Catalog Products » Protein Isolation and Analysis » Protein Isolation and Purification » Protein Purification Resins » Alkaline-tolerant Resin » Protein G Resin

Protein G Resin

Description GenScript Protein G Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and cell culture media. Protein G Resin can also be used for immunoprecipitation of proteins,protein complexes or antigens. The recombinant protein G ligand is coupled to 4% highly cross-linked agarose. The static binding capacity of Protein G Resin is over 20 mg goat IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.
Protein G, a bacterial cell wall protein isolated from group G Streptococci, binds to mammalian IgGs mainly through Fc regions. Native protein G has 3 IgG binding domains and also sites for albumin and cell-surface binding. The latter have been eliminated from recombinant protein G to reduce nonspecific binding. Although protein G has very similar tertiary structures to protein A, their amino acid compositions differ significantly, resulting in different binding characteristics. Protein G can be used for purification of mammalian monoclonal and polyclonal IgGs that do not bind well to protein A. Protein G has greater affinity than protein A for most mammalian IgGs, especially for certain subclasses including human IgG3, mouse IgG1 and rat IgG2a. Unlike protein A, protein G does not bind to human IgM, IgD and IgA.
Note: High temperature heating is not recommended. The agarose melts above 65°C.

Cat. No.Product Name Volume supplied Resin content
L00209Protein G Resin (5ml)10 ml50% slurry

Storage:Store at 4°C, do NOTfreeze.
Key Features
Key Features
  • Broad IgG binding spectrum
  • Binding specificity complements of protein A
  • Agarose media
  • No specific albumin binding
  • Optimized homogeneous recombinant ligand
  • High capacity
  • Available in bulk size

LigandRecombinant Streptococcal Protein G lacking the albumin-binding produced in E. coli
Number of IgG binding sites per ligand3
MW of ligandApproximately 22 kDa
PI of ligand4.69
Degree of substitution Approximately 2 mg protein G/ml settled resin
Static binding capacity >20 mg goat IgG/ml settled resin
Matrix spherical agarose, 4% cross-linked
Average particle size 90 μm (45-165 μm)
Storage solution1X PBS containing 20% ethanol
Storage & Stability 18 months if store unopened at 2-8℃

Storage Store at 2-8°C, do NOT freeze.

Cat. No.
Product Name
Protein G - 5 mg
Tubon TC Jr., et al. dCREB2-Mediated Enhancement of Memory Formation. J Neurosci. 2013 Apr 24;33(17):7475-87.

Muth S., et al. Release Of Dendritic Cells From Cognate Cd4 T-Cell Recognition Results In Impaired Peripheral Tolerance And Fatal Cytotoxic T-Cell Mediated Autoimmunity. Proc Natl Acad Sci. 2012 Jun 5;109(23):9059-64.

Bikkavilli RK., et al. Wnt3a-stimulated LRP6 phosphorylation is dependent upon arginine methylation of G3BP2. J Cell Sci. 2012 May 15;125(Pt 10):2446-56.

Matthew Staron, et al. Heat-shock protein gp96/grp94 is an essential chaperone for the platelet glycoprotein Ib-IX-V complex. Blood. 2011 Jun 30;117(26):7136-44.

Wiriya Chiranand, et al. The CTA4 Transcription Factor Mediates Induction of Nitrosative Stress Response in Candida albicans. Eukaryot Cell. 2008 Feb;7(2):268-78.

Armistead JS., et al.Antibodies to a single, conserved epitope in Anopheles APN1 inhibit universal transmission of falciparum and vivax malaria. Infect Immun.2013 Dec 9.

Muth S, et al. A CD40/CD40L feedback loop drives the breakdown of CD8 T‐cell tolerance following depletion of suppressive CD4 T cells. Eur J Immunol. 2014Jan.;

Short KR, et al. Antibodies mediate formation of neutrophil extracellular traps in the middle ear and facilitate secondary pneumococcal otitis media. Infect Immun. 2014Jan.;82(1):364-70

CW Armitage, et al. Divergent outcomes following transcytosis of IgG targeting intracellular and extracellular chlamydial antigens. Immunol Cell Biol. 2014Jan.;

Kan W, et al. M3 Muscarinic Receptor Interaction with Phospholipase C β3 Determines its Signaling Efficiency. J Biol Chem. 2014Mar.;

N Zilio, et al. A Novel HDAC Complex in the Control of Transcription and Genome Stability. Mol Cell Biol. 2014July;

Zhang Y, et al. Heterologous expression of AtPAP2 in transgenic potato influences carbon metabolism and tuber development. FEBS Lett. 2014Aug.;

P Zhao, et al. Generation and Characterization of Polyclonal Antibody Against Part of Immunoglobulin Constant Heavy υ Chain of Goose. Monoclon Antib Immunodiagn Immunother. 2014Aug.;33(4):287-90

L Feng, et al. Negative feedback regulation of Wnt signaling via N-linked fucosylation in zebrafish. Dev Biol. 2014Sep.;

Hwang JH, et al. Polyoma Small T Antigen Interacts with YES-Associated Protein (YAP) to Regulate Cell Survival and Differentiation. J Virol. 2014Aug.;

Hwang JH, et al. Polyoma Small T Antigen Interacts with YES-Associated Protein (YAP) to Regulate Cell Survival and Differentiation. J Virol. 2014Aug.;

You can visit "Citations Database" for more citations.

For more documents, please visit "Technical Support".

Cat. No. L00209
5 ml $129.00