golga4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	golga4
Entrez Gene ID	100495226
Full Name	golgin A4
Synonyms	gcp2,golg,p230
General protein information	Preferred Names golgin A4 Names golgin subfamily A member 4
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018094817.1	XP_017950306.1	golgin subfamily A member 4 isoform X2
XM_018094817.2	XP_017950306.1	golgin subfamily A member 4 isoform X2
XM_018094816.1	XP_017950305.1	golgin subfamily A member 4 isoform X1
XM_018094816.2	XP_017950305.1	golgin subfamily A member 4 isoform X1
XM_018094818.2	XP_017950307.1	golgin subfamily A member 4 isoform X3
XM_018094818.1	XP_017950307.1	golgin subfamily A member 4 isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GOLGA4	XP_003309745.1
Mus musculus (house mouse)	Golga4	NP_061218.2
Homo sapiens (human)	GOLGA4	NP_001166184.1
Xenopus tropicalis (tropical clawed frog)	golga4	XP_004915409.1
Rattus norvegicus (Norway rat)	Golga4	NP_001178009.1
Gallus gallus (chicken)	GOLGA4	XP_418856.4
Canis lupus familiaris (dog)	GOLGA4	XP_005634302.1
Bos taurus (cattle)	GOLGA4	NP_001179054.1
Danio rerio (zebrafish)	golga4	XP_001921298.3
	GOLGA4	XP_001090210.2

Gene Ontology

The following golga4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the golga4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa45016	XM_018094816.2 Latest version!	Xenopus tropicalis golgin A4 (golga4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa45017	XM_018094817.2 Latest version!	Xenopus tropicalis golgin A4 (golga4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa45018	XM_018094818.2 Latest version!	Xenopus tropicalis golgin A4 (golga4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OXa45017	XM_018094817.1	Xenopus tropicalis golgin A4 (golga4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OXa45016	XM_018094816.1	Xenopus tropicalis golgin A4 (golga4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OXa45018	XM_018094818.1	Xenopus tropicalis golgin A4 (golga4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

golga4 ( XM_018094816.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017950305.1 Xenopus tropicalis golgin A4 (golga4), transcript variant X1, mRNA.

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CloneID	OXa45016
Clone ID Related Accession (Same CDS sequence)	XM_018094816.1 , XM_018094816.2
Accession Version	XM_018094816.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	golgin subfamily A member 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAAGA AACTGAAACA GAAGATCAGC GAAGAGCAGT CCCCTTCCAA AAGCCCCCTT 
GCTCCACAGC AGCAGGGTCG TCCAAGAAGC ACATCAACAG GCAACAGAAG TCGGACTTCG 
TCAGTAACAG AACAGCAGGA TGACAGCCTG GCAACAGCAG ATAGAGAGAA CGGATCATTG 
TCGGACAATA TTAATGGAAA TGATCCCACT TCTTCTCAGA GGAATGAACC TCAGTCTTTT 
GCACATAAGC TACAAATGCG GGTTCCTTCA ATGGAATCAC TGTTTCGAAG CCCCATTAAA 
GAATCACTTT TTCCTTCTTC AACTAAAGAA AATCTTTTGC GGAGTAGTTC ACGGGATTCC 
CTGAATAAAA TTGATATGGA GAATCCAAGT ACTGTATTTG ATCCCCCATC CGATATTGAA 
AGTGAAGCTG AGGATTCTGT GGGAAGTATA GAAGGACTTA ACAAAGAACA GCTGTTTAAT 
CGACTACATA GAATGGATAA AAGTTTAGGA AACTACAGAG GAAAATATTC TGAGCTTGTA 
ACAGCCTACA GAACAATGCA GAGAGACAAA GAGAAACTAC AGTCCATCTT GAGTCAGAGC 
CAGGACAAAG CACTAAGGAG AATTGGAGAG CTGCGAGAGG AGCTTCAAAT GGATCAACAG 
GCTAAAAAGC ATCTACAGGA AGAGTTTGAT GCATCTCTGG AAGAGAAAGA TCAACTTATC 
AGTGTTCTGC AAACACAGGT TAGTTTACTT AAAAACAGAC TTCAGAATGG ACAAAGCAGT 
ACTGAATTAC CAGAAAGCAC AGCCTCGCCA GAACTTGACT CTGAGAGTTC CTTGCAAGAG 
GTGGATACAG ATAACCGTTC CACCCCTGAT GATGGGAATG CTGCAAACAC TTTAAATGCT 
CTCCAGGTTA GAGTAAAACG TCAAGAGAAC CTTTTGCAAA GGTGTAAGAA ATCAATCAAG 
TCGCACAAGG AACAGTGTGC TCACCTCAGT AGTGAAAATG AAGCTCTTCA AAACCAACTA 
GATGAAAGAC TCCAGGAACT TGAGAAAATT AAAGAACTTC ACACTAGTGA GAAGAGCAAG 
CTGATTACCC AGCTTAGAGA TGCCAAGAAT TCAATTGAAC AGCTGGAACA AGATAAGGGT 
ATGATGATTG CAGAAACAAA ACGGCAGATG CATGAGACAT TAGAACTGAA GGAAGAAGAG 
GTTTCTCAGC TTCGGTCAAG GGTCAAGAAA TTAAGTTCAC ATTGTGAGGA ACTGCAGCAA 
CAGAAAGAAA AGTTAGAGAA AGCTGCATTT GAAGATGTAG AAAAAATATC TTCACAGAAA 
GCTGAGGATG CTTTTGAAAA GCTGCAGATG GAAGAGCAAA TTAAAGCTAT AGAAAAGGCC 
AGTGAGGAGG AGCGTGTCAA TCTTCAGAAG GAATTAAGTC GTGTGAAGCA AGAGGTGGTG 
GACATTATGA AGAAATCCTG TGATAGTCGT ATTAGTGAGA TTGAGAAAGT TCATGCAGAA 
GCCTTGCTGA ATAAGGAACA GGAGTATAAT GAACAGATTC GCCTGAAGGA ACAAGAATTC 
CAGGATCAAA TGAATGCTGT CAAAAAGAAT GAGGATGAGA CATTAAATTT GCTACAAGAT 
AAAGAAAAGA GTTTAATGGC ACTGGAAGAG CTCGAGCGAC AGAAAAAGAC TATTGAGTCC 
AGAGCAGCAG AACAACTTAA AGAAATGCAA CAAGAAGTTG AAAATTGCAG AATGAGGATT 
CTTGACCTTG AGAGTTCTGT TGCTGAAGAA AGTAAGTCCC AAGATTTTGT AAGCCTGATT 
GAAACAGAGA GAAATAATCA CAATGCAGAA GTAGCTACCA TCAAAGAAAT GCATAAGCAA 
GATCTTGATG TCCTTGTACA GGATCAAGAA AGGATATGGT CAGAAAAGCT TGAGCTGCTT 
AAGAAAGAGA ATCATACTAA GATTATTGAA CTTAAAGAGA AGTATCAGCG AGATTATGAT 
TCTCACTTGA AAGAGAAAGA GCATAATTTC CAGGAGCATA TTGAGCATAT GAATGAAAAA 
ACATTAGAAA AACTAGATGT AAAACAAACT GAATTGGAAA CCCTCTCAGC TGAACTGTCT 
GAGGCTATAA AGGCACGGCA AGAGATTGAG CAGAAACTTA CAGACACAGA AAATGAGATT 
AGCAAAATAA AACTAGAGTA TGAAGCCCAG ATAAAAGAAG AAAGAGAAAA ACACAGTGAA 
GAAATTGGTT CCATTGAAAG AGAGAAGGAA ATGCATACTC AGGGTGTTGA GAAAGACTTG 
AAGGAAAATA TTAACAACCT AAAACTACTT CAAGATAGTA AAGAATGTGA GTTGGAAGAA 
TTCAGAAAGG AAAATCAAAA GCTAAAAGAA GCTGCTGAAA AGGCAGAAGC AGACCATAAA 
GAAGCATTTG CTCTATTGGA AGCAGCCAGG CATTCTCATG AGATAAATAC ACATGAGAAA 
GAACAGGCTT ATGAGCTAAA ATTGAAGGAG CTTCAGGAGA TGCTAAAAGG TATGACGCAG 
GAGCGAAACG ATCTTAAAGA GGTGACAGAA AAAGCAGAAA CACAGTTAAA GAATATGCAG 
ATCGAGCTGG ATTCTAACAA GGCCCAAGTA CAAAGCCTTA CAAAGCAAAT TCATGAGGAA 
AGTATGAATA TGGCAGATAA ACTTAACACT TTGGTACTAG AGTCTGAAAC TCAGGTAAAA 
GGGTTGAAGG AACAAATAGA AGAGATAAGC AGAATAGCTT CAGAGCGTGA ACATGAACTT 
AAAAAAGTAA AAGAAGATCA TATTCAAATT ATTTTAGACC TAAACAATAC TATATCTGAA 
AAAGAGAAGA ATATACTTTC TTTGCAAGAA GATTACAAAG TAAAACACAA AAATCTGGAT 
GCTAAAATGG AAAAATTAAA GCAGAAGTTT AAAGAGCTGC AAGAATCATC TAAAAAGAAA 
TATTCAGAGT TGGAAAGCAA ACTTAAGAAA GAGATCGAGA AGAAGCAGAA CGAGTTGATT 
AACAAAGAAA GGGAGTTTAA TGAAAAAATA CATGAGATGG CCCAAGCAAG TTCAACTGGA 
ATTAATCATG CAATGGCTCA GCTTGAACTG AATCATAAAG AGCAGATTCA GAATATGACA 
GACAATCATC AGAGAGAATT ACAGGAAACA ACAGAAGCTT GTGAAAAGAA ATTAAGCCAA 
CTTGCTGAAG ACCTTAGAGA GAAGCACGAA GAAGAACTTC AGGAGAAAGA TCAGGTCATA 
GCAGAATCAA GAAAACAGTT TAATATCAAG AATAAAGAAA AAGAAGATGC AGACAAAGAG 
ATATCAGACC TCAGGGAACT GAAGACCCAA ATGGATGCCA CGTTTAATGA TTTGCAAGAA 
CAGCTGTGTC AGTCAGCAGC TCAAGTTTTA CATTTGACAC AGAGTGAAAA TGAATTCAAG 
ACAAATTGTA CTCGGCTAAC AACGGAGAAG CAAATTCTTC AACAACAGCT TGATGAACTG 
AAAATATTAG CAGAGGAAGA TAAAAGCAAG AATGATGAAA TAGTTAACAA GGTGAGACTT 
GCTGAAGAAA AGTATCAAGA CTTGGAGACC CTGCACAATA AAGTGAGAGC AGACTTTGCT 
AAGGAACTGC AGGATAAACT TGTTGAAAAG GAAAATGAGT TTAAACACCT TTTGGTTAAA 
CAAGAAAACC AGTTGATCTT GTGCTGCAAA AATGCTGAAG ATTTATTTGA AACACTTGCA 
AATGGACTCA CTGAAAACCA CAAATATAAG TTGAATGGAT TGATTGTTAA AATAGCAAAT 
CTTGAAAGCT GCATGCATAA ACTAAAAGAG GTCACTTTAA AACAGCGTAG TAGAATATAT 
GAACAAGAAA AACAACTTGA GCAGTTTTCA AACGAACTTC TTTCAGTAAA CCAATCTCTT 
AATCAAGCAA CTCAGGAGAA AGAATTTGCT ACTTTGTCGC TAAAGAATGA GATTGAAGCT 
CTTAATTTTC AAAATGAGAT GTTAAAAAAA GAAGGAGGAA GTCAACAGCA GGTTGCAGAT 
GAGAAAGAAT CTTGCATTAC TCAGCTGAAA AAAGAACTTT CAGAGAATAT AAATATTGTG 
ACTTCCTTGA CAGAAGTGGT AAAACAGAAA GAATCTGAGG TATCTTCTCT TTCTGCCTTA 
ATTAATGAAC TTAAACTGAA ACATGAGAAT TGTGTAGATT TAACAGAAAA AGAAAATGCA 
ATTGCTTTTC TCACTGCACA GCAGAAACAG AGTCAGCAAG AGCTACAGAA TCAAATTCAG 
GATCTAATGG CAAAGATTGA GGTTCTGAAT AAAGAAAAAT CTGCACAAAT AGATGAGGCT 
GCTAAACTTA AAAATAAGTT GGATGAATGG AAGAAAAAGG CAGAACTTAA ATTTGTGCAG 
AACCACAACA CTATTAAAGA GCTAAAAGAG AAAGTTGAAT CAGTCAATAA GCAAATTGTT 
GAAAAAGATG AAAAACTTAG GACAGTGACA GAAGAATTTG AATGTCATCG TAGCAATATC 
CAAGAAACAC AACTCATGAG TGAAAGCAAA GAATTTGAAT TAACTACCCA GTTGGAAGGC 
CAGAAATCTA AAATTTCGGA ATTGGAGGAC CAGTGTTCGA AGCTGATGTC TGAAAAGAAA 
AGTTTGATGG ATCAAATCGA AATTCAAACT ATTCAGCAGA GTGAAGATAA GAAAGCACTG 
GTTGAGCAAC TCCAGCATAT TCAGTATGCT TCTGACAATA ACTCCAAGGA AGTGCAAAAT 
AAAGTTATAA CACTTGAAAA AGAAATTGAT GCTTTAAAGG AGAAACTTGA TGCAGAGCAA 
ACAAAATGGC AGCTGGAGAA AGCAGAGTGC TTTCAGCTGA AAGATAAGGA ATTAAAAATA 
TTGGAAGAGA AACTAACTGC AGAAAGCACA AATAAACTTT CAGAGCTCAA GAAAAAAGCT 
GAACAGAAAA TCGCTTCTCT TAAGAAACAA CTTATGTCTC AGATAGATGA GAAGGAGCAG 
TCCAGGAAAG CGCTGGAAAG CCAGCTAAAT GTCTTAAAAG ATAATATGCA GAAAGAGAGC 
CAGGAAGTGC AGGAAGGATT GCAACAAAAA GTTGAAAATC AAGACAAAAA ATATGCATTG 
CTACTGGAGC AGAAGAAAAA TACAGAAGCA GATTTAGCTA ATATTAGAAG TGAACTAAAA 
TTAAAGGAAC TAAGAGTTAA AGAAATGGAA GATATTCTTA AAGATGTCCA GCAAAAACAT 
TCTGAAAGAG AGCTGACCGA CAGTGAGAAA GAACAAGCAC TTTTAAAAGA ACTAGAGTCA 
CAGATGCTGA CAAATAATCA GCTGGTTTCT ACTCATGAAA AAGCTTTAAG AGCCCTGCAA 
GATGACCTCA ATGTCAAGGA TGCAGAGATA AAGACCTTTA AAGAGGAAAT AGATGAGAAA 
TCCAAATCCT TAAATGAACT CCAAGTGCTT TTTGAGGAGA CTCAGTCTCA AGAGAGCGCA 
ATGAGAATAA AACTTGAGGA AATGGAGAGT GAGAAGCTAA AATTCAAGAA TGAAATAACT 
AAGCTACAGA AAGATATGCG CTCTTTACGT AAGGAACACA GCCAAGAACT AGATTTGCTT 
AAAAAGGAGA TAACCGAGGA AGCCGAACAA AGAATCAAAT ACGAACAGGA AGACTCGGAA 
CTTAAGCATA ATTCTAAATT AAAGCAACTC ATGAGAGAAT TCAATACCCA GATGGCTCAA 
AAGGAGCGTG AGGTTGAAAC AGCTGTTCAG GAGACTATTG CTAAAGCTCA GGAGGTTGAG 
GCTGAATTAA TGCGATCACA ACAGATAGAA GCAGTCCATC TTCATAAAAA GATTGCTGAG 
AAAGAAGATG ACCTAAAGAG AACTGTAAAA AGATATGAAG AACTTTTAGA GGCCCGAGAA 
GAGGAAATGA CAGCAAAAGT GACAGAGTTA CAAGAGCAAC TTGAAAGATT ACAGGAACAA 
TTAACTCAGA AGGAAATGCA AAATGAAGAA GAAGAAAATG TGGAGGAGGC AACATCTCCT 
GAGGTTTTTC AGGCGCAGCT GGCACAGAAA ACATCGCAGG TCAATGAATA CAAACTGAAG 
GAGCAGGAAC TTAAGGAGAA GGTTCACATC CTAGAAGACC AGGTGAAAAA CTTTACTCGT 
GGTGTATTTT ATACTCCTAT TGGCACACCG TATAAAGAAG TTAATAATCA ACATGCAGGG 
GTTTCTGTTT TTGGCGAACC CACTGAATTT GAGTACTTAA GAAAGGTGAT GTTTGAGTAC 
ATGATGGGGC GTGAAACAAA GACAATGGCA AAGGTAATAA CAACTGTGCT GAGGTTCCCA 
ACAGATCAAG CTCAACAAAT TTTGCAACGA GAAGAAACCC GACCACTGTC TTGGCTTCTA 
TCTTCCTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017950305.1
CDS	681..7052
Translation	MFKKLKQKISEEQSPSKSPLAPQQQGRPRSTSTGNRSRTSSVTEQQDDSLATADRENGSLSDNINGNDPTSSQRNEPQSFAHKLQMRVPSMESLFRSPIKESLFPSSTKENLLRSSSRDSLNKIDMENPSTVFDPPSDIESEAEDSVGSIEGLNKEQLFNRLHRMDKSLGNYRGKYSELVTAYRTMQRDKEKLQSILSQSQDKALRRIGELREELQMDQQAKKHLQEEFDASLEEKDQLISVLQTQVSLLKNRLQNGQSSTELPESTASPELDSESSLQEVDTDNRSTPDDGNAANTLNALQVRVKRQENLLQRCKKSIKSHKEQCAHLSSENEALQNQLDERLQELEKIKELHTSEKSKLITQLRDAKNSIEQLEQDKGMMIAETKRQMHETLELKEEEVSQLRSRVKKLSSHCEELQQQKEKLEKAAFEDVEKISSQKAEDAFEKLQMEEQIKAIEKASEEERVNLQKELSRVKQEVVDIMKKSCDSRISEIEKVHAEALLNKEQEYNEQIRLKEQEFQDQMNAVKKNEDETLNLLQDKEKSLMALEELERQKKTIESRAAEQLKEMQQEVENCRMRILDLESSVAEESKSQDFVSLIETERNNHNAEVATIKEMHKQDLDVLVQDQERIWSEKLELLKKENHTKIIELKEKYQRDYDSHLKEKEHNFQEHIEHMNEKTLEKLDVKQTELETLSAELSEAIKARQEIEQKLTDTENEISKIKLEYEAQIKEEREKHSEEIGSIEREKEMHTQGVEKDLKENINNLKLLQDSKECELEEFRKENQKLKEAAEKAEADHKEAFALLEAARHSHEINTHEKEQAYELKLKELQEMLKGMTQERNDLKEVTEKAETQLKNMQIELDSNKAQVQSLTKQIHEESMNMADKLNTLVLESETQVKGLKEQIEEISRIASEREHELKKVKEDHIQIILDLNNTISEKEKNILSLQEDYKVKHKNLDAKMEKLKQKFKELQESSKKKYSELESKLKKEIEKKQNELINKEREFNEKIHEMAQASSTGINHAMAQLELNHKEQIQNMTDNHQRELQETTEACEKKLSQLAEDLREKHEEELQEKDQVIAESRKQFNIKNKEKEDADKEISDLRELKTQMDATFNDLQEQLCQSAAQVLHLTQSENEFKTNCTRLTTEKQILQQQLDELKILAEEDKSKNDEIVNKVRLAEEKYQDLETLHNKVRADFAKELQDKLVEKENEFKHLLVKQENQLILCCKNAEDLFETLANGLTENHKYKLNGLIVKIANLESCMHKLKEVTLKQRSRIYEQEKQLEQFSNELLSVNQSLNQATQEKEFATLSLKNEIEALNFQNEMLKKEGGSQQQVADEKESCITQLKKELSENINIVTSLTEVVKQKESEVSSLSALINELKLKHENCVDLTEKENAIAFLTAQQKQSQQELQNQIQDLMAKIEVLNKEKSAQIDEAAKLKNKLDEWKKKAELKFVQNHNTIKELKEKVESVNKQIVEKDEKLRTVTEEFECHRSNIQETQLMSESKEFELTTQLEGQKSKISELEDQCSKLMSEKKSLMDQIEIQTIQQSEDKKALVEQLQHIQYASDNNSKEVQNKVITLEKEIDALKEKLDAEQTKWQLEKAECFQLKDKELKILEEKLTAESTNKLSELKKKAEQKIASLKKQLMSQIDEKEQSRKALESQLNVLKDNMQKESQEVQEGLQQKVENQDKKYALLLEQKKNTEADLANIRSELKLKELRVKEMEDILKDVQQKHSERELTDSEKEQALLKELESQMLTNNQLVSTHEKALRALQDDLNVKDAEIKTFKEEIDEKSKSLNELQVLFEETQSQESAMRIKLEEMESEKLKFKNEITKLQKDMRSLRKEHSQELDLLKKEITEEAEQRIKYEQEDSELKHNSKLKQLMREFNTQMAQKEREVETAVQETIAKAQEVEAELMRSQQIEAVHLHKKIAEKEDDLKRTVKRYEELLEAREEEMTAKVTELQEQLERLQEQLTQKEMQNEEEENVEEATSPEVFQAQLAQKTSQVNEYKLKEQELKEKVHILEDQVKNFTRGVFYTPIGTPYKEVNNQHAGVSVFGEPTEFEYLRKVMFEYMMGRETKTMAKVITTVLRFPTDQAQQILQREETRPLSWLLSSS

Target ORF information:

RefSeq Version	XM_018094816.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis golgin A4 (golga4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094816.1

ORF Insert Sequence:

