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alg8 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Summary

Gene Symbol alg8
Entrez Gene ID 548388
Full Name ALG8, alpha-1,3-glucosyltransferase
General protein information
Preferred Names

ALG8, alpha-1,3-glucosyltransferase

Names

probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase
asparagine-linked glycosylation 8 homolog (yeast, alpha-1,3-glucosyltransferase)
asparagine-linked glycosylation 8, alpha-1,3-glucosyltransferase homolog
Gene Type protein-coding
Organism Xenopus tropicalis(tropical clawed frog)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001083351.1 NP_001076820.1 probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase
XM_012956885.2 XP_012812339.1 probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase isoform X1

Pathways from BioSystems


Homologies


Pan troglodytes (chimpanzee) ALG8 XP_003313272.1
Canis lupus familiaris (dog) ALG8 XP_533998.2
Mus musculus (house mouse) Alg8 NP_950200.2
Danio rerio (zebrafish) alg8 NP_001017647.1
Drosophila melanogaster (fruit fly) CG4542 NP_572355.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP003928 XP_318380.5
Schizosaccharomyces pombe (fission yeast) alg8 NP_594599.1
Saccharomyces cerevisiae (baker's yeast) ALG8 NP_014710.1
Neurospora crassa NCU00163 XP_956676.1
Arabidopsis thaliana (thale cress) AT2G44660 NP_181994.5
Xenopus tropicalis (tropical clawed frog) alg8 NP_001076820.1
Gallus gallus (chicken) ALG8 XP_004938993.1
Kluyveromyces lactis KLLA0F16621g XP_455827.1
Eremothecium gossypii AGOS_ADR210C NP_984306.2
Homo sapiens (human) ALG8 NP_076984.2
Rattus norvegicus (Norway rat) Alg8 NP_001029299.1
Bos taurus (cattle) ALG8 NP_001069593.1
Magnaporthe oryzae (rice blast fungus) MGG_05686 XP_003710585.1
Oryza sativa (rice) Os02g0688500 NP_001047779.1

Gene Ontology


Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following alg8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the alg8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa24325 NM_001083351.1
Latest version!		 Xenopus tropicalis ALG8, alpha-1,3-glucosyltransferase (alg8), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $342.30
$489.00
OXa27345 XM_012956885.2
Latest version!		 Xenopus tropicalis ALG8, alpha-1,3-glucosyltransferase (alg8), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $258.30
$369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone 40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OXa24325
Clone ID Related Accession (Same CDS sequence) NM_001083351.1
Accession Version NM_001083351.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1629bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-10-27
Organism Xenopus tropicalis(tropical clawed frog)
Product probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC135531.1. ##Evidence-Data-START## Transcript exon combination :: BC135531.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1025683, SAMEA1025684 [ECO:0000350] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGGCGGCTC CCAAAGGTGC TAACGTGGGC TCCCGCAATA GCAGCACCGG CTCCAAGAGT 
AAAGAGAGCG GGGATGGGGG CTGGTTTGTT ACCCTGGCTA TCGCCGTGTC CCTGCTGAAA 
TGCCTTCTCC TTCCGACCTA CCATTCTACA GATTTTGAGG TGCACAGAAA CTGGTTGGCA 
ATAACCCACA GCCTGCCTGT ATCTAAGTGG TACTATGAGG CAACCTCGGA GTGGACATTG 
GACTACCCCC CTTTCTTTGC ATGGTTTGAG CATCTGCTCG CCAAAGTGGC GCAGTACGTT 
GATCCGGAGA TGCTGAAGGT GGAGAATTTA AACTATGCCA GCCAGGAGAC CGTCCTCTTC 
CAGAGGCTTT CTGTTATTAT CACAGATATC CTCTTCATCT ACGCTGCCAG CCAATGCTGT 
AAGTGTGTGA ATGGGCGGAA GGATCGCAGG GATCTTATAC AGAAGCCGGC CTTTGTCCTG 
GCGGTGCTGC TGCTATGGAA CTTCGGCCTG CTGATTGTGG ATCATATTCA CTTCCAGTAC 
AATGGGTTCC TCTCGGGCAT CATGCTCCTC TCCATTGCAC GCTTGTATCA GAAGAGAGTT 
ATGGAAAGCG CCTTCCTTTT TGCCGTCCTT CTCAATTTCA AGCACATCTA CCTGTACATT 
GCTCCGGCCT ATGGGATGTA CATGCTGCGT TCCTACTGTT TCACAGGGAA TAATCCAGAC 
GGATCCGTGC GCTGGAGAAG TTTCAGCTGC CTGCGACTCC TCTCTCTCGG CCTGACGGTC 
TGCTCGGTGT TTGCACTTTC CTTTGGGCCC TTTGTCTACT TGGGCCAACT CCCTCAGGTG 
CTGTCTCGGC TTTTCCCTTT CAAACGAGGC CTCTGCCATG CATACTGGGC ACCCAATTTC 
TGGGCACTGT ACAACATGGT GGATAAAGCT CTGTCCATTA TAGGTGTGAA GCTGCAGCTG 
CTGGACCCAG CCACTATCCG TACTGGGACA ATGACGAGGG GATTGGTGCA GGAGTTTGAG 
CACAGTGTTC TTCCCTCAGT TACACCAATG GCAACGCTAG TCTGTACCCT CGTCTCCATA 
CTGCCCTCTG TGCTCCGACT ATGGCGCAAG CCTCAAGGAC CCACTGGTTT CCTTCGCTGC 
CTAATCCTTT GCGCACTGGG ATCCTTCATG TTCGGTTGGC ACGTGCATGA GAAAGCCATC 
CTCCTGGCAA TCCTCCCACT AAGCATCTTG TCTGTGACCA GTGCAAAGGA TGCTGGGATA 
TTTTTGGTCC TGGCCACGAC GGGCCACTTC TCGCTTTTCC CTCTGCTCTT CACAGCGCCA 
GAACTGCCCA TTAAGGTGCT GCTGATGTTG ATCTTCACTG TATTTAGTGT CTCGGCCCTG 
AGAGCCATGT TTCAAAGAGA GAGGGCGCTG CTGAGCTGGG TGGAAACCCT ATACCTGTGC 
GGGTTGGTGC CCCTGGGGGC CTTCTGTGAA GTTGTGTACC CTCTGACCTC CTGGCACCAA 
CAGCTCCCGT TTGTGCCCCT GCTCCTCTCC TCTGGGTACT GCGCCCTGGG GGTTACCTAC 
TCTTGGGCCA GACTTTACAT TTCAGCGCTT TCTGACTTGC CATCCGCCAA GCAAAAGAAG 
AACCAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001076820.1
CDS5..1633
Translation

