thsd7a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	thsd7a
Entrez Gene ID	100495599
Full Name	thrombospondin type 1 domain containing 7A
General protein information	Preferred Names thrombospondin type 1 domain containing 7A Names thrombospondin type-1 domain-containing protein 7A thrombospondin, type I, domain containing 7A
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012965596.2	XP_012821050.1	thrombospondin type-1 domain-containing protein 7A isoform X2
XM_012965595.3	XP_012821049.1	thrombospondin type-1 domain-containing protein 7A isoform X1
XM_012965595.2	XP_012821049.1	thrombospondin type-1 domain-containing protein 7A isoform X1
XM_012965596.3	XP_012821050.1	thrombospondin type-1 domain-containing protein 7A isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	THSD7A	NP_056019.1
Canis lupus familiaris (dog)	THSD7A	XP_003431913.2
Bos taurus (cattle)	THSD7A	NP_001193672.1
Danio rerio (zebrafish)	thsd7a	NP_001116232.1
Mus musculus (house mouse)	Thsd7a	NP_001158277.1
Pan troglodytes (chimpanzee)	THSD7A	XP_527669.2
Rattus norvegicus (Norway rat)	Thsd7a	NP_001178899.1
Gallus gallus (chicken)	THSD7A	XP_418686.3
Xenopus tropicalis (tropical clawed frog)	thsd7a	XP_002939058.2

Gene Ontology

The following thsd7a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the thsd7a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa60481	XM_012965595.3 Latest version!	Xenopus tropicalis thrombospondin type 1 domain containing 7A (thsd7a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa60482	XM_012965596.3 Latest version!	Xenopus tropicalis thrombospondin type 1 domain containing 7A (thsd7a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa11988	XM_012965595.2 Latest version!	Xenopus tropicalis thrombospondin type 1 domain containing 7A (thsd7a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa36335	XM_012965596.2 Latest version!	Xenopus tropicalis thrombospondin type 1 domain containing 7A (thsd7a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

thsd7a ( XM_012965595.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821049.1 Xenopus tropicalis thrombospondin type 1 domain containing 7A (thsd7a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa60481
Clone ID Related Accession (Same CDS sequence)	XM_012965595.3
Accession Version	XM_012965595.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	thrombospondin type-1 domain-containing protein 7A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965595.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGAA GCACCGGCTT TTCTGGTACC CTTGCTTCTT CCCTGGGAGT GATTGTTCTT 
CTTCTCGGTG GGATCTGGGC TGCTGAGACG CAGAGTGAAT CTGAAACCAA AACTATATAC 
ATATGGAAGA CTGGTCCCTG GGGCAGATGT ATGGGCGATG AATGTGGACC TGGAGGTATT 
CAAACAAGGG CAGTTTGGTG TGCCCATGTC GAAGGATGGA CGACCCTCCC TACAAACTGT 
AAACAGACAG ACAGGCCCGA AAACCAACAG AACTGTTTTA GAGTTTGCGA CTGGCACAAG 
GAAATGTATG ACTGGCAGTT AGGAAGTTGG AATCAGTGTA AACCAGTTAT ATCCAGATCT 
TCAGAGAAAC CCACAGAATG TACTAAAGGT GAGGAAGGCA TTCAGACTAG AGAAGTAACA 
TGCATCCAGA AAGCAAATGG GGCAGTAGCT GATGATAGTA TATGTGAATA CTTTGAACCT 
AAGCCTCGAC TGGAACAAGG ATGCCTCATT CCCTGCCCTC AAGACTGCAT TGTATCAGAA 
TATTCTTCAT GGTCAGAATG CTCCAGAACA TGTGGCAATG GATTAAAACA CAGAACCCGA 
TATGTTATTA CTTTGGCCCA GTTTGGTGGA TCAGCATGTC CTAACCTTAC AGAATACCAA 
ACATGTCAGT CTGCCCCATG TGAGGGTGAA GAAAATATGT ACAGTCTGAG TGTTGGACCC 
TGGAGTTCTT GCTCAATTCC ACTTTCTAGA CATGTAAGAC AGACTGGTAA ACGAGGCAAA 
AATAGAGAGC GAGCGAAGGA AAGAGAAAAG GCAGTGAAGG ATCCAGAAGC ACGTGAGCTT 
ATTAAAAAGA AAAGAAACCG GAACAGAAAG AATAGACAGG AGAATAGATA TTGGGATATA 
CAGATTGGTT ACCAGACACG ACTAGTAACT TGTATGCACC GGAATGGAAA AACTGCTGCG 
TTAAGTTTTT GCCAACAGGA AAAGTTGCCA ATTACGTTCC AGTCTTGTGT TATTGACAAG 
GAGTGCCAAG TGTCTGAGTG GGCAGAATGG AGCCCTTGCT CCAGGGCTTG TGATATGGAA 
TCTCCTGGTG GATATCGTAC TAGGAAAAGG ACTATCAAAC TATTTCCAAT TGGGAATGGA 
AACGAATGTC CAGAGCTAGA AGAAACAGAA GATTGTACTC CTCCACCAGA CGGGCCACAA 
GTTTGTATTA AATACACCTG GAGAGCTACA GAATGGACTG AATGCCGTGT TGATCCTTTG 
TTAAGCCAGC AGGATAAAAG GAGAGGTAAC CAGACTGCTC TATGCGGCGG AGGAATTCAA 
ACACGGGAGA TTTACTGTGT ACAGGCAAAT GAAAATCTGC TTTCTTACCT CAACAATGTA 
AAAGATAAAG AAGAAAGCCA TTCTTACAGC ACATCAAAGT CTAGTGCGGC ACCATTTCTA 
TCAATCACTT TTGAAATATC AGCATCCAAG CCTGTTGATC CCAAATTGTG CTCGGAATCA 
CCACTAAGTA CAAGCCAGCT CTGCCATGTT CCATGCCCTA TTGAGTGTGA AGTTTCTCCA 
TGGTCAGCAT GGGGACCTTG CACCTTTGAA AACTGTGAAG ACCAACAAAG CAAAAAAGGG 
TTCAAGCTCA GAAAGCGCAA TATTACCAAT GAGCCTACTG GTGGGACTGG CAATTGCCCT 
CACCTTGTAG AAGCCATTCC TTGTGAAGAA CCATCTTGCT ACGACTGGCG ATTGGATAAA 
CTTGAAGAGT GTATTCCTGA CAACGGAAAC AATTGTGGGG CAGGAACTCA GGTGCCTGTT 
GTTACATGCA TTAATAGTGA CGGAGAAGAA GTTGACAGAC AACAGTGCAG AGATGCCATC 
TATCCGATCC CAATTTTGTG TGAAGTTCCC TGCCCAAAGG ATTGTGTTCT CAGCATGTGG 
TCTGAATGGT CGTCCTGCTC ACACACCTGC TCTGGAAAAA CAACAGAAGG ACGGCAGACA 
CGGACGCGGT CTATTCTCGC TTACGCAGGT GATGGAAGAA GTCCGTGCCC AGAAAGCAAC 
GCTCTGCAAG AGATTCGTAG CTGTAATGAG CATCCTTGTA CAGTATATCA CTGGCAGACG 
GGTCCCTGGG GCCAGTGCAC TGAGGATGTC ACTGTTTCAG GTTTCAATTC ATCCAACTGG 
AATGGTGAAG CTACTTGTGC TCTAGGGATG CAAACAAGAA AAATCATTTG TGTTCGAGTC 
AACGTGGGAC AGGTTGGACC CAAAAAATGT CCTGAAAGCC TTCGTCCAGA AACCATGAGG 
CCATGTCTGC TTCCATGTCG AAAGGATTGC ATAGTTACAC CATATAGTGA CTGGGCCACT 
TGCCCCACCG CCTGTCAAGA CGGTGATGTT GCCATAAAAA AACAGACTCG TCATCGTGTC 
ATTATCCAAC TCCCATCTTA TGGAGGCCGT GACTGCCCCG ATACTTTATA CGAAGAAAAT 
GAATGCGAAG CTCCAGCAAC CTGTCACAGC TACCGCTGGA AAACACATAA ATGGAGAAGA 
TGCCAGTTGG TGCCGTGGTA CGTCCGACAA GACAGCCCTG GAGCTTATGA AACGTGTGGG 
CCTGGATTAC AAGCCAGAGC AATTACTTGC CGCAGACAAG ATGGTGCACA GGTTGATGTC 
ACTGATTGTC TTGCCAAAGC TGGTCCAATG CCTCCTTTAA CCCAGACCTG CCAGATACCT 
TGCCAAGATG ACTGTCAATT TACTAACTGG TCGAAATTTT CTTCATGTAA TGGTGACTGT 
GGTGCAGTAA GGACAAGAAA GCGATCACTT CTTGGGAAAA GTAAGAAAAA AGACAAGTGC 
AAGAACGCCC AGCTCTACCC AGTGATTGAG ACTCAGTTCT GCCCATGTGA TAAATACAGT 
GCTCAGCCTG TGGGAAACTG GTCAGACTGC ATCTTGCCAG AAGGAAAAGT GGAGGCCTTA 
CTGGGGGTAA AAGTACAAGG AGACATCAAG GAGTGTGGGC AGGGCTATCG TTACCAAGCC 
ATGGCATGTT ATGATCAAAA CGGCAGACTT GTGGAGACTT CAAGGTGTAA CAGCCATGGT 
TATATTGAGG AAGCATGTAT CATACCCTGT CCATCTGATT GTAAGCTTAG CGAATGGTCC 
AACTGGTCAC GGTGTAGCAA GTCCTGTGGG AGTGGAGTCA AGGTTCGGTC GAAATGGCTC 
CGTGAAAAAC CTTATAATGG TGGAAGACCT TGTCCTAAAC TGGATCACGT AAACCAGGCT 
CAGGTATATG AAGTGGTTCC ATGTCACAGT GATTGCAATC AGTATATATG GGTCACAGAG 
CCTTGGAGTA TTTGCAAAGT GACTAATGTT GATCTAAAGC AGAACTGCGG CGAAGGTGTT 
CAGACAAGAA AAGTAAGATG CATGCAGAAC ACAGTAGATG GCCCTTCGGA TGTTGTGGAA 
GACTATCTAT GTGAACCCGA GGAAATGCCA TTGGGCGCCA GAAACTGCAA GCTGCCCTGT 
CCTGAAGACT GTGTGATGTC TGAATGGAGT GAATGGAGCA GGTGTCCCTT GCCCTGTGAC 
GGAAGCAGCT ACCGAGAGAG GTCAGCCCTA CCTATCCGAC AAGCCGAGGA AGGCAAAGCC 
TGCCATGTTG CAATTGAAAC GGAGCCCTGC AACCTGAATA AAAACTGTTA TCACTATGAA 
TACAATGTCA CGGACTGGAG CACTTGCCAG CTAAGTGAGA AGGCAGTTTG TGGAAACGGG 
ATAAAAACAA GAATGCTGGA CTGCGTCCGC AGCGATGTCA AATCAGTTGA CTTGAAATAC 
TGTGAAGAGC TTGGCTTGGA AAAGACATGG CAGATGAATA TTTCTTGTGT GGTGGAATGT 
CCTATCAACT GTCAACTCTC CGAATGGTCA CCATGGTCCA AATGTTCCAA AGACTGTGGC 
CTAGCAGGTA AAATGTTACG GAATAGAACA GTGATTCAGC CGTCCCAGGG TGATGGAAGA 
CCTTGCCCTT CACAGATGGA GCAATCCAAA CCCTGCCCAG TAAAGCCATG TTACCGGTGG 
CAGTATGGAC GGTGGTCTTT GTGCAAAGTT GAGGATGCTC AGTGTGGTGT AGGAACAAGG 
TTCAGAAACA TTTCTTGTGT AGTGCATGAT GGATCCAGGG AAGATTCTGG TAAAATAGTA 
GACGAGGAGT TTTGCGGAGA CATTGACCTT ACTGTAAATA ATGACAAGAA CATTGTGCTA 
GAGGAATCAT GCACCGTTCC ATGCCCAGGT GATTGTTACT TGAGCGAATG GTCTGAGTGG 
AGTTTGTGTC AGCTGACTTG TGTTAATGGA GAAGATCTTG GCTTTGGAGG AATAAAGATG 
CGATCGCGTG CAGTAATTCT TCAAGAAATG GAAAATCAAC ATCTGTGTCC TGAACAAGTT 
GTGGAATCAC AACCATGTAA TGATGGAAAG TGTTATGAAT ATAAATGGCT GTCAAGTCCT 
TGGAAAGGAT CTTCTCGAAT GGTCTGGTGT CAGAGGTCAG ATGGCTTAAA TGTTACAGGA 
GGCTGTTTAG AGGCTTATCA TCCAGAAACT GAAAGATCGT GTAATCCACC TTGCACGCAG 
CAACACTCAT ATTGCACCGA GACAGGGTCA TGTGGCTGTG AAGATGGATA TACTGAAGTG 
ACATCGTCTG ATGGAACACT AGATCAGTGT ACTCTGATCC CAGTGGTGAT AATGCCAACA 
TTAGATGAAA AAAAGGGAGA CGTTAAAACG AGTCGTGCCA TCAACCCAAC CCAGCCATCC 
AGTAGTCAGT CGGGTTATGG AGGAAGAACA TGGTTTTTAC AACCCTTCGG TTCAGACGGT 
AGACTGAAGA CCTGGGTGTA TGGGGTAGCA GCTGGAGCCT TTGTGTTACT CATCTTTGTT 
GTTTCTATGA TCTATTTGGC CTGCAAAAAG CCGAAGAAAC CCCCGAGACG ACAGAACAAC 
CGCCTGAAAC CCTTAACTCT GGCCTACGAT GGAGATGCAG ACATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821049.1
CDS	341..5308
Translation	MGRSTGFSGTLASSLGVIVLLLGGIWAAETQSESETKTIYIWKTGPWGRCMGDECGPGGIQTRAVWCAHVEGWTTLPTNCKQTDRPENQQNCFRVCDWHKEMYDWQLGSWNQCKPVISRSSEKPTECTKGEEGIQTREVTCIQKANGAVADDSICEYFEPKPRLEQGCLIPCPQDCIVSEYSSWSECSRTCGNGLKHRTRYVITLAQFGGSACPNLTEYQTCQSAPCEGEENMYSLSVGPWSSCSIPLSRHVRQTGKRGKNRERAKEREKAVKDPEARELIKKKRNRNRKNRQENRYWDIQIGYQTRLVTCMHRNGKTAALSFCQQEKLPITFQSCVIDKECQVSEWAEWSPCSRACDMESPGGYRTRKRTIKLFPIGNGNECPELEETEDCTPPPDGPQVCIKYTWRATEWTECRVDPLLSQQDKRRGNQTALCGGGIQTREIYCVQANENLLSYLNNVKDKEESHSYSTSKSSAAPFLSITFEISASKPVDPKLCSESPLSTSQLCHVPCPIECEVSPWSAWGPCTFENCEDQQSKKGFKLRKRNITNEPTGGTGNCPHLVEAIPCEEPSCYDWRLDKLEECIPDNGNNCGAGTQVPVVTCINSDGEEVDRQQCRDAIYPIPILCEVPCPKDCVLSMWSEWSSCSHTCSGKTTEGRQTRTRSILAYAGDGRSPCPESNALQEIRSCNEHPCTVYHWQTGPWGQCTEDVTVSGFNSSNWNGEATCALGMQTRKIICVRVNVGQVGPKKCPESLRPETMRPCLLPCRKDCIVTPYSDWATCPTACQDGDVAIKKQTRHRVIIQLPSYGGRDCPDTLYEENECEAPATCHSYRWKTHKWRRCQLVPWYVRQDSPGAYETCGPGLQARAITCRRQDGAQVDVTDCLAKAGPMPPLTQTCQIPCQDDCQFTNWSKFSSCNGDCGAVRTRKRSLLGKSKKKDKCKNAQLYPVIETQFCPCDKYSAQPVGNWSDCILPEGKVEALLGVKVQGDIKECGQGYRYQAMACYDQNGRLVETSRCNSHGYIEEACIIPCPSDCKLSEWSNWSRCSKSCGSGVKVRSKWLREKPYNGGRPCPKLDHVNQAQVYEVVPCHSDCNQYIWVTEPWSICKVTNVDLKQNCGEGVQTRKVRCMQNTVDGPSDVVEDYLCEPEEMPLGARNCKLPCPEDCVMSEWSEWSRCPLPCDGSSYRERSALPIRQAEEGKACHVAIETEPCNLNKNCYHYEYNVTDWSTCQLSEKAVCGNGIKTRMLDCVRSDVKSVDLKYCEELGLEKTWQMNISCVVECPINCQLSEWSPWSKCSKDCGLAGKMLRNRTVIQPSQGDGRPCPSQMEQSKPCPVKPCYRWQYGRWSLCKVEDAQCGVGTRFRNISCVVHDGSREDSGKIVDEEFCGDIDLTVNNDKNIVLEESCTVPCPGDCYLSEWSEWSLCQLTCVNGEDLGFGGIKMRSRAVILQEMENQHLCPEQVVESQPCNDGKCYEYKWLSSPWKGSSRMVWCQRSDGLNVTGGCLEAYHPETERSCNPPCTQQHSYCTETGSCGCEDGYTEVTSSDGTLDQCTLIPVVIMPTLDEKKGDVKTSRAINPTQPSSSQSGYGGRTWFLQPFGSDGRLKTWVYGVAAGAFVLLIFVVSMIYLACKKPKKPPRRQNNRLKPLTLAYDGDADM