ATGTTTAAGA AACTGAAACA GAAGATCAGC GAAGAGCAGT CCCCTTCCAA AAGCCCCCTT 
GCTCCACAGC AGCAGGGTCG TCCAAGAAGC ACATCAACAG GCAACAGAAG TCGGACTTCG 
TCAGTAACAG AACAGCAGGA TGACAGCCTG GCAACAGCAG ATAGAGAGAA CGGATCATTG 
TCGGACAATA TTAATGGAAA TGATCCCACT TCTTCTCAGA GGAATGAACC TCAGTCTTTT 
GCACATAAGC TACAAATGCG GGTTCCTTCA ATGGAATCAC TGTTTCGAAG CCCCATTAAA 
GAATCACTTT TTCCTTCTTC AACTAAAGAA AATCTTTTGC GGAGTAGTTC ACGGGATTCC 
CTGAATAAAA TTGATATGGA GAATCCAAGT ACTGTATTTG ATCCCCCATC CGATATTGAA 
AGTGAAGCTG AGGATTCTGT GGGAAGTATA GAAGGACTTA ACAAAGAACA GCTGTTTAAT 
CGACTACATA GAATGGATAA AAGTTTAGGA AACTACAGAG GAAAATATTC TGAGCTTGTA 
ACAGCCTACA GAACAATGCA GAGAGACAAA GAGAAACTAC AGTCCATCTT GAGTCAGAGC 
CAGGACAAAG CACTAAGGAG AATTGGAGAG CTGCGAGAGG AGCTTCAAAT GGATCAACAG 
GCTAAAAAGC ATCTACAGGA AGAGTTTGAT GCATCTCTGG AAGAGAAAGA TCAACTTATC 
AGTGTTCTGC AAACACAGGT TAGTTTACTT AAAAACAGAC TTCAGAATGG ACAAAGCAGT 
ACTGAATTAC CAGAAAGCAC AGCCTCGCCA GAACTTGACT CTGAGAGTTC CTTGCAAGAG 
GTGGATACAG ATAACCGTTC CACCCCTGAT GATGGGAATG CTGCAAACAC TTTAAATGCT 
CTCCAGGTTA GAGTAAAACG TCAAGAGAAC CTTTTGCAAA GGTGTAAGAA ATCAATCAAG 
TCGCACAAGG AACAGTGTGC TCACCTCAGT AGTGAAAATG AAGCTCTTCA AAACCAACTA 
GATGAAAGAC TCCAGGAACT TGAGAAAATT AAAGAACTTC ACACTAGTGA GAAGAGCAAG 
CTGATTACCC AGCTTAGAGA TGCCAAGAAT TCAATTGAAC AGCTGGAACA AGATAAGGGT 
ATGATGATTG CAGAAACAAA ACGGCAGATG CATGAGACAT TAGAACTGAA GGAAGAAGAG 
GTTTCTCAGC TTCGGTCAAG GGTCAAGAAA TTAAGTTCAC ATTGTGAGGA ACTGCAGCAA 
CAGAAAGAAA AGTTAGAGAA AGCTGCATTT GAAGATGTAG AAAAAATATC TTCACAGAAA 
GCTGAGGATG CTTTTGAAAA GCTGCAGATG GAAGAGCAAA TTAAAGCTAT AGAAAAGGCC 
AGTGAGGAGG AGCGTGTCAA TCTTCAGAAG GAATTAAGTC GTGTGAAGCA AGAGGTGGTG 
GACATTATGA AGAAATCCTG TGATAGTCGT ATTAGTGAGA TTGAGAAAGT TCATGCAGAA 
GCCTTGCTGA ATAAGGAACA GGAGTATAAT GAACAGATTC GCCTGAAGGA ACAAGAATTC 
CAGGATCAAA TGAATGCTGT CAAAAAGAAT GAGGATGAGA CATTAAATTT GCTACAAGAT 
AAAGAAAAGA GTTTAATGGC ACTGGAAGAG CTCGAGCGAC AGAAAAAGAC TATTGAGTCC 
AGAGCAGCAG AACAACTTAA AGAAATGCAA CAAGAAGTTG AAAATTGCAG AATGAGGATT 
CTTGACCTTG AGAGTTCTGT TGCTGAAGAA AGTAAGTCCC AAGATTTTGT AAGCCTGATT 
GAAACAGAGA GAAATAATCA CAATGCAGAA GTAGCTACCA TCAAAGAAAT GCATAAGCAA 
GATCTTGATG TCCTTGTACA GGATCAAGAA AGGATATGGT CAGAAAAGCT TGAGCTGCTT 
AAGAAAGAGA ATCATACTAA GATTATTGAA CTTAAAGAGA AGTATCAGCG AGATTATGAT 
TCTCACTTGA AAGAGAAAGA GCATAATTTC CAGGAGCATA TTGAGCATAT GAATGAAAAA 
ACATTAGAAA AACTAGATGT AAAACAAACT GAATTGGAAA CCCTCTCAGC TGAACTGTCT 
GAGGCTATAA AGGCACGGCA AGAGATTGAG CAGAAACTTA CAGACACAGA AAATGAGATT 
AGCAAAATAA AACTAGAGTA TGAAGCCCAG ATAAAAGAAG AAAGAGAAAA ACACAGTGAA 
GAAATTGGTT CCATTGAAAG AGAGAAGGAA ATGCATACTC AGGGTGTTGA GAAAGACTTG 
AAGGAAAATA TTAACAACCT AAAACTACTT CAAGATAGTA AAGAATGTGA GTTGGAAGAA 
TTCAGAAAGG AAAATCAAAA GCTAAAAGAA GCTGCTGAAA AGGCAGAAGC AGACCATAAA 
GAAGCATTTG CTCTATTGGA AGCAGCCAGG CATTCTCATG AGATAAATAC ACATGAGAAA 
GAACAGGCTT ATGAGCTAAA ATTGAAGGAG CTTCAGGAGA TGCTAAAAGG TATGACGCAG 
GAGCGAAACG ATCTTAAAGA GGTGACAGAA AAAGCAGAAA CACAGTTAAA GAATATGCAG 
ATCGAGCTGG ATTCTAACAA GGCCCAAGTA CAAAGCCTTA CAAAGCAAAT TCATGAGGAA 
AGTATGAATA TGGCAGATAA ACTTAACACT TTGGTACTAG AGTCTGAAAC TCAGGTAAAA 
GGGTTGAAGG AACAAATAGA AGAGATAAGC AGAATAGCTT CAGAGCGTGA ACATGAACTT 
AAAAAAGTAA AAGAAGATCA TATTCAAATT ATTTTAGACC TAAACAATAC TATATCTGAA 
AAAGAGAAGA ATATACTTTC TTTGCAAGAA GATTACAAAG TAAAACACAA AAATCTGGAT 
GCTAAAATGG AAAAATTAAA GCAGAAGTTT AAAGAGCTGC AAGAATCATC TAAAAAGAAA 
TATTCAGAGT TGGAAAGCAA ACTTAAGAAA GAGATCGAGA AGAAGCAGAA CGAGTTGATT 
AACAAAGAAA GGGAGTTTAA TGAAAAAATA CATGAGATGG CCCAAGCAAG TTCAACTGGA 
ATTAATCATG CAATGGCTCA GCTTGAACTG AATCATAAAG AGCAGATTCA GAATATGACA 
GACAATCATC AGAGAGAATT ACAGGAAACA ACAGAAGCTT GTGAAAAGAA ATTAAGCCAA 
CTTGCTGAAG ACCTTAGAGA GAAGCACGAA GAAGAACTTC AGGAGAAAGA TCAGGTCATA 
GCAGAATCAA GAAAACAGTT TAATATCAAG AATAAAGAAA AAGAAGATGC AGACAAAGAG 
ATATCAGACC TCAGGGAACT GAAGACCCAA ATGGATGCCA CGTTTAATGA TTTGCAAGAA 
CAGCTGTGTC AGTCAGCAGC TCAAGTTTTA CATTTGACAC AGAGTGAAAA TGAATTCAAG 
ACAAATTGTA CTCGGCTAAC AACGGAGAAG CAAATTCTTC AACAACAGCT TGATGAACTG 
AAAATATTAG CAGAGGAAGA TAAAAGCAAG AATGATGAAA TAGTTAACAA GGTGAGACTT 
GCTGAAGAAA AGTATCAAGA CTTGGAGACC CTGCACAATA AAGTGAGAGC AGACTTTGCT 
AAGGAACTGC AGGATAAACT TGTTGAAAAG GAAAATGAGT TTAAACACCT TTTGGTTAAA 
CAAGAAAACC AGTTGATCTT GTGCTGCAAA AATGCTGAAG ATTTATTTGA AACACTTGCA 
AATGGACTCA CTGAAAACCA CAAATATAAG TTGAATGGAT TGATTGTTAA AATAGCAAAT 
CTTGAAAGCT GCATGCATAA ACTAAAAGAG GTCACTTTAA AACAGCGTAG TAGAATATAT 
GAACAAGAAA AACAACTTGA GCAGTTTTCA AACGAACTTC TTTCAGTAAA CCAATCTCTT 
AATCAAGCAA CTCAGGAGAA AGAATTTGCT ACTTTGTCGC TAAAGAATGA GATTGAAGCT 
CTTAATTTTC AAAATGAGAT GTTAAAAAAA GAAGGAGGAA GTCAACAGCA GGTTGCAGAT 
GAGAAAGAAT CTTGCATTAC TCAGCTGAAA AAAGAACTTT CAGAGAATAT AAATATTGTG 
ACTTCCTTGA CAGAAGTGGT AAAACAGAAA GAATCTGAGG TATCTTCTCT TTCTGCCTTA 
ATTAATGAAC TTAAACTGAA ACATGAGAAT TGTGTAGATT TAACAGAAAA AGAAAATGCA 
ATTGCTTTTC TCACTGCACA GCAGAAACAG AGTCAGCAAG AGCTACAGAA TCAAATTCAG 
GATCTAATGG CAAAGATTGA GGTTCTGAAT AAAGAAAAAT CTGCACAAAT AGATGAGGCT 
GCTAAACTTA AAAATAAGTT GGATGAATGG AAGAAAAAGG CAGAACTTAA ATTTGTGCAG 
AACCACAACA CTATTAAAGA GCTAAAAGAG AAAGTTGAAT CAGTCAATAA GCAAATTGTT 
GAAAAAGATG AAAAACTTAG GACAGTGACA GAAGAATTTG AATGTCATCG TAGCAATATC 
CAAGAAACAC AACTCATGAG TGAAAGCAAA GAATTTGAAT TAACTACCCA GTTGGAAGGC 
CAGAAATCTA AAATTTCGGA ATTGGAGGAC CAGTGTTCGA AGCTGATGTC TGAAAAGAAA 
AGTTTGATGG ATCAAATCGA AATTCAAACT ATTCAGCAGA GTGAAGATAA GAAAGCACTG 
GTTGAGCAAC TCCAGCATAT TCAGTATGCT TCTGACAATA ACTCCAAGGA AGTGCAAAAT 
AAAGTTATAA CACTTGAAAA AGAAATTGAT GCTTTAAAGG AGAAACTTGA TGCAGAGCAA 
ACAAAATGGC AGCTGGAGAA AGCAGAGTGC TTTCAGCTGA AAGATAAGGA ATTAAAAATA 
TTGGAAGAGA AACTAACTGC AGAAAGCACA AATAAACTTT CAGAGCTCAA GAAAAAAGCT 
GAACAGAAAA TCGCTTCTCT TAAGAAACAA CTTATGTCTC AGATAGATGA GAAGGAGCAG 
TCCAGGAAAG CGCTGGAAAG CCAGCTAAAT GTCTTAAAAG ATAATATGCA GAAAGAGAGC 
CAGGAAGTGC AGGAAGGATT GCAACAAAAA GTTGAAAATC AAGACAAAAA ATATGCATTG 
CTACTGGAGC AGAAGAAAAA TACAGAAGCA GATTTAGCTA ATATTAGAAG TGAACTAAAA 
TTAAAGGAAC TAAGAGTTAA AGAAATGGAA GATATTCTTA AAGATGTCCA GCAAAAACAT 
TCTGAAAGAG AGCTGACCGA CAGTGAGAAA GAACAAGCAC TTTTAAAAGA ACTAGAGTCA 
CAGATGCTGA CAAATAATCA GCTGGTTTCT ACTCATGAAA AAGCTTTAAG AGCCCTGCAA 
GATGACCTCA ATGTCAAGGA TGCAGAGATA AAGACCTTTA AAGAGGAAAT AGATGAGAAA 
TCCAAATCCT TAAATGAACT CCAAGTGCTT TTTGAGGAGA CTCAGTCTCA AGAGAGCGCA 
ATGAGAATAA AACTTGAGGA AATGGAGAGT GAGAAGCTAA AATTCAAGAA TGAAATAACT 
AAGCTACAGA AAGATATGCG CTCTTTACGT AAGGAACACA GCCAAGAACT AGATTTGCTT 
AAAAAGGAGA TAACCGAGGA AGCCGAACAA AGAATCAAAT ACGAACAGGA AGACTCGGAA 
CTTAAGCATA ATTCTAAATT AAAGCAACTC ATGAGAGAAT TCAATACCCA GATGGCTCAA 
AAGGAGCGTG AGGTTGAAAC AGCTGTTCAG GAGACTATTG CTAAAGCTCA GGAGGTTGAG 
GCTGAATTAA TGCGATCACA ACAGATAGAA GCAGTCCATC TTCATAAAAA GATTGCTGAG 
AAAGAAGATG ACCTAAAGAG AACTGTAAAA AGATATGAAG AACTTTTAGA GGCCCGAGAA 
GAGGAAATGA CAGCAAAAGT GACAGAGTTA CAAGAGCAAC TTGAAAGATT ACAGGAACAA 
TTAACTCAGA AGGAAATGCA AAATGAAGAA GAAGAAAATG TGGAGGAGGC AACATCTCCT 
GAGGTTTTTC AGGCGCAGCT GGCACAGAAA ACATCGCAGG TCAATGAATA CAAACTGAAG 
GAGCAGGAAC TTAAGGAGAA GGTTCACATC CTAGAAGACC AGGTGAAAAA CTTTACTCGT 
GGTGTATTTT ATACTCCTAT TGGCACACCG TATAAAGAAG TTAATAATCA ACATGCAGGG 
GTTTCTGTTT TTGGCGAACC CACTGAATTT GAGTACTTAA GAAAGGTGAT GTTTGAGTAC 
ATGATGGGGC GTGAAACAAA GACAATGGCA AAGGTAATAA CAACTGTGCT GAGGTTCCCA 
ACAGATCAAG CTCAACAAAT TTTGCAACGA GAAGAAACCC GACCACTGTC TTGGCTTCTA 
TCTTCCTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

golga4 ( XM_018094816.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017950305.1 Xenopus tropicalis golgin A4 (golga4), transcript variant X1, mRNA.

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CloneID	OXa45016
Clone ID Related Accession (Same CDS sequence)	XM_018094816.1 , XM_018094816.2
Accession Version	XM_018094816.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	golgin subfamily A member 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018094816.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAAGA AACTGAAACA GAAGATCAGC GAAGAGCAGT CCCCTTCCAA AAGCCCCCTT 
GCTCCACAGC AGCAGGGTCG TCCAAGAAGC ACATCAACAG GCAACAGAAG TCGGACTTCG 
TCAGTAACAG AACAGCAGGA TGACAGCCTG GCAACAGCAG ATAGAGAGAA CGGATCATTG 
TCGGACAATA TTAATGGAAA TGATCCCACT TCTTCTCAGA GGAATGAACC TCAGTCTTTT 
GCACATAAGC TACAAATGCG GGTTCCTTCA ATGGAATCAC TGTTTCGAAG CCCCATTAAA 
GAATCACTTT TTCCTTCTTC AACTAAAGAA AATCTTTTGC GGAGTAGTTC ACGGGATTCC 
CTGAATAAAA TTGATATGGA GAATCCAAGT ACTGTATTTG ATCCCCCATC CGATATTGAA 
AGTGAAGCTG AGGATTCTGT GGGAAGTATA GAAGGACTTA ACAAAGAACA GCTGTTTAAT 
CGACTACATA GAATGGATAA AAGTTTAGGA AACTACAGAG GAAAATATTC TGAGCTTGTA 
ACAGCCTACA GAACAATGCA GAGAGACAAA GAGAAACTAC AGTCCATCTT GAGTCAGAGC 
CAGGACAAAG CACTAAGGAG AATTGGAGAG CTGCGAGAGG AGCTTCAAAT GGATCAACAG 
GCTAAAAAGC ATCTACAGGA AGAGTTTGAT GCATCTCTGG AAGAGAAAGA TCAACTTATC 
AGTGTTCTGC AAACACAGGT TAGTTTACTT AAAAACAGAC TTCAGAATGG ACAAAGCAGT 
ACTGAATTAC CAGAAAGCAC AGCCTCGCCA GAACTTGACT CTGAGAGTTC CTTGCAAGAG 
GTGGATACAG ATAACCGTTC CACCCCTGAT GATGGGAATG CTGCAAACAC TTTAAATGCT 
CTCCAGGTTA GAGTAAAACG TCAAGAGAAC CTTTTGCAAA GGTGTAAGAA ATCAATCAAG 
TCGCACAAGG AACAGTGTGC TCACCTCAGT AGTGAAAATG AAGCTCTTCA AAACCAACTA 
GATGAAAGAC TCCAGGAACT TGAGAAAATT AAAGAACTTC ACACTAGTGA GAAGAGCAAG 
CTGATTACCC AGCTTAGAGA TGCCAAGAAT TCAATTGAAC AGCTGGAACA AGATAAGGGT 
ATGATGATTG CAGAAACAAA ACGGCAGATG CATGAGACAT TAGAACTGAA GGAAGAAGAG 
GTTTCTCAGC TTCGGTCAAG GGTCAAGAAA TTAAGTTCAC ATTGTGAGGA ACTGCAGCAA 
CAGAAAGAAA AGTTAGAGAA AGCTGCATTT GAAGATGTAG AAAAAATATC TTCACAGAAA 
GCTGAGGATG CTTTTGAAAA GCTGCAGATG GAAGAGCAAA TTAAAGCTAT AGAAAAGGCC 
AGTGAGGAGG AGCGTGTCAA TCTTCAGAAG GAATTAAGTC GTGTGAAGCA AGAGGTGGTG 
GACATTATGA AGAAATCCTG TGATAGTCGT ATTAGTGAGA TTGAGAAAGT TCATGCAGAA 
GCCTTGCTGA ATAAGGAACA GGAGTATAAT GAACAGATTC GCCTGAAGGA ACAAGAATTC 
CAGGATCAAA TGAATGCTGT CAAAAAGAAT GAGGATGAGA CATTAAATTT GCTACAAGAT 
AAAGAAAAGA GTTTAATGGC ACTGGAAGAG CTCGAGCGAC AGAAAAAGAC TATTGAGTCC 
AGAGCAGCAG AACAACTTAA AGAAATGCAA CAAGAAGTTG AAAATTGCAG AATGAGGATT 
CTTGACCTTG AGAGTTCTGT TGCTGAAGAA AGTAAGTCCC AAGATTTTGT AAGCCTGATT 
GAAACAGAGA GAAATAATCA CAATGCAGAA GTAGCTACCA TCAAAGAAAT GCATAAGCAA 
GATCTTGATG TCCTTGTACA GGATCAAGAA AGGATATGGT CAGAAAAGCT TGAGCTGCTT 
AAGAAAGAGA ATCATACTAA GATTATTGAA CTTAAAGAGA AGTATCAGCG AGATTATGAT 
TCTCACTTGA AAGAGAAAGA GCATAATTTC CAGGAGCATA TTGAGCATAT GAATGAAAAA 
ACATTAGAAA AACTAGATGT AAAACAAACT GAATTGGAAA CCCTCTCAGC TGAACTGTCT 
GAGGCTATAA AGGCACGGCA AGAGATTGAG CAGAAACTTA CAGACACAGA AAATGAGATT 
AGCAAAATAA AACTAGAGTA TGAAGCCCAG ATAAAAGAAG AAAGAGAAAA ACACAGTGAA 
GAAATTGGTT CCATTGAAAG AGAGAAGGAA ATGCATACTC AGGGTGTTGA GAAAGACTTG 
AAGGAAAATA TTAACAACCT AAAACTACTT CAAGATAGTA AAGAATGTGA GTTGGAAGAA 
TTCAGAAAGG AAAATCAAAA GCTAAAAGAA GCTGCTGAAA AGGCAGAAGC AGACCATAAA 
GAAGCATTTG CTCTATTGGA AGCAGCCAGG CATTCTCATG AGATAAATAC ACATGAGAAA 
GAACAGGCTT ATGAGCTAAA ATTGAAGGAG CTTCAGGAGA TGCTAAAAGG TATGACGCAG 
GAGCGAAACG ATCTTAAAGA GGTGACAGAA AAAGCAGAAA CACAGTTAAA GAATATGCAG 
ATCGAGCTGG ATTCTAACAA GGCCCAAGTA CAAAGCCTTA CAAAGCAAAT TCATGAGGAA 
AGTATGAATA TGGCAGATAA ACTTAACACT TTGGTACTAG AGTCTGAAAC TCAGGTAAAA 
GGGTTGAAGG AACAAATAGA AGAGATAAGC AGAATAGCTT CAGAGCGTGA ACATGAACTT 
AAAAAAGTAA AAGAAGATCA TATTCAAATT ATTTTAGACC TAAACAATAC TATATCTGAA 
AAAGAGAAGA ATATACTTTC TTTGCAAGAA GATTACAAAG TAAAACACAA AAATCTGGAT 
GCTAAAATGG AAAAATTAAA GCAGAAGTTT AAAGAGCTGC AAGAATCATC TAAAAAGAAA 
TATTCAGAGT TGGAAAGCAA ACTTAAGAAA GAGATCGAGA AGAAGCAGAA CGAGTTGATT 
AACAAAGAAA GGGAGTTTAA TGAAAAAATA CATGAGATGG CCCAAGCAAG TTCAACTGGA 
ATTAATCATG CAATGGCTCA GCTTGAACTG AATCATAAAG AGCAGATTCA GAATATGACA 
GACAATCATC AGAGAGAATT ACAGGAAACA ACAGAAGCTT GTGAAAAGAA ATTAAGCCAA 
CTTGCTGAAG ACCTTAGAGA GAAGCACGAA GAAGAACTTC AGGAGAAAGA TCAGGTCATA 
GCAGAATCAA GAAAACAGTT TAATATCAAG AATAAAGAAA AAGAAGATGC AGACAAAGAG 
ATATCAGACC TCAGGGAACT GAAGACCCAA ATGGATGCCA CGTTTAATGA TTTGCAAGAA 
CAGCTGTGTC AGTCAGCAGC TCAAGTTTTA CATTTGACAC AGAGTGAAAA TGAATTCAAG 
ACAAATTGTA CTCGGCTAAC AACGGAGAAG CAAATTCTTC AACAACAGCT TGATGAACTG 
AAAATATTAG CAGAGGAAGA TAAAAGCAAG AATGATGAAA TAGTTAACAA GGTGAGACTT 
GCTGAAGAAA AGTATCAAGA CTTGGAGACC CTGCACAATA AAGTGAGAGC AGACTTTGCT 
AAGGAACTGC AGGATAAACT TGTTGAAAAG GAAAATGAGT TTAAACACCT TTTGGTTAAA 
CAAGAAAACC AGTTGATCTT GTGCTGCAAA AATGCTGAAG ATTTATTTGA AACACTTGCA 
AATGGACTCA CTGAAAACCA CAAATATAAG TTGAATGGAT TGATTGTTAA AATAGCAAAT 
CTTGAAAGCT GCATGCATAA ACTAAAAGAG GTCACTTTAA AACAGCGTAG TAGAATATAT 
GAACAAGAAA AACAACTTGA GCAGTTTTCA AACGAACTTC TTTCAGTAAA CCAATCTCTT 
AATCAAGCAA CTCAGGAGAA AGAATTTGCT ACTTTGTCGC TAAAGAATGA GATTGAAGCT 
CTTAATTTTC AAAATGAGAT GTTAAAAAAA GAAGGAGGAA GTCAACAGCA GGTTGCAGAT 
GAGAAAGAAT CTTGCATTAC TCAGCTGAAA AAAGAACTTT CAGAGAATAT AAATATTGTG 
ACTTCCTTGA CAGAAGTGGT AAAACAGAAA GAATCTGAGG TATCTTCTCT TTCTGCCTTA 
ATTAATGAAC TTAAACTGAA ACATGAGAAT TGTGTAGATT TAACAGAAAA AGAAAATGCA 
ATTGCTTTTC TCACTGCACA GCAGAAACAG AGTCAGCAAG AGCTACAGAA TCAAATTCAG 
GATCTAATGG CAAAGATTGA GGTTCTGAAT AAAGAAAAAT CTGCACAAAT AGATGAGGCT 
GCTAAACTTA AAAATAAGTT GGATGAATGG AAGAAAAAGG CAGAACTTAA ATTTGTGCAG 
AACCACAACA CTATTAAAGA GCTAAAAGAG AAAGTTGAAT CAGTCAATAA GCAAATTGTT 
GAAAAAGATG AAAAACTTAG GACAGTGACA GAAGAATTTG AATGTCATCG TAGCAATATC 
CAAGAAACAC AACTCATGAG TGAAAGCAAA GAATTTGAAT TAACTACCCA GTTGGAAGGC 
CAGAAATCTA AAATTTCGGA ATTGGAGGAC CAGTGTTCGA AGCTGATGTC TGAAAAGAAA 
AGTTTGATGG ATCAAATCGA AATTCAAACT ATTCAGCAGA GTGAAGATAA GAAAGCACTG 
GTTGAGCAAC TCCAGCATAT TCAGTATGCT TCTGACAATA ACTCCAAGGA AGTGCAAAAT 
AAAGTTATAA CACTTGAAAA AGAAATTGAT GCTTTAAAGG AGAAACTTGA TGCAGAGCAA 
ACAAAATGGC AGCTGGAGAA AGCAGAGTGC TTTCAGCTGA AAGATAAGGA ATTAAAAATA 
TTGGAAGAGA AACTAACTGC AGAAAGCACA AATAAACTTT CAGAGCTCAA GAAAAAAGCT 
GAACAGAAAA TCGCTTCTCT TAAGAAACAA CTTATGTCTC AGATAGATGA GAAGGAGCAG 
TCCAGGAAAG CGCTGGAAAG CCAGCTAAAT GTCTTAAAAG ATAATATGCA GAAAGAGAGC 
CAGGAAGTGC AGGAAGGATT GCAACAAAAA GTTGAAAATC AAGACAAAAA ATATGCATTG 
CTACTGGAGC AGAAGAAAAA TACAGAAGCA GATTTAGCTA ATATTAGAAG TGAACTAAAA 
TTAAAGGAAC TAAGAGTTAA AGAAATGGAA GATATTCTTA AAGATGTCCA GCAAAAACAT 
TCTGAAAGAG AGCTGACCGA CAGTGAGAAA GAACAAGCAC TTTTAAAAGA ACTAGAGTCA 
CAGATGCTGA CAAATAATCA GCTGGTTTCT ACTCATGAAA AAGCTTTAAG AGCCCTGCAA 
GATGACCTCA ATGTCAAGGA TGCAGAGATA AAGACCTTTA AAGAGGAAAT AGATGAGAAA 
TCCAAATCCT TAAATGAACT CCAAGTGCTT TTTGAGGAGA CTCAGTCTCA AGAGAGCGCA 
ATGAGAATAA AACTTGAGGA AATGGAGAGT GAGAAGCTAA AATTCAAGAA TGAAATAACT 
AAGCTACAGA AAGATATGCG CTCTTTACGT AAGGAACACA GCCAAGAACT AGATTTGCTT 
AAAAAGGAGA TAACCGAGGA AGCCGAACAA AGAATCAAAT ACGAACAGGA AGACTCGGAA 
CTTAAGCATA ATTCTAAATT AAAGCAACTC ATGAGAGAAT TCAATACCCA GATGGCTCAA 
AAGGAGCGTG AGGTTGAAAC AGCTGTTCAG GAGACTATTG CTAAAGCTCA GGAGGTTGAG 
GCTGAATTAA TGCGATCACA ACAGATAGAA GCAGTCCATC TTCATAAAAA GATTGCTGAG 
AAAGAAGATG ACCTAAAGAG AACTGTAAAA AGATATGAAG AACTTTTAGA GGCCCGAGAA 
GAGGAAATGA CAGCAAAAGT GACAGAGTTA CAAGAGCAAC TTGAAAGATT ACAGGAACAA 
TTAACTCAGA AGGAAATGCA AAATGAAGAA GAAGAAAATG TGGAGGAGGC AACATCTCCT 
GAGGTTTTTC AGGCGCAGCT GGCACAGAAA ACATCGCAGG TCAATGAATA CAAACTGAAG 
GAGCAGGAAC TTAAGGAGAA GGTTCACATC CTAGAAGACC AGGTGAAAAA CTTTACTCGT 
GGTGTATTTT ATACTCCTAT TGGCACACCG TATAAAGAAG TTAATAATCA ACATGCAGGG 
GTTTCTGTTT TTGGCGAACC CACTGAATTT GAGTACTTAA GAAAGGTGAT GTTTGAGTAC 
ATGATGGGGC GTGAAACAAA GACAATGGCA AAGGTAATAA CAACTGTGCT GAGGTTCCCA 
ACAGATCAAG CTCAACAAAT TTTGCAACGA GAAGAAACCC GACCACTGTC TTGGCTTCTA 
TCTTCCTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017950305.1
CDS	219..6590
Translation	MFKKLKQKISEEQSPSKSPLAPQQQGRPRSTSTGNRSRTSSVTEQQDDSLATADRENGSLSDNINGNDPTSSQRNEPQSFAHKLQMRVPSMESLFRSPIKESLFPSSTKENLLRSSSRDSLNKIDMENPSTVFDPPSDIESEAEDSVGSIEGLNKEQLFNRLHRMDKSLGNYRGKYSELVTAYRTMQRDKEKLQSILSQSQDKALRRIGELREELQMDQQAKKHLQEEFDASLEEKDQLISVLQTQVSLLKNRLQNGQSSTELPESTASPELDSESSLQEVDTDNRSTPDDGNAANTLNALQVRVKRQENLLQRCKKSIKSHKEQCAHLSSENEALQNQLDERLQELEKIKELHTSEKSKLITQLRDAKNSIEQLEQDKGMMIAETKRQMHETLELKEEEVSQLRSRVKKLSSHCEELQQQKEKLEKAAFEDVEKISSQKAEDAFEKLQMEEQIKAIEKASEEERVNLQKELSRVKQEVVDIMKKSCDSRISEIEKVHAEALLNKEQEYNEQIRLKEQEFQDQMNAVKKNEDETLNLLQDKEKSLMALEELERQKKTIESRAAEQLKEMQQEVENCRMRILDLESSVAEESKSQDFVSLIETERNNHNAEVATIKEMHKQDLDVLVQDQERIWSEKLELLKKENHTKIIELKEKYQRDYDSHLKEKEHNFQEHIEHMNEKTLEKLDVKQTELETLSAELSEAIKARQEIEQKLTDTENEISKIKLEYEAQIKEEREKHSEEIGSIEREKEMHTQGVEKDLKENINNLKLLQDSKECELEEFRKENQKLKEAAEKAEADHKEAFALLEAARHSHEINTHEKEQAYELKLKELQEMLKGMTQERNDLKEVTEKAETQLKNMQIELDSNKAQVQSLTKQIHEESMNMADKLNTLVLESETQVKGLKEQIEEISRIASEREHELKKVKEDHIQIILDLNNTISEKEKNILSLQEDYKVKHKNLDAKMEKLKQKFKELQESSKKKYSELESKLKKEIEKKQNELINKEREFNEKIHEMAQASSTGINHAMAQLELNHKEQIQNMTDNHQRELQETTEACEKKLSQLAEDLREKHEEELQEKDQVIAESRKQFNIKNKEKEDADKEISDLRELKTQMDATFNDLQEQLCQSAAQVLHLTQSENEFKTNCTRLTTEKQILQQQLDELKILAEEDKSKNDEIVNKVRLAEEKYQDLETLHNKVRADFAKELQDKLVEKENEFKHLLVKQENQLILCCKNAEDLFETLANGLTENHKYKLNGLIVKIANLESCMHKLKEVTLKQRSRIYEQEKQLEQFSNELLSVNQSLNQATQEKEFATLSLKNEIEALNFQNEMLKKEGGSQQQVADEKESCITQLKKELSENINIVTSLTEVVKQKESEVSSLSALINELKLKHENCVDLTEKENAIAFLTAQQKQSQQELQNQIQDLMAKIEVLNKEKSAQIDEAAKLKNKLDEWKKKAELKFVQNHNTIKELKEKVESVNKQIVEKDEKLRTVTEEFECHRSNIQETQLMSESKEFELTTQLEGQKSKISELEDQCSKLMSEKKSLMDQIEIQTIQQSEDKKALVEQLQHIQYASDNNSKEVQNKVITLEKEIDALKEKLDAEQTKWQLEKAECFQLKDKELKILEEKLTAESTNKLSELKKKAEQKIASLKKQLMSQIDEKEQSRKALESQLNVLKDNMQKESQEVQEGLQQKVENQDKKYALLLEQKKNTEADLANIRSELKLKELRVKEMEDILKDVQQKHSERELTDSEKEQALLKELESQMLTNNQLVSTHEKALRALQDDLNVKDAEIKTFKEEIDEKSKSLNELQVLFEETQSQESAMRIKLEEMESEKLKFKNEITKLQKDMRSLRKEHSQELDLLKKEITEEAEQRIKYEQEDSELKHNSKLKQLMREFNTQMAQKEREVETAVQETIAKAQEVEAELMRSQQIEAVHLHKKIAEKEDDLKRTVKRYEELLEAREEEMTAKVTELQEQLERLQEQLTQKEMQNEEEENVEEATSPEVFQAQLAQKTSQVNEYKLKEQELKEKVHILEDQVKNFTRGVFYTPIGTPYKEVNNQHAGVSVFGEPTEFEYLRKVMFEYMMGRETKTMAKVITTVLRFPTDQAQQILQREETRPLSWLLSSS

