dph6 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Gene Symbol	dph6
Entrez Gene ID	100497052
Full Name	diphthamine biosynthesis 6
General protein information	Preferred Names diphthamine biosynthesis 6 Names diphthine--ammonia ligase DPH6 homolog
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004917268.3	XP_004917325.1	diphthine--ammonia ligase isoform X2
XM_018096829.1	XP_017952318.1	diphthine--ammonia ligase isoform X3
XM_002932836.5	XP_002932882.2	diphthine--ammonia ligase isoform X2
XM_002932836.4	XP_002932882.1	diphthine--ammonia ligase isoform X1
XM_031891249.1	XP_031747109.1	diphthine--ammonia ligase isoform X1
XM_031891250.1	XP_031747110.1	diphthine--ammonia ligase isoform X3

Pathways from BioSystems

Homologies

	ATPBD4	XP_001089692.2
Drosophila melanogaster (fruit fly)	CG1578	NP_572749.2
Kluyveromyces lactis	KLLA0D16984g	XP_453811.1
Eremothecium gossypii	AGOS_AGL164W	NP_986503.1
Schizosaccharomyces pombe (fission yeast)	mug71	NP_595310.1
Magnaporthe oryzae (rice blast fungus)	MGG_00500	XP_003718561.1
Pan troglodytes (chimpanzee)	ATPBD4	XP_001135595.2
Canis lupus familiaris (dog)	DPH6	XP_005638265.1
Mus musculus (house mouse)	Dph6	NP_079951.1
Rattus norvegicus (Norway rat)	Dph6	NP_001014203.1
Saccharomyces cerevisiae (baker's yeast)	DPH6	NP_013244.1
Neurospora crassa	NCU02670	XP_965443.2
Xenopus tropicalis (tropical clawed frog)	dph6	XP_002932882.1
Homo sapiens (human)	DPH6	NP_542381.1
Bos taurus (cattle)	DPH6	NP_001070484.1
Danio rerio (zebrafish)	dph6	NP_001013308.2
Caenorhabditis elegans (roundworm)	E01A2.5	NP_491303.3
Gallus gallus (chicken)	ATPBD4	NP_001186639.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001943	XP_321119.5

Gene Ontology

The following dph6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dph6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa15029	XM_002932836.4 Latest version!	Xenopus tropicalis diphthamine biosynthesis 6 (dph6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa15030	XM_004917268.3 Latest version!	Xenopus tropicalis diphthamine biosynthesis 6 (dph6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa46440	XM_018096829.1 Latest version!	Xenopus tropicalis diphthamine biosynthesis 6 (dph6), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OXa64497	XM_031891249.1 Latest version!	Xenopus tropicalis diphthamine biosynthesis 6 (dph6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa64498	XM_002932836.5 Latest version!	Xenopus tropicalis diphthamine biosynthesis 6 (dph6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa64499	XM_031891250.1 Latest version!	Xenopus tropicalis diphthamine biosynthesis 6 (dph6), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dph6 ( XM_002932836.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002932882.1 Xenopus tropicalis diphthamine biosynthesis 6 (dph6), transcript variant X1, mRNA.

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CloneID	OXa15029
Clone ID Related Accession (Same CDS sequence)	XM_002932836.4
Accession Version	XM_002932836.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	diphthine--ammonia ligase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002932836.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGAGTA GGAGGCGTGT GCGATCCGGT GACAGCATGA GGGTGGTAGC GCTAATCAGT 
GGCGGGAAGG ACAGCTGCTA TAACATGATG CAGTGTGTTT CTGCTGGCCA CCAGATTGTT 
GCCCTGGCAA ACCTGAAGCC TCCAGAAAGC ACAGCTGATG AGTTAGACAG TTACATGTAC 
CAGACTGTTG GGCATCATGC GTTAGAGCTG TATGCAGAAG CCATGGGACT GCCTATCTAT 
CGTGCCACTC TGCAGGGCAC CAGCTTGGAT ACAGGCAGAG CTTATGCCCC ACAGGAAGGG 
GACGAGGTGG AGGATCTATA TCGACTACTG AAGCTGGTGA AGGAAAAGGA GGCTGTGGAT 
TCTGTTTCTG TTGGGGCAAT CCTGTCTGAT TACCAGCGTG TCCGCGTGGA GAATGTGTGT 
CAGAGGCTCG GCCTCCAGCC TTTAGCTTTC CTGTGGCGTA GGAAGCAAGA AGAACTTCTG 
GATGAAATGA TCTCCTCGGG TCTGAAGGCC ATCCTTATCA AGGTAGCTGC TTTTGGGTTA 
GATCCAGACA AGCACCTGGG GAAGACACTG GAAGACATGA GGCCTTACCT TAAGCAGCTC 
TCTGACAAGT ATGGTGTCCA TGTTTGCGGA GAAGGAGGAG AGTATGAGAC GCTCACTTTA 
GACTGTCCTC TATTCAAGAA AAGAATTGTT GTGGATTCTG CGGAGGTGAC GATGCATTCT 
AATGATGCCT TCGCTCCTGT GGCTTATCTT CGATTATCGA AGCTTCACCT TGAGGATAAA 
ATTACCAACA CAGCAGTTAC ATTGCAAAGA GAGTGTCCCT GTAGTAATGG ATTCCCTGCA 
GAGCCTGCAT CCTGTGCCAC AAAGGAATGT GATAATTCTG AGGAACGGCC ATTGCCCTGG 
ATACCGAACA GTCTCCCTTC GGTGCCCATG TGTGAATCAA GAACTTGTTC AGCTCCATCA 
CAATGTGGAT TCCAGTGGAT CAGTGAGATT ACTGCCAGTG AGGGAAATGT CTTGGAGGCT 
TCCCACAGGG CACTCTCTTC ACTTAAAGAC CAAGTACAAG AGTTGGGCCT GCAGATGAGT 
GATGCTGTGC TTATACATCT TTATGTCAAG AGCATGGAGG ACTTTTCTGA TATAAATAGG 
GTCTATGGGA CACTCTTCCC ATCTGCTCCC CCAGCTCGGG TTTGTGTGCA GTGTTGTCTT 
CCCGGCAGTG CATTTTTCAA GATGGATGCC CTATTCTGCT GTCCTTCACT TGCAGACGCT 
AGCAGTGACT GTGTGCCGAA GAAGACGGCA ATGCATGTGC AGAGCATCTC TCACTGGGCC 
CCTGCTAACA TTGGGCCCTA CAGCCAGGCT GTGCAGGTTG GTCCTGGTTT ATTCTGTGCT 
GGACAAATTG CACTGAAACC CTGTACCATG CAGCTGGTAT CTGGGGGAGC TACAGTGGAA 
GCCCAGGTCT CCCTGTGCCA TGTGGAACGT GTCCTGGACG CCATTAACCT TGGTACCTCC 
TTGGCCCACG TGCTGCTTGT TCAATGCTAT GTAACAAATC GCTCCTACAT ACCCATCGCC 
TTAGCTGCTT GGGGTAGCAG AAATGCACAA GAGGCTGATA TTGTCCCTCT TACCGTGGTG 
GTAGTACCAA TGCTTCCCCG TGGTGCTTCA GTTGAGTGGC ATGTTATAGC TGCCGTGAGC 
GACCCAGCAG AAAGACAATA CTTCTCAGTA TGCCAGGAAA ATCTGGGCTA CCAGGTGATG 
CTTTATGGGG TGATATCCAG TTGTGTGTCC TGTGCCTCCC TCTCACTGTC CCTAAGCTTG 
GCCCCAAACA GAGCTGTCCA TGAGGTGCAG GACAGGAATG AAGCTAGCAG CCTTATTCAT 
AAAGCACTCA GCAGAGCCAC AGAGAAACTT CCGACAAACC CCCATCATCT TACAGCCCTG 
TGCTGCAGAG TGTTCTACAG GAGGGATGGC ACAGAGTCAC AGAGGCTACA GGAAGGGCTT 
CAGGGAGTTC TGGGAGAAGT CTGGCCTGAG AAACCTCCAG GTCTGGTACT GGTGCCTGTA 
ATGGACCTGC CTTGTAACCA GATCCTACAC TTGTCCTTCT GGCTTAGTCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002932882.1
CDS	108..2201
Translation	MRSRRRVRSGDSMRVVALISGGKDSCYNMMQCVSAGHQIVALANLKPPESTADELDSYMYQTVGHHALELYAEAMGLPIYRATLQGTSLDTGRAYAPQEGDEVEDLYRLLKLVKEKEAVDSVSVGAILSDYQRVRVENVCQRLGLQPLAFLWRRKQEELLDEMISSGLKAILIKVAAFGLDPDKHLGKTLEDMRPYLKQLSDKYGVHVCGEGGEYETLTLDCPLFKKRIVVDSAEVTMHSNDAFAPVAYLRLSKLHLEDKITNTAVTLQRECPCSNGFPAEPASCATKECDNSEERPLPWIPNSLPSVPMCESRTCSAPSQCGFQWISEITASEGNVLEASHRALSSLKDQVQELGLQMSDAVLIHLYVKSMEDFSDINRVYGTLFPSAPPARVCVQCCLPGSAFFKMDALFCCPSLADASSDCVPKKTAMHVQSISHWAPANIGPYSQAVQVGPGLFCAGQIALKPCTMQLVSGGATVEAQVSLCHVERVLDAINLGTSLAHVLLVQCYVTNRSYIPIALAAWGSRNAQEADIVPLTVVVVPMLPRGASVEWHVIAAVSDPAERQYFSVCQENLGYQVMLYGVISSCVSCASLSLSLSLAPNRAVHEVQDRNEASSLIHKALSRATEKLPTNPHHLTALCCRVFYRRDGTESQRLQEGLQGVLGEVWPEKPPGLVLVPVMDLPCNQILHLSFWLSL

