myh3 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	myh3
Entrez Gene ID	448762
Full Name	myosin, heavy chain 3, skeletal muscle, embryonic
Synonyms	LM,myh2
General protein information	Preferred Names myosin, heavy chain 3, skeletal muscle, embryonic Names myosin heavy chain IIa myosin, heavy polypeptide 2, skeletal muscle, adult
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001006914.1	NP_001006915.1	myosin heavy chain IIa
XM_012954178.3	XP_012809632.1	myosin heavy chain IIa isoform X1
XM_012954178.2	XP_012809632.1	myosin heavy chain IIa isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MYH4	XP_523785.2
	MYH4	XP_002800337.1
Canis lupus familiaris (dog)	MYH4	NP_001070262.1
Danio rerio (zebrafish)	myhz2	NP_694514.2
Danio rerio (zebrafish)	myhz1.2	NP_001154918.1
Danio rerio (zebrafish)	myhb	XP_001339206.5
Xenopus tropicalis (tropical clawed frog)	myh3	NP_001006915.1
Homo sapiens (human)	MYH4	NP_060003.2
Rattus norvegicus (Norway rat)	Myh4	NP_062198.1
Danio rerio (zebrafish)	ns:zf-e68	XP_001924014.2
Danio rerio (zebrafish)	zgc:66156	XP_002666141.3
Bos taurus (cattle)	MYH4	XP_005220494.1
Mus musculus (house mouse)	Myh4	NP_034985.2
Danio rerio (zebrafish)	myhc4	NP_001018321.1
Danio rerio (zebrafish)	wu:fd14a01	XP_002667379.3
Danio rerio (zebrafish)	myhz1.1	NP_001108561.1
Danio rerio (zebrafish)	myha	XP_001924009.1
Xenopus tropicalis (tropical clawed frog)	LOC100492956	XP_002937039.1
Xenopus tropicalis (tropical clawed frog)	myh7	NP_001135564.1
Xenopus tropicalis (tropical clawed frog)	LOC100493121	XP_004919657.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following myh3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the myh3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa23509	XM_012954178.3 Latest version!	Xenopus tropicalis myosin, heavy chain 3, skeletal muscle, embryonic (myh3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa23509	NM_001006914.1 Latest version!	Xenopus tropicalis myosin, heavy chain 3, skeletal muscle, embryonic (myh3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa26781	XM_012954178.2 Latest version!	Xenopus tropicalis myosin, heavy chain 3, skeletal muscle, embryonic (myh3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

myh3 ( XM_012954178.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012809632.1 Xenopus tropicalis myosin, heavy chain 3, skeletal muscle, embryonic (myh3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa23509
Clone ID Related Accession (Same CDS sequence)	NM_001006914.1 , XM_012954178.3
Accession Version	XM_012954178.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	myosin heavy chain IIa isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012954178.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCGG ACGCGGAGAT GGCCGTTTTC GGGCCAGCAG CCCATTTTCT CAGAAAAAGT 
GAGAAAGAGC GGATCGAGGC GCAGAACAAA CCATTCGACG CAAGGACCTC CATGTTTGTG 
GTGGATACCA AGGAATCCTA CGTGAAAGGG ACCCTCCAAA GCAAGGAGGG CGGGAAGGCT 
ACTGTAAAGA CAGAAGCGGG ACAGACTGTA ACAGTGAAGG ATGATCAGAT CTTCCCCATG 
AACCCACCAA AGTTCGATAA AATTGAAGAC ATGGCCATGA TGACCCACTT GAACGAACCA 
TCTGTCCTGT ATAACCTCAA AGAGCGTTAC GCAGCCTGGA TGATCTACAC CTATTCTGGG 
CTCTTCTGCG TCACTGTCAA CCCCTACAAG TGGCTTCCCG TGTACAACCC AGAGGTGGTC 
AATGCCTACC GTGGGAAGAA GCGTCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAT 
AACGCCTATC AGTTCATGTT AACTGATCGT GAGAACCAGT CTATCCTGAT CACTGGAGAA 
TCCGGTGCCG GGAAGACCGT AAACACCAAG CGTGTCATTC AGTATTTTGC AACAATTGCA 
GCCGCCCCCG ACAAGAAAAA AGAAGAGGTG GCAAAGACCA AGGGGACCCT GGAGGATCAA 
ATCATTCAGG CCAACCCGCT GCTGGAAGCC TTCGGCAATG CCAAGACCGT CAGGAACGAC 
AACTCTTCCC GCTTTGGTAA ATTCATCAGA ATCCATTTCG GCACGACTGG GAAACTCGCC 
TCTGCTGATA TTGAAACATA CCTGCTGGAG AAGTCCAGAG TTACCTTCCA GCTAAAGGCC 
GAGAGGAGCT ATCACATTTT CTACCAAATC ATGTCCAACA AAAAGCCCGA ACTGATTGAA 
ATGCTCCTCA TCACCACCAA CCCCTACGAC TTTCCATTCG TCAGCCAAGG GGAGATCTCT 
GTGGCCAGTA TCGACGATAC GGAGGAACTC ATGGCCACTG ATAGCGCCAT CGATATCCTG 
GGCTTCAATG CGGATGAGAA AGTTTCTATC TATAAACTGA CCGGTGCCGT CATGCACTAC 
GGGAACATGA GATTCAAGCA GAAACAGCGA GAGGAGCAGG CCGAGCCGGA CGGCACAGAA 
GTGGCCGACA AAGCTGCCTA CTTGATGTGC CTGAACTCAG CAGATCTGCT GAAGGCTCTG 
TGCTACCCAA GGGTCAAGGT CGGCAATGAA TATGTCACCA AGGGGCAAAC TGTCCAGCAG 
GTGTACAACT CAGTCGGCGC TCTGGGCAAA GCTGTTTACG AGAAGATGTT CTTGTGGATG 
GTCACGCGTA TCAATGAAAT GTTGGACACC AAGCAGCCAA GGCAGTATTT CATCGGTGTG 
CTGGACATCG CTGGCTTTGA GATCTTTGAT TTCAACAGCT TGGAGCAGCT CTGCATCAAC 
TTCACCAACG AGAAACTGCA ACAGTTTTTC AACCACCACA TGTTTGTCCT GGAGCAAGAG 
GAGTACAAGA AGGAGGGGAT CGAGTGGGAG TTCATTGACT TCGGCATGGA CTTGGCCGCC 
TGCATTGAGC TTATTGAGAA GCCAATGGGC ATTTTCTCCA TCCTTGAAGA GGAGTGCATG 
TTCCCCAAGG CAACAGACAC TTCATTCAAG AACAAGTTGT ACGACCAACA TCTGGGCAAG 
TCCAACAACT TCCAGAAGCC CAAACCGGCC AAAGGCAAAG CCGAGGCCCA CTTCTCTCTT 
GTCCACTATG CCGGGACGGT AGATTATAAC ATCAGTGGTT GGCTTGAAAA GAACAAGGAC 
CCACTGAATG ACACTGTCAT TGGCCTCTTC CAGAAGTCTT CAATGAAGAC ACTGGCGTAT 
CTGTTCTCCA GCTATGCTGC TTCTGAAGCA GAGGGTGCAA AGAAAGGTGG TAAGAAGAAG 
GGGTCTTCTT TCCAGACCGT GTCTGCTCTT TTTAGGGAAA ATCTGAATAA GCTAATGAGC 
AATCTAAGGA CCACCCATCC TCACTTTGTC CGTTGCATTA TTCCCAATGA GACCAAAACT 
CCAGGGGCAA TGGAACATGA ACTTGTATTG CATCAGCTCA GGTGTAATGG GGTGCTGGAG 
GGGATCAGGA TTTGCAGAAA GGGATTCCCA AGCAGGATTC TCTACGGTGA CTTCAAGCAA 
AGATACAAAA TTTTAAATGC AAGTGCCATC CCAGAGGGGC AGTTTATTGA CAGCAAGAAG 
GCCTCTGAAA AACTTCTTGG ATCCATTGAT GTTGATCATA CCCAGTACAG ATTTGGCCAC 
ACTAAGGTGT TCTTCAAAGC TGGTCTTCTG GGTACCCTGG AAGAGATGCG AGACGATCGC 
TTGGCCCATG TAATCACACG TACCCAGGCT ATGTGCAGAG GGTACCTCAT GAGGGTGGAG 
TTTAAGAAAA TGATGGAGAG AAGGGAAGCT GTGTTCACTA TTCAATACAA TATCCGGTCC 
TTCATGAATG TCAAGCATTG GCCATGGATG AAGCTTTATT TCAAGATCAA GCCCCTCCTG 
AAGAGTGCCG AGTCTGAGAA AGAGATGCAG AACATGAAGG AAGAGTTTGA GAAGACCAAG 
