTCA TGGGACCCCC CTCCATCTGA CCACCAGAAT
    GGAATTATCC AGGACTACAG GATCTGGTGC CTGGGTAATG AGACACGTTT CCACATCAAC
    AAGACAGTGG ATGCTGCTAT TCGTTCCGTG GTTATCGGTG GGCTATTCCC TGGAATTCAG
    TACCGCGTTG AAGTCGCAGC CAGTACAAGC GCAGGCACTG GGGTAAAAAG TGCTCCCCAG
    CACATACAGA TAGGAAATCG GAATGAAGTT GTGATCACAG AGAATAACAA CAGCATAACA
    GAGCAAATAA CTGATGTTGT GAAACAGCCG GCATTCATCG CAGGAATTGG TGGTGCCTGC
    TGGGTAATAC TCATGGGATT TAGCATTTGG CTCTACTGGC GAAGAAAGAA GAGAAAGGGT
    CTCAGCAACT ACGCTGTTAC ATTCCAGAGA GGAGATGGAG GACTAATGAG TAATGGAAGT
    CGTCCTGGAC TTTTAAATGC GAATGATCCT AGTTACCCTT GGTTGGCAGA CTCCTGGCCA
    GCTTCCAGCA TGCCGGTGAA TAACAGCAAC AATGGACCAA ACGATCTCGT TAATTTTAGT
    TGTGGAGATG TGCTGCCAAC AGTTCCAGGT CAAGGAGAAA AAACAGCGAC TATGCTTTCT
    GATGGAGCCA TTTATAGCAG CATTGATTTT ACTACAAAAT CTAGCTATAA TTGCCCAGGA
    CAAATTGCAC AAACCACGCC ATATGCTACT ACGCAGATAC TACATTCCAA CAGCATTCAT
    GAGCTGGCAG TTGACTTGCC TGATCCACAG TGGAAATCTT CCATTCAGCA GAAATCTGAT
    CTCACTGGAT TAGGCTATTG CCTTACTGAC CAAGGCAAAG GGAGTAATGG TGGAAAAGAC
    GGAAAAAGGA AGAAAAATAA AAACAGCTCT AAAATCTCTA AAAACAATGG ATCCAGTTGG
    GCCAATGTTC CACTTCCGCC CCCTCCCATC CAACCACTGC CAGGCACTGA AATAGAACAT
    TATGGCCTTG AACACCAGGA AACAGGCTAT GACACCAATG GATGGTGCCC ACCTTTGCCA
    GTCCAGACCT ACTTACATCA AGGATCAGAT GATGAACTAG AAGAAGATGA AGAGAGAGTC
    CCTACACCCC CTGTACGTGG GGTGGCATCA TCACCTGCCA TCTCATTTGG ACAACAGTCA
    ACAGCAACCC TCACACCATC ACCACGTGAA GAAATGCAAC CCATGCTTCA GGCCCACCTG
    GATGAATTAA CAAGAGCTTA CCAGTTTGAC ATTGCAAAGC AATCTTGGCA TTTACACAGC
    AATAACCAGC CCCCATTGGC CCCAGTTCCA CCACTTGGAT ATGTGTCTGG GACATTTATT
    TCTGACTTAG AAAAAGACAT TCCAGATCAA GAAGAAGATG AAATTGAAGA CGAATCTATA
    GAAATAACAA GGCCCCTCCG AGGTTTGGAA CACACTCCCG GTTCTAGTAT GGATAATCTC
    GACAGTTCTG TGACAGGTTC AATGGTAAAT GGATGGGGTT CTGCATCTGA TGAGGATCGG
    AACTATTCTA GTCATAGATC TAGTGTAGGT AGCGCAACAG ATGACTCGAT AATTGCAAGT
    GGCAACTTTG CACAATCACT GGTTGCAGCA GCAGATAAAG CTGGTTTCTC ACTAAATGGA
    AACAGCCTAT CTATAGCAGG CAAAATGAAC TCCTTTTCTC AGAGACCCCG GCCTGCCAGT
    CCCTTTTCTA CTGACAGTAA CACAAGTTCA GCTGTGAATC ATAGTCAGAA GTCACGGCCC
    ACTAAGAAAC ACAAGGGAGG CAGAATAGGT CAGCAACCCA TGCTTCGCAG AGAAGGGATT
    GCAGATGATT TGCCTCCCCC ACCTGATCCT CCACCTTGTC AAAGACAGCA AATCATGCCC
    AGTCAACGTG GTGGAGGTCC TACAGAAAGA AAAGCCAGTT CTCTTGAGAG GCACACATCA
    AATATGAATG ATTTAAAAAC CTCATTAGAC CGGCATGGCA GAACATCCTT AGAACGGCAG
    CGACAAACAC AGGAATGGCT TGGCTCAACA GAAAGGCAGA ACAAGCAGGC ATTTGGATCT
    GAAGAGATAC TAATGCCATA CAGTAAACCT AGCTTCCCAT CTCCAGGAGG GCACAGCTCC
    TCAGGAACAG TCTCTTCAAA AGAATCAACT GGACCCAGAA AAGGGGAGGT TTTGAGAGGT
    CACCAACGTA ATGCCATTGA CTTTCTTGAT GTAGGATATT TGGGGCCACA CAGTCAAGGA
    CAGTTTACAG GTGAATTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752462.1
    CDS546..5186
    Translation

    Target ORF information:

    RefSeq Version XM_031896602.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896602.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAACATCT GGATAATGCT ATCTTCACTA AGGCAGAGGG CAGCTTCTCC TGAATTCCTG 
    CTGATCCTTA TGCTGTTCGT CAGTGGGGTG ACCCAAAGCA AAATTAGTAG GACCAAGGGG
    AAGCACTGGA AAGGGGGATC CCGTCTCCGT CAAGAAGATT TCCCCCCACG GATAGTTGAG
    CATCCATCGG ATGTCATTGT GTCAAAGGGA GAGCCAACCA CCTTGAACTG CAAAGCGGAA
    GGGCGGCCTA CACCCACCAT AGAGTGGTAC AAAGACTTAG AAAGGGTTGA GACAGACAAG
    GATGACCCCC GCTCACATCG AATGCTACTG CCAAGTGGAT CTTTATTTTT TCTGCGCATT
    ATCCATGGGC GCAAGTTTAA GCCAGACGAA GGAAGTTACG TCTGTGTGGC AAGGAACTAT
    CTCGGGGAGG CTATGAGTCG AAATGCATCT CTGGAAGTGG CATTGCTCCG TGATGACTTC
    CGGCTCAACC CTACAGATGT TGTTGTGGCA GCTGGGGAAC CTGCAATTTT GGAGTGTCAA
    CCCCCCAGAG GACATCCGGA ACCAACTATA TACTGGAAAA AGGACAAAGT TAGAATTGAT
    GATAAAGATG AAAGAATTAG TATTCGAGGT GGAAAGCTGA TGCTCTCCAA TACAAGGAAA
    AGTGATGCAG GCATGTACAC TTGTGTTGGG ACCAACATGG TTGGAGAAAG AGACAGTGAC
    CCAGCAGAGC TAACAGTCTT TGAACGTCCC ACATTCCTCA GAAGGCCAAT TAATCAAGTT
    GTTTTAGAGG ATGAAGCTGT AGAATTTCGA TGTCAGGTCC AGGGGGACCC ACAGCCAACT
    GTCCGGTGGA AAAAGGATGA TGTAGATTTG CCAAGAGGAA GATACGACAT CAAGGATGAC
    TATACTTTGA GAATCAAGAA GGCTTCCAGC AGCGACGAAG GGACCTTTGC TTGTATTTCT
    GAAAACCGGG TTGGTAAAAT AGAAGCTTCT GCCACACTTA CAGTCAGAGT TCGCCCTGTT
    GCTCCTCCCC AGTTTGTGAT CAGACCAAGG GATCAGATTG TAGCACAAGG CCGGACAGTA
    ACATTTGGAT GTGAAGCTAA AGGAAATCCT CAACCAGCTG TTTTTTGGCA AAAAGAAGGA
    AGCCAGAATT TACTTTTTCC AAATCAGCCT CTTCAGCCCA ACAGCAGGTA CTCAGTGTCA
    CCAACTGGAG ATTTAACCAT CACCAGCATT CAGCGCTCAG ACGCAGGCTA CTACATTTGT
    CAGGCACTGA CTGTTGCCGG AAGCATTCTG GCCAAGGCCC AGCTGGAAGT GACTGATGTT
    TTGACAGATA GACCACCGCC AATAATTCTA CAAGGTCCAG CTAATCAAAC TCTTGCCGTG
    GATGGAACAG CAGTGCTGAA ATGTAAAACT ACTGGGGACC CAATGCCAGT CATAAGTTGG
    CTAAAAGAGG GATTTAAGGG ATTCATAGGG AGAGATGCAA GGTTATCCAT TCTAGACCAA
    GGGACATTAC AAATTAAAGG TTTAAAGGTT ACTGATTCTG GCACCTATAC TTGTGTGGCT
    ACTAGCTCTA GTGGTGAGAC AACATGGAGC GCTGTTCTGG AAGTGACAGA ATCTGGTGGT
    GCCATAATTA GCAAGAACTA TGATCTCAAT GACCTTCCTG GATCTCCATC AAAACCTCAG
    GTTACAGATG TCAGTAAGAA CAGTGTTACC CTATCATGGC AACCAGGATC ACCAGGGATT
    CTGCCTGTGA CATCCTACAT CATTGAGGCT TTCAGTCAAT CTGTGAGCAA TAGTTGGCAA
    ACTGTGGCCA ATCACGTGAA GACAACGCTT TACACTGTTA AAGGACTGCG CCCAAACACC
    ATTTATCTCT TTATGGTGAG AGCAATCAAT ACTGAAGGAC TGAGTGACCC AAGTCCAATA
    TCTGATCCAG TGCGTACACA AGATATAAGT CCACCTGTTC AAGGAGTAGA TCACCGGCAA
    GTACAGAAAG AACTTGGGGA TGTGATTGTA CGGCTCCATA ATCCTGTTGT TCTGACACCA
    ACAACAATCC AGGTCACCTG GATGGTCGAC CGTCAATCCC AGTTTATTCA GGGATACAGG
    GTAATGTACC GTCAGACTTC TGGTCTGCAG ACTCCATCTG CGTGGCAGAG CTTAGAGGTC
    AAAGTGCCTG CAGAGCGTAG CGCAGTGCTA ACCAACCTGA AAAAAGGAAT TACTTATGAG
    ATTAAGGTTC GACCTTATTT TAATGAATTT CAAGGAATGG ATAGCGAGTC AAAGTCAGCA
    CGAACAACTG AAGAAGCCCC AAGTGCTCCT CCTCAGTCTG TTACTGTTAT GACAGTGGGA
    AATAACAACA GCACCAGCAT CAGCATCTCA TGGGACCCCC CTCCATCTGA CCACCAGAAT
    GGAATTATCC AGGACTACAG GATCTGGTGC CTGGGTAATG AGACACGTTT CCACATCAAC
    AAGACAGTGG ATGCTGCTAT TCGTTCCGTG GTTATCGGTG GGCTATTCCC TGGAATTCAG
    TACCGCGTTG AAGTCGCAGC CAGTACAAGC GCAGGCACTG GGGTAAAAAG TGCTCCCCAG
    CACATACAGA TAGGAAATCG GAATGAAGTT GTGATCACAG AGAATAACAA CAGCATAACA
    GAGCAAATAA CTGATGTTGT GAAACAGCCG GCATTCATCG CAGGAATTGG TGGTGCCTGC
    TGGGTAATAC TCATGGGATT TAGCATTTGG CTCTACTGGC GAAGAAAGAA GAGAAAGGGT
    CTCAGCAACT ACGCTGTTAC ATTCCAGAGA GGAGATGGAG GACTAATGAG TAATGGAAGT
    CGTCCTGGAC TTTTAAATGC GAATGATCCT AGTTACCCTT GGTTGGCAGA CTCCTGGCCA
    GCTTCCAGCA TGCCGGTGAA TAACAGCAAC AATGGACCAA ACGATCTCGT TAATTTTAGT
    TGTGGAGATG TGCTGCCAAC AGTTCCAGGT CAAGGAGAAA AAACAGCGAC TATGCTTTCT
    GATGGAGCCA TTTATAGCAG CATTGATTTT ACTACAAAAT CTAGCTATAA TTGCCCAGGA
    CAAATTGCAC AAACCACGCC ATATGCTACT ACGCAGATAC TACATTCCAA CAGCATTCAT
    GAGCTGGCAG TTGACTTGCC TGATCCACAG TGGAAATCTT CCATTCAGCA GAAATCTGAT
    CTCACTGGAT TAGGCTATTG CCTTACTGAC CAAGGCAAAG GGAGTAATGG TGGAAAAGAC
    GGAAAAAGGA AGAAAAATAA AAACAGCTCT AAAATCTCTA AAAACAATGG ATCCAGTTGG
    GCCAATGTTC CACTTCCGCC CCCTCCCATC CAACCACTGC CAGGCACTGA AATAGAACAT
    TATGGCCTTG AACACCAGGA AACAGGCTAT GACACCAATG GATGGTGCCC ACCTTTGCCA
    GTCCAGACCT ACTTACATCA AGGATCAGAT GATGAACTAG AAGAAGATGA AGAGAGAGTC
    CCTACACCCC CTGTACGTGG GGTGGCATCA TCACCTGCCA TCTCATTTGG ACAACAGTCA
    ACAGCAACCC TCACACCATC ACCACGTGAA GAAATGCAAC CCATGCTTCA GGCCCACCTG
    GATGAATTAA CAAGAGCTTA CCAGTTTGAC ATTGCAAAGC AATCTTGGCA TTTACACAGC
    AATAACCAGC CCCCATTGGC CCCAGTTCCA CCACTTGGAT ATGTGTCTGG GACATTTATT
    TCTGACTTAG AAAAAGACAT TCCAGATCAA GAAGAAGATG AAATTGAAGA CGAATCTATA
    GAAATAACAA GGCCCCTCCG AGGTTTGGAA CACACTCCCG GTTCTAGTAT GGATAATCTC
    GACAGTTCTG TGACAGGTTC AATGGTAAAT GGATGGGGTT CTGCATCTGA TGAGGATCGG
    AACTATTCTA GTCATAGATC TAGTGTAGGT AGCGCAACAG ATGACTCGAT AATTGCAAGT
    GGCAACTTTG CACAATCACT GGTTGCAGCA GCAGATAAAG CTGGTTTCTC ACTAAATGGA
    AACAGCCTAT CTATAGCAGG CAAAATGAAC TCCTTTTCTC AGAGACCCCG GCCTGCCAGT
    CCCTTTTCTA CTGACAGTAA CACAAGTTCA GCTGTGAATC ATAGTCAGAA GTCACGGCCC
    ACTAAGAAAC ACAAGGGAGG CAGAATAGGT CAGCAACCCA TGCTTCGCAG AGAAGGGATT
    GCAGATGATT TGCCTCCCCC ACCTGATCCT CCACCTTGTC AAAGACAGCA AATCATGCCC
    AGTCAACGTG GTGGAGGTCC TACAGAAAGA AAAGCCAGTT CTCTTGAGAG GCACACATCA
    AATATGAATG ATTTAAAAAC CTCATTAGAC CGGCATGGCA GAACATCCTT AGAACGGCAG
    CGACAAACAC AGGAATGGCT TGGCTCAACA GAAAGGCAGA ACAAGCAGGC ATTTGGATCT
    GAAGAGATAC TAATGCCATA CAGTAAACCT AGCTTCCCAT CTCCAGGAGG GCACAGCTCC
    TCAGGAACAG TCTCTTCAAA AGAATCAACT GGACCCAGAA AAGGGGAGGT TTTGAGAGGT
    CACCAACGTA ATGCCATTGA CTTTCTTGAT GTAGGATATT TGGGGCCACA CAGTCAAGGA
    CAGTTTACAG GTGAATTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa51980
    Clone ID Related Accession (Same CDS sequence) XM_031896603.1
    Accession Version XM_031896603.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4584bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product roundabout homolog 2 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAACATCT GGATAATGCT ATCTTCACTA AGGCAGAGGG CAGCTTCTCC TGAATTCCTG 
    CTGATCCTTA TGCTGTTCGT CAGTGGGGTG ACCCAAAGCA AAATTAGTAG GACCAAGGGG
    AAGCACTGGA AAGGGGGATC CCGTCTCCGT CAAGAAGATT TCCCCCCACG GATAGTTGAG
    CATCCATCGG ATGTCATTGT GTCAAAGGGA GAGCCAACCA CCTTGAACTG CAAAGCGGAA
    GGGCGGCCTA CACCCACCAT AGAGTGGTAC AAAGACTTAG AAAGGGTTGA GACAGACAAG
    GATGACCCCC GCTCACATCG AATGCTACTG CCAAGTGGAT CTTTATTTTT TCTGCGCATT
    ATCCATGGGC GCAAGTTTAA GCCAGACGAA GGAAGTTACG TCTGTGTGGC AAGGAACTAT
    CTCGGGGAGG CTATGAGTCG AAATGCATCT CTGGAAGTGG CATTGCTCCG TGATGACTTC
    CGGCTCAACC CTACAGATGT TGTTGTGGCA GCTGGGGAAC CTGCAATTTT GGAGTGTCAA
    CCCCCCAGAG GACATCCGGA ACCAACTATA TACTGGAAAA AGGACAAAGT TAGAATTGAT
    GATAAAGATG AAAGAATTAG TATTCGAGGT GGAAAGCTGA TGCTCTCCAA TACAAGGAAA
    AGTGATGCAG GCATGTACAC TTGTGTTGGG ACCAACATGG TTGGAGAAAG AGACAGTGAC
    CCAGCAGAGC TAACAGTCTT TGAACGTCCC ACATTCCTCA GAAGGCCAAT TAATCAAGTT
    GTTTTAGAGG ATGAAGCTGT AGAATTTCGA TGTCAGGTCC AGGGGGACCC ACAGCCAACT
    GTCCGGTGGA AAAAGGATGA TGTAGATTTG CCAAGAGGAA GATACGACAT CAAGGATGAC
    TATACTTTGA GAATCAAGAA GGCTTCCAGC AGCGACGAAG GGACCTTTGC TTGTATTTCT
    GAAAACCGGG TTGGTAAAAT AGAAGCTTCT GCCACACTTA CAGTCAGAGT TCGCCCTGTT
    GCTCCTCCCC AGTTTGTGAT CAGACCAAGG GATCAGATTG TAGCACAAGG CCGGACAGTA
    ACATTTGGAT GTGAAGCTAA AGGAAATCCT CAACCAGCTG TTTTTTGGCA AAAAGAAGGA
    AGCCAGAATT TACTTTTTCC AAATCAGCCT CTTCAGCCCA ACAGCAGGTA CTCAGTGTCA
    CCAACTGGAG ATTTAACCAT CACCAGCATT CAGCGCTCAG ACGCAGGCTA CTACATTTGT
    CAGGCACTGA CTGTTGCCGG AAGCATTCTG GCCAAGGCCC AGCTGGAAGT GACTGATGTT
    TTGACAGATA GACCACCGCC AATAATTCTA CAAGGTCCAG CTAATCAAAC TCTTGCCGTG
    GATGGAACAG CAGTGCTGAA ATGTAAAACT ACTGGGGACC CAATGCCAGT CATAAGTTGG
    CTAAAAGAGG GATTTAAGGG ATTCATAGGG AGAGATGCAA GGTTATCCAT TCTAGACCAA
    GGGACATTAC AAATTAAAGG TTTAAAGGTT ACTGATTCTG GCACCTATAC TTGTGTGGCT
    ACTAGCTCTA GTGGTGAGAC AACATGGAGC GCTGTTCTGG AAGTGACAGA ATCTGGTGGT
    GCCATAATTA GCAAGAACTA TGATCTCAAT GACCTTCCTG GATCTCCATC AAAACCTCAG
    GTTACAGATG TCAGTAAGAA CAGTGTTACC CTATCATGGC AACCAGGATC ACCAGGGATT
    CTGCCTGTGA CATCCTACAT CATTGAGGCT TTCAGTCAAT CTGTGAGCAA TAGTTGGCAA
    ACTGTGGCCA ATCACGTGAA GACAACGCTT TACACTGTTA AAGGACTGCG CCCAAACACC
    ATTTATCTCT TTATGGTGAG AGCAATCAAT ACTGAAGGAC TGAGTGACCC AAGTCCAATA
    TCTGATCCAG TGCGTACACA AGATATAAGT CCACCTGTTC AAGGAGTAGA TCACCGGCAA
    GTACAGAAAG AACTTGGGGA TGTGATTGTA CGGCTCCATA ATCCTGTTGT TCTGACACCA
    ACAACAATCC AGGTCACCTG GATGGTCGAC CGTCAATCCC AGTTTATTCA GGGATACAGG
    GTAATGTACC GTCAGACTTC TGGTCTGCAG ACTCCATCTG CGTGGCAGAG CTTAGAGGTC
    AAAGTGCCTG CAGAGCGTAG CGCAGTGCTA ACCAACCTGA AAAAAGGAAT TACTTATGAG
    ATTAAGGTTC GACCTTATTT TAATGAATTT CAAGGAATGG ATAGCGAGTC AAAGTCAGCA
    CGAACAACTG AAGAAGCCCC AAGTGCTCCT CCTCAGTCTG TTACTGTTAT GACAGTGGGA
    AATAACAACA GCACCAGCAT CAGCATCTCA TGGGACCCCC CTCCATCTGA CCACCAGAAT
    GGAATTATCC AGGACTACAG GATCTGGTGC CTGGGTAATG AGACACGTTT CCACATCAAC
    AAGACAGTGG ATGCTGCTAT TCGTTCCGTG GTTATCGGTG GGCTATTCCC TGGAATTCAG
    TACCGCGTTG AAGTCGCAGC CAGTACAAGC GCAGGCACTG GGGTAAAAAG TGCTCCCCAG
    CACATACAGA TAGGAAATCG GAATGAAGTT GTGATCACAG AGAATAACAA CAGCATAACA
    GAGCAAATAA CTGATGTTGT GAAACAGCCG GCATTCATCG CAGGAATTGG TGGTGCCTGC
    TGGGTAATAC TCATGGGATT TAGCATTTGG CTCTACTGGC GAAGAAAGAA GAGAAAGGGT
    CTCAGCAACT ACGCTGTTAC ATTCCAGAGA GGAGATGGAG GACTAATGAG TAATGGAAGT
    CGTCCTGGAC TTTTAAATGC GAATGATCCT AGTTACCCTT GGTTGGCAGA CTCCTGGCCA
    GCTTCCAGCA TGCCGGTGAA TAACAGCAAC AATGGACCAA ACGATCTCGT TAATTTTAGT
    TGTGGAGATG TGCTGCCAAC AGTTCCAGGT CAAGGAGAAA AAACAGCGAC TATGCTTTCT
    GATGGAGCCA TTTATAGCAG CATTGATTTT ACTACAAAAT CTAGCTATAA TTGCCCAGGA
    CAAATTGCAC AAACCACGCC ATATGCTACT ACGCAGATAC TACATTCCAA CAGCATTCAT
    GAGCTGGCAG TTGACTTGCC TGATCCACAG TGGAAATCTT CCATTCAGCA GAAATCTGAT
    CTCACTGGAT TAGGCTATTG CCTTACTGAC CAAGGCAAAG GGAGTAATGC CTTACTTTAC
    ATTCCTGACT ATCGAATGGC TGAGGGATTG TCAAATAGAA TACCCCACAA CCAGTCACAG
    GATTTCAGCA CCACCAGCTC TCACAACAGC TCAGAGAGAA GTGGCAGCCT TTCAGGTGGA
    AAAGACGGAA AAAGGAAGAA AAATAAAAAC AGCTCTAAAA TCTCTAAAAA CAATGGATCC
    AGTTGGGCCA ATGTTCCACT TCCGCCCCCT CCCATCCAAC CACTGCCAGG CACTGAAATA
    GAACATTATG GCCTTGAACA CCAGGAAACA GGCTATGACA CCAATGGATG GTGCCCACCT
    TTGCCAGTCC AGACCTACTT ACATCAAGGA TCAGATGATG AACTAGAAGA AGATGAAGAG
    AGAGTCCCTA CACCCCCTGT ACGTGGGGTG GCATCATCAC CTGCCATCTC ATTTGGACAA
    CAGTCAACAG CAACCCTCAC ACCATCACCA CGTGAAGAAA TGCAACCCAT GCTTCAGGCC
    CACCTGGATG AATTAACAAG AGCTTACCAG TTTGACATTG CAAAGCAATC TTGGCATTTA
    CACAGCAATA ACCAGCCCCC ATTGGCCCCA GTTCCACCAC TTGGATATGT GTCTGGGACA
    TTTATTTCTG ACTTAGAAAA AGACATTCCA GATCAAGAAG AAGATGAAAT TGAAGACGAA
    TCTATAGAAA TAACAAGGCC CCTCCGAGGT TTGGAACACA CTCCCGGTTC TAGTATGGAT
    AATCTCGACA GTTCTGTGAC AGGCAAAATG AACTCCTTTT CTCAGAGACC CCGGCCTGCC
    AGTCCCTTTT CTACTGACAG TAACACAAGT TCAGCTGTGA ATCATAGTCA GAAGTCACGG
    CCCACTAAGA AACACAAGGG AGGCAGAATA GGTCAGCAAC CCATGCTTCG CAGAGAAGGG
    ATTGCAGATG ATTTGCCTCC CCCACCTGAT CCTCCACCTT GTCAAAGACA GCAAATCATG
    CCCAGTCAAC GTGGTGGAGG TCCTACAGAA AGAAAAGCCA GTTCTCTTGA GAGGCACACA
    TCAAATATGA ATGATTTAAA AACCTCATTA GACCGGCATG GCAGAACATC CTTAGAACGG
    CAGCGACAAA CACAGGAATG GCTTGGCTCA ACAGAAAGGC AGAACAAGCA GGCATTTGGA
    TCTGAAGAGA TACTAATGCC ATACAGTAAA CCTAGCTTCC CATCTCCAGG AGGGCACAGC
    TCCTCAGGAA CAGTCTCTTC AAAAGAATCA ACTGGACCCA GAAAAGGGGA GGTTTTGAGA
    GGTCACCAAC GTAATGCCAT TGACTTTCTT GATGTAGGAT ATTTGGGGCC ACACAGTCAA
    GGACAGTTTA CAGGTGAATT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752463.1
    CDS542..5125
    Translation

    Target ORF information:

    RefSeq Version XM_031896603.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896603.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAACATCT GGATAATGCT ATCTTCACTA AGGCAGAGGG CAGCTTCTCC TGAATTCCTG 
    CTGATCCTTA TGCTGTTCGT CAGTGGGGTG ACCCAAAGCA AAATTAGTAG GACCAAGGGG
    AAGCACTGGA AAGGGGGATC CCGTCTCCGT CAAGAAGATT TCCCCCCACG GATAGTTGAG
    CATCCATCGG ATGTCATTGT GTCAAAGGGA GAGCCAACCA CCTTGAACTG CAAAGCGGAA
    GGGCGGCCTA CACCCACCAT AGAGTGGTAC AAAGACTTAG AAAGGGTTGA GACAGACAAG
    GATGACCCCC GCTCACATCG AATGCTACTG CCAAGTGGAT CTTTATTTTT TCTGCGCATT
    ATCCATGGGC GCAAGTTTAA GCCAGACGAA GGAAGTTACG TCTGTGTGGC AAGGAACTAT
    CTCGGGGAGG CTATGAGTCG AAATGCATCT CTGGAAGTGG CATTGCTCCG TGATGACTTC
    CGGCTCAACC CTACAGATGT TGTTGTGGCA GCTGGGGAAC CTGCAATTTT GGAGTGTCAA
    CCCCCCAGAG GACATCCGGA ACCAACTATA TACTGGAAAA AGGACAAAGT TAGAATTGAT
    GATAAAGATG AAAGAATTAG TATTCGAGGT GGAAAGCTGA TGCTCTCCAA TACAAGGAAA
    AGTGATGCAG GCATGTACAC TTGTGTTGGG ACCAACATGG TTGGAGAAAG AGACAGTGAC
    CCAGCAGAGC TAACAGTCTT TGAACGTCCC ACATTCCTCA GAAGGCCAAT TAATCAAGTT
    GTTTTAGAGG ATGAAGCTGT AGAATTTCGA TGTCAGGTCC AGGGGGACCC ACAGCCAACT
    GTCCGGTGGA AAAAGGATGA TGTAGATTTG CCAAGAGGAA GATACGACAT CAAGGATGAC
    TATACTTTGA GAATCAAGAA GGCTTCCAGC AGCGACGAAG GGACCTTTGC TTGTATTTCT
    GAAAACCGGG TTGGTAAAAT AGAAGCTTCT GCCACACTTA CAGTCAGAGT TCGCCCTGTT
    GCTCCTCCCC AGTTTGTGAT CAGACCAAGG GATCAGATTG TAGCACAAGG CCGGACAGTA
    ACATTTGGAT GTGAAGCTAA AGGAAATCCT CAACCAGCTG TTTTTTGGCA AAAAGAAGGA
    AGCCAGAATT TACTTTTTCC AAATCAGCCT CTTCAGCCCA ACAGCAGGTA CTCAGTGTCA
    CCAACTGGAG ATTTAACCAT CACCAGCATT CAGCGCTCAG ACGCAGGCTA CTACATTTGT
    CAGGCACTGA CTGTTGCCGG AAGCATTCTG GCCAAGGCCC AGCTGGAAGT GACTGATGTT
    TTGACAGATA GACCACCGCC AATAATTCTA CAAGGTCCAG CTAATCAAAC TCTTGCCGTG
    GATGGAACAG CAGTGCTGAA ATGTAAAACT ACTGGGGACC CAATGCCAGT CATAAGTTGG
    CTAAAAGAGG GATTTAAGGG ATTCATAGGG AGAGATGCAA GGTTATCCAT TCTAGACCAA
    GGGACATTAC AAATTAAAGG TTTAAAGGTT ACTGATTCTG GCACCTATAC TTGTGTGGCT
    ACTAGCTCTA GTGGTGAGAC AACATGGAGC GCTGTTCTGG AAGTGACAGA ATCTGGTGGT
    GCCATAATTA GCAAGAACTA TGATCTCAAT GACCTTCCTG GATCTCCATC AAAACCTCAG
    GTTACAGATG TCAGTAAGAA CAGTGTTACC CTATCATGGC AACCAGGATC ACCAGGGATT
    CTGCCTGTGA CATCCTACAT CATTGAGGCT TTCAGTCAAT CTGTGAGCAA TAGTTGGCAA
    ACTGTGGCCA ATCACGTGAA GACAACGCTT TACACTGTTA AAGGACTGCG CCCAAACACC
    ATTTATCTCT TTATGGTGAG AGCAATCAAT ACTGAAGGAC TGAGTGACCC AAGTCCAATA
    TCTGATCCAG TGCGTACACA AGATATAAGT CCACCTGTTC AAGGAGTAGA TCACCGGCAA
    GTACAGAAAG AACTTGGGGA TGTGATTGTA CGGCTCCATA ATCCTGTTGT TCTGACACCA
    ACAACAATCC AGGTCACCTG GATGGTCGAC CGTCAATCCC AGTTTATTCA GGGATACAGG
    GTAATGTACC GTCAGACTTC TGGTCTGCAG ACTCCATCTG CGTGGCAGAG CTTAGAGGTC
    AAAGTGCCTG CAGAGCGTAG CGCAGTGCTA ACCAACCTGA AAAAAGGAAT TACTTATGAG
    ATTAAGGTTC GACCTTATTT TAATGAATTT CAAGGAATGG ATAGCGAGTC AAAGTCAGCA
    CGAACAACTG AAGAAGCCCC AAGTGCTCCT CCTCAGTCTG TTACTGTTAT GACAGTGGGA
    AATAACAACA GCACCAGCAT CAGCATCTCA TGGGACCCCC CTCCATCTGA CCACCAGAAT
    GGAATTATCC AGGACTACAG GATCTGGTGC CTGGGTAATG AGACACGTTT CCACATCAAC
    AAGACAGTGG ATGCTGCTAT TCGTTCCGTG GTTATCGGTG GGCTATTCCC TGGAATTCAG
    TACCGCGTTG AAGTCGCAGC CAGTACAAGC GCAGGCACTG GGGTAAAAAG TGCTCCCCAG
    CACATACAGA TAGGAAATCG GAATGAAGTT GTGATCACAG AGAATAACAA CAGCATAACA
    GAGCAAATAA CTGATGTTGT GAAACAGCCG GCATTCATCG CAGGAATTGG TGGTGCCTGC
    TGGGTAATAC TCATGGGATT TAGCATTTGG CTCTACTGGC GAAGAAAGAA GAGAAAGGGT
    CTCAGCAACT ACGCTGTTAC ATTCCAGAGA GGAGATGGAG GACTAATGAG TAATGGAAGT
    CGTCCTGGAC TTTTAAATGC GAATGATCCT AGTTACCCTT GGTTGGCAGA CTCCTGGCCA
    GCTTCCAGCA TGCCGGTGAA TAACAGCAAC AATGGACCAA ACGATCTCGT TAATTTTAGT
    TGTGGAGATG TGCTGCCAAC AGTTCCAGGT CAAGGAGAAA AAACAGCGAC TATGCTTTCT
    GATGGAGCCA TTTATAGCAG CATTGATTTT ACTACAAAAT CTAGCTATAA TTGCCCAGGA
    CAAATTGCAC AAACCACGCC ATATGCTACT ACGCAGATAC TACATTCCAA CAGCATTCAT
    GAGCTGGCAG TTGACTTGCC TGATCCACAG TGGAAATCTT CCATTCAGCA GAAATCTGAT
    CTCACTGGAT TAGGCTATTG CCTTACTGAC CAAGGCAAAG GGAGTAATGC CTTACTTTAC
    ATTCCTGACT ATCGAATGGC TGAGGGATTG TCAAATAGAA TACCCCACAA CCAGTCACAG
    GATTTCAGCA CCACCAGCTC TCACAACAGC TCAGAGAGAA GTGGCAGCCT TTCAGGTGGA
    AAAGACGGAA AAAGGAAGAA AAATAAAAAC AGCTCTAAAA TCTCTAAAAA CAATGGATCC
    AGTTGGGCCA ATGTTCCACT TCCGCCCCCT CCCATCCAAC CACTGCCAGG CACTGAAATA
    GAACATTATG GCCTTGAACA CCAGGAAACA GGCTATGACA CCAATGGATG GTGCCCACCT
    TTGCCAGTCC AGACCTACTT ACATCAAGGA TCAGATGATG AACTAGAAGA AGATGAAGAG
    AGAGTCCCTA CACCCCCTGT ACGTGGGGTG GCATCATCAC CTGCCATCTC ATTTGGACAA
    CAGTCAACAG CAACCCTCAC ACCATCACCA CGTGAAGAAA TGCAACCCAT GCTTCAGGCC
    CACCTGGATG AATTAACAAG AGCTTACCAG TTTGACATTG CAAAGCAATC TTGGCATTTA
    CACAGCAATA ACCAGCCCCC ATTGGCCCCA GTTCCACCAC TTGGATATGT GTCTGGGACA
    TTTATTTCTG ACTTAGAAAA AGACATTCCA GATCAAGAAG AAGATGAAAT TGAAGACGAA
    TCTATAGAAA TAACAAGGCC CCTCCGAGGT TTGGAACACA CTCCCGGTTC TAGTATGGAT
    AATCTCGACA GTTCTGTGAC AGGCAAAATG AACTCCTTTT CTCAGAGACC CCGGCCTGCC
    AGTCCCTTTT CTACTGACAG TAACACAAGT TCAGCTGTGA ATCATAGTCA GAAGTCACGG
    CCCACTAAGA AACACAAGGG AGGCAGAATA GGTCAGCAAC CCATGCTTCG CAGAGAAGGG
    ATTGCAGATG ATTTGCCTCC CCCACCTGAT CCTCCACCTT GTCAAAGACA GCAAATCATG
    CCCAGTCAAC GTGGTGGAGG TCCTACAGAA AGAAAAGCCA GTTCTCTTGA GAGGCACACA
    TCAAATATGA ATGATTTAAA AACCTCATTA GACCGGCATG GCAGAACATC CTTAGAACGG
    CAGCGACAAA CACAGGAATG GCTTGGCTCA ACAGAAAGGC AGAACAAGCA GGCATTTGGA
    TCTGAAGAGA TACTAATGCC ATACAGTAAA CCTAGCTTCC CATCTCCAGG AGGGCACAGC
    TCCTCAGGAA CAGTCTCTTC AAAAGAATCA ACTGGACCCA GAAAAGGGGA GGTTTTGAGA
    GGTCACCAAC GTAATGCCAT TGACTTTCTT GATGTAGGAT ATTTGGGGCC ACACAGTCAA
    GGACAGTTTA CAGGTGAATT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa51981
    Clone ID Related Accession (Same CDS sequence) XM_031896604.1
    Accession Version XM_031896604.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4572bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product roundabout homolog 2 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAACATCT GGATAATGCT ATCTTCACTA AGGCAGAGGG CAGCTTCTCC TGAATTCCTG 
    CTGATCCTTA TGCTGTTCGT CAGTGGGGTG ACCCAAAGCA AAATTAGTAG GACCAAGGGG
    AAGCACTGGA AAGGGGGATC CCGTCTCCGT CAAGAAGATT TCCCCCCACG GATAGTTGAG
    CATCCATCGG ATGTCATTGT GTCAAAGGGA GAGCCAACCA CCTTGAACTG CAAAGCGGAA
    GGGCGGCCTA CACCCACCAT AGAGTGGTAC AAAGACTTAG AAAGGGTTGA GACAGACAAG
    GATGACCCCC GCTCACATCG AATGCTACTG CCAAGTGGAT CTTTATTTTT TCTGCGCATT
    ATCCATGGGC GCAAGTTTAA GCCAGACGAA GGAAGTTACG TCTGTGTGGC AAGGAACTAT
    CTCGGGGAGG CTATGAGTCG AAATGCATCT CTGGAAGTGG CATTGCTCCG TGATGACTTC
    CGGCTCAACC CTACAGATGT TGTTGTGGCA GCTGGGGAAC CTGCAATTTT GGAGTGTCAA
    CCCCCCAGAG GACATCCGGA ACCAACTATA TACTGGAAAA AGGACAAAGT TAGAATTGAT
    GATAAAGATG AAAGAATTAG TATTCGAGGT GGAAAGCTGA TGCTCTCCAA TACAAGGAAA
    AGTGATGCAG GCATGTACAC TTGTGTTGGG ACCAACATGG TTGGAGAAAG AGACAGTGAC
    CCAGCAGAGC TAACAGTCTT TGAACGTCCC ACATTCCTCA GAAGGCCAAT TAATCAAGTT
    GTTTTAGAGG ATGAAGCTGT AGAATTTCGA TGTCAGGTCC AGGGGGACCC ACAGCCAACT
    GTCCGGTGGA AAAAGGATGA TGTAGATTTG CCAAGAGGAA GATACGACAT CAAGGATGAC
    TATACTTTGA GAATCAAGAA GGCTTCCAGC AGCGACGAAG GGACCTTTGC TTGTATTTCT
    GAAAACCGGG TTGGTAAAAT AGAAGCTTCT GCCACACTTA CAGTCAGAGC TCCTCCCCAG
    TTTGTGATCA GACCAAGGGA TCAGATTGTA GCACAAGGCC GGACAGTAAC ATTTGGATGT
    GAAGCTAAAG GAAATCCTCA ACCAGCTGTT TTTTGGCAAA AAGAAGGAAG CCAGAATTTA
    CTTTTTCCAA ATCAGCCTCT TCAGCCCAAC AGCAGGTACT CAGTGTCACC AACTGGAGAT
    TTAACCATCA CCAGCATTCA GCGCTCAGAC GCAGGCTACT ACATTTGTCA GGCACTGACT
    GTTGCCGGAA GCATTCTGGC CAAGGCCCAG CTGGAAGTGA CTGATGTTTT GACAGATAGA
    CCACCGCCAA TAATTCTACA AGGTCCAGCT AATCAAACTC TTGCCGTGGA TGGAACAGCA
    GTGCTGAAAT GTAAAACTAC TGGGGACCCA ATGCCAGTCA TAAGTTGGCT AAAAGAGGGA
    TTTAAGGGAT TCATAGGGAG AGATGCAAGG TTATCCATTC TAGACCAAGG GACATTACAA
    ATTAAAGGTT TAAAGGTTAC TGATTCTGGC ACCTATACTT GTGTGGCTAC TAGCTCTAGT
    GGTGAGACAA CATGGAGCGC TGTTCTGGAA GTGACAGAAT CTGGTGGTGC CATAATTAGC
    AAGAACTATG ATCTCAATGA CCTTCCTGGA TCTCCATCAA AACCTCAGGT TACAGATGTC
    AGTAAGAACA GTGTTACCCT ATCATGGCAA CCAGGATCAC CAGGGATTCT GCCTGTGACA
    TCCTACATCA TTGAGGCTTT CAGTCAATCT GTGAGCAATA GTTGGCAAAC TGTGGCCAAT
    CACGTGAAGA CAACGCTTTA CACTGTTAAA GGACTGCGCC CAAACACCAT TTATCTCTTT
    ATGGTGAGAG CAATCAATAC TGAAGGACTG AGTGACCCAA GTCCAATATC TGATCCAGTG
    CGTACACAAG ATATAAGTCC ACCTGTTCAA GGAGTAGATC ACCGGCAAGT ACAGAAAGAA
    CTTGGGGATG TGATTGTACG GCTCCATAAT CCTGTTGTTC TGACACCAAC AACAATCCAG
    GTCACCTGGA TGGTCGACCG TCAATCCCAG TTTATTCAGG GATACAGGGT AATGTACCGT
    CAGACTTCTG GTCTGCAGAC TCCATCTGCG TGGCAGAGCT TAGAGGTCAA AGTGCCTGCA
    GAGCGTAGCG CAGTGCTAAC CAACCTGAAA AAAGGAATTA CTTATGAGAT TAAGGTTCGA
    CCTTATTTTA ATGAATTTCA AGGAATGGAT AGCGAGTCAA AGTCAGCACG AACAACTGAA
    GAAGCCCCAA GTGCTCCTCC TCAGTCTGTT ACTGTTATGA CAGTGGGAAA TAACAACAGC
    ACCAGCATCA GCATCTCATG GGACCCCCCT CCATCTGACC ACCAGAATGG AATTATCCAG
    GACTACAGGA TCTGGTGCCT GGGTAATGAG ACACGTTTCC ACATCAACAA GACAGTGGAT
    GCTGCTATTC GTTCCGTGGT TATCGGTGGG CTATTCCCTG GAATTCAGTA CCGCGTTGAA
    GTCGCAGCCA GTACAAGCGC AGGCACTGGG GTAAAAAGTG CTCCCCAGCA CATACAGATA
    GGAAATCGGA ATGAAGTTGT GATCACAGAG AATAACAACA GCATAACAGA GCAAATAACT
    GATGTTGTGA AACAGCCGGC ATTCATCGCA GGAATTGGTG GTGCCTGCTG GGTAATACTC
    ATGGGATTTA GCATTTGGCT CTACTGGCGA AGAAAGAAGA GAAAGGGTCT CAGCAACTAC
    GCTGTTACAT TCCAGAGAGG AGATGGAGGA CTAATGAGTA ATGGAAGTCG TCCTGGACTT
    TTAAATGCGA ATGATCCTAG TTACCCTTGG TTGGCAGACT CCTGGCCAGC TTCCAGCATG
    CCGGTGAATA ACAGCAACAA TGGACCAAAC GATCTCGTTA ATTTTAGTTG TGGAGATGTG
    CTGCCAACAG TTCCAGGTCA AGGAGAAAAA ACAGCGACTA TGCTTTCTGA TGGAGCCATT
    TATAGCAGCA TTGATTTTAC TACAAAATCT AGCTATAATT GCCCAGGACA AATTGCACAA
    ACCACGCCAT ATGCTACTAC GCAGATACTA CATTCCAACA GCATTCATGA GCTGGCAGTT
    GACTTGCCTG ATCCACAGTG GAAATCTTCC ATTCAGCAGA AATCTGATCT CACTGGATTA
    GGCTATTGCC TTACTGACCA AGGCAAAGGG AGTAATGCCT TACTTTACAT TCCTGACTAT
    CGAATGGCTG AGGGATTGTC AAATAGAATA CCCCACAACC AGTCACAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAGAGAAGT GGCAGCCTTT CAGGTGGAAA AGACGGAAAA
    AGGAAGAAAA ATAAAAACAG CTCTAAAATC TCTAAAAACA ATGGATCCAG TTGGGCCAAT
    GTTCCACTTC CGCCCCCTCC CATCCAACCA CTGCCAGGCA CTGAAATAGA ACATTATGGC
    CTTGAACACC AGGAAACAGG CTATGACACC AATGGATGGT GCCCACCTTT GCCAGTCCAG
    ACCTACTTAC ATCAAGGATC AGATGATGAA CTAGAAGAAG ATGAAGAGAG AGTCCCTACA
    CCCCCTGTAC GTGGGGTGGC ATCATCACCT GCCATCTCAT TTGGACAACA GTCAACAGCA
    ACCCTCACAC CATCACCACG TGAAGAAATG CAACCCATGC TTCAGGCCCA CCTGGATGAA
    TTAACAAGAG CTTACCAGTT TGACATTGCA AAGCAATCTT GGCATTTACA CAGCAATAAC
    CAGCCCCCAT TGGCCCCAGT TCCACCACTT GGATATGTGT CTGGGACATT TATTTCTGAC
    TTAGAAAAAG ACATTCCAGA TCAAGAAGAA GATGAAATTG AAGACGAATC TATAGAAATA
    ACAAGGCCCC TCCGAGGTTT GGAACACACT CCCGGTTCTA GTATGGATAA TCTCGACAGT
    TCTGTGACAG GCAAAATGAA CTCCTTTTCT CAGAGACCCC GGCCTGCCAG TCCCTTTTCT
    ACTGACAGTA ACACAAGTTC AGCTGTGAAT CATAGTCAGA AGTCACGGCC CACTAAGAAA
    CACAAGGGAG GCAGAATAGG TCAGCAACCC ATGCTTCGCA GAGAAGGGAT TGCAGATGAT
    TTGCCTCCCC CACCTGATCC TCCACCTTGT CAAAGACAGC AAATCATGCC CAGTCAACGT
    GGTGGAGGTC CTACAGAAAG AAAAGCCAGT TCTCTTGAGA GGCACACATC AAATATGAAT
    GATTTAAAAA CCTCATTAGA CCGGCATGGC AGAACATCCT TAGAACGGCA GCGACAAACA
    CAGGAATGGC TTGGCTCAAC AGAAAGGCAG AACAAGCAGG CATTTGGATC TGAAGAGATA
    CTAATGCCAT ACAGTAAACC TAGCTTCCCA TCTCCAGGAG GGCACAGCTC CTCAGGAACA
    GTCTCTTCAA AAGAATCAAC TGGACCCAGA AAAGGGGAGG TTTTGAGAGG TCACCAACGT
    AATGCCATTG ACTTTCTTGA TGTAGGATAT TTGGGGCCAC ACAGTCAAGG ACAGTTTACA
    GGTGAATTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752464.1
    CDS541..5112
    Translation

    Target ORF information:

    RefSeq Version XM_031896604.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896604.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAACATCT GGATAATGCT ATCTTCACTA AGGCAGAGGG CAGCTTCTCC TGAATTCCTG 
    CTGATCCTTA TGCTGTTCGT CAGTGGGGTG ACCCAAAGCA AAATTAGTAG GACCAAGGGG
    AAGCACTGGA AAGGGGGATC CCGTCTCCGT CAAGAAGATT TCCCCCCACG GATAGTTGAG
    CATCCATCGG ATGTCATTGT GTCAAAGGGA GAGCCAACCA CCTTGAACTG CAAAGCGGAA
    GGGCGGCCTA CACCCACCAT AGAGTGGTAC AAAGACTTAG AAAGGGTTGA GACAGACAAG
    GATGACCCCC GCTCACATCG AATGCTACTG CCAAGTGGAT CTTTATTTTT TCTGCGCATT
    ATCCATGGGC GCAAGTTTAA GCCAGACGAA GGAAGTTACG TCTGTGTGGC AAGGAACTAT
    CTCGGGGAGG CTATGAGTCG AAATGCATCT CTGGAAGTGG CATTGCTCCG TGATGACTTC
    CGGCTCAACC CTACAGATGT TGTTGTGGCA GCTGGGGAAC CTGCAATTTT GGAGTGTCAA
    CCCCCCAGAG GACATCCGGA ACCAACTATA TACTGGAAAA AGGACAAAGT TAGAATTGAT
    GATAAAGATG AAAGAATTAG TATTCGAGGT GGAAAGCTGA TGCTCTCCAA TACAAGGAAA
    AGTGATGCAG GCATGTACAC TTGTGTTGGG ACCAACATGG TTGGAGAAAG AGACAGTGAC
    CCAGCAGAGC TAACAGTCTT TGAACGTCCC ACATTCCTCA GAAGGCCAAT TAATCAAGTT
    GTTTTAGAGG ATGAAGCTGT AGAATTTCGA TGTCAGGTCC AGGGGGACCC ACAGCCAACT
    GTCCGGTGGA AAAAGGATGA TGTAGATTTG CCAAGAGGAA GATACGACAT CAAGGATGAC
    TATACTTTGA GAATCAAGAA GGCTTCCAGC AGCGACGAAG GGACCTTTGC TTGTATTTCT
    GAAAACCGGG TTGGTAAAAT AGAAGCTTCT GCCACACTTA CAGTCAGAGC TCCTCCCCAG
    TTTGTGATCA GACCAAGGGA TCAGATTGTA GCACAAGGCC GGACAGTAAC ATTTGGATGT
    GAAGCTAAAG GAAATCCTCA ACCAGCTGTT TTTTGGCAAA AAGAAGGAAG CCAGAATTTA
    CTTTTTCCAA ATCAGCCTCT TCAGCCCAAC AGCAGGTACT CAGTGTCACC AACTGGAGAT
    TTAACCATCA CCAGCATTCA GCGCTCAGAC GCAGGCTACT ACATTTGTCA GGCACTGACT
    GTTGCCGGAA GCATTCTGGC CAAGGCCCAG CTGGAAGTGA CTGATGTTTT GACAGATAGA
    CCACCGCCAA TAATTCTACA AGGTCCAGCT AATCAAACTC TTGCCGTGGA TGGAACAGCA
    GTGCTGAAAT GTAAAACTAC TGGGGACCCA ATGCCAGTCA TAAGTTGGCT AAAAGAGGGA
    TTTAAGGGAT TCATAGGGAG AGATGCAAGG TTATCCATTC TAGACCAAGG GACATTACAA
    ATTAAAGGTT TAAAGGTTAC TGATTCTGGC ACCTATACTT GTGTGGCTAC TAGCTCTAGT
    GGTGAGACAA CATGGAGCGC TGTTCTGGAA GTGACAGAAT CTGGTGGTGC CATAATTAGC
    AAGAACTATG ATCTCAATGA CCTTCCTGGA TCTCCATCAA AACCTCAGGT TACAGATGTC
    AGTAAGAACA GTGTTACCCT ATCATGGCAA CCAGGATCAC CAGGGATTCT GCCTGTGACA
    TCCTACATCA TTGAGGCTTT CAGTCAATCT GTGAGCAATA GTTGGCAAAC TGTGGCCAAT
    CACGTGAAGA CAACGCTTTA CACTGTTAAA GGACTGCGCC CAAACACCAT TTATCTCTTT
    ATGGTGAGAG CAATCAATAC TGAAGGACTG AGTGACCCAA GTCCAATATC TGATCCAGTG
    CGTACACAAG ATATAAGTCC ACCTGTTCAA GGAGTAGATC ACCGGCAAGT ACAGAAAGAA
    CTTGGGGATG TGATTGTACG GCTCCATAAT CCTGTTGTTC TGACACCAAC AACAATCCAG
    GTCACCTGGA TGGTCGACCG TCAATCCCAG TTTATTCAGG GATACAGGGT AATGTACCGT
    CAGACTTCTG GTCTGCAGAC TCCATCTGCG TGGCAGAGCT TAGAGGTCAA AGTGCCTGCA
    GAGCGTAGCG CAGTGCTAAC CAACCTGAAA AAAGGAATTA CTTATGAGAT TAAGGTTCGA
    CCTTATTTTA ATGAATTTCA AGGAATGGAT AGCGAGTCAA AGTCAGCACG AACAACTGAA
    GAAGCCCCAA GTGCTCCTCC TCAGTCTGTT ACTGTTATGA CAGTGGGAAA TAACAACAGC
    ACCAGCATCA GCATCTCATG GGACCCCCCT CCATCTGACC ACCAGAATGG AATTATCCAG
    GACTACAGGA TCTGGTGCCT GGGTAATGAG ACACGTTTCC ACATCAACAA GACAGTGGAT
    GCTGCTATTC GTTCCGTGGT TATCGGTGGG CTATTCCCTG GAATTCAGTA CCGCGTTGAA
    GTCGCAGCCA GTACAAGCGC AGGCACTGGG GTAAAAAGTG CTCCCCAGCA CATACAGATA
    GGAAATCGGA ATGAAGTTGT GATCACAGAG AATAACAACA GCATAACAGA GCAAATAACT
    GATGTTGTGA AACAGCCGGC ATTCATCGCA GGAATTGGTG GTGCCTGCTG GGTAATACTC
    ATGGGATTTA GCATTTGGCT CTACTGGCGA AGAAAGAAGA GAAAGGGTCT CAGCAACTAC
    GCTGTTACAT TCCAGAGAGG AGATGGAGGA CTAATGAGTA ATGGAAGTCG TCCTGGACTT
    TTAAATGCGA ATGATCCTAG TTACCCTTGG TTGGCAGACT CCTGGCCAGC TTCCAGCATG
    CCGGTGAATA ACAGCAACAA TGGACCAAAC GATCTCGTTA ATTTTAGTTG TGGAGATGTG
    CTGCCAACAG TTCCAGGTCA AGGAGAAAAA ACAGCGACTA TGCTTTCTGA TGGAGCCATT
    TATAGCAGCA TTGATTTTAC TACAAAATCT AGCTATAATT GCCCAGGACA AATTGCACAA
    ACCACGCCAT ATGCTACTAC GCAGATACTA CATTCCAACA GCATTCATGA GCTGGCAGTT
    GACTTGCCTG ATCCACAGTG GAAATCTTCC ATTCAGCAGA AATCTGATCT CACTGGATTA
    GGCTATTGCC TTACTGACCA AGGCAAAGGG AGTAATGCCT TACTTTACAT TCCTGACTAT
    CGAATGGCTG AGGGATTGTC AAATAGAATA CCCCACAACC AGTCACAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAGAGAAGT GGCAGCCTTT CAGGTGGAAA AGACGGAAAA
    AGGAAGAAAA ATAAAAACAG CTCTAAAATC TCTAAAAACA ATGGATCCAG TTGGGCCAAT
    GTTCCACTTC CGCCCCCTCC CATCCAACCA CTGCCAGGCA CTGAAATAGA ACATTATGGC
    CTTGAACACC AGGAAACAGG CTATGACACC AATGGATGGT GCCCACCTTT GCCAGTCCAG
    ACCTACTTAC ATCAAGGATC AGATGATGAA CTAGAAGAAG ATGAAGAGAG AGTCCCTACA
    CCCCCTGTAC GTGGGGTGGC ATCATCACCT GCCATCTCAT TTGGACAACA GTCAACAGCA
    ACCCTCACAC CATCACCACG TGAAGAAATG CAACCCATGC TTCAGGCCCA CCTGGATGAA
    TTAACAAGAG CTTACCAGTT TGACATTGCA AAGCAATCTT GGCATTTACA CAGCAATAAC
    CAGCCCCCAT TGGCCCCAGT TCCACCACTT GGATATGTGT CTGGGACATT TATTTCTGAC
    TTAGAAAAAG ACATTCCAGA TCAAGAAGAA GATGAAATTG AAGACGAATC TATAGAAATA
    ACAAGGCCCC TCCGAGGTTT GGAACACACT CCCGGTTCTA GTATGGATAA TCTCGACAGT
    TCTGTGACAG GCAAAATGAA CTCCTTTTCT CAGAGACCCC GGCCTGCCAG TCCCTTTTCT
    ACTGACAGTA ACACAAGTTC AGCTGTGAAT CATAGTCAGA AGTCACGGCC CACTAAGAAA
    CACAAGGGAG GCAGAATAGG TCAGCAACCC ATGCTTCGCA GAGAAGGGAT TGCAGATGAT
    TTGCCTCCCC CACCTGATCC TCCACCTTGT CAAAGACAGC AAATCATGCC CAGTCAACGT
    GGTGGAGGTC CTACAGAAAG AAAAGCCAGT TCTCTTGAGA GGCACACATC AAATATGAAT
    GATTTAAAAA CCTCATTAGA CCGGCATGGC AGAACATCCT TAGAACGGCA GCGACAAACA
    CAGGAATGGC TTGGCTCAAC AGAAAGGCAG AACAAGCAGG CATTTGGATC TGAAGAGATA
    CTAATGCCAT ACAGTAAACC TAGCTTCCCA TCTCCAGGAG GGCACAGCTC CTCAGGAACA
    GTCTCTTCAA AAGAATCAAC TGGACCCAGA AAAGGGGAGG TTTTGAGAGG TCACCAACGT
    AATGCCATTG ACTTTCTTGA TGTAGGATAT TTGGGGCCAC ACAGTCAAGG ACAGTTTACA
    GGTGAATTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa06334
    Clone ID Related Accession (Same CDS sequence) XM_004912079.3
    Accession Version XM_004912079.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4599bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product roundabout homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004912079.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGCTTCC TGGTGGTGCC CCTGCTGTGT GGATTGTTAT GCTGTCAGGT GTGGGGATCC 
    CGTCTCCGTC AAGAAGATTT CCCCCCACGG ATAGTTGAGC ATCCATCGGA TGTCATTGTG
    TCAAAGGGAG AGCCAACCAC CTTGAACTGC AAAGCGGAAG GGCGGCCTAC ACCCACCATA
    GAGTGGTACA AAGACTTAGA AAGGGTTGAG ACAGACAAGG ATGACCCCCG CTCACATCGA
    ATGCTACTGC CAAGTGGATC TTTATTTTTT CTGCGCATTA TCCATGGGCG CAAGTTTAAG
    CCAGACGAAG GAAGTTACGT CTGTGTGGCA AGGAACTATC TCGGGGAGGC TATGAGTCGA
    AATGCATCTC TGGAAGTGGC ATTGCTCCGT GATGACTTCC GGCTCAACCC TACAGATGTT
    GTTGTGGCAG CTGGGGAACC TGCAATTTTG GAGTGTCAAC CCCCCAGAGG ACATCCGGAA
    CCAACTATAT ACTGGAAAAA GGACAAAGTT AGAATTGATG ATAAAGATGA AAGAATTAGT
    ATTCGAGGTG GAAAGCTGAT GCTCTCCAAT ACAAGGAAAA GTGATGCAGG CATGTACACT
    TGTGTTGGGA CCAACATGGT TGGAGAAAGA GACAGTGACC CAGCAGAGCT AACAGTCTTT
    GAACGTCCCA CATTCCTCAG AAGGCCAATT AATCAAGTTG TTTTAGAGGA TGAAGCTGTA
    GAATTTCGAT GTCAGGTCCA GGGGGACCCA CAGCCAACTG TCCGGTGGAA AAAGGATGAT
    GTAGATTTGC CAAGAGGAAG ATACGACATC AAGGATGACT ATACTTTGAG AATCAAGAAG
    GCTTCCAGCA GCGACGAAGG GACCTTTGCT TGTATTTCTG AAAACCGGGT TGGTAAAATA
    GAAGCTTCTG CCACACTTAC AGTCAGAGTT CGCCCTGTTG CTCCTCCCCA GTTTGTGATC
    AGACCAAGGG ATCAGATTGT AGCACAAGGC CGGACAGTAA CATTTGGATG TGAAGCTAAA
    GGAAATCCTC AACCAGCTGT TTTTTGGCAA AAAGAAGGAA GCCAGAATTT ACTTTTTCCA
    AATCAGCCTC TTCAGCCCAA CAGCAGGTAC TCAGTGTCAC CAACTGGAGA TTTAACCATC
    ACCAGCATTC AGCGCTCAGA CGCAGGCTAC TACATTTGTC AGGCACTGAC TGTTGCCGGA
    AGCATTCTGG CCAAGGCCCA GCTGGAAGTG ACTGATGTTT TGACAGATAG ACCACCGCCA
    ATAATTCTAC AAGGTCCAGC TAATCAAACT CTTGCCGTGG ATGGAACAGC AGTGCTGAAA
    TGTAAAACTA CTGGGGACCC AATGCCAGTC ATAAGTTGGC TAAAAGAGGG ATTTAAGGGA
    TTCATAGGGA GAGATGCAAG GTTATCCATT CTAGACCAAG GGACATTACA AATTAAAGGT
    TTAAAGGTTA CTGATTCTGG CACCTATACT TGTGTGGCTA CTAGCTCTAG TGGTGAGACA
    ACATGGAGCG CTGTTCTGGA AGTGACAGAA TCTGGTGGTG CCATAATTAG CAAGAACTAT
    GATCTCAATG ACCTTCCTGG ATCTCCATCA AAACCTCAGG TTACAGATGT CAGTAAGAAC
    AGTGTTACCC TATCATGGCA ACCAGGATCA CCAGGGATTC TGCCTGTGAC ATCCTACATC
    ATTGAGGCTT TCAGTCAATC TGTGAGCAAT AGTTGGCAAA CTGTGGCCAA TCACGTGAAG
    ACAACGCTTT ACACTGTTAA AGGACTGCGC CCAAACACCA TTTATCTCTT TATGGTGAGA
    GCAATCAATA CTGAAGGACT GAGTGACCCA AGTCCAATAT CTGATCCAGT GCGTACACAA
    GATATAAGTC CACCTGTTCA AGGAGTAGAT CACCGACAAG TACAGAAAGA ACTTGGGGAT
    GTGATTGTAC GGCTCCATAA TCCTGTTGTT CTGACACCAA CAACAATCCA GGTCACCTGG
    ATGGTCGACC GTCAATCCCA GTTTATTCAG GGATACAGGG TAATGTACCG TCAGACTTCT
    GGTCTGCAGA CTCCATCTGC GTGGCAGAGC TTAGAGGTCA AAGTGCCTGC AGAGCGTAGC
    GCAGTGCTAA CCAACCTGAA AAAAGGAATT ACTTATGAGA TTAAGGTTCG ACCTTATTTT
    AATGAATTTC AAGGAATGGA TAGCGAGTCA AAGTCAGCAC GAACAACTGA AGAAGCCCCA
    AGTGCTCCTC CTCAGTCTGT TACTGTTATG ACAGTGGGAA ATAACAACAG CACCAGCATC
    AGCATCTCAT GGGACCCCCC TCCATCTGAC CACCAGAATG GAATTATCCA GGACTACAGG
    ATCTGGTGCC TGGGTAATGA GACACGTTTC CACATCAACA AGACAGTGGA TGCTGCTATT
    CGTTCCGTGG TTATCGGTGG GCTATTCCCT GGAATTCAGT ACCGCGTTGA AGTCGCAGCC
    AGTACAAGCG CAGGCACTGG GGTAAAAAGT GCTCCCCAGC ACATACAGAT AGGAAATCGG
    AATGAAGTTG TGATCACAGA GAATAACAAC AGCATAACAG AGCAAATAAC TGATGTTGTG
    AAACAGCCGG CATTCATCGC AGGAATTGGT GGTGCCTGCT GGGTAATACT CATGGGATTT
    AGCATTTGGC TCTACTGGCG AAGAAAGAAG AGAAAGGGTC TCAGCAACTA CGCTGTTCAG
    TCTTTCACCT TTACCCCTGC AGATTCCCCA GATACAACCA TGCACCACAA GGTTGCACTT
    GGCACAAAGA AGGGGAGCTG GCACAAAACA GTTACATTCC AGAGAGGAGA TGGAGGACTA
    ATGAGTAATG GAAGTCGTCC TGGACTTTTA AATGCGAATG ATCCTAGTTA CCCTTGGTTG
    GCAGACTCCT GGCCAGCTTC CAGCATGCCG GTGAATAACA GCAACAATGG ACCAAACGAT
    CTCGTTAATT TTAGTTGTGG AGATGTGCTG CCAACAGTTC CAGGTCAAGG AGAAAAAACA
    GCGACTATGC TTTCTGATGG AGCCATTTAT AGCAGCATTG ATTTTACTAC AAAATCTAGC
    TATAATTGCC CAGGACAAAT TGCACAAACC ACGCCATATG CTACTACGCA GATACTACAT
    TCCAACAGCA TTCATGAGCT GGCAGTTGAC TTGCCTGATC CACAGTGGAA ATCTTCCATT
    CAGCAGAAAT CTGATCTCAC TGGATTAGGC TATTGCCTTA CTGACCAAGG CAAAGGGAGT
    AATGCCTTAC TTTACATTCC TGACTATCGA ATGGCTGAGG GATTGTCAAA TAGAATACCC
    CACAACCAGT CACAGGATTT CAGCACCACC AGCTCTCACA ACAGCTCAGA GAGAAGTGGC
    AGCCTTTCAG GTGGAAAAGA CGGAAAAAGG AAGAAAAATA AAAACAGCTC TAAAATCTCT
    AAAAACAATG GATCCAGTTG GGCCAATGTT CCACTTCCGC CCCCTCCCAT CCAACCACTG
    CCAGGCACTG AAATAGAACA TTATGGCCTT GAACACCAGG AAACAGGCTA TGACACCAAT
    GGATGGTGCC CACCTTTGCC AGTCCAGACC TACTTACATC AAGGATCAGA TGATGAACTA
    GAAGAAGATG AAGAGAGAGT CCCTACACCC CCTGTACGTG GGGTGGCATC ATCACCTGCC
    ATCTCATTTG GACAACAGTC AACAGCAACC CTCACACCAT CACCACGTGA AGAAATGCAA
    CCCATGCTTC AGGCCCACCT GGATGAATTA ACAAGAGCTT ACCAGTTTGA CATTGCAAAG
    CAATCTTGGC ATTTACACAG CAATAACCAG CCCCCATTGG CCCCAGTTCC ACCACTTGGA
    TATGTGTCTG GGACATTTAT TTCTGACTTA GAAAAAGACA TTCCAGATCA AGAAGAAGAT
    GAAATTGAAG ACGAATCTAT AGAAATAACA AGGCCCCTCC GAGGTTTGGA ACACACTCCC
    GGTTCTAGTA TGGATAATCT CGACAGTTCT GTGACAGGCA AAATGAACTC CTTTTCTCAG
    AGACCCCGGC CTGCCAGTCC CTTTTCTACT GACAGTAACA CAAGTTCAGC TGTGAATCAT
    AGTCAGAAGT CACGGCCCAC TAAGAAACAC AAGGGAGGCA GAATAGATCA GCAACCCATG
    CTTCGCAGAG AAGGGATTGC AGATGATTTG CCTCCCCCAC CTGATCCTCC ACCTTGTCAA
    AGACAGCAAA TCATGCCCAG TCAACGTGGT GGAGGTCCTA CAGAAAGAAA AGCCAGTTCT
    CTTGAGAGGC ACACATCAAA TATGAATGAT TTAAAAACCT CATTAGACCG GCATGGCAGA
    ACATCCTTAG AACGGCAGCG ACAAACACAG GAATGGCTTG GCTCGACAGA AAGGCAGAAC
    AAGCAGGCAT TTGGATCTGA AGAGATACTA ATGCCATACA GTAAACCTAG CTTCCCATCT
    CCAGGAGGGC ACAGCTCCTC AGGAACAGTC TCTTCAAAAG AATCAACTGG ACCCAGAAAA
    GGGGAGGTTT TGAGAGGTCA CCAACGTAAT GCCATTGACT TTCTTGATGT AGGATATTTG
    GGGCCACACA GTCAAGGACA GTTTACAGGT GAATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004912136.1
    CDS755..5353
    Translation