Target ORF information:

RefSeq Version	XM_002932836.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diphthamine biosynthesis 6 (dph6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002932836.4

ORF Insert Sequence:

ATGCGGAGTA GGAGGCGTGT GCGATCCGGT GACAGCATGA GGGTGGTAGC GCTAATCAGT 
GGCGGGAAGG ACAGCTGCTA TAACATGATG CAGTGTGTTT CTGCTGGCCA CCAGATTGTT 
GCCCTGGCAA ACCTGAAGCC TCCAGAAAGC ACAGCTGATG AGTTAGACAG TTACATGTAC 
CAGACTGTTG GGCATCATGC GTTAGAGCTG TATGCAGAAG CCATGGGACT GCCTATCTAT 
CGTGCCACTC TGCAGGGCAC CAGCTTGGAT ACAGGCAGAG CTTATGCCCC ACAGGAAGGG 
GACGAGGTGG AGGATCTATA TCGACTACTG AAGCTGGTGA AGGAAAAGGA GGCTGTGGAT 
TCTGTTTCTG TTGGGGCAAT CCTGTCTGAT TACCAGCGTG TCCGCGTGGA GAATGTGTGT 
CAGAGGCTCG GCCTCCAGCC TTTAGCTTTC CTGTGGCGTA GGAAGCAAGA AGAACTTCTG 
GATGAAATGA TCTCCTCGGG TCTGAAGGCC ATCCTTATCA AGGTAGCTGC TTTTGGGTTA 
GATCCAGACA AGCACCTGGG GAAGACACTG GAAGACATGA GGCCTTACCT TAAGCAGCTC 
TCTGACAAGT ATGGTGTCCA TGTTTGCGGA GAAGGAGGAG AGTATGAGAC GCTCACTTTA 
GACTGTCCTC TATTCAAGAA AAGAATTGTT GTGGATTCTG CGGAGGTGAC GATGCATTCT 
AATGATGCCT TCGCTCCTGT GGCTTATCTT CGATTATCGA AGCTTCACCT TGAGGATAAA 
ATTACCAACA CAGCAGTTAC ATTGCAAAGA GAGTGTCCCT GTAGTAATGG ATTCCCTGCA 
GAGCCTGCAT CCTGTGCCAC AAAGGAATGT GATAATTCTG AGGAACGGCC ATTGCCCTGG 
ATACCGAACA GTCTCCCTTC GGTGCCCATG TGTGAATCAA GAACTTGTTC AGCTCCATCA 
CAATGTGGAT TCCAGTGGAT CAGTGAGATT ACTGCCAGTG AGGGAAATGT CTTGGAGGCT 
TCCCACAGGG CACTCTCTTC ACTTAAAGAC CAAGTACAAG AGTTGGGCCT GCAGATGAGT 
GATGCTGTGC TTATACATCT TTATGTCAAG AGCATGGAGG ACTTTTCTGA TATAAATAGG 
GTCTATGGGA CACTCTTCCC ATCTGCTCCC CCAGCTCGGG TTTGTGTGCA GTGTTGTCTT 
CCCGGCAGTG CATTTTTCAA GATGGATGCC CTATTCTGCT GTCCTTCACT TGCAGACGCT 
AGCAGTGACT GTGTGCCGAA GAAGACGGCA ATGCATGTGC AGAGCATCTC TCACTGGGCC 
CCTGCTAACA TTGGGCCCTA CAGCCAGGCT GTGCAGGTTG GTCCTGGTTT ATTCTGTGCT 
GGACAAATTG CACTGAAACC CTGTACCATG CAGCTGGTAT CTGGGGGAGC TACAGTGGAA 
GCCCAGGTCT CCCTGTGCCA TGTGGAACGT GTCCTGGACG CCATTAACCT TGGTACCTCC 
TTGGCCCACG TGCTGCTTGT TCAATGCTAT GTAACAAATC GCTCCTACAT ACCCATCGCC 
TTAGCTGCTT GGGGTAGCAG AAATGCACAA GAGGCTGATA TTGTCCCTCT TACCGTGGTG 
GTAGTACCAA TGCTTCCCCG TGGTGCTTCA GTTGAGTGGC ATGTTATAGC TGCCGTGAGC 
GACCCAGCAG AAAGACAATA CTTCTCAGTA TGCCAGGAAA ATCTGGGCTA CCAGGTGATG 
CTTTATGGGG TGATATCCAG TTGTGTGTCC TGTGCCTCCC TCTCACTGTC CCTAAGCTTG 
GCCCCAAACA GAGCTGTCCA TGAGGTGCAG GACAGGAATG AAGCTAGCAG CCTTATTCAT 
AAAGCACTCA GCAGAGCCAC AGAGAAACTT CCGACAAACC CCCATCATCT TACAGCCCTG 
TGCTGCAGAG TGTTCTACAG GAGGGATGGC ACAGAGTCAC AGAGGCTACA GGAAGGGCTT 
CAGGGAGTTC TGGGAGAAGT CTGGCCTGAG AAACCTCCAG GTCTGGTACT GGTGCCTGTA 
ATGGACCTGC CTTGTAACCA GATCCTACAC TTGTCCTTCT GGCTTAGTCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dph6 ( XM_004917268.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917325.1 Xenopus tropicalis diphthamine biosynthesis 6 (dph6), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa15030
Clone ID Related Accession (Same CDS sequence)	XM_004917268.3
Accession Version	XM_004917268.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	diphthine--ammonia ligase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004917268.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCAGT GTGTTTCTGC TGGCCACCAG ATTGTTGCCC TGGCAAACCT GAAGCCTCCA 
GAAAGCACAG CTGATGAGTT AGACAGTTAC ATGTACCAGA CTGTTGGGCA TCATGCGTTA 
GAGCTGTATG CAGAAGCCAT GGGACTGCCT ATCTATCGTG CCACTCTGCA GGGCACCAGC 
TTGGATACAG GCAGAGCTTA TGCCCCACAG GAAGGGGACG AGGTGGAGGA TCTATATCGA 
CTACTGAAGC TGGTGAAGGA AAAGGAGGCT GTGGATTCTG TTTCTGTTGG GGCAATCCTG 
TCTGATTACC AGCGTGTCCG CGTGGAGAAT GTGTGTCAGA GGCTCGGCCT CCAGCCTTTA 
GCTTTCCTGT GGCGTAGGAA GCAAGAAGAA CTTCTGGATG AAATGATCTC CTCGGGTCTG 
AAGGCCATCC TTATCAAGGT AGCTGCTTTT GGGTTAGATC CAGACAAGCA CCTGGGGAAG 
ACACTGGAAG ACATGAGGCC TTACCTTAAG CAGCTCTCTG ACAAGTATGG TGTCCATGTT 
TGCGGAGAAG GAGGAGAGTA TGAGACGCTC ACTTTAGACT GTCCTCTATT CAAGAAAAGA 
ATTGTTGTGG ATTCTGCGGA GGTGACGATG CATTCTAATG ATGCCTTCGC TCCTGTGGCT 
TATCTTCGAT TATCGAAGCT TCACCTTGAG GATAAAATTA CCAACACAGC AGTTACATTG 
CAAAGAGAGT GTCCCTGTAG TAATGGATTC CCTGCAGAGC CTGCATCCTG TGCCACAAAG 
GAATGTGATA ATTCTGAGGA ACGGCCATTG CCCTGGATAC CGAACAGTCT CCCTTCGGTG 
CCCATGTGTG AATCAAGAAC TTGTTCAGCT CCATCACAAT GTGGATTCCA GTGGATCAGT 
GAGATTACTG CCAGTGAGGG AAATGTCTTG GAGGCTTCCC ACAGGGCACT CTCTTCACTT 
AAAGACCAAG TACAAGAGTT GGGCCTGCAG ATGAGTGATG CTGTGCTTAT ACATCTTTAT 
GTCAAGAGCA TGGAGGACTT TTCTGATATA AATAGGGTCT ATGGGACACT CTTCCCATCT 
GCTCCCCCAG CTCGGGTTTG TGTGCAGTGT TGTCTTCCCG GCAGTGCATT TTTCAAGATG 
GATGCCCTAT TCTGCTGTCC TTCACTTGCA GACGCTAGCA GTGACTGTGT GCCGAAGAAG 
ACGGCAATGC ATGTGCAGAG CATCTCTCAC TGGGCCCCTG CTAACATTGG GCCCTACAGC 
CAGGCTGTGC AGGTTGGTCC TGGTTTATTC TGTGCTGGAC AAATTGCACT GAAACCCTGT 
ACCATGCAGC TGGTATCTGG GGGAGCTACA GTGGAAGCCC AGGTCTCCCT GTGCCATGTG 
GAACGTGTCC TGGACGCCAT TAACCTTGGT ACCTCCTTGG CCCACGTGCT GCTTGTTCAA 
TGCTATGTAA CAAATCGCTC CTACATACCC ATCGCCTTAG CTGCTTGGGG TAGCAGAAAT 
GCACAAGAGG CTGATATTGT CCCTCTTACC GTGGTGGTAG TACCAATGCT TCCCCGTGGT 
GCTTCAGTTG AGTGGCATGT TATAGCTGCC GTGAGCGACC CAGCAGAAAG ACAATACTTC 
TCAGTATGCC AGGAAAATCT GGGCTACCAG GTGATGCTTT ATGGGGTGAT ATCCAGTTGT 
GTGTCCTGTG CCTCCCTCTC ACTGTCCCTA AGCTTGGCCC CAAACAGAGC TGTCCATGAG 
GTGCAGGACA GGAATGAAGC TAGCAGCCTT ATTCATAAAG CACTCAGCAG AGCCACAGAG 
AAACTTCCGA CAAACCCCCA TCATCTTACA GCCCTGTGCT GCAGAGTGTT CTACAGGAGG 
GATGGCACAG AGTCACAGAG GCTACAGGAA GGGCTTCAGG GAGTTCTGGG AGAAGTCTGG 
CCTGAGAAAC CTCCAGGTCT GGTACTGGTG CCTGTAATGG ACCTGCCTTG TAACCAGATC 
CTACACTTGT CCTTCTGGCT TAGTCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917325.1
CDS	89..2098
Translation	MMQCVSAGHQIVALANLKPPESTADELDSYMYQTVGHHALELYAEAMGLPIYRATLQGTSLDTGRAYAPQEGDEVEDLYRLLKLVKEKEAVDSVSVGAILSDYQRVRVENVCQRLGLQPLAFLWRRKQEELLDEMISSGLKAILIKVAAFGLDPDKHLGKTLEDMRPYLKQLSDKYGVHVCGEGGEYETLTLDCPLFKKRIVVDSAEVTMHSNDAFAPVAYLRLSKLHLEDKITNTAVTLQRECPCSNGFPAEPASCATKECDNSEERPLPWIPNSLPSVPMCESRTCSAPSQCGFQWISEITASEGNVLEASHRALSSLKDQVQELGLQMSDAVLIHLYVKSMEDFSDINRVYGTLFPSAPPARVCVQCCLPGSAFFKMDALFCCPSLADASSDCVPKKTAMHVQSISHWAPANIGPYSQAVQVGPGLFCAGQIALKPCTMQLVSGGATVEAQVSLCHVERVLDAINLGTSLAHVLLVQCYVTNRSYIPIALAAWGSRNAQEADIVPLTVVVVPMLPRGASVEWHVIAAVSDPAERQYFSVCQENLGYQVMLYGVISSCVSCASLSLSLSLAPNRAVHEVQDRNEASSLIHKALSRATEKLPTNPHHLTALCCRVFYRRDGTESQRLQEGLQGVLGEVWPEKPPGLVLVPVMDLPCNQILHLSFWLSL