Target ORF information:

RefSeq Version NM_001083351.1
Organism Xenopus tropicalis(tropical clawed frog)
Definition Xenopus tropicalis ALG8, alpha-1,3-glucosyltransferase (alg8), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001083351.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGGCGGCTC CCAAAGGTGC TAACGTGGGC TCCCGCAATA GCAGCACCGG CTCCAAGAGT 
AAAGAGAGCG GGGATGGGGG CTGGTTTGTT ACCCTGGCTA TCGCCGTGTC CCTGCTGAAA 
TGCCTTCTCC TTCCGACCTA CCATTCTACA GATTTTGAGG TGCACAGAAA CTGGTTGGCA 
ATAACCCACA GCCTGCCTGT ATCTAAGTGG TACTATGAGG CAACCTCGGA GTGGACATTG 
GACTACCCCC CTTTCTTTGC ATGGTTTGAG CATCTGCTCG CCAAAGTGGC GCAGTACGTT 
GATCCGGAGA TGCTGAAGGT GGAGAATTTA AACTATGCCA GCCAGGAGAC CGTCCTCTTC 
CAGAGGCTTT CTGTTATTAT CACAGATATC CTCTTCATCT ACGCTGCCAG CCAATGCTGT 
AAGTGTGTGA ATGGGCGGAA GGATCGCAGG GATCTTATAC AGAAGCCGGC CTTTGTCCTG 
GCGGTGCTGC TGCTATGGAA CTTCGGCCTG CTGATTGTGG ATCATATTCA CTTCCAGTAC 
AATGGGTTCC TCTCGGGCAT CATGCTCCTC TCCATTGCAC GCTTGTATCA GAAGAGAGTT 
ATGGAAAGCG CCTTCCTTTT TGCCGTCCTT CTCAATTTCA AGCACATCTA CCTGTACATT 
GCTCCGGCCT ATGGGATGTA CATGCTGCGT TCCTACTGTT TCACAGGGAA TAATCCAGAC 
GGATCCGTGC GCTGGAGAAG TTTCAGCTGC CTGCGACTCC TCTCTCTCGG CCTGACGGTC 
TGCTCGGTGT TTGCACTTTC CTTTGGGCCC TTTGTCTACT TGGGCCAACT CCCTCAGGTG 
CTGTCTCGGC TTTTCCCTTT CAAACGAGGC CTCTGCCATG CATACTGGGC ACCCAATTTC 
TGGGCACTGT ACAACATGGT GGATAAAGCT CTGTCCATTA TAGGTGTGAA GCTGCAGCTG 
CTGGACCCAG CCACTATCCG TACTGGGACA ATGACGAGGG GATTGGTGCA GGAGTTTGAG 
CACAGTGTTC TTCCCTCAGT TACACCAATG GCAACGCTAG TCTGTACCCT CGTCTCCATA 
CTGCCCTCTG TGCTCCGACT ATGGCGCAAG CCTCAAGGAC CCACTGGTTT CCTTCGCTGC 
CTAATCCTTT GCGCACTGGG ATCCTTCATG TTCGGTTGGC ACGTGCATGA GAAAGCCATC 
CTCCTGGCAA TCCTCCCACT AAGCATCTTG TCTGTGACCA GTGCAAAGGA TGCTGGGATA 
TTTTTGGTCC TGGCCACGAC GGGCCACTTC TCGCTTTTCC CTCTGCTCTT CACAGCGCCA 
GAACTGCCCA TTAAGGTGCT GCTGATGTTG ATCTTCACTG TATTTAGTGT CTCGGCCCTG 
AGAGCCATGT TTCAAAGAGA GAGGGCGCTG CTGAGCTGGG TGGAAACCCT ATACCTGTGC 
GGGTTGGTGC CCCTGGGGGC CTTCTGTGAA GTTGTGTACC CTCTGACCTC CTGGCACCAA 
CAGCTCCCGT TTGTGCCCCT GCTCCTCTCC TCTGGGTACT GCGCCCTGGG GGTTACCTAC 
TCTTGGGCCA GACTTTACAT TTCAGCGCTT TCTGACTTGC CATCCGCCAA GCAAAAGAAG 
AACCAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OXa27345
Clone ID Related Accession (Same CDS sequence) XM_012956885.2
Accession Version XM_012956885.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1404bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-09-07
Organism Xenopus tropicalis(tropical clawed frog)
Product probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012956885.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCGGCTC CCAAAGGTGC TAACGTGGGC TCCCGCAATA GTAGCACCGG CCCCGGCTCC 
AAGAGCAAAG AGAGCGGGGA TGGGGGCTGG TTTGTAACCC TGGCTATCGC CGTGTCCCTG 
CTGAAATGCC TTCTCCTTCC GACCTACCAT TCTACAGATT TTGAGGTGCA CAGAAACTGG 
TTGGCAATAA CCCACAGCCT GCCTGTATCT AAGTGGTACT ATGAGGCCAC CTCGGAGTGG 
ACATTGGACT ACCCCCCTTT CTTTGCATGG TTTGAGCATC TGCTCGCCAA AGTGGCGCAG 
TACGTTGATC CGGAGATGCT GAAGGTGGAG AATTTAAACT ATGCCAGCCA GGAGACCGTC 
CTCTTCCAGA GGCTTTCTGT TATTATCACA GATATCCTCT TCATCTACGC TGCCAGCCAA 
TGCTGTAAGT GTGTGAATGG GCGGAAGGAT CGCAGGGATC TTATACAGAA GCCGGCCTTT 
GTCCTGGCGG TGCTGCTGCT ATGGAACTTC GGCCTGCTGA TTGTGGATCA TATTCACTTC 
CAGTACAATG GGTTCCTCTC GGGCATCATG CTCCTTTCCA TTGCACGCTT GTATCAGAAG 
AGAGTAATGG AAAGCGCCTT CCTTTTTGCC GTCCTTCTCA ATTTCAAGCA CATCTACCTG 
TACATTGCCC CGGCCTATGG GATGTACATG CTGCGTTCCT ACTGTTTCAC AGGGAATAAT 
CCAGACGGAT CCGTGCGCTG GAGAAGTTTC AGCTGCCTGC GACTCCTCTC TCTCGGCCTG 
ACGGTCTGCT CGGTGTTTGC ACTTTCCTTT GGGCCCTTTG TCTACTTGGG CCAACTCCCT 
CAGGTGCTGT CTCGGCTTTT CCCTTTCAAA CGAGGCCTCT GCCATGCATA CTGGGCACCC 
AATTTCTGGG CACTGTACAA CGTGGTGGAT AAAGCTCTGT CCATTATAGG TGTGAAGCTG 
CAGCTGCTGG ACCCAGCCAC TATCCGTACG GGGACAATGA CGAGGGGATT GGTGCAGGAG 
TTTGAGCACA GTGTTCTTCC CTCAGTTACA CCAATGGCAA CGCTAGTCTG TACCCTCATC 
TCCATACTGC CCTCTGTGCT CCGACTATGG CGCAAGCCTC AAGGACCCAC TGGTTTCCTT 
CGCTGCCTAA TCCTTTGCGC ACTGGGATCC TTCATGTTCG GTTGGCACGT TCATGAGAAA 
GCCATCCTCC TGGCAATTCT CCCACTAAGC ATCTTGTCTG TGACCAGTGC AAAGGATGCT 
GGGATATTTT TGGTCCTGGC CACGACGGGC CACTTCTCGC TTTTCCCTCT GCTCTTCACA 
GCGCCAGAAC TGCCCATTAA GGTGCTGCTG ATGTTGATCT TCACTGTATT TAGTGTCTCT 
GCCCTGAGAG CCATGTTTCA GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_012812339.1
CDS79..1482
Translation

