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High-Throughput DNA Library Assembly Service

The High-Throughput DNA Library Assembly Service offers accurate and efficient assembly and cloning of various predefined DNA fragments or parts into a vector of customer choice to generate a library of cloned constructs strategically assembled in a specific arrangement. These predefined DNA fragments or parts may be of promoters, enhancers/repressors, specific binding sites, localization signals, genes, terminators, and barcodes. Such libraries are powerful tools often utilized by scientists for a variety of combinatorial optimization applications, such as identification of an optimal genetic circuit design for the best possible output and optimization of a metabolic pathway to maximize the production a high-value chemical, biofuel, or pharmaceutical.

Built on our advanced, high-throughput platforms and world-renowned expertise in gene synthesis, GenScript offers comprehensive High-Throughput DNA Library Assembly services for various downstream screening needs to help accelerate your metabolic pathway and microbial strain engineering process.

Advantages

Service Features

We offer arrayed libraries providing up to 10,000 individual, precisely assembled constructs. Each assembled construct contains up to 4 variable slots for inserts of up to 15 kb total length, cloned into your vector of choice, with up to 4 µg in yield.

Workflow
Arrayed Library Assembly
Case Studies Applications Over the last several years, GenScript has partnered with researchers in both industry and academia to build sophisticated combinatorial DNA libraries. These highly-customized libraries were used to develop new drugs, enzymes, or to discover novel gene functions and networks. The following is one example of such successful partnerships: Case Study: Arrayed High-Throughput DNA Library Assembly -- Optimizing Gene Circuits for Therapeutic Applications You can use our custom-built combinatorial DNA libraries in a variety of applications across many disciplines of life sciences, from plant biology to drug development. The following lists three major areas of combinatorial DNA library applications: Synthetic Biology Metabolic Pathway and Microbial Strain Engineering: screen and optimize your synthetic molecular pathways to develop the most efficient metabolic gene circuits for chemical, drug, and biofuel production. Genome Engineering: screen high-throughput libraries of synthetic nuclease- or CRISPR/Cas9-based constructs for highly effective genome editing. Protein Engineering Create new multi-domain proteins for structural or functional screening. Optimize protein function through directed evolution. Generate recombinant protein or chimeric antibody libraries for high-throughput screening for biologic drug discovery and development. Gene Expression Regulation Determine the optimal combination of promoter, ribosome binding site, terminator, and other regulatory elements for your construct or expression system. Create a library of reporter-gene constructs for systematic promoter analysis. Express any gene of interest under the control of different promoters, e.g. for inducible or tissue-specific manipulation of lethal or developmentally vital genes.
How to Order
Arrayed Library Assembly Quote/Order Request Form Download and complete the appropriate request form below and email it back to [email protected]. Our Ph.D. scientists will be in touch within 24 hours to discuss your project.

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