Target ORF information:

RefSeq Version	XM_004917268.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diphthamine biosynthesis 6 (dph6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917268.3

ORF Insert Sequence:

ATGATGCAGT GTGTTTCTGC TGGCCACCAG ATTGTTGCCC TGGCAAACCT GAAGCCTCCA 
GAAAGCACAG CTGATGAGTT AGACAGTTAC ATGTACCAGA CTGTTGGGCA TCATGCGTTA 
GAGCTGTATG CAGAAGCCAT GGGACTGCCT ATCTATCGTG CCACTCTGCA GGGCACCAGC 
TTGGATACAG GCAGAGCTTA TGCCCCACAG GAAGGGGACG AGGTGGAGGA TCTATATCGA 
CTACTGAAGC TGGTGAAGGA AAAGGAGGCT GTGGATTCTG TTTCTGTTGG GGCAATCCTG 
TCTGATTACC AGCGTGTCCG CGTGGAGAAT GTGTGTCAGA GGCTCGGCCT CCAGCCTTTA 
GCTTTCCTGT GGCGTAGGAA GCAAGAAGAA CTTCTGGATG AAATGATCTC CTCGGGTCTG 
AAGGCCATCC TTATCAAGGT AGCTGCTTTT GGGTTAGATC CAGACAAGCA CCTGGGGAAG 
ACACTGGAAG ACATGAGGCC TTACCTTAAG CAGCTCTCTG ACAAGTATGG TGTCCATGTT 
TGCGGAGAAG GAGGAGAGTA TGAGACGCTC ACTTTAGACT GTCCTCTATT CAAGAAAAGA 
ATTGTTGTGG ATTCTGCGGA GGTGACGATG CATTCTAATG ATGCCTTCGC TCCTGTGGCT 
TATCTTCGAT TATCGAAGCT TCACCTTGAG GATAAAATTA CCAACACAGC AGTTACATTG 
CAAAGAGAGT GTCCCTGTAG TAATGGATTC CCTGCAGAGC CTGCATCCTG TGCCACAAAG 
GAATGTGATA ATTCTGAGGA ACGGCCATTG CCCTGGATAC CGAACAGTCT CCCTTCGGTG 
CCCATGTGTG AATCAAGAAC TTGTTCAGCT CCATCACAAT GTGGATTCCA GTGGATCAGT 
GAGATTACTG CCAGTGAGGG AAATGTCTTG GAGGCTTCCC ACAGGGCACT CTCTTCACTT 
AAAGACCAAG TACAAGAGTT GGGCCTGCAG ATGAGTGATG CTGTGCTTAT ACATCTTTAT 
GTCAAGAGCA TGGAGGACTT TTCTGATATA AATAGGGTCT ATGGGACACT CTTCCCATCT 
GCTCCCCCAG CTCGGGTTTG TGTGCAGTGT TGTCTTCCCG GCAGTGCATT TTTCAAGATG 
GATGCCCTAT TCTGCTGTCC TTCACTTGCA GACGCTAGCA GTGACTGTGT GCCGAAGAAG 
ACGGCAATGC ATGTGCAGAG CATCTCTCAC TGGGCCCCTG CTAACATTGG GCCCTACAGC 
CAGGCTGTGC AGGTTGGTCC TGGTTTATTC TGTGCTGGAC AAATTGCACT GAAACCCTGT 
ACCATGCAGC TGGTATCTGG GGGAGCTACA GTGGAAGCCC AGGTCTCCCT GTGCCATGTG 
GAACGTGTCC TGGACGCCAT TAACCTTGGT ACCTCCTTGG CCCACGTGCT GCTTGTTCAA 
TGCTATGTAA CAAATCGCTC CTACATACCC ATCGCCTTAG CTGCTTGGGG TAGCAGAAAT 
GCACAAGAGG CTGATATTGT CCCTCTTACC GTGGTGGTAG TACCAATGCT TCCCCGTGGT 
GCTTCAGTTG AGTGGCATGT TATAGCTGCC GTGAGCGACC CAGCAGAAAG ACAATACTTC 
TCAGTATGCC AGGAAAATCT GGGCTACCAG GTGATGCTTT ATGGGGTGAT ATCCAGTTGT 
GTGTCCTGTG CCTCCCTCTC ACTGTCCCTA AGCTTGGCCC CAAACAGAGC TGTCCATGAG 
GTGCAGGACA GGAATGAAGC TAGCAGCCTT ATTCATAAAG CACTCAGCAG AGCCACAGAG 
AAACTTCCGA CAAACCCCCA TCATCTTACA GCCCTGTGCT GCAGAGTGTT CTACAGGAGG 
GATGGCACAG AGTCACAGAG GCTACAGGAA GGGCTTCAGG GAGTTCTGGG AGAAGTCTGG 
CCTGAGAAAC CTCCAGGTCT GGTACTGGTG CCTGTAATGG ACCTGCCTTG TAACCAGATC 
CTACACTTGT CCTTCTGGCT TAGTCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dph6 ( XM_018096829.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952318.1 Xenopus tropicalis diphthamine biosynthesis 6 (dph6), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa46440
Clone ID Related Accession (Same CDS sequence)	XM_018096829.1
Accession Version	XM_018096829.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	diphthine--ammonia ligase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCAGA CTGTTGGGCA TCATGCGTTA GAGCTGTATG CAGAAGCCAT GGGACTGCCT 
ATCTATCGTG CCACTCTGCA GGGCACCAGC TTGGATACAG GCAGAGCTTA TGCCCCACAG 
GAAGGGGACG AGGTGGAGGA TCTATATCGA CTACTGAAGC TGGTGAAGGA AAAGGAGGCT 
GTGGATTCTG TTTCTGTTGG GGCAATCCTG TCTGATTACC AGCGTGTCCG CGTGGAGAAT 
GTGTGTCAGA GGCTCGGCCT CCAGCCTTTA GCTTTCCTGT GGCGTAGGAA GCAAGAAGAA 
CTTCTGGATG AAATGATCTC CTCGGGTCTG AAGGCCATCC TTATCAAGGT AGCTGCTTTT 
GGGTTAGATC CAGACAAGCA CCTGGGGAAG ACACTGGAAG ACATGAGGCC TTACCTTAAG 
CAGCTCTCTG ACAAGTATGG TGTCCATGTT TGCGGAGAAG GAGGAGAGTA TGAGACGCTC 
ACTTTAGACT GTCCTCTATT CAAGAAAAGA ATTGTTGTGG ATTCTGCGGA GGTGACGATG 
CATTCTAATG ATGCCTTCGC TCCTGTGGCT TATCTTCGAT TATCGAAGCT TCACCTTGAG 
GATAAAATTA CCAACACAGC AGTTACATTG CAAAGAGAGT GTCCCTGTAG TAATGGATTC 
CCTGCAGAGC CTGCATCCTG TGCCACAAAG GAATGTGATA ATTCTGAGGA ACGGCCATTG 
CCCTGGATAC CGAACAGTCT CCCTTCGGTG CCCATGTGTG AATCAAGAAC TTGTTCAGCT 
CCATCACAAT GTGGATTCCA GTGGATCAGT GAGATTACTG CCAGTGAGGG AAATGTCTTG 
GAGGCTTCCC ACAGGGCACT CTCTTCACTT AAAGACCAAG TACAAGAGTT GGGCCTGCAG 
ATGAGTGATG CTGTGCTTAT ACATCTTTAT GTCAAGAGCA TGGAGGACTT TTCTGATATA 
AATAGGGTCT ATGGGACACT CTTCCCATCT GCTCCCCCAG CTCGGGTTTG TGTGCAGTGT 
TGTCTTCCCG GCAGTGCATT TTTCAAGATG GATGCCCTAT TCTGCTGTCC TTCACTTGCA 
GACGCTAGCA GTGACTGTGT GCCGAAGAAG ACGGCAATGC ATGTGCAGAG CATCTCTCAC 
TGGGCCCCTG CTAACATTGG GCCCTACAGC CAGGCTGTGC AGGTTGGTCC TGGTTTATTC 
TGTGCTGGAC AAATTGCACT GAAACCCTGT ACCATGCAGC TGGTATCTGG GGGAGCTACA 
GTGGAAGCCC AGGTCTCCCT GTGCCATGTG GAACGTGTCC TGGACGCCAT TAACCTTGGT 
ACCTCCTTGG CCCACGTGCT GCTTGTTCAA TGCTATGTAA CAAATCGCTC CTACATACCC 
ATCGCCTTAG CTGCTTGGGG TAGCAGAAAT GCACAAGAGG CTGATATTGT CCCTCTTACC 
GTGGTGGTAG TACCAATGCT TCCCCGTGGT GCTTCAGTTG AGTGGCATGT TATAGCTGCC 
GTGAGCGACC CAGCAGAAAG ACAATACTTC TCAGTATGCC AGGAAAATCT GGGCTACCAG 
GTGATGCTTT ATGGGGTGAT ATCCAGTTGT GTGTCCTGTG CCTCCCTCTC ACTGTCCCTA 
AGCTTGGCCC CAAACAGAGC TGTCCATGAG GTGCAGGACA GGAATGAAGC TAGCAGCCTT 
ATTCATAAAG CACTCAGCAG AGCCACAGAG AAACTTCCGA CAAACCCCCA TCATCTTACA 
GCCCTGTGCT GCAGAGTGTT CTACAGGAGG GATGGCACAG AGTCACAGAG GCTACAGGAA 
GGGCTTCAGG GAGTTCTGGG AGAAGTCTGG CCTGAGAAAC CTCCAGGTCT GGTACTGGTG 
CCTGTAATGG ACCTGCCTTG TAACCAGATC CTACACTTGT CCTTCTGGCT TAGTCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952318.1
CDS	188..2107
Translation	MYQTVGHHALELYAEAMGLPIYRATLQGTSLDTGRAYAPQEGDEVEDLYRLLKLVKEKEAVDSVSVGAILSDYQRVRVENVCQRLGLQPLAFLWRRKQEELLDEMISSGLKAILIKVAAFGLDPDKHLGKTLEDMRPYLKQLSDKYGVHVCGEGGEYETLTLDCPLFKKRIVVDSAEVTMHSNDAFAPVAYLRLSKLHLEDKITNTAVTLQRECPCSNGFPAEPASCATKECDNSEERPLPWIPNSLPSVPMCESRTCSAPSQCGFQWISEITASEGNVLEASHRALSSLKDQVQELGLQMSDAVLIHLYVKSMEDFSDINRVYGTLFPSAPPARVCVQCCLPGSAFFKMDALFCCPSLADASSDCVPKKTAMHVQSISHWAPANIGPYSQAVQVGPGLFCAGQIALKPCTMQLVSGGATVEAQVSLCHVERVLDAINLGTSLAHVLLVQCYVTNRSYIPIALAAWGSRNAQEADIVPLTVVVVPMLPRGASVEWHVIAAVSDPAERQYFSVCQENLGYQVMLYGVISSCVSCASLSLSLSLAPNRAVHEVQDRNEASSLIHKALSRATEKLPTNPHHLTALCCRVFYRRDGTESQRLQEGLQGVLGEVWPEKPPGLVLVPVMDLPCNQILHLSFWLSL

