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Catalog Products » Protein Isolation and Analysis » Protein Isolation and Purification » Protein Purification Resins » Glutathione Resin

Glutathione Resin


Description GenScript Glutathione Resin (L00206) is an affinity chromatography medium designed for easy, one-step purification of recombinant glutathione S-transferase (GST) fusion proteins and other glutathione binding proteins expressed in E. coli, insect cells and mammalian cells. The recombinant GST fusion proteins can be purified directly from pre-treated cell lysate using Glutathione Resin. It is the excellent choice for high performance purifications.
The GST Fusion Protein Purification Kit(L00207) also includes five disposable columns and five 0.154 g samples of glutathione to facilitate protein purification.
GenScript Protein Expression and Purification Kit (L00208) provide all the major reagents and materials for the cloning, expression and purification of target protein. The target protein is first expressed as a GST fusion protein in E. coli using the GenScript expression vector pGS-21a. The GST fusion protein is bound to a column packed with GenScript Glutathione Resin (L00206) and then eluted with reduced glutathione. Finally, to obtain the tag-free target protein, the purified GST fusion protein is digested with recombinant porcine Enterokinase (EK) to remove GST tag.
Storage:Store at 4°C,do NOT freeze.

Product Details
Cat. No.Product Name Volume supplied Resin content
L00206Glutathione Resin (10 ml)20 ml50% slurry
Stability Stable for 18 months if stored unopened
Key Features

Key Features:
  • Easy to use: Simple and fast procedures for purification of GST-fusion proteins.
  • High capacity: The system can support over 34-45 mg GST-fusion proteins (26 kDa) /ml medium.
  • Stability: This reusable resin shows no obvious decrease of the binding capacity after three uses.

Storage Store at 4°C, do NOT freeze.

Cat. No.
Product Name
Size
Price
Quantity
A00130-40 GST-tag Antibody [HRP], pAb, Rabbit
40 ug
$99.00
L00207 GST Fusion Protein Purification Kit
1 kit
$139.00
 
Petrosyan A., et al. Non-muscle myosin IIA transports a Golgi glycosyltransferase to the endoplasmic reticulum by binding to its cytoplasmic tail. Int J Biochem Cell Biol. 2012 Jul;44(7):1153-65.


Ali MF., et al.Golgi phosphoprotein 3 determines cell binding properties under dynamic flow by controlling Golgi localization of core 2 N-acetylglucosaminyltransferase 1.J Biol Chem. 2012 Nov 16;287(47):39564-77.


Bobba S., et al. Microtiter plate-based assay for inhibitors of penicillin-binding protein 2a from methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother. 2011 Jun;55(6):2783-7.


Ma L, et al. Identification of SUMO Substrates with Diverse Functions Using the Xenopus Egg Extract System. Mol Cell Proteomics. 2014May;

Y Zhang, et al. Identification of Mycoplasma suis MSG1 interaction proteins on porcine erythrocytes. Arch Microbiol. 2014Qct.;

D Li, et al. Over-expression of ArathEULS3 confers ABA sensitivity and drought tolerance in Arabidopsis. Plant Cell Tissue Organ Cult. 2014Feb.;

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Cat. No. L00206
Size
Price
10 ml $98.00
 
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