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mlc1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Summary

Gene Symbol mlc1
Entrez Gene ID 780383
Full Name megalencephalic leukoencephalopathy with subcortical cysts 1
Synonyms lvm,mlc,mlc1-3
General protein information
Preferred Names

megalencephalic leukoencephalopathy with subcortical cysts 1

Names

membrane protein MLC1
megalencephalic leukoencephalopathy with subcortical cysts 1 gene product
Gene Type protein-coding
Organism Xenopus tropicalis(tropical clawed frog)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_012959324.2 XP_012814778.1 membrane protein MLC1 isoform X2
NM_001079453.1 NP_001072921.1 membrane protein MLC1
XM_018092190.2 XP_017947679.1 membrane protein MLC1 isoform X1
XM_012959324.3 XP_012814778.1 membrane protein MLC1 isoform X2

Pathways from BioSystems


Homologies


MLC1 XP_001111622.1
Mus musculus (house mouse) Mlc1 NP_573504.1
Rattus norvegicus (Norway rat) Mlc1 NP_001101575.1
Danio rerio (zebrafish) mlc1 NP_001098409.1
Homo sapiens (human) MLC1 NP_631941.1
Gallus gallus (chicken) MLC1 XP_004937559.1
Xenopus tropicalis (tropical clawed frog) mlc1 NP_001072921.1
Canis lupus familiaris (dog) MLC1 XP_848790.2
Pan troglodytes (chimpanzee) MLC1 XP_515212.3
Bos taurus (cattle) MLC1 NP_001070420.1

Gene Ontology


Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mlc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mlc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa00938 NM_001079453.1
Latest version!		 Xenopus tropicalis megalencephalic leukoencephalopathy with subcortical cysts 1 (mlc1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OXa29769 XM_012959324.2
Latest version!		 Xenopus tropicalis megalencephalic leukoencephalopathy with subcortical cysts 1 (mlc1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OXa53710 XM_018092190.2
Latest version!		 Xenopus tropicalis megalencephalic leukoencephalopathy with subcortical cysts 1 (mlc1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OXa53711 XM_012959324.3
Latest version!		 Xenopus tropicalis megalencephalic leukoencephalopathy with subcortical cysts 1 (mlc1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OXa00938
Clone ID Related Accession (Same CDS sequence) NM_001079453.1
Accession Version NM_001079453.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1134bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-22
Organism Xenopus tropicalis(tropical clawed frog)
Product membrane protein MLC1
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC121460.1. ##Evidence-Data-START## Transcript exon combination :: BC121460.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028309, SAMD00028312 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGACCAGAG AAGAAGGGTT AATTGAAGAA TATGGTTATG ATCGAATGGC TACTTTAGAA 
CTGGGGAAGC AAGATAAGAA AAATCGCCAT ACAGATCTAA AGTCCAGTGA TATTCAGCTT 
CATGAGAGGC TTAAGCCATG TACTGGGTAC AAAATGTGGA TGTTTTCCTT GCTGATGGGG 
AGCTGCCTTA TTGCTACATC TGGATTTTCC CTTTATCTTG GCAATGTCTT TCCCTCTGAA 
ATGGACTACC TGCGTTGTGC TGCAGGCTCT TGTCTGCCTT CAGCGGTTGT CCATTTTGCT 
GTGCTGAAAA ACAAAGTTAA TGTGATGTAT CATTACCAAA TATTATTTGT GTCAACATTT 
TCAATAACTA CCACTTGTTT GATTTGGTTT GGATGCAAAC TCGCTATAAA CCCAACAGCA 
ATCAATATAA ACTTTAATCT GATTCTTCTT ATTCTGATGG AAATTCTTAT GGCAACAACA 
GTAATTATCT CTGCAAGATC AAATGTAGAC TGCTGTAAAG TACAGAGAGG CATGGCATAT 
GGGAGTTGTT TGAAGCAGAT TAAATTTCCT TCTCGACTGA TGAAATCTTA TTCAATTGTG 
GAGGTGATTG TAGGAGTTTG TGCAGTATTT GGTGGTGTTA TTGCACTTAA CTTTGATGCT 
CTGTTACACA GTCTTTACCT CTATGTATCC ATCTTCTGGG TATTAGCAGC GTGTTTTCCA 
AATGCCGTCG TAAGCCATAT AGCTGCTGAA TATCCCACTA AATGCTTGGT GGAGGGGCTC 
ATCGTGACAA GCAGTATAAC ATCTCCCTTG CTGTTTGCAA CGTCAGCCTT CCTGTCTTTC 
AGTATTATAA GGACCATTGA CATTTTTAGA ATTTACCCGG CAATTGTTAC ACATTTTCAT 
GATATTGTTT TATTTCTTCT GATGCTGATC CTGTTAGTGC AGGCCTTGCT CACAAATATC 
ACCATCATCC AATGTGTTCA CTACAAAACA TTGATGAGAA TAGATGATTT ATCCTGGAAC 
ATGTCACAGG TAGACAAGAT GGATTACAGA AATAATGATG TTTCCAACAA CACTTTAAGA 
GAATTTGACA AAGATAAAGC ATGGAAGGCA GTAGTGGTAC AAATGACACA ATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001072921.1
CDS94..1227
Translation