Target ORF information:

RefSeq Version	XM_018096829.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diphthamine biosynthesis 6 (dph6), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096829.1

ORF Insert Sequence:

ATGTACCAGA CTGTTGGGCA TCATGCGTTA GAGCTGTATG CAGAAGCCAT GGGACTGCCT 
ATCTATCGTG CCACTCTGCA GGGCACCAGC TTGGATACAG GCAGAGCTTA TGCCCCACAG 
GAAGGGGACG AGGTGGAGGA TCTATATCGA CTACTGAAGC TGGTGAAGGA AAAGGAGGCT 
GTGGATTCTG TTTCTGTTGG GGCAATCCTG TCTGATTACC AGCGTGTCCG CGTGGAGAAT 
GTGTGTCAGA GGCTCGGCCT CCAGCCTTTA GCTTTCCTGT GGCGTAGGAA GCAAGAAGAA 
CTTCTGGATG AAATGATCTC CTCGGGTCTG AAGGCCATCC TTATCAAGGT AGCTGCTTTT 
GGGTTAGATC CAGACAAGCA CCTGGGGAAG ACACTGGAAG ACATGAGGCC TTACCTTAAG 
CAGCTCTCTG ACAAGTATGG TGTCCATGTT TGCGGAGAAG GAGGAGAGTA TGAGACGCTC 
ACTTTAGACT GTCCTCTATT CAAGAAAAGA ATTGTTGTGG ATTCTGCGGA GGTGACGATG 
CATTCTAATG ATGCCTTCGC TCCTGTGGCT TATCTTCGAT TATCGAAGCT TCACCTTGAG 
GATAAAATTA CCAACACAGC AGTTACATTG CAAAGAGAGT GTCCCTGTAG TAATGGATTC 
CCTGCAGAGC CTGCATCCTG TGCCACAAAG GAATGTGATA ATTCTGAGGA ACGGCCATTG 
CCCTGGATAC CGAACAGTCT CCCTTCGGTG CCCATGTGTG AATCAAGAAC TTGTTCAGCT 
CCATCACAAT GTGGATTCCA GTGGATCAGT GAGATTACTG CCAGTGAGGG AAATGTCTTG 
GAGGCTTCCC ACAGGGCACT CTCTTCACTT AAAGACCAAG TACAAGAGTT GGGCCTGCAG 
ATGAGTGATG CTGTGCTTAT ACATCTTTAT GTCAAGAGCA TGGAGGACTT TTCTGATATA 
AATAGGGTCT ATGGGACACT CTTCCCATCT GCTCCCCCAG CTCGGGTTTG TGTGCAGTGT 
TGTCTTCCCG GCAGTGCATT TTTCAAGATG GATGCCCTAT TCTGCTGTCC TTCACTTGCA 
GACGCTAGCA GTGACTGTGT GCCGAAGAAG ACGGCAATGC ATGTGCAGAG CATCTCTCAC 
TGGGCCCCTG CTAACATTGG GCCCTACAGC CAGGCTGTGC AGGTTGGTCC TGGTTTATTC 
TGTGCTGGAC AAATTGCACT GAAACCCTGT ACCATGCAGC TGGTATCTGG GGGAGCTACA 
GTGGAAGCCC AGGTCTCCCT GTGCCATGTG GAACGTGTCC TGGACGCCAT TAACCTTGGT 
ACCTCCTTGG CCCACGTGCT GCTTGTTCAA TGCTATGTAA CAAATCGCTC CTACATACCC 
ATCGCCTTAG CTGCTTGGGG TAGCAGAAAT GCACAAGAGG CTGATATTGT CCCTCTTACC 
GTGGTGGTAG TACCAATGCT TCCCCGTGGT GCTTCAGTTG AGTGGCATGT TATAGCTGCC 
GTGAGCGACC CAGCAGAAAG ACAATACTTC TCAGTATGCC AGGAAAATCT GGGCTACCAG 
GTGATGCTTT ATGGGGTGAT ATCCAGTTGT GTGTCCTGTG CCTCCCTCTC ACTGTCCCTA 
AGCTTGGCCC CAAACAGAGC TGTCCATGAG GTGCAGGACA GGAATGAAGC TAGCAGCCTT 
ATTCATAAAG CACTCAGCAG AGCCACAGAG AAACTTCCGA CAAACCCCCA TCATCTTACA 
GCCCTGTGCT GCAGAGTGTT CTACAGGAGG GATGGCACAG AGTCACAGAG GCTACAGGAA 
GGGCTTCAGG GAGTTCTGGG AGAAGTCTGG CCTGAGAAAC CTCCAGGTCT GGTACTGGTG 
CCTGTAATGG ACCTGCCTTG TAACCAGATC CTACACTTGT CCTTCTGGCT TAGTCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dph6 ( XM_031891249.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031747109.1 Xenopus tropicalis diphthamine biosynthesis 6 (dph6), transcript variant X1, mRNA.

