The High-Throughput DNA Library Assembly Service offers accurate and efficient assembly and cloning of various predefined DNA fragments or parts into a vector of customer choice to generate a library of cloned constructs strategically assembled in a specific arrangement. These predefined DNA fragments or parts may be of promoters, enhancers/repressors, specific binding sites, localization signals, genes, terminators, and barcodes. Such libraries are powerful tools often utilized by scientists for a variety of combinatorial optimization applications, such as identification of an optimal genetic circuit design for the best possible output and optimization of a metabolic pathway to maximize the production a high-value chemical, biofuel, or pharmaceutical.

Built on our advanced, high-throughput platforms and world-renowned expertise in gene synthesis, GenScript offers comprehensive High-Throughput DNA Library Assembly services for various downstream screening needs to help accelerate your metabolic pathway and microbial strain engineering process.



high-throughput solution

Seamlessly assembled

with advanced methods

Expert advising

on all project plans by our Ph.D.-level scientists

Faster and more economical

compared to your in-house operations

Arrayed format

with up to 1x108 constructs containing 4 variable slots for 15 kb inserts

Free storage

for all your synthesized projects with CloneArk™ Storage System

Substarte Small

Service Features

We offer arrayed libraries providing up to 10,000 individual, precisely assembled constructs. Each assembled construct contains up to 4 variable slots for inserts of up to 15 kb total length, cloned into your vector of choice, with up to 4 µg in yield.

Arrayed Library
Library Description Up to 10,000 individual, precisely assembled constructs based on the exact design
Screening Every single design in the library
Deliverables 1.5 ml tubes or 96-well plates
Quality Control Sequence verification of all delivered constructs


Example Workflow

Arrayed Library Assembly

  • Case Studies

  • Applications

Over the last several years, GenScript has partnered with researchers in both industry and academia to build sophisticated combinatorial DNA libraries. These highly-customized libraries were used to develop new drugs, enzymes, or to discover novel gene functions and networks. The following is one example of such successful partnerships:


Arrayed High-Throughput DNA Library Assembly

Optimizing Gene Circuits for Therapeutic Applications

Learn More

An Efficient and Accurate DNA Assembly Service for Rapid Metabolic Pathway Optimization

Download Application Note

How to Order

Our customer service representatives are available 24 hours Monday through Friday. You may contact us anytime for assistance. Orders can be placed online, over the phone, by email or fax. Please have your payment information (credit card, purchase order or bank information) ready.

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Mutagenesis Services

Get in Touch with GenScript Custom Mutagenesis Services
Mutagenesis Expression | Recombinant Mutagenesis & rAb Services - GenScript protein2022
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