Target ORF information:

RefSeq Version XM_012956885.2
Organism Xenopus tropicalis(tropical clawed frog)
Definition Xenopus tropicalis ALG8, alpha-1,3-glucosyltransferase (alg8), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_012956885.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCGGCTC CCAAAGGTGC TAACGTGGGC TCCCGCAATA GTAGCACCGG CCCCGGCTCC 
AAGAGCAAAG AGAGCGGGGA TGGGGGCTGG TTTGTAACCC TGGCTATCGC CGTGTCCCTG 
CTGAAATGCC TTCTCCTTCC GACCTACCAT TCTACAGATT TTGAGGTGCA CAGAAACTGG 
TTGGCAATAA CCCACAGCCT GCCTGTATCT AAGTGGTACT ATGAGGCCAC CTCGGAGTGG 
ACATTGGACT ACCCCCCTTT CTTTGCATGG TTTGAGCATC TGCTCGCCAA AGTGGCGCAG 
TACGTTGATC CGGAGATGCT GAAGGTGGAG AATTTAAACT ATGCCAGCCA GGAGACCGTC 
CTCTTCCAGA GGCTTTCTGT TATTATCACA GATATCCTCT TCATCTACGC TGCCAGCCAA 
TGCTGTAAGT GTGTGAATGG GCGGAAGGAT CGCAGGGATC TTATACAGAA GCCGGCCTTT 
GTCCTGGCGG TGCTGCTGCT ATGGAACTTC GGCCTGCTGA TTGTGGATCA TATTCACTTC 
CAGTACAATG GGTTCCTCTC GGGCATCATG CTCCTTTCCA TTGCACGCTT GTATCAGAAG 
AGAGTAATGG AAAGCGCCTT CCTTTTTGCC GTCCTTCTCA ATTTCAAGCA CATCTACCTG 
TACATTGCCC CGGCCTATGG GATGTACATG CTGCGTTCCT ACTGTTTCAC AGGGAATAAT 
CCAGACGGAT CCGTGCGCTG GAGAAGTTTC AGCTGCCTGC GACTCCTCTC TCTCGGCCTG 
ACGGTCTGCT CGGTGTTTGC ACTTTCCTTT GGGCCCTTTG TCTACTTGGG CCAACTCCCT 
CAGGTGCTGT CTCGGCTTTT CCCTTTCAAA CGAGGCCTCT GCCATGCATA CTGGGCACCC 
AATTTCTGGG CACTGTACAA CGTGGTGGAT AAAGCTCTGT CCATTATAGG TGTGAAGCTG 
CAGCTGCTGG ACCCAGCCAC TATCCGTACG GGGACAATGA CGAGGGGATT GGTGCAGGAG 
TTTGAGCACA GTGTTCTTCC CTCAGTTACA CCAATGGCAA CGCTAGTCTG TACCCTCATC 
TCCATACTGC CCTCTGTGCT CCGACTATGG CGCAAGCCTC AAGGACCCAC TGGTTTCCTT 
CGCTGCCTAA TCCTTTGCGC ACTGGGATCC TTCATGTTCG GTTGGCACGT TCATGAGAAA 
GCCATCCTCC TGGCAATTCT CCCACTAAGC ATCTTGTCTG TGACCAGTGC AAAGGATGCT 
GGGATATTTT TGGTCCTGGC CACGACGGGC CACTTCTCGC TTTTCCCTCT GCTCTTCACA 
GCGCCAGAAC TGCCCATTAA GGTGCTGCTG ATGTTGATCT TCACTGTATT TAGTGTCTCT 
GCCCTGAGAG CCATGTTTCA GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P