Catalog Products » Protein Analysis & Purification » Protein Isolation and Purification » Protein Purification Resins » High Affinity Ni-Charged Resin

High Affinity Ni-Charged Resin

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Overview

Description

GenScript High Affinity Ni-Charged Resin is an 4% cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. High Affinity Ni-Charged Resin has low Ni²⁺ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni-Charged Resin the excellent choice for high performance purification of polyhistidine-tagged proteins.

Key Features

Characteristics of High Affinity Ni-Charged Resin

Matrix spherica	4% cross-linked agarose
Average particle size	90 μm (45-165 μm)
Dynamic binding capacity	≥20 mg of 6xHis-tagged protein (27 kDa) /ml settled resin
Storage solution	1X PBS containing 20% ethanol

Reagents Compatible with High Affinity Ni-Charged Resin

Denaturants	Detergents	Reducing agents	Salts	Others
6 M Gu·HCl	2% Triton X-100	20 mM β-ME	4 M MgCl₂	50% glycerol
8 M Urea	2% Tween 20	1 mM DTT	5 mM CaCl₂	20% ethanol
——	1% CHAPS	——	2 M NaCl	1 mM EDTA

Product Information

Cat. No.	Product Name	Volume supplied	Resin content
L00223-10	High Affinity Ni-Charged Resin(10 ml)	20 ml	50% slurry
L00223-25	High Affinity Ni-Charged Resin(25 ml)	50 ml	50% slurry
L00223-500	High Affinity Ni-Charged Resin(500 ml)	1000 ml	50% slurry

Properties

Storage	2-8 °C; DO NOT FREEZE
Note	High temperature heating is not recommended. The agarose melts above 65°C.

Applications

Applications:

1. Purification of polyhistidine-tagged proteins under native conditions.
2. Purification of polyhistidine-tagged proteins from E. coli under denaturing conditions.

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Citations

Zhang D., et al. The Acid Phosphatase-Encoding Gene GmACP1 Contributes to Soybean Tolerance to Low-Phosphorus Stress. PLoS Genet. 2014 Jan;10(1): e1004061.

Albert CR., et al. Effect of Hydroxychloroquine on Antiphospholipid Antibody-Induced Changes in First Trimester Trophoblast Function. Am J Reprod Immunol. 2014 Feb;71(2):154-64.

Chai Y., et al. A fibrinogen-related protein (TfFREP2) gene involving in the immune response of Trachidermus fasciatus against Vibrio anguillarum. Fish Shellfish Immunol. 2013 Dec;35(6):1988-92.

Worzfeld T., et al. ErbB-2 signals through Plexin-B1 to promote breast cancer metastasis. J Clin Invest. 2012 Apr 2;122(4):1296-305.

Sun J., et al. Apoptotic effect of Vibrio vulnificus cytolysin on A549 human lung adenocarcinoma cells. Mol Med Rep. 2012 Mar;5(3):668-74.

Zhao T., et al. Expression of Human Globular Adiponectin-Glucagon-Like Peptide-1 Analog Fusion Protein and Its Assay of Glucose-Lowering Effect In Vivo. Int J Med Sci. 2011 Mar 4.

Jiang HS., et al. A single whey acidic protein domain containing protein (SWD) inhibits bacteria invasion and dissemination in shrimp Marsupenaeus japonicus. Fish Shellfish Immunol. 2013 Aug;35(2):310-8.

de Oliveira S., et al. Cxcl8 (IL-8) mediates neutrophil recruitment and behavior in the zebrafish inflammatory response. J Immunol. 2013 Apr 15;190(8):4349-59.

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