Target ORF information:

RefSeq Version	XM_012965595.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis thrombospondin type 1 domain containing 7A (thsd7a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965595.3

ORF Insert Sequence:

ATGGGGAGAA GCACCGGCTT TTCTGGTACC CTTGCTTCTT CCCTGGGAGT GATTGTTCTT 
CTTCTCGGTG GGATCTGGGC TGCTGAGACG CAGAGTGAAT CTGAAACCAA AACTATATAC 
ATATGGAAGA CTGGTCCCTG GGGCAGATGT ATGGGCGATG AATGTGGACC TGGAGGTATT 
CAAACAAGGG CAGTTTGGTG TGCCCATGTC GAAGGATGGA CGACCCTCCC TACAAACTGT 
AAACAGACAG ACAGGCCCGA AAACCAACAG AACTGTTTTA GAGTTTGCGA CTGGCACAAG 
GAAATGTATG ACTGGCAGTT AGGAAGTTGG AATCAGTGTA AACCAGTTAT ATCCAGATCT 
TCAGAGAAAC CCACAGAATG TACTAAAGGT GAGGAAGGCA TTCAGACTAG AGAAGTAACA 
TGCATCCAGA AAGCAAATGG GGCAGTAGCT GATGATAGTA TATGTGAATA CTTTGAACCT 
AAGCCTCGAC TGGAACAAGG ATGCCTCATT CCCTGCCCTC AAGACTGCAT TGTATCAGAA 
TATTCTTCAT GGTCAGAATG CTCCAGAACA TGTGGCAATG GATTAAAACA CAGAACCCGA 
TATGTTATTA CTTTGGCCCA GTTTGGTGGA TCAGCATGTC CTAACCTTAC AGAATACCAA 
ACATGTCAGT CTGCCCCATG TGAGGGTGAA GAAAATATGT ACAGTCTGAG TGTTGGACCC 
TGGAGTTCTT GCTCAATTCC ACTTTCTAGA CATGTAAGAC AGACTGGTAA ACGAGGCAAA 
AATAGAGAGC GAGCGAAGGA AAGAGAAAAG GCAGTGAAGG ATCCAGAAGC ACGTGAGCTT 
ATTAAAAAGA AAAGAAACCG GAACAGAAAG AATAGACAGG AGAATAGATA TTGGGATATA 
CAGATTGGTT ACCAGACACG ACTAGTAACT TGTATGCACC GGAATGGAAA AACTGCTGCG 
TTAAGTTTTT GCCAACAGGA AAAGTTGCCA ATTACGTTCC AGTCTTGTGT TATTGACAAG 
GAGTGCCAAG TGTCTGAGTG GGCAGAATGG AGCCCTTGCT CCAGGGCTTG TGATATGGAA 
TCTCCTGGTG GATATCGTAC TAGGAAAAGG ACTATCAAAC TATTTCCAAT TGGGAATGGA 
AACGAATGTC CAGAGCTAGA AGAAACAGAA GATTGTACTC CTCCACCAGA CGGGCCACAA 
GTTTGTATTA AATACACCTG GAGAGCTACA GAATGGACTG AATGCCGTGT TGATCCTTTG 
TTAAGCCAGC AGGATAAAAG GAGAGGTAAC CAGACTGCTC TATGCGGCGG AGGAATTCAA 
ACACGGGAGA TTTACTGTGT ACAGGCAAAT GAAAATCTGC TTTCTTACCT CAACAATGTA 
AAAGATAAAG AAGAAAGCCA TTCTTACAGC ACATCAAAGT CTAGTGCGGC ACCATTTCTA 
TCAATCACTT TTGAAATATC AGCATCCAAG CCTGTTGATC CCAAATTGTG CTCGGAATCA 
CCACTAAGTA CAAGCCAGCT CTGCCATGTT CCATGCCCTA TTGAGTGTGA AGTTTCTCCA 
TGGTCAGCAT GGGGACCTTG CACCTTTGAA AACTGTGAAG ACCAACAAAG CAAAAAAGGG 
TTCAAGCTCA GAAAGCGCAA TATTACCAAT GAGCCTACTG GTGGGACTGG CAATTGCCCT 
CACCTTGTAG AAGCCATTCC TTGTGAAGAA CCATCTTGCT ACGACTGGCG ATTGGATAAA 
CTTGAAGAGT GTATTCCTGA CAACGGAAAC AATTGTGGGG CAGGAACTCA GGTGCCTGTT 
GTTACATGCA TTAATAGTGA CGGAGAAGAA GTTGACAGAC AACAGTGCAG AGATGCCATC 
TATCCGATCC CAATTTTGTG TGAAGTTCCC TGCCCAAAGG ATTGTGTTCT CAGCATGTGG 
TCTGAATGGT CGTCCTGCTC ACACACCTGC TCTGGAAAAA CAACAGAAGG ACGGCAGACA 
CGGACGCGGT CTATTCTCGC TTACGCAGGT GATGGAAGAA GTCCGTGCCC AGAAAGCAAC 
GCTCTGCAAG AGATTCGTAG CTGTAATGAG CATCCTTGTA CAGTATATCA CTGGCAGACG 
GGTCCCTGGG GCCAGTGCAC TGAGGATGTC ACTGTTTCAG GTTTCAATTC ATCCAACTGG 
AATGGTGAAG CTACTTGTGC TCTAGGGATG CAAACAAGAA AAATCATTTG TGTTCGAGTC 
AACGTGGGAC AGGTTGGACC CAAAAAATGT CCTGAAAGCC TTCGTCCAGA AACCATGAGG 
CCATGTCTGC TTCCATGTCG AAAGGATTGC ATAGTTACAC CATATAGTGA CTGGGCCACT 
TGCCCCACCG CCTGTCAAGA CGGTGATGTT GCCATAAAAA AACAGACTCG TCATCGTGTC 
ATTATCCAAC TCCCATCTTA TGGAGGCCGT GACTGCCCCG ATACTTTATA CGAAGAAAAT 
GAATGCGAAG CTCCAGCAAC CTGTCACAGC TACCGCTGGA AAACACATAA ATGGAGAAGA 
TGCCAGTTGG TGCCGTGGTA CGTCCGACAA GACAGCCCTG GAGCTTATGA AACGTGTGGG 
CCTGGATTAC AAGCCAGAGC AATTACTTGC CGCAGACAAG ATGGTGCACA GGTTGATGTC 
ACTGATTGTC TTGCCAAAGC TGGTCCAATG CCTCCTTTAA CCCAGACCTG CCAGATACCT 
TGCCAAGATG ACTGTCAATT TACTAACTGG TCGAAATTTT CTTCATGTAA TGGTGACTGT 
GGTGCAGTAA GGACAAGAAA GCGATCACTT CTTGGGAAAA GTAAGAAAAA AGACAAGTGC 
AAGAACGCCC AGCTCTACCC AGTGATTGAG ACTCAGTTCT GCCCATGTGA TAAATACAGT 
GCTCAGCCTG TGGGAAACTG GTCAGACTGC ATCTTGCCAG AAGGAAAAGT GGAGGCCTTA 
CTGGGGGTAA AAGTACAAGG AGACATCAAG GAGTGTGGGC AGGGCTATCG TTACCAAGCC 
ATGGCATGTT ATGATCAAAA CGGCAGACTT GTGGAGACTT CAAGGTGTAA CAGCCATGGT 
TATATTGAGG AAGCATGTAT CATACCCTGT CCATCTGATT GTAAGCTTAG CGAATGGTCC 
AACTGGTCAC GGTGTAGCAA GTCCTGTGGG AGTGGAGTCA AGGTTCGGTC GAAATGGCTC 
CGTGAAAAAC CTTATAATGG TGGAAGACCT TGTCCTAAAC TGGATCACGT AAACCAGGCT 
CAGGTATATG AAGTGGTTCC ATGTCACAGT GATTGCAATC AGTATATATG GGTCACAGAG 
CCTTGGAGTA TTTGCAAAGT GACTAATGTT GATCTAAAGC AGAACTGCGG CGAAGGTGTT 
CAGACAAGAA AAGTAAGATG CATGCAGAAC ACAGTAGATG GCCCTTCGGA TGTTGTGGAA 
GACTATCTAT GTGAACCCGA GGAAATGCCA TTGGGCGCCA GAAACTGCAA GCTGCCCTGT 
CCTGAAGACT GTGTGATGTC TGAATGGAGT GAATGGAGCA GGTGTCCCTT GCCCTGTGAC 
GGAAGCAGCT ACCGAGAGAG GTCAGCCCTA CCTATCCGAC AAGCCGAGGA AGGCAAAGCC 
TGCCATGTTG CAATTGAAAC GGAGCCCTGC AACCTGAATA AAAACTGTTA TCACTATGAA 
TACAATGTCA CGGACTGGAG CACTTGCCAG CTAAGTGAGA AGGCAGTTTG TGGAAACGGG 
ATAAAAACAA GAATGCTGGA CTGCGTCCGC AGCGATGTCA AATCAGTTGA CTTGAAATAC 
TGTGAAGAGC TTGGCTTGGA AAAGACATGG CAGATGAATA TTTCTTGTGT GGTGGAATGT 
CCTATCAACT GTCAACTCTC CGAATGGTCA CCATGGTCCA AATGTTCCAA AGACTGTGGC 
CTAGCAGGTA AAATGTTACG GAATAGAACA GTGATTCAGC CGTCCCAGGG TGATGGAAGA 
CCTTGCCCTT CACAGATGGA GCAATCCAAA CCCTGCCCAG TAAAGCCATG TTACCGGTGG 
CAGTATGGAC GGTGGTCTTT GTGCAAAGTT GAGGATGCTC AGTGTGGTGT AGGAACAAGG 
TTCAGAAACA TTTCTTGTGT AGTGCATGAT GGATCCAGGG AAGATTCTGG TAAAATAGTA 
GACGAGGAGT TTTGCGGAGA CATTGACCTT ACTGTAAATA ATGACAAGAA CATTGTGCTA 
GAGGAATCAT GCACCGTTCC ATGCCCAGGT GATTGTTACT TGAGCGAATG GTCTGAGTGG 
AGTTTGTGTC AGCTGACTTG TGTTAATGGA GAAGATCTTG GCTTTGGAGG AATAAAGATG 
CGATCGCGTG CAGTAATTCT TCAAGAAATG GAAAATCAAC ATCTGTGTCC TGAACAAGTT 
GTGGAATCAC AACCATGTAA TGATGGAAAG TGTTATGAAT ATAAATGGCT GTCAAGTCCT 
TGGAAAGGAT CTTCTCGAAT GGTCTGGTGT CAGAGGTCAG ATGGCTTAAA TGTTACAGGA 
GGCTGTTTAG AGGCTTATCA TCCAGAAACT GAAAGATCGT GTAATCCACC TTGCACGCAG 
CAACACTCAT ATTGCACCGA GACAGGGTCA TGTGGCTGTG AAGATGGATA TACTGAAGTG 
ACATCGTCTG ATGGAACACT AGATCAGTGT ACTCTGATCC CAGTGGTGAT AATGCCAACA 
TTAGATGAAA AAAAGGGAGA CGTTAAAACG AGTCGTGCCA TCAACCCAAC CCAGCCATCC 
AGTAGTCAGT CGGGTTATGG AGGAAGAACA TGGTTTTTAC AACCCTTCGG TTCAGACGGT 
AGACTGAAGA CCTGGGTGTA TGGGGTAGCA GCTGGAGCCT TTGTGTTACT CATCTTTGTT 
GTTTCTATGA TCTATTTGGC CTGCAAAAAG CCGAAGAAAC CCCCGAGACG ACAGAACAAC 
CGCCTGAAAC CCTTAACTCT GGCCTACGAT GGAGATGCAG ACATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

