• English
  • Sign In
  • Contact Us
Catalog Products » Protein Analysis & Purification » Protein Isolation and Purification » Protein Purification Resins » Protein G Resin

Protein G Resin

GenScript Protein G Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and cell culture media. The recombinant protein G ligand is coupled to 4% highly cross-linked agarose. The static binding capacity of Protein G Resin is over 20 mg sheep IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.

Protein G, a bacterial cell wall protein isolated from group G Streptococci, binds to mammalian IgGs mainly through Fc regions. Native protein G has 3 IgG binding domains and also sites for albumin and cell-surface binding. The latter have been eliminated from recombinant protein G to reduce nonspecific binding. Although protein G has very similar tertiary structures to protein A, their amino acid compositions differ significantly, resulting in different binding characteristics. Protein G can be used for purification of mammalian monoclonal and polyclonal IgGs that do not bind well to protein A. Protein G has greater affinity than protein A for most mammalian IgGs, especially for certain subclasses including human IgG3, mouse IgG1 and rat IgG2a. Unlike protein A, protein G does not bind to human IgM, IgD and IgA.

Total Volume:
5 ml settled resin (10 ml 50% slurry)

Storage Store at 2-8°C, do NOT freeze.

Cat. No. Product Name Quantity Price
Protein G - 5 mg
Candas-Green D, et al. Dual blockade of CD47 and HER2 eliminates radioresistant breast cancer cells. Nature Communications. (2020-09)

Xu K, et al. Lack of AKAP3 disrupts integrity of the subcellular structure and proteome of mouse sperm and causes male sterility. Development (Cambridge, England). (2020-01)

Hara H, et al. The NLRP6 Inflammasome Recognizes Lipoteichoic Acid and Regulates Gram-Positive Pathogen Infection. Cell. (2018-11)

Zhuang W, et al. Generation and characterization of human B lymphocyte stimulator blocking monoclonal antibody. Mol Immunol. (2016-09)

Sathiyamoorthy K, et al. Structural basis for Epstein-Barr virus host cell tropism mediated by gp42 and gHgL entry glycoproteins. Nat Commun. (2016)

Ringel P, et al. Enzymatic characterization of recombinant nitrate reductase expressed and purified from Neurospora crassa. Fungal Genet Biol. (2015-04)

Hwang JH, et al. Polyoma Small T Antigen Interacts with YES-Associated Protein (YAP) to Regulate Cell Survival and Differentiation. J Virol. (2014-08)

Hwang JH, et al. Protein Phosphatase 2A Isoforms Utilizing Aβ Scaffolds Regulate Differentiation through Control of Akt. J Biol Chem. (2013-09)

Bikkavilli RK, et al. Wnt3a-stimulated LRP6 phosphorylation is dependent upon arginine methylation of G3BP2. J Cell Sci. (2012-05)

Lei Y, et al. The mitochondrial proteins NLRX1 and TUFM form a complex that regulates type I interferon and autophagy. Immunity. (2010-06)

Jay H. Lo and Thomas T. Chen., et al. CCAAT/Enhancer Binding Protein β2 Is Involved in Growth Hormone-Regulated Insulin-Like Growth Factor-II Gene Expression in the Liver of Rainbow Trout (Oncorhynchus mykiss). Endocrinology. (2010-05)

Rapti K, et al. Neutralizing antibodies against AAV serotypes 1, 2, 6, and 9 in sera of commonly used animal models. Mol Ther. (2010-01)

You can visit "Citations Database" for more citations.

For more documents, please visit "Technical Support".

Cat. No. L00209
Size Price
5 ml Resin (Total 10 ml) $129.00