|QC Items||Analytical Method||Specification|
|Appearance||Visual inspection||Clear, no visible particles|
|A260/280||Nanodrop UV absorption||1.8-2.0|
|Concentration||UV absorption||Default 1 mg/ml†; Specific concentration provided upon request|
|Sequence Accuracy New!||Sequence verification of full gene insert||100% alignment with reference sequence*|
|Homogeneity||Agarose gel electrophoresis||90%±10% Supercoiled**|
|Restriction Analysis||Enzyme digestion and electrophoresis||Conforming to reference pattern|
|Residual RNA||Visual inspection upon electrophoresis||Not visible|
|Genomic DNA||Visual inspection upon electrophoresis||Not visible***|
|Endotoxin LevelWhat's||Limulus Amebocyte Lysate (LAL) assay||≤0.01 EU/μg|
|Quantitative LAL assay, Optional New!|
|Bioburden Assay||Bioburden||No growth on agar plate after 48 hours|
Note: † The reading of plasmid
concentration may varies in the results of multiple measurements, with the approximate range from -5~+15%.
* Sequence verification of full gene insert only applies when bundled with gene synthesis (an insert to be cloned into a plasmid).
** Percentage of supercoiled plasmid may vary when analyzing with different methods or can be altered due to shipping conditions.
*** Since densitometry is semi quantitative, the genomic DNA is considered nonvisible if the comparative intensity of genomic DNA < 15% of plasmid band. Quantitative genomic DNA measurement by qPCR is available with additional charges.
For more information about our QC measures, click here.
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