High Affinity Ni-Charged Resin

GenScript High Affinity Ni-Charged Resin is an 4% cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. High Affinity Ni-Charged Resin has low Ni2+ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni-Charged Resin the excellent choice for high performance purification of polyhistidine-tagged proteins.

Price	$83.00
Cat. No.	L00223-10
Size	10 ml Resin (Total 20 ml) 25 ml Resin (Total 50 ml) 500 ml Resin (Total 1000 ml) Buy in bulk
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Overview Properties Applications

Description

GenScript High Affinity Ni-Charged Resin is an 4% cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. High Affinity Ni-Charged Resin has low Ni²⁺ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni-Charged Resin the excellent choice for high performance purification of polyhistidine-tagged proteins.

Note

High temperature heating is not recommended. The agarose melts above 65°C.

Overview

Product Information

Cat. No.	Product Name	Volume supplied	Resin content
L00223-10	High Affinity Ni-Charged Resin(10 ml)	20 ml	50% slurry
L00223-25	High Affinity Ni-Charged Resin(25 ml)	50 ml	50% slurry
L00223-500	High Affinity Ni-Charged Resin(500 ml)	1000 ml	50% slurry

Key Features

Characteristics of High Affinity Ni-Charged Resin

Matrix spherica	4% cross-linked agarose
Average particle size	90 μm (45-165 μm)
Dynamic binding capacity	≥20 mg of 6xHis-tagged protein (27 kDa) /ml settled resin
Storage solution	1X PBS containing 20% ethanol

Reagents Compatible with High Affinity Ni-Charged Resin

Denaturants	Detergents	Reducing agents	Salts	Others
6 M Gu·HCl	2% Triton X-100	20 mM β-ME	4 M MgCl₂	50% glycerol
8 M Urea	2% Tween 20	1 mM DTT	5 mM CaCl₂	20% ethanol
——	1% CHAPS	——	2 M NaCl	1 mM EDTA

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Properties

Storage & Stability

2-8 °C; DO NOT FREEZE

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Applications

Applications:

1. Purification of polyhistidine-tagged proteins under native conditions.
2. Purification of polyhistidine-tagged proteins from E. coli under denaturing conditions.

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Citations

1. Li Liu, et al. A Specific Sinorhizobium Flagellin Suppresses Legume Nodulation Through Immune Activation. Plant Biotechnology Journal. (2026-02)
2. Binita Shah; Moritz Hunkeler; Ariana Bratt; Hong Yue; Isabella Jaen Maisonet; Eric S. Fischer; Sara J. Buhrlage, et al. Structural basis of VCP-VCPIP1-p47 ternary complex in Golgi maintenance. Nature Communications. (2021-02)
3. Binita Shah; Moritz Hunkeler; Ariana Bratt; Hong Yue; Isabella Jaen Maisonet; Eric S. Fischer; Sara J. Buhrlage, et al. Structural basis of VCP-VCPIP1-p47 ternary complex in Golgi maintenance. Nature Communications. (2021-02)
4. Binita Shah; Moritz Hunkeler; Ariana Bratt; Hong Yue; Isabella Jaen Maisonet; Eric S. Fischer; Sara J. Buhrlage, et al. Structural basis of VCP-VCPIP1-p47 ternary complex in Golgi maintenance. Nature Communications. (2021-02)
5. Binita Shah; Moritz Hunkeler; Ariana Bratt; Hong Yue; Isabella Jaen Maisonet; Eric S. Fischer; Sara J. Buhrlage, et al. Structural basis of VCP-VCPIP1-p47 ternary complex in Golgi maintenance. Nature Communications. (2021-02)
6. View Runting Li, et al. Characterization of Isoforms of the Ovine Granulocyte Colony Stimulating Factor. biorxiv. (2019)
7. HsuKuan-Wei, et al. The synergy between RSC， Nap1 and adjacent nucleosome in nucleosome remodeling. Biochim Biophys Acta Gene Regul Mech. (2019)
8. Xu X, et al. Obtaining a mutant of Bacillus amyloliquefaciens xylanase A with improved catalytic activity by directed evolution. Enzyme Microb Technol. (2016-05)