thsd7a ( XM_012965596.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821050.1 Xenopus tropicalis thrombospondin type 1 domain containing 7A (thsd7a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa60482
Clone ID Related Accession (Same CDS sequence)	XM_012965596.3
Accession Version	XM_012965596.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	thrombospondin type-1 domain-containing protein 7A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965596.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGAA GCACCGGCTT TTCTGGTACC CTTGCTTCTT CCCTGGGAGT GATTGTTCTT 
CTTCTCGGTG GGATCTGGGC TGCTGAGACG CAGAGTGAAT CTGAAACCAA AACTATATAC 
ATATGGAAGA CTGGTCCCTG GGGCAGATGT ATGGGCGATG AATGTGGACC TGGAGGTATT 
CAAACAAGGG CAGTTTGGTG TGCCCATGTC GAAGGATGGA CGACCCTCCC TACAAACTGT 
AAACAGACAG ACAGGCCCGA AAACCAACAG AACTGTTTTA GAGTTTGCGA CTGGCACAAG 
GAAATGTATG ACTGGCAGTT AGGAAGTTGG AATCAGTGTA AACCAGTTAT ATCCAGATCT 
TCAGAGAAAC CCACAGAATG TACTAAAGGT GAGGAAGGCA TTCAGACTAG AGAAGTAACA 
TGCATCCAGA AAGCAAATGG GGCAGTAGCT GATGATAGTA TATGTGAATA CTTTGAACCT 
AAGCCTCGAC TGGAACAAGG ATGCCTCATT CCCTGCCCTC AAGACTGCAT TGTATCAGAA 
TATTCTTCAT GGTCAGAATG CTCCAGAACA TGTGGCAATG GATTAAAACA CAGAACCCGA 
TATGTTATTA CTTTGGCCCA GTTTGGTGGA TCAGCATGTC CTAACCTTAC AGAATACCAA 
ACATGTCAGT CTGCCCCATG TGAGGGTGAA GAAAATATGT ACAGTCTGAG TGTTGGACCC 
TGGAGTTCTT GCTCAATTCC ACTTTCTAGA CATGTAAGAC AGACTGGTAA ACGAGGCAAA 
AATAGAGAGC GAGCGAAGGA AAGAGAAAAG GCAGTGAAGG ATCCAGAAGC ACGTGAGCTT 
ATTAAAAAGA AAAGAAACCG GAACAGAAAG AATAGACAGG AGAATAGATA TTGGGATATA 
CAGATTGGTT ACCAGACACG ACTAGTAACT TGTATGCACC GGAATGGAAA AACTGCTGCG 
TTAAGTTTTT GCCAACAGGA AAAGTTGCCA ATTACGTTCC AGTCTTGTGT TATTGACAAG 
GAGTGCCAAG TGTCTGAGTG GGCAGAATGG AGCCCTTGCT CCAGGGCTTG TGATATGGAA 
TCTCCTGGTG GATATCGTAC TAGGAAAAGG ACTATCAAAC TATTTCCAAT TGGGAATGGA 
AACGAATGTC CAGAGCTAGA AGAAACAGAA GATTGTACTC CTCCACCAGA CGGGCCACAA 
GTTTGTATTA AATACACCTG GAGAGCTACA GAATGGACTG AATGCCGTGT TGATCCTTTG 
TTAAGCCAGC AGGATAAAAG GAGAGGTAAC CAGACTGCTC TATGCGGCGG AGGAATTCAA 
ACACGGGAGA TTTACTGTGT ACAGGCAAAT GAAAATCTGC TTTCTTACCT CAACAATGTA 
AAAGATAAAG AAGAAAGCCA TTCTTACAGC ACATCAAAGT CTAGTGCGGC ACCATTTCTA 
TCAATCACTT TTGAAATATC AGCATCCAAG CCTGTTGATC CCAAATTGTG CTCGGAATCA 
CCACTAAGTA CAAGCCAGCT CTGCCATGTT CCATGCCCTA TTGAGTGTGA AGTTTCTCCA 
TGGTCAGCAT GGGGACCTTG CACCTTTGAA AACTGTGAAG ACCAACAAAG CAAAAAAGGG 
TTCAAGCTCA GAAAGCGCAA TATTACCAAT GAGCCTACTG GTGGGACTGG CAATTGCCCT 
CACCTTGTAG AAGCCATTCC TTGTGAAGAA CCATCTTGCT ACGACTGGCG ATTGGATAAA 
CTTGAAGAGT GTATTCCTGA CAACGGAAAC AATTGTGGGG CAGGAACTCA GGTGCCTGTT 
GTTACATGCA TTAATAGTGA CGGAGAAGAA GTTGACAGAC AACAGTGCAG AGATGCCATC 
TATCCGATCC CAATTTTGTG TGAAGTTCCC TGCCCAAAGG ATTGTGTTCT CAGCATGTGG 
TCTGAATGGT CGTCCTGCTC ACACACCTGC TCTGGAAAAA CAACAGAAGG ACGGCAGACA 
CGGACGCGGT CTATTCTCGC TTACGCAGGT GATGGAAGAA GTCCGTGCCC AGAAAGCAAC 
GCTCTGCAAG AGATTCGTAG CTGTAATGAG CATCCTTGTA CAGTATATCA CTGGCAGACG 
GGTCCCTGGG GCCAGTGCAC TGAGGATGTC ACTGTTTCAG GTTTCAATTC ATCCAACTGG 
AATGGTGAAG CTACTTGTGC TCTAGGGATG CAAACAAGAA AAATCATTTG TGTTCGAGTC 
AACGTGGGAC AGGTTGGACC CAAAAAATGT CCTGAAAGCC TTCGTCCAGA AACCATGAGG 
CCATGTCTGC TTCCATGTCG AAAGGATTGC ATAGTTACAC CATATAGTGA CTGGGCCACT 
TGCCCCACCG CCTGTCAAGA CGGTGATGTT GCCATAAAAA AACAGACTCG TCATCGTGTC 
ATTATCCAAC TCCCATCTTA TGGAGGCCGT GACTGCCCCG ATACTTTATA CGAAGAAAAT 
GAATGCGAAG CTCCAGCAAC CTGTCACAGC TACCGCTGGA AAACACATAA ATGGAGAAGA 
TGCCAGTTGG TGCCGTGGTA CGTCCGACAA GACAGCCCTG GAGCTTATGA AACGTGTGGG 
CCTGGATTAC AAGCCAGAGC AATTACTTGC CGCAGACAAG ATGGTGCACA GGTTGATGTC 
ACTGATTGTC TTGCCAAAGC TGGTCCAATG CCTCCTTTAA CCCAGACCTG CCAGATACCT 
TGCCAAGATG ACTGTCAATT TACTAACTGG TCGAAATTTT CTTCATGTAA TGGTGACTGT 
GGTGCAGTAA GGACAAGAAA GCGATCACTT CTTGGGAAAA GTAAGAAAAA AGACAAGTGC 
AAGAACGCCC AGCTCTACCC AGTGATTGAG ACTCAGTTCT GCCCATGTGA TAAATACAGT 
GCTCAGCCTG TGGGAAACTG GTCAGACTGC ATCTTGCCAG AAGGAAAAGT GGAGGCCTTA 
CTGGGGGTAA AAGTACAAGG AGACATCAAG GAGTGTGGGC AGGGCTATCG TTACCAAGCC 
ATGGCATGTT ATGATCAAAA CGGCAGACTT GTGGAGACTT CAAGGTGTAA CAGCCATGGT 
TATATTGAGG AAGCATGTAT CATACCCTGT CCATCTGATT GTAAGCTTAG CGAATGGTCC 
AACTGGTCAC GGTGTAGCAA GTCCTGTGGG AGTGGAGTCA AGGTTCGGTC GAAATGGCTC 
CGTGAAAAAC CTTATAATGG TGGAAGACCT TGTCCTAAAC TGGATCACGT AAACCAGGCT 
CAGGTATATG AAGTGGTTCC ATGTCACAGT GATTGCAATC AGTATATATG GGTCACAGAG 
CCTTGGAGTA TTTGCAAAGT GACTAATGTT GATCTAAAGC AGAACTGCGG CGAAGGTGTT 
CAGACAAGAA AAGTAAGATG CATGCAGAAC ACAGTAGATG GCCCTTCGGA TGTTGTGGAA 
GACTATCTAT GTGAACCCGA GGAAATGCCA TTGGGCGCCA GAAACTGCAA GCTGCCCTGT 
CCTGAAGACT GTGTGATGTC TGAATGGAGT GAATGGAGCA GGTGTCCCTT GCCCTGTGAC 
GGAAGCAGCT ACCGAGAGAG GTCAGCCCTA CCTATCCGAC AAGCCGAGGA AGGCAAAGCC 
TGCCATGTTG CAATTGAAAC GGAGCCCTGC AACCTGAATA AAAACTGTTA TCACTATGAA 
TACAATGTCA CGGACTGGAG CACTTGCCAG CTAAGTGAGA AGGCAGTTTG TGGAAACGGG 
ATAAAAACAA GAATGCTGGA CTGCGTCCGC AGCGATGTCA AATCAGTTGA CTTGAAATAC 
TGTGAAGAGC TTGGCTTGGA AAAGACATGG CAGATGAATA TTTCTTGTGT GGTGGAATGT 
CCTATCAACT GTCAACTCTC CGAATGGTCA CCATGGTCCA AATGTTCCAA AGACTGTGGC 
CTAGCAGGTA AAATGTTACG GAATAGAACA GTGATTCAGC CGTCCCAGGG TGATGGAAGA 
CCTTGCCCTT CACAGATGGA GCAATCCAAA CCCTGCCCAG TAAAGCCATG TTACCGGTGG 
CAGTATGGAC GGTGGTCTTT GTGCAAAGTT GAGGATGCTC AGTGTGGTGT AGGAACAAGG 
TTCAGAAACA TTTCTTGTGT AGTGCATGAT GGATCCAGGG AAGATTCTGG TAAAATAGTA 
GACGAGGAGT TTTGCGGAGA CATTGACCTT ACTGTAAATA ATGACAAGAA CATTGTGCTA 
GAGGAATCAT GCACCGTTCC ATGCCCAGGT GATTGTTACT TGAGCGAATG GTCTGAGTGG 
AGTTTGTGTC AGCTGACTTG TGTTAATGGA GAAGATCTTG GCTTTGGAGG AATAAAGATG 
CGATCGCGTG CAGTAATTCT TCAAGAAATG GAAAATCAAC ATCTGTGTCC TGAACAAGTT 
GTGGAATCAC AACCATGTAA TGATGGAAAG TGTTATGAAT ATAAATGGCT GTCAAGTCCT 
TGGAAAGGAT CTTCTCGAAT GGTCTGGTGT CAGAGGTCAG ATGGCTTAAA TGTTACAGGA 
GGCTGTTTAG AGGCTTATCA TCCAGAAACT GAAAGATCGT GTAATCCACC TTGCACGCAG 
CAACACTCAT ATTGCACCGA GACAGGGTCA TGTGGCTGTG AAGATGGATA TACTGAAGTG 
ACATCGTCTG ATGGAACACT AGATCAGTGT ACTCTGATCC CAGTGGTGAT AATGCCAACA 
TTAGATGAAA AAAAGGGAGA CGTTAAAACG AGTCGTGCCA TCAACCCAAC CCAGCCATCC 
AGTAGTCAGT CGGGTTATGG AGGAAGAACA TGGTTTTTAC AACCCTTCGG TTCAGCAAAA 
AGCCGAAGAA ACCCCCGAGA CGACAGAACA ACCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821050.1
CDS	341..5179
Translation	MGRSTGFSGTLASSLGVIVLLLGGIWAAETQSESETKTIYIWKTGPWGRCMGDECGPGGIQTRAVWCAHVEGWTTLPTNCKQTDRPENQQNCFRVCDWHKEMYDWQLGSWNQCKPVISRSSEKPTECTKGEEGIQTREVTCIQKANGAVADDSICEYFEPKPRLEQGCLIPCPQDCIVSEYSSWSECSRTCGNGLKHRTRYVITLAQFGGSACPNLTEYQTCQSAPCEGEENMYSLSVGPWSSCSIPLSRHVRQTGKRGKNRERAKEREKAVKDPEARELIKKKRNRNRKNRQENRYWDIQIGYQTRLVTCMHRNGKTAALSFCQQEKLPITFQSCVIDKECQVSEWAEWSPCSRACDMESPGGYRTRKRTIKLFPIGNGNECPELEETEDCTPPPDGPQVCIKYTWRATEWTECRVDPLLSQQDKRRGNQTALCGGGIQTREIYCVQANENLLSYLNNVKDKEESHSYSTSKSSAAPFLSITFEISASKPVDPKLCSESPLSTSQLCHVPCPIECEVSPWSAWGPCTFENCEDQQSKKGFKLRKRNITNEPTGGTGNCPHLVEAIPCEEPSCYDWRLDKLEECIPDNGNNCGAGTQVPVVTCINSDGEEVDRQQCRDAIYPIPILCEVPCPKDCVLSMWSEWSSCSHTCSGKTTEGRQTRTRSILAYAGDGRSPCPESNALQEIRSCNEHPCTVYHWQTGPWGQCTEDVTVSGFNSSNWNGEATCALGMQTRKIICVRVNVGQVGPKKCPESLRPETMRPCLLPCRKDCIVTPYSDWATCPTACQDGDVAIKKQTRHRVIIQLPSYGGRDCPDTLYEENECEAPATCHSYRWKTHKWRRCQLVPWYVRQDSPGAYETCGPGLQARAITCRRQDGAQVDVTDCLAKAGPMPPLTQTCQIPCQDDCQFTNWSKFSSCNGDCGAVRTRKRSLLGKSKKKDKCKNAQLYPVIETQFCPCDKYSAQPVGNWSDCILPEGKVEALLGVKVQGDIKECGQGYRYQAMACYDQNGRLVETSRCNSHGYIEEACIIPCPSDCKLSEWSNWSRCSKSCGSGVKVRSKWLREKPYNGGRPCPKLDHVNQAQVYEVVPCHSDCNQYIWVTEPWSICKVTNVDLKQNCGEGVQTRKVRCMQNTVDGPSDVVEDYLCEPEEMPLGARNCKLPCPEDCVMSEWSEWSRCPLPCDGSSYRERSALPIRQAEEGKACHVAIETEPCNLNKNCYHYEYNVTDWSTCQLSEKAVCGNGIKTRMLDCVRSDVKSVDLKYCEELGLEKTWQMNISCVVECPINCQLSEWSPWSKCSKDCGLAGKMLRNRTVIQPSQGDGRPCPSQMEQSKPCPVKPCYRWQYGRWSLCKVEDAQCGVGTRFRNISCVVHDGSREDSGKIVDEEFCGDIDLTVNNDKNIVLEESCTVPCPGDCYLSEWSEWSLCQLTCVNGEDLGFGGIKMRSRAVILQEMENQHLCPEQVVESQPCNDGKCYEYKWLSSPWKGSSRMVWCQRSDGLNVTGGCLEAYHPETERSCNPPCTQQHSYCTETGSCGCEDGYTEVTSSDGTLDQCTLIPVVIMPTLDEKKGDVKTSRAINPTQPSSSQSGYGGRTWFLQPFGSAKSRRNPRDDRTTA