Target ORF information:

RefSeq Version NM_001079453.1
Organism Xenopus tropicalis(tropical clawed frog)
Definition Xenopus tropicalis megalencephalic leukoencephalopathy with subcortical cysts 1 (mlc1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001079453.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGACCAGAG AAGAAGGGTT AATTGAAGAA TATGGTTATG ATCGAATGGC TACTTTAGAA 
CTGGGGAAGC AAGATAAGAA AAATCGCCAT ACAGATCTAA AGTCCAGTGA TATTCAGCTT 
CATGAGAGGC TTAAGCCATG TACTGGGTAC AAAATGTGGA TGTTTTCCTT GCTGATGGGG 
AGCTGCCTTA TTGCTACATC TGGATTTTCC CTTTATCTTG GCAATGTCTT TCCCTCTGAA 
ATGGACTACC TGCGTTGTGC TGCAGGCTCT TGTCTGCCTT CAGCGGTTGT CCATTTTGCT 
GTGCTGAAAA ACAAAGTTAA TGTGATGTAT CATTACCAAA TATTATTTGT GTCAACATTT 
TCAATAACTA CCACTTGTTT GATTTGGTTT GGATGCAAAC TCGCTATAAA CCCAACAGCA 
ATCAATATAA ACTTTAATCT GATTCTTCTT ATTCTGATGG AAATTCTTAT GGCAACAACA 
GTAATTATCT CTGCAAGATC AAATGTAGAC TGCTGTAAAG TACAGAGAGG CATGGCATAT 
GGGAGTTGTT TGAAGCAGAT TAAATTTCCT TCTCGACTGA TGAAATCTTA TTCAATTGTG 
GAGGTGATTG TAGGAGTTTG TGCAGTATTT GGTGGTGTTA TTGCACTTAA CTTTGATGCT 
CTGTTACACA GTCTTTACCT CTATGTATCC ATCTTCTGGG TATTAGCAGC GTGTTTTCCA 
AATGCCGTCG TAAGCCATAT AGCTGCTGAA TATCCCACTA AATGCTTGGT GGAGGGGCTC 
ATCGTGACAA GCAGTATAAC ATCTCCCTTG CTGTTTGCAA CGTCAGCCTT CCTGTCTTTC 
AGTATTATAA GGACCATTGA CATTTTTAGA ATTTACCCGG CAATTGTTAC ACATTTTCAT 
GATATTGTTT TATTTCTTCT GATGCTGATC CTGTTAGTGC AGGCCTTGCT CACAAATATC 
ACCATCATCC AATGTGTTCA CTACAAAACA TTGATGAGAA TAGATGATTT ATCCTGGAAC 
ATGTCACAGG TAGACAAGAT GGATTACAGA AATAATGATG TTTCCAACAA CACTTTAAGA 
GAATTTGACA AAGATAAAGC ATGGAAGGCA GTAGTGGTAC AAATGACACA ATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OXa29769
Clone ID Related Accession (Same CDS sequence) XM_012959324.2
Accession Version XM_012959324.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1068bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-09-07
Organism Xenopus tropicalis(tropical clawed frog)
Product membrane protein MLC1 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959324.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGACCAGAG AAGAAGGGTT AATTGAAGAA TATGGTTATG ATCGAATGGC TACTTTAGAA 
CTGGGGAAGC AAGATAAGAA AAATCGCCAT ACAGATCTAA AGTCCAGTGA TATTCAGCTT 
CATGAGAGGC TTAAGCCATG TACTGGGTAC AAAATGTGGA TGTTTTCCTT GCTGATGGGG 
AGCTGCCTTA TTGCTACATC TGGATTTTCC CTTTATCTTG GCAATGTCTT TCCCTCTGAA 
ATGGACTACC TGCGTTGTGC TGCAGGCTCT TGTCTGCCTT CAGCGGTTGT CCATTTTGCT 
GTGCTGAAAA ACAAAGTTAA TGTGATGTAT CATTACCAAA TATTATTTGT GTCAACATTT 
TCAATAACTA CCACTTGTTT GATTTGGTTT GGATGCAAAC TCGCTATAAA CCCAACAGCA 
ATCAATATAA ACTTTAATCT GATTCTTCTT ATTCTGATGG AAATTCTTAT GGCAACAACA 
GTAATTATCT CTGCAAGATC AAATGTAGAC TGCTGTAAAG TACAGAGAAT TGTGGAGGTG 
ATTGTAGGAG TTTGTGCAGT ATTTGGTGGT GTTATTGCAC TTAACTTTGA TGCTCTGTTA 
CACAGTCTTT ACCTCTATGT ATCCATCTTC TGGGTATTAG CAGCGTGTTT TCCAAATGCC 
GTCGTAAGCC ATATAGCTGC TGAATATCCC ACTAAATGCT TGGTGGAGGG GCTCATCGTG 
ACAAGCAGTA TAACATCTCC CTTGCTGTTT GCAACGTCAG CCTTCCTGTC TTTCAGTATT 
ATAAGGACCA TTGACATTTT TAGAATTTAC CCGGCAATTG TTACACATTT TCATGATATT 
GTTTTATTTC TTCTGATGCT GATCCTGTTA GTGCAGGCCT TGCTCACAAA TATCACCATC 
ATCCAATGTG TTCACTACAA AACATTGATG AGAATAGATG ATTTATCCTG GAACATGTCA 
CAGGTAGACA AGATGGATTA CAGAAATAAT GATGTTTCCA ACAACACTTT AAGAGAATTT 
GACAAAGATA AAGCATGGAA GGCAGTAGTG GTACAAATGA CACAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_012814778.1
CDS1175..2242
Translation