Target ORF information:

RefSeq Version	XM_018094816.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis golgin A4 (golga4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094816.2

ORF Insert Sequence:

ATGTTTAAGA AACTGAAACA GAAGATCAGC GAAGAGCAGT CCCCTTCCAA AAGCCCCCTT 
GCTCCACAGC AGCAGGGTCG TCCAAGAAGC ACATCAACAG GCAACAGAAG TCGGACTTCG 
TCAGTAACAG AACAGCAGGA TGACAGCCTG GCAACAGCAG ATAGAGAGAA CGGATCATTG 
TCGGACAATA TTAATGGAAA TGATCCCACT TCTTCTCAGA GGAATGAACC TCAGTCTTTT 
GCACATAAGC TACAAATGCG GGTTCCTTCA ATGGAATCAC TGTTTCGAAG CCCCATTAAA 
GAATCACTTT TTCCTTCTTC AACTAAAGAA AATCTTTTGC GGAGTAGTTC ACGGGATTCC 
CTGAATAAAA TTGATATGGA GAATCCAAGT ACTGTATTTG ATCCCCCATC CGATATTGAA 
AGTGAAGCTG AGGATTCTGT GGGAAGTATA GAAGGACTTA ACAAAGAACA GCTGTTTAAT 
CGACTACATA GAATGGATAA AAGTTTAGGA AACTACAGAG GAAAATATTC TGAGCTTGTA 
ACAGCCTACA GAACAATGCA GAGAGACAAA GAGAAACTAC AGTCCATCTT GAGTCAGAGC 
CAGGACAAAG CACTAAGGAG AATTGGAGAG CTGCGAGAGG AGCTTCAAAT GGATCAACAG 
GCTAAAAAGC ATCTACAGGA AGAGTTTGAT GCATCTCTGG AAGAGAAAGA TCAACTTATC 
AGTGTTCTGC AAACACAGGT TAGTTTACTT AAAAACAGAC TTCAGAATGG ACAAAGCAGT 
ACTGAATTAC CAGAAAGCAC AGCCTCGCCA GAACTTGACT CTGAGAGTTC CTTGCAAGAG 
GTGGATACAG ATAACCGTTC CACCCCTGAT GATGGGAATG CTGCAAACAC TTTAAATGCT 
CTCCAGGTTA GAGTAAAACG TCAAGAGAAC CTTTTGCAAA GGTGTAAGAA ATCAATCAAG 
TCGCACAAGG AACAGTGTGC TCACCTCAGT AGTGAAAATG AAGCTCTTCA AAACCAACTA 
GATGAAAGAC TCCAGGAACT TGAGAAAATT AAAGAACTTC ACACTAGTGA GAAGAGCAAG 
CTGATTACCC AGCTTAGAGA TGCCAAGAAT TCAATTGAAC AGCTGGAACA AGATAAGGGT 
ATGATGATTG CAGAAACAAA ACGGCAGATG CATGAGACAT TAGAACTGAA GGAAGAAGAG 
GTTTCTCAGC TTCGGTCAAG GGTCAAGAAA TTAAGTTCAC ATTGTGAGGA ACTGCAGCAA 
CAGAAAGAAA AGTTAGAGAA AGCTGCATTT GAAGATGTAG AAAAAATATC TTCACAGAAA 
GCTGAGGATG CTTTTGAAAA GCTGCAGATG GAAGAGCAAA TTAAAGCTAT AGAAAAGGCC 
AGTGAGGAGG AGCGTGTCAA TCTTCAGAAG GAATTAAGTC GTGTGAAGCA AGAGGTGGTG 
GACATTATGA AGAAATCCTG TGATAGTCGT ATTAGTGAGA TTGAGAAAGT TCATGCAGAA 
GCCTTGCTGA ATAAGGAACA GGAGTATAAT GAACAGATTC GCCTGAAGGA ACAAGAATTC 
CAGGATCAAA TGAATGCTGT CAAAAAGAAT GAGGATGAGA CATTAAATTT GCTACAAGAT 
AAAGAAAAGA GTTTAATGGC ACTGGAAGAG CTCGAGCGAC AGAAAAAGAC TATTGAGTCC 
AGAGCAGCAG AACAACTTAA AGAAATGCAA CAAGAAGTTG AAAATTGCAG AATGAGGATT 
CTTGACCTTG AGAGTTCTGT TGCTGAAGAA AGTAAGTCCC AAGATTTTGT AAGCCTGATT 
GAAACAGAGA GAAATAATCA CAATGCAGAA GTAGCTACCA TCAAAGAAAT GCATAAGCAA 
GATCTTGATG TCCTTGTACA GGATCAAGAA AGGATATGGT CAGAAAAGCT TGAGCTGCTT 
AAGAAAGAGA ATCATACTAA GATTATTGAA CTTAAAGAGA AGTATCAGCG AGATTATGAT 
TCTCACTTGA AAGAGAAAGA GCATAATTTC CAGGAGCATA TTGAGCATAT GAATGAAAAA 
ACATTAGAAA AACTAGATGT AAAACAAACT GAATTGGAAA CCCTCTCAGC TGAACTGTCT 
GAGGCTATAA AGGCACGGCA AGAGATTGAG CAGAAACTTA CAGACACAGA AAATGAGATT 
AGCAAAATAA AACTAGAGTA TGAAGCCCAG ATAAAAGAAG AAAGAGAAAA ACACAGTGAA 
GAAATTGGTT CCATTGAAAG AGAGAAGGAA ATGCATACTC AGGGTGTTGA GAAAGACTTG 
AAGGAAAATA TTAACAACCT AAAACTACTT CAAGATAGTA AAGAATGTGA GTTGGAAGAA 
TTCAGAAAGG AAAATCAAAA GCTAAAAGAA GCTGCTGAAA AGGCAGAAGC AGACCATAAA 
GAAGCATTTG CTCTATTGGA AGCAGCCAGG CATTCTCATG AGATAAATAC ACATGAGAAA 
GAACAGGCTT ATGAGCTAAA ATTGAAGGAG CTTCAGGAGA TGCTAAAAGG TATGACGCAG 
GAGCGAAACG ATCTTAAAGA GGTGACAGAA AAAGCAGAAA CACAGTTAAA GAATATGCAG 
ATCGAGCTGG ATTCTAACAA GGCCCAAGTA CAAAGCCTTA CAAAGCAAAT TCATGAGGAA 
AGTATGAATA TGGCAGATAA ACTTAACACT TTGGTACTAG AGTCTGAAAC TCAGGTAAAA 
GGGTTGAAGG AACAAATAGA AGAGATAAGC AGAATAGCTT CAGAGCGTGA ACATGAACTT 
AAAAAAGTAA AAGAAGATCA TATTCAAATT ATTTTAGACC TAAACAATAC TATATCTGAA 
AAAGAGAAGA ATATACTTTC TTTGCAAGAA GATTACAAAG TAAAACACAA AAATCTGGAT 
GCTAAAATGG AAAAATTAAA GCAGAAGTTT AAAGAGCTGC AAGAATCATC TAAAAAGAAA 
TATTCAGAGT TGGAAAGCAA ACTTAAGAAA GAGATCGAGA AGAAGCAGAA CGAGTTGATT 
AACAAAGAAA GGGAGTTTAA TGAAAAAATA CATGAGATGG CCCAAGCAAG TTCAACTGGA 
ATTAATCATG CAATGGCTCA GCTTGAACTG AATCATAAAG AGCAGATTCA GAATATGACA 
GACAATCATC AGAGAGAATT ACAGGAAACA ACAGAAGCTT GTGAAAAGAA ATTAAGCCAA 
CTTGCTGAAG ACCTTAGAGA GAAGCACGAA GAAGAACTTC AGGAGAAAGA TCAGGTCATA 
GCAGAATCAA GAAAACAGTT TAATATCAAG AATAAAGAAA AAGAAGATGC AGACAAAGAG 
ATATCAGACC TCAGGGAACT GAAGACCCAA ATGGATGCCA CGTTTAATGA TTTGCAAGAA 
CAGCTGTGTC AGTCAGCAGC TCAAGTTTTA CATTTGACAC AGAGTGAAAA TGAATTCAAG 
ACAAATTGTA CTCGGCTAAC AACGGAGAAG CAAATTCTTC AACAACAGCT TGATGAACTG 
AAAATATTAG CAGAGGAAGA TAAAAGCAAG AATGATGAAA TAGTTAACAA GGTGAGACTT 
GCTGAAGAAA AGTATCAAGA CTTGGAGACC CTGCACAATA AAGTGAGAGC AGACTTTGCT 
AAGGAACTGC AGGATAAACT TGTTGAAAAG GAAAATGAGT TTAAACACCT TTTGGTTAAA 
CAAGAAAACC AGTTGATCTT GTGCTGCAAA AATGCTGAAG ATTTATTTGA AACACTTGCA 
AATGGACTCA CTGAAAACCA CAAATATAAG TTGAATGGAT TGATTGTTAA AATAGCAAAT 
CTTGAAAGCT GCATGCATAA ACTAAAAGAG GTCACTTTAA AACAGCGTAG TAGAATATAT 
GAACAAGAAA AACAACTTGA GCAGTTTTCA AACGAACTTC TTTCAGTAAA CCAATCTCTT 
AATCAAGCAA CTCAGGAGAA AGAATTTGCT ACTTTGTCGC TAAAGAATGA GATTGAAGCT 
CTTAATTTTC AAAATGAGAT GTTAAAAAAA GAAGGAGGAA GTCAACAGCA GGTTGCAGAT 
GAGAAAGAAT CTTGCATTAC TCAGCTGAAA AAAGAACTTT CAGAGAATAT AAATATTGTG 
ACTTCCTTGA CAGAAGTGGT AAAACAGAAA GAATCTGAGG TATCTTCTCT TTCTGCCTTA 
ATTAATGAAC TTAAACTGAA ACATGAGAAT TGTGTAGATT TAACAGAAAA AGAAAATGCA 
ATTGCTTTTC TCACTGCACA GCAGAAACAG AGTCAGCAAG AGCTACAGAA TCAAATTCAG 
GATCTAATGG CAAAGATTGA GGTTCTGAAT AAAGAAAAAT CTGCACAAAT AGATGAGGCT 
GCTAAACTTA AAAATAAGTT GGATGAATGG AAGAAAAAGG CAGAACTTAA ATTTGTGCAG 
AACCACAACA CTATTAAAGA GCTAAAAGAG AAAGTTGAAT CAGTCAATAA GCAAATTGTT 
GAAAAAGATG AAAAACTTAG GACAGTGACA GAAGAATTTG AATGTCATCG TAGCAATATC 
CAAGAAACAC AACTCATGAG TGAAAGCAAA GAATTTGAAT TAACTACCCA GTTGGAAGGC 
CAGAAATCTA AAATTTCGGA ATTGGAGGAC CAGTGTTCGA AGCTGATGTC TGAAAAGAAA 
AGTTTGATGG ATCAAATCGA AATTCAAACT ATTCAGCAGA GTGAAGATAA GAAAGCACTG 
GTTGAGCAAC TCCAGCATAT TCAGTATGCT TCTGACAATA ACTCCAAGGA AGTGCAAAAT 
AAAGTTATAA CACTTGAAAA AGAAATTGAT GCTTTAAAGG AGAAACTTGA TGCAGAGCAA 
ACAAAATGGC AGCTGGAGAA AGCAGAGTGC TTTCAGCTGA AAGATAAGGA ATTAAAAATA 
TTGGAAGAGA AACTAACTGC AGAAAGCACA AATAAACTTT CAGAGCTCAA GAAAAAAGCT 
GAACAGAAAA TCGCTTCTCT TAAGAAACAA CTTATGTCTC AGATAGATGA GAAGGAGCAG 
TCCAGGAAAG CGCTGGAAAG CCAGCTAAAT GTCTTAAAAG ATAATATGCA GAAAGAGAGC 
CAGGAAGTGC AGGAAGGATT GCAACAAAAA GTTGAAAATC AAGACAAAAA ATATGCATTG 
CTACTGGAGC AGAAGAAAAA TACAGAAGCA GATTTAGCTA ATATTAGAAG TGAACTAAAA 
TTAAAGGAAC TAAGAGTTAA AGAAATGGAA GATATTCTTA AAGATGTCCA GCAAAAACAT 
TCTGAAAGAG AGCTGACCGA CAGTGAGAAA GAACAAGCAC TTTTAAAAGA ACTAGAGTCA 
CAGATGCTGA CAAATAATCA GCTGGTTTCT ACTCATGAAA AAGCTTTAAG AGCCCTGCAA 
GATGACCTCA ATGTCAAGGA TGCAGAGATA AAGACCTTTA AAGAGGAAAT AGATGAGAAA 
TCCAAATCCT TAAATGAACT CCAAGTGCTT TTTGAGGAGA CTCAGTCTCA AGAGAGCGCA 
ATGAGAATAA AACTTGAGGA AATGGAGAGT GAGAAGCTAA AATTCAAGAA TGAAATAACT 
AAGCTACAGA AAGATATGCG CTCTTTACGT AAGGAACACA GCCAAGAACT AGATTTGCTT 
AAAAAGGAGA TAACCGAGGA AGCCGAACAA AGAATCAAAT ACGAACAGGA AGACTCGGAA 
CTTAAGCATA ATTCTAAATT AAAGCAACTC ATGAGAGAAT TCAATACCCA GATGGCTCAA 
AAGGAGCGTG AGGTTGAAAC AGCTGTTCAG GAGACTATTG CTAAAGCTCA GGAGGTTGAG 
GCTGAATTAA TGCGATCACA ACAGATAGAA GCAGTCCATC TTCATAAAAA GATTGCTGAG 
AAAGAAGATG ACCTAAAGAG AACTGTAAAA AGATATGAAG AACTTTTAGA GGCCCGAGAA 
GAGGAAATGA CAGCAAAAGT GACAGAGTTA CAAGAGCAAC TTGAAAGATT ACAGGAACAA 
TTAACTCAGA AGGAAATGCA AAATGAAGAA GAAGAAAATG TGGAGGAGGC AACATCTCCT 
GAGGTTTTTC AGGCGCAGCT GGCACAGAAA ACATCGCAGG TCAATGAATA CAAACTGAAG 
GAGCAGGAAC TTAAGGAGAA GGTTCACATC CTAGAAGACC AGGTGAAAAA CTTTACTCGT 
GGTGTATTTT ATACTCCTAT TGGCACACCG TATAAAGAAG TTAATAATCA ACATGCAGGG 
GTTTCTGTTT TTGGCGAACC CACTGAATTT GAGTACTTAA GAAAGGTGAT GTTTGAGTAC 
ATGATGGGGC GTGAAACAAA GACAATGGCA AAGGTAATAA CAACTGTGCT GAGGTTCCCA 
ACAGATCAAG CTCAACAAAT TTTGCAACGA GAAGAAACCC GACCACTGTC TTGGCTTCTA 
TCTTCCTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

golga4 ( XM_018094817.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017950306.1 Xenopus tropicalis golgin A4 (golga4), transcript variant X2, mRNA.

