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Catalog Products » Protein Isolation and Analysis » Protein Isolation and Purification » Protein Purification Resins » High Affinity Ni-Charged Resin

High Affinity Ni-Charged Resin


Description GenScript High Affinity Ni-Charged Resin is an 4% cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. High Affinity Ni-Charged Resin has low Ni2+ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni-Charged Resin the excellent choice for high performance purification of polyhistidine-tagged proteins. High Affinity Ni-Charged Resin is available in 10, 25 and 500 ml lab packs.
Note:High temperature heating is not recommended. The agarose melts above 65°C.

Cat. No.Product Name Volume supplied Resin content
L00223-10High Affinity Ni-Charged Resin(10 ml)20 ml50% slurry
L00223-25High Affinity Ni-Charged Resin(25 ml)50 ml50% slurry
Key Features
Key Features:
  • High binding capacity: The kit can handle over 45-60mg 6xHis-tagged protein/ml (CV).
  • Simple purification procedure under both native and denaturing conditions.
  • Broad pH stability: The resin works well between pH 3 and pH 13, and can manage pH 2 and pH 14 for short periods.
  • Resilient: If properly regenerated, the resin can be reused multiple times.

Storage Store at 4°C

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Cat. No. L00223
Size
Price
25 ml $168.00
10 ml $80.00
24 hour
 
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