GAGCTGCTGG CCAAGTCAGA AGCTAAACGT AAGGAGCTGG AAGAGAAGAT GGTTAAAGTT 
CTCCAAGAGA AGAATGACCT GCAACTTCAA GTCCAGTCTG AATCAGAAAA TCTCACAGAT 
GCCGAGGAAA GGTGCGATCA GTTAATCAAA TCCAAAATCC AGTTAGAAGC CAAAATGAAA 
GAGCTGAATG AGAGGCTGGA AGACGAGGAG GAATCCAACG CTGAACTGAC AGCCAAGAAG 
AGGAAACTTG AAGATGAATG CTCAGAACTG AAGAAAGATA TTGACGATCT GGAGTTAACG 
CTGGCCAAAG TTGAAAAAGA GAAACACGCA ACAGAGAACA AGGTTAAGAA CCTCACTGAA 
GAAATGGCAG TTTTGGATGA GACCATCGTT AAGCTTACCA AAGAGAAAAA AGCCCTTCAA 
GAAGCTCACC AGCAGACCCT TGATGACCTC CAGGCGGAGG AAGACAAAGT CAACACTCTG 
ACCAAAGCCA AGGCCAAGCT TGAGCAGCAA GTCGACGATC TTGAAGGGTC TTTGGAACAA 
GAGAAGAAAC TGCGCATGGA CCTTGAAAGA TCTAAGAGAA AGCTAGAGGG TGATCTTAAA 
CTTGCCCAGG AAACCATCAT GGACCTGGAA AACGATAGGC AGCAACTGGA TGAAAAGCTA 
AAAAAGAAAG AATTTGAAAT GAGCCAGTAC GCAAGCAAAA TTGAGGATGA GCAGGCTCTT 
GGTGCTCAAC TGCAGAAAAA GATTAAGGAA TTGCAGGCCC GCATTGAGGA GCTAGAAGAA 
GAAATAGAAG CTGAGCGTGC AGCTCGTGCC AAGGCAGAGA AGCAGCGTTC CGACCTCTCC 
AGGGAACTTG AGGAGATCAG TGAGCGCCTG GAGGAAGCTG GAGGTGCCAC CTCTGCTCAG 
ATTGAGATGA ACAAGAAGCG CGAGGCAGAA TTCCAGAAGA TGAGACGTGA CCTTGAAGAG 
GCCACTCTGC AACACGAAGC CACCGCTGCT GCTCTCAGAA AGAAGCAGGC CGACAGCGTG 
GCTGAACTCG GGGAACAAAT CGATAACCTG CAAAGAGTCA AGCAGAAGTT AGAAAAGGAG 
AAAAGTGAGC TAAAGATGGA GATTGATGAC CTGGCCAGCA ACATGGAAAA CCTATCCAAA 
GCCAAGGCCA ATTTTGAGAA GATTTGCCGC ACCCTTGAAG ATCAGCTGAG TGAACTCAAG 
ACAAAGGATG AGGAACACCA GAGGCAGATT AACGACCTCA CTGCCCAAAG GGCCCGACTG 
CAGACAGAAA ATGGTGAATA TGCACGACAG TTGGAGGAAA GAGATTCTTT GATCTCCCAG 
CTTTCTCGAG GCAAACAGGC ATTTACTCAA CAGATTGAAG AGCTCAAGAG GCAGCTGGAA 
GAGGAAATCA AGGCCAAGAA TGCCCTTGCC CATGGTCTGC AGTCAGCCCG CCATGATTGC 
GACTTGCTTA GAGAACAATA TGAGGAAGAG CAAGAGGCCA AGGGTGAGCT TCAGCGCTCG 
TTGTCCAAAG CCAATGGTGA AGTTGCCCAG TGGAGGACAA AGTACGAGAC AGACGCTATT 
CAGCGTACAG AAGAACTAGA GGATGCCAAG AAGAAACTGG CTCAGCGTCT CCAGGAAGCA 
GAAGAACACA TTGAAGCTGT GAACTCAAAG TGCGCTTCTC TCGAAAAGAC CAAACAGCGA 
CTTCAGAATG AAGTTGAAGA CCTTATGGTT GATGTAGAAC GGTCAAACAG TGCTTGTGCT 
GCTCTAGATA AGAAACAGAA GAACTTCGAT AAGATTCTGG CAGAATGGAA GCAGAAGTTT 
GAAGAGAGCC AGGCTGAACT GGAAGCCTCT CAGAAGGAGT CCCGGTCTCT CAGCACAGAG 
TTGTTCAAGA TGAAGAATGC CTATGAGGAA TCCCTGGATC ACCTCGAGAC AACCAAACGA 
GAGAACAAGA ACTTGCAGCA GGAGATTTCC GATCTTACTG AACAAGTTTC TGAAGTTTCC 
AAGACACTGC ACGAGGTGGA AAAAGCCAAG AAAATTATTG AGCAAGAAAA GACTGAAATT 
CAGTCTGCTC TAGAAGAAGC TGAGGCATCT CTGGAACACG AGGAAGGCAA GATACTGAGA 
ATCCAGCTGG AACTGAATCA AGTGAAATCA GAAATTGACA GAAAGATTGC AGAGAAGGAT 
GAGGAAATCG ATCAGTTGAA AAGGAACCAC GTCAGGGTGG TTGAGTCCAT GCAGAGCACA 
CTGGATGCTG AAATCAGAAG CAGGAACGAT GCTCTAAGGT TGAAGAAGAA AATGGAAGGA 
GATCTCAATG AAATGGAGAT CCAGTTGAGC CATGCCAATC GACAAGCTTC TGAGTCTCAG 
AAGCAGCTGA GAAATGTCCA GGGATTGCTC AAGGATGTTC AGATCCACTT GGACGACTCC 
CTGAGAGCCC AGGAAGATCT TAAGGAGCAA TTGGCTATGG TTGAACGCAG AGCTAATCTG 
ATGCAGGCTG AGATAGAAGA GATGAGGGCT GCTCTGGAAC AAACAGAACG GAGCAGGAAA 
GTCGCAGAAC AAGAACTACT GGATGCTAGT GAGCGTGTCC AGCTCCTGCA CTCACAGAAC 
ACAAGCTTGA TCAACACCAA GAAGAAGATG GAGACAGACA TCACTCAGCT ACAGACTGAG 
GTTGAAGAGG CCATACAAGA AGCCCGCAAT GCAGAAGAAA AGGCCAAAAA GGCCATAACA 
GATGCTGCCA TGATGGCAGA AGAGCTTAAA AAGGAACAGG ACACCAGTGC TCACTTGGAA 
AGGATGAAGA AGAACCTCGA TCAAACAGTG AAGGATCTGC AGCACCGTTT GGATGAAGCT 
GAACAACTGG CAATGAAAGG AGGAAAGAAA CAAATCCAGA AACTGGAGGC TAGGGTTCGG 
GAGCTTGAAA ATGAAGTGGA TGTAGAACAG AAACGCAGCA CAGAGGCCAT TAAAGGCGTC 
CGAAAATACG AACGGCGGGT TAAAGAACTA TCATACCAGT CTGAGGAGGA TAAGAAGAAT 
ATCCTGAGAC TTCAAGACCT GGTGGACAAA CTCCAAATGA AGGTGAAGGC CTACAAGAGG 
CAGACAGAGG AAATTGAGGA ACAGTCCAAT ATCAACCTCA CCAAATACCG GAAGATCCAA 
CATGAACTTG AGGAAGCCGA GGAACGTGCC GATATTGCAG AATCCCAGGT GAACAAGCTC 
CGCGCCAAGA CCCGAGAGGT CACCAAGATC ACGAAGAGCG AAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012809632.1
CDS	108..5915
Translation	MSSDAEMAVFGPAAHFLRKSEKERIEAQNKPFDARTSMFVVDTKESYVKGTLQSKEGGKATVKTEAGQTVTVKDDQIFPMNPPKFDKIEDMAMMTHLNEPSVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPEVVNAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAAAPDKKKEEVAKTKGTLEDQIIQANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQIMSNKKPELIEMLLITTNPYDFPFVSQGEISVASIDDTEELMATDSAIDILGFNADEKVSIYKLTGAVMHYGNMRFKQKQREEQAEPDGTEVADKAAYLMCLNSADLLKALCYPRVKVGNEYVTKGQTVQQVYNSVGALGKAVYEKMFLWMVTRINEMLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDQHLGKSNNFQKPKPAKGKAEAHFSLVHYAGTVDYNISGWLEKNKDPLNDTVIGLFQKSSMKTLAYLFSSYAASEAEGAKKGGKKKGSSFQTVSALFRENLNKLMSNLRTTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYGDFKQRYKILNASAIPEGQFIDSKKASEKLLGSIDVDHTQYRFGHTKVFFKAGLLGTLEEMRDDRLAHVITRTQAMCRGYLMRVEFKKMMERREAVFTIQYNIRSFMNVKHWPWMKLYFKIKPLLKSAESEKEMQNMKEEFEKTKELLAKSEAKRKELEEKMVKVLQEKNDLQLQVQSESENLTDAEERCDQLIKSKIQLEAKMKELNERLEDEEESNAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAVLDETIVKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKAKLEQQVDDLEGSLEQEKKLRMDLERSKRKLEGDLKLAQETIMDLENDRQQLDEKLKKKEFEMSQYASKIEDEQALGAQLQKKIKELQARIEELEEEIEAERAARAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKQADSVAELGEQIDNLQRVKQKLEKEKSELKMEIDDLASNMENLSKAKANFEKICRTLEDQLSELKTKDEEHQRQINDLTAQRARLQTENGEYARQLEERDSLISQLSRGKQAFTQQIEELKRQLEEEIKAKNALAHGLQSARHDCDLLREQYEEEQEAKGELQRSLSKANGEVAQWRTKYETDAIQRTEELEDAKKKLAQRLQEAEEHIEAVNSKCASLEKTKQRLQNEVEDLMVDVERSNSACAALDKKQKNFDKILAEWKQKFEESQAELEASQKESRSLSTELFKMKNAYEESLDHLETTKRENKNLQQEISDLTEQVSEVSKTLHEVEKAKKIIEQEKTEIQSALEEAEASLEHEEGKILRIQLELNQVKSEIDRKIAEKDEEIDQLKRNHVRVVESMQSTLDAEIRSRNDALRLKKKMEGDLNEMEIQLSHANRQASESQKQLRNVQGLLKDVQIHLDDSLRAQEDLKEQLAMVERRANLMQAEIEEMRAALEQTERSRKVAEQELLDASERVQLLHSQNTSLINTKKKMETDITQLQTEVEEAIQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLDQTVKDLQHRLDEAEQLAMKGGKKQIQKLEARVRELENEVDVEQKRSTEAIKGVRKYERRVKELSYQSEEDKKNILRLQDLVDKLQMKVKAYKRQTEEIEEQSNINLTKYRKIQHELEEAEERADIAESQVNKLRAKTREVTKITKSEE

