High Affinity Ni-Charged Resin

GenScript High Affinity Ni-Charged Resin is an 4% cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. High Affinity Ni-Charged Resin has low Ni2+ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni-Charged Resin the excellent choice for high performance purification of polyhistidine-tagged proteins.

Price	$83.00
Cat. No.	L00223-10
Size	10 ml Resin (Total 20 ml) 25 ml Resin (Total 50 ml) 500 ml Resin (Total 1000 ml) Buy in bulk
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Overview Properties Applications

Description

GenScript High Affinity Ni-Charged Resin is an 4% cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. High Affinity Ni-Charged Resin has low Ni²⁺ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni-Charged Resin the excellent choice for high performance purification of polyhistidine-tagged proteins.

Note

High temperature heating is not recommended. The agarose melts above 65°C.

Overview

Product Information

Cat. No.	Product Name	Volume supplied	Resin content
L00223-10	High Affinity Ni-Charged Resin(10 ml)	20 ml	50% slurry
L00223-25	High Affinity Ni-Charged Resin(25 ml)	50 ml	50% slurry
L00223-500	High Affinity Ni-Charged Resin(500 ml)	1000 ml	50% slurry

Key Features

Characteristics of High Affinity Ni-Charged Resin

Matrix spherica	4% cross-linked agarose
Average particle size	90 μm (45-165 μm)
Dynamic binding capacity	≥20 mg of 6xHis-tagged protein (27 kDa) /ml settled resin
Storage solution	1X PBS containing 20% ethanol

Reagents Compatible with High Affinity Ni-Charged Resin

Denaturants	Detergents	Reducing agents	Salts	Others
6 M Gu·HCl	2% Triton X-100	20 mM β-ME	4 M MgCl₂	50% glycerol
8 M Urea	2% Tween 20	1 mM DTT	5 mM CaCl₂	20% ethanol
——	1% CHAPS	——	2 M NaCl	1 mM EDTA

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Properties

Storage & Stability

2-8 °C; DO NOT FREEZE

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Applications

Applications:

1. Purification of polyhistidine-tagged proteins under native conditions.
2. Purification of polyhistidine-tagged proteins from E. coli under denaturing conditions.

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Citations

1. Li Liu, et al. A Specific Sinorhizobium Flagellin Suppresses Legume Nodulation Through Immune Activation. Plant Biotechnology Journal. (2026-02)
2. Binita Shah; Moritz Hunkeler; Ariana Bratt; Hong Yue; Isabella Jaen Maisonet; Eric S. Fischer; Sara J. Buhrlage, et al. Structural basis of VCP-VCPIP1-p47 ternary complex in Golgi maintenance. Nature Communications. (2021-02)
3. Binita Shah; Moritz Hunkeler; Ariana Bratt; Hong Yue; Isabella Jaen Maisonet; Eric S. Fischer; Sara J. Buhrlage, et al. Structural basis of VCP-VCPIP1-p47 ternary complex in Golgi maintenance. Nature Communications. (2021-02)
4. Binita Shah; Moritz Hunkeler; Ariana Bratt; Hong Yue; Isabella Jaen Maisonet; Eric S. Fischer; Sara J. Buhrlage, et al. Structural basis of VCP-VCPIP1-p47 ternary complex in Golgi maintenance. Nature Communications. (2021-02)
5. Binita Shah; Moritz Hunkeler; Ariana Bratt; Hong Yue; Isabella Jaen Maisonet; Eric S. Fischer; Sara J. Buhrlage, et al. Structural basis of VCP-VCPIP1-p47 ternary complex in Golgi maintenance. Nature Communications. (2021-02)
6. HsuKuan-Wei, et al. The synergy between RSC， Nap1 and adjacent nucleosome in nucleosome remodeling. Biochim Biophys Acta Gene Regul Mech. (2019)
7. View Runting Li, et al. Characterization of Isoforms of the Ovine Granulocyte Colony Stimulating Factor. biorxiv. (2019)
8. Xu X, et al. Obtaining a mutant of Bacillus amyloliquefaciens xylanase A with improved catalytic activity by directed evolution. Enzyme Microb Technol. (2016-05)