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Catalog Products » Protein Isolation and Analysis » Protein Isolation and Purification » Protein Purification Resins » Protein A Resin

Protein A Resin


Description GenScript Protein A Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Protein A Resin can also be used for immunoprecipitation of proteins,protein complexes or antigens. The recombinant protein A ligand is coupled to 4% agarose. The coupling is optimized to give high binding capacity for immunoglobulins. The static binding capacity of Protein A Resin is greater than 20 mg human IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.
Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus , binds to mammalian IgGs mainly through Fc regions. Native protein A has five IgG binding domains and many other domains with unknown functions. Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Since only the Fc region is involved in binding, the Fab region is available for binding antigens.
Note: High temperature heating is not recommended. The agarose melts above 65°C.

Cat. No.Product Name Volume supplied Resin content
L00210Protein A Resin (5ml)10 ml50% slurry
Storage: Store at 4°C, do NOT freeze.
Key Features
Key Features
  • High IgG-binding capacity
  • Resin is reusable up to 10 times with no significant loss of binding capacity.

Characteristics:
Ligand Recombinant Streptococcal protein A expressed in E. coli
Number of IgG binding sites per ligand 5
MW of ligand Approximately 34 kDa
PI of ligand 5.17
Degree of substitution Approximately 2 mg protein A/ml settled resin
Static binding capacity >20 mg human IgG/ml settled resin
Matrix spherical agarose, 4%
Average particle size 90 μm (45-165 μm)
Storage solution1X PBS containing 20% ethanol
Storage & Stability 18 months when stored unopened at 2-8℃


Storage Store at 4°C, do NOT freeze.

Cat. No.
Product Name
Size
Price
Quantity
A00728-40 Protein A Antibody, pAb, Chicken
40 ug
$99.00
A00729-40 Protein A Antibody [HRP], pAb, Chicken
40 ug
$99.00
 
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Cat. No. L00210
Size
Price
5 ml $98.00
24 hour
 
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