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CloneID	OXa45017
Clone ID Related Accession (Same CDS sequence)	XM_018094817.1 , XM_018094817.2
Accession Version	XM_018094817.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	golgin subfamily A member 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAAGA AACTGAAACA GAAGATCAGC GAAGAGCAGT CCCCTTCCAA AAGCCCCCTT 
GCTCCACAGC AGCAGGGTCG TCCAAGAAGC ACATCAACAG GCAACAGAAG TCGGACTTCG 
TCAGTAACAG AACAGCAGGA TGACAGCCTG GCAACAGCAG ATAGAGAGAA CGGATCATTG 
TCGGACAATA TTAATGGAAA TGATCCCACT TCTTCTCAGA GGAATGAACC TCAGTCTTTT 
GCACATAAGC TACAAATGCG GGTTCCTTCA ATGGAATCAC TGTTTCGAAG CCCCATTAAA 
GAATCACTTT TTCCTTCTTC AACTAAAGAA AATCTTTTGC GGAGTAGTTC ACGGGATTCC 
CTGAATAAAA TTGATATGGA GAATCCAAGT ACTGTATTTG ATCCCCCATC CGATATTGAA 
AGTGAAGCTG AGGATTCTGT GGGAAGTATA GAAGGACTTA ACAAAGAACA GCTGTTTAAT 
CGACTACATA GAATGGATAA AAGTTTAGGA AACTACAGAG GAAAATATTC TGAGCTTGTA 
ACAGCCTACA GAACAATGCA GAGAGACAAA GAGAAACTAC AGTCCATCTT GAGTCAGAGC 
CAGGACAAAG CACTAAGGAG AATTGGAGAG CTGCGAGAGG AGCTTCAAAT GGATCAACAG 
GCTAAAAAGC ATCTACAGGA AGAGTTTGAT GCATCTCTGG AAGAGAAAGA TCAACTTATC 
AGTGTTCTGC AAACACAGGT TAGTTTACTT AAAAACAGAC TTCAGAATGG ACAAAGCAGT 
ACTGAATTAC CAGAAAGCAC AGCCTCGCCA GAACTTGACT CTGAGAGTTC CTTGCAAGAG 
GTGGATACAG ATAACCGTTC CACCCCTGAT GATGGGAATG CTGCAAACAC TTTAAATGCT 
CTCCAGGTTA GAGTAAAACG TCAAGAGAAC CTTTTGCAAA GGTGTAAGAA ATCAATCAAG 
TCGCACAAGG AACAGTGTGC TCACCTCAGT AGTGAAAATG AAGCTCTTCA AAACCAACTA 
GATGAAAGAC TCCAGGAACT TGAGAAAATT AAAGAACTTC ACACTAGTGA GAAGAGCAAG 
CTGATTACCC AGCTTAGAGA TGCCAAGAAT TCAATTGAAC AGCTGGAACA AGATAAGGGT 
ATGATGATTG CAGAAACAAA ACGGCAGATG CATGAGACAT TAGAACTGAA GGAAGAAGAG 
GTTTCTCAGC TTCGGTCAAG GGTCAAGAAA TTAAGTTCAC ATTGTGAGGA ACTGCAGCAA 
CAGAAAGAAA AGTTAGAGAA AGCTGCATTT GAAGATGTAG AAAAAATATC TTCACAGAAA 
GCTGAGGATG CTTTTGAAAA GCTGCAGATG GAAGAGCAAA TTAAAGCTAT AGAAAAGGCC 
AGTGAGGAGG AGCGTGTCAA TCTTCAGAAG GAATTAAGTC GTGTGAAGCA AGAGGTGGTG 
GACATTATGA AGAAATCCTG TGATAGTCGT ATTAGTGAGA TTGAGAAAGT TCATGCAGAA 
GCCTTGCTGA ATAAGGAACA GGAGTATAAT GAACAGATTC GCCTGAAGGA ACAAGAATTC 
CAGGATCAAA TGAATGCTGT CAAAAAGAAT GAGGATGAGA CATTAAATTT GCTACAAGAT 
AAAGAAAAGA GTTTAATGGC ACTGGAAGAG CTCGAGCGAC AGAAAAAGAC TATTGAGTCC 
AGAGCAGCAG AACAACTTAA AGAAATGCAA CAAGAAGTTG AAAATTGCAG AATGAGGATT 
CTTGACCTTG AGAGTTCTGT TGCTGAAGAA AGTAAGTCCC AAGATTTTGT AAGCCTGATT 
GAAACAGAGA GAAATAATCA CAATGCAGAA GTAGCTACCA TCAAAGAAAT GCATAAGCAA 
GATCTTGATG TCCTTGTACA GGATCAAGAA AGGATATGGT CAGAAAAGCT TGAGCTGCTT 
AAGAAAGAGA ATCATACTAA GATTATTGAA CTTAAAGAGA AGTATCAGCG AGATTATGAT 
TCTCACTTGA AAGAGAAAGA GCATAATTTC CAGGAGCATA TTGAGCATAT GAATGAAAAA 
ACATTAGAAA AACTAGATGT AAAACAAACT GAATTGGAAA CCCTCTCAGC TGAACTGTCT 
GAGGCTATAA AGGCACGGCA AGAGATTGAG CAGAAACTTA CAGACACAGA AAATGAGATT 
AGCAAAATAA AACTAGAGTA TGAAGCCCAG ATAAAAGAAG AAAGAGAAAA ACACAGTGAA 
GAAATTGGTT CCATTGAAAG AGAGAAGGAA ATGCATACTC AGGGTGTTGA GAAAGACTTG 
AAGGAAAATA TTAACAACCT AAAACTACTT CAAGATAGTA AAGAATGTGA GTTGGAAGAA 
TTCAGAAAGG AAAATCAAAA GCTAAAAGAA GCTGCTGAAA AGGCAGAAGC AGACCATAAA 
GAAGCATTTG CTCTATTGGA AGCAGCCAGG CATTCTCATG AGATAAATAC ACATGAGAAA 
GAACAGGCTT ATGAGCTAAA ATTGAAGGAG CTTCAGGAGA TGCTAAAAGG TATGACGCAG 
GAGCGAAACG ATCTTAAAGA GGTGACAGAA AAAGCAGAAA CACAGTTAAA GAATATGCAG 
ATCGAGCTGG ATTCTAACAA GGCCCAAGTA CAAAGCCTTA CAAAGCAAAT TCATGAGGAA 
AGTATGAATA TGGCAGATAA ACTTAACACT TTGGTACTAG AGTCTGAAAC TCAGGTAAAA 
GGGTTGAAGG AACAAATAGA AGAGATAAGC AGAATAGCTT CAGAGCGTGA ACATGAACTT 
AAAAAAGTAA AAGAAGATCA TATTCAAATT ATTTTAGACC TAAACAATAC TATATCTGAA 
AAAGAGAAGA ATATACTTTC TTTGCAAGAA GATTACAAAG TAAAACACAA AAATCTGGAT 
GCTAAAATGG AAAAATTAAA GCAGAAGTTT AAAGAGCTGC AAGAATCATC TAAAAAGAAA 
TATTCAGAGT TGGAAAGCAA ACTTAAGAAA GAGATCGAGA AGAAGCAGAA CGAGTTGATT 
AACAAAGAAA GGGAGTTTAA TGAAAAAATA CATGAGATGG CCCAAGCAAG TTCAACTGGA 
ATTAATCATG CAATGGCTCA GCTTGAACTG AATCATAAAG AGCAGATTCA GAATATGACA 
GACAATCATC AGAGAGAATT ACAGGAAACA ACAGAAGCTT GTGAAAAGAA ATTAAGCCAA 
CTTGCTGAAG ACCTTAGAGA GAAGCACGAA GAAGAACTTC AGGAGAAAGA TCAGGTCATA 
GCAGAATCAA GAAAACAGTT TAATATCAAG AATAAAGAAA AAGAAGATGC AGACAAAGAG 
ATATCAGACC TCAGGGAACT GAAGACCCAA ATGGATGCCA CGTTTAATGA TTTGCAAGAA 
CAGCTGTGTC AGTCAGCAGC TCAAGTTTTA CATTTGACAC AGAGTGAAAA TGAATTCAAG 
ACAAATTGTA CTCGGCTAAC AACGGAGAAG CAAATTCTTC AACAACAGCT TGATGAACTG 
AAAATATTAG CAGAGGAAGA TAAAAGCAAG AATGATGAAA TAGTTAACAA GGTGAGACTT 
GCTGAAGAAA AGTATCAAGA CTTGGAGACC CTGCACAATA AAGTGAGAGC AGACTTTGCT 
AAGGAACTGC AGGATAAACT TGTTGAAAAG GAAAATGAGT TTAAACACCT TTTGGTTAAA 
CAAGAAAACC AGTTGATCTT GTGCTGCAAA AATGCTGAAG ATTTATTTGA AACACTTGCA 
AATGGACTCA CTGAAAACCA CAAATATAAG TTGAATGGAT TGATTGTTAA AATAGCAAAT 
CTTGAAAGCT GCATGCATAA ACTAAAAGAG GTCACTTTAA AACAGCGTAG TAGAATATAT 
GAACAAGAAA AACAACTTGA GCAGTTTTCA AACGAACTTC TTTCAGTAAA CCAATCTCTT 
AATCAAGCAA CTCAGGAGAA AGAATTTGCT ACTTTGTCGC TAAAGAATGA GATTGAAGCT 
CTTAATTTTC AAAATGAGAT GTTAAAAAAA GAAGGAGGAA GTCAACAGCA GGTTGCAGAT 
GAGAAAGAAT CTTGCATTAC TCAGCTGAAA AAAGAACTTT CAGAGAATAT AAATATTGTG 
ACTTCCTTGA CAGAAGTGGT AAAACAGAAA GAATCTGAGG TATCTTCTCT TTCTGCCTTA 
ATTAATGAAC TTAAACTGAA ACATGAGAAT TGTGTAGATT TAACAGAAAA AGAAAATGCA 
ATTGCTTTTC TCACTGCACA GCAGAAACAG AGTCAGCAAG AGCTACAGAA TCAAATTCAG 
GATCTAATGG CAAAGATTGA GGTTCTGAAT AAAGAAAAAT CTGCACAAAT AGATGAGGCT 
GCTAAACTTA AAAATAAGTT GGATGAATGG AAGAAAAAGG CAGAACTTAA ATTTGTGCAG 
AACCACAACA CTATTAAAGA GCTAAAAGAG AAAGTTGAAT CAGTCAATAA GCAAATTGTT 
GAAAAAGATG AAAAACTTAG GACAGTGACA GAAGAATTTG AATGTCATCG TAGCAATATC 
CAAGAAACAC AACTCATGAG TGAAAGCAAA GAATTTGAAT TAACTACCCA GTTGGAAGGC 
CAGAAATCTA AAATTTCGGA ATTGGAGGAC CAGTGTTCGA AGCTGATGTC TGAAAAGAAA 
AGTTTGATGG ATCAAATCGA AATTCAAACT ATTCAGCAGA GTGAAGATAA GAAAGCACTG 
GTTGAGCAAC TCCAGCATAT TCAGTATGCT TCTGACAATA ACTCCAAGGA AGTGCAAAAT 
AAAGTTATAA CACTTGAAAA AGAAATTGAT GCTTTAAAGG AGAAACTTGA TGCAGAGCAA 
ACAAAATGGC AGCTGGAGAA AGCAGAGTGC TTTCAGCTGA AAGATAAGGA ATTAAAAATA 
TTGGAAGAGA AACTAACTGC AGAAAGCACA AATAAACTTT CAGAGCTCAA GAAAAAAGCT 
GAACAGAAAA TCGCTTCTCT TAAGAAACAA CTTATGTCTC AGATAGATGA GAAGGAGCAG 
TCCAGGAAAG CGCTGGAAAG CCAGCTAAAT GTCTTAAAAG ATAATATGCA GAAAGAGAGC 
CAGGAAGTGC AGGAAGGATT GCAACAAAAA GTTGAAAATC AAGACAAAAA ATATGCATTG 
CTACTGGAGC AGAAGAAAAA TACAGAAGCA GATTTAGCTA ATATTAGAAG TGAACTAAAA 
TTAAAGGAAC TAAGAGTTAA AGAAATGGAA GATATTCTTA AAGATGTCCA GCAAAAACAT 
TCTGAAAGAG AGCTGACCGA CAGTGAGAAA GAACAAGCAC TTTTAAAAGA ACTAGAGTCA 
CAGATGCTGA CAAATAATCA GCTGGTTTCT ACTCATGAAA AAGCTTTAAG AGCCCTGCAA 
GATGACCTCA ATGTCAAGGA TGCAGAGATA AAGACCTTTA AAGAGGAAAT AGATGAGAAA 
TCCAAATCCT TAAATGAACT CCAAGTGCTT TTTGAGGAGA CTCAGTCTCA AGAGAGCGCA 
ATGAGAATAA AACTTGAGGA AATGGAGAGT GAGAAGCTAA AATTCAAGAA TGAAATAACT 
AAGCTACAGA AAGATATGCG CTCTTTACGT AAGGAACACA GCCAAGAACT AGATTTGCTT 
AAAAAGGAGA TAACCGAGGA AGCCGAACAA AGAATCAAAT ACGAACAGGA AGACTCGGAA 
CTTAAGCATA ATTCTAAATT AAAGCAACTC ATGAGAGAAT TCAATACCCA GATGGCTCAA 
AAGGAGCGTG AGGTTGAAAC AGCTGTTCAG GAGACTATTG CTAAAGCTCA GGAGGTTGAG 
GCTGAATTAA TGCGATCACA ACAGATAGAA GCAGTCCATC TTCATAAAAA GATTGCTGAG 
AAAGAAGATG ACCTAAAGAG AACTGTAAAA AGATATGAAG AACTTTTAGA GGCCCGAGAA 
GAGGAAATGA CAGCAAAAGT GACAGAGTTA CAAGAGCAAC TTGAAAGATT ACAGGAACAA 
TTAACTCAGA AGGAAATGCA AAATGAAGAA GAAGAAAATG TGGAGGAGGC AACATCTCCT 
GAGGTTTTTC AGGCGCAGCT GGCACAGAAA ACATCGCAGG TCAATGAATA CAAACTGAAG 
GAGCAGGAAC TTAAGGAGAA GGTTCACATC CTAGAAGACC AGGTGAAAAA CTTTACTCGT 
GGTGTATTTT ATACTCCTAT TGGCACACCG TATAAAGAAG TTAATAATCA ACATGCAGGG 
GTTTCTGTTT TTGGCGAACC CACTGAATTT GAGTACTTAA GAAAGGTGAT GTTTGAGTAC 
ATGATGGGGC GTGAAACAAA GACAATGGCA AAGGTAATAA CAACTGTGCT GAGGTTCCCA 
ACAGATCAAG CTCAACAAAT TTTGCAACGA GAAGAAACCC GACCACTGCC TCGCAGTGGT 
ATGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017950306.1
CDS	681..7049
Translation	MFKKLKQKISEEQSPSKSPLAPQQQGRPRSTSTGNRSRTSSVTEQQDDSLATADRENGSLSDNINGNDPTSSQRNEPQSFAHKLQMRVPSMESLFRSPIKESLFPSSTKENLLRSSSRDSLNKIDMENPSTVFDPPSDIESEAEDSVGSIEGLNKEQLFNRLHRMDKSLGNYRGKYSELVTAYRTMQRDKEKLQSILSQSQDKALRRIGELREELQMDQQAKKHLQEEFDASLEEKDQLISVLQTQVSLLKNRLQNGQSSTELPESTASPELDSESSLQEVDTDNRSTPDDGNAANTLNALQVRVKRQENLLQRCKKSIKSHKEQCAHLSSENEALQNQLDERLQELEKIKELHTSEKSKLITQLRDAKNSIEQLEQDKGMMIAETKRQMHETLELKEEEVSQLRSRVKKLSSHCEELQQQKEKLEKAAFEDVEKISSQKAEDAFEKLQMEEQIKAIEKASEEERVNLQKELSRVKQEVVDIMKKSCDSRISEIEKVHAEALLNKEQEYNEQIRLKEQEFQDQMNAVKKNEDETLNLLQDKEKSLMALEELERQKKTIESRAAEQLKEMQQEVENCRMRILDLESSVAEESKSQDFVSLIETERNNHNAEVATIKEMHKQDLDVLVQDQERIWSEKLELLKKENHTKIIELKEKYQRDYDSHLKEKEHNFQEHIEHMNEKTLEKLDVKQTELETLSAELSEAIKARQEIEQKLTDTENEISKIKLEYEAQIKEEREKHSEEIGSIEREKEMHTQGVEKDLKENINNLKLLQDSKECELEEFRKENQKLKEAAEKAEADHKEAFALLEAARHSHEINTHEKEQAYELKLKELQEMLKGMTQERNDLKEVTEKAETQLKNMQIELDSNKAQVQSLTKQIHEESMNMADKLNTLVLESETQVKGLKEQIEEISRIASEREHELKKVKEDHIQIILDLNNTISEKEKNILSLQEDYKVKHKNLDAKMEKLKQKFKELQESSKKKYSELESKLKKEIEKKQNELINKEREFNEKIHEMAQASSTGINHAMAQLELNHKEQIQNMTDNHQRELQETTEACEKKLSQLAEDLREKHEEELQEKDQVIAESRKQFNIKNKEKEDADKEISDLRELKTQMDATFNDLQEQLCQSAAQVLHLTQSENEFKTNCTRLTTEKQILQQQLDELKILAEEDKSKNDEIVNKVRLAEEKYQDLETLHNKVRADFAKELQDKLVEKENEFKHLLVKQENQLILCCKNAEDLFETLANGLTENHKYKLNGLIVKIANLESCMHKLKEVTLKQRSRIYEQEKQLEQFSNELLSVNQSLNQATQEKEFATLSLKNEIEALNFQNEMLKKEGGSQQQVADEKESCITQLKKELSENINIVTSLTEVVKQKESEVSSLSALINELKLKHENCVDLTEKENAIAFLTAQQKQSQQELQNQIQDLMAKIEVLNKEKSAQIDEAAKLKNKLDEWKKKAELKFVQNHNTIKELKEKVESVNKQIVEKDEKLRTVTEEFECHRSNIQETQLMSESKEFELTTQLEGQKSKISELEDQCSKLMSEKKSLMDQIEIQTIQQSEDKKALVEQLQHIQYASDNNSKEVQNKVITLEKEIDALKEKLDAEQTKWQLEKAECFQLKDKELKILEEKLTAESTNKLSELKKKAEQKIASLKKQLMSQIDEKEQSRKALESQLNVLKDNMQKESQEVQEGLQQKVENQDKKYALLLEQKKNTEADLANIRSELKLKELRVKEMEDILKDVQQKHSERELTDSEKEQALLKELESQMLTNNQLVSTHEKALRALQDDLNVKDAEIKTFKEEIDEKSKSLNELQVLFEETQSQESAMRIKLEEMESEKLKFKNEITKLQKDMRSLRKEHSQELDLLKKEITEEAEQRIKYEQEDSELKHNSKLKQLMREFNTQMAQKEREVETAVQETIAKAQEVEAELMRSQQIEAVHLHKKIAEKEDDLKRTVKRYEELLEAREEEMTAKVTELQEQLERLQEQLTQKEMQNEEEENVEEATSPEVFQAQLAQKTSQVNEYKLKEQELKEKVHILEDQVKNFTRGVFYTPIGTPYKEVNNQHAGVSVFGEPTEFEYLRKVMFEYMMGRETKTMAKVITTVLRFPTDQAQQILQREETRPLPRSGMF

Target ORF information:

RefSeq Version	XM_018094817.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis golgin A4 (golga4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094817.1

ORF Insert Sequence:

ATGTTTAAGA AACTGAAACA GAAGATCAGC GAAGAGCAGT CCCCTTCCAA AAGCCCCCTT 
GCTCCACAGC AGCAGGGTCG TCCAAGAAGC ACATCAACAG GCAACAGAAG TCGGACTTCG 
TCAGTAACAG AACAGCAGGA TGACAGCCTG GCAACAGCAG ATAGAGAGAA CGGATCATTG 
TCGGACAATA TTAATGGAAA TGATCCCACT TCTTCTCAGA GGAATGAACC TCAGTCTTTT 
GCACATAAGC TACAAATGCG GGTTCCTTCA ATGGAATCAC TGTTTCGAAG CCCCATTAAA 
GAATCACTTT TTCCTTCTTC AACTAAAGAA AATCTTTTGC GGAGTAGTTC ACGGGATTCC 
CTGAATAAAA TTGATATGGA GAATCCAAGT ACTGTATTTG ATCCCCCATC CGATATTGAA 
AGTGAAGCTG AGGATTCTGT GGGAAGTATA GAAGGACTTA ACAAAGAACA GCTGTTTAAT 
CGACTACATA GAATGGATAA AAGTTTAGGA AACTACAGAG GAAAATATTC TGAGCTTGTA 
ACAGCCTACA GAACAATGCA GAGAGACAAA GAGAAACTAC AGTCCATCTT GAGTCAGAGC 
CAGGACAAAG CACTAAGGAG AATTGGAGAG CTGCGAGAGG AGCTTCAAAT GGATCAACAG 
GCTAAAAAGC ATCTACAGGA AGAGTTTGAT GCATCTCTGG AAGAGAAAGA TCAACTTATC 
AGTGTTCTGC AAACACAGGT TAGTTTACTT AAAAACAGAC TTCAGAATGG ACAAAGCAGT 
ACTGAATTAC CAGAAAGCAC AGCCTCGCCA GAACTTGACT CTGAGAGTTC CTTGCAAGAG 
GTGGATACAG ATAACCGTTC CACCCCTGAT GATGGGAATG CTGCAAACAC TTTAAATGCT 
CTCCAGGTTA GAGTAAAACG TCAAGAGAAC CTTTTGCAAA GGTGTAAGAA ATCAATCAAG 
TCGCACAAGG AACAGTGTGC TCACCTCAGT AGTGAAAATG AAGCTCTTCA AAACCAACTA 
GATGAAAGAC TCCAGGAACT TGAGAAAATT AAAGAACTTC ACACTAGTGA GAAGAGCAAG 
CTGATTACCC AGCTTAGAGA TGCCAAGAAT TCAATTGAAC AGCTGGAACA AGATAAGGGT 
ATGATGATTG CAGAAACAAA ACGGCAGATG CATGAGACAT TAGAACTGAA GGAAGAAGAG 
GTTTCTCAGC TTCGGTCAAG GGTCAAGAAA TTAAGTTCAC ATTGTGAGGA ACTGCAGCAA 
CAGAAAGAAA AGTTAGAGAA AGCTGCATTT GAAGATGTAG AAAAAATATC TTCACAGAAA 
GCTGAGGATG CTTTTGAAAA GCTGCAGATG GAAGAGCAAA TTAAAGCTAT AGAAAAGGCC 
AGTGAGGAGG AGCGTGTCAA TCTTCAGAAG GAATTAAGTC GTGTGAAGCA AGAGGTGGTG 
GACATTATGA AGAAATCCTG TGATAGTCGT ATTAGTGAGA TTGAGAAAGT TCATGCAGAA 
GCCTTGCTGA ATAAGGAACA GGAGTATAAT GAACAGATTC GCCTGAAGGA ACAAGAATTC 
CAGGATCAAA TGAATGCTGT CAAAAAGAAT GAGGATGAGA CATTAAATTT GCTACAAGAT 
AAAGAAAAGA GTTTAATGGC ACTGGAAGAG CTCGAGCGAC AGAAAAAGAC TATTGAGTCC 
AGAGCAGCAG AACAACTTAA AGAAATGCAA CAAGAAGTTG AAAATTGCAG AATGAGGATT 
CTTGACCTTG AGAGTTCTGT TGCTGAAGAA AGTAAGTCCC AAGATTTTGT AAGCCTGATT 
GAAACAGAGA GAAATAATCA CAATGCAGAA GTAGCTACCA TCAAAGAAAT GCATAAGCAA 
GATCTTGATG TCCTTGTACA GGATCAAGAA AGGATATGGT CAGAAAAGCT TGAGCTGCTT 
AAGAAAGAGA ATCATACTAA GATTATTGAA CTTAAAGAGA AGTATCAGCG AGATTATGAT 
TCTCACTTGA AAGAGAAAGA GCATAATTTC CAGGAGCATA TTGAGCATAT GAATGAAAAA 
ACATTAGAAA AACTAGATGT AAAACAAACT GAATTGGAAA CCCTCTCAGC TGAACTGTCT 
GAGGCTATAA AGGCACGGCA AGAGATTGAG CAGAAACTTA CAGACACAGA AAATGAGATT 
AGCAAAATAA AACTAGAGTA TGAAGCCCAG ATAAAAGAAG AAAGAGAAAA ACACAGTGAA 
GAAATTGGTT CCATTGAAAG AGAGAAGGAA ATGCATACTC AGGGTGTTGA GAAAGACTTG 
AAGGAAAATA TTAACAACCT AAAACTACTT CAAGATAGTA AAGAATGTGA GTTGGAAGAA 
TTCAGAAAGG AAAATCAAAA GCTAAAAGAA GCTGCTGAAA AGGCAGAAGC AGACCATAAA 
GAAGCATTTG CTCTATTGGA AGCAGCCAGG CATTCTCATG AGATAAATAC ACATGAGAAA 
GAACAGGCTT ATGAGCTAAA ATTGAAGGAG CTTCAGGAGA TGCTAAAAGG TATGACGCAG 
GAGCGAAACG ATCTTAAAGA GGTGACAGAA AAAGCAGAAA CACAGTTAAA GAATATGCAG 
ATCGAGCTGG ATTCTAACAA GGCCCAAGTA CAAAGCCTTA CAAAGCAAAT TCATGAGGAA 
AGTATGAATA TGGCAGATAA ACTTAACACT TTGGTACTAG AGTCTGAAAC TCAGGTAAAA 
GGGTTGAAGG AACAAATAGA AGAGATAAGC AGAATAGCTT CAGAGCGTGA ACATGAACTT 
AAAAAAGTAA AAGAAGATCA TATTCAAATT ATTTTAGACC TAAACAATAC TATATCTGAA 
AAAGAGAAGA ATATACTTTC TTTGCAAGAA GATTACAAAG TAAAACACAA AAATCTGGAT 
GCTAAAATGG AAAAATTAAA GCAGAAGTTT AAAGAGCTGC AAGAATCATC TAAAAAGAAA 
TATTCAGAGT TGGAAAGCAA ACTTAAGAAA GAGATCGAGA AGAAGCAGAA CGAGTTGATT 
AACAAAGAAA GGGAGTTTAA TGAAAAAATA CATGAGATGG CCCAAGCAAG TTCAACTGGA 
ATTAATCATG CAATGGCTCA GCTTGAACTG AATCATAAAG AGCAGATTCA GAATATGACA 
GACAATCATC AGAGAGAATT ACAGGAAACA ACAGAAGCTT GTGAAAAGAA ATTAAGCCAA 
CTTGCTGAAG ACCTTAGAGA GAAGCACGAA GAAGAACTTC AGGAGAAAGA TCAGGTCATA 
GCAGAATCAA GAAAACAGTT TAATATCAAG AATAAAGAAA AAGAAGATGC AGACAAAGAG 
ATATCAGACC TCAGGGAACT GAAGACCCAA ATGGATGCCA CGTTTAATGA TTTGCAAGAA 
CAGCTGTGTC AGTCAGCAGC TCAAGTTTTA CATTTGACAC AGAGTGAAAA TGAATTCAAG 
ACAAATTGTA CTCGGCTAAC AACGGAGAAG CAAATTCTTC AACAACAGCT TGATGAACTG 
AAAATATTAG CAGAGGAAGA TAAAAGCAAG AATGATGAAA TAGTTAACAA GGTGAGACTT 
GCTGAAGAAA AGTATCAAGA CTTGGAGACC CTGCACAATA AAGTGAGAGC AGACTTTGCT 
AAGGAACTGC AGGATAAACT TGTTGAAAAG GAAAATGAGT TTAAACACCT TTTGGTTAAA 
CAAGAAAACC AGTTGATCTT GTGCTGCAAA AATGCTGAAG ATTTATTTGA AACACTTGCA 
AATGGACTCA CTGAAAACCA CAAATATAAG TTGAATGGAT TGATTGTTAA AATAGCAAAT 
CTTGAAAGCT GCATGCATAA ACTAAAAGAG GTCACTTTAA AACAGCGTAG TAGAATATAT 
GAACAAGAAA AACAACTTGA GCAGTTTTCA AACGAACTTC TTTCAGTAAA CCAATCTCTT 
AATCAAGCAA CTCAGGAGAA AGAATTTGCT ACTTTGTCGC TAAAGAATGA GATTGAAGCT 
CTTAATTTTC AAAATGAGAT GTTAAAAAAA GAAGGAGGAA GTCAACAGCA GGTTGCAGAT 
GAGAAAGAAT CTTGCATTAC TCAGCTGAAA AAAGAACTTT CAGAGAATAT AAATATTGTG 
ACTTCCTTGA CAGAAGTGGT AAAACAGAAA GAATCTGAGG TATCTTCTCT TTCTGCCTTA 
ATTAATGAAC TTAAACTGAA ACATGAGAAT TGTGTAGATT TAACAGAAAA AGAAAATGCA 
ATTGCTTTTC TCACTGCACA GCAGAAACAG AGTCAGCAAG AGCTACAGAA TCAAATTCAG 
GATCTAATGG CAAAGATTGA GGTTCTGAAT AAAGAAAAAT CTGCACAAAT AGATGAGGCT 
GCTAAACTTA AAAATAAGTT GGATGAATGG AAGAAAAAGG CAGAACTTAA ATTTGTGCAG 
AACCACAACA CTATTAAAGA GCTAAAAGAG AAAGTTGAAT CAGTCAATAA GCAAATTGTT 
GAAAAAGATG AAAAACTTAG GACAGTGACA GAAGAATTTG AATGTCATCG TAGCAATATC 
CAAGAAACAC AACTCATGAG TGAAAGCAAA GAATTTGAAT TAACTACCCA GTTGGAAGGC 
CAGAAATCTA AAATTTCGGA ATTGGAGGAC CAGTGTTCGA AGCTGATGTC TGAAAAGAAA 
AGTTTGATGG ATCAAATCGA AATTCAAACT ATTCAGCAGA GTGAAGATAA GAAAGCACTG 
GTTGAGCAAC TCCAGCATAT TCAGTATGCT TCTGACAATA ACTCCAAGGA AGTGCAAAAT 
AAAGTTATAA CACTTGAAAA AGAAATTGAT GCTTTAAAGG AGAAACTTGA TGCAGAGCAA 
ACAAAATGGC AGCTGGAGAA AGCAGAGTGC TTTCAGCTGA AAGATAAGGA ATTAAAAATA 
TTGGAAGAGA AACTAACTGC AGAAAGCACA AATAAACTTT CAGAGCTCAA GAAAAAAGCT 
GAACAGAAAA TCGCTTCTCT TAAGAAACAA CTTATGTCTC AGATAGATGA GAAGGAGCAG 
TCCAGGAAAG CGCTGGAAAG CCAGCTAAAT GTCTTAAAAG ATAATATGCA GAAAGAGAGC 
CAGGAAGTGC AGGAAGGATT GCAACAAAAA GTTGAAAATC AAGACAAAAA ATATGCATTG 
CTACTGGAGC AGAAGAAAAA TACAGAAGCA GATTTAGCTA ATATTAGAAG TGAACTAAAA 
TTAAAGGAAC TAAGAGTTAA AGAAATGGAA GATATTCTTA AAGATGTCCA GCAAAAACAT 
TCTGAAAGAG AGCTGACCGA CAGTGAGAAA GAACAAGCAC TTTTAAAAGA ACTAGAGTCA 
CAGATGCTGA CAAATAATCA GCTGGTTTCT ACTCATGAAA AAGCTTTAAG AGCCCTGCAA 
GATGACCTCA ATGTCAAGGA TGCAGAGATA AAGACCTTTA AAGAGGAAAT AGATGAGAAA 
TCCAAATCCT TAAATGAACT CCAAGTGCTT TTTGAGGAGA CTCAGTCTCA AGAGAGCGCA 
ATGAGAATAA AACTTGAGGA AATGGAGAGT GAGAAGCTAA AATTCAAGAA TGAAATAACT 
AAGCTACAGA AAGATATGCG CTCTTTACGT AAGGAACACA GCCAAGAACT AGATTTGCTT 
AAAAAGGAGA TAACCGAGGA AGCCGAACAA AGAATCAAAT ACGAACAGGA AGACTCGGAA 
CTTAAGCATA ATTCTAAATT AAAGCAACTC ATGAGAGAAT TCAATACCCA GATGGCTCAA 
AAGGAGCGTG AGGTTGAAAC AGCTGTTCAG GAGACTATTG CTAAAGCTCA GGAGGTTGAG 
GCTGAATTAA TGCGATCACA ACAGATAGAA GCAGTCCATC TTCATAAAAA GATTGCTGAG 
AAAGAAGATG ACCTAAAGAG AACTGTAAAA AGATATGAAG AACTTTTAGA GGCCCGAGAA 
GAGGAAATGA CAGCAAAAGT GACAGAGTTA CAAGAGCAAC TTGAAAGATT ACAGGAACAA 
TTAACTCAGA AGGAAATGCA AAATGAAGAA GAAGAAAATG TGGAGGAGGC AACATCTCCT 
GAGGTTTTTC AGGCGCAGCT GGCACAGAAA ACATCGCAGG TCAATGAATA CAAACTGAAG 
GAGCAGGAAC TTAAGGAGAA GGTTCACATC CTAGAAGACC AGGTGAAAAA CTTTACTCGT 
GGTGTATTTT ATACTCCTAT TGGCACACCG TATAAAGAAG TTAATAATCA ACATGCAGGG 
GTTTCTGTTT TTGGCGAACC CACTGAATTT GAGTACTTAA GAAAGGTGAT GTTTGAGTAC 
ATGATGGGGC GTGAAACAAA GACAATGGCA AAGGTAATAA CAACTGTGCT GAGGTTCCCA 
ACAGATCAAG CTCAACAAAT TTTGCAACGA GAAGAAACCC GACCACTGCC TCGCAGTGGT 
ATGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

golga4 ( XM_018094817.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017950306.1 Xenopus tropicalis golgin A4 (golga4), transcript variant X2, mRNA.