Target ORF information:

RefSeq Version	XM_012965596.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis thrombospondin type 1 domain containing 7A (thsd7a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965596.3

ORF Insert Sequence:

ATGGGGAGAA GCACCGGCTT TTCTGGTACC CTTGCTTCTT CCCTGGGAGT GATTGTTCTT 
CTTCTCGGTG GGATCTGGGC TGCTGAGACG CAGAGTGAAT CTGAAACCAA AACTATATAC 
ATATGGAAGA CTGGTCCCTG GGGCAGATGT ATGGGCGATG AATGTGGACC TGGAGGTATT 
CAAACAAGGG CAGTTTGGTG TGCCCATGTC GAAGGATGGA CGACCCTCCC TACAAACTGT 
AAACAGACAG ACAGGCCCGA AAACCAACAG AACTGTTTTA GAGTTTGCGA CTGGCACAAG 
GAAATGTATG ACTGGCAGTT AGGAAGTTGG AATCAGTGTA AACCAGTTAT ATCCAGATCT 
TCAGAGAAAC CCACAGAATG TACTAAAGGT GAGGAAGGCA TTCAGACTAG AGAAGTAACA 
TGCATCCAGA AAGCAAATGG GGCAGTAGCT GATGATAGTA TATGTGAATA CTTTGAACCT 
AAGCCTCGAC TGGAACAAGG ATGCCTCATT CCCTGCCCTC AAGACTGCAT TGTATCAGAA 
TATTCTTCAT GGTCAGAATG CTCCAGAACA TGTGGCAATG GATTAAAACA CAGAACCCGA 
TATGTTATTA CTTTGGCCCA GTTTGGTGGA TCAGCATGTC CTAACCTTAC AGAATACCAA 
ACATGTCAGT CTGCCCCATG TGAGGGTGAA GAAAATATGT ACAGTCTGAG TGTTGGACCC 
TGGAGTTCTT GCTCAATTCC ACTTTCTAGA CATGTAAGAC AGACTGGTAA ACGAGGCAAA 
AATAGAGAGC GAGCGAAGGA AAGAGAAAAG GCAGTGAAGG ATCCAGAAGC ACGTGAGCTT 
ATTAAAAAGA AAAGAAACCG GAACAGAAAG AATAGACAGG AGAATAGATA TTGGGATATA 
CAGATTGGTT ACCAGACACG ACTAGTAACT TGTATGCACC GGAATGGAAA AACTGCTGCG 
TTAAGTTTTT GCCAACAGGA AAAGTTGCCA ATTACGTTCC AGTCTTGTGT TATTGACAAG 
GAGTGCCAAG TGTCTGAGTG GGCAGAATGG AGCCCTTGCT CCAGGGCTTG TGATATGGAA 
TCTCCTGGTG GATATCGTAC TAGGAAAAGG ACTATCAAAC TATTTCCAAT TGGGAATGGA 
AACGAATGTC CAGAGCTAGA AGAAACAGAA GATTGTACTC CTCCACCAGA CGGGCCACAA 
GTTTGTATTA AATACACCTG GAGAGCTACA GAATGGACTG AATGCCGTGT TGATCCTTTG 
TTAAGCCAGC AGGATAAAAG GAGAGGTAAC CAGACTGCTC TATGCGGCGG AGGAATTCAA 
ACACGGGAGA TTTACTGTGT ACAGGCAAAT GAAAATCTGC TTTCTTACCT CAACAATGTA 
AAAGATAAAG AAGAAAGCCA TTCTTACAGC ACATCAAAGT CTAGTGCGGC ACCATTTCTA 
TCAATCACTT TTGAAATATC AGCATCCAAG CCTGTTGATC CCAAATTGTG CTCGGAATCA 
CCACTAAGTA CAAGCCAGCT CTGCCATGTT CCATGCCCTA TTGAGTGTGA AGTTTCTCCA 
TGGTCAGCAT GGGGACCTTG CACCTTTGAA AACTGTGAAG ACCAACAAAG CAAAAAAGGG 
TTCAAGCTCA GAAAGCGCAA TATTACCAAT GAGCCTACTG GTGGGACTGG CAATTGCCCT 
CACCTTGTAG AAGCCATTCC TTGTGAAGAA CCATCTTGCT ACGACTGGCG ATTGGATAAA 
CTTGAAGAGT GTATTCCTGA CAACGGAAAC AATTGTGGGG CAGGAACTCA GGTGCCTGTT 
GTTACATGCA TTAATAGTGA CGGAGAAGAA GTTGACAGAC AACAGTGCAG AGATGCCATC 
TATCCGATCC CAATTTTGTG TGAAGTTCCC TGCCCAAAGG ATTGTGTTCT CAGCATGTGG 
TCTGAATGGT CGTCCTGCTC ACACACCTGC TCTGGAAAAA CAACAGAAGG ACGGCAGACA 
CGGACGCGGT CTATTCTCGC TTACGCAGGT GATGGAAGAA GTCCGTGCCC AGAAAGCAAC 
GCTCTGCAAG AGATTCGTAG CTGTAATGAG CATCCTTGTA CAGTATATCA CTGGCAGACG 
GGTCCCTGGG GCCAGTGCAC TGAGGATGTC ACTGTTTCAG GTTTCAATTC ATCCAACTGG 
AATGGTGAAG CTACTTGTGC TCTAGGGATG CAAACAAGAA AAATCATTTG TGTTCGAGTC 
AACGTGGGAC AGGTTGGACC CAAAAAATGT CCTGAAAGCC TTCGTCCAGA AACCATGAGG 
CCATGTCTGC TTCCATGTCG AAAGGATTGC ATAGTTACAC CATATAGTGA CTGGGCCACT 
TGCCCCACCG CCTGTCAAGA CGGTGATGTT GCCATAAAAA AACAGACTCG TCATCGTGTC 
ATTATCCAAC TCCCATCTTA TGGAGGCCGT GACTGCCCCG ATACTTTATA CGAAGAAAAT 
GAATGCGAAG CTCCAGCAAC CTGTCACAGC TACCGCTGGA AAACACATAA ATGGAGAAGA 
TGCCAGTTGG TGCCGTGGTA CGTCCGACAA GACAGCCCTG GAGCTTATGA AACGTGTGGG 
CCTGGATTAC AAGCCAGAGC AATTACTTGC CGCAGACAAG ATGGTGCACA GGTTGATGTC 
ACTGATTGTC TTGCCAAAGC TGGTCCAATG CCTCCTTTAA CCCAGACCTG CCAGATACCT 
TGCCAAGATG ACTGTCAATT TACTAACTGG TCGAAATTTT CTTCATGTAA TGGTGACTGT 
GGTGCAGTAA GGACAAGAAA GCGATCACTT CTTGGGAAAA GTAAGAAAAA AGACAAGTGC 
AAGAACGCCC AGCTCTACCC AGTGATTGAG ACTCAGTTCT GCCCATGTGA TAAATACAGT 
GCTCAGCCTG TGGGAAACTG GTCAGACTGC ATCTTGCCAG AAGGAAAAGT GGAGGCCTTA 
CTGGGGGTAA AAGTACAAGG AGACATCAAG GAGTGTGGGC AGGGCTATCG TTACCAAGCC 
ATGGCATGTT ATGATCAAAA CGGCAGACTT GTGGAGACTT CAAGGTGTAA CAGCCATGGT 
TATATTGAGG AAGCATGTAT CATACCCTGT CCATCTGATT GTAAGCTTAG CGAATGGTCC 
AACTGGTCAC GGTGTAGCAA GTCCTGTGGG AGTGGAGTCA AGGTTCGGTC GAAATGGCTC 
CGTGAAAAAC CTTATAATGG TGGAAGACCT TGTCCTAAAC TGGATCACGT AAACCAGGCT 
CAGGTATATG AAGTGGTTCC ATGTCACAGT GATTGCAATC AGTATATATG GGTCACAGAG 
CCTTGGAGTA TTTGCAAAGT GACTAATGTT GATCTAAAGC AGAACTGCGG CGAAGGTGTT 
CAGACAAGAA AAGTAAGATG CATGCAGAAC ACAGTAGATG GCCCTTCGGA TGTTGTGGAA 
GACTATCTAT GTGAACCCGA GGAAATGCCA TTGGGCGCCA GAAACTGCAA GCTGCCCTGT 
CCTGAAGACT GTGTGATGTC TGAATGGAGT GAATGGAGCA GGTGTCCCTT GCCCTGTGAC 
GGAAGCAGCT ACCGAGAGAG GTCAGCCCTA CCTATCCGAC AAGCCGAGGA AGGCAAAGCC 
TGCCATGTTG CAATTGAAAC GGAGCCCTGC AACCTGAATA AAAACTGTTA TCACTATGAA 
TACAATGTCA CGGACTGGAG CACTTGCCAG CTAAGTGAGA AGGCAGTTTG TGGAAACGGG 
ATAAAAACAA GAATGCTGGA CTGCGTCCGC AGCGATGTCA AATCAGTTGA CTTGAAATAC 
TGTGAAGAGC TTGGCTTGGA AAAGACATGG CAGATGAATA TTTCTTGTGT GGTGGAATGT 
CCTATCAACT GTCAACTCTC CGAATGGTCA CCATGGTCCA AATGTTCCAA AGACTGTGGC 
CTAGCAGGTA AAATGTTACG GAATAGAACA GTGATTCAGC CGTCCCAGGG TGATGGAAGA 
CCTTGCCCTT CACAGATGGA GCAATCCAAA CCCTGCCCAG TAAAGCCATG TTACCGGTGG 
CAGTATGGAC GGTGGTCTTT GTGCAAAGTT GAGGATGCTC AGTGTGGTGT AGGAACAAGG 
TTCAGAAACA TTTCTTGTGT AGTGCATGAT GGATCCAGGG AAGATTCTGG TAAAATAGTA 
GACGAGGAGT TTTGCGGAGA CATTGACCTT ACTGTAAATA ATGACAAGAA CATTGTGCTA 
GAGGAATCAT GCACCGTTCC ATGCCCAGGT GATTGTTACT TGAGCGAATG GTCTGAGTGG 
AGTTTGTGTC AGCTGACTTG TGTTAATGGA GAAGATCTTG GCTTTGGAGG AATAAAGATG 
CGATCGCGTG CAGTAATTCT TCAAGAAATG GAAAATCAAC ATCTGTGTCC TGAACAAGTT 
GTGGAATCAC AACCATGTAA TGATGGAAAG TGTTATGAAT ATAAATGGCT GTCAAGTCCT 
TGGAAAGGAT CTTCTCGAAT GGTCTGGTGT CAGAGGTCAG ATGGCTTAAA TGTTACAGGA 
GGCTGTTTAG AGGCTTATCA TCCAGAAACT GAAAGATCGT GTAATCCACC TTGCACGCAG 
CAACACTCAT ATTGCACCGA GACAGGGTCA TGTGGCTGTG AAGATGGATA TACTGAAGTG 
ACATCGTCTG ATGGAACACT AGATCAGTGT ACTCTGATCC CAGTGGTGAT AATGCCAACA 
TTAGATGAAA AAAAGGGAGA CGTTAAAACG AGTCGTGCCA TCAACCCAAC CCAGCCATCC 
AGTAGTCAGT CGGGTTATGG AGGAAGAACA TGGTTTTTAC AACCCTTCGG TTCAGCAAAA 
AGCCGAAGAA ACCCCCGAGA CGACAGAACA ACCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

thsd7a ( XM_012965595.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821049.1 Xenopus tropicalis thrombospondin type 1 domain containing 7A (thsd7a), transcript variant X1, mRNA.