Target ORF information:

RefSeq Version XM_012959324.2
Organism Xenopus tropicalis(tropical clawed frog)
Definition Xenopus tropicalis megalencephalic leukoencephalopathy with subcortical cysts 1 (mlc1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_012959324.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGACCAGAG AAGAAGGGTT AATTGAAGAA TATGGTTATG ATCGAATGGC TACTTTAGAA 
CTGGGGAAGC AAGATAAGAA AAATCGCCAT ACAGATCTAA AGTCCAGTGA TATTCAGCTT 
CATGAGAGGC TTAAGCCATG TACTGGGTAC AAAATGTGGA TGTTTTCCTT GCTGATGGGG 
AGCTGCCTTA TTGCTACATC TGGATTTTCC CTTTATCTTG GCAATGTCTT TCCCTCTGAA 
ATGGACTACC TGCGTTGTGC TGCAGGCTCT TGTCTGCCTT CAGCGGTTGT CCATTTTGCT 
GTGCTGAAAA ACAAAGTTAA TGTGATGTAT CATTACCAAA TATTATTTGT GTCAACATTT 
TCAATAACTA CCACTTGTTT GATTTGGTTT GGATGCAAAC TCGCTATAAA CCCAACAGCA 
ATCAATATAA ACTTTAATCT GATTCTTCTT ATTCTGATGG AAATTCTTAT GGCAACAACA 
GTAATTATCT CTGCAAGATC AAATGTAGAC TGCTGTAAAG TACAGAGAAT TGTGGAGGTG 
ATTGTAGGAG TTTGTGCAGT ATTTGGTGGT GTTATTGCAC TTAACTTTGA TGCTCTGTTA 
CACAGTCTTT ACCTCTATGT ATCCATCTTC TGGGTATTAG CAGCGTGTTT TCCAAATGCC 
GTCGTAAGCC ATATAGCTGC TGAATATCCC ACTAAATGCT TGGTGGAGGG GCTCATCGTG 
ACAAGCAGTA TAACATCTCC CTTGCTGTTT GCAACGTCAG CCTTCCTGTC TTTCAGTATT 
ATAAGGACCA TTGACATTTT TAGAATTTAC CCGGCAATTG TTACACATTT TCATGATATT 
GTTTTATTTC TTCTGATGCT GATCCTGTTA GTGCAGGCCT TGCTCACAAA TATCACCATC 
ATCCAATGTG TTCACTACAA AACATTGATG AGAATAGATG ATTTATCCTG GAACATGTCA 
CAGGTAGACA AGATGGATTA CAGAAATAAT GATGTTTCCA ACAACACTTT AAGAGAATTT 
GACAAAGATA AAGCATGGAA GGCAGTAGTG GTACAAATGA CACAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OXa53710
Clone ID Related Accession (Same CDS sequence) XM_018092190.2