    Target ORF information:

    RefSeq Version XM_004912079.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004912079.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGCTTCC TGGTGGTGCC CCTGCTGTGT GGATTGTTAT GCTGTCAGGT GTGGGGATCC 
    CGTCTCCGTC AAGAAGATTT CCCCCCACGG ATAGTTGAGC ATCCATCGGA TGTCATTGTG
    TCAAAGGGAG AGCCAACCAC CTTGAACTGC AAAGCGGAAG GGCGGCCTAC ACCCACCATA
    GAGTGGTACA AAGACTTAGA AAGGGTTGAG ACAGACAAGG ATGACCCCCG CTCACATCGA
    ATGCTACTGC CAAGTGGATC TTTATTTTTT CTGCGCATTA TCCATGGGCG CAAGTTTAAG
    CCAGACGAAG GAAGTTACGT CTGTGTGGCA AGGAACTATC TCGGGGAGGC TATGAGTCGA
    AATGCATCTC TGGAAGTGGC ATTGCTCCGT GATGACTTCC GGCTCAACCC TACAGATGTT
    GTTGTGGCAG CTGGGGAACC TGCAATTTTG GAGTGTCAAC CCCCCAGAGG ACATCCGGAA
    CCAACTATAT ACTGGAAAAA GGACAAAGTT AGAATTGATG ATAAAGATGA AAGAATTAGT
    ATTCGAGGTG GAAAGCTGAT GCTCTCCAAT ACAAGGAAAA GTGATGCAGG CATGTACACT
    TGTGTTGGGA CCAACATGGT TGGAGAAAGA GACAGTGACC CAGCAGAGCT AACAGTCTTT
    GAACGTCCCA CATTCCTCAG AAGGCCAATT AATCAAGTTG TTTTAGAGGA TGAAGCTGTA
    GAATTTCGAT GTCAGGTCCA GGGGGACCCA CAGCCAACTG TCCGGTGGAA AAAGGATGAT
    GTAGATTTGC CAAGAGGAAG ATACGACATC AAGGATGACT ATACTTTGAG AATCAAGAAG
    GCTTCCAGCA GCGACGAAGG GACCTTTGCT TGTATTTCTG AAAACCGGGT TGGTAAAATA
    GAAGCTTCTG CCACACTTAC AGTCAGAGTT CGCCCTGTTG CTCCTCCCCA GTTTGTGATC
    AGACCAAGGG ATCAGATTGT AGCACAAGGC CGGACAGTAA CATTTGGATG TGAAGCTAAA
    GGAAATCCTC AACCAGCTGT TTTTTGGCAA AAAGAAGGAA GCCAGAATTT ACTTTTTCCA
    AATCAGCCTC TTCAGCCCAA CAGCAGGTAC TCAGTGTCAC CAACTGGAGA TTTAACCATC
    ACCAGCATTC AGCGCTCAGA CGCAGGCTAC TACATTTGTC AGGCACTGAC TGTTGCCGGA
    AGCATTCTGG CCAAGGCCCA GCTGGAAGTG ACTGATGTTT TGACAGATAG ACCACCGCCA
    ATAATTCTAC AAGGTCCAGC TAATCAAACT CTTGCCGTGG ATGGAACAGC AGTGCTGAAA
    TGTAAAACTA CTGGGGACCC AATGCCAGTC ATAAGTTGGC TAAAAGAGGG ATTTAAGGGA
    TTCATAGGGA GAGATGCAAG GTTATCCATT CTAGACCAAG GGACATTACA AATTAAAGGT
    TTAAAGGTTA CTGATTCTGG CACCTATACT TGTGTGGCTA CTAGCTCTAG TGGTGAGACA
    ACATGGAGCG CTGTTCTGGA AGTGACAGAA TCTGGTGGTG CCATAATTAG CAAGAACTAT
    GATCTCAATG ACCTTCCTGG ATCTCCATCA AAACCTCAGG TTACAGATGT CAGTAAGAAC
    AGTGTTACCC TATCATGGCA ACCAGGATCA CCAGGGATTC TGCCTGTGAC ATCCTACATC
    ATTGAGGCTT TCAGTCAATC TGTGAGCAAT AGTTGGCAAA CTGTGGCCAA TCACGTGAAG
    ACAACGCTTT ACACTGTTAA AGGACTGCGC CCAAACACCA TTTATCTCTT TATGGTGAGA
    GCAATCAATA CTGAAGGACT GAGTGACCCA AGTCCAATAT CTGATCCAGT GCGTACACAA
    GATATAAGTC CACCTGTTCA AGGAGTAGAT CACCGACAAG TACAGAAAGA ACTTGGGGAT
    GTGATTGTAC GGCTCCATAA TCCTGTTGTT CTGACACCAA CAACAATCCA GGTCACCTGG
    ATGGTCGACC GTCAATCCCA GTTTATTCAG GGATACAGGG TAATGTACCG TCAGACTTCT
    GGTCTGCAGA CTCCATCTGC GTGGCAGAGC TTAGAGGTCA AAGTGCCTGC AGAGCGTAGC
    GCAGTGCTAA CCAACCTGAA AAAAGGAATT ACTTATGAGA TTAAGGTTCG ACCTTATTTT
    AATGAATTTC AAGGAATGGA TAGCGAGTCA AAGTCAGCAC GAACAACTGA AGAAGCCCCA
    AGTGCTCCTC CTCAGTCTGT TACTGTTATG ACAGTGGGAA ATAACAACAG CACCAGCATC
    AGCATCTCAT GGGACCCCCC TCCATCTGAC CACCAGAATG GAATTATCCA GGACTACAGG
    ATCTGGTGCC TGGGTAATGA GACACGTTTC CACATCAACA AGACAGTGGA TGCTGCTATT
    CGTTCCGTGG TTATCGGTGG GCTATTCCCT GGAATTCAGT ACCGCGTTGA AGTCGCAGCC
    AGTACAAGCG CAGGCACTGG GGTAAAAAGT GCTCCCCAGC ACATACAGAT AGGAAATCGG
    AATGAAGTTG TGATCACAGA GAATAACAAC AGCATAACAG AGCAAATAAC TGATGTTGTG
    AAACAGCCGG CATTCATCGC AGGAATTGGT GGTGCCTGCT GGGTAATACT CATGGGATTT
    AGCATTTGGC TCTACTGGCG AAGAAAGAAG AGAAAGGGTC TCAGCAACTA CGCTGTTCAG
    TCTTTCACCT TTACCCCTGC AGATTCCCCA GATACAACCA TGCACCACAA GGTTGCACTT
    GGCACAAAGA AGGGGAGCTG GCACAAAACA GTTACATTCC AGAGAGGAGA TGGAGGACTA
    ATGAGTAATG GAAGTCGTCC TGGACTTTTA AATGCGAATG ATCCTAGTTA CCCTTGGTTG
    GCAGACTCCT GGCCAGCTTC CAGCATGCCG GTGAATAACA GCAACAATGG ACCAAACGAT
    CTCGTTAATT TTAGTTGTGG AGATGTGCTG CCAACAGTTC CAGGTCAAGG AGAAAAAACA
    GCGACTATGC TTTCTGATGG AGCCATTTAT AGCAGCATTG ATTTTACTAC AAAATCTAGC
    TATAATTGCC CAGGACAAAT TGCACAAACC ACGCCATATG CTACTACGCA GATACTACAT
    TCCAACAGCA TTCATGAGCT GGCAGTTGAC TTGCCTGATC CACAGTGGAA ATCTTCCATT
    CAGCAGAAAT CTGATCTCAC TGGATTAGGC TATTGCCTTA CTGACCAAGG CAAAGGGAGT
    AATGCCTTAC TTTACATTCC TGACTATCGA ATGGCTGAGG GATTGTCAAA TAGAATACCC
    CACAACCAGT CACAGGATTT CAGCACCACC AGCTCTCACA ACAGCTCAGA GAGAAGTGGC
    AGCCTTTCAG GTGGAAAAGA CGGAAAAAGG AAGAAAAATA AAAACAGCTC TAAAATCTCT
    AAAAACAATG GATCCAGTTG GGCCAATGTT CCACTTCCGC CCCCTCCCAT CCAACCACTG
    CCAGGCACTG AAATAGAACA TTATGGCCTT GAACACCAGG AAACAGGCTA TGACACCAAT
    GGATGGTGCC CACCTTTGCC AGTCCAGACC TACTTACATC AAGGATCAGA TGATGAACTA
    GAAGAAGATG AAGAGAGAGT CCCTACACCC CCTGTACGTG GGGTGGCATC ATCACCTGCC
    ATCTCATTTG GACAACAGTC AACAGCAACC CTCACACCAT CACCACGTGA AGAAATGCAA
    CCCATGCTTC AGGCCCACCT GGATGAATTA ACAAGAGCTT ACCAGTTTGA CATTGCAAAG
    CAATCTTGGC ATTTACACAG CAATAACCAG CCCCCATTGG CCCCAGTTCC ACCACTTGGA
    TATGTGTCTG GGACATTTAT TTCTGACTTA GAAAAAGACA TTCCAGATCA AGAAGAAGAT
    GAAATTGAAG ACGAATCTAT AGAAATAACA AGGCCCCTCC GAGGTTTGGA ACACACTCCC
    GGTTCTAGTA TGGATAATCT CGACAGTTCT GTGACAGGCA AAATGAACTC CTTTTCTCAG
    AGACCCCGGC CTGCCAGTCC CTTTTCTACT GACAGTAACA CAAGTTCAGC TGTGAATCAT
    AGTCAGAAGT CACGGCCCAC TAAGAAACAC AAGGGAGGCA GAATAGATCA GCAACCCATG
    CTTCGCAGAG AAGGGATTGC AGATGATTTG CCTCCCCCAC CTGATCCTCC ACCTTGTCAA
    AGACAGCAAA TCATGCCCAG TCAACGTGGT GGAGGTCCTA CAGAAAGAAA AGCCAGTTCT
    CTTGAGAGGC ACACATCAAA TATGAATGAT TTAAAAACCT CATTAGACCG GCATGGCAGA
    ACATCCTTAG AACGGCAGCG ACAAACACAG GAATGGCTTG GCTCGACAGA AAGGCAGAAC
    AAGCAGGCAT TTGGATCTGA AGAGATACTA ATGCCATACA GTAAACCTAG CTTCCCATCT
    CCAGGAGGGC ACAGCTCCTC AGGAACAGTC TCTTCAAAAG AATCAACTGG ACCCAGAAAA
    GGGGAGGTTT TGAGAGGTCA CCAACGTAAT GCCATTGACT TTCTTGATGT AGGATATTTG
    GGGCCACACA GTCAAGGACA GTTTACAGGT GAATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa06335
    Clone ID Related Accession (Same CDS sequence) XM_004912080.3
    Accession Version XM_004912080.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4503bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product roundabout homolog 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004912080.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGCTTCC TGGTGGTGCC CCTGCTGTGT GGATTGTTAT GCTGTCAGGT GTGGGGATCC 
    CGTCTCCGTC AAGAAGATTT CCCCCCACGG ATAGTTGAGC ATCCATCGGA TGTCATTGTG
    TCAAAGGGAG AGCCAACCAC CTTGAACTGC AAAGCGGAAG GGCGGCCTAC ACCCACCATA
    GAGTGGTACA AAGACTTAGA AAGGGTTGAG ACAGACAAGG ATGACCCCCG CTCACATCGA
    ATGCTACTGC CAAGTGGATC TTTATTTTTT CTGCGCATTA TCCATGGGCG CAAGTTTAAG
    CCAGACGAAG GAAGTTACGT CTGTGTGGCA AGGAACTATC TCGGGGAGGC TATGAGTCGA
    AATGCATCTC TGGAAGTGGC ATTGCTCCGT GATGACTTCC GGCTCAACCC TACAGATGTT
    GTTGTGGCAG CTGGGGAACC TGCAATTTTG GAGTGTCAAC CCCCCAGAGG ACATCCGGAA
    CCAACTATAT ACTGGAAAAA GGACAAAGTT AGAATTGATG ATAAAGATGA AAGAATTAGT
    ATTCGAGGTG GAAAGCTGAT GCTCTCCAAT ACAAGGAAAA GTGATGCAGG CATGTACACT
    TGTGTTGGGA CCAACATGGT TGGAGAAAGA GACAGTGACC CAGCAGAGCT AACAGTCTTT
    GAACGTCCCA CATTCCTCAG AAGGCCAATT AATCAAGTTG TTTTAGAGGA TGAAGCTGTA
    GAATTTCGAT GTCAGGTCCA GGGGGACCCA CAGCCAACTG TCCGGTGGAA AAAGGATGAT
    GTAGATTTGC CAAGAGGAAG ATACGACATC AAGGATGACT ATACTTTGAG AATCAAGAAG
    GCTTCCAGCA GCGACGAAGG GACCTTTGCT TGTATTTCTG AAAACCGGGT TGGTAAAATA
    GAAGCTTCTG CCACACTTAC AGTCAGAGTT CGCCCTGTTG CTCCTCCCCA GTTTGTGATC
    AGACCAAGGG ATCAGATTGT AGCACAAGGC CGGACAGTAA CATTTGGATG TGAAGCTAAA
    GGAAATCCTC AACCAGCTGT TTTTTGGCAA AAAGAAGGAA GCCAGAATTT ACTTTTTCCA
    AATCAGCCTC TTCAGCCCAA CAGCAGGTAC TCAGTGTCAC CAACTGGAGA TTTAACCATC
    ACCAGCATTC AGCGCTCAGA CGCAGGCTAC TACATTTGTC AGGCACTGAC TGTTGCCGGA
    AGCATTCTGG CCAAGGCCCA GCTGGAAGTG ACTGATGTTT TGACAGATAG ACCACCGCCA
    ATAATTCTAC AAGGTCCAGC TAATCAAACT CTTGCCGTGG ATGGAACAGC AGTGCTGAAA
    TGTAAAACTA CTGGGGACCC AATGCCAGTC ATAAGTTGGC TAAAAGAGGG ATTTAAGGGA
    TTCATAGGGA GAGATGCAAG GTTATCCATT CTAGACCAAG GGACATTACA AATTAAAGGT
    TTAAAGGTTA CTGATTCTGG CACCTATACT TGTGTGGCTA CTAGCTCTAG TGGTGAGACA
    ACATGGAGCG CTGTTCTGGA AGTGACAGAA TCTGGTGGTG CCATAATTAG CAAGAACTAT
    GATCTCAATG ACCTTCCTGG ATCTCCATCA AAACCTCAGG TTACAGATGT CAGTAAGAAC
    AGTGTTACCC TATCATGGCA ACCAGGATCA CCAGGGATTC TGCCTGTGAC ATCCTACATC
    ATTGAGGCTT TCAGTCAATC TGTGAGCAAT AGTTGGCAAA CTGTGGCCAA TCACGTGAAG
    ACAACGCTTT ACACTGTTAA AGGACTGCGC CCAAACACCA TTTATCTCTT TATGGTGAGA
    GCAATCAATA CTGAAGGACT GAGTGACCCA AGTCCAATAT CTGATCCAGT GCGTACACAA
    GATATAAGTC CACCTGTTCA AGGAGTAGAT CACCGACAAG TACAGAAAGA ACTTGGGGAT
    GTGATTGTAC GGCTCCATAA TCCTGTTGTT CTGACACCAA CAACAATCCA GGTCACCTGG
    ATGGTCGACC GTCAATCCCA GTTTATTCAG GGATACAGGG TAATGTACCG TCAGACTTCT
    GGTCTGCAGA CTCCATCTGC GTGGCAGAGC TTAGAGGTCA AAGTGCCTGC AGAGCGTAGC
    GCAGTGCTAA CCAACCTGAA AAAAGGAATT ACTTATGAGA TTAAGGTTCG ACCTTATTTT
    AATGAATTTC AAGGAATGGA TAGCGAGTCA AAGTCAGCAC GAACAACTGA AGAAGCCCCA
    AGTGCTCCTC CTCAGTCTGT TACTGTTATG ACAGTGGGAA ATAACAACAG CACCAGCATC
    AGCATCTCAT GGGACCCCCC TCCATCTGAC CACCAGAATG GAATTATCCA GGACTACAGG
    ATCTGGTGCC TGGGTAATGA GACACGTTTC CACATCAACA AGACAGTGGA TGCTGCTATT
    CGTTCCGTGG TTATCGGTGG GCTATTCCCT GGAATTCAGT ACCGCGTTGA AGTCGCAGCC
    AGTACAAGCG CAGGCACTGG GGTAAAAAGT GCTCCCCAGC ACATACAGAT AGGAAATCGG
    AATGAAGTTG TGATCACAGA GAATAACAAC AGCATAACAG AGCAAATAAC TGATGTTGTG
    AAACAGCCGG CATTCATCGC AGGAATTGGT GGTGCCTGCT GGGTAATACT CATGGGATTT
    AGCATTTGGC TCTACTGGCG AAGAAAGAAG AGAAAGGGTC TCAGCAACTA CGCTGTTACA
    TTCCAGAGAG GAGATGGAGG ACTAATGAGT AATGGAAGTC GTCCTGGACT TTTAAATGCG
    AATGATCCTA GTTACCCTTG GTTGGCAGAC TCCTGGCCAG CTTCCAGCAT GCCGGTGAAT
    AACAGCAACA ATGGACCAAA CGATCTCGTT AATTTTAGTT GTGGAGATGT GCTGCCAACA
    GTTCCAGGTC AAGGAGAAAA AACAGCGACT ATGCTTTCTG ATGGAGCCAT TTATAGCAGC
    ATTGATTTTA CTACAAAATC TAGCTATAAT TGCCCAGGAC AAATTGCACA AACCACGCCA
    TATGCTACTA CGCAGATACT ACATTCCAAC AGCATTCATG AGCTGGCAGT TGACTTGCCT
    GATCCACAGT GGAAATCTTC CATTCAGCAG AAATCTGATC TCACTGGATT AGGCTATTGC
    CTTACTGACC AAGGCAAAGG GAGTAATGCC TTACTTTACA TTCCTGACTA TCGAATGGCT
    GAGGGATTGT CAAATAGAAT ACCCCACAAC CAGTCACAGG ATTTCAGCAC CACCAGCTCT
    CACAACAGCT CAGAGAGAAG TGGCAGCCTT TCAGGTGGAA AAGACGGAAA AAGGAAGAAA
    AATAAAAACA GCTCTAAAAT CTCTAAAAAC AATGGATCCA GTTGGGCCAA TGTTCCACTT
    CCGCCCCCTC CCATCCAACC ACTGCCAGGC ACTGAAATAG AACATTATGG CCTTGAACAC
    CAGGAAACAG GCTATGACAC CAATGGATGG TGCCCACCTT TGCCAGTCCA GACCTACTTA
    CATCAAGGAT CAGATGATGA ACTAGAAGAA GATGAAGAGA GAGTCCCTAC ACCCCCTGTA
    CGTGGGGTGG CATCATCACC TGCCATCTCA TTTGGACAAC AGTCAACAGC AACCCTCACA
    CCATCACCAC GTGAAGAAAT GCAACCCATG CTTCAGGCCC ACCTGGATGA ATTAACAAGA
    GCTTACCAGT TTGACATTGC AAAGCAATCT TGGCATTTAC ACAGCAATAA CCAGCCCCCA
    TTGGCCCCAG TTCCACCACT TGGATATGTG TCTGGGACAT TTATTTCTGA CTTAGAAAAA
    GACATTCCAG ATCAAGAAGA AGATGAAATT GAAGACGAAT CTATAGAAAT AACAAGGCCC
    CTCCGAGGTT TGGAACACAC TCCCGGTTCT AGTATGGATA ATCTCGACAG TTCTGTGACA
    GGCAAAATGA ACTCCTTTTC TCAGAGACCC CGGCCTGCCA GTCCCTTTTC TACTGACAGT
    AACACAAGTT CAGCTGTGAA TCATAGTCAG AAGTCACGGC CCACTAAGAA ACACAAGGGA
    GGCAGAATAG ATCAGCAACC CATGCTTCGC AGAGAAGGGA TTGCAGATGA TTTGCCTCCC
    CCACCTGATC CTCCACCTTG TCAAAGACAG CAAATCATGC CCAGTCAACG TGGTGGAGGT
    CCTACAGAAA GAAAAGCCAG TTCTCTTGAG AGGCACACAT CAAATATGAA TGATTTAAAA
    ACCTCATTAG ACCGGCATGG CAGAACATCC TTAGAACGGC AGCGACAAAC ACAGGAATGG
    CTTGGCTCGA CAGAAAGGCA GAACAAGCAG GCATTTGGAT CTGAAGAGAT ACTAATGCCA
    TACAGTAAAC CTAGCTTCCC ATCTCCAGGA GGGCACAGCT CCTCAGGAAC AGTCTCTTCA
    AAAGAATCAA CTGGACCCAG AAAAGGGGAG GTTTTGAGAG GTCACCAACG TAATGCCATT
    GACTTTCTTG ATGTAGGATA TTTGGGGCCA CACAGTCAAG GACAGTTTAC AGGTGAATTA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004912137.1
    CDS754..5256
    Translation

    Target ORF information:

    RefSeq Version XM_004912080.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004912080.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGCTTCC TGGTGGTGCC CCTGCTGTGT GGATTGTTAT GCTGTCAGGT GTGGGGATCC 
    CGTCTCCGTC AAGAAGATTT CCCCCCACGG ATAGTTGAGC ATCCATCGGA TGTCATTGTG
    TCAAAGGGAG AGCCAACCAC CTTGAACTGC AAAGCGGAAG GGCGGCCTAC ACCCACCATA
    GAGTGGTACA AAGACTTAGA AAGGGTTGAG ACAGACAAGG ATGACCCCCG CTCACATCGA
    ATGCTACTGC CAAGTGGATC TTTATTTTTT CTGCGCATTA TCCATGGGCG CAAGTTTAAG
    CCAGACGAAG GAAGTTACGT CTGTGTGGCA AGGAACTATC TCGGGGAGGC TATGAGTCGA
    AATGCATCTC TGGAAGTGGC ATTGCTCCGT GATGACTTCC GGCTCAACCC TACAGATGTT
    GTTGTGGCAG CTGGGGAACC TGCAATTTTG GAGTGTCAAC CCCCCAGAGG ACATCCGGAA
    CCAACTATAT ACTGGAAAAA GGACAAAGTT AGAATTGATG ATAAAGATGA AAGAATTAGT
    ATTCGAGGTG GAAAGCTGAT GCTCTCCAAT ACAAGGAAAA GTGATGCAGG CATGTACACT
    TGTGTTGGGA CCAACATGGT TGGAGAAAGA GACAGTGACC CAGCAGAGCT AACAGTCTTT
    GAACGTCCCA CATTCCTCAG AAGGCCAATT AATCAAGTTG TTTTAGAGGA TGAAGCTGTA
    GAATTTCGAT GTCAGGTCCA GGGGGACCCA CAGCCAACTG TCCGGTGGAA AAAGGATGAT
    GTAGATTTGC CAAGAGGAAG ATACGACATC AAGGATGACT ATACTTTGAG AATCAAGAAG
    GCTTCCAGCA GCGACGAAGG GACCTTTGCT TGTATTTCTG AAAACCGGGT TGGTAAAATA
    GAAGCTTCTG CCACACTTAC AGTCAGAGTT CGCCCTGTTG CTCCTCCCCA GTTTGTGATC
    AGACCAAGGG ATCAGATTGT AGCACAAGGC CGGACAGTAA CATTTGGATG TGAAGCTAAA
    GGAAATCCTC AACCAGCTGT TTTTTGGCAA AAAGAAGGAA GCCAGAATTT ACTTTTTCCA
    AATCAGCCTC TTCAGCCCAA CAGCAGGTAC TCAGTGTCAC CAACTGGAGA TTTAACCATC
    ACCAGCATTC AGCGCTCAGA CGCAGGCTAC TACATTTGTC AGGCACTGAC TGTTGCCGGA
    AGCATTCTGG CCAAGGCCCA GCTGGAAGTG ACTGATGTTT TGACAGATAG ACCACCGCCA
    ATAATTCTAC AAGGTCCAGC TAATCAAACT CTTGCCGTGG ATGGAACAGC AGTGCTGAAA
    TGTAAAACTA CTGGGGACCC AATGCCAGTC ATAAGTTGGC TAAAAGAGGG ATTTAAGGGA
    TTCATAGGGA GAGATGCAAG GTTATCCATT CTAGACCAAG GGACATTACA AATTAAAGGT
    TTAAAGGTTA CTGATTCTGG CACCTATACT TGTGTGGCTA CTAGCTCTAG TGGTGAGACA
    ACATGGAGCG CTGTTCTGGA AGTGACAGAA TCTGGTGGTG CCATAATTAG CAAGAACTAT
    GATCTCAATG ACCTTCCTGG ATCTCCATCA AAACCTCAGG TTACAGATGT CAGTAAGAAC
    AGTGTTACCC TATCATGGCA ACCAGGATCA CCAGGGATTC TGCCTGTGAC ATCCTACATC
    ATTGAGGCTT TCAGTCAATC TGTGAGCAAT AGTTGGCAAA CTGTGGCCAA TCACGTGAAG
    ACAACGCTTT ACACTGTTAA AGGACTGCGC CCAAACACCA TTTATCTCTT TATGGTGAGA
    GCAATCAATA CTGAAGGACT GAGTGACCCA AGTCCAATAT CTGATCCAGT GCGTACACAA
    GATATAAGTC CACCTGTTCA AGGAGTAGAT CACCGACAAG TACAGAAAGA ACTTGGGGAT
    GTGATTGTAC GGCTCCATAA TCCTGTTGTT CTGACACCAA CAACAATCCA GGTCACCTGG
    ATGGTCGACC GTCAATCCCA GTTTATTCAG GGATACAGGG TAATGTACCG TCAGACTTCT
    GGTCTGCAGA CTCCATCTGC GTGGCAGAGC TTAGAGGTCA AAGTGCCTGC AGAGCGTAGC
    GCAGTGCTAA CCAACCTGAA AAAAGGAATT ACTTATGAGA TTAAGGTTCG ACCTTATTTT
    AATGAATTTC AAGGAATGGA TAGCGAGTCA AAGTCAGCAC GAACAACTGA AGAAGCCCCA
    AGTGCTCCTC CTCAGTCTGT TACTGTTATG ACAGTGGGAA ATAACAACAG CACCAGCATC
    AGCATCTCAT GGGACCCCCC TCCATCTGAC CACCAGAATG GAATTATCCA GGACTACAGG
    ATCTGGTGCC TGGGTAATGA GACACGTTTC CACATCAACA AGACAGTGGA TGCTGCTATT
    CGTTCCGTGG TTATCGGTGG GCTATTCCCT GGAATTCAGT ACCGCGTTGA AGTCGCAGCC
    AGTACAAGCG CAGGCACTGG GGTAAAAAGT GCTCCCCAGC ACATACAGAT AGGAAATCGG
    AATGAAGTTG TGATCACAGA GAATAACAAC AGCATAACAG AGCAAATAAC TGATGTTGTG
    AAACAGCCGG CATTCATCGC AGGAATTGGT GGTGCCTGCT GGGTAATACT CATGGGATTT
    AGCATTTGGC TCTACTGGCG AAGAAAGAAG AGAAAGGGTC TCAGCAACTA CGCTGTTACA
    TTCCAGAGAG GAGATGGAGG ACTAATGAGT AATGGAAGTC GTCCTGGACT TTTAAATGCG
    AATGATCCTA GTTACCCTTG GTTGGCAGAC TCCTGGCCAG CTTCCAGCAT GCCGGTGAAT
    AACAGCAACA ATGGACCAAA CGATCTCGTT AATTTTAGTT GTGGAGATGT GCTGCCAACA
    GTTCCAGGTC AAGGAGAAAA AACAGCGACT ATGCTTTCTG ATGGAGCCAT TTATAGCAGC
    ATTGATTTTA CTACAAAATC TAGCTATAAT TGCCCAGGAC AAATTGCACA AACCACGCCA
    TATGCTACTA CGCAGATACT ACATTCCAAC AGCATTCATG AGCTGGCAGT TGACTTGCCT
    GATCCACAGT GGAAATCTTC CATTCAGCAG AAATCTGATC TCACTGGATT AGGCTATTGC
    CTTACTGACC AAGGCAAAGG GAGTAATGCC TTACTTTACA TTCCTGACTA TCGAATGGCT
    GAGGGATTGT CAAATAGAAT ACCCCACAAC CAGTCACAGG ATTTCAGCAC CACCAGCTCT
    CACAACAGCT CAGAGAGAAG TGGCAGCCTT TCAGGTGGAA AAGACGGAAA AAGGAAGAAA
    AATAAAAACA GCTCTAAAAT CTCTAAAAAC AATGGATCCA GTTGGGCCAA TGTTCCACTT
    CCGCCCCCTC CCATCCAACC ACTGCCAGGC ACTGAAATAG AACATTATGG CCTTGAACAC
    CAGGAAACAG GCTATGACAC CAATGGATGG TGCCCACCTT TGCCAGTCCA GACCTACTTA
    CATCAAGGAT CAGATGATGA ACTAGAAGAA GATGAAGAGA GAGTCCCTAC ACCCCCTGTA
    CGTGGGGTGG CATCATCACC TGCCATCTCA TTTGGACAAC AGTCAACAGC AACCCTCACA
    CCATCACCAC GTGAAGAAAT GCAACCCATG CTTCAGGCCC ACCTGGATGA ATTAACAAGA
    GCTTACCAGT TTGACATTGC AAAGCAATCT TGGCATTTAC ACAGCAATAA CCAGCCCCCA
    TTGGCCCCAG TTCCACCACT TGGATATGTG TCTGGGACAT TTATTTCTGA CTTAGAAAAA
    GACATTCCAG ATCAAGAAGA AGATGAAATT GAAGACGAAT CTATAGAAAT AACAAGGCCC
    CTCCGAGGTT TGGAACACAC TCCCGGTTCT AGTATGGATA ATCTCGACAG TTCTGTGACA
    GGCAAAATGA ACTCCTTTTC TCAGAGACCC CGGCCTGCCA GTCCCTTTTC TACTGACAGT
    AACACAAGTT CAGCTGTGAA TCATAGTCAG AAGTCACGGC CCACTAAGAA ACACAAGGGA
    GGCAGAATAG ATCAGCAACC CATGCTTCGC AGAGAAGGGA TTGCAGATGA TTTGCCTCCC
    CCACCTGATC CTCCACCTTG TCAAAGACAG CAAATCATGC CCAGTCAACG TGGTGGAGGT
    CCTACAGAAA GAAAAGCCAG TTCTCTTGAG AGGCACACAT CAAATATGAA TGATTTAAAA
    ACCTCATTAG ACCGGCATGG CAGAACATCC TTAGAACGGC AGCGACAAAC ACAGGAATGG
    CTTGGCTCGA CAGAAAGGCA GAACAAGCAG GCATTTGGAT CTGAAGAGAT ACTAATGCCA
    TACAGTAAAC CTAGCTTCCC ATCTCCAGGA GGGCACAGCT CCTCAGGAAC AGTCTCTTCA
    AAAGAATCAA CTGGACCCAG AAAAGGGGAG GTTTTGAGAG GTCACCAACG TAATGCCATT
    GACTTTCTTG ATGTAGGATA TTTGGGGCCA CACAGTCAAG GACAGTTTAC AGGTGAATTA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa06336
    Clone ID Related Accession (Same CDS sequence) XM_002931767.4
    Accession Version XM_002931767.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4491bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product roundabout homolog 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002931767.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGGCTTCC TGGTGGTGCC CCTGCTGTGT GGATTGTTAT GCTGTCAGGT GTGGGGATCC 
    CGTCTCCGTC AAGAAGATTT CCCCCCACGG ATAGTTGAGC ATCCATCGGA TGTCATTGTG
    TCAAAGGGAG AGCCAACCAC CTTGAACTGC AAAGCGGAAG GGCGGCCTAC ACCCACCATA
    GAGTGGTACA AAGACTTAGA AAGGGTTGAG ACAGACAAGG ATGACCCCCG CTCACATCGA
    ATGCTACTGC CAAGTGGATC TTTATTTTTT CTGCGCATTA TCCATGGGCG CAAGTTTAAG
    CCAGACGAAG GAAGTTACGT CTGTGTGGCA AGGAACTATC TCGGGGAGGC TATGAGTCGA
    AATGCATCTC TGGAAGTGGC ATTGCTCCGT GATGACTTCC GGCTCAACCC TACAGATGTT
    GTTGTGGCAG CTGGGGAACC TGCAATTTTG GAGTGTCAAC CCCCCAGAGG ACATCCGGAA
    CCAACTATAT ACTGGAAAAA GGACAAAGTT AGAATTGATG ATAAAGATGA AAGAATTAGT
    ATTCGAGGTG GAAAGCTGAT GCTCTCCAAT ACAAGGAAAA GTGATGCAGG CATGTACACT
    TGTGTTGGGA CCAACATGGT TGGAGAAAGA GACAGTGACC CAGCAGAGCT AACAGTCTTT
    GAACGTCCCA CATTCCTCAG AAGGCCAATT AATCAAGTTG TTTTAGAGGA TGAAGCTGTA
    GAATTTCGAT GTCAGGTCCA GGGGGACCCA CAGCCAACTG TCCGGTGGAA AAAGGATGAT
    GTAGATTTGC CAAGAGGAAG ATACGACATC AAGGATGACT ATACTTTGAG AATCAAGAAG
    GCTTCCAGCA GCGACGAAGG GACCTTTGCT TGTATTTCTG AAAACCGGGT TGGTAAAATA
    GAAGCTTCTG CCACACTTAC AGTCAGAGCT CCTCCCCAGT TTGTGATCAG ACCAAGGGAT
    CAGATTGTAG CACAAGGCCG GACAGTAACA TTTGGATGTG AAGCTAAAGG AAATCCTCAA
    CCAGCTGTTT TTTGGCAAAA AGAAGGAAGC CAGAATTTAC TTTTTCCAAA TCAGCCTCTT
    CAGCCCAACA GCAGGTACTC AGTGTCACCA ACTGGAGATT TAACCATCAC CAGCATTCAG
    CGCTCAGACG CAGGCTACTA CATTTGTCAG GCACTGACTG TTGCCGGAAG CATTCTGGCC
    AAGGCCCAGC TGGAAGTGAC TGATGTTTTG ACAGATAGAC CACCGCCAAT AATTCTACAA
    GGTCCAGCTA ATCAAACTCT TGCCGTGGAT GGAACAGCAG TGCTGAAATG TAAAACTACT
    GGGGACCCAA TGCCAGTCAT AAGTTGGCTA AAAGAGGGAT TTAAGGGATT CATAGGGAGA
    GATGCAAGGT TATCCATTCT AGACCAAGGG ACATTACAAA TTAAAGGTTT AAAGGTTACT
    GATTCTGGCA CCTATACTTG TGTGGCTACT AGCTCTAGTG GTGAGACAAC ATGGAGCGCT
    GTTCTGGAAG TGACAGAATC TGGTGGTGCC ATAATTAGCA AGAACTATGA TCTCAATGAC
    CTTCCTGGAT CTCCATCAAA ACCTCAGGTT ACAGATGTCA GTAAGAACAG TGTTACCCTA
    TCATGGCAAC CAGGATCACC AGGGATTCTG CCTGTGACAT CCTACATCAT TGAGGCTTTC
    AGTCAATCTG TGAGCAATAG TTGGCAAACT GTGGCCAATC ACGTGAAGAC AACGCTTTAC
    ACTGTTAAAG GACTGCGCCC AAACACCATT TATCTCTTTA TGGTGAGAGC AATCAATACT
    GAAGGACTGA GTGACCCAAG TCCAATATCT GATCCAGTGC GTACACAAGA TATAAGTCCA
    CCTGTTCAAG GAGTAGATCA CCGACAAGTA CAGAAAGAAC TTGGGGATGT GATTGTACGG
    CTCCATAATC CTGTTGTTCT GACACCAACA ACAATCCAGG TCACCTGGAT GGTCGACCGT
    CAATCCCAGT TTATTCAGGG ATACAGGGTA ATGTACCGTC AGACTTCTGG TCTGCAGACT
    CCATCTGCGT GGCAGAGCTT AGAGGTCAAA GTGCCTGCAG AGCGTAGCGC AGTGCTAACC
    AACCTGAAAA AAGGAATTAC TTATGAGATT AAGGTTCGAC CTTATTTTAA TGAATTTCAA
    GGAATGGATA GCGAGTCAAA GTCAGCACGA ACAACTGAAG AAGCCCCAAG TGCTCCTCCT
    CAGTCTGTTA CTGTTATGAC AGTGGGAAAT AACAACAGCA CCAGCATCAG CATCTCATGG
    GACCCCCCTC CATCTGACCA CCAGAATGGA ATTATCCAGG ACTACAGGAT CTGGTGCCTG
    GGTAATGAGA CACGTTTCCA CATCAACAAG ACAGTGGATG CTGCTATTCG TTCCGTGGTT
    ATCGGTGGGC TATTCCCTGG AATTCAGTAC CGCGTTGAAG TCGCAGCCAG TACAAGCGCA
    GGCACTGGGG TAAAAAGTGC TCCCCAGCAC ATACAGATAG GAAATCGGAA TGAAGTTGTG
    ATCACAGAGA ATAACAACAG CATAACAGAG CAAATAACTG ATGTTGTGAA ACAGCCGGCA
    TTCATCGCAG GAATTGGTGG TGCCTGCTGG GTAATACTCA TGGGATTTAG CATTTGGCTC
    TACTGGCGAA GAAAGAAGAG AAAGGGTCTC AGCAACTACG CTGTTACATT CCAGAGAGGA
    GATGGAGGAC TAATGAGTAA TGGAAGTCGT CCTGGACTTT TAAATGCGAA TGATCCTAGT
    TACCCTTGGT TGGCAGACTC CTGGCCAGCT TCCAGCATGC CGGTGAATAA CAGCAACAAT
    GGACCAAACG ATCTCGTTAA TTTTAGTTGT GGAGATGTGC TGCCAACAGT TCCAGGTCAA
    GGAGAAAAAA CAGCGACTAT GCTTTCTGAT GGAGCCATTT ATAGCAGCAT TGATTTTACT
    ACAAAATCTA GCTATAATTG CCCAGGACAA ATTGCACAAA CCACGCCATA TGCTACTACG
    CAGATACTAC ATTCCAACAG CATTCATGAG CTGGCAGTTG ACTTGCCTGA TCCACAGTGG
    AAATCTTCCA TTCAGCAGAA ATCTGATCTC ACTGGATTAG GCTATTGCCT TACTGACCAA
    GGCAAAGGGA GTAATGCCTT ACTTTACATT CCTGACTATC GAATGGCTGA GGGATTGTCA
    AATAGAATAC CCCACAACCA GTCACAGGAT TTCAGCACCA CCAGCTCTCA CAACAGCTCA
    GAGAGAAGTG GCAGCCTTTC AGGTGGAAAA GACGGAAAAA GGAAGAAAAA TAAAAACAGC
    TCTAAAATCT CTAAAAACAA TGGATCCAGT TGGGCCAATG TTCCACTTCC GCCCCCTCCC
    ATCCAACCAC TGCCAGGCAC TGAAATAGAA CATTATGGCC TTGAACACCA GGAAACAGGC
    TATGACACCA ATGGATGGTG CCCACCTTTG CCAGTCCAGA CCTACTTACA TCAAGGATCA
    GATGATGAAC TAGAAGAAGA TGAAGAGAGA GTCCCTACAC CCCCTGTACG TGGGGTGGCA
    TCATCACCTG CCATCTCATT TGGACAACAG TCAACAGCAA CCCTCACACC ATCACCACGT
    GAAGAAATGC AACCCATGCT TCAGGCCCAC CTGGATGAAT TAACAAGAGC TTACCAGTTT
    GACATTGCAA AGCAATCTTG GCATTTACAC AGCAATAACC AGCCCCCATT GGCCCCAGTT
    CCACCACTTG GATATGTGTC TGGGACATTT ATTTCTGACT TAGAAAAAGA CATTCCAGAT
    CAAGAAGAAG ATGAAATTGA AGACGAATCT ATAGAAATAA CAAGGCCCCT CCGAGGTTTG
    GAACACACTC CCGGTTCTAG TATGGATAAT CTCGACAGTT CTGTGACAGG CAAAATGAAC
    TCCTTTTCTC AGAGACCCCG GCCTGCCAGT CCCTTTTCTA CTGACAGTAA CACAAGTTCA
    GCTGTGAATC ATAGTCAGAA GTCACGGCCC ACTAAGAAAC ACAAGGGAGG CAGAATAGAT
    CAGCAACCCA TGCTTCGCAG AGAAGGGATT GCAGATGATT TGCCTCCCCC ACCTGATCCT
    CCACCTTGTC AAAGACAGCA AATCATGCCC AGTCAACGTG GTGGAGGTCC TACAGAAAGA
    AAAGCCAGTT CTCTTGAGAG GCACACATCA AATATGAATG ATTTAAAAAC CTCATTAGAC
    CGGCATGGCA GAACATCCTT AGAACGGCAG CGACAAACAC AGGAATGGCT TGGCTCGACA
    GAAAGGCAGA ACAAGCAGGC ATTTGGATCT GAAGAGATAC TAATGCCATA CAGTAAACCT
    AGCTTCCCAT CTCCAGGAGG GCACAGCTCC TCAGGAACAG TCTCTTCAAA AGAATCAACT
    GGACCCAGAA AAGGGGAGGT TTTGAGAGGT CACCAACGTA ATGCCATTGA CTTTCTTGAT
    GTAGGATATT TGGGGCCACA CAGTCAAGGA CAGTTTACAG GTGAATTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002931813.2
    CDS753..5243
    Translation

    Target ORF information:

    RefSeq Version XM_002931767.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002931767.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGGCTTCC TGGTGGTGCC CCTGCTGTGT GGATTGTTAT GCTGTCAGGT GTGGGGATCC 
    CGTCTCCGTC AAGAAGATTT CCCCCCACGG ATAGTTGAGC ATCCATCGGA TGTCATTGTG
    TCAAAGGGAG AGCCAACCAC CTTGAACTGC AAAGCGGAAG GGCGGCCTAC ACCCACCATA
    GAGTGGTACA AAGACTTAGA AAGGGTTGAG ACAGACAAGG ATGACCCCCG CTCACATCGA
    ATGCTACTGC CAAGTGGATC TTTATTTTTT CTGCGCATTA TCCATGGGCG CAAGTTTAAG
    CCAGACGAAG GAAGTTACGT CTGTGTGGCA AGGAACTATC TCGGGGAGGC TATGAGTCGA
    AATGCATCTC TGGAAGTGGC ATTGCTCCGT GATGACTTCC GGCTCAACCC TACAGATGTT
    GTTGTGGCAG CTGGGGAACC TGCAATTTTG GAGTGTCAAC CCCCCAGAGG ACATCCGGAA
    CCAACTATAT ACTGGAAAAA GGACAAAGTT AGAATTGATG ATAAAGATGA AAGAATTAGT
    ATTCGAGGTG GAAAGCTGAT GCTCTCCAAT ACAAGGAAAA GTGATGCAGG CATGTACACT
    TGTGTTGGGA CCAACATGGT TGGAGAAAGA GACAGTGACC CAGCAGAGCT AACAGTCTTT
    GAACGTCCCA CATTCCTCAG AAGGCCAATT AATCAAGTTG TTTTAGAGGA TGAAGCTGTA
    GAATTTCGAT GTCAGGTCCA GGGGGACCCA CAGCCAACTG TCCGGTGGAA AAAGGATGAT
    GTAGATTTGC CAAGAGGAAG ATACGACATC AAGGATGACT ATACTTTGAG AATCAAGAAG
    GCTTCCAGCA GCGACGAAGG GACCTTTGCT TGTATTTCTG AAAACCGGGT TGGTAAAATA
    GAAGCTTCTG CCACACTTAC AGTCAGAGCT CCTCCCCAGT TTGTGATCAG ACCAAGGGAT
    CAGATTGTAG CACAAGGCCG GACAGTAACA TTTGGATGTG AAGCTAAAGG AAATCCTCAA
    CCAGCTGTTT TTTGGCAAAA AGAAGGAAGC CAGAATTTAC TTTTTCCAAA TCAGCCTCTT
    CAGCCCAACA GCAGGTACTC AGTGTCACCA ACTGGAGATT TAACCATCAC CAGCATTCAG
    CGCTCAGACG CAGGCTACTA CATTTGTCAG GCACTGACTG TTGCCGGAAG CATTCTGGCC
    AAGGCCCAGC TGGAAGTGAC TGATGTTTTG ACAGATAGAC CACCGCCAAT AATTCTACAA
    GGTCCAGCTA ATCAAACTCT TGCCGTGGAT GGAACAGCAG TGCTGAAATG TAAAACTACT
    GGGGACCCAA TGCCAGTCAT AAGTTGGCTA AAAGAGGGAT TTAAGGGATT CATAGGGAGA
    GATGCAAGGT TATCCATTCT AGACCAAGGG ACATTACAAA TTAAAGGTTT AAAGGTTACT
    GATTCTGGCA CCTATACTTG TGTGGCTACT AGCTCTAGTG GTGAGACAAC ATGGAGCGCT
    GTTCTGGAAG TGACAGAATC TGGTGGTGCC ATAATTAGCA AGAACTATGA TCTCAATGAC
    CTTCCTGGAT CTCCATCAAA ACCTCAGGTT ACAGATGTCA GTAAGAACAG TGTTACCCTA
    TCATGGCAAC CAGGATCACC AGGGATTCTG CCTGTGACAT CCTACATCAT TGAGGCTTTC
    AGTCAATCTG TGAGCAATAG TTGGCAAACT GTGGCCAATC ACGTGAAGAC AACGCTTTAC
    ACTGTTAAAG GACTGCGCCC AAACACCATT TATCTCTTTA TGGTGAGAGC AATCAATACT
    GAAGGACTGA GTGACCCAAG TCCAATATCT GATCCAGTGC GTACACAAGA TATAAGTCCA
    CCTGTTCAAG GAGTAGATCA CCGACAAGTA CAGAAAGAAC TTGGGGATGT GATTGTACGG
    CTCCATAATC CTGTTGTTCT GACACCAACA ACAATCCAGG TCACCTGGAT GGTCGACCGT
    CAATCCCAGT TTATTCAGGG ATACAGGGTA ATGTACCGTC AGACTTCTGG TCTGCAGACT
    CCATCTGCGT GGCAGAGCTT AGAGGTCAAA GTGCCTGCAG AGCGTAGCGC AGTGCTAACC
    AACCTGAAAA AAGGAATTAC TTATGAGATT AAGGTTCGAC CTTATTTTAA TGAATTTCAA
    GGAATGGATA GCGAGTCAAA GTCAGCACGA ACAACTGAAG AAGCCCCAAG TGCTCCTCCT
    CAGTCTGTTA CTGTTATGAC AGTGGGAAAT AACAACAGCA CCAGCATCAG CATCTCATGG
    GACCCCCCTC CATCTGACCA CCAGAATGGA ATTATCCAGG ACTACAGGAT CTGGTGCCTG
    GGTAATGAGA CACGTTTCCA CATCAACAAG ACAGTGGATG CTGCTATTCG TTCCGTGGTT
    ATCGGTGGGC TATTCCCTGG AATTCAGTAC CGCGTTGAAG TCGCAGCCAG TACAAGCGCA
    GGCACTGGGG TAAAAAGTGC TCCCCAGCAC ATACAGATAG GAAATCGGAA TGAAGTTGTG
    ATCACAGAGA ATAACAACAG CATAACAGAG CAAATAACTG ATGTTGTGAA ACAGCCGGCA
    TTCATCGCAG GAATTGGTGG TGCCTGCTGG GTAATACTCA TGGGATTTAG CATTTGGCTC
    TACTGGCGAA GAAAGAAGAG AAAGGGTCTC AGCAACTACG CTGTTACATT CCAGAGAGGA
    GATGGAGGAC TAATGAGTAA TGGAAGTCGT CCTGGACTTT TAAATGCGAA TGATCCTAGT
    TACCCTTGGT TGGCAGACTC CTGGCCAGCT TCCAGCATGC CGGTGAATAA CAGCAACAAT
    GGACCAAACG ATCTCGTTAA TTTTAGTTGT GGAGATGTGC TGCCAACAGT TCCAGGTCAA
    GGAGAAAAAA CAGCGACTAT GCTTTCTGAT GGAGCCATTT ATAGCAGCAT TGATTTTACT
    ACAAAATCTA GCTATAATTG CCCAGGACAA ATTGCACAAA CCACGCCATA TGCTACTACG
    CAGATACTAC ATTCCAACAG CATTCATGAG CTGGCAGTTG ACTTGCCTGA TCCACAGTGG
    AAATCTTCCA TTCAGCAGAA ATCTGATCTC ACTGGATTAG GCTATTGCCT TACTGACCAA
    GGCAAAGGGA GTAATGCCTT ACTTTACATT CCTGACTATC GAATGGCTGA GGGATTGTCA
    AATAGAATAC CCCACAACCA GTCACAGGAT TTCAGCACCA CCAGCTCTCA CAACAGCTCA
    GAGAGAAGTG GCAGCCTTTC AGGTGGAAAA GACGGAAAAA GGAAGAAAAA TAAAAACAGC
    TCTAAAATCT CTAAAAACAA TGGATCCAGT TGGGCCAATG TTCCACTTCC GCCCCCTCCC
    ATCCAACCAC TGCCAGGCAC TGAAATAGAA CATTATGGCC TTGAACACCA GGAAACAGGC
    TATGACACCA ATGGATGGTG CCCACCTTTG CCAGTCCAGA CCTACTTACA TCAAGGATCA
    GATGATGAAC TAGAAGAAGA TGAAGAGAGA GTCCCTACAC CCCCTGTACG TGGGGTGGCA
    TCATCACCTG CCATCTCATT TGGACAACAG TCAACAGCAA CCCTCACACC ATCACCACGT
    GAAGAAATGC AACCCATGCT TCAGGCCCAC CTGGATGAAT TAACAAGAGC TTACCAGTTT
    GACATTGCAA AGCAATCTTG GCATTTACAC AGCAATAACC AGCCCCCATT GGCCCCAGTT
    CCACCACTTG GATATGTGTC TGGGACATTT ATTTCTGACT TAGAAAAAGA CATTCCAGAT
    CAAGAAGAAG ATGAAATTGA AGACGAATCT ATAGAAATAA CAAGGCCCCT CCGAGGTTTG
    GAACACACTC CCGGTTCTAG TATGGATAAT CTCGACAGTT CTGTGACAGG CAAAATGAAC
    TCCTTTTCTC AGAGACCCCG GCCTGCCAGT CCCTTTTCTA CTGACAGTAA CACAAGTTCA
    GCTGTGAATC ATAGTCAGAA GTCACGGCCC ACTAAGAAAC ACAAGGGAGG CAGAATAGAT
    CAGCAACCCA TGCTTCGCAG AGAAGGGATT GCAGATGATT TGCCTCCCCC ACCTGATCCT
    CCACCTTGTC AAAGACAGCA AATCATGCCC AGTCAACGTG GTGGAGGTCC TACAGAAAGA
    AAAGCCAGTT CTCTTGAGAG GCACACATCA AATATGAATG ATTTAAAAAC CTCATTAGAC
    CGGCATGGCA GAACATCCTT AGAACGGCAG CGACAAACAC AGGAATGGCT TGGCTCGACA
    GAAAGGCAGA ACAAGCAGGC ATTTGGATCT GAAGAGATAC TAATGCCATA CAGTAAACCT
    AGCTTCCCAT CTCCAGGAGG GCACAGCTCC TCAGGAACAG TCTCTTCAAA AGAATCAACT
    GGACCCAGAA AAGGGGAGGT TTTGAGAGGT CACCAACGTA ATGCCATTGA CTTTCTTGAT
    GTAGGATATT TGGGGCCACA CAGTCAAGGA CAGTTTACAG GTGAATTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35813
    Clone ID Related Accession (Same CDS sequence) XM_012957961.2
    Accession Version XM_012957961.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4596bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product roundabout homolog 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957961.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGCTTCC TGGTGGTGCC CCTGCTGTGT GGATTGTTAT GCTGTCAGGT GTGGGGATCC 
    CGTCTCCGTC AAGAAGATTT CCCCCCACGG ATAGTTGAGC ATCCATCGGA TGTCATTGTG
    TCAAAGGGAG AGCCAACCAC CTTGAACTGC AAAGCGGAAG GGCGGCCTAC ACCCACCATA
    GAGTGGTACA AAGACTTAGA AAGGGTTGAG ACAGACAAGG ATGACCCCCG CTCACATCGA
    ATGCTACTGC CAAGTGGATC TTTATTTTTT CTGCGCATTA TCCATGGGCG CAAGTTTAAG
    CCAGACGAAG GAAGTTACGT CTGTGTGGCA AGGAACTATC TCGGGGAGGC TATGAGTCGA
    AATGCATCTC TGGAAGTGGC ATTGCTCCGT GATGACTTCC GGCTCAACCC TACAGATGTT
    GTTGTGGCAG CTGGGGAACC TGCAATTTTG GAGTGTCAAC CCCCCAGAGG ACATCCGGAA
    CCAACTATAT ACTGGAAAAA GGACAAAGTT AGAATTGATG ATAAAGATGA AAGAATTAGT
    ATTCGAGGTG GAAAGCTGAT GCTCTCCAAT ACAAGGAAAA GTGATGCAGG CATGTACACT
    TGTGTTGGGA CCAACATGGT TGGAGAAAGA GACAGTGACC CAGCAGAGCT AACAGTCTTT
    GAACGTCCCA CATTCCTCAG AAGGCCAATT AATCAAGTTG TTTTAGAGGA TGAAGCTGTA
    GAATTTCGAT GTCAGGTCCA GGGGGACCCA CAGCCAACTG TCCGGTGGAA AAAGGATGAT
    GTAGATTTGC CAAGAGGAAG ATACGACATC AAGGATGACT ATACTTTGAG AATCAAGAAG
    GCTTCCAGCA GCGACGAAGG GACCTTTGCT TGTATTTCTG AAAACCGGGT TGGTAAAATA
    GAAGCTTCTG CCACACTTAC AGTCAGAGTT CGCCCTGTTG CTCCTCCCCA GTTTGTGATC
    AGACCAAGGG ATCAGATTGT AGCACAAGGC CGGACAGTAA CATTTGGATG TGAAGCTAAA
    GGAAATCCTC AACCAGCTGT TTTTTGGCAA AAAGAAGGAA GCCAGAATTT ACTTTTTCCA
    AATCAGCCTC TTCAGCCCAA CAGCAGGTAC TCAGTGTCAC CAACTGGAGA TTTAACCATC
    ACCAGCATTC AGCGCTCAGA CGCAGGCTAC TACATTTGTC AGGCACTGAC TGTTGCCGGA
    AGCATTCTGG CCAAGGCCCA GCTGGAAGTG ACTGATGTTT TGACAGATAG ACCACCGCCA
    ATAATTCTAC AAGGTCCAGC TAATCAAACT CTTGCCGTGG ATGGAACAGC AGTGCTGAAA
    TGTAAAACTA CTGGGGACCC AATGCCAGTC ATAAGTTGGC TAAAAGAGGG ATTTAAGGGA
    TTCATAGGGA GAGATGCAAG GTTATCCATT CTAGACCAAG GGACATTACA AATTAAAGGT
    TTAAAGGTTA CTGATTCTGG CACCTATACT TGTGTGGCTA CTAGCTCTAG TGGTGAGACA
    ACATGGAGCG CTGTTCTGGA AGTGACAGAA TCTGGTGGTG CCATAATTAG CAAGAACTAT
    GATCTCAATG ACCTTCCTGG ATCTCCATCA AAACCTCAGG TTACAGATGT CAGTAAGAAC
    AGTGTTACCC TATCATGGCA ACCAGGATCA CCAGGGATTC TGCCTGTGAC ATCCTACATC
    ATTGAGGCTT TCAGTCAATC TGTGAGCAAT AGTTGGCAAA CTGTGGCCAA TCACGTGAAG
    ACAACGCTTT ACACTGTTAA AGGACTGCGC CCAAACACCA TTTATCTCTT TATGGTGAGA
    GCAATCAATA CTGAAGGACT GAGTGACCCA AGTCCAATAT CTGATCCAGT GCGTACACAA
    GATATAAGTC CACCTGTTCA AGGAGTAGAT CACCGACAAG TACAGAAAGA ACTTGGGGAT
    GTGATTGTAC GGCTCCATAA TCCTGTTGTT CTGACACCAA CAACAATCCA GGTCACCTGG
    ATGGTCGACC GTCAATCCCA GTTTATTCAG GGATACAGGG TAATGTACCG TCAGACTTCT
    GGTCTGCAGA CTCCATCTGC GTGGCAGAGC TTAGAGGTCA AAGTGCCTGC AGAGCGTAGC
    GCAGTGCTAA CCAACCTGAA AAAAGGAATT ACTTATGAGA TTAAGGTTCG ACCTTATTTT
    AATGAATTTC AAGGAATGGA TAGCGAGTCA AAGTCAGCAC GAACAACTGA AGAAGCCCCA
    AGTGCTCCTC CTCAGTCTGT TACTGTTATG ACAGTGGGAA ATAACAACAG CACCAGCATC
    AGCATCTCAT GGGACCCCCC TCCATCTGAC CACCAGAATG GAATTATCCA GGACTACAGG
    ATCTGGTGCC TGGGTAATGA GACACGTTTC CACATCAACA AGACAGTGGA TGCTGCTATT
    CGTTCCGTGG TTATCGGTGG GCTATTCCCT GGAATTCAGT ACCGCGTTGA AGTCGCAGCC
    AGTACAAGCG CAGGCACTGG GGTAAAAAGT GCTCCCCAGC ACATACAGAT AGGAAATCGG
    AATGAAGTTG TGATCACAGA GAATAACAAC AGCATAACAG AGCAAATAAC TGATGTTGTG
    AAACAGCCGG CATTCATCGC AGGAATTGGT GGTGCCTGCT GGGTAATACT CATGGGATTT
    AGCATTTGGC TCTACTGGCG AAGAAAGAAG AGAAAGGGTC TCAGCAACTA CGCTGTTCAG
    TCTTTCACCT TTACCCCTGC AGATTCCCCA GATACAACCA TGCACCACAA GGTTGCACTT
    GGCACAAAGA AGGGGAGCTG GCACAAAACA GTTACATTCC AGAGAGGAGA TGGAGGACTA
    ATGAGTAATG GAAGTCGTCC TGGACTTTTA AATGCGAATG ATCCTAGTTA CCCTTGGTTG
    GCAGACTCCT GGCCAGCTTC CAGCATGCCG GTGAATAACA GCAACAATGG ACCAAACGAT
    CTCGTTAATT TTAGTTGTGG AGATGTGCTG CCAACAGTTC CAGGTCAAGG AGAAAAAACA
    GCGACTATGC TTTCTGATGG AGCCATTTAT AGCAGCATTG ATTTTACTAC AAAATCTAGC
    TATAATTGCC CAGGACAAAT TGCACAAACC ACGCCATATG CTACTACGCA GATACTACAT
    TCCAACAGCA TTCATGAGCT GGCAGTTGAC TTGCCTGATC CACAGTGGAA ATCTTCCATT
    CAGCAGAAAT CTGATCTCAC TGGATTAGGC TATTGCCTTA CTGACCAAGG CAAAGGGAGT
    AATGCCTTAC TTTACATTCC TGACTATCGA ATGGCTGAGG GATTGTCAAA TAGAATACCC
    CACAACCAGT CACAGGATTT CAGCACCACC AGCTCTCACA ACAGCTCAGA GAGAAGTGGC
    AGCCTTTCAG GTGGAAAAGA CGGAAAAAGG AAGAAAAATA AAAACAGCTC TAAAATCTCT
    AAAAACAATG GATCCAGTTG GGCCAATGTT CCACTTCCGC CCCCTCCCAT CCAACCACTG
    CCAGGCACTG AAATAGAACA TTATGGCCTT GAACACCAGG AAACAGGCTA TGACACCAAT
    GGATGGTGCC CACCTTTGCC AGTCCAGACC TACTTACATC AAGGATCAGA TGATGAACTA
    GAAGAAGATG AAGAGAGAGT CCCTACACCC CCTGTACGTG GGGTGGCATC ATCACCTGCC
    ATCTCATTTG GACAACAGTC AACAGCAACC CTCACACCAT CACCACGTGA AGAAATGCAA
    CCCATGCTTC AGGCCCACCT GGATGAATTA ACAAGAGCTT ACCAGTTTGA CATTGCAAAG
    CAATCTTGGC ATTTACACAG CAATAACCAG CCCCCATTGG CCCCAGTTCC ACCACTTGGA
    TATGTGTCTG GGACATTTAT TTCTGACTTA GAAAAAGACA TTCCAGATCA AGAAGAAGAT
    GAAATTGAAG ACGAATCTAT AGAAATAACA AGGCCCCTCC GAGGTTTGGA ACACACTCCC
    GGTTCTAGTA TGGATAATCT CGACAGTTCT GTGACAGGCA AAATGAACTC CTTTTCTCAG
    AGACCCCGGC CTGCCAGTCC CTTTTCTACT GACAGTAACA CAAGTTCAGC TGTGAATCAT
    AGTCAGAAGT CACGGCCCAC TAAGAAACAC AAGGGAGGCA GAATAGATCA GCAACCCATG
    CTTCGCAGAG AAGGGATTGC AGATGATTTG CCTCCCCCAC CTGATCCTCC ACCTTGTCAA
    AGACAGCAAA TCATGCCCAG TCAACGTGGT GGAGGTCCTA CAGAAAGAAA AGCCAGTTCT
    CTTGAGAGGC ACACATCAAA TATGAATGAT TTAAAAACCT CATTAGACCG GCATGGCAGA
    ACATCCTTAG AACGGCAGCG ACAAACACAG GAATGGCTTG GCTCGACAGA AAGGCAGAAC
    AAGCAGGCAT TTGGATCTGA AGAGATACTA ATGCCATACA GTAAACCTAG CTTCCCATCT
    CCAGGAGGGC ACAGCTCCTC AGGAACAGTC TCTTCAAAAG AATCAACTGG ACCCAGAAAA
    GGGGAGGTTT TGAGAGGTCA CCAACGTAAT GCCATTGACT TTCTTGATGT AGGATATTTG
    GGGCCACACA GTCAAGGACA GTTTACAGAG TACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813415.1
    CDS755..5350
    Translation

    Target ORF information:

    RefSeq Version XM_012957961.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957961.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGCTTCC TGGTGGTGCC CCTGCTGTGT GGATTGTTAT GCTGTCAGGT GTGGGGATCC 
    CGTCTCCGTC AAGAAGATTT CCCCCCACGG ATAGTTGAGC ATCCATCGGA TGTCATTGTG
    TCAAAGGGAG AGCCAACCAC CTTGAACTGC AAAGCGGAAG GGCGGCCTAC ACCCACCATA
    GAGTGGTACA AAGACTTAGA AAGGGTTGAG ACAGACAAGG ATGACCCCCG CTCACATCGA
    ATGCTACTGC CAAGTGGATC TTTATTTTTT CTGCGCATTA TCCATGGGCG CAAGTTTAAG
    CCAGACGAAG GAAGTTACGT CTGTGTGGCA AGGAACTATC TCGGGGAGGC TATGAGTCGA
    AATGCATCTC TGGAAGTGGC ATTGCTCCGT GATGACTTCC GGCTCAACCC TACAGATGTT
    GTTGTGGCAG CTGGGGAACC TGCAATTTTG GAGTGTCAAC CCCCCAGAGG ACATCCGGAA
    CCAACTATAT ACTGGAAAAA GGACAAAGTT AGAATTGATG ATAAAGATGA AAGAATTAGT
    ATTCGAGGTG GAAAGCTGAT GCTCTCCAAT ACAAGGAAAA GTGATGCAGG CATGTACACT
    TGTGTTGGGA CCAACATGGT TGGAGAAAGA GACAGTGACC CAGCAGAGCT AACAGTCTTT
    GAACGTCCCA CATTCCTCAG AAGGCCAATT AATCAAGTTG TTTTAGAGGA TGAAGCTGTA
    GAATTTCGAT GTCAGGTCCA GGGGGACCCA CAGCCAACTG TCCGGTGGAA AAAGGATGAT
    GTAGATTTGC CAAGAGGAAG ATACGACATC AAGGATGACT ATACTTTGAG AATCAAGAAG
    GCTTCCAGCA GCGACGAAGG GACCTTTGCT TGTATTTCTG AAAACCGGGT TGGTAAAATA
    GAAGCTTCTG CCACACTTAC AGTCAGAGTT CGCCCTGTTG CTCCTCCCCA GTTTGTGATC
    AGACCAAGGG ATCAGATTGT AGCACAAGGC CGGACAGTAA CATTTGGATG TGAAGCTAAA
    GGAAATCCTC AACCAGCTGT TTTTTGGCAA AAAGAAGGAA GCCAGAATTT ACTTTTTCCA
    AATCAGCCTC TTCAGCCCAA CAGCAGGTAC TCAGTGTCAC CAACTGGAGA TTTAACCATC
    ACCAGCATTC AGCGCTCAGA CGCAGGCTAC TACATTTGTC AGGCACTGAC TGTTGCCGGA
    AGCATTCTGG CCAAGGCCCA GCTGGAAGTG ACTGATGTTT TGACAGATAG ACCACCGCCA
    ATAATTCTAC AAGGTCCAGC TAATCAAACT CTTGCCGTGG ATGGAACAGC AGTGCTGAAA
    TGTAAAACTA CTGGGGACCC AATGCCAGTC ATAAGTTGGC TAAAAGAGGG ATTTAAGGGA
    TTCATAGGGA GAGATGCAAG GTTATCCATT CTAGACCAAG GGACATTACA AATTAAAGGT
    TTAAAGGTTA CTGATTCTGG CACCTATACT TGTGTGGCTA CTAGCTCTAG TGGTGAGACA
    ACATGGAGCG CTGTTCTGGA AGTGACAGAA TCTGGTGGTG CCATAATTAG CAAGAACTAT
    GATCTCAATG ACCTTCCTGG ATCTCCATCA AAACCTCAGG TTACAGATGT CAGTAAGAAC
    AGTGTTACCC TATCATGGCA ACCAGGATCA CCAGGGATTC TGCCTGTGAC ATCCTACATC
    ATTGAGGCTT TCAGTCAATC TGTGAGCAAT AGTTGGCAAA CTGTGGCCAA TCACGTGAAG
    ACAACGCTTT ACACTGTTAA AGGACTGCGC CCAAACACCA TTTATCTCTT TATGGTGAGA
    GCAATCAATA CTGAAGGACT GAGTGACCCA AGTCCAATAT CTGATCCAGT GCGTACACAA
    GATATAAGTC CACCTGTTCA AGGAGTAGAT CACCGACAAG TACAGAAAGA ACTTGGGGAT
    GTGATTGTAC GGCTCCATAA TCCTGTTGTT CTGACACCAA CAACAATCCA GGTCACCTGG
    ATGGTCGACC GTCAATCCCA GTTTATTCAG GGATACAGGG TAATGTACCG TCAGACTTCT
    GGTCTGCAGA CTCCATCTGC GTGGCAGAGC TTAGAGGTCA AAGTGCCTGC AGAGCGTAGC
    GCAGTGCTAA CCAACCTGAA AAAAGGAATT ACTTATGAGA TTAAGGTTCG ACCTTATTTT
    AATGAATTTC AAGGAATGGA TAGCGAGTCA AAGTCAGCAC GAACAACTGA AGAAGCCCCA
    AGTGCTCCTC CTCAGTCTGT TACTGTTATG ACAGTGGGAA ATAACAACAG CACCAGCATC
    AGCATCTCAT GGGACCCCCC TCCATCTGAC CACCAGAATG GAATTATCCA GGACTACAGG
    ATCTGGTGCC TGGGTAATGA GACACGTTTC CACATCAACA AGACAGTGGA TGCTGCTATT
    CGTTCCGTGG TTATCGGTGG GCTATTCCCT GGAATTCAGT ACCGCGTTGA AGTCGCAGCC
    AGTACAAGCG CAGGCACTGG GGTAAAAAGT GCTCCCCAGC ACATACAGAT AGGAAATCGG
    AATGAAGTTG TGATCACAGA GAATAACAAC AGCATAACAG AGCAAATAAC TGATGTTGTG
    AAACAGCCGG CATTCATCGC AGGAATTGGT GGTGCCTGCT GGGTAATACT CATGGGATTT
    AGCATTTGGC TCTACTGGCG AAGAAAGAAG AGAAAGGGTC TCAGCAACTA CGCTGTTCAG
    TCTTTCACCT TTACCCCTGC AGATTCCCCA GATACAACCA TGCACCACAA GGTTGCACTT
    GGCACAAAGA AGGGGAGCTG GCACAAAACA GTTACATTCC AGAGAGGAGA TGGAGGACTA
    ATGAGTAATG GAAGTCGTCC TGGACTTTTA AATGCGAATG ATCCTAGTTA CCCTTGGTTG
    GCAGACTCCT GGCCAGCTTC CAGCATGCCG GTGAATAACA GCAACAATGG ACCAAACGAT
    CTCGTTAATT TTAGTTGTGG AGATGTGCTG CCAACAGTTC CAGGTCAAGG AGAAAAAACA
    GCGACTATGC TTTCTGATGG AGCCATTTAT AGCAGCATTG ATTTTACTAC AAAATCTAGC
    TATAATTGCC CAGGACAAAT TGCACAAACC ACGCCATATG CTACTACGCA GATACTACAT
    TCCAACAGCA TTCATGAGCT GGCAGTTGAC TTGCCTGATC CACAGTGGAA ATCTTCCATT
    CAGCAGAAAT CTGATCTCAC TGGATTAGGC TATTGCCTTA CTGACCAAGG CAAAGGGAGT
    AATGCCTTAC TTTACATTCC TGACTATCGA ATGGCTGAGG GATTGTCAAA TAGAATACCC
    CACAACCAGT CACAGGATTT CAGCACCACC AGCTCTCACA ACAGCTCAGA GAGAAGTGGC
    AGCCTTTCAG GTGGAAAAGA CGGAAAAAGG AAGAAAAATA AAAACAGCTC TAAAATCTCT
    AAAAACAATG GATCCAGTTG GGCCAATGTT CCACTTCCGC CCCCTCCCAT CCAACCACTG
    CCAGGCACTG AAATAGAACA TTATGGCCTT GAACACCAGG AAACAGGCTA TGACACCAAT
    GGATGGTGCC CACCTTTGCC AGTCCAGACC TACTTACATC AAGGATCAGA TGATGAACTA
    GAAGAAGATG AAGAGAGAGT CCCTACACCC CCTGTACGTG GGGTGGCATC ATCACCTGCC
    ATCTCATTTG GACAACAGTC AACAGCAACC CTCACACCAT CACCACGTGA AGAAATGCAA
    CCCATGCTTC AGGCCCACCT GGATGAATTA ACAAGAGCTT ACCAGTTTGA CATTGCAAAG
    CAATCTTGGC ATTTACACAG CAATAACCAG CCCCCATTGG CCCCAGTTCC ACCACTTGGA
    TATGTGTCTG GGACATTTAT TTCTGACTTA GAAAAAGACA TTCCAGATCA AGAAGAAGAT
    GAAATTGAAG ACGAATCTAT AGAAATAACA AGGCCCCTCC GAGGTTTGGA ACACACTCCC
    GGTTCTAGTA TGGATAATCT CGACAGTTCT GTGACAGGCA AAATGAACTC CTTTTCTCAG
    AGACCCCGGC CTGCCAGTCC CTTTTCTACT GACAGTAACA CAAGTTCAGC TGTGAATCAT
    AGTCAGAAGT CACGGCCCAC TAAGAAACAC AAGGGAGGCA GAATAGATCA GCAACCCATG
    CTTCGCAGAG AAGGGATTGC AGATGATTTG CCTCCCCCAC CTGATCCTCC ACCTTGTCAA
    AGACAGCAAA TCATGCCCAG TCAACGTGGT GGAGGTCCTA CAGAAAGAAA AGCCAGTTCT
    CTTGAGAGGC ACACATCAAA TATGAATGAT TTAAAAACCT CATTAGACCG GCATGGCAGA
    ACATCCTTAG AACGGCAGCG ACAAACACAG GAATGGCTTG GCTCGACAGA AAGGCAGAAC
    AAGCAGGCAT TTGGATCTGA AGAGATACTA ATGCCATACA GTAAACCTAG CTTCCCATCT
    CCAGGAGGGC ACAGCTCCTC AGGAACAGTC TCTTCAAAAG AATCAACTGG ACCCAGAAAA
    GGGGAGGTTT TGAGAGGTCA CCAACGTAAT GCCATTGACT TTCTTGATGT AGGATATTTG
    GGGCCACACA GTCAAGGACA GTTTACAGAG TACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35814
    Clone ID Related Accession (Same CDS sequence) XM_012957962.2
    Accession Version XM_012957962.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4587bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product roundabout homolog 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957962.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGCTTCC TGGTGGTGCC CCTGCTGTGT GGATTGTTAT GCTGTCAGGT GTGGGGATCC 
    CGTCTCCGTC AAGAAGATTT CCCCCCACGG ATAGTTGAGC ATCCATCGGA TGTCATTGTG
    TCAAAGGGAG AGCCAACCAC CTTGAACTGC AAAGCGGAAG GGCGGCCTAC ACCCACCATA
    GAGTGGTACA AAGACTTAGA AAGGGTTGAG ACAGACAAGG ATGACCCCCG CTCACATCGA
    ATGCTACTGC CAAGTGGATC TTTATTTTTT CTGCGCATTA TCCATGGGCG CAAGTTTAAG
    CCAGACGAAG GAAGTTACGT CTGTGTGGCA AGGAACTATC TCGGGGAGGC TATGAGTCGA
    AATGCATCTC TGGAAGTGGC ATTGCTCCGT GATGACTTCC GGCTCAACCC TACAGATGTT
    GTTGTGGCAG CTGGGGAACC TGCAATTTTG GAGTGTCAAC CCCCCAGAGG ACATCCGGAA
    CCAACTATAT ACTGGAAAAA GGACAAAGTT AGAATTGATG ATAAAGATGA AAGAATTAGT
    ATTCGAGGTG GAAAGCTGAT GCTCTCCAAT ACAAGGAAAA GTGATGCAGG CATGTACACT
    TGTGTTGGGA CCAACATGGT TGGAGAAAGA GACAGTGACC CAGCAGAGCT AACAGTCTTT
    GAACGTCCCA CATTCCTCAG AAGGCCAATT AATCAAGTTG TTTTAGAGGA TGAAGCTGTA
    GAATTTCGAT GTCAGGTCCA GGGGGACCCA CAGCCAACTG TCCGGTGGAA AAAGGATGAT
    GTAGATTTGC CAAGAGGAAG ATACGACATC AAGGATGACT ATACTTTGAG AATCAAGAAG
    GCTTCCAGCA GCGACGAAGG GACCTTTGCT TGTATTTCTG AAAACCGGGT TGGTAAAATA
    GAAGCTTCTG CCACACTTAC AGTCAGAGCT CCTCCCCAGT TTGTGATCAG ACCAAGGGAT
    CAGATTGTAG CACAAGGCCG GACAGTAACA TTTGGATGTG AAGCTAAAGG AAATCCTCAA
    CCAGCTGTTT TTTGGCAAAA AGAAGGAAGC CAGAATTTAC TTTTTCCAAA TCAGCCTCTT
    CAGCCCAACA GCAGGTACTC AGTGTCACCA ACTGGAGATT TAACCATCAC CAGCATTCAG
    CGCTCAGACG CAGGCTACTA CATTTGTCAG GCACTGACTG TTGCCGGAAG CATTCTGGCC
    AAGGCCCAGC TGGAAGTGAC TGATGTTTTG ACAGATAGAC CACCGCCAAT AATTCTACAA
    GGTCCAGCTA ATCAAACTCT TGCCGTGGAT GGAACAGCAG TGCTGAAATG TAAAACTACT
    GGGGACCCAA TGCCAGTCAT AAGTTGGCTA AAAGAGGGAT TTAAGGGATT CATAGGGAGA
    GATGCAAGGT TATCCATTCT AGACCAAGGG ACATTACAAA TTAAAGGTTT AAAGGTTACT
    GATTCTGGCA CCTATACTTG TGTGGCTACT AGCTCTAGTG GTGAGACAAC ATGGAGCGCT
    GTTCTGGAAG TGACAGAATC TGGTGGTGCC ATAATTAGCA AGAACTATGA TCTCAATGAC
    CTTCCTGGAT CTCCATCAAA ACCTCAGGTT ACAGATGTCA GTAAGAACAG TGTTACCCTA
    TCATGGCAAC CAGGATCACC AGGGATTCTG CCTGTGACAT CCTACATCAT TGAGGCTTTC
    AGTCAATCTG TGAGCAATAG TTGGCAAACT GTGGCCAATC ACGTGAAGAC AACGCTTTAC
    ACTGTTAAAG GACTGCGCCC AAACACCATT TATCTCTTTA TGGTGAGAGC AATCAATACT
    GAAGGACTGA GTGACCCAAG TCCAATATCT GATCCAGTGC GTACACAAGA TATAAGTCCA
    CCTGTTCAAG GAGTAGATCA CCGACAAGTA CAGAAAGAAC TTGGGGATGT GATTGTACGG
    CTCCATAATC CTGTTGTTCT GACACCAACA ACAATCCAGG TCACCTGGAT GGTCGACCGT
    CAATCCCAGT TTATTCAGGG ATACAGGGTA ATGTACCGTC AGACTTCTGG TCTGCAGACT
    CCATCTGCGT GGCAGAGCTT AGAGGTCAAA GTGCCTGCAG AGCGTAGCGC AGTGCTAACC
    AACCTGAAAA AAGGAATTAC TTATGAGATT AAGGTTCGAC CTTATTTTAA TGAATTTCAA
    GGAATGGATA GCGAGTCAAA GTCAGCACGA ACAACTGAAG AAGCCCCAAG TGCTCCTCCT
    CAGTCTGTTA CTGTTATGAC AGTGGGAAAT AACAACAGCA CCAGCATCAG CATCTCATGG
    GACCCCCCTC CATCTGACCA CCAGAATGGA ATTATCCAGG ACTACAGGAT CTGGTGCCTG
    GGTAATGAGA CACGTTTCCA CATCAACAAG ACAGTGGATG CTGCTATTCG TTCCGTGGTT
    ATCGGTGGGC TATTCCCTGG AATTCAGTAC CGCGTTGAAG TCGCAGCCAG TACAAGCGCA
    GGCACTGGGG TAAAAAGTGC TCCCCAGCAC ATACAGATAG GAAATCGGAA TGAAGTTGTG
    ATCACAGAGA ATAACAACAG CATAACAGAG CAAATAACTG ATGTTGTGAA ACAGCCGGCA
    TTCATCGCAG GAATTGGTGG TGCCTGCTGG GTAATACTCA TGGGATTTAG CATTTGGCTC
    TACTGGCGAA GAAAGAAGAG AAAGGGTCTC AGCAACTACG CTGTTCAGTC TTTCACCTTT
    ACCCCTGCAG ATTCCCCAGA TACAACCATG CACCACAAGG TTGCACTTGG CACAAAGAAG
    GGGAGCTGGC ACAAAACAGT TACATTCCAG AGAGGAGATG GAGGACTAAT GAGTAATGGA
    AGTCGTCCTG GACTTTTAAA TGCGAATGAT CCTAGTTACC CTTGGTTGGC AGACTCCTGG
    CCAGCTTCCA GCATGCCGGT GAATAACAGC AACAATGGAC CAAACGATCT CGTTAATTTT
    AGTTGTGGAG ATGTGCTGCC AACAGTTCCA GGTCAAGGAG AAAAAACAGC GACTATGCTT
    TCTGATGGAG CCATTTATAG CAGCATTGAT TTTACTACAA AATCTAGCTA TAATTGCCCA
    GGACAAATTG CACAAACCAC GCCATATGCT ACTACGCAGA TACTACATTC CAACAGCATT
    CATGAGCTGG CAGTTGACTT GCCTGATCCA CAGTGGAAAT CTTCCATTCA GCAGAAATCT
    GATCTCACTG GATTAGGCTA TTGCCTTACT GACCAAGGCA AAGGGAGTAA TGCCTTACTT
    TACATTCCTG ACTATCGAAT GGCTGAGGGA TTGTCAAATA GAATACCCCA CAACCAGTCA
    CAGGATTTCA GCACCACCAG CTCTCACAAC AGCTCAGAGA GAAGTGGCAG CCTTTCAGGT
    GGAAAAGACG GAAAAAGGAA GAAAAATAAA AACAGCTCTA AAATCTCTAA AAACAATGGA
    TCCAGTTGGG CCAATGTTCC ACTTCCGCCC CCTCCCATCC AACCACTGCC AGGCACTGAA
    ATAGAACATT ATGGCCTTGA ACACCAGGAA ACAGGCTATG ACACCAATGG ATGGTGCCCA
    CCTTTGCCAG TCCAGACCTA CTTACATCAA GGATCAGATG ATGAACTAGA AGAAGATGAA
    GAGAGAGTCC CTACACCCCC TGTACGTGGG GTGGCATCAT CACCTGCCAT CTCATTTGGA
    CAACAGTCAA CAGCAACCCT CACACCATCA CCACGTGAAG AAATGCAACC CATGCTTCAG
    GCCCACCTGG ATGAATTAAC AAGAGCTTAC CAGTTTGACA TTGCAAAGCA ATCTTGGCAT
    TTACACAGCA ATAACCAGCC CCCATTGGCC CCAGTTCCAC CACTTGGATA TGTGTCTGGG
    ACATTTATTT CTGACTTAGA AAAAGACATT CCAGATCAAG AAGAAGATGA AATTGAAGAC
    GAATCTATAG AAATAACAAG GCCCCTCCGA GGTTTGGAAC ACACTCCCGG TTCTAGTATG
    GATAATCTCG ACAGTTCTGT GACAGGCAAA ATGAACTCCT TTTCTCAGAG ACCCCGGCCT
    GCCAGTCCCT TTTCTACTGA CAGTAACACA AGTTCAGCTG TGAATCATAG TCAGAAGTCA
    CGGCCCACTA AGAAACACAA GGGAGGCAGA ATAGATCAGC AACCCATGCT TCGCAGAGAA
    GGGATTGCAG ATGATTTGCC TCCCCCACCT GATCCTCCAC CTTGTCAAAG ACAGCAAATC
    ATGCCCAGTC AACGTGGTGG AGGTCCTACA GAAAGAAAAG CCAGTTCTCT TGAGAGGCAC
    ACATCAAATA TGAATGATTT AAAAACCTCA TTAGACCGGC ATGGCAGAAC ATCCTTAGAA
    CGGCAGCGAC AAACACAGGA ATGGCTTGGC TCGACAGAAA GGCAGAACAA GCAGGCATTT
    GGATCTGAAG AGATACTAAT GCCATACAGT AAACCTAGCT TCCCATCTCC AGGAGGGCAC
    AGCTCCTCAG GAACAGTCTC TTCAAAAGAA TCAACTGGAC CCAGAAAAGG GGAGGTTTTG
    AGAGGTCACC AACGTAATGC CATTGACTTT CTTGATGTAG GATATTTGGG GCCACACAGT
    CAAGGACAGT TTACAGGTGA ATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813416.1
    CDS755..5341
    Translation

    Target ORF information:

    RefSeq Version XM_012957962.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957962.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGCTTCC TGGTGGTGCC CCTGCTGTGT GGATTGTTAT GCTGTCAGGT GTGGGGATCC 
    CGTCTCCGTC AAGAAGATTT CCCCCCACGG ATAGTTGAGC ATCCATCGGA TGTCATTGTG
    TCAAAGGGAG AGCCAACCAC CTTGAACTGC AAAGCGGAAG GGCGGCCTAC ACCCACCATA
    GAGTGGTACA AAGACTTAGA AAGGGTTGAG ACAGACAAGG ATGACCCCCG CTCACATCGA
    ATGCTACTGC CAAGTGGATC TTTATTTTTT CTGCGCATTA TCCATGGGCG CAAGTTTAAG
    CCAGACGAAG GAAGTTACGT CTGTGTGGCA AGGAACTATC TCGGGGAGGC TATGAGTCGA
    AATGCATCTC TGGAAGTGGC ATTGCTCCGT GATGACTTCC GGCTCAACCC TACAGATGTT
    GTTGTGGCAG CTGGGGAACC TGCAATTTTG GAGTGTCAAC CCCCCAGAGG ACATCCGGAA
    CCAACTATAT ACTGGAAAAA GGACAAAGTT AGAATTGATG ATAAAGATGA AAGAATTAGT
    ATTCGAGGTG GAAAGCTGAT GCTCTCCAAT ACAAGGAAAA GTGATGCAGG CATGTACACT
    TGTGTTGGGA CCAACATGGT TGGAGAAAGA GACAGTGACC CAGCAGAGCT AACAGTCTTT
    GAACGTCCCA CATTCCTCAG AAGGCCAATT AATCAAGTTG TTTTAGAGGA TGAAGCTGTA
    GAATTTCGAT GTCAGGTCCA GGGGGACCCA CAGCCAACTG TCCGGTGGAA AAAGGATGAT
    GTAGATTTGC CAAGAGGAAG ATACGACATC AAGGATGACT ATACTTTGAG AATCAAGAAG
    GCTTCCAGCA GCGACGAAGG GACCTTTGCT TGTATTTCTG AAAACCGGGT TGGTAAAATA
    GAAGCTTCTG CCACACTTAC AGTCAGAGCT CCTCCCCAGT TTGTGATCAG ACCAAGGGAT
    CAGATTGTAG CACAAGGCCG GACAGTAACA TTTGGATGTG AAGCTAAAGG AAATCCTCAA
    CCAGCTGTTT TTTGGCAAAA AGAAGGAAGC CAGAATTTAC TTTTTCCAAA TCAGCCTCTT
    CAGCCCAACA GCAGGTACTC AGTGTCACCA ACTGGAGATT TAACCATCAC CAGCATTCAG
    CGCTCAGACG CAGGCTACTA CATTTGTCAG GCACTGACTG TTGCCGGAAG CATTCTGGCC
    AAGGCCCAGC TGGAAGTGAC TGATGTTTTG ACAGATAGAC CACCGCCAAT AATTCTACAA
    GGTCCAGCTA ATCAAACTCT TGCCGTGGAT GGAACAGCAG TGCTGAAATG TAAAACTACT
    GGGGACCCAA TGCCAGTCAT AAGTTGGCTA AAAGAGGGAT TTAAGGGATT CATAGGGAGA
    GATGCAAGGT TATCCATTCT AGACCAAGGG ACATTACAAA TTAAAGGTTT AAAGGTTACT
    GATTCTGGCA CCTATACTTG TGTGGCTACT AGCTCTAGTG GTGAGACAAC ATGGAGCGCT
    GTTCTGGAAG TGACAGAATC TGGTGGTGCC ATAATTAGCA AGAACTATGA TCTCAATGAC
    CTTCCTGGAT CTCCATCAAA ACCTCAGGTT ACAGATGTCA GTAAGAACAG TGTTACCCTA
    TCATGGCAAC CAGGATCACC AGGGATTCTG CCTGTGACAT CCTACATCAT TGAGGCTTTC
    AGTCAATCTG TGAGCAATAG TTGGCAAACT GTGGCCAATC ACGTGAAGAC AACGCTTTAC
    ACTGTTAAAG GACTGCGCCC AAACACCATT TATCTCTTTA TGGTGAGAGC AATCAATACT
    GAAGGACTGA GTGACCCAAG TCCAATATCT GATCCAGTGC GTACACAAGA TATAAGTCCA
    CCTGTTCAAG GAGTAGATCA CCGACAAGTA CAGAAAGAAC TTGGGGATGT GATTGTACGG
    CTCCATAATC CTGTTGTTCT GACACCAACA ACAATCCAGG TCACCTGGAT GGTCGACCGT
    CAATCCCAGT TTATTCAGGG ATACAGGGTA ATGTACCGTC AGACTTCTGG TCTGCAGACT
    CCATCTGCGT GGCAGAGCTT AGAGGTCAAA GTGCCTGCAG AGCGTAGCGC AGTGCTAACC
    AACCTGAAAA AAGGAATTAC TTATGAGATT AAGGTTCGAC CTTATTTTAA TGAATTTCAA
    GGAATGGATA GCGAGTCAAA GTCAGCACGA ACAACTGAAG AAGCCCCAAG TGCTCCTCCT
    CAGTCTGTTA CTGTTATGAC AGTGGGAAAT AACAACAGCA CCAGCATCAG CATCTCATGG
    GACCCCCCTC CATCTGACCA CCAGAATGGA ATTATCCAGG ACTACAGGAT CTGGTGCCTG
    GGTAATGAGA CACGTTTCCA CATCAACAAG ACAGTGGATG CTGCTATTCG TTCCGTGGTT
    ATCGGTGGGC TATTCCCTGG AATTCAGTAC CGCGTTGAAG TCGCAGCCAG TACAAGCGCA
    GGCACTGGGG TAAAAAGTGC TCCCCAGCAC ATACAGATAG GAAATCGGAA TGAAGTTGTG
    ATCACAGAGA ATAACAACAG CATAACAGAG CAAATAACTG ATGTTGTGAA ACAGCCGGCA
    TTCATCGCAG GAATTGGTGG TGCCTGCTGG GTAATACTCA TGGGATTTAG CATTTGGCTC
    TACTGGCGAA GAAAGAAGAG AAAGGGTCTC AGCAACTACG CTGTTCAGTC TTTCACCTTT
    ACCCCTGCAG ATTCCCCAGA TACAACCATG CACCACAAGG TTGCACTTGG CACAAAGAAG
    GGGAGCTGGC ACAAAACAGT TACATTCCAG AGAGGAGATG GAGGACTAAT GAGTAATGGA
    AGTCGTCCTG GACTTTTAAA TGCGAATGAT CCTAGTTACC CTTGGTTGGC AGACTCCTGG
    CCAGCTTCCA GCATGCCGGT GAATAACAGC AACAATGGAC CAAACGATCT CGTTAATTTT
    AGTTGTGGAG ATGTGCTGCC AACAGTTCCA GGTCAAGGAG AAAAAACAGC GACTATGCTT
    TCTGATGGAG CCATTTATAG CAGCATTGAT TTTACTACAA AATCTAGCTA TAATTGCCCA
    GGACAAATTG CACAAACCAC GCCATATGCT ACTACGCAGA TACTACATTC CAACAGCATT
    CATGAGCTGG CAGTTGACTT GCCTGATCCA CAGTGGAAAT CTTCCATTCA GCAGAAATCT
    GATCTCACTG GATTAGGCTA TTGCCTTACT GACCAAGGCA AAGGGAGTAA TGCCTTACTT
    TACATTCCTG ACTATCGAAT GGCTGAGGGA TTGTCAAATA GAATACCCCA CAACCAGTCA
    CAGGATTTCA GCACCACCAG CTCTCACAAC AGCTCAGAGA GAAGTGGCAG CCTTTCAGGT
    GGAAAAGACG GAAAAAGGAA GAAAAATAAA AACAGCTCTA AAATCTCTAA AAACAATGGA
    TCCAGTTGGG CCAATGTTCC ACTTCCGCCC CCTCCCATCC AACCACTGCC AGGCACTGAA
    ATAGAACATT ATGGCCTTGA ACACCAGGAA ACAGGCTATG ACACCAATGG ATGGTGCCCA
    CCTTTGCCAG TCCAGACCTA CTTACATCAA GGATCAGATG ATGAACTAGA AGAAGATGAA
    GAGAGAGTCC CTACACCCCC TGTACGTGGG GTGGCATCAT CACCTGCCAT CTCATTTGGA
    CAACAGTCAA CAGCAACCCT CACACCATCA CCACGTGAAG AAATGCAACC CATGCTTCAG
    GCCCACCTGG ATGAATTAAC AAGAGCTTAC CAGTTTGACA TTGCAAAGCA ATCTTGGCAT
    TTACACAGCA ATAACCAGCC CCCATTGGCC CCAGTTCCAC CACTTGGATA TGTGTCTGGG
    ACATTTATTT CTGACTTAGA AAAAGACATT CCAGATCAAG AAGAAGATGA AATTGAAGAC
    GAATCTATAG AAATAACAAG GCCCCTCCGA GGTTTGGAAC ACACTCCCGG TTCTAGTATG
    GATAATCTCG ACAGTTCTGT GACAGGCAAA ATGAACTCCT TTTCTCAGAG ACCCCGGCCT
    GCCAGTCCCT TTTCTACTGA CAGTAACACA AGTTCAGCTG TGAATCATAG TCAGAAGTCA
    CGGCCCACTA AGAAACACAA GGGAGGCAGA ATAGATCAGC AACCCATGCT TCGCAGAGAA
    GGGATTGCAG ATGATTTGCC TCCCCCACCT GATCCTCCAC CTTGTCAAAG ACAGCAAATC
    ATGCCCAGTC AACGTGGTGG AGGTCCTACA GAAAGAAAAG CCAGTTCTCT TGAGAGGCAC
    ACATCAAATA TGAATGATTT AAAAACCTCA TTAGACCGGC ATGGCAGAAC ATCCTTAGAA
    CGGCAGCGAC AAACACAGGA ATGGCTTGGC TCGACAGAAA GGCAGAACAA GCAGGCATTT
    GGATCTGAAG AGATACTAAT GCCATACAGT AAACCTAGCT TCCCATCTCC AGGAGGGCAC
    AGCTCCTCAG GAACAGTCTC TTCAAAAGAA TCAACTGGAC CCAGAAAAGG GGAGGTTTTG
    AGAGGTCACC AACGTAATGC CATTGACTTT CTTGATGTAG GATATTTGGG GCCACACAGT
    CAAGGACAGT TTACAGGTGA ATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35815
    Clone ID Related Accession (Same CDS sequence) XM_012957963.2
    Accession Version XM_012957963.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4530bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product roundabout homolog 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957963.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGCTTCC TGGTGGTGCC CCTGCTGTGT GGATTGTTAT GCTGTCAGGT GTGGGGATCC 
    CGTCTCCGTC AAGAAGATTT CCCCCCACGG ATAGTTGAGC ATCCATCGGA TGTCATTGTG
    TCAAAGGGAG AGCCAACCAC CTTGAACTGC AAAGCGGAAG GGCGGCCTAC ACCCACCATA
    GAGTGGTACA AAGACTTAGA AAGGGTTGAG ACAGACAAGG ATGACCCCCG CTCACATCGA
    ATGCTACTGC CAAGTGGATC TTTATTTTTT CTGCGCATTA TCCATGGGCG CAAGTTTAAG
    CCAGACGAAG GAAGTTACGT CTGTGTGGCA AGGAACTATC TCGGGGAGGC TATGAGTCGA
    AATGCATCTC TGGAAGTGGC ATTGCTCCGT GATGACTTCC GGCTCAACCC TACAGATGTT
    GTTGTGGCAG CTGGGGAACC TGCAATTTTG GAGTGTCAAC CCCCCAGAGG ACATCCGGAA
    CCAACTATAT ACTGGAAAAA GGACAAAGTT AGAATTGATG ATAAAGATGA AAGAATTAGT
    ATTCGAGGTG GAAAGCTGAT GCTCTCCAAT ACAAGGAAAA GTGATGCAGG CATGTACACT
    TGTGTTGGGA CCAACATGGT TGGAGAAAGA GACAGTGACC CAGCAGAGCT AACAGTCTTT
    GAACGTCCCA CATTCCTCAG AAGGCCAATT AATCAAGTTG TTTTAGAGGA TGAAGCTGTA
    GAATTTCGAT GTCAGGTCCA GGGGGACCCA CAGCCAACTG TCCGGTGGAA AAAGGATGAT
    GTAGATTTGC CAAGAGGAAG ATACGACATC AAGGATGACT ATACTTTGAG AATCAAGAAG
    GCTTCCAGCA GCGACGAAGG GACCTTTGCT TGTATTTCTG AAAACCGGGT TGGTAAAATA
    GAAGCTTCTG CCACACTTAC AGTCAGAGTT CGCCCTGTTG CTCCTCCCCA GTTTGTGATC
    AGACCAAGGG ATCAGATTGT AGCACAAGGC CGGACAGTAA CATTTGGATG TGAAGCTAAA
    GGAAATCCTC AACCAGCTGT TTTTTGGCAA AAAGAAGGAA GCCAGAATTT ACTTTTTCCA
    AATCAGCCTC TTCAGCCCAA CAGCAGGTAC TCAGTGTCAC CAACTGGAGA TTTAACCATC
    ACCAGCATTC AGCGCTCAGA CGCAGGCTAC TACATTTGTC AGGCACTGAC TGTTGCCGGA
    AGCATTCTGG CCAAGGCCCA GCTGGAAGTG ACTGATGTTT TGACAGATAG ACCACCGCCA
    ATAATTCTAC AAGGTCCAGC TAATCAAACT CTTGCCGTGG ATGGAACAGC AGTGCTGAAA
    TGTAAAACTA CTGGGGACCC AATGCCAGTC ATAAGTTGGC TAAAAGAGGG ATTTAAGGGA
    TTCATAGGGA GAGATGCAAG GTTATCCATT CTAGACCAAG GGACATTACA AATTAAAGGT
    TTAAAGGTTA CTGATTCTGG CACCTATACT TGTGTGGCTA CTAGCTCTAG TGGTGAGACA
    ACATGGAGCG CTGTTCTGGA AGTGACAGAA TCTGGTGGTG CCATAATTAG CAAGAACTAT
    GATCTCAATG ACCTTCCTGG ATCTCCATCA AAACCTCAGG TTACAGATGT CAGTAAGAAC
    AGTGTTACCC TATCATGGCA ACCAGGATCA CCAGGGATTC TGCCTGTGAC ATCCTACATC
    ATTGAGGCTT TCAGTCAATC TGTGAGCAAT AGTTGGCAAA CTGTGGCCAA TCACGTGAAG
    ACAACGCTTT ACACTGTTAA AGGACTGCGC CCAAACACCA TTTATCTCTT TATGGTGAGA
    GCAATCAATA CTGAAGGACT GAGTGACCCA AGTCCAATAT CTGATCCAGT GCGTACACAA
    GATATAAGTC CACCTGTTCA AGGAGTAGAT CACCGACAAG TACAGAAAGA ACTTGGGGAT
    GTGATTGTAC GGCTCCATAA TCCTGTTGTT CTGACACCAA CAACAATCCA GGTCACCTGG
    ATGGTCGACC GTCAATCCCA GTTTATTCAG GGATACAGGG TAATGTACCG TCAGACTTCT
    GGTCTGCAGA CTCCATCTGC GTGGCAGAGC TTAGAGGTCA AAGTGCCTGC AGAGCGTAGC
    GCAGTGCTAA CCAACCTGAA AAAAGGAATT ACTTATGAGA TTAAGGTTCG ACCTTATTTT
    AATGAATTTC AAGGAATGGA TAGCGAGTCA AAGTCAGCAC GAACAACTGA AGAAGCCCCA
    AGTGCTCCTC CTCAGTCTGT TACTGTTATG ACAGTGGGAA ATAACAACAG CACCAGCATC
    AGCATCTCAT GGGACCCCCC TCCATCTGAC CACCAGAATG GAATTATCCA GGACTACAGG
    ATCTGGTGCC TGGGTAATGA GACACGTTTC CACATCAACA AGACAGTGGA TGCTGCTATT
    CGTTCCGTGG TTATCGGTGG GCTATTCCCT GGAATTCAGT ACCGCGTTGA AGTCGCAGCC
    AGTACAAGCG CAGGCACTGG GGTAAAAAGT GCTCCCCAGC ACATACAGAT AGGAAATCGG
    AATGAAGTTG TGATCACAGA GAATAACAAC AGCATAACAG AGCAAATAAC TGATGTTGTG
    AAACAGCCGG CATTCATCGC AGGAATTGGT GGTGCCTGCT GGGTAATACT CATGGGATTT
    AGCATTTGGC TCTACTGGCG AAGAAAGAAG AGAAAGGGTC TCAGCAACTA CGCTGTTCAG
    TCTTTCACCT TTACCCCTGC AGTTACATTC CAGAGAGGAG ATGGAGGACT AATGAGTAAT
    GGAAGTCGTC CTGGACTTTT AAATGCGAAT GATCCTAGTT ACCCTTGGTT GGCAGACTCC
    TGGCCAGCTT CCAGCATGCC GGTGAATAAC AGCAACAATG GACCAAACGA TCTCGTTAAT
    TTTAGTTGTG GAGATGTGCT GCCAACAGTT CCAGGTCAAG GAGAAAAAAC AGCGACTATG
    CTTTCTGATG GAGCCATTTA TAGCAGCATT GATTTTACTA CAAAATCTAG CTATAATTGC
    CCAGGACAAA TTGCACAAAC CACGCCATAT GCTACTACGC AGATACTACA TTCCAACAGC
    ATTCATGAGC TGGCAGTTGA CTTGCCTGAT CCACAGTGGA AATCTTCCAT TCAGCAGAAA
    TCTGATCTCA CTGGATTAGG CTATTGCCTT ACTGACCAAG GCAAAGGGAG TAATGCCTTA
    CTTTACATTC CTGACTATCG AATGGCTGAG GGATTGTCAA ATAGAATACC CCACAACCAG
    TCACAGGATT TCAGCACCAC CAGCTCTCAC AACAGCTCAG AGAGAAGTGG CAGCCTTTCA
    GGTGGAAAAG ACGGAAAAAG GAAGAAAAAT AAAAACAGCT CTAAAATCTC TAAAAACAAT
    GGATCCAGTT GGGCCAATGT TCCACTTCCG CCCCCTCCCA TCCAACCACT GCCAGGCACT
    GAAATAGAAC ATTATGGCCT TGAACACCAG GAAACAGGCT ATGACACCAA TGGATGGTGC
    CCACCTTTGC CAGTCCAGAC CTACTTACAT CAAGGATCAG ATGATGAACT AGAAGAAGAT
    GAAGAGAGAG TCCCTACACC CCCTGTACGT GGGGTGGCAT CATCACCTGC CATCTCATTT
    GGACAACAGT CAACAGCAAC CCTCACACCA TCACCACGTG AAGAAATGCA ACCCATGCTT
    CAGGCCCACC TGGATGAATT AACAAGAGCT TACCAGTTTG ACATTGCAAA GCAATCTTGG
    CATTTACACA GCAATAACCA GCCCCCATTG GCCCCAGTTC CACCACTTGG ATATGTGTCT
    GGGACATTTA TTTCTGACTT AGAAAAAGAC ATTCCAGATC AAGAAGAAGA TGAAATTGAA
    GACGAATCTA TAGAAATAAC AAGGCCCCTC CGAGGTTTGG AACACACTCC CGGTTCTAGT
    ATGGATAATC TCGACAGTTC TGTGACAGGC AAAATGAACT CCTTTTCTCA GAGACCCCGG
    CCTGCCAGTC CCTTTTCTAC TGACAGTAAC ACAAGTTCAG CTGTGAATCA TAGTCAGAAG
    TCACGGCCCA CTAAGAAACA CAAGGGAGGC AGAATAGATC AGCAACCCAT GCTTCGCAGA
    GAAGGGATTG CAGATGATTT GCCTCCCCCA CCTGATCCTC CACCTTGTCA AAGACAGCAA
    ATCATGCCCA GTCAACGTGG TGGAGGTCCT ACAGAAAGAA AAGCCAGTTC TCTTGAGAGG
    CACACATCAA ATATGAATGA TTTAAAAACC TCATTAGACC GGCATGGCAG AACATCCTTA
    GAACGGCAGC GACAAACACA GGAATGGCTT GGCTCGACAG AAAGGCAGAA CAAGCAGGCA
    TTTGGATCTG AAGAGATACT AATGCCATAC AGTAAACCTA GCTTCCCATC TCCAGGAGGG
    CACAGCTCCT CAGGAACAGT CTCTTCAAAA GAATCAACTG GACCCAGAAA AGGGGAGGTT
    TTGAGAGGTC ACCAACGTAA TGCCATTGAC TTTCTTGATG TAGGATATTT GGGGCCACAC
    AGTCAAGGAC AGTTTACAGG TGAATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813417.1
    CDS754..5283
    Translation

