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manf cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Summary

Gene Symbol manf
Entrez Gene ID 549179
Full Name mesencephalic astrocyte-derived neurotrophic factor
Synonyms armet,arp
General protein information
Preferred Names

mesencephalic astrocyte-derived neurotrophic factor

Names

mesencephalic astrocyte-derived neurotrophic factor
arginine-rich, mutated in early stage tumors
Gene Type protein-coding
Organism Xenopus tropicalis(tropical clawed frog)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001016425.2 NP_001016425.1 mesencephalic astrocyte-derived neurotrophic factor precursor

Pathways from BioSystems


Homologies


Caenorhabditis elegans (roundworm) Y54G2A.23 NP_500273.2
Bos taurus (cattle) MANF NP_001094681.1
Drosophila melanogaster (fruit fly) Manf NP_477445.1
Homo sapiens (human) MANF NP_006001.3
Mus musculus (house mouse) Manf NP_083379.2
LOC700659 XP_002802839.1
Danio rerio (zebrafish) manf NP_001070097.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP003016 XP_311862.3
Pan troglodytes (chimpanzee) MANF XP_001169644.3
Canis lupus familiaris (dog) MANF XP_003639808.2
Xenopus tropicalis (tropical clawed frog) manf NP_001016425.1

Gene Ontology


Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following manf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the manf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa22537 NM_001016425.2
Latest version!		 Xenopus tropicalis mesencephalic astrocyte-derived neurotrophic factor (manf), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $104.30
$149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OXa22537
Clone ID Related Accession (Same CDS sequence) NM_001016425.2
Accession Version NM_001016425.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 543bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-21
Organism Xenopus tropicalis(tropical clawed frog)
Product mesencephalic astrocyte-derived neurotrophic factor precursor
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR761066.2. On Oct 14, 2005 this sequence version replaced NM_001016425.1. ##Evidence-Data-START## Transcript exon combination :: CR761066.2, CF224555.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00862004, SAMN00862008 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
ATGGTTCCTC TCGCCCTTAT TACTGTGACT GGGATGCTGG CTCTTCTGCC AAGCGATGCT 
GCAGCTCTGA AAGCCGGAGA TTGCGAGGTG TGCATTTCTT TCATGACCCG GTTGTACCAG 
TCCCTTAAAG AACGGAAAGT AGAGTTTAAG CCAGACGTTG TTGAAAAGGA ACTGCTAAAG 
ACCTGCAATG ATGCCAGAGG AAAAGAGAAC AGGCTGTGTT ACTACATTGG AGCCACCAGC 
GACGCTGCCA CCAAAATCAC AAACGAGGTG TCCAGACCAT TAAGTAACCA CATCCCTCCT 
GAGAAGATCT GCGAGAAGCT AAAGAAGAAA GATGGGCAGA TCTGTGAGCT GAAGTATGAT 
AAACAGATTG ACCTGAGCAC AGTGGATCTA AAGAAGCTCA AAGTCAAGGA ACTGAAGAAG 
ATTTTGGATG ACTGGGGCGA GTCCTGCAAG GGCTGTGCTG AGAAATCGGA CTTTATCCGC 
AAGATTAATG AACTGATGCC TAAATACGCT CCCAATGCAG CCAATGCACG GACTGACCTT 
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001016425.1
CDS23..565
Translation

Target ORF information:

RefSeq Version NM_001016425.2
Organism Xenopus tropicalis(tropical clawed frog)
Definition Xenopus tropicalis mesencephalic astrocyte-derived neurotrophic factor (manf), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001016425.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGTTCCTC TCGCCCTTAT TACTGTGACT GGGATGCTGG CTCTTCTGCC AAGCGATGCT 
GCAGCTCTGA AAGCCGGAGA TTGCGAGGTG TGCATTTCTT TCATGACCCG GTTGTACCAG 
TCCCTTAAAG AACGGAAAGT AGAGTTTAAG CCAGACGTTG TTGAAAAGGA ACTGCTAAAG 
ACCTGCAATG ATGCCAGAGG AAAAGAGAAC AGGCTGTGTT ACTACATTGG AGCCACCAGC 
GACGCTGCCA CCAAAATCAC AAACGAGGTG TCCAGACCAT TAAGTAACCA CATCCCTCCT 
GAGAAGATCT GCGAGAAGCT AAAGAAGAAA GATGGGCAGA TCTGTGAGCT GAAGTATGAT 
AAACAGATTG ACCTGAGCAC AGTGGATCTA AAGAAGCTCA AAGTCAAGGA ACTGAAGAAG 
ATTTTGGATG ACTGGGGCGA GTCCTGCAAG GGCTGTGCTG AGAAATCGGA CTTTATCCGC 
AAGATTAATG AACTGATGCC TAAATACGCT CCCAATGCAG CCAATGCACG GACTGACCTT 
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P