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CloneID	OXa45017
Clone ID Related Accession (Same CDS sequence)	XM_018094817.1 , XM_018094817.2
Accession Version	XM_018094817.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	golgin subfamily A member 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018094817.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAAGA AACTGAAACA GAAGATCAGC GAAGAGCAGT CCCCTTCCAA AAGCCCCCTT 
GCTCCACAGC AGCAGGGTCG TCCAAGAAGC ACATCAACAG GCAACAGAAG TCGGACTTCG 
TCAGTAACAG AACAGCAGGA TGACAGCCTG GCAACAGCAG ATAGAGAGAA CGGATCATTG 
TCGGACAATA TTAATGGAAA TGATCCCACT TCTTCTCAGA GGAATGAACC TCAGTCTTTT 
GCACATAAGC TACAAATGCG GGTTCCTTCA ATGGAATCAC TGTTTCGAAG CCCCATTAAA 
GAATCACTTT TTCCTTCTTC AACTAAAGAA AATCTTTTGC GGAGTAGTTC ACGGGATTCC 
CTGAATAAAA TTGATATGGA GAATCCAAGT ACTGTATTTG ATCCCCCATC CGATATTGAA 
AGTGAAGCTG AGGATTCTGT GGGAAGTATA GAAGGACTTA ACAAAGAACA GCTGTTTAAT 
CGACTACATA GAATGGATAA AAGTTTAGGA AACTACAGAG GAAAATATTC TGAGCTTGTA 
ACAGCCTACA GAACAATGCA GAGAGACAAA GAGAAACTAC AGTCCATCTT GAGTCAGAGC 
CAGGACAAAG CACTAAGGAG AATTGGAGAG CTGCGAGAGG AGCTTCAAAT GGATCAACAG 
GCTAAAAAGC ATCTACAGGA AGAGTTTGAT GCATCTCTGG AAGAGAAAGA TCAACTTATC 
AGTGTTCTGC AAACACAGGT TAGTTTACTT AAAAACAGAC TTCAGAATGG ACAAAGCAGT 
ACTGAATTAC CAGAAAGCAC AGCCTCGCCA GAACTTGACT CTGAGAGTTC CTTGCAAGAG 
GTGGATACAG ATAACCGTTC CACCCCTGAT GATGGGAATG CTGCAAACAC TTTAAATGCT 
CTCCAGGTTA GAGTAAAACG TCAAGAGAAC CTTTTGCAAA GGTGTAAGAA ATCAATCAAG 
TCGCACAAGG AACAGTGTGC TCACCTCAGT AGTGAAAATG AAGCTCTTCA AAACCAACTA 
GATGAAAGAC TCCAGGAACT TGAGAAAATT AAAGAACTTC ACACTAGTGA GAAGAGCAAG 
CTGATTACCC AGCTTAGAGA TGCCAAGAAT TCAATTGAAC AGCTGGAACA AGATAAGGGT 
ATGATGATTG CAGAAACAAA ACGGCAGATG CATGAGACAT TAGAACTGAA GGAAGAAGAG 
GTTTCTCAGC TTCGGTCAAG GGTCAAGAAA TTAAGTTCAC ATTGTGAGGA ACTGCAGCAA 
CAGAAAGAAA AGTTAGAGAA AGCTGCATTT GAAGATGTAG AAAAAATATC TTCACAGAAA 
GCTGAGGATG CTTTTGAAAA GCTGCAGATG GAAGAGCAAA TTAAAGCTAT AGAAAAGGCC 
AGTGAGGAGG AGCGTGTCAA TCTTCAGAAG GAATTAAGTC GTGTGAAGCA AGAGGTGGTG 
GACATTATGA AGAAATCCTG TGATAGTCGT ATTAGTGAGA TTGAGAAAGT TCATGCAGAA 
GCCTTGCTGA ATAAGGAACA GGAGTATAAT GAACAGATTC GCCTGAAGGA ACAAGAATTC 
CAGGATCAAA TGAATGCTGT CAAAAAGAAT GAGGATGAGA CATTAAATTT GCTACAAGAT 
AAAGAAAAGA GTTTAATGGC ACTGGAAGAG CTCGAGCGAC AGAAAAAGAC TATTGAGTCC 
AGAGCAGCAG AACAACTTAA AGAAATGCAA CAAGAAGTTG AAAATTGCAG AATGAGGATT 
CTTGACCTTG AGAGTTCTGT TGCTGAAGAA AGTAAGTCCC AAGATTTTGT AAGCCTGATT 
GAAACAGAGA GAAATAATCA CAATGCAGAA GTAGCTACCA TCAAAGAAAT GCATAAGCAA 
GATCTTGATG TCCTTGTACA GGATCAAGAA AGGATATGGT CAGAAAAGCT TGAGCTGCTT 
AAGAAAGAGA ATCATACTAA GATTATTGAA CTTAAAGAGA AGTATCAGCG AGATTATGAT 
TCTCACTTGA AAGAGAAAGA GCATAATTTC CAGGAGCATA TTGAGCATAT GAATGAAAAA 
ACATTAGAAA AACTAGATGT AAAACAAACT GAATTGGAAA CCCTCTCAGC TGAACTGTCT 
GAGGCTATAA AGGCACGGCA AGAGATTGAG CAGAAACTTA CAGACACAGA AAATGAGATT 
AGCAAAATAA AACTAGAGTA TGAAGCCCAG ATAAAAGAAG AAAGAGAAAA ACACAGTGAA 
GAAATTGGTT CCATTGAAAG AGAGAAGGAA ATGCATACTC AGGGTGTTGA GAAAGACTTG 
AAGGAAAATA TTAACAACCT AAAACTACTT CAAGATAGTA AAGAATGTGA GTTGGAAGAA 
TTCAGAAAGG AAAATCAAAA GCTAAAAGAA GCTGCTGAAA AGGCAGAAGC AGACCATAAA 
GAAGCATTTG CTCTATTGGA AGCAGCCAGG CATTCTCATG AGATAAATAC ACATGAGAAA 
GAACAGGCTT ATGAGCTAAA ATTGAAGGAG CTTCAGGAGA TGCTAAAAGG TATGACGCAG 
GAGCGAAACG ATCTTAAAGA GGTGACAGAA AAAGCAGAAA CACAGTTAAA GAATATGCAG 
ATCGAGCTGG ATTCTAACAA GGCCCAAGTA CAAAGCCTTA CAAAGCAAAT TCATGAGGAA 
AGTATGAATA TGGCAGATAA ACTTAACACT TTGGTACTAG AGTCTGAAAC TCAGGTAAAA 
GGGTTGAAGG AACAAATAGA AGAGATAAGC AGAATAGCTT CAGAGCGTGA ACATGAACTT 
AAAAAAGTAA AAGAAGATCA TATTCAAATT ATTTTAGACC TAAACAATAC TATATCTGAA 
AAAGAGAAGA ATATACTTTC TTTGCAAGAA GATTACAAAG TAAAACACAA AAATCTGGAT 
GCTAAAATGG AAAAATTAAA GCAGAAGTTT AAAGAGCTGC AAGAATCATC TAAAAAGAAA 
TATTCAGAGT TGGAAAGCAA ACTTAAGAAA GAGATCGAGA AGAAGCAGAA CGAGTTGATT 
AACAAAGAAA GGGAGTTTAA TGAAAAAATA CATGAGATGG CCCAAGCAAG TTCAACTGGA 
ATTAATCATG CAATGGCTCA GCTTGAACTG AATCATAAAG AGCAGATTCA GAATATGACA 
GACAATCATC AGAGAGAATT ACAGGAAACA ACAGAAGCTT GTGAAAAGAA ATTAAGCCAA 
CTTGCTGAAG ACCTTAGAGA GAAGCACGAA GAAGAACTTC AGGAGAAAGA TCAGGTCATA 
GCAGAATCAA GAAAACAGTT TAATATCAAG AATAAAGAAA AAGAAGATGC AGACAAAGAG 
ATATCAGACC TCAGGGAACT GAAGACCCAA ATGGATGCCA CGTTTAATGA TTTGCAAGAA 
CAGCTGTGTC AGTCAGCAGC TCAAGTTTTA CATTTGACAC AGAGTGAAAA TGAATTCAAG 
ACAAATTGTA CTCGGCTAAC AACGGAGAAG CAAATTCTTC AACAACAGCT TGATGAACTG 
AAAATATTAG CAGAGGAAGA TAAAAGCAAG AATGATGAAA TAGTTAACAA GGTGAGACTT 
GCTGAAGAAA AGTATCAAGA CTTGGAGACC CTGCACAATA AAGTGAGAGC AGACTTTGCT 
AAGGAACTGC AGGATAAACT TGTTGAAAAG GAAAATGAGT TTAAACACCT TTTGGTTAAA 
CAAGAAAACC AGTTGATCTT GTGCTGCAAA AATGCTGAAG ATTTATTTGA AACACTTGCA 
AATGGACTCA CTGAAAACCA CAAATATAAG TTGAATGGAT TGATTGTTAA AATAGCAAAT 
CTTGAAAGCT GCATGCATAA ACTAAAAGAG GTCACTTTAA AACAGCGTAG TAGAATATAT 
GAACAAGAAA AACAACTTGA GCAGTTTTCA AACGAACTTC TTTCAGTAAA CCAATCTCTT 
AATCAAGCAA CTCAGGAGAA AGAATTTGCT ACTTTGTCGC TAAAGAATGA GATTGAAGCT 
CTTAATTTTC AAAATGAGAT GTTAAAAAAA GAAGGAGGAA GTCAACAGCA GGTTGCAGAT 
GAGAAAGAAT CTTGCATTAC TCAGCTGAAA AAAGAACTTT CAGAGAATAT AAATATTGTG 
ACTTCCTTGA CAGAAGTGGT AAAACAGAAA GAATCTGAGG TATCTTCTCT TTCTGCCTTA 
ATTAATGAAC TTAAACTGAA ACATGAGAAT TGTGTAGATT TAACAGAAAA AGAAAATGCA 
ATTGCTTTTC TCACTGCACA GCAGAAACAG AGTCAGCAAG AGCTACAGAA TCAAATTCAG 
GATCTAATGG CAAAGATTGA GGTTCTGAAT AAAGAAAAAT CTGCACAAAT AGATGAGGCT 
GCTAAACTTA AAAATAAGTT GGATGAATGG AAGAAAAAGG CAGAACTTAA ATTTGTGCAG 
AACCACAACA CTATTAAAGA GCTAAAAGAG AAAGTTGAAT CAGTCAATAA GCAAATTGTT 
GAAAAAGATG AAAAACTTAG GACAGTGACA GAAGAATTTG AATGTCATCG TAGCAATATC 
CAAGAAACAC AACTCATGAG TGAAAGCAAA GAATTTGAAT TAACTACCCA GTTGGAAGGC 
CAGAAATCTA AAATTTCGGA ATTGGAGGAC CAGTGTTCGA AGCTGATGTC TGAAAAGAAA 
AGTTTGATGG ATCAAATCGA AATTCAAACT ATTCAGCAGA GTGAAGATAA GAAAGCACTG 
GTTGAGCAAC TCCAGCATAT TCAGTATGCT TCTGACAATA ACTCCAAGGA AGTGCAAAAT 
AAAGTTATAA CACTTGAAAA AGAAATTGAT GCTTTAAAGG AGAAACTTGA TGCAGAGCAA 
ACAAAATGGC AGCTGGAGAA AGCAGAGTGC TTTCAGCTGA AAGATAAGGA ATTAAAAATA 
TTGGAAGAGA AACTAACTGC AGAAAGCACA AATAAACTTT CAGAGCTCAA GAAAAAAGCT 
GAACAGAAAA TCGCTTCTCT TAAGAAACAA CTTATGTCTC AGATAGATGA GAAGGAGCAG 
TCCAGGAAAG CGCTGGAAAG CCAGCTAAAT GTCTTAAAAG ATAATATGCA GAAAGAGAGC 
CAGGAAGTGC AGGAAGGATT GCAACAAAAA GTTGAAAATC AAGACAAAAA ATATGCATTG 
CTACTGGAGC AGAAGAAAAA TACAGAAGCA GATTTAGCTA ATATTAGAAG TGAACTAAAA 
TTAAAGGAAC TAAGAGTTAA AGAAATGGAA GATATTCTTA AAGATGTCCA GCAAAAACAT 
TCTGAAAGAG AGCTGACCGA CAGTGAGAAA GAACAAGCAC TTTTAAAAGA ACTAGAGTCA 
CAGATGCTGA CAAATAATCA GCTGGTTTCT ACTCATGAAA AAGCTTTAAG AGCCCTGCAA 
GATGACCTCA ATGTCAAGGA TGCAGAGATA AAGACCTTTA AAGAGGAAAT AGATGAGAAA 
TCCAAATCCT TAAATGAACT CCAAGTGCTT TTTGAGGAGA CTCAGTCTCA AGAGAGCGCA 
ATGAGAATAA AACTTGAGGA AATGGAGAGT GAGAAGCTAA AATTCAAGAA TGAAATAACT 
AAGCTACAGA AAGATATGCG CTCTTTACGT AAGGAACACA GCCAAGAACT AGATTTGCTT 
AAAAAGGAGA TAACCGAGGA AGCCGAACAA AGAATCAAAT ACGAACAGGA AGACTCGGAA 
CTTAAGCATA ATTCTAAATT AAAGCAACTC ATGAGAGAAT TCAATACCCA GATGGCTCAA 
AAGGAGCGTG AGGTTGAAAC AGCTGTTCAG GAGACTATTG CTAAAGCTCA GGAGGTTGAG 
GCTGAATTAA TGCGATCACA ACAGATAGAA GCAGTCCATC TTCATAAAAA GATTGCTGAG 
AAAGAAGATG ACCTAAAGAG AACTGTAAAA AGATATGAAG AACTTTTAGA GGCCCGAGAA 
GAGGAAATGA CAGCAAAAGT GACAGAGTTA CAAGAGCAAC TTGAAAGATT ACAGGAACAA 
TTAACTCAGA AGGAAATGCA AAATGAAGAA GAAGAAAATG TGGAGGAGGC AACATCTCCT 
GAGGTTTTTC AGGCGCAGCT GGCACAGAAA ACATCGCAGG TCAATGAATA CAAACTGAAG 
GAGCAGGAAC TTAAGGAGAA GGTTCACATC CTAGAAGACC AGGTGAAAAA CTTTACTCGT 
GGTGTATTTT ATACTCCTAT TGGCACACCG TATAAAGAAG TTAATAATCA ACATGCAGGG 
GTTTCTGTTT TTGGCGAACC CACTGAATTT GAGTACTTAA GAAAGGTGAT GTTTGAGTAC 
ATGATGGGGC GTGAAACAAA GACAATGGCA AAGGTAATAA CAACTGTGCT GAGGTTCCCA 
ACAGATCAAG CTCAACAAAT TTTGCAACGA GAAGAAACCC GACCACTGCC TCGCAGTGGT 
ATGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017950306.1
CDS	219..6587
Translation	MFKKLKQKISEEQSPSKSPLAPQQQGRPRSTSTGNRSRTSSVTEQQDDSLATADRENGSLSDNINGNDPTSSQRNEPQSFAHKLQMRVPSMESLFRSPIKESLFPSSTKENLLRSSSRDSLNKIDMENPSTVFDPPSDIESEAEDSVGSIEGLNKEQLFNRLHRMDKSLGNYRGKYSELVTAYRTMQRDKEKLQSILSQSQDKALRRIGELREELQMDQQAKKHLQEEFDASLEEKDQLISVLQTQVSLLKNRLQNGQSSTELPESTASPELDSESSLQEVDTDNRSTPDDGNAANTLNALQVRVKRQENLLQRCKKSIKSHKEQCAHLSSENEALQNQLDERLQELEKIKELHTSEKSKLITQLRDAKNSIEQLEQDKGMMIAETKRQMHETLELKEEEVSQLRSRVKKLSSHCEELQQQKEKLEKAAFEDVEKISSQKAEDAFEKLQMEEQIKAIEKASEEERVNLQKELSRVKQEVVDIMKKSCDSRISEIEKVHAEALLNKEQEYNEQIRLKEQEFQDQMNAVKKNEDETLNLLQDKEKSLMALEELERQKKTIESRAAEQLKEMQQEVENCRMRILDLESSVAEESKSQDFVSLIETERNNHNAEVATIKEMHKQDLDVLVQDQERIWSEKLELLKKENHTKIIELKEKYQRDYDSHLKEKEHNFQEHIEHMNEKTLEKLDVKQTELETLSAELSEAIKARQEIEQKLTDTENEISKIKLEYEAQIKEEREKHSEEIGSIEREKEMHTQGVEKDLKENINNLKLLQDSKECELEEFRKENQKLKEAAEKAEADHKEAFALLEAARHSHEINTHEKEQAYELKLKELQEMLKGMTQERNDLKEVTEKAETQLKNMQIELDSNKAQVQSLTKQIHEESMNMADKLNTLVLESETQVKGLKEQIEEISRIASEREHELKKVKEDHIQIILDLNNTISEKEKNILSLQEDYKVKHKNLDAKMEKLKQKFKELQESSKKKYSELESKLKKEIEKKQNELINKEREFNEKIHEMAQASSTGINHAMAQLELNHKEQIQNMTDNHQRELQETTEACEKKLSQLAEDLREKHEEELQEKDQVIAESRKQFNIKNKEKEDADKEISDLRELKTQMDATFNDLQEQLCQSAAQVLHLTQSENEFKTNCTRLTTEKQILQQQLDELKILAEEDKSKNDEIVNKVRLAEEKYQDLETLHNKVRADFAKELQDKLVEKENEFKHLLVKQENQLILCCKNAEDLFETLANGLTENHKYKLNGLIVKIANLESCMHKLKEVTLKQRSRIYEQEKQLEQFSNELLSVNQSLNQATQEKEFATLSLKNEIEALNFQNEMLKKEGGSQQQVADEKESCITQLKKELSENINIVTSLTEVVKQKESEVSSLSALINELKLKHENCVDLTEKENAIAFLTAQQKQSQQELQNQIQDLMAKIEVLNKEKSAQIDEAAKLKNKLDEWKKKAELKFVQNHNTIKELKEKVESVNKQIVEKDEKLRTVTEEFECHRSNIQETQLMSESKEFELTTQLEGQKSKISELEDQCSKLMSEKKSLMDQIEIQTIQQSEDKKALVEQLQHIQYASDNNSKEVQNKVITLEKEIDALKEKLDAEQTKWQLEKAECFQLKDKELKILEEKLTAESTNKLSELKKKAEQKIASLKKQLMSQIDEKEQSRKALESQLNVLKDNMQKESQEVQEGLQQKVENQDKKYALLLEQKKNTEADLANIRSELKLKELRVKEMEDILKDVQQKHSERELTDSEKEQALLKELESQMLTNNQLVSTHEKALRALQDDLNVKDAEIKTFKEEIDEKSKSLNELQVLFEETQSQESAMRIKLEEMESEKLKFKNEITKLQKDMRSLRKEHSQELDLLKKEITEEAEQRIKYEQEDSELKHNSKLKQLMREFNTQMAQKEREVETAVQETIAKAQEVEAELMRSQQIEAVHLHKKIAEKEDDLKRTVKRYEELLEAREEEMTAKVTELQEQLERLQEQLTQKEMQNEEEENVEEATSPEVFQAQLAQKTSQVNEYKLKEQELKEKVHILEDQVKNFTRGVFYTPIGTPYKEVNNQHAGVSVFGEPTEFEYLRKVMFEYMMGRETKTMAKVITTVLRFPTDQAQQILQREETRPLPRSGMF

Target ORF information:

RefSeq Version	XM_018094817.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis golgin A4 (golga4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094817.2

ORF Insert Sequence:

ATGTTTAAGA AACTGAAACA GAAGATCAGC GAAGAGCAGT CCCCTTCCAA AAGCCCCCTT 
GCTCCACAGC AGCAGGGTCG TCCAAGAAGC ACATCAACAG GCAACAGAAG TCGGACTTCG 
TCAGTAACAG AACAGCAGGA TGACAGCCTG GCAACAGCAG ATAGAGAGAA CGGATCATTG 
TCGGACAATA TTAATGGAAA TGATCCCACT TCTTCTCAGA GGAATGAACC TCAGTCTTTT 
GCACATAAGC TACAAATGCG GGTTCCTTCA ATGGAATCAC TGTTTCGAAG CCCCATTAAA 
GAATCACTTT TTCCTTCTTC AACTAAAGAA AATCTTTTGC GGAGTAGTTC ACGGGATTCC 
CTGAATAAAA TTGATATGGA GAATCCAAGT ACTGTATTTG ATCCCCCATC CGATATTGAA 
AGTGAAGCTG AGGATTCTGT GGGAAGTATA GAAGGACTTA ACAAAGAACA GCTGTTTAAT 
CGACTACATA GAATGGATAA AAGTTTAGGA AACTACAGAG GAAAATATTC TGAGCTTGTA 
ACAGCCTACA GAACAATGCA GAGAGACAAA GAGAAACTAC AGTCCATCTT GAGTCAGAGC 
CAGGACAAAG CACTAAGGAG AATTGGAGAG CTGCGAGAGG AGCTTCAAAT GGATCAACAG 
GCTAAAAAGC ATCTACAGGA AGAGTTTGAT GCATCTCTGG AAGAGAAAGA TCAACTTATC 
AGTGTTCTGC AAACACAGGT TAGTTTACTT AAAAACAGAC TTCAGAATGG ACAAAGCAGT 
ACTGAATTAC CAGAAAGCAC AGCCTCGCCA GAACTTGACT CTGAGAGTTC CTTGCAAGAG 
GTGGATACAG ATAACCGTTC CACCCCTGAT GATGGGAATG CTGCAAACAC TTTAAATGCT 
CTCCAGGTTA GAGTAAAACG TCAAGAGAAC CTTTTGCAAA GGTGTAAGAA ATCAATCAAG 
TCGCACAAGG AACAGTGTGC TCACCTCAGT AGTGAAAATG AAGCTCTTCA AAACCAACTA 
GATGAAAGAC TCCAGGAACT TGAGAAAATT AAAGAACTTC ACACTAGTGA GAAGAGCAAG 
CTGATTACCC AGCTTAGAGA TGCCAAGAAT TCAATTGAAC AGCTGGAACA AGATAAGGGT 
ATGATGATTG CAGAAACAAA ACGGCAGATG CATGAGACAT TAGAACTGAA GGAAGAAGAG 
GTTTCTCAGC TTCGGTCAAG GGTCAAGAAA TTAAGTTCAC ATTGTGAGGA ACTGCAGCAA 
CAGAAAGAAA AGTTAGAGAA AGCTGCATTT GAAGATGTAG AAAAAATATC TTCACAGAAA 
GCTGAGGATG CTTTTGAAAA GCTGCAGATG GAAGAGCAAA TTAAAGCTAT AGAAAAGGCC 
AGTGAGGAGG AGCGTGTCAA TCTTCAGAAG GAATTAAGTC GTGTGAAGCA AGAGGTGGTG 
GACATTATGA AGAAATCCTG TGATAGTCGT ATTAGTGAGA TTGAGAAAGT TCATGCAGAA 
GCCTTGCTGA ATAAGGAACA GGAGTATAAT GAACAGATTC GCCTGAAGGA ACAAGAATTC 
CAGGATCAAA TGAATGCTGT CAAAAAGAAT GAGGATGAGA CATTAAATTT GCTACAAGAT 
AAAGAAAAGA GTTTAATGGC ACTGGAAGAG CTCGAGCGAC AGAAAAAGAC TATTGAGTCC 
AGAGCAGCAG AACAACTTAA AGAAATGCAA CAAGAAGTTG AAAATTGCAG AATGAGGATT 
CTTGACCTTG AGAGTTCTGT TGCTGAAGAA AGTAAGTCCC AAGATTTTGT AAGCCTGATT 
GAAACAGAGA GAAATAATCA CAATGCAGAA GTAGCTACCA TCAAAGAAAT GCATAAGCAA 
GATCTTGATG TCCTTGTACA GGATCAAGAA AGGATATGGT CAGAAAAGCT TGAGCTGCTT 
AAGAAAGAGA ATCATACTAA GATTATTGAA CTTAAAGAGA AGTATCAGCG AGATTATGAT 
TCTCACTTGA AAGAGAAAGA GCATAATTTC CAGGAGCATA TTGAGCATAT GAATGAAAAA 
ACATTAGAAA AACTAGATGT AAAACAAACT GAATTGGAAA CCCTCTCAGC TGAACTGTCT 
GAGGCTATAA AGGCACGGCA AGAGATTGAG CAGAAACTTA CAGACACAGA AAATGAGATT 
AGCAAAATAA AACTAGAGTA TGAAGCCCAG ATAAAAGAAG AAAGAGAAAA ACACAGTGAA 
GAAATTGGTT CCATTGAAAG AGAGAAGGAA ATGCATACTC AGGGTGTTGA GAAAGACTTG 
AAGGAAAATA TTAACAACCT AAAACTACTT CAAGATAGTA AAGAATGTGA GTTGGAAGAA 
TTCAGAAAGG AAAATCAAAA GCTAAAAGAA GCTGCTGAAA AGGCAGAAGC AGACCATAAA 
GAAGCATTTG CTCTATTGGA AGCAGCCAGG CATTCTCATG AGATAAATAC ACATGAGAAA 
GAACAGGCTT ATGAGCTAAA ATTGAAGGAG CTTCAGGAGA TGCTAAAAGG TATGACGCAG 
GAGCGAAACG ATCTTAAAGA GGTGACAGAA AAAGCAGAAA CACAGTTAAA GAATATGCAG 
ATCGAGCTGG ATTCTAACAA GGCCCAAGTA CAAAGCCTTA CAAAGCAAAT TCATGAGGAA 
AGTATGAATA TGGCAGATAA ACTTAACACT TTGGTACTAG AGTCTGAAAC TCAGGTAAAA 
GGGTTGAAGG AACAAATAGA AGAGATAAGC AGAATAGCTT CAGAGCGTGA ACATGAACTT 
AAAAAAGTAA AAGAAGATCA TATTCAAATT ATTTTAGACC TAAACAATAC TATATCTGAA 
AAAGAGAAGA ATATACTTTC TTTGCAAGAA GATTACAAAG TAAAACACAA AAATCTGGAT 
GCTAAAATGG AAAAATTAAA GCAGAAGTTT AAAGAGCTGC AAGAATCATC TAAAAAGAAA 
TATTCAGAGT TGGAAAGCAA ACTTAAGAAA GAGATCGAGA AGAAGCAGAA CGAGTTGATT 
AACAAAGAAA GGGAGTTTAA TGAAAAAATA CATGAGATGG CCCAAGCAAG TTCAACTGGA 
ATTAATCATG CAATGGCTCA GCTTGAACTG AATCATAAAG AGCAGATTCA GAATATGACA 
GACAATCATC AGAGAGAATT ACAGGAAACA ACAGAAGCTT GTGAAAAGAA ATTAAGCCAA 
CTTGCTGAAG ACCTTAGAGA GAAGCACGAA GAAGAACTTC AGGAGAAAGA TCAGGTCATA 
GCAGAATCAA GAAAACAGTT TAATATCAAG AATAAAGAAA AAGAAGATGC AGACAAAGAG 
ATATCAGACC TCAGGGAACT GAAGACCCAA ATGGATGCCA CGTTTAATGA TTTGCAAGAA 
CAGCTGTGTC AGTCAGCAGC TCAAGTTTTA CATTTGACAC AGAGTGAAAA TGAATTCAAG 
ACAAATTGTA CTCGGCTAAC AACGGAGAAG CAAATTCTTC AACAACAGCT TGATGAACTG 
AAAATATTAG CAGAGGAAGA TAAAAGCAAG AATGATGAAA TAGTTAACAA GGTGAGACTT 
GCTGAAGAAA AGTATCAAGA CTTGGAGACC CTGCACAATA AAGTGAGAGC AGACTTTGCT 
AAGGAACTGC AGGATAAACT TGTTGAAAAG GAAAATGAGT TTAAACACCT TTTGGTTAAA 
CAAGAAAACC AGTTGATCTT GTGCTGCAAA AATGCTGAAG ATTTATTTGA AACACTTGCA 
AATGGACTCA CTGAAAACCA CAAATATAAG TTGAATGGAT TGATTGTTAA AATAGCAAAT 
CTTGAAAGCT GCATGCATAA ACTAAAAGAG GTCACTTTAA AACAGCGTAG TAGAATATAT 
GAACAAGAAA AACAACTTGA GCAGTTTTCA AACGAACTTC TTTCAGTAAA CCAATCTCTT 
AATCAAGCAA CTCAGGAGAA AGAATTTGCT ACTTTGTCGC TAAAGAATGA GATTGAAGCT 
CTTAATTTTC AAAATGAGAT GTTAAAAAAA GAAGGAGGAA GTCAACAGCA GGTTGCAGAT 
GAGAAAGAAT CTTGCATTAC TCAGCTGAAA AAAGAACTTT CAGAGAATAT AAATATTGTG 
ACTTCCTTGA CAGAAGTGGT AAAACAGAAA GAATCTGAGG TATCTTCTCT TTCTGCCTTA 
ATTAATGAAC TTAAACTGAA ACATGAGAAT TGTGTAGATT TAACAGAAAA AGAAAATGCA 
ATTGCTTTTC TCACTGCACA GCAGAAACAG AGTCAGCAAG AGCTACAGAA TCAAATTCAG 
GATCTAATGG CAAAGATTGA GGTTCTGAAT AAAGAAAAAT CTGCACAAAT AGATGAGGCT 
GCTAAACTTA AAAATAAGTT GGATGAATGG AAGAAAAAGG CAGAACTTAA ATTTGTGCAG 
AACCACAACA CTATTAAAGA GCTAAAAGAG AAAGTTGAAT CAGTCAATAA GCAAATTGTT 
GAAAAAGATG AAAAACTTAG GACAGTGACA GAAGAATTTG AATGTCATCG TAGCAATATC 
CAAGAAACAC AACTCATGAG TGAAAGCAAA GAATTTGAAT TAACTACCCA GTTGGAAGGC 
CAGAAATCTA AAATTTCGGA ATTGGAGGAC CAGTGTTCGA AGCTGATGTC TGAAAAGAAA 
AGTTTGATGG ATCAAATCGA AATTCAAACT ATTCAGCAGA GTGAAGATAA GAAAGCACTG 
GTTGAGCAAC TCCAGCATAT TCAGTATGCT TCTGACAATA ACTCCAAGGA AGTGCAAAAT 
AAAGTTATAA CACTTGAAAA AGAAATTGAT GCTTTAAAGG AGAAACTTGA TGCAGAGCAA 
ACAAAATGGC AGCTGGAGAA AGCAGAGTGC TTTCAGCTGA AAGATAAGGA ATTAAAAATA 
TTGGAAGAGA AACTAACTGC AGAAAGCACA AATAAACTTT CAGAGCTCAA GAAAAAAGCT 
GAACAGAAAA TCGCTTCTCT TAAGAAACAA CTTATGTCTC AGATAGATGA GAAGGAGCAG 
TCCAGGAAAG CGCTGGAAAG CCAGCTAAAT GTCTTAAAAG ATAATATGCA GAAAGAGAGC 
CAGGAAGTGC AGGAAGGATT GCAACAAAAA GTTGAAAATC AAGACAAAAA ATATGCATTG 
CTACTGGAGC AGAAGAAAAA TACAGAAGCA GATTTAGCTA ATATTAGAAG TGAACTAAAA 
TTAAAGGAAC TAAGAGTTAA AGAAATGGAA GATATTCTTA AAGATGTCCA GCAAAAACAT 
TCTGAAAGAG AGCTGACCGA CAGTGAGAAA GAACAAGCAC TTTTAAAAGA ACTAGAGTCA 
CAGATGCTGA CAAATAATCA GCTGGTTTCT ACTCATGAAA AAGCTTTAAG AGCCCTGCAA 
GATGACCTCA ATGTCAAGGA TGCAGAGATA AAGACCTTTA AAGAGGAAAT AGATGAGAAA 
TCCAAATCCT TAAATGAACT CCAAGTGCTT TTTGAGGAGA CTCAGTCTCA AGAGAGCGCA 
ATGAGAATAA AACTTGAGGA AATGGAGAGT GAGAAGCTAA AATTCAAGAA TGAAATAACT 
AAGCTACAGA AAGATATGCG CTCTTTACGT AAGGAACACA GCCAAGAACT AGATTTGCTT 
AAAAAGGAGA TAACCGAGGA AGCCGAACAA AGAATCAAAT ACGAACAGGA AGACTCGGAA 
CTTAAGCATA ATTCTAAATT AAAGCAACTC ATGAGAGAAT TCAATACCCA GATGGCTCAA 
AAGGAGCGTG AGGTTGAAAC AGCTGTTCAG GAGACTATTG CTAAAGCTCA GGAGGTTGAG 
GCTGAATTAA TGCGATCACA ACAGATAGAA GCAGTCCATC TTCATAAAAA GATTGCTGAG 
AAAGAAGATG ACCTAAAGAG AACTGTAAAA AGATATGAAG AACTTTTAGA GGCCCGAGAA 
GAGGAAATGA CAGCAAAAGT GACAGAGTTA CAAGAGCAAC TTGAAAGATT ACAGGAACAA 
TTAACTCAGA AGGAAATGCA AAATGAAGAA GAAGAAAATG TGGAGGAGGC AACATCTCCT 
GAGGTTTTTC AGGCGCAGCT GGCACAGAAA ACATCGCAGG TCAATGAATA CAAACTGAAG 
GAGCAGGAAC TTAAGGAGAA GGTTCACATC CTAGAAGACC AGGTGAAAAA CTTTACTCGT 
GGTGTATTTT ATACTCCTAT TGGCACACCG TATAAAGAAG TTAATAATCA ACATGCAGGG 
GTTTCTGTTT TTGGCGAACC CACTGAATTT GAGTACTTAA GAAAGGTGAT GTTTGAGTAC 
ATGATGGGGC GTGAAACAAA GACAATGGCA AAGGTAATAA CAACTGTGCT GAGGTTCCCA 
ACAGATCAAG CTCAACAAAT TTTGCAACGA GAAGAAACCC GACCACTGCC TCGCAGTGGT 
ATGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

golga4 ( XM_018094818.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017950307.1 Xenopus tropicalis golgin A4 (golga4), transcript variant X4, mRNA.

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CloneID	OXa45018
Clone ID Related Accession (Same CDS sequence)	XM_018094818.2 , XM_018094818.1
Accession Version	XM_018094818.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	golgin subfamily A member 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018094818.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAAGA AACTGAAACA GAAGATCAGC GAAGAGCAGT CCCCTTCCAA AAGCCCCCTT 
GCTCCACAGC AGCAGGGTCG TCCAAGAAGC ACATCAACAG GCAACAGAAG TCGGACTTCG 
TCAGTAACAG AACAGCAGGA TGACAGCCTG GCAACAGCAG ATAGAGAGAA CGGATCATTG 
TCGGACAATA TTAATGGAAA TGATCCCACT TCTTCTCAGA GGAATGAACC TCAGTCTTTT 
GCACATAAGC TACAAATGCG GGTTCCTTCA ATGGAATCAC TGTTTCGAAG CCCCATTAAA 
GAATCACTTT TTCCTTCTTC AACTAAAGAA AATCTTTTGC GGAGTAGTTC ACGGGATTCC 
CTGAATAAAA TTGATATGGA GAATCCAAGT ACTGTATTTG ATCCCCCATC CGATATTGAA 
AGTGAAGCTG AGGATTCTGT GGGAAGTATA GAAGGACTTA ACAAAGAACA GCTGTTTAAT 
CGACTACATA GAATGGATAA AAGTTTAGGA AACTACAGAG GAAAATATTC TGAGCTTGTA 
ACAGCCTACA GAACAATGCA GAGAGACAAA GAGAAACTAC AGTCCATCTT GAGTCAGAGC 
CAGGACAAAG CACTAAGGAG AATTGGAGAG CTGCGAGAGG AGCTTCAAAT GGATCAACAG 
GCTAAAAAGC ATCTACAGGA AGAGTTTGAT GCATCTCTGG AAGAGAAAGA TCAACTTATC 
AGTGTTCTGC AAACACAGGT TAGTTTACTT AAAAACAGAC TTCAGAATGG ACAAAGCAGT 
ACTGAATTAC CAGAAAGCAC AGCCTCGCCA GAACTTGACT CTGAGAGTTC CTTGCAAGAG 
GTGGATACAG ATAACCGTTC CACCCCTGAT GATGGGAATG CTGCAAACAC TTTAAATGCT 
CTCCAGGTTA GAGTAAAACG TCAAGAGAAC CTTTTGCAAA GGTGTAAGAA ATCAATCAAG 
TCGCACAAGG AACAGTGTGC TCACCTCAGT AGTGAAAATG AAGCTCTTCA AAACCAACTA 
GATGAAAGAC TCCAGGAACT TGAGAAAATT AAAGAACTTC ACACTAGTGA GAAGAGCAAG 
CTGATTACCC AGCTTAGAGA TGCCAAGAAT TCAATTGAAC AGCTGGAACA AGATAAGGGT 
ATGATGATTG CAGAAACAAA ACGGCAGATG CATGAGACAT TAGAACTGAA GGAAGAAGAG 
GTTTCTCAGC TTCGGTCAAG GGTCAAGAAA TTAAGTTCAC ATTGTGAGGA ACTGCAGCAA 
CAGAAAGAAA AGTTAGAGAA AGCTGCATTT GAAGATGTAG AAAAAATATC TTCACAGAAA 
GCTGAGGATG CTTTTGAAAA GCTGCAGATG GAAGAGCAAA TTAAAGCTAT AGAAAAGGCC 
AGTGAGGAGG AGCGTGTCAA TCTTCAGAAG GAATTAAGTC GTGTGAAGCA AGAGGTGGTG 
GACATTATGA AGAAATCCTG TGATAGTCGT ATTAGTGAGA TTGAGAAAGT TCATGCAGAA 
GCCTTGCTGA ATAAGGAACA GGAGTATAAT GAACAGATTC GCCTGAAGGA ACAAGAATTC 
CAGGATCAAA TGAATGCTGT CAGGATTCTT GACCTTGAGA GTTCTGTTGC TGAAGAAAGT 
AAGTCCCAAG ATTTTGTAAG CCTGATTGAA ACAGAGAGAA ATAATCACAA TGCAGAAGTA 
GCTACCATCA AAGAAATGCA TAAGCAAGAT CTTGATGTCC TTGTACAGGA TCAAGAAAGG 
ATATGGTCAG AAAAGCTTGA GCTGCTTAAG AAAGAGAATC ATACTAAGAT TATTGAACTT 
AAAGAGAAGT ATCAGCGAGA TTATGATTCT CACTTGAAAG AGAAAGAGCA TAATTTCCAG 
GAGCATATTG AGCATATGAA TGAAAAAACA TTAGAAAAAC TAGATGTAAA ACAAACTGAA 
TTGGAAACCC TCTCAGCTGA ACTGTCTGAG GCTATAAAGG CACGGCAAGA GATTGAGCAG 
AAACTTACAG ACACAGAAAA TGAGATTAGC AAAATAAAAC TAGAGTATGA AGCCCAGATA 
AAAGAAGAAA GAGAAAAACA CAGTGAAGAA ATTGGTTCCA TTGAAAGAGA GAAGGAAATG 
CATACTCAGG GTGTTGAGAA AGACTTGAAG GAAAATATTA ACAACCTAAA ACTACTTCAA 
GATAGTAAAG AATGTGAGTT GGAAGAATTC AGAAAGGAAA ATCAAAAGCT AAAAGAAGCT 
GCTGAAAAGG CAGAAGCAGA CCATAAAGAA GCATTTGCTC TATTGGAAGC AGCCAGGCAT 
TCTCATGAGA TAAATACACA TGAGAAAGAA CAGGCTTATG AGCTAAAATT GAAGGAGCTT 
CAGGAGATGC TAAAAGGTAT GACGCAGGAG CGAAACGATC TTAAAGAGGT GACAGAAAAA 
GCAGAAACAC AGTTAAAGAA TATGCAGATC GAGCTGGATT CTAACAAGGC CCAAGTACAA 
AGCCTTACAA AGCAAATTCA TGAGGAAAGT ATGAATATGG CAGATAAACT TAACACTTTG 
GTACTAGAGT CTGAAACTCA GGTAAAAGGG TTGAAGGAAC AAATAGAAGA GATAAGCAGA 
ATAGCTTCAG AGCGTGAACA TGAACTTAAA AAAGTAAAAG AAGATCATAT TCAAATTATT 
TTAGACCTAA ACAATACTAT ATCTGAAAAA GAGAAGAATA TACTTTCTTT GCAAGAAGAT 
TACAAAGTAA AACACAAAAA TCTGGATGCT AAAATGGAAA AATTAAAGCA GAAGTTTAAA 
GAGCTGCAAG AATCATCTAA AAAGAAATAT TCAGAGTTGG AAAGCAAACT TAAGAAAGAG 
ATCGAGAAGA AGCAGAACGA GTTGATTAAC AAAGAAAGGG AGTTTAATGA AAAAATACAT 
GAGATGGCCC AAGCAAGTTC AACTGGAATT AATCATGCAA TGGCTCAGCT TGAACTGAAT 
CATAAAGAGC AGATTCAGAA TATGACAGAC AATCATCAGA GAGAATTACA GGAAACAACA 
GAAGCTTGTG AAAAGAAATT AAGCCAACTT GCTGAAGACC TTAGAGAGAA GCACGAAGAA 
GAACTTCAGG AGAAAGATCA GGTCATAGCA GAATCAAGAA AACAGTTTAA TATCAAGAAT 
AAAGAAAAAG AAGATGCAGA CAAAGAGATA TCAGACCTCA GGGAACTGAA GACCCAAATG 
GATGCCACGT TTAATGATTT GCAAGAACAG CTGTGTCAGT CAGCAGCTCA AGTTTTACAT 
TTGACACAGA GTGAAAATGA ATTCAAGACA AATTGTACTC GGCTAACAAC GGAGAAGCAA 
ATTCTTCAAC AACAGCTTGA TGAACTGAAA ATATTAGCAG AGGAAGATAA AAGCAAGAAT 
GATGAAATAG TTAACAAGGT GAGACTTGCT GAAGAAAAGT ATCAAGACTT GGAGACCCTG 
CACAATAAAG TGAGAGCAGA CTTTGCTAAG GAACTGCAGG ATAAACTTGT TGAAAAGGAA 
AATGAGTTTA AACACCTTTT GGTTAAACAA GAAAACCAGT TGATCTTGTG CTGCAAAAAT 
GCTGAAGATT TATTTGAAAC ACTTGCAAAT GGACTCACTG AAAACCACAA ATATAAGTTG 
AATGGATTGA TTGTTAAAAT AGCAAATCTT GAAAGCTGCA TGCATAAACT AAAAGAGGTC 
ACTTTAAAAC AGCGTAGTAG AATATATGAA CAAGAAAAAC AACTTGAGCA GTTTTCAAAC 
GAACTTCTTT CAGTAAACCA ATCTCTTAAT CAAGCAACTC AGGAGAAAGA ATTTGCTACT 
TTGTCGCTAA AGAATGAGAT TGAAGCTCTT AATTTTCAAA ATGAGATGTT AAAAAAAGAA 
GGAGGAAGTC AACAGCAGGT TGCAGATGAG AAAGAATCTT GCATTACTCA GCTGAAAAAA 
GAACTTTCAG AGAATATAAA TATTGTGACT TCCTTGACAG AAGTGGTAAA ACAGAAAGAA 
TCTGAGGTAT CTTCTCTTTC TGCCTTAATT AATGAACTTA AACTGAAACA TGAGAATTGT 
GTAGATTTAA CAGAAAAAGA AAATGCAATT GCTTTTCTCA CTGCACAGCA GAAACAGAGT 
CAGCAAGAGC TACAGAATCA AATTCAGGAT CTAATGGCAA AGATTGAGGT TCTGAATAAA 
GAAAAATCTG CACAAATAGA TGAGGCTGCT AAACTTAAAA ATAAGTTGGA TGAATGGAAG 
AAAAAGGCAG AACTTAAATT TGTGCAGAAC CACAACACTA TTAAAGAGCT AAAAGAGAAA 
GTTGAATCAG TCAATAAGCA AATTGTTGAA AAAGATGAAA AACTTAGGAC AGTGACAGAA 
GAATTTGAAT GTCATCGTAG CAATATCCAA GAAACACAAC TCATGAGTGA AAGCAAAGAA 
TTTGAATTAA CTACCCAGTT GGAAGGCCAG AAATCTAAAA TTTCGGAATT GGAGGACCAG 
TGTTCGAAGC TGATGTCTGA AAAGAAAAGT TTGATGGATC AAATCGAAAT TCAAACTATT 
CAGCAGAGTG AAGATAAGAA AGCACTGGTT GAGCAACTCC AGCATATTCA GTATGCTTCT 
GACAATAACT CCAAGGAAGT GCAAAATAAA GTTATAACAC TTGAAAAAGA AATTGATGCT 
TTAAAGGAGA AACTTGATGC AGAGCAAACA AAATGGCAGC TGGAGAAAGC AGAGTGCTTT 
CAGCTGAAAG ATAAGGAATT AAAAATATTG GAAGAGAAAC TAACTGCAGA AAGCACAAAT 
AAACTTTCAG AGCTCAAGAA AAAAGCTGAA CAGAAAATCG CTTCTCTTAA GAAACAACTT 
ATGTCTCAGA TAGATGAGAA GGAGCAGTCC AGGAAAGCGC TGGAAAGCCA GCTAAATGTC 
TTAAAAGATA ATATGCAGAA AGAGAGCCAG GAAGTGCAGG AAGGATTGCA ACAAAAAGTT 
GAAAATCAAG ACAAAAAATA TGCATTGCTA CTGGAGCAGA AGAAAAATAC AGAAGCAGAT 
TTAGCTAATA TTAGAAGTGA ACTAAAATTA AAGGAACTAA GAGTTAAAGA AATGGAAGAT 
ATTCTTAAAG ATGTCCAGCA AAAACATTCT GAAAGAGAGC TGACCGACAG TGAGAAAGAA 
CAAGCACTTT TAAAAGAACT AGAGTCACAG ATGCTGACAA ATAATCAGCT GGTTTCTACT 
CATGAAAAAG CTTTAAGAGC CCTGCAAGAT GACCTCAATG TCAAGGATGC AGAGATAAAG 
ACCTTTAAAG AGGAAATAGA TGAGAAATCC AAATCCTTAA ATGAACTCCA AGTGCTTTTT 
GAGGAGACTC AGTCTCAAGA GAGCGCAATG AGAATAAAAC TTGAGGAAAT GGAGAGTGAG 
AAGCTAAAAT TCAAGAATGA AATAACTAAG CTACAGAAAG ATATGCGCTC TTTACGTAAG 
GAACACAGCC AAGAACTAGA TTTGCTTAAA AAGGAGATAA CCGAGGAAGC CGAACAAAGA 
ATCAAATACG AACAGGAAGA CTCGGAACTT AAGCATAATT CTAAATTAAA GCAACTCATG 
AGAGAATTCA ATACCCAGAT GGCTCAAAAG GAGCGTGAGG TTGAAACAGC TGTTCAGGAG 
ACTATTGCTA AAGCTCAGGA GGTTGAGGCT GAATTAATGC GATCACAACA GATAGAAGCA 
GTCCATCTTC ATAAAAAGAT TGCTGAGAAA GAAGATGACC TAAAGAGAAC TGTAAAAAGA 
TATGAAGAAC TTTTAGAGGC CCGAGAAGAG GAAATGACAG CAAAAGTGAC AGAGTTACAA 
GAGCAACTTG AAAGATTACA GGAACAATTA ACTCAGAAGG AAATGCAAAA TGAAGAAGAA 
GAAAATGTGG AGGAGGCAAC ATCTCCTGAG GTTTTTCAGG CGCAGCTGGC ACAGAAAACA 
TCGCAGGTCA ATGAATACAA ACTGAAGGAG CAGGAACTTA AGGAGAAGGT TCACATCCTA 
GAAGACCAGG TGAAAAACTT TACTCGTGGT GTATTTTATA CTCCTATTGG CACACCGTAT 
AAAGAAGTTA ATAATCAACA TGCAGGGGTT TCTGTTTTTG GCGAACCCAC TGAATTTGAG 
TACTTAAGAA AGGTGATGTT TGAGTACATG ATGGGGCGTG AAACAAAGAC AATGGCAAAG 
GTAATAACAA CTGTGCTGAG GTTCCCAACA GATCAAGCTC AACAAATTTT GCAACGAGAA 
GAAACCCGAC CACTGTCTTG GCTTCTATCT TCCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017950307.1
CDS	219..6437
Translation	MFKKLKQKISEEQSPSKSPLAPQQQGRPRSTSTGNRSRTSSVTEQQDDSLATADRENGSLSDNINGNDPTSSQRNEPQSFAHKLQMRVPSMESLFRSPIKESLFPSSTKENLLRSSSRDSLNKIDMENPSTVFDPPSDIESEAEDSVGSIEGLNKEQLFNRLHRMDKSLGNYRGKYSELVTAYRTMQRDKEKLQSILSQSQDKALRRIGELREELQMDQQAKKHLQEEFDASLEEKDQLISVLQTQVSLLKNRLQNGQSSTELPESTASPELDSESSLQEVDTDNRSTPDDGNAANTLNALQVRVKRQENLLQRCKKSIKSHKEQCAHLSSENEALQNQLDERLQELEKIKELHTSEKSKLITQLRDAKNSIEQLEQDKGMMIAETKRQMHETLELKEEEVSQLRSRVKKLSSHCEELQQQKEKLEKAAFEDVEKISSQKAEDAFEKLQMEEQIKAIEKASEEERVNLQKELSRVKQEVVDIMKKSCDSRISEIEKVHAEALLNKEQEYNEQIRLKEQEFQDQMNAVRILDLESSVAEESKSQDFVSLIETERNNHNAEVATIKEMHKQDLDVLVQDQERIWSEKLELLKKENHTKIIELKEKYQRDYDSHLKEKEHNFQEHIEHMNEKTLEKLDVKQTELETLSAELSEAIKARQEIEQKLTDTENEISKIKLEYEAQIKEEREKHSEEIGSIEREKEMHTQGVEKDLKENINNLKLLQDSKECELEEFRKENQKLKEAAEKAEADHKEAFALLEAARHSHEINTHEKEQAYELKLKELQEMLKGMTQERNDLKEVTEKAETQLKNMQIELDSNKAQVQSLTKQIHEESMNMADKLNTLVLESETQVKGLKEQIEEISRIASEREHELKKVKEDHIQIILDLNNTISEKEKNILSLQEDYKVKHKNLDAKMEKLKQKFKELQESSKKKYSELESKLKKEIEKKQNELINKEREFNEKIHEMAQASSTGINHAMAQLELNHKEQIQNMTDNHQRELQETTEACEKKLSQLAEDLREKHEEELQEKDQVIAESRKQFNIKNKEKEDADKEISDLRELKTQMDATFNDLQEQLCQSAAQVLHLTQSENEFKTNCTRLTTEKQILQQQLDELKILAEEDKSKNDEIVNKVRLAEEKYQDLETLHNKVRADFAKELQDKLVEKENEFKHLLVKQENQLILCCKNAEDLFETLANGLTENHKYKLNGLIVKIANLESCMHKLKEVTLKQRSRIYEQEKQLEQFSNELLSVNQSLNQATQEKEFATLSLKNEIEALNFQNEMLKKEGGSQQQVADEKESCITQLKKELSENINIVTSLTEVVKQKESEVSSLSALINELKLKHENCVDLTEKENAIAFLTAQQKQSQQELQNQIQDLMAKIEVLNKEKSAQIDEAAKLKNKLDEWKKKAELKFVQNHNTIKELKEKVESVNKQIVEKDEKLRTVTEEFECHRSNIQETQLMSESKEFELTTQLEGQKSKISELEDQCSKLMSEKKSLMDQIEIQTIQQSEDKKALVEQLQHIQYASDNNSKEVQNKVITLEKEIDALKEKLDAEQTKWQLEKAECFQLKDKELKILEEKLTAESTNKLSELKKKAEQKIASLKKQLMSQIDEKEQSRKALESQLNVLKDNMQKESQEVQEGLQQKVENQDKKYALLLEQKKNTEADLANIRSELKLKELRVKEMEDILKDVQQKHSERELTDSEKEQALLKELESQMLTNNQLVSTHEKALRALQDDLNVKDAEIKTFKEEIDEKSKSLNELQVLFEETQSQESAMRIKLEEMESEKLKFKNEITKLQKDMRSLRKEHSQELDLLKKEITEEAEQRIKYEQEDSELKHNSKLKQLMREFNTQMAQKEREVETAVQETIAKAQEVEAELMRSQQIEAVHLHKKIAEKEDDLKRTVKRYEELLEAREEEMTAKVTELQEQLERLQEQLTQKEMQNEEEENVEEATSPEVFQAQLAQKTSQVNEYKLKEQELKEKVHILEDQVKNFTRGVFYTPIGTPYKEVNNQHAGVSVFGEPTEFEYLRKVMFEYMMGRETKTMAKVITTVLRFPTDQAQQILQREETRPLSWLLSSS