    Target ORF information:

    RefSeq Version XM_012957963.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957963.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGCTTCC TGGTGGTGCC CCTGCTGTGT GGATTGTTAT GCTGTCAGGT GTGGGGATCC 
    CGTCTCCGTC AAGAAGATTT CCCCCCACGG ATAGTTGAGC ATCCATCGGA TGTCATTGTG
    TCAAAGGGAG AGCCAACCAC CTTGAACTGC AAAGCGGAAG GGCGGCCTAC ACCCACCATA
    GAGTGGTACA AAGACTTAGA AAGGGTTGAG ACAGACAAGG ATGACCCCCG CTCACATCGA
    ATGCTACTGC CAAGTGGATC TTTATTTTTT CTGCGCATTA TCCATGGGCG CAAGTTTAAG
    CCAGACGAAG GAAGTTACGT CTGTGTGGCA AGGAACTATC TCGGGGAGGC TATGAGTCGA
    AATGCATCTC TGGAAGTGGC ATTGCTCCGT GATGACTTCC GGCTCAACCC TACAGATGTT
    GTTGTGGCAG CTGGGGAACC TGCAATTTTG GAGTGTCAAC CCCCCAGAGG ACATCCGGAA
    CCAACTATAT ACTGGAAAAA GGACAAAGTT AGAATTGATG ATAAAGATGA AAGAATTAGT
    ATTCGAGGTG GAAAGCTGAT GCTCTCCAAT ACAAGGAAAA GTGATGCAGG CATGTACACT
    TGTGTTGGGA CCAACATGGT TGGAGAAAGA GACAGTGACC CAGCAGAGCT AACAGTCTTT
    GAACGTCCCA CATTCCTCAG AAGGCCAATT AATCAAGTTG TTTTAGAGGA TGAAGCTGTA
    GAATTTCGAT GTCAGGTCCA GGGGGACCCA CAGCCAACTG TCCGGTGGAA AAAGGATGAT
    GTAGATTTGC CAAGAGGAAG ATACGACATC AAGGATGACT ATACTTTGAG AATCAAGAAG
    GCTTCCAGCA GCGACGAAGG GACCTTTGCT TGTATTTCTG AAAACCGGGT TGGTAAAATA
    GAAGCTTCTG CCACACTTAC AGTCAGAGTT CGCCCTGTTG CTCCTCCCCA GTTTGTGATC
    AGACCAAGGG ATCAGATTGT AGCACAAGGC CGGACAGTAA CATTTGGATG TGAAGCTAAA
    GGAAATCCTC AACCAGCTGT TTTTTGGCAA AAAGAAGGAA GCCAGAATTT ACTTTTTCCA
    AATCAGCCTC TTCAGCCCAA CAGCAGGTAC TCAGTGTCAC CAACTGGAGA TTTAACCATC
    ACCAGCATTC AGCGCTCAGA CGCAGGCTAC TACATTTGTC AGGCACTGAC TGTTGCCGGA
    AGCATTCTGG CCAAGGCCCA GCTGGAAGTG ACTGATGTTT TGACAGATAG ACCACCGCCA
    ATAATTCTAC AAGGTCCAGC TAATCAAACT CTTGCCGTGG ATGGAACAGC AGTGCTGAAA
    TGTAAAACTA CTGGGGACCC AATGCCAGTC ATAAGTTGGC TAAAAGAGGG ATTTAAGGGA
    TTCATAGGGA GAGATGCAAG GTTATCCATT CTAGACCAAG GGACATTACA AATTAAAGGT
    TTAAAGGTTA CTGATTCTGG CACCTATACT TGTGTGGCTA CTAGCTCTAG TGGTGAGACA
    ACATGGAGCG CTGTTCTGGA AGTGACAGAA TCTGGTGGTG CCATAATTAG CAAGAACTAT
    GATCTCAATG ACCTTCCTGG ATCTCCATCA AAACCTCAGG TTACAGATGT CAGTAAGAAC
    AGTGTTACCC TATCATGGCA ACCAGGATCA CCAGGGATTC TGCCTGTGAC ATCCTACATC
    ATTGAGGCTT TCAGTCAATC TGTGAGCAAT AGTTGGCAAA CTGTGGCCAA TCACGTGAAG
    ACAACGCTTT ACACTGTTAA AGGACTGCGC CCAAACACCA TTTATCTCTT TATGGTGAGA
    GCAATCAATA CTGAAGGACT GAGTGACCCA AGTCCAATAT CTGATCCAGT GCGTACACAA
    GATATAAGTC CACCTGTTCA AGGAGTAGAT CACCGACAAG TACAGAAAGA ACTTGGGGAT
    GTGATTGTAC GGCTCCATAA TCCTGTTGTT CTGACACCAA CAACAATCCA GGTCACCTGG
    ATGGTCGACC GTCAATCCCA GTTTATTCAG GGATACAGGG TAATGTACCG TCAGACTTCT
    GGTCTGCAGA CTCCATCTGC GTGGCAGAGC TTAGAGGTCA AAGTGCCTGC AGAGCGTAGC
    GCAGTGCTAA CCAACCTGAA AAAAGGAATT ACTTATGAGA TTAAGGTTCG ACCTTATTTT
    AATGAATTTC AAGGAATGGA TAGCGAGTCA AAGTCAGCAC GAACAACTGA AGAAGCCCCA
    AGTGCTCCTC CTCAGTCTGT TACTGTTATG ACAGTGGGAA ATAACAACAG CACCAGCATC
    AGCATCTCAT GGGACCCCCC TCCATCTGAC CACCAGAATG GAATTATCCA GGACTACAGG
    ATCTGGTGCC TGGGTAATGA GACACGTTTC CACATCAACA AGACAGTGGA TGCTGCTATT
    CGTTCCGTGG TTATCGGTGG GCTATTCCCT GGAATTCAGT ACCGCGTTGA AGTCGCAGCC
    AGTACAAGCG CAGGCACTGG GGTAAAAAGT GCTCCCCAGC ACATACAGAT AGGAAATCGG
    AATGAAGTTG TGATCACAGA GAATAACAAC AGCATAACAG AGCAAATAAC TGATGTTGTG
    AAACAGCCGG CATTCATCGC AGGAATTGGT GGTGCCTGCT GGGTAATACT CATGGGATTT
    AGCATTTGGC TCTACTGGCG AAGAAAGAAG AGAAAGGGTC TCAGCAACTA CGCTGTTCAG
    TCTTTCACCT TTACCCCTGC AGTTACATTC CAGAGAGGAG ATGGAGGACT AATGAGTAAT
    GGAAGTCGTC CTGGACTTTT AAATGCGAAT GATCCTAGTT ACCCTTGGTT GGCAGACTCC
    TGGCCAGCTT CCAGCATGCC GGTGAATAAC AGCAACAATG GACCAAACGA TCTCGTTAAT
    TTTAGTTGTG GAGATGTGCT GCCAACAGTT CCAGGTCAAG GAGAAAAAAC AGCGACTATG
    CTTTCTGATG GAGCCATTTA TAGCAGCATT GATTTTACTA CAAAATCTAG CTATAATTGC
    CCAGGACAAA TTGCACAAAC CACGCCATAT GCTACTACGC AGATACTACA TTCCAACAGC
    ATTCATGAGC TGGCAGTTGA CTTGCCTGAT CCACAGTGGA AATCTTCCAT TCAGCAGAAA
    TCTGATCTCA CTGGATTAGG CTATTGCCTT ACTGACCAAG GCAAAGGGAG TAATGCCTTA
    CTTTACATTC CTGACTATCG AATGGCTGAG GGATTGTCAA ATAGAATACC CCACAACCAG
    TCACAGGATT TCAGCACCAC CAGCTCTCAC AACAGCTCAG AGAGAAGTGG CAGCCTTTCA
    GGTGGAAAAG ACGGAAAAAG GAAGAAAAAT AAAAACAGCT CTAAAATCTC TAAAAACAAT
    GGATCCAGTT GGGCCAATGT TCCACTTCCG CCCCCTCCCA TCCAACCACT GCCAGGCACT
    GAAATAGAAC ATTATGGCCT TGAACACCAG GAAACAGGCT ATGACACCAA TGGATGGTGC
    CCACCTTTGC CAGTCCAGAC CTACTTACAT CAAGGATCAG ATGATGAACT AGAAGAAGAT
    GAAGAGAGAG TCCCTACACC CCCTGTACGT GGGGTGGCAT CATCACCTGC CATCTCATTT
    GGACAACAGT CAACAGCAAC CCTCACACCA TCACCACGTG AAGAAATGCA ACCCATGCTT
    CAGGCCCACC TGGATGAATT AACAAGAGCT TACCAGTTTG ACATTGCAAA GCAATCTTGG
    CATTTACACA GCAATAACCA GCCCCCATTG GCCCCAGTTC CACCACTTGG ATATGTGTCT
    GGGACATTTA TTTCTGACTT AGAAAAAGAC ATTCCAGATC AAGAAGAAGA TGAAATTGAA
    GACGAATCTA TAGAAATAAC AAGGCCCCTC CGAGGTTTGG AACACACTCC CGGTTCTAGT
    ATGGATAATC TCGACAGTTC TGTGACAGGC AAAATGAACT CCTTTTCTCA GAGACCCCGG
    CCTGCCAGTC CCTTTTCTAC TGACAGTAAC ACAAGTTCAG CTGTGAATCA TAGTCAGAAG
    TCACGGCCCA CTAAGAAACA CAAGGGAGGC AGAATAGATC AGCAACCCAT GCTTCGCAGA
    GAAGGGATTG CAGATGATTT GCCTCCCCCA CCTGATCCTC CACCTTGTCA AAGACAGCAA
    ATCATGCCCA GTCAACGTGG TGGAGGTCCT ACAGAAAGAA AAGCCAGTTC TCTTGAGAGG
    CACACATCAA ATATGAATGA TTTAAAAACC TCATTAGACC GGCATGGCAG AACATCCTTA
    GAACGGCAGC GACAAACACA GGAATGGCTT GGCTCGACAG AAAGGCAGAA CAAGCAGGCA
    TTTGGATCTG AAGAGATACT AATGCCATAC AGTAAACCTA GCTTCCCATC TCCAGGAGGG
    CACAGCTCCT CAGGAACAGT CTCTTCAAAA GAATCAACTG GACCCAGAAA AGGGGAGGTT
    TTGAGAGGTC ACCAACGTAA TGCCATTGAC TTTCTTGATG TAGGATATTT GGGGCCACAC
    AGTCAAGGAC AGTTTACAGG TGAATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35816
    Clone ID Related Accession (Same CDS sequence) XM_012957964.2
    Accession Version XM_012957964.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4473bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product roundabout homolog 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957964.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGGCTTCC TGGTGGTGCC CCTGCTGTGT GGATTGTTAT GCTGTCAGGT GTGGGGATCC 
    CGTCTCCGTC AAGAAGATTT CCCCCCACGG ATAGTTGAGC ATCCATCGGA TGTCATTGTG
    TCAAAGGGAG AGCCAACCAC CTTGAACTGC AAAGCGGAAG GGCGGCCTAC ACCCACCATA
    GAGTGGTACA AAGACTTAGA AAGGGTTGAG ACAGACAAGG ATGACCCCCG CTCACATCGA
    ATGCTACTGC CAAGTGGATC TTTATTTTTT CTGCGCATTA TCCATGGGCG CAAGTTTAAG
    CCAGACGAAG GAAGTTACGT CTGTGTGGCA AGGAACTATC TCGGGGAGGC TATGAGTCGA
    AATGCATCTC TGGAAGTGGC ATTGCTCCGT GATGACTTCC GGCTCAACCC TACAGATGTT
    GTTGTGGCAG CTGGGGAACC TGCAATTTTG GAGTGTCAAC CCCCCAGAGG ACATCCGGAA
    CCAACTATAT ACTGGAAAAA GGACAAAGTT AGAATTGATG ATAAAGATGA AAGAATTAGT
    ATTCGAGGTG GAAAGCTGAT GCTCTCCAAT ACAAGGAAAA GTGATGCAGG CATGTACACT
    TGTGTTGGGA CCAACATGGT TGGAGAAAGA GACAGTGACC CAGCAGAGCT AACAGTCTTT
    GAACGTCCCA CATTCCTCAG AAGGCCAATT AATCAAGTTG TTTTAGAGGA TGAAGCTGTA
    GAATTTCGAT GTCAGGTCCA GGGGGACCCA CAGCCAACTG TCCGGTGGAA AAAGGATGAT
    GTAGATTTGC CAAGAGGAAG ATACGACATC AAGGATGACT ATACTTTGAG AATCAAGAAG
    GCTTCCAGCA GCGACGAAGG GACCTTTGCT TGTATTTCTG AAAACCGGGT TGGTAAAATA
    GAAGCTTCTG CCACACTTAC AGTCAGAGTT CGCCCTGTTG CTCCTCCCCA GTTTGTGATC
    AGACCAAGGG ATCAGATTGT AGCACAAGGC CGGACAGTAA CATTTGGATG TGAAGCTAAA
    GGAAATCCTC AACCAGCTGT TTTTTGGCAA AAAGAAGGAA GCCAGAATTT ACTTTTTCCA
    AATCAGCCTC TTCAGCCCAA CAGCAGGTAC TCAGTGTCAC CAACTGGAGA TTTAACCATC
    ACCAGCATTC AGCGCTCAGA CGCAGGCTAC TACATTTGTC AGGCACTGAC TGTTGCCGGA
    AGCATTCTGG CCAAGGCCCA GCTGGAAGTG ACTGATGTTT TGACAGATAG ACCACCGCCA
    ATAATTCTAC AAGGTCCAGC TAATCAAACT CTTGCCGTGG ATGGAACAGC AGTGCTGAAA
    TGTAAAACTA CTGGGGACCC AATGCCAGTC ATAAGTTGGC TAAAAGAGGG ATTTAAGGGA
    TTCATAGGGA GAGATGCAAG GTTATCCATT CTAGACCAAG GGACATTACA AATTAAAGGT
    TTAAAGGTTA CTGATTCTGG CACCTATACT TGTGTGGCTA CTAGCTCTAG TGGTGAGACA
    ACATGGAGCG CTGTTCTGGA AGTGACAGAA TCTGGTGGTG CCATAATTAG CAAGAACTAT
    GATCTCAATG ACCTTCCTGG ATCTCCATCA AAACCTCAGG TTACAGATGT CAGTAAGAAC
    AGTGTTACCC TATCATGGCA ACCAGGATCA CCAGGGATTC TGCCTGTGAC ATCCTACATC
    ATTGAGGCTT TCAGTCAATC TGTGAGCAAT AGTTGGCAAA CTGTGGCCAA TCACGTGAAG
    ACAACGCTTT ACACTGTTAA AGGACTGCGC CCAAACACCA TTTATCTCTT TATGGTGAGA
    GCAATCAATA CTGAAGGACT GAGTGACCCA AGTCCAATAT CTGATCCAGT GCGTACACAA
    GATATAAGTC CACCTGTTCA AGGAGTAGAT CACCGACAAG TACAGAAAGA ACTTGGGGAT
    GTGATTGTAC GGCTCCATAA TCCTGTTGTT CTGACACCAA CAACAATCCA GGTCACCTGG
    ATGGTCGACC GTCAATCCCA GTTTATTCAG GGATACAGGG TAATGTACCG TCAGACTTCT
    GGTCTGCAGA CTCCATCTGC GTGGCAGAGC TTAGAGGTCA AAGTGCCTGC AGAGCGTAGC
    GCAGTGCTAA CCAACCTGAA AAAAGGAATT ACTTATGAGA TTAAGGTTCG ACCTTATTTT
    AATGAATTTC AAGGAATGGA TAGCGAGTCA AAGTCAGCAC GAACAACTGA AGAAGCCCCA
    AGTGCTCCTC CTCAGTCTGT TACTGTTATG ACAGTGGGAA ATAACAACAG CACCAGCATC
    AGCATCTCAT GGGACCCCCC TCCATCTGAC CACCAGAATG GAATTATCCA GGACTACAGG
    ATCTGGTGCC TGGGTAATGA GACACGTTTC CACATCAACA AGACAGTGGA TGCTGCTATT
    CGTTCCGTGG TTATCGGTGG GCTATTCCCT GGAATTCAGT ACCGCGTTGA AGTCGCAGCC
    AGTACAAGCG CAGGCACTGG GGTAAAAAGT GCTCCCCAGC ACATACAGAT AGGAAATCGG
    AATGAAGTTG TGATCACAGA GAATAACAAC AGCATAACAG AGCAAATAAC TGATGTTGTG
    AAACAGCCGG CATTCATCGC AGGAATTGGT GGTGCCTGCT GGGTAATACT CATGGGATTT
    AGCATTTGGC TCTACTGGCG AAGAAAGAAG AGAAAGGGTC TCAGCAACTA CGCTGTTCAG
    TCTTTCACCT TTACCCCTGC AGATTCCCCA GATACAACCA TGCACCACAA GGTTGCACTT
    GGCACAAAGA AGGGGAGCTG GCACAAAACA GTTACATTCC AGAGAGGAGA TGGAGGACTA
    ATGAGTAATG GAAGTCGTCC TGGACTTTTA AATGCGAATG ATCCTAGTTA CCCTTGGTTG
    GCAGACTCCT GGCCAGCTTC CAGCATGCCG GTGAATAACA GCAACAATGG ACCAAACGAT
    CTCGTTAATT TTAGTTGTGG AGATGTGCTG CCAACAGTTC CAGGTCAAGG AGAAAAAACA
    GCGACTATGC TTTCTGATGG AGCCATTTAT AGCAGCATTG ATTTTACTAC AAAATCTAGC
    TATAATTGCC CAGGACAAAT TGCACAAACC ACGCCATATG CTACTACGCA GATACTACAT
    TCCAACAGCA TTCATGAGCT GGCAGTTGAC TTGCCTGATC CACAGTGGAA ATCTTCCATT
    CAGCAGAAAT CTGATCTCAC TGGATTAGGC TATTGCCTTA CTGACCAAGG CAAAGGGAGT
    AATGGTGGAA AAGACGGAAA AAGGAAGAAA AATAAAAACA GCTCTAAAAT CTCTAAAAAC
    AATGGATCCA GTTGGGCCAA TGTTCCACTT CCGCCCCCTC CCATCCAACC ACTGCCAGGC
    ACTGAAATAG AACATTATGG CCTTGAACAC CAGGAAACAG GCTATGACAC CAATGGATGG
    TGCCCACCTT TGCCAGTCCA GACCTACTTA CATCAAGGAT CAGATGATGA ACTAGAAGAA
    GATGAAGAGA GAGTCCCTAC ACCCCCTGTA CGTGGGGTGG CATCATCACC TGCCATCTCA
    TTTGGACAAC AGTCAACAGC AACCCTCACA CCATCACCAC GTGAAGAAAT GCAACCCATG
    CTTCAGGCCC ACCTGGATGA ATTAACAAGA GCTTACCAGT TTGACATTGC AAAGCAATCT
    TGGCATTTAC ACAGCAATAA CCAGCCCCCA TTGGCCCCAG TTCCACCACT TGGATATGTG
    TCTGGGACAT TTATTTCTGA CTTAGAAAAA GACATTCCAG ATCAAGAAGA AGATGAAATT
    GAAGACGAAT CTATAGAAAT AACAAGGCCC CTCCGAGGTT TGGAACACAC TCCCGGTTCT
    AGTATGGATA ATCTCGACAG TTCTGTGACA GGCAAAATGA ACTCCTTTTC TCAGAGACCC
    CGGCCTGCCA GTCCCTTTTC TACTGACAGT AACACAAGTT CAGCTGTGAA TCATAGTCAG
    AAGTCACGGC CCACTAAGAA ACACAAGGGA GGCAGAATAG ATCAGCAACC CATGCTTCGC
    AGAGAAGGGA TTGCAGATGA TTTGCCTCCC CCACCTGATC CTCCACCTTG TCAAAGACAG
    CAAATCATGC CCAGTCAACG TGGTGGAGGT CCTACAGAAA GAAAAGCCAG TTCTCTTGAG
    AGGCACACAT CAAATATGAA TGATTTAAAA ACCTCATTAG ACCGGCATGG CAGAACATCC
    TTAGAACGGC AGCGACAAAC ACAGGAATGG CTTGGCTCGA CAGAAAGGCA GAACAAGCAG
    GCATTTGGAT CTGAAGAGAT ACTAATGCCA TACAGTAAAC CTAGCTTCCC ATCTCCAGGA
    GGGCACAGCT CCTCAGGAAC AGTCTCTTCA AAAGAATCAA CTGGACCCAG AAAAGGGGAG
    GTTTTGAGAG GTCACCAACG TAATGCCATT GACTTTCTTG ATGTAGGATA TTTGGGGCCA
    CACAGTCAAG GACAGTTTAC AGGTGAATTA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813418.1
    CDS755..5227
    Translation

    Target ORF information:

    RefSeq Version XM_012957964.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957964.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGGCTTCC TGGTGGTGCC CCTGCTGTGT GGATTGTTAT GCTGTCAGGT GTGGGGATCC 
    CGTCTCCGTC AAGAAGATTT CCCCCCACGG ATAGTTGAGC ATCCATCGGA TGTCATTGTG
    TCAAAGGGAG AGCCAACCAC CTTGAACTGC AAAGCGGAAG GGCGGCCTAC ACCCACCATA
    GAGTGGTACA AAGACTTAGA AAGGGTTGAG ACAGACAAGG ATGACCCCCG CTCACATCGA
    ATGCTACTGC CAAGTGGATC TTTATTTTTT CTGCGCATTA TCCATGGGCG CAAGTTTAAG
    CCAGACGAAG GAAGTTACGT CTGTGTGGCA AGGAACTATC TCGGGGAGGC TATGAGTCGA
    AATGCATCTC TGGAAGTGGC ATTGCTCCGT GATGACTTCC GGCTCAACCC TACAGATGTT
    GTTGTGGCAG CTGGGGAACC TGCAATTTTG GAGTGTCAAC CCCCCAGAGG ACATCCGGAA
    CCAACTATAT ACTGGAAAAA GGACAAAGTT AGAATTGATG ATAAAGATGA AAGAATTAGT
    ATTCGAGGTG GAAAGCTGAT GCTCTCCAAT ACAAGGAAAA GTGATGCAGG CATGTACACT
    TGTGTTGGGA CCAACATGGT TGGAGAAAGA GACAGTGACC CAGCAGAGCT AACAGTCTTT
    GAACGTCCCA CATTCCTCAG AAGGCCAATT AATCAAGTTG TTTTAGAGGA TGAAGCTGTA
    GAATTTCGAT GTCAGGTCCA GGGGGACCCA CAGCCAACTG TCCGGTGGAA AAAGGATGAT
    GTAGATTTGC CAAGAGGAAG ATACGACATC AAGGATGACT ATACTTTGAG AATCAAGAAG
    GCTTCCAGCA GCGACGAAGG GACCTTTGCT TGTATTTCTG AAAACCGGGT TGGTAAAATA
    GAAGCTTCTG CCACACTTAC AGTCAGAGTT CGCCCTGTTG CTCCTCCCCA GTTTGTGATC
    AGACCAAGGG ATCAGATTGT AGCACAAGGC CGGACAGTAA CATTTGGATG TGAAGCTAAA
    GGAAATCCTC AACCAGCTGT TTTTTGGCAA AAAGAAGGAA GCCAGAATTT ACTTTTTCCA
    AATCAGCCTC TTCAGCCCAA CAGCAGGTAC TCAGTGTCAC CAACTGGAGA TTTAACCATC
    ACCAGCATTC AGCGCTCAGA CGCAGGCTAC TACATTTGTC AGGCACTGAC TGTTGCCGGA
    AGCATTCTGG CCAAGGCCCA GCTGGAAGTG ACTGATGTTT TGACAGATAG ACCACCGCCA
    ATAATTCTAC AAGGTCCAGC TAATCAAACT CTTGCCGTGG ATGGAACAGC AGTGCTGAAA
    TGTAAAACTA CTGGGGACCC AATGCCAGTC ATAAGTTGGC TAAAAGAGGG ATTTAAGGGA
    TTCATAGGGA GAGATGCAAG GTTATCCATT CTAGACCAAG GGACATTACA AATTAAAGGT
    TTAAAGGTTA CTGATTCTGG CACCTATACT TGTGTGGCTA CTAGCTCTAG TGGTGAGACA
    ACATGGAGCG CTGTTCTGGA AGTGACAGAA TCTGGTGGTG CCATAATTAG CAAGAACTAT
    GATCTCAATG ACCTTCCTGG ATCTCCATCA AAACCTCAGG TTACAGATGT CAGTAAGAAC
    AGTGTTACCC TATCATGGCA ACCAGGATCA CCAGGGATTC TGCCTGTGAC ATCCTACATC
    ATTGAGGCTT TCAGTCAATC TGTGAGCAAT AGTTGGCAAA CTGTGGCCAA TCACGTGAAG
    ACAACGCTTT ACACTGTTAA AGGACTGCGC CCAAACACCA TTTATCTCTT TATGGTGAGA
    GCAATCAATA CTGAAGGACT GAGTGACCCA AGTCCAATAT CTGATCCAGT GCGTACACAA
    GATATAAGTC CACCTGTTCA AGGAGTAGAT CACCGACAAG TACAGAAAGA ACTTGGGGAT
    GTGATTGTAC GGCTCCATAA TCCTGTTGTT CTGACACCAA CAACAATCCA GGTCACCTGG
    ATGGTCGACC GTCAATCCCA GTTTATTCAG GGATACAGGG TAATGTACCG TCAGACTTCT
    GGTCTGCAGA CTCCATCTGC GTGGCAGAGC TTAGAGGTCA AAGTGCCTGC AGAGCGTAGC
    GCAGTGCTAA CCAACCTGAA AAAAGGAATT ACTTATGAGA TTAAGGTTCG ACCTTATTTT
    AATGAATTTC AAGGAATGGA TAGCGAGTCA AAGTCAGCAC GAACAACTGA AGAAGCCCCA
    AGTGCTCCTC CTCAGTCTGT TACTGTTATG ACAGTGGGAA ATAACAACAG CACCAGCATC
    AGCATCTCAT GGGACCCCCC TCCATCTGAC CACCAGAATG GAATTATCCA GGACTACAGG
    ATCTGGTGCC TGGGTAATGA GACACGTTTC CACATCAACA AGACAGTGGA TGCTGCTATT
    CGTTCCGTGG TTATCGGTGG GCTATTCCCT GGAATTCAGT ACCGCGTTGA AGTCGCAGCC
    AGTACAAGCG CAGGCACTGG GGTAAAAAGT GCTCCCCAGC ACATACAGAT AGGAAATCGG
    AATGAAGTTG TGATCACAGA GAATAACAAC AGCATAACAG AGCAAATAAC TGATGTTGTG
    AAACAGCCGG CATTCATCGC AGGAATTGGT GGTGCCTGCT GGGTAATACT CATGGGATTT
    AGCATTTGGC TCTACTGGCG AAGAAAGAAG AGAAAGGGTC TCAGCAACTA CGCTGTTCAG
    TCTTTCACCT TTACCCCTGC AGATTCCCCA GATACAACCA TGCACCACAA GGTTGCACTT
    GGCACAAAGA AGGGGAGCTG GCACAAAACA GTTACATTCC AGAGAGGAGA TGGAGGACTA
    ATGAGTAATG GAAGTCGTCC TGGACTTTTA AATGCGAATG ATCCTAGTTA CCCTTGGTTG
    GCAGACTCCT GGCCAGCTTC CAGCATGCCG GTGAATAACA GCAACAATGG ACCAAACGAT
    CTCGTTAATT TTAGTTGTGG AGATGTGCTG CCAACAGTTC CAGGTCAAGG AGAAAAAACA
    GCGACTATGC TTTCTGATGG AGCCATTTAT AGCAGCATTG ATTTTACTAC AAAATCTAGC
    TATAATTGCC CAGGACAAAT TGCACAAACC ACGCCATATG CTACTACGCA GATACTACAT
    TCCAACAGCA TTCATGAGCT GGCAGTTGAC TTGCCTGATC CACAGTGGAA ATCTTCCATT
    CAGCAGAAAT CTGATCTCAC TGGATTAGGC TATTGCCTTA CTGACCAAGG CAAAGGGAGT
    AATGGTGGAA AAGACGGAAA AAGGAAGAAA AATAAAAACA GCTCTAAAAT CTCTAAAAAC
    AATGGATCCA GTTGGGCCAA TGTTCCACTT CCGCCCCCTC CCATCCAACC ACTGCCAGGC
    ACTGAAATAG AACATTATGG CCTTGAACAC CAGGAAACAG GCTATGACAC CAATGGATGG
    TGCCCACCTT TGCCAGTCCA GACCTACTTA CATCAAGGAT CAGATGATGA ACTAGAAGAA
    GATGAAGAGA GAGTCCCTAC ACCCCCTGTA CGTGGGGTGG CATCATCACC TGCCATCTCA
    TTTGGACAAC AGTCAACAGC AACCCTCACA CCATCACCAC GTGAAGAAAT GCAACCCATG
    CTTCAGGCCC ACCTGGATGA ATTAACAAGA GCTTACCAGT TTGACATTGC AAAGCAATCT
    TGGCATTTAC ACAGCAATAA CCAGCCCCCA TTGGCCCCAG TTCCACCACT TGGATATGTG
    TCTGGGACAT TTATTTCTGA CTTAGAAAAA GACATTCCAG ATCAAGAAGA AGATGAAATT
    GAAGACGAAT CTATAGAAAT AACAAGGCCC CTCCGAGGTT TGGAACACAC TCCCGGTTCT
    AGTATGGATA ATCTCGACAG TTCTGTGACA GGCAAAATGA ACTCCTTTTC TCAGAGACCC
    CGGCCTGCCA GTCCCTTTTC TACTGACAGT AACACAAGTT CAGCTGTGAA TCATAGTCAG
    AAGTCACGGC CCACTAAGAA ACACAAGGGA GGCAGAATAG ATCAGCAACC CATGCTTCGC
    AGAGAAGGGA TTGCAGATGA TTTGCCTCCC CCACCTGATC CTCCACCTTG TCAAAGACAG
    CAAATCATGC CCAGTCAACG TGGTGGAGGT CCTACAGAAA GAAAAGCCAG TTCTCTTGAG
    AGGCACACAT CAAATATGAA TGATTTAAAA ACCTCATTAG ACCGGCATGG CAGAACATCC
    TTAGAACGGC AGCGACAAAC ACAGGAATGG CTTGGCTCGA CAGAAAGGCA GAACAAGCAG
    GCATTTGGAT CTGAAGAGAT ACTAATGCCA TACAGTAAAC CTAGCTTCCC ATCTCCAGGA
    GGGCACAGCT CCTCAGGAAC AGTCTCTTCA AAAGAATCAA CTGGACCCAG AAAAGGGGAG
    GTTTTGAGAG GTCACCAACG TAATGCCATT GACTTTCTTG ATGTAGGATA TTTGGGGCCA
    CACAGTCAAG GACAGTTTAC AGGTGAATTA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35817
    Clone ID Related Accession (Same CDS sequence) XM_012957965.2
    Accession Version XM_012957965.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4407bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product roundabout homolog 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957965.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGGCTTCC TGGTGGTGCC CCTGCTGTGT GGATTGTTAT GCTGTCAGGT GTGGGGATCC 
    CGTCTCCGTC AAGAAGATTT CCCCCCACGG ATAGTTGAGC ATCCATCGGA TGTCATTGTG
    TCAAAGGGAG AGCCAACCAC CTTGAACTGC AAAGCGGAAG GGCGGCCTAC ACCCACCATA
    GAGTGGTACA AAGACTTAGA AAGGGTTGAG ACAGACAAGG ATGACCCCCG CTCACATCGA
    ATGCTACTGC CAAGTGGATC TTTATTTTTT CTGCGCATTA TCCATGGGCG CAAGTTTAAG
    CCAGACGAAG GAAGTTACGT CTGTGTGGCA AGGAACTATC TCGGGGAGGC TATGAGTCGA
    AATGCATCTC TGGAAGTGGC ATTGCTCCGT GATGACTTCC GGCTCAACCC TACAGATGTT
    GTTGTGGCAG CTGGGGAACC TGCAATTTTG GAGTGTCAAC CCCCCAGAGG ACATCCGGAA
    CCAACTATAT ACTGGAAAAA GGACAAAGTT AGAATTGATG ATAAAGATGA AAGAATTAGT
    ATTCGAGGTG GAAAGCTGAT GCTCTCCAAT ACAAGGAAAA GTGATGCAGG CATGTACACT
    TGTGTTGGGA CCAACATGGT TGGAGAAAGA GACAGTGACC CAGCAGAGCT AACAGTCTTT
    GAACGTCCCA CATTCCTCAG AAGGCCAATT AATCAAGTTG TTTTAGAGGA TGAAGCTGTA
    GAATTTCGAT GTCAGGTCCA GGGGGACCCA CAGCCAACTG TCCGGTGGAA AAAGGATGAT
    GTAGATTTGC CAAGAGGAAG ATACGACATC AAGGATGACT ATACTTTGAG AATCAAGAAG
    GCTTCCAGCA GCGACGAAGG GACCTTTGCT TGTATTTCTG AAAACCGGGT TGGTAAAATA
    GAAGCTTCTG CCACACTTAC AGTCAGAGTT CGCCCTGTTG CTCCTCCCCA GTTTGTGATC
    AGACCAAGGG ATCAGATTGT AGCACAAGGC CGGACAGTAA CATTTGGATG TGAAGCTAAA
    GGAAATCCTC AACCAGCTGT TTTTTGGCAA AAAGAAGGAA GCCAGAATTT ACTTTTTCCA
    AATCAGCCTC TTCAGCCCAA CAGCAGGTAC TCAGTGTCAC CAACTGGAGA TTTAACCATC
    ACCAGCATTC AGCGCTCAGA CGCAGGCTAC TACATTTGTC AGGCACTGAC TGTTGCCGGA
    AGCATTCTGG CCAAGGCCCA GCTGGAAGTG ACTGATGTTT TGACAGATAG ACCACCGCCA
    ATAATTCTAC AAGGTCCAGC TAATCAAACT CTTGCCGTGG ATGGAACAGC AGTGCTGAAA
    TGTAAAACTA CTGGGGACCC AATGCCAGTC ATAAGTTGGC TAAAAGAGGG ATTTAAGGGA
    TTCATAGGGA GAGATGCAAG GTTATCCATT CTAGACCAAG GGACATTACA AATTAAAGGT
    TTAAAGGTTA CTGATTCTGG CACCTATACT TGTGTGGCTA CTAGCTCTAG TGGTGAGACA
    ACATGGAGCG CTGTTCTGGA AGTGACAGAA TCTGGTGGTG CCATAATTAG CAAGAACTAT
    GATCTCAATG ACCTTCCTGG ATCTCCATCA AAACCTCAGG TTACAGATGT CAGTAAGAAC
    AGTGTTACCC TATCATGGCA ACCAGGATCA CCAGGGATTC TGCCTGTGAC ATCCTACATC
    ATTGAGGCTT TCAGTCAATC TGTGAGCAAT AGTTGGCAAA CTGTGGCCAA TCACGTGAAG
    ACAACGCTTT ACACTGTTAA AGGACTGCGC CCAAACACCA TTTATCTCTT TATGGTGAGA
    GCAATCAATA CTGAAGGACT GAGTGACCCA AGTCCAATAT CTGATCCAGT GCGTACACAA
    GATATAAGTC CACCTGTTCA AGGAGTAGAT CACCGACAAG TACAGAAAGA ACTTGGGGAT
    GTGATTGTAC GGCTCCATAA TCCTGTTGTT CTGACACCAA CAACAATCCA GGTCACCTGG
    ATGGTCGACC GTCAATCCCA GTTTATTCAG GGATACAGGG TAATGTACCG TCAGACTTCT
    GGTCTGCAGA CTCCATCTGC GTGGCAGAGC TTAGAGGTCA AAGTGCCTGC AGAGCGTAGC
    GCAGTGCTAA CCAACCTGAA AAAAGGAATT ACTTATGAGA TTAAGGTTCG ACCTTATTTT
    AATGAATTTC AAGGAATGGA TAGCGAGTCA AAGTCAGCAC GAACAACTGA AGAAGCCCCA
    AGTGCTCCTC CTCAGTCTGT TACTGTTATG ACAGTGGGAA ATAACAACAG CACCAGCATC
    AGCATCTCAT GGGACCCCCC TCCATCTGAC CACCAGAATG GAATTATCCA GGACTACAGG
    ATCTGGTGCC TGGGTAATGA GACACGTTTC CACATCAACA AGACAGTGGA TGCTGCTATT
    CGTTCCGTGG TTATCGGTGG GCTATTCCCT GGAATTCAGT ACCGCGTTGA AGTCGCAGCC
    AGTACAAGCG CAGGCACTGG GGTAAAAAGT GCTCCCCAGC ACATACAGAT AGGAAATCGG
    AATGAAGTTG TGATCACAGA GAATAACAAC AGCATAACAG AGCAAATAAC TGATGTTGTG
    AAACAGCCGG CATTCATCGC AGGAATTGGT GGTGCCTGCT GGGTAATACT CATGGGATTT
    AGCATTTGGC TCTACTGGCG AAGAAAGAAG AGAAAGGGTC TCAGCAACTA CGCTGTTCAG
    TCTTTCACCT TTACCCCTGC AGATTCCCCA GATACAACCA TGCACCACAA GGTTGCACTT
    GGCACAAAGA AGGGGAGCTG GCACAAAACA GTTACATTCC AGAGAGGAGA TGGAGGACTA
    ATGAGTAATG GAAGTCGTCC TGGACTTTTA AATGCGAATG ATCCTAGTTA CCCTTGGTTG
    GCAGACTCCT GGCCAGCTTC CAGCATGCCG GTGAATAACA GCAACAATGG ACCAAACGAT
    CTCGTTAATT TTAGTTGTGG AGATGTGCTG CCAACAGTTC CAGGTCAAGG AGAAAAAACA
    GCGACTATGC TTTCTGATGG AGCCATTTAT AGCAGCATTG ATTTTACTAC AAAATCTAGC
    TATAATTGCC CAGGACAAAT TGCACAAACC ACGCCATATG CTACTACGCA GATACTACAT
    TCCAACAGCA TTCATGAGCT GGCAGTTGAC TTGCCTGATC CACAGTGGAA ATCTTCCATT
    CAGCAGAAAT CTGATCTCAC TGGATTAGGC TATTGCCTTA CTGACCAAGG CAAAGGGAGT
    AATGCCTTAC TTTACATTCC TGACTATCGA ATGGCTGAGG GATTGTCAAA TAGAATACCC
    CACAACCAGT CACAGGATTT CAGCACCACC AGCTCTCACA ACAGCTCAGA GAGAAGTGGC
    AGCCTTTCAG GTGGAAAAGA CGGAAAAAGG AAGAAAAATA AAAACAGCTC TAAAATCTCT
    AAAAACAATG GATCCAGTTG GGCCAATGTT CCACTTCCGC CCCCTCCCAT CCAACCACTG
    CCAGGCACTG AAATAGAACA TTATGGCCTT GAACACCAGG AAACAGGCTA TGACACCAAT
    GGATGGTGCC CACCTTTGCC AGTCCAGACC TACTTACATC AAGGATCAGA TGATGAACTA
    GAAGAAGATG AAGAGAGAGT CCCTACACCC CCTGTACGTG GGGTGGCATC ATCACCTGCC
    ATCTCATTTG GACAACAGTC AACAGCAACC CTCACACCAT CACCACGTGA AGAAATGCAA
    CCCATGCTTC AGGCCCACCT GGATGAATTA ACAAGAGCTT ACCAGTTTGA CATTGCAAAG
    CAATCTTGGC ATTTACACAG CAATAACCAG CCCCCATTGG CCCCAGTTCC ACCACTTGGA
    TATGTGTCTG GGACATTTAT TTCTGACTTA GAAAAAGACA TTCCAGATCA AGAAGAAGAT
    GAAATTGAAG ACGAATCTAT AGAAATAACA AGGCCCCTCC GAGGTTTGGA ACACACTCCC
    GGTTCTAGTA TGGATAATCT CGACAGTTCT GTGACAGGCA AAATGAACTC CTTTTCTCAG
    AGACCCCGGC CTGCCAGTCC CTTTTCTACT GACAGTAACA CAAGTTCAGC TGTGAATCAT
    AGTCAGAAGT CACGGCCCAC TAAGAAACAC AAGGGAGGCA GAATAGATCA GCAACCCATG
    CTTCGCAGAG AAGGGATTGC AGATGATTTG CCTCCCCCAC CTGATCCTCC ACCTTGTCAA
    AGACAGCAAA TCATGCCCAG TCAACGTGGT GGAGGTCCTA CAGAAAGAAA AGCCAGTTCT
    CTTGAGAGGC ACACATCAAA TATGAATGAT TTAAAAACCT CATTAGACCG GCATGGCAGA
    ACATCCTTAG AACGGCAGCG ACAAACACAG GAATGGCTTG GCTCGACAGA AAGGCAGAAC
    AAGCAGGCAT TTGGATCTGA GTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813419.1
    CDS756..5162
    Translation

    Target ORF information:

    RefSeq Version XM_012957965.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957965.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGGCTTCC TGGTGGTGCC CCTGCTGTGT GGATTGTTAT GCTGTCAGGT GTGGGGATCC 
    CGTCTCCGTC AAGAAGATTT CCCCCCACGG ATAGTTGAGC ATCCATCGGA TGTCATTGTG
    TCAAAGGGAG AGCCAACCAC CTTGAACTGC AAAGCGGAAG GGCGGCCTAC ACCCACCATA
    GAGTGGTACA AAGACTTAGA AAGGGTTGAG ACAGACAAGG ATGACCCCCG CTCACATCGA
    ATGCTACTGC CAAGTGGATC TTTATTTTTT CTGCGCATTA TCCATGGGCG CAAGTTTAAG
    CCAGACGAAG GAAGTTACGT CTGTGTGGCA AGGAACTATC TCGGGGAGGC TATGAGTCGA
    AATGCATCTC TGGAAGTGGC ATTGCTCCGT GATGACTTCC GGCTCAACCC TACAGATGTT
    GTTGTGGCAG CTGGGGAACC TGCAATTTTG GAGTGTCAAC CCCCCAGAGG ACATCCGGAA
    CCAACTATAT ACTGGAAAAA GGACAAAGTT AGAATTGATG ATAAAGATGA AAGAATTAGT
    ATTCGAGGTG GAAAGCTGAT GCTCTCCAAT ACAAGGAAAA GTGATGCAGG CATGTACACT
    TGTGTTGGGA CCAACATGGT TGGAGAAAGA GACAGTGACC CAGCAGAGCT AACAGTCTTT
    GAACGTCCCA CATTCCTCAG AAGGCCAATT AATCAAGTTG TTTTAGAGGA TGAAGCTGTA
    GAATTTCGAT GTCAGGTCCA GGGGGACCCA CAGCCAACTG TCCGGTGGAA AAAGGATGAT
    GTAGATTTGC CAAGAGGAAG ATACGACATC AAGGATGACT ATACTTTGAG AATCAAGAAG
    GCTTCCAGCA GCGACGAAGG GACCTTTGCT TGTATTTCTG AAAACCGGGT TGGTAAAATA
    GAAGCTTCTG CCACACTTAC AGTCAGAGTT CGCCCTGTTG CTCCTCCCCA GTTTGTGATC
    AGACCAAGGG ATCAGATTGT AGCACAAGGC CGGACAGTAA CATTTGGATG TGAAGCTAAA
    GGAAATCCTC AACCAGCTGT TTTTTGGCAA AAAGAAGGAA GCCAGAATTT ACTTTTTCCA
    AATCAGCCTC TTCAGCCCAA CAGCAGGTAC TCAGTGTCAC CAACTGGAGA TTTAACCATC
    ACCAGCATTC AGCGCTCAGA CGCAGGCTAC TACATTTGTC AGGCACTGAC TGTTGCCGGA
    AGCATTCTGG CCAAGGCCCA GCTGGAAGTG ACTGATGTTT TGACAGATAG ACCACCGCCA
    ATAATTCTAC AAGGTCCAGC TAATCAAACT CTTGCCGTGG ATGGAACAGC AGTGCTGAAA
    TGTAAAACTA CTGGGGACCC AATGCCAGTC ATAAGTTGGC TAAAAGAGGG ATTTAAGGGA
    TTCATAGGGA GAGATGCAAG GTTATCCATT CTAGACCAAG GGACATTACA AATTAAAGGT
    TTAAAGGTTA CTGATTCTGG CACCTATACT TGTGTGGCTA CTAGCTCTAG TGGTGAGACA
    ACATGGAGCG CTGTTCTGGA AGTGACAGAA TCTGGTGGTG CCATAATTAG CAAGAACTAT
    GATCTCAATG ACCTTCCTGG ATCTCCATCA AAACCTCAGG TTACAGATGT CAGTAAGAAC
    AGTGTTACCC TATCATGGCA ACCAGGATCA CCAGGGATTC TGCCTGTGAC ATCCTACATC
    ATTGAGGCTT TCAGTCAATC TGTGAGCAAT AGTTGGCAAA CTGTGGCCAA TCACGTGAAG
    ACAACGCTTT ACACTGTTAA AGGACTGCGC CCAAACACCA TTTATCTCTT TATGGTGAGA
    GCAATCAATA CTGAAGGACT GAGTGACCCA AGTCCAATAT CTGATCCAGT GCGTACACAA
    GATATAAGTC CACCTGTTCA AGGAGTAGAT CACCGACAAG TACAGAAAGA ACTTGGGGAT
    GTGATTGTAC GGCTCCATAA TCCTGTTGTT CTGACACCAA CAACAATCCA GGTCACCTGG
    ATGGTCGACC GTCAATCCCA GTTTATTCAG GGATACAGGG TAATGTACCG TCAGACTTCT
    GGTCTGCAGA CTCCATCTGC GTGGCAGAGC TTAGAGGTCA AAGTGCCTGC AGAGCGTAGC
    GCAGTGCTAA CCAACCTGAA AAAAGGAATT ACTTATGAGA TTAAGGTTCG ACCTTATTTT
    AATGAATTTC AAGGAATGGA TAGCGAGTCA AAGTCAGCAC GAACAACTGA AGAAGCCCCA
    AGTGCTCCTC CTCAGTCTGT TACTGTTATG ACAGTGGGAA ATAACAACAG CACCAGCATC
    AGCATCTCAT GGGACCCCCC TCCATCTGAC CACCAGAATG GAATTATCCA GGACTACAGG
    ATCTGGTGCC TGGGTAATGA GACACGTTTC CACATCAACA AGACAGTGGA TGCTGCTATT
    CGTTCCGTGG TTATCGGTGG GCTATTCCCT GGAATTCAGT ACCGCGTTGA AGTCGCAGCC
    AGTACAAGCG CAGGCACTGG GGTAAAAAGT GCTCCCCAGC ACATACAGAT AGGAAATCGG
    AATGAAGTTG TGATCACAGA GAATAACAAC AGCATAACAG AGCAAATAAC TGATGTTGTG
    AAACAGCCGG CATTCATCGC AGGAATTGGT GGTGCCTGCT GGGTAATACT CATGGGATTT
    AGCATTTGGC TCTACTGGCG AAGAAAGAAG AGAAAGGGTC TCAGCAACTA CGCTGTTCAG
    TCTTTCACCT TTACCCCTGC AGATTCCCCA GATACAACCA TGCACCACAA GGTTGCACTT
    GGCACAAAGA AGGGGAGCTG GCACAAAACA GTTACATTCC AGAGAGGAGA TGGAGGACTA
    ATGAGTAATG GAAGTCGTCC TGGACTTTTA AATGCGAATG ATCCTAGTTA CCCTTGGTTG
    GCAGACTCCT GGCCAGCTTC CAGCATGCCG GTGAATAACA GCAACAATGG ACCAAACGAT
    CTCGTTAATT TTAGTTGTGG AGATGTGCTG CCAACAGTTC CAGGTCAAGG AGAAAAAACA
    GCGACTATGC TTTCTGATGG AGCCATTTAT AGCAGCATTG ATTTTACTAC AAAATCTAGC
    TATAATTGCC CAGGACAAAT TGCACAAACC ACGCCATATG CTACTACGCA GATACTACAT
    TCCAACAGCA TTCATGAGCT GGCAGTTGAC TTGCCTGATC CACAGTGGAA ATCTTCCATT
    CAGCAGAAAT CTGATCTCAC TGGATTAGGC TATTGCCTTA CTGACCAAGG CAAAGGGAGT
    AATGCCTTAC TTTACATTCC TGACTATCGA ATGGCTGAGG GATTGTCAAA TAGAATACCC
    CACAACCAGT CACAGGATTT CAGCACCACC AGCTCTCACA ACAGCTCAGA GAGAAGTGGC
    AGCCTTTCAG GTGGAAAAGA CGGAAAAAGG AAGAAAAATA AAAACAGCTC TAAAATCTCT
    AAAAACAATG GATCCAGTTG GGCCAATGTT CCACTTCCGC CCCCTCCCAT CCAACCACTG
    CCAGGCACTG AAATAGAACA TTATGGCCTT GAACACCAGG AAACAGGCTA TGACACCAAT
    GGATGGTGCC CACCTTTGCC AGTCCAGACC TACTTACATC AAGGATCAGA TGATGAACTA
    GAAGAAGATG AAGAGAGAGT CCCTACACCC CCTGTACGTG GGGTGGCATC ATCACCTGCC
    ATCTCATTTG GACAACAGTC AACAGCAACC CTCACACCAT CACCACGTGA AGAAATGCAA
    CCCATGCTTC AGGCCCACCT GGATGAATTA ACAAGAGCTT ACCAGTTTGA CATTGCAAAG
    CAATCTTGGC ATTTACACAG CAATAACCAG CCCCCATTGG CCCCAGTTCC ACCACTTGGA
    TATGTGTCTG GGACATTTAT TTCTGACTTA GAAAAAGACA TTCCAGATCA AGAAGAAGAT
    GAAATTGAAG ACGAATCTAT AGAAATAACA AGGCCCCTCC GAGGTTTGGA ACACACTCCC
    GGTTCTAGTA TGGATAATCT CGACAGTTCT GTGACAGGCA AAATGAACTC CTTTTCTCAG
    AGACCCCGGC CTGCCAGTCC CTTTTCTACT GACAGTAACA CAAGTTCAGC TGTGAATCAT
    AGTCAGAAGT CACGGCCCAC TAAGAAACAC AAGGGAGGCA GAATAGATCA GCAACCCATG
    CTTCGCAGAG AAGGGATTGC AGATGATTTG CCTCCCCCAC CTGATCCTCC ACCTTGTCAA
    AGACAGCAAA TCATGCCCAG TCAACGTGGT GGAGGTCCTA CAGAAAGAAA AGCCAGTTCT
    CTTGAGAGGC ACACATCAAA TATGAATGAT TTAAAAACCT CATTAGACCG GCATGGCAGA
    ACATCCTTAG AACGGCAGCG ACAAACACAG GAATGGCTTG GCTCGACAGA AAGGCAGAAC
    AAGCAGGCAT TTGGATCTGA GTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35818
    Clone ID Related Accession (Same CDS sequence) XM_012957966.2
    Accession Version XM_012957966.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4377bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product roundabout homolog 2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957966.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGGCTTCC TGGTGGTGCC CCTGCTGTGT GGATTGTTAT GCTGTCAGGT GTGGGGATCC 
    CGTCTCCGTC AAGAAGATTT CCCCCCACGG ATAGTTGAGC ATCCATCGGA TGTCATTGTG
    TCAAAGGGAG AGCCAACCAC CTTGAACTGC AAAGCGGAAG GGCGGCCTAC ACCCACCATA
    GAGTGGTACA AAGACTTAGA AAGGGTTGAG ACAGACAAGG ATGACCCCCG CTCACATCGA
    ATGCTACTGC CAAGTGGATC TTTATTTTTT CTGCGCATTA TCCATGGGCG CAAGTTTAAG
    CCAGACGAAG GAAGTTACGT CTGTGTGGCA AGGAACTATC TCGGGGAGGC TATGAGTCGA
    AATGCATCTC TGGAAGTGGC ATTGCTCCGT GATGACTTCC GGCTCAACCC TACAGATGTT
    GTTGTGGCAG CTGGGGAACC TGCAATTTTG GAGTGTCAAC CCCCCAGAGG ACATCCGGAA
    CCAACTATAT ACTGGAAAAA GGACAAAGTT AGAATTGATG ATAAAGATGA AAGAATTAGT
    ATTCGAGGTG GAAAGCTGAT GCTCTCCAAT ACAAGGAAAA GTGATGCAGG CATGTACACT
    TGTGTTGGGA CCAACATGGT TGGAGAAAGA GACAGTGACC CAGCAGAGCT AACAGTCTTT
    GAACGTCCCA CATTCCTCAG AAGGCCAATT AATCAAGTTG TTTTAGAGGA TGAAGCTGTA
    GAATTTCGAT GTCAGGTCCA GGGGGACCCA CAGCCAACTG TCCGGTGGAA AAAGGATGAT
    GTAGATTTGC CAAGAGGAAG ATACGACATC AAGGATGACT ATACTTTGAG AATCAAGAAG
    GCTTCCAGCA GCGACGAAGG GACCTTTGCT TGTATTTCTG AAAACCGGGT TGGTAAAATA
    GAAGCTTCTG CCACACTTAC AGTCAGAGTT CGCCCTGTTG CTCCTCCCCA GTTTGTGATC
    AGACCAAGGG ATCAGATTGT AGCACAAGGC CGGACAGTAA CATTTGGATG TGAAGCTAAA
    GGAAATCCTC AACCAGCTGT TTTTTGGCAA AAAGAAGGAA GCCAGAATTT ACTTTTTCCA
    AATCAGCCTC TTCAGCCCAA CAGCAGGTAC TCAGTGTCAC CAACTGGAGA TTTAACCATC
    ACCAGCATTC AGCGCTCAGA CGCAGGCTAC TACATTTGTC AGGCACTGAC TGTTGCCGGA
    AGCATTCTGG CCAAGGCCCA GCTGGAAGTG ACTGATGTTT TGACAGATAG ACCACCGCCA
    ATAATTCTAC AAGGTCCAGC TAATCAAACT CTTGCCGTGG ATGGAACAGC AGTGCTGAAA
    TGTAAAACTA CTGGGGACCC AATGCCAGTC ATAAGTTGGC TAAAAGAGGG ATTTAAGGGA
    TTCATAGGGA GAGATGCAAG GTTATCCATT CTAGACCAAG GGACATTACA AATTAAAGGT
    TTAAAGGTTA CTGATTCTGG CACCTATACT TGTGTGGCTA CTAGCTCTAG TGGTGAGACA
    ACATGGAGCG CTGTTCTGGA AGTGACAGAA TCTGGTGGTG CCATAATTAG CAAGAACTAT
    GATCTCAATG ACCTTCCTGG ATCTCCATCA AAACCTCAGG TTACAGATGT CAGTAAGAAC
    AGTGTTACCC TATCATGGCA ACCAGGATCA CCAGGGATTC TGCCTGTGAC ATCCTACATC
    ATTGAGGCTT TCAGTCAATC TGTGAGCAAT AGTTGGCAAA CTGTGGCCAA TCACGTGAAG
    ACAACGCTTT ACACTGTTAA AGGACTGCGC CCAAACACCA TTTATCTCTT TATGGTGAGA
    GCAATCAATA CTGAAGGACT GAGTGACCCA AGTCCAATAT CTGATCCAGT GCGTACACAA
    GATATAAGTC CACCTGTTCA AGGAGTAGAT CACCGACAAG TACAGAAAGA ACTTGGGGAT
    GTGATTGTAC GGCTCCATAA TCCTGTTGTT CTGACACCAA CAACAATCCA GGTCACCTGG
    ATGGTCGACC GTCAATCCCA GTTTATTCAG GGATACAGGG TAATGTACCG TCAGACTTCT
    GGTCTGCAGA CTCCATCTGC GTGGCAGAGC TTAGAGGTCA AAGTGCCTGC AGAGCGTAGC
    GCAGTGCTAA CCAACCTGAA AAAAGGAATT ACTTATGAGA TTAAGGTTCG ACCTTATTTT
    AATGAATTTC AAGGAATGGA TAGCGAGTCA AAGTCAGCAC GAACAACTGA AGAAGCCCCA
    AGTGCTCCTC CTCAGTCTGT TACTGTTATG ACAGTGGGAA ATAACAACAG CACCAGCATC
    AGCATCTCAT GGGACCCCCC TCCATCTGAC CACCAGAATG GAATTATCCA GGACTACAGG
    ATCTGGTGCC TGGGTAATGA GACACGTTTC CACATCAACA AGACAGTGGA TGCTGCTATT
    CGTTCCGTGG TTATCGGTGG GCTATTCCCT GGAATTCAGT ACCGCGTTGA AGTCGCAGCC
    AGTACAAGCG CAGGCACTGG GGTAAAAAGT GCTCCCCAGC ACATACAGAT AGGAAATCGG
    AATGAAGTTG TGATCACAGA GAATAACAAC AGCATAACAG AGCAAATAAC TGATGTTGTG
    AAACAGCCGG CATTCATCGC AGGAATTGGT GGTGCCTGCT GGGTAATACT CATGGGATTT
    AGCATTTGGC TCTACTGGCG AAGAAAGAAG AGAAAGGGTC TCAGCAACTA CGCTGTTACA
    TTCCAGAGAG GAGATGGAGG ACTAATGAGT AATGGAAGTC GTCCTGGACT TTTAAATGCG
    AATGATCCTA GTTACCCTTG GTTGGCAGAC TCCTGGCCAG CTTCCAGCAT GCCGGTGAAT
    AACAGCAACA ATGGACCAAA CGATCTCGTT AATTTTAGTT GTGGAGATGT GCTGCCAACA
    GTTCCAGGTC AAGGAGAAAA AACAGCGACT ATGCTTTCTG ATGGAGCCAT TTATAGCAGC
    ATTGATTTTA CTACAAAATC TAGCTATAAT TGCCCAGGAC AAATTGCACA AACCACGCCA
    TATGCTACTA CGCAGATACT ACATTCCAAC AGCATTCATG AGCTGGCAGT TGACTTGCCT
    GATCCACAGT GGAAATCTTC CATTCAGCAG AAATCTGATC TCACTGGATT AGGCTATTGC
    CTTACTGACC AAGGCAAAGG GAGTAATGGT GGAAAAGACG GAAAAAGGAA GAAAAATAAA
    AACAGCTCTA AAATCTCTAA AAACAATGGA TCCAGTTGGG CCAATGTTCC ACTTCCGCCC
    CCTCCCATCC AACCACTGCC AGGCACTGAA ATAGAACATT ATGGCCTTGA ACACCAGGAA
    ACAGGCTATG ACACCAATGG ATGGTGCCCA CCTTTGCCAG TCCAGACCTA CTTACATCAA
    GGATCAGATG ATGAACTAGA AGAAGATGAA GAGAGAGTCC CTACACCCCC TGTACGTGGG
    GTGGCATCAT CACCTGCCAT CTCATTTGGA CAACAGTCAA CAGCAACCCT CACACCATCA
    CCACGTGAAG AAATGCAACC CATGCTTCAG GCCCACCTGG ATGAATTAAC AAGAGCTTAC
    CAGTTTGACA TTGCAAAGCA ATCTTGGCAT TTACACAGCA ATAACCAGCC CCCATTGGCC
    CCAGTTCCAC CACTTGGATA TGTGTCTGGG ACATTTATTT CTGACTTAGA AAAAGACATT
    CCAGATCAAG AAGAAGATGA AATTGAAGAC GAATCTATAG AAATAACAAG GCCCCTCCGA
    GGTTTGGAAC ACACTCCCGG TTCTAGTATG GATAATCTCG ACAGTTCTGT GACAGGCAAA
    ATGAACTCCT TTTCTCAGAG ACCCCGGCCT GCCAGTCCCT TTTCTACTGA CAGTAACACA
    AGTTCAGCTG TGAATCATAG TCAGAAGTCA CGGCCCACTA AGAAACACAA GGGAGGCAGA
    ATAGATCAGC AACCCATGCT TCGCAGAGAA GGGATTGCAG ATGATTTGCC TCCCCCACCT
    GATCCTCCAC CTTGTCAAAG ACAGCAAATC ATGCCCAGTC AACGTGGTGG AGGTCCTACA
    GAAAGAAAAG CCAGTTCTCT TGAGAGGCAC ACATCAAATA TGAATGATTT AAAAACCTCA
    TTAGACCGGC ATGGCAGAAC ATCCTTAGAA CGGCAGCGAC AAACACAGGA ATGGCTTGGC
    TCGACAGAAA GGCAGAACAA GCAGGCATTT GGATCTGAAG AGATACTAAT GCCATACAGT
    AAACCTAGCT TCCCATCTCC AGGAGGGCAC AGCTCCTCAG GAACAGTCTC TTCAAAAGAA
    TCAACTGGAC CCAGAAAAGG GGAGGTTTTG AGAGGTCACC AACGTAATGC CATTGACTTT
    CTTGATGTAG GATATTTGGG GCCACACAGT CAAGGACAGT TTACAGGTGA ATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813420.1
    CDS754..5130
    Translation

