Overview

His-tag is one of the most widely applied tags for recombinant protein expression and purification. It is also used for binding assay to detect protein-protein interactions by a pull-down assay or in conjunction with anti-His antibody as IP/Co-IP assay. The three new high quality mAb based assay products will provide convenient, easy, and robust tools for different purposes, including purification and detection of His-tagged fusion-protein for downstream assays, enrichment and screening of His-tagged fusion-protein mutants, and Co-IP & screening of interacting proteins.

His Tag ELISA Detection Kit

GenScript His Tag Protein Detection Kit is developed for the detection of His-tagged protein expression and its relative quantification using His-tagged protein standards of known molecular weight (M.W.). This kit is based on competitive ELISA, suitable for most systems, such as bacterial, yeast, mammalian cell lysates and cell culture supernatant. This kit also offers a His-tagged protein stock for customer recovery verification assay.

Cat. No. Product Name Quantity Price Order
L00436
His Tag ELISA Detection Kit
$298.00 1 kit
 
 

Applications

Detect His-tagged proteins

Screen optimal expression conditions

Semi-quantitation with known His-tagged protein as standards

Example

Conc. of His Tag Standard OD450
(ng/ml) (pmol/ml) Duplicate 1 Duplicate 2 Average
0 0 2.177 2.183 2.18
1 0.088 1.833 1.831 1.832
3 0.265 1.374 1.418 1.396
9 0.796 0.825 0.922 0.874
27 2.389 0.516 0.444 0.48
81 7.168 0.266 0.289 0.278
243 21.504 0.158 0.157 0.158
729 64.513 0.105 0.101 0.103

Typical assay results and standard curve using His Tag ELISA Detection Kit

His Tag Antibody Plate

His Tag Antibody Plate is a microtiter plate coated with THE™ Anti His Tag Antibody. The plate is designed for capturing His tagged protein with His tag located at N-terminus, C-terminus, or internal of the protein. It can be applied for analysis of different length of His tag including Hexa-His (6 His), Penta-His (5-His) and Tetra-His (4-His).The plate demonstrate high sensitivity and high binding capacity. The capacity and sensitivity varies, depending on protein size, structure and solution buffer. Generally, proteins with lower molecular weight (M.W) can be more sensitive to the His antibody plate than ones with higher M.W. Compared with Nickel Coated Plate, the affinity plate can bind target proteins with high specificity, and tolerable with several common used reagents such as EDTA, imidazole and β-ME.

Cat. No. Product Name Quantity Price Order
L00440b
His Tag Antibody Plate (black, 96 wells, nonremovable)
$179.00 5 plates
 
L00440c
His Tag Antibody Plate (clear, 8x12 strips)
$179.00 5 plates
 
L00440w
His Tag Antibody Plate (white, 96 wells, nonremovable)
$179.00 5 plates
 

Other Tagged Protein Detection & Purification

DYKDDDDK(Flag) Tag Antibody Plate (96-well)

DYKDDDDK (Flag) Tag Antibody Plate is a microtiter plate coated with THE™ Anti DYKDDDDK (Flag) Tag Antibody. The plate is designed for the rapid capturing of DYKDDDDK (Flag) tagged protein expressed in different systems, including, E. coli, yeast and mammalian extracts and cell culture supernatants. It can be applied for the detection of DYKDDDDK (Flag) tag located at N-terminus, C-terminus, or internal of the protein. The plate demonstrates high sensitivity and high binding capacity. The capacity and sensitivity varies, depending on protein size, structure and solution buffer. Generally, proteins with lower molecular weight (M.W) can be more sensitive to the DYKDDDDK (Flag) antibody plate than ones with higher M.W. The plate is tolerable with several common used reagents. Avoid using detection reagents that can recognize mouse IgG, such as anti mouse IgG antibodies and protein A.

Cat. No. Product Name Quantity Price Order
L00455B
DYKDDDDK Tag Antibody Plate (Black, 96-well)
$229.00 5 plates
 
L00455C
DYKDDDDK Tag Antibody Plate (Clear, 8X12 strip)
$229.00 5 plates
 
L00455W
DYKDDDDK Tag Antibody Plate (White, 96-well)
$229.00 5 plates
 

Related products

Cat. No. Product Name Quantity Price Order
A00174
His-tag Antibody, pAb, Rabbit
$102.00 40 μg
 
A00186
THE™ His Tag Antibody, mAb, Mouse
$385.00 100 μg
 
A00205
His-tag antibody [Biotin], pAb, Rabbit
$102.00 40 μg
 
A00612
THE™ His Tag Antibody [HRP], mAb, Mouse
$425.00 100 μg
 
A00613
THE™ His Tag Antibody [Biotin], mAb, Mouse
$425.00 100 μg
 
A01620
THE™ His Tag Antibody [FITC], mAb, Mouse
$425.00 100 μg
 
L00223
High Affinity Ni-Charged Resin
$83.00
 
L00295
Ni-charged MagBeads
$101.00 8 ml (2 ml Settled Beads)
 

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