Protein A Resin
L00210 | |
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$107.80 | |
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Ask us a question |
L00210 | |
|
|
$107.80 | |
|
|
|
|
Ask us a question |
GenScript Protein A Resin is an affinity
chromatography medium designed for easy, one-step purification of classes, subclasses
and fragments of immunoglobulins from biological fluids and from cell culture
media. Protein A Resin can also be used for immunoprecipitation of
proteins, protein complexes or antigens. The recombinant protein A ligand is
coupled to 4%
agarose. The coupling is optimized to give high binding capacity for
immunoglobulins. The static binding
capacity of Protein A Resin is greater than 20 mg human IgG/ml settled resin.
The binding capacity will
vary depending on several factors such as target antibody, flow rate etc. Table
1 lists the characteristics of Protein A Resin. Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs mainly through Fc regions. Native protein A has five IgG binding domains and many other domains with unknown functions. Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Since only the Fc region is involved in binding, the Fab region is available for binding antigens. |
High temperature heating is not recommended. The agarose melts above 65°C.
Product Information |
Characteristics of Protein A Resin
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Storage & Stability |
Store regenerated Protein A Resin in 20% ethanol at 2°C to 8°C. Do not freeze. |