Target ORF information:

RefSeq Version	XM_012954178.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin, heavy chain 3, skeletal muscle, embryonic (myh3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012954178.3

ORF Insert Sequence:

ATGAGTTCGG ACGCGGAGAT GGCCGTTTTC GGGCCAGCAG CCCATTTTCT CAGAAAAAGT 
GAGAAAGAGC GGATCGAGGC GCAGAACAAA CCATTCGACG CAAGGACCTC CATGTTTGTG 
GTGGATACCA AGGAATCCTA CGTGAAAGGG ACCCTCCAAA GCAAGGAGGG CGGGAAGGCT 
ACTGTAAAGA CAGAAGCGGG ACAGACTGTA ACAGTGAAGG ATGATCAGAT CTTCCCCATG 
AACCCACCAA AGTTCGATAA AATTGAAGAC ATGGCCATGA TGACCCACTT GAACGAACCA 
TCTGTCCTGT ATAACCTCAA AGAGCGTTAC GCAGCCTGGA TGATCTACAC CTATTCTGGG 
CTCTTCTGCG TCACTGTCAA CCCCTACAAG TGGCTTCCCG TGTACAACCC AGAGGTGGTC 
AATGCCTACC GTGGGAAGAA GCGTCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAT 
AACGCCTATC AGTTCATGTT AACTGATCGT GAGAACCAGT CTATCCTGAT CACTGGAGAA 
TCCGGTGCCG GGAAGACCGT AAACACCAAG CGTGTCATTC AGTATTTTGC AACAATTGCA 
GCCGCCCCCG ACAAGAAAAA AGAAGAGGTG GCAAAGACCA AGGGGACCCT GGAGGATCAA 
ATCATTCAGG CCAACCCGCT GCTGGAAGCC TTCGGCAATG CCAAGACCGT CAGGAACGAC 
AACTCTTCCC GCTTTGGTAA ATTCATCAGA ATCCATTTCG GCACGACTGG GAAACTCGCC 
TCTGCTGATA TTGAAACATA CCTGCTGGAG AAGTCCAGAG TTACCTTCCA GCTAAAGGCC 
GAGAGGAGCT ATCACATTTT CTACCAAATC ATGTCCAACA AAAAGCCCGA ACTGATTGAA 
ATGCTCCTCA TCACCACCAA CCCCTACGAC TTTCCATTCG TCAGCCAAGG GGAGATCTCT 
GTGGCCAGTA TCGACGATAC GGAGGAACTC ATGGCCACTG ATAGCGCCAT CGATATCCTG 
GGCTTCAATG CGGATGAGAA AGTTTCTATC TATAAACTGA CCGGTGCCGT CATGCACTAC 
GGGAACATGA GATTCAAGCA GAAACAGCGA GAGGAGCAGG CCGAGCCGGA CGGCACAGAA 
GTGGCCGACA AAGCTGCCTA CTTGATGTGC CTGAACTCAG CAGATCTGCT GAAGGCTCTG 
TGCTACCCAA GGGTCAAGGT CGGCAATGAA TATGTCACCA AGGGGCAAAC TGTCCAGCAG 
GTGTACAACT CAGTCGGCGC TCTGGGCAAA GCTGTTTACG AGAAGATGTT CTTGTGGATG 
GTCACGCGTA TCAATGAAAT GTTGGACACC AAGCAGCCAA GGCAGTATTT CATCGGTGTG 
CTGGACATCG CTGGCTTTGA GATCTTTGAT TTCAACAGCT TGGAGCAGCT CTGCATCAAC 
TTCACCAACG AGAAACTGCA ACAGTTTTTC AACCACCACA TGTTTGTCCT GGAGCAAGAG 
GAGTACAAGA AGGAGGGGAT CGAGTGGGAG TTCATTGACT TCGGCATGGA CTTGGCCGCC 
TGCATTGAGC TTATTGAGAA GCCAATGGGC ATTTTCTCCA TCCTTGAAGA GGAGTGCATG 
TTCCCCAAGG CAACAGACAC TTCATTCAAG AACAAGTTGT ACGACCAACA TCTGGGCAAG 
TCCAACAACT TCCAGAAGCC CAAACCGGCC AAAGGCAAAG CCGAGGCCCA CTTCTCTCTT 
GTCCACTATG CCGGGACGGT AGATTATAAC ATCAGTGGTT GGCTTGAAAA GAACAAGGAC 
CCACTGAATG ACACTGTCAT TGGCCTCTTC CAGAAGTCTT CAATGAAGAC ACTGGCGTAT 
CTGTTCTCCA GCTATGCTGC TTCTGAAGCA GAGGGTGCAA AGAAAGGTGG TAAGAAGAAG 
GGGTCTTCTT TCCAGACCGT GTCTGCTCTT TTTAGGGAAA ATCTGAATAA GCTAATGAGC 
AATCTAAGGA CCACCCATCC TCACTTTGTC CGTTGCATTA TTCCCAATGA GACCAAAACT 
CCAGGGGCAA TGGAACATGA ACTTGTATTG CATCAGCTCA GGTGTAATGG GGTGCTGGAG 
GGGATCAGGA TTTGCAGAAA GGGATTCCCA AGCAGGATTC TCTACGGTGA CTTCAAGCAA 
AGATACAAAA TTTTAAATGC AAGTGCCATC CCAGAGGGGC AGTTTATTGA CAGCAAGAAG 
GCCTCTGAAA AACTTCTTGG ATCCATTGAT GTTGATCATA CCCAGTACAG ATTTGGCCAC 
ACTAAGGTGT TCTTCAAAGC TGGTCTTCTG GGTACCCTGG AAGAGATGCG AGACGATCGC 
TTGGCCCATG TAATCACACG TACCCAGGCT ATGTGCAGAG GGTACCTCAT GAGGGTGGAG 
TTTAAGAAAA TGATGGAGAG AAGGGAAGCT GTGTTCACTA TTCAATACAA TATCCGGTCC 
TTCATGAATG TCAAGCATTG GCCATGGATG AAGCTTTATT TCAAGATCAA GCCCCTCCTG 
AAGAGTGCCG AGTCTGAGAA AGAGATGCAG AACATGAAGG AAGAGTTTGA GAAGACCAAG 
GAGCTGCTGG CCAAGTCAGA AGCTAAACGT AAGGAGCTGG AAGAGAAGAT GGTTAAAGTT 
CTCCAAGAGA AGAATGACCT GCAACTTCAA GTCCAGTCTG AATCAGAAAA TCTCACAGAT 
GCCGAGGAAA GGTGCGATCA GTTAATCAAA TCCAAAATCC AGTTAGAAGC CAAAATGAAA 
GAGCTGAATG AGAGGCTGGA AGACGAGGAG GAATCCAACG CTGAACTGAC AGCCAAGAAG 
AGGAAACTTG AAGATGAATG CTCAGAACTG AAGAAAGATA TTGACGATCT GGAGTTAACG 
CTGGCCAAAG TTGAAAAAGA GAAACACGCA ACAGAGAACA AGGTTAAGAA CCTCACTGAA 
GAAATGGCAG TTTTGGATGA GACCATCGTT AAGCTTACCA AAGAGAAAAA AGCCCTTCAA 
GAAGCTCACC AGCAGACCCT TGATGACCTC CAGGCGGAGG AAGACAAAGT CAACACTCTG 
ACCAAAGCCA AGGCCAAGCT TGAGCAGCAA GTCGACGATC TTGAAGGGTC TTTGGAACAA 
GAGAAGAAAC TGCGCATGGA CCTTGAAAGA TCTAAGAGAA AGCTAGAGGG TGATCTTAAA 
CTTGCCCAGG AAACCATCAT GGACCTGGAA AACGATAGGC AGCAACTGGA TGAAAAGCTA 
AAAAAGAAAG AATTTGAAAT GAGCCAGTAC GCAAGCAAAA TTGAGGATGA GCAGGCTCTT 
GGTGCTCAAC TGCAGAAAAA GATTAAGGAA TTGCAGGCCC GCATTGAGGA GCTAGAAGAA 
GAAATAGAAG CTGAGCGTGC AGCTCGTGCC AAGGCAGAGA AGCAGCGTTC CGACCTCTCC 
AGGGAACTTG AGGAGATCAG TGAGCGCCTG GAGGAAGCTG GAGGTGCCAC CTCTGCTCAG 
ATTGAGATGA ACAAGAAGCG CGAGGCAGAA TTCCAGAAGA TGAGACGTGA CCTTGAAGAG 
GCCACTCTGC AACACGAAGC CACCGCTGCT GCTCTCAGAA AGAAGCAGGC CGACAGCGTG 
GCTGAACTCG GGGAACAAAT CGATAACCTG CAAAGAGTCA AGCAGAAGTT AGAAAAGGAG 
AAAAGTGAGC TAAAGATGGA GATTGATGAC CTGGCCAGCA ACATGGAAAA CCTATCCAAA 
GCCAAGGCCA ATTTTGAGAA GATTTGCCGC ACCCTTGAAG ATCAGCTGAG TGAACTCAAG 
ACAAAGGATG AGGAACACCA GAGGCAGATT AACGACCTCA CTGCCCAAAG GGCCCGACTG 
CAGACAGAAA ATGGTGAATA TGCACGACAG TTGGAGGAAA GAGATTCTTT GATCTCCCAG 
CTTTCTCGAG GCAAACAGGC ATTTACTCAA CAGATTGAAG AGCTCAAGAG GCAGCTGGAA 
GAGGAAATCA AGGCCAAGAA TGCCCTTGCC CATGGTCTGC AGTCAGCCCG CCATGATTGC 
GACTTGCTTA GAGAACAATA TGAGGAAGAG CAAGAGGCCA AGGGTGAGCT TCAGCGCTCG 
TTGTCCAAAG CCAATGGTGA AGTTGCCCAG TGGAGGACAA AGTACGAGAC AGACGCTATT 
CAGCGTACAG AAGAACTAGA GGATGCCAAG AAGAAACTGG CTCAGCGTCT CCAGGAAGCA 
GAAGAACACA TTGAAGCTGT GAACTCAAAG TGCGCTTCTC TCGAAAAGAC CAAACAGCGA 
CTTCAGAATG AAGTTGAAGA CCTTATGGTT GATGTAGAAC GGTCAAACAG TGCTTGTGCT 
GCTCTAGATA AGAAACAGAA GAACTTCGAT AAGATTCTGG CAGAATGGAA GCAGAAGTTT 
GAAGAGAGCC AGGCTGAACT GGAAGCCTCT CAGAAGGAGT CCCGGTCTCT CAGCACAGAG 
TTGTTCAAGA TGAAGAATGC CTATGAGGAA TCCCTGGATC ACCTCGAGAC AACCAAACGA 
GAGAACAAGA ACTTGCAGCA GGAGATTTCC GATCTTACTG AACAAGTTTC TGAAGTTTCC 
AAGACACTGC ACGAGGTGGA AAAAGCCAAG AAAATTATTG AGCAAGAAAA GACTGAAATT 
CAGTCTGCTC TAGAAGAAGC TGAGGCATCT CTGGAACACG AGGAAGGCAA GATACTGAGA 
ATCCAGCTGG AACTGAATCA AGTGAAATCA GAAATTGACA GAAAGATTGC AGAGAAGGAT 
GAGGAAATCG ATCAGTTGAA AAGGAACCAC GTCAGGGTGG TTGAGTCCAT GCAGAGCACA 
CTGGATGCTG AAATCAGAAG CAGGAACGAT GCTCTAAGGT TGAAGAAGAA AATGGAAGGA 
GATCTCAATG AAATGGAGAT CCAGTTGAGC CATGCCAATC GACAAGCTTC TGAGTCTCAG 
AAGCAGCTGA GAAATGTCCA GGGATTGCTC AAGGATGTTC AGATCCACTT GGACGACTCC 
CTGAGAGCCC AGGAAGATCT TAAGGAGCAA TTGGCTATGG TTGAACGCAG AGCTAATCTG 
ATGCAGGCTG AGATAGAAGA GATGAGGGCT GCTCTGGAAC AAACAGAACG GAGCAGGAAA 
GTCGCAGAAC AAGAACTACT GGATGCTAGT GAGCGTGTCC AGCTCCTGCA CTCACAGAAC 
ACAAGCTTGA TCAACACCAA GAAGAAGATG GAGACAGACA TCACTCAGCT ACAGACTGAG 
GTTGAAGAGG CCATACAAGA AGCCCGCAAT GCAGAAGAAA AGGCCAAAAA GGCCATAACA 
GATGCTGCCA TGATGGCAGA AGAGCTTAAA AAGGAACAGG ACACCAGTGC TCACTTGGAA 
AGGATGAAGA AGAACCTCGA TCAAACAGTG AAGGATCTGC AGCACCGTTT GGATGAAGCT 
GAACAACTGG CAATGAAAGG AGGAAAGAAA CAAATCCAGA AACTGGAGGC TAGGGTTCGG 
GAGCTTGAAA ATGAAGTGGA TGTAGAACAG AAACGCAGCA CAGAGGCCAT TAAAGGCGTC 
CGAAAATACG AACGGCGGGT TAAAGAACTA TCATACCAGT CTGAGGAGGA TAAGAAGAAT 
ATCCTGAGAC TTCAAGACCT GGTGGACAAA CTCCAAATGA AGGTGAAGGC CTACAAGAGG 
CAGACAGAGG AAATTGAGGA ACAGTCCAAT ATCAACCTCA CCAAATACCG GAAGATCCAA 
CATGAACTTG AGGAAGCCGA GGAACGTGCC GATATTGCAG AATCCCAGGT GAACAAGCTC 
CGCGCCAAGA CCCGAGAGGT CACCAAGATC ACGAAGAGCG AAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myh3 ( NM_001006914.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001006915.1 Xenopus tropicalis myosin, heavy chain 3, skeletal muscle, embryonic (myh3), mRNA.