Target ORF information:

RefSeq Version	XM_018094818.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis golgin A4 (golga4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094818.2

ORF Insert Sequence:

ATGTTTAAGA AACTGAAACA GAAGATCAGC GAAGAGCAGT CCCCTTCCAA AAGCCCCCTT 
GCTCCACAGC AGCAGGGTCG TCCAAGAAGC ACATCAACAG GCAACAGAAG TCGGACTTCG 
TCAGTAACAG AACAGCAGGA TGACAGCCTG GCAACAGCAG ATAGAGAGAA CGGATCATTG 
TCGGACAATA TTAATGGAAA TGATCCCACT TCTTCTCAGA GGAATGAACC TCAGTCTTTT 
GCACATAAGC TACAAATGCG GGTTCCTTCA ATGGAATCAC TGTTTCGAAG CCCCATTAAA 
GAATCACTTT TTCCTTCTTC AACTAAAGAA AATCTTTTGC GGAGTAGTTC ACGGGATTCC 
CTGAATAAAA TTGATATGGA GAATCCAAGT ACTGTATTTG ATCCCCCATC CGATATTGAA 
AGTGAAGCTG AGGATTCTGT GGGAAGTATA GAAGGACTTA ACAAAGAACA GCTGTTTAAT 
CGACTACATA GAATGGATAA AAGTTTAGGA AACTACAGAG GAAAATATTC TGAGCTTGTA 
ACAGCCTACA GAACAATGCA GAGAGACAAA GAGAAACTAC AGTCCATCTT GAGTCAGAGC 
CAGGACAAAG CACTAAGGAG AATTGGAGAG CTGCGAGAGG AGCTTCAAAT GGATCAACAG 
GCTAAAAAGC ATCTACAGGA AGAGTTTGAT GCATCTCTGG AAGAGAAAGA TCAACTTATC 
AGTGTTCTGC AAACACAGGT TAGTTTACTT AAAAACAGAC TTCAGAATGG ACAAAGCAGT 
ACTGAATTAC CAGAAAGCAC AGCCTCGCCA GAACTTGACT CTGAGAGTTC CTTGCAAGAG 
GTGGATACAG ATAACCGTTC CACCCCTGAT GATGGGAATG CTGCAAACAC TTTAAATGCT 
CTCCAGGTTA GAGTAAAACG TCAAGAGAAC CTTTTGCAAA GGTGTAAGAA ATCAATCAAG 
TCGCACAAGG AACAGTGTGC TCACCTCAGT AGTGAAAATG AAGCTCTTCA AAACCAACTA 
GATGAAAGAC TCCAGGAACT TGAGAAAATT AAAGAACTTC ACACTAGTGA GAAGAGCAAG 
CTGATTACCC AGCTTAGAGA TGCCAAGAAT TCAATTGAAC AGCTGGAACA AGATAAGGGT 
ATGATGATTG CAGAAACAAA ACGGCAGATG CATGAGACAT TAGAACTGAA GGAAGAAGAG 
GTTTCTCAGC TTCGGTCAAG GGTCAAGAAA TTAAGTTCAC ATTGTGAGGA ACTGCAGCAA 
CAGAAAGAAA AGTTAGAGAA AGCTGCATTT GAAGATGTAG AAAAAATATC TTCACAGAAA 
GCTGAGGATG CTTTTGAAAA GCTGCAGATG GAAGAGCAAA TTAAAGCTAT AGAAAAGGCC 
AGTGAGGAGG AGCGTGTCAA TCTTCAGAAG GAATTAAGTC GTGTGAAGCA AGAGGTGGTG 
GACATTATGA AGAAATCCTG TGATAGTCGT ATTAGTGAGA TTGAGAAAGT TCATGCAGAA 
GCCTTGCTGA ATAAGGAACA GGAGTATAAT GAACAGATTC GCCTGAAGGA ACAAGAATTC 
CAGGATCAAA TGAATGCTGT CAGGATTCTT GACCTTGAGA GTTCTGTTGC TGAAGAAAGT 
AAGTCCCAAG ATTTTGTAAG CCTGATTGAA ACAGAGAGAA ATAATCACAA TGCAGAAGTA 
GCTACCATCA AAGAAATGCA TAAGCAAGAT CTTGATGTCC TTGTACAGGA TCAAGAAAGG 
ATATGGTCAG AAAAGCTTGA GCTGCTTAAG AAAGAGAATC ATACTAAGAT TATTGAACTT 
AAAGAGAAGT ATCAGCGAGA TTATGATTCT CACTTGAAAG AGAAAGAGCA TAATTTCCAG 
GAGCATATTG AGCATATGAA TGAAAAAACA TTAGAAAAAC TAGATGTAAA ACAAACTGAA 
TTGGAAACCC TCTCAGCTGA ACTGTCTGAG GCTATAAAGG CACGGCAAGA GATTGAGCAG 
AAACTTACAG ACACAGAAAA TGAGATTAGC AAAATAAAAC TAGAGTATGA AGCCCAGATA 
AAAGAAGAAA GAGAAAAACA CAGTGAAGAA ATTGGTTCCA TTGAAAGAGA GAAGGAAATG 
CATACTCAGG GTGTTGAGAA AGACTTGAAG GAAAATATTA ACAACCTAAA ACTACTTCAA 
GATAGTAAAG AATGTGAGTT GGAAGAATTC AGAAAGGAAA ATCAAAAGCT AAAAGAAGCT 
GCTGAAAAGG CAGAAGCAGA CCATAAAGAA GCATTTGCTC TATTGGAAGC AGCCAGGCAT 
TCTCATGAGA TAAATACACA TGAGAAAGAA CAGGCTTATG AGCTAAAATT GAAGGAGCTT 
CAGGAGATGC TAAAAGGTAT GACGCAGGAG CGAAACGATC TTAAAGAGGT GACAGAAAAA 
GCAGAAACAC AGTTAAAGAA TATGCAGATC GAGCTGGATT CTAACAAGGC CCAAGTACAA 
AGCCTTACAA AGCAAATTCA TGAGGAAAGT ATGAATATGG CAGATAAACT TAACACTTTG 
GTACTAGAGT CTGAAACTCA GGTAAAAGGG TTGAAGGAAC AAATAGAAGA GATAAGCAGA 
ATAGCTTCAG AGCGTGAACA TGAACTTAAA AAAGTAAAAG AAGATCATAT TCAAATTATT 
TTAGACCTAA ACAATACTAT ATCTGAAAAA GAGAAGAATA TACTTTCTTT GCAAGAAGAT 
TACAAAGTAA AACACAAAAA TCTGGATGCT AAAATGGAAA AATTAAAGCA GAAGTTTAAA 
GAGCTGCAAG AATCATCTAA AAAGAAATAT TCAGAGTTGG AAAGCAAACT TAAGAAAGAG 
ATCGAGAAGA AGCAGAACGA GTTGATTAAC AAAGAAAGGG AGTTTAATGA AAAAATACAT 
GAGATGGCCC AAGCAAGTTC AACTGGAATT AATCATGCAA TGGCTCAGCT TGAACTGAAT 
CATAAAGAGC AGATTCAGAA TATGACAGAC AATCATCAGA GAGAATTACA GGAAACAACA 
GAAGCTTGTG AAAAGAAATT AAGCCAACTT GCTGAAGACC TTAGAGAGAA GCACGAAGAA 
GAACTTCAGG AGAAAGATCA GGTCATAGCA GAATCAAGAA AACAGTTTAA TATCAAGAAT 
AAAGAAAAAG AAGATGCAGA CAAAGAGATA TCAGACCTCA GGGAACTGAA GACCCAAATG 
GATGCCACGT TTAATGATTT GCAAGAACAG CTGTGTCAGT CAGCAGCTCA AGTTTTACAT 
TTGACACAGA GTGAAAATGA ATTCAAGACA AATTGTACTC GGCTAACAAC GGAGAAGCAA 
ATTCTTCAAC AACAGCTTGA TGAACTGAAA ATATTAGCAG AGGAAGATAA AAGCAAGAAT 
GATGAAATAG TTAACAAGGT GAGACTTGCT GAAGAAAAGT ATCAAGACTT GGAGACCCTG 
CACAATAAAG TGAGAGCAGA CTTTGCTAAG GAACTGCAGG ATAAACTTGT TGAAAAGGAA 
AATGAGTTTA AACACCTTTT GGTTAAACAA GAAAACCAGT TGATCTTGTG CTGCAAAAAT 
GCTGAAGATT TATTTGAAAC ACTTGCAAAT GGACTCACTG AAAACCACAA ATATAAGTTG 
AATGGATTGA TTGTTAAAAT AGCAAATCTT GAAAGCTGCA TGCATAAACT AAAAGAGGTC 
ACTTTAAAAC AGCGTAGTAG AATATATGAA CAAGAAAAAC AACTTGAGCA GTTTTCAAAC 
GAACTTCTTT CAGTAAACCA ATCTCTTAAT CAAGCAACTC AGGAGAAAGA ATTTGCTACT 
TTGTCGCTAA AGAATGAGAT TGAAGCTCTT AATTTTCAAA ATGAGATGTT AAAAAAAGAA 
GGAGGAAGTC AACAGCAGGT TGCAGATGAG AAAGAATCTT GCATTACTCA GCTGAAAAAA 
GAACTTTCAG AGAATATAAA TATTGTGACT TCCTTGACAG AAGTGGTAAA ACAGAAAGAA 
TCTGAGGTAT CTTCTCTTTC TGCCTTAATT AATGAACTTA AACTGAAACA TGAGAATTGT 
GTAGATTTAA CAGAAAAAGA AAATGCAATT GCTTTTCTCA CTGCACAGCA GAAACAGAGT 
CAGCAAGAGC TACAGAATCA AATTCAGGAT CTAATGGCAA AGATTGAGGT TCTGAATAAA 
GAAAAATCTG CACAAATAGA TGAGGCTGCT AAACTTAAAA ATAAGTTGGA TGAATGGAAG 
AAAAAGGCAG AACTTAAATT TGTGCAGAAC CACAACACTA TTAAAGAGCT AAAAGAGAAA 
GTTGAATCAG TCAATAAGCA AATTGTTGAA AAAGATGAAA AACTTAGGAC AGTGACAGAA 
GAATTTGAAT GTCATCGTAG CAATATCCAA GAAACACAAC TCATGAGTGA AAGCAAAGAA 
TTTGAATTAA CTACCCAGTT GGAAGGCCAG AAATCTAAAA TTTCGGAATT GGAGGACCAG 
TGTTCGAAGC TGATGTCTGA AAAGAAAAGT TTGATGGATC AAATCGAAAT TCAAACTATT 
CAGCAGAGTG AAGATAAGAA AGCACTGGTT GAGCAACTCC AGCATATTCA GTATGCTTCT 
GACAATAACT CCAAGGAAGT GCAAAATAAA GTTATAACAC TTGAAAAAGA AATTGATGCT 
TTAAAGGAGA AACTTGATGC AGAGCAAACA AAATGGCAGC TGGAGAAAGC AGAGTGCTTT 
CAGCTGAAAG ATAAGGAATT AAAAATATTG GAAGAGAAAC TAACTGCAGA AAGCACAAAT 
AAACTTTCAG AGCTCAAGAA AAAAGCTGAA CAGAAAATCG CTTCTCTTAA GAAACAACTT 
ATGTCTCAGA TAGATGAGAA GGAGCAGTCC AGGAAAGCGC TGGAAAGCCA GCTAAATGTC 
TTAAAAGATA ATATGCAGAA AGAGAGCCAG GAAGTGCAGG AAGGATTGCA ACAAAAAGTT 
GAAAATCAAG ACAAAAAATA TGCATTGCTA CTGGAGCAGA AGAAAAATAC AGAAGCAGAT 
TTAGCTAATA TTAGAAGTGA ACTAAAATTA AAGGAACTAA GAGTTAAAGA AATGGAAGAT 
ATTCTTAAAG ATGTCCAGCA AAAACATTCT GAAAGAGAGC TGACCGACAG TGAGAAAGAA 
CAAGCACTTT TAAAAGAACT AGAGTCACAG ATGCTGACAA ATAATCAGCT GGTTTCTACT 
CATGAAAAAG CTTTAAGAGC CCTGCAAGAT GACCTCAATG TCAAGGATGC AGAGATAAAG 
ACCTTTAAAG AGGAAATAGA TGAGAAATCC AAATCCTTAA ATGAACTCCA AGTGCTTTTT 
GAGGAGACTC AGTCTCAAGA GAGCGCAATG AGAATAAAAC TTGAGGAAAT GGAGAGTGAG 
AAGCTAAAAT TCAAGAATGA AATAACTAAG CTACAGAAAG ATATGCGCTC TTTACGTAAG 
GAACACAGCC AAGAACTAGA TTTGCTTAAA AAGGAGATAA CCGAGGAAGC CGAACAAAGA 
ATCAAATACG AACAGGAAGA CTCGGAACTT AAGCATAATT CTAAATTAAA GCAACTCATG 
AGAGAATTCA ATACCCAGAT GGCTCAAAAG GAGCGTGAGG TTGAAACAGC TGTTCAGGAG 
ACTATTGCTA AAGCTCAGGA GGTTGAGGCT GAATTAATGC GATCACAACA GATAGAAGCA 
GTCCATCTTC ATAAAAAGAT TGCTGAGAAA GAAGATGACC TAAAGAGAAC TGTAAAAAGA 
TATGAAGAAC TTTTAGAGGC CCGAGAAGAG GAAATGACAG CAAAAGTGAC AGAGTTACAA 
GAGCAACTTG AAAGATTACA GGAACAATTA ACTCAGAAGG AAATGCAAAA TGAAGAAGAA 
GAAAATGTGG AGGAGGCAAC ATCTCCTGAG GTTTTTCAGG CGCAGCTGGC ACAGAAAACA 
TCGCAGGTCA ATGAATACAA ACTGAAGGAG CAGGAACTTA AGGAGAAGGT TCACATCCTA 
GAAGACCAGG TGAAAAACTT TACTCGTGGT GTATTTTATA CTCCTATTGG CACACCGTAT 
AAAGAAGTTA ATAATCAACA TGCAGGGGTT TCTGTTTTTG GCGAACCCAC TGAATTTGAG 
TACTTAAGAA AGGTGATGTT TGAGTACATG ATGGGGCGTG AAACAAAGAC AATGGCAAAG 
GTAATAACAA CTGTGCTGAG GTTCCCAACA GATCAAGCTC AACAAATTTT GCAACGAGAA 
GAAACCCGAC CACTGTCTTG GCTTCTATCT TCCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

golga4 ( XM_018094818.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017950307.1 Xenopus tropicalis golgin A4 (golga4), transcript variant X4, mRNA.

