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Catalog Products » Protein Analysis & Purification » Protein Isolation and Purification » Protein Purification Resins » Protein A Resin

Protein A Resin

Description GenScript Protein A Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Protein A Resin can also be used for immunoprecipitation of proteins,protein complexes or antigens. The recombinant protein A ligand is coupled to 4% agarose. The coupling is optimized to give high binding capacity for immunoglobulins. The static binding capacity of Protein A Resin is greater than 20 mg human IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.
Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus , binds to mammalian IgGs mainly through Fc regions. Native protein A has five IgG binding domains and many other domains with unknown functions. Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Since only the Fc region is involved in binding, the Fab region is available for binding antigens.
Note: High temperature heating is not recommended. The agarose melts above 65°C.

Cat. No.Product Name Volume supplied Resin content
L00210Protein A Resin (5ml)10 ml50% slurry
Store at 2-8°C, do NOT freeze.
Key Features
Key Features
  • High IgG-binding capacity
  • Resin is reusable up to 10 times with no significant loss of binding capacity.

Ligand Recombinant Streptococcal protein A expressed in E. coli
Number of IgG binding sites per ligand 5
MW of ligand Approximately 34 kDa
PI of ligand 5.17
Degree of substitution Approximately 2 mg protein A/ml settled resin
Static binding capacity >20 mg human IgG/ml settled resin
Matrix spherical agarose, 4%
Average particle size 90 μm (45-165 μm)
Storage solution1X PBS containing 20% ethanol
Storage & Stability 18 months when stored unopened at 2-8℃

Storage Store at 2-8°C, do NOT freeze.

Cat. No. Product Name Quantity Price
Protein A Antibody [HRP], pAb, Chicken - 40 ug
Hwang JH, et al. Polyoma Small T Antigen Interacts with YES-Associated Protein (YAP) to Regulate Cell Survival and Differentiation. J Virol. 2014Aug.;

Zhang H, et al. Longitudinally extensive NMO spinal cord pathology produced by passive transfer of NMO-IgG in mice lacking complement inhibitor CD59. J Autoimmun. 2014Mar.;

EC Martin,et al. Community-acquired meticillin-resistant Staphylococcus aureus strain USA300 resists staphylococcal protein A modulation by antibiotics and antimicrobial peptides. Int J Antimicrob Agents. 2014Qct.;

J Ratelade, et al. Human immunoglobulin g reduces the pathogenicity of aquaporin-4 autoantibodies in neuromyelitis optica. Exp Neurol. 2014Mar.;

D Chatterjee, et al. Mechanistic insight into the conserved allosteric regulation of periplasmic proteolysis by the signaling molecule cyclic-di-GMP. Elife. 2014Sep.;3e03650


J Ren, et al. G-protein αq participates in the steroid hormone 20-hydroxyecdysone nongenomic signal transduction. J Steroid Biochem Mol Biol. 2014Aug.;144PB313-323

Gow DJ, et al. Characterisation of a Novel Fc Conjugate of Macrophage Colony-stimulating Factor. Mol Ther. 2014Sep.;22(9):1580-92

J Ratelade, et al. Inhibitor (s) of the classical complement pathway in mouse serum limit the utility of mice as experimental models of neuromyelitis optica. Mol Immunol. 2014June;62(1):104-113

Payapilly A, et al. BAG6 regulates the quality control of a polytopic ERAD substrate. J Cell Sci. 2014July;127(Pt 13):2898-909

Asavapanumas N, et al. Neuromyelitis optica pathology in rats following intraperitoneal injection of NMO-IgG and intracerebral needle injury. Acta Neuropathol Commun. 2014April;248

Zhang H, et al. Longitudinally extensive NMO spinal cord pathology produced by passive transfer of NMO-IgG in mice lacking complement inhibitor CD59. J Autoimmun. 2014Mar.;

Lydia Wunderley, et al. The molecular basis for selective assembly of the UBAP1-containing endosome-specific ESCRT-I complex. J Cell Sci. 2014Feb.;127(Pt 3):663-672

W Liu, et al. In a non-genomic action, steroid hormone 20-hydroxyecdysone induces phosphorylation of cyclin-dependent kinase 10 to promote gene transcription. Endocrinology. 2014Feb.;

Xu K, et al. Sperm-specific AKAP3 is a dual specificity anchoring protien that interacts with both protein kinase A regulatory subunits via conserved N-terminal amphipathic peptides. Mol Reprod Dev. 2014Mar.;

XW Wang, et al.C-type lectin binds to β-integrin to promote hemocytic phagocytosis in an invertebrate. J Biol Chem. 2013 Dec 9.

Mabashi-Asazuma H., et al.A novel baculovirus vector for the production of non-fucosylated recombinant glycoproteins in insect cells. Glycobiology. 2013 Dec 20.

Liu W., et al.The hormone-dependent function of Hsp90 in the crosstalk between 20-hydroxyecdysone and juvenile hormone signaling pathways in insects is determined by differential phosphorylation and protein interactions. Biochim Biophys Acta. 2013 Nov;1830(11):5184-92.

Asavapanumas N., et al.Unique neuromyelitis optica pathology produced in naïve rats by intracerebral administration of NMO-IgG. Acta Neuropathol. 2013 Nov 5.

Lan JF., et al.Prohibitin interacts with envelope proteins of white spot syndrome virus and prevents infection in red swamp crayfish Procambarus clarkii. J Virol. 2013 Dec;87(23):12756-65.

Hwang JH., et al.Protein Phosphatase 2A Isoforms Utilizing Aβ Scaffolds Regulate Differentiation through Control of Akt. J Biol Chem. 2013 Nov 1; 288(44):32064-73.

Yin Y., et al. Differentially Expressed Genes of Human Microvascular Endothelial Cells in Response to Anti-Dengue Virus NS1 Antibodies by Suppression Subtractive Hybridization. Viral Immunol. 2013 Jun;26(3):185-91.

Dong DJ., et al. Steroid hormone 20-hydroxyecdysone regulation of the very-high-density lipoprotein (VHDL) receptor phosphorylation for VHDL uptake. Insect Biochem Mol Biol. 2013 Apr;43(4):328-35.

Song SE., et al. IGFBP5 mediates high glucose-induced cardiac fibroblast activation. J Mol Endocrinol. 2013 Apr 12;50(3):291-303.

Tradtrantip L., et al. \x09Therapeutic Cleavage of Anti–Aquaporin-4 Autoantibody in Neuromyelitis Optica by an IgG-Selective Proteinase. Mol Pharmacol. 2013 Jun;83(6):1268-75.

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Cat. No. L00210
Size Price
5 ml $98.00