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CloneID	OXa64497
Clone ID Related Accession (Same CDS sequence)	XM_031891249.1
Accession Version	XM_031891249.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	diphthine--ammonia ligase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGAGTA GGAGGCGTGT GCGATCCGGT GACAGCATGA GGGTGGTAGC GCTAATCAGT 
GGCGGGAAGG ACAGCTGCTA TAACATGATG CAGTGTGTTT CTGCTGGCCA CCAGATTGTT 
GCCCTGGCAA ACCTGAAGCC TCCAGAAAGC ACAGCTGATG AGTTAGACAG TTACATGTAC 
CAGACTGTTG GGCATCATGC GTTAGAGCTG TATGCAGAAG CCATGGGACT GCCTATCTAT 
CGTGCCACTC TGCAGGGCAC CAGCTTGGAT ACAGGCAGAG CTTATGCCCC ACAGGAAGGG 
GACGAGGTGG AGGATCTATA TCGACTACTG AAGCTGGTGA AGGAAAAGGA GGCTGTGGAT 
TCTGTTTCTG TTGGGGCAAT CCTGTCTGAT TACCAGCGTG TCCGCGTGGA GAATGTGTGT 
CAGAGGCTCG GCCTCCAGCC TTTAGCTTTC CTGTGGCGTA GGAAGCAAGA AGAACTTCTG 
GATGAAATGA TCTCCTCGGG TCTGAAGGCC ATCCTTATCA AGGTAGCTGC TTTTGGGTTA 
GATCCAGACA AGCACCTGGG GAAGACACTG GAAGACATGA GGCCTTACCT TAAGCAGCTC 
TCTGACAAGT ATGGTGTCCA TGTTTGCGGA GAAGGAGGAG AGTATGAGAC GCTCACTTTA 
GACTGTCCTC TATTCAAGAA AAGAATTGTT GTGGATTCTG CGGAGGTGAC GATGCATTCT 
AATGATGCCT TCGCTCCTGT GGCTTATCTT CGATTATCGA AGCTTCACCT TGAGGATAAA 
ATTACCAACA CAGCAGTTAC ATTGCAAAGA GAGTGTCCCT GTAGTAATGG ATTCCCTGCA 
GAGCCTGCAT CCTGTGCCAC AAAGGAATGT GATAATTCTG AGGAACGGCC ATTGCCCTGG 
ATACCGAACA GTCTCCCTTC GGTGCCCATG TTCTGTTTGC CTGTAGGTGA ATCAAGAACT 
TGTTCAGCTC CATCACAATG TGGATTCCAG TGGATCAGTG AGATTACTGC CAGTGAGGGA 
AATGTCTTGG AGGCTTCCCA CAGGGCACTC TCTTCACTTA AAGACCAAGT ACAAGAGTTG 
GGCCTGCAGA TGAGTGATGC TGTGCTTATA CATCTTTATG TCAAGAGCAT GGAGGACTTT 
TCTGATATAA ATAGGGTCTA TGGGACACTC TTCCCATCTG CTCCCCCAGC TCGGGTTTGT 
GTGCAGTGTT GTCTTCCCGG CAGTGCATTT TTCAAGATGG ATGCCCTATT CTGCTGTCCT 
TCACTTGCAG ACGCTAGCAG TGACTGTGTG CCGAAGAAGA CGGCAATGCA TGTGCAGAGC 
ATCTCTCACT GGGCCCCTGC TAACATTGGG CCCTACAGCC AGGCTGTGCA GGTTGGTCCT 
GGTTTATTCT GTGCTGGACA AATTGCACTG AAACCCTGTA CCATGCAGCT GGTATCTGGG 
GGAGCTACAG TGGAAGCCCA GGTCTCCCTG TGCCATGTGG AACGTGTCCT GGACGCCATT 
AACCTTGGTA CCTCCTTGGC CCACGTGCTG CTTGTTCAAT GCTATGTAAC AAATCGCTCC 
TACATACCCA TCGCCTTAGC TGCTTGGGGT AGCAGAAATG CACAAGAGGC TGATATTGTC 
CCTCTTACCG TGGTGGTAGT ACCAATGCTT CCCCGTGGTG CTTCAGTTGA GTGGCATGTT 
ATAGCTGCCG TGAGCGACCC AGCAGAAAGA CAACACTTCT CAGTATGCCA GGAAAATCTG 
GGCTACCAGG TGATGCTTTA TGGGGTGATA TCCAGTTGTG TGTCCTGTGC CTCCCTCTCA 
CTGTCCCTAA GCTTGGCCCC AAACAGAGCT GTCCATGAGG TGAAGGACAG GAATGAAGCT 
AGCAGCCTTA TTCATAAAGC ACTCAGCAGA GCCACAGAGA AACTTCCGAC AAACCCCCAT 
CATCTTACAG CCCTGTGCTG CAGAGTGTTC TACAGGAGGG ATGGCACAGA GTCACAGAGG 
CTACAGGAAG GGCTTCAGGG AGTTCTGGGA GAAGTCTGGC CTGAGAAACC TCCAGCTCTG 
GTACTGGTGC CTGTAATGGA CCTGCCTTGT AACCAGATCC TACACTTGTC CTTCTGGCTT 
AGTCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031747109.1
CDS	98..2206
Translation	MRSRRRVRSGDSMRVVALISGGKDSCYNMMQCVSAGHQIVALANLKPPESTADELDSYMYQTVGHHALELYAEAMGLPIYRATLQGTSLDTGRAYAPQEGDEVEDLYRLLKLVKEKEAVDSVSVGAILSDYQRVRVENVCQRLGLQPLAFLWRRKQEELLDEMISSGLKAILIKVAAFGLDPDKHLGKTLEDMRPYLKQLSDKYGVHVCGEGGEYETLTLDCPLFKKRIVVDSAEVTMHSNDAFAPVAYLRLSKLHLEDKITNTAVTLQRECPCSNGFPAEPASCATKECDNSEERPLPWIPNSLPSVPMFCLPVGESRTCSAPSQCGFQWISEITASEGNVLEASHRALSSLKDQVQELGLQMSDAVLIHLYVKSMEDFSDINRVYGTLFPSAPPARVCVQCCLPGSAFFKMDALFCCPSLADASSDCVPKKTAMHVQSISHWAPANIGPYSQAVQVGPGLFCAGQIALKPCTMQLVSGGATVEAQVSLCHVERVLDAINLGTSLAHVLLVQCYVTNRSYIPIALAAWGSRNAQEADIVPLTVVVVPMLPRGASVEWHVIAAVSDPAERQHFSVCQENLGYQVMLYGVISSCVSCASLSLSLSLAPNRAVHEVKDRNEASSLIHKALSRATEKLPTNPHHLTALCCRVFYRRDGTESQRLQEGLQGVLGEVWPEKPPALVLVPVMDLPCNQILHLSFWLSL

Target ORF information:

RefSeq Version	XM_031891249.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diphthamine biosynthesis 6 (dph6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891249.1

ORF Insert Sequence:

ATGCGGAGTA GGAGGCGTGT GCGATCCGGT GACAGCATGA GGGTGGTAGC GCTAATCAGT 
GGCGGGAAGG ACAGCTGCTA TAACATGATG CAGTGTGTTT CTGCTGGCCA CCAGATTGTT 
GCCCTGGCAA ACCTGAAGCC TCCAGAAAGC ACAGCTGATG AGTTAGACAG TTACATGTAC 
CAGACTGTTG GGCATCATGC GTTAGAGCTG TATGCAGAAG CCATGGGACT GCCTATCTAT 
CGTGCCACTC TGCAGGGCAC CAGCTTGGAT ACAGGCAGAG CTTATGCCCC ACAGGAAGGG 
GACGAGGTGG AGGATCTATA TCGACTACTG AAGCTGGTGA AGGAAAAGGA GGCTGTGGAT 
TCTGTTTCTG TTGGGGCAAT CCTGTCTGAT TACCAGCGTG TCCGCGTGGA GAATGTGTGT 
CAGAGGCTCG GCCTCCAGCC TTTAGCTTTC CTGTGGCGTA GGAAGCAAGA AGAACTTCTG 
GATGAAATGA TCTCCTCGGG TCTGAAGGCC ATCCTTATCA AGGTAGCTGC TTTTGGGTTA 
GATCCAGACA AGCACCTGGG GAAGACACTG GAAGACATGA GGCCTTACCT TAAGCAGCTC 
TCTGACAAGT ATGGTGTCCA TGTTTGCGGA GAAGGAGGAG AGTATGAGAC GCTCACTTTA 
GACTGTCCTC TATTCAAGAA AAGAATTGTT GTGGATTCTG CGGAGGTGAC GATGCATTCT 
AATGATGCCT TCGCTCCTGT GGCTTATCTT CGATTATCGA AGCTTCACCT TGAGGATAAA 
ATTACCAACA CAGCAGTTAC ATTGCAAAGA GAGTGTCCCT GTAGTAATGG ATTCCCTGCA 
GAGCCTGCAT CCTGTGCCAC AAAGGAATGT GATAATTCTG AGGAACGGCC ATTGCCCTGG 
ATACCGAACA GTCTCCCTTC GGTGCCCATG TTCTGTTTGC CTGTAGGTGA ATCAAGAACT 
TGTTCAGCTC CATCACAATG TGGATTCCAG TGGATCAGTG AGATTACTGC CAGTGAGGGA 
AATGTCTTGG AGGCTTCCCA CAGGGCACTC TCTTCACTTA AAGACCAAGT ACAAGAGTTG 
GGCCTGCAGA TGAGTGATGC TGTGCTTATA CATCTTTATG TCAAGAGCAT GGAGGACTTT 
TCTGATATAA ATAGGGTCTA TGGGACACTC TTCCCATCTG CTCCCCCAGC TCGGGTTTGT 
GTGCAGTGTT GTCTTCCCGG CAGTGCATTT TTCAAGATGG ATGCCCTATT CTGCTGTCCT 
TCACTTGCAG ACGCTAGCAG TGACTGTGTG CCGAAGAAGA CGGCAATGCA TGTGCAGAGC 
ATCTCTCACT GGGCCCCTGC TAACATTGGG CCCTACAGCC AGGCTGTGCA GGTTGGTCCT 
GGTTTATTCT GTGCTGGACA AATTGCACTG AAACCCTGTA CCATGCAGCT GGTATCTGGG 
GGAGCTACAG TGGAAGCCCA GGTCTCCCTG TGCCATGTGG AACGTGTCCT GGACGCCATT 
AACCTTGGTA CCTCCTTGGC CCACGTGCTG CTTGTTCAAT GCTATGTAAC AAATCGCTCC 
TACATACCCA TCGCCTTAGC TGCTTGGGGT AGCAGAAATG CACAAGAGGC TGATATTGTC 
CCTCTTACCG TGGTGGTAGT ACCAATGCTT CCCCGTGGTG CTTCAGTTGA GTGGCATGTT 
ATAGCTGCCG TGAGCGACCC AGCAGAAAGA CAACACTTCT CAGTATGCCA GGAAAATCTG 
GGCTACCAGG TGATGCTTTA TGGGGTGATA TCCAGTTGTG TGTCCTGTGC CTCCCTCTCA 
CTGTCCCTAA GCTTGGCCCC AAACAGAGCT GTCCATGAGG TGAAGGACAG GAATGAAGCT 
AGCAGCCTTA TTCATAAAGC ACTCAGCAGA GCCACAGAGA AACTTCCGAC AAACCCCCAT 
CATCTTACAG CCCTGTGCTG CAGAGTGTTC TACAGGAGGG ATGGCACAGA GTCACAGAGG 
CTACAGGAAG GGCTTCAGGG AGTTCTGGGA GAAGTCTGGC CTGAGAAACC TCCAGCTCTG 
GTACTGGTGC CTGTAATGGA CCTGCCTTGT AACCAGATCC TACACTTGTC CTTCTGGCTT 
AGTCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dph6 ( XM_002932836.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002932882.2 Xenopus tropicalis diphthamine biosynthesis 6 (dph6), transcript variant X2, mRNA.

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CloneID	OXa64498
Clone ID Related Accession (Same CDS sequence)	XM_002932836.5
Accession Version	XM_002932836.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	diphthine--ammonia ligase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002932836.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGAGTA GGAGGCGTGT GCGATCCGGT GACAGCATGA GGGTGGTAGC GCTAATCAGT 
GGCGGGAAGG ACAGCTGCTA TAACATGATG CAGTGTGTTT CTGCTGGCCA CCAGATTGTT 
GCCCTGGCAA ACCTGAAGCC TCCAGAAAGC ACAGCTGATG AGTTAGACAG TTACATGTAC 
CAGACTGTTG GGCATCATGC GTTAGAGCTG TATGCAGAAG CCATGGGACT GCCTATCTAT 
CGTGCCACTC TGCAGGGCAC CAGCTTGGAT ACAGGCAGAG CTTATGCCCC ACAGGAAGGG 
GACGAGGTGG AGGATCTATA TCGACTACTG AAGCTGGTGA AGGAAAAGGA GGCTGTGGAT 
TCTGTTTCTG TTGGGGCAAT CCTGTCTGAT TACCAGCGTG TCCGCGTGGA GAATGTGTGT 
CAGAGGCTCG GCCTCCAGCC TTTAGCTTTC CTGTGGCGTA GGAAGCAAGA AGAACTTCTG 
GATGAAATGA TCTCCTCGGG TCTGAAGGCC ATCCTTATCA AGGTAGCTGC TTTTGGGTTA 
GATCCAGACA AGCACCTGGG GAAGACACTG GAAGACATGA GGCCTTACCT TAAGCAGCTC 
TCTGACAAGT ATGGTGTCCA TGTTTGCGGA GAAGGAGGAG AGTATGAGAC GCTCACTTTA 
GACTGTCCTC TATTCAAGAA AAGAATTGTT GTGGATTCTG CGGAGGTGAC GATGCATTCT 
AATGATGCCT TCGCTCCTGT GGCTTATCTT CGATTATCGA AGCTTCACCT TGAGGATAAA 
ATTACCAACA CAGCAGTTAC ATTGCAAAGA GAGTGTCCCT GTAGTAATGG ATTCCCTGCA 
GAGCCTGCAT CCTGTGCCAC AAAGGAATGT GATAATTCTG AGGAACGGCC ATTGCCCTGG 
ATACCGAACA GTCTCCCTTC GGTGCCCATG TGTGAATCAA GAACTTGTTC AGCTCCATCA 
CAATGTGGAT TCCAGTGGAT CAGTGAGATT ACTGCCAGTG AGGGAAATGT CTTGGAGGCT 
TCCCACAGGG CACTCTCTTC ACTTAAAGAC CAAGTACAAG AGTTGGGCCT GCAGATGAGT 
GATGCTGTGC TTATACATCT TTATGTCAAG AGCATGGAGG ACTTTTCTGA TATAAATAGG 
GTCTATGGGA CACTCTTCCC ATCTGCTCCC CCAGCTCGGG TTTGTGTGCA GTGTTGTCTT 
CCCGGCAGTG CATTTTTCAA GATGGATGCC CTATTCTGCT GTCCTTCACT TGCAGACGCT 
AGCAGTGACT GTGTGCCGAA GAAGACGGCA ATGCATGTGC AGAGCATCTC TCACTGGGCC 
CCTGCTAACA TTGGGCCCTA CAGCCAGGCT GTGCAGGTTG GTCCTGGTTT ATTCTGTGCT 
GGACAAATTG CACTGAAACC CTGTACCATG CAGCTGGTAT CTGGGGGAGC TACAGTGGAA 
GCCCAGGTCT CCCTGTGCCA TGTGGAACGT GTCCTGGACG CCATTAACCT TGGTACCTCC 
TTGGCCCACG TGCTGCTTGT TCAATGCTAT GTAACAAATC GCTCCTACAT ACCCATCGCC 
TTAGCTGCTT GGGGTAGCAG AAATGCACAA GAGGCTGATA TTGTCCCTCT TACCGTGGTG 
GTAGTACCAA TGCTTCCCCG TGGTGCTTCA GTTGAGTGGC ATGTTATAGC TGCCGTGAGC 
GACCCAGCAG AAAGACAACA CTTCTCAGTA TGCCAGGAAA ATCTGGGCTA CCAGGTGATG 
CTTTATGGGG TGATATCCAG TTGTGTGTCC TGTGCCTCCC TCTCACTGTC CCTAAGCTTG 
GCCCCAAACA GAGCTGTCCA TGAGGTGAAG GACAGGAATG AAGCTAGCAG CCTTATTCAT 
AAAGCACTCA GCAGAGCCAC AGAGAAACTT CCGACAAACC CCCATCATCT TACAGCCCTG 
TGCTGCAGAG TGTTCTACAG GAGGGATGGC ACAGAGTCAC AGAGGCTACA GGAAGGGCTT 
CAGGGAGTTC TGGGAGAAGT CTGGCCTGAG AAACCTCCAG CTCTGGTACT GGTGCCTGTA 
ATGGACCTGC CTTGTAACCA GATCCTACAC TTGTCCTTCT GGCTTAGTCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002932882.2
CDS	97..2190
Translation	MRSRRRVRSGDSMRVVALISGGKDSCYNMMQCVSAGHQIVALANLKPPESTADELDSYMYQTVGHHALELYAEAMGLPIYRATLQGTSLDTGRAYAPQEGDEVEDLYRLLKLVKEKEAVDSVSVGAILSDYQRVRVENVCQRLGLQPLAFLWRRKQEELLDEMISSGLKAILIKVAAFGLDPDKHLGKTLEDMRPYLKQLSDKYGVHVCGEGGEYETLTLDCPLFKKRIVVDSAEVTMHSNDAFAPVAYLRLSKLHLEDKITNTAVTLQRECPCSNGFPAEPASCATKECDNSEERPLPWIPNSLPSVPMCESRTCSAPSQCGFQWISEITASEGNVLEASHRALSSLKDQVQELGLQMSDAVLIHLYVKSMEDFSDINRVYGTLFPSAPPARVCVQCCLPGSAFFKMDALFCCPSLADASSDCVPKKTAMHVQSISHWAPANIGPYSQAVQVGPGLFCAGQIALKPCTMQLVSGGATVEAQVSLCHVERVLDAINLGTSLAHVLLVQCYVTNRSYIPIALAAWGSRNAQEADIVPLTVVVVPMLPRGASVEWHVIAAVSDPAERQHFSVCQENLGYQVMLYGVISSCVSCASLSLSLSLAPNRAVHEVKDRNEASSLIHKALSRATEKLPTNPHHLTALCCRVFYRRDGTESQRLQEGLQGVLGEVWPEKPPALVLVPVMDLPCNQILHLSFWLSL

