QC Items | Analytical Method | Specification |
---|---|---|
Appearance | Visual inspection | Clear, no visible particles |
A260/280 | Nanodrop UV absorption | 1.8-2.0 |
Concentration | UV absorption | Default 1 mg/ml†; Specific concentration provided upon request |
Sequence Accuracy New! | Sequence verification of full gene insert | 100% alignment with reference sequence* |
Homogeneity | Agarose gel electrophoresis | 90%±10% Supercoiled** |
Restriction Analysis | Enzyme digestion and electrophoresis | Conforming to reference pattern |
Residual RNA | Visual inspection upon electrophoresis | Not visible |
Genomic DNA | Visual inspection upon electrophoresis | Not visible*** |
Endotoxin LevelWhat's | Limulus Amebocyte Lysate (LAL) assay | ≤0.01 EU/μg |
Quantitative LAL assay, Optional New! | ||
Bioburden Assay | Bioburden | No growth on agar plate after 48 hours |
Note: † The reading of plasmid
concentration may varies in the results of multiple measurements, with the approximate range from -5~+15%.
* Sequence verification of full gene insert only applies when bundled with gene synthesis (an insert to be
cloned into a plasmid).
** Percentage of supercoiled plasmid may vary when analyzing with different methods or can be altered due to
shipping conditions.
*** Since densitometry is semi quantitative, the genomic DNA is considered nonvisible if the comparative
intensity of genomic DNA < 15% of plasmid band. Quantitative genomic DNA measurement by qPCR is available with
additional charges.
For more information about our QC
measures, click here.
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