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CloneID	OXa23509
Clone ID Related Accession (Same CDS sequence)	NM_001006914.1 , XM_012954178.3
Accession Version	NM_001006914.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-22
Organism	Xenopus tropicalis(tropical clawed frog)
Product	myosin heavy chain IIa
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC075407.1. ##Evidence-Data-START## Transcript exon combination :: BC075407.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGAGTTCGG ACGCGGAGAT GGCCGTTTTC GGGCCAGCAG CCCATTTTCT CAGAAAAAGT 
GAGAAAGAGC GGATCGAGGC GCAGAACAAA CCATTCGACG CAAGGACCTC CATGTTTGTG 
GTGGATACCA AGGAATCCTA CGTGAAAGGG ACCCTCCAAA GCAAGGAGGG CGGGAAGGCT 
ACTGTAAAGA CAGAAGCGGG ACAGACTGTA ACAGTGAAGG ATGATCAGAT CTTCCCCATG 
AACCCACCAA AGTTCGATAA AATTGAAGAC ATGGCCATGA TGACCCACTT GAACGAACCA 
TCTGTCCTGT ATAACCTCAA AGAGCGTTAC GCAGCCTGGA TGATCTACAC CTATTCTGGG 
CTCTTCTGCG TCACTGTCAA CCCCTACAAG TGGCTTCCCG TGTACAACCC AGAGGTGGTC 
AATGCCTACC GTGGGAAGAA GCGTCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAT 
AACGCCTATC AGTTCATGTT AACTGATCGT GAGAACCAGT CTATCCTGAT CACTGGAGAA 
TCCGGTGCCG GGAAGACCGT AAACACCAAG CGTGTCATTC AGTATTTTGC AACAATTGCA 
GCCGCCCCCG ACAAGAAAAA AGAAGAGGTG GCAAAGACCA AGGGGACCCT GGAGGATCAA 
ATCATTCAGG CCAACCCGCT GCTGGAAGCC TTCGGCAATG CCAAGACCGT CAGGAACGAC 
AACTCTTCCC GCTTTGGTAA ATTCATCAGA ATCCATTTCG GCACGACTGG GAAACTCGCC 
TCTGCTGATA TTGAAACATA CCTGCTGGAG AAGTCCAGAG TTACCTTCCA GCTAAAGGCC 
GAGAGGAGCT ATCACATTTT CTACCAAATC ATGTCCAACA AAAAGCCCGA ACTGATTGAA 
ATGCTCCTCA TCACCACCAA CCCCTACGAC TTTCCATTCG TCAGCCAAGG GGAGATCTCT 
GTGGCCAGTA TCGACGATAC GGAGGAACTC ATGGCCACTG ATAGCGCCAT CGATATCCTG 
GGCTTCAATG CGGATGAGAA AGTTTCTATC TATAAACTGA CCGGTGCCGT CATGCACTAC 
GGGAACATGA GATTCAAGCA GAAACAGCGA GAGGAGCAGG CCGAGCCGGA CGGCACAGAA 
GTGGCCGACA AAGCTGCCTA CTTGATGTGC CTGAACTCAG CAGATCTGCT GAAGGCTCTG 
TGCTACCCAA GGGTCAAGGT CGGCAATGAA TATGTCACCA AGGGGCAAAC TGTCCAGCAG 
GTGTACAACT CAGTCGGCGC TCTGGGCAAA GCTGTTTACG AGAAGATGTT CTTGTGGATG 
GTCACGCGTA TCAATGAAAT GTTGGACACC AAGCAGCCAA GGCAGTATTT CATCGGTGTG 
CTGGACATCG CTGGCTTTGA GATCTTTGAT TTCAACAGCT TGGAGCAGCT CTGCATCAAC 
TTCACCAACG AGAAACTGCA ACAGTTTTTC AACCACCACA TGTTTGTCCT GGAGCAAGAG 
GAGTACAAGA AGGAGGGGAT CGAGTGGGAG TTCATTGACT TCGGCATGGA CTTGGCCGCC 
TGCATTGAGC TTATTGAGAA GCCAATGGGC ATTTTCTCCA TCCTTGAAGA GGAGTGCATG 
TTCCCCAAGG CAACAGACAC TTCATTCAAG AACAAGTTGT ACGACCAACA TCTGGGCAAG 
TCCAACAACT TCCAGAAGCC CAAACCGGCC AAAGGCAAAG CCGAGGCCCA CTTCTCTCTT 
GTCCACTATG CCGGGACGGT AGATTATAAC ATCAGTGGTT GGCTTGAAAA GAACAAGGAC 
CCACTGAATG ACACTGTCAT TGGCCTCTTC CAGAAGTCTT CAATGAAGAC ACTGGCGTAT 
CTGTTCTCCA GCTATGCTGC TTCTGAAGCA GAGGGTGCAA AGAAAGGTGG TAAGAAGAAG 
GGGTCTTCTT TCCAGACCGT GTCTGCTCTT TTTAGGGAAA ATCTGAATAA GCTAATGAGC 
AATCTAAGGA CCACCCATCC TCACTTTGTC CGTTGCATTA TTCCCAATGA GACCAAAACT 
CCAGGGGCAA TGGAACATGA ACTTGTATTG CATCAGCTCA GGTGTAATGG GGTGCTGGAG 
GGGATCAGGA TTTGCAGAAA GGGATTCCCA AGCAGGATTC TCTACGGTGA CTTCAAGCAA 
AGATACAAAA TTTTAAATGC AAGTGCCATC CCAGAGGGGC AGTTTATTGA CAGCAAGAAG 
GCCTCTGAAA AACTTCTTGG ATCCATTGAT GTTGATCATA CCCAGTACAG ATTTGGCCAC 
ACTAAGGTGT TCTTCAAAGC TGGTCTTCTG GGTACCCTGG AAGAGATGCG AGACGATCGC 
TTGGCCCATG TAATCACACG TACCCAGGCT ATGTGCAGAG GGTACCTCAT GAGGGTGGAG 
TTTAAGAAAA TGATGGAGAG AAGGGAAGCT GTGTTCACTA TTCAATACAA TATCCGGTCC 
TTCATGAATG TCAAGCATTG GCCATGGATG AAGCTTTATT TCAAGATCAA GCCCCTCCTG 
AAGAGTGCCG AGTCTGAGAA AGAGATGCAG AACATGAAGG AAGAGTTTGA GAAGACCAAG 
GAGCTGCTGG CCAAGTCAGA AGCTAAACGT AAGGAGCTGG AAGAGAAGAT GGTTAAAGTT 
CTCCAAGAGA AGAATGACCT GCAACTTCAA GTCCAGTCTG AATCAGAAAA TCTCACAGAT 
GCCGAGGAAA GGTGCGATCA GTTAATCAAA TCCAAAATCC AGTTAGAAGC CAAAATGAAA 
GAGCTGAATG AGAGGCTGGA AGACGAGGAG GAATCCAACG CTGAACTGAC AGCCAAGAAG 
AGGAAACTTG AAGATGAATG CTCAGAACTG AAGAAAGATA TTGACGATCT GGAGTTAACG 
CTGGCCAAAG TTGAAAAAGA GAAACACGCA ACAGAGAACA AGGTTAAGAA CCTCACTGAA 
GAAATGGCAG TTTTGGATGA GACCATCGTT AAGCTTACCA AAGAGAAAAA AGCCCTTCAA 
GAAGCTCACC AGCAGACCCT TGATGACCTC CAGGCGGAGG AAGACAAAGT CAACACTCTG 
ACCAAAGCCA AGGCCAAGCT TGAGCAGCAA GTCGACGATC TTGAAGGGTC TTTGGAACAA 
GAGAAGAAAC TGCGCATGGA CCTTGAAAGA TCTAAGAGAA AGCTAGAGGG TGATCTTAAA 
CTTGCCCAGG AAACCATCAT GGACCTGGAA AACGATAGGC AGCAACTGGA TGAAAAGCTA 
AAAAAGAAAG AATTTGAAAT GAGCCAGTAC GCAAGCAAAA TTGAGGATGA GCAGGCTCTT 
GGTGCTCAAC TGCAGAAAAA GATTAAGGAA TTGCAGGCCC GCATTGAGGA GCTAGAAGAA 
GAAATAGAAG CTGAGCGTGC AGCTCGTGCC AAGGCAGAGA AGCAGCGTTC CGACCTCTCC 
AGGGAACTTG AGGAGATCAG TGAGCGCCTG GAGGAAGCTG GAGGTGCCAC CTCTGCTCAG 
ATTGAGATGA ACAAGAAGCG CGAGGCAGAA TTCCAGAAGA TGAGACGTGA CCTTGAAGAG 
GCCACTCTGC AACACGAAGC CACCGCTGCT GCTCTCAGAA AGAAGCAGGC CGACAGCGTG 
GCTGAACTCG GGGAACAAAT CGATAACCTG CAAAGAGTCA AGCAGAAGTT AGAAAAGGAG 
AAAAGTGAGC TAAAGATGGA GATTGATGAC CTGGCCAGCA ACATGGAAAA CCTATCCAAA 
GCCAAGGCCA ATTTTGAGAA GATTTGCCGC ACCCTTGAAG ATCAGCTGAG TGAACTCAAG 
ACAAAGGATG AGGAACACCA GAGGCAGATT AACGACCTCA CTGCCCAAAG GGCCCGACTG 
CAGACAGAAA ATGGTGAATA TGCACGACAG TTGGAGGAAA GAGATTCTTT GATCTCCCAG 
CTTTCTCGAG GCAAACAGGC ATTTACTCAA CAGATTGAAG AGCTCAAGAG GCAGCTGGAA 
GAGGAAATCA AGGCCAAGAA TGCCCTTGCC CATGGTCTGC AGTCAGCCCG CCATGATTGC 
GACTTGCTTA GAGAACAATA TGAGGAAGAG CAAGAGGCCA AGGGTGAGCT TCAGCGCTCG 
TTGTCCAAAG CCAATGGTGA AGTTGCCCAG TGGAGGACAA AGTACGAGAC AGACGCTATT 
CAGCGTACAG AAGAACTAGA GGATGCCAAG AAGAAACTGG CTCAGCGTCT CCAGGAAGCA 
GAAGAACACA TTGAAGCTGT GAACTCAAAG TGCGCTTCTC TCGAAAAGAC CAAACAGCGA 
CTTCAGAATG AAGTTGAAGA CCTTATGGTT GATGTAGAAC GGTCAAACAG TGCTTGTGCT 
GCTCTAGATA AGAAACAGAA GAACTTCGAT AAGATTCTGG CAGAATGGAA GCAGAAGTTT 
GAAGAGAGCC AGGCTGAACT GGAAGCCTCT CAGAAGGAGT CCCGGTCTCT CAGCACAGAG 
TTGTTCAAGA TGAAGAATGC CTATGAGGAA TCCCTGGATC ACCTCGAGAC AACCAAACGA 
GAGAACAAGA ACTTGCAGCA GGAGATTTCC GATCTTACTG AACAAGTTTC TGAAGTTTCC 
AAGACACTGC ACGAGGTGGA AAAAGCCAAG AAAATTATTG AGCAAGAAAA GACTGAAATT 
CAGTCTGCTC TAGAAGAAGC TGAGGCATCT CTGGAACACG AGGAAGGCAA GATACTGAGA 
ATCCAGCTGG AACTGAATCA AGTGAAATCA GAAATTGACA GAAAGATTGC AGAGAAGGAT 
GAGGAAATCG ATCAGTTGAA AAGGAACCAC GTCAGGGTGG TTGAGTCCAT GCAGAGCACA 
CTGGATGCTG AAATCAGAAG CAGGAACGAT GCTCTAAGGT TGAAGAAGAA AATGGAAGGA 
GATCTCAATG AAATGGAGAT CCAGTTGAGC CATGCCAATC GACAAGCTTC TGAGTCTCAG 
AAGCAGCTGA GAAATGTCCA GGGATTGCTC AAGGATGTTC AGATCCACTT GGACGACTCC 
CTGAGAGCCC AGGAAGATCT TAAGGAGCAA TTGGCTATGG TTGAACGCAG AGCTAATCTG 
ATGCAGGCTG AGATAGAAGA GATGAGGGCT GCTCTGGAAC AAACAGAACG GAGCAGGAAA 
GTCGCAGAAC AAGAACTACT GGATGCTAGT GAGCGTGTCC AGCTCCTGCA CTCACAGAAC 
ACAAGCTTGA TCAACACCAA GAAGAAGATG GAGACAGACA TCACTCAGCT ACAGACTGAG 
GTTGAAGAGG CCATACAAGA AGCCCGCAAT GCAGAAGAAA AGGCCAAAAA GGCCATAACA 
GATGCTGCCA TGATGGCAGA AGAGCTTAAA AAGGAACAGG ACACCAGTGC TCACTTGGAA 
AGGATGAAGA AGAACCTCGA TCAAACAGTG AAGGATCTGC AGCACCGTTT GGATGAAGCT 
GAACAACTGG CAATGAAAGG AGGAAAGAAA CAAATCCAGA AACTGGAGGC TAGGGTTCGG 
GAGCTTGAAA ATGAAGTGGA TGTAGAACAG AAACGCAGCA CAGAGGCCAT TAAAGGCGTC 
CGAAAATACG AACGGCGGGT TAAAGAACTA TCATACCAGT CTGAGGAGGA TAAGAAGAAT 
ATCCTGAGAC TTCAAGACCT GGTGGACAAA CTCCAAATGA AGGTGAAGGC CTACAAGAGG 
CAGACAGAGG AAATTGAGGA ACAGTCCAAT ATCAACCTCA CCAAATACCG GAAGATCCAA 
CATGAACTTG AGGAAGCCGA GGAACGTGCC GATATTGCAG AATCCCAGGT GAACAAGCTC 
CGCGCCAAGA CCCGAGAGGT CACCAAGATC ACGAAGAGCG AAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001006915.1
CDS	66..5873
Translation	MSSDAEMAVFGPAAHFLRKSEKERIEAQNKPFDARTSMFVVDTKESYVKGTLQSKEGGKATVKTEAGQTVTVKDDQIFPMNPPKFDKIEDMAMMTHLNEPSVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPEVVNAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAAAPDKKKEEVAKTKGTLEDQIIQANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQIMSNKKPELIEMLLITTNPYDFPFVSQGEISVASIDDTEELMATDSAIDILGFNADEKVSIYKLTGAVMHYGNMRFKQKQREEQAEPDGTEVADKAAYLMCLNSADLLKALCYPRVKVGNEYVTKGQTVQQVYNSVGALGKAVYEKMFLWMVTRINEMLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDQHLGKSNNFQKPKPAKGKAEAHFSLVHYAGTVDYNISGWLEKNKDPLNDTVIGLFQKSSMKTLAYLFSSYAASEAEGAKKGGKKKGSSFQTVSALFRENLNKLMSNLRTTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYGDFKQRYKILNASAIPEGQFIDSKKASEKLLGSIDVDHTQYRFGHTKVFFKAGLLGTLEEMRDDRLAHVITRTQAMCRGYLMRVEFKKMMERREAVFTIQYNIRSFMNVKHWPWMKLYFKIKPLLKSAESEKEMQNMKEEFEKTKELLAKSEAKRKELEEKMVKVLQEKNDLQLQVQSESENLTDAEERCDQLIKSKIQLEAKMKELNERLEDEEESNAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAVLDETIVKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKAKLEQQVDDLEGSLEQEKKLRMDLERSKRKLEGDLKLAQETIMDLENDRQQLDEKLKKKEFEMSQYASKIEDEQALGAQLQKKIKELQARIEELEEEIEAERAARAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKQADSVAELGEQIDNLQRVKQKLEKEKSELKMEIDDLASNMENLSKAKANFEKICRTLEDQLSELKTKDEEHQRQINDLTAQRARLQTENGEYARQLEERDSLISQLSRGKQAFTQQIEELKRQLEEEIKAKNALAHGLQSARHDCDLLREQYEEEQEAKGELQRSLSKANGEVAQWRTKYETDAIQRTEELEDAKKKLAQRLQEAEEHIEAVNSKCASLEKTKQRLQNEVEDLMVDVERSNSACAALDKKQKNFDKILAEWKQKFEESQAELEASQKESRSLSTELFKMKNAYEESLDHLETTKRENKNLQQEISDLTEQVSEVSKTLHEVEKAKKIIEQEKTEIQSALEEAEASLEHEEGKILRIQLELNQVKSEIDRKIAEKDEEIDQLKRNHVRVVESMQSTLDAEIRSRNDALRLKKKMEGDLNEMEIQLSHANRQASESQKQLRNVQGLLKDVQIHLDDSLRAQEDLKEQLAMVERRANLMQAEIEEMRAALEQTERSRKVAEQELLDASERVQLLHSQNTSLINTKKKMETDITQLQTEVEEAIQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLDQTVKDLQHRLDEAEQLAMKGGKKQIQKLEARVRELENEVDVEQKRSTEAIKGVRKYERRVKELSYQSEEDKKNILRLQDLVDKLQMKVKAYKRQTEEIEEQSNINLTKYRKIQHELEEAEERADIAESQVNKLRAKTREVTKITKSEE

Target ORF information:

RefSeq Version	NM_001006914.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin, heavy chain 3, skeletal muscle, embryonic (myh3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001006914.1

ORF Insert Sequence:

ATGAGTTCGG ACGCGGAGAT GGCCGTTTTC GGGCCAGCAG CCCATTTTCT CAGAAAAAGT 
GAGAAAGAGC GGATCGAGGC GCAGAACAAA CCATTCGACG CAAGGACCTC CATGTTTGTG 
GTGGATACCA AGGAATCCTA CGTGAAAGGG ACCCTCCAAA GCAAGGAGGG CGGGAAGGCT 
ACTGTAAAGA CAGAAGCGGG ACAGACTGTA ACAGTGAAGG ATGATCAGAT CTTCCCCATG 
AACCCACCAA AGTTCGATAA AATTGAAGAC ATGGCCATGA TGACCCACTT GAACGAACCA 
TCTGTCCTGT ATAACCTCAA AGAGCGTTAC GCAGCCTGGA TGATCTACAC CTATTCTGGG 
CTCTTCTGCG TCACTGTCAA CCCCTACAAG TGGCTTCCCG TGTACAACCC AGAGGTGGTC 
AATGCCTACC GTGGGAAGAA GCGTCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAT 
AACGCCTATC AGTTCATGTT AACTGATCGT GAGAACCAGT CTATCCTGAT CACTGGAGAA 
TCCGGTGCCG GGAAGACCGT AAACACCAAG CGTGTCATTC AGTATTTTGC AACAATTGCA 
GCCGCCCCCG ACAAGAAAAA AGAAGAGGTG GCAAAGACCA AGGGGACCCT GGAGGATCAA 
ATCATTCAGG CCAACCCGCT GCTGGAAGCC TTCGGCAATG CCAAGACCGT CAGGAACGAC 
AACTCTTCCC GCTTTGGTAA ATTCATCAGA ATCCATTTCG GCACGACTGG GAAACTCGCC 
TCTGCTGATA TTGAAACATA CCTGCTGGAG AAGTCCAGAG TTACCTTCCA GCTAAAGGCC 
GAGAGGAGCT ATCACATTTT CTACCAAATC ATGTCCAACA AAAAGCCCGA ACTGATTGAA 
ATGCTCCTCA TCACCACCAA CCCCTACGAC TTTCCATTCG TCAGCCAAGG GGAGATCTCT 
GTGGCCAGTA TCGACGATAC GGAGGAACTC ATGGCCACTG ATAGCGCCAT CGATATCCTG 
GGCTTCAATG CGGATGAGAA AGTTTCTATC TATAAACTGA CCGGTGCCGT CATGCACTAC 
GGGAACATGA GATTCAAGCA GAAACAGCGA GAGGAGCAGG CCGAGCCGGA CGGCACAGAA 
GTGGCCGACA AAGCTGCCTA CTTGATGTGC CTGAACTCAG CAGATCTGCT GAAGGCTCTG 
TGCTACCCAA GGGTCAAGGT CGGCAATGAA TATGTCACCA AGGGGCAAAC TGTCCAGCAG 
GTGTACAACT CAGTCGGCGC TCTGGGCAAA GCTGTTTACG AGAAGATGTT CTTGTGGATG 
GTCACGCGTA TCAATGAAAT GTTGGACACC AAGCAGCCAA GGCAGTATTT CATCGGTGTG 
CTGGACATCG CTGGCTTTGA GATCTTTGAT TTCAACAGCT TGGAGCAGCT CTGCATCAAC 
TTCACCAACG AGAAACTGCA ACAGTTTTTC AACCACCACA TGTTTGTCCT GGAGCAAGAG 
GAGTACAAGA AGGAGGGGAT CGAGTGGGAG TTCATTGACT TCGGCATGGA CTTGGCCGCC 
TGCATTGAGC TTATTGAGAA GCCAATGGGC ATTTTCTCCA TCCTTGAAGA GGAGTGCATG 
TTCCCCAAGG CAACAGACAC TTCATTCAAG AACAAGTTGT ACGACCAACA TCTGGGCAAG 
TCCAACAACT TCCAGAAGCC CAAACCGGCC AAAGGCAAAG CCGAGGCCCA CTTCTCTCTT 
GTCCACTATG CCGGGACGGT AGATTATAAC ATCAGTGGTT GGCTTGAAAA GAACAAGGAC 
CCACTGAATG ACACTGTCAT TGGCCTCTTC CAGAAGTCTT CAATGAAGAC ACTGGCGTAT 
CTGTTCTCCA GCTATGCTGC TTCTGAAGCA GAGGGTGCAA AGAAAGGTGG TAAGAAGAAG 
GGGTCTTCTT TCCAGACCGT GTCTGCTCTT TTTAGGGAAA ATCTGAATAA GCTAATGAGC 
AATCTAAGGA CCACCCATCC TCACTTTGTC CGTTGCATTA TTCCCAATGA GACCAAAACT 
CCAGGGGCAA TGGAACATGA ACTTGTATTG CATCAGCTCA GGTGTAATGG GGTGCTGGAG 
GGGATCAGGA TTTGCAGAAA GGGATTCCCA AGCAGGATTC TCTACGGTGA CTTCAAGCAA 
AGATACAAAA TTTTAAATGC AAGTGCCATC CCAGAGGGGC AGTTTATTGA CAGCAAGAAG 
GCCTCTGAAA AACTTCTTGG ATCCATTGAT GTTGATCATA CCCAGTACAG ATTTGGCCAC 
ACTAAGGTGT TCTTCAAAGC TGGTCTTCTG GGTACCCTGG AAGAGATGCG AGACGATCGC 
TTGGCCCATG TAATCACACG TACCCAGGCT ATGTGCAGAG GGTACCTCAT GAGGGTGGAG 
TTTAAGAAAA TGATGGAGAG AAGGGAAGCT GTGTTCACTA TTCAATACAA TATCCGGTCC 
TTCATGAATG TCAAGCATTG GCCATGGATG AAGCTTTATT TCAAGATCAA GCCCCTCCTG 
AAGAGTGCCG AGTCTGAGAA AGAGATGCAG AACATGAAGG AAGAGTTTGA GAAGACCAAG 
GAGCTGCTGG CCAAGTCAGA AGCTAAACGT AAGGAGCTGG AAGAGAAGAT GGTTAAAGTT 
CTCCAAGAGA AGAATGACCT GCAACTTCAA GTCCAGTCTG AATCAGAAAA TCTCACAGAT 
GCCGAGGAAA GGTGCGATCA GTTAATCAAA TCCAAAATCC AGTTAGAAGC CAAAATGAAA 
GAGCTGAATG AGAGGCTGGA AGACGAGGAG GAATCCAACG CTGAACTGAC AGCCAAGAAG 
AGGAAACTTG AAGATGAATG CTCAGAACTG AAGAAAGATA TTGACGATCT GGAGTTAACG 
CTGGCCAAAG TTGAAAAAGA GAAACACGCA ACAGAGAACA AGGTTAAGAA CCTCACTGAA 
GAAATGGCAG TTTTGGATGA GACCATCGTT AAGCTTACCA AAGAGAAAAA AGCCCTTCAA 
GAAGCTCACC AGCAGACCCT TGATGACCTC CAGGCGGAGG AAGACAAAGT CAACACTCTG 
ACCAAAGCCA AGGCCAAGCT TGAGCAGCAA GTCGACGATC TTGAAGGGTC TTTGGAACAA 
GAGAAGAAAC TGCGCATGGA CCTTGAAAGA TCTAAGAGAA AGCTAGAGGG TGATCTTAAA 
CTTGCCCAGG AAACCATCAT GGACCTGGAA AACGATAGGC AGCAACTGGA TGAAAAGCTA 
AAAAAGAAAG AATTTGAAAT GAGCCAGTAC GCAAGCAAAA TTGAGGATGA GCAGGCTCTT 
GGTGCTCAAC TGCAGAAAAA GATTAAGGAA TTGCAGGCCC GCATTGAGGA GCTAGAAGAA 
GAAATAGAAG CTGAGCGTGC AGCTCGTGCC AAGGCAGAGA AGCAGCGTTC CGACCTCTCC 
AGGGAACTTG AGGAGATCAG TGAGCGCCTG GAGGAAGCTG GAGGTGCCAC CTCTGCTCAG 
ATTGAGATGA ACAAGAAGCG CGAGGCAGAA TTCCAGAAGA TGAGACGTGA CCTTGAAGAG 
GCCACTCTGC AACACGAAGC CACCGCTGCT GCTCTCAGAA AGAAGCAGGC CGACAGCGTG 
GCTGAACTCG GGGAACAAAT CGATAACCTG CAAAGAGTCA AGCAGAAGTT AGAAAAGGAG 
AAAAGTGAGC TAAAGATGGA GATTGATGAC CTGGCCAGCA ACATGGAAAA CCTATCCAAA 
GCCAAGGCCA ATTTTGAGAA GATTTGCCGC ACCCTTGAAG ATCAGCTGAG TGAACTCAAG 
ACAAAGGATG AGGAACACCA GAGGCAGATT AACGACCTCA CTGCCCAAAG GGCCCGACTG 
CAGACAGAAA ATGGTGAATA TGCACGACAG TTGGAGGAAA GAGATTCTTT GATCTCCCAG 
CTTTCTCGAG GCAAACAGGC ATTTACTCAA CAGATTGAAG AGCTCAAGAG GCAGCTGGAA 
GAGGAAATCA AGGCCAAGAA TGCCCTTGCC CATGGTCTGC AGTCAGCCCG CCATGATTGC 
GACTTGCTTA GAGAACAATA TGAGGAAGAG CAAGAGGCCA AGGGTGAGCT TCAGCGCTCG 
TTGTCCAAAG CCAATGGTGA AGTTGCCCAG TGGAGGACAA AGTACGAGAC AGACGCTATT 
CAGCGTACAG AAGAACTAGA GGATGCCAAG AAGAAACTGG CTCAGCGTCT CCAGGAAGCA 
GAAGAACACA TTGAAGCTGT GAACTCAAAG TGCGCTTCTC TCGAAAAGAC CAAACAGCGA 
CTTCAGAATG AAGTTGAAGA CCTTATGGTT GATGTAGAAC GGTCAAACAG TGCTTGTGCT 
GCTCTAGATA AGAAACAGAA GAACTTCGAT AAGATTCTGG CAGAATGGAA GCAGAAGTTT 
GAAGAGAGCC AGGCTGAACT GGAAGCCTCT CAGAAGGAGT CCCGGTCTCT CAGCACAGAG 
TTGTTCAAGA TGAAGAATGC CTATGAGGAA TCCCTGGATC ACCTCGAGAC AACCAAACGA 
GAGAACAAGA ACTTGCAGCA GGAGATTTCC GATCTTACTG AACAAGTTTC TGAAGTTTCC 
AAGACACTGC ACGAGGTGGA AAAAGCCAAG AAAATTATTG AGCAAGAAAA GACTGAAATT 
CAGTCTGCTC TAGAAGAAGC TGAGGCATCT CTGGAACACG AGGAAGGCAA GATACTGAGA 
ATCCAGCTGG AACTGAATCA AGTGAAATCA GAAATTGACA GAAAGATTGC AGAGAAGGAT 
GAGGAAATCG ATCAGTTGAA AAGGAACCAC GTCAGGGTGG TTGAGTCCAT GCAGAGCACA 
CTGGATGCTG AAATCAGAAG CAGGAACGAT GCTCTAAGGT TGAAGAAGAA AATGGAAGGA 
GATCTCAATG AAATGGAGAT CCAGTTGAGC CATGCCAATC GACAAGCTTC TGAGTCTCAG 
AAGCAGCTGA GAAATGTCCA GGGATTGCTC AAGGATGTTC AGATCCACTT GGACGACTCC 
CTGAGAGCCC AGGAAGATCT TAAGGAGCAA TTGGCTATGG TTGAACGCAG AGCTAATCTG 
ATGCAGGCTG AGATAGAAGA GATGAGGGCT GCTCTGGAAC AAACAGAACG GAGCAGGAAA 
GTCGCAGAAC AAGAACTACT GGATGCTAGT GAGCGTGTCC AGCTCCTGCA CTCACAGAAC 
ACAAGCTTGA TCAACACCAA GAAGAAGATG GAGACAGACA TCACTCAGCT ACAGACTGAG 
GTTGAAGAGG CCATACAAGA AGCCCGCAAT GCAGAAGAAA AGGCCAAAAA GGCCATAACA 
GATGCTGCCA TGATGGCAGA AGAGCTTAAA AAGGAACAGG ACACCAGTGC TCACTTGGAA 
AGGATGAAGA AGAACCTCGA TCAAACAGTG AAGGATCTGC AGCACCGTTT GGATGAAGCT 
GAACAACTGG CAATGAAAGG AGGAAAGAAA CAAATCCAGA AACTGGAGGC TAGGGTTCGG 
GAGCTTGAAA ATGAAGTGGA TGTAGAACAG AAACGCAGCA CAGAGGCCAT TAAAGGCGTC 
CGAAAATACG AACGGCGGGT TAAAGAACTA TCATACCAGT CTGAGGAGGA TAAGAAGAAT 
ATCCTGAGAC TTCAAGACCT GGTGGACAAA CTCCAAATGA AGGTGAAGGC CTACAAGAGG 
CAGACAGAGG AAATTGAGGA ACAGTCCAAT ATCAACCTCA CCAAATACCG GAAGATCCAA 
CATGAACTTG AGGAAGCCGA GGAACGTGCC GATATTGCAG AATCCCAGGT GAACAAGCTC 
CGCGCCAAGA CCCGAGAGGT CACCAAGATC ACGAAGAGCG AAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myh3 ( XM_012954178.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012809632.1 Xenopus tropicalis myosin, heavy chain 3, skeletal muscle, embryonic (myh3), transcript variant X1, mRNA.

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CloneID	OXa26781
Clone ID Related Accession (Same CDS sequence)	XM_012954178.2
Accession Version	XM_012954178.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	myosin heavy chain IIa isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012954178.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCGG ACGCGGAGAT GGCCGTTTTC GGGCCAGCAG CCCATTTTCT CAGAAAAAGT 
GAGAAAGAGC GGATCGAGGC GCAGAACAAA CCATTCGACG CAAGGACCTC CATGTTTGTG 
GTGGATACCA AGGAATCCTA CGTGAAAGGG ACCCTCCAAA GCAAGGAGGG CGGGAAGGCT 
ACTGTAAAGA CAGAAGCGGG ACAGACTGTA ACAGTGAAGG ATGATCAGAT CTTCCCCATG 
AACCCACCAA AGTTCGATAA AATTGAAGAC ATGGCCATGA TGACCCACTT GAACGAACCA 
TCTGTCCTGT ATAACCTCAA AGAGCGTTAC GCAGCCTGGA TGATCTACAC CTATTCTGGG 
CTCTTCTGCG TCACTGTCAA CCCCTACAAG TGGCTTCCCG TGTACAACCC AGAGGTGGTC 
AATGCCTACC GTGGGAAGAA GCGTCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAT 
AACGCCTATC AGTTCATGTT AACTGATCGT GAGAACCAGT CTATCCTGAT CACTGGAGAA 
TCCGGTGCCG GGAAGACCGT AAACACCAAG CGTGTCATTC AGTATTTTGC AACAATTGCA 
GCCGCCCCCG ACAAGAAAAA AGAAGAGGTG GCAAAGACCA AGGGGACCCT GGAGGATCAA 
ATCATTCAGG CCAACCCGCT GCTGGAAGCC TTCGGCAATG CCAAGACCGT CAGGAACGAC 
AACTCTTCCC GCTTTGGTAA ATTCATCAGA ATCCATTTCG GCACGACTGG GAAACTCGCC 
TCTGCTGATA TTGAAACATA CCTGCTGGAG AAGTCCAGAG TTACCTTCCA GCTAAAGGCC 
GAGAGGAGCT ATCACATTTT CTACCAAATC ATGTCCAACA AAAAGCCCGA ACTGATTGAA 
ATGCTCCTCA TCACCACCAA CCCCTACGAC TTTCCATTCG TCAGCCAAGG GGAGATCTCT 
GTGGCCAGTA TCGACGATAC GGAGGAACTC ATGGCCACTG ATAGCGCCAT CGATATCCTG 
GGCTTCAATG CGGATGAGAA AGTTTCTATC TATAAACTGA CCGGTGCCGT CATGCACTAC 
GGGAACATGA GATTCAAGCA GAAACAGCGA GAGGAGCAGG CCGAGCCGGA CGGCACAGAA 
GTGGCCGACA AAGCTGCCTA TTTGATGTGC CTGAACTCAG CAGATCTGCT GAAGGCTCTG 
TGCTACCCAA GGGTCAAGGT CGGCAATGAA TATGTCACCA AGGGGCAAAC TGTCCAGCAG 
GTGTACAACT CAGTCGGCGC TCTGGGCAAA GCTGTTTACG AGAAGATGTT CTTGTGGATG 
GTCACGCGTA TCAATGAAAT GTTGGACACC AAGCAGCCAA GGCAGTATTT CATCGGTGTG 
CTGGACATCG CTGGCTTTGA GATCTTTGAT TTCAACAGCT TGGAGCAGCT CTGCATCAAC 
TTCACCAACG AGAAACTGCA ACAGTTTTTC AACCACCACA TGTTTGTCCT GGAGCAAGAG 
GAGTACAAGA AGGAGGGGAT CGAGTGGGAG TTCATTGACT TCGGCATGGA CTTGGCCGCC 
TGCATTGAGC TTATTGAGAA GCCAATGGGC ATTTTCTCCA TCCTTGAAGA GGAGTGCATG 
TTCCCCAAGG CAACAGACAC TTCATTCAAG AACAAGTTGT ACGACCAACA TCTGGGCAAG 
TCCAACAACT TCCAGAAGCC CAAACCGGCC AAAGGCAAAG CCGAGGCCCA CTTCTCTCTT 
GTCCACTATG CCGGGACGGT AGATTATAAC ATCAGTGGTT GGCTTGAAAA GAACAAGGAC 
CCACTGAATG ACACTGTCAT TGGCCTCTTC CAGAAGTCTT CAATGAAGAC ACTGGCGTAT 
CTGTTCTCCA GCTATGCTGC TTCTGAAGCA GAGGGTGCAA AGAAAGGTGG TAAGAAGAAG 
GGGTCTTCTT TCCAGACCGT GTCTGCTCTT TTTAGGGAAA ATCTGAATAA GCTAATGAGC 
AATCTGAGGA CCACCCATCC TCACTTTGTC CGTTGCATTA TTCCCAATGA GACCAAAACT 
CCAGGGGCAA TGGAACATGA ACTTGTATTG CATCAGCTCA GGTGTAATGG GGTGCTGGAG 
GGGATCAGGA TTTGCAGAAA GGGATTCCCA AGCAGGATTC TCTACGGTGA CTTCAAGCAA 
AGATACAAAA TTTTAAATGC AAGTGCCATC CCAGAGGGGC AGTTTATTGA CAGCAAGAAG 
GCCTCTGAAA AACTTCTTGG ATCCATTGAT GTTGATCATA CCCAGTACAG ATTTGGCCAC 
ACTAAGGTGT TCTTCAAAGC TGGTCTTCTG GGTACCCTGG AAGAGATGCG AGACGATCGC 
TTGGCCCATG TAATCACACG TACCCAGGCT ATGTGCAGAG GGTACCTCAT GAGGGTGGAG 
TTTAAGAAAA TGATGGAGAG AAGGGAAGCT GTGTTCACTA TTCAATACAA TATCCGGTCC 
TTCATGAATG TCAAGCATTG GCCATGGATG AAGCTTTATT TCAAGATCAA GCCTCTCCTG 
AAGAGTGCCG AGTCCGAGAA AGAGATGCAG AACATGAAGG AAGAGTTTGA GAAGACCAAG 
GAGCTGCTGG CCAAGTCAGA AGCTAAACGT AAGGAGCTGG AAGAGAAGAT GGTTAAAGTT 
CTCCAAGAGA AGAATGACCT GCAACTTCAA GTCCAGTCTG AATCAGAAAA TCTCACAGAT 
GCCGAGGAAA GGTGCGATCA GTTAATCAAA TCCAAAATCC AGTTAGAAGC CAAAATGAAA 
GAGCTGAATG AGAGGCTGGA AGACGAGGAG GAATCCAACG CTGAACTGAC AGCCAAGAAG 
AGGAAACTTG AAGATGAATG CTCAGAACTG AAGAAAGATA TTGACGATCT GGAGTTAACG 
CTGGCCAAAG TTGAAAAAGA GAAACACGCA ACAGAGAACA AGGTTAAGAA CCTCACTGAA 
GAAATGGCAG TTTTGGATGA GACCATCGTT AAGCTTACCA AAGAGAAAAA AGCCCTTCAA 
GAAGCTCACC AGCAGACCCT TGATGACCTC CAGGCGGAGG AAGACAAAGT CAACACTCTG 
ACCAAAGCCA AGGCCAAGCT TGAGCAGCAA GTCGACGATC TTGAAGGGTC TTTGGAACAA 
GAGAAGAAAC TGCGCATGGA CCTTGAAAGA TCTAAGAGAA AGCTAGAGGG TGATCTTAAA 
CTTGCCCAGG AAACCATCAT GGACCTGGAA AACGATAGGC AGCAACTGGA TGAAAAGCTA 
AAAAAGAAAG AATTTGAAAT GAGCCAGTAC GCAAGCAAAA TTGAGGATGA GCAGGCTCTT 
GGTGCTCAAC TGCAGAAAAA GATTAAGGAA TTGCAGGCCC GCATTGAGGA GCTAGAAGAA 
GAAATAGAAG CTGAGCGTGC AGCTCGTGCC AAGGCAGAGA AGCAGCGTTC CGACCTCTCC 
AGGGAACTTG AGGAGATCAG TGAGCGCCTG GAGGAAGCTG GAGGTGCCAC CTCTGCTCAG 
ATTGAGATGA ACAAGAAGCG CGAGGCAGAA TTCCAGAAGA TGAGACGTGA CCTTGAAGAG 
GCCACTCTGC AACACGAAGC CACCGCTGCT GCTCTCAGAA AGAAGCAGGC CGACAGCGTG 
GCTGAACTCG GGGAACAAAT CGATAACCTG CAAAGAGTCA AGCAGAAGTT AGAAAAGGAG 
AAAAGTGAGC TAAAGATGGA GATTGATGAC CTGGCCAGCA ACATGGAAAA CCTATCCAAA 
GCCAAGGCCA ATTTTGAGAA GATTTGCCGC ACCCTTGAAG ATCAGCTGAG TGAACTCAAG 
ACAAAGGATG AGGAACACCA GAGGCAGATT AACGACCTCA CTGCCCAAAG GGCCCGACTG 
CAGACAGAAA ATGGTGAATA TGCACGACAG TTGGAGGAAA GAGATTCTTT GATCTCCCAG 
CTTTCTCGAG GCAAACAGGC ATTTACTCAA CAGATTGAAG AGCTCAAGAG GCAGCTGGAA 
GAGGAAATCA AGGCCAAGAA TGCCCTTGCC CATGGTCTGC AGTCAGCCCG CCATGATTGC 
GACTTGCTTA GAGAACAATA TGAGGAAGAG CAAGAGGCCA AGGGTGAGCT TCAGCGCTCG 
TTGTCCAAAG CCAATGGTGA AGTTGCCCAG TGGAGGACAA AGTACGAGAC AGACGCTATT 
CAGCGTACAG AAGAACTAGA GGATGCCAAG AAGAAACTGG CTCAGCGTCT CCAGGAAGCA 
GAAGAACACA TTGAAGCTGT GAACTCAAAG TGCGCTTCTC TCGAAAAGAC CAAACAGCGA 
CTTCAGAATG AAGTTGAAGA CCTTATGGTT GATGTAGAAC GGTCAAACAG TGCTTGTGCT 
GCTCTAGATA AGAAACAGAA GAACTTCGAT AAGATTCTGG CAGAATGGAA GCAGAAGTTT 
GAAGAGAGCC AGGCTGAACT GGAAGCCTCT CAGAAGGAGT CCCGGTCTCT CAGCACAGAG 
TTGTTCAAGA TGAAGAATGC CTATGAGGAA TCCCTGGATC ACCTCGAGAC AACCAAACGA 
GAGAACAAGA ACTTGCAGCA GGAGATTTCC GATCTTACTG AACAAGTTTC TGAAGTTTCC 
AAGACACTGC ACGAGGTGGA AAAAGCCAAG AAAATTATTG AGCAAGAAAA GACTGAAATT 
CAGTCTGCTC TAGAAGAAGC TGAGGCATCT CTGGAACACG AGGAAGGCAA GATACTGAGA 
ATCCAGCTGG AACTGAATCA AGTGAAATCA GAAATTGACA GAAAGATTGC AGAGAAGGAT 
GAGGAAATCG ATCAGTTGAA AAGGAACCAC GTCAGGGTGG TTGAGTCCAT GCAGAGCACA 
CTGGATGCTG AAATCAGAAG CAGGAACGAT GCTCTAAGGT TGAAGAAGAA AATGGAAGGA 
GATCTCAATG AAATGGAGAT CCAGTTGAGC CATGCCAATC GACAAGCTTC TGAGTCTCAG 
AAGCAGCTGA GAAATGTCCA GGGATTGCTC AAGGATGTTC AGATCCACTT GGACGACTCC 
CTGAGAGCCC AGGAAGATCT TAAGGAGCAA TTGGCTATGG TTGAACGCAG AGCTAATCTG 
ATGCAGGCTG AGATAGAAGA GATGAGGGCT GCTCTGGAAC AAACAGAACG GAGCAGGAAA 
GTCGCAGAAC AAGAACTACT GGATGCTAGT GAGCGTGTCC AGCTCCTGCA CTCACAGAAC 
ACAAGCTTGA TCAACACCAA GAAGAAGATG GAGACAGACA TCACTCAGCT ACAGACTGAG 
GTTGAAGAGG CCATACAAGA AGCCCGCAAT GCAGAAGAAA AGGCCAAAAA GGCCATAACA 
GATGCTGCCA TGATGGCAGA AGAGCTTAAA AAGGAACAGG ACACCAGTGC TCACTTGGAA 
AGGATGAAGA AGAACCTCGA TCAAACAGTG AAGGATCTGC AGCACCGTTT GGATGAAGCT 
GAACAACTGG CAATGAAAGG AGGAAAGAAA CAAATCCAGA AACTGGAGGC TAGGGTTCGG 
GAGCTTGAAA ATGAAGTGGA TGTAGAACAG AAACGCAGCA CAGAGGCCAT TAAAGGCGTC 
CGAAAATACG AACGGCGGGT TAAAGAACTA TCATACCAGT CTGAGGAGGA TAAGAAGAAT 
ATCCTGAGAC TTCAAGACCT GGTGGACAAA CTCCAAATGA AGGTGAAGGC CTACAAGAGG 
CAGACAGAGG AAATTGAGGA ACAGTCCAAT ATCAACCTCA CCAAATACCG GAAGATCCAA 
CATGAACTTG AGGAAGCCGA GGAACGTGCC GATATTGCAG AATCCCAGGT GAACAAGCTC 
CGCGCCAAGA CCCGAGAGGT CACCAAGATC ACGAAGAGCG AAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012809632.1
CDS	105..5912
Translation	MSSDAEMAVFGPAAHFLRKSEKERIEAQNKPFDARTSMFVVDTKESYVKGTLQSKEGGKATVKTEAGQTVTVKDDQIFPMNPPKFDKIEDMAMMTHLNEPSVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPEVVNAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAAAPDKKKEEVAKTKGTLEDQIIQANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQIMSNKKPELIEMLLITTNPYDFPFVSQGEISVASIDDTEELMATDSAIDILGFNADEKVSIYKLTGAVMHYGNMRFKQKQREEQAEPDGTEVADKAAYLMCLNSADLLKALCYPRVKVGNEYVTKGQTVQQVYNSVGALGKAVYEKMFLWMVTRINEMLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDQHLGKSNNFQKPKPAKGKAEAHFSLVHYAGTVDYNISGWLEKNKDPLNDTVIGLFQKSSMKTLAYLFSSYAASEAEGAKKGGKKKGSSFQTVSALFRENLNKLMSNLRTTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYGDFKQRYKILNASAIPEGQFIDSKKASEKLLGSIDVDHTQYRFGHTKVFFKAGLLGTLEEMRDDRLAHVITRTQAMCRGYLMRVEFKKMMERREAVFTIQYNIRSFMNVKHWPWMKLYFKIKPLLKSAESEKEMQNMKEEFEKTKELLAKSEAKRKELEEKMVKVLQEKNDLQLQVQSESENLTDAEERCDQLIKSKIQLEAKMKELNERLEDEEESNAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAVLDETIVKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKAKLEQQVDDLEGSLEQEKKLRMDLERSKRKLEGDLKLAQETIMDLENDRQQLDEKLKKKEFEMSQYASKIEDEQALGAQLQKKIKELQARIEELEEEIEAERAARAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKQADSVAELGEQIDNLQRVKQKLEKEKSELKMEIDDLASNMENLSKAKANFEKICRTLEDQLSELKTKDEEHQRQINDLTAQRARLQTENGEYARQLEERDSLISQLSRGKQAFTQQIEELKRQLEEEIKAKNALAHGLQSARHDCDLLREQYEEEQEAKGELQRSLSKANGEVAQWRTKYETDAIQRTEELEDAKKKLAQRLQEAEEHIEAVNSKCASLEKTKQRLQNEVEDLMVDVERSNSACAALDKKQKNFDKILAEWKQKFEESQAELEASQKESRSLSTELFKMKNAYEESLDHLETTKRENKNLQQEISDLTEQVSEVSKTLHEVEKAKKIIEQEKTEIQSALEEAEASLEHEEGKILRIQLELNQVKSEIDRKIAEKDEEIDQLKRNHVRVVESMQSTLDAEIRSRNDALRLKKKMEGDLNEMEIQLSHANRQASESQKQLRNVQGLLKDVQIHLDDSLRAQEDLKEQLAMVERRANLMQAEIEEMRAALEQTERSRKVAEQELLDASERVQLLHSQNTSLINTKKKMETDITQLQTEVEEAIQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLDQTVKDLQHRLDEAEQLAMKGGKKQIQKLEARVRELENEVDVEQKRSTEAIKGVRKYERRVKELSYQSEEDKKNILRLQDLVDKLQMKVKAYKRQTEEIEEQSNINLTKYRKIQHELEEAEERADIAESQVNKLRAKTREVTKITKSEE

Target ORF information:

RefSeq Version	XM_012954178.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin, heavy chain 3, skeletal muscle, embryonic (myh3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012954178.2

ORF Insert Sequence:

ATGAGTTCGG ACGCGGAGAT GGCCGTTTTC GGGCCAGCAG CCCATTTTCT CAGAAAAAGT 
GAGAAAGAGC GGATCGAGGC GCAGAACAAA CCATTCGACG CAAGGACCTC CATGTTTGTG 
GTGGATACCA AGGAATCCTA CGTGAAAGGG ACCCTCCAAA GCAAGGAGGG CGGGAAGGCT 
ACTGTAAAGA CAGAAGCGGG ACAGACTGTA ACAGTGAAGG ATGATCAGAT CTTCCCCATG 
AACCCACCAA AGTTCGATAA AATTGAAGAC ATGGCCATGA TGACCCACTT GAACGAACCA 
TCTGTCCTGT ATAACCTCAA AGAGCGTTAC GCAGCCTGGA TGATCTACAC CTATTCTGGG 
CTCTTCTGCG TCACTGTCAA CCCCTACAAG TGGCTTCCCG TGTACAACCC AGAGGTGGTC 
AATGCCTACC GTGGGAAGAA GCGTCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAT 
AACGCCTATC AGTTCATGTT AACTGATCGT GAGAACCAGT CTATCCTGAT CACTGGAGAA 
TCCGGTGCCG GGAAGACCGT AAACACCAAG CGTGTCATTC AGTATTTTGC AACAATTGCA 
GCCGCCCCCG ACAAGAAAAA AGAAGAGGTG GCAAAGACCA AGGGGACCCT GGAGGATCAA 
ATCATTCAGG CCAACCCGCT GCTGGAAGCC TTCGGCAATG CCAAGACCGT CAGGAACGAC 
AACTCTTCCC GCTTTGGTAA ATTCATCAGA ATCCATTTCG GCACGACTGG GAAACTCGCC 
TCTGCTGATA TTGAAACATA CCTGCTGGAG AAGTCCAGAG TTACCTTCCA GCTAAAGGCC 
GAGAGGAGCT ATCACATTTT CTACCAAATC ATGTCCAACA AAAAGCCCGA ACTGATTGAA 
ATGCTCCTCA TCACCACCAA CCCCTACGAC TTTCCATTCG TCAGCCAAGG GGAGATCTCT 
GTGGCCAGTA TCGACGATAC GGAGGAACTC ATGGCCACTG ATAGCGCCAT CGATATCCTG 
GGCTTCAATG CGGATGAGAA AGTTTCTATC TATAAACTGA CCGGTGCCGT CATGCACTAC 
GGGAACATGA GATTCAAGCA GAAACAGCGA GAGGAGCAGG CCGAGCCGGA CGGCACAGAA 
GTGGCCGACA AAGCTGCCTA TTTGATGTGC CTGAACTCAG CAGATCTGCT GAAGGCTCTG 
TGCTACCCAA GGGTCAAGGT CGGCAATGAA TATGTCACCA AGGGGCAAAC TGTCCAGCAG 
GTGTACAACT CAGTCGGCGC TCTGGGCAAA GCTGTTTACG AGAAGATGTT CTTGTGGATG 
GTCACGCGTA TCAATGAAAT GTTGGACACC AAGCAGCCAA GGCAGTATTT CATCGGTGTG 
CTGGACATCG CTGGCTTTGA GATCTTTGAT TTCAACAGCT TGGAGCAGCT CTGCATCAAC 
TTCACCAACG AGAAACTGCA ACAGTTTTTC AACCACCACA TGTTTGTCCT GGAGCAAGAG 
GAGTACAAGA AGGAGGGGAT CGAGTGGGAG TTCATTGACT TCGGCATGGA CTTGGCCGCC 
TGCATTGAGC TTATTGAGAA GCCAATGGGC ATTTTCTCCA TCCTTGAAGA GGAGTGCATG 
TTCCCCAAGG CAACAGACAC TTCATTCAAG AACAAGTTGT ACGACCAACA TCTGGGCAAG 
TCCAACAACT TCCAGAAGCC CAAACCGGCC AAAGGCAAAG CCGAGGCCCA CTTCTCTCTT 
GTCCACTATG CCGGGACGGT AGATTATAAC ATCAGTGGTT GGCTTGAAAA GAACAAGGAC 
CCACTGAATG ACACTGTCAT TGGCCTCTTC CAGAAGTCTT CAATGAAGAC ACTGGCGTAT 
CTGTTCTCCA GCTATGCTGC TTCTGAAGCA GAGGGTGCAA AGAAAGGTGG TAAGAAGAAG 
GGGTCTTCTT TCCAGACCGT GTCTGCTCTT TTTAGGGAAA ATCTGAATAA GCTAATGAGC 
AATCTGAGGA CCACCCATCC TCACTTTGTC CGTTGCATTA TTCCCAATGA GACCAAAACT 
CCAGGGGCAA TGGAACATGA ACTTGTATTG CATCAGCTCA GGTGTAATGG GGTGCTGGAG 
GGGATCAGGA TTTGCAGAAA GGGATTCCCA AGCAGGATTC TCTACGGTGA CTTCAAGCAA 
AGATACAAAA TTTTAAATGC AAGTGCCATC CCAGAGGGGC AGTTTATTGA CAGCAAGAAG 
GCCTCTGAAA AACTTCTTGG ATCCATTGAT GTTGATCATA CCCAGTACAG ATTTGGCCAC 
ACTAAGGTGT TCTTCAAAGC TGGTCTTCTG GGTACCCTGG AAGAGATGCG AGACGATCGC 
TTGGCCCATG TAATCACACG TACCCAGGCT ATGTGCAGAG GGTACCTCAT GAGGGTGGAG 
TTTAAGAAAA TGATGGAGAG AAGGGAAGCT GTGTTCACTA TTCAATACAA TATCCGGTCC 
TTCATGAATG TCAAGCATTG GCCATGGATG AAGCTTTATT TCAAGATCAA GCCTCTCCTG 
AAGAGTGCCG AGTCCGAGAA AGAGATGCAG AACATGAAGG AAGAGTTTGA GAAGACCAAG 
GAGCTGCTGG CCAAGTCAGA AGCTAAACGT AAGGAGCTGG AAGAGAAGAT GGTTAAAGTT 
CTCCAAGAGA AGAATGACCT GCAACTTCAA GTCCAGTCTG AATCAGAAAA TCTCACAGAT 
GCCGAGGAAA GGTGCGATCA GTTAATCAAA TCCAAAATCC AGTTAGAAGC CAAAATGAAA 
GAGCTGAATG AGAGGCTGGA AGACGAGGAG GAATCCAACG CTGAACTGAC AGCCAAGAAG 
AGGAAACTTG AAGATGAATG CTCAGAACTG AAGAAAGATA TTGACGATCT GGAGTTAACG 
CTGGCCAAAG TTGAAAAAGA GAAACACGCA ACAGAGAACA AGGTTAAGAA CCTCACTGAA 
GAAATGGCAG TTTTGGATGA GACCATCGTT AAGCTTACCA AAGAGAAAAA AGCCCTTCAA 
GAAGCTCACC AGCAGACCCT TGATGACCTC CAGGCGGAGG AAGACAAAGT CAACACTCTG 
ACCAAAGCCA AGGCCAAGCT TGAGCAGCAA GTCGACGATC TTGAAGGGTC TTTGGAACAA 
GAGAAGAAAC TGCGCATGGA CCTTGAAAGA TCTAAGAGAA AGCTAGAGGG TGATCTTAAA 
CTTGCCCAGG AAACCATCAT GGACCTGGAA AACGATAGGC AGCAACTGGA TGAAAAGCTA 
AAAAAGAAAG AATTTGAAAT GAGCCAGTAC GCAAGCAAAA TTGAGGATGA GCAGGCTCTT 
GGTGCTCAAC TGCAGAAAAA GATTAAGGAA TTGCAGGCCC GCATTGAGGA GCTAGAAGAA 
GAAATAGAAG CTGAGCGTGC AGCTCGTGCC AAGGCAGAGA AGCAGCGTTC CGACCTCTCC 
AGGGAACTTG AGGAGATCAG TGAGCGCCTG GAGGAAGCTG GAGGTGCCAC CTCTGCTCAG 
ATTGAGATGA ACAAGAAGCG CGAGGCAGAA TTCCAGAAGA TGAGACGTGA CCTTGAAGAG 
GCCACTCTGC AACACGAAGC CACCGCTGCT GCTCTCAGAA AGAAGCAGGC CGACAGCGTG 
GCTGAACTCG GGGAACAAAT CGATAACCTG CAAAGAGTCA AGCAGAAGTT AGAAAAGGAG 
AAAAGTGAGC TAAAGATGGA GATTGATGAC CTGGCCAGCA ACATGGAAAA CCTATCCAAA 
GCCAAGGCCA ATTTTGAGAA GATTTGCCGC ACCCTTGAAG ATCAGCTGAG TGAACTCAAG 
ACAAAGGATG AGGAACACCA GAGGCAGATT AACGACCTCA CTGCCCAAAG GGCCCGACTG 
CAGACAGAAA ATGGTGAATA TGCACGACAG TTGGAGGAAA GAGATTCTTT GATCTCCCAG 
CTTTCTCGAG GCAAACAGGC ATTTACTCAA CAGATTGAAG AGCTCAAGAG GCAGCTGGAA 
GAGGAAATCA AGGCCAAGAA TGCCCTTGCC CATGGTCTGC AGTCAGCCCG CCATGATTGC 
GACTTGCTTA GAGAACAATA TGAGGAAGAG CAAGAGGCCA AGGGTGAGCT TCAGCGCTCG 
TTGTCCAAAG CCAATGGTGA AGTTGCCCAG TGGAGGACAA AGTACGAGAC AGACGCTATT 
CAGCGTACAG AAGAACTAGA GGATGCCAAG AAGAAACTGG CTCAGCGTCT CCAGGAAGCA 
GAAGAACACA TTGAAGCTGT GAACTCAAAG TGCGCTTCTC TCGAAAAGAC CAAACAGCGA 
CTTCAGAATG AAGTTGAAGA CCTTATGGTT GATGTAGAAC GGTCAAACAG TGCTTGTGCT 
GCTCTAGATA AGAAACAGAA GAACTTCGAT AAGATTCTGG CAGAATGGAA GCAGAAGTTT 
GAAGAGAGCC AGGCTGAACT GGAAGCCTCT CAGAAGGAGT CCCGGTCTCT CAGCACAGAG 
TTGTTCAAGA TGAAGAATGC CTATGAGGAA TCCCTGGATC ACCTCGAGAC AACCAAACGA 
GAGAACAAGA ACTTGCAGCA GGAGATTTCC GATCTTACTG AACAAGTTTC TGAAGTTTCC 
AAGACACTGC ACGAGGTGGA AAAAGCCAAG AAAATTATTG AGCAAGAAAA GACTGAAATT 
CAGTCTGCTC TAGAAGAAGC TGAGGCATCT CTGGAACACG AGGAAGGCAA GATACTGAGA 
ATCCAGCTGG AACTGAATCA AGTGAAATCA GAAATTGACA GAAAGATTGC AGAGAAGGAT 
GAGGAAATCG ATCAGTTGAA AAGGAACCAC GTCAGGGTGG TTGAGTCCAT GCAGAGCACA 
CTGGATGCTG AAATCAGAAG CAGGAACGAT GCTCTAAGGT TGAAGAAGAA AATGGAAGGA 
GATCTCAATG AAATGGAGAT CCAGTTGAGC CATGCCAATC GACAAGCTTC TGAGTCTCAG 
AAGCAGCTGA GAAATGTCCA GGGATTGCTC AAGGATGTTC AGATCCACTT GGACGACTCC 
CTGAGAGCCC AGGAAGATCT TAAGGAGCAA TTGGCTATGG TTGAACGCAG AGCTAATCTG 
ATGCAGGCTG AGATAGAAGA GATGAGGGCT GCTCTGGAAC AAACAGAACG GAGCAGGAAA 
GTCGCAGAAC AAGAACTACT GGATGCTAGT GAGCGTGTCC AGCTCCTGCA CTCACAGAAC 
ACAAGCTTGA TCAACACCAA GAAGAAGATG GAGACAGACA TCACTCAGCT ACAGACTGAG 
GTTGAAGAGG CCATACAAGA AGCCCGCAAT GCAGAAGAAA AGGCCAAAAA GGCCATAACA 
GATGCTGCCA TGATGGCAGA AGAGCTTAAA AAGGAACAGG ACACCAGTGC TCACTTGGAA 
AGGATGAAGA AGAACCTCGA TCAAACAGTG AAGGATCTGC AGCACCGTTT GGATGAAGCT 
GAACAACTGG CAATGAAAGG AGGAAAGAAA CAAATCCAGA AACTGGAGGC TAGGGTTCGG 
GAGCTTGAAA ATGAAGTGGA TGTAGAACAG AAACGCAGCA CAGAGGCCAT TAAAGGCGTC 
CGAAAATACG AACGGCGGGT TAAAGAACTA TCATACCAGT CTGAGGAGGA TAAGAAGAAT 
ATCCTGAGAC TTCAAGACCT GGTGGACAAA CTCCAAATGA AGGTGAAGGC CTACAAGAGG 
CAGACAGAGG AAATTGAGGA ACAGTCCAAT ATCAACCTCA CCAAATACCG GAAGATCCAA 
CATGAACTTG AGGAAGCCGA GGAACGTGCC GATATTGCAG AATCCCAGGT GAACAAGCTC 
CGCGCCAAGA CCCGAGAGGT CACCAAGATC ACGAAGAGCG AAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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myh3 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

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Target ORF information:

ORF Insert Sequence:

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