The High-Throughput DNA Library Assembly Service offers accurate and efficient assembly and cloning of various predefined DNA fragments or parts into a vector of customer choice to generate a library of cloned constructs strategically assembled in a specific arrangement. These predefined DNA fragments or parts may be of promoters, enhancers/repressors, specific binding sites, localization signals, genes, terminators, and barcodes. Such libraries are powerful tools often utilized by scientists for a variety of combinatorial optimization applications, such as identification of an optimal genetic circuit design for the best possible output and optimization of a metabolic pathway to maximize the production a high-value chemical, biofuel, or pharmaceutical.
Built on our advanced, high-throughput platforms and world-renowned expertise in gene synthesis, GenScript offers comprehensive High-Throughput DNA Library Assembly services for various downstream screening needs to help accelerate your metabolic pathway and microbial strain engineering process.
We offer arrayed libraries providing up to 10,000 individual, precisely assembled constructs. Each assembled construct contains up to 4 variable slots for inserts of up to 15 kb total length, cloned into your vector of choice, with up to 4 µg in yield.
|Library Description||Up to 10,000 individual, precisely assembled constructs based on the exact design|
|Screening||Every single design in the library|
|Deliverables||1.5 ml tubes or 96-well plates|
|Quality Control||Sequence verification of all delivered constructs|
|Arrayed Library Assembly|
Over the last several years, GenScript has partnered with researchers in both industry and academia to build sophisticated combinatorial DNA libraries. These highly-customized libraries were used to develop new drugs, enzymes, or to discover novel gene functions and networks. The following is one example of such successful partnerships:
You can use our custom-built combinatorial DNA libraries in a variety of applications across many disciplines of life sciences, from plant biology to drug development. The following lists three major areas of combinatorial DNA library applications:
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