Accession Version XM_018092190.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1134bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-17
Organism Xenopus tropicalis(tropical clawed frog)
Product membrane protein MLC1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018092190.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGACCAGAG AAGAAGGGTT AATTGAAGAA TATGGTTATG ATCGAATGGC TACTTTAGAA 
CTGGGGAAGC AAGATAAGAA AAATCGCCAT ACAGATCTAA AGTCCAGTGA TATTCAGCTT 
CATGAGAGGC TTAAGCCATG TACTGGGTAC AAAATGTGGA TGTTTTCCTT GCTGATGGGG 
AGCTGCCTTA TTGCTACATC TGGATTTTCC CTTTATCTTG GCAATGTCTT TCCCTCTGAA 
ATGGACTACC TGCGTTGTGC TGCAGGCTCT TGTCTGCCTT CAGCGGTTGT CCATTTTGCT 
GTGCTGAAAA ACAAAGTTAA TGTGATGTAT CATTACCAAA TATTATTTGT GTCAACATTT 
TCAATAACTA CCACTTGTTT GATTTGGTTT GGATGCAAAC TCGCTATAAA CCCAACAGCA 
ATCAATATAA ACTTTAATCT GATTCTTCTT ATTCTGATGG AAATTCTTAT GGCAACAACA 
GTAATTATCT CTGCAAGATC AAATGTAGAC TGCTGTAAAG TACAGAGAGG CATGGCATAT 
GGGAGTTGTT TGAAGCAGAT TAAATTTCCT TCTCGACTGA TGAAATCTTA TTCAATTGTG 
GAGGTGATTG TAGGAGTTTG TGCAGTATTT GGTGGTGTTA TTGCACTTAA CTTTGATGCT 
CTGTTACACA GTCTTTACCT CTATGTATCC ATCTTCTGGG TATTAGCAGC GTGTTTTCCA 
AATGCCGTTG TAAGCCATAT AGCTGCTGAA TATCCCACTA AATGCTTGGT GGAGGGGCTC 
ATCGTGACAA GCAGTATAAC ATCTCCCTTG CTGTTTGCAA CGTCAGCCTT CCTGTCTTTC 
AGTATTATAA GGACCATTGA CATTTTTAGA ATTTACCCGG CAATTGTTAC ACATTTTCAT 
GATATTGTTT TATTTCTTCT GATGCTGATC CTGTTAGTGC AGGCCTTGCT CACAAATATC 
ACCATCATCC AATGTGTTCA CTACAAAACA TTGATGAGAA TAGATGATTT ATCCTGGAAC 
ATGTCACAGG TAGACAAGAT GGATTACAGA AATAATGATG TTTCCAACAA CACTTTAAGA 
GAATTTGACA AAGATAAAGC ATGGAAGGCA GTAGTGGTAC AAATGACACA ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_017947679.1
CDS84..1217
Translation

Target ORF information:

RefSeq Version XM_018092190.2
Organism Xenopus tropicalis(tropical clawed frog)
Definition Xenopus tropicalis megalencephalic leukoencephalopathy with subcortical cysts 1 (mlc1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_018092190.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGACCAGAG AAGAAGGGTT AATTGAAGAA TATGGTTATG ATCGAATGGC TACTTTAGAA 
CTGGGGAAGC AAGATAAGAA AAATCGCCAT ACAGATCTAA AGTCCAGTGA TATTCAGCTT 
CATGAGAGGC TTAAGCCATG TACTGGGTAC AAAATGTGGA TGTTTTCCTT GCTGATGGGG 
AGCTGCCTTA TTGCTACATC TGGATTTTCC CTTTATCTTG GCAATGTCTT TCCCTCTGAA 
ATGGACTACC TGCGTTGTGC TGCAGGCTCT TGTCTGCCTT CAGCGGTTGT CCATTTTGCT 
GTGCTGAAAA ACAAAGTTAA TGTGATGTAT CATTACCAAA TATTATTTGT GTCAACATTT 
TCAATAACTA CCACTTGTTT GATTTGGTTT GGATGCAAAC TCGCTATAAA CCCAACAGCA 
ATCAATATAA ACTTTAATCT GATTCTTCTT ATTCTGATGG AAATTCTTAT GGCAACAACA 
GTAATTATCT CTGCAAGATC AAATGTAGAC TGCTGTAAAG TACAGAGAGG CATGGCATAT 
GGGAGTTGTT TGAAGCAGAT TAAATTTCCT TCTCGACTGA TGAAATCTTA TTCAATTGTG 
GAGGTGATTG TAGGAGTTTG TGCAGTATTT GGTGGTGTTA TTGCACTTAA CTTTGATGCT 
CTGTTACACA GTCTTTACCT CTATGTATCC ATCTTCTGGG TATTAGCAGC GTGTTTTCCA 
AATGCCGTTG TAAGCCATAT AGCTGCTGAA TATCCCACTA AATGCTTGGT GGAGGGGCTC 
ATCGTGACAA GCAGTATAAC ATCTCCCTTG CTGTTTGCAA CGTCAGCCTT CCTGTCTTTC 
AGTATTATAA GGACCATTGA CATTTTTAGA ATTTACCCGG CAATTGTTAC ACATTTTCAT 
GATATTGTTT TATTTCTTCT GATGCTGATC CTGTTAGTGC AGGCCTTGCT CACAAATATC 
ACCATCATCC AATGTGTTCA CTACAAAACA TTGATGAGAA TAGATGATTT ATCCTGGAAC 
ATGTCACAGG TAGACAAGAT GGATTACAGA AATAATGATG TTTCCAACAA CACTTTAAGA 
GAATTTGACA AAGATAAAGC ATGGAAGGCA GTAGTGGTAC AAATGACACA ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OXa53711
Clone ID Related Accession (Same CDS sequence) XM_012959324.3
Accession Version XM_012959324.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1068bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-17
Organism Xenopus tropicalis(tropical clawed frog)
Product membrane protein MLC1 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959324.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGACCAGAG AAGAAGGGTT AATTGAAGAA TATGGTTATG ATCGAATGGC TACTTTAGAA 
CTGGGGAAGC AAGATAAGAA AAATCGCCAT ACAGATCTAA AGTCCAGTGA TATTCAGCTT 
CATGAGAGGC TTAAGCCATG TACTGGGTAC AAAATGTGGA TGTTTTCCTT GCTGATGGGG 
AGCTGCCTTA TTGCTACATC TGGATTTTCC CTTTATCTTG GCAATGTCTT TCCCTCTGAA 
ATGGACTACC TGCGTTGTGC TGCAGGCTCT TGTCTGCCTT CAGCGGTTGT CCATTTTGCT 
GTGCTGAAAA ACAAAGTTAA TGTGATGTAT CATTACCAAA TATTATTTGT GTCAACATTT 
TCAATAACTA CCACTTGTTT GATTTGGTTT GGATGCAAAC TCGCTATAAA CCCAACAGCA 
ATCAATATAA ACTTTAATCT GATTCTTCTT ATTCTGATGG AAATTCTTAT GGCAACAACA 
GTAATTATCT CTGCAAGATC AAATGTAGAC TGCTGTAAAG TACAGAGAAT TGTGGAGGTG 
ATTGTAGGAG TTTGTGCAGT ATTTGGTGGT GTTATTGCAC TTAACTTTGA TGCTCTGTTA 
CACAGTCTTT ACCTCTATGT ATCCATCTTC TGGGTATTAG CAGCGTGTTT TCCAAATGCC 
GTTGTAAGCC ATATAGCTGC TGAATATCCC ACTAAATGCT TGGTGGAGGG GCTCATCGTG 
ACAAGCAGTA TAACATCTCC CTTGCTGTTT GCAACGTCAG CCTTCCTGTC TTTCAGTATT 
ATAAGGACCA TTGACATTTT TAGAATTTAC CCGGCAATTG TTACACATTT TCATGATATT 
GTTTTATTTC TTCTGATGCT GATCCTGTTA GTGCAGGCCT TGCTCACAAA TATCACCATC 
ATCCAATGTG TTCACTACAA AACATTGATG AGAATAGATG ATTTATCCTG GAACATGTCA 
CAGGTAGACA AGATGGATTA CAGAAATAAT GATGTTTCCA ACAACACTTT AAGAGAATTT 
GACAAAGATA AAGCATGGAA GGCAGTAGTG GTACAAATGA CACAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_012814778.1
CDS1285..2352
Translation

