His-tag is one of the most widely applied tags for recombinant protein expression and purification. It is also used for binding assay to detect protein-protein interactions by a pull-down assay or in conjunction with anti-His antibody as IP/Co-IP assay. The three new high quality mAb based assay products will provide convenient, easy, and robust tools for different purposes, including purification and detection of His-tagged fusion-protein for downstream assays, enrichment and screening of His-tagged fusion-protein mutants, and Co-IP & screening of interacting proteins.
His Tag ELISA Detection Kit
GenScript His Tag Protein Detection Kit is developed for the detection of His-tagged protein
expression and
its relative quantification using His-tagged protein standards of known molecular weight (M.W.). This kit is
based on competitive ELISA, suitable for most systems, such as bacterial, yeast, mammalian cell lysates and
cell culture supernatant. This kit also offers a His-tagged protein stock for customer recovery verification
assay.
Semi-quantitation with known His-tagged protein as standards
Example
Conc. of His Tag Standard
OD450
(ng/ml)
(pmol/ml)
Duplicate 1
Duplicate 2
Average
0
0
2.177
2.183
2.18
1
0.088
1.833
1.831
1.832
3
0.265
1.374
1.418
1.396
9
0.796
0.825
0.922
0.874
27
2.389
0.516
0.444
0.48
81
7.168
0.266
0.289
0.278
243
21.504
0.158
0.157
0.158
729
64.513
0.105
0.101
0.103
Typical assay results and standard curve using His Tag ELISA Detection Kit
His Tag Antibody Plate
His Tag Antibody Plate is a microtiter plate coated with THE™ Anti His Tag Antibody. The
plate is
designed for capturing His tagged protein with His tag located at N-terminus, C-terminus, or internal of the
protein. It can be applied for analysis of different length of His tag including Hexa-His (6 His), Penta-His
(5-His) and Tetra-His (4-His).The plate demonstrate high sensitivity and high binding capacity. The capacity
and sensitivity varies, depending on protein size, structure and solution buffer. Generally, proteins with
lower molecular weight (M.W) can be more sensitive to the His antibody plate than ones with higher M.W.
Compared with Nickel Coated Plate, the affinity plate can bind target proteins with high specificity, and
tolerable with several common used reagents such as EDTA, imidazole and β-ME.
DYKDDDDK (Flag) Tag Antibody Plate is a microtiter plate coated with THE™ Anti DYKDDDDK
(Flag) Tag
Antibody. The plate is designed for the rapid capturing of DYKDDDDK (Flag) tagged protein expressed in
different systems, including, E. coli, yeast and mammalian extracts and cell culture supernatants. It can be
applied for the detection of DYKDDDDK (Flag) tag located at N-terminus, C-terminus, or internal of the
protein. The plate demonstrates high sensitivity and high binding capacity. The capacity and sensitivity
varies, depending on protein size, structure and solution buffer. Generally, proteins with lower molecular
weight (M.W) can be more sensitive to the DYKDDDDK (Flag) antibody plate than ones with higher M.W. The plate
is tolerable with several common used reagents. Avoid using detection reagents that can recognize mouse IgG,
such as anti mouse IgG antibodies and protein A.