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CloneID	OXa45018
Clone ID Related Accession (Same CDS sequence)	XM_018094818.2 , XM_018094818.1
Accession Version	XM_018094818.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	golgin subfamily A member 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAAGA AACTGAAACA GAAGATCAGC GAAGAGCAGT CCCCTTCCAA AAGCCCCCTT 
GCTCCACAGC AGCAGGGTCG TCCAAGAAGC ACATCAACAG GCAACAGAAG TCGGACTTCG 
TCAGTAACAG AACAGCAGGA TGACAGCCTG GCAACAGCAG ATAGAGAGAA CGGATCATTG 
TCGGACAATA TTAATGGAAA TGATCCCACT TCTTCTCAGA GGAATGAACC TCAGTCTTTT 
GCACATAAGC TACAAATGCG GGTTCCTTCA ATGGAATCAC TGTTTCGAAG CCCCATTAAA 
GAATCACTTT TTCCTTCTTC AACTAAAGAA AATCTTTTGC GGAGTAGTTC ACGGGATTCC 
CTGAATAAAA TTGATATGGA GAATCCAAGT ACTGTATTTG ATCCCCCATC CGATATTGAA 
AGTGAAGCTG AGGATTCTGT GGGAAGTATA GAAGGACTTA ACAAAGAACA GCTGTTTAAT 
CGACTACATA GAATGGATAA AAGTTTAGGA AACTACAGAG GAAAATATTC TGAGCTTGTA 
ACAGCCTACA GAACAATGCA GAGAGACAAA GAGAAACTAC AGTCCATCTT GAGTCAGAGC 
CAGGACAAAG CACTAAGGAG AATTGGAGAG CTGCGAGAGG AGCTTCAAAT GGATCAACAG 
GCTAAAAAGC ATCTACAGGA AGAGTTTGAT GCATCTCTGG AAGAGAAAGA TCAACTTATC 
AGTGTTCTGC AAACACAGGT TAGTTTACTT AAAAACAGAC TTCAGAATGG ACAAAGCAGT 
ACTGAATTAC CAGAAAGCAC AGCCTCGCCA GAACTTGACT CTGAGAGTTC CTTGCAAGAG 
GTGGATACAG ATAACCGTTC CACCCCTGAT GATGGGAATG CTGCAAACAC TTTAAATGCT 
CTCCAGGTTA GAGTAAAACG TCAAGAGAAC CTTTTGCAAA GGTGTAAGAA ATCAATCAAG 
TCGCACAAGG AACAGTGTGC TCACCTCAGT AGTGAAAATG AAGCTCTTCA AAACCAACTA 
GATGAAAGAC TCCAGGAACT TGAGAAAATT AAAGAACTTC ACACTAGTGA GAAGAGCAAG 
CTGATTACCC AGCTTAGAGA TGCCAAGAAT TCAATTGAAC AGCTGGAACA AGATAAGGGT 
ATGATGATTG CAGAAACAAA ACGGCAGATG CATGAGACAT TAGAACTGAA GGAAGAAGAG 
GTTTCTCAGC TTCGGTCAAG GGTCAAGAAA TTAAGTTCAC ATTGTGAGGA ACTGCAGCAA 
CAGAAAGAAA AGTTAGAGAA AGCTGCATTT GAAGATGTAG AAAAAATATC TTCACAGAAA 
GCTGAGGATG CTTTTGAAAA GCTGCAGATG GAAGAGCAAA TTAAAGCTAT AGAAAAGGCC 
AGTGAGGAGG AGCGTGTCAA TCTTCAGAAG GAATTAAGTC GTGTGAAGCA AGAGGTGGTG 
GACATTATGA AGAAATCCTG TGATAGTCGT ATTAGTGAGA TTGAGAAAGT TCATGCAGAA 
GCCTTGCTGA ATAAGGAACA GGAGTATAAT GAACAGATTC GCCTGAAGGA ACAAGAATTC 
CAGGATCAAA TGAATGCTGT CAGGATTCTT GACCTTGAGA GTTCTGTTGC TGAAGAAAGT 
AAGTCCCAAG ATTTTGTAAG CCTGATTGAA ACAGAGAGAA ATAATCACAA TGCAGAAGTA 
GCTACCATCA AAGAAATGCA TAAGCAAGAT CTTGATGTCC TTGTACAGGA TCAAGAAAGG 
ATATGGTCAG AAAAGCTTGA GCTGCTTAAG AAAGAGAATC ATACTAAGAT TATTGAACTT 
AAAGAGAAGT ATCAGCGAGA TTATGATTCT CACTTGAAAG AGAAAGAGCA TAATTTCCAG 
GAGCATATTG AGCATATGAA TGAAAAAACA TTAGAAAAAC TAGATGTAAA ACAAACTGAA 
TTGGAAACCC TCTCAGCTGA ACTGTCTGAG GCTATAAAGG CACGGCAAGA GATTGAGCAG 
AAACTTACAG ACACAGAAAA TGAGATTAGC AAAATAAAAC TAGAGTATGA AGCCCAGATA 
AAAGAAGAAA GAGAAAAACA CAGTGAAGAA ATTGGTTCCA TTGAAAGAGA GAAGGAAATG 
CATACTCAGG GTGTTGAGAA AGACTTGAAG GAAAATATTA ACAACCTAAA ACTACTTCAA 
GATAGTAAAG AATGTGAGTT GGAAGAATTC AGAAAGGAAA ATCAAAAGCT AAAAGAAGCT 
GCTGAAAAGG CAGAAGCAGA CCATAAAGAA GCATTTGCTC TATTGGAAGC AGCCAGGCAT 
TCTCATGAGA TAAATACACA TGAGAAAGAA CAGGCTTATG AGCTAAAATT GAAGGAGCTT 
CAGGAGATGC TAAAAGGTAT GACGCAGGAG CGAAACGATC TTAAAGAGGT GACAGAAAAA 
GCAGAAACAC AGTTAAAGAA TATGCAGATC GAGCTGGATT CTAACAAGGC CCAAGTACAA 
AGCCTTACAA AGCAAATTCA TGAGGAAAGT ATGAATATGG CAGATAAACT TAACACTTTG 
GTACTAGAGT CTGAAACTCA GGTAAAAGGG TTGAAGGAAC AAATAGAAGA GATAAGCAGA 
ATAGCTTCAG AGCGTGAACA TGAACTTAAA AAAGTAAAAG AAGATCATAT TCAAATTATT 
TTAGACCTAA ACAATACTAT ATCTGAAAAA GAGAAGAATA TACTTTCTTT GCAAGAAGAT 
TACAAAGTAA AACACAAAAA TCTGGATGCT AAAATGGAAA AATTAAAGCA GAAGTTTAAA 
GAGCTGCAAG AATCATCTAA AAAGAAATAT TCAGAGTTGG AAAGCAAACT TAAGAAAGAG 
ATCGAGAAGA AGCAGAACGA GTTGATTAAC AAAGAAAGGG AGTTTAATGA AAAAATACAT 
GAGATGGCCC AAGCAAGTTC AACTGGAATT AATCATGCAA TGGCTCAGCT TGAACTGAAT 
CATAAAGAGC AGATTCAGAA TATGACAGAC AATCATCAGA GAGAATTACA GGAAACAACA 
GAAGCTTGTG AAAAGAAATT AAGCCAACTT GCTGAAGACC TTAGAGAGAA GCACGAAGAA 
GAACTTCAGG AGAAAGATCA GGTCATAGCA GAATCAAGAA AACAGTTTAA TATCAAGAAT 
AAAGAAAAAG AAGATGCAGA CAAAGAGATA TCAGACCTCA GGGAACTGAA GACCCAAATG 
GATGCCACGT TTAATGATTT GCAAGAACAG CTGTGTCAGT CAGCAGCTCA AGTTTTACAT 
TTGACACAGA GTGAAAATGA ATTCAAGACA AATTGTACTC GGCTAACAAC GGAGAAGCAA 
ATTCTTCAAC AACAGCTTGA TGAACTGAAA ATATTAGCAG AGGAAGATAA AAGCAAGAAT 
GATGAAATAG TTAACAAGGT GAGACTTGCT GAAGAAAAGT ATCAAGACTT GGAGACCCTG 
CACAATAAAG TGAGAGCAGA CTTTGCTAAG GAACTGCAGG ATAAACTTGT TGAAAAGGAA 
AATGAGTTTA AACACCTTTT GGTTAAACAA GAAAACCAGT TGATCTTGTG CTGCAAAAAT 
GCTGAAGATT TATTTGAAAC ACTTGCAAAT GGACTCACTG AAAACCACAA ATATAAGTTG 
AATGGATTGA TTGTTAAAAT AGCAAATCTT GAAAGCTGCA TGCATAAACT AAAAGAGGTC 
ACTTTAAAAC AGCGTAGTAG AATATATGAA CAAGAAAAAC AACTTGAGCA GTTTTCAAAC 
GAACTTCTTT CAGTAAACCA ATCTCTTAAT CAAGCAACTC AGGAGAAAGA ATTTGCTACT 
TTGTCGCTAA AGAATGAGAT TGAAGCTCTT AATTTTCAAA ATGAGATGTT AAAAAAAGAA 
GGAGGAAGTC AACAGCAGGT TGCAGATGAG AAAGAATCTT GCATTACTCA GCTGAAAAAA 
GAACTTTCAG AGAATATAAA TATTGTGACT TCCTTGACAG AAGTGGTAAA ACAGAAAGAA 
TCTGAGGTAT CTTCTCTTTC TGCCTTAATT AATGAACTTA AACTGAAACA TGAGAATTGT 
GTAGATTTAA CAGAAAAAGA AAATGCAATT GCTTTTCTCA CTGCACAGCA GAAACAGAGT 
CAGCAAGAGC TACAGAATCA AATTCAGGAT CTAATGGCAA AGATTGAGGT TCTGAATAAA 
GAAAAATCTG CACAAATAGA TGAGGCTGCT AAACTTAAAA ATAAGTTGGA TGAATGGAAG 
AAAAAGGCAG AACTTAAATT TGTGCAGAAC CACAACACTA TTAAAGAGCT AAAAGAGAAA 
GTTGAATCAG TCAATAAGCA AATTGTTGAA AAAGATGAAA AACTTAGGAC AGTGACAGAA 
GAATTTGAAT GTCATCGTAG CAATATCCAA GAAACACAAC TCATGAGTGA AAGCAAAGAA 
TTTGAATTAA CTACCCAGTT GGAAGGCCAG AAATCTAAAA TTTCGGAATT GGAGGACCAG 
TGTTCGAAGC TGATGTCTGA AAAGAAAAGT TTGATGGATC AAATCGAAAT TCAAACTATT 
CAGCAGAGTG AAGATAAGAA AGCACTGGTT GAGCAACTCC AGCATATTCA GTATGCTTCT 
GACAATAACT CCAAGGAAGT GCAAAATAAA GTTATAACAC TTGAAAAAGA AATTGATGCT 
TTAAAGGAGA AACTTGATGC AGAGCAAACA AAATGGCAGC TGGAGAAAGC AGAGTGCTTT 
CAGCTGAAAG ATAAGGAATT AAAAATATTG GAAGAGAAAC TAACTGCAGA AAGCACAAAT 
AAACTTTCAG AGCTCAAGAA AAAAGCTGAA CAGAAAATCG CTTCTCTTAA GAAACAACTT 
ATGTCTCAGA TAGATGAGAA GGAGCAGTCC AGGAAAGCGC TGGAAAGCCA GCTAAATGTC 
TTAAAAGATA ATATGCAGAA AGAGAGCCAG GAAGTGCAGG AAGGATTGCA ACAAAAAGTT 
GAAAATCAAG ACAAAAAATA TGCATTGCTA CTGGAGCAGA AGAAAAATAC AGAAGCAGAT 
TTAGCTAATA TTAGAAGTGA ACTAAAATTA AAGGAACTAA GAGTTAAAGA AATGGAAGAT 
ATTCTTAAAG ATGTCCAGCA AAAACATTCT GAAAGAGAGC TGACCGACAG TGAGAAAGAA 
CAAGCACTTT TAAAAGAACT AGAGTCACAG ATGCTGACAA ATAATCAGCT GGTTTCTACT 
CATGAAAAAG CTTTAAGAGC CCTGCAAGAT GACCTCAATG TCAAGGATGC AGAGATAAAG 
ACCTTTAAAG AGGAAATAGA TGAGAAATCC AAATCCTTAA ATGAACTCCA AGTGCTTTTT 
GAGGAGACTC AGTCTCAAGA GAGCGCAATG AGAATAAAAC TTGAGGAAAT GGAGAGTGAG 
AAGCTAAAAT TCAAGAATGA AATAACTAAG CTACAGAAAG ATATGCGCTC TTTACGTAAG 
GAACACAGCC AAGAACTAGA TTTGCTTAAA AAGGAGATAA CCGAGGAAGC CGAACAAAGA 
ATCAAATACG AACAGGAAGA CTCGGAACTT AAGCATAATT CTAAATTAAA GCAACTCATG 
AGAGAATTCA ATACCCAGAT GGCTCAAAAG GAGCGTGAGG TTGAAACAGC TGTTCAGGAG 
ACTATTGCTA AAGCTCAGGA GGTTGAGGCT GAATTAATGC GATCACAACA GATAGAAGCA 
GTCCATCTTC ATAAAAAGAT TGCTGAGAAA GAAGATGACC TAAAGAGAAC TGTAAAAAGA 
TATGAAGAAC TTTTAGAGGC CCGAGAAGAG GAAATGACAG CAAAAGTGAC AGAGTTACAA 
GAGCAACTTG AAAGATTACA GGAACAATTA ACTCAGAAGG AAATGCAAAA TGAAGAAGAA 
GAAAATGTGG AGGAGGCAAC ATCTCCTGAG GTTTTTCAGG CGCAGCTGGC ACAGAAAACA 
TCGCAGGTCA ATGAATACAA ACTGAAGGAG CAGGAACTTA AGGAGAAGGT TCACATCCTA 
GAAGACCAGG TGAAAAACTT TACTCGTGGT GTATTTTATA CTCCTATTGG CACACCGTAT 
AAAGAAGTTA ATAATCAACA TGCAGGGGTT TCTGTTTTTG GCGAACCCAC TGAATTTGAG 
TACTTAAGAA AGGTGATGTT TGAGTACATG ATGGGGCGTG AAACAAAGAC AATGGCAAAG 
GTAATAACAA CTGTGCTGAG GTTCCCAACA GATCAAGCTC AACAAATTTT GCAACGAGAA 
GAAACCCGAC CACTGTCTTG GCTTCTATCT TCCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017950307.1
CDS	681..6899
Translation	MFKKLKQKISEEQSPSKSPLAPQQQGRPRSTSTGNRSRTSSVTEQQDDSLATADRENGSLSDNINGNDPTSSQRNEPQSFAHKLQMRVPSMESLFRSPIKESLFPSSTKENLLRSSSRDSLNKIDMENPSTVFDPPSDIESEAEDSVGSIEGLNKEQLFNRLHRMDKSLGNYRGKYSELVTAYRTMQRDKEKLQSILSQSQDKALRRIGELREELQMDQQAKKHLQEEFDASLEEKDQLISVLQTQVSLLKNRLQNGQSSTELPESTASPELDSESSLQEVDTDNRSTPDDGNAANTLNALQVRVKRQENLLQRCKKSIKSHKEQCAHLSSENEALQNQLDERLQELEKIKELHTSEKSKLITQLRDAKNSIEQLEQDKGMMIAETKRQMHETLELKEEEVSQLRSRVKKLSSHCEELQQQKEKLEKAAFEDVEKISSQKAEDAFEKLQMEEQIKAIEKASEEERVNLQKELSRVKQEVVDIMKKSCDSRISEIEKVHAEALLNKEQEYNEQIRLKEQEFQDQMNAVRILDLESSVAEESKSQDFVSLIETERNNHNAEVATIKEMHKQDLDVLVQDQERIWSEKLELLKKENHTKIIELKEKYQRDYDSHLKEKEHNFQEHIEHMNEKTLEKLDVKQTELETLSAELSEAIKARQEIEQKLTDTENEISKIKLEYEAQIKEEREKHSEEIGSIEREKEMHTQGVEKDLKENINNLKLLQDSKECELEEFRKENQKLKEAAEKAEADHKEAFALLEAARHSHEINTHEKEQAYELKLKELQEMLKGMTQERNDLKEVTEKAETQLKNMQIELDSNKAQVQSLTKQIHEESMNMADKLNTLVLESETQVKGLKEQIEEISRIASEREHELKKVKEDHIQIILDLNNTISEKEKNILSLQEDYKVKHKNLDAKMEKLKQKFKELQESSKKKYSELESKLKKEIEKKQNELINKEREFNEKIHEMAQASSTGINHAMAQLELNHKEQIQNMTDNHQRELQETTEACEKKLSQLAEDLREKHEEELQEKDQVIAESRKQFNIKNKEKEDADKEISDLRELKTQMDATFNDLQEQLCQSAAQVLHLTQSENEFKTNCTRLTTEKQILQQQLDELKILAEEDKSKNDEIVNKVRLAEEKYQDLETLHNKVRADFAKELQDKLVEKENEFKHLLVKQENQLILCCKNAEDLFETLANGLTENHKYKLNGLIVKIANLESCMHKLKEVTLKQRSRIYEQEKQLEQFSNELLSVNQSLNQATQEKEFATLSLKNEIEALNFQNEMLKKEGGSQQQVADEKESCITQLKKELSENINIVTSLTEVVKQKESEVSSLSALINELKLKHENCVDLTEKENAIAFLTAQQKQSQQELQNQIQDLMAKIEVLNKEKSAQIDEAAKLKNKLDEWKKKAELKFVQNHNTIKELKEKVESVNKQIVEKDEKLRTVTEEFECHRSNIQETQLMSESKEFELTTQLEGQKSKISELEDQCSKLMSEKKSLMDQIEIQTIQQSEDKKALVEQLQHIQYASDNNSKEVQNKVITLEKEIDALKEKLDAEQTKWQLEKAECFQLKDKELKILEEKLTAESTNKLSELKKKAEQKIASLKKQLMSQIDEKEQSRKALESQLNVLKDNMQKESQEVQEGLQQKVENQDKKYALLLEQKKNTEADLANIRSELKLKELRVKEMEDILKDVQQKHSERELTDSEKEQALLKELESQMLTNNQLVSTHEKALRALQDDLNVKDAEIKTFKEEIDEKSKSLNELQVLFEETQSQESAMRIKLEEMESEKLKFKNEITKLQKDMRSLRKEHSQELDLLKKEITEEAEQRIKYEQEDSELKHNSKLKQLMREFNTQMAQKEREVETAVQETIAKAQEVEAELMRSQQIEAVHLHKKIAEKEDDLKRTVKRYEELLEAREEEMTAKVTELQEQLERLQEQLTQKEMQNEEEENVEEATSPEVFQAQLAQKTSQVNEYKLKEQELKEKVHILEDQVKNFTRGVFYTPIGTPYKEVNNQHAGVSVFGEPTEFEYLRKVMFEYMMGRETKTMAKVITTVLRFPTDQAQQILQREETRPLSWLLSSS

Target ORF information:

RefSeq Version	XM_018094818.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis golgin A4 (golga4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094818.1

ORF Insert Sequence:

ATGTTTAAGA AACTGAAACA GAAGATCAGC GAAGAGCAGT CCCCTTCCAA AAGCCCCCTT 
GCTCCACAGC AGCAGGGTCG TCCAAGAAGC ACATCAACAG GCAACAGAAG TCGGACTTCG 
TCAGTAACAG AACAGCAGGA TGACAGCCTG GCAACAGCAG ATAGAGAGAA CGGATCATTG 
TCGGACAATA TTAATGGAAA TGATCCCACT TCTTCTCAGA GGAATGAACC TCAGTCTTTT 
GCACATAAGC TACAAATGCG GGTTCCTTCA ATGGAATCAC TGTTTCGAAG CCCCATTAAA 
GAATCACTTT TTCCTTCTTC AACTAAAGAA AATCTTTTGC GGAGTAGTTC ACGGGATTCC 
CTGAATAAAA TTGATATGGA GAATCCAAGT ACTGTATTTG ATCCCCCATC CGATATTGAA 
AGTGAAGCTG AGGATTCTGT GGGAAGTATA GAAGGACTTA ACAAAGAACA GCTGTTTAAT 
CGACTACATA GAATGGATAA AAGTTTAGGA AACTACAGAG GAAAATATTC TGAGCTTGTA 
ACAGCCTACA GAACAATGCA GAGAGACAAA GAGAAACTAC AGTCCATCTT GAGTCAGAGC 
CAGGACAAAG CACTAAGGAG AATTGGAGAG CTGCGAGAGG AGCTTCAAAT GGATCAACAG 
GCTAAAAAGC ATCTACAGGA AGAGTTTGAT GCATCTCTGG AAGAGAAAGA TCAACTTATC 
AGTGTTCTGC AAACACAGGT TAGTTTACTT AAAAACAGAC TTCAGAATGG ACAAAGCAGT 
ACTGAATTAC CAGAAAGCAC AGCCTCGCCA GAACTTGACT CTGAGAGTTC CTTGCAAGAG 
GTGGATACAG ATAACCGTTC CACCCCTGAT GATGGGAATG CTGCAAACAC TTTAAATGCT 
CTCCAGGTTA GAGTAAAACG TCAAGAGAAC CTTTTGCAAA GGTGTAAGAA ATCAATCAAG 
TCGCACAAGG AACAGTGTGC TCACCTCAGT AGTGAAAATG AAGCTCTTCA AAACCAACTA 
GATGAAAGAC TCCAGGAACT TGAGAAAATT AAAGAACTTC ACACTAGTGA GAAGAGCAAG 
CTGATTACCC AGCTTAGAGA TGCCAAGAAT TCAATTGAAC AGCTGGAACA AGATAAGGGT 
ATGATGATTG CAGAAACAAA ACGGCAGATG CATGAGACAT TAGAACTGAA GGAAGAAGAG 
GTTTCTCAGC TTCGGTCAAG GGTCAAGAAA TTAAGTTCAC ATTGTGAGGA ACTGCAGCAA 
CAGAAAGAAA AGTTAGAGAA AGCTGCATTT GAAGATGTAG AAAAAATATC TTCACAGAAA 
GCTGAGGATG CTTTTGAAAA GCTGCAGATG GAAGAGCAAA TTAAAGCTAT AGAAAAGGCC 
AGTGAGGAGG AGCGTGTCAA TCTTCAGAAG GAATTAAGTC GTGTGAAGCA AGAGGTGGTG 
GACATTATGA AGAAATCCTG TGATAGTCGT ATTAGTGAGA TTGAGAAAGT TCATGCAGAA 
GCCTTGCTGA ATAAGGAACA GGAGTATAAT GAACAGATTC GCCTGAAGGA ACAAGAATTC 
CAGGATCAAA TGAATGCTGT CAGGATTCTT GACCTTGAGA GTTCTGTTGC TGAAGAAAGT 
AAGTCCCAAG ATTTTGTAAG CCTGATTGAA ACAGAGAGAA ATAATCACAA TGCAGAAGTA 
GCTACCATCA AAGAAATGCA TAAGCAAGAT CTTGATGTCC TTGTACAGGA TCAAGAAAGG 
ATATGGTCAG AAAAGCTTGA GCTGCTTAAG AAAGAGAATC ATACTAAGAT TATTGAACTT 
AAAGAGAAGT ATCAGCGAGA TTATGATTCT CACTTGAAAG AGAAAGAGCA TAATTTCCAG 
GAGCATATTG AGCATATGAA TGAAAAAACA TTAGAAAAAC TAGATGTAAA ACAAACTGAA 
TTGGAAACCC TCTCAGCTGA ACTGTCTGAG GCTATAAAGG CACGGCAAGA GATTGAGCAG 
AAACTTACAG ACACAGAAAA TGAGATTAGC AAAATAAAAC TAGAGTATGA AGCCCAGATA 
AAAGAAGAAA GAGAAAAACA CAGTGAAGAA ATTGGTTCCA TTGAAAGAGA GAAGGAAATG 
CATACTCAGG GTGTTGAGAA AGACTTGAAG GAAAATATTA ACAACCTAAA ACTACTTCAA 
GATAGTAAAG AATGTGAGTT GGAAGAATTC AGAAAGGAAA ATCAAAAGCT AAAAGAAGCT 
GCTGAAAAGG CAGAAGCAGA CCATAAAGAA GCATTTGCTC TATTGGAAGC AGCCAGGCAT 
TCTCATGAGA TAAATACACA TGAGAAAGAA CAGGCTTATG AGCTAAAATT GAAGGAGCTT 
CAGGAGATGC TAAAAGGTAT GACGCAGGAG CGAAACGATC TTAAAGAGGT GACAGAAAAA 
GCAGAAACAC AGTTAAAGAA TATGCAGATC GAGCTGGATT CTAACAAGGC CCAAGTACAA 
AGCCTTACAA AGCAAATTCA TGAGGAAAGT ATGAATATGG CAGATAAACT TAACACTTTG 
GTACTAGAGT CTGAAACTCA GGTAAAAGGG TTGAAGGAAC AAATAGAAGA GATAAGCAGA 
ATAGCTTCAG AGCGTGAACA TGAACTTAAA AAAGTAAAAG AAGATCATAT TCAAATTATT 
TTAGACCTAA ACAATACTAT ATCTGAAAAA GAGAAGAATA TACTTTCTTT GCAAGAAGAT 
TACAAAGTAA AACACAAAAA TCTGGATGCT AAAATGGAAA AATTAAAGCA GAAGTTTAAA 
GAGCTGCAAG AATCATCTAA AAAGAAATAT TCAGAGTTGG AAAGCAAACT TAAGAAAGAG 
ATCGAGAAGA AGCAGAACGA GTTGATTAAC AAAGAAAGGG AGTTTAATGA AAAAATACAT 
GAGATGGCCC AAGCAAGTTC AACTGGAATT AATCATGCAA TGGCTCAGCT TGAACTGAAT 
CATAAAGAGC AGATTCAGAA TATGACAGAC AATCATCAGA GAGAATTACA GGAAACAACA 
GAAGCTTGTG AAAAGAAATT AAGCCAACTT GCTGAAGACC TTAGAGAGAA GCACGAAGAA 
GAACTTCAGG AGAAAGATCA GGTCATAGCA GAATCAAGAA AACAGTTTAA TATCAAGAAT 
AAAGAAAAAG AAGATGCAGA CAAAGAGATA TCAGACCTCA GGGAACTGAA GACCCAAATG 
GATGCCACGT TTAATGATTT GCAAGAACAG CTGTGTCAGT CAGCAGCTCA AGTTTTACAT 
TTGACACAGA GTGAAAATGA ATTCAAGACA AATTGTACTC GGCTAACAAC GGAGAAGCAA 
ATTCTTCAAC AACAGCTTGA TGAACTGAAA ATATTAGCAG AGGAAGATAA AAGCAAGAAT 
GATGAAATAG TTAACAAGGT GAGACTTGCT GAAGAAAAGT ATCAAGACTT GGAGACCCTG 
CACAATAAAG TGAGAGCAGA CTTTGCTAAG GAACTGCAGG ATAAACTTGT TGAAAAGGAA 
AATGAGTTTA AACACCTTTT GGTTAAACAA GAAAACCAGT TGATCTTGTG CTGCAAAAAT 
GCTGAAGATT TATTTGAAAC ACTTGCAAAT GGACTCACTG AAAACCACAA ATATAAGTTG 
AATGGATTGA TTGTTAAAAT AGCAAATCTT GAAAGCTGCA TGCATAAACT AAAAGAGGTC 
ACTTTAAAAC AGCGTAGTAG AATATATGAA CAAGAAAAAC AACTTGAGCA GTTTTCAAAC 
GAACTTCTTT CAGTAAACCA ATCTCTTAAT CAAGCAACTC AGGAGAAAGA ATTTGCTACT 
TTGTCGCTAA AGAATGAGAT TGAAGCTCTT AATTTTCAAA ATGAGATGTT AAAAAAAGAA 
GGAGGAAGTC AACAGCAGGT TGCAGATGAG AAAGAATCTT GCATTACTCA GCTGAAAAAA 
GAACTTTCAG AGAATATAAA TATTGTGACT TCCTTGACAG AAGTGGTAAA ACAGAAAGAA 
TCTGAGGTAT CTTCTCTTTC TGCCTTAATT AATGAACTTA AACTGAAACA TGAGAATTGT 
GTAGATTTAA CAGAAAAAGA AAATGCAATT GCTTTTCTCA CTGCACAGCA GAAACAGAGT 
CAGCAAGAGC TACAGAATCA AATTCAGGAT CTAATGGCAA AGATTGAGGT TCTGAATAAA 
GAAAAATCTG CACAAATAGA TGAGGCTGCT AAACTTAAAA ATAAGTTGGA TGAATGGAAG 
AAAAAGGCAG AACTTAAATT TGTGCAGAAC CACAACACTA TTAAAGAGCT AAAAGAGAAA 
GTTGAATCAG TCAATAAGCA AATTGTTGAA AAAGATGAAA AACTTAGGAC AGTGACAGAA 
GAATTTGAAT GTCATCGTAG CAATATCCAA GAAACACAAC TCATGAGTGA AAGCAAAGAA 
TTTGAATTAA CTACCCAGTT GGAAGGCCAG AAATCTAAAA TTTCGGAATT GGAGGACCAG 
TGTTCGAAGC TGATGTCTGA AAAGAAAAGT TTGATGGATC AAATCGAAAT TCAAACTATT 
CAGCAGAGTG AAGATAAGAA AGCACTGGTT GAGCAACTCC AGCATATTCA GTATGCTTCT 
GACAATAACT CCAAGGAAGT GCAAAATAAA GTTATAACAC TTGAAAAAGA AATTGATGCT 
TTAAAGGAGA AACTTGATGC AGAGCAAACA AAATGGCAGC TGGAGAAAGC AGAGTGCTTT 
CAGCTGAAAG ATAAGGAATT AAAAATATTG GAAGAGAAAC TAACTGCAGA AAGCACAAAT 
AAACTTTCAG AGCTCAAGAA AAAAGCTGAA CAGAAAATCG CTTCTCTTAA GAAACAACTT 
ATGTCTCAGA TAGATGAGAA GGAGCAGTCC AGGAAAGCGC TGGAAAGCCA GCTAAATGTC 
TTAAAAGATA ATATGCAGAA AGAGAGCCAG GAAGTGCAGG AAGGATTGCA ACAAAAAGTT 
GAAAATCAAG ACAAAAAATA TGCATTGCTA CTGGAGCAGA AGAAAAATAC AGAAGCAGAT 
TTAGCTAATA TTAGAAGTGA ACTAAAATTA AAGGAACTAA GAGTTAAAGA AATGGAAGAT 
ATTCTTAAAG ATGTCCAGCA AAAACATTCT GAAAGAGAGC TGACCGACAG TGAGAAAGAA 
CAAGCACTTT TAAAAGAACT AGAGTCACAG ATGCTGACAA ATAATCAGCT GGTTTCTACT 
CATGAAAAAG CTTTAAGAGC CCTGCAAGAT GACCTCAATG TCAAGGATGC AGAGATAAAG 
ACCTTTAAAG AGGAAATAGA TGAGAAATCC AAATCCTTAA ATGAACTCCA AGTGCTTTTT 
GAGGAGACTC AGTCTCAAGA GAGCGCAATG AGAATAAAAC TTGAGGAAAT GGAGAGTGAG 
AAGCTAAAAT TCAAGAATGA AATAACTAAG CTACAGAAAG ATATGCGCTC TTTACGTAAG 
GAACACAGCC AAGAACTAGA TTTGCTTAAA AAGGAGATAA CCGAGGAAGC CGAACAAAGA 
ATCAAATACG AACAGGAAGA CTCGGAACTT AAGCATAATT CTAAATTAAA GCAACTCATG 
AGAGAATTCA ATACCCAGAT GGCTCAAAAG GAGCGTGAGG TTGAAACAGC TGTTCAGGAG 
ACTATTGCTA AAGCTCAGGA GGTTGAGGCT GAATTAATGC GATCACAACA GATAGAAGCA 
GTCCATCTTC ATAAAAAGAT TGCTGAGAAA GAAGATGACC TAAAGAGAAC TGTAAAAAGA 
TATGAAGAAC TTTTAGAGGC CCGAGAAGAG GAAATGACAG CAAAAGTGAC AGAGTTACAA 
GAGCAACTTG AAAGATTACA GGAACAATTA ACTCAGAAGG AAATGCAAAA TGAAGAAGAA 
GAAAATGTGG AGGAGGCAAC ATCTCCTGAG GTTTTTCAGG CGCAGCTGGC ACAGAAAACA 
TCGCAGGTCA ATGAATACAA ACTGAAGGAG CAGGAACTTA AGGAGAAGGT TCACATCCTA 
GAAGACCAGG TGAAAAACTT TACTCGTGGT GTATTTTATA CTCCTATTGG CACACCGTAT 
AAAGAAGTTA ATAATCAACA TGCAGGGGTT TCTGTTTTTG GCGAACCCAC TGAATTTGAG 
TACTTAAGAA AGGTGATGTT TGAGTACATG ATGGGGCGTG AAACAAAGAC AATGGCAAAG 
GTAATAACAA CTGTGCTGAG GTTCCCAACA GATCAAGCTC AACAAATTTT GCAACGAGAA 
GAAACCCGAC CACTGTCTTG GCTTCTATCT TCCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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1-732-210-0262

golga4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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