Target ORF information:

RefSeq Version XM_012959324.3
Organism Xenopus tropicalis(tropical clawed frog)
Definition Xenopus tropicalis megalencephalic leukoencephalopathy with subcortical cysts 1 (mlc1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_012959324.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGACCAGAG AAGAAGGGTT AATTGAAGAA TATGGTTATG ATCGAATGGC TACTTTAGAA 
CTGGGGAAGC AAGATAAGAA AAATCGCCAT ACAGATCTAA AGTCCAGTGA TATTCAGCTT 
CATGAGAGGC TTAAGCCATG TACTGGGTAC AAAATGTGGA TGTTTTCCTT GCTGATGGGG 
AGCTGCCTTA TTGCTACATC TGGATTTTCC CTTTATCTTG GCAATGTCTT TCCCTCTGAA 
ATGGACTACC TGCGTTGTGC TGCAGGCTCT TGTCTGCCTT CAGCGGTTGT CCATTTTGCT 
GTGCTGAAAA ACAAAGTTAA TGTGATGTAT CATTACCAAA TATTATTTGT GTCAACATTT 
TCAATAACTA CCACTTGTTT GATTTGGTTT GGATGCAAAC TCGCTATAAA CCCAACAGCA 
ATCAATATAA ACTTTAATCT GATTCTTCTT ATTCTGATGG AAATTCTTAT GGCAACAACA 
GTAATTATCT CTGCAAGATC AAATGTAGAC TGCTGTAAAG TACAGAGAAT TGTGGAGGTG 
ATTGTAGGAG TTTGTGCAGT ATTTGGTGGT GTTATTGCAC TTAACTTTGA TGCTCTGTTA 
CACAGTCTTT ACCTCTATGT ATCCATCTTC TGGGTATTAG CAGCGTGTTT TCCAAATGCC 
GTTGTAAGCC ATATAGCTGC TGAATATCCC ACTAAATGCT TGGTGGAGGG GCTCATCGTG 
ACAAGCAGTA TAACATCTCC CTTGCTGTTT GCAACGTCAG CCTTCCTGTC TTTCAGTATT 
ATAAGGACCA TTGACATTTT TAGAATTTAC CCGGCAATTG TTACACATTT TCATGATATT 
GTTTTATTTC TTCTGATGCT GATCCTGTTA GTGCAGGCCT TGCTCACAAA TATCACCATC 
ATCCAATGTG TTCACTACAA AACATTGATG AGAATAGATG ATTTATCCTG GAACATGTCA 
CAGGTAGACA AGATGGATTA CAGAAATAAT GATGTTTCCA ACAACACTTT AAGAGAATTT 
GACAAAGATA AAGCATGGAA GGCAGTAGTG GTACAAATGA CACAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P