Target ORF information:

RefSeq Version	XM_002932836.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diphthamine biosynthesis 6 (dph6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002932836.5

ORF Insert Sequence:

ATGCGGAGTA GGAGGCGTGT GCGATCCGGT GACAGCATGA GGGTGGTAGC GCTAATCAGT 
GGCGGGAAGG ACAGCTGCTA TAACATGATG CAGTGTGTTT CTGCTGGCCA CCAGATTGTT 
GCCCTGGCAA ACCTGAAGCC TCCAGAAAGC ACAGCTGATG AGTTAGACAG TTACATGTAC 
CAGACTGTTG GGCATCATGC GTTAGAGCTG TATGCAGAAG CCATGGGACT GCCTATCTAT 
CGTGCCACTC TGCAGGGCAC CAGCTTGGAT ACAGGCAGAG CTTATGCCCC ACAGGAAGGG 
GACGAGGTGG AGGATCTATA TCGACTACTG AAGCTGGTGA AGGAAAAGGA GGCTGTGGAT 
TCTGTTTCTG TTGGGGCAAT CCTGTCTGAT TACCAGCGTG TCCGCGTGGA GAATGTGTGT 
CAGAGGCTCG GCCTCCAGCC TTTAGCTTTC CTGTGGCGTA GGAAGCAAGA AGAACTTCTG 
GATGAAATGA TCTCCTCGGG TCTGAAGGCC ATCCTTATCA AGGTAGCTGC TTTTGGGTTA 
GATCCAGACA AGCACCTGGG GAAGACACTG GAAGACATGA GGCCTTACCT TAAGCAGCTC 
TCTGACAAGT ATGGTGTCCA TGTTTGCGGA GAAGGAGGAG AGTATGAGAC GCTCACTTTA 
GACTGTCCTC TATTCAAGAA AAGAATTGTT GTGGATTCTG CGGAGGTGAC GATGCATTCT 
AATGATGCCT TCGCTCCTGT GGCTTATCTT CGATTATCGA AGCTTCACCT TGAGGATAAA 
ATTACCAACA CAGCAGTTAC ATTGCAAAGA GAGTGTCCCT GTAGTAATGG ATTCCCTGCA 
GAGCCTGCAT CCTGTGCCAC AAAGGAATGT GATAATTCTG AGGAACGGCC ATTGCCCTGG 
ATACCGAACA GTCTCCCTTC GGTGCCCATG TGTGAATCAA GAACTTGTTC AGCTCCATCA 
CAATGTGGAT TCCAGTGGAT CAGTGAGATT ACTGCCAGTG AGGGAAATGT CTTGGAGGCT 
TCCCACAGGG CACTCTCTTC ACTTAAAGAC CAAGTACAAG AGTTGGGCCT GCAGATGAGT 
GATGCTGTGC TTATACATCT TTATGTCAAG AGCATGGAGG ACTTTTCTGA TATAAATAGG 
GTCTATGGGA CACTCTTCCC ATCTGCTCCC CCAGCTCGGG TTTGTGTGCA GTGTTGTCTT 
CCCGGCAGTG CATTTTTCAA GATGGATGCC CTATTCTGCT GTCCTTCACT TGCAGACGCT 
AGCAGTGACT GTGTGCCGAA GAAGACGGCA ATGCATGTGC AGAGCATCTC TCACTGGGCC 
CCTGCTAACA TTGGGCCCTA CAGCCAGGCT GTGCAGGTTG GTCCTGGTTT ATTCTGTGCT 
GGACAAATTG CACTGAAACC CTGTACCATG CAGCTGGTAT CTGGGGGAGC TACAGTGGAA 
GCCCAGGTCT CCCTGTGCCA TGTGGAACGT GTCCTGGACG CCATTAACCT TGGTACCTCC 
TTGGCCCACG TGCTGCTTGT TCAATGCTAT GTAACAAATC GCTCCTACAT ACCCATCGCC 
TTAGCTGCTT GGGGTAGCAG AAATGCACAA GAGGCTGATA TTGTCCCTCT TACCGTGGTG 
GTAGTACCAA TGCTTCCCCG TGGTGCTTCA GTTGAGTGGC ATGTTATAGC TGCCGTGAGC 
GACCCAGCAG AAAGACAACA CTTCTCAGTA TGCCAGGAAA ATCTGGGCTA CCAGGTGATG 
CTTTATGGGG TGATATCCAG TTGTGTGTCC TGTGCCTCCC TCTCACTGTC CCTAAGCTTG 
GCCCCAAACA GAGCTGTCCA TGAGGTGAAG GACAGGAATG AAGCTAGCAG CCTTATTCAT 
AAAGCACTCA GCAGAGCCAC AGAGAAACTT CCGACAAACC CCCATCATCT TACAGCCCTG 
TGCTGCAGAG TGTTCTACAG GAGGGATGGC ACAGAGTCAC AGAGGCTACA GGAAGGGCTT 
CAGGGAGTTC TGGGAGAAGT CTGGCCTGAG AAACCTCCAG CTCTGGTACT GGTGCCTGTA 
ATGGACCTGC CTTGTAACCA GATCCTACAC TTGTCCTTCT GGCTTAGTCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dph6 ( XM_031891250.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031747110.1 Xenopus tropicalis diphthamine biosynthesis 6 (dph6), transcript variant X3, mRNA.