    Target ORF information:

    RefSeq Version XM_012957966.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957966.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGGCTTCC TGGTGGTGCC CCTGCTGTGT GGATTGTTAT GCTGTCAGGT GTGGGGATCC 
    CGTCTCCGTC AAGAAGATTT CCCCCCACGG ATAGTTGAGC ATCCATCGGA TGTCATTGTG
    TCAAAGGGAG AGCCAACCAC CTTGAACTGC AAAGCGGAAG GGCGGCCTAC ACCCACCATA
    GAGTGGTACA AAGACTTAGA AAGGGTTGAG ACAGACAAGG ATGACCCCCG CTCACATCGA
    ATGCTACTGC CAAGTGGATC TTTATTTTTT CTGCGCATTA TCCATGGGCG CAAGTTTAAG
    CCAGACGAAG GAAGTTACGT CTGTGTGGCA AGGAACTATC TCGGGGAGGC TATGAGTCGA
    AATGCATCTC TGGAAGTGGC ATTGCTCCGT GATGACTTCC GGCTCAACCC TACAGATGTT
    GTTGTGGCAG CTGGGGAACC TGCAATTTTG GAGTGTCAAC CCCCCAGAGG ACATCCGGAA
    CCAACTATAT ACTGGAAAAA GGACAAAGTT AGAATTGATG ATAAAGATGA AAGAATTAGT
    ATTCGAGGTG GAAAGCTGAT GCTCTCCAAT ACAAGGAAAA GTGATGCAGG CATGTACACT
    TGTGTTGGGA CCAACATGGT TGGAGAAAGA GACAGTGACC CAGCAGAGCT AACAGTCTTT
    GAACGTCCCA CATTCCTCAG AAGGCCAATT AATCAAGTTG TTTTAGAGGA TGAAGCTGTA
    GAATTTCGAT GTCAGGTCCA GGGGGACCCA CAGCCAACTG TCCGGTGGAA AAAGGATGAT
    GTAGATTTGC CAAGAGGAAG ATACGACATC AAGGATGACT ATACTTTGAG AATCAAGAAG
    GCTTCCAGCA GCGACGAAGG GACCTTTGCT TGTATTTCTG AAAACCGGGT TGGTAAAATA
    GAAGCTTCTG CCACACTTAC AGTCAGAGTT CGCCCTGTTG CTCCTCCCCA GTTTGTGATC
    AGACCAAGGG ATCAGATTGT AGCACAAGGC CGGACAGTAA CATTTGGATG TGAAGCTAAA
    GGAAATCCTC AACCAGCTGT TTTTTGGCAA AAAGAAGGAA GCCAGAATTT ACTTTTTCCA
    AATCAGCCTC TTCAGCCCAA CAGCAGGTAC TCAGTGTCAC CAACTGGAGA TTTAACCATC
    ACCAGCATTC AGCGCTCAGA CGCAGGCTAC TACATTTGTC AGGCACTGAC TGTTGCCGGA
    AGCATTCTGG CCAAGGCCCA GCTGGAAGTG ACTGATGTTT TGACAGATAG ACCACCGCCA
    ATAATTCTAC AAGGTCCAGC TAATCAAACT CTTGCCGTGG ATGGAACAGC AGTGCTGAAA
    TGTAAAACTA CTGGGGACCC AATGCCAGTC ATAAGTTGGC TAAAAGAGGG ATTTAAGGGA
    TTCATAGGGA GAGATGCAAG GTTATCCATT CTAGACCAAG GGACATTACA AATTAAAGGT
    TTAAAGGTTA CTGATTCTGG CACCTATACT TGTGTGGCTA CTAGCTCTAG TGGTGAGACA
    ACATGGAGCG CTGTTCTGGA AGTGACAGAA TCTGGTGGTG CCATAATTAG CAAGAACTAT
    GATCTCAATG ACCTTCCTGG ATCTCCATCA AAACCTCAGG TTACAGATGT CAGTAAGAAC
    AGTGTTACCC TATCATGGCA ACCAGGATCA CCAGGGATTC TGCCTGTGAC ATCCTACATC
    ATTGAGGCTT TCAGTCAATC TGTGAGCAAT AGTTGGCAAA CTGTGGCCAA TCACGTGAAG
    ACAACGCTTT ACACTGTTAA AGGACTGCGC CCAAACACCA TTTATCTCTT TATGGTGAGA
    GCAATCAATA CTGAAGGACT GAGTGACCCA AGTCCAATAT CTGATCCAGT GCGTACACAA
    GATATAAGTC CACCTGTTCA AGGAGTAGAT CACCGACAAG TACAGAAAGA ACTTGGGGAT
    GTGATTGTAC GGCTCCATAA TCCTGTTGTT CTGACACCAA CAACAATCCA GGTCACCTGG
    ATGGTCGACC GTCAATCCCA GTTTATTCAG GGATACAGGG TAATGTACCG TCAGACTTCT
    GGTCTGCAGA CTCCATCTGC GTGGCAGAGC TTAGAGGTCA AAGTGCCTGC AGAGCGTAGC
    GCAGTGCTAA CCAACCTGAA AAAAGGAATT ACTTATGAGA TTAAGGTTCG ACCTTATTTT
    AATGAATTTC AAGGAATGGA TAGCGAGTCA AAGTCAGCAC GAACAACTGA AGAAGCCCCA
    AGTGCTCCTC CTCAGTCTGT TACTGTTATG ACAGTGGGAA ATAACAACAG CACCAGCATC
    AGCATCTCAT GGGACCCCCC TCCATCTGAC CACCAGAATG GAATTATCCA GGACTACAGG
    ATCTGGTGCC TGGGTAATGA GACACGTTTC CACATCAACA AGACAGTGGA TGCTGCTATT
    CGTTCCGTGG TTATCGGTGG GCTATTCCCT GGAATTCAGT ACCGCGTTGA AGTCGCAGCC
    AGTACAAGCG CAGGCACTGG GGTAAAAAGT GCTCCCCAGC ACATACAGAT AGGAAATCGG
    AATGAAGTTG TGATCACAGA GAATAACAAC AGCATAACAG AGCAAATAAC TGATGTTGTG
    AAACAGCCGG CATTCATCGC AGGAATTGGT GGTGCCTGCT GGGTAATACT CATGGGATTT
    AGCATTTGGC TCTACTGGCG AAGAAAGAAG AGAAAGGGTC TCAGCAACTA CGCTGTTACA
    TTCCAGAGAG GAGATGGAGG ACTAATGAGT AATGGAAGTC GTCCTGGACT TTTAAATGCG
    AATGATCCTA GTTACCCTTG GTTGGCAGAC TCCTGGCCAG CTTCCAGCAT GCCGGTGAAT
    AACAGCAACA ATGGACCAAA CGATCTCGTT AATTTTAGTT GTGGAGATGT GCTGCCAACA
    GTTCCAGGTC AAGGAGAAAA AACAGCGACT ATGCTTTCTG ATGGAGCCAT TTATAGCAGC
    ATTGATTTTA CTACAAAATC TAGCTATAAT TGCCCAGGAC AAATTGCACA AACCACGCCA
    TATGCTACTA CGCAGATACT ACATTCCAAC AGCATTCATG AGCTGGCAGT TGACTTGCCT
    GATCCACAGT GGAAATCTTC CATTCAGCAG AAATCTGATC TCACTGGATT AGGCTATTGC
    CTTACTGACC AAGGCAAAGG GAGTAATGGT GGAAAAGACG GAAAAAGGAA GAAAAATAAA
    AACAGCTCTA AAATCTCTAA AAACAATGGA TCCAGTTGGG CCAATGTTCC ACTTCCGCCC
    CCTCCCATCC AACCACTGCC AGGCACTGAA ATAGAACATT ATGGCCTTGA ACACCAGGAA
    ACAGGCTATG ACACCAATGG ATGGTGCCCA CCTTTGCCAG TCCAGACCTA CTTACATCAA
    GGATCAGATG ATGAACTAGA AGAAGATGAA GAGAGAGTCC CTACACCCCC TGTACGTGGG
    GTGGCATCAT CACCTGCCAT CTCATTTGGA CAACAGTCAA CAGCAACCCT CACACCATCA
    CCACGTGAAG AAATGCAACC CATGCTTCAG GCCCACCTGG ATGAATTAAC AAGAGCTTAC
    CAGTTTGACA TTGCAAAGCA ATCTTGGCAT TTACACAGCA ATAACCAGCC CCCATTGGCC
    CCAGTTCCAC CACTTGGATA TGTGTCTGGG ACATTTATTT CTGACTTAGA AAAAGACATT
    CCAGATCAAG AAGAAGATGA AATTGAAGAC GAATCTATAG AAATAACAAG GCCCCTCCGA
    GGTTTGGAAC ACACTCCCGG TTCTAGTATG GATAATCTCG ACAGTTCTGT GACAGGCAAA
    ATGAACTCCT TTTCTCAGAG ACCCCGGCCT GCCAGTCCCT TTTCTACTGA CAGTAACACA
    AGTTCAGCTG TGAATCATAG TCAGAAGTCA CGGCCCACTA AGAAACACAA GGGAGGCAGA
    ATAGATCAGC AACCCATGCT TCGCAGAGAA GGGATTGCAG ATGATTTGCC TCCCCCACCT
    GATCCTCCAC CTTGTCAAAG ACAGCAAATC ATGCCCAGTC AACGTGGTGG AGGTCCTACA
    GAAAGAAAAG CCAGTTCTCT TGAGAGGCAC ACATCAAATA TGAATGATTT AAAAACCTCA
    TTAGACCGGC ATGGCAGAAC ATCCTTAGAA CGGCAGCGAC AAACACAGGA ATGGCTTGGC
    TCGACAGAAA GGCAGAACAA GCAGGCATTT GGATCTGAAG AGATACTAAT GCCATACAGT
    AAACCTAGCT TCCCATCTCC AGGAGGGCAC AGCTCCTCAG GAACAGTCTC TTCAAAAGAA
    TCAACTGGAC CCAGAAAAGG GGAGGTTTTG AGAGGTCACC AACGTAATGC CATTGACTTT
    CTTGATGTAG GATATTTGGG GCCACACAGT CAAGGACAGT TTACAGGTGA ATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35819
    Clone ID Related Accession (Same CDS sequence) XM_012957967.2
    Accession Version XM_012957967.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4365bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product roundabout homolog 2 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957967.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGGCTTCC TGGTGGTGCC CCTGCTGTGT GGATTGTTAT GCTGTCAGGT GTGGGGATCC 
    CGTCTCCGTC AAGAAGATTT CCCCCCACGG ATAGTTGAGC ATCCATCGGA TGTCATTGTG
    TCAAAGGGAG AGCCAACCAC CTTGAACTGC AAAGCGGAAG GGCGGCCTAC ACCCACCATA
    GAGTGGTACA AAGACTTAGA AAGGGTTGAG ACAGACAAGG ATGACCCCCG CTCACATCGA
    ATGCTACTGC CAAGTGGATC TTTATTTTTT CTGCGCATTA TCCATGGGCG CAAGTTTAAG
    CCAGACGAAG GAAGTTACGT CTGTGTGGCA AGGAACTATC TCGGGGAGGC TATGAGTCGA
    AATGCATCTC TGGAAGTGGC ATTGCTCCGT GATGACTTCC GGCTCAACCC TACAGATGTT
    GTTGTGGCAG CTGGGGAACC TGCAATTTTG GAGTGTCAAC CCCCCAGAGG ACATCCGGAA
    CCAACTATAT ACTGGAAAAA GGACAAAGTT AGAATTGATG ATAAAGATGA AAGAATTAGT
    ATTCGAGGTG GAAAGCTGAT GCTCTCCAAT ACAAGGAAAA GTGATGCAGG CATGTACACT
    TGTGTTGGGA CCAACATGGT TGGAGAAAGA GACAGTGACC CAGCAGAGCT AACAGTCTTT
    GAACGTCCCA CATTCCTCAG AAGGCCAATT AATCAAGTTG TTTTAGAGGA TGAAGCTGTA
    GAATTTCGAT GTCAGGTCCA GGGGGACCCA CAGCCAACTG TCCGGTGGAA AAAGGATGAT
    GTAGATTTGC CAAGAGGAAG ATACGACATC AAGGATGACT ATACTTTGAG AATCAAGAAG
    GCTTCCAGCA GCGACGAAGG GACCTTTGCT TGTATTTCTG AAAACCGGGT TGGTAAAATA
    GAAGCTTCTG CCACACTTAC AGTCAGAGCT CCTCCCCAGT TTGTGATCAG ACCAAGGGAT
    CAGATTGTAG CACAAGGCCG GACAGTAACA TTTGGATGTG AAGCTAAAGG AAATCCTCAA
    CCAGCTGTTT TTTGGCAAAA AGAAGGAAGC CAGAATTTAC TTTTTCCAAA TCAGCCTCTT
    CAGCCCAACA GCAGGTACTC AGTGTCACCA ACTGGAGATT TAACCATCAC CAGCATTCAG
    CGCTCAGACG CAGGCTACTA CATTTGTCAG GCACTGACTG TTGCCGGAAG CATTCTGGCC
    AAGGCCCAGC TGGAAGTGAC TGATGTTTTG ACAGATAGAC CACCGCCAAT AATTCTACAA
    GGTCCAGCTA ATCAAACTCT TGCCGTGGAT GGAACAGCAG TGCTGAAATG TAAAACTACT
    GGGGACCCAA TGCCAGTCAT AAGTTGGCTA AAAGAGGGAT TTAAGGGATT CATAGGGAGA
    GATGCAAGGT TATCCATTCT AGACCAAGGG ACATTACAAA TTAAAGGTTT AAAGGTTACT
    GATTCTGGCA CCTATACTTG TGTGGCTACT AGCTCTAGTG GTGAGACAAC ATGGAGCGCT
    GTTCTGGAAG TGACAGAATC TGGTGGTGCC ATAATTAGCA AGAACTATGA TCTCAATGAC
    CTTCCTGGAT CTCCATCAAA ACCTCAGGTT ACAGATGTCA GTAAGAACAG TGTTACCCTA
    TCATGGCAAC CAGGATCACC AGGGATTCTG CCTGTGACAT CCTACATCAT TGAGGCTTTC
    AGTCAATCTG TGAGCAATAG TTGGCAAACT GTGGCCAATC ACGTGAAGAC AACGCTTTAC
    ACTGTTAAAG GACTGCGCCC AAACACCATT TATCTCTTTA TGGTGAGAGC AATCAATACT
    GAAGGACTGA GTGACCCAAG TCCAATATCT GATCCAGTGC GTACACAAGA TATAAGTCCA
    CCTGTTCAAG GAGTAGATCA CCGACAAGTA CAGAAAGAAC TTGGGGATGT GATTGTACGG
    CTCCATAATC CTGTTGTTCT GACACCAACA ACAATCCAGG TCACCTGGAT GGTCGACCGT
    CAATCCCAGT TTATTCAGGG ATACAGGGTA ATGTACCGTC AGACTTCTGG TCTGCAGACT
    CCATCTGCGT GGCAGAGCTT AGAGGTCAAA GTGCCTGCAG AGCGTAGCGC AGTGCTAACC
    AACCTGAAAA AAGGAATTAC TTATGAGATT AAGGTTCGAC CTTATTTTAA TGAATTTCAA
    GGAATGGATA GCGAGTCAAA GTCAGCACGA ACAACTGAAG AAGCCCCAAG TGCTCCTCCT
    CAGTCTGTTA CTGTTATGAC AGTGGGAAAT AACAACAGCA CCAGCATCAG CATCTCATGG
    GACCCCCCTC CATCTGACCA CCAGAATGGA ATTATCCAGG ACTACAGGAT CTGGTGCCTG
    GGTAATGAGA CACGTTTCCA CATCAACAAG ACAGTGGATG CTGCTATTCG TTCCGTGGTT
    ATCGGTGGGC TATTCCCTGG AATTCAGTAC CGCGTTGAAG TCGCAGCCAG TACAAGCGCA
    GGCACTGGGG TAAAAAGTGC TCCCCAGCAC ATACAGATAG GAAATCGGAA TGAAGTTGTG
    ATCACAGAGA ATAACAACAG CATAACAGAG CAAATAACTG ATGTTGTGAA ACAGCCGGCA
    TTCATCGCAG GAATTGGTGG TGCCTGCTGG GTAATACTCA TGGGATTTAG CATTTGGCTC
    TACTGGCGAA GAAAGAAGAG AAAGGGTCTC AGCAACTACG CTGTTACATT CCAGAGAGGA
    GATGGAGGAC TAATGAGTAA TGGAAGTCGT CCTGGACTTT TAAATGCGAA TGATCCTAGT
    TACCCTTGGT TGGCAGACTC CTGGCCAGCT TCCAGCATGC CGGTGAATAA CAGCAACAAT
    GGACCAAACG ATCTCGTTAA TTTTAGTTGT GGAGATGTGC TGCCAACAGT TCCAGGTCAA
    GGAGAAAAAA CAGCGACTAT GCTTTCTGAT GGAGCCATTT ATAGCAGCAT TGATTTTACT
    ACAAAATCTA GCTATAATTG CCCAGGACAA ATTGCACAAA CCACGCCATA TGCTACTACG
    CAGATACTAC ATTCCAACAG CATTCATGAG CTGGCAGTTG ACTTGCCTGA TCCACAGTGG
    AAATCTTCCA TTCAGCAGAA ATCTGATCTC ACTGGATTAG GCTATTGCCT TACTGACCAA
    GGCAAAGGGA GTAATGGTGG AAAAGACGGA AAAAGGAAGA AAAATAAAAA CAGCTCTAAA
    ATCTCTAAAA ACAATGGATC CAGTTGGGCC AATGTTCCAC TTCCGCCCCC TCCCATCCAA
    CCACTGCCAG GCACTGAAAT AGAACATTAT GGCCTTGAAC ACCAGGAAAC AGGCTATGAC
    ACCAATGGAT GGTGCCCACC TTTGCCAGTC CAGACCTACT TACATCAAGG ATCAGATGAT
    GAACTAGAAG AAGATGAAGA GAGAGTCCCT ACACCCCCTG TACGTGGGGT GGCATCATCA
    CCTGCCATCT CATTTGGACA ACAGTCAACA GCAACCCTCA CACCATCACC ACGTGAAGAA
    ATGCAACCCA TGCTTCAGGC CCACCTGGAT GAATTAACAA GAGCTTACCA GTTTGACATT
    GCAAAGCAAT CTTGGCATTT ACACAGCAAT AACCAGCCCC CATTGGCCCC AGTTCCACCA
    CTTGGATATG TGTCTGGGAC ATTTATTTCT GACTTAGAAA AAGACATTCC AGATCAAGAA
    GAAGATGAAA TTGAAGACGA ATCTATAGAA ATAACAAGGC CCCTCCGAGG TTTGGAACAC
    ACTCCCGGTT CTAGTATGGA TAATCTCGAC AGTTCTGTGA CAGGCAAAAT GAACTCCTTT
    TCTCAGAGAC CCCGGCCTGC CAGTCCCTTT TCTACTGACA GTAACACAAG TTCAGCTGTG
    AATCATAGTC AGAAGTCACG GCCCACTAAG AAACACAAGG GAGGCAGAAT AGATCAGCAA
    CCCATGCTTC GCAGAGAAGG GATTGCAGAT GATTTGCCTC CCCCACCTGA TCCTCCACCT
    TGTCAAAGAC AGCAAATCAT GCCCAGTCAA CGTGGTGGAG GTCCTACAGA AAGAAAAGCC
    AGTTCTCTTG AGAGGCACAC ATCAAATATG AATGATTTAA AAACCTCATT AGACCGGCAT
    GGCAGAACAT CCTTAGAACG GCAGCGACAA ACACAGGAAT GGCTTGGCTC GACAGAAAGG
    CAGAACAAGC AGGCATTTGG ATCTGAAGAG ATACTAATGC CATACAGTAA ACCTAGCTTC
    CCATCTCCAG GAGGGCACAG CTCCTCAGGA ACAGTCTCTT CAAAAGAATC AACTGGACCC
    AGAAAAGGGG AGGTTTTGAG AGGTCACCAA CGTAATGCCA TTGACTTTCT TGATGTAGGA
    TATTTGGGGC CACACAGTCA AGGACAGTTT ACAGGTGAAT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813421.1
    CDS753..5117
    Translation

    Target ORF information:

    RefSeq Version XM_012957967.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis roundabout guidance receptor 2 (robo2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957967.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGGCTTCC TGGTGGTGCC CCTGCTGTGT GGATTGTTAT GCTGTCAGGT GTGGGGATCC 
    CGTCTCCGTC AAGAAGATTT CCCCCCACGG ATAGTTGAGC ATCCATCGGA TGTCATTGTG
    TCAAAGGGAG AGCCAACCAC CTTGAACTGC AAAGCGGAAG GGCGGCCTAC ACCCACCATA
    GAGTGGTACA AAGACTTAGA AAGGGTTGAG ACAGACAAGG ATGACCCCCG CTCACATCGA
    ATGCTACTGC CAAGTGGATC TTTATTTTTT CTGCGCATTA TCCATGGGCG CAAGTTTAAG
    CCAGACGAAG GAAGTTACGT CTGTGTGGCA AGGAACTATC TCGGGGAGGC TATGAGTCGA
    AATGCATCTC TGGAAGTGGC ATTGCTCCGT GATGACTTCC GGCTCAACCC TACAGATGTT
    GTTGTGGCAG CTGGGGAACC TGCAATTTTG GAGTGTCAAC CCCCCAGAGG ACATCCGGAA
    CCAACTATAT ACTGGAAAAA GGACAAAGTT AGAATTGATG ATAAAGATGA AAGAATTAGT
    ATTCGAGGTG GAAAGCTGAT GCTCTCCAAT ACAAGGAAAA GTGATGCAGG CATGTACACT
    TGTGTTGGGA CCAACATGGT TGGAGAAAGA GACAGTGACC CAGCAGAGCT AACAGTCTTT
    GAACGTCCCA CATTCCTCAG AAGGCCAATT AATCAAGTTG TTTTAGAGGA TGAAGCTGTA
    GAATTTCGAT GTCAGGTCCA GGGGGACCCA CAGCCAACTG TCCGGTGGAA AAAGGATGAT
    GTAGATTTGC CAAGAGGAAG ATACGACATC AAGGATGACT ATACTTTGAG AATCAAGAAG
    GCTTCCAGCA GCGACGAAGG GACCTTTGCT TGTATTTCTG AAAACCGGGT TGGTAAAATA
    GAAGCTTCTG CCACACTTAC AGTCAGAGCT CCTCCCCAGT TTGTGATCAG ACCAAGGGAT
    CAGATTGTAG CACAAGGCCG GACAGTAACA TTTGGATGTG AAGCTAAAGG AAATCCTCAA
    CCAGCTGTTT TTTGGCAAAA AGAAGGAAGC CAGAATTTAC TTTTTCCAAA TCAGCCTCTT
    CAGCCCAACA GCAGGTACTC AGTGTCACCA ACTGGAGATT TAACCATCAC CAGCATTCAG
    CGCTCAGACG CAGGCTACTA CATTTGTCAG GCACTGACTG TTGCCGGAAG CATTCTGGCC
    AAGGCCCAGC TGGAAGTGAC TGATGTTTTG ACAGATAGAC CACCGCCAAT AATTCTACAA
    GGTCCAGCTA ATCAAACTCT TGCCGTGGAT GGAACAGCAG TGCTGAAATG TAAAACTACT
    GGGGACCCAA TGCCAGTCAT AAGTTGGCTA AAAGAGGGAT TTAAGGGATT CATAGGGAGA
    GATGCAAGGT TATCCATTCT AGACCAAGGG ACATTACAAA TTAAAGGTTT AAAGGTTACT
    GATTCTGGCA CCTATACTTG TGTGGCTACT AGCTCTAGTG GTGAGACAAC ATGGAGCGCT
    GTTCTGGAAG TGACAGAATC TGGTGGTGCC ATAATTAGCA AGAACTATGA TCTCAATGAC
    CTTCCTGGAT CTCCATCAAA ACCTCAGGTT ACAGATGTCA GTAAGAACAG TGTTACCCTA
    TCATGGCAAC CAGGATCACC AGGGATTCTG CCTGTGACAT CCTACATCAT TGAGGCTTTC
    AGTCAATCTG TGAGCAATAG TTGGCAAACT GTGGCCAATC ACGTGAAGAC AACGCTTTAC
    ACTGTTAAAG GACTGCGCCC AAACACCATT TATCTCTTTA TGGTGAGAGC AATCAATACT
    GAAGGACTGA GTGACCCAAG TCCAATATCT GATCCAGTGC GTACACAAGA TATAAGTCCA
    CCTGTTCAAG GAGTAGATCA CCGACAAGTA CAGAAAGAAC TTGGGGATGT GATTGTACGG
    CTCCATAATC CTGTTGTTCT GACACCAACA ACAATCCAGG TCACCTGGAT GGTCGACCGT
    CAATCCCAGT TTATTCAGGG ATACAGGGTA ATGTACCGTC AGACTTCTGG TCTGCAGACT
    CCATCTGCGT GGCAGAGCTT AGAGGTCAAA GTGCCTGCAG AGCGTAGCGC AGTGCTAACC
    AACCTGAAAA AAGGAATTAC TTATGAGATT AAGGTTCGAC CTTATTTTAA TGAATTTCAA
    GGAATGGATA GCGAGTCAAA GTCAGCACGA ACAACTGAAG AAGCCCCAAG TGCTCCTCCT
    CAGTCTGTTA CTGTTATGAC AGTGGGAAAT AACAACAGCA CCAGCATCAG CATCTCATGG
    GACCCCCCTC CATCTGACCA CCAGAATGGA ATTATCCAGG ACTACAGGAT CTGGTGCCTG
    GGTAATGAGA CACGTTTCCA CATCAACAAG ACAGTGGATG CTGCTATTCG TTCCGTGGTT
    ATCGGTGGGC TATTCCCTGG AATTCAGTAC CGCGTTGAAG TCGCAGCCAG TACAAGCGCA
    GGCACTGGGG TAAAAAGTGC TCCCCAGCAC ATACAGATAG GAAATCGGAA TGAAGTTGTG
    ATCACAGAGA ATAACAACAG CATAACAGAG CAAATAACTG ATGTTGTGAA ACAGCCGGCA
    TTCATCGCAG GAATTGGTGG TGCCTGCTGG GTAATACTCA TGGGATTTAG CATTTGGCTC
    TACTGGCGAA GAAAGAAGAG AAAGGGTCTC AGCAACTACG CTGTTACATT CCAGAGAGGA
    GATGGAGGAC TAATGAGTAA TGGAAGTCGT CCTGGACTTT TAAATGCGAA TGATCCTAGT
    TACCCTTGGT TGGCAGACTC CTGGCCAGCT TCCAGCATGC CGGTGAATAA CAGCAACAAT
    GGACCAAACG ATCTCGTTAA TTTTAGTTGT GGAGATGTGC TGCCAACAGT TCCAGGTCAA
    GGAGAAAAAA CAGCGACTAT GCTTTCTGAT GGAGCCATTT ATAGCAGCAT TGATTTTACT
    ACAAAATCTA GCTATAATTG CCCAGGACAA ATTGCACAAA CCACGCCATA TGCTACTACG
    CAGATACTAC ATTCCAACAG CATTCATGAG CTGGCAGTTG ACTTGCCTGA TCCACAGTGG
    AAATCTTCCA TTCAGCAGAA ATCTGATCTC ACTGGATTAG GCTATTGCCT TACTGACCAA
    GGCAAAGGGA GTAATGGTGG AAAAGACGGA AAAAGGAAGA AAAATAAAAA CAGCTCTAAA
    ATCTCTAAAA ACAATGGATC CAGTTGGGCC AATGTTCCAC TTCCGCCCCC TCCCATCCAA
    CCACTGCCAG GCACTGAAAT AGAACATTAT GGCCTTGAAC ACCAGGAAAC AGGCTATGAC
    ACCAATGGAT GGTGCCCACC TTTGCCAGTC CAGACCTACT TACATCAAGG ATCAGATGAT
    GAACTAGAAG AAGATGAAGA GAGAGTCCCT ACACCCCCTG TACGTGGGGT GGCATCATCA
    CCTGCCATCT CATTTGGACA ACAGTCAACA GCAACCCTCA CACCATCACC ACGTGAAGAA
    ATGCAACCCA TGCTTCAGGC CCACCTGGAT GAATTAACAA GAGCTTACCA GTTTGACATT
    GCAAAGCAAT CTTGGCATTT ACACAGCAAT AACCAGCCCC CATTGGCCCC AGTTCCACCA
    CTTGGATATG TGTCTGGGAC ATTTATTTCT GACTTAGAAA AAGACATTCC AGATCAAGAA
    GAAGATGAAA TTGAAGACGA ATCTATAGAA ATAACAAGGC CCCTCCGAGG TTTGGAACAC
    ACTCCCGGTT CTAGTATGGA TAATCTCGAC AGTTCTGTGA CAGGCAAAAT GAACTCCTTT
    TCTCAGAGAC CCCGGCCTGC CAGTCCCTTT TCTACTGACA GTAACACAAG TTCAGCTGTG
    AATCATAGTC AGAAGTCACG GCCCACTAAG AAACACAAGG GAGGCAGAAT AGATCAGCAA
    CCCATGCTTC GCAGAGAAGG GATTGCAGAT GATTTGCCTC CCCCACCTGA TCCTCCACCT
    TGTCAAAGAC AGCAAATCAT GCCCAGTCAA CGTGGTGGAG GTCCTACAGA AAGAAAAGCC
    AGTTCTCTTG AGAGGCACAC ATCAAATATG AATGATTTAA AAACCTCATT AGACCGGCAT
    GGCAGAACAT CCTTAGAACG GCAGCGACAA ACACAGGAAT GGCTTGGCTC GACAGAAAGG
    CAGAACAAGC AGGCATTTGG ATCTGAAGAG ATACTAATGC CATACAGTAA ACCTAGCTTC
    CCATCTCCAG GAGGGCACAG CTCCTCAGGA ACAGTCTCTT CAAAAGAATC AACTGGACCC
    AGAAAAGGGG AGGTTTTGAG AGGTCACCAA CGTAATGCCA TTGACTTTCT TGATGTAGGA
    TATTTGGGGC CACACAGTCA AGGACAGTTT ACAGGTGAAT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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