Targeted Lipid Nanoparticle Formulation
For Enhanced RNA Delivery

Targeted LNP Service

Quality Control and Specification

Case Studies

• T Cell Targeted LNP: CD3-Ab/LNP
  

  Fluc mRNA(N1-methyl-pseU) was encapsulated with SM102-LNP or SM102-LNP decorated with anti-Human CD3 Antibody  (Clone: OKT3, mAb, Mouse, GenScript, A02199), 100ng of mRNA encapsulated in LNP were incubated with jurkat cells in RPMI140 +10%FBS at 37C for 24 hrs. The expression of Fluc was detected using Luciferase Assay (Promega, E4030).

  

  eGFP mRNA(N1-methyl-pseU) was encapsulated with SM102-LNP or SM102-LNP decorated with anti-Human CD3 Antibody  (Clone OKT3, mAb, Mouse, GenScript), 10-150ng of mRNA encapsulated in LNP were incubated with primary T cells  for 24 hrs. The expression of eGFP was detected using flow cytometry. W1, W2 and W3 indicate different wash conditions.

• CD34+ Cell Targeted LNP: CD34-Ab/LNP
  

  EGFP mRNA(N1-methyl-pseU) was encapsulated with SM102-LNP or SM102-LNP decorated with anti-Human CD34 Antibody (Purified anti-human CD34 antibody(Anti-Human CD34 Antibody, mAb, Mouse, GenScript, CP0001) 100ng of mRNA encapsulated in LNP were incubated with Kasumi-1 cells  for 24 hrs. The expression of eGFP was detected using flow cytometry.

  

  EGFP mRNA(N1-methyl-pseU) was encapsulated with ALC0315-LNP or ALC0315-LNP decorated with anti-Human CD34 Antibody (Purified anti-human CD34 antibody(Anti-Human CD34 Antibody, mAb, Mouse, GenScript, CP0001) 100ng of mRNA encapsulated in LNP were incubated with primary human CD34+ cells  for 24 hrs. The expression of eGFP was detected using flow cytometry.

• Liver Targeted LNP: Tri-GalNac/LNP
  

  

  Fluc mRNA(N1-methyl-pseU) was encapsulated with LNP , 0.3mg/kg of mRNA encapsulated in LNP were injection through tail vein into mice, after 24 hrs, mice were sacrificed, and the expression of Fluc mRNA was measured with whole body imaging with different organs.

  

  EGFP mRNA(N1-methyl-pseU) was encapsulated with SM102-LNP or SM102-LNP decorated with TriGalNac, 0.3mg/kg of mRNA encapsulated in LNP were injection through tail vein into mice, after 24 hrs, mice were sacrificed, and liver organs were lysed. The eGFP expression in lysate was detected using western blot method.

• Liver Targeted LNP: 20DODAP LNP
  

  

  Fluc mRNA(N1-methyl-pseU) was encapsulated with LNP , 0.3mg/kg of mRNA encapsulated in LNP were injection through tail vein into mice, after 24 hrs, mice were sacrificed, and the expression of Fluc mRNA was measured with whole body imaging with different organs.

• Spleen Targeted LNP: SM10218PA LNP
  

  

  Fluc mRNA(N1-methyl-pseU) was encapsulated with LNP , 0.3mg/kg of mRNA encapsulated in LNP were injection through tail vein into mice, after 24 hrs, mice were sacrificed, and the expression of Fluc mRNA was measured with whole body imaging with different organs.

• Lung Targeted LNP: SORT 50DOTAP LNP
  

  

  Fluc mRNA(N1-methyl-pseU) was encapsulated with LNP , 0.3mg/kg of mRNA encapsulated in LNP were injection through tail vein into mice, after 24 hrs, mice were sacrificed, and the expression of Fluc mRNA was measured with whole body imaging with different organs.

• Lung Targeted LNP: SM102COMP3 LNP
  

  EGFP mRNA(N1-methyl-pseU) was encapsulated with SM102-LNP or SM102-LNP decorated with anti-Human CD117 Antibody (Purified anti-human CD117 (c-kit) Antibody, Clone 104D2, Biolegend), 100ng of mRNA encapsulated in LNP were incubated with Kasumi-1 cells  for 24 hrs. The expression of eGFP was detected using flow cytometry.

  

  EGFP mRNA(N1-methyl-pseU) was encapsulated with SM102-LNP or SM102-LNP decorated with anti-Human CD117 Antibody (Purified anti-human CD117 (c-kit) Antibody, Clone 104D2, Biolegend), 10ug of mRNA encapsulated in LNP were injection into mice IV. The expression of eGFP in menatopoietics cells of the BM, Ckit+Lin, LSK and SLAM cells were detected using flow cytometry.