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CloneID	OXa64499
Clone ID Related Accession (Same CDS sequence)	XM_031891250.1
Accession Version	XM_031891250.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2025bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	diphthine--ammonia ligase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCAGT GTGTTTCTGC TGGCCACCAG ATTGTTGCCC TGGCAAACCT GAAGCCTCCA 
GAAAGCACAG CTGATGAGTT AGACAGTTAC ATGTACCAGA CTGTTGGGCA TCATGCGTTA 
GAGCTGTATG CAGAAGCCAT GGGACTGCCT ATCTATCGTG CCACTCTGCA GGGCACCAGC 
TTGGATACAG GCAGAGCTTA TGCCCCACAG GAAGGGGACG AGGTGGAGGA TCTATATCGA 
CTACTGAAGC TGGTGAAGGA AAAGGAGGCT GTGGATTCTG TTTCTGTTGG GGCAATCCTG 
TCTGATTACC AGCGTGTCCG CGTGGAGAAT GTGTGTCAGA GGCTCGGCCT CCAGCCTTTA 
GCTTTCCTGT GGCGTAGGAA GCAAGAAGAA CTTCTGGATG AAATGATCTC CTCGGGTCTG 
AAGGCCATCC TTATCAAGGT AGCTGCTTTT GGGTTAGATC CAGACAAGCA CCTGGGGAAG 
ACACTGGAAG ACATGAGGCC TTACCTTAAG CAGCTCTCTG ACAAGTATGG TGTCCATGTT 
TGCGGAGAAG GAGGAGAGTA TGAGACGCTC ACTTTAGACT GTCCTCTATT CAAGAAAAGA 
ATTGTTGTGG ATTCTGCGGA GGTGACGATG CATTCTAATG ATGCCTTCGC TCCTGTGGCT 
TATCTTCGAT TATCGAAGCT TCACCTTGAG GATAAAATTA CCAACACAGC AGTTACATTG 
CAAAGAGAGT GTCCCTGTAG TAATGGATTC CCTGCAGAGC CTGCATCCTG TGCCACAAAG 
GAATGTGATA ATTCTGAGGA ACGGCCATTG CCCTGGATAC CGAACAGTCT CCCTTCGGTG 
CCCATGTTCT GTTTGCCTGT AGGTGAATCA AGAACTTGTT CAGCTCCATC ACAATGTGGA 
TTCCAGTGGA TCAGTGAGAT TACTGCCAGT GAGGGAAATG TCTTGGAGGC TTCCCACAGG 
GCACTCTCTT CACTTAAAGA CCAAGTACAA GAGTTGGGCC TGCAGATGAG TGATGCTGTG 
CTTATACATC TTTATGTCAA GAGCATGGAG GACTTTTCTG ATATAAATAG GGTCTATGGG 
ACACTCTTCC CATCTGCTCC CCCAGCTCGG GTTTGTGTGC AGTGTTGTCT TCCCGGCAGT 
GCATTTTTCA AGATGGATGC CCTATTCTGC TGTCCTTCAC TTGCAGACGC TAGCAGTGAC 
TGTGTGCCGA AGAAGACGGC AATGCATGTG CAGAGCATCT CTCACTGGGC CCCTGCTAAC 
ATTGGGCCCT ACAGCCAGGC TGTGCAGGTT GGTCCTGGTT TATTCTGTGC TGGACAAATT 
GCACTGAAAC CCTGTACCAT GCAGCTGGTA TCTGGGGGAG CTACAGTGGA AGCCCAGGTC 
TCCCTGTGCC ATGTGGAACG TGTCCTGGAC GCCATTAACC TTGGTACCTC CTTGGCCCAC 
GTGCTGCTTG TTCAATGCTA TGTAACAAAT CGCTCCTACA TACCCATCGC CTTAGCTGCT 
TGGGGTAGCA GAAATGCACA AGAGGCTGAT ATTGTCCCTC TTACCGTGGT GGTAGTACCA 
ATGCTTCCCC GTGGTGCTTC AGTTGAGTGG CATGTTATAG CTGCCGTGAG CGACCCAGCA 
GAAAGACAAC ACTTCTCAGT ATGCCAGGAA AATCTGGGCT ACCAGGTGAT GCTTTATGGG 
GTGATATCCA GTTGTGTGTC CTGTGCCTCC CTCTCACTGT CCCTAAGCTT GGCCCCAAAC 
AGAGCTGTCC ATGAGGTGAA GGACAGGAAT GAAGCTAGCA GCCTTATTCA TAAAGCACTC 
AGCAGAGCCA CAGAGAAACT TCCGACAAAC CCCCATCATC TTACAGCCCT GTGCTGCAGA 
GTGTTCTACA GGAGGGATGG CACAGAGTCA CAGAGGCTAC AGGAAGGGCT TCAGGGAGTT 
CTGGGAGAAG TCTGGCCTGA GAAACCTCCA GCTCTGGTAC TGGTGCCTGT AATGGACCTG 
CCTTGTAACC AGATCCTACA CTTGTCCTTC TGGCTTAGTC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031747110.1
CDS	48..2072
Translation	MMQCVSAGHQIVALANLKPPESTADELDSYMYQTVGHHALELYAEAMGLPIYRATLQGTSLDTGRAYAPQEGDEVEDLYRLLKLVKEKEAVDSVSVGAILSDYQRVRVENVCQRLGLQPLAFLWRRKQEELLDEMISSGLKAILIKVAAFGLDPDKHLGKTLEDMRPYLKQLSDKYGVHVCGEGGEYETLTLDCPLFKKRIVVDSAEVTMHSNDAFAPVAYLRLSKLHLEDKITNTAVTLQRECPCSNGFPAEPASCATKECDNSEERPLPWIPNSLPSVPMFCLPVGESRTCSAPSQCGFQWISEITASEGNVLEASHRALSSLKDQVQELGLQMSDAVLIHLYVKSMEDFSDINRVYGTLFPSAPPARVCVQCCLPGSAFFKMDALFCCPSLADASSDCVPKKTAMHVQSISHWAPANIGPYSQAVQVGPGLFCAGQIALKPCTMQLVSGGATVEAQVSLCHVERVLDAINLGTSLAHVLLVQCYVTNRSYIPIALAAWGSRNAQEADIVPLTVVVVPMLPRGASVEWHVIAAVSDPAERQHFSVCQENLGYQVMLYGVISSCVSCASLSLSLSLAPNRAVHEVKDRNEASSLIHKALSRATEKLPTNPHHLTALCCRVFYRRDGTESQRLQEGLQGVLGEVWPEKPPALVLVPVMDLPCNQILHLSFWLSL

Target ORF information:

RefSeq Version	XM_031891250.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diphthamine biosynthesis 6 (dph6), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891250.1

ORF Insert Sequence:

ATGATGCAGT GTGTTTCTGC TGGCCACCAG ATTGTTGCCC TGGCAAACCT GAAGCCTCCA 
GAAAGCACAG CTGATGAGTT AGACAGTTAC ATGTACCAGA CTGTTGGGCA TCATGCGTTA 
GAGCTGTATG CAGAAGCCAT GGGACTGCCT ATCTATCGTG CCACTCTGCA GGGCACCAGC 
TTGGATACAG GCAGAGCTTA TGCCCCACAG GAAGGGGACG AGGTGGAGGA TCTATATCGA 
CTACTGAAGC TGGTGAAGGA AAAGGAGGCT GTGGATTCTG TTTCTGTTGG GGCAATCCTG 
TCTGATTACC AGCGTGTCCG CGTGGAGAAT GTGTGTCAGA GGCTCGGCCT CCAGCCTTTA 
GCTTTCCTGT GGCGTAGGAA GCAAGAAGAA CTTCTGGATG AAATGATCTC CTCGGGTCTG 
AAGGCCATCC TTATCAAGGT AGCTGCTTTT GGGTTAGATC CAGACAAGCA CCTGGGGAAG 
ACACTGGAAG ACATGAGGCC TTACCTTAAG CAGCTCTCTG ACAAGTATGG TGTCCATGTT 
TGCGGAGAAG GAGGAGAGTA TGAGACGCTC ACTTTAGACT GTCCTCTATT CAAGAAAAGA 
ATTGTTGTGG ATTCTGCGGA GGTGACGATG CATTCTAATG ATGCCTTCGC TCCTGTGGCT 
TATCTTCGAT TATCGAAGCT TCACCTTGAG GATAAAATTA CCAACACAGC AGTTACATTG 
CAAAGAGAGT GTCCCTGTAG TAATGGATTC CCTGCAGAGC CTGCATCCTG TGCCACAAAG 
GAATGTGATA ATTCTGAGGA ACGGCCATTG CCCTGGATAC CGAACAGTCT CCCTTCGGTG 
CCCATGTTCT GTTTGCCTGT AGGTGAATCA AGAACTTGTT CAGCTCCATC ACAATGTGGA 
TTCCAGTGGA TCAGTGAGAT TACTGCCAGT GAGGGAAATG TCTTGGAGGC TTCCCACAGG 
GCACTCTCTT CACTTAAAGA CCAAGTACAA GAGTTGGGCC TGCAGATGAG TGATGCTGTG 
CTTATACATC TTTATGTCAA GAGCATGGAG GACTTTTCTG ATATAAATAG GGTCTATGGG 
ACACTCTTCC CATCTGCTCC CCCAGCTCGG GTTTGTGTGC AGTGTTGTCT TCCCGGCAGT 
GCATTTTTCA AGATGGATGC CCTATTCTGC TGTCCTTCAC TTGCAGACGC TAGCAGTGAC 
TGTGTGCCGA AGAAGACGGC AATGCATGTG CAGAGCATCT CTCACTGGGC CCCTGCTAAC 
ATTGGGCCCT ACAGCCAGGC TGTGCAGGTT GGTCCTGGTT TATTCTGTGC TGGACAAATT 
GCACTGAAAC CCTGTACCAT GCAGCTGGTA TCTGGGGGAG CTACAGTGGA AGCCCAGGTC 
TCCCTGTGCC ATGTGGAACG TGTCCTGGAC GCCATTAACC TTGGTACCTC CTTGGCCCAC 
GTGCTGCTTG TTCAATGCTA TGTAACAAAT CGCTCCTACA TACCCATCGC CTTAGCTGCT 
TGGGGTAGCA GAAATGCACA AGAGGCTGAT ATTGTCCCTC TTACCGTGGT GGTAGTACCA 
ATGCTTCCCC GTGGTGCTTC AGTTGAGTGG CATGTTATAG CTGCCGTGAG CGACCCAGCA 
GAAAGACAAC ACTTCTCAGT ATGCCAGGAA AATCTGGGCT ACCAGGTGAT GCTTTATGGG 
GTGATATCCA GTTGTGTGTC CTGTGCCTCC CTCTCACTGT CCCTAAGCTT GGCCCCAAAC 
AGAGCTGTCC ATGAGGTGAA GGACAGGAAT GAAGCTAGCA GCCTTATTCA TAAAGCACTC 
AGCAGAGCCA CAGAGAAACT TCCGACAAAC CCCCATCATC TTACAGCCCT GTGCTGCAGA 
GTGTTCTACA GGAGGGATGG CACAGAGTCA CAGAGGCTAC AGGAAGGGCT TCAGGGAGTT 
CTGGGAGAAG TCTGGCCTGA GAAACCTCCA GCTCTGGTAC TGGTGCCTGT AATGGACCTG 
CCTTGTAACC AGATCCTACA CTTGTCCTTC TGGCTTAGTC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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dph6 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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