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CloneID	OXa11988
Clone ID Related Accession (Same CDS sequence)	XM_012965595.2
Accession Version	XM_012965595.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	thrombospondin type-1 domain-containing protein 7A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965595.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGAA GCACCGGCTT TTCTGGTACC CTTGCTTCTT CCCTGGGAGT GATTGTTCTT 
CTTCTCGGTG GGATCTGGGC TGCTGAGACG CAGAGTGAAT CTGAAACCAA AACTATATAC 
ATATGGAAGA CTGGTCCCTG GGGCAGATGT ATGGGCGATG AATGTGGACC TGGAGGTATT 
CAAACAAGGG CAGTTTGGTG TGCCCATGTC GAAGGATGGA CGACCCTCCC TACAAACTGT 
AAACAGACAG ACAGGCCCGA AAACCAACAG AACTGTTTTA GAGTTTGCGA CTGGCACAAG 
GAAATGTATG ACTGGCAGTT AGGAAGTTGG AATCAGTGTA AACCAGTTAT ATCCAGATCT 
TCAGAGAAAC CCACAGAATG TACTAAAGGT GAGGAAGGCA TTCAGACTAG AGAAGTAACA 
TGCATCCAGA AAGCAAATGG GGCAGTAGCT GATGATAGTA TATGTGAATA CTTTGAACCT 
AAGCCTCGAC TGGAACAAGG ATGCCTCATT CCCTGCCCTC AAGACTGCAT TGTATCAGAA 
TATTCTTCAT GGTCAGAATG CTCCAGAACA TGTGGCAATG GATTAAAACA CAGAACCCGA 
TATGTTATTA CTTTGGCCCA GTTTGGTGGA TCAGCATGTC CTAACCTTAC AGAATACCAA 
ACATGTCAGT CTGCCCCATG TGAGGGTGAA GAAAATATGT ACAGTCTGAG TGTTGGACCC 
TGGAGTTCTT GCTCAATTCC ACTTTCTAGA CATGTAAGAC AGACTGGTAA ACGAGGCAAA 
AATAGAGAGC GAGCGAAGGA AAGAGAAAAG GCAGTGAAGG ATCCAGAAGC ACGTGAGCTT 
ATTAAAAAGA AAAGAAACCG GAACAGAAAG AATAGACAGG AGAATAGATA TTGGGATATA 
CAGATTGGTT ACCAGACACG ACTAGTAACT TGTATGCACC GGAATGGAAA AACTGCTGCG 
TTAAGTTTTT GTCAACAGGA AAAGTTGCCA ATTACGTTCC AGTCTTGTGT TATTGACAAG 
GAGTGCCAAG TGTCTGAGTG GGCAGAATGG AGCCCTTGCT CCAGGGCTTG TGATATGGAA 
TCTCCTGGTG GATATCGTAC TAGGAAAAGG ACTATCAAAC TATTTCCAAT TGGGAATGGA 
AACGAATGTC CAGAGCTAGA AGAAACAGAA GATTGTACTC CTCCACCAGA CGGGCCACAA 
GTTTGTATTA AATACACCTG GAGAGCTACA GAATGGACTG AATGCCGTGT TGATCCTTTG 
TTAAGCCAGC AGGATAAAAG GAGAGGTAAC CAGACTGCTC TATGCGGCGG AGGAATTCAA 
ACACGGGAGA TTTACTGTGT ACAGGCAAAT GAAAATCTGC TTTCTTACCT CAACAATGTA 
AAAGATAAAG AAGAAAGCCA TTCTTACAGC ACATCAAAGT CTAGTGCGGC ACCATTTCTA 
TCAATCACTT TTGAAATATC AGCATCCAAG CCTGTTGATC CCAAATTGTG CTCGGAATCA 
CCGCTAAGTA CAAGCCAGCT CTGCCATGTT CCATGCCCTA TTGAGTGTGA AGTTTCTCCA 
TGGTCAGCAT GGGGACCTTG CACCTTTGAA AACTGTGAAG ACCAACAAAG CAAAAAAGGG 
TTCAAGCTCA GAAAGCGCAA TATTACCAAT GAGCCTACTG GTGGGACTGG CAATTGCCCT 
CACCTTGTAG AAGCCATTCC TTGTGAAGAA CCATCTTGCT ACGACTGGCG ATTGGATAAA 
CTTGAAGAGT GTATTCCTGA CAACGGAAAC AATTGTGGGG CAGGAACTCA GGTGCCTGTT 
GTTACATGCA TTAATAGTGA CGGAGAAGAA GTTGACAGAC AACAGTGCAG AGATGCCATC 
TATCCGATCC CAATTTTGTG TGAAGTTCCC TGCCCAAAGG ATTGTGTTCT CAGCATGTGG 
TCTGAATGGT CGTCCTGCTC ACACACCTGC TCTGGAAAAA CAACAGAAGG ACGGCAGACA 
CGGACGCGGT CTATTCTCGC TTACGCAGGT GATGGAAGAA GTCCGTGCCC AGAAAGCAAC 
GCTCTGCAAG AGATTCGTAG CTGTAATGAG CATCCTTGTA CAGTATATCA CTGGCAGACG 
GGTCCCTGGG GCCAGTGCAC TGAGGATGTC ACTGTTTCAG GTTTCAATTC ATCCAACTGG 
AATGGTGAAG CTACTTGTGC TCTAGGGATG CAAACAAGAA AAATCATTTG TGTTCGAGTC 
AACGTGGGAC AGGTTGGACC CAAAAAATGT CCTGAAAGCC TTCGTCCAGA AACCATGAGG 
CCATGTCTGC TTCCATGTCG AAAGGATTGC ATAGTTACAC CATATAGTGA CTGGGCCACT 
TGCCCCACCG CCTGTCAAGA CGGTGATGTT GCCATAAAAA AACAGACTCG TCATCGTGTC 
ATTATCCAAC TCCCATCTTA TGGAGGCCGT GACTGCCCCG ATACTTTATA CGAAGAAAAT 
GAATGCGAAG CTCCAGCAAC CTGTCACAGC TACCGCTGGA AAACACATAA ATGGAGAAGA 
TGCCAGTTGG TGCCGTGGTA CGTCCGACAA GACAGCCCTG GAGCTTATGA AACGTGTGGG 
CCTGGATTAC AAGCCAGAGC AATTACTTGC CGCAGACAAG ATGGTGCACA GGTTGATGTC 
ACTGATTGTC TTGCCAAAGC TGGTCCAATG CCTCCTTTAA CCCAGACCTG CCAGATACCT 
TGCCAAGATG ACTGTCAATT TACTAACTGG TCGAAATTTT CTTCATGTAA TGGTGACTGT 
GGTGCAGTAA GGACAAGAAA GCGATCACTT CTTGGGAAAA GTAAGAAAAA AGACAAGTGC 
AAGAACGCCC AGCTCTACCC AGTGATTGAG ACTCAGTTCT GCCCATGTGA TAAATACAGT 
GCTCAGCCTG TGGGAAACTG GTCAGACTGC ATCTTGCCAG AAGGAAAAGT GGAGGCCTTA 
CTGGGGGTAA AAGTACAAGG AGACATCAAG GAGTGTGGGC AGGGCTATCG TTACCAAGCC 
ATGGCATGTT ATGATCAAAA CGGCAGACTT GTGGAGACTT CAAGGTGTAA CAGCCATGGT 
TATATTGAGG AAGCATGTAT CATACCCTGT CCATCTGATT GTAAGCTTAG CGAATGGTCC 
AACTGGTCAC GGTGTAGCAA GTCCTGTGGG AGTGGAGTCA AGGTTCGGTC GAAATGGCTC 
CGTGAAAAAC CTTATAATGG TGGAAGACCT TGTCCTAAAC TGGATCACGT AAACCAGGCT 
CAGGTATATG AAGTGGTTCC ATGTCACAGT GATTGCAATC AGTATATATG GGTCACAGAG 
CCTTGGAGTA TTTGCAAAGT TACTAATGTT GATCTAAAGC AAAACTGCGG CGAAGGTGTT 
CAGACAAGAA AAGTAAGATG CATGCAGAAC ACAGTAGATG GCCCTTCGGA TGTTGTGGAA 
GACTATCTAT GTGAACCTGA GGAAATGCCA TTGGGCGCCA GGAACTGCAA GCTGCCCTGT 
CCTGAAGACT GTGTGATGTC TGAATGGAGT GAATGGAGCA GGTGTCCCTT GCCCTGTGAC 
GGAAGCAGCT ACCGAGAGAG GTCAGCCCTA CCTATCCGAC AAGCCGAGGA AGGCAAAGCC 
TGCCATGTTG CAATTGAAAC GGAGCCCTGC AACCTGAATA AAAACTGTTA TCACTATGAA 
TACAATGTCA CGGACTGGAG CACTTGCCAG CTAAGTGAGA AGGCAGTTTG TGGAAACGGG 
ATAAAAACAA GAATGCTGGA CTGCGTCCGC AGCGATGTCA AATCAGTTGA CTTGAAATAC 
TGTGAAGAGC TTGGCTTGGA AAAGACATGG CAGATGAATA TTTCTTGTGT GGTGGAATGT 
CCTATCAACT GTCAACTCTC CGAATGGTCA CCATGGTCCA AATGTTCCAA AGACTGTGGC 
CTAGCAGGTA AAATGTTACG GAATAGAACA GTGATTCAGC CGTCCCAGGG TGATGGAAGA 
CCTTGCCCTT CACAGATGGA GCAATCCAAA CCCTGCCCAG TAAAGCCATG TTACCGGTGG 
CAGTATGGAC GGTGGTCTTT GTGCAAAGTT GAGGATGCTC AGTGTGGTGT AGGAACAAGG 
TTCAGAAACA TTTCTTGTGT AGTGCATGAT GGATCCAGGG AAGATTCTGG TAAAATAGTA 
GACGAGGAGT TTTGCGGAGA CATTGACCTT ACTGTAAATA ATGACAAGAA CATTGTGCTA 
GAGGAATCAT GCACCGTTCC ATGCCCAGGT GATTGTTACT TGAGCGAATG GTCTGAGTGG 
AGTTTGTGTC AGCTGACTTG TGTTAATGGA GAAGATCTTG GCTTTGGAGG AATAAAGATG 
CGATCGCGTG CAGTAATTCT TCAAGAAATG GAAAATCAAC ATCTGTGTCC TGAACAAGTT 
GTGGAATCAC AACCATGTAA TGATGGAAAG TGTTATGAAT ATAAATGGCT GTCAAGTCCT 
TGGAAAGGAT CTTCTCGAAT GGTCTGGTGT CAGAGGTCAG ATGGCTTAAA TGTTACAGGA 
GGCTGTTTAG AGGCTTATCA TCCAGAAACT GAAAGATCGT GTAATCCACC TTGCACGCAG 
CAACACTCAT ATTGCACCGA GACAGGGTCA TGTGGCTGTG AAGATGGATA TACTGAAGTG 
ACATCGTCTG ATGGAACACT AGATCAGTGT ACTCTGATCC CAGTGGTGAT AATGCCAACA 
TTAGATGAAA AAAAGGGAGA CGTTAAAACG AGTCGTGCCA TCAACCCAAC CCAGCCATCC 
AGTAGTCAGT CGGGTTATGG AGGAAGAACA TGGTTTTTAC AACCCTTCGG TTCAGACGGT 
AGACTGAAGA CCTGGGTGTA TGGGGTAGCA GCTGGAGCCT TTGTGTTACT CATCTTTGTT 
GTTTCTATGA TCTATTTGGC CTGCAAAAAG CCGAAGAAAC CCCCGAGACG ACAGAACAAC 
CGCCTGAAAC CCTTAACTCT GGCCTACGAT GGAGATGCAG ACATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821049.1
CDS	330..5297
Translation	MGRSTGFSGTLASSLGVIVLLLGGIWAAETQSESETKTIYIWKTGPWGRCMGDECGPGGIQTRAVWCAHVEGWTTLPTNCKQTDRPENQQNCFRVCDWHKEMYDWQLGSWNQCKPVISRSSEKPTECTKGEEGIQTREVTCIQKANGAVADDSICEYFEPKPRLEQGCLIPCPQDCIVSEYSSWSECSRTCGNGLKHRTRYVITLAQFGGSACPNLTEYQTCQSAPCEGEENMYSLSVGPWSSCSIPLSRHVRQTGKRGKNRERAKEREKAVKDPEARELIKKKRNRNRKNRQENRYWDIQIGYQTRLVTCMHRNGKTAALSFCQQEKLPITFQSCVIDKECQVSEWAEWSPCSRACDMESPGGYRTRKRTIKLFPIGNGNECPELEETEDCTPPPDGPQVCIKYTWRATEWTECRVDPLLSQQDKRRGNQTALCGGGIQTREIYCVQANENLLSYLNNVKDKEESHSYSTSKSSAAPFLSITFEISASKPVDPKLCSESPLSTSQLCHVPCPIECEVSPWSAWGPCTFENCEDQQSKKGFKLRKRNITNEPTGGTGNCPHLVEAIPCEEPSCYDWRLDKLEECIPDNGNNCGAGTQVPVVTCINSDGEEVDRQQCRDAIYPIPILCEVPCPKDCVLSMWSEWSSCSHTCSGKTTEGRQTRTRSILAYAGDGRSPCPESNALQEIRSCNEHPCTVYHWQTGPWGQCTEDVTVSGFNSSNWNGEATCALGMQTRKIICVRVNVGQVGPKKCPESLRPETMRPCLLPCRKDCIVTPYSDWATCPTACQDGDVAIKKQTRHRVIIQLPSYGGRDCPDTLYEENECEAPATCHSYRWKTHKWRRCQLVPWYVRQDSPGAYETCGPGLQARAITCRRQDGAQVDVTDCLAKAGPMPPLTQTCQIPCQDDCQFTNWSKFSSCNGDCGAVRTRKRSLLGKSKKKDKCKNAQLYPVIETQFCPCDKYSAQPVGNWSDCILPEGKVEALLGVKVQGDIKECGQGYRYQAMACYDQNGRLVETSRCNSHGYIEEACIIPCPSDCKLSEWSNWSRCSKSCGSGVKVRSKWLREKPYNGGRPCPKLDHVNQAQVYEVVPCHSDCNQYIWVTEPWSICKVTNVDLKQNCGEGVQTRKVRCMQNTVDGPSDVVEDYLCEPEEMPLGARNCKLPCPEDCVMSEWSEWSRCPLPCDGSSYRERSALPIRQAEEGKACHVAIETEPCNLNKNCYHYEYNVTDWSTCQLSEKAVCGNGIKTRMLDCVRSDVKSVDLKYCEELGLEKTWQMNISCVVECPINCQLSEWSPWSKCSKDCGLAGKMLRNRTVIQPSQGDGRPCPSQMEQSKPCPVKPCYRWQYGRWSLCKVEDAQCGVGTRFRNISCVVHDGSREDSGKIVDEEFCGDIDLTVNNDKNIVLEESCTVPCPGDCYLSEWSEWSLCQLTCVNGEDLGFGGIKMRSRAVILQEMENQHLCPEQVVESQPCNDGKCYEYKWLSSPWKGSSRMVWCQRSDGLNVTGGCLEAYHPETERSCNPPCTQQHSYCTETGSCGCEDGYTEVTSSDGTLDQCTLIPVVIMPTLDEKKGDVKTSRAINPTQPSSSQSGYGGRTWFLQPFGSDGRLKTWVYGVAAGAFVLLIFVVSMIYLACKKPKKPPRRQNNRLKPLTLAYDGDADM

Target ORF information:

RefSeq Version	XM_012965595.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis thrombospondin type 1 domain containing 7A (thsd7a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965595.2

ORF Insert Sequence:

ATGGGGAGAA GCACCGGCTT TTCTGGTACC CTTGCTTCTT CCCTGGGAGT GATTGTTCTT 
CTTCTCGGTG GGATCTGGGC TGCTGAGACG CAGAGTGAAT CTGAAACCAA AACTATATAC 
ATATGGAAGA CTGGTCCCTG GGGCAGATGT ATGGGCGATG AATGTGGACC TGGAGGTATT 
CAAACAAGGG CAGTTTGGTG TGCCCATGTC GAAGGATGGA CGACCCTCCC TACAAACTGT 
AAACAGACAG ACAGGCCCGA AAACCAACAG AACTGTTTTA GAGTTTGCGA CTGGCACAAG 
GAAATGTATG ACTGGCAGTT AGGAAGTTGG AATCAGTGTA AACCAGTTAT ATCCAGATCT 
TCAGAGAAAC CCACAGAATG TACTAAAGGT GAGGAAGGCA TTCAGACTAG AGAAGTAACA 
TGCATCCAGA AAGCAAATGG GGCAGTAGCT GATGATAGTA TATGTGAATA CTTTGAACCT 
AAGCCTCGAC TGGAACAAGG ATGCCTCATT CCCTGCCCTC AAGACTGCAT TGTATCAGAA 
TATTCTTCAT GGTCAGAATG CTCCAGAACA TGTGGCAATG GATTAAAACA CAGAACCCGA 
TATGTTATTA CTTTGGCCCA GTTTGGTGGA TCAGCATGTC CTAACCTTAC AGAATACCAA 
ACATGTCAGT CTGCCCCATG TGAGGGTGAA GAAAATATGT ACAGTCTGAG TGTTGGACCC 
TGGAGTTCTT GCTCAATTCC ACTTTCTAGA CATGTAAGAC AGACTGGTAA ACGAGGCAAA 
AATAGAGAGC GAGCGAAGGA AAGAGAAAAG GCAGTGAAGG ATCCAGAAGC ACGTGAGCTT 
ATTAAAAAGA AAAGAAACCG GAACAGAAAG AATAGACAGG AGAATAGATA TTGGGATATA 
CAGATTGGTT ACCAGACACG ACTAGTAACT TGTATGCACC GGAATGGAAA AACTGCTGCG 
TTAAGTTTTT GTCAACAGGA AAAGTTGCCA ATTACGTTCC AGTCTTGTGT TATTGACAAG 
GAGTGCCAAG TGTCTGAGTG GGCAGAATGG AGCCCTTGCT CCAGGGCTTG TGATATGGAA 
TCTCCTGGTG GATATCGTAC TAGGAAAAGG ACTATCAAAC TATTTCCAAT TGGGAATGGA 
AACGAATGTC CAGAGCTAGA AGAAACAGAA GATTGTACTC CTCCACCAGA CGGGCCACAA 
GTTTGTATTA AATACACCTG GAGAGCTACA GAATGGACTG AATGCCGTGT TGATCCTTTG 
TTAAGCCAGC AGGATAAAAG GAGAGGTAAC CAGACTGCTC TATGCGGCGG AGGAATTCAA 
ACACGGGAGA TTTACTGTGT ACAGGCAAAT GAAAATCTGC TTTCTTACCT CAACAATGTA 
AAAGATAAAG AAGAAAGCCA TTCTTACAGC ACATCAAAGT CTAGTGCGGC ACCATTTCTA 
TCAATCACTT TTGAAATATC AGCATCCAAG CCTGTTGATC CCAAATTGTG CTCGGAATCA 
CCGCTAAGTA CAAGCCAGCT CTGCCATGTT CCATGCCCTA TTGAGTGTGA AGTTTCTCCA 
TGGTCAGCAT GGGGACCTTG CACCTTTGAA AACTGTGAAG ACCAACAAAG CAAAAAAGGG 
TTCAAGCTCA GAAAGCGCAA TATTACCAAT GAGCCTACTG GTGGGACTGG CAATTGCCCT 
CACCTTGTAG AAGCCATTCC TTGTGAAGAA CCATCTTGCT ACGACTGGCG ATTGGATAAA 
CTTGAAGAGT GTATTCCTGA CAACGGAAAC AATTGTGGGG CAGGAACTCA GGTGCCTGTT 
GTTACATGCA TTAATAGTGA CGGAGAAGAA GTTGACAGAC AACAGTGCAG AGATGCCATC 
TATCCGATCC CAATTTTGTG TGAAGTTCCC TGCCCAAAGG ATTGTGTTCT CAGCATGTGG 
TCTGAATGGT CGTCCTGCTC ACACACCTGC TCTGGAAAAA CAACAGAAGG ACGGCAGACA 
CGGACGCGGT CTATTCTCGC TTACGCAGGT GATGGAAGAA GTCCGTGCCC AGAAAGCAAC 
GCTCTGCAAG AGATTCGTAG CTGTAATGAG CATCCTTGTA CAGTATATCA CTGGCAGACG 
GGTCCCTGGG GCCAGTGCAC TGAGGATGTC ACTGTTTCAG GTTTCAATTC ATCCAACTGG 
AATGGTGAAG CTACTTGTGC TCTAGGGATG CAAACAAGAA AAATCATTTG TGTTCGAGTC 
AACGTGGGAC AGGTTGGACC CAAAAAATGT CCTGAAAGCC TTCGTCCAGA AACCATGAGG 
CCATGTCTGC TTCCATGTCG AAAGGATTGC ATAGTTACAC CATATAGTGA CTGGGCCACT 
TGCCCCACCG CCTGTCAAGA CGGTGATGTT GCCATAAAAA AACAGACTCG TCATCGTGTC 
ATTATCCAAC TCCCATCTTA TGGAGGCCGT GACTGCCCCG ATACTTTATA CGAAGAAAAT 
GAATGCGAAG CTCCAGCAAC CTGTCACAGC TACCGCTGGA AAACACATAA ATGGAGAAGA 
TGCCAGTTGG TGCCGTGGTA CGTCCGACAA GACAGCCCTG GAGCTTATGA AACGTGTGGG 
CCTGGATTAC AAGCCAGAGC AATTACTTGC CGCAGACAAG ATGGTGCACA GGTTGATGTC 
ACTGATTGTC TTGCCAAAGC TGGTCCAATG CCTCCTTTAA CCCAGACCTG CCAGATACCT 
TGCCAAGATG ACTGTCAATT TACTAACTGG TCGAAATTTT CTTCATGTAA TGGTGACTGT 
GGTGCAGTAA GGACAAGAAA GCGATCACTT CTTGGGAAAA GTAAGAAAAA AGACAAGTGC 
AAGAACGCCC AGCTCTACCC AGTGATTGAG ACTCAGTTCT GCCCATGTGA TAAATACAGT 
GCTCAGCCTG TGGGAAACTG GTCAGACTGC ATCTTGCCAG AAGGAAAAGT GGAGGCCTTA 
CTGGGGGTAA AAGTACAAGG AGACATCAAG GAGTGTGGGC AGGGCTATCG TTACCAAGCC 
ATGGCATGTT ATGATCAAAA CGGCAGACTT GTGGAGACTT CAAGGTGTAA CAGCCATGGT 
TATATTGAGG AAGCATGTAT CATACCCTGT CCATCTGATT GTAAGCTTAG CGAATGGTCC 
AACTGGTCAC GGTGTAGCAA GTCCTGTGGG AGTGGAGTCA AGGTTCGGTC GAAATGGCTC 
CGTGAAAAAC CTTATAATGG TGGAAGACCT TGTCCTAAAC TGGATCACGT AAACCAGGCT 
CAGGTATATG AAGTGGTTCC ATGTCACAGT GATTGCAATC AGTATATATG GGTCACAGAG 
CCTTGGAGTA TTTGCAAAGT TACTAATGTT GATCTAAAGC AAAACTGCGG CGAAGGTGTT 
CAGACAAGAA AAGTAAGATG CATGCAGAAC ACAGTAGATG GCCCTTCGGA TGTTGTGGAA 
GACTATCTAT GTGAACCTGA GGAAATGCCA TTGGGCGCCA GGAACTGCAA GCTGCCCTGT 
CCTGAAGACT GTGTGATGTC TGAATGGAGT GAATGGAGCA GGTGTCCCTT GCCCTGTGAC 
GGAAGCAGCT ACCGAGAGAG GTCAGCCCTA CCTATCCGAC AAGCCGAGGA AGGCAAAGCC 
TGCCATGTTG CAATTGAAAC GGAGCCCTGC AACCTGAATA AAAACTGTTA TCACTATGAA 
TACAATGTCA CGGACTGGAG CACTTGCCAG CTAAGTGAGA AGGCAGTTTG TGGAAACGGG 
ATAAAAACAA GAATGCTGGA CTGCGTCCGC AGCGATGTCA AATCAGTTGA CTTGAAATAC 
TGTGAAGAGC TTGGCTTGGA AAAGACATGG CAGATGAATA TTTCTTGTGT GGTGGAATGT 
CCTATCAACT GTCAACTCTC CGAATGGTCA CCATGGTCCA AATGTTCCAA AGACTGTGGC 
CTAGCAGGTA AAATGTTACG GAATAGAACA GTGATTCAGC CGTCCCAGGG TGATGGAAGA 
CCTTGCCCTT CACAGATGGA GCAATCCAAA CCCTGCCCAG TAAAGCCATG TTACCGGTGG 
CAGTATGGAC GGTGGTCTTT GTGCAAAGTT GAGGATGCTC AGTGTGGTGT AGGAACAAGG 
TTCAGAAACA TTTCTTGTGT AGTGCATGAT GGATCCAGGG AAGATTCTGG TAAAATAGTA 
GACGAGGAGT TTTGCGGAGA CATTGACCTT ACTGTAAATA ATGACAAGAA CATTGTGCTA 
GAGGAATCAT GCACCGTTCC ATGCCCAGGT GATTGTTACT TGAGCGAATG GTCTGAGTGG 
AGTTTGTGTC AGCTGACTTG TGTTAATGGA GAAGATCTTG GCTTTGGAGG AATAAAGATG 
CGATCGCGTG CAGTAATTCT TCAAGAAATG GAAAATCAAC ATCTGTGTCC TGAACAAGTT 
GTGGAATCAC AACCATGTAA TGATGGAAAG TGTTATGAAT ATAAATGGCT GTCAAGTCCT 
TGGAAAGGAT CTTCTCGAAT GGTCTGGTGT CAGAGGTCAG ATGGCTTAAA TGTTACAGGA 
GGCTGTTTAG AGGCTTATCA TCCAGAAACT GAAAGATCGT GTAATCCACC TTGCACGCAG 
CAACACTCAT ATTGCACCGA GACAGGGTCA TGTGGCTGTG AAGATGGATA TACTGAAGTG 
ACATCGTCTG ATGGAACACT AGATCAGTGT ACTCTGATCC CAGTGGTGAT AATGCCAACA 
TTAGATGAAA AAAAGGGAGA CGTTAAAACG AGTCGTGCCA TCAACCCAAC CCAGCCATCC 
AGTAGTCAGT CGGGTTATGG AGGAAGAACA TGGTTTTTAC AACCCTTCGG TTCAGACGGT 
AGACTGAAGA CCTGGGTGTA TGGGGTAGCA GCTGGAGCCT TTGTGTTACT CATCTTTGTT 
GTTTCTATGA TCTATTTGGC CTGCAAAAAG CCGAAGAAAC CCCCGAGACG ACAGAACAAC 
CGCCTGAAAC CCTTAACTCT GGCCTACGAT GGAGATGCAG ACATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

thsd7a ( XM_012965596.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821050.1 Xenopus tropicalis thrombospondin type 1 domain containing 7A (thsd7a), transcript variant X2, mRNA.

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CloneID	OXa36335
Clone ID Related Accession (Same CDS sequence)	XM_012965596.2
Accession Version	XM_012965596.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	thrombospondin type-1 domain-containing protein 7A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965596.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGAA GCACCGGCTT TTCTGGTACC CTTGCTTCTT CCCTGGGAGT GATTGTTCTT 
CTTCTCGGTG GGATCTGGGC TGCTGAGACG CAGAGTGAAT CTGAAACCAA AACTATATAC 
ATATGGAAGA CTGGTCCCTG GGGCAGATGT ATGGGCGATG AATGTGGACC TGGAGGTATT 
CAAACAAGGG CAGTTTGGTG TGCCCATGTC GAAGGATGGA CGACCCTCCC TACAAACTGT 
AAACAGACAG ACAGGCCCGA AAACCAACAG AACTGTTTTA GAGTTTGCGA CTGGCACAAG 
GAAATGTATG ACTGGCAGTT AGGAAGTTGG AATCAGTGTA AACCAGTTAT ATCCAGATCT 
TCAGAGAAAC CCACAGAATG TACTAAAGGT GAGGAAGGCA TTCAGACTAG AGAAGTAACA 
TGCATCCAGA AAGCAAATGG GGCAGTAGCT GATGATAGTA TATGTGAATA CTTTGAACCT 
AAGCCTCGAC TGGAACAAGG ATGCCTCATT CCCTGCCCTC AAGACTGCAT TGTATCAGAA 
TATTCTTCAT GGTCAGAATG CTCCAGAACA TGTGGCAATG GATTAAAACA CAGAACCCGA 
TATGTTATTA CTTTGGCCCA GTTTGGTGGA TCAGCATGTC CTAACCTTAC AGAATACCAA 
ACATGTCAGT CTGCCCCATG TGAGGGTGAA GAAAATATGT ACAGTCTGAG TGTTGGACCC 
TGGAGTTCTT GCTCAATTCC ACTTTCTAGA CATGTAAGAC AGACTGGTAA ACGAGGCAAA 
AATAGAGAGC GAGCGAAGGA AAGAGAAAAG GCAGTGAAGG ATCCAGAAGC ACGTGAGCTT 
ATTAAAAAGA AAAGAAACCG GAACAGAAAG AATAGACAGG AGAATAGATA TTGGGATATA 
CAGATTGGTT ACCAGACACG ACTAGTAACT TGTATGCACC GGAATGGAAA AACTGCTGCG 
TTAAGTTTTT GTCAACAGGA AAAGTTGCCA ATTACGTTCC AGTCTTGTGT TATTGACAAG 
GAGTGCCAAG TGTCTGAGTG GGCAGAATGG AGCCCTTGCT CCAGGGCTTG TGATATGGAA 
TCTCCTGGTG GATATCGTAC TAGGAAAAGG ACTATCAAAC TATTTCCAAT TGGGAATGGA 
AACGAATGTC CAGAGCTAGA AGAAACAGAA GATTGTACTC CTCCACCAGA CGGGCCACAA 
GTTTGTATTA AATACACCTG GAGAGCTACA GAATGGACTG AATGCCGTGT TGATCCTTTG 
TTAAGCCAGC AGGATAAAAG GAGAGGTAAC CAGACTGCTC TATGCGGCGG AGGAATTCAA 
ACACGGGAGA TTTACTGTGT ACAGGCAAAT GAAAATCTGC TTTCTTACCT CAACAATGTA 
AAAGATAAAG AAGAAAGCCA TTCTTACAGC ACATCAAAGT CTAGTGCGGC ACCATTTCTA 
TCAATCACTT TTGAAATATC AGCATCCAAG CCTGTTGATC CCAAATTGTG CTCGGAATCA 
CCGCTAAGTA CAAGCCAGCT CTGCCATGTT CCATGCCCTA TTGAGTGTGA AGTTTCTCCA 
TGGTCAGCAT GGGGACCTTG CACCTTTGAA AACTGTGAAG ACCAACAAAG CAAAAAAGGG 
TTCAAGCTCA GAAAGCGCAA TATTACCAAT GAGCCTACTG GTGGGACTGG CAATTGCCCT 
CACCTTGTAG AAGCCATTCC TTGTGAAGAA CCATCTTGCT ACGACTGGCG ATTGGATAAA 
CTTGAAGAGT GTATTCCTGA CAACGGAAAC AATTGTGGGG CAGGAACTCA GGTGCCTGTT 
GTTACATGCA TTAATAGTGA CGGAGAAGAA GTTGACAGAC AACAGTGCAG AGATGCCATC 
TATCCGATCC CAATTTTGTG TGAAGTTCCC TGCCCAAAGG ATTGTGTTCT CAGCATGTGG 
TCTGAATGGT CGTCCTGCTC ACACACCTGC TCTGGAAAAA CAACAGAAGG ACGGCAGACA 
CGGACGCGGT CTATTCTCGC TTACGCAGGT GATGGAAGAA GTCCGTGCCC AGAAAGCAAC 
GCTCTGCAAG AGATTCGTAG CTGTAATGAG CATCCTTGTA CAGTATATCA CTGGCAGACG 
GGTCCCTGGG GCCAGTGCAC TGAGGATGTC ACTGTTTCAG GTTTCAATTC ATCCAACTGG 
AATGGTGAAG CTACTTGTGC TCTAGGGATG CAAACAAGAA AAATCATTTG TGTTCGAGTC 
AACGTGGGAC AGGTTGGACC CAAAAAATGT CCTGAAAGCC TTCGTCCAGA AACCATGAGG 
CCATGTCTGC TTCCATGTCG AAAGGATTGC ATAGTTACAC CATATAGTGA CTGGGCCACT 
TGCCCCACCG CCTGTCAAGA CGGTGATGTT GCCATAAAAA AACAGACTCG TCATCGTGTC 
ATTATCCAAC TCCCATCTTA TGGAGGCCGT GACTGCCCCG ATACTTTATA CGAAGAAAAT 
GAATGCGAAG CTCCAGCAAC CTGTCACAGC TACCGCTGGA AAACACATAA ATGGAGAAGA 
TGCCAGTTGG TGCCGTGGTA CGTCCGACAA GACAGCCCTG GAGCTTATGA AACGTGTGGG 
CCTGGATTAC AAGCCAGAGC AATTACTTGC CGCAGACAAG ATGGTGCACA GGTTGATGTC 
ACTGATTGTC TTGCCAAAGC TGGTCCAATG CCTCCTTTAA CCCAGACCTG CCAGATACCT 
TGCCAAGATG ACTGTCAATT TACTAACTGG TCGAAATTTT CTTCATGTAA TGGTGACTGT 
GGTGCAGTAA GGACAAGAAA GCGATCACTT CTTGGGAAAA GTAAGAAAAA AGACAAGTGC 
AAGAACGCCC AGCTCTACCC AGTGATTGAG ACTCAGTTCT GCCCATGTGA TAAATACAGT 
GCTCAGCCTG TGGGAAACTG GTCAGACTGC ATCTTGCCAG AAGGAAAAGT GGAGGCCTTA 
CTGGGGGTAA AAGTACAAGG AGACATCAAG GAGTGTGGGC AGGGCTATCG TTACCAAGCC 
ATGGCATGTT ATGATCAAAA CGGCAGACTT GTGGAGACTT CAAGGTGTAA CAGCCATGGT 
TATATTGAGG AAGCATGTAT CATACCCTGT CCATCTGATT GTAAGCTTAG CGAATGGTCC 
AACTGGTCAC GGTGTAGCAA GTCCTGTGGG AGTGGAGTCA AGGTTCGGTC GAAATGGCTC 
CGTGAAAAAC CTTATAATGG TGGAAGACCT TGTCCTAAAC TGGATCACGT AAACCAGGCT 
CAGGTATATG AAGTGGTTCC ATGTCACAGT GATTGCAATC AGTATATATG GGTCACAGAG 
CCTTGGAGTA TTTGCAAAGT TACTAATGTT GATCTAAAGC AAAACTGCGG CGAAGGTGTT 
CAGACAAGAA AAGTAAGATG CATGCAGAAC ACAGTAGATG GCCCTTCGGA TGTTGTGGAA 
GACTATCTAT GTGAACCTGA GGAAATGCCA TTGGGCGCCA GGAACTGCAA GCTGCCCTGT 
CCTGAAGACT GTGTGATGTC TGAATGGAGT GAATGGAGCA GGTGTCCCTT GCCCTGTGAC 
GGAAGCAGCT ACCGAGAGAG GTCAGCCCTA CCTATCCGAC AAGCCGAGGA AGGCAAAGCC 
TGCCATGTTG CAATTGAAAC GGAGCCCTGC AACCTGAATA AAAACTGTTA TCACTATGAA 
TACAATGTCA CGGACTGGAG CACTTGCCAG CTAAGTGAGA AGGCAGTTTG TGGAAACGGG 
ATAAAAACAA GAATGCTGGA CTGCGTCCGC AGCGATGTCA AATCAGTTGA CTTGAAATAC 
TGTGAAGAGC TTGGCTTGGA AAAGACATGG CAGATGAATA TTTCTTGTGT GGTGGAATGT 
CCTATCAACT GTCAACTCTC CGAATGGTCA CCATGGTCCA AATGTTCCAA AGACTGTGGC 
CTAGCAGGTA AAATGTTACG GAATAGAACA GTGATTCAGC CGTCCCAGGG TGATGGAAGA 
CCTTGCCCTT CACAGATGGA GCAATCCAAA CCCTGCCCAG TAAAGCCATG TTACCGGTGG 
CAGTATGGAC GGTGGTCTTT GTGCAAAGTT GAGGATGCTC AGTGTGGTGT AGGAACAAGG 
TTCAGAAACA TTTCTTGTGT AGTGCATGAT GGATCCAGGG AAGATTCTGG TAAAATAGTA 
GACGAGGAGT TTTGCGGAGA CATTGACCTT ACTGTAAATA ATGACAAGAA CATTGTGCTA 
GAGGAATCAT GCACCGTTCC ATGCCCAGGT GATTGTTACT TGAGCGAATG GTCTGAGTGG 
AGTTTGTGTC AGCTGACTTG TGTTAATGGA GAAGATCTTG GCTTTGGAGG AATAAAGATG 
CGATCGCGTG CAGTAATTCT TCAAGAAATG GAAAATCAAC ATCTGTGTCC TGAACAAGTT 
GTGGAATCAC AACCATGTAA TGATGGAAAG TGTTATGAAT ATAAATGGCT GTCAAGTCCT 
TGGAAAGGAT CTTCTCGAAT GGTCTGGTGT CAGAGGTCAG ATGGCTTAAA TGTTACAGGA 
GGCTGTTTAG AGGCTTATCA TCCAGAAACT GAAAGATCGT GTAATCCACC TTGCACGCAG 
CAACACTCAT ATTGCACCGA GACAGGGTCA TGTGGCTGTG AAGATGGATA TACTGAAGTG 
ACATCGTCTG ATGGAACACT AGATCAGTGT ACTCTGATCC CAGTGGTGAT AATGCCAACA 
TTAGATGAAA AAAAGGGAGA CGTTAAAACG AGTCGTGCCA TCAACCCAAC CCAGCCATCC 
AGTAGTCAGT CGGGTTATGG AGGAAGAACA TGGTTTTTAC AACCCTTCGG TTCAGCAAAA 
AGCCGAAGAA ACCCCCGAGA CGACAGAACA ACCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821050.1
CDS	331..5169
Translation	MGRSTGFSGTLASSLGVIVLLLGGIWAAETQSESETKTIYIWKTGPWGRCMGDECGPGGIQTRAVWCAHVEGWTTLPTNCKQTDRPENQQNCFRVCDWHKEMYDWQLGSWNQCKPVISRSSEKPTECTKGEEGIQTREVTCIQKANGAVADDSICEYFEPKPRLEQGCLIPCPQDCIVSEYSSWSECSRTCGNGLKHRTRYVITLAQFGGSACPNLTEYQTCQSAPCEGEENMYSLSVGPWSSCSIPLSRHVRQTGKRGKNRERAKEREKAVKDPEARELIKKKRNRNRKNRQENRYWDIQIGYQTRLVTCMHRNGKTAALSFCQQEKLPITFQSCVIDKECQVSEWAEWSPCSRACDMESPGGYRTRKRTIKLFPIGNGNECPELEETEDCTPPPDGPQVCIKYTWRATEWTECRVDPLLSQQDKRRGNQTALCGGGIQTREIYCVQANENLLSYLNNVKDKEESHSYSTSKSSAAPFLSITFEISASKPVDPKLCSESPLSTSQLCHVPCPIECEVSPWSAWGPCTFENCEDQQSKKGFKLRKRNITNEPTGGTGNCPHLVEAIPCEEPSCYDWRLDKLEECIPDNGNNCGAGTQVPVVTCINSDGEEVDRQQCRDAIYPIPILCEVPCPKDCVLSMWSEWSSCSHTCSGKTTEGRQTRTRSILAYAGDGRSPCPESNALQEIRSCNEHPCTVYHWQTGPWGQCTEDVTVSGFNSSNWNGEATCALGMQTRKIICVRVNVGQVGPKKCPESLRPETMRPCLLPCRKDCIVTPYSDWATCPTACQDGDVAIKKQTRHRVIIQLPSYGGRDCPDTLYEENECEAPATCHSYRWKTHKWRRCQLVPWYVRQDSPGAYETCGPGLQARAITCRRQDGAQVDVTDCLAKAGPMPPLTQTCQIPCQDDCQFTNWSKFSSCNGDCGAVRTRKRSLLGKSKKKDKCKNAQLYPVIETQFCPCDKYSAQPVGNWSDCILPEGKVEALLGVKVQGDIKECGQGYRYQAMACYDQNGRLVETSRCNSHGYIEEACIIPCPSDCKLSEWSNWSRCSKSCGSGVKVRSKWLREKPYNGGRPCPKLDHVNQAQVYEVVPCHSDCNQYIWVTEPWSICKVTNVDLKQNCGEGVQTRKVRCMQNTVDGPSDVVEDYLCEPEEMPLGARNCKLPCPEDCVMSEWSEWSRCPLPCDGSSYRERSALPIRQAEEGKACHVAIETEPCNLNKNCYHYEYNVTDWSTCQLSEKAVCGNGIKTRMLDCVRSDVKSVDLKYCEELGLEKTWQMNISCVVECPINCQLSEWSPWSKCSKDCGLAGKMLRNRTVIQPSQGDGRPCPSQMEQSKPCPVKPCYRWQYGRWSLCKVEDAQCGVGTRFRNISCVVHDGSREDSGKIVDEEFCGDIDLTVNNDKNIVLEESCTVPCPGDCYLSEWSEWSLCQLTCVNGEDLGFGGIKMRSRAVILQEMENQHLCPEQVVESQPCNDGKCYEYKWLSSPWKGSSRMVWCQRSDGLNVTGGCLEAYHPETERSCNPPCTQQHSYCTETGSCGCEDGYTEVTSSDGTLDQCTLIPVVIMPTLDEKKGDVKTSRAINPTQPSSSQSGYGGRTWFLQPFGSAKSRRNPRDDRTTA

Target ORF information:

RefSeq Version	XM_012965596.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis thrombospondin type 1 domain containing 7A (thsd7a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965596.2

ORF Insert Sequence:

ATGGGGAGAA GCACCGGCTT TTCTGGTACC CTTGCTTCTT CCCTGGGAGT GATTGTTCTT 
CTTCTCGGTG GGATCTGGGC TGCTGAGACG CAGAGTGAAT CTGAAACCAA AACTATATAC 
ATATGGAAGA CTGGTCCCTG GGGCAGATGT ATGGGCGATG AATGTGGACC TGGAGGTATT 
CAAACAAGGG CAGTTTGGTG TGCCCATGTC GAAGGATGGA CGACCCTCCC TACAAACTGT 
AAACAGACAG ACAGGCCCGA AAACCAACAG AACTGTTTTA GAGTTTGCGA CTGGCACAAG 
GAAATGTATG ACTGGCAGTT AGGAAGTTGG AATCAGTGTA AACCAGTTAT ATCCAGATCT 
TCAGAGAAAC CCACAGAATG TACTAAAGGT GAGGAAGGCA TTCAGACTAG AGAAGTAACA 
TGCATCCAGA AAGCAAATGG GGCAGTAGCT GATGATAGTA TATGTGAATA CTTTGAACCT 
AAGCCTCGAC TGGAACAAGG ATGCCTCATT CCCTGCCCTC AAGACTGCAT TGTATCAGAA 
TATTCTTCAT GGTCAGAATG CTCCAGAACA TGTGGCAATG GATTAAAACA CAGAACCCGA 
TATGTTATTA CTTTGGCCCA GTTTGGTGGA TCAGCATGTC CTAACCTTAC AGAATACCAA 
ACATGTCAGT CTGCCCCATG TGAGGGTGAA GAAAATATGT ACAGTCTGAG TGTTGGACCC 
TGGAGTTCTT GCTCAATTCC ACTTTCTAGA CATGTAAGAC AGACTGGTAA ACGAGGCAAA 
AATAGAGAGC GAGCGAAGGA AAGAGAAAAG GCAGTGAAGG ATCCAGAAGC ACGTGAGCTT 
ATTAAAAAGA AAAGAAACCG GAACAGAAAG AATAGACAGG AGAATAGATA TTGGGATATA 
CAGATTGGTT ACCAGACACG ACTAGTAACT TGTATGCACC GGAATGGAAA AACTGCTGCG 
TTAAGTTTTT GTCAACAGGA AAAGTTGCCA ATTACGTTCC AGTCTTGTGT TATTGACAAG 
GAGTGCCAAG TGTCTGAGTG GGCAGAATGG AGCCCTTGCT CCAGGGCTTG TGATATGGAA 
TCTCCTGGTG GATATCGTAC TAGGAAAAGG ACTATCAAAC TATTTCCAAT TGGGAATGGA 
AACGAATGTC CAGAGCTAGA AGAAACAGAA GATTGTACTC CTCCACCAGA CGGGCCACAA 
GTTTGTATTA AATACACCTG GAGAGCTACA GAATGGACTG AATGCCGTGT TGATCCTTTG 
TTAAGCCAGC AGGATAAAAG GAGAGGTAAC CAGACTGCTC TATGCGGCGG AGGAATTCAA 
ACACGGGAGA TTTACTGTGT ACAGGCAAAT GAAAATCTGC TTTCTTACCT CAACAATGTA 
AAAGATAAAG AAGAAAGCCA TTCTTACAGC ACATCAAAGT CTAGTGCGGC ACCATTTCTA 
TCAATCACTT TTGAAATATC AGCATCCAAG CCTGTTGATC CCAAATTGTG CTCGGAATCA 
CCGCTAAGTA CAAGCCAGCT CTGCCATGTT CCATGCCCTA TTGAGTGTGA AGTTTCTCCA 
TGGTCAGCAT GGGGACCTTG CACCTTTGAA AACTGTGAAG ACCAACAAAG CAAAAAAGGG 
TTCAAGCTCA GAAAGCGCAA TATTACCAAT GAGCCTACTG GTGGGACTGG CAATTGCCCT 
CACCTTGTAG AAGCCATTCC TTGTGAAGAA CCATCTTGCT ACGACTGGCG ATTGGATAAA 
CTTGAAGAGT GTATTCCTGA CAACGGAAAC AATTGTGGGG CAGGAACTCA GGTGCCTGTT 
GTTACATGCA TTAATAGTGA CGGAGAAGAA GTTGACAGAC AACAGTGCAG AGATGCCATC 
TATCCGATCC CAATTTTGTG TGAAGTTCCC TGCCCAAAGG ATTGTGTTCT CAGCATGTGG 
TCTGAATGGT CGTCCTGCTC ACACACCTGC TCTGGAAAAA CAACAGAAGG ACGGCAGACA 
CGGACGCGGT CTATTCTCGC TTACGCAGGT GATGGAAGAA GTCCGTGCCC AGAAAGCAAC 
GCTCTGCAAG AGATTCGTAG CTGTAATGAG CATCCTTGTA CAGTATATCA CTGGCAGACG 
GGTCCCTGGG GCCAGTGCAC TGAGGATGTC ACTGTTTCAG GTTTCAATTC ATCCAACTGG 
AATGGTGAAG CTACTTGTGC TCTAGGGATG CAAACAAGAA AAATCATTTG TGTTCGAGTC 
AACGTGGGAC AGGTTGGACC CAAAAAATGT CCTGAAAGCC TTCGTCCAGA AACCATGAGG 
CCATGTCTGC TTCCATGTCG AAAGGATTGC ATAGTTACAC CATATAGTGA CTGGGCCACT 
TGCCCCACCG CCTGTCAAGA CGGTGATGTT GCCATAAAAA AACAGACTCG TCATCGTGTC 
ATTATCCAAC TCCCATCTTA TGGAGGCCGT GACTGCCCCG ATACTTTATA CGAAGAAAAT 
GAATGCGAAG CTCCAGCAAC CTGTCACAGC TACCGCTGGA AAACACATAA ATGGAGAAGA 
TGCCAGTTGG TGCCGTGGTA CGTCCGACAA GACAGCCCTG GAGCTTATGA AACGTGTGGG 
CCTGGATTAC AAGCCAGAGC AATTACTTGC CGCAGACAAG ATGGTGCACA GGTTGATGTC 
ACTGATTGTC TTGCCAAAGC TGGTCCAATG CCTCCTTTAA CCCAGACCTG CCAGATACCT 
TGCCAAGATG ACTGTCAATT TACTAACTGG TCGAAATTTT CTTCATGTAA TGGTGACTGT 
GGTGCAGTAA GGACAAGAAA GCGATCACTT CTTGGGAAAA GTAAGAAAAA AGACAAGTGC 
AAGAACGCCC AGCTCTACCC AGTGATTGAG ACTCAGTTCT GCCCATGTGA TAAATACAGT 
GCTCAGCCTG TGGGAAACTG GTCAGACTGC ATCTTGCCAG AAGGAAAAGT GGAGGCCTTA 
CTGGGGGTAA AAGTACAAGG AGACATCAAG GAGTGTGGGC AGGGCTATCG TTACCAAGCC 
ATGGCATGTT ATGATCAAAA CGGCAGACTT GTGGAGACTT CAAGGTGTAA CAGCCATGGT 
TATATTGAGG AAGCATGTAT CATACCCTGT CCATCTGATT GTAAGCTTAG CGAATGGTCC 
AACTGGTCAC GGTGTAGCAA GTCCTGTGGG AGTGGAGTCA AGGTTCGGTC GAAATGGCTC 
CGTGAAAAAC CTTATAATGG TGGAAGACCT TGTCCTAAAC TGGATCACGT AAACCAGGCT 
CAGGTATATG AAGTGGTTCC ATGTCACAGT GATTGCAATC AGTATATATG GGTCACAGAG 
CCTTGGAGTA TTTGCAAAGT TACTAATGTT GATCTAAAGC AAAACTGCGG CGAAGGTGTT 
CAGACAAGAA AAGTAAGATG CATGCAGAAC ACAGTAGATG GCCCTTCGGA TGTTGTGGAA 
GACTATCTAT GTGAACCTGA GGAAATGCCA TTGGGCGCCA GGAACTGCAA GCTGCCCTGT 
CCTGAAGACT GTGTGATGTC TGAATGGAGT GAATGGAGCA GGTGTCCCTT GCCCTGTGAC 
GGAAGCAGCT ACCGAGAGAG GTCAGCCCTA CCTATCCGAC AAGCCGAGGA AGGCAAAGCC 
TGCCATGTTG CAATTGAAAC GGAGCCCTGC AACCTGAATA AAAACTGTTA TCACTATGAA 
TACAATGTCA CGGACTGGAG CACTTGCCAG CTAAGTGAGA AGGCAGTTTG TGGAAACGGG 
ATAAAAACAA GAATGCTGGA CTGCGTCCGC AGCGATGTCA AATCAGTTGA CTTGAAATAC 
TGTGAAGAGC TTGGCTTGGA AAAGACATGG CAGATGAATA TTTCTTGTGT GGTGGAATGT 
CCTATCAACT GTCAACTCTC CGAATGGTCA CCATGGTCCA AATGTTCCAA AGACTGTGGC 
CTAGCAGGTA AAATGTTACG GAATAGAACA GTGATTCAGC CGTCCCAGGG TGATGGAAGA 
CCTTGCCCTT CACAGATGGA GCAATCCAAA CCCTGCCCAG TAAAGCCATG TTACCGGTGG 
CAGTATGGAC GGTGGTCTTT GTGCAAAGTT GAGGATGCTC AGTGTGGTGT AGGAACAAGG 
TTCAGAAACA TTTCTTGTGT AGTGCATGAT GGATCCAGGG AAGATTCTGG TAAAATAGTA 
GACGAGGAGT TTTGCGGAGA CATTGACCTT ACTGTAAATA ATGACAAGAA CATTGTGCTA 
GAGGAATCAT GCACCGTTCC ATGCCCAGGT GATTGTTACT TGAGCGAATG GTCTGAGTGG 
AGTTTGTGTC AGCTGACTTG TGTTAATGGA GAAGATCTTG GCTTTGGAGG AATAAAGATG 
CGATCGCGTG CAGTAATTCT TCAAGAAATG GAAAATCAAC ATCTGTGTCC TGAACAAGTT 
GTGGAATCAC AACCATGTAA TGATGGAAAG TGTTATGAAT ATAAATGGCT GTCAAGTCCT 
TGGAAAGGAT CTTCTCGAAT GGTCTGGTGT CAGAGGTCAG ATGGCTTAAA TGTTACAGGA 
GGCTGTTTAG AGGCTTATCA TCCAGAAACT GAAAGATCGT GTAATCCACC TTGCACGCAG 
CAACACTCAT ATTGCACCGA GACAGGGTCA TGTGGCTGTG AAGATGGATA TACTGAAGTG 
ACATCGTCTG ATGGAACACT AGATCAGTGT ACTCTGATCC CAGTGGTGAT AATGCCAACA 
TTAGATGAAA AAAAGGGAGA CGTTAAAACG AGTCGTGCCA TCAACCCAAC CCAGCCATCC 
AGTAGTCAGT CGGGTTATGG AGGAAGAACA TGGTTTTTAC AACCCTTCGG TTCAGCAAAA 
AGCCGAAGAA ACCCCCGAGA CGACAGAACA ACCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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thsd7a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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