Catalog Products » Protein Isolation and Purification » Protein Purification Resins » Protein A Resin

Protein A Resin

GenScript Protein A Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Protein A Resin can also be used for immunoprecipitation of proteins, protein complexes or antigens. The recombinant protein A ligand is coupled to 4% agarose. The coupling is optimized to give high binding capacity for immunoglobulins. The static binding capacity of Protein A Resin is greater than 20 mg human IgG/ml settled resin. The binding capacity will vary depending on several factors such as target antibody, flow rate etc. Table 1 lists the characteristics of Protein A Resin.
L00210
$108.00

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Description

GenScript Protein A Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Protein A Resin can also be used for immunoprecipitation of proteins, protein complexes or antigens. The recombinant protein A ligand is coupled to 4% agarose. The coupling is optimized to give high binding capacity for immunoglobulins. The static binding capacity of Protein A Resin is greater than 20 mg human IgG/ml settled resin. The binding capacity will vary depending on several factors such as target antibody, flow rate etc. Table 1 lists the characteristics of Protein A Resin.


Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs mainly through Fc regions. Native protein A has five IgG binding domains and many other domains with unknown functions. Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Since only the Fc region is involved in binding, the Fab region is available for binding antigens.

Note

High temperature heating is not recommended. The agarose melts above 65°C.

Overview
Product Information Characteristics of Protein A Resin
Resin Volume5ml settled resin (10 ml 50% slurry)
LigandRecombinant Streptococcal protein A expressed in E. coli
Number of IgG binding sites per ligand5
M.W. of ligandApproximately 34 kDa
PI of ligand5.17
Degree of substitutionApproximately 2 mg protein A/ml settled resin
Static binding capacity
> 20 mg human IgG/ml settled resin
Matrix sphericalAgarose, 4% 
Average particle size90 μm (45-165 μm)
Storage solution20% ethanol 
Storage conditions2-8 °C;  Do not freeze

Properties
Storage & Stability Store regenerated Protein A Resin in 20% ethanol at 2°C to 8°C. Do not freeze.


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