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Catalog Products » Protein Isolation and Analysis » Protein Isolation and Purification » Protein Purification Resins » Protein G Resin

Protein G Resin


Description GenScript Protein G Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and cell culture media. Protein G Resin can also be used for immunoprecipitation of proteins,protein complexes or antigens. The recombinant protein G ligand is coupled to 4% highly cross-linked agarose. The static binding capacity of Protein G Resin is over 20 mg goat IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.
Protein G, a bacterial cell wall protein isolated from group G Streptococci, binds to mammalian IgGs mainly through Fc regions. Native protein G has 3 IgG binding domains and also sites for albumin and cell-surface binding. The latter have been eliminated from recombinant protein G to reduce nonspecific binding. Although protein G has very similar tertiary structures to protein A, their amino acid compositions differ significantly, resulting in different binding characteristics. Protein G can be used for purification of mammalian monoclonal and polyclonal IgGs that do not bind well to protein A. Protein G has greater affinity than protein A for most mammalian IgGs, especially for certain subclasses including human IgG3, mouse IgG1 and rat IgG2a. Unlike protein A, protein G does not bind to human IgM, IgD and IgA.
Note: High temperature heating is not recommended. The agarose melts above 65°C.

Cat. No.Product Name Volume supplied Resin content
L00209Protein G Resin (5ml)10 ml50% slurry

Storage:Store at 4°C, do NOTfreeze.
Key Features
Key Features
  • Broad IgG binding spectrum
  • Binding specificity complements of protein A
  • Agarose media
  • No specific albumin binding
  • Optimized homogeneous recombinant ligand
  • High capacity
  • Available in bulk size

Specifications
LigandRecombinant Streptococcal Protein G lacking the albumin-binding produced in E. coli
Number of IgG binding sites per ligand3
MW of ligandApproximately 22 kDa
PI of ligand4.69
Degree of substitution Approximately 2 mg protein G/ml settled resin
Static binding capacity >20 mg goat IgG/ml settled resin
Matrix spherical agarose, 4% cross-linked
Average particle size 90 μm (45-165 μm)
Storage solution1X PBS containing 20% ethanol
Storage & Stability 18 months if store unopened at 2-8℃

Storage Store at 4°C
Protein G Resin
Protein G Resin

SDS-PAGE of binding capacity detection of Protein G Resin (L00209)
Lane 1: Flow (X corporation)
Lane 2: Wash (X corporation)
Lane 3: Elute (X corporation)
Lane 4: Flow (GenScript)
Lane 5: Wash (GenScript)
Lane 6: Elute (GenScript)
Lane 7: Smart Protein Marker (GenScript, MM0900)


Cat. No.
Product Name
Size
Price
Quantity
Z02007 Protein G
5 mg
$95.00
 
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Cat. No. L00209
Size
Price
5 ml $129.00
 
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