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Catalog Products » Protein Isolation and Analysis » Protein Isolation and Purification » Protein Purification Resins » Protein Expression and Purification Kit

Protein Expression and Purification Kit


Description GenScript Glutathione Resin (L00206) is an affinity chromatography medium designed for easy, one-step purification of recombinant glutathione S-transferase (GST) fusion proteins and other glutathione binding proteins expressed in E. coli, insect cells and mammalian cells. The recombinant GST fusion proteins can be purified directly from pre-treated cell lysate using Glutathione Resin. It is the excellent choice for high performance purifications. Table 1 lists the main characteristics of Glutathione Resin.
The GST Fusion Protein Purification Kit(L00207) also includes five disposable columns and five 0.154 g samples of glutathione to facilitate protein purification.
GenScript Protein Expression and Purification Kit (L00208) provide all the major reagents and materials for the cloning, expression and purification of target protein. The target protein is first expressed as a GST fusion protein in E. coli using the GenScript expression vector pGS-21a. The GST fusion protein is bound to a column packed with GenScript Glutathione Resin (L00206) and then eluted with reduced glutathione. Finally, to obtain the tag-free target protein, the purified GST fusion protein is digested with recombinant porcine Enterokinase (EK) to remove GST tag.
Storage:Store at 4°C,do NOT freeze.
Key Features

Key Features:
  • Easy to perform: Simple and fast procedure for purification of GST-fusion proteins.
  • High capacity: The system can support over 6 mg GST-fusion proteins (26 kDa) /ml medium.
  • Stability: This reusable resin shows no obvious decrease of the binding capacity after three uses.
Storage See manual for detail storage information

Cat. No.
Product Name
Size
Price
Quantity
A00130-40 GST-tag Antibody [HRP], pAb, Rabbit
40 ug
$99.00
A00202-100 GST-tag Antibody [Biotin], pAb, Rabbit
100 ug
$199.00
A00865-100 THETM GST Antibody, mAb, Mouse
100 ug
$175.00
A00865-40 THETM GST Antibody, mAb, Mouse
40 ug
$69.00
A00865-500 THETM GST Antibody, mAb, Mouse
500 ug
$750.00
A00867-200 THETM GST Antibody [Biotin], mAb, Mouse
200 ug
$333.00
Z02039-1 Glutathione S-Transferase
1 mg
$65.00
 


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Cat. No. L00208
Size
Price
1 